LOCUS       DAXWLB010000001        60953 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXWLB010000001 DAXWLB010000000
VERSION     DAXWLB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.001"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            6..734
                     /gene="purC"
                     /locus_tag="ACGE4H_000001"
     CDS             6..734
                     /gene="purC"
                     /locus_tag="ACGE4H_000001"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFF8363455.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            734..988
                     /gene="purS"
                     /locus_tag="ACGE4H_000002"
     CDS             734..988
                     /gene="purS"
                     /locus_tag="ACGE4H_000002"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFF8363456.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            990..1661
                     /gene="purQ"
                     /locus_tag="ACGE4H_000003"
     CDS             990..1661
                     /gene="purQ"
                     /locus_tag="ACGE4H_000003"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFF8363457.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1662..3884
                     /gene="purL"
                     /locus_tag="ACGE4H_000004"
     CDS             1662..3884
                     /gene="purL"
                     /locus_tag="ACGE4H_000004"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFF8363458.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3869..5308
                     /gene="purF"
                     /locus_tag="ACGE4H_000005"
     CDS             3869..5308
                     /gene="purF"
                     /locus_tag="ACGE4H_000005"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFF8363459.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5331..6383
                     /gene="purM"
                     /locus_tag="ACGE4H_000006"
     CDS             5331..6383
                     /gene="purM"
                     /locus_tag="ACGE4H_000006"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFF8363460.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6380..6958
                     /gene="purN"
                     /locus_tag="ACGE4H_000007"
     CDS             6380..6958
                     /gene="purN"
                     /locus_tag="ACGE4H_000007"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFF8363461.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6955..8490
                     /gene="purH"
                     /locus_tag="ACGE4H_000008"
     CDS             6955..8490
                     /gene="purH"
                     /locus_tag="ACGE4H_000008"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFF8363462.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8506..9780
                     /gene="purD"
                     /locus_tag="ACGE4H_000009"
     CDS             8506..9780
                     /gene="purD"
                     /locus_tag="ACGE4H_000009"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFF8363463.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9902..10816
                     /locus_tag="ACGE4H_000010"
     CDS             9902..10816
                     /locus_tag="ACGE4H_000010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFF8363464.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10949..12514)
                     /locus_tag="ACGE4H_000011"
     CDS             complement(10949..12514)
                     /locus_tag="ACGE4H_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8363465.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12507..13958)
                     /locus_tag="ACGE4H_000012"
     CDS             complement(12507..13958)
                     /locus_tag="ACGE4H_000012"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8363466.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13972..14988)
                     /locus_tag="ACGE4H_000013"
     CDS             complement(13972..14988)
                     /locus_tag="ACGE4H_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8363467.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15208..16296
                     /gene="chvE"
                     /locus_tag="ACGE4H_000014"
     CDS             15208..16296
                     /gene="chvE"
                     /locus_tag="ACGE4H_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8363468.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16350..17876
                     /gene="mmsA"
                     /locus_tag="ACGE4H_000015"
     CDS             16350..17876
                     /gene="mmsA"
                     /locus_tag="ACGE4H_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8363469.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17878..19083
                     /gene="mmsB"
                     /locus_tag="ACGE4H_000016"
     CDS             17878..19083
                     /gene="mmsB"
                     /locus_tag="ACGE4H_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFF8363470.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAVRLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19340..20317
                     /gene="guaC"
                     /locus_tag="ACGE4H_000017"
     CDS             19340..20317
                     /gene="guaC"
                     /locus_tag="ACGE4H_000017"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFF8363471.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20581..22005
                     /locus_tag="ACGE4H_000018"
     CDS             20581..22005
                     /locus_tag="ACGE4H_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8363472.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22047..22304)
                     /locus_tag="ACGE4H_000019"
     CDS             complement(22047..22304)
                     /locus_tag="ACGE4H_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363473.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            22466..23575
                     /gene="pdhA"
                     /locus_tag="ACGE4H_000020"
     CDS             22466..23575
                     /gene="pdhA"
                     /locus_tag="ACGE4H_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFF8363474.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23578..24555
                     /locus_tag="ACGE4H_000021"
     CDS             23578..24555
                     /locus_tag="ACGE4H_000021"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFF8363475.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24580..26223
                     /locus_tag="ACGE4H_000022"
     CDS             24580..26223
                     /locus_tag="ACGE4H_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFF8363476.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEIQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26231..27637
                     /gene="lpdA"
                     /locus_tag="ACGE4H_000023"
     CDS             26231..27637
                     /gene="lpdA"
                     /locus_tag="ACGE4H_000023"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFF8363477.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27799..28074
                     /locus_tag="ACGE4H_000024"
     CDS             27799..28074
                     /locus_tag="ACGE4H_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFF8363478.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28080..28847
                     /locus_tag="ACGE4H_000025"
     CDS             28080..28847
                     /locus_tag="ACGE4H_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFF8363479.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29015..30844
                     /gene="typA"
                     /locus_tag="ACGE4H_000026"
     CDS             29015..30844
                     /gene="typA"
                     /locus_tag="ACGE4H_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFF8363480.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31105..32877
                     /gene="recQ"
                     /locus_tag="ACGE4H_000027"
     CDS             31105..32877
                     /gene="recQ"
                     /locus_tag="ACGE4H_000027"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFF8363481.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            32991..33293
                     /locus_tag="ACGE4H_000028"
     CDS             32991..33293
                     /locus_tag="ACGE4H_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFF8363482.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33296..34459
                     /locus_tag="ACGE4H_000029"
     CDS             33296..34459
                     /locus_tag="ACGE4H_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8363483.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34485..37913
                     /locus_tag="ACGE4H_000030"
     CDS             34485..37913
                     /locus_tag="ACGE4H_000030"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFF8363484.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            37934..39058
                     /locus_tag="ACGE4H_000031"
     CDS             37934..39058
                     /locus_tag="ACGE4H_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFF8363485.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39039..39485
                     /locus_tag="ACGE4H_000032"
     CDS             39039..39485
                     /locus_tag="ACGE4H_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8363486.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39469..39807
                     /locus_tag="ACGE4H_000033"
     CDS             39469..39807
                     /locus_tag="ACGE4H_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFF8363487.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            39926..40486
                     /gene="rsmD"
                     /locus_tag="ACGE4H_000034"
     CDS             39926..40486
                     /gene="rsmD"
                     /locus_tag="ACGE4H_000034"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFF8363488.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTIDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40483..40974
                     /gene="coaD"
                     /locus_tag="ACGE4H_000035"
     CDS             40483..40974
                     /gene="coaD"
                     /locus_tag="ACGE4H_000035"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFF8363489.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            40964..42007
                     /locus_tag="ACGE4H_000036"
     CDS             40964..42007
                     /locus_tag="ACGE4H_000036"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFF8363490.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42166..42894
                     /locus_tag="ACGE4H_000037"
     CDS             42166..42894
                     /locus_tag="ACGE4H_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFF8363491.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43010..43255
                     /locus_tag="ACGE4H_000038"
     CDS             43010..43255
                     /locus_tag="ACGE4H_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8363492.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43252..43470
                     /locus_tag="ACGE4H_000039"
     CDS             43252..43470
                     /locus_tag="ACGE4H_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFF8363493.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43606..44145
                     /locus_tag="ACGE4H_000040"
     CDS             43606..44145
                     /locus_tag="ACGE4H_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFF8363494.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44138..46411
                     /locus_tag="ACGE4H_000041"
     CDS             44138..46411
                     /locus_tag="ACGE4H_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFF8363495.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46449..47495
                     /gene="holA"
                     /locus_tag="ACGE4H_000042"
     CDS             46449..47495
                     /gene="holA"
                     /locus_tag="ACGE4H_000042"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFF8363496.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47584..47838)
                     /gene="rpsT"
                     /locus_tag="ACGE4H_000043"
     CDS             complement(47584..47838)
                     /gene="rpsT"
                     /locus_tag="ACGE4H_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFF8363497.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48238..48507)
                     /locus_tag="ACGE4H_000044"
     CDS             complement(48238..48507)
                     /locus_tag="ACGE4H_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363498.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(48769..48897)
                     /locus_tag="ACGE4H_000045"
     CDS             complement(48769..48897)
                     /locus_tag="ACGE4H_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363499.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(48977..49432)
                     /locus_tag="ACGE4H_000046"
     CDS             complement(48977..49432)
                     /locus_tag="ACGE4H_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFF8363500.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49570..51498)
                     /locus_tag="ACGE4H_000047"
     CDS             complement(49570..51498)
                     /locus_tag="ACGE4H_000047"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8363501.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51695..52528)
                     /locus_tag="ACGE4H_000048"
     CDS             complement(51695..52528)
                     /locus_tag="ACGE4H_000048"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFF8363502.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52720..53424)
                     /locus_tag="ACGE4H_000049"
     CDS             complement(52720..53424)
                     /locus_tag="ACGE4H_000049"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8363503.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53447..54073)
                     /locus_tag="ACGE4H_000050"
     CDS             complement(53447..54073)
                     /locus_tag="ACGE4H_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8363504.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54399..56774
                     /locus_tag="ACGE4H_000051"
     CDS             54399..56774
                     /locus_tag="ACGE4H_000051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFF8363505.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56798..57289)
                     /locus_tag="ACGE4H_000052"
     CDS             complement(56798..57289)
                     /locus_tag="ACGE4H_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFF8363506.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57476..58396
                     /locus_tag="ACGE4H_000053"
     CDS             57476..58396
                     /locus_tag="ACGE4H_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8363507.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58550..58894
                     /locus_tag="ACGE4H_000054"
     CDS             58550..58894
                     /locus_tag="ACGE4H_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFF8363508.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59084..59584)
                     /locus_tag="ACGE4H_000055"
     CDS             complement(59084..59584)
                     /locus_tag="ACGE4H_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8363509.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60112..60195
                     /locus_tag="ACGE4H_000056"
     tRNA            60112..60195
                     /locus_tag="ACGE4H_000056"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60146..60148,aa:Leu,seq:cag)
     gene            complement(60519..>60953)
                     /locus_tag="ACGE4H_000057"
     CDS             complement(60519..>60953)
                     /locus_tag="ACGE4H_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8363510.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        19991 a        10541 c        13085 g        17336 t
ORIGIN      
        1 cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata tatcttactt
       61 ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg aatggcgcga
      121 ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt gcaattttcc
      181 gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct aaaaccgaac
      241 aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat attgcagcag
      301 gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg
      361 aattctacta taaggatgac cagttggatg atccatttat caatgaagag catatctatt
      421 tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg ttggctatta
      481 atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat ttcaagctag
      541 aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg gatacttgcc
      601 gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc agagatctag
      661 gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta actgaagaaa
      721 aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt cagtgttaga
      781 tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga
      841 tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac caatcgatac
      901 agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa cataccgata
      961 tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt
     1021 caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct gatgccgaat
     1081 ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg ccgggaggat
     1141 tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca atcatggaag
     1201 aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat ggattccaga
     1261 tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct ttgcatttta
     1321 tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca gagtatgaag
     1381 ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc tgtgacgaag
     1441 aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat gacaacatca
     1501 acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg ttaggaatga
     1561 tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg aaacggttct
     1621 ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac
     1681 cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg actgatgaag
     1741 aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg gaaactggac
     1801 tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg gttctacgca
     1861 aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg
     1921 acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat ccttcagccg
     1981 tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat attttcagca
     2041 tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta gataatccaa
     2101 gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc aattgtatcg
     2161 gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga aacccattag
     2221 tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg caagccgctg
     2281 gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt atccacggag
     2341 caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct gctgtacaag
     2401 taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta atcttgcatc
     2461 attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt tcatctagtg
     2521 cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa gtcccacaaa
     2581 gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa cggatgttga
     2641 tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag tatgatctag
     2701 atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat catcaaggag
     2761 aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt tatcataaag
     2821 aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat cagccagtta
     2881 tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt gcttcaaaac
     2941 gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc cgtccaggaa
     3001 gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg accacagact
     3061 gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca gtggcagaag
     3121 ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac tgcctgaact
     3181 atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct gacggaattg
     3241 ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct ttatataatg
     3301 aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga ttgattgagg
     3361 atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc tatatattag
     3421 gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt ggaagaatcg
     3481 aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag ttagtattga
     3541 cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa
     3601 ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag tttgaattgt
     3661 cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc gcaccggaaa
     3721 ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt aaggtgacgg
     3781 atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa actgcagtag
     3841 caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga
     3901 atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat acttcgggct
     3961 ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa
     4021 gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc aaagaaaatt
     4081 gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg
     4141 aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga tcggcttagc
     4201 gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg aaaatggggc
     4261 gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac gaagtgaaga
     4321 aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta
     4381 tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt ttcgtccatt
     4441 agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt gtgcattaga
     4501 agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga tcatcaatga
     4561 cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt gttcgatgga
     4621 atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc atactgcacg
     4681 gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata tggtcgttgg
     4741 cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg gaatccctta
     4801 tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc caacgcctga
     4861 gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag tggaaggtaa
     4921 aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac ggatcgtcca
     4981 tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc ctcctttgaa
     5041 atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg cggcaaatca
     5101 cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt taagtgaaga
     5161 gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat tgtgtatggc
     5221 ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat atcaggaatc
     5281 actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat atgaattcaa
     5341 tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa gtagtagaac
     5401 ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct ataggaggat
     5461 tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt gtctcgggga
     5521 ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac gatacaatcg
     5581 ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca gagccgttat
     5641 atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa aatgtagtaa
     5701 aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga gaaacagctg
     5761 aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct gtagggatag
     5821 ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa
     5881 tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc
     5941 agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat
     6001 tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa
     6061 taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg cgaatgttac
     6121 cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca atattcggct
     6181 gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc aatatgggca
     6241 tcggtatggt cctggcagtc caagaatcga aagtaaaaga aatagtacac gaaattgagc
     6301 aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg
     6361 tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg gaagcaactt
     6421 tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg actggttgtt
     6481 ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg taccggcaga
     6541 ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa
     6601 attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa tcattggacc
     6661 ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc ttttacctgc
     6721 cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt
     6781 cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc aggaaaaagt
     6841 gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata gagtagagca
     6901 tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca
     6961 agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca aggtttgatt
     7021 gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga taaagctggg
     7081 atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga tggccgtgtc
     7141 aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct ccctagccat
     7201 atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt caatttatat
     7261 cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat tgaaaacatt
     7321 gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt tgtcacctct
     7381 gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta tgggcatact
     7441 tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc ggcatatgat
     7501 gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga aaaattgaca
     7561 cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca agccgctgcc
     7621 ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca actccatggg
     7681 aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat catgaaggaa
     7741 tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat tgggacagca
     7801 tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc aatctttggt
     7861 gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat gcataagctg
     7921 tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt actgcaaaaa
     7981 aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca aaatcgagaa
     8041 gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc aatagaaaat
     8101 gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc aaaagcatta
     8161 gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa
     8221 cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt aaaaattgcg
     8281 attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc ttattttcca
     8341 atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat tcagccagga
     8401 ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct agcaatggtc
     8461 tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa cattttggtg
     8521 atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag tccgaaggtg
     8581 gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat caattgtgtc
     8641 gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga ggctattgat
     8701 tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga ttttatggcg
     8761 gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt
     8821 tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag aagttttgaa
     8881 tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg
     8941 gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga agcgctggat
     9001 gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa aatcgtcgta
     9061 gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa taccaaggtg
     9121 tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa agggccaaat
     9181 acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa
     9241 gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg acgctctttc
     9301 acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt gatcgaattc
     9361 aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt
     9421 gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg gcagcaagaa
     9481 ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata tgagacagga
     9541 atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg agtttcctca
     9601 aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc atacggtgag
     9661 acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac gacagatgga
     9721 acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa
     9781 atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc gattttagtg
     9841 aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag gaggagttca
     9901 gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga tcttaggctg
     9961 tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt atgaaaacgg
    10021 cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag aaatctttgc
    10081 agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa caaaatgtgg
    10141 aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat ctgtagaaaa
    10201 aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc gtccagatac
    10261 attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt caggaaaagt
    10321 tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt
    10381 tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg gaatgatcga
    10441 tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg gtagtgtact
    10501 tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc agtatggatt
    10561 ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga
    10621 actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga ttttacgcca
    10681 tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca aggaaatcgc
    10741 tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc gtgctgctgg
    10801 aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat taaaaagaca
    10861 gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat tgcaaatgga
    10921 aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg aaaagctgtg
    10981 tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg aaatagttag
    11041 ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat tgctgcaata
    11101 atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt tggttaaacg
    11161 attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga gcgacccaag
    11221 ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca tggttcaaaa
    11281 ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa gaaatgaaca
    11341 tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg
    11401 atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca tgctggagta
    11461 gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg
    11521 tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga
    11581 ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc
    11641 tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg atctcaaaag
    11701 atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa aaattatttt
    11761 tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg gtctcgatca
    11821 cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa acagcagaga
    11881 agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga tgagcgtaat
    11941 cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt ttccctgcta
    12001 aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt gagcctttga
    12061 acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa gaagtgtgcg
    12121 tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt ttcaattcgc
    12181 tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct cctttcatat
    12241 aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt agtttccttg
    12301 cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc agaatgatgt
    12361 cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct tcgccgacat
    12421 aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga
    12481 ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat tgttttggga
    12541 gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc aatcgtgata
    12601 ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta aagtccgtag
    12661 ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc tgccagtttt
    12721 tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc atcaattttg
    12781 catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg gtatacagca
    12841 ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag tgtttctggt
    12901 atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac tagataatca
    12961 ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct taaagacgta
    13021 cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca gtcttgttcg
    13081 atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat ctgagcttgc
    13141 aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc ccgtaaagac
    13201 tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt
    13261 tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga catctcgatc
    13321 agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt tttcgcatag
    13381 aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag cgagcggaca
    13441 agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc ttgtaagata
    13501 tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt ggtatccata
    13561 aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag tgtatcgatc
    13621 gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt atttttgatc
    13681 tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc atctacggtt
    13741 acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac cttcaaagca
    13801 attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa
    13861 tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc tttgtttgct
    13921 tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca gtcaataatt
    13981 tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa taaaaatatc
    14041 tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg tattggctac
    14101 ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg agcgtagctt
    14161 ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc caggtttgat
    14221 tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat ggctgtataa
    14281 tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt cttttccttt
    14341 catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt tatggtcgat
    14401 ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc cttcggagcg
    14461 tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga tttcctgttt
    14521 ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa
    14581 actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat gtctgtcgac
    14641 aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc acccggattc
    14701 ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc gtatatcttg
    14761 gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat cttctttttc
    14821 aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg ttcctgattg
    14881 cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac accccatcat
    14941 ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt taaatcactc
    15001 catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt caaaaggaat
    15061 caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa
    15121 atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag ctatactcgg
    15181 tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat tgttgggact
    15241 atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg atctgcagat
    15301 gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag atggatcgct
    15361 gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga tcttcaatat
    15421 ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac aaaaggggta
    15481 gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt agctaaagca
    15541 aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc agaaaatgtc
    15601 gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc ttatattgaa
    15661 gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt tggtggctcg
    15721 cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt acaaccatat
    15781 atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca aatcgctacg
    15841 cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag cgcaaattat
    15901 actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt aggaatcatt
    15961 tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt gattactgga
    16021 caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac acaaacgatc
    16081 tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat
    16141 ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa aactgtccct
    16201 acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga gctgatcgac
    16261 accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa ggaactaaag
    16321 agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa atggcagata
    16381 tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg attgaacgtg
    16441 gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg atgaatgtct
    16501 tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat ggaaagctgt
    16561 gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt catcaagaat
    16621 tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat gaacaagcag
    16681 aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg ctgaataaag
    16741 tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg caacagcaac
    16801 tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg gatgagccta
    16861 ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga gagttgaaag
    16921 agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg gatatcgcag
    16981 atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat gaaatgatca
    17041 gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt tatcctgaaa
    17101 gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc tatcaccctc
    17161 ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa ggagagattg
    17221 taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt ctatttggac
    17281 attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag atatcagtta
    17341 agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt
    17401 taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt ttggataaaa
    17461 taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa aatttccgga
    17521 aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt agtggtggga
    17581 atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt ctttttttgg
    17641 atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg attatcgaag
    17701 aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct gaaatattag
    17761 ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa gtctcgcgag
    17821 aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg ggttaagttg
    17881 gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa aagtagagca
    17941 gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact tattgcgttt
    18001 caattgatga cggatggtat tttctggcgt ccattgaata taactaacct tgttctgcaa
    18061 aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt aggtcatgtg
    18121 gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc
    18181 aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc
    18241 ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat cgtgacttta
    18301 gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca atctctagca
    18361 ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt aagcgaagga
    18421 agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt gttagttgtt
    18481 ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga atctccaaca
    18541 gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac ttatatcttt
    18601 gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt tgcagcatat
    18661 gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg cggaaatcga
    18721 aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt attcgtcaac
    18781 atgggagtca tggcagcttt agctggatta gttttagctg ttcgattgaa tgcggcaaca
    18841 cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt tggtggtgct
    18901 tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt
    18961 gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca ggcaatcaaa
    19021 ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa acgaacaacg
    19081 taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca cagtcttttt
    19141 ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat atatgttttt
    19201 ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg agttaaatta
    19261 catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg ctggatgtga
    19321 acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat taattcctaa
    19381 taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag gtaaacatac
    19441 attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa caatcgcaga
    19501 attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag cagctcgaat
    19561 tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa
    19621 gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc ctgattatat
    19681 cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc agcatattaa
    19741 aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg
    19801 tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag gaaaagtatg
    19861 tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag ctcttagatg
    19921 gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa cacatggcga
    19981 tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat tgtttgccgg
    20041 tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata aagaatactt
    20101 tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg
    20161 gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag accttcaatc
    20221 atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg actatgtcat
    20281 cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa tattaggatg
    20341 ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat agttctttat
    20401 atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc attattcaga
    20461 ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc
    20521 ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt
    20581 atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat ggaacagatt
    20641 atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc agaacgaaaa
    20701 ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga agagcttagg
    20761 gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca gactgaatgg
    20821 gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg ttatgagcag
    20881 acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac atcgtttctt
    20941 gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt tccttcttat
    21001 tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta tactccgtta
    21061 cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc ccccaaccag
    21121 cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac gattctttca
    21181 gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc tcttttcaaa
    21241 gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga cttagaaaaa
    21301 ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc gaattttcaa
    21361 aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga
    21421 cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt tctaaaccaa
    21481 gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt cggttcgtta
    21541 tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga gacttttatc
    21601 tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct tacacaaatc
    21661 acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag
    21721 ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt tttcaagctt
    21781 cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa actagacccc
    21841 gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct actttttggg
    21901 caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca aataccgttg
    21961 ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca caaagtaaat
    22021 agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt tttcggaaaa
    22081 agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt
    22141 aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa tagaaacaga
    22201 tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaatataaaa cgctttcctt
    22261 attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc ctttcatcgt
    22321 gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat taatgaagac
    22381 ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga gattttctta
    22441 tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc
    22501 actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc aagatggaaa
    22561 cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg aattaatgac
    22621 tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc gccaaggacg
    22681 cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta gtgcttacgc
    22741 atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac tagtaaaaca
    22801 tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag gtaattctta
    22861 tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat atgtacaagc
    22921 agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac
    22981 tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg ctggcgctta
    23041 ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta cacctcgtga
    23101 aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga ttcctggtat
    23161 tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg cacgggaatg
    23221 ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt atggaccaca
    23281 tacactatct ggtgacgatc caacccgcta ccgtacaaaa gacacagatg acgattggca
    23341 gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac tttggtctga
    23401 agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag ctattgctga
    23461 agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc
    23521 tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga aataatcatg
    23581 gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact agaaaatgac
    23641 gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact
    23701 gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt agctgaatct
    23761 ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc
    23821 caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca
    23881 agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc atttggtggc
    23941 ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc tcagtctcct
    24001 ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt gatttcttct
    24061 attagaagta acgatccggt tgttttccta gaacacatga aactatatcg ctcattccga
    24121 gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt tactcgtgag
    24181 ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat caaagcagca
    24241 gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac agttgcgcca
    24301 ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa
    24361 gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc agaacgcgct
    24421 atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt cttcccattt
    24481 ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa
    24541 atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca atttaaattg
    24601 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    24661 gatacgatca atgaagatga tacattacta gaaattcaaa atgacaaatc agtagaagaa
    24721 atcccgtcac ctgttacagg aacggttaaa aatgtgattg ttccagaagg taccgtagca
    24781 aatgttgggg atgtattagt agaaatcgat gcaccaggac acgaagacaa tgaaggggac
    24841 agtggtgtag ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc cactgttgat
    24901 acagaatcag ctggatcgtc aagcgaagga gtcttccaat ttaagttacc ggatatcggt
    24961 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
    25021 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat tccgtcacct
    25081 gtcacaggga cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat
    25141 gtattagtag aaatcgatgc tcctggacac aatagtgcac cgtcaactag tgcgccatca
    25201 gctgaagcac caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc
    25261 gatccaaata aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat
    25321 gtagatatct cacaagtaac tgcaactgga aaaggtggca gagtaacaaa agaggacatc
    25381 gaaaacttct tggcaggtgg tccaagcagt gcaccagcga aatcagaagc gccagaagcg
    25441 gcagcaccga aagaagcagc tcctgcagca gaaagcaaac cagctgcacc agcaaaacca
    25501 ttcaagtcca acttaggaga cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct
    25561 atcgctaaag caatggtcaa cagtaagcat acagctccac acgtgacatt gcatgatgaa
    25621 gtagaagtgt ctaaactatg ggataatcgg aaacgattta aagaagttgc tgctgcaaat
    25681 ggtacgaaac ttactttctt gccatatgtc gtgaaagctt taacagcgac tgttaagaaa
    25741 tacccagtat tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta taaacattac
    25801 tacaatatcg gtattgcaac tgatacggat cacggactat atgtaccaaa tgtgaaagat
    25861 gcagaccgca aaggaatgtt tgctattgca gacgaaatca atgagaaagc aaaattagct
    25921 catgacggca aactatctgc agaagatatg cgcaatggaa caattacgat cagcaatatc
    25981 ggatctgtag gtggcggttg gtttacacca gtcatcaact atccagaagt agctatttta
    26041 ggtgtaggaa cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt
    26101 cgtgtgatga aactgtcatt aagtttcgat caccgtatcg tagatggagc aactgcgcaa
    26161 caagcaatga ataatatcaa acgtctttta gctgatccag agctattaat gatggaagga
    26221 tgataaacaa atggtagttg gagattttgc tgtagaatta gatacagttg taattggtgc
    26281 gggacctggt ggctatgtag ctgccatccg cgctgctgaa atgggacaaa aagtagcaat
    26341 cattgaaaga gagtatattg gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc
    26401 actgatagct gcaggacacc attatcaaga gtcactagat tctgaaatgt ttggagtaac
    26461 tagtgaaaac gtcaaactag attttgcaaa aacacaagaa tggaaagaaa acaaagttgt
    26521 ccatacgttg acttctggtg ttggtttcct attaaaaaaa cataaggttg aaacaatcga
    26581 aggagaagcg ttcttcgtag atgatcacac actacgcgtc attcatccag attcagctca
    26641 aacttattcg ttcaataacg caatcatcgc aacaggttcc cgtccaatcg agattccagg
    26701 atttaaattc ggaggacgtg tgttagattc tactggcgga ttagcattga aagaagtacc
    26761 taagaaattt gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc
    26821 aaacttagga gcagaagtaa cgattcttga aggctcacca caaattttac caacttacga
    26881 aaaagatcta gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac
    26941 gaatgcaatg gcgaaagaag cagtcgataa cggtgacagc gtgacagtga aatatgcagt
    27001 ggacgggaaa gaagaatccg ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc
    27061 gaatacagat gatatgggct tagaacaagc gggtgtcgaa gtaggagaac gaggattgat
    27121 cacggtggat aaacaaggac gtacgaacgt acctaacatc tatgccatcg gtgatatcgt
    27181 tccaggggcg gcattagcac acaaagccag ttatgaagca aaaatcgctg ctgaagcaat
    27241 ctctggcaaa aaagtagctg tagattataa agcaatgccg gcagtagcct tcactgatcc
    27301 tgaattggct tctgtaggta tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa
    27361 agcatataaa ttcccatttt cagggaacgg tcgggcatta tcgcttggta aaacagaagg
    27421 attcatccgt ctagttacga cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg
    27481 agtcggcgca agtgatatgg tctctgaatt agctttagca atcgaatcag gaatgaatgc
    27541 tgaagatatc gccttgacga ttcatcctca tccatctctt ggcgaaatcg tcatggatgc
    27601 ttcggaatta gcattaggtt taccaatcca tatttgattt gaataataca ggcgaaacgc
    27661 aactgaaaaa tcagttgcgt tttgtttttt agaattatca ataatattat tatgtaaact
    27721 taaaaatatg acaacttctt ggtttattac cacttcctat gctacactga agaggaatat
    27781 gaaataggag agttgaagat gcaagaatat cagtatccac ttgatttaga ttggacaaca
    27841 gacgaaatgg tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg
    27901 ataaacaatg aagctttttt gaagacttat cagcagttta aaactgttgt caaatccatt
    27961 ggtgaggaaa aacgattggg acgggaattt gaaaaagctt ccggttattc tttatatcgt
    28021 acagtgcagc aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg gtgagataaa
    28081 tggaacagat gattgatcat atcaagtcct ggcttgaaga agctggtgag aaaatcaaat
    28141 cttctcaaca aacattaaca atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg
    28201 tcgatcgaaa catccagaat tttctaatcg aaaaaatcca ttcctttgat ccagaggcga
    28261 aaatattggc agaagaagaa gggtgtaatc aattatcgga tctcgatgga agagtcttta
    28321 tcatcgatcc tattgatggc accttgaatt ttgtgattga aggggaaaat ttttgtatta
    28381 tgctcgctgt ttatgaatcc ggcgttggaa agcttggttt catttatgac gtgatgagaa
    28441 atgaactttt ttggggtgga aaaggaatag gcgtgtataa aaatgatgag ccattgccta
    28501 gaccagcaaa taaatcctta tcggaaggct tacttggtgt gaatagttat cttttcggtc
    28561 ataataaata tggaatccga gaaattgcgg aaagcagcat ggggacacgt atgtacggat
    28621 gcgctggtct tgaactagtg gcaatcttga aaggaaacca cattgcttat atttcaaacc
    28681 ttagcccttg ggattatgca gcgggaaatg tcttattgga agagtttggt atggtaacaa
    28741 gcggtctttt tggtactcct ttgacatttg aaaaaaggga atactattta gcagggactt
    28801 cttgtgcata tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa
    28861 atcattcaca aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca
    28921 ttttactata tttcatgaaa aaagtctgct ataattagca gtagagtaaa atttgccctg
    28981 tcataaagaa ccaataaaaa aggagttagt tacattgaat tatagaaacg atatccgtaa
    29041 cgtcgcaatt attgcccacg ttgaccacgg aaaaacaaca ttagttgatg aactattaaa
    29101 acaatcagat acattagacg cacatacgca attgcaagaa cgtgcgatgg actcaaatgc
    29161 gctagaaaaa gaacgtggta tcacgatttt agccaaaaac acagctgtag actataaagg
    29221 tattcgcgta aacatcatgg atactccagg acacgcggac ttcggtggcg aagtagaacg
    29281 gatcatgaaa atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc
    29341 tcaaacacgt tttgtattga aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt
    29401 aaacaagatt gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact
    29461 attcatcgaa ttaggagcag atgatgatca gttagacttt cctgtgattt atgcatctgc
    29521 actaaatggt acgtcaagct tgtcagatga tccagcagat caagaaccaa ccatggcacc
    29581 tatttttgat acaattattg aaaaaatccc tgctcctgtc gataacagcg acgaaccatt
    29641 acaattccaa gtttcattac ttgactacaa cgattatgta ggtcgtatcg gaatcggacg
    29701 tgtatttaga ggaacaatca aagtaggaga ccaagtagca ttaattaaat tagacggaac
    29761 agttaagaaa ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac gtctagagat
    29821 tcaagaagca aaagctggag acttgattgc tgtctcaggt atggaagata tctttgtcgg
    29881 tgaaacagtt actcctgtgg atcatcaaga tgcgttacct atccttcaca ttgacgaacc
    29941 aacattgcaa atgactttct tagttaataa ttcaccattt gccggtcgag aaggtaaatt
    30001 cgtaaccgct cgtaagattg aagaacgttt gatggcagaa cttcaaacag atgtatcctt
    30061 acgagttgaa ccaacaaact ctccagatgc atggacagtt tctggtcgtg gagaattgca
    30121 tttgtctatc ttgattgaaa acatgcgccg tgagggatac gaattgcaag tatctcgtcc
    30181 agaagttatt gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat
    30241 cgatacacca gaagaatata tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga
    30301 aatgcaagat atggtccata caggaaacgg acaaatccgt ttgacattct taacacctgc
    30361 ccgcggtttg attggttatt caactgaatt cctttcaatg acacgtggtt acggaatcat
    30421 gaaccatact tttgaccaat atctgccaat gcttcctggt caaattggcg gacgtcacca
    30481 aggggcgctt gtttcgatcg atacaggaaa agcaacgact tattctatca tgagtatcga
    30541 agaacgcggt acagtcttcg tagaaccagg tacagaagta tatgaaggaa tgattatcgg
    30601 ggaaaacagc cgtgataatg atttgactgt caacatcacg aaagcaaaac aaatgacaaa
    30661 cgtacggtct gctactaaag accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt
    30721 ggaagaatct ttggaattct tgaatgatga tgaatattgc gaagtaacac cagaaagcat
    30781 tcgcttgaga aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa
    30841 ataatctgta aaaggatgaa gagagaaacc agcttgttat aaaaaatagc tggtttcttt
    30901 ttttgtccaa ctcttttctg tggagaagtc atctttgtca atggacataa attcagaaaa
    30961 tacgccgaaa gacgtttaaa agacttttaa aaaacttatt tctagagagt ttttgtttca
    31021 taataagata atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa
    31081 atggcaagag aaaggagcgg gaagatgaac gacttattaa aacaatactt tggttatgat
    31141 gagtttagac ctggtcaaaa agaaattatc cagaaagtca ttgatcagga aaatgtatta
    31201 ggaatcatgc ctaccggaag tgggaaatca atctgttacc agcttccagc tttactatta
    31261 gatgggctta ctgtggttgt gtccccttta atctctttga tgaaggatca ggtggacgcg
    31321 gcgaatcaat taggtatacc cgccactttt atcaatagct cgttagatgg ttacgaaaca
    31381 gctagacgct tccaagaaat cgatagacag cagtaccgtt tactttatat cgctcctgaa
    31441 cgttttatca tgccggattt tatccaagca atgaagcgat ggaacgtccg tatgatcgct
    31501 attgacgagg cacattgtat ctctcagtgg gggcatgatt tccgcccaag ttatttacag
    31561 atggcgaacc agctagatca actgccaaat cgtcctgtaa ttgttgcttt gacagctaca
    31621 gcgactgtgc aagtagctgc tgacataaaa agacttttaa aaattccaga aaacaaccat
    31681 attcagactg gttttgaacg agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa
    31741 gaacaatatc taatcgaata tttaaagatc aacaaaaatc aatctggtat catttatgca
    31801 gcaacgagaa aagaagtaga ccgtctctat catttgctta aaaaattcga tttttccgtg
    31861 ggtagatatc atggtggtct aaatgagaat gagcgaacag aaatgcaaga agccttttta
    31921 tacgatcgct tgcagctgat tgtagcgaca aatgcatttg gtatgggtat aaataaaagc
    31981 aatgtccgtt ttgttatcca ttatcaaatt cctggctctc tagaagcata ctatcaggaa
    32041 gctggtcggg cgggaagaga tggtctttcc agtgaagcca tcctgctttt ctctcctcaa
    32101 gatatccaag tccaaaagtt ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa
    32161 aaagaatatg aaaaattaaa agcgatgaca gaatacgtgt atattgagtc ctgtcttcag
    32221 caatacatcc taaactattt tggcgagaca agcagtccgt gcaaccgttg tggtaactgt
    32281 ttagatgaca gagaaatagt agaagtaacg acagaagcac agatggttct ttcttgctta
    32341 aaacgtatgg gtgagaacta tggtaaacaa atgctgatga aagtcttagc aggatcgaaa
    32401 gaacaaaagc tgcaagcttt aggttttgga catttatcta cctatggact gatgaaaaac
    32461 caatcgcaaa aagaaacgat gcagttaatt gaatatttga tatcgaatgg ctatcttctg
    32521 accatcaatg gtgagtatcc tgttttgaaa gtaacagaaa gagggataca agtattgaaa
    32581 ggacaagaat ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa
    32641 accgatacac tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc
    32701 gttcccccgt atgtcgtatt ttcagattcc acattgaaag aaatgagccg aatacgtcca
    32761 tcaagcagat tggagatgct tcagataaaa ggagttggcc aaagcaagct tgataaatat
    32821 ggcgaagcct ttttatctcg gataaaaaat gcggactcta acgttctgga taagtagaaa
    32881 gaacgaatac gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt tgctccttat
    32941 gaaaatcaaa tatgttataa ttaaggaatt agaatgggag gtaagcacag atggattctt
    33001 tgtcgaaaaa acttgcgatc caagtattaa atgatgaagc agaacggatc aaacgattaa
    33061 tccgaaatca aaacaacagt ctctgtatct cacaatgcaa agccttcgaa gaagtggtag
    33121 atacacagat gtacggcttt tcccggcaag tcggttttgc tgtccgtata ggagtaatcg
    33181 gaaagaacga gggccagctg atgttaagtg agctagaacg agaattgaat cagttatata
    33241 gtgaggttta ccaagaaatt tatgataaaa aagaagttgg caaggaggaa tagacttgcc
    33301 taagaaagtg aaaaaaaggc atctattaga ctatagcatc ttgattccct atttaatttt
    33361 atgtgtattg ggattgatca tggtatatag ttctacgtcg tatctattat tagaaaacgg
    33421 acaaaatccg tccgcatcag tcattaacca gagtattttt tgggtgctta gcttgattgc
    33481 tatcgcacta ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc
    33541 agccatcgca gttttgacga ttttgctgct gattgttgtg ttttttggta aggagatcaa
    33601 tggggcgaaa ggttggctgc aaattgcagg attctcgatc caacctgccg aatacctaaa
    33661 aatcatttct atctggtacc tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa
    33721 agattttctt gggactgtga aacgtccact tgcaatggtt atcggactta ctgcattagt
    33781 cgctattctt cccgattttg gtaatgcggc agttatcttt ttgattattt tggttctgct
    33841 tttagccagc ggagtcaatt atgtgtatac tttgattgta ggagtaggag gcttttgtct
    33901 aagtactttt acgatctggc tgatcaatat cacaaacgga aagatcttac ccggacgttt
    33961 acagtatatc tataatcgat ttgctattta ccaaaatcct ttctcagacg aattaaataa
    34021 agggcatcaa ctagtcaacg gctactatgc catgttcaac ggaggactat ttggcagggg
    34081 attaggtaac agtatccaga aaaaaggatt tttacaagaa gcacaaaccg attttattta
    34141 tgcaatcgta gtggaagaac tgggagtgat tatggggatt ttgatattag cgttgctatt
    34201 ctttatgatt gtgcgcattg tccttgtagg tatccgttca aaagatccgt tcaattcgct
    34261 tttgtgcatt gggatcggcg caatgttttt gatccaagta tttgtcaatc ttggaggaat
    34321 tactggcgtt attcctttga cagggatcac gttcccattt ttaagtcaag gaggctccag
    34381 tttgctgatg ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg
    34441 caagagctta ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag
    34501 ttgctaacag gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta
    34561 aaactgttgg aatctatgca gaagaagatg aatattccgt acaccgtttc aaagcggatg
    34621 aagcatattt agtaggacaa ggaaaaaaac cgattgatgc gtatctagat attgaaggga
    34681 tcatttctat cgcgaaagaa tgcggagctg acgctattca tccgggttac ggtcttttgt
    34741 cggaaaactt gaattttgcg caacggtgcg aagaagaagg catcattttt gttggaccca
    34801 aactgcatca tttagatatt tttggagata aaatcaaagc aaaagctgca gctatcgaag
    34861 cggggattgc ttcgattcca ggcacagatg gaccaatcgc ttctatagac gatgccttgg
    34921 agtttgcgaa acaatacggt tatccgatta tgatcaaggc agctctaggc ggcggcggac
    34981 gcggcatgcg tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa
    35041 gcgaagcaaa agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc
    35101 ctaagcatat cgaagtacag attctcgggg atacgcatgg aaacgtcatc catctttttg
    35161 aaagagattg ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct
    35221 ctatgaatga acagcaacgt caaaaaatct gccaggcagc tgtccaattg atgaaatatg
    35281 tcgggtatgt caacgcaggt acagtagaat ttcttgttga aggagatgat ttttacttta
    35341 tcgaagtcaa ccctcgcgtc caagtggaac atacgatcac agaaatgatt acagatattg
    35401 atatcgtaac aacgcaactg ttgattgctc aaggtttgga tctgcataaa gaaatcggat
    35461 tgcctcagca agaaggaatt aaattaaatg gttcagccat tcaatgccga atcactacag
    35521 aagacccgtt gaataacttt ttaccagaca cagggaaaat cgatacgtat cgttcacctg
    35581 gtggttttgg cgtacgtctg gatgtcggaa atgcttacgc gggctatgta gtgacaccgt
    35641 attttgattc attgctagta aaagtatgta cgcatggtgc tacctttgaa acagccattc
    35701 agaaaatgga acgttgcttg aaagaattta gaatccgtgg agtgaaaaca aacattccat
    35761 ttatgctcaa tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa acaaccttca
    35821 ttgatagtac agcaacatta tttgaatttc ctcgactacg agaccgcgga aacaaaacga
    35881 tgaaatatat cggtgaaatt actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac
    35941 cattttatga agaaccacgt atgcctaaag atctaatcac aagaactgat tacgtaacgg
    36001 ctaagaatgt attagatgcg aagggtgccg atgctttggt cgaatggatc aaaggacagg
    36061 aaaatttgtt gttgacggat acgacattta gagacgccca tcaaagtctg ctagcaactc
    36121 gtgttcggac aaaggatttc aagcagattg cacgtttgac tggagaaggt cttcctgaac
    36181 tcttttcaag tgagatgtgg gggggagcaa catttgatgt ggcttaccgc tttttgaatg
    36241 aagatccttg gcaacggttg agaaaaattc gttctttaat gcctaatacg ttgctgcaaa
    36301 tgcttttcag aggttcaaat gccgttgggt attctaatta tcctgacaat gtattggtgg
    36361 aatttgtcaa agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga
    36421 actggactcc acaaatggaa aaaagtatcc aagctgtacg ggatactggt aaaatcgcag
    36481 aggcagctat ttgctatact ggcgatatca acgatccaag tcgggcaaaa tacaatgttc
    36541 agtattataa agacatggca aaggaactag aacaactggg tgcgcatatc attgcaatca
    36601 aagatatggc tgggctgctt aaaccgcagg cagcttatcg tttgattagt gaattgaaag
    36661 aaacgacaga tctgccaatc catctgcata ctcatgatac cagcgggaat ggaatcatca
    36721 cttattctgc tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg agtgcaatga
    36781 gtgggaatac tagtcagcct agcatgagca gcttgtatta tgcactggtc aatggtccac
    36841 ggctgccaga aatcacgatt gaaaatgcac agaaattaaa ccattattgg gaagatgtgc
    36901 gcatgtatta caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc
    36961 atgaaatgcc aggaggacag tattcgaatc tacagcaaca ggcaaaagct gtcggattag
    37021 gtcatcgctg ggatgaaatc aaacaaatgt atcatactgt caacttgatg tttggcgata
    37081 tcgttaaagt aactccatca tctaaagtag ttggagatat ggcactattt atggtccaaa
    37141 atgatcttac agaagaagat atttatgaaa aaggcgaaac actaagtttc cctgaatctg
    37201 ttgtgacttt cttccaagga gaactagggc aacccgttgg cggatttcct aaaaaattgc
    37261 aaaagattat tcttaaaggc agacctgcat tgaatgaacg tccaggtctt ttggctgaat
    37321 cagtcgactt caacgaagta aaaaaagaat tggcagaaaa aattggctat gaaccaaaac
    37381 aagacgaagt attaagctat ttgatgtatc cgcaagtctt tttggactat caaaaagcat
    37441 ataatcaatt tggcgatgtt acattactgg acacaccaac atttttccaa ggtatacgtt
    37501 tgggtgaaac aatcaatgta caaatcgaac gcggaaaaac gcttatcatt cgtttagatg
    37561 aaatcggaga acccgatatc gaagggaatc gcgtactgtt cttcaatttg aatggacaaa
    37621 gacgtgaaat cactatcaaa gataattcga tcatcagtac ggtccaaacc cgattgaaag
    37681 ctgaaccgac aaaccgagaa caaattggag caacgatgtc tggttctgtt ttggatgtat
    37741 tagtgaaaaa aggcgataac gttaaaaaag gtgatacgtt gatgattact gaagcgatga
    37801 aaatggaaac ggccatcgaa gcacgttttg acggagaagt cgcacatgta tatgtgtctt
    37861 caggagacac tatttcgtct ggtgaccttt tgattgaagt gactgagaaa taataagcga
    37921 aaggtgggga aacatgaaaa gactgggttt gttttggctg attctgctat tagttattgt
    37981 ggcattctac atggaaccag tcttgtttcc gccaaaactt gaagtgaagg aaaactctga
    38041 tcaacaaata tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag caactggttt
    38101 ttctacttat atcggaaaac cgatcgatca gctggaaaag gattttggcc aagcctatga
    38161 tcgattaact agcggatttg gttttgaaac aagatattat gaaaataaag ctagtcatct
    38221 cacatttgaa gcgaacatcc aagacagcag agtcacaact gttaaagtac tgcaaacagg
    38281 gcctgctgat atcgcgccgt tttatttagg gatgacgatg caagatctta cgaaaatcac
    38341 tactatctat actaatttca cttttgagta ccatgataca gaagttggaa tcgaactaat
    38401 ggaagaggat atgaattatc gtccgctaat tgcttttgat aacgacactt ttgctatttt
    38461 attttttgat caaacaacaa acggcctatt ttcagttgtg tatttagata aagatagtct
    38521 tttgaaacta ttgccatatc aagtgtttgg tgaaggcctt ccacattatc aagcagataa
    38581 atcggctgat tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga ctttattaaa
    38641 tatgcttaga agagaagacg aacttcccat atacaatcaa acacttgcct ttcaaaacca
    38701 cagtcagctg ctactacatg attttttgac aaggcctgag gaaatattgt cagaggaacg
    38761 tatggctgat tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat tctcactgac
    38821 gaatgatgaa ttggagaagt taatcagtaa acaaaaatta gaaaatacta gtgggctatt
    38881 tattcatccg gcgattgacg ctactttttc gtttttatat tggtacagcg atccttacaa
    38941 ccatgaccgt ttcatggacc cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa
    39001 tatgttagtc ttattgcaag aagaaaacgg agaaagtagt gatagcagtg atcgttaacg
    39061 aagagctgtt tcaactggag gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta
    39121 aaacgatggc ttcttatctt gaacataaac agtcaatgga agaatctgca aaagtttgcc
    39181 agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg gcatatggtg
    39241 agtatgcacc tgattatcgt gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg
    39301 atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa agtctgttgg
    39361 atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta gatgcaggta
    39421 gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat gcaattagac
    39481 aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa
    39541 caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag aatgaaatat
    39601 gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat ccgtaaatta
    39661 cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa ttttgccgaa
    39721 aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga gctaaataca
    39781 gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta aataaaaaat
    39841 aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta tactagtacc
    39901 agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg gcgggcgccg
    39961 tttaaaaagt ttagcagggg cgaacaccag accaactata gataaagtaa aagaatccat
    40021 attcaatatg attggaccat attttgatgg agagacagta ttggatctgt ttgcaggaag
    40081 cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat gcgtagacaa
    40141 aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag aaccagagaa
    40201 atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag cggagaaaca
    40261 acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat
    40321 ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat gcgaaacgga
    40381 taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa aacagattta
    40441 tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg tgcattattc
    40501 ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg tgcggctaaa
    40561 ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag tttgtttccc
    40621 ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc
    40681 atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc agatgcgttg
    40741 atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc tcagatgaat
    40801 catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga atatagtcac
    40861 gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag cagctactta
    40921 ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag ctaaacaatg
    40981 gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg tagtagttcc
    41041 cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac taattacggt
    41101 caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg gtatcaggca
    41161 agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat tgatcagtga
    41221 agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac agtattatat
    41281 ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca agccttataa
    41341 aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt tctttggcaa
    41401 gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa gtacagaagc
    41461 atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca gttatttgca
    41521 taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag ataaaaaggc
    41581 aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag atgttcgtat
    41641 taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag aaatctatga
    41701 acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa
    41761 cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa gtgcggataa
    41821 agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga
    41881 atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa aattgaattc
    41941 ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc tagagaaaat
    42001 gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca acccctcttt
    42061 ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg taattagatg
    42121 ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa aggagtaatg
    42181 aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa
    42241 atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat cttttgtatc
    42301 gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga gactgctgca
    42361 aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga aggaaacgat
    42421 gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag
    42481 cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt
    42541 ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat agaagatcag
    42601 caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga tcagttggca
    42661 tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa catcaataca
    42721 gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc gcaagaaatc
    42781 attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca ggagatttct
    42841 ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa atagaatgat
    42901 tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa taatccaatt
    42961 ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct tgattgttcg
    43021 caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa tatctgggat
    43081 tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag aacgctttgc
    43141 caaagatgta cttagctata cagcagataa agtatttatt ctcattggga cgaatgatct
    43201 agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt
    43261 cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc agcagtcgat
    43321 gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc tgttctcaca
    43381 gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt cttgaacaaa
    43441 tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc ggcagtgaag
    43501 ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt tgtttaagca
    43561 atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct
    43621 tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa tgaacgcatt
    43681 ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag tacgtgtaca
    43741 cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg cggctataat
    43801 ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt ggatggacac
    43861 tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa acttggtatc
    43921 ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg tacaaaggct
    43981 ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa tgatccttat
    44041 gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt agactcacag
    44101 catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt tagaaaggca
    44161 tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt tgttgtatta
    44221 cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt tttcgctaaa
    44281 agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat ccatttctta
    44341 taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt
    44401 tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt tatcagagaa
    44461 taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga aaacatggct
    44521 taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct ctccagcaaa
    44581 aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata gaatttctgg
    44641 acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc gaaactggct
    44701 tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc aaaaaacaag
    44761 tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag aaccagaaga
    44821 aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac acatccaatt
    44881 ttatttaggg tggctgtatt atttatttcg acgtatcgga tggccactac acgtcagtat
    44941 tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt ctatttctgt
    45001 tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga ataagcatta
    45061 ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg atcctcgatt
    45121 tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta tcctgatttc
    45181 ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat
    45241 gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt ttacattact
    45301 gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca cactcttcaa
    45361 tcgctggctg ccggaaactt tacttttgct gatagaagag ggattaatct tatttgagcg
    45421 aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc ttgtgctatc
    45481 tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac aacctgtgaa
    45541 acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat ttttacgctt
    45601 ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc
    45661 ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg aaagagaaaa
    45721 gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat atctaaaagg
    45781 tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg atcatgtggg
    45841 agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg gttggggcgc
    45901 tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg gtactgagat
    45961 cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg tattgactcc
    46021 ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg aatataacaa
    46081 taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat
    46141 ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc gtacgtcaag
    46201 taacacagat tttctttccc atattgaagc aaaacatggg attatatcct gcggcgtaaa
    46261 taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa atcagataaa
    46321 aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag tttttcatcc
    46381 tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg gtaggataat
    46441 ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa
    46501 aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat caagttcgga
    46561 cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt ttaagttttg
    46621 acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg ccgtttttcg
    46681 gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa aaagtaaaca
    46741 atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca ttagaatcga
    46801 ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa gtaacgaaac
    46861 aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc
    46921 aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt gcaattgatt
    46981 tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta cagaaattgg
    47041 tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa ttagttccta
    47101 aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg aagacggaac
    47161 aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc aaaattaatg
    47221 cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta aagattggtt
    47281 atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta aaacttgcca
    47341 tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat caattagttg
    47401 agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt caactattcg
    47461 ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg caaagcatgc
    47521 gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc gaagccgatt
    47581 cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt tgtttttgtg
    47641 gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat ataacgcatc
    47701 tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag tacgcatagc
    47761 gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac gtttgattgc
    47821 agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg cgtgttaccg
    47881 cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca
    47941 agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg gatagtacaa
    48001 gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc aaactagatt
    48061 tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt tattatataa
    48121 caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg ttgttctttg
    48181 tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca gtaaaggcta
    48241 ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca ttttttttat
    48301 gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt tatttagggg
    48361 caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga tcacacggtc
    48421 ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc
    48481 aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa
    48541 aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca ttctttcttt
    48601 tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat
    48661 cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat tgattaaaaa
    48721 aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc aaaaagcagt
    48781 atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca gaatcaaaag
    48841 taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt cgaacattgt
    48901 atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga atctcctaaa
    48961 atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag taagtgatac
    49021 cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc
    49081 cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca cccttggaag
    49141 ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg gatttaaagg
    49201 cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga atagccgctg
    49261 tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa aaggaacggg
    49321 aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga ctttgatttt
    49381 tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc atgaaatata
    49441 ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa agtaggaatt
    49501 catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa tcataaaaaa
    49561 acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag acttacattt
    49621 ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat tcagaataac
    49681 caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa attgcaggaa
    49741 gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt gtttgatgat
    49801 ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt cgcttttcat
    49861 taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag cgacacctaa
    49921 agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg catttacttg
    49981 gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac agcttgcata
    50041 gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat ggtcgccggt
    50101 tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt gggtatttgc
    50161 ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag aaagataaat
    50221 cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt tgctgattat
    50281 catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta aataggtagt
    50341 ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt caggtattgt
    50401 gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt gttccatagc
    50461 aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag ttacaactgg
    50521 ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt cagaaagctt
    50581 ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg aaagagcagc
    50641 tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa ctaataatac
    50701 cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg ggagaaggat
    50761 cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc gttcgataaa
    50821 tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa tcttagcaaa
    50881 gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt gattcagcgt
    50941 tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac cgcttaatgc
    51001 agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa cagctgctcc
    51061 tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt cttgcatttg
    51121 tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag cagtaatttc
    51181 tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa agatgaatgt
    51241 caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag ctaaagccat
    51301 caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt ccgatgtttg
    51361 tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg taagagagaa
    51421 tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg ctgcaaaagc
    51481 ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata gcataataaa
    51541 aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat ttggtgatta
    51601 agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa aaaaccagaa
    51661 catttctcaa ataagagaag tgttctggtt ttttctacat tgcaataaac caataaccaa
    51721 tcaaaatgat accaaggata atacgatacc agccaaaagc ggtaaagtcg tttttcttca
    51781 aatagcttag taagaatttg atagctaaga cggaaaccac aaaagatacg aatgttccta
    51841 caagaagaac aacagtttct tccattttga atgaattccc ttcagctaaa tatttgacga
    51901 tcttcaagcc gcttgcgcca aacatgacag gaatccctaa gaaaaatgaa aactctgtag
    51961 cgacaaaacg cgaagcacca atcaagatag atcctaggat cgttgcgcca gaacgggatg
    52021 ttccgggaac aagtgccaag acttggaata acccaacaag aagagctgct tggtacgtaa
    52081 agtcattcag agaagtccaa cgaggtgttt tgtttttgtt gcgtttttcg acaataataa
    52141 aggcaatacc gtaaacgatc aaaacaatgg caactggtaa gaacttgtgg aaatttgctt
    52201 ctaaccaatc atccaaaggc aaaccaatga tgatggctgg tatgcaagca acgatcactt
    52261 taaaccaaag tgtccacgta tctttttttt ctaccgttga tttacttgga gaaaatgggt
    52321 ttaacttgtg aaaataaaga acgacgacag ccaagatcgc ccctaattgg ataacgacat
    52381 tgaacatcga cataaattgt gaactggcat ccagtttgat gaattcatct gctaggatca
    52441 aatgaccagt actactgatg ggcagccatt ccgttattcc ttcaataata ccgagaatca
    52501 ctgctttgat aatatttgca aaaaacataa attccttcct tcccaattaa gatacatctt
    52561 atagtatacc gttatttttt gagaaaccca atctgttttt taatattaag aaaaacttga
    52621 gaaataaagc agataagtac acgaagagaa aaaagaagac aaagccctcg ttcagacttt
    52681 gccttttatg attctctggt aaaaattaaa atatccccat tatagaaacg agtcgcgact
    52741 atgtgttttc ccgttatcga tataagacgt atcattgatg ccgtggatag aaaatcgacc
    52801 gtttgcataa gtgacctttg ttacgctgcc atttaaaagc ccagcacgaa caggctgttt
    52861 aggatcgatc agattaagca aaaaggcgat cgtcaaacca tgagatacga ctaaaacatt
    52921 tccaccacca tttttttcgc agtgatgggc gatatcttca aatcctgtcc atacacggtc
    52981 tcgcaatgtt tcataagttt ccgcccaccc agcggtatct aagttgtata cagcattagc
    53041 aatttgttca aaagaaactt ctgtggtaaa catttcatca tatgttttaa aattcaatac
    53101 acgagggatc acaccccaca tttccatgtc atatccacct tcaagagacc cgaaacacca
    53161 ttctctaatc cttttttcaa taaaataagg aatttctttt cccttagaat gttctcctag
    53221 aataatacgg gcagtttcga ttgcacgacc actgtcacta gaataagcaa atgaaaagtc
    53281 gacatttttt aacccgcgac ctaaataatg aatgccttct tgtccttgct ttgtgagggg
    53341 tgtatcacac cagccttgta ctcgttcaat tgtattgaac atcgtttttc cgtgtcgtac
    53401 gatataaagt gtagtttctg tcattttatt ctcctcctaa gtcgttttaa cgattgaaaa
    53461 aaggatttgt ccttttttcc tgttcaatcg tcgttgcatc gccatgtcct ggatagactg
    53521 caaattcatc aggtaataca aacagctggg tccgaatact atgaagtaat tgttctaaat
    53581 tccctgtata cagatcggtt ctaccgatac ttcctctaaa cagggcgtcg ccagaaacga
    53641 caaaatcatc aaaaataaag ctgacgcttc ctatagaatg ccctggggta gggacgacgc
    53701 gaaaagacat gtttcctaaa gtataagttt tcatttcaaa ttcatattct gcaggcgaga
    53761 caataacatc agcaatatca tcatgtctac cgagtccaga aagattcaaa atcggatcac
    53821 ctagccattt ttgctctagt gggctgacat aaactgggat ttgataatac gcgcgtaaag
    53881 gttccactgc accgatatga tcgtagtgag tatgggtaag caaaatcgct accggctgtt
    53941 ggtttgtttt tttgatttct tgtttgattc gttccgcatc ttctccaggg tcaatgatca
    54001 aaagtgcttc ttcgttgtag accaaataac agttttcttc aatcattcct gtttttaatc
    54061 tttcgatatg catgtgcatt cctccttgat agccaaaact tctttcatcc attagtataa
    54121 cgcattcatt gctaggaaac aaaaacaggt acttatttct ctttacttat ctgaattatc
    54181 agactatatg aaaagtattt ggatattcta aagagaagga aagtaagttt tcatagcatt
    54241 ttaatatgtc cttgttacat tagattcatc caaaaatcca aattctatat cctatatccc
    54301 tgcctaatca gcagggtgtt tttttatgtt tggaaagaga gctctttttg ctaaactagg
    54361 ttttttcagg taatcttaga gagaaagagg tggtggaaat gatcaaacag tcaaaaatat
    54421 ctgtgcggca cttagtagaa tttattttgc gacgtggaag tatcgacaat cgtaaaaaaa
    54481 gtaaccatac tgcattagaa ggagcgagaa tccaccgcaa gctccaaaaa gatgcgggag
    54541 aaaactatga aaaagaagtc tttttaaaaa caacagttca attagatgaa tattacctga
    54601 tcgttgaagg ccgtgcagac ggtattttca aaagagacgg tatttattat atagatgaaa
    54661 tcaaaacctc cgaaccacga tttgaagatt tagaaccaga acaagtggaa cttttctttc
    54721 atcaagcaag ggtgtatgcc tatatctata gtcaagaaca tgatttacaa gaaataaatc
    54781 tgcaattgac ttacttccag acggcagaag aagtgatcac gagaaaggtt gaacatcaga
    54841 caagagagca gttagaaact ttttttaaaa agctgactga agattataaa gaatggcttg
    54901 tttttcaaga aaactggcgc actgtacgca atgcctcttt gatggctttg aaattccccc
    54961 atgatgagta taggaaaggt cagcgtgaac tagcggtagc tgcttataaa acgatccgta
    55021 cgaaacagaa attgtttgta gaagcaccta ccggaacagg aaaaacaatc tctacgttat
    55081 tccccgcttt gaaagctgta ggggaagaag agggagaaaa gatcttttat ctaacggcta
    55141 aaacaataac tagacaagta gctgaagatg ctatgacagc attaaaagat acaggagctg
    55201 aagtaaaaag tgtcactttg acggcgaaag acaagatctg ctttttagat gagacgacct
    55261 gtaatccaga tcagtgccca tatgccaatg gttattacaa tcggattaat gaaggtttat
    55321 gggatctttt gaatcatgag aatcagatca ctcgtgaagt catcgaaact tatgcaagaa
    55381 agcatacact ttgtcctttc gaactatcat tagatgtcag tatctggtgt gacgtaatca
    55441 ttggggatta caattattta tttgatccaa ctgtttattt aagacgtttt tttgaagatg
    55501 aaaaaaacga ggactatctg tttttgattg acgaagcaca taatttggtg aatcgttccc
    55561 gagagatgta ttcggcagaa ctatcttatg aaaaaacgaa acgaagtaag gaagctatac
    55621 caaaggaatt caaaaaactt catcgccgtt tcaacaaact attaaaagaa tttgattcga
    55681 tccgagaaat agcaaaagaa gatcattggg attatcatca tcaaaaagca ccggctgaat
    55741 ctttagtaaa ggcaggttat caattaagcg aaaaaattaa agaatggctg gcagaatttc
    55801 ctgagcaccc atcacaagaa caattgcttc cttattattt tgatttgctt cattttttga
    55861 aggtcagtga attttatgat gatcactacg aaacaacagt cgaaaaaacg tatcgcgatc
    55921 tgatagtaaa agagttttgt attgatccaa gcttgtttct tgagcagtcg ttggataaag
    55981 gaaaaagcag tattcttttt tctgctagtt tttcaccact ctcttattat caggaaacac
    56041 tggggggaca aaaaagtctg gcttacaaac tgccgagtcc tttcccagaa ggaaaccagc
    56101 aagttttcat cgcaaattac ctagagacga cttatcgaaa aagggaacac agtctggcaa
    56161 aattagtaga gacgattcat gcttttgctg atggaaaggt cgggaattat ttcgttttct
    56221 ttccttctta tcaatattta gatcttgcgg tggaagcatt cagacagcaa tatcccgaca
    56281 gcaatgtgct aatccaagaa acggacatga atgaggaaga acgcgaacga tttttaacaa
    56341 aatttcaaac aaatcccgct gaaacattga ttggattttg cgtacttggc gggatttttt
    56401 cagaaggaat cgatttgaga ggggaccgat tgatcggcag tatgatcgta ggtgtaggtt
    56461 tgccgcagat gaatcatgag caggaactga tcaaatcata ttatgatgag aaggaacacc
    56521 ttggatttgc ttacgcttat cagcttcctg gaatgaacaa agtcttgcaa gctggtggtc
    56581 gagtgattcg tggcatggcg gatcaaggaa tcgttgtatt ggcagatcaa cgattttcta
    56641 gtttttctta tcgacgttta ttcccacaac attggcaaac agcaagagaa gtacgaagtg
    56701 ttggggaatt ggaagaagga atacagcgtt tttggttatc aaaagaagca gtaaatcaaa
    56761 aaaacgagga atgacagacg tcagcctcgt tttttgatta acgatagtta gtgaattgca
    56821 actcgattgg taagtcaagt ttacgtaata aagcaatcac agcttgtaga tcatcacggt
    56881 tcttacctgt cactctaatc tgatcttctt ggatttgtgc ttttactttc agtttttcat
    56941 ttttgatagc tgtagtgatt ttcttcgcgt tttcacgatc gattccgttg accaaatcgg
    57001 cagtttgtcg agctttgcca cctagagcgt gttctgattc ggaaaaatgg atatttttga
    57061 ttggcacatt tcttttgatc agcttaccaa ataatacatc tttgatctgt tcgattttga
    57121 aatcatcatc gctcaataca actagattgc tgttctctaa tttgatttct actgtagaat
    57181 ccttgaagtc aaagcgattg gacaattctt ttgttgtttg ctgaatcgca ttttttactt
    57241 cttctgtatt gacttctgat acaatgtcga agctagcatc ttttgccata ggaaccctcc
    57301 tgatatacat ataaatattt accatacatc tatcttatca taggattagg gcagattctt
    57361 acctgtttgc taaaattctt ctcatttgag ttcgaagtat tttattttaa tagataaact
    57421 gttattctta catttggaat accaaagtag gggagaggtg cttgtgagta aaattatgac
    57481 tgcgtatcat aaaaatgaaa gattaaagaa aatagtggtt attttgataa ccggattgac
    57541 cgctgcggtc gctttaaatt attttttgat tccagccaat gtcttttctg cagggatgaa
    57601 tgggatcgct caaatcattt cctcgctttt atatgaatgg tttcatatca agatagacac
    57661 aggaacattt atttttcttt tgaatatccc tgtgtttgta ttagggtttc tcaaagtagg
    57721 aaaacgagca acgatcctta gttttgtgaa tgttgtatgt gtatcggtga tgacgatgct
    57781 gcttccagtc ggacagatca ctgataatat tttgatgaat gccctagtag gcggtgtttt
    57841 attaggtctt ggcgtaggat tatctttaaa aatgggcttt acaactggtg ggatggatat
    57901 catctctctt gtactttcac aaacaactgg aaaaacagtc ggaaactaca tgctgattct
    57961 aaatggtatc attgtagtca tcgcaggctt cttctttaac tgggaaagtg ccttgtatac
    58021 gattatttcc atttatgcaa tgactcaagt aattgatgcc atccacacga gtcatcaaaa
    58081 agtaacagca atgatcgtta ccgtcaagcc agaagaggta acagaagaac tagcaaaacg
    58141 gatggtacgt ggaatgacat tactgccgtc tgtcggtggc tattcaaaaa aacaagggaa
    58201 catgatcatg atggtcatca cccgctacga aatgtatgat cttgatcaga tcgttcatga
    58261 aatcgacgac gatgcattta tcaatatttt accaactcat tcagtatttg gccggtttgc
    58321 aaatgaaaac gaacaaaaaa tgtatcgaac aactggcgta ttgccagaaa taaaaaatcg
    58381 ttcacaaaaa aaatgaggaa tcaggtttat tttagaataa atttaatcta ttacatctaa
    58441 taagaacatt tttataaatt taaacgagaa tcttacttct aaattctcat ttacaagcag
    58501 ctaaaaaaag agtatgataa ataaaaatcg tgttaaggag tgtcgtacga tgagcgaagc
    58561 aaaccaagaa cggtctggac aagaaataga actaatcaaa gaacgtattc ttgctgcctt
    58621 ggaaatggtg attgatcctg aattaggaat cgacatcgtc aatttaggct tgatttatga
    58681 tatagaattc aatcctgaaa atggagaaac agtcattaag atgactctaa caactatggg
    58741 ctgtccatta gcagatattt taacagaatc tatccatgac gcattaaaag aagtgccaga
    58801 agtaacgaaa gcagaagtca aactagtctg gtatccagca tggacgacag ataagatgag
    58861 ccgttatgca cggattgcgt tggggatacg atagaaatct tggctgacta gctatgagaa
    58921 gcaaatagga aaaacaaaaa agccttttat cctttctaga agaaaagaga taaaaggttt
    58981 ctttgtttat aaaattttgt ctgctatatt ataagttaat taaataaatg tgttctattg
    59041 ataaaggaca ttcaaatccc tttccttttg gaatccttct tttttacatc agtttgattt
    59101 tcgtatagta ttctttctca tcttcacgat aaatcgcagc cccatttttt tgttgtgctt
    59161 taagactgcc aagatttttt ttatttacag acaaaagtgc ctcatcaatg ccgatttttt
    59221 ttgcctcttg tagtactaat gctaatcctt gtgtggcata tcctttttta cgataattag
    59281 gggagatgcc gtaaccaata tggccagcgc cgtttcttaa tgtttcgttc agtttatggc
    59341 gcaaattaaa aattcctaca tactcatgtt catcactttc taaaataaag acggttgcgg
    59401 gaacgtaact ctcaggaaga tcgattccta aagacgtttt ttcgcatttt tttacataat
    59461 ctaaaaattc agcaaatgta tagccataag cattgttgat aaatccactc tcatttggtc
    59521 caaagctagt ttgtagctga taagctttct ttaaatctgc ttcccatagt ttgatcagtt
    59581 tcatcgtatt cacctcttta agtaacctta ttcatttgtt ttctactata gcactattgt
    59641 tgtaaatagc catgcctatt tttatttatt gtgtcatatc gtattaagaa tcatcgaaca
    59701 atacaaatat gaattacaat aaaaaaatta tttgaagttc gttcattcgt tacttatgat
    59761 ctctagaaga aagaaaacgt gaattacttc agcgtctaga attccagaag gatagtaatc
    59821 aacagaagaa ataatcaata tccaaatgaa agaagatagc tgaatcgaga aaaagaggaa
    59881 aaaagaaatg aagaaaaaaa taaaatagtt actaatccca gagtcgttcg tttaacattg
    59941 aaatcgatca tatggataaa acagattttt tcaccaaccc tcaatagatg atggagttgc
    60001 tcggatgaat tatttgtgta gatggcataa gggagggcgt acagaaatga aaaagaacaa
    60061 aaatatgttc ttgacaaaaa acacatgtaa tcctaaacta aaagatgtga tgcggctatg
    60121 gcggaattgg cagacgcgct agcttcaggc gctagtgagg gtgacctcgt ggaagttcga
    60181 gtcttcttag ccgcataatt tgttttttta taaagagaag gtacacctgg agcgatgaga
    60241 aagaaaataa catattatct aaaagaatat aggaaaaatt ttaaacaacg gaaccagatt
    60301 caaataaatc aaaaaaaatt tgtgtgaagg tgggacagtt taaaacctaa tagattgatt
    60361 caaatagtag atttatagat tctgatacaa gaaatactgg caaacgatag ttaaggaata
    60421 aagactaaac ttaaactcaa aaagaggtca tgcgaaagaa taaatcgcat gacctcttac
    60481 tatattttga atgtgtttaa acgttcaaat aatgaatctt agtaccaacc gtttgcttcc
    60541 cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact
    60601 ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgg
    60661 ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat
    60721 tcttttgcag agcttgtact tgctggagta gcagcagttt ctgttacagc aggagcaggt
    60781 tgtgtttcag caacaggcgc tgcttgtgtt tccgtttgaa ccgtttcttg tacaacaggt
    60841 tgttcagctt gaacaggttc agcttggact ggtgcttctt gaacggctgc ttgaggttca
    60901 gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAXWLB010000002        56001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXWLB010000002 DAXWLB010000000
VERSION     DAXWLB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.002"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            43..1674
                     /locus_tag="ACGE4H_000058"
     CDS             43..1674
                     /locus_tag="ACGE4H_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFF8363511.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1736..2377)
                     /locus_tag="ACGE4H_000059"
     CDS             complement(1736..2377)
                     /locus_tag="ACGE4H_000059"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8363512.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVPPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2563..2639
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2659..2967
                     /gene="rplU"
                     /locus_tag="ACGE4H_000060"
     CDS             2659..2967
                     /gene="rplU"
                     /locus_tag="ACGE4H_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFF8363513.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2994..3338
                     /locus_tag="ACGE4H_000061"
     CDS             2994..3338
                     /locus_tag="ACGE4H_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFF8363514.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3351..3644
                     /gene="rpmA"
                     /locus_tag="ACGE4H_000062"
     CDS             3351..3644
                     /gene="rpmA"
                     /locus_tag="ACGE4H_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFF8363515.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4078..6885
                     /locus_tag="ACGE4H_000063"
     CDS             4078..6885
                     /locus_tag="ACGE4H_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8363516.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7076..7567
                     /locus_tag="ACGE4H_000064"
     CDS             7076..7567
                     /locus_tag="ACGE4H_000064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8363517.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7670..8659
                     /locus_tag="ACGE4H_000065"
     CDS             7670..8659
                     /locus_tag="ACGE4H_000065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFF8363518.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8692..9495
                     /locus_tag="ACGE4H_000066"
     CDS             8692..9495
                     /locus_tag="ACGE4H_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFF8363519.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9516..10430
                     /locus_tag="ACGE4H_000067"
     CDS             9516..10430
                     /locus_tag="ACGE4H_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8363520.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10513..10887
                     /locus_tag="ACGE4H_000068"
     CDS             10513..10887
                     /locus_tag="ACGE4H_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFF8363521.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11026..11271
                     /locus_tag="ACGE4H_000069"
     CDS             11026..11271
                     /locus_tag="ACGE4H_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8363522.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11443..12507
                     /locus_tag="ACGE4H_000070"
     CDS             11443..12507
                     /locus_tag="ACGE4H_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFF8363523.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12753..13178
                     /locus_tag="ACGE4H_000071"
     CDS             12753..13178
                     /locus_tag="ACGE4H_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8363524.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13171..13620
                     /gene="nusB"
                     /locus_tag="ACGE4H_000072"
     CDS             13171..13620
                     /gene="nusB"
                     /locus_tag="ACGE4H_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFF8363525.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13717..14571
                     /locus_tag="ACGE4H_000073"
     CDS             13717..14571
                     /locus_tag="ACGE4H_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFF8363526.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14572..15909
                     /gene="xseA"
                     /locus_tag="ACGE4H_000074"
     CDS             14572..15909
                     /gene="xseA"
                     /locus_tag="ACGE4H_000074"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFF8363527.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15914..16138
                     /locus_tag="ACGE4H_000075"
     CDS             15914..16138
                     /locus_tag="ACGE4H_000075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFF8363528.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16140..17030
                     /locus_tag="ACGE4H_000076"
     CDS             16140..17030
                     /locus_tag="ACGE4H_000076"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8363529.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17039..17857
                     /locus_tag="ACGE4H_000077"
     CDS             17039..17857
                     /locus_tag="ACGE4H_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFF8363530.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVASNAHEQFDHK"
     gene            17964..18413
                     /locus_tag="ACGE4H_000078"
     CDS             17964..18413
                     /locus_tag="ACGE4H_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8363531.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18431..20113
                     /gene="recN"
                     /locus_tag="ACGE4H_000079"
     CDS             18431..20113
                     /gene="recN"
                     /locus_tag="ACGE4H_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFF8363532.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20168..20284)
                     /locus_tag="ACGE4H_000080"
     CDS             complement(20168..20284)
                     /locus_tag="ACGE4H_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFF8363533.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20357..21313)
                     /locus_tag="ACGE4H_000081"
     CDS             complement(20357..21313)
                     /locus_tag="ACGE4H_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8363534.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21484..21852
                     /locus_tag="ACGE4H_000082"
     CDS             21484..21852
                     /locus_tag="ACGE4H_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFF8363535.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            complement(22055..23368)
                     /locus_tag="ACGE4H_000083"
     CDS             complement(22055..23368)
                     /locus_tag="ACGE4H_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFF8363536.1"
                     /translation="MATLHEKKVQFNSKLTISNTGGNLSTDSGLVLVKEFMESLNFSD
                     LSKQYLGIEDKRLYHIHDNFSLMEQLIYQNIAGYSTDSSANILKQDPIFKVILDKSRL
                     ASQSSISRFWDRISEENISQLQDLNQAMIDKVRSARNTTEMIFDLDSTHSDTYGDQEN
                     TDYNAHYRTNGYHPLVAFDGLTGDFLKAELRSGNVYTSNGVGTFMEPLFEHYNQVVPV
                     SNILVRGDSGFATPELYDLCEAYDSFFVIRLKANRNLSKIAESFIQIDDNHPWDKKEV
                     VYSSTSYQAKSWSKERRVCIKSTREADELLFRHEYIVTNYSNNVSAETVFRTYCKRGT
                     MENFIKEAKNGFYFDKTDSPSFLENHARMMVSLLAYNIVNFMRTLCFTQGSASMQVDT
                     IRLKLFKVAGKLVRTGRRLLLKLSSYHLHQGLFYQVLGNIQQLCW"
     gene            23686..24117
                     /gene="mraZ"
                     /locus_tag="ACGE4H_000084"
     CDS             23686..24117
                     /gene="mraZ"
                     /locus_tag="ACGE4H_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFF8363537.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24138..25097
                     /gene="rsmH"
                     /locus_tag="ACGE4H_000085"
     CDS             24138..25097
                     /gene="rsmH"
                     /locus_tag="ACGE4H_000085"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFF8363538.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25102..25503
                     /gene="ftsL"
                     /locus_tag="ACGE4H_000086"
     CDS             25102..25503
                     /gene="ftsL"
                     /locus_tag="ACGE4H_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8363539.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            25504..27696
                     /locus_tag="ACGE4H_000087"
     CDS             25504..27696
                     /locus_tag="ACGE4H_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8363540.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            27722..28684
                     /gene="mraY"
                     /locus_tag="ACGE4H_000088"
     CDS             27722..28684
                     /gene="mraY"
                     /locus_tag="ACGE4H_000088"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFF8363541.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            28698..30059
                     /gene="murD"
                     /locus_tag="ACGE4H_000089"
     CDS             28698..30059
                     /gene="murD"
                     /locus_tag="ACGE4H_000089"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFF8363542.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30085..31173
                     /gene="murG"
                     /locus_tag="ACGE4H_000090"
     CDS             30085..31173
                     /gene="murG"
                     /locus_tag="ACGE4H_000090"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFF8363543.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31188..32408
                     /locus_tag="ACGE4H_000091"
     CDS             31188..32408
                     /locus_tag="ACGE4H_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFF8363544.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            32564..33889
                     /gene="ftsA"
                     /locus_tag="ACGE4H_000092"
     CDS             32564..33889
                     /gene="ftsA"
                     /locus_tag="ACGE4H_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFF8363545.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            33912..35153
                     /gene="ftsZ"
                     /locus_tag="ACGE4H_000093"
     CDS             33912..35153
                     /gene="ftsZ"
                     /locus_tag="ACGE4H_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFF8363546.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35170..35847
                     /locus_tag="ACGE4H_000094"
     CDS             35170..35847
                     /locus_tag="ACGE4H_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFF8363547.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            35861..36460
                     /locus_tag="ACGE4H_000095"
     CDS             35861..36460
                     /locus_tag="ACGE4H_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFF8363548.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            36541..36762
                     /locus_tag="ACGE4H_000096"
     CDS             36541..36762
                     /locus_tag="ACGE4H_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFF8363549.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            36811..37593
                     /locus_tag="ACGE4H_000097"
     CDS             36811..37593
                     /locus_tag="ACGE4H_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFF8363550.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            37614..38321
                     /locus_tag="ACGE4H_000098"
     CDS             37614..38321
                     /locus_tag="ACGE4H_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFF8363551.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            38583..41381
                     /gene="ileS"
                     /locus_tag="ACGE4H_000099"
     CDS             38583..41381
                     /gene="ileS"
                     /locus_tag="ACGE4H_000099"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFF8363552.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(41430..42956)
                     /gene="zwf"
                     /locus_tag="ACGE4H_000100"
     CDS             complement(41430..42956)
                     /gene="zwf"
                     /locus_tag="ACGE4H_000100"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFF8363553.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(42971..43618)
                     /locus_tag="ACGE4H_000101"
     CDS             complement(42971..43618)
                     /locus_tag="ACGE4H_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8363554.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(43802..44131)
                     /locus_tag="ACGE4H_000102"
     CDS             complement(43802..44131)
                     /locus_tag="ACGE4H_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFF8363555.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44308..45036
                     /gene="sfsA"
                     /locus_tag="ACGE4H_000103"
     CDS             44308..45036
                     /gene="sfsA"
                     /locus_tag="ACGE4H_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFF8363556.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45052..46065
                     /locus_tag="ACGE4H_000104"
     CDS             45052..46065
                     /locus_tag="ACGE4H_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8363557.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46065..47342
                     /locus_tag="ACGE4H_000105"
     CDS             46065..47342
                     /locus_tag="ACGE4H_000105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8363558.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47405..50107
                     /locus_tag="ACGE4H_000106"
     CDS             47405..50107
                     /locus_tag="ACGE4H_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8363559.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50259..50576
                     /locus_tag="ACGE4H_000107"
     CDS             50259..50576
                     /locus_tag="ACGE4H_000107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8363560.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            50606..50926
                     /locus_tag="ACGE4H_000108"
     CDS             50606..50926
                     /locus_tag="ACGE4H_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8363561.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51034..52494
                     /locus_tag="ACGE4H_000109"
     CDS             51034..52494
                     /locus_tag="ACGE4H_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8363562.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            52562..52783
                     /locus_tag="ACGE4H_000110"
     CDS             52562..52783
                     /locus_tag="ACGE4H_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363563.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            52814..52996
                     /locus_tag="ACGE4H_000111"
     CDS             52814..52996
                     /locus_tag="ACGE4H_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFF8363564.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            52996..53409
                     /locus_tag="ACGE4H_000112"
     CDS             52996..53409
                     /locus_tag="ACGE4H_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFF8363565.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            53532..54713
                     /locus_tag="ACGE4H_000113"
     CDS             53532..54713
                     /locus_tag="ACGE4H_000113"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8363566.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(54784..54957)
                     /locus_tag="ACGE4H_000114"
     CDS             complement(54784..54957)
                     /locus_tag="ACGE4H_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363567.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTPKTKE
                     NKKFKFTKKEDLL"
     gene            55111..55184
                     /locus_tag="ACGE4H_000115"
     tRNA            55111..55184
                     /locus_tag="ACGE4H_000115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55145..55147,aa:Arg,seq:cct)
     gene            complement(55253..>56001)
                     /locus_tag="ACGE4H_000116"
     CDS             complement(55253..>56001)
                     /locus_tag="ACGE4H_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8363568.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTM
                     STYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18400 a         9537 c        12055 g        16009 t
ORIGIN      
        1 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
       61 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
      121 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
      181 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
      241 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
      301 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
      361 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
      421 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
      481 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
      541 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
      601 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
      661 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
      721 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
      781 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
      841 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
      901 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
      961 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     1021 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     1081 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     1141 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     1201 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     1261 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     1321 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     1381 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     1441 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     1501 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     1561 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     1621 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     1681 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     1741 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     1801 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     1861 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     1921 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     1981 tcgcaattaa ttttggcggg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     2041 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     2101 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     2161 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     2221 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     2281 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     2341 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     2401 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     2461 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     2521 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     2581 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     2641 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     2701 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     2761 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     2821 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     2881 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     2941 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     3001 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     3061 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     3121 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     3181 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     3241 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     3301 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     3361 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     3421 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     3481 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     3541 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     3601 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     3661 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     3721 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     3781 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     3841 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     3901 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     3961 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     4021 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     4081 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     4141 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     4201 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     4261 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     4321 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     4381 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     4441 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     4501 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     4561 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     4621 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     4681 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     4741 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     4801 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     4861 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     4921 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     4981 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     5041 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     5101 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     5161 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     5221 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     5281 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     5341 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     5401 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     5461 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     5521 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     5581 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     5641 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     5701 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     5761 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     5821 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     5881 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     5941 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     6001 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     6061 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     6121 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     6181 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     6241 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     6301 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     6361 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     6421 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     6481 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     6541 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     6601 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     6661 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     6721 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     6781 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     6841 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     6901 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     6961 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     7021 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     7081 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     7141 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     7201 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     7261 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     7321 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     7381 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     7441 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     7501 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     7561 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     7621 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     7681 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     7741 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
     7801 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
     7861 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
     7921 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
     7981 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
     8041 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
     8101 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
     8161 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
     8221 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
     8281 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
     8341 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
     8401 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
     8461 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
     8521 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
     8581 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
     8641 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     8701 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     8761 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     8821 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     8881 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     8941 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     9001 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     9061 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     9121 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     9181 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     9241 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     9301 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     9361 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     9421 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     9481 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     9541 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
     9601 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
     9661 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
     9721 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
     9781 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
     9841 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
     9901 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
     9961 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    10021 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    10081 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    10141 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    10201 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    10261 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    10321 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    10381 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    10441 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    10501 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    10561 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
    10621 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    10681 ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    10741 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    10801 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    10861 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    10921 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    10981 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    11041 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    11101 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    11161 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    11221 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    11281 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    11341 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    11401 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    11461 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    11521 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    11581 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    11641 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    11701 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    11761 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    11821 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    11881 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    11941 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    12001 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    12061 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    12121 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    12181 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    12241 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    12301 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    12361 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    12421 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    12481 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    12541 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    12601 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    12661 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    12721 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    12781 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    12841 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    12901 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    12961 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    13021 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    13081 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    13141 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    13201 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    13261 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    13321 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    13381 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    13441 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    13501 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    13561 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
    13621 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
    13681 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
    13741 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
    13801 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
    13861 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
    13921 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
    13981 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
    14041 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
    14101 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
    14161 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
    14221 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
    14281 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
    14341 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
    14401 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
    14461 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
    14521 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
    14581 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
    14641 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
    14701 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
    14761 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
    14821 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
    14881 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
    14941 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
    15001 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
    15061 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
    15121 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
    15181 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
    15241 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
    15301 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
    15361 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
    15421 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
    15481 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
    15541 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
    15601 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
    15661 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
    15721 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
    15781 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
    15841 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
    15901 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
    15961 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
    16021 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
    16081 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
    16141 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
    16201 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
    16261 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
    16321 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
    16381 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
    16441 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
    16501 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
    16561 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
    16621 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
    16681 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
    16741 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
    16801 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
    16861 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
    16921 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
    16981 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
    17041 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
    17101 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
    17161 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
    17221 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
    17281 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
    17341 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
    17401 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
    17461 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
    17521 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
    17581 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
    17641 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
    17701 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
    17761 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
    17821 ggcttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
    17881 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
    17941 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
    18001 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
    18061 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
    18121 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
    18181 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
    18241 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
    18301 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
    18361 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
    18421 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
    18481 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
    18541 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
    18601 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
    18661 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    18721 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    18781 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    18841 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    18901 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    18961 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    19021 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    19081 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    19141 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    19201 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    19261 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    19321 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    19381 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    19441 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    19501 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    19561 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    19621 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    19681 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    19741 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    19801 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    19861 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    19921 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    19981 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    20041 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    20101 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    20161 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    20221 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    20281 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    20341 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    20401 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    20461 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    20521 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    20581 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    20641 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    20701 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    20761 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    20821 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    20881 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    20941 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    21001 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    21061 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    21121 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    21181 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    21241 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    21301 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    21361 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    21421 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    21481 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    21541 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    21601 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    21661 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    21721 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    21781 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    21841 tcttacctat gaaaaaaata gaaacctgaa taattcatac cgataaaaaa atgatctgtt
    21901 gctctaaatt ttaccggaat ttatattttt tattccgtct cagaacccaa cagattaaaa
    21961 aatccgaact gtttaggtaa acagctgcgg ttcagccaaa tttgggcgca ctcgtccctt
    22021 gaagtacaat ataattttta aaattgggat ttatttacca gcagagctgc tggatatttc
    22081 cgaggacttg ataaaataag ccctgatgta aatgataaga actgagtttc agcagcagtc
    22141 tacgtcctgt tcgaacgagt ttacctgcga ccttgaatag ttttaatcgg attgtgtcca
    22201 cctgcatgct ggccgatccc tgtgtgaaac aaagcgtacg cataaagtta acgatgttgt
    22261 aagccaacag gcttaccatc atgcgagcgt gattttctaa aaaggaaggg ctatcggtct
    22321 tatcgaaata aaagccattc ttcgcttctt taatgaagtt ttccatggtg ccgcgtttac
    22381 agtaagtccg aaagactgtt tccgcagaga cattattgga gtaattggtg acgatgtatt
    22441 catgtcggaa tagaagttca tcagcttccc gtgttgattt aatgcagact cggcgttctt
    22501 tggaccaact ttttgcttga taggatgttg aagaataaac aacttctttt ttatcccaag
    22561 ggtggttatc gtcaatctga ataaagcttt ccgcaatttt tgaaaggtta cggttcgcct
    22621 ttaagcggat cacaaagaag ctgtcataag cttcacagag atcgtaaagt tccggtgtag
    22681 cgaacccgct atctccacgg acgagaatat tgctgacagg aaccacttgg ttataatgct
    22741 caaaaagagg ctccataaat gtcccaacgc cattagacgt gtagacattg ccagaacgaa
    22801 gttctgcttt taagaaatct cccgtcaaac catcaaatgc gactaacgga tggtatccat
    22861 tggttcgata atgagcattg taatcagtgt tttcttgatc tccgtaagta tcagaatggg
    22921 ttgaatccaa gtcaaagatc atttctgtcg tatttcttgc cgaccgtact ttatcaatca
    22981 tggcttggtt aagatcttgc aactgagaaa tattttcttc gcttatgcga tcccagaatc
    23041 gggaaatcga agactgcgag gcaagcctgg acttatccaa aatcaccttg aatatagggt
    23101 cttgcttcaa tatatttgcg gaggaatcgg tcgaatagcc agcgatattt tgataaatca
    23161 actgctccat caaagaaaaa ttgtcatgga tatgatagag acgtttgtcc tctattccca
    23221 agtattgctt agaaagatcg gagaaattaa gagactccat aaattctttg acgagaacca
    23281 atccagaatc ggtggacaaa tttccacctg tgtttgaaat cgtcagttta gagttgaatt
    23341 gtaccttttt ttcgtgtaat gtagccattg agagagcccc tttctatggt tttagtgtgg
    23401 taacttaacc atatcagatt ggggttcttt ttgtacaccc aaagggtgct tatcgaacaa
    23461 gtgaaaaatg ctattgcagc aaggttattt gtagtttcct gaaattttat gaattattca
    23521 ggtagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt ttaaaaaaat
    23581 gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga aagtggggga
    23641 ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt gatgggtgaa
    23701 ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact tcgtgaagaa
    23761 cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg ctatccgatg
    23821 agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa aaaagacgca
    23881 cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga taaacaagga
    23941 cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg ttgcgtagtc
    24001 atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga tttttctgca
    24061 gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg actataagaa
    24121 taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg ttgaaagaaa
    24181 cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca ctcggcggtg
    24241 ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg tatgcgtttg
    24301 accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat gtcgaacaag
    24361 gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg agtgagcatg
    24421 tagataaggt agacggcatt ttgtatgatc taggggtttc ctctccccag ctagatgaag
    24481 cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg gatcagtcag
    24541 ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta gtaaagattt
    24601 tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc gaacgagtac
    24661 gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa caagtcatcc
    24721 cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa gctgtgcgga
    24781 ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca atccggttat
    24841 tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac agaattgtca
    24901 aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt cctgtcatac
    24961 ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt ccttcagagg
    25021 aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca gaaaaaataa
    25081 gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta cgaatatgat
    25141 ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc gaatcaagaa
    25201 atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt ggaaaaaatt
    25261 gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt tatgctgcgc
    25321 acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat caatgataaa
    25381 aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac tgaacggatt
    25441 aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag gaaagtgaag
    25501 taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct aaatccgatg
    25561 aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt gttcttttta
    25621 tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac ctcattagct
    25681 gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag aggaacgatt
    25741 tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat taaagcgata
    25801 ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa gaattttgat
    25861 aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc actgaatatt
    25921 ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta tgggaaaaat
    25981 atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg tgtcgctggt
    26041 ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc ttctcatttt
    26101 attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact aggtttggag
    26161 tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca aaaagataat
    26221 tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga cggacaagat
    26281 atctacacga ctttagacag cagactacaa agttatttag aaacactgat ggatcaagta
    26341 aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa aaccggagaa
    26401 atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg attgactgga
    26461 aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg atcaaccatg
    26521 aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa tgaaaccttc
    26581 caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt tggtgaaaaa
    26641 ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat ggtcatactg
    26701 gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt tggacaaagt
    26761 actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa tattgtcgac
    26821 cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat gatgaaagct
    26881 ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag caaagtggtg
    26941 gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca acctttttca
    27001 aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag cgaaaactac
    27061 ggtattgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa aaccggaaca
    27121 gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta cctgtattcg
    27181 attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac aatgaaacac
    27241 cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat gaaacgggca
    27301 atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga aaaagtatcc
    27361 gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca aaaaagtgga
    27421 ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac tgccaacggt
    27481 gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa gctgatgcct
    27541 gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct cggtattgaa
    27601 gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata tgaaaaaatc
    27661 actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga gagaaaagag
    27721 catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga caattgccgc
    27781 aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga ttcgtgagga
    27841 gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct tagtgtttct
    27901 gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc taactcctac
    27961 tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc ttgatgattt
    28021 tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat tgattggaca
    28081 gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc caggtgtatt
    28141 gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg ctctcttctg
    28201 gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat tagttgctgg
    28261 cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc atcagtatga
    28321 tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg tctacaacag
    28381 aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg gacttttagc
    28441 ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt tgatctatgt
    28501 tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag gaaaacgaat
    28561 ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg agtggaagat
    28621 tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat ggatcgtttg
    28681 gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa gtgcttgtct
    28741 taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg ggcgcacttg
    28801 tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag ctgctttctt
    28861 tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa gaattttcat
    28921 tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa gcccaagaaa
    28981 tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa tgcccaatta
    29041 ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga ctactactga
    29101 atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat ccagcaagtg
    29161 gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct agcttccaac
    29221 tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc tatgaagcac
    29281 atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac cttcaaaaaa
    29341 acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta caagaattag
    29401 cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg gaagacggtg
    29461 tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa atatctgaac
    29521 ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct gtagcaaaat
    29581 tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat ggcgtaccac
    29641 atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat tcaaaagcaa
    29701 ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag gtcgtgttat
    29761 tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca ttaaaaggaa
    29821 tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct ggaaaaaaag
    29881 caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct ttagcattag
    29941 aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc tgggatcaat
    30001 acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga ttaaaataga
    30061 aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg aacaggggga
    30121 catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc gaatgctgaa
    30181 ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga tacaggaatg
    30241 cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca taacatcaag
    30301 acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag tgattttcaa
    30361 ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta cgctgcagcc
    30421 aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt taccaataaa
    30481 tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc ttcctttttt
    30541 cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc agatactaaa
    30601 aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt cttgattttt
    30661 ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt aagtgaatat
    30721 attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga agagatccta
    30781 aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa aaatatggca
    30841 gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc gatcgcagaa
    30901 ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac gaatgatcat
    30961 cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat tgcagataat
    31021 gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga tgaagaattg
    31081 caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc aaaacgtctt
    31141 tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg agtaagaaaa
    31201 aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact ccttggcaaa
    31261 aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca aaaaaagaag
    31321 acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt caagaggaac
    31381 agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc aagcaaaaga
    31441 aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta aaaagtcaac
    31501 ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt ataccgctga
    31561 tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca gtatcaggca
    31621 ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta ggagaaaatg
    31681 tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa gcacagccaa
    31741 gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg gatgtaacag
    31801 aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg attgaaaatg
    31861 gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc ttggaagagt
    31921 tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta tcatcagaat
    31981 tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa aatctgatcc
    32041 aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt gcgaaccaaa
    32101 tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc gacatggaag
    32161 ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg ggccaatcgg
    32221 aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt gaagaggcag
    32281 aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct gattcaaatg
    32341 attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca agtttaaatg
    32401 aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca gtaatttatg
    32461 gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg gacgctagtt
    32521 gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa aaacaggaat
    32581 gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg aatatatcga
    32641 cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta accgaggcat
    32701 tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac aagcagaaga
    32761 aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca tgcttgaagt
    32821 agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca ccgatgaaga
    32881 tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac gtcaaatcgt
    32941 gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag atcctcgtgg
    33001 aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta aaacgatcat
    33061 ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa tggtgattac
    33121 tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg gtacgatcgt
    33181 cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc aattgaagtt
    33241 taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta ttgtattaaa
    33301 tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat acccagaacg
    33361 gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac cagtgaaagt
    33421 cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt tcaacaaagc
    33481 aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg tattgacagg
    33541 cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg gcgtaaatgt
    33601 aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa atgtaataag
    33661 tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa ttgctgttac
    33721 gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa acacatatga
    33781 gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag agtccggaga
    33841 aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat tcatcctggg
    33901 aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca gtaatcaaag
    33961 tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa gaaaacgtta
    34021 aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat tcaaaagctg
    34081 aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt tctcagccag
    34141 aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct ttagacggtg
    34201 cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt gctgcaccaa
    34261 tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg acacgtccat
    34321 tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct cgtttgaaag
    34381 aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt gtagataaaa
    34441 agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa ggtgtacaag
    34501 gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct gacgtgaaaa
    34561 cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt ggagaagatc
    34621 gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca tctattgatg
    34681 gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg ttcgaagcac
    34741 aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc attctaggta
    34801 catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca acaggtatcg
    34861 atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa atgcaatcac
    34921 ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca gaagaagaaa
    34981 acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga gttgatgatt
    35041 ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa gaaacaaaat
    35101 ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa taaaggagag
    35161 gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca ggattcgtgt
    35221 gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa atctgttgaa
    35281 tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga aaaccgagta
    35341 gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa gtggcattta
    35401 attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga ttactttcat
    35461 gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa agaattatcc
    35521 tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc accagatgag
    35581 cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt tgggttgatg
    35641 actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg tacgctgaaa
    35701 gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag tgaattaagt
    35761 atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt tatccgtgta
    35821 ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt taaacagagg
    35881 agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc aagattatcc
    35941 tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga atcaatcaac
    36001 tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt cacgtcctgt
    36061 aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac atcaatcacc
    36121 tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac ctaaagcaaa
    36181 tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta tggcgattgc
    36241 taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag aagaaaaaca
    36301 agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg gagatatcaa
    36361 acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg acagttcaac
    36421 cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac tacgatttat
    36481 actattgcac tattacttca tttacttcaa cgggcagcct atttctatag cattatcttg
    36541 atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct aggacgatta
    36601 ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt gaggattgga
    36661 cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt acaaggttta
    36721 gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca acaaaatctt
    36781 tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca gcactttcgg
    36841 gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt tcaaggacaa
    36901 tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt agaaacactg
    36961 attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca ggcagaaaga
    37021 aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt tgaaacagca
    37081 ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa aatccttgga
    37141 actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc ggacggtgat
    37201 cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca agtaacaaag
    37261 attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat tatgccgaaa
    37321 gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag tgtgatctct
    37381 gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg taaagtaaaa
    37441 gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga tattatttca
    37501 gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa aaaagaaaag
    37561 tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta ctcatggctt
    37621 taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga tataatcaag
    37681 acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg actcaaagaa
    37741 atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac ttcaatgaat
    37801 taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat aaagtaaaaa
    37861 caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa gcagatgagt
    37921 tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa gaaaaagcaa
    37981 gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa actgaagatc
    38041 ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag tctcaattag
    38101 aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc tacgtgactg
    38161 acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa aatgatactg
    38221 atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat acacaggcta
    38281 ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa gcactttgtt
    38341 attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg tgaaagatcc
    38401 tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac ctcgttattg
    38461 gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca cggcatgttc
    38521 gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga gtgtatggtt
    38581 gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg cgtggtaatt
    38641 tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc taccaaaaaa
    38701 gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct ccatatgcca
    38761 atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt atcattcgtt
    38821 caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca catggattgc
    38881 cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc atggcagaat
    38941 acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga gcagatttta
    39001 aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat ccttcttatg
    39061 aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aagctatatt tacaaaggat
    39121 tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct gaaattgaat
    39181 ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac ggcaaagata
    39241 ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca ttgccagcaa
    39301 accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc gatggccgta
    39361 agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt tgggaaaatg
    39421 taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca agtcatccat
    39481 tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac gctggtactg
    39541 gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt agcaaatata
    39601 aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa gcacctggat
    39661 ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta aaagaaaaag
    39721 gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg cggacgaaaa
    39781 agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa ttccgtcaga
    39841 atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa acacgtttgt
    39901 ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca tggggagtac
    39961 cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa acaatcgaac
    40021 atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa gaagcgaaag
    40081 aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt acaaaagaaa
    40141 aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt ttacgtcagc
    40201 gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa tatcgtggct
    40261 ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca tacaaatcag
    40321 ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa tctttaggaa
    40381 acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt cgcttatggg
    40441 tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta agtcaagtgg
    40501 cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat acagaagatt
    40561 tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac aaatatatga
    40621 ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac aaatataact
    40681 tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg tcattctacc
    40741 tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa cgtcgttgca
    40801 tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact cctatcattc
    40861 cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac gttcagttaa
    40921 gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat acctggactg
    40981 ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga aacagcaaat
    41041 tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa gttcgtgaaa
    41101 tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca gactactttg
    41161 aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat gatgttgcta
    41221 ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt cgtaaagatg
    41281 taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc gttgaagact
    41341 tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa gggggtgtga
    41401 catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg ttcacgatac
    41461 cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc gaaagctgct
    41521 ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg gtcccaagct
    41581 tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg agagaagttc
    41641 gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg gctattttca
    41701 accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa tccttgccct
    41761 acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata gatcgttaga
    41821 acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt gaatacatta
    41881 acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa acgtttgcct
    41941 gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa tttgcctgcg
    42001 acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata gcccttgaat
    42061 tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc taacacttct
    42121 tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg gatttctttt
    42181 tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa aatatgattt
    42241 tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc ttctacaccg
    42301 aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca ttgagcctca
    42361 aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt tcctaaataa
    42421 tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg gttcaattca
    42481 aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt gaagccattt
    42541 tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa ttgaggtgcc
    42601 atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa tcggtcagac
    42661 aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg atagtaaaaa
    42721 tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat tgtttctttg
    42781 acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat tacagcaaaa
    42841 tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa tttacgctgg
    42901 gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt agacatgtgg
    42961 atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag ctgacgggga
    43021 ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg tcaacgatct
    43081 catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat tctttttcat
    43141 caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat gtttgtcggc
    43201 gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga caatgttcag
    43261 gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca tgttccaata
    43321 cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact aaaaaaactt
    43381 cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt gcagtcaatt
    43441 gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta gaaatcattt
    43501 cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg ttgactttcg
    43561 ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt ggtgtcatat
    43621 tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa aaacgttaat
    43681 tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac aaaaaacgaa
    43741 agatgataga gaagactcat tctttcgttt ataaaggata tatatataca ttgggaggga
    43801 tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa agagtttgtt
    43861 cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa cgatattcat
    43921 ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg taatcagatg
    43981 taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct tgatactttg
    44041 agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa cttgagagaa
    44101 cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca ctcctttatg
    44161 agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga ataaaagcta
    44221 aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt atgataggat
    44281 acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat agcttttttt
    44341 attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga aataattatt
    44401 gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc tgaagtggct
    44461 attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc tgtaaaaaag
    44521 aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat tgatggtatt
    44581 ttagacggca cgatcctttt accaggatta actggtccat ttacggtata caagcgtgaa
    44641 gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg acaaaaagca
    44701 tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt tcctgatgcg
    44761 cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca agatggttat
    44821 gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac gatccatgaa
    44881 gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg agttcaaatc
    44941 ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc cattccattt
    45001 gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat aatgatcaac
    45061 ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc tttaagcaca
    45121 aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca ggaatttggt
    45181 tctcattatg aaggggatat cgaattcgca acagatttga agactttttt caatattact
    45241 catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca agctaagcaa
    45301 gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac gccaaaagaa
    45361 atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga agcagcaaga
    45421 aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa agacgagata
    45481 atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt attagatgga
    45541 gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga tttaggtgtt
    45601 taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca ttatttccca
    45661 agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta tgagaatttt
    45721 gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga gatttatttt
    45781 gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca atttcatgat
    45841 cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc aatggcagat
    45901 gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg gggattgttg
    45961 tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga tttaagaaaa
    46021 aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac gaatttgtac
    46081 aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc tcaccttctc
    46141 ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta gcggtttctc
    46201 ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac gtggaaccag
    46261 gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt caagtagcag
    46321 atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg gtgggcggag
    46381 ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg attggtacac
    46441 caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa gtgaaaacaa
    46501 tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc attgatcaaa
    46561 ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca gcagatcgtt
    46621 cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac gtgacagcag
    46681 aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata cgaaagaaag
    46741 atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt ttctttaatc
    46801 aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta ccggtaggaa
    46861 gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa ttcaaaaaga
    46921 aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat attacaggtc
    46981 ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg catcgttcgg
    47041 gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa gataataatc
    47101 taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag atttttttat
    47161 acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct gcagtaaata
    47221 aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac aaagaaaaaa
    47281 taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca aggcgcaaat
    47341 aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc agaggagggg
    47401 ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc aacaaggatt
    47461 gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa gccgatattt
    47521 aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag tcgtttactc
    47581 tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta cgcaagtgac
    47641 ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc aggcaaaagc
    47701 tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa ctggtacagg
    47761 gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga tgctttctgc
    47821 agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc aactgctctt
    47881 tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag atagtcccgg
    47941 tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc gtttaccgcc
    48001 ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc cgcttggaga
    48061 aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg aatcttcaga
    48121 aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc cgaatcttgc
    48181 ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac aagaagcgaa
    48241 tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca tgctttatga
    48301 tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg caaaatcttt
    48361 tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag aactttccct
    48421 tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg accgattcat
    48481 ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg tctggtcaca
    48541 agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca cttctctttc
    48601 cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg tggatgctta
    48661 tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc ttttgccaga
    48721 tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt tagaccgtat
    48781 ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat tggatcgtgt
    48841 gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa attttaagag
    48901 ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac aagttgagat
    48961 tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag gagaaagtga
    49021 gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac agactgcttc
    49081 aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag cttttaatat
    49141 gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg tcaaaaacta
    49201 tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat taaacacctt
    49261 cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga tgacaagtac
    49321 catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag aagatattta
    49381 tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag cggaaaagtc
    49441 tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg aaggagtagc
    49501 tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag aactgatcca
    49561 gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat tggaagaata
    49621 tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc catttttttc
    49681 agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag ctgatgatga
    49741 gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg tcttttctat
    49801 tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg atttgtcgat
    49861 tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca tgttagacgg
    49921 attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct caaaaattta
    49981 tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa aatatcaggt
    50041 aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa ataaaataaa
    50101 aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc actataaaaa
    50161 agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat gcttcttgcg
    50221 ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa acaatcatgt
    50281 tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa aaagcagcag
    50341 aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca gataataatt
    50401 tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc atgaaagctc
    50461 aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat atgcaagact
    50521 atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg aaataaatca
    50581 agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag ccatcatggg
    50641 gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg cggctgctaa
    50701 aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat cacttataca
    50761 agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc atatgcaagt
    50821 gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt ttactgattt
    50881 agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac aacttctttt
    50941 ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag gagaactctc
    51001 tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag ttggatggag
    51061 aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt tgctttgcgt
    51121 gatgctttta tcggcacact gcccgcaaca atggcgggat cagtcgctgt aatgatcaat
    51181 gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag tttagcagca
    51241 gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt tgtctggaac
    51301 ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa tcttgcccga
    51361 gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt gattcaaggt
    51421 atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc aaatgtcgca
    51481 gatgcaatca atagttctga gattggtgca acgattgcag atggcacact gtcagttgga
    51541 ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac agttatgatc
    51601 atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat tacgatcaaa
    51661 atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt accagcgacg
    51721 atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc tgatgggcaa
    51781 ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt atctcaaggg
    51841 ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt tggtattcat
    51901 ggaccaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca actaatcaat
    51961 attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt gttagatgca
    52021 atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt tgcatggttt
    52081 ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc taaacgtgca
    52141 gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat caacgaacca
    52201 gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt cttgattgct
    52261 ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt cgctccggta
    52321 tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc aacaggagcg
    52381 gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt gatttgggca
    52441 ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca ataagatgaa
    52501 cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag gaggaactga
    52561 aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt tagaaggata
    52621 caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc tgttctttga
    52681 aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa tcaaagcaca
    52741 gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg tttaaaaaga
    52801 taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt ctttgatcgt
    52861 gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg tcacaggtct
    52921 ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag aagaagcaaa
    52981 ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc gtttttttac
    53041 gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa atcagtaact
    53101 agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa ccgggctcgc
    53161 ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc gacaacgaag
    53221 aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg tgaggttcat
    53281 tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat gcttttagga
    53341 acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat gatcgaagca
    53401 acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag taagctagaa
    53461 ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt tttgtaggaa
    53521 ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt gaggaaatgt
    53581 ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga gaacgttttc
    53641 gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat cggctaacaa
    53701 gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac ctaatggccg
    53761 gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc aatcagatca
    53821 tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat ctggcgccat
    53881 tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa atcagttatc
    53941 aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata aagcgtatgg
    54001 attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg gaaaaccaac
    54061 gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa aaaggcaatt
    54121 accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc gtttatcagc
    54181 ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat gctttttgca
    54241 gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa tattcatgcg
    54301 gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct gtaatgatca
    54361 caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat tatccattta
    54421 aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg aaatggaatg
    54481 aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa gtagaacaaa
    54541 caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta ttagggtatc
    54601 gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa tatgaagaaa
    54661 cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt tgataaaaaa
    54721 cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat ttgaaggaaa
    54781 atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt ttgttttagg
    54841 tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt
    54901 ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg
    54961 aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg
    55021 gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa
    55081 caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc
    55141 gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt
    55201 atcctttgcg gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat
    55261 catttttata taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct
    55321 ctcgaacaat atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt
    55381 tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta
    55441 gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt
    55501 tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag
    55561 ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga
    55621 gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc
    55681 cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat
    55741 cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg gcaccagcaa
    55801 ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta
    55861 aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc
    55921 ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt
    55981 tcaacgacgc tctaatatgg t
//
LOCUS       DAXWLB010000003        49987 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXWLB010000003 DAXWLB010000000
VERSION     DAXWLB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.003"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            140..1360
                     /locus_tag="ACGE4H_000117"
     CDS             140..1360
                     /locus_tag="ACGE4H_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8363569.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1357..4077
                     /locus_tag="ACGE4H_000118"
     CDS             1357..4077
                     /locus_tag="ACGE4H_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8363570.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4061..5005
                     /locus_tag="ACGE4H_000119"
     CDS             4061..5005
                     /locus_tag="ACGE4H_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8363571.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5074..6084)
                     /locus_tag="ACGE4H_000120"
     CDS             complement(5074..6084)
                     /locus_tag="ACGE4H_000120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8363572.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6264..6608)
                     /locus_tag="ACGE4H_000121"
     CDS             complement(6264..6608)
                     /locus_tag="ACGE4H_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8363573.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6601..7035)
                     /locus_tag="ACGE4H_000122"
     CDS             complement(6601..7035)
                     /locus_tag="ACGE4H_000122"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFF8363574.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7173..7910
                     /locus_tag="ACGE4H_000123"
     CDS             7173..7910
                     /locus_tag="ACGE4H_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363575.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7903..9114
                     /locus_tag="ACGE4H_000124"
     CDS             7903..9114
                     /locus_tag="ACGE4H_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363576.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9318..10100
                     /locus_tag="ACGE4H_000125"
     CDS             9318..10100
                     /locus_tag="ACGE4H_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFF8363577.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10170..10832
                     /gene="trmB"
                     /locus_tag="ACGE4H_000126"
     CDS             10170..10832
                     /gene="trmB"
                     /locus_tag="ACGE4H_000126"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFF8363578.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10930..12684)
                     /gene="ade"
                     /locus_tag="ACGE4H_000127"
     CDS             complement(10930..12684)
                     /gene="ade"
                     /locus_tag="ACGE4H_000127"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFF8363579.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12816..13745
                     /gene="comGA"
                     /locus_tag="ACGE4H_000128"
     CDS             12816..13745
                     /gene="comGA"
                     /locus_tag="ACGE4H_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFF8363580.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13702..14751
                     /gene="comGB"
                     /locus_tag="ACGE4H_000129"
     CDS             13702..14751
                     /gene="comGB"
                     /locus_tag="ACGE4H_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFF8363581.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14748..15026
                     /gene="comGC"
                     /locus_tag="ACGE4H_000130"
     CDS             14748..15026
                     /gene="comGC"
                     /locus_tag="ACGE4H_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFF8363582.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15001..15471
                     /gene="comGD"
                     /locus_tag="ACGE4H_000131"
     CDS             15001..15471
                     /gene="comGD"
                     /locus_tag="ACGE4H_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFF8363583.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15443..15790
                     /locus_tag="ACGE4H_000132"
     CDS             15443..15790
                     /locus_tag="ACGE4H_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363584.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15753..16178
                     /gene="comGF"
                     /locus_tag="ACGE4H_000133"
     CDS             15753..16178
                     /gene="comGF"
                     /locus_tag="ACGE4H_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFF8363585.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16150..16509
                     /gene="comGG"
                     /locus_tag="ACGE4H_000134"
     CDS             16150..16509
                     /gene="comGG"
                     /locus_tag="ACGE4H_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFF8363586.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16681..17688
                     /locus_tag="ACGE4H_000135"
     CDS             16681..17688
                     /locus_tag="ACGE4H_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8363587.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17713..18897
                     /locus_tag="ACGE4H_000136"
     CDS             17713..18897
                     /locus_tag="ACGE4H_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFF8363588.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19334..20917)
                     /gene="abc-f"
                     /locus_tag="ACGE4H_000137"
     CDS             complement(19334..20917)
                     /gene="abc-f"
                     /locus_tag="ACGE4H_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFF8363589.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21022..21150)
                     /locus_tag="ACGE4H_000138"
     CDS             complement(21022..21150)
                     /locus_tag="ACGE4H_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363590.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21495..21638)
                     /locus_tag="ACGE4H_000139"
     CDS             complement(21495..21638)
                     /locus_tag="ACGE4H_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFF8363591.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21861..22340)
                     /locus_tag="ACGE4H_000140"
     CDS             complement(21861..22340)
                     /locus_tag="ACGE4H_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8363592.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22413..22697)
                     /locus_tag="ACGE4H_000141"
     CDS             complement(22413..22697)
                     /locus_tag="ACGE4H_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363593.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22775..22969)
                     /gene="ssrS"
                     /locus_tag="ACGE4H_000142"
     ncRNA           complement(22775..22969)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGE4H_000142"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23041..24327)
                     /locus_tag="ACGE4H_000143"
     CDS             complement(23041..24327)
                     /locus_tag="ACGE4H_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFF8363594.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24465..25136
                     /locus_tag="ACGE4H_000144"
     CDS             24465..25136
                     /locus_tag="ACGE4H_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFF8363595.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25102..25590
                     /locus_tag="ACGE4H_000145"
     CDS             25102..25590
                     /locus_tag="ACGE4H_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFF8363596.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25603..26550
                     /gene="prmA"
                     /locus_tag="ACGE4H_000146"
     CDS             25603..26550
                     /gene="prmA"
                     /locus_tag="ACGE4H_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFF8363597.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26551..27315
                     /locus_tag="ACGE4H_000147"
     CDS             26551..27315
                     /locus_tag="ACGE4H_000147"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFF8363598.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27548..29761
                     /locus_tag="ACGE4H_000148"
     CDS             27548..29761
                     /locus_tag="ACGE4H_000148"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFF8363599.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSNLKSIVDKIKAVPDVYSVRRTNG"
     gene            29772..30218
                     /gene="dtd"
                     /locus_tag="ACGE4H_000149"
     CDS             29772..30218
                     /gene="dtd"
                     /locus_tag="ACGE4H_000149"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFF8363600.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30389..31648
                     /locus_tag="ACGE4H_000150"
     CDS             30389..31648
                     /locus_tag="ACGE4H_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFF8363601.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31737..32684)
                     /locus_tag="ACGE4H_000151"
     CDS             complement(31737..32684)
                     /locus_tag="ACGE4H_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8363602.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33078..34370
                     /gene="hisS"
                     /locus_tag="ACGE4H_000152"
     CDS             33078..34370
                     /gene="hisS"
                     /locus_tag="ACGE4H_000152"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFF8363603.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34385..36154
                     /gene="aspS"
                     /locus_tag="ACGE4H_000153"
     CDS             34385..36154
                     /gene="aspS"
                     /locus_tag="ACGE4H_000153"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFF8363604.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36263..37138
                     /locus_tag="ACGE4H_000154"
     CDS             36263..37138
                     /locus_tag="ACGE4H_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8363605.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37404..39287
                     /locus_tag="ACGE4H_000155"
     CDS             37404..39287
                     /locus_tag="ACGE4H_000155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8363606.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39362..39646)
                     /locus_tag="ACGE4H_000156"
     CDS             complement(39362..39646)
                     /locus_tag="ACGE4H_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8363607.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEDAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39636..40370)
                     /locus_tag="ACGE4H_000157"
     CDS             complement(39636..40370)
                     /locus_tag="ACGE4H_000157"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFF8363608.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40499..41122
                     /locus_tag="ACGE4H_000158"
     CDS             40499..41122
                     /locus_tag="ACGE4H_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFF8363609.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41314..42435
                     /locus_tag="ACGE4H_000159"
     CDS             41314..42435
                     /locus_tag="ACGE4H_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFF8363610.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42442..45570
                     /locus_tag="ACGE4H_000160"
     CDS             42442..45570
                     /locus_tag="ACGE4H_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8363611.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45589..48783
                     /locus_tag="ACGE4H_000161"
     CDS             45589..48783
                     /locus_tag="ACGE4H_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFF8363612.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48929..49636)
                     /locus_tag="ACGE4H_000162"
     CDS             complement(48929..49636)
                     /locus_tag="ACGE4H_000162"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8363613.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16293 a         8798 c        10424 g        14472 t
ORIGIN      
        1 tctttttttc gaataaactt attttttgaa gtcggccatt tatcgtaatt tctttgcata
       61 atacgaacaa attttcggtt attgatgaaa aaaaacattg aaaatggtat gataaaagaa
      121 aaaaaggagg aaatttttaa tggtgaaatt tatccatgcg gcagatttac atatggatcg
      181 gtcatttgaa ggactgacaa ctcttgataa aactgtacag gaaaaactat tgaagacgaa
      241 tctgaccgtt ttatcaaata tcattgaaca ggcaataata aataacgttg attttgtttt
      301 acttgcagga gataattttc atcaaaatcg tccttcatta aaaatacaga aacatttttc
      361 agaacagatg aaacggttag aaaagaacca tatacctgta tttataatat tcggaaatca
      421 tgatttctat caaaaagagc ggtattggtt ctcctttcct aaaaatgtcc atttatttac
      481 atccgaaacg gtagagacaa aaaaaataac gataaaaagt ggagaaactg tctctctcag
      541 tggatttagt tatcgtcaac cgtggattca aaaagacaaa gtgatggaat ttccttctag
      601 agaactcaca gattaccata ttgggttgta ccacggggaa cctggcgtct ctcaaaaagg
      661 aaactatgct cctttccaac cctcaaagat gcaagaaaag ggatacgatt attgggcttt
      721 agggcatatc catgtgccaa cggtcttaaa tgaaaagcaa acaattgttt atccaggtgc
      781 gcctcaaggt catactaaaa aggaacaagc ttctacatct attctactag ttgaattaag
      841 taaaggaagt tgccaaatac atccgattaa agtagcagag gtctattgga agacaaagga
      901 aatatcattg aggaaagcaa gaacgacaaa agaggtcatt gttcacattc gtcagcagct
      961 ttcaaaagta gaggatggat ttagtttaat tgagataaaa ttaaaagatt atgaccatct
     1021 caatacagat gttttggaac ggatacaatc aggagagcta ttagactatt tgttagagga
     1081 attctcagat aggataaatg atttctttat ttggcgtatt tctctcatag agaatacttt
     1141 ttatacgaag accccacttg ctgcttctgc taaattgatg gaacagttat ttcaatacta
     1201 tcagacacct caagatttcc aacaaatttt aaatgaagta tatagtcatc aagaagcggc
     1261 tagaatattg agcgaactgc cggaatatca agaagaaaca cttgaaaaag ccaagcagct
     1321 gcttaatcaa gattttttat ttgaggagga tcaggaatga agattttaag gatcgagatt
     1381 gctgcttttg gaaaatggcg tcaaaaaagt tttgactttt attcaggcaa tcagctgatc
     1441 tatgggggaa acgaagcagg aaaatcaacg atttatcaat ttattcaagc aatattattt
     1501 ggtttccctt caaaaggccg taaaaaaaag gattatacac caaaagacgg ctcagcctat
     1561 ggcggcaaaa tatggcttaa acatcctgta tatggtgaat ttgctgtcga gcgctacaag
     1621 cagcaaaatc gcggaaaatc gaaggtgtgg ctgggggatc aagtgggaag tgatgagtta
     1681 ctagaaaaac tgatcgcacc tttgacgaaa gagttattcc agcaggtatt tactttccag
     1741 caagagcagt tgatggaact agatcatttg agagaaaaag agctacatga tgcgctgatt
     1801 tctttaggca tcacgggcag ttctcaagta ttccaaaagc ggcaagaata ttatcagaaa
     1861 gcacaacaat tattcaagaa aaaaggccaa aaattactaa tcaatcaaaa attaaatgaa
     1921 tggaaagagc tgcaagaaaa aattcatcaa aaacaagaac aggaagctta ttttggcaag
     1981 ttgatccaac aagaacagga atacgagtcg caacagcagc agttattggc acaattaaaa
     2041 gaggcgaaca aacaattttc attagccaga cagcaagaac ttaatttttc tttatatgaa
     2101 gaatggcaat ctctcaaaaa aaacagcact acaggtcttc ctgatgagca acttctttta
     2161 gatctggagt ctttctccaa acggtatcag caaacaaatg agcgtttgga agaattagaa
     2221 gcaacactgg aaaagcatag cgggatggat cagcagtcag ccagatatta tttttattta
     2281 gagcaggagc aggaactaaa agatctccag caacttcaat ttacagcgga acaattaaac
     2341 gaagaatggc aaagattaca aattgagact agagaaacaa attcagaatt gaagattttg
     2401 gaacaaaggt ggcattggca gaaaaatccg ttaccggaag aatttttata tgaagaagaa
     2461 tggaagcatc ttctggctga agaaagaagg attgctgatc tagaaagcca gatttctgtg
     2521 cgtctccaaa tagctaaaga acagcaagag atgattgaag cagaagtgaa tgacttggaa
     2581 gagacctatc cagcattatt acaaaatcag tcagagatag caccagtctc ttcatctaaa
     2641 ccgtatatgc tgatggctgg cggagcaata atcattgcag gtatatttgt accaatgccc
     2701 ttgaaagtga tccttttact aattggtgca ggcatgattg gctatttctt ctacaaaagg
     2761 caaccaaaaa ttaaaactaa aaatgatttc tctgagataa aaggaatgtg gcaagaaaaa
     2821 ctaggacaac ttgatctagc cttagctcaa ttagctgaag cagaagaaaa acaaaagaga
     2881 actggagaag aaaaacagat tcttcagcaa gaagtatatc gttttgcgga tcatcggcac
     2941 ttaggaaaga tggatacgct caaactcatg aaagaatatg gacaggaagt catagattat
     3001 cagaaattat atggcgtatt tgagcggaaa cataaaagag aacaggaaat acaagaaaaa
     3061 ctacgacaaa tcgaccaaaa atttgagttt gttaaagaat ggttaccgat acaagataag
     3121 acaatttcag aaaaaatggt attgctttcc cgttttatac aagagatgga acagatgaaa
     3181 tttgccagaa gctatcagca aaatacttta ttgaagcaag aaatcaatca attgaaaaag
     3241 aaacaaaaag tatggataca agaatacagc gaacaattga ctcaagcagg cttatcttat
     3301 ccttcggaag tacctgtctt tttacaaaaa gccaaacaag ccgaacagca gatgaaaaga
     3361 tacaatgaat tatcggaaat gcttactcca ctatttcctg aagagctgac aaaagagaaa
     3421 ttatctgtaa gactgcgcca actgaaagaa aagcaatccc aactccagca agaagaaaaa
     3481 gaagcaagtg aaaatcggca acgactccaa cttcaagtag aagagttgca aagagacggc
     3541 acattggacg aactttatca acaagctagc cggcaaaaag caaaattaca ggaattactg
     3601 gaggaatggg gagcacttga aatcgccggg acattcttag gtgatctttc aacagagatg
     3661 agtgaaagac aattacccca gttgttaaga gcagcaggtg attatttagg tattttaaca
     3721 aaaggaaatt atcagaagat attactgaaa aatgatacac tgactttaac agacggatat
     3781 acagaatttc ccatctatga gttatctaca ggtacgaaag atcagctgat tatggcgatg
     3841 agatttgctt acctctcatt agaagctaac cgttcgatat gcccgatcat cattgatgat
     3901 ggctggcttc attatgatca tcagcgaaag tatcaattag ctgaactttt agctaaattt
     3961 ggagaaaagt atcaaataat ttgtttttct tctgataaag aaatggtaag ctattaccat
     4021 gagatgaacc aaatcgtaaa acaattagaa ggagcaaata atgaaaaaaa ttcgtgattt
     4081 agtaatcgat gagcagtttg aacaatttgt cctgatcaaa agtgcagatg taagaatcgc
     4141 taaaaacgga aaaaaattta ttgcttttac tttccaagat acttcaggta ctatcgatgg
     4201 taaatactgg gatgcttcgg aggaggaaat caataaattt acagccggca cagtcgtttt
     4261 tctaaacggt aaaagagagg tttatcaagg aaaccctcaa gtaaagattc ttcatatgcg
     4321 cttaacaagg gtagatgagc caggagagcc tagtttatac atggaaagag cccctttgaa
     4381 gaaagaagaa atggaagaag aaatcaatca attgttgttt gagatcacca atgcgcattg
     4441 gaatcgtatt gttcgctatc tacttaataa atatcaaaaa caattttttg agtttccggc
     4501 agcaaaaaga aaccatcatg catttgccag tggattagct tatcatactt tgacaatgct
     4561 tcacttaggg aaatcaatcg ccgatgaata tcctgagctg aatcgagcat tgttgtatgc
     4621 tggtatcatt ttgcatgatt taggaaaggt gattgaactt tccggtccta tgtcgacaga
     4681 atatacgctt gcaggaaatt tgatcggtca tctggtattg attgacgaag aaataactaa
     4741 agcctgtctt gcgttgaaaa tcagcgaagc tgacgaagat gttctcgtat tacgtcatat
     4801 ggttttagca caccatggct tattagaata tggctcacct gtacgtccaa gaataatgga
     4861 agcagaaata ttacatcaaa tcgacaatat cgatgcttcg atgcagatga tgttgacttc
     4921 gattcgtcaa acagaaccag gaaaatacac cgatcggatt ttcggtatgg acaaccgaag
     4981 tttctatgta ccaaaagatt tatagcaaaa atttattttt ttcattcaaa acaaaaaaag
     5041 cgagacataa gtcttcgctt tttttgtttt cttttatttt gatgaatcag ttgtttcttc
     5101 agttgaagat tcttttgttg aatcagttga ttttgtgtca gaactctttg ttgaagctgt
     5161 agtttcactt gagtctttcg tacttgatga agttgttgtg aatgcactca acacattttc
     5221 aaaggcatca tctttgattt ttacatttgc atcttttaat tcttcaccga ttactttagt
     5281 tgtgaatgca ttgtcactta gtttagtttc agtagcgata tcttttagtt gatctttgta
     5341 tttatccatg tcattgcctt tattttggtt tttcaccatt ttgacaacgt agtattctgt
     5401 cgcataagaa gtaggatttg ttgtagtaat aacatcggag atctcaccgt ctttcaattt
     5461 gaaagctgct tcttttactt ctgcaggaat agttgttgtc gttgagtcga atttaacttt
     5521 accgccatct tcttttgtct ctgtatcagt tgatttgtct ttggctaatt tagagaaatc
     5581 atctccatca tccgctgatt ttttcacatc ttttgcttca tcttcacttg ataatttgat
     5641 gatttgtgct tctacttctg gatggaaaga tttccaagca gttttcaaat cgtcatctgt
     5701 aatatctacg tgagctttca agccagcttc aaaagctaaa ttgttacgaa tatattcttt
     5761 ataagtatct ttcgtatatc cagcagcttc taattgtgat tcaaaagtat cacctaatga
     5821 tttcgcttgt ttgtcgtatt ctgcatccac ttgcttatcg gttactttat cgccatattt
     5881 gttattgaat actttataaa tgatcatccg ttgtactaag gattggttcg atgattcaag
     5941 ttttgcttca tcatagaaat cagatacagt gatcgttccg cctttcatcg ttgcgatatc
     6001 tttattagta tcgcctgaac aagctgctag agttaaaaca gccaacgcac tagtagctgc
     6061 caaaataatt gatttttttt tcattaaaat ttgcactcct gtaattagtt attattttta
     6121 tatattaact ataccatatc ccatttcctt taaaaacgtt gtcgtggtag tttcaaacaa
     6181 aattcaaaat ttcatcacat tcctaggaaa tacgcaaact tttctcactt atatagttaa
     6241 atctgttgag aaaataatca ttattatatt tgttcaggat actctggcag atcagctttt
     6301 agtttttcta cttgttcatt gatttgtgtg atacgtggtt cggcttggaa tttaaaatct
     6361 tcgatatctt ttttgattgc ttcttggaaa gcaggaatca cagattcagc tgtttctttt
     6421 gtttcgatca atgcgctttt aaaatgattc aggctgttat ttaaatcatt tgtcagagtt
     6481 gttgcttctt ccaattcttc gatcagtttc tttcttgttt cattcccgct cctgggcgcc
     6541 aataacagac ctccgacagt gccagcagca gctccaaata acagtccttt gaaaaagttt
     6601 ttaatcatcg caggacacct gctttacgat tgtttcttga atagctttta gttcttccga
     6661 tccataacta tcttgatgat tgacaaagtg aatagaaaaa tcatcctctt tgctgtagcg
     6721 agggactaaa tggatatgtg aatgaaaaac agattgatag gctagttcac ggttgttatt
     6781 caagatatta agacctttca tttctggaaa agctttttcc aatgcttgag caatcttagg
     6841 cacacgcgaa aaaacctctc ctgcaagttc gggatcatat tcaaaaatat ctgctacatg
     6901 acgtttaggc acaactaatg tgtgtccttt cgttgcttga gagatgtcta aaaaagcata
     6961 gaccttgtcg tcttcatata ttttatagct tgggatttct tgattgatga ttttgcaaaa
     7021 aatacagttt tccatataaa acatcctttc tataaagaat aaatacttca gcttaacttt
     7081 accataagtt aaagctattg taaggaaaaa aacaaaatac cgtcattttc ttcgtggtat
     7141 aataaataaa aataaatggc ggaggaaaaa gaatgagttt aattatcgat catgtcacag
     7201 gggggtacgg acatctccct gtgttaaaag atatcagttt cgatgtacag cctggagaga
     7261 tagtagggtt gattggactg aacggagcag ggaaaagtac gaccatcaag cacattatcg
     7321 gtctgctgga agcttcaaaa ggaacgatag aaatcaatca tctatcctta aagaaaaata
     7381 cggaagaata tcgcaaacaa attgggtata ttccagagac tccttcttta tatgaagaat
     7441 tgacattgag agaacatatc gaagtcactg cgatggccta cgatatacct atggatatag
     7501 caatgaaaag agcagaaaag ttgttggaga cttttcggtt atctaatcgt cttgactggt
     7561 tcccagctaa tttttcaaag ggaatgaaac agaaagtgat gatcttatgc gcctttttga
     7621 tcgagccttc tttatatatc atcgacgaac catttttagg cttggatcca ctagctatca
     7681 atgcattgct tgaattgatg gtagaaatga aaaaagaagg agccggtatt ttgatgtcta
     7741 cgcatatttt agctactgca gaaaaatatt gtgatcgttt tgtggtctta cataacggag
     7801 agatacgagc agacggttct cttgagatgt tgagaaaaga atttcagctg ccggattctt
     7861 ctttggacga aatctatatt gctttaacaa aggagcaggc acatgagtag tttttttcaa
     7921 aaacgtttag caagacatca aaagaaaatg atgaaatata tgcgttatgt attgaacgat
     7981 cattttgttt tagtttgtct tttcttgatt ggcggtgtgg gattatatta ttctaattgg
     8041 ttgaaaaccc tgcagccacc gtttgcaatt ggagggctca taatctctgt attttggatg
     8101 ctctgtttat tcgtgggtaa gatggctact tttgcagagg cagcagatat tgttttttta
     8161 cttcctaaag aaaaagaaat gcaaacctat ttggaacgag cttttcgcta ttcttgcatt
     8221 tttccgttga tctttttgat attggcatgc ggattggcca tgccattagt ggtggttagt
     8281 acggagcaat ccttttcgat gtttttcgtc tatctgatca tattatggag tttgaaattc
     8341 agtcatctgc aaatcaagag agcagaattg tttcgactag atcaacaagt tattcgttat
     8401 tggaaattag gctggttttt ttcttctctg atctttttgt ttctatcatt ttattggcta
     8461 atatgggcag gaatagcagg tgcattagta caggcaagtc tatattactg gtttttgtgg
     8521 aagcaacaag aaaaaggatt ggattgggag aaaatggtgc atgtagaaga acataggttg
     8581 catcagatct atcaatttat caatttattc acagatgttc ctgaaattac ggcaaagatc
     8641 aaaagaagac gctatttgga tcctatattg actcatatta aaagagaatc aaaaaacact
     8701 tatctttact tgtttgcaag aagattggca agaggttctg attttagtgg tctttatttc
     8761 cgattagtaa ttataggtgg gttattgatt gccggagtag tcgactttta ttttagtctg
     8821 attatcggtt tcttattttt atatttgatc ggtttccaac tgttaccttt agcgaaccag
     8881 ttccgctata tggttctcgt tcagctttat ccaatccata agaatcagaa gaagcaggcg
     8941 attcagtatc tactcacatg ggtattaatt atcgcgtcgg ttatttttgg tttgattgct
     9001 gcagtcgtat tagatgggca gcagcgatgg gttccgctag cggcttattt gattgttact
     9061 atgctctttg ttaaactata tgtaccaact cgtttgaaga agatgtcaga ctgaaaacaa
     9121 agctgtgtag ggtaaaaatg agaatgaaaa atagagcgtt gacagtaaga atgctgttcg
     9181 ttctattttt tttgagaaaa gctggttttt tgaatggcaa ttaaatgaaa aagaacgtat
     9241 atgtgcttga cgtgtagtgt tcaggcgact agaataataa gtactgcaaa acaaagagta
     9301 agaggacggg agaatgcatg atggagtttc agttagacca agagtggcgg ttacgtccca
     9361 tcaaaggcga cacaggaaaa acatacatag gcatgcgtga tgaggacaaa gtctttatta
     9421 aacgaaacac gactccgatg ttggctgctc tttcaaggga aggaattaca ccaaaacttg
     9481 tttggacaaa acgaacgggg aatggcgata ctttgacagc acaagagtgg ctggaaggtc
     9541 gcttgctgac tcctaatgag attggtcaaa gaaacgacgt gattgatgtt ttgtaccatt
     9601 tacatcattc aaattctctt aaaaccatgc tgacccgtat cggtggtcgt gtcatgacgc
     9661 cggagaagat gctgcaaaaa tatgaaaaag aattaccgga agaactccag aaaaatcaat
     9721 ttctgcaaat tgtccatcgt tttcttcgtg agcagatccc gtcaatagat aaggaaaact
     9781 tatcagtagt acacggagat gtaaaccatc gtaattggtt agtatgtaaa aattatttat
     9841 atctagtcga ttgggattca atcatgtttg cagaccctgc ggtggatatt gcaacgattt
     9901 taggaaatta tgtgccactg tctagctgga atcagtggct gatcagttat ggcatacgcc
     9961 cgactgacga aatgcttgaa aaatttcatt ggtatgcggc catgaatttg ctccaagaaa
    10021 tcaccagata ttgcaaacgt gaagatcatc gacggatgaa cgaagaaatt ttacaattaa
    10081 aacgaatttt cagtggctga ggtttggccc aaactctgcg gagatttggc cagacctttt
    10141 taattagagg aaaggaatgg agataaataa tgcgtgtccg aaatcgtccg ggagcagcgg
    10201 aaatgttagc ggctcacccg aattttgtaa ttagtgatcc aacattgtgg aagggaaaat
    10261 ggaatgaatt gtttgaaaac gatcatccaa tccacattga gattggtatg gggaaaggcc
    10321 agttcatcac tggaatggca aaagcccatc ctgaaatcaa ttatattgga gtagaaatgc
    10381 aggtcagtgt tgtttcgatt gcactagaca agttgatcga acaaccgctt cctaacctga
    10441 aacttctaca tgtggatgga tcagcgttga cagagtattt cgctgacagc gaagtagatc
    10501 aaatctatct gaatttttcc gatccatggc caaaaaaacg tcatgaaaaa cggcgattga
    10561 catataagac ttttcttgct gtagatgaac agattcttcg tccaaatgga gaaatccatt
    10621 tcaaaacaga taatcaagga ttgtttgagt attctctggc aagtttttct caatatggca
    10681 tgatcctcaa acaggtatgg ttagatttac atcaaagtca atttgaaggc aatattatga
    10741 cggagtatga agaaaaattt tcttcaaaag gtcaacgtat ttatcgagta gaagctcaat
    10801 ttcaagataa gaccaaaaag aaaagtctgt gaaactaact ttctgctttc ataaaaacat
    10861 taatatccat aaataaatcc cagaaaatga acattacaga tgtaatgtat tttctgggat
    10921 ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa tcaattggaa
    10981 gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga cagataatgt
    11041 taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt gatgtgcttt
    11101 agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc
    11161 gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc
    11221 acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg tcccaacgac
    11281 aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa
    11341 tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat
    11401 ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc
    11461 taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg cttgtgaaga
    11521 agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt ttggcagaca
    11581 acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc cagattgaac
    11641 aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat cgtctgttaa
    11701 gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat caaaacattc
    11761 tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc
    11821 ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca gtttatcatc
    11881 ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta gcgatgccag
    11941 atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca aacgttcttt
    12001 agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag gataaacatt
    12061 taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat gctggtgcat
    12121 caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat agttcatgac
    12181 ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt ttgcatttaa
    12241 atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca taaaaaagat
    12301 attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac tcccagcaat
    12361 gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca aggatacttt
    12421 tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat cgatgaatcc
    12481 tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt caccgattcc
    12541 tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg taaagacgtt
    12601 gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg ctacttcgat
    12661 atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt ataagtataa
    12721 cataaattcg gtttttgcca atattttttt gtaaattcat tttatttgaa acaaaaagat
    12781 caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga ttatcggatc
    12841 aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg ccagcttcag
    12901 aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag aaagtctctt
    12961 cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg gatatcggag
    13021 aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag gaagttcgtc
    13081 tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt cggctattat
    13141 acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca actattttac
    13201 agaaaaccgt cagacgtgga ttataccttt tcagcggacc agtcggatca ggcaagactt
    13261 ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc gaagatcctg
    13321 tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata gcacagactt
    13381 atgatacact tttgagactc gcattacggc atcgtccaga cttgctaatc attggcgaga
    13441 taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg caccgggttt
    13501 ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat gaactgacaa
    13561 agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa cttttggaag
    13621 atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa gaatcagtaa
    13681 aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag tcagaactat
    13741 tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg agaactgctt
    13801 ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg ttcgtttcca
    13861 cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta
    13921 ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc tgaacgtcat
    13981 gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt tgaaggattt
    14041 cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt tttgctaggt
    14101 atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg aatggtcatg
    14161 caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct actattcatc
    14221 ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt gtctcctgtt
    14281 gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc cttgtatcaa
    14341 tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga attaaggcaa
    14401 atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct tgcctctaag
    14461 ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa ttatcgtttt
    14521 ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag tttaggtaaa
    14581 gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct tcatcactgg
    14641 atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt attagtttat
    14701 gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg aaaaagagaa
    14761 aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta ctgatcatca
    14821 gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat gtagaccaag
    14881 aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat gcgcttcaaa
    14941 ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc aaacgagaac
    15001 atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata tacgttgttt
    15061 gaatcactct tactgatttt attacttaca acgatccttt cttttccagt tctcgcattt
    15121 tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga aaaacgaatc
    15181 tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta ttggaatagt
    15241 gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg ggaagtatta
    15301 gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc tgctagaacc
    15361 gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga gcggacgatt
    15421 acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata aaacaggatt
    15481 tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct acttagtgag
    15541 ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac tgctatttat
    15601 tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac cagagcgaag
    15661 ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa tcgaaaaagt
    15721 gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt ccagcattta
    15781 cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc acattcaatc
    15841 ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat caaaaagagt
    15901 ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat gtctctgtga
    15961 aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa agatatcagg
    16021 caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca agtatcactg
    16081 agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt gaaaatggag
    16141 aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa gggctctatt
    16201 ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc agcattagaa
    16261 agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat agcaaaaata
    16321 atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga aggagttttt
    16381 tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt cttagaggtg
    16441 gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac agatactagt
    16501 acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact gtttttaata
    16561 aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt
    16621 gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat gaggtgcacc
    16681 atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat tcagctttta
    16741 cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga aaatttaata
    16801 gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac gcaacggatc
    16861 acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg gcgcagactg
    16921 tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa ccatcaactc
    16981 actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata cacaaataaa
    17041 aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt attaacagtt
    17101 cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga tattgatgat
    17161 acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt tcaatacttt
    17221 catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat cagcgatttg
    17281 ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa
    17341 ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt gaagccggat
    17401 ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag tgaagaaatc
    17461 aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc agatgaattg
    17521 ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg accacaagca
    17581 caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg
    17641 acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc attcaacgag
    17701 aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag tttgaaatgg
    17761 caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt
    17821 ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga acaaatcatg
    17881 gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat tgaactgcaa
    17941 attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca cggaggagaa
    18001 acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc tgaattagca
    18061 gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt
    18121 ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac aatgccaaag
    18181 caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt aagaaaatat
    18241 ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa
    18301 ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc gatcacagca
    18361 gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc tggggtgaca
    18421 atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat ggaaaaatta
    18481 gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct gctaggccta
    18541 acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga acatattcgt
    18601 ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg
    18661 atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga cggacatgta
    18721 cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat
    18781 gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag agtattagtg
    18841 atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc
    18901 aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg catgtgtttt
    18961 ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt ttttacagga
    19021 tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg cattttattt
    19081 attgattttc aggaaaatcc caatatttta ttttcattca tctttataag ggacattaca
    19141 tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt tattcttcgc
    19201 tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata aaaacttgat
    19261 tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt
    19321 gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc tgctcctgat
    19381 caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta
    19441 ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca tagctgtcga
    19501 ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag agtttcagca
    19561 acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc acatcgcttt
    19621 gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga
    19681 tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc ggcaaataac
    19741 cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc tcaccgagta
    19801 ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct aaatgatctc
    19861 cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac tgctggtgca
    19921 agttttgaat gatgaaaact tctttgccag attttccacc attagacaag ctttgcagcc
    19981 gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt
    20041 ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga cgaaaacgac
    20101 gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt tttgtcagct
    20161 cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga tactcaatca
    20221 atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat tgccgatcat
    20281 gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac cagcgaatac
    20341 tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca tcggctgcta
    20401 aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt
    20461 cctgacggtg tggaatccct aagccttgca agatcgctgc aatccggtct tctaatacgt
    20521 aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga cacttctcta
    20581 agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt ttgaggtcct
    20641 gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa actgtaagtt
    20701 cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg ctgtcgacac
    20761 cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg ataccgacta
    20821 aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat ggattcccgc
    20881 cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt gagaccactc
    20941 aaaaaggcca tggttatcca tggcctgtgt tatttaaact cagattatca tgagaaaaat
    21001 accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc
    21061 aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt ggtaattttc
    21121 attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg tagttgtctt
    21181 ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac ttaatattta
    21241 tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg tctcgtggac
    21301 cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt gtttgataaa
    21361 aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat taaacagctc
    21421 ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa
    21481 gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg
    21541 tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc catcgatact
    21601 tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca ataagctttg
    21661 gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg ttcacttttt
    21721 tttattttac ttgaactcat tgactgtttt ccctttattt taaacattat aaagaccgat
    21781 gagtgcttgc cgcgtctttt gtttttatca cggttttttg tgcaaaaaaa cctgccgata
    21841 gttggcaggt cgtctacgtg ttaagctttg gtgtggtctt tagctggcaa ttggttttct
    21901 aaatcagttc ggacaacaag cacatcgcaa gctgcgtttc taataacata ttcagaaaca
    21961 gaaccaataa ataaacgttc tacagcattc aatcctgtgg ctcctaacat aatcaaatct
    22021 acttgattat cttctgggat ttctctggca ataatttgtt ttggtgagcc atattctacg
    22081 acagaggtta cattattcaa gccagctttc ttcgcattgc tttcataatc tgccaatgtt
    22141 tgctttgcca tttcagtagc ttgttctgca agcattccat caaaggatga aacggtttgg
    22201 aaagaacgtg tgtcaatgac atgtgctaat agaagttctc cgttatttcg gatagctaca
    22261 ttgaccgcct ttttaaaagc taattcagct tcatcagaac catctacggc aaccatgatt
    22321 tttttgtatt gttgtaacat aagcattcac tcctcaagtc aatcgtattg tactctttta
    22381 ttgtagaaca ctttagggaa aaagagaatt atttactgct ttggttcagc aatttttttt
    22441 gcaaatcgga tactgaaggt ggcagaaaca ataattacga tcagtaaaaa gaccaaggca
    22501 ataaagcgtt gattgaaaaa agctgttaaa atacccatta cgcctaaaac agcatatgca
    22561 gctccgtaaa attgtaggaa tccttgattt ttatcatttt tgttgatcaa tacaaaaaag
    22621 atatcctgtt tattaaataa ataatagcta acgaataata atactaaagt ggcgataatc
    22681 attaaaatcc taatcatgaa caaatcctcc aaaataagca acaaatagaa aaagcaattt
    22741 gccaagagca aattgcttta ttctttaaaa gatggtaatc tccgttaatg ccgttgtttg
    22801 tcctgtagta ttgatcccgc gaattcaagc aggtgggtcc catggtcgta gcttcgaact
    22861 accaaatgat aaggcatgtt accagcagtg acacaagacg ggtcccaaga tatcaataaa
    22921 aatgttcggt caacacacaa agaggacaat tcgaacatca cagatttccc acctctatag
    22981 tagcatatga atttaaaaag agcaatgaga gagctcgcag aaacagaacc gttttcttgt
    23041 ctagtttttt ggaggatgct ttttccactc ttttatacgg taatactgtt gacccagagc
    23101 ctgttcatat ttccctgtat cttttggctg ataatactga gcattcttca gtttatctgg
    23161 taaatattgc tgattgaccc aagcctgatc aaaatgatga ggatactgat aacccacgcc
    23221 acggttgagt gatttcgcac ctttgtaatg gctatctctc aaatgatcag gaacatctcc
    23281 agctttgccc tctcgaatat cagcaagtgc tgcatctaat gccatgtagg cagaattgga
    23341 ttttggcgac aagcaaagat cgacgacgac gtctgctagt gggatacgtg cctcaggcaa
    23401 tcccaatttt tcagcagcta atacagcgtt cactgtacga gccgcagcag caggattacc
    23461 taaacctata tcttcataac cgataaccat cagacgccgg caaatacttg ccagatcacc
    23521 tgcctcaact aaacgggcga gataatgaag tgcagcatcc acatcacttc cacgaatcga
    23581 tttttgaaaa gccgaaataa cgtcataatg agcatctcca tttttgtcat gggtaagagc
    23641 ttttctttgg acacattctt caatgatcga cagtgtaaga tgaatccttc cttctttatc
    23701 tgaaggagtc gatagtgtag ccagttccag cccattcaat gcactccgca aatcgccgtt
    23761 cgttgcacgc gaaaggtgaa gaagagcatc ttcgtctaac tggattgctt gttgtccaag
    23821 gccgcgttct ttatccctta aagcatgttc gactgccaac tggatgtctt gttctgttaa
    23881 tggtttgact tcaaagatct gtgtacggct gcggatcgct ggattgatgg taatataagg
    23941 attctcagta gtcgctccga tcaagatgat tcggccactt tctaaatgag gaagcagaaa
    24001 atcctgtttt gttttatcta atcggtgtac ttcatccaat aagaggatca ctgtaccact
    24061 catttttgct tcttccgcta caacttgtag atcctttttt gaatctgtag cggcgtttag
    24121 catgcgaaat gcataattag tagagccggc gatcgcactt gcgatactcg tttttccagt
    24181 accaggaggt ccatatagaa tcatggaaga gagcatgcga gcatcgacca tgcggcgtat
    24241 gatttttcct tccccgacta gatgctgctg accaacgact tcatcgatcg ttcgaggtct
    24301 catgcgataa gccaatggct gttgcatagg attttcctcc ttcttgtttt tacgtccctt
    24361 aattatatca tatttagcaa gatgcctaga aagaacagcc ttctgttgtt ttgttttcat
    24421 agcttatcca actaatattg tgtatgataa agtaggtgag aaaaatgacg gataaacaaa
    24481 agatagatga atttttttgg aataaaacga cgccagaagt ggcacgggat ttattgggga
    24541 tgtatttgga gcatgacaca ccagaaggaa gattagctgg atatattgta gacgcggaag
    24601 catacctagg tccagaagac gaggccgccc atagctatgg tttaagaaga acaccgcgtg
    24661 tgcgggcaat gtatgaaaaa ccaggtacga tctaccttta cacgatgcat actcatcgga
    24721 ttttgaacat cattactcag ccagaaggga ttcctcaagg agtaatgata cgtgcaatcg
    24781 aacccgccgc aatgattgat caaatgagta aaaatcgtgg cggaaaaact ggtccagata
    24841 tcagcaacgg tccgggaaaa ttagtggaag cattagcaat tcctcaagaa ctttatggtc
    24901 agtctatagc agatagttcg ttacgcttag tttttgagaa aaaaaagaca ccaaaaaaga
    24961 tcaatgcttt gcctagaatc ggtatcccaa acaaaggtgt atggacagaa aagccgttgc
    25021 gttttgttgt gtcgggaaat ccttatatct ctttacaaag aaaaaaccaa atcgagaaag
    25081 attggggttg gaggaaagaa aatgaaaaag aaggatctat taacatattt agatgaaata
    25141 attgaaaaac aagtgacaga ctatgatgta gctttagatt gggatacaaa aaatcatacg
    25201 atcgagatcg ttgttcgctt gtttgcagaa aataaagcac aatttgagat tgatgatgca
    25261 gaagggatcg tttcgcaaga accaattatc gaatttgaag acggcgtact tctttttaat
    25321 cctcagaaat ctgtttttga tgaacgcaat tatctagcag taatccctta tgaaggaaaa
    25381 aaagggttgg caaaatcagt agctgattct ttagtggaat atctaaatga agtattagct
    25441 caagggcaaa gcgatttgct tgattttttg gatgaagacg atgacgaagc tgtattcgag
    25501 cttcactggg ataatcagca actagcagaa ttagtagaag taaaacagca aaatgaagca
    25561 gatacttatt taccgtaccc aagttattaa ggagtgacag atatgaaatg gaatgaagta
    25621 aaagtagaaa ctgaaagtga agcagtcgaa gcagttgcta atattttgat ggaggcaggg
    25681 gcgagtggtg tcgcgattga agattcttta gatgttgaac attttcagtc ggatcaatat
    25741 ggtgagattt tagataaaaa tgattttaca tcgctgaaag aaggcgcact agtcatggct
    25801 tattttccgg aaacgacttt cttaccggaa atcttgcctt ttatcaaaga tcgtatcaca
    25861 caattacctg agtttggtct agctattgga aaaaataaag taacagttag tgaagtagag
    25921 gaaagcaact gggcaacagc ctggaagaaa tactatcatc cggtgcgtat tacgcgaatg
    25981 ctgacggttg cgccaagctg ggaaaactac cagacagaag atcctctaga aaaaattatc
    26041 acactagatc ctggtatggc atttggcaca ggcactcatc ccacgacttc cttgacattg
    26101 acagcactag aaacagtctt gcgtggtggt gaaacattgc tagatgtggg aactgggtcg
    26161 ggcgtgctaa gtattgcagc aaaatatctt ggtgcaaaag atgtctatgc ctttgattta
    26221 gacgaagtag ccgttcgttc ggcgaaagaa aatatggata tgaatgaaat agccaaagat
    26281 gtccatgtat cagctaacga tcttttgaca ggaatcgaga ttgaaagtga tgtaatcgtt
    26341 gcgaacattt tagcagatat catcttattg atgatcccag atgcttggcg tttactgaag
    26401 caaacaggca cgctgatcgt ttcaggaatc attgaagaga agaagcaaat ggttctggat
    26461 gccatgttag aacaagggtt tatcgtggat caaattttcc agcaaaaaga ctggtatgca
    26521 attattttga aaaaaccaga ggtggaatag atgcagcggt attttttaaa agaatcatat
    26581 gaatcaaaag actattttgt tttagaaggg gaaaactatc accacattgt acgggttatg
    26641 aggatggaac ccgaggatga agtttttttg gcattcaatg atcaaatagc cattgttgca
    26701 gaaatcacgg agattactga agaggctgtt tatctgaaag aaattaaaaa agaagaaaat
    26761 caaaaggaac tgccaagttc gattaccata gcgagtggct atcctaaagg ggaaaagctg
    26821 gattggatcg ttcagaaatc cactgaatta ggagcgcaag cttttcttgg tttcccggca
    26881 tcagcctctg tcgttaaatg ggatgcaaaa aaacgtatga aacgacaaca acggcttgaa
    26941 aagattgctc aagaggcagc ggaacaatcc catcggcaga tcgtgccatc agtagagtta
    27001 ttggaaacga aacaagattt atttgagcgg ctagatgatt atgacgtggt cttggttgct
    27061 tatgaggaat cagcaaagca aggcgaaaaa tcaaatgcag ccaaaattct tagtgaaaca
    27121 aaaccagata gtcgaatctt agctgtcttt ggaccagaag gtggattatc ccgcgatgaa
    27181 gtcgattatt ttgtagaaca caaagggata ctttgtggat taggaccaag aatccttcgt
    27241 acagaaacag cacctcttta tttattaagt gctgctagct atcaatggga attaatgaat
    27301 ccttctttca gctaacgttc ttcattaata aaagagtatc gacagaaaga attgaaaaaa
    27361 tagtcaacaa tttgtataat agaaacaatt atttatacaa tggatacgaa aaatcgtttg
    27421 aaaaagcttt taaaaagcga acatttccgt aaaaaaacgt ataactgcac ggattttggg
    27481 acaattatca aacagatatt tgtcccgatt cccgttttac cgaaaaaaga aagggggatc
    27541 gattcctgtg acaaaagaag aaattttaac gggacccggt gtcataaagc tcgttagtca
    27601 atacatgggt cccgaacatg tagcatttgt tgagaaagca tgcgaatacg cgactgctgc
    27661 acatgacgga cagtttagaa aatcaggcga accctacatt attcatccta tccaagtcgc
    27721 aggtatatta gcagatttaa aaatggatcc ccatacagtg gctacaggct tcttacatga
    27781 tgttgttgaa gatacagaaa tcactttaga agatctgaga gaagaatttg gcgatgacgt
    27841 tgctatgtta gtggacggcg taaccaaatt agggaaaata aaatataaat cccacgaaga
    27901 gcagctagca gaaaaccacc gaaagatgct gctagcaatg gctcaagatt tacgagttat
    27961 catggtcaaa ttagctgata gactacataa catgcgtacg ttgaagcacc tgcgagaaga
    28021 taagcaaaga aggatcgctc aggaaacttt agaaatctat gcgcctcttg cacatcgtct
    28081 agggatcagc cggatcaaat gggaattaga agatacagca cttcgttatc taaatccaaa
    28141 acagtattac cgtatcgtcc acttgatgca gacgaagaga gaagaacgtg aaaaatacgt
    28201 aagtggtact gttgaagata ttcgaatagc gacagaagag ctggggattt ttgcagaaat
    28261 ctatggacgg ccaaaacaca tttattcaat ctatcgtaaa atgaaagatc agaaaaagca
    28321 gttcaacgaa atttatgact tgctagctat tcgagtgatc gtagattcaa tcaaggattg
    28381 ttatgctgta ttaggagcaa tccacacaaa atggaaacca atgcccggca gatttaagga
    28441 ttatattgcc atgccaaaag ctaatatgta ccaatctttg catactaccg tgatcggtcc
    28501 ggcaggaaat ccggtagaaa ttcaaatccg aacacaggaa atgcatgaaa tcgctgaatt
    28561 cggggttgct gcacactggg cctataaaga aggaaaaaat gaaaaagtag aaccagatgg
    28621 tatgacgaaa caattaagct ggttccatga gatactcgaa cttcaagacg aaagctatga
    28681 tgcttctgaa tttatggaag gcgtaaaagg agatatcttt agtgataaag tctacgtctt
    28741 cacaccaaaa ggagacgtta ctgagttacc aaaaggatcc ggaccattgg actttgcata
    28801 cagtatccat acagatatcg gtaacaaaac cactggtgca aaagtaaatg gcaaaatggt
    28861 gcagcttgat tacaaattga aaaacggaga tatcattgag atcatgactt ctccaaattc
    28921 atttggccca agtcgcgact ggttgaaatt agttgctact agcaaagcaa gaaataagat
    28981 caaacgtttc ttcaaagccc aagatcgaga agaaaatgtg atcaaaggcc acgaatccgt
    29041 ggtcaaatgt attacagatc taggatttac gcctaaagat attttgacga agaacaaact
    29101 gcaagaagca ctcgatcgtt ttaattatca aacagaagat gatctctatg cagctgtagg
    29161 gtatggagaa gttagcccct tgacgatggc caatcgtctg actgaaaaag aacgtaaaga
    29221 acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc atgaatcagc cgaaaaaaga
    29281 acctgacaaa atgaaagtac gtcatgaagg tggcgttgtc attcaagggg tagaaaactt
    29341 actaattcgt atcagtcgct gctgtaatcc gattcctggt gatgatatcg ttggttatat
    29401 cactaaaggc agagggatat ccattcatcg tcgagattgt ccgaatgttc agcctgacaa
    29461 accaaatgta gcagaacgtt tgattgaagt cgaatgggaa gatacatcga atacacgaaa
    29521 agagtatgat gcagatttgg aaatttacgg ctataatcgt tcaggcttat tgaatgatgt
    29581 acttcaaaca gtcaatgcgc taacgaaaaa tctcaacagc gttgaagcac ggacgaataa
    29641 agataaaatg gcgacgatcc atttgacggt tggtatccag aatttatcca acctaaagag
    29701 tatcgtggat aaaatcaaag cagtacctga tgtctacagt gtacgccgga cgaatggata
    29761 ggaggacaga tatgagagca gttgttcaac gggtaagtaa agctgctgtg tcagtcgatc
    29821 agcaagaaat tggaaaaatt ggctacggcc tacttgtttt attaggcatt catgaaaccg
    29881 atacgcaaaa agatgtggac tatctggtaa aaaaaattgc acaaatgcgt atttttgaag
    29941 atgaacaagg aaaaatgaat attagtgtag aagatgtggg aggacaaatt ttaagtatct
    30001 ctcaattcac actgtttgcg gatacgaaaa aaggaaatcg accaagcttt gtggaagccg
    30061 cacgaccaga aacagctatt cctttatacg aatcattcaa tgaaggaatc agaagtcgcg
    30121 gcatcacagt cgctaccgga caattcggag cagatatgtc ggtttctctc gtcaatgatg
    30181 gacctgtaac gatcattatt gattctaaaa acaaataaag taatctggtt ttcactagta
    30241 aaaactatga aacacgtcgc ttaagttaga ctgataaact ctaacttaag tggctttttt
    30301 gtctgcaaaa agcgcttctt ttgtcagcag tcggaaaaaa agttaagctt aaattataca
    30361 agttattttt tataggaggt tgctgaagat gaaagaagta ccagtagtag atatcaaaat
    30421 cacagattta aaaaaattgt atcaaaaagg atacaacatg ttggaggagt tacgccatga
    30481 agcggacgct cccgtcgtca aagcaaagat tttcaataaa gaagctatca cgatctacgg
    30541 atcatcagca gcaaaagtat tttatgatcc aaggaatttc aaacgaaaag gtgctatgcc
    30601 aaaattagta ttgaagacac tttttggtca aggtggagta cagactttgg atggagctgc
    30661 tcatcaccat cggaaaaata tttttatgga tctaatgaca ccggaaagaa tggaagatta
    30721 tcatcgtatc ttagataaga atctgacaca agcacttgaa gcacagcatg gtcaatttga
    30781 actatttgat ttaagtaaga tggttttctt tacatctatt tgcgagtggg caggtatcaa
    30841 tttatcagcg atttctaaag atgaggtcga aaaattagca gaatatcaaa tctctatgat
    30901 cagcggaacg tttacttctc cgattgatca cataaaaggt gtcgaaaatc gcaaaaaatc
    30961 agaaaaatgg gcgcaaggct tgattgaaga agctcgacaa aatcctgtgg ctggaaaaga
    31021 aaatgtggca ttatatgctt ttgccaatgc cacagattta gatggtcagc ttttacctct
    31081 ggaagtggca gctgttgaat tattgaacat catccgtcca acggttgctc taactgtgtg
    31141 ggcggcattg atgggacatg cacttttcag tagaccagat ttgtatcagc aattaaaaaa
    31201 tgacttttcg acgttacaag atccgtttat tcaagaaatg agacgctatt atcctttctt
    31261 cccaatgcta cctgcgattt cgctgaaaga agtagaagta gatggttatc gtataccaga
    31321 aggcagttgg gttattttag atttatatgg aacagaccat gatgaacgaa cagttgaagc
    31381 gccagattcc tttatgatca aacggtatgt agggaaagca aaagatattt cctataaaga
    31441 agaatatgag atgattgcac aaggcggggg aaattttcga caaatgcatc gctgtgcagg
    31501 agaatggatc actcttcata gtctacgtgt gttttccgat cagctagtaa ataagtttga
    31561 gttctctgta cctgaacaag attggacgat tccattcaat cagtttccga cttatccgaa
    31621 tagccgagcg ctactttaca agaattagat tagcataaac ttatcgaaaa aaaggaagat
    31681 caaacagtgc tgatcttcct tttttcatat tactataaat tgataaggtc aattgatcaa
    31741 aatccacgaa atgcttcatc aatggtttga taatcttcat tggataaatg caaatctaaa
    31801 gcttggacat tgtcagatac ttgctctgga cgcttagctc caggtataac tacagacact
    31861 cgtggattct tcatgtacca agctaaaaca agttgcgcag gagtggcttg gtatctttta
    31921 gcaatggggc gaagtacatc gacagcggtt aaaatttctc tgaaacgttc tccttggaaa
    31981 tctggatttc tttttcgagg atctccttca ccaaattgtt tttcctcacc aagttcatat
    32041 tttcctgtca gcaaaccgga agcaagagga aaatagggaa caaaactgat tttgtttttt
    32101 tcaagataag ggaataattc tttttcagcc tgccgatgga tcaaactgta tttatcttct
    32161 actacatcga catatccatc tgcattcgct tctttcaatt gttctaacgt gaagtttgac
    32221 acaccgactg ctcggatttt tcctgcttct tttaattcgt gcaatgtagc aactgattca
    32281 ttttttggtg tgctttcatc cggaaagtgg atataaaaaa tgtcgatgta atcagtttgc
    32341 agacgtttta gtgcatcttc caccgattgt ttgagaaact caggagaatt atcaaatgtt
    32401 cgccctttat ttggtacatg agcagctttt gtagcgatga cgacacgtga gcgatcatat
    32461 tctttttcct tcagtacctc gccaatcagt tcttcggaac ggccgtttcc gtaagcataa
    32521 gcggtatcga tcaaattgat cccgctattc aaagctgtac gaactacttg ttttcctgtc
    32581 tcatcttcca aaccgtcaaa aagattatgt cctccaacgg catttgctcc taatccaagc
    32641 tgttctgcgt agacttgtga atgtccgatt cttatttctg ccatagctga ttcctccttt
    32701 ttgtctagtc taaaacaaaa taccaaaaag ctgaactaaa acgtttttgt gaggggaaat
    32761 agtcagatat ctcttgactt tgagactggt ttttagtagg ttataagtaa tatcgaatgt
    32821 caatgaaaga gcgagtaagt ttttgaaact ttacaagaga ggatcatcga ggctgaaaat
    32881 gatctaaagt ttcattagcc taaagacact ctggagatgg acgagtgaaa agccgtcccg
    32941 cgtacgagcg ttattcgtaa gaggaaagag aaatctttca aaattaggtg gtaccgtgta
    33001 tgaatttcat tcacccttgt tcatgatgaa caagggtgat ttttttattt acatagctaa
    33061 gaaagggaga gaaaactatg agtttccaaa gaccaaaagg aacaaatgat attttgccag
    33121 gcgaatcaga aaaatggcag tttgtcgaag aaacagcacg tttattattc agagattatc
    33181 agtataatga aatgcgtaca ccgatttttg aacactatga agtgatatcc cgaagtgtag
    33241 gggatacaac ggatatcgtt tcaaaagaga tgtatgattt ttatgacaaa ggggaacgcc
    33301 atgtgacgct tcgaccagaa ggtacagcac caatcgttcg ttcttttgtt gagcataaat
    33361 tgttcggccc agagtatgcg aaaccgttca aaacttttta tatgggacca atgtttcgct
    33421 acgaacgccc tcaaaaagga cggttacgtc aattccatca aatcggagta gaagcatttg
    33481 gatcagaaaa ccctgcaact gatgtggaga gcatggtgat ggcacttgat ttcttcaaac
    33541 agttggggat cacgcaaatc cggctagtca tcaattcatt aggtgataaa gaaacgcgtc
    33601 aagcctatcg tcaagcattg atcgactatc ttttaccttt tgaatcagag ctgagtgaag
    33661 actctaaacg ccgccttcat gaaaatccat tacgcgtatt ggacagtaaa gacaaacgag
    33721 atcaaaaatt tgtggcagaa gctccatcta tcttagatta tctaagcgag cctgctcaaa
    33781 aacattttga taccgttgtt tccatgctgg atgcattggc tgttccttat gaaatcgaca
    33841 gtaatatggt tcgcggacta gattattaca cacatacgat ttttgaaatc atgagtgacg
    33901 caccaaaaat gggggcacaa gcaacgattt gcgcaggcgg acgttatgac aatctagtgg
    33961 aagaactagg tggtccttca acacctggct tcggatttgc aatgggtatc gagcgattac
    34021 tcttgactat ggaggcagag gaagtagtta tccctgcatt taatgagttg gatgcttacg
    34081 tggtcgcact cggagacgaa acaaatatcg aagcattgaa agtggtccaa gcgattcgaa
    34141 actttggttt ttcagctgac cgcgacttca tgaaccgtaa agcaaaagcg caatttaaga
    34201 cagcagataa agcgaatgcc aaattagtat tggtgatcgg tgaagatgag cttgctagtc
    34261 aaacgattaa tgtcaaagca atggcaagcc gaaaagaaaa aagttttcca ttagaagaga
    34321 tatatgaaca ttttgatgaa gtctatgatg aaatgacttt attcgagtag gaggaaaaaa
    34381 ataaatggca caaagaacga tctattgcgg attagtttcc gaacaacaat tagaacaaac
    34441 agttacatta aaaggatggg tacaaaaaag acgagacctt gggggagtaa tctttatcga
    34501 cctacgtgat cgtgaaggaa tcgttcaaat cgtctttaat cctgaaatca acaaagaagc
    34561 atgggaaatc gcggataaat gtcgcagtga atacgtgatc gaagtgactg gggaagttag
    34621 aagacgcgga gaaaatgcag taaatcctaa aatggccact ggtgaattcg aagtgattgc
    34681 aagtgaaatc gtgattttga atcaagcaaa aaccccgcca tttcaaattg aagatgatca
    34741 agcaatcagt gacgaaatac gtttgaaata tcgttatttg gatctgcgtc gtccaaaaat
    34801 gactaaaaac atcaaattaa gaaatgcgac aacaaaagcg attcgtcatt atttagatga
    34861 taatgacttt ctcgatatcg aaacacctta cctagggaaa tctacaccag aaggcgcaag
    34921 agactaccta gttccttcac gtgtccatgc tggacatttc tatgcgttgc cgcaatctcc
    34981 tcaattgttt aaacaattgc tgatgaatgc tggattcgac cgttattatc aaatcgttcg
    35041 ttgtttccgc gatgaagatt tacgtggaga ccgtcagcct gaatttacgc aagtagatat
    35101 tgaaacaacg ttcttaacag cagaagaaat ccaaacttac acagagggat tgattgcgaa
    35161 ggtaatgaaa gaagttcgcg gtatcgaagt cacattgcca tttcctcgta tgacctatga
    35221 tgaagcaatg gctcgctacg gcagcgacaa accggataca cgttttgata tggaattgat
    35281 cgatctaagc gatacagtca aagaagtaga attcaaagtc ttccaaatgg ctcttgaaaa
    35341 cggcggtgtc gtgaaagcat tgaatgctaa aggcgctgca gatcgctact cacgaaaaga
    35401 catggatcag ttaggtcaat atgtaggaca attcggagct aaggggcttg catggctgaa
    35461 agtagaagaa gacggattga aaggtccaat tgctaaattc atgggagaag caacagaagc
    35521 aatcattaaa gcaacagatg caaaaccagg tgacttgctg atgtttggcg cagataaaag
    35581 tgaaatcgtc gctgctgcat taggtgctat ccggacacgt ttaggaaaag aattaggact
    35641 aattgatgaa tcgaaattca atttcttatg ggtaactgat tggccacaat ttgaattttc
    35701 agaagaagaa ggtcgatatg tctctgctca ccatccattc acgatgccaa aagaagaaga
    35761 tatcccgtta ttggctgaag accctgcaaa agtctatgca gaagcttatg atatcgtctt
    35821 gaacggttat gaactaggcg gaggttctat ccgtattcat actcgtgagc ttcaagaaaa
    35881 agtactagaa actctaggct ttacaaaaga acaaatggaa gaacaatttg gtttcttgtt
    35941 aacagcttta gattacggat tccctccaca tggcggtatt gcattaggat tggaccgttt
    36001 tgcaatgtta ttagccggag aagataatat tcgtgaagtc attgcctttc ctaaaaacgg
    36061 aaaagcagca gatgtgatga gtgatgcacc aagtatggtt tcgcctttac aattgttcga
    36121 attgaatatt gatgtaacga atatagaaga atagccgata aaaaggtaaa aaggatttgt
    36181 cgaatacgct tcttttgaca accttgattt aattgaaaaa cactttataa tgaagccata
    36241 acatataaag cggggctttt ccatgaaaaa aacattcgaa aattgggctt atcatgatta
    36301 cacgaccaag ctttcggtat ctatcgttta tgcgttgatc gcatcagtcg cattgaattt
    36361 cttttatcag ccaggacata tttattcaag tggtattact ggtctagctc agattttgac
    36421 gactttgtca aaatcactga taggattcga agtaccggta tcaacaactt tgtatttact
    36481 gaatatacct ttgtttgtat tggcgtggtt aaaaatcggg aagaagttta caattttcac
    36541 ttttatcaca gtcactttga cctccctttg tatccatatt ttaccggaaa cagtcctatc
    36601 taccgatcca atcatctgtg cgttgtttgg gggggctgtg atgggactag gtattgggct
    36661 agcgttgaga aacggcctat catcgggcgg tctagatatc gtgagtatca cgattcgtaa
    36721 aaaaacagga aagtcgattg gttctatttc gatctacttc aatgcattga tcgtttttgt
    36781 tgccggaatt ctatttggct ggcaatatat gttttacagt acgttgtcta ttttcgtcag
    36841 tggaaaagta actgatgtcg tttatactaa acaaaagaag atgcaggtaa tgatcgtaac
    36901 gaaacatcca gaaaaagtag taaatgaaat ccaaaactgt atgcgtagag ggatcacgat
    36961 catcaatgaa gcagaaggtg cttataagca tgataagcag acagtattga tgacgatcgt
    37021 tacacgatac gaactgcctc tacttagaga agcaatgaaa gcttctgatc cacaagcttt
    37081 tgtaagtatt gcagaaaacg tccagatact tggtcgtttc tatgaggatg gcatttagat
    37141 cgagtttgac aaaaattcat gaaatactgc tgtagacaga cgttcgtttg tctgcagttt
    37201 ttttctacca tagtagaatc agcaaagaaa aactgctttt ttatttgaaa ttttcttaaa
    37261 gttgaaaaaa agcatgttta tttttttaaa tttatgggat gatagaaaaa aatagtagag
    37321 aaaggacacg attctacttg ctattttcac ttaatacccg tatattacga atgaaaaaag
    37381 tttatgaaaa gttggaatat tgaatggaaa aacaaaaaag gttaaaaaac agccgctaca
    37441 tcactgggtt tgatgggctc cgttcattag ctgtggtggg tgttatcttc tatcaccttc
    37501 tgccaactag tatgaaaggc ggctatttgg gcgttccgat ttttttcgtc gtatctggtt
    37561 atttgatcac ggatctgctg cggcaggaat gggaacaaaa tggaaaaatc aatatttggc
    37621 aattttatgt cagaagaatg aagagactct atccggcact tgtttttatg cttgtgacag
    37681 cttcggctta tatcactttg tttcagcgag ggctgttaaa caatcttcga ggagttgtgg
    37741 tcagcagctt actgtatgtc aacaactggt ggcaaatcaa taacggttta tcttatttcg
    37801 atcgatttgc taatgagtca ccgtttacgc atatctggtc tttagcagta gaaggtcaaa
    37861 actatttggt ttggccgatc ttgtttgtat tgttgatggt atttgttcgt aaaagaaaat
    37921 ggatctttta tactgtgtta ggtacatcct tcctctccgc catcttgatg atgatcttgt
    37981 atacaccagg aggcgatcca acccgtgttt attacggtac cgacactcgt ctgttttcta
    38041 tatggttggg aagtgcgctt gcgtttgttt ggccaagtac gcgtttaaag aaaaacatcc
    38101 cgaaacaagc gaaaaatatt ctgaatatcg ctggaggaat ctcgcttttg gcactgattc
    38161 tttgcttctt ctttttagat gatcattaca atttcatcta ttacggagga atgctcctag
    38221 tgagtctgtt atgcgtagtg ctagttgcag tgacagcaca ccccggagca agtttgaatc
    38281 gatggctgac aaatccagta tttacttgga ttggcaaacg aagctatggc atttatctgt
    38341 atcagtttcc agttatgatt ttttatgaag caaaggtgac tgatattgcc gatcatgttt
    38401 tgttgcatac tttagtggaa atcacattga ttctaatcat cagtgaactt tcttatcgat
    38461 atgtggagcg tccgctagca cgttttgatt atggtcgtac attccaagtg atgaaaggat
    38521 ggttcagcaa accgatcatc agtaagagca aaccatgggt tgttccaggc acattacttg
    38581 tagcaattgc actcgttggt ttcgttacgg caccgaaaaa cgctgtaaca gccgatcaaa
    38641 aacaactgca ggcaaaaatc gctgaaagta aaaaattagc agaagaaacc agaaacaatt
    38701 cagaaaacac cactagttcg gttgaccaag cgatccttga taagtacgag ttgtccaagg
    38761 cacaagggaa taaggcacaa aaattagaac taacggcatt tggtgattca gttatgttgg
    38821 atgctgcaac ggatctacaa gaactttatc caaaagtagt ggtagatgga gatgtcggac
    38881 gacaattata tgccagtgta ccgtacattg aaaaattaaa ggaacaagat ttgttgaaag
    38941 acactgttct gatcggacta ggaacgaatg gctcattctc ggaagctcag tttgatagtg
    39001 tgatggatgc aatcggtgat cgaaaagtat actgggtcaa tgtccgagta ccgacgaagc
    39061 gctggcaaaa tgacgtgaat gcgatgcttg aacagatgac gaaaaaatac gacaatatga
    39121 cgctgattga ttggtacagc tacagtaatg accatagtga ctggttttat gatgatcaag
    39181 tccatccaaa tcctgaaggg atgacacatt acattcactt agtcagcgaa gccttgctag
    39241 gaggaagttc aagtacgaac agcagtgaga caaacgtgac agaatgatag ctacaaaaaa
    39301 cttcaggcaa aaacatttgt ttttgcctga agttttttta tatttaccgt gtttttaaca
    39361 ttcatataga ttcaggaagc aaaacggttg agtgtgtctg taaataacgt tctttttgtt
    39421 ttcgggtgag ttttttgaat gcatcttctg cttttgttgc ttcgcttctt gaagaaaata
    39481 cttctgcatg gatcatctta acaggtcttc ttttttgaag acgagtatat ttagcaccag
    39541 taccattgtt atgttcctct aatcgtctag aaagatcggt tgtatatcca ccataaaatg
    39601 tctgatcttt acagaataat acgtaaaaat aatgttcatt tgccatagag catctcacga
    39661 atctctgcag tgtattctcc atccatattg taagtgatca atggtggagc gactttgaat
    39721 ccgtctaatt tgccttcttt gattccttca actaacaaaa tatttgcctc ttttccaggt
    39781 tttgggtaaa caaattggat ccttttaggt gcaattcgat agtcttccat gacctgaatg
    39841 atttctaaga accgatcagg acgatggacc atagtaaagc gtccatttgt ttttagtagc
    39901 ttacttgaaa tatcgatcac ttcttctaac gtcgtatgaa tctcatgacg ggcgattgcc
    39961 aggtgtttat tagggttcgt tttatttgtt ggcagccctt tgaaataagg tggattacag
    40021 acgactaagt cacaagaatt atgtcgaacg acagataagc tgtctttcag atcgatggtg
    40081 tggacagtca tttgttcatt taaatggtta agcgcgatac tacgtcttgc catgtctgct
    40141 agacgaggtt gaagttcgat agcgtcgata gccgcctggg ttcgtttact taaaaaaagt
    40201 ccaactgcgc catttcctgc acaaaggtca acaatcttcc cttttttagg tacacgggaa
    40261 aagtgagcaa gcagcacagc atctaaagaa aaagaaaaca ctttggaact ttggataatc
    40321 ttgatatctt ctgcgtataa ttggtcgatt cgttcgtctt cgtgaagcat ggattccctc
    40381 caaaatgaaa aaaatagtcg tctgtattat actattttta ttaaaaagaa ccaaggtggc
    40441 ttgtcaaacc tctatttttt atgcatacta tgaccataag aagggaaagg aagagactat
    40501 gttttatcga tttatgcgcg gggtagttcg agtactatta gccttagtta atggaaatgc
    40561 ccgctatgag aataaagaag ttttaccgca agatgagaat tacatactag ttgctcccca
    40621 tcgtacttgg tgggacccat tatatctggc ggtagctgca gcacccaagg aattcagctt
    40681 tatggcaaaa gaagaactgt tcaaaaatcc agttctgcga tttatattaa ctcgatccaa
    40741 tgcttttcca gttaaaagag aaaatccagg accaagcgtc attaaaacac ctgtaaagat
    40801 tttgaaatcg acaaatttga gtttgatcat gtttcccagt gggacaagac atgctacaga
    40861 acttaaaggc ggaatggcat tgatttccag aatgtctaaa gcgaaaatcg tgccagctgt
    40921 atatcaaggt ccgttgacac ttggggatct gttcaagcgt cagagagtga cagtgcgttt
    40981 cggcgaacca atcgacttat cagatatcaa gaaaacggat aaagaaggta tggcggagat
    41041 cgaacgacga acacaagcag cttttgacaa attggacaaa gaagtgaacc ctgatttcaa
    41101 atatgaagtc aaaaaaaaat aaaatgataa gtttatgcgt gggcggtctt ttgaatctac
    41161 tgtcctgttc agttcagact ttttcaatga aaacgaggga gagacgtcaa gtctgccctc
    41221 tttttgcgtt ccaatatgta aaatagaaga caggtgtcat gtaatggatc aaagtgttat
    41281 aataattaga aggacatttt ttgaggtgat tgaatgcgtt ttttacatac agccgattgg
    41341 catatcggaa aaaaactaca aggttatgac ttgattgaag accagcgtca tgtactatct
    41401 tctatactag atatcgcgaa agaagaaaaa gtagaggcaa tcgtgattgc cggagactta
    41461 tatgatagga gtgtcccagc tgttgatgcc gtagaattat acaatgaatt gatggtcgat
    41521 tggaatttaa aggaaaagat cccgattttt gctatatcag gaaatcacga tagcagtacg
    41581 agactttcag cgggtacgcc atggttcagt tattcgaact attttttgcg aacacagcta
    41641 gcagaagcat ttgaaccagt cgagtttaaa aacgtacaat ttttctttct tccttacttt
    41701 gaaccaattg cagcgagatt atattttgaa gatgacaata tccgaacgat caagcaagcg
    41761 gtagccaagg tgatagataa aatgcaggag ctatttgatt cagaaaaaaa acaagtattg
    41821 attagccatt tttttgtaat gggaagtgta aaatctgatt cggaaacgaa aatcgaagta
    41881 ggcgggttgg attcgatccc tggtgaacta cttacaccgt ttgattatgt agcactagga
    41941 catttgcata atcaagctgc tttgagacag gataatgctc gctatagcgg ttccccattg
    42001 aaattctctt tatctgagat caaccaaaaa aaaggcgtat ggatcgttga cctgacaaca
    42061 ggcctggagt tgaattttag ggagatcgaa cccctgcatg atattgaaga aataacagcg
    42121 agtttcaaag aattgctgga tcctgccttt tatcagtccg tcaatcggga gaattacatc
    42181 tatatctatc tcactgatag aacgatcatc ccgaatatga tgaaccagct tcggcaaata
    42241 tatcctcgta ttttggggat tgaacgggca aacggtcgag cagaacaaac aaagcgcaaa
    42301 cgaaaagtac tagaaatgaa agatcctcaa aaattagcag aagcattttt tagtgaaatg
    42361 acggaagagc agctaactgc aaaccaagaa aaatggctcg gagaaacgtt gcaagaaatc
    42421 atggaaatgg aatgatcaag aatgaaaccg aaaaaattac aacttaaaaa ttttggtcct
    42481 tttatcaatg agaccgtaga tttttcacgt ttgacagaag caccgctttt tttgatcagc
    42541 gggaagacag gagctggcaa aacgacgatt tttgatggga tcaccttcgc actttttggt
    42601 gaaacttccg gaagacttcg ctctggaaaa gagatgcgtt ctttgtttgc tacaccagac
    42661 gaagaaacaa gcgtgacttt ttcttttgaa catcagcaga tgagctatga aatcgaacga
    42721 aaaccagaac aagttttagc aaaacgaaaa ggaaacggaa caaaaaaaca ggcagccaaa
    42781 gtaacgttga ccatttttga tgggaaagga aaagaaataa aacaaatcac aaaacgtaca
    42841 gaagcagatc agttgatcag agaactgatg aatctcgatg cgaaacagtt ttctcaaatc
    42901 gttttactac cccaaggaga attccgtaat tttttggttt catcaagcag tgataaagag
    42961 actgttttac gtcatttatt cggtactcaa tttttccaac agttcaatga gcgattaaaa
    43021 gaaaaagcaa aacaacagca acaaaaattg gatcatttag agcaagagct gcatcttctg
    43081 caaaaaaggt ttgttttagt caaagaagag gaaccagcag cagggtttga agccgtttta
    43141 gcccaatgga caaaccatca ggcaatactc gaaaaacaaa tcacggaaga aaaagaagct
    43201 ctaacggaac ttcaggaaaa acaaaagcaa ctagaaaatt cctactatcg ttttgaaagc
    43261 ttgaaaaaaa gttatgaaga aaaaacgata ttaataaaca agcaaaagga attagctgct
    43321 caaaaagacg aaatagagga aaagaaacga tggattgggt attatgaatt tacgcaacga
    43381 ttagtcgaac caatccagcg cctacaagaa ataaaagttg aaaaaataga actgtcacaa
    43441 aaaagtagtg agcaaacaaa gaaactagcg atgactacgc agaattatga agagtggcaa
    43501 aaaaatgaag caaaccgcgt acgccgacaa acaaaaatca aagaagctgc tcaaaaactg
    43561 caaaacaaac agatgatggt gccaattgtc gaatccttac gtagtaaaaa acaagacgca
    43621 gaacaattaa caatttcatt ggataaagaa gaattgaatg ccgagaagct gtatcaaaat
    43681 ctcgctatct atcaggaaag aactcgaatt cttcaagaaa atttactgga tcaggaaaaa
    43741 ctgcaagaag atcgtcttgc ttttggacag ttaaaatatc tagatgaaaa gcagcaagaa
    43801 atagaaaaac aaaaagcaaa agacactggg tcgctcgcac agataagaga agaactcaaa
    43861 aaagcaactg aagaaataac aaaaaatagt cagctattga aagaacagac agaacaagca
    43921 gaacagctga aaagccaatg ggcaaagcag caaattgctc gtcttcagct ttttttgcgt
    43981 ccaggggagc catgtcttgt ttgtggatca acggaacacc ccatacaaca attaaaacac
    44041 gaacagccaa ataaggaaga tatcttaaaa gtagaacaac aactggcaga agctgagtct
    44101 gctgtagaaa gaactcagca aatcattgcc aaaagtgaag cacatgaaaa taatcttcaa
    44161 caacaattat cagatttaga acaaaaaatc atggcaacga atatacaatt ggctgaacaa
    44221 gaagaacact tgcaacaggc tttgaaaaag gcatttcctg agataacaga acaaaaaaca
    44281 aaaacaactc ttgtagtaat cgaacaatct ttagatagac gaaaaagcaa gatggccaat
    44341 gcaagaatag agctggaaga aataagacaa caagcagaaa agctcgaaca acaattagct
    44401 gtctacaaac agcagcagac agaaaaaaat aatcagctcc agcaactgca aggagaaatc
    44461 aaaagtttag aacaacaatt gcaagatacg gatagtaact gtttagaaga ggagatacga
    44521 cgacttgaaa aacagttaga agaagactcg aatcaactag ctgttgagca gaaagcagga
    44581 gtgcaattgt tgcaagatct tcatacgctt cggacacagc ttgatcttca acatgaacaa
    44641 atagaaaaaa tagatgaaaa aatagaaaag gaacaagaaa gaatagctaa aaagttagaa
    44701 agccagtcgt atttttcaac aattgaagaa atctgtgcat ttgcacaaca gcaagcacaa
    44761 tataaagaaa accaacgtac agttacttct tatcatgaag agcaattagt gacagctgac
    44821 cggctgcgtc aattagaaaa tatccagcta actgagacat tcccagatac agaagagatc
    44881 agccaacagt tagaagaaat aagacgtatg atcgaaataa agcaagaaaa gatatacggt
    44941 ttacaagaac aaaaaattag taatcaaaaa atctacgatg aatgtctttc gatttatcac
    45001 acaagccaga cgaaattaga tgagcttacg caattacagc agctttcgca aacattcaac
    45061 ggagaaaatc ctaagaagac tagtttggaa cggtatgtgc ttcaggttta tcttcaagaa
    45121 gtgttacaag tagcaaatga tcatctccaa cgtttaacga aaaatcgtta tcaatttgag
    45181 ctagcggata ccattggcag ttatcgaggg aaaaccggat tagaaatcaa tatctatgat
    45241 gatcaggcag gtatgtctcg aagtgcgcat accctgtctg gcggtgagag ttttattgca
    45301 gcactttctt tagcattagc tcttgccgat gtgatccaga cacaagctgg tggtgtagca
    45361 atcgatgcat tgtttattga tgaaggattt gggtcattag atgaagaagc tttggaaatg
    45421 gcaatggaag ctttagaaac gatcgaaagt gaaggaagaa tgatcggcat cattagtcat
    45481 gtaagagaat tgaaagaacg agtattacaa caaataagaa ttgatacaga aggtagcgga
    45541 caaagcaaaa tacgctatca acttggataa aaagggagat tggtaactat gaaatggagt
    45601 attccagaaa aagtcattgc acgtggacgt caatacttgc aagaaggccg agtgttatcg
    45661 gttgtccctg accaagaaga aaacgtgtgg catgcagaag tcttgggcag tgaattgtat
    45721 atggttgatt tggatggtac agcaaaagag atagattact gccaatgtcc ctattgggag
    45781 gaacataaat attgtaagca tacagttgct gtggaacttt atttacggtc gaaaaatcta
    45841 tctcgtatca tgaaaaaaga ccagccaatg ctagaaccgg taaaagcaac aagtgaggga
    45901 gaaatcattg caaaaggttt ttctcgtcta aaaacacaaa acaatcctca agcggtgcaa
    45961 cctttgatta ttgaatacca agtagaaacg attgagacta atcagtatca cccagagctt
    46021 tctattttgg cactttactt gcgtatcggt tcattggcat caccgaaaaa aacatatatc
    46081 gtaaaaaaca tctatgattt tttacaagct tacacagaaa aagaaactta cactgttaat
    46141 aaacagtacc agtttcgtct agataaacgg gcgtttcaaa aagaagatca aaaaatactc
    46201 acgcatcttg caggaagtgc tcagacgcat caattattag gtacgaacgg cttacaagtc
    46261 aaaggaaaac tagataaaag atatctgctt ctcccagtag aacaagcccg cttcctgtta
    46321 gagcagatga atcagactac acgtttgcat ctgcagataa atgaacaaaa cgatcaagga
    46381 atacaatttt ccgatcaaaa caaaccttta tcattcaaag tggaaaaatt tggagaagat
    46441 tatttgctgc aagcattgaa tgattttgat tttttcttta tgcattatca gtggggagtt
    46501 atccaaggga cattttattc tctgacgaaa tatcaacaaa cggtttatca aacttggaaa
    46561 caattgattc gccgattgga gaaaccagaa gtagtgttca agaaaaaaga attaccgtct
    46621 ttgtttaaag agattatccc tctgctgtca gagatcggcg acgtagctat tgcccctgca
    46681 gtagaagagg aagtagcaga tatccccgtc gaatttattt tctttttccg aaaagcaaaa
    46741 ggaaaaatcc aagctcgagt cgatttcgtc tatggcgaag tcatctattc gactgacccg
    46801 aagcatgagg tctcgacaga acaggctttt caggtgttac gtgacacagt agaagagaaa
    46861 cgagtacttg atctattcag aatgtatcgc taccagaaaa atgaaactgg ttatgaacgt
    46921 ccattgccaa gcggggaaga actatatgcc tttttccgca cagaactatc tgtcttccgt
    46981 cagtttggtg aagtcagact tggaagaaaa ttgcgagagt tgtatttaga tgcacataaa
    47041 catcaaccga aaatcgaagt aaccgagacc ggttcatggc tcgatattcg attcgatgtg
    47101 acagggatag aggagcaaga aatcgatcat gtcctgcaaa gcttgctgag aaatgatgct
    47161 ttttatacat tagaaaatgg tcaggttctt tcttttgatt cagaagaatt tcaacaaaca
    47221 agccaagtgt tgcaacaatt acgggaatcg atacgtacag aagaaggaac gatccatgta
    47281 cctaaaaatc aaggattgat cattcaaaat caattggaaa aaagcaatgc tacttttagt
    47341 gaatcgttcc aaacgatggt acaagatctc attcatccag aaagatacca ggcacagctt
    47401 cccaaaggat tgaatgcaac gatgagagat tatcaaaagc aaggttttcg atggttgaag
    47461 atgttaggtc attatcagtt tggcggtatt ttagccgatg aaatgggact tgggaaaaca
    47521 ctacagacaa ttgccttttt actttcggaa aaagaagaac ggaaaagctt ctctgctttg
    47581 atcgtagcgc ctgcgagctt aatttacaac tggcaggcag aggtgagaaa attcgcccct
    47641 tctttaagta tccaagtaat caatgggaat aaaaaagaac gtgaagaatt actggctaaa
    47701 gacacagata tccgagtgac ttcttatgcc agcttaagac aggatctagc taattatcag
    47761 tcgcaaaaaa ttgattattt gattcttgat gaggcacaaa tggtcaaaaa tagcagtacg
    47821 aaaactgcac aagcattgag agaattagct gtacctcagc ggtttgcact gagtgggaca
    47881 ccaatcgaga ataatttaga agaattgtgg tcattgtttg caacgattat gccaggattt
    47941 ttcccgacta aaacaaaatt cagggaactt gccgcagaag aaattgcgca aatgattcga
    48001 ccgtttattt tacggcgaga taaacaaact gtgttgaaag acttgccgga aaaaacagag
    48061 atgaatctat attctgcact tacagaagaa caaaaaactg tttatttagc ttacttgcgt
    48121 caaatgcgtg aagaaatcac atcaatggat tctgaagcat tcaagaaaaa ccggatcggg
    48181 atacttgctg gtctgacaag actgcgacaa atttgctgcg atcctcgtct gtttattgaa
    48241 gattaccaag gcggttccgg caagctcgaa caagtaaaag atctactggt agctgccaaa
    48301 gagaacaaac gaagagtttt gctgttttct caatttacag ggatgctgac tatcttgcaa
    48361 gaagagttag ccgagctagg gatctcgaca ttttatttaa gaggcagtac gaaaccacaa
    48421 gatcggttaa gtatggtaga cgcatttaat gctggcgaaa aagatgtttt tctgatttcc
    48481 ttaaaggctg ggggaactgg attgaatctc acgggagcag atacggtcat cttgtatgac
    48541 ctttggtgga atcctgccat tgaagaacaa gctgctggac gtgcacatcg gatcggacaa
    48601 aaaaatgtgg tagaagtgtg gcgtatgatc tctgaaggaa cgattgagga acgcatggat
    48661 tcactccagc aagaaaaacg cgaactgttc caaaaagtga tccaaggtaa tgaagaacag
    48721 ttgacgaaac tgactgaaga agacatccgc atgattttaa gtgtaggaga aatcgaggag
    48781 taagagactc gaaaaagcgt tcagtaatgg gtaagaaagc ctttctaaag aagcgattgt
    48841 ttatttgatt taaaaaatag caaggaggct gggattccat tttatcccag cttccttgct
    48901 atcatttttt tatttctatt tttaaaggtt atacaaagat tgtcttcatt gtctctaaca
    48961 gctgatcaaa agaagaaatc tcgatatcgc aaattggagt ctgttcaatt ttgcgttctc
    49021 gatggttgaa ccaaagcgct tgccagttag cactttttgc acctagaaca tcattgtcgt
    49081 aattatcgcc aacgtataag gtctcttctg gaagcatatg aaattctttc tcagctagct
    49141 gaaaaatctc tttttctggt ttttggaaac ctgttgcttg agaaatgagc atattgcggg
    49201 aaggaatcca attgttcaat tgaagttgct tcagtttttt tgtctgatgg tcagtcggcc
    49261 catttgtgat aattcctaat gggacattct tttccttcaa gaaatcgagg gtttttttga
    49321 cctcctcatg caagcagata ttatcaagtt cttcctcgta aattttttga aataagaggc
    49381 catcttcttc cgtaatatga gggtagtcta aatcttttag tgattggctg atccgatgtg
    49441 ctcgcatata ttctagtgtc caatctcctg ccattacttt tgggaagttt tcatcactat
    49501 gatggcggaa ccggatatat agtggatgca tatctgcatc tgaaacaagc ggaacgactc
    49561 ttttgaccgc gtttcgaaat ggctgctgct gatcatacat cgtatcatca acatcaaaca
    49621 caactgattt aatcaaaatt ttacacgcct ttcggttttc attgattttt tcacaaatcc
    49681 ttgactattg taacagaaaa aattgtctaa aaaagctgat aaataaaggt ttttcttact
    49741 atatttctat ttccttgaca gaataaggtt tttatatttc agtttttttc actaaaaatc
    49801 gtgaaacacg tcaataagtt tgatgaacaa attaatcatt atggtagaag tcgattgatt
    49861 tattttaatg caatctctgt ggaattttcg aaagatttat ttttttaata cagtctcaat
    49921 ataactattc aaacataaaa taacgttatt tgatttattt gtttatagta aattttattt
    49981 agtaatc
//
LOCUS       DAXWLB010000004        48038 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXWLB010000004 DAXWLB010000000
VERSION     DAXWLB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.004"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            16..903
                     /locus_tag="ACGE4H_000163"
     CDS             16..903
                     /locus_tag="ACGE4H_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8363614.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1043..1969
                     /gene="cysK"
                     /locus_tag="ACGE4H_000164"
     CDS             1043..1969
                     /gene="cysK"
                     /locus_tag="ACGE4H_000164"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFF8363615.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2205..2651
                     /locus_tag="ACGE4H_000165"
     CDS             2205..2651
                     /locus_tag="ACGE4H_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8363616.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2923..4275
                     /locus_tag="ACGE4H_000166"
     CDS             2923..4275
                     /locus_tag="ACGE4H_000166"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFF8363617.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4446..4733
                     /locus_tag="ACGE4H_000167"
     CDS             4446..4733
                     /locus_tag="ACGE4H_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8363618.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4959..5402
                     /locus_tag="ACGE4H_000168"
     CDS             4959..5402
                     /locus_tag="ACGE4H_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8363619.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIRDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5408..6592)
                     /locus_tag="ACGE4H_000169"
     CDS             complement(5408..6592)
                     /locus_tag="ACGE4H_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8363620.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6860..7558
                     /locus_tag="ACGE4H_000170"
     CDS             6860..7558
                     /locus_tag="ACGE4H_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8363621.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7565..8242
                     /gene="nth"
                     /locus_tag="ACGE4H_000171"
     CDS             7565..8242
                     /gene="nth"
                     /locus_tag="ACGE4H_000171"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFF8363622.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8368..8901)
                     /locus_tag="ACGE4H_000172"
     CDS             complement(8368..8901)
                     /locus_tag="ACGE4H_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363623.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9198..11579)
                     /locus_tag="ACGE4H_000173"
     CDS             complement(9198..11579)
                     /locus_tag="ACGE4H_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8363624.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITERTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11569..12216)
                     /gene="recU"
                     /locus_tag="ACGE4H_000174"
     CDS             complement(11569..12216)
                     /gene="recU"
                     /locus_tag="ACGE4H_000174"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFF8363625.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12280..12819
                     /locus_tag="ACGE4H_000175"
     CDS             12280..12819
                     /locus_tag="ACGE4H_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFF8363626.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12909..13328
                     /gene="gpsB"
                     /locus_tag="ACGE4H_000176"
     CDS             12909..13328
                     /gene="gpsB"
                     /locus_tag="ACGE4H_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFF8363627.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13350..13717
                     /gene="rnpB"
                     /locus_tag="ACGE4H_000177"
     ncRNA           13350..13717
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGE4H_000177"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13926..15092
                     /locus_tag="ACGE4H_000178"
     CDS             13926..15092
                     /locus_tag="ACGE4H_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFF8363628.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15138..16634
                     /locus_tag="ACGE4H_000179"
     CDS             15138..16634
                     /locus_tag="ACGE4H_000179"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFF8363629.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16715..17398
                     /locus_tag="ACGE4H_000180"
     CDS             16715..17398
                     /locus_tag="ACGE4H_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8363630.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18043..19233
                     /locus_tag="ACGE4H_000181"
     CDS             18043..19233
                     /locus_tag="ACGE4H_000181"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFF8363631.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19335..20288
                     /locus_tag="ACGE4H_000182"
     CDS             19335..20288
                     /locus_tag="ACGE4H_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFF8363632.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20357..21706
                     /locus_tag="ACGE4H_000183"
     CDS             20357..21706
                     /locus_tag="ACGE4H_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8363633.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21889..22212)
                     /locus_tag="ACGE4H_000184"
     CDS             complement(21889..22212)
                     /locus_tag="ACGE4H_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363634.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22341..23504
                     /locus_tag="ACGE4H_000185"
     CDS             22341..23504
                     /locus_tag="ACGE4H_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8363635.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASRQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23574..24923
                     /locus_tag="ACGE4H_000186"
     CDS             23574..24923
                     /locus_tag="ACGE4H_000186"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8363636.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24968..25399)
                     /locus_tag="ACGE4H_000187"
     CDS             complement(24968..25399)
                     /locus_tag="ACGE4H_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8363637.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25787..28429
                     /gene="alaS"
                     /locus_tag="ACGE4H_000188"
     CDS             25787..28429
                     /gene="alaS"
                     /locus_tag="ACGE4H_000188"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFF8363638.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28629..29225
                     /locus_tag="ACGE4H_000189"
     CDS             28629..29225
                     /locus_tag="ACGE4H_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8363639.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29347..30495
                     /locus_tag="ACGE4H_000190"
     CDS             29347..30495
                     /locus_tag="ACGE4H_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8363640.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30533..30946)
                     /locus_tag="ACGE4H_000191"
     CDS             complement(30533..30946)
                     /locus_tag="ACGE4H_000191"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFF8363641.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31025..31456
                     /locus_tag="ACGE4H_000192"
     CDS             31025..31456
                     /locus_tag="ACGE4H_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFF8363642.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31555..31788)
                     /locus_tag="ACGE4H_000193"
     CDS             complement(31555..31788)
                     /locus_tag="ACGE4H_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8363643.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31971..33641
                     /locus_tag="ACGE4H_000194"
     CDS             31971..33641
                     /locus_tag="ACGE4H_000194"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFF8363644.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33788..34174
                     /locus_tag="ACGE4H_000195"
     CDS             33788..34174
                     /locus_tag="ACGE4H_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363645.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34161..34859
                     /locus_tag="ACGE4H_000196"
     CDS             34161..34859
                     /locus_tag="ACGE4H_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8363646.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34864..35328
                     /gene="lspA"
                     /locus_tag="ACGE4H_000197"
     CDS             34864..35328
                     /gene="lspA"
                     /locus_tag="ACGE4H_000197"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFF8363647.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35325..36236
                     /locus_tag="ACGE4H_000198"
     CDS             35325..36236
                     /locus_tag="ACGE4H_000198"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8363648.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36471..37010
                     /gene="pyrR"
                     /locus_tag="ACGE4H_000199"
     CDS             36471..37010
                     /gene="pyrR"
                     /locus_tag="ACGE4H_000199"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFF8363649.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37024..38307
                     /locus_tag="ACGE4H_000200"
     CDS             37024..38307
                     /locus_tag="ACGE4H_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFF8363650.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38320..39246
                     /locus_tag="ACGE4H_000201"
     CDS             38320..39246
                     /locus_tag="ACGE4H_000201"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFF8363651.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39265..40545
                     /locus_tag="ACGE4H_000202"
     CDS             39265..40545
                     /locus_tag="ACGE4H_000202"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFF8363652.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40550..41626
                     /locus_tag="ACGE4H_000203"
     CDS             40550..41626
                     /locus_tag="ACGE4H_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFF8363653.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41631..44816
                     /gene="carB"
                     /locus_tag="ACGE4H_000204"
     CDS             41631..44816
                     /gene="carB"
                     /locus_tag="ACGE4H_000204"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFF8363654.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44870..45646
                     /locus_tag="ACGE4H_000205"
     CDS             44870..45646
                     /locus_tag="ACGE4H_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFF8363655.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45643..46575
                     /locus_tag="ACGE4H_000206"
     CDS             45643..46575
                     /locus_tag="ACGE4H_000206"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFF8363656.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46572..47288
                     /gene="pyrF"
                     /locus_tag="ACGE4H_000207"
     CDS             46572..47288
                     /gene="pyrF"
                     /locus_tag="ACGE4H_000207"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFF8363657.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47295..47924
                     /gene="pyrE"
                     /locus_tag="ACGE4H_000208"
     CDS             47295..47924
                     /gene="pyrE"
                     /locus_tag="ACGE4H_000208"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFF8363658.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
BASE COUNT        15812 a         8384 c        10335 g        13507 t
ORIGIN      
        1 tgaggaggaa tttgaatgca agagctgaag acccgcagtg cccggcacca tagacctaaa
       61 agaaaaatgg tgatcaataa aaaagcgatt gctcctttaa ttggaacgag tcttttagtt
      121 gggccgatga tggttacagc ggtaccgcaa catgttcatg cagatgaaca aggatctcaa
      181 acagtcgctg atcctcaagc gtttatcgat caaatcggct ggtctgcgtc agaagttgct
      241 gcaagtaatg acttgtatgc ttcagtcatg atcgctcaag cgttgttgga aagtgcatat
      301 ggcacttccg gattagctag tgcgccaaat tataatctat tcggtgtgaa aggaagctat
      361 aacggacagg tcgtttatat ggcaaccaaa gaatatgtaa acggacaatg ggtaacgatg
      421 aatgagccat tccgaaaata ttcttcttat tgggaatcct tccaagacca tgcgaatgtt
      481 cttcgttcaa ccagtttttc cacgggcgat tatcattatg ctggcgtgtg gaaaagcaat
      541 acaacaagtt tttatgatgc aacagcgtac ttgacaggtc gttatgctac cgatccaaat
      601 tatggggcca aattgaactc attgattaat acttataatt taacccgttt tgatacgcca
      661 tcaacaggtt cggctacaac aaccacaagt acaacaacaa cgactactac gacgacttca
      721 acgtcatcat cttcaacaac agatgtaaat acaaataatg gtcagacata tacagtcgtg
      781 tcaggtgata cgctatggga tattgcacaa aaatttggca tgtctgttga tgatctaatg
      841 gcgaaaaatg gcatgtctat gtccagttat tctttattag ttggtcaaga actaaacgtt
      901 tgatttagat aaaaaggcaa agaaattacc atatatggta tatctttgtc ttttttttgc
      961 tagtcaaaga cgaatcactc gccataaatg aacagattgt attatgataa gtaagaagag
     1021 caaagacaag gaggaattga agatgacagc tatctataac tcagtcaccg aattgattgg
     1081 caaaacgcca atcgtcaaat taaataaaat cgtaccagaa gattcagcag atgtttttgt
     1141 aaagcttgag ttttttaatc caggaggaag tgtaaaagat cgtattgctt taagtatgat
     1201 agaaaaagca gaacacgatg gtctactgaa accaggtgat actatcattg aacctacttc
     1261 tggaaataca ggaatcggat tgtcgatggt aggagttgca aaaggatata aagtaatcat
     1321 cgtgatgcct gagacgatga gtatcgaaag acgtttattg atgaaagggt acggcgcaga
     1381 gttgattttg actcctggtg cggatggtat ttctggatca atcagagaag cagaacgttt
     1441 ggcaaaagaa aacggctact ttttaccatt acaatttgaa aatgaagcaa atcctcttgt
     1501 gcatgagaag acgactggtc ctgagataca tcaagcattt ggagtaaatg gcttggacgc
     1561 gtttgttgca ggtatcggta caggaggaac gatcactggc gctggccgag aattaaaacg
     1621 tgtgtatccg aaaatcgaat taatcggggt agaaccagca gaatctgcta ttttagaagg
     1681 gaaagaagcg ggacctcaca aaatccaagg aatcggtaca ggttttgttc ctaaaacact
     1741 ggatacttct gtgtatgata aagtcctttc tattagtgga gacgaagcga tggaaacagc
     1801 tagagaagtt gggcgaaaag aagggatttt agtaggcatc tcatctggcg ctgcaattgc
     1861 tgccgcgcta aaagtcgcaa aagaattagg aaaaggcaag aaggtattag ctgtggttcc
     1921 cgacaatggt gaacgttatt tatctacagc cctttaccaa gagatataag gcacttggtt
     1981 tttctccaga taggtttatg taaaaaactt ttaaagatga aaagaacaca caaaaattta
     2041 tgatgagaaa aattatttga atagagacgt agaatagtga acgcgactgt tttacgtctt
     2101 ttttcattaa aatacaataa aattgaattt tcttttcatg ttaagtggat tttaaaatgt
     2161 aaaaatagta tgataagacg agaataacaa cgtggggtgt ttacatggaa aataagttag
     2221 tcgaagaggt tatcgaaaag ttaaaaaaag agaacattcg aatcacacct caacggtacg
     2281 cggttttaga atatttgatt gaacatcatt cccatccaac ggctgatgag atttataaag
     2341 cacttgaaca tcggtttcca aatatgagtg ttgcgactgt ttacaataac cttcgcttat
     2401 ttacttcgat tggatttgtt caagaaatga agtacggcga tgcttctagc cgatttgatt
     2461 tcagttcaaa gcgtcattat catgtaattt gtgaaaactg cggtaagatt gttgattttc
     2521 attacccagg gcttgaagat atagaaatgg ctgcaagcag actgactgat tttgagatta
     2581 atgaacatcg gttggaattg tatggactct gtccggattg taaagagtca gcaaaaaagg
     2641 cgcaaatgta aaaacagttt ctacaagtgt tatacctaaa aaaagctagc tgtgtaaaaa
     2701 aaacgctagc ttttttattt ttttgtcttt tattcacaat cttagatata aaagtgaata
     2761 ttattaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa tgaaactaca
     2821 aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt tctgttatag
     2881 tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt agtagtaatt
     2941 ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca tccagaagca
     3001 gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg tatcgcactt
     3061 tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc tgaagaatta
     3121 tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga tacagaaaat
     3181 aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc ttatgataaa
     3241 ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat cgatgcgaaa
     3301 aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca agcgaaagaa
     3361 gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt agaagctttt
     3421 gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt gaataaatat
     3481 ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg tgttactctt
     3541 gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt gaaacaagta
     3601 gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg cttccgtcca
     3661 aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat caaagtgaat
     3721 gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc agtagtcaac
     3781 tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt tcgtcaaggt
     3841 ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac tcaaggaact
     3901 tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa agaaagcgca
     3961 actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg tccagagttc
     4021 atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac gaatcgtatc
     4081 gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac catgtcttta
     4141 gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt cttccaacca
     4201 cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct aggcgaaatc
     4261 gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc tctgtctttt
     4321 tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata agcagcaaag
     4381 ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga aaggtgagaa
     4441 agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat tataaaagtg
     4501 cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc tctcagttga
     4561 atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat aatgtttctc
     4621 atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca ttgaatacag
     4681 agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt taaagacagt
     4741 ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt accgatgact
     4801 gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc aacccctttt
     4861 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
     4921 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
     4981 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
     5041 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
     5101 gtgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
     5161 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
     5221 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
     5281 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
     5341 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
     5401 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
     5461 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
     5521 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
     5581 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
     5641 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
     5701 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
     5761 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
     5821 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     5881 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     5941 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     6001 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     6061 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     6121 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     6181 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     6241 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     6301 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     6361 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     6421 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     6481 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     6541 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     6601 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     6661 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     6721 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     6781 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     6841 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     6901 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     6961 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     7021 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     7081 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     7141 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     7201 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     7261 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     7321 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     7381 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     7441 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     7501 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     7561 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     7621 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     7681 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     7741 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     7801 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     7861 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     7921 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     7981 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     8041 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     8101 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     8161 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     8221 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     8281 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     8341 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     8401 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     8461 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     8521 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     8581 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     8641 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     8701 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     8761 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     8821 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     8881 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     8941 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     9001 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     9061 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     9121 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     9181 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     9241 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     9301 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     9361 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     9421 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     9481 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     9541 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     9601 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     9661 gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     9721 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     9781 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     9841 atttgcgcat tcgtaccagt tctttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     9901 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     9961 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
    10021 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
    10081 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
    10141 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
    10201 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
    10261 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
    10321 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
    10381 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
    10441 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
    10501 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
    10561 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
    10621 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
    10681 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
    10741 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
    10801 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
    10861 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
    10921 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
    10981 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
    11041 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
    11101 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
    11161 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
    11221 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
    11281 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
    11341 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
    11401 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
    11461 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
    11521 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
    11581 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
    11641 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
    11701 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
    11761 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
    11821 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
    11881 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
    11941 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
    12001 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
    12061 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
    12121 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
    12181 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
    12241 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
    12301 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
    12361 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
    12421 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
    12481 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
    12541 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
    12601 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
    12661 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
    12721 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
    12781 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
    12841 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
    12901 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
    12961 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
    13021 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
    13081 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
    13141 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
    13201 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
    13261 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
    13321 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
    13381 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
    13441 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
    13501 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
    13561 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
    13621 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
    13681 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
    13741 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
    13801 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
    13861 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
    13921 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
    13981 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
    14041 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
    14101 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
    14161 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
    14221 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
    14281 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
    14341 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
    14401 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
    14461 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
    14521 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
    14581 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
    14641 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
    14701 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
    14761 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
    14821 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    14881 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    14941 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    15001 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    15061 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    15121 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    15181 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    15241 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    15301 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    15361 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    15421 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    15481 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    15541 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    15601 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    15661 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    15721 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    15781 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    15841 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    15901 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    15961 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    16021 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    16081 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    16141 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    16201 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    16261 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    16321 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    16381 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    16441 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    16501 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    16561 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    16621 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    16681 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    16741 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    16801 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    16861 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    16921 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    16981 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    17041 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    17101 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    17161 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    17221 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    17281 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    17341 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    17401 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    17461 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    17521 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    17581 gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct
    17641 tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg
    17701 ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa
    17761 atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta
    17821 tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag
    17881 tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca
    17941 gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt
    18001 ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta
    18061 acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc
    18121 gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat
    18181 ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt
    18241 gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg
    18301 aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc
    18361 gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa
    18421 gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc
    18481 ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag
    18541 gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc
    18601 atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg
    18661 atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc
    18721 aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata
    18781 ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga
    18841 agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct
    18901 tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat
    18961 tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt
    19021 actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa
    19081 catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc
    19141 agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat
    19201 caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa
    19261 aattagagaa catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat
    19321 cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag
    19381 agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac
    19441 gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg
    19501 aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc
    19561 ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc
    19621 tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa
    19681 tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc
    19741 ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg
    19801 tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct
    19861 tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag
    19921 gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg
    19981 cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg
    20041 ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa
    20101 tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat
    20161 cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat
    20221 acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga
    20281 cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta
    20341 tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga
    20401 aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt
    20461 gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag
    20521 tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg
    20581 agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat
    20641 ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc
    20701 aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc
    20761 ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt
    20821 tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc
    20881 tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt
    20941 tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc
    21001 aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa
    21061 gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta
    21121 tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag
    21181 caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac
    21241 agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg
    21301 tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact
    21361 tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg
    21421 catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat
    21481 tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt
    21541 tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac
    21601 ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt
    21661 gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa
    21721 ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg
    21781 gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg
    21841 aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt
    21901 gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga
    21961 aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg
    22021 tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag
    22081 catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct
    22141 tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta
    22201 catattcagc atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag
    22261 aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca
    22321 aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg
    22381 tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt
    22441 atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt
    22501 gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag
    22561 ggccaaagat ggagctgcat ttatttgctt caaggcaaca tttcagagac aacacggtat
    22621 tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac
    22681 cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc
    22741 tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc
    22801 agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt
    22861 tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta
    22921 tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat
    22981 tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg
    23041 tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt
    23101 ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga
    23161 cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt
    23221 taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt
    23281 tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg
    23341 cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc
    23401 tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg
    23461 ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt
    23521 tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata
    23581 attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca
    23641 aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca
    23701 ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa
    23761 aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag
    23821 ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg
    23881 tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac
    23941 agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc
    24001 tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg
    24061 gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag
    24121 ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc
    24181 caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga
    24241 tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc
    24301 cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact
    24361 tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa
    24421 aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag
    24481 ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag
    24541 atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg
    24601 ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg
    24661 tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata
    24721 gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga
    24781 agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag
    24841 aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa
    24901 ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct
    24961 cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag
    25021 gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta
    25081 ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact
    25141 gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc
    25201 aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata
    25261 gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt
    25321 gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg
    25381 ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc
    25441 ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat
    25501 tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat
    25561 ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca
    25621 aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta
    25681 agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct
    25741 gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag
    25801 tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga
    25861 acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt
    25921 tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa
    25981 tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca
    26041 tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat
    26101 ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact
    26161 ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg
    26221 cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc
    26281 atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga
    26341 agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt
    26401 agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa
    26461 cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa
    26521 ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt
    26581 aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg
    26641 cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt
    26701 cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc
    26761 attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt
    26821 acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga
    26881 aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa
    26941 cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt
    27001 agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga
    27061 aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat
    27121 gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac
    27181 agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga
    27241 agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg
    27301 tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt
    27361 tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc
    27421 aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat
    27481 caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca
    27541 tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt
    27601 tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg
    27661 ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc
    27721 aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg
    27781 gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa
    27841 aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa
    27901 agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt
    27961 taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag
    28021 agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga
    28081 tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt
    28141 gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga
    28201 tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa
    28261 agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt
    28321 aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg
    28381 tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt
    28441 ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa
    28501 caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc
    28561 ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg
    28621 gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag
    28681 caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta
    28741 gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg
    28801 gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa
    28861 caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac
    28921 ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa
    28981 cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag
    29041 gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc
    29101 ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg
    29161 cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag
    29221 aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga
    29281 gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta
    29341 aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa
    29401 aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag
    29461 ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta
    29521 tcagatactg cttcatccat attgactttg atcggattta aaattgctgc aaagccagca
    29581 gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg
    29641 atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat
    29701 ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg
    29761 aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg
    29821 agagctacag caacagatag ttataatgat gtctttacta cactggctgt attgctttca
    29881 gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca
    29941 ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga
    30001 agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat
    30061 atacttggct atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca
    30121 gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg
    30181 atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg
    30241 cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa
    30301 acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa
    30361 tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta
    30421 cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat
    30481 tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag
    30541 tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat
    30601 ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga
    30661 ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa
    30721 ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta
    30781 gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa
    30841 gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca cctccactag
    30901 gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc
    30961 ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta
    31021 gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc
    31081 attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta
    31141 ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg
    31201 ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg
    31261 agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat
    31321 gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct
    31381 ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa
    31441 aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag
    31501 aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc
    31561 atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc
    31621 tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc
    31681 ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa
    31741 gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac
    31801 ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa
    31861 ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct
    31921 taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg
    31981 atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag
    32041 atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg
    32101 gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc
    32161 caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata
    32221 aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa
    32281 tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact
    32341 tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt
    32401 tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga
    32461 aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc
    32521 caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg
    32581 ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg
    32641 tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc
    32701 tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac
    32761 cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag
    32821 ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag
    32881 atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg
    32941 ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg
    33001 aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata
    33061 tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag
    33121 atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca
    33181 agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag
    33241 tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga
    33301 tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca
    33361 gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga
    33421 tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg
    33481 aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag
    33541 ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata
    33601 tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca
    33661 tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt
    33721 ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag cataatagga
    33781 gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat
    33841 tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct
    33901 tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta
    33961 tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat
    34021 ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt
    34081 cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata
    34141 tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa
    34201 tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg
    34261 tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac
    34321 agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg
    34381 cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc
    34441 ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt
    34501 tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata
    34561 aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa
    34621 gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg
    34681 gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga
    34741 tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg
    34801 gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat
    34861 tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc
    34921 aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt
    34981 ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg
    35041 tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa
    35101 aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg
    35161 attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat
    35221 tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt
    35281 atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa
    35341 cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt
    35401 acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc
    35461 cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac
    35521 cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag
    35581 atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc
    35641 atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg
    35701 ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg
    35761 ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc
    35821 gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc
    35881 caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg
    35941 ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac
    36001 tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag
    36061 ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg
    36121 cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc
    36181 ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta
    36241 gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta
    36301 gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg
    36361 ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct
    36421 gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa
    36481 aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta
    36541 tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct
    36601 acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag
    36661 gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac
    36721 ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg
    36781 ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac
    36841 caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg
    36901 cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc
    36961 aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga
    37021 aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc
    37081 ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca
    37141 accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga
    37201 ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc
    37261 agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt
    37321 gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag
    37381 atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg
    37441 gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg
    37501 tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac
    37561 atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat tagcggatac
    37621 atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt
    37681 gctgttccta actttgagat tccctttgta caatatgaac caaagctata tcttggagca
    37741 atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg
    37801 gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg
    37861 acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt
    37921 tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt
    37981 ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta
    38041 agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca
    38101 agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg
    38161 attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt
    38221 acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca
    38281 gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt
    38341 atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg
    38401 atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac
    38461 tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg
    38521 gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa
    38581 ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt
    38641 atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt
    38701 tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg
    38761 atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat
    38821 atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa
    38881 gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat
    38941 atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac
    39001 gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta
    39061 acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt
    39121 aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag
    39181 caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa
    39241 gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca
    39301 cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc
    39361 ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc
    39421 acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag
    39481 acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg
    39541 ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt
    39601 agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt
    39661 gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac
    39721 ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac
    39781 aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac
    39841 gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct
    39901 caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat
    39961 gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta
    40021 acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc
    40081 ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa
    40141 ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa
    40201 gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt
    40261 caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat
    40321 tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt
    40381 gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac
    40441 tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa
    40501 atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt
    40561 gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt
    40621 cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta tcactgatca
    40681 aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa
    40741 tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc
    40801 acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg
    40861 aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg
    40921 aacgatgaaa gctagtatcg ttgatgctgg agattccttc gaacatgcat ttgatcagct
    40981 gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc
    41041 aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt
    41101 acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga
    41161 gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa
    41221 agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg
    41281 tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa
    41341 attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt
    41401 tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat
    41461 cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc
    41521 cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt
    41581 atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac
    41641 gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg
    41701 cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag
    41761 tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag
    41821 tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag
    41881 atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag
    41941 catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg
    42001 atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc
    42061 ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct
    42121 atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa
    42181 atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat
    42241 gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt
    42301 cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata
    42361 caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc
    42421 ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc
    42481 aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt
    42541 ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta
    42601 aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg
    42661 cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata
    42721 agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg
    42781 ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta
    42841 cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag
    42901 tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca
    42961 ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc
    43021 atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa
    43081 cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag
    43141 aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca
    43201 cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa
    43261 atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta
    43321 tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg
    43381 caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta
    43441 tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc
    43501 tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg
    43561 aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg
    43621 cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag
    43681 gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag
    43741 ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc
    43801 gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag
    43861 tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag
    43921 tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa
    43981 tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca
    44041 cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc
    44101 atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct
    44161 taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga
    44221 aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta
    44281 aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa
    44341 tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg
    44401 cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg
    44461 acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc
    44521 tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag
    44581 tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta
    44641 aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt
    44701 tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag
    44761 cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc
    44821 gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga
    44881 aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa
    44941 aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg
    45001 tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac
    45061 ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc
    45121 cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca
    45181 tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt
    45241 atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca
    45301 tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga
    45361 cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga
    45421 acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt
    45481 ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc
    45541 ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt
    45601 ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta
    45661 gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc
    45721 ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc
    45781 aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca
    45841 tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa
    45901 cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct
    45961 tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca
    46021 atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat
    46081 tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat
    46141 gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga
    46201 ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca
    46261 agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc
    46321 gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc
    46381 ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct
    46441 atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct
    46501 gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag
    46561 gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta
    46621 ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa
    46681 tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg
    46741 gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa
    46801 tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc
    46861 gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc
    46921 cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc
    46981 aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag
    47041 caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag
    47101 gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc
    47161 agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag
    47221 gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat
    47281 ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag
    47341 cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt
    47401 actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat
    47461 tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag
    47521 caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc
    47581 gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc
    47641 agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg
    47701 aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt
    47761 tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt
    47821 attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc
    47881 tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc ttgatgaaga
    47941 tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact
    48001 gattgcgaga agcatctttt catcaggtat gaaaagaa
//
LOCUS       DAXWLB010000005        43722 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXWLB010000005 DAXWLB010000000
VERSION     DAXWLB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.005"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(24..602)
                     /locus_tag="ACGE4H_000209"
     CDS             complement(24..602)
                     /locus_tag="ACGE4H_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8363659.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(679..1209)
                     /locus_tag="ACGE4H_000210"
     CDS             complement(679..1209)
                     /locus_tag="ACGE4H_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFF8363660.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1213..2073)
                     /gene="rlmB"
                     /locus_tag="ACGE4H_000211"
     CDS             complement(1213..2073)
                     /gene="rlmB"
                     /locus_tag="ACGE4H_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFF8363661.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2066..2461)
                     /locus_tag="ACGE4H_000212"
     CDS             complement(2066..2461)
                     /locus_tag="ACGE4H_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFF8363662.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2458..3870)
                     /gene="cysS"
                     /locus_tag="ACGE4H_000213"
     CDS             complement(2458..3870)
                     /gene="cysS"
                     /locus_tag="ACGE4H_000213"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFF8363663.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3867..4403)
                     /gene="epsC"
                     /locus_tag="ACGE4H_000214"
     CDS             complement(3867..4403)
                     /gene="epsC"
                     /locus_tag="ACGE4H_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFF8363664.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4489..4728)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4725..6185)
                     /gene="gltX"
                     /locus_tag="ACGE4H_000215"
     CDS             complement(4725..6185)
                     /gene="gltX"
                     /locus_tag="ACGE4H_000215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFF8363665.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6355..7512)
                     /locus_tag="ACGE4H_000216"
     CDS             complement(6355..7512)
                     /locus_tag="ACGE4H_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFF8363666.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7546..8916)
                     /gene="radA"
                     /locus_tag="ACGE4H_000217"
     CDS             complement(7546..8916)
                     /gene="radA"
                     /locus_tag="ACGE4H_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFF8363667.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8979..9506)
                     /locus_tag="ACGE4H_000218"
     CDS             complement(8979..9506)
                     /locus_tag="ACGE4H_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFF8363668.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9736..10542
                     /gene="proB"
                     /locus_tag="ACGE4H_000219"
     CDS             9736..10542
                     /gene="proB"
                     /locus_tag="ACGE4H_000219"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFF8363669.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10539..11786
                     /locus_tag="ACGE4H_000220"
     CDS             10539..11786
                     /locus_tag="ACGE4H_000220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFF8363670.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11862..12206
                     /locus_tag="ACGE4H_000221"
     CDS             11862..12206
                     /locus_tag="ACGE4H_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363671.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12323..12649)
                     /locus_tag="ACGE4H_000222"
     CDS             complement(12323..12649)
                     /locus_tag="ACGE4H_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363672.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13022..14223)
                     /locus_tag="ACGE4H_000223"
                     /pseudo
     CDS             complement(13022..14223)
                     /locus_tag="ACGE4H_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14340..14855
                     /locus_tag="ACGE4H_000224"
     CDS             14340..14855
                     /locus_tag="ACGE4H_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8363673.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15054..15500)
                     /locus_tag="ACGE4H_000225"
     CDS             complement(15054..15500)
                     /locus_tag="ACGE4H_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFF8363674.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15547..17025)
                     /gene="msr(C)"
                     /locus_tag="ACGE4H_000226"
     CDS             complement(15547..17025)
                     /gene="msr(C)"
                     /locus_tag="ACGE4H_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8363675.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17379..18008)
                     /locus_tag="ACGE4H_000227"
     CDS             complement(17379..18008)
                     /locus_tag="ACGE4H_000227"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8363676.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(17992..18456)
                     /locus_tag="ACGE4H_000228"
     CDS             complement(17992..18456)
                     /locus_tag="ACGE4H_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8363677.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(18943..20211)
                     /locus_tag="ACGE4H_000229"
     CDS             complement(18943..20211)
                     /locus_tag="ACGE4H_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8363678.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20442..21179)
                     /locus_tag="ACGE4H_000230"
     CDS             complement(20442..21179)
                     /locus_tag="ACGE4H_000230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8363679.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21246..21815)
                     /locus_tag="ACGE4H_000231"
     CDS             complement(21246..21815)
                     /locus_tag="ACGE4H_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFF8363680.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            21943..22815
                     /locus_tag="ACGE4H_000232"
     CDS             21943..22815
                     /locus_tag="ACGE4H_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8363681.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23157..24029
                     /locus_tag="ACGE4H_000233"
     CDS             23157..24029
                     /locus_tag="ACGE4H_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8363682.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24026..25984
                     /gene="nagE"
                     /locus_tag="ACGE4H_000234"
     CDS             24026..25984
                     /gene="nagE"
                     /locus_tag="ACGE4H_000234"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFF8363683.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26235..27086)
                     /locus_tag="ACGE4H_000235"
     CDS             complement(26235..27086)
                     /locus_tag="ACGE4H_000235"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8363684.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27209..27676
                     /locus_tag="ACGE4H_000236"
     CDS             27209..27676
                     /locus_tag="ACGE4H_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFF8363685.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(27939..28421)
                     /locus_tag="ACGE4H_000237"
     CDS             complement(27939..28421)
                     /locus_tag="ACGE4H_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8363686.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28521..29384)
                     /locus_tag="ACGE4H_000238"
     CDS             complement(28521..29384)
                     /locus_tag="ACGE4H_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8363687.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            29982..30830
                     /locus_tag="ACGE4H_000239"
     CDS             29982..30830
                     /locus_tag="ACGE4H_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFF8363688.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(31001..31342)
                     /locus_tag="ACGE4H_000240"
     CDS             complement(31001..31342)
                     /locus_tag="ACGE4H_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFF8363689.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            31428..32135
                     /locus_tag="ACGE4H_000241"
     CDS             31428..32135
                     /locus_tag="ACGE4H_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8363690.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            32122..32445
                     /locus_tag="ACGE4H_000242"
     CDS             32122..32445
                     /locus_tag="ACGE4H_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8363691.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(32534..33571)
                     /locus_tag="ACGE4H_000243"
     CDS             complement(32534..33571)
                     /locus_tag="ACGE4H_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8363692.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(34018..35001)
                     /locus_tag="ACGE4H_000244"
     CDS             complement(34018..35001)
                     /locus_tag="ACGE4H_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFF8363693.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(35417..36187)
                     /locus_tag="ACGE4H_000245"
     CDS             complement(35417..36187)
                     /locus_tag="ACGE4H_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363694.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(36180..37115)
                     /locus_tag="ACGE4H_000246"
     CDS             complement(36180..37115)
                     /locus_tag="ACGE4H_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363695.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(37709..39358)
                     /locus_tag="ACGE4H_000247"
     CDS             complement(37709..39358)
                     /locus_tag="ACGE4H_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8363696.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(39553..40449)
                     /locus_tag="ACGE4H_000248"
     CDS             complement(39553..40449)
                     /locus_tag="ACGE4H_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8363697.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(40442..41122)
                     /locus_tag="ACGE4H_000249"
     CDS             complement(40442..41122)
                     /locus_tag="ACGE4H_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8363698.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(41123..41905)
                     /locus_tag="ACGE4H_000250"
     CDS             complement(41123..41905)
                     /locus_tag="ACGE4H_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8363699.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(41898..42815)
                     /locus_tag="ACGE4H_000251"
     CDS             complement(41898..42815)
                     /locus_tag="ACGE4H_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8363700.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(42818..43102)
                     /locus_tag="ACGE4H_000252"
     CDS             complement(42818..43102)
                     /locus_tag="ACGE4H_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363701.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT        12615 a         9302 c         7839 g        13966 t
ORIGIN      
        1 aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt
       61 ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca
      121 gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt
      181 gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa
      241 ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg
      301 attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa
      361 tagatttgat tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt
      421 tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa
      481 acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga
      541 tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac
      601 ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga
      661 actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa
      721 tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc
      781 tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc
      841 agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc
      901 cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc
      961 agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg
     1021 gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt catatttcgc
     1081 ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt
     1141 aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg
     1201 ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc
     1261 agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc
     1321 catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat
     1381 tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca
     1441 aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg
     1501 gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg
     1561 gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc
     1621 aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc
     1681 ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac
     1741 gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt
     1801 gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga
     1861 atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg
     1921 gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt
     1981 tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt
     2041 cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc
     2101 agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg
     2161 cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc
     2221 ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt
     2281 ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac
     2341 gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt
     2401 cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca
     2461 tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt
     2521 caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg
     2581 ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc
     2641 gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac
     2701 tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc
     2761 atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa
     2821 caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt
     2881 caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt
     2941 ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg
     3001 tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat
     3061 tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt
     3121 tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc
     3181 ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca
     3241 ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg
     3301 ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc
     3361 tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta atttcccata
     3421 attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt
     3481 ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg
     3541 cagtgtagca ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg
     3601 atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc
     3661 tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg
     3721 aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt
     3781 cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt
     3841 tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt
     3901 cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca
     3961 acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca
     4021 ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct
     4081 gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg
     4141 ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga
     4201 tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag
     4261 agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg
     4321 tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca
     4381 atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa
     4441 atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct
     4501 ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt
     4561 atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc
     4621 aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc
     4681 tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga
     4741 tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga
     4801 ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg
     4861 ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc
     4921 atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt
     4981 acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca
     5041 acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat
     5101 tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga
     5161 cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc
     5221 agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg
     5281 atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca
     5341 ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc
     5401 aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc
     5461 atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt
     5521 gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg
     5581 accgcaacag caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca
     5641 ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct
     5701 gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct
     5761 tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg
     5821 cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga
     5881 ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg
     5941 taagggccgt attctccagg attttcagga gattcatccc aattcattcc caaccaagca
     6001 aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca
     6061 atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt
     6121 cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc
     6181 gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt
     6241 acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg
     6301 taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt
     6361 tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc
     6421 aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc
     6481 gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag
     6541 gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc
     6601 accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac
     6661 attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa
     6721 aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc
     6781 gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg
     6841 acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag
     6901 aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat
     6961 ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga
     7021 ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct
     7081 tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag
     7141 atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat
     7201 attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc
     7261 accgaagaga agataagtca agctgacctc acttagtttt tgttctactt gtttgataat
     7321 agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag
     7381 gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc
     7441 tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac
     7501 acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg
     7561 cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca
     7621 gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt
     7681 gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat
     7741 gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat
     7801 agctaaatca atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc
     7861 ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg
     7921 attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa
     7981 tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc
     8041 agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa
     8101 tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac
     8161 tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac
     8221 catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat
     8281 aaaaatggct atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac
     8341 ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc
     8401 aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt
     8461 ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat
     8521 ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg
     8581 ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac
     8641 catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt
     8701 gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc
     8761 tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt
     8821 attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt
     8881 ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat
     8941 catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat
     9001 ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc
     9061 ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat
     9121 tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct
     9181 actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc
     9241 aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat
     9301 tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt
     9361 ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag
     9421 ccagcagcat gatgtgtagc gcgctgcggt agattgatgt tttgatcttt atatttagaa
     9481 ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct
     9541 aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt
     9601 aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt
     9661 ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt
     9721 tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta
     9781 ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta
     9841 gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga
     9901 gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa
     9961 cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc
    10021 agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct
    10081 gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct
    10141 atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc
    10201 gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc
    10261 ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg
    10321 tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc
    10381 acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca
    10441 aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca
    10501 ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag
    10561 gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg
    10621 agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa
    10681 atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct
    10741 tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt
    10801 tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg
    10861 gcaaaaagcg cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca
    10921 cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca
    10981 aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg
    11041 ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac
    11101 agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa
    11161 tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc
    11221 acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa
    11281 aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg
    11341 cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg
    11401 cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg
    11461 atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa
    11521 ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg
    11581 cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca
    11641 ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa
    11701 agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt
    11761 atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc
    11821 ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga
    11881 ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat
    11941 cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt
    12001 attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg
    12061 cattatgctt attagcgaca atcaattact attctttttt cgatcattca atcgcttata
    12121 cgattacgct actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa
    12181 accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag
    12241 atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt
    12301 tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa
    12361 gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca
    12421 agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca
    12481 cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga
    12541 ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg
    12601 gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc
    12661 ccattctttt tcttaaatga attttagctt acctactgaa aattttccag tttcattatt
    12721 ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg
    12781 tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt
    12841 ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac
    12901 ggcgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct
    12961 atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt
    13021 cttattcttc ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga
    13081 taagaacaat ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca
    13141 cacctgcaat aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg
    13201 aagcaacgtt tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga
    13261 taattccttg gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag
    13321 ttccagctcc taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc
    13381 gataagtcgt aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa
    13441 gcacacccca gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa
    13501 atggcgcata cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa
    13561 ctgaacgaag tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct
    13621 ttggtttctc agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa
    13681 atgctacccc aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag
    13741 ggccaacagc cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt
    13801 taccaactaa aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga
    13861 caccccagcc ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac
    13921 cagcgaatac aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact
    13981 agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa
    14041 cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc
    14101 tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact
    14161 tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc
    14221 catccctttc tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt
    14281 aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt aggattttta
    14341 tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg
    14401 aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc
    14461 ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa
    14521 aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg
    14581 aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc
    14641 tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca
    14701 tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac
    14761 gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc
    14821 aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa
    14881 tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc
    14941 gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca
    15001 agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca
    15061 ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac
    15121 ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca
    15181 ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc
    15241 atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta
    15301 aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg
    15361 aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa
    15421 ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct
    15481 tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta
    15541 gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac
    15601 ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg
    15661 atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg
    15721 ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt
    15781 cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa
    15841 gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg
    15901 aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac
    15961 tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt
    16021 acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc
    16081 atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata
    16141 gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg
    16201 cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat
    16261 ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg
    16321 ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat
    16381 cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt
    16441 tttctccaat tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc
    16501 tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact
    16561 aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt
    16621 tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag
    16681 acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc
    16741 cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata
    16801 ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa
    16861 aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat
    16921 ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc
    16981 aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct
    17041 atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa
    17101 aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct
    17161 gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa
    17221 ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa
    17281 tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca
    17341 aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt
    17401 tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata
    17461 ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg
    17521 aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata
    17581 gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg
    17641 taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat
    17701 tcgtaagctt ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc
    17761 atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag
    17821 atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa
    17881 gaaggtgcag tcgtcgcttc ttttatcatt tccagcattt cttcgtgtgg gattttgtat
    17941 gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt
    18001 tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa
    18061 tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac
    18121 ggaacctgta tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt
    18181 tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt
    18241 tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg
    18301 caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt
    18361 aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat
    18421 ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga
    18481 ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc
    18541 tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa
    18601 tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca
    18661 aactatagaa gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat
    18721 cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat
    18781 aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt
    18841 gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg
    18901 ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc
    18961 tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt
    19021 atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc
    19081 accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc
    19141 agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg
    19201 ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag
    19261 ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag
    19321 acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc
    19381 gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca
    19441 agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac
    19501 gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta
    19561 cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga
    19621 agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt
    19681 aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat
    19741 gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc
    19801 aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt
    19861 ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg
    19921 gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc
    19981 ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga
    20041 gacagtagct gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact
    20101 cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac
    20161 tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct
    20221 cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca
    20281 atgtccatat ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa
    20341 gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac
    20401 tatttaacaa gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc
    20461 ggacggataa tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca
    20521 gcagaagcta tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga
    20581 gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg
    20641 atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg
    20701 acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca
    20761 gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa
    20821 attccattta acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga
    20881 ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga
    20941 ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct
    21001 gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct
    21061 tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct
    21121 tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt
    21181 ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt
    21241 actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg
    21301 gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca
    21361 atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg
    21421 attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg
    21481 aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg
    21541 aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct
    21601 gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct
    21661 agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct
    21721 tcgttttcag cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct
    21781 ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact
    21841 ttataacgga cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa
    21901 ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg
    21961 acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat
    22021 atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca
    22081 cttttcactc ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca
    22141 aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca
    22201 atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct
    22261 gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg
    22321 aatgcagatg atatcataga caaactggat agcggattac ttgattttgg actcgtaatc
    22381 gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga
    22441 cttttgatac atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg
    22501 aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg
    22561 ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct
    22621 tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact
    22681 tatgggacgg aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc
    22741 atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg
    22801 gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag
    22861 ataagcaaaa gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat
    22921 ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt
    22981 ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca
    23041 tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg
    23101 ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga
    23161 aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag
    23221 tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg
    23281 atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta
    23341 atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg
    23401 ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt
    23461 ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag
    23521 tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc
    23581 ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac
    23641 gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca
    23701 tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt
    23761 attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg
    23821 ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga
    23881 tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag
    23941 aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg
    24001 ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct
    24061 tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg
    24121 ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt
    24181 aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc
    24241 aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac
    24301 tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca
    24361 gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg
    24421 tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc
    24481 aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc
    24541 ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc
    24601 aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa
    24661 ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga
    24721 aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg
    24781 gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac
    24841 agcttccttg atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact
    24901 tgaattttca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg
    24961 gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg
    25021 atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat
    25081 cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa
    25141 attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac
    25201 gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg
    25261 agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga
    25321 tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat
    25381 cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat
    25441 ggcaaaacta catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac
    25501 agaaacattt gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact
    25561 gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta
    25621 tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat
    25681 tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca
    25741 tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg
    25801 tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc
    25861 aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga
    25921 aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc
    25981 ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa
    26041 tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat
    26101 aagtccagct aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg
    26161 atgcagaaca gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac
    26221 aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt
    26281 aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta
    26341 atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt
    26401 agattttcga caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca
    26461 cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt
    26521 tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat
    26581 gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc
    26641 ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata
    26701 cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag
    26761 tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc
    26821 ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat
    26881 cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt
    26941 atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag
    27001 atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca
    27061 gttgctcctg ttactaaata atgcatattt gccactccct attcttttta tttgttaaga
    27121 ttcacagctg tacatttata aattacacct ttatcataaa gagtgtggta agataagtca
    27181 agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat
    27241 ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat
    27301 tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa
    27361 atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga
    27421 tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt
    27481 cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga
    27541 aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag
    27601 tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt
    27661 agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca
    27721 taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg
    27781 ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct
    27841 cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat
    27901 gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt
    27961 atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga
    28021 cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc
    28081 aaatcatttc ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct
    28141 tttgggaatg ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca
    28201 tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca
    28261 gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc
    28321 tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc
    28381 accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt
    28441 atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta
    28501 gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa
    28561 acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca
    28621 taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg
    28681 ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct
    28741 aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc
    28801 ttttcttcca agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat
    28861 aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt
    28921 tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca
    28981 taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata
    29041 aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga
    29101 tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta
    29161 aacgccgttt ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta
    29221 cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat
    29281 tgatattcag agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa
    29341 tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt
    29401 gattgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt
    29461 tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa
    29521 cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga
    29581 agacagattc taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct
    29641 tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat
    29701 ttttctttgc gatacatcat acctctttta tcaattggaa caattattta tttctaacaa
    29761 aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc
    29821 tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa
    29881 ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt
    29941 tatactaagt ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt
    30001 attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc
    30061 atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg
    30121 tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag
    30181 gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa
    30241 aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg
    30301 tacgggagct tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag
    30361 ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc atccataaat
    30421 acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc
    30481 gaaagttcat ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat
    30541 tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg
    30601 aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag
    30661 cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg
    30721 acgcctctta ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag
    30781 aattttctaa ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga
    30841 tttcaatgaa aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc
    30901 agaccttgct ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta
    30961 cccaatatta gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt
    31021 ccttcgttgc tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat
    31081 tttgtaagag aggttccgct gcatctgtat attggataaa attctgacaa caactttcag
    31141 gtgtgtcgaa ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt
    31201 cctccatact taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg
    31261 caagattttt cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt
    31321 ctttacgttt caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt
    31381 ttaacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt
    31441 tagatataaa aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg
    31501 gacttgcatt tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga
    31561 tgtccctgct ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc
    31621 acagcccatt gctttccatc gttttctcga cctttctagt caattcccga atgattctga
    31681 tgagtatgac gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg
    31741 gcacattgct gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac
    31801 aaaagttaaa ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg
    31861 gttcttctct tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt
    31921 tggatttcgc catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata
    31981 gaaacacctc taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg
    32041 tcggactgat ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg
    32101 gattgggggt gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt
    32161 tgtactttag caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat
    32221 ttaccgcaac cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta
    32281 tggttcagca gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat
    32341 gctttggctt cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc
    32401 gtcttggcag gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag
    32461 cttatggcaa attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc
    32521 ggcgtattct tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc
    32581 ctgtttctac gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga
    32641 ccttgctcaa tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag
    32701 cttctccttg cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt
    32761 cgaagctgat agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga
    32821 tcgttcccac atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa
    32881 tatcgaataa tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac
    32941 ttaagtcgtg atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat
    33001 tcttcggact tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa
    33061 tcgaaccgac gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt
    33121 cttctggagt tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg
    33181 gcaaggctgc agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt
    33241 caactagttg gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc
    33301 gatcgccgac agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat
    33361 caaaacctaa gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga
    33421 caggattaac agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta
    33481 tctgttcttt ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac
    33541 cgactacctt cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg
    33601 gagcatccat acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta
    33661 ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga
    33721 ggttgcgaca agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg
    33781 gtcacaagtc aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga
    33841 ctcagagtta aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc
    33901 tcggtattag ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat
    33961 tacttgatac aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca
    34021 ttgttcactg aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc
    34081 atcagtagat ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt
    34141 gatatagatt ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata
    34201 atgatcttgc agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc
    34261 cctcgggtct ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc
    34321 atcaacgcca tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt
    34381 gatcgtgatt agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac
    34441 ttcccaatgt gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt
    34501 ggctatatct tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata
    34561 gccgatacgc gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg
    34621 tgccgctact tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg
    34681 gactgggcag cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct
    34741 catttctcta aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc
    34801 ttctgtaaat gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc
    34861 tgagtttgta aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg
    34921 aaatacaaca tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc
    34981 tttccaaaaa ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc
    35041 aatctttctc aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag
    35101 cttaaaagat gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc
    35161 attttccgga ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg
    35221 aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt
    35281 tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac
    35341 aacaaaatat gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga
    35401 tttcacaacc tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa
    35461 cggcgtacaa aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat
    35521 cgatcgttcc aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt
    35581 ttgaaacagc ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat
    35641 aagttaacgg ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa
    35701 aaatcccgtt gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga
    35761 tgaccatgaa ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga
    35821 gagagccgac gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat
    35881 gcgtaggaac aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga
    35941 aggacgaaga aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa
    36001 cgatgaattc catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc
    36061 cgaaaataac aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata
    36121 tacgcaaata gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat
    36181 taaacatttt tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct
    36241 aaacgattcg acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg
    36301 aagattccgt tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca
    36361 aatgtacaag catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata
    36421 aatgtttcaa attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca
    36481 cccgattgga tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc
    36541 gtcaaaatga tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt
    36601 ctcaactcga tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc
    36661 atcaatgccc tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg
    36721 acatttctct tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct
    36781 tctttgcgag agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca
    36841 aaagggttaa aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga
    36901 tccttgtcca gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa
    36961 gtaataatcc cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa
    37021 tctccttctt tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc
    37081 tttttcaaat tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat
    37141 gattcttaat tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa
    37201 acacacctgc taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata
    37261 actatctaaa caactctgga acttttaaaa tcataaaagt accgacagca actactacga
    37321 tgacatatag aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc
    37381 ctccgtcctt ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa
    37441 tagttatttt caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt
    37501 gcgtctcgtc gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg
    37561 tttattttgt atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc
    37621 cattgaattc tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga
    37681 cccacgcatc atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat
    37741 cgaacaacaa actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt
    37801 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    37861 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    37921 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    37981 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa
    38041 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga
    38101 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    38161 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    38221 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    38281 tggaacccgt gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta
    38341 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    38401 tgatcggcat ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca
    38461 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    38521 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac
    38581 tcccgctctt cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    38641 gctgtttcag ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa
    38701 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    38761 gataatagac cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc
    38821 ctaaaagaag caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    38881 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    38941 tttttcctaa acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat
    39001 aaaaagcaat tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct
    39061 gatacaacgc ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga
    39121 acatatccaa gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt
    39181 tctccgtgag ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa
    39241 tttttcccca attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa
    39301 caactaataa aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat
    39361 ccctctccgt tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg
    39421 ggtacaaaaa taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac
    39481 cttccaaaaa agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta
    39541 aattagagcc aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa
    39601 tctagcggtc atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat
    39661 tccgctagat tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt
    39721 ttcaacagag gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga
    39781 atcctctttt gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat
    39841 gatattttgc agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga
    39901 gtgttcagcg aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa
    39961 tacttcttcc acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt
    40021 ctctattcga gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg
    40081 tttgatgatc gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg
    40141 tacataaccg ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga
    40201 tagtctgatg ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac
    40261 ccatgtattc agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag
    40321 acgaccacca tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat
    40381 ttccctttgc aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca
    40441 cttaatcaaa tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg
    40501 ctagtttccc tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg
    40561 gttcttgcca aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta
    40621 aaaaaagctt cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat
    40681 ttacatccct cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat
    40741 cttttacttg gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt
    40801 cgatatcaaa tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat
    40861 ctctttgatc gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt
    40921 ctgctaacaa tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga
    40981 caggatatgt gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt
    41041 atctctgttc aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca
    41101 aaatagtttc cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta
    41161 gagctgatga taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat
    41221 aagttgcttg ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta
    41281 gaaattccaa gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat
    41341 aaagaaaaga aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg
    41401 gaacagataa tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat
    41461 ttccatagtt tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca
    41521 tggctagccc cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt
    41581 tcgacacata ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt
    41641 tttgaaattc ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg
    41701 cagccatttt ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt
    41761 ggtttaattc attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg
    41821 gaagtacgac catcagtcct agtgagaccc acattttacg gctcttccat acttttattt
    41881 tttcagcagt taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct
    41941 tccagttttt gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct
    42001 tgattgatgg ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg
    42061 acaaaataat ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa
    42121 gtgtcaattt ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg
    42181 atcaagtgtc cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc
    42241 aagtggctag aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct
    42301 cgcatttctg ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat
    42361 tctggattcg tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa
    42421 ttctttgctt tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt
    42481 tctctatccg acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat
    42541 tctggataaa gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa
    42601 tccgtcgttc caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt
    42661 ctcataaagg tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt
    42721 ttgatttcga tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca
    42781 gttgctttca agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa
    42841 gtgaagtata gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat
    42901 tttccaattg aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc
    42961 aatcaaagag cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg
    43021 ttatcgttga catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca
    43081 tataaaaagc aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc
    43141 cgtttgggat ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc
    43201 ccttcttccg aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta
    43261 tcaaacatga gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg
    43321 tcacaacctc agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt
    43381 gtttatagat cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca
    43441 caaaaaaggc actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc
    43501 gctcagcatt gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca
    43561 attaacgttt tgagaactgt gaagctttac gggctttctt aagacctggt tttttcttta
    43621 tatcattgaa tagccttgaa aaaccttgat ataaaaaggt tttagtaaaa atgattttat
    43681 caaatttgaa taactttgaa atatttgtgt tctaaaatgt gt
//
LOCUS       DAXWLB010000006        36251 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXWLB010000006 DAXWLB010000000
VERSION     DAXWLB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.006"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(88..996)
                     /locus_tag="ACGE4H_000253"
     CDS             complement(88..996)
                     /locus_tag="ACGE4H_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8363702.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            1179..2303
                     /locus_tag="ACGE4H_000254"
     CDS             1179..2303
                     /locus_tag="ACGE4H_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8363703.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            2530..3447
                     /locus_tag="ACGE4H_000255"
     CDS             2530..3447
                     /locus_tag="ACGE4H_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8363704.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            3440..4273
                     /locus_tag="ACGE4H_000256"
     CDS             3440..4273
                     /locus_tag="ACGE4H_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8363705.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            4356..5474
                     /locus_tag="ACGE4H_000257"
     CDS             4356..5474
                     /locus_tag="ACGE4H_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8363706.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            5495..7366
                     /locus_tag="ACGE4H_000258"
     CDS             5495..7366
                     /locus_tag="ACGE4H_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFF8363707.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            7360..8583
                     /locus_tag="ACGE4H_000259"
     CDS             7360..8583
                     /locus_tag="ACGE4H_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8363708.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(8669..10603)
                     /locus_tag="ACGE4H_000260"
     CDS             complement(8669..10603)
                     /locus_tag="ACGE4H_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8363709.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            10829..11485
                     /locus_tag="ACGE4H_000261"
     CDS             10829..11485
                     /locus_tag="ACGE4H_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8363710.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(11559..11912)
                     /locus_tag="ACGE4H_000262"
     CDS             complement(11559..11912)
                     /locus_tag="ACGE4H_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8363711.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            12104..13033
                     /locus_tag="ACGE4H_000263"
     CDS             12104..13033
                     /locus_tag="ACGE4H_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFF8363712.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            13044..13880
                     /locus_tag="ACGE4H_000264"
     CDS             13044..13880
                     /locus_tag="ACGE4H_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFF8363713.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            14128..14904
                     /locus_tag="ACGE4H_000265"
     CDS             14128..14904
                     /locus_tag="ACGE4H_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8363714.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            15080..15824
                     /locus_tag="ACGE4H_000266"
                     /pseudo
     CDS             15080..15824
                     /locus_tag="ACGE4H_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            15817..17397
                     /locus_tag="ACGE4H_000267"
     CDS             15817..17397
                     /locus_tag="ACGE4H_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFF8363715.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(17648..18127)
                     /locus_tag="ACGE4H_000268"
     CDS             complement(17648..18127)
                     /locus_tag="ACGE4H_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8363716.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18143..18895)
                     /locus_tag="ACGE4H_000269"
     CDS             complement(18143..18895)
                     /locus_tag="ACGE4H_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFF8363717.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            19012..19082
                     /locus_tag="ACGE4H_000270"
     tRNA            19012..19082
                     /locus_tag="ACGE4H_000270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19044..19046,aa:Gly,seq:ccc)
     gene            19443..20168
                     /locus_tag="ACGE4H_000271"
     CDS             19443..20168
                     /locus_tag="ACGE4H_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8363718.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            20423..20689
                     /locus_tag="ACGE4H_000272"
     CDS             20423..20689
                     /locus_tag="ACGE4H_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFF8363719.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            20686..21117
                     /gene="ruvX"
                     /locus_tag="ACGE4H_000273"
     CDS             20686..21117
                     /gene="ruvX"
                     /locus_tag="ACGE4H_000273"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFF8363720.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            21142..21447
                     /locus_tag="ACGE4H_000274"
     CDS             21142..21447
                     /locus_tag="ACGE4H_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFF8363721.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21528..22673)
                     /locus_tag="ACGE4H_000275"
     CDS             complement(21528..22673)
                     /locus_tag="ACGE4H_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8363722.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22734..23381)
                     /locus_tag="ACGE4H_000276"
     CDS             complement(22734..23381)
                     /locus_tag="ACGE4H_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8363723.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            23575..23868
                     /locus_tag="ACGE4H_000277"
     CDS             23575..23868
                     /locus_tag="ACGE4H_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363724.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            23912..24223
                     /locus_tag="ACGE4H_000278"
     CDS             23912..24223
                     /locus_tag="ACGE4H_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363725.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            24220..24844
                     /locus_tag="ACGE4H_000279"
                     /pseudo
     CDS             24220..24844
                     /locus_tag="ACGE4H_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24985..25143
                     /locus_tag="ACGE4H_000280"
     CDS             24985..25143
                     /locus_tag="ACGE4H_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363726.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            25144..25506
                     /locus_tag="ACGE4H_000281"
     CDS             25144..25506
                     /locus_tag="ACGE4H_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363727.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            25543..26391
                     /locus_tag="ACGE4H_000282"
     CDS             25543..26391
                     /locus_tag="ACGE4H_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFF8363728.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            26381..27856
                     /locus_tag="ACGE4H_000283"
     CDS             26381..27856
                     /locus_tag="ACGE4H_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFF8363729.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            28122..28529
                     /locus_tag="ACGE4H_000284"
     CDS             28122..28529
                     /locus_tag="ACGE4H_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFF8363730.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            28532..28747
                     /locus_tag="ACGE4H_000285"
     CDS             28532..28747
                     /locus_tag="ACGE4H_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363731.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            28751..29131
                     /locus_tag="ACGE4H_000286"
     CDS             28751..29131
                     /locus_tag="ACGE4H_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8363732.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            29265..29420
                     /locus_tag="ACGE4H_000287"
     CDS             29265..29420
                     /locus_tag="ACGE4H_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFF8363733.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            29489..29962
                     /locus_tag="ACGE4H_000288"
     CDS             29489..29962
                     /locus_tag="ACGE4H_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFF8363734.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            29959..31653
                     /locus_tag="ACGE4H_000289"
     CDS             29959..31653
                     /locus_tag="ACGE4H_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8363735.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            31619..31804
                     /locus_tag="ACGE4H_000290"
     CDS             31619..31804
                     /locus_tag="ACGE4H_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363736.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            31808..32983
                     /locus_tag="ACGE4H_000291"
     CDS             31808..32983
                     /locus_tag="ACGE4H_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8363737.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            32976..34499
                     /locus_tag="ACGE4H_000292"
     CDS             32976..34499
                     /locus_tag="ACGE4H_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8363738.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            34555..34839
                     /locus_tag="ACGE4H_000293"
     CDS             34555..34839
                     /locus_tag="ACGE4H_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFF8363739.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            34826..35161
                     /locus_tag="ACGE4H_000294"
     CDS             34826..35161
                     /locus_tag="ACGE4H_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8363740.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            35456..36082
                     /locus_tag="ACGE4H_000295"
     CDS             35456..36082
                     /locus_tag="ACGE4H_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363741.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
BASE COUNT        12154 a         5861 c         7282 g        10954 t
ORIGIN      
        1 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
       61 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
      121 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
      181 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
      241 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
      301 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
      361 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
      421 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
      481 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
      541 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
      601 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
      661 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
      721 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
      781 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
      841 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
      901 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
      961 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     1021 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     1081 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     1141 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     1201 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     1261 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     1321 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     1381 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     1441 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     1501 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     1561 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     1621 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     1681 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     1741 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     1801 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     1861 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     1921 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     1981 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     2041 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     2101 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcca
     2161 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     2221 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     2281 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     2341 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     2401 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     2461 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     2521 gacttaacga tgatacaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     2581 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     2641 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     2701 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     2761 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     2821 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     2881 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     2941 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     3001 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     3061 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     3121 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     3181 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     3241 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     3301 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     3361 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     3421 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     3481 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     3541 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     3601 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     3661 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     3721 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     3781 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     3841 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     3901 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     3961 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     4021 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     4081 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     4141 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     4201 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     4261 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     4321 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     4381 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     4441 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     4501 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     4561 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     4621 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     4681 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     4741 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     4801 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     4861 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     4921 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     4981 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     5041 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     5101 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     5161 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     5221 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     5281 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     5341 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     5401 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     5461 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     5521 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     5581 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     5641 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     5701 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     5761 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     5821 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     5881 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     5941 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     6001 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     6061 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     6121 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     6181 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     6241 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     6301 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     6361 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     6421 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     6481 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     6541 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     6601 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     6661 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     6721 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     6781 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     6841 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     6901 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     6961 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     7021 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     7081 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     7141 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     7201 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     7261 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     7321 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     7381 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     7441 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     7501 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     7561 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     7621 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     7681 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     7741 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
     7801 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
     7861 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
     7921 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
     7981 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
     8041 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
     8101 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
     8161 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
     8221 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
     8281 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
     8341 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
     8401 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
     8461 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
     8521 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
     8581 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
     8641 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
     8701 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
     8761 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
     8821 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
     8881 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
     8941 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
     9001 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
     9061 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
     9121 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
     9181 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
     9241 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
     9301 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
     9361 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
     9421 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
     9481 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
     9541 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
     9601 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
     9661 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
     9721 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
     9781 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
     9841 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
     9901 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
     9961 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    10021 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    10081 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    10141 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    10201 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    10261 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    10321 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    10381 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    10441 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    10501 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    10561 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    10621 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    10681 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    10741 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    10801 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    10861 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    10921 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    10981 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    11041 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    11101 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    11161 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    11221 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    11281 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    11341 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    11401 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    11461 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    11521 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    11581 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    11641 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    11701 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    11761 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    11821 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    11881 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    11941 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    12001 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    12061 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    12121 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    12181 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    12241 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    12301 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    12361 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    12421 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    12481 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    12541 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    12601 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    12661 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    12721 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    12781 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    12841 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    12901 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    12961 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    13021 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    13081 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    13141 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    13201 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    13261 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    13321 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    13381 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    13441 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    13501 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    13561 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    13621 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    13681 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    13741 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    13801 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    13861 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    13921 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    13981 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    14041 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    14101 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    14161 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    14221 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    14281 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    14341 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    14401 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    14461 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    14521 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    14581 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    14641 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    14701 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    14761 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    14821 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    14881 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    14941 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    15001 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    15061 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    15121 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    15181 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    15241 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    15301 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    15361 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaaagaa aagcgtttag
    15421 cagaattgac agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt
    15481 cacatggaat gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt
    15541 gggtattgga tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa
    15601 tgatgaaggc tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata
    15661 ccgcacaaca gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg
    15721 gggcagttag tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga
    15781 aaatggctgg aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa
    15841 ttaattcgag ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa
    15901 ggaaaaaagg gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg
    15961 atttttttac tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt
    16021 ttctttactt attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc
    16081 atttataata tcttttttga aggaaatgac ctgcaagtat ttttgcctct gccttttgga
    16141 caagaacagg ttttttcagc aaagattttg gtagttgctt tgaccatcat tccttttgtt
    16201 tttccgcttc tagtcatgtt tattttaacc ggatggcgag ctgatctagg gattttgctt
    16261 actatcatta tctcaatcct tttgttttct ctatttttga ttattctttt ttgtctggct
    16321 agtttgattg tttttggttt ggctcgaaca gcagtgttta aaaaacatag aaaaacagtt
    16381 tctagtatct tgcttggcag ctcgatgctg atcgcatttg ctggaatcat gtggatgaat
    16441 catcaaacgg ggactattga aaatgagtta acagatcggc atccgatttc gatattattg
    16501 ccattctatg atgctgccag tcagccactt tctcaacaag gtttattggg ttggggaata
    16561 ctagctatca cggctctggg atttttcctt gctgtaaaac agctgatagt accaaagctt
    16621 acagagcaac tgactgattc aaacagtgag ttcaaaataa aaagaaccca taaaaagaac
    16681 caaaatgtgc atcagctttt atttagttac aatgctcaat tagtaaaaga acctaatttg
    16741 attatgcaag ttctttcttc ttccttgtta actccaatca tttttattct tgcttttgcc
    16801 ctaggaggcg aaataaaatt aactgattta gataataagt taatcggtgt tgttttcctg
    16861 gtagggatcg cattggcttt cttaacagtt aatcagactt cttttatcag caatcttatt
    16921 tctttggatc aagaaaattt tctgtttatc cgttctttgc caatctctat gaaccaatat
    16981 ttaaaagaaa agttcagatt cggatggata ttgcagagtt ttttaactgg tgggatcgca
    17041 ttattaagcg gactgtcttt tcagctgcct ttgtttttta tccctagctt gctatttggt
    17101 tctttattcg gctgctactt attgtcacta cgttatttcg ctagagatta tcggttgctg
    17161 ctgctgaact ggaccaatat taaccaatta tttaacagag gttcaggaag tttgggttta
    17221 gtcgcaacta tgatgggttc attgatcatc agtattattc ttatcgtctt atacggaatg
    17281 ttggctttgt tttatccatt ttggttagtg aatattcctg tgatcggttt agttctaata
    17341 ctttccgctc tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt tgaataaaga
    17401 agaggggccg tgacaaaaat catgtcgcag cccctttttt acagatagag ggtgttcaaa
    17461 agtcagtttc tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta tttttacaaa
    17521 acttttttta atactcataa ttacttctta ttgctcctgc ggttacttgt cgcaatgttt
    17581 gtgagatatt ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt tctttgttac
    17641 acttcgttta tttaaaaagc cgtttttttc gtttcatcat gattgcgtca cgctttactt
    17701 tgaaattacg gatgttatgg ttaaagactt ctgataatac aagacctaaa gcgattgctc
    17761 cagcaatcat aattgcttgg accgcataat gagccgcttc tcgataggat tcagtaacga
    17821 gatttcgaac cgcttgataa gctaaaccac cagggactag aggaaccatc ccgggaatat
    17881 tgaaaatcgt tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg aatgctactc
    17941 ctaaagaacc aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca cagatccagt
    18001 agatcatcca gccaaaagca ccagaaagac cacaacaaat caatgaacgc ctaggtacat
    18061 ttgtaataac ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga ataaatatcg
    18121 tccccatact tgtgttcctt tcctaataaa aaagctggaa aataaaagca atcgcaaaac
    18181 cgatcatcgt agcagtcatc atcgcttctg ttccgcgaga aactcctgag acataatgtc
    18241 cagctagcaa atcacggata gcgttcgtaa tttgaactcc aggaactaaa ggcatcacac
    18301 atccgataat gattagatcc tgattgcttc ctaagccaaa acgtacgctg tagatagcgg
    18361 ctatgccaat caataaagaa gataaaaatt ccgataaaaa tttgattcta aaaaatttca
    18421 aactatatag ataagctgcg tatcccaatc ctccaatgac taaagtcaca ggtaaatcgg
    18481 tcatctttcc gccaaataac accataattg ttccactgat cactgccgcg ctaacgattt
    18541 gcaaccagac aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg cagtaaagtt
    18601 cttctaatgt tagctctgaa gcaacatatt tacgtgaaag atcatttact tttactactt
    18661 tttctaaatt aatcgaacgg ttggtaattt gttccattcg gatggtcgag gtacgatcaa
    18721 gaccaataaa gagaccggtt tgagtgacat agctgaccaa acggaaattt ccagaagcta
    18781 aagcaattcg actcatcgta tcttctaccc gatacatctc cgcattgctt tccatcatga
    18841 ttttcccagc gagtaaacaa gtgtccagca acagatctaa ttcttttccc gtcatagcat
    18901 cttctccaat aattgattga ttagttttat tttactacaa aacataccag aagcgtaaag
    18961 aataaaaaaa cttgaggaag gaataaaaga ggcgtatact gtttttgtat tgcgggtgta
    19021 gtttagtggt aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc ccgtcacccg
    19081 cttaaaatag gttgtgaaaa agctgctctg caacaagtaa taagaacagc caagcaaaaa
    19141 caacttctta tgtttttaac ttggatggac ttattaccgc agatgcaagc ttttgcgcac
    19201 cgtttaccaa gcggttgtga aatcagtgta ttgtcttgag tatgaagaaa atttttgaat
    19261 aataataaat accttacaga tagagtggtg actgattaga gacgaggaat ccgtctcttt
    19321 tttgtatttt taagccatct taaaacaggt tttatttttt gctgtttcta tttctttcta
    19381 gtatttattt ataggggaac atatgttaag atttagggag ctgttagtaa aggagtaaag
    19441 aaatgagcaa aaaaacaacc aaggcgatcg tggggatcgt cgctgctgtg attgcagggg
    19501 cattcggtgt ttccaccaca caaaaagatt cagatatctt tcagcagatt acaactattt
    19561 ttgattccac accacaagct tctcaagtag cggatgtgcc agaatatgat ggaaaacatc
    19621 aagaaataga aatcaatggg aatcagccgg aatttacaaa agaggatctt agcctttcca
    19681 aaggaacatg ggaaagctat tcaaatattg ataaattgaa cagagtagga caagccaatg
    19741 ctatgcttgg aaaagatttg ttccctactg aaaagagaga gcctttgtat attgacccga
    19801 caggttggaa acaaaagaaa ttaagtgacg ggcaatggtt atataatcgc agccatttaa
    19861 ttggtttcca gctgactggt caaaataaca acattaaaaa cttgatgaca gggacacgtt
    19921 cgttgaatgc tccatacatg cttgctcacg aaaatgatat tgcggcatat atcaaagaaa
    19981 caaatcatca tgtacgctac cgtgtcactc ctcattttga ggggaatgaa ctggtagcca
    20041 ggggagtgca attggaagca gaaagcattg aagacaaaaa aattgaattc aatgtcttta
    20101 tctacaatgt tcaagatggc tacacgatca attatgagac aggacaagca acaaaaacaa
    20161 aaaattagta gcagctattc gaatttttta tgaatattat gaaaaaattg tgtaaaaaca
    20221 tacattcaaa gaaatatagc gtatacttta tgaaaagagt acttttgata aagctacaga
    20281 aaaaaggaaa aaaacataca aatagtgttt cttaatttat tcggaagttt ggaatatttg
    20341 tttgtagttt tccctttctt cgagtaaaat aaggttgtag aaggtaagct ggaattacct
    20401 tgcgggaaag aggtgtagaa tcatgggttt tacagatgaa accgtacgtt ttggttttga
    20461 cgatagtcgc aaaaaagaag tgagcgaaac attagcgatt gtttatcggg ctttggaaga
    20521 aaaaggctac aatccaatca atcagattgt cggctacttg ctttctggag acccggccta
    20581 cattcctcgt tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg atgagatcat
    20641 ggaagtaatt gtcaaggact acctctcaaa tcatggagtg aatttatgag agtaatgggc
    20701 ttggatgtcg gttctaagac agtaggtgtt gctatcagtg acccctttgg ctggacagct
    20761 caaggagtag agatcattcg gatcaatgaa gcagaagaag agtttggctt tgatcggttg
    20821 aaggaattag tagcacaata tgaagtagac cggtttgtag ttggattacc taaaaatatg
    20881 aacaattcca tcggaccaag ggcagaagct tccatgtcat acggagaaaa gatcaaagaa
    20941 ctattctcct tgccagttga ttatcaagat gaacgattaa ccactgttca ggcagaacgt
    21001 atgctagtag agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat cgataaatta
    21061 gcagctgtga tgatcttaca aaattattta gacggctcag gtaagcaatt attttaataa
    21121 taaaagaaaa gaggtaatct tatgacagaa cataaccatt cccacgacca tgaaggacac
    21181 gaacacatca cattggtgga tgaccaagga aatgaaacat tatatgaaat cctactaaca
    21241 gttgacggac aagaagaatt tggacgtaac tatgttcttt tatatcctgc tggtgtttca
    21301 gaagatgaag atgtagaatt acaagcttat gcatacatcg aaaacgaaga tggaacagaa
    21361 ggcgagttag aacaaatcga aacagacgca gaatgggaca tgattgaaga agtattcaat
    21421 acattcatgg cggaagaaga agaatagact ataaagctag gagaaacctt gatatgatag
    21481 ggtttctcct ttttttattt tttactgtat gccatattgt atgccatcta gtcaatattt
    21541 aagtgttgta ctaatttttc aacagtcttt tctttcgttt cttttgccaa gtgaccgtaa
    21601 caatcgatcg tctgactaat ttttgtatgc cgtaatctag tttgaacttc taccatagta
    21661 gctccagcgt ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac accgagaaat
    21721 ggaaccatag ctttttcaca aatagtaatc aatcttgcat aaatagcgtg gtgtgtgatt
    21781 agttctttat agataccaca aaaaatcata gtcggatcag ttacaccaag tgtcagcata
    21841 tatttccgct gattaatctg ccattttttt aatatagaaa ttgttctttc atctagtgga
    21901 agaaccccag cactacaatc agttttagga tcactgattt tatagccatt tttggattgg
    21961 cttagtgttt tattgatggt gaccgtattt tcttgaaaat caatgtctga ccaattcaat
    22021 gcaagggctt cactgatcct aaccccgcta taaaagagaa agcgtagtaa cgttttatca
    22081 aagtcgtttc gataagtttc aggtttgctg tttaggtaat caaacaaggt gatcagttgt
    22141 tctttactgt aaactttgac cttttcttta tgcttagtgg tggttctttt aggcattaat
    22201 acgttgtcta gtggattagt ctcaataatc ccaagattga tagcgtagct aaaaatacgc
    22261 ttagaagttg aatgaagtac cttgtattgg tcagtagttt cagcccagct atttacaatt
    22321 tgttgggctt tttttaccgt gacttcattc acacgaagat ttccatattt tggaagaatc
    22381 cactttctaa acttgatttc agttttcatg aaggtggctt ctcggacagt ggttttatat
    22441 gtttctagcc acaagtcata aacttcttta aacgtttcgt tgttcgcttt tttaataccg
    22501 tttttatcgt agtccacttg caagcgtgac aaggcaagct gggcttcttt ttttgttttg
    22561 taacctcgtc tacgaacctt atcaggtttg ccagtcattt tgttcattcc aagataggct
    22621 tcatagagcc aaaatttacc ttttttagtt tcatattgct tgaatgttgc cataatagtt
    22681 ttccctccat agtgtagcaa gccgtggggg cggttggagg aggcagttta ttattatttt
    22741 gaatagtgtt tatttagctc agtaacaaga gctgtgaact cttttagaag atcattttca
    22801 taaaattctt gatactcttt tttctcatct gtattttttt ctgaatcatt aattaatgag
    22861 tcatatcgta aaagcgtatt tagaagagct gcgagataag caattggggt actcccgtca
    22921 ggaaattcaa ttcgagatcc tgttttaaga tattttattg cgtttgttaa ataaatcttc
    22981 tgtgagacag tcaggttatc ttcttcgatg ttattgatag cattagaaag attagaatca
    23041 attatagagt tatttttttc aatagttttt ttcaattgct tttcaagcac atttcgctta
    23101 atttcgggca tcatatgcat atctcttaat gtataagatc cgtttaatag ataggtagtg
    23161 gatacatcta atacctttga aagttgttta agcctggtgt tattgggaag attatagcca
    23221 ttttcccaat tactaacggc acctttactg gccggtgggg ataatttacc tccaagttgt
    23281 tccattgaga gtcccttttc aattctagcc agtctaattc tttgtcctgt ctccactgct
    23341 tcggggtatt tttttctagc cacattttca ccaccattca tatattttct ctactatatc
    23401 acgatttaaa tatgtgtgaa acataaaagt tcaaaaaagt atgttaaaat tcttgcaaat
    23461 gaaaaggtat gatggtatat tgagtttata agaggtatag aaatgtattc aagagcctct
    23521 tgcatttttt taaaatcttt gtatagaaat gtatgctaac gaggggggat aaatatggaa
    23581 ttaaaaattg acaaactcac gcttgatgat ccagctttta aaattattga cgatcgagtt
    23641 gttcaacaag tagagcaaac tcaaggaacg ttactttcat tattagaaaa aaagtaccag
    23701 tttccaaaat atatgaacaa ggcacaagct gctaagtata tgaacgtttc atacaatact
    23761 atgatgcaga aatatgttcc taacggttta aagttgatta tagttgatgg agtcgtaaga
    23821 atttcacaag atgaatgtga tcgctttatg gaagaatacc aaaaataaac gcaagccgtg
    23881 ggggcaaagg gattaatagg aggaaataat aatggaatcg acacaaaata aagcaatcga
    23941 aaaagtatta tcaacaattc ttacagaaga cacagcggca gaattagcaa acttggaagg
    24001 caaagcgcta gaagaaacgt ttgaatgttt gtacgaacaa atggactatc aaaaattgct
    24061 accacaagaa ccaacggtaa gcggagtatt gcgtggcttg aacgatttag tacaagcaga
    24121 gtttaaagaa cgactttcta ttgaagagta tcaagaaatt ttgtatcagc aagttgagtt
    24181 gttagcgagc ttgctagggc ttgaactgga ggacgttgaa tgaaaactga aatttggaac
    24241 ggacacatta taagatttgt cgatattaat gatgaatggt gggcagtggc gaaagatgtt
    24301 gcggaagcgt taggtcttaa acaggttact agagccattc actctttgcc taaagatggg
    24361 gttactacta gtaaggtcat cgattcattg ggcagaacac aagatgtaaa catcatcaac
    24421 gaaaaaaaat atttaccgca tggcattcaa aagccgtaaa aaagaagccg aggcatttca
    24481 agactggatt ttcgacatca tcaaagaact acgccagtcc acaggacttg aaggctttca
    24541 agtattccgc atgctagaca aagaacacca aaaagaagca atgactaaat taagccacgc
    24601 tattactgaa ccaaaaccag tagactacat taaagcgaat gtgatagcca ataaagctgt
    24661 atcaactatt tatggtcatt ccaaaatggt aaaaaagaaa gatatgactc ctgaaatgtt
    24721 ggttgatcgt gaacctattt tagatgagac agtagaatta atgactgtta aagaaaaata
    24781 tgggttgcag ttttcagtga gtgagaaaat ttataatcgt tctgctgaat tgcagacaac
    24841 ttaggagcat taacatgatc gagaacaaag aaaaagcctt atccataaca gtcttggcgg
    24901 acaggataaa ggcaacttaa ttgggataaa acccgatatt ctttgttccg attataccat
    24961 attaaaaaat cggagggaac cattatgaaa cgaaacttaa ttataggaag cattttaatc
    25021 ggcggaattt tcggcttatt accattaatt gcaaaaatag ctatctttgc aggattagcg
    25081 ttatatctct tcactgctta tgacgagtac gactatcaat taagaactgg agggcataag
    25141 tagatgtaca aaacaaaact attaaaccaa ctcgatagcc ttgaattaga ggaaattaat
    25201 caagggatcg ctgaactaga aaataatatc gggaaaacct actttggaaa ttcattcaat
    25261 gaaaaactaa cagttttgta tgtactcaaa aaacatgcag aacacaaaat aatttgtcga
    25321 gaaatcaacg agctaaaaaa tcagatttta accgcatggt taaacattac tgatatgcaa
    25381 gaagcaaggg tgaaaacatt taatacatgg gtaaagtatc agaatcaatt aaaaggtgct
    25441 gaatttgttc gggatgggtt gaaatacgaa cttgagcagt tgaagctcat ggaggtgtct
    25501 gaatgattta taccagtatt ttgaaaaagg aggagaggtt tattggcaaa taattatcaa
    25561 gcagcaacgg aattacaaaa gtcgggatgg tcaatttatc ccttatctcc tggaactaaa
    25621 attccaataa aaggctctag tggttggaat gatgcaacta acgattctaa tcaaattaat
    25681 gaatggtgga cggaagatgc aactagaaat attgggttaa tgcttgaaga tcatcaaatg
    25741 gtggtagttg acctagatca acattctgaa acacgaaacg gcgttgagaa ttataaaaaa
    25801 cttgcttcaa tgtatgatcc atttccttct acttatacgg aaatcacacc aggtaaagga
    25861 atccatttct tctttaaaat tcctgatggt gtaattatcc aacaagaaac aggtgcattt
    25921 gctgatatct tcgggcgtga tgataaaggt aaaagtttaa gcgggattga tattatcact
    25981 aaaggagttc caatagcgcc aactatgatt atttcaagaa gtgttgggag agcatatgaa
    26041 cctattgaaa acgtatctct aaataatatt gcaccagctc ccgattggct agtgaaccta
    26101 ctgctaaaaa aaccgcaaga aataaagact caataccgac ccgtagcaaa aacagggaca
    26161 gcaaaaaaat tagatatgat tgttcaaggc gctggcgaag gtagtagaaa cgatcattta
    26221 actaagttat gcggttggtt actatggcac ggtgtagata atcaaacttt agcagaattg
    26281 atttatacag caaatgcgta caatgaacca ccgctaaaag ataaagaagt gaatcaaatt
    26341 attcgatcga tgattaaaaa agatttgagg gggaaagctc atggctcata agcataaatt
    26401 agatagtatt ttagactttc ccgaagccag cgagcgtgaa gacaatatca ttgaattgaa
    26461 aacctggatg tcacgattaa gatgcaataa agacgatcag attaaaagta acagtgttgt
    26521 gaatgcagaa ttgattttaa ccaatgatag caatttagct ggaacaattg cttataacga
    26581 atttagcgga tacattcacc tattaaaaga ttctccttgg atcaatcgta ccgctggaga
    26641 atgggaagac agttttgagg atgccttaac ggcatatatt gaagaaaact acaatgtagt
    26701 ttttgatgac aacaaaatac acaaagcagt agtaaatgtg gctagaaaga atgttttcaa
    26761 cccagtaaaa gaacgtatcg aaaaagttaa atgggatcaa cagcctagac tggaaacact
    26821 gtttattgac cttttagggg tagacgacaa cctttacaca cgagaagtaa caaaacgttg
    26881 gatagtcgga tcagtcgcac gtatctataa accaggaata aagtttgaga tcgttccagt
    26941 tttggatgga ccgcaaggga ttggtaagag taccgttcca gcattacttt acacggatga
    27001 ttttttcacc gattcattag attcgctagg tgaaaagaaa gatgattata tgcagcttca
    27061 aggcaatgta attattgaac ttggagaatt atcttcaatg aacaaaacta aaattgaaca
    27121 gataaaaaac ttcatcagtg ccaagatcga taaaattaga cctccttatg gtcggaatgt
    27181 catagcttgg gcaaggcaat gtgtatttat tggaacgtcg aatgatgggc agtatttgaa
    27241 agatgataca ggaaaccgcc gtttttatcc tttgccttgt aagaataagc caaagatgaa
    27301 tccgtttaaa acaaaggatg aatattttct tcaagtgtta gctgaagcaa aggtgctttt
    27361 tgataaagga caacgaatct attttagtcc cgatgaagat aaagaagtct tagaaattgc
    27421 aaaagactat caagaggatg ccaagatgga gaatccaatt aaagaagcaa tcactaaata
    27481 tttggaaatg gaaatcccgt ataaatggga agaagctcca gcgtgggcga gacgttctta
    27541 ttatcagaac tatcctgaca gtaaatgtaa tgagaaaaat ttacagcatt tctataatgg
    27601 agctactatg caacaagagt tttatttagt tgatagcgta ctcaccgctg atattttgga
    27661 agctgttttt gataaacaag caaaggattt attgaatggt cgatcagatg cagaaactaa
    27721 aaagattgcc ttgattatta gcagtattcc tggttgggaa cggaagcaac tgccaaaaag
    27781 aaataaacga cgaggatttt acaataagtc aaatgcctat gaaaataaaa atcgagctgg
    27841 aaagtacaaa aagtaacggg tagtaacggg tcagtaacac ctagcccgtt actagtttac
    27901 ccttactgcc ccaagggata cagcaatttg gtaacggggt aacaccttat ttccttaaaa
    27961 cttttgaaat agttagctaa tatgctgggg aataatgtta ctggatttta cccgttacct
    28021 gttaccaaaa cgctagaacc cttgtccccg ttgggattga gtggtaacgg gtacctgtta
    28081 cgtaggtgtt accacgtgtt acgaatcaag gaggaaagta aatgctcaat attatttcaa
    28141 caaacaaagc accgaacttt caatatacag atgaaatgga tcggtttcta atgaacacgc
    28201 tggcatttag tgtgggattg gtaacagaag attattccac atttgatcca gaagtattaa
    28261 agataatgga agaagaacct gattggctgc aggagtcagt agcgtggtgt caatcgctag
    28321 tagtgggatc attggtagat agtggcaact atgatgatac aggtgagcta atggatgaat
    28381 ttaattgtct gcttaaccta tatgatcgag cgagacaacg agagcttaca tcgaatgagg
    28441 ataatttgtt tttaaacatc catgataagt tcttggcttt gctactaaca gatgatgaat
    28501 tgataactaa tttattggag gtagaataat tatgcatatg gaaagtatta gatacttcga
    28561 tggtgagaaa cacattgagt attcaaacac tcaagaagat gtagacttta ttagttttga
    28621 ggaagataaa atcactaaag ttaattttaa agacggaacg tgcttgaaga tagtttcgcc
    28681 ttatattgaa tacaaatcaa aatgggaaca agatgattct gacgaacgag atcgctttgg
    28741 ttggtaatct atggcagtta aaaaacaatg taatcatgct ggatgtaaaa cattgattga
    28801 ttataggcag aagtattgtg ggaagcataa agcaaagcaa acggcaatta agcgtgaaga
    28861 aagaaagcaa agcgaaggta aatattttca attctatcaa agtagaacat ggcgaaaggc
    28921 ttcatacttg tatcggttaa atcatccagt atgtgaggat tgcctagaag aaggcttgat
    28981 aaggaaagct gatgttgtag atcacaaaat tgaactaaaa gatgattgga atagacggtt
    29041 agatgaaact aattttcgtt ctttatgcca tgcacaccat aattccaaga cagccaatga
    29101 aagacaaaga cgagaaaaga gtaccctttg agttaagggg gcatagtgtg aaacttactg
    29161 acaatcgatg cctactcatt ttggtacaaa taaccgttgt gaaaaggcat aagggtaatt
    29221 acaaataacg attacacttg taatcacaag tgaaaaagta tataatgaaa gtaggagatt
    29281 tattgaaaaa tacaattgtt attactgata aaacagggaa acagcgcacc attgagttac
    29341 ctcattttgg tcaagtgacc attcaaatgc agaacggaaa aatcatttat atagataaat
    29401 tagataaaga aaaattctga tcgaaaaacg aaggaatgtg agcttaattg cttacgttcc
    29461 tttttctttt gtctgaaagg aggacaacat gggacaccct aaaatattac acgatacaaa
    29521 aggcaatata tcaagtgaag aaaaagcaat ccgtgtggac gctagagaag aattgttcaa
    29581 gcagcaacca ttaataaata tcacgccccc tgactggatg gcagcgagcg ctagaagtga
    29641 atggaatcgt atcgtaccaa cattaaaaaa agattatcca ttgagtgaag cagattatgg
    29701 ttcattggta gcctattgtt tagcctttgc tcgaatgaaa acagccgaag ccgagataag
    29761 aaaatcaggt acgtttatca catgcgaaaa cggagtaaag aaagccaatc cagcggttcg
    29821 ggttcaatca caagctatga gtgatttgaa aaaacaagcc acctcactag gtatgacctt
    29881 agaatcacga tcaaaactag ctttgaacaa ggttaaaaat gatgaacccg aagacccgtt
    29941 caaagagttg atgaattcat gaatgattac attgagaaag tcctatcagg taagctgatt
    30001 gcacccaaga aaattatcca agcgtgtgag cgtcatataa gcgatttgga gcgttctaag
    30061 tcagatagct tcccttatgt gttcgatgaa gaacaagcca ccaaagcgat taaattcatt
    30121 gagttgctac catctacgga cggtaaagca atcaagatgt taggatttca aaaattcatt
    30181 ctaggcagtc tttatggctg gcgtactaaa gaagggaatt acaggcgatt caatcgagcg
    30241 tttaccagta tgagccgtaa gaatgggaaa acgtatatcg caagtggcat ggctgccaat
    30301 gcgttgatta tggaacaaga accagcagaa gcaaggcaag tattgtttgt aagtaacgct
    30361 ttgaaacaag ctaaattggg ctatgatatg ctgtctaatt cacttagaaa cgtggtcaag
    30421 tctagtaagt ttttaagacc acaactgaaa attatgaact ctaagattca gcacttgccc
    30481 tctaattcgt tcgctatggc actggctagt gaaaccagca cgctagatgg gtttgcacca
    30541 acaaccgcaa ttcttgacga gtggcacgaa gcaaaaactc gtaaaacgta caacgtcatt
    30601 aagtcaggaa tgacccaaca aaagaacggc ttattgtgtg ttattagtac cgctgggctt
    30661 gatttaaacg ttcctatgta cgaggaatac ttattgttag atcgtgtgct aaaaggcgaa
    30721 gaacaagccg acaggtactt tatagcgata tgggaattgg acgatcccga agaaattcac
    30781 gatcaagaga aatggatcaa agccaatccg atttttgaaa gtgaagaaat caaaaaagta
    30841 atgattccaa ccattcaaga tgatgtgaac cttgctttga aacaaaataa ccttaattct
    30901 gtattggtga aaaacttcaa tttatggaga caagcgagtg aggacagcta tatgattgct
    30961 gaagactggc aggcaaccga agtagaacca caagatatta caggcaagcc cgtttatatc
    31021 ggagtggatt tatctaaaac agatgattta actagcgttt catggatcgt accgctagat
    31081 aacggcgaac tttattgtga ttcacatagc tttgtagcca ccaaatatgg gcttcaagac
    31141 aaagaaaagc gcgacggttt gccttatcga gaactagaaa aagctggtga gtgttccatt
    31201 actcaattag aaagtggaat cgtggactat gaccaagtat ttcagtttat tcaagatttg
    31261 attcaagaaa atgatttgga atgtatgggg atttgttatg acccgtataa cgctaattcg
    31321 cttatcagta aagctgaaaa agccaactac ccaatgttag aagtaagaca aggaacgatt
    31381 actctaaacg ttccgacccg aacttttaga gaacaagttt atgaaggcaa cgttattcac
    31441 aataaaaata cgattctcac ccatgcagtg aacaacgcta ttttaaaaac ggataacaac
    31501 ggcattcaga ttaataaatc aaagaacagt aacaaaattg atccaatagc tgcattaatc
    31561 aatgcctatg tgtttgcaat ggattacttc accacaacgg aaggagcgaa agcagacaat
    31621 gaattttata caagtgaaga attttctttc taattacatt cataccgttc ttttacttct
    31681 tggattggtg tgtgtgttgg ttgctatcac cttactaaca aatgtctact atggcttgtt
    31741 agcgctgggc atagtgctta ttggggtagc ggtcatgcta aatacagaac agaagggagg
    31801 ttaaaagatg gcatttttta aagcgagaca aaatacaacg ggagatcctt tcctacaaca
    31861 tgtggtatca atccaatcgg atgattacac caccagcttt acaagcgttc gtgcattaag
    31921 aaatagtgat gtgtttgcag ccgttcgaat tattgctagt gatattgctt caagtccgat
    31981 tcaattggtc aaaaacaata tgccacaagc tgataatgaa ctggtgaagt tactaaacga
    32041 gaaacctaat tcagaaatgg atggttggca tttaaagttt gctttggcgg tcaatatgtt
    32101 gctaaacggt aatagctttg cagagattaa acgcaacggt gaaaaggtag aagaaattca
    32161 cttattacct aactcaagtg taacggttac tcaattagat aatggtacgt tgtcttatca
    32221 gattggtgat aaaaaaagac gtgtgaagtc tagcgatatt ttgcacttca aatatttcac
    32281 ccaagatggt ttaacaggat taccaccact ttatgcgtta cgtgatgaac tgaaaattca
    32341 acaagctggc aaccgcacat tgcacaattt ctttactcgt ggtgtcagtg ggtcaggtat
    32401 tttgaaggtt cataagtcag atttagacgg atcagcaaag aacgcaattc gtgaaaagtt
    32461 tgaagaagcc aacggttcaa gtagtggaga taatgctctt agaacgatca ttcttgatga
    32521 aacaatggac tacaaaacat tagaaataaa caccgatgtt ttgaagctta tcaattccaa
    32581 tgattggaac acaaaacaaa ttgccaaggc gtttggtgta ccgattgagc gtttaggcgt
    32641 tgaaaatgaa cattctagca ccgttcaaag taatctccag tatatccaaa gtacgctgat
    32701 tcattacttt aatgtgtttg tgagtgaatt agatacgaaa cttgaaacca atatccgctt
    32761 taattccgat cagttactag agactgatcc tgaaacaaaa gtaaaaaatg tattggatca
    32821 ggtcaaaggg tcacttctca cgattaatga ggggcggtcg aaaatggggc taccccccat
    32881 ggatggtggc gatcgtttac tagcaagttt gaactttacg tatttagata cgttggagaa
    32941 atatcaatta aaagaacagg aaggagttac accagttgaa taatgaagaa gaaaaggaaa
    33001 aacggctgac agaagaagct gagctaacag ccgattctcc caaagtagag aaagaaaatg
    33061 aaaaacaacc aacagacggc aaaactattt caggctatgc gttgaaattc gggcaaccgt
    33121 caaaagattt aggcggcttt gtagaagtga ttacacccga agcattaaaa gaggtggatt
    33181 tatcaaatgt gttcttatta tatggacacg attattcaaa gccactagct agtgtcaaag
    33241 caggtacgtt gaaattgaat gtcgatgata caggattaca ttttgaagca gagctaaccg
    33301 acacgaccta ttcaaatgat gtgtatgaga atatctcaaa aggggttgtt gattctatgt
    33361 cctttgggtt cgtgcttgga ttagattcat tcgacaaaaa agaagatggc acaattgaac
    33421 gatcaataga taaaatcaaa gcacttaatg aaattagtgt cgtgaccgtt cccgcttatg
    33481 attcatcaaa tgtccaagta aataagcgtt cttacgaatc gtttatgagt aacaaccaag
    33541 caaaacaaac aaacaatagc ttagaatcca cttctaaagc acaaaaggag agtaataaca
    33601 tggaaaaaac tttaatcgat aacgaaaaaa cagaattgcg tgggtatgaa gaatatatcc
    33661 gttcacaagg cgaagtgcgt gatggagtca ctactgtaaa tgcagcggca gttgttcccg
    33721 aagaagtaat cggtgaagtc tttgatttga aacgttcaaa ttataactta gctcaatatg
    33781 caacagtaaa aacagtatca aatggacaag gtaaatatcc agtagcaact aaccaacaag
    33841 cagtgttagc aacaaaagct gaacttgctg aaattggtga tattgacgct gaaatgttta
    33901 cttcagttga ttataaagta gaaactcgtg ctggtaagat tgccttatca aatgaggttg
    33961 tggaagattc agcagtgaat attgtacaag aggtcaaaga tcagttagcg aaattggtag
    34021 aaaacactga caataagcat atcatggatt tattaaaaac attcactaag aaaacggctg
    34081 ctacgttgga cgatttgaaa caactataca atgtggcatt agacccagca ttaaataaaa
    34141 tggtaattct aaaccaaagc ggatattacc acctagatac attgaaagat tcagatggac
    34201 gttacatttt acaacccgat gtgacagcac ctagtggtaa atcattattc ggtatgccag
    34261 tagtattgat tgcagataca ttgtttgcca atcctaaagc tggaacattc cctatgatta
    34321 tgggggatat tgcacaatct atctttgttg ctcgtagaaa tcaagtaacg actcaatggg
    34381 aaaaattcga ttactactca caaggacttg cagtgatcgt tcgcaacgat tataagaaaa
    34441 ttgatcaaaa tgcttcagtg tatattgagt ttacgccagt tgtaacacca aaagtataga
    34501 aaaatattgg gcggcggttt atcccactgc ctttttttca ttaggagaga taaaatggta
    34561 attttggata gcataaaaaa aagtatgcgg atcgatcaca ctattgatga tgactttatt
    34621 caacaattga ttgatacagc aggcgaatat ataaaaagtg ctattgatag tagcgcaacg
    34681 gataaagata tggataatta tcagcaattt gatttggcgg tgtcattact tactcaacat
    34741 tggtatttga accgtcaaga agccagcagc gaacggatac cagtaacggt acaagcatta
    34801 gtacaacaaa tgagaggtgc ttattatgcc aatcattaag aatgtaaatg aattgactga
    34861 gagagtcaag tttaaaaaga caaaacgggt aaaagatgaa gatgggcaga tggtggatag
    34921 tgaagaaact gtatttgaat gttgggctaa tgtgcgttca caaatgctaa aagacgtgct
    34981 tgctagtgta ggtaccattc ttgaaggaac gttgacgttt attattcgat acgatcaaga
    35041 ttatgaacta acaaacgata tgaaagtagc ttggaaaaac aagagttata aaattatttc
    35101 aatcaatgaa ggaacggcgt ttaaagatta tacaacgatc atagctaaac agatttctta
    35161 gaatgattac acttgtaaac gttgtgggta tttagtataa ttaaggtagt aaatgaaggg
    35221 ttagctgcct gaattttgca agacttagct agtcgaaatc tattgttgga ctgaaaattg
    35281 taggtgtggt tgcaaacatg ctggactaag aaagatgata gatgatgaac gttcaatttt
    35341 aatacaggca tgctaggact gattggatgg ttttggcaag ctgattgtgt agaaatcaaa
    35401 gaacacgtct gttgtggacg tgtttttttt gttataattc aaaagaggtg atttaatgga
    35461 caaagcagag tttacaaatt tttattggaa gtattatcta catttagaaa atgagtttat
    35521 taataccaca gattttgtga tgctcgatga gaataatttc aaaacattct ctattgaata
    35581 tcaaaaacta ctactagcga ttggttcgga gtgtgagatt attttcaaag aactttgcgg
    35641 atttgatagc agtagcaata aaagcataac tgattttaaa gctgttatta aatctagcga
    35701 attaatgtgt ttggataatg gtgttagggt tatgaattct ttaacattga cttcactaaa
    35761 accttttgga gatgggtggc ctgaagatac accagattgg tggaaaatat ataacagagt
    35821 aaaacatggt cgttcttcaa attataagga agcaaatttg gaaaatgtgt tgtatgcttt
    35881 agcaagtcta tatttattag aggaatattt gcataagaaa gtaatactag aaggtgaagt
    35941 agactttata agtccagagt caaatctatt tacgttatct tgggaaagaa aacattcgtc
    36001 gatgcaaaaa gtgacgtttg aaaaagtgga ttcaaattat gtagctccac tcattgattt
    36061 taaagaacaa gaagaaattt aaaaaccccc acggcttatt gaagtttttg tatgccatat
    36121 tgtatgccat ttggtttaaa tcgcttgaaa aatagtgata gataactcta attcatttag
    36181 tcataaacgc tgttttgata acctttatag ttctttgata tgggtttctc attcatggcg
    36241 gaagaagaag a
//
LOCUS       DAXWLB010000007        34695 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXWLB010000007 DAXWLB010000000
VERSION     DAXWLB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.007"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..884
                     /locus_tag="ACGE4H_000296"
     CDS             <1..884
                     /locus_tag="ACGE4H_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFF8363742.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACGE4H_000297"
     CDS             932..3061
                     /locus_tag="ACGE4H_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFF8363743.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACGE4H_000298"
     CDS             3036..3725
                     /locus_tag="ACGE4H_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363744.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACGE4H_000299"
     CDS             complement(4133..4819)
                     /locus_tag="ACGE4H_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8363745.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACGE4H_000300"
     CDS             complement(4955..5149)
                     /locus_tag="ACGE4H_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363746.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACGE4H_000301"
     CDS             complement(5199..5639)
                     /locus_tag="ACGE4H_000301"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFF8363747.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACGE4H_000302"
     CDS             complement(5643..6488)
                     /locus_tag="ACGE4H_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFF8363748.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE4H_000303"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACGE4H_000303"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFF8363749.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8131..9333)
                     /locus_tag="ACGE4H_000304"
     CDS             complement(8131..9333)
                     /locus_tag="ACGE4H_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363750.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9320..11107)
                     /locus_tag="ACGE4H_000305"
     CDS             complement(9320..11107)
                     /locus_tag="ACGE4H_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFF8363751.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11120..12073)
                     /locus_tag="ACGE4H_000306"
     CDS             complement(11120..12073)
                     /locus_tag="ACGE4H_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8363752.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12258..12608)
                     /locus_tag="ACGE4H_000307"
     CDS             complement(12258..12608)
                     /locus_tag="ACGE4H_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8363753.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE4H_000308"
     CDS             12808..13887
                     /gene="pepA"
                     /locus_tag="ACGE4H_000308"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFF8363754.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14030..14350
                     /locus_tag="ACGE4H_000309"
     CDS             14030..14350
                     /locus_tag="ACGE4H_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFF8363755.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14372..14836
                     /locus_tag="ACGE4H_000310"
     CDS             14372..14836
                     /locus_tag="ACGE4H_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8363756.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE4H_000311"
     CDS             15032..15637
                     /gene="ytpR"
                     /locus_tag="ACGE4H_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFF8363757.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15676..16965)
                     /locus_tag="ACGE4H_000312"
     CDS             complement(15676..16965)
                     /locus_tag="ACGE4H_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFF8363758.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE4H_000313"
     CDS             17393..17872
                     /gene="rlmH"
                     /locus_tag="ACGE4H_000313"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFF8363759.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18225..18539
                     /locus_tag="ACGE4H_000314"
     CDS             18225..18539
                     /locus_tag="ACGE4H_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8363760.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            18555..18941
                     /locus_tag="ACGE4H_000315"
     CDS             18555..18941
                     /locus_tag="ACGE4H_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFF8363761.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            18970..20355
                     /locus_tag="ACGE4H_000316"
     CDS             18970..20355
                     /locus_tag="ACGE4H_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8363762.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            20358..20511
                     /locus_tag="ACGE4H_000317"
                     /pseudo
     CDS             20358..20511
                     /locus_tag="ACGE4H_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE4H_000318"
     CDS             20534..21739
                     /gene="mobT"
                     /locus_tag="ACGE4H_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFF8363763.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE4H_000319"
     CDS             22523..24433
                     /gene="ltrA"
                     /locus_tag="ACGE4H_000319"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8363764.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKRNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            24537..24761
                     /locus_tag="ACGE4H_000320"
     CDS             24537..24761
                     /locus_tag="ACGE4H_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363765.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            24878..25375
                     /locus_tag="ACGE4H_000321"
     CDS             24878..25375
                     /locus_tag="ACGE4H_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFF8363766.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            25462..25854
                     /locus_tag="ACGE4H_000322"
     CDS             25462..25854
                     /locus_tag="ACGE4H_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8363767.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            25838..28285
                     /locus_tag="ACGE4H_000323"
     CDS             25838..28285
                     /locus_tag="ACGE4H_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8363768.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            28288..30471
                     /locus_tag="ACGE4H_000324"
     CDS             28288..30471
                     /locus_tag="ACGE4H_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8363769.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            30468..31469
                     /locus_tag="ACGE4H_000325"
     CDS             30468..31469
                     /locus_tag="ACGE4H_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8363770.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            31484..32398
                     /locus_tag="ACGE4H_000326"
     CDS             31484..32398
                     /locus_tag="ACGE4H_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8363771.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            32712..32759
                     /locus_tag="ACGE4H_000327"
                     /pseudo
     CDS             32712..32759
                     /locus_tag="ACGE4H_000327"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE4H_000328"
     CDS             32775..34694
                     /gene="tet(M)"
                     /locus_tag="ACGE4H_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8363772.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT        11358 a         6339 c         7111 g         9887 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataacgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gatgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctat gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtatttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     8101 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     8161 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     8221 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     8281 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     8341 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     8401 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     8461 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     8521 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     8581 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     8641 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     8701 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     8761 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     8821 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     8881 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     8941 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     9001 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     9061 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     9121 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     9181 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     9241 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     9301 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     9361 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     9421 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     9481 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     9541 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     9601 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcataccat
     9661 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     9721 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     9781 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     9841 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     9901 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     9961 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
    10021 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
    10081 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
    10141 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
    10201 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
    10261 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
    10321 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
    10381 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
    10441 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
    10501 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
    10561 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
    10621 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
    10681 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
    10741 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
    10801 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
    10861 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
    10921 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
    10981 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
    11041 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
    11101 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
    11161 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
    11221 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
    11281 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
    11341 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
    11401 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
    11461 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
    11521 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
    11581 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
    11641 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
    11701 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
    11761 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
    11821 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca ttcccttttc
    11881 cttgacgtgg ttctttcttt acgattgctc cgccttgaac ggccaattcg tatgttcgat
    11941 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
    12001 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
    12061 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caataatacc
    12121 gtataataac tacaaatact agctattcca agaggaaata gccctatttt tataaaaatc
    12181 acccactata aaagaaggat aggatacctc tcttttagat agtgggtgcg tattcgttat
    12241 ttcatttttt tgacgtatta atctattgcg cccttttttc ttgaaatgtc taatacggtt
    12301 cctgtatgag catctgctaa aaattcataa taaacagttt cgccatcttc tatccgggaa
    12361 agcccacccc gataagcact cgaccgaacg gcgaatttcc gaataggttc tttttcaaaa
    12421 gagatccaag caccttcgat tgcgccttct tttaaaaaag atgttttgat attttcaagc
    12481 acttggtctg ctgataacac ttttttgcga tgataccaaa tcgttgaagc taccccactc
    12541 aataatccta gaccgatccc aacggctaac ccgcctttaa aatagcttaa ttcgctttct
    12601 tcatacatgt ggatatacct ccttcttctc acttgctact ctttgtgttt attttaccac
    12661 actctcatat tttctttgaa ataaataacc gctaccagaa aaaataagct ataataaata
    12721 cgatcggtct gcagaaaaaa tcatatttta tccgcagatc aatataagtc tgagaggagt
    12781 ttagtctgaa atttagacga atagcatatg gaagaaaaaa catttcaacg aatcaaagag
    12841 ttaacagaat tacaaggaac aagcggtttt gaacaagacg ttcgcgccta tatggaaagt
    12901 catatggaac cgctggtaga tgagttacag ttagatggtt tgggcggcat ttttgggctg
    12961 cgccaccatg aagaagcgga tgcacctcgt gtaatggtcg cagcacatat ggatgaagtc
    13021 ggattcatgc tgacacaaat ccaagaaaac gggcttttta aagtcgtacc tctaggcgga
    13081 tggaaccctt acgttgtttc tgctcaacgt tttacactta aaacctctac tggtaagaat
    13141 tatccatgta tttcttcttc cgttccccca catttattac gaggaacaag cggacaaaaa
    13201 tcagtagaag taacagatat tttgttcgat gctgggtttg aatcaagaga agaggcaatg
    13261 agttacggtg ttcttccagg agacacaatc gttccttacg ctgaaacgat caaaacagcc
    13321 aacgggaaga atatcatcag caaatcgtgg gacaaccgct atggctgtac aatggtatta
    13381 gaagctttag aagcgttaca aaatgaaaca ttaggtcata cattgattgc aggcgcgaat
    13441 gtccaagaag aagtaggcct gcgtggttct aaagcttctg tcaataaatt caagccagat
    13501 ctgttctttg cagtagactg ctctgcagca gatgacacag tgacgaaaaa tggcacattc
    13561 ggacatttag gagaaggtac attgatgcgt atccaagatc caggtttgat catgcttcct
    13621 cgtttgcgtg agtacttgct agatatcgct gaaacaaaca acatccctta tcaatacttt
    13681 gtttcaaaag gcggtacaga tgcaggtgct gctcatacac aaaatgaagg aatcccaagt
    13741 acagtgattg gcgtagtggg acgttatatc catacgcatc aaacgatgtt cagtattcgc
    13801 gattttgaag ctgcacgaga aatgttgatt caaacattga aaggtttaga taaatcaaca
    13861 gtgaacacga tcgtatacgg gaaataacgc gatagtgaaa ctataaaatc tggcaaaagc
    13921 aaccttttgt cagatttttt ttataaaaag aggatcaatg tttgttagtt cgaaagaaag
    13981 gtgtataatt taattgaaaa atacataaac agaaagtaga ggaaaaagaa tgattatccc
    14041 aaatagttta gaagaattag caggctacgt agaaactgga aaaagtgtat ttttctttac
    14101 tgccggctgg tgcggagact gccgttttat caaaccgcaa atgcctgaaa tcgaaaatga
    14161 cttttctgat tggcaattta ttgaagtaga ccgtgacaag tacatagatg tagccgctga
    14221 atggaacatt tttggtatcc caagttttgt cgtgatccaa gacggaaaag aattaggtcg
    14281 tctagtgaat aaagatcgta aaacaaaaca ggaaatcgaa tcgttcctag aaagtgtaat
    14341 aaaaggctga catctgttag gaggggtcaa gatgaatcaa gaatataaga acattttagt
    14401 aggtattgac ggtagtgaac aagcacttgc tgctttcaaa aaagcgttag aagtggctcg
    14461 ccgtaataat gggaaagtat atgtagcaaa cgtaatcgat cagcaatttt ataattttat
    14521 gggctatgct cctattgatc aaaatctagt cgatcagcaa actgaaacag ccaagcaaat
    14581 gatcgaagaa tgtaaagaat atggaaaatc tgttgattac acaaatatag aaggaattat
    14641 tgcatacggc tctgttcgtg aatctatggc agtcaaattg ccaaaaaaat atgcaatcga
    14701 tctaatcatg gttgggcagt ctggactaaa tgcagtcgaa cgctttatga caggaagtgt
    14761 agccagctac gtcatacgtc attcaccttg tgatgtcttg atcgtttctg acgaagaaga
    14821 acagccgagt aattaagcaa gtggaacttg tataaccttt ttaatcaaac agtaaaggga
    14881 ctgtgacaaa agtcttgtca cagtcccttg ttacgaataa atggtgttcc agaagaagct
    14941 tcttcggaaa taaagcatga aaaaaatctc atgcagtgaa tgcaattttt tgttaagata
    15001 aatctcagca aaggataatg gaggaaagaa catgattttt gcttataaca aagcacacgt
    15061 cggagacact ttgatggtta tcatagccga cgataaagga acagaaaaca ctgtcgaacg
    15121 taaatggaat gttgctcgga tcaaagatga acaaggtcaa gttgttgctt ggaacttttt
    15181 ccatatctcc gatcatctaa caattgaagg gaacggccaa gtaagcttaa cagatgaaga
    15241 attagcgaaa ttgaatcgat tgattaaaga agcaggattc gaagaacagc ttgaagctga
    15301 tcatgaaccg aagatcgttg tcgggtatgt aaaaacatgc gtcccgcatc ctgactctga
    15361 ccatttatcg attactgaga cagaagtgga caatgggaca gtattgcaaa ttgtttgcgg
    15421 tgcaccaaat atcgaagctg gacaaaaagt tgtcgttgca aaaccaggag ccatgatgcc
    15481 agatggaatg atgatctggc caggaacttt gcgaggggta gccagctatg gaatgatttg
    15541 ttctgctcgt gaattgcatt tgccaaatgc tccagaaaaa aaaggaatct tagtattgcc
    15601 tgaggatgcc gtagtcggag aagtatttcc aaaataaaga ggttgtgaca agtctttgtc
    15661 acaacctctt ttttattaat tttcactatt ttgattttgt tttaatgccg attgatcgat
    15721 cgtcaattca acagtagttg ttttttcttt cgagccttca tagtaagtga ttttgacaga
    15781 atctcccact tttttcttat acaaagcaga ttgaagttct acaccagaag aaacttcttt
    15841 gtcatcgatc ttagtaatga cgtcgtattg tttcaaccca gctttttctg ccggtgtagc
    15901 tgtttggaca tttgtcacga tgacaccgtt agttacagat gaaggaatct tcaagatttg
    15961 ttcttgttgc tgagtagaaa cggcagataa gtcgaccatt gtgataccta gagctggacg
    16021 tgtcactttt ccgtcttttt ccagttggtt gatgatattc actacatcgt tacttgggat
    16081 ggcaaatccc attccttcta cgcttacatt agaagtcgat tcagaagtac ttgcgatctt
    16141 acttgagttg ataccgataa cttgtccttc gatattcact aaaggaccac cagaatttcc
    16201 cgggttgatc gctgcatctg tttggatcgc attgatattg actgtttcgt tcgattcatt
    16261 cgtacttgtt acttgtcggt tcaaggaaga aatgattcct gaagtgacag aattcgcata
    16321 ttcagatcct aatggggaac caatcgcaat cgcaggttca ccaaccttca aagcacttga
    16381 gtccccaaaa gaggcaaccg tttcgacttt gtctgaattg atcttcaaca ccgctaaatc
    16441 tgaataggca tctgttccaa ctaattcagc ttttaccttc gtaccgtctt tcatcatgac
    16501 ttccaaacct tgctgtccat ctactacgtg attgtttgtg actacataag cagtattccc
    16561 gtcttttttg tagattacgc cgcttccttc actataagct tccaaattac tatcatctga
    16621 gctgctactt tcttcttgct gcccgaataa tccgccaaaa ccgcttgatt ggttttgact
    16681 ttgtaagttg ataacagaaa cgacagcgtc ttgaacttta tctaccgctt tggtgatatc
    16741 agaatctaca ttgactttga cattttccac aactgtttca ccggcagaat tttgattgcc
    16801 gcttgtagca gtagaactat tgttgccagt gcccatcgct gcataaaaaa tgccagctgt
    16861 cagtagtcct ccaacgatcc ctccaacaag ccccagaccc aacttacgcc agaggctatt
    16921 atttttattt tttttcatct ttggtgtcac attttttcta tccatgtgtt cctcctccaa
    16981 ttacattcat ttatctactg ttagtatagt cttatttgtt gactttagtc tactttgaga
    17041 ctttgaactt tttatgaaaa actttaggaa aaatcattgt tttcagatga aaatatttga
    17101 ttttcttttt ccacagatat tgtgtaaaac tacgcggata ggattggaaa aataagggat
    17161 aaaacatatc cacagcctgt gtataaagtg gataaattct tataaaacgt atgtttgcct
    17221 aatgaatgtg atgaaataag gtaaaacaac gctgttttct gtggataaac ctgtggatgt
    17281 tgtgcataac tgttggggaa atctttttaa caaaaatagt caaaataaac atatagacga
    17341 aaaaattgat tataataaac aaagtgagcg tgaagatcaa aggagagaaa acatgaatat
    17401 caaaattatt tcagtaggaa aattaaaaga aaaatacttg atccaaggta tcaatgagta
    17461 tgtcaaacgg ctgaatgcct atgcgaaaat cgaattgatc gaagttcctg acgaaaaagc
    17521 cccggagaat ttaagcgaag cgcaaatgag acaagtaaaa gaaaaagaag gagaacggat
    17581 cttagcgaaa atcaaagaac aagaatacgt ctatggctta gcaattgaag ggaaaaatcc
    17641 cacaagcgaa tcttttgcta aacaaatcga ccaattaggt atccaaggaa aaagccacct
    17701 tgtttttgtt atcggcggtt ctttaggact cagcgaagcc gtcatgaaaa gaagcaatgc
    17761 tcagatctct tttggcaaaa tgacgtatcc acatcaattg atgcgcctga tcctcgtcga
    17821 gcaaatctac cgtgccttcc ggatcaacgc gggggctcca taccacaaat aatgatattt
    17881 tttaagagaa aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact
    17941 ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat gagctttgat
    18001 acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt
    18061 taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag
    18121 tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat
    18181 caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg
    18241 tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg
    18301 ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg
    18361 atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt
    18421 tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa
    18481 tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa
    18541 gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt
    18601 ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt
    18661 cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca
    18721 tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg
    18781 tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg
    18841 ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag
    18901 ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga
    18961 ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt
    19021 cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg
    19081 aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac
    19141 attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt
    19201 gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca
    19261 aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt
    19321 aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga
    19381 cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa
    19441 ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag
    19501 ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct
    19561 attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa
    19621 tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt
    19681 atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg
    19741 aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa
    19801 gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg
    19861 aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac
    19921 ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc
    19981 gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta
    20041 atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt
    20101 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
    20161 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
    20221 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
    20281 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
    20341 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
    20401 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
    20461 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
    20521 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
    20581 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
    20641 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
    20701 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
    20761 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
    20821 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
    20881 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
    20941 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
    21001 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
    21061 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
    21121 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
    21181 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
    21241 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
    21301 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
    21361 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
    21421 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
    21481 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
    21541 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
    21601 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
    21661 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
    21721 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
    21781 tatgaatttt ggacaaaacc tttgtgcgac tcgttcctta gtgaaaagct aaggcactaa
    21841 taaaagaacg aaaaagtttg gttattagga gaactgataa tctgacaggt ggaatgatga
    21901 agtaacgctc tgaaacgcct gccctgagcc tccgacaagc attgtatgag gaaactcaga
    21961 atgtttgaag ctcggtaaag acggctgaaa gataccgtaa tagttgatga acatatatct
    22021 tctatcgaaa actatccaga ggtgggtagc gtatccaaca ggtcgggggt ctataaaata
    22081 tctatggtta gaatgttcta taaggaactg acgaaacagc gaatgtacgg gtctaaattt
    22141 gaactttgta gaaatgcaaa ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat
    22201 aggttatgaa atgccatata acgttacagg cggtatcaag ccagcaggct tatagctagt
    22261 gacctaagga tatatatgta tagataagat tatcggaacg aggaaaggta ttgggttctc
    22321 atcaagagaa taaactgacg aagaacaata agcagttgaa ctaatgctga aaagcagagg
    22381 gcgaacctaa gaaattcttg taatgagaat ggaggaatgc cctcaagtcg tgtatatatt
    22441 agaaaagtat tatgttgttt caataaggat tacgagtaag ataaaaaagc tcactcaatt
    22501 tcaagtaaag gagtgaagcc ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga
    22561 tttaagacac gctgaatatt acgaaatgca aaacaccttt gatgatttat atgccaaaag
    22621 taagaatgga gatattttca ctcatcttat ggatattatc ctatcgagag aaaatatatt
    22681 actggcatat cgaaatatca aagcaaatgc aggcagtaaa acagcaggaa cagacggtac
    22741 aataattaaa gatattggca aattgccagc agaaacagtt gtcaaaaaag taagatacat
    22801 tgttgcaggc agtccacatg ggtatcgacc taagccagtg aggcgaaaag aaataccaaa
    22861 accaaatggt aaaaccagac ctctaggtat tccttgtatg tgggacaggc ttatacaaca
    22921 atgtataaaa caagtattag aaccaatctg cgaagctaaa tttagtgaaa acagctatgg
    22981 ttttagacct aataggtcag ttgaaaatgc aataaaagca acctataacc gcctacaaat
    23041 cagccagcta cattatgtga ttgaatttga tattaagggt ttctttgaca acgtgaacca
    23101 ttccaagctg ataaaacaga tatgggctat gggaatacgg gacaagcact tgatattcat
    23161 tttaaaaagg atattaaaag ctccaatcaa aatgactaac ggtacaataa catatccaga
    23221 gaaaggaact ccgcaagggg gaataatatc cccactacta gcaaacattg ttcttaatga
    23281 gttagaccat tgggtagaaa gccaatggca ggaaaatcct gttacaaaaa actatgtggt
    23341 tcatatcaat aaaagcggta gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac
    23401 aaagttaaag gaaatgtata tggttcgtta tgcagatgat tttagagttt tctgccgcta
    23461 caaggaaagt gctgaaaaag ccaaaatagc aatcactcaa tggattgaac aacgattaaa
    23521 attggaagta tcacaagaaa aaacaagaat tgtaaatgtt agaaaaagat actccgactt
    23581 tcttggtttc aagattaaaa tgataccgag aagaaagaag ttagttgtta aatcacatat
    23641 aagtgataaa caattcaaaa accaaaaaga caagctggta tcacaggcaa agaaaattgc
    23701 ttctccctca aaagggaaaa cggaattaga agaaacaaga ctttataatt caatggtatt
    23761 aggtatgcaa aattattatc agattgcaac gaatgtaaat ctggattgta tgaaactgaa
    23821 tagagctgta atggctatct tcactaacag attaggtaca aacaaaaggg gcagattgag
    23881 aaaaacaggc agacaactta cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct
    23941 tcggtatgtt gctggcgagg gcgaacctat tttccctgtt ggatatatcc aacacagaaa
    24001 accaatggca aaggtgtatt tagcgaattg ctacactcta gagggaagaa aatttatcca
    24061 caccaatcta agtgttgata aattactgat gtaccagtta atgaaattat ctttggaaaa
    24121 tcgcacaata gaatacgcag acaatcgaat ttcgctattt tctgctcaac atggaaaatg
    24181 tgctataact ctggaagaat ttcaacaagc taatgaaata cattgtcatc atattgtacc
    24241 aactggagct ggtggaaatg acgattataa aaatcttatt ttggtaaaag aggcggtaca
    24301 ccgtctaata cacgctaaaa cagaagaaac aatacaaaaa tatatagctc tattgaagcg
    24361 aaataaagtc cagctcataa gactaaataa atatagagta ttagctggca atcaagaatt
    24421 aaacttgata taggtctact acataaatac ttttcaatat aaacacgatg gaacgcggag
    24481 tgcggtgaaa gtcgcacgct ccgtgtgaat ggggggaaaa gtttgagata tttataatat
    24541 caggaactta cctatccata tataactggt ttctatcaaa cgctcaatca ctggtgcttt
    24601 tagcaatcgt tgtgattggc ttgtatcttg gcttcaagcg tgagtttagc aaactgattg
    24661 gctttttaat tattgcgatt attgcggttg gcttagtctt caacgctgct ggagtaaaag
    24721 acattttact agagctattc aatcgcatta ttggtgctta aataaaaccg ttcttttgtg
    24781 gaatataagt ggttttctta tgttccgcaa aggaatggta caccaaacga agtgcggtag
    24841 ggatttttga atctctacaa agaaaggacg tgaatatatg gacgatatgc aagtctatat
    24901 tgcgaattta ggcaaataca atgagggcga attggtcggt gcgtggttta cctttcccat
    24961 tgactttgag gaagtcaaag agaaaatcgg cttgaatgat gaatatgagg aatacgccat
    25021 tcatgattac gagttaccct ttacggttga cgaatacact tctattagcg aactcaatcg
    25081 cctatgggaa atggtatcgg agttgccaga agaactacaa tcggagctat ctgctctgct
    25141 cactcatttt tcaagtattg aagaactaag cgaacatcaa gaagatatta tcattcattc
    25201 cgattgtgat gatatgtctg acgtggcacg ctactacatt gaagaaacgg gtgctttagg
    25261 cgaagtgcca gccagtcttc aaaactatat tgattatgaa tcctatggtc gggatttaga
    25321 cctttcggga acgtttatct caaccaatca cgggattttt gaaatcgtct attaaatctg
    25381 tcggtacatt cttactggca gattttcttg tttacggggt agtcaaaaga ctatccccat
    25441 ttttatgaaa ggattgatta catgaagaaa atacgaagct ataccagtat ctggtctgtt
    25501 gagaaagtgt tgtactccat caatgacttt agacttccgt ttcccataac ctttacgcaa
    25561 atgacatggt ttgtcgtgtc actctttgca gttatgatac ttggcaactt gccccctctt
    25621 tctatgatag agggagcatt tctcaaatac tttgggattc ctgtggcttt cacatggttt
    25681 atgtctacaa aaacctttga tggtaaaaag ccttatggat ttttgaagtc tgtcattgct
    25741 tatgcactgc gacgaaagct gacctatgca ggaaaaaaag taacgcttgg cagaaaccag
    25801 ccacaagaag ccattacagc agttaggagt gaattttatg gcatatccaa ttaaatatat
    25861 tgaaaacaat ctggtctgga ataaagatgg ggaatgttac gcttactatg agcttgttcc
    25921 ctacaattac tcatttctaa gtccggaaca gaaaatacaa gtacatgatt ctttcagaca
    25981 gcttatcgca caaaatcgtg atggcaagat tcatgcttta caaatcagta cagaatccag
    26041 catactttct gcacaagaac gttccaaaaa tgaagtcact ggaaagctca aaacggttgc
    26101 ctatgacaaa atcgaccaac agacagacgc tttaatatcc atgattggcg aaaatcaagt
    26161 ggactaccgt ttctttatcg gttttaagtt gcttctcaac gatcaggagt tttctatgaa
    26221 aagtcttacc gttgaagcaa aaaatgcttt tactgatttt gtctatgatg tgaaccataa
    26281 gctgatgggc gattttgtta gtatgagtaa tgatgaaatc ctgcgttttc agaagatgga
    26341 aaagctttta gaaaataaaa tctcccgtcg tttcaaaatc cgaagattag ataaggatga
    26401 cttcggctat ctgattgaac acctttacgg acagacaggc actgcctatg aagagtatga
    26461 gtatcctcta tcaaagaaaa agctggaaca tgaaacactg attaaaacct atgacctcat
    26521 taagcctact cgctgtctgg tggaagaaaa acagcgatat ttgaaaatcc agcaggaaga
    26581 cgaaaccgcc tatgtagctt actttaccat caacagtatt gtcggcgaac tggacttccc
    26641 gtcctctgaa atcttctact accagcaaca gcaatttacc tttccgattg atacgtcaat
    26701 gaatgtggaa attgtagcga atcgtaaagc cctatctact gtccgcaata aaaagaaaga
    26761 actgaaagac ttggataacc acgcttggca aagtgataat gaaaccagct ctaatgtggc
    26821 ggaagctctg gaaagtgtga acgagctgga aaccaattta gaccaaagca aggaatctat
    26881 gtataagctg tcctatgttg taagggtatc agcaaatgac cttgacgaac ttaaacgtcg
    26941 ttgtaatgaa gtgaaagatt tctatgatga tttgagcgtg aaactggtac gaccttttgg
    27001 ggatatgctg ggcttacatg aagaattttt acctgccagc aaaagatata tgaatgacta
    27061 tattcaatac gtgacctctg atttcctcgc tggtttaggt tttggtgcta ctcaaatgct
    27121 gggtgaaaat gaggggattt atgttggcta cagcttagat actggacgca atgtctatct
    27181 gaaacctgct cttgccagtc aaggggttaa gggttcagta accaatgcgt tagcgtctgc
    27241 ctttgtcggt tcgctgggtg gtggtaaatc ctttgcgaat aaccttatcg tctattatgc
    27301 agtgctttat ggggcacaag cagtgattgt agacccaaaa gcagaacgtg gcagatggaa
    27361 agaaaccttg ccagagattt cccatgaaat caatatcgtc aatctgactt ctgatgagaa
    27421 aaacaaaggc ttacttgacc cttatgtaat tatgaaaaat accaaagatt ctgaatcact
    27481 ggctattgat attttgacat tccttacggg gatttcctct cgtgatgggg aacgcttccc
    27541 aatccttaga aaagccattc gtgcagtaac caatagtgaa gtgcgagggt tgatgaaagt
    27601 gattgaagaa ttacgggttg agaatacgcc actaagtacc agcatagccg accatatcga
    27661 aagttttaca gactatgact ttgcccatct gctttttagt gatggttatg tagagcagtc
    27721 tatcagcctt gaaaaacaac tgaacattat acaggttgcc gacttggtac ttcctgacaa
    27781 ggaaacttcc tttgaggaat ataccacaat ggagttacta tcggtagcaa tgctgattgt
    27841 cattagtacc tttgcgttgg actttatcca tacagaccga agtattttca agattgtaga
    27901 cttagacgaa gcatggagct tcttacaggt ggcacaaggg aaaacactat ctatgaagct
    27961 ggttcgtgct ggtcgtgcta tgaacgctgg ggtatatttc gtgacccaaa atacagatga
    28021 cctcttagat gaaaaactga aaaataacct cggcttaaaa tttgcctttc gttccactga
    28081 ccttaacgag attaaaaaaa cccttgcctt ttttggtgta gacccagagg acgaaaacaa
    28141 tcagaagcga ttgcgtgatt tggaaaacgg gcaatgcctt atcagtgatt tatatggtcg
    28201 tgtcggtgtg atacagttcc accctgtatt tgaagaactg ctccatgcct ttgataccag
    28261 accacctgtg cgaaaagagg tgtaaatgtg aaaccatcaa tattaaacag aataaaatca
    28321 aactggacgc tgaaacgtct aggtaaagtg gcaatgacag tggctttcac acttgtgatt
    28381 gccatttttc ttttagccat gctgggaacg gtggttcaag ctgcgggctt ggtagatgat
    28441 acggtcaatg tggcaaatga atacagccga tacccacttg aaaactatca actggatttt
    28501 tatgtggata atagctgggg ctggctaccg tggaactggt cggacggaat tggaaaacaa
    28561 gtcatgtatg gactatatgc cattaccaat tttatttgga caatcagtct ttatgtttcc
    28621 aatgcgacgg gttacttagt acaggaagcc tattccttag acttcatttc cgctacagca
    28681 gattccattg gcaagaatat gcagaccctt gcaggggtta cgcctagtgg actatcaaca
    28741 gagggtttct atgttggatt ccttttactc ttgattttgg ttcttggggt ttatgttgcc
    28801 tatacaggac tgataaagag agaaaccaca aaggcaattc atgccctcat gaattttgtg
    28861 ctggttttta tcctatcggc ttcctttatt gcctacgctc ccgactacat taaaaaaatc
    28921 aatgactttt catcagacat cagcaacgct agtttgtcac tgggtacaaa aattgtcatg
    28981 ccccattcag atagtcaagg caaggacagt gtggacttga tacgtgacag cctattttcc
    29041 atacaggttc agcaaccgtg gctactgctt caatataaca gttcagacat tgaaagtatt
    29101 ggtattgacc gtgtggaaag cctgctctca accagtccag attccaacaa tggggaagac
    29161 agagaaaaaa ttgttgcgga agaaattgaa gatagaagca ataccaatat gacgattaca
    29221 aagacgatta accgtttagg tacagtcttc ttcctatttg tcttcaatat tgggatttcc
    29281 atatttgtat tcctattaac aggaatcatg attttctcgc aggtactttt tatcatctat
    29341 gctatgtttc tgcctgtgag ctttatttta agcatgattc catcatttga tggtatgtca
    29401 aaacgagcca taacaaagct ctttaatacc attttgacac gagctggaat cacattgatt
    29461 attacgacag catttagtat ttcaaccatg ctctatacct tatcggctgg ttatccgttc
    29521 tttttgattg cttttctaca gattgtgacc tttgcaggaa tctactttaa gctgggcgat
    29581 ttaatgagta tgttttctct acagagtaac gattctcaaa gtgtgggaag tcgtatgatg
    29641 agaaaacctc gtatgcttat gcacgctcac atgcaccgtc tacagcggaa acttggacgc
    29701 tccgtgactg ctttaggggc tgggtctgcc attgccagtg ctacgggtaa aaaaggacag
    29761 tcgggttcgg gaagttctgc gagtacacaa gcagatcact ctcgaccaaa cgggcaggaa
    29821 aaatcaacac taggaaagcg tatcggtcaa accatcggta cagtagctga taccaaagac
    29881 agaatggtag atactgctgg taatttgaaa gaacaggtta aggatttgcc gaccaatgca
    29941 agatatgcag tatatcaagg aaaatctaaa gtgacggata atgtccatga tttaaccaga
    30001 agtatttctc aaaccaaagc ggataaagcc agtggacgca agaaacagca ggaacaaaga
    30061 cggaaaacca ttgcggagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca
    30121 gcttcttccg ttcatgaaag accagccact aagcaagaac aatctcatga tggacagact
    30181 tcaaggcaaa caactgtaca ggcttcctat agggaatctc aacaagccaa acaagagcgt
    30241 ccaacagtta agtccgattc ttcaagttta aaaacggaac gtcaaagtaa tacagttcaa
    30301 gaaagaacgg ttcaaaagcc agtaacttca actgcaccaa cagatagagc ttcacaacgt
    30361 tcaatcacaa aagaacgtca gtctactgtt caaagagtac cactacaaaa tacaaaaagt
    30421 agaccaccaa tcaaaaccgc caccattaag aaaggcagta agaaaccatg aagctgaaaa
    30481 ctttagtgat tggtggttcg ggattattct tgatggtctt ctcactgctt ctgtttgtcg
    30541 ccattttatt ttcagatgaa caagatggcg gtttttccaa cattcactat ggcggtgtgg
    30601 atgtttccgc agaagtgctg gctcataagc ctatggtaga aaaagttgcc aaagaatatg
    30661 gaattgaaga atacgtcaat atacttcttg cgattataca ggtggaatcg ggtggtacag
    30721 cagaagatgt tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag
    30781 aagaatccat taagcaaggt gtgaagtatt tcagtgagtt attagccagt agcgaaaggc
    30841 ttggtgtaga tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttaggat
    30901 atgtggctaa tcgtgggaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt
    30961 attcgggtga cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct
    31021 ggcgatacaa ttacgggaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa
    31081 cagagtttga tgatgataca gtacaagcca tcatggacga agctctgaaa tttgaaggct
    31141 ggaagtatgt ctatggcgga gcttccccga ctacctcttt tgattgtagc ggactgacac
    31201 aatggacgta tggaaaagct ggaatcaact taccacgaac agcacaacag caatatgatg
    31261 taacccggca tatcccatta ttggaagcaa aagccggcga tttggttttc tttcattcta
    31321 cctataacgc tggctcttat attactcata tcgggattta tttaggcgat aaccgtatgt
    31381 ttcatgcagg cgaccctatc ggttatgccg acttaacaag ctcttactgg caacagcatt
    31441 tagtgggagc aggacgaatc aaacaataag aaaggacgat tcaatgatga aattcggaaa
    31501 aaatcagaat acagaaaaac agacaccaaa ggaaaagaaa cctcgtgtct ataaggtcaa
    31561 tcctcgtaaa aaggttgtga ttgtcctatg ggtactttta ggacttagtt tcagctttgc
    31621 gatattcaag cactttacag ccattgatac ccataccatt catgaaacca ccatcataga
    31681 aaaggaatat gtcgatactc atcatgtgga aaattttgta gagaactttg cgaaagtcta
    31741 ctattcatgg gaactaaacg acaagtccat tgataatcgc atagaaaacc tgaaagcata
    31801 tctgacagag gaacttcaag ctctgaatgt cgatacagtt cgtaaagata ttcctgtatc
    31861 gtcttctgta aaaggatttc agatatggac ggtagaagca gatggcgaca atcaatttga
    31921 tgtaacctac agtgtagacc aactcattac agagggggaa aatacaaaga ccgtccactc
    31981 tgcttatata gtgagtgtct atgtagacag tactggaaat atggtcctca ttaagaatcc
    32041 gaccattact agcataccaa agaaatcaga ctataaacca aaagctattg aaagtgaggg
    32101 tacggttgat tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct
    32161 ctatcctaca gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc
    32221 aatcggaaaa gagtacatct ttcaagaact ggtaaatcct atttacaatc gtaaggataa
    32281 tcaagtcacg gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt
    32341 atctcaattt gatttggtac ttgaaaagaa cgggagtaat tggaagatta tagaataaca
    32401 aatattggta cattattaca gctattttgt aatcacgtac tctctttgat aaaaaattgg
    32461 agattccttt acaaatatgc tcttacgtgc tattatttaa gtatctattt aaaaggagtt
    32521 aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagtgg gaacaaataa
    32581 ttggatgtcc ttttttagga gggcttagtt ttttgcaccc agtttaagaa tacctttatc
    32641 atgtgattct aaagtatccg gagaatatct gtatgctttg tatgcctatg gttatgcata
    32701 aaaatcccag tgataagagt atttatcact gggattttta tgcccttttg ggcttttgaa
    32761 tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcgggaa
    32821 aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg
    32881 tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa
    32941 ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc
    33001 caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa
    33061 ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac
    33121 ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg
    33181 atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt gtaatcaaac
    33241 agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct gaacaatggg
    33301 atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt aaatcattag
    33361 aagcattgga actcgaacaa gaggaaagca taagatttca gaattgctcc ttgtaccctg
    33421 tttatcatgg aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga
    33481 ataaatttta ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa
    33541 ttgaatattc ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc
    33601 atttgcggga ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata
    33661 cttcaataaa tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt
    33721 tgcaaaatga gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga
    33781 gagagagaat tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc
    33841 aacaaaggga aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac
    33901 aatattatgt ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa
    33961 tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc
    34021 ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag
    34081 tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa
    34141 gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg
    34201 cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg
    34261 attgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt
    34321 ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt
    34381 tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca
    34441 acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca
    34501 ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct
    34561 ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg
    34621 aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca
    34681 ataaaataac ttagt
//
LOCUS       DAXWLB010000008        33128 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXWLB010000008 DAXWLB010000000
VERSION     DAXWLB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.008"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            69..926
                     /locus_tag="ACGE4H_000329"
     CDS             69..926
                     /locus_tag="ACGE4H_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFF8363773.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1041..1961
                     /gene="pstC"
                     /locus_tag="ACGE4H_000330"
     CDS             1041..1961
                     /gene="pstC"
                     /locus_tag="ACGE4H_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFF8363774.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1961..2845
                     /gene="pstA"
                     /locus_tag="ACGE4H_000331"
     CDS             1961..2845
                     /gene="pstA"
                     /locus_tag="ACGE4H_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFF8363775.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2860..3666
                     /gene="pstB"
                     /locus_tag="ACGE4H_000332"
     CDS             2860..3666
                     /gene="pstB"
                     /locus_tag="ACGE4H_000332"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8363776.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3682..4440
                     /gene="pstB"
                     /locus_tag="ACGE4H_000333"
     CDS             3682..4440
                     /gene="pstB"
                     /locus_tag="ACGE4H_000333"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFF8363777.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4453..5130
                     /gene="phoU"
                     /locus_tag="ACGE4H_000334"
     CDS             4453..5130
                     /gene="phoU"
                     /locus_tag="ACGE4H_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFF8363778.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5707..6399
                     /locus_tag="ACGE4H_000335"
     CDS             5707..6399
                     /locus_tag="ACGE4H_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8363779.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6396..7163
                     /locus_tag="ACGE4H_000336"
     CDS             6396..7163
                     /locus_tag="ACGE4H_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFF8363780.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7263..8831
                     /gene="liaX"
                     /locus_tag="ACGE4H_000337"
     CDS             7263..8831
                     /gene="liaX"
                     /locus_tag="ACGE4H_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFF8363781.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9128..9448
                     /locus_tag="ACGE4H_000338"
     CDS             9128..9448
                     /locus_tag="ACGE4H_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFF8363782.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9448..9798
                     /locus_tag="ACGE4H_000339"
     CDS             9448..9798
                     /locus_tag="ACGE4H_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFF8363783.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9965..10903
                     /gene="hprK"
                     /locus_tag="ACGE4H_000340"
     CDS             9965..10903
                     /gene="hprK"
                     /locus_tag="ACGE4H_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFF8363784.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10918..11748
                     /gene="lgt"
                     /locus_tag="ACGE4H_000341"
     CDS             10918..11748
                     /gene="lgt"
                     /locus_tag="ACGE4H_000341"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFF8363785.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11792..12817
                     /locus_tag="ACGE4H_000342"
     CDS             11792..12817
                     /locus_tag="ACGE4H_000342"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8363786.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSRAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12832..13770
                     /gene="galU"
                     /locus_tag="ACGE4H_000343"
     CDS             12832..13770
                     /gene="galU"
                     /locus_tag="ACGE4H_000343"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8363787.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13895..14335
                     /locus_tag="ACGE4H_000344"
     CDS             13895..14335
                     /locus_tag="ACGE4H_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFF8363788.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14338..14940
                     /locus_tag="ACGE4H_000345"
     CDS             14338..14940
                     /locus_tag="ACGE4H_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFF8363789.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14999..16105)
                     /locus_tag="ACGE4H_000346"
     CDS             complement(14999..16105)
                     /locus_tag="ACGE4H_000346"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFF8363790.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16315..17316
                     /gene="ccpA"
                     /locus_tag="ACGE4H_000347"
     CDS             16315..17316
                     /gene="ccpA"
                     /locus_tag="ACGE4H_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFF8363791.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17653..19998)
                     /locus_tag="ACGE4H_000348"
     CDS             complement(17653..19998)
                     /locus_tag="ACGE4H_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFF8363792.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20277..21179
                     /locus_tag="ACGE4H_000349"
     CDS             20277..21179
                     /locus_tag="ACGE4H_000349"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFF8363793.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21392..23167
                     /locus_tag="ACGE4H_000350"
     CDS             21392..23167
                     /locus_tag="ACGE4H_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFF8363794.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23233..24819)
                     /locus_tag="ACGE4H_000351"
     CDS             complement(23233..24819)
                     /locus_tag="ACGE4H_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFF8363795.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            25070..25294
                     /locus_tag="ACGE4H_000352"
     CDS             25070..25294
                     /locus_tag="ACGE4H_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFF8363796.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25435..27186
                     /locus_tag="ACGE4H_000353"
     CDS             25435..27186
                     /locus_tag="ACGE4H_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFF8363797.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27186..28970
                     /locus_tag="ACGE4H_000354"
     CDS             27186..28970
                     /locus_tag="ACGE4H_000354"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363798.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29296..30147
                     /gene="licT"
                     /locus_tag="ACGE4H_000355"
     CDS             29296..30147
                     /gene="licT"
                     /locus_tag="ACGE4H_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8363799.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30243..32123
                     /locus_tag="ACGE4H_000356"
     CDS             30243..32123
                     /locus_tag="ACGE4H_000356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8363800.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(32163..33065)
                     /locus_tag="ACGE4H_000357"
     CDS             complement(32163..33065)
                     /locus_tag="ACGE4H_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8363801.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
BASE COUNT        10520 a         5880 c         7029 g         9699 t
ORIGIN      
        1 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
       61 gatttctaat gaaaaaactt aaattattct taccaataat aggattagct attcttttat
      121 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
      181 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
      241 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
      301 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
      361 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
      421 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
      481 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg cgcttcagga agcggaaccc
      541 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
      601 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
      661 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
      721 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
      781 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
      841 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
      901 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
      961 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
     1021 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
     1081 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
     1141 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
     1201 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
     1261 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
     1321 ttttatctgc tatcgttgcc acgccatttg ccattggtgc agcagtattc atgacggaga
     1381 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
     1441 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
     1501 ttggcgggac tgggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
     1561 cgactatcac aacgatgact gttgatgcat taaaggcagt gccacggcac tatcgggaag
     1621 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
     1681 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
     1741 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggtctt actactcctg
     1801 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
     1861 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
     1921 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
     1981 taaaattgct acaggtgttt tatatatagt ttcggggatc atcgtattga ttttagcagc
     2041 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
     2101 accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta actcgattta
     2161 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
     2221 ttcagaatat gctggaaaaa actggctaac agatgtgatt cgcacgtcga ttgaaatctt
     2281 gagctcgttg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
     2341 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
     2401 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
     2461 attggcctta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc cagaagcgac
     2521 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
     2581 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
     2641 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
     2701 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
     2761 cgtgttgatt attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
     2821 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
     2881 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatcta
     2941 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
     3001 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
     3061 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
     3121 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
     3181 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
     3241 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
     3301 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
     3361 gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga tatcttgctg
     3421 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
     3481 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcagct
     3541 cgtatcagcg actatactgc ctttttctat ttaggaaaag taatggaata tgacaaaact
     3601 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
     3661 ggttaagacg gaggcctcaa catgacaaaa gagattattt catctaaaga tcttcatctg
     3721 tattatggaa agaaagaagc actaaaaggg atcgatctaa gcttcaatca aggggaaatc
     3781 actgcgatga tcggtccttc tggttgcgga aaatccactt atctgcgttc tttgaaccgg
     3841 atgaatgacc tgattccgaa tgtaacgatc accggaagtg ttttatataa agacaaagac
     3901 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
     3961 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
     4021 ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
     4081 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggtgggcag
     4141 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
     4201 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
     4261 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
     4321 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga tacgaagaag
     4381 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
     4441 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
     4501 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
     4561 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
     4621 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
     4681 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
     4741 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
     4801 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
     4861 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
     4921 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
     4981 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
     5041 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
     5101 ttaaatacga accacaatga gattttctaa actgaagctg tgattatgcc gtttgtttag
     5161 gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag taataagaac
     5221 agtcaaacaa aaaatagctc ctcatatttt tcgtttggct ggacttatta ccgcagatgc
     5281 aagcttttga acaccgttta ttcgggaaag ggtttgtgac aaaacatgtc acaaaccctt
     5341 ttcatcattt catagaaagg ataaagaatg caaaaaaaaa ttagcatcat cactatattt
     5401 tttatttgtg tgcttacttc atgggttatt cagcatcaag ccgctttata tgaagtacct
     5461 gtcatcgaga tagaaaaagt acgaataaaa gaggatcatc aattgattac aggaaaactg
     5521 atgaacacgg atcaaaaagg agaatcgatt tatttatcgg ttccttatca aaaaaatcaa
     5581 atagaaacag caaatataaa agcgagacaa atgtatctag tccaaatgca tggagatgaa
     5641 tggcagctag tagcaaaaaa aaatgatggc tggcttttct tcttcacaag tatcttcatt
     5701 ggcgccatgt tgcttattgg tggaaaacag ggtctatttg ctttaggaag tctcatcatc
     5761 aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg gatttctcta
     5821 ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt ggctttagat
     5881 ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt ttttttagca
     5941 ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg ctatgaggaa
     6001 ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact gattggaaca
     6061 gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga attgactaag
     6121 cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt gggtcgagat
     6181 gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc agttccatcg
     6241 cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca gcatttatca
     6301 ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat ccctatctcg
     6361 gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt atacttgttg
     6421 ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat attcttttag
     6481 gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat cagaagtggc
     6541 cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc ttatttttta
     6601 tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc tttttatttt
     6661 gtttcctact attgcttcct atcactgatt ttttagcaat ccaaggtttt acatcggtag
     6721 aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga ttacttacga
     6781 gatcgttgct tctgattagt cttagtggtg ctgttttaga tgcgagtgta gcaatttcta
     6841 gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa ttgcgccatt
     6901 ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt ctttttgcat
     6961 tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt ccttttcagc
     7021 aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt accacattgt
     7081 ccgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt acaaaaaaga
     7141 aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt tttactgaat
     7201 cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag gaggcaattt
     7261 ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt tcgactgaag
     7321 aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa atcaaaaaag
     7381 aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa ttgatcgata
     7441 aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa agagaaagac
     7501 aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg aataaaactt
     7561 cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca cgcaatgaga
     7621 aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg acagaagaag
     7681 aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct agtggcgata
     7741 cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt aagaatcaat
     7801 ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat tggaaagatc
     7861 aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga tcacagttag
     7921 gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac aacatggaat
     7981 ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag catgaatttt
     8041 actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga aacgtgacgt
     8101 taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt tacggaaaaa
     8161 tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt aatgaagatc
     8221 atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc tacttaccta
     8281 aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg attgaaacat
     8341 tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg gaccaattaa
     8401 ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga aacgggaaca
     8461 tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg acagcagaaa
     8521 acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa gataatttga
     8581 aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca gatggcatcg
     8641 gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg tccaattacg
     8701 aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt cgtgtttcag
     8761 atggtgaaat cgcgcaagtg caactttcaa cgacaacagg aagtatctat ttaaaagata
     8821 cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga agcgattttg
     8881 taaaatagct tcccctattt tacaaaattg tgccttattg ccgatagtct atctcttgaa
     8941 caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag gaataatttc
     9001 agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt ttgcagaaag
     9061 acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag tagaacagag
     9121 gtgagacatg atgaaaaaaa agttgacaaa atctaataaa aatgttgttt tgacagggac
     9181 tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg tgctttatgt
     9241 attcctgagc ttagttgctt ttggaagtcc aattatttta tacattttat tagctttgat
     9301 tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat attatgaaaa
     9361 aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag cggaaaaaat
     9421 cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt agttaatacg
     9481 ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag cagtatttgg
     9541 acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa accgattttg
     9601 acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt tgttatcaat
     9661 gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag tttttcttca
     9721 ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat tgtcagcgaa
     9781 cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt ttttatttag
     9841 aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt tttaattggt
     9901 agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa atgaggtagc
     9961 atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt tggaaatcgt
    10021 gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct cacgcccagg
    10081 actagagttg actggttatt ttaattatta ttcccataat cgtttacagc tatttggcag
    10141 taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca tcatgagaag
    10201 attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccag ctccagaaga
    10261 aatgatccag gcagcaaata aaaatgggat agctgtactg cgttctccca tctcaacttc
    10321 tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc gaacaagtgt
    10381 ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg acagtgggat
    10441 aggaaaaagt gaaacagcgt tggagttgat caaacgcggc catcgattga tcgcagacga
    10501 tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc ctaaaatcct
    10561 ggaacatctg attgaaatcc gaggaatcgg gatcattgat gtaatgaatc tttttggtgc
    10621 gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag catgggcaaa
    10681 agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg cggaagtggg
    10741 cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca tcattgaagt
    10801 agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa catttgaaga
    10861 aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag tgacaatatg
    10921 ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta ctggtatgca
    10981 ttgatcatcg tatcaggtat cattatcgct atgtggctta gcagcagaga ggctgttcgg
    11041 gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc tatctccatt
    11101 attggcgctc gcttatatta tgttttattt gatcttcccc agtatatagc tgatccaatc
    11161 caaatattca atatccgttc aggaggctta gctatttatg gcggactgat tgcaggcgcg
    11221 atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt tttagatatc
    11281 gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt catgaaccac
    11341 gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt accgcaattc
    11401 atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct ttatgaatca
    11461 ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc acaccttttg
    11521 aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg tttctttatt
    11581 gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc tcaattatta
    11641 tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa aaaaggaaat
    11701 ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt tttagagcgt
    11761 taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg ttttaggacc
    11821 aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg acgttcggat
    11881 ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga atcaacgtta
    11941 tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg aagatgcagt
    12001 aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt ctgttgcaaa
    12061 agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta aaggattaga
    12121 acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag cagaaaaaag
    12181 aaaagctgtt gttgttcttt ctggaccaag ccgtgcagaa gaagtagcaa aacatgacat
    12241 cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac aagagctgtt
    12301 catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag agatgggggc
    12361 agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag ggtttggcga
    12421 taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt taggtgtagc
    12481 aatgggtgcc aacccgctga cctttatcgg attaagcgga ataggcgatc tagtcgttac
    12541 ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac aagggcagcc
    12601 cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata ctacccaagc
    12661 agcgatggaa ctagcccaaa tgatggaaat cgaaatgccc atcactcaaa caatctatga
    12721 agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt tacgtgatgg
    12781 aaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta tatgaaagtt
    12841 aaaaaagcag ttattccagc agcaggatta ggaacaagat ttttaccagc aacaaaagct
    12901 atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat cgttgaagaa
    12961 gcgttgaaat caggtatcga agacatcttg atcgttacag gaaaagctaa acgaccaatc
    13021 gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa aggaaagacc
    13081 gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat ccgtcaatct
    13141 catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg aaatgaacca
    13201 tttgtcgtta tgctaggcga tgatataatg gaagacgaag taccattgac acagcaatta
    13261 atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt tcctcatgat
    13321 gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg gttatacaat
    13381 gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt agctattatt
    13441 ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca accaggagcc
    13501 ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca acgcgtattt
    13561 gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt tatgaaaaca
    13621 agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga gtacatcctg
    13681 tctttaggta aaacattaga agcagaagga agcaagatcg atcataatcc aaaaggaata
    13741 acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt cagctgctaa
    13801 ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa aatgctatcc
    13861 ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa ttgcaggatt
    13921 gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt tgttgcaagt
    13981 ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga cgatcgaaat
    14041 cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttactgg tcaaaagtaa
    14101 tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt tcacagcagt
    14161 tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca tcgcgacgaa
    14221 agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag tcggtgggac
    14281 agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa aataaaaatg
    14341 gctaaaaaag gtggattttt attaggtgca gtagtcggag gcacagccgc agcaatcgcc
    14401 gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc tgcccaagcg
    14461 gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa atcgactgag
    14521 ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg tgatctagca
    14581 ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga atctgacgat
    14641 atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa aaaaacggca
    14701 gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac ttcagaagat
    14761 atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa tgaaggaatg
    14821 tcagaagcaa aaccagttgc agatgactca aaagaaatga tgaaaatggc aaaagaagat
    14881 gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac aaatcactaa
    14941 aatcacaaat ttatagaata aaaaggtggg agcggactag ctctcacctt ttttactctt
    15001 atttttctaa aacaagtaat tcttttggtg tagtcgtgaa tggcacacag ccatcgcttg
    15061 tcacatatac acagtcttcg atacgtaccc ctactttttc aggaatatag attcctggtt
    15121 caagagagaa gcacatccct tcttcgatta ctaagtcatt tccttctaca agggaaggga
    15181 attcgtggac ggttgttccg atcccgtgac ctaaacggtg attgaaatat tcgccgtagc
    15241 catagctgtt gatcacgttt cgtgcgattt gatcaagttc acctgctgtc acacctggac
    15301 ggacggcttc ttgtgcggca agctgtgctt caagagtgat gttgtagatt ttttcttgga
    15361 agtcggttgg tttttgataa gcaattgtac gagtagcatc actgcaatag ccattccaaa
    15421 caacgccaag atcgaataac acaagttcat tcggtgaaat cttcgtattt cctggtgtac
    15481 catgagggct tgcagcattt gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa
    15541 caccttgttt tttcaattgg tattcaattt ctgcaatgat cgcttgttca gtcacgcctt
    15601 ctttcactgc tttaaagcca atctcaaatg caacatcggc ccatttacca gcttcgatca
    15661 gtttgtcttt ttcctctaga gttttgatca gttgtagacg ttggatgatg gaagttagat
    15721 cagtgtaaga atccgttccc gtcaagatat cagagatagc atcgaagcgc tcgacagtca
    15781 aggcgttctt ttcaatccct acattgccag gctgaccata gcgagcacga agttcttttc
    15841 cgatgatctc ccaagggttt tcgctgtcta aatagccacg gacatcatac gcccaactac
    15901 tgttttgagc atcttcaact tccaaagcag gagtgaaaag gaaaggatct tgttcaagag
    15961 ggataaaaag agctagtaca cgctcatgag gatcactttc gtatccggaa aagtaagcga
    16021 tgtggccagg gtcgctgata taagccaatt cgacttggtg ttgtgccatc cattgacgta
    16081 gttcattgat ttttacgtta ttcatttggc aacctacttt ctaaattaat tttgtattca
    16141 ataattgtac cactgattta ttaaaagacc aacgttctaa aaactgaaaa aagtaaaaat
    16201 tttcatgaaa acagagataa aacggttgca attcttgaga ttatttgata ttctatttta
    16261 gaaatgtaag acgtattttc agaaaacata taaaatatga ataggagaac acaaatggaa
    16321 aagcaaacaa tcactatcta tgatgtagcg agagaggcaa atgtttcgat ggctactgtt
    16381 tcacgtgtcg taaatggcaa tccaaacgtt aaaccagcaa caagaaaaaa agtcttggaa
    16441 gttatcgatc gcttggatta tcgtccaaac gcagttgcgc gtggcttggc aagtaagaaa
    16501 acaacaacag ttggtgtaat catccctgat gtcagcaata tgttctttgc ttctttagca
    16561 cgtggtatcg atgatgtggc aacaatgtac aaatacaata ttatcttagc aaattcagat
    16621 ggcaacgacc aaaaagaagt caatgtattg aataacttac tggctaagca agtggacggc
    16681 gtgatcttca tgggtcacca tatcacagac gaaatccgtg gagaattttc acgttcaaaa
    16741 actcctgttg tattagctgg ctctattgat ccagatgaac aagtaggtag cgtaaacatt
    16801 gattatgtat ccgcaacaaa agatgcagta aacaaattgg caaaaagcgg caataaaaaa
    16861 atcgcttttg tcagtggtgc gttgatcgat cccatcaatg gacaaaatcg tctgaaaggc
    16921 tataaagaag cattaagcga aaataaccta ccatatagcg aaggattgat ctttgaagca
    16981 caatacaact tcaaagatgg tctatctcta gtcgatcgga tccataatag cggtgcaaca
    17041 gctgtctatg tttcagatga tgaattagcc atcggtattt tggatggttt attagaccgt
    17101 ggcgtgaaag taccggaaga attcgaaatc atcacaagta acaattcact actgacagaa
    17161 gtagctcgtc ctcgtttaac aagtatcaca caaccattat acgatttagg ggcggtatct
    17221 atgcgcctat tgacgaaaat gatgaataaa gaagaagtcg aagaaaaaac gattatcttg
    17281 ccttatggaa tcgaagaaaa aggatctaca aaataataaa aggacaacca ttcagaggtt
    17341 gtgtgcctgc cgtttaactc tgcgttacaa ggagcaattt caaaaaacag catctcatgt
    17401 ttgatgaata ttgtgactcg tgattcggag ttggcaggag aacaccgttt atttgggaaa
    17461 aggggttatg acaaaatttt gttataatcc tttttttgtg tcttaaaaaa gagagaaagt
    17521 ggttgaaatt acatttatcg tatataaaac aatccgtgta tagatgctgc attttgttca
    17581 ccaccatcaa aaaaataagg gactgtgaca atactattgt cacaatccct tattactgtg
    17641 tattctatta aatcaatcat ctttctcatc tgattcttta gttgcttgat tcgctcttgc
    17701 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcggtaccgc caacgccaaa
    17761 tttaaaggcg cttttcttcg gtccattttt cgcccatagc gattcaactg tttttcctgg
    17821 tgtttggatg gattttttat tgacagtaac ttttccaggt gtttgaccag taaatccaga
    17881 tacttttttc ttcatcacat cagatgataa ttcgaatttc tcatcagtgc caaaaatgtc
    17941 aggttttgct tggtaagctt gattgatcag attggcaaga taattagccg aacgtatcct
    18001 tgctttgctg tccattccag tattgtcatc gtgccctgcc cagctactga tggtaataga
    18061 cggagtagag acaattagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
    18121 ccaatctgct tttcctagat ttccgtttaa gcttgagatg gcatctttga atggtgtagt
    18181 gatttttgca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttccga
    18241 atagatccgg acagagttgc tttcgtgttt ataaattaca tttcctgcgt tatctgtgat
    18301 ggaatcgatg atatagcctt cttcatagac gccgccgttt gctaaagcct gaaatccgtt
    18361 ggtctgttga agcgtcgtga catctactgt acctaaagga gcagattcaa cgccccaatt
    18421 gtcatttgcc gggtagttca tctttgccaa gtagtgctcg taagcatatt gtttcgatcc
    18481 accggaattc aacacatctt gatataagtg ataagccggg atattgttgg accattctag
    18541 tgactcacgg acggtttgga aggtgttcga tccttcgtta gtagcattga cgattttctc
    18601 gccagcattt tctccttctt gccaagttgt tgcataatct gatacacggg attctgatcc
    18661 gataagcccc atgtcgattg caggtccata aacaagaact ggtttgattg atgagccagc
    18721 ttgacgttct gtatcgaagg catggttatt ttgattctga ctgtaattcc gtccgccaac
    18781 gaatccgtag atgcggccag ttctgttatc catcaataca tttccggttt ctacttgact
    18841 gccagcacca tcatctaaca tgtatccata attttcaacg ccattttgca atgcagcata
    18901 gacatcttta tcgattgtcg aatggattgt atatccttta ttttggatcg tttgctgtgc
    18961 cttttgataa taagcgtcac tgaccgatga atcagacata tcagcgttgt ctttttcagc
    19021 caattgttgt gcaatgattc ttgttgcttc attcatcaca gtataataca agaattctct
    19081 gtcagtctgt tctgcgattt gttgtggtag aaagtctttt gtaaggtcat aggcttttgc
    19141 ttcttcgtat tgttctttcg taatttggcc ttcacgatac atactgaata aaacaaaatc
    19201 ttttctggca aggccggcag aaagatcttc ttttagcgcg cctgtgttcg tgtatggact
    19261 atacgtgatc gggctttggg gtaatccagc gatataagca gcttgcggca gcgtcacatc
    19321 ttttgcatct acaccaaaga ttccttttgc agcttcctgg actccagcaa tgttttgtcc
    19381 cttattatta cgaccaaatg gtgagacatt caaatacgta gcaatgatct catcttttga
    19441 aaagtatttt tctacttgtg cagaaagaat gatctcgttt gctttacgtt tgaatgttgt
    19501 ttcatctgta aggatctgct gtttgactaa ttgctgtgtc aaagttgatc caccagaaga
    19561 gccaatacca gtagcttcag atactaatgc tcgtaaaaca gctttaggaa cgtaaccttg
    19621 gtgtttatcg aagtattcat cctctgtgct gataattgca gttttcaata aaggtgaaat
    19681 cttatcagat gaaatagtcg tgcgcatcag atctgtctgg atttttgata tctctgtatt
    19741 atctgcataa acaatctttg acgtttctgt aatgtttcct agttctgtct gcagtgcttt
    19801 tttagtcggc aattgtgtgt cttcgacgag ataggcaaaa tagccagcgc ctattccaat
    19861 acctagagct gcgaacatca aaagaagaac agtaataaaa acagtcaatg attgaaaaac
    19921 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
    19981 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
    20041 aaataataga acaaaaaaac agacaaggct gtttttggca ggaatttctt ttttaaaaga
    20101 gttaaaacgt gagaaaatga tctttattat aaaagagttc ttaagctttt ctttagagaa
    20161 aatttcagtt actatggaga acagggcaaa gaaaaatcaa tcattttctt tgtatatcat
    20221 cttaccaata tataggaatt gttagtaaca attgcctttc aaggaggaat ccatccatgt
    20281 tattaggctc acacgtcagc atgagcggaa agaaaatgct gttaggagca gcagcagaag
    20341 cagccagtta tggatcttcg acttttatga tctatacagg cgcccctcaa aacactagaa
    20401 gaaaagccat cgaagagatg aatattgatg ccggaaaagc gtacatggca gaaaacggtc
    20461 taacaaatat cgtcgttcat gccccttata tcattaattt ggggaatacg atcaaaccag
    20521 aaatgtttcc ttttgccgtc caatttctga gagaagagat tgttcgtgcg gaagctttag
    20581 gcgctactca aattacactt cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa
    20641 ttgctagaat tattgaaggc ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg
    20701 ctttggagac aatggcaggt aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
    20761 aaatcatcga tggagtaaaa ttaaatgaaa aattatccgt tacttttgac acctgtcata
    20821 cgaatgatgc cggttataat attaaagaag acttttcagg tgtaatggaa gagtttgata
    20881 agatcatcgg gttagatcgg cttaaagtac tccatatcaa tgattctaaa aatccgcaag
    20941 gttcgcataa agaccggcac gccaatattg gttttggaac aatcggattc gacacgctga
    21001 ataagatcgt ccatgatcca atgtttgaca atatttcgaa aatcttggaa actccctatg
    21061 tcggcaaaga taaaaagaaa gcttacgcac catatggaaa agaaatcgcc atgttcaaag
    21121 cacaaatatt cgatccagaa gtgtttcctg agttattagc agaagaagtg atactctaag
    21181 attttctatc tgacagcagc aaaaaaggac caggcacatt gccttgtcct tttttgttta
    21241 agaaacaaag gagtactttt tgcagtcttt ttcatcatgc atcttctccc ccctcttttt
    21301 aaatttagac aaacaaggta tactgttaga aaagtaaaaa aacgaaaagt ttcacaagca
    21361 agtggcaaaa cgtgattgga aggacgaaaa gatgctgaac actttaaaac ccttgatcaa
    21421 ccaactaaaa aagaaacata catactttat tcttggcata gccctattat ttacaattat
    21481 tcgtttgtat ttatatagta aagcaatcta tggaacagac atgtcggcaa cctatgatga
    21541 ccagctgctg attcgctatg ctcaaaatat tttggacggt aaatggctag gggagtatac
    21601 gactacaaca ctttccaaag gaatctccta cagcttattt ttagtacttg cacataaact
    21661 gcatctttct tatgctgttt tgttcggcat gttcagtatt tttgccagta tattggttgc
    21721 actcgcgttt aaaccgatcg tgaagaacaa gtatcttctt ttaagtatct atttttattt
    21781 tttgttttct ccgattagtt ttacacatga atattccact cgaacatatc gtaatgccct
    21841 cgtgattccg gctgttgaga tcattatcgc ttgtttacta gctatttatt accgcaaata
    21901 cggttctagc agaaaattgc tgccatggtt tttagggctt tctgtcgctt ttcctttctt
    21961 ttggttcatt cgcgaagatt ctatctggct tcttcctttt actgtagtag ctttgggtat
    22021 cagtttcgtc cagatcttgc tagggcaaga cttttctttc aaagaagtaa agaagttaaa
    22081 aaaacgactc tttcttctct cgaaacggga atggctgaaa ctttcattgt ttttacttcc
    22141 tatagttggc ttttccatcg tctatcagtg gatcgaatat aaaaatgaga caacatacgg
    22201 tattgcaacc atcaatgacc gttccagtgg agaatttgga cgtttgatga aacaactgat
    22261 ccgaatagat gatgaaacga acttgaataa agaaaacagt gagatatggg tctcacatgc
    22321 tgcactaaat aaagcattag ccgcatctcc tacattccat actgtttcta cccgtattga
    22381 cgaattatat acgactcacg cgtggactca aggtggaaaa gtaaaagaat tacccggaga
    22441 tatcatcttt tgggcattgc gagatgtggt aagcgaatct ggttattacc aaaacaatgc
    22501 aaaagaaacc aatgattttt ggaaaaaagt caaccaagaa ttagaagaag cctatcacaa
    22561 gggaaccatc aaaaagaaaa aagagttcta tctgatggga tcaggagatg gaaagacgat
    22621 gagtgatctg ccaatcgtat tcgatttcct tcaatcagga tacgcttatg catggcgata
    22681 taaagatttc catcaaggag gaaattatag tttcggaacg caagaacaag tcaaacaggc
    22741 tcaagaatta ttaaaccttc cgctaatgga gaattggatc gatagtaatg aaccgagaac
    22801 acctaatttc caattaacaa aatcagcaaa agtcgcgaat gtcatcataa aaatttacca
    22861 agttctggct attcccgtgc tgattgcttc aatagcaggg atattactta tgatactagg
    22921 aaccgtattt gcaaaagctg atcgtgccta ttatagttcc tctttgattg tggtcaccgg
    22981 attaatactg acacaattgg tttttctttt cggtgtctcg tggttttgtt cttatgcgcc
    23041 agaaaaaaag gattattttt taagtgtcta cactggcgca ggcgtgccat taacgcaatt
    23101 catcgtagta acaagcatgc tagttttcag taaattaaat ctgaaaaagg ctttatcttc
    23161 taaataaaaa gacccttggt agctagattt ttaacctaac tatcaagggt ctttgtgtgg
    23221 aaaaatcagt ttctattcga tttcttctac cgtttcctgc aaacgctgtt catatttttt
    23281 gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa gaacaatgat
    23341 tacataagcg atgtgatcag ccaatgctag ttgagcagaa gtggcttgtt caccaaagaa
    23401 actttcgatc tgtcctggta atcctcgtaa gttcaagtaa gttagactaa tcacagagaa
    23461 ccagccaaga agttttacga tgaatgaatt tttaaatcgg ctacccatct ccgcttggct
    23521 gttggtcatc atcagtaaag ggatcatcga aaacggcaac gcaaaagcca agaacacctg
    23581 ggattcattc atcaagttat tcaaagcggt atgttcttcg atcgttcctt ttgtacttgt
    23641 aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacca gtcttcttag
    23701 ccaaatcggg atacgcatat ggatgaagcc ttccatgatg acttgaccgg tcaacgtacc
    23761 agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag tggataagat
    23821 accagatttt gctacagcaa tcaagatacc attgctcata gctgatggat cagataaggc
    23881 ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaaga cagcgacacc
    23941 catgatcaat agcagtgcat tgactagaaa agccatcgac aattggatat tagagtccca
    24001 agtagaaaaa cgtacagctt gtgctacatc ttcttcgtct tggtgatcaa tcttccgagt
    24061 ttgagaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac caataatccc
    24121 taaagctcca gtcagcggag tttcgccgcc gatgaccggt gagtctgaga atgtatgact
    24181 gttagggata aagccagcaa ataaactgtg ccaatctggg ttagacaagc caacttgata
    24241 gacaaaaacg aataaaatga cgaaaattaa gcagacaacc agtgcttcga tcttacgaaa
    24301 cccgactttt gtcagtagta agagaacgaa aacatctaat acagtaataa agacggcaat
    24361 caccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatga cttctgcgat
    24421 gtctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccca atgtcttact
    24481 tgtgcgtgct cggattgctt gggccaaatc catttgggtc acgattccta attttgctgc
    24541 catatactgt aaaagcatag caatcagact agacatcaaa ataactgaca tcaataaata
    24601 ttggaaattt tgtccaccag tgatcgaagt ggaccagtta cctggatcca tataaccaac
    24661 tgctactaat gcacctggtc cagagtaagc aagaagtgtt ttaaaaaaac ttttcccctt
    24721 tggtacatca actgttccat ttacttcagc taaagataaa ccatttgtat gttcaataag
    24781 tcgatgctta tgaaattgtt tgttggattg attattcaag aaaaaaccac ctttcctatt
    24841 tatctacgat tacattctac acctcaaaaa gaaaaaatta aagaaaaata ttcggtatgc
    24901 ctaatttatt ttcgtggcgc gtcttgttag atgacgaaag aaattttgtt ctcctctaaa
    24961 aaaaccacta ttttttgcat gaaactaaag aatacgcctt tcttttttca ataaacttta
    25021 gtaaaataag tcattgagag caattcggaa atagaaagga gagatttata tgttatcaac
    25081 aggtattgtc gtacttattg ctattattgc tttgctaatc ggcgcagtag gtggcttttt
    25141 cctagcgcgt aaatatatga aagactattt agaaaaaaat cctcccgtta atgaggacat
    25201 gttgcgatca atgatgatgt caatgggaca aaaaccatct gagaagaaaa ttcgccagat
    25261 gatgcagcaa atgaagaacc aagggaaaaa ataggttctg acccaacgac tttttacggg
    25321 ctgatttttt cagtccgttt tttattttgt aatttgttag actattcaga ttattcagca
    25381 aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg
    25441 atttttagaa agttaggctg gttttttcgt caggaaaaga aacattattt aattggggtt
    25501 ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc
    25561 attgatgaga ttgccgataa aaatttatca gtaaagatca ttttcgggtg gatcttccta
    25621 ttgattatcg ctgctttctt acaatatctt tttcgttata tctggcggac gaatatttgg
    25681 ggaagtgcag ctcggttgga gaaagaactt cggcagcagt tattcaaaca ttttacaaaa
    25741 atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca tgcaacgaat
    25801 gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg
    25861 attacgggcg gaacaacgat cattgtgatg attttgttta ttgattggcg cttaacactg
    25921 attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcgaaactc
    25981 catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
    26041 agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat
    26101 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tctagactcc
    26161 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
    26221 ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt
    26281 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
    26341 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaaaac gcatatcgtg
    26401 gaacaactgg gtgcagtgca gaccccggta aaaggcgagc tgaatgtagc tatttcctct
    26461 tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc aatccaagaa
    26521 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacaacgat catcaaatta
    26581 ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa
    26641 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg
    26701 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
    26761 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
    26821 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
    26881 tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga tgcactttct
    26941 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
    27001 aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt
    27061 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc
    27121 ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag
    27181 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
    27241 aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaacaacg ttcatcgttg
    27301 ctatcttatt tgcgtttgct ctgtctgtga tcaatgtcct attgccaagg atcatccaaa
    27361 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
    27421 ctggtatgta cttatttgga gtcctagtaa aaagtatcat ttggtttttc caatggtatt
    27481 tgtattccat ggcttcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc
    27541 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag
    27601 tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg
    27661 ggatctttgc agtcgtttct tcattcattg ccatgtttca aatcaatgga gaaattgcct
    27721 tgtggacttt ggtgttcttg ccgattttag gaattgtcat ctggtattac cagaaattta
    27781 gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaataca aaattgaatg
    27841 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
    27901 aatttgaaca agtgaacaat gagtatttag acacgcgcta tgcgatgatc aaaacgaatt
    27961 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggcgttga
    28021 ctttatttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta
    28081 cgacttatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct
    28141 tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg
    28201 caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca
    28261 atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta
    28321 atccgggaga aaccgttgca ttagtgggac acaccggaag tgggaaaagc tcgatcatca
    28381 atgtgttgat gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca
    28441 ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt
    28501 tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg
    28561 aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa
    28621 aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc
    28681 tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa
    28741 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggcg aaaatgcgac
    28801 aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga
    28861 ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc
    28921 aaaatggttt gtatgcagat atgtacaaac tgcaaagtag tggtatataa aagatgttgt
    28981 gacagtagca ttttgaccta agcactagag tgaaaataaa acatagcttc ccatattttt
    29041 catctgtgag agttagtgtc gtagttacca gatttaaaaa agacgacgtc agctagtcag
    29101 aagtgcctaa tagcccctaa gaaagcgttg acaaaatcgt ttgctttcga taatataaag
    29161 aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat
    29221 ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc tatatatcat
    29281 ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataacaagg
    29341 gatgaaagag atcgggaaat gattgtgatg ggaaaaggat tggcattcaa aaagaaggtg
    29401 ggtgacccga ttcttgactc atgcatcgac aaaaaatttc agttgacaaa tgacggactt
    29461 tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt
    29521 gaaatcattg aatatacaaa agaggctctg caagtccatt taaatgattc tgtgtatatt
    29581 tcattgacgg atcacttgta tacagctatc gaacgtcaaa aaatggggat ctttgtgcca
    29641 aatgtattgc tgtatgatat caaacaactg tttcctaaag agtatgccat tgggaaaaaa
    29701 acagtgaaga aaatctcgga taaataccac atcaagttat cagaagacga agccggattt
    29761 atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg
    29821 tttaccacaa caatacagga tattttaaat atcgtccgct attactataa gattacattt
    29881 gatgaagatt cagtccactt ttttcgattc gtcacacacc tgcgtttttt tctgacacgt
    29941 gtcctagatg gagcgaaatc agaagatgga gaagtttctg aggaattgtt ccaagttatt
    30001 caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt tttaaccggg
    30061 gctttcgact accagatgtc cacggatgaa aaaatgtatt taacgatcca tactgcgcgt
    30121 ttagtgcatg agaatcgttg taaataaata aaaaaggagg acaaattaac aagaacgtat
    30181 gaaataaggg tagtgacatt cagttggcta aaccttccac taattaattg gagggaatag
    30241 aaatgggaaa ataccaggaa ttagcagaaa acatcgtaaa gaatatcgga ggaaaagaaa
    30301 atatccgtag cttgacacac tgtgtgacgc gacttcgttt caaactaaag gaggaaaata
    30361 aagcaaagga tgatgttttg aaaaagatgg aagggatagt caccatcatg aaaagcggcg
    30421 ggcaatatca agttgtaatc gggaatcatg tgccagatgt ctatgcagaa gtactgacag
    30481 tagccgggat ttccgatgaa gcagaagaag aggtttcttc aagtagtaat ctgttcaatc
    30541 gattaatcga catattaagt ggctgtttcc agcctttttt aggtgctttg gctgcagcgg
    30601 gaatggtcaa aggattaaat gctttgttct tgtttctcaa attttatact gaaggatcgg
    30661 gtacgtacat catgctgaat gggatagggg atgcaatttt ttatttcatg ccagtgatct
    30721 tagggtacac agcagcgaaa aaatttcatc tgaatccgat ggtggggctc gtgattggtg
    30781 cagcactttg ttatccgact gtacaaggag cagcactaca aagtggtttc cctgatggca
    30841 gtcctgcgcc ttatagcttg ctgggtcttc cggcttatga aacatttttg ggtatcccat
    30901 gggtgggcag cagttatact agtagcgtcg tacctgttat cttcattatc gcatttgccg
    30961 gacaagtcca aaaaattgcc aagaaatgga ttcctgcagt cgtccagacc tttttggttt
    31021 ctttttctgt gttattaatc gcacttcctg ctggcttttt gattatcggt ccgattgtca
    31081 gtatactgac agatctatta agcacgggat tccaagcatt gatggacttt tctccagctg
    31141 tgtatggtgc agttctaggg tttctttggc aagtactggt gattttcgga cttcattgga
    31201 gtattatccc tcttgcgatt atgcagctag cgcaaaatgg gtggagtcag atgttagtag
    31261 gcgtttatgg cgcgagtttt gcacagacag cagtggtatt ggcgatgtat ttcaagttca
    31321 aggatcaaaa aatgaaggcg ctttgcatgc cagctgtgat ttcaggaatc ttcggcgtaa
    31381 cagaaccggc tatttatgga atcaccttac cgaagaagaa gccatttatt tactcgatga
    31441 ttggtggtgc tgtttcagga atctatttga tggcatctgg aacaatcagc tacataatgg
    31501 gcggattagg gatttttggg gtggtaaact acatcacgga aacagacgcc agtggtatgt
    31561 ttcaagcgtt tatcggcatt gctattgctt cggtaatcgg gttttcgctg acttttttct
    31621 tttggaagga tgaagcaggt gaaacagatg ctatacagaa aaaatcaggt aaagaaattg
    31681 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcgaaggat gcagcttttg
    31741 cacaaggaac actaggtcga ggaatcttaa tctaccctga aaaaggagaa gtacgtgccc
    31801 cttttgacgg aacagttatg accctgttcc cgacgaaaca tgcaatcgga atggtttctg
    31861 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    31921 attttgaaag cttagtgcag caaggagaaa gagtgagaca aggcgatctg ttgctacgct
    31981 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    32041 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    32101 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    32161 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    32221 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    32281 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    32341 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    32401 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atcattttgt
    32461 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    32521 ccgcacatat cgctcgtaca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    32581 attgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    32641 ttcgagttac tggcgaagat cacgattttt tttgcctgga gcagtaaata tttagctttt
    32701 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    32761 gccattttat ttgcgattgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    32821 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    32881 acccagccat caaaacccat tttttttgct acacggatga gtgtagacgg gtggacataa
    32941 catcgctcag ctatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    33001 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    33061 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgttt
    33121 tgtaaacg
//
LOCUS       DAXWLB010000009        32507 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXWLB010000009 DAXWLB010000000
VERSION     DAXWLB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.009"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            64..741
                     /locus_tag="ACGE4H_000358"
     CDS             64..741
                     /locus_tag="ACGE4H_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8363802.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            744..1871
                     /locus_tag="ACGE4H_000359"
     CDS             744..1871
                     /locus_tag="ACGE4H_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363803.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2071..2511)
                     /locus_tag="ACGE4H_000360"
     CDS             complement(2071..2511)
                     /locus_tag="ACGE4H_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8363804.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(2522..4555)
                     /locus_tag="ACGE4H_000361"
     CDS             complement(2522..4555)
                     /locus_tag="ACGE4H_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFF8363805.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4557..6260)
                     /locus_tag="ACGE4H_000362"
     CDS             complement(4557..6260)
                     /locus_tag="ACGE4H_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8363806.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6354..7304)
                     /gene="manA"
                     /locus_tag="ACGE4H_000363"
     CDS             complement(6354..7304)
                     /gene="manA"
                     /locus_tag="ACGE4H_000363"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFF8363807.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(7343..8368)
                     /locus_tag="ACGE4H_000364"
     CDS             complement(7343..8368)
                     /locus_tag="ACGE4H_000364"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8363808.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8577..9446)
                     /locus_tag="ACGE4H_000365"
     CDS             complement(8577..9446)
                     /locus_tag="ACGE4H_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8363809.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(10150..12042)
                     /locus_tag="ACGE4H_000366"
     CDS             complement(10150..12042)
                     /locus_tag="ACGE4H_000366"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363810.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(12039..13766)
                     /locus_tag="ACGE4H_000367"
     CDS             complement(12039..13766)
                     /locus_tag="ACGE4H_000367"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363811.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13782..14432)
                     /locus_tag="ACGE4H_000368"
     CDS             complement(13782..14432)
                     /locus_tag="ACGE4H_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFF8363812.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14636..14932
                     /locus_tag="ACGE4H_000369"
     CDS             14636..14932
                     /locus_tag="ACGE4H_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8363813.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            15124..15744
                     /locus_tag="ACGE4H_000370"
     CDS             15124..15744
                     /locus_tag="ACGE4H_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8363814.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15836..17002)
                     /locus_tag="ACGE4H_000371"
     CDS             complement(15836..17002)
                     /locus_tag="ACGE4H_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8363815.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(17014..17367)
                     /locus_tag="ACGE4H_000372"
     CDS             complement(17014..17367)
                     /locus_tag="ACGE4H_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8363816.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17495..18517
                     /locus_tag="ACGE4H_000373"
     CDS             17495..18517
                     /locus_tag="ACGE4H_000373"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFF8363817.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18536..18697
                     /locus_tag="ACGE4H_000374"
     CDS             18536..18697
                     /locus_tag="ACGE4H_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363818.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18956..19390)
                     /locus_tag="ACGE4H_000375"
     CDS             complement(18956..19390)
                     /locus_tag="ACGE4H_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363819.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19500..21359)
                     /locus_tag="ACGE4H_000376"
     CDS             complement(19500..21359)
                     /locus_tag="ACGE4H_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8363820.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMVLFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            21360..21500
                     /locus_tag="ACGE4H_000377"
     CDS             21360..21500
                     /locus_tag="ACGE4H_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFF8363821.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(21477..22636)
                     /locus_tag="ACGE4H_000378"
                     /pseudo
     CDS             complement(21477..22636)
                     /locus_tag="ACGE4H_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(22943..24367)
                     /gene="araA"
                     /locus_tag="ACGE4H_000379"
     CDS             complement(22943..24367)
                     /gene="araA"
                     /locus_tag="ACGE4H_000379"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFF8363822.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24383..25081)
                     /locus_tag="ACGE4H_000380"
     CDS             complement(24383..25081)
                     /locus_tag="ACGE4H_000380"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8363823.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(25038..26699)
                     /locus_tag="ACGE4H_000381"
     CDS             complement(25038..26699)
                     /locus_tag="ACGE4H_000381"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFF8363824.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26917..28317
                     /locus_tag="ACGE4H_000382"
     CDS             26917..28317
                     /locus_tag="ACGE4H_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFF8363825.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28569..29648)
                     /locus_tag="ACGE4H_000383"
     CDS             complement(28569..29648)
                     /locus_tag="ACGE4H_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8363826.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29734..30693)
                     /locus_tag="ACGE4H_000384"
     CDS             complement(29734..30693)
                     /locus_tag="ACGE4H_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFF8363827.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30705..32216)
                     /locus_tag="ACGE4H_000385"
     CDS             complement(30705..32216)
                     /locus_tag="ACGE4H_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFF8363828.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(32238..>32507)
                     /locus_tag="ACGE4H_000386"
     CDS             complement(32238..>32507)
                     /locus_tag="ACGE4H_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363829.1"
                     /translation="ISGYVVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDF
                     LRNILCCVLLLGFVLLIFLFPPFIFIFAGGVWKLVSIILTIGNER"
BASE COUNT         9261 a         6787 c         5725 g        10734 t
ORIGIN      
        1 agcatttaat ttagatgttt tccattctat tatgttatta aattttatat tggaggccta
       61 tctatgacat tactgatcaa aaacatgaac atgaaacgtg gtaaaaaacg tgtgattcga
      121 caattgaatc ttttagtaga atcaggagaa gtagctgctt tgattgcccc gaacggattt
      181 ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc caacaactta tgatcaattg
      241 tcccttcaca acatctctcc tactgcttat acagattatc gccggtcttt cttttttcta
      301 gaatcaacga atcagttatc cgactatcta tcggctgttg attatttgac tttgatcaaa
      361 tactattggc agagcaaagt agaatgccaa actgttttcc aacgattgaa gatgactgag
      421 taccttactt tgccgattgg aaaaatgtct ctaggaatga aacaacatgt cttgcttgct
      481 gcttacttga tcagtgattc atctgtcatg ttattcgatg aaccgttgaa cggattagac
      541 ccaggaagta ttgatatctt gaatagcctc atcagacaat ggcagaaaga ggggaagacg
      601 attcttcttt cttcccacgc tatcgcgaat atccaatcga tttgttctaa agcatgtttt
      661 ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg aagagatttt agctatttat
      721 cgtaaattat atgaggtgta actatgtctt tacttacatt tgaacgcaat ctcttactaa
      781 aaaacaaagt aaacttttta tttcctctat tgttggtcgt gctctttgca ttcccacttt
      841 tttttgatca taaacttgct tatacagaat tcgatgaact gaatcataac tatgaagaga
      901 tgcaacgttt gattgaaacg ttgaaagaag atgagaatga gaaagaattt gtggaaagtt
      961 tagagaaaag caacaaactg atcgaagcaa tcttacatgc aaaaaacaca gggaatgtcc
     1021 agcaaatggt cgaagcaact tatcattatg aaaaagatat actggatcga ctgatttctg
     1081 gtcaaagaca aggaattcca atcattgaac aacaaaaacg tgtcgagttg ctccgttata
     1141 tgaaagaaca tcagatccaa agatattcta tttttgactt gccagcgcat ctctctttag
     1201 caaattacta cgaaaatatt ttttcaggaa tgatttctag ttttttgatt ttatgtataa
     1261 ccgccttgtt tctttcttcc atcatctctt atgaaaaaag aaagcaggta atctctctag
     1321 tcaatctatt accggatagt atggtaaaga aacatagcat ccgttttacc atttattatg
     1381 gtgcagctat gctaagtctc gtgatgccat ttttgatcgt ctctatacta gtaataatca
     1441 aaaatggttt gggagacttc cgttatcctg taggaacgat cattggtcag gagatacgaa
     1501 tcctgccgat gtatgagtat cttttccaga gctttctgtt cctgttgttg tgggtgctct
     1561 ttttaagtac aatcagtttt cttctatctg ctttatttga acattctcta gtcaatcttt
     1621 taggtacact tctttgtctt ttcttagcag agtatcgctt gttttcttcg attggatgga
     1681 tagaatcaat cagtcattac ttgccaactt cttatgttga ttttcaaaat gtgatcatag
     1741 gaggagatat tttctcgcca ctagcttcag aacaggttac tttcatgaat ggtatcttga
     1801 cacttggtat atggagtatc gttttattat tcataggtat gggcactatc tatataaaaa
     1861 agagctatta aaacacaaaa ataatagggg cggtgacaaa aaccttgtca ccgtccccta
     1921 tttcaaataa acggttttct agaataaata gttttcaaaa gcttgcatcc gcagtaataa
     1981 gaacctccca acaaaaatag ctcctcttat ttttatcttg gttgaagtta ttacttgatg
     2041 caaggcagct ttttttactg tgaacattgg ttatatatgt ccatgttcaa aaatcatttg
     2101 gacttcttca atggtttccg catcactgag cgccattact tggtcgatgt ctgaacagaa
     2161 aaaggctaaa tcttggagca ccgctaactg atgttctttt tttaaagcca ctacaaataa
     2221 gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc acaccatcag gcacttggac
     2281 taaatagatt ccttcttcta ctacttcttc atctttgtca gtcccatgtg gcaaggctac
     2341 gaaattcccg atatagacac tcgctttttc ttgtctttcg atcattgatt ggatatatgc
     2401 cggtttgatt cccccttgtt gggtcatcca ctggccgatc tcggtcaacg cttctttcca
     2461 attttgatag gattgatcga gcaatatttt ttctgctggt agctgtatca tcttttcacc
     2521 ttcatttctt tatttcttca atcaggattt tcgctaacaa ctgataaacg atttctttat
     2581 ttcctgagcg gaagatctcg gtgttcaagt cattcatgat gattgctcca ctgatctttc
     2641 ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc cagcataact aacatacggg
     2701 acaaatcgac tggctgtcta tccatccctt caatggtcaa cggatgtttc aaattgaaaa
     2761 tacaaaagat cggtttagaa atcaatgagc tggatgtatg gaataacccc atatttgtat
     2821 gaggaatacc gataggtgct tgttcatatc gcttaagcag ccggcgatga accacctctt
     2881 catccgttac tagtccttca ggaaaataag gaaataagct gcgaatcgtt tcggaaatcg
     2941 tctcaggatt atccagttct tggataaaaa aacgaccgag taattgattg atttgatcca
     3001 tgaaatgcat tgtttcctca taagtatctt ctatcatcca ctcgttattg ctgttatacg
     3061 taattttttg cctttttttc ttctcaatat gcgaaaaagc ttcttttagc tgcttgactt
     3121 catcttctag cagtagcggg gagactaaaa tatattttcc accgtatcct ggtaaaatag
     3181 atgtagaaat aacaacatca tattcttctt gtaaatcgat tttttttaaa tcagcaatcc
     3241 gatagaaatg aatttgttgg atgaagggaa aatacctttt caatcgtagt tccaacatgc
     3301 tagtagaagc taatccactt ggcgaaaatc ctgcaatact gatattgctt tctttcggac
     3361 ttcgttccaa tgaattcgca aaatgaagga ccatataagc gatctcttct tcggataatt
     3421 ttttttcttg aaaaatccgc tctactgctt tacggattgc attcgcaact tcctcgtatt
     3481 gaaccatgat tttttctaaa atcggattgg tcagtgtctc ttcttgtaaa acggctcttg
     3541 aaaacacacc agataaatga gtcaacagca ttttgtacaa agtttcatct tcataaaaat
     3601 ggacattcgt ctcttcactt acttcttgaa tcagttgttt tactaaataa gcaagctgtg
     3661 tatcgaacgt ctcttcaaag aaattctgat caaatgagtt cgtgaagtca tttaataatc
     3721 ctgcaaaaaa gacgatctca tttactggat aaagttgttt tgtctcagaa gcgatccgtg
     3781 aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc cagactatta tatgtctcat
     3841 ttgtcagtaa atgatttttc gtcacacgct ccatcgtcaa tgtcagcatc aaaaccaaat
     3901 gttcaagctt tctatcactt agattggtct ttttttggat caattcttcc ccgattactt
     3961 tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa aaaataattc agtgatgcat
     4021 cgaaagaaat actatgaaaa agttcatatt cattgatttc taattctaat acattcgctg
     4081 tcagaagccg gcggtatttt tccggtcctt cgatctcgta tccctggttc cgaatgattt
     4141 ccaaaggcag ttggcgaata ctttcttcca actgtttgat atctgcataa aaagtggtat
     4201 tactgatgcg atatgattcc atgaaatgag caagactcaa cggctctgtc gtcagtaaca
     4261 actcggctaa aatcgcgtgt tgtcgtttag cagtggataa gtcattactt ttcccatctg
     4321 tattttcttt taattgctct aatacttctt ctgatgcctc aatgacaaat gagcctctag
     4381 ctgctttttt caaagaagca tttaccgatt tcaatgagac ttcaagatta tctaattcgc
     4441 ggtaaatagt ccgtttactg accttcaata aattcatcat tttttggata gatacaggtg
     4501 acgggctact gagaaattct gctaataatt gtttttctct gggcgataaa tacatcttat
     4561 atttctccct tcacgagttt ttcgtattca ttcgtagcta cccaatgttc aactagaact
     4621 agccgaggga tcttttcttc tagctgttca gttacagcta gttctctttg tgcgatataa
     4681 atcgtttctt cttggatttt gattttttct attgattctt ttgagataac aaactgtttt
     4741 ttcgcttttt ttgctaattg acttaaaatc gtcatgccca tagtttgtga gccgcgtctg
     4801 ttgtttttat acaaaaatac aatattagca ggcgacgttt tttgttctgt gatatctgct
     4861 atctcttttt tttcttcccg ctttggaaat tggatcgctt cttttattcg tgacagcaag
     4921 atatctgttt ccaaaaagtg atccagtaca tagatttgac tttgaggtac tttttctctt
     4981 gcaagacccg ctagttcatg ttgcacgata accagctgat ttacttggtt attcaattca
     5041 tagatagagc tgtatccgac agaaagatcg attttttctg ctgctaattt ctttttcagc
     5101 agggcggctc ccatagcact tgatcccata cctgagtcgc aagcaaaaat gacggaatga
     5161 atgatagatg gttccaagat ttctcccctt tctttgaccg ttttgttctc tttttcttta
     5221 tttggtaatt tcaatagtat cgctgcacaa atgaaggtca caaatgtact gattgctatc
     5281 cctatactta ccccagctag ttgtggataa ggcgcattgg caaaaatcac gagtagtgac
     5341 ccaggcgata caggtgcagc taatcccgta tctaaccatt gaaatatcaa cgtgccgctt
     5401 gccccaccag ccattacagc taaaaataga tacggtcgca gcaagacaaa tggaaaatag
     5461 atttcgtgaa tcccgccaat cgcatggatc atcaatgctc cactcgcatt tactttttcc
     5521 tgtttttctg caaaacaaag gatagaaagt aagatcccta aaccaggtcc aggatttgcc
     5581 tccatcaaaa ataaggaaga gtacccgact gtttgtgaga attcgattcc taaaggtgtc
     5641 aatacaccat gattgattgc attattgaaa aatagtactt ttagaggttc taagatcaga
     5701 ctgacaagtg gtaacagatt cattgtgact agccaagaca caacggaggc gagcaacaag
     5761 tgaacactat ttaaaactgg ttcaataaaa acgattccaa ttatacaaat aacgctacca
     5821 gtcagtccta ctagtaggtt gctgacaagc atttcatatc cagccttgaa tttcacatgg
     5881 aatctagaaa caaattgttc atatacgatg ccggcaagcg gcccgataat catcgctcct
     5941 acgatttgtg gagcattgga acgagcgatc aaccctaaag aagcaattgc accggcgact
     6001 gccccttttt gtccccctac cgatttcccg cctgaataac tgatcaagat gggtaaaagc
     6061 agctggatca tccaatggca tacctcttct aatgtggtgt gcaaatcgcc agacacacct
     6121 tttgttgcta atgacaagat tccccaagcg ataaacagac ttagatttgg catgatcatt
     6181 tcatttaagt gatgtccagc ttttctcagt tgtttcatag acacgcgact gctgttgttt
     6241 gctttccatt tgtattccat ctttttcacc agacttctat ttattgatgg acggatttta
     6301 tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc agctatctgg gaactatttg
     6361 cctggttcag acgcaatgat tgctagttca ccttggaccg cccactcaga aacgccattt
     6421 ggaataatac agcttgttcc tttttctaat ggatatgttt ttccgtcaac gacaaggtct
     6481 cctgctcctt cgattaccgt catcaatgta taaggggctt gttttttgaa agatgtgatt
     6541 cctttaatat cccactcata tacattgaaa aactcagttt ctaaataagt gacgatcgat
     6601 gaattccctt ttcggacttc tttgatttgt aattctggtg ttttcgcagg gacagttgtt
     6661 acatcgatcg attgttggat atgcagctct cttgtttgtc cttgcgcatc ttttctgtcg
     6721 tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg tttctaaaat catgattcct
     6781 ttaccaatag catggatcgt tccgcttggt acataaaaga aatcaccttt tttaactggt
     6841 actttcttca aaaggtcatc ccagcgccca tctttgatca tctcagctag ttcttctctt
     6901 gtctttgcat gatgaccata gatgatttct gcaccaggtt cagcatcgat gatataccag
     6961 cattcagttt ttcctaattc accttcatgt ttcatcccat aggcatcatc tggatgtact
     7021 tgtacagata gatcatcttc tgcgtctaag atcttgatca ataatggaaa cactggttct
     7081 gaaggatggc caaacaattc cttgtgttct gaccatagtt catctaatct tttccctttg
     7141 aattcgccat tttctactac actgacacca tgaggatgcg cactgatcgc ccaatcttct
     7201 ccgattttat cgttagggat atcaaaacca aagactgaac gtaaacgact tcctccccaa
     7261 attttttctt gaaaaactgg ttttaaaaac ataggttcct gcataattca aactcctctc
     7321 atttttcata gctattatag cattatttcg gtaataccgc tttctctttc aaaaagtttt
     7381 caacaatttc atctcgacat atgagatact ccgcacgatg atcgacagga tgtacgcgat
     7441 ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat caagaaaaac gtacctgtat
     7501 aaccagtatg aaaaagaatc gcatgaccgt cttctacatc cttttttaag tcccatccta
     7561 aagaacgata gccatttttt ttcggtgttt gatcttgcag taaagaatca atcgtctctt
     7621 cttctaaaaa acgaccatta cggatttttc ccttgttcaa atacatttgg acaaaagtaa
     7681 tgctgtcttt caaatcaata aagagacccg cgttacctgc atgttcagcc aaaactcttg
     7741 ctttcggatc atgagtttgt ccacgcaaaa cctcacccga aggcagttgt tctgtgggaa
     7801 cgattttttt ggaaggaaaa ggaaggaata agctgttcat cattcctaat ggttccaata
     7861 cttgttcttg aaagatttcc actgcagttt cttggtaaat ttcctctagc atcaacccaa
     7921 gaagaatcgt accggcatct gtatatttta cttcttttcc tagttgctca cctggttcta
     7981 gcttcaaata agcggcgatc aattcttcct gagatagctt atctctgttt ggtatccatg
     8041 ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt gattgtctga ttttcaaatt
     8101 caggaagata tttatgaagc ggttgatcga gttctatctt tccatcttct aatagacgta
     8161 aaactaatga agtggttgca acgactttcg tcagtgaggc tgcatcaaac aacagatctt
     8221 ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc ggctgtaaat gtttctactt
     8281 gattttcttt tatgaaggcg actgctacac ctggataaaa tccttcttcc atctttttca
     8341 aaataagctg tttcgtgtgt ggatacatct ttttctcctg cttttctatc agattcttac
     8401 agtccaagct taaagcttaa tctatgagga atcaaccttt tttacacaaa aaaactgcgc
     8461 gaaaaaccgc gcagtttccc ccggtatact attttctctt tctatctcta gaataagcga
     8521 tagtatgacc gttcataaaa tgacgagaca tgcgacaatc gttaagaaag aaaaagttaa
     8581 gcgtcgcatc gaatgtcagt tttattcttt tcttttggct cactgacgaa ccaccattcg
     8641 attcctatag catcatagat tgtgagtact gtgtgctttt tgtctaagaa gaaatcaact
     8701 gatagttgct gcaagtgagc aatcagtcct tccatgcttt cagtatcgat catgaatttc
     8761 agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc gctagcttct
     8821 gtcaaaccaa catggaaatc tccttcattc aatacatgga tgccctcttc ttcattatgt
     8881 accttcaaac caagtacatg attaagaaat tggcgttctt ttgttaagtt agtaacattt
     8941 aaatggattt tttcaaaata agaacctgtt gatagtttac tgaatttttc ttcagatttt
     9001 cttaataatg cttcttgatc cattgtttct ggaatttctc tttggtcgtt cttattctct
     9061 tcatggtaaa tttccaattc attctcttct gggtctacga gcaaaatccc catacgtcca
     9121 tgatcttcta aagcttcttt gattggataa ttcattttac ggatacgaca aaggatatct
     9181 gccatttctt ctgtgctagg gataatcaat gaaaaacggt tcagcttttt gatcaccccc
     9241 atgtgatcat tcgctctagg actttcttcc agcagcaaca tttcacttcc agcttcttct
     9301 gttcctaaaa tggctaattc gttttcttct cttttcaaat caaagccaac aacgttcttg
     9361 taaaaatcaa tcattttatc tcggtctttc acccgaatgg ctacactttt taaataggtg
     9421 gcagccccca attgaaaatc tttcatattt cttgctcccc tctacgtcaa gctttatttt
     9481 aacgatggat tcttcttctt tcaagcaata actttgattt tgttaaaaaa aatgtgagaa
     9541 aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa aactcttata aaaagaggtt
     9601 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat
     9661 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga
     9721 ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttct
     9781 acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttaccaa
     9841 gaggttgtga gcctgccgtt taactccgag tcacaaggag caatttcaaa aaacagcttc
     9901 tcaggtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttaag
     9961 gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac ggaaaaatag
    10021 cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg aacaccgttt
    10081 attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa ataataaatt
    10141 tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat ctgcatagaa
    10201 tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac catgattcat
    10261 cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa cgaaacttgt
    10321 ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc gtgtatctac
    10381 actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg cccgcgcaat
    10441 tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta atacagtgtt
    10501 atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag cagcctcaat
    10561 gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga tacttcctgt
    10621 aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat cacgtgaaag
    10681 atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt agaaccgttc
    10741 aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag cgaccatctc
    10801 accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg tgtagccgaa
    10861 ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa ggccagattt
    10921 ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg aagcaactgt
    10981 tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga actgtgttgt
    11041 atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa cttttactcc
    11101 gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca acggcatgat
    11161 gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat tttctttttc
    11221 aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat gaccaccata
    11281 tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg cgaaaaacac
    11341 ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca tcaataacgc
    11401 gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa tcaatgtgac
    11461 tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt tatccatatc
    11521 attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg aaatcggtac
    11581 acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt gggaaacacg
    11641 tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca tcacgattac
    11701 tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta cttgttggcc
    11761 ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg ttgctttccc
    11821 caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta caaatacagc
    11881 gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg ttccccagaa
    11941 atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat gtcctggacc
    12001 gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc ctcctctctt
    12061 agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc ttcatgtgtt
    12121 cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc cattactgta
    12181 cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt caatgcttgg
    12241 cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa cacatagata
    12301 tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc accagagaag
    12361 tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga aacaaaatct
    12421 ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc cggcttacca
    12481 tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg aacaaagccc
    12541 attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt tatttggatc
    12601 gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt tttacctgaa
    12661 ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc aatatcttcc
    12721 aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc caatgttgcg
    12781 ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc gattttttca
    12841 tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat gataaaaata
    12901 gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa tgcaatcaga
    12961 ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt gattgctacg
    13021 tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat cgtattaact
    13081 ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc ttcaaagcgt
    13141 gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa taccaggttt
    13201 aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta catgattccg
    13261 ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa ctgatgatct
    13321 ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat catcatacgt
    13381 aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct tgtgatcaaa
    13441 gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt tttatagaca
    13501 tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa tgtatttgcg
    13561 atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt atgccaaata
    13621 taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag tgtcggcaaa
    13681 tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc tgaagtcaag
    13741 gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata tactccgttt
    13801 ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga gtaatcttca
    13861 gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt catcactaat
    13921 tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg atcgatcaat
    13981 tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg cgttttttgg
    14041 cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat aaacaagtta
    14101 cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga aaaataggat
    14161 tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat atctttagag
    14221 ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc ttcaaaatac
    14281 tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat cgaagcttct
    14341 tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt ttgtttttct
    14401 ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc tcctttcttt caagatgaca
    14461 cattgtcata tgacgctatg tcattataac acctcctacc tgttttgcaa gacatttgca
    14521 tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc cttccatttg
    14581 aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga gataaatggc
    14641 ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca gcatcgtttt
    14701 agatacaaga aataccttag attacacaaa aaaccactta agccactcgc tttctatccc
    14761 cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt attatattct
    14821 aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta atggttttcg
    14881 tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt gaatattgct
    14941 tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt ataccctgtc
    15001 tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt ttctttttcg
    15061 agtaaactga ttttgttagt catagttata acaatcatag caataaaagg agaaggtaag
    15121 catatggcat ctgttttagt agtcaaaggt catcccctaa ctgcagaaga atcgcgcaca
    15181 gttaaggcat tgacgtcttt tttaacaagt tataaagaaa atcatccaga tgatgaagtg
    15241 actgttttgg aactttatcg cgatgatatt ccggaaatcg atgaagaatt attatcaggt
    15301 tgggaagcat tacgtgctgg tgcagaattc acttccttat cagaaagtca acaacaaaaa
    15361 attgctcgtt tcaatgaatt gactgatcag ttccttgctg ctgataaaat cgttatcgca
    15421 aacgccttat ggaatctaaa tatcccaaca aaattaaaag cttggttcga taccgtcaat
    15481 gtagctggaa aaacgttccg ctatacagaa aacgggccag aaggattagt gacaggtaaa
    15541 aaagcactgc acatccaatc taatggcggt gtatacaacg gacaagactt tgcctctcaa
    15601 tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag tagatcagct attcatcgaa
    15661 ggaatcgatt atgaccctga ccgtgcagat gagttgatgc aaaatgcatt agataaagct
    15721 gctgctttag gtaaatcatt ttaaaaattg agataaatgc atacaaaaga gacagaggaa
    15781 aaaatcttca aagcagcatt tgtgccttgg atattttccc atctgtctct ttttgttatt
    15841 ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg taccattcaa
    15901 aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag atcacaccga
    15961 tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact ggatggattg
    16021 ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc tgctctactg
    16081 caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct aaaaagatag
    16141 ccggaatcat cgctaaaaat gatcctaagt aagggatcag gtttaaaaaa cccgcaataa
    16201 tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata aacatgattg
    16261 ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga ctattcattt
    16321 cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg acaaaataag
    16381 gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc gtcagcagag
    16441 ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact gtactcttag
    16501 atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct gtttccaact
    16561 gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta ttatcaatcg
    16621 tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga atcttaggga
    16681 tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc ccaatgatac
    16741 tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc atcaagtaat
    16801 acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc cagattggtg
    16861 caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac aatacaacaa
    16921 gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat accggtctgt
    16981 tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt ggcttcccaa
    17041 accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt gtccttcttc
    17101 tggacaagca tcaaaaatca atgatacgtg agctactgtc tttaaattcc cattggccat
    17161 cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc cgcctgcttt
    17221 caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc gtaaggcagc
    17281 tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg cttctgtccc
    17341 aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt tcaaaaacag
    17401 tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat aaaaacgaaa
    17461 actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa ttttaggaac
    17521 ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag aaaaagaaga
    17581 attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag caaaaagtaa
    17641 agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg gtgcgtacga
    17701 taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt gttatgtagc
    17761 ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg aagtaaatgt
    17821 ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc atcaagaagc
    17881 gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc tgactcctga
    17941 ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg atgtgtccga
    18001 aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag gccctcgcca
    18061 tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag acagcacagt
    18121 ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga aaatatcgac
    18181 cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt caaatgatgg
    18241 caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg cagtttttga
    18301 agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt ttccccgcga
    18361 tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca ccgataatct
    18421 gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa aagatgtcta
    18481 tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga atgaaatgaa
    18541 tcagcaagac gtattagaca gactacggga agaactaaat attcctttct ttgatgggaa
    18601 aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg atttacagaa
    18661 ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata ctatctgaaa
    18721 agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa ataaaaaacc
    18781 gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt aagtacaagg
    18841 ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct atcataaagg
    18901 taatattaca tttatattag ataactctag tttttattgt tatcaaagat ttcttttatt
    18961 ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct gtcgcttggt
    19021 cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca agatcgatac
    19081 tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca aaatttttat
    19141 gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcataagc attgcccgat
    19201 atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat ttgtccagtt
    19261 cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga tgcattcctt
    19321 ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa tagccaatat
    19381 tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt ctacttgttt
    19441 ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc tcgccattct
    19501 tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac aatgtcatat
    19561 tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg cgcttcttta
    19621 taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc ctgttctaat
    19681 gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat tagattcgct
    19741 gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa ttctccttta
    19801 ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt tgcttggatc
    19861 gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt ccttctagaa
    19921 agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga aatatcttgc
    19981 agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg atagtctaga
    20041 ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg aatgaactga
    20101 ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc atcagaaata
    20161 tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat gaactggtca
    20221 aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta tggatggatc
    20281 gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag ctgatccatt
    20341 tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacac catagaaaaa
    20401 ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg agaaagcata
    20461 ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc accagatact
    20521 tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa cacgatgttc
    20581 ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta tttttcagtg
    20641 atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc taccttcaga
    20701 ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg gctgtaaggt
    20761 atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt gttgaaatta
    20821 ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat caattggaat
    20881 cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat cacggttcct
    20941 gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag caatccgatt
    21001 tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact cattttcata
    21061 taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc taaagggaca
    21121 gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca ggctaagaag
    21181 aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga tagatcattt
    21241 tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact gatgattgct
    21301 gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata tcttttcaaa
    21361 tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt agttgtacga
    21421 aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct ctttctttat
    21481 ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt ttctagctgt
    21541 tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa tatgtagaca
    21601 tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc attcagcaga
    21661 gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa tcgaaacggc
    21721 gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt accgaacaaa
    21781 agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc ttcctgagtc
    21841 gatcccctag atgaggcaac ataagtatac ggtcattttc taagaactct tcttctggaa
    21901 aatcagtcaa gtatttagcc agatgttgtt tttccaatgc ttgcataaca gcatatttat
    21961 caacaatttc acttcttcct acattgataa gtacagcatc ttttttcatt ttttctatat
    22021 tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg cagcatgact acgatgtaat
    22081 ccgaggtaga aagaactgtt tctaaatctg cttgccgaac ataatccaac tgggcatcgc
    22141 ggatagaata acccaagaca tccatcccta agctatagca acttagcgct acttttgtgc
    22201 ctatcgctcc aagaccaaga agaccgatgg ttttgccttg caattctcgt cccacataag
    22261 cacttctttt gttttctgcc tgttctaaaa tattaggacc tgttagtgtt tgtaccatac
    22321 gacttgcttc gattatcgga cgggaagata agagcaaaca gcacaaaaca agctctttta
    22381 ctgcattagc atttacacct ggtgtattca tgacgatcgt cccgttttca gaagcttttt
    22441 caacgttgat cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa tctggtgtaa
    22501 gccattcatc tttcactttc gtactacgaa taactattcc ttcggctggc aaatcatcta
    22561 ttattttgaa atattcccct tgtaactttt ctatctcttg ttctggaata gcatcaatca
    22621 aatgaacgtg atacataaaa ccccctcctt actaacaagt atctcgaaat ttctgcaatt
    22681 agacaactgt ttaccatttg acttcctaga agcaaaaaga gcagttagag cagagtaggc
    22741 tgagtgataa aaataaggct atgacaagtt actgccataa gccttatttt cgagagcaaa
    22801 atattgaggg tgagatatct tgataaatgg tgttccgtta gctgacattg ataataagac
    22861 taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc ttaaaactaa
    22921 gtggcttttt cacaacctct ttttatctaa taaccactgt ttccaactca actaattttg
    22981 cccacgtttc aatttgatct gttgttagat ttagcgagac aacggtatga tgaccgccac
    23041 ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg tttagttttc cataacacac
    23101 gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa ggcttctact tcattgatca
    23161 gcagtttata atgcgtaccg aaatctgcca tcgatacgac taccccttct cctgctttcc
    23221 catcaaatac aagacgcgcc ggatcttctc tatctcccat tgataaagga gaaacaacga
    23281 ttcttggttt cgtcgcagca agcgtcggat ctacttccat catatgggat tccaagattg
    23341 cttcttggcc actagctagc tcgtaagtat aatcttccat gaatcctgta tctttccctc
    23401 gagccatgat tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct tccccagcaa
    23461 aaccgtatcc ttctgccatc aaacgttgta cagctagtcc aggtagttgt ttcattccat
    23521 acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag aaacggcgaa
    23581 taccgatttc ctgctgagct tgtactttta catgttcctc ccatgtcttt tcttcataat
    23641 caccatagtc gaaggtatac aattccttgt actcttcaaa taattgatcg acttcttcgt
    23701 ctgatacgcg atcgattact tgtacaagat ctccgatacc aaaataatca acagtccaac
    23761 caaattgaat ctgtgcttcc actttgtctc cttctgtgac acccacgttt ctcatgttgt
    23821 caccaaaacg agccactttt attccaaaac tttcattata ggctactgca acatccatcc
    23881 aatcagcaat ctctttttgg atctcaggtc gtttccagta gccaacgacg attttatttt
    23941 gtttatttaa tcgagcattg atgaacccat attcgcggtc accatgagcg gattgattta
    24001 aattcatgaa atccatatcg atcgttttcc aaggaatgct ttcgttatat tgcgtagcta
    24061 aatgaagcaa tggtttttgt agaagctttg tcccgcggat ccacattttt gctggtgaga
    24121 aagtatgcat ccaagtgatc acacctgcta cttcttctcg ataattgacc tctttcatga
    24181 tttttgtgat cgtatcagca gtaactgcta attcttgaag gcggattgga taaggcaatt
    24241 ggccattttc attcagttca tcgaccattt cttgtgcatg tttctttact tctcgcagtg
    24301 cttcttcccc atataaatgc tgtgatccta caacaaacca aaattctttt tctccaatat
    24361 tcaacataat tagccactcc ttttatttgt tttgtccgta atacgcattt tttccgtgtt
    24421 ttcgtaaata atgtttatct aaaatccgtt gaggcagctc ttctgaaaat gaattgagct
    24481 gtctggtaaa taaattcatc ttgcatactt catctagtac gactgcgttc attaccgcac
    24541 tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc cctggaattg
    24601 ctaagggatc aattttgcgt tctttgaatg tttctatgat aacttttcct gtttcatatt
    24661 cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc gatccataaa
    24721 aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt gcccagatcg
    24781 tcgcccatgt cgagtgagta tggacgattc ctccgatttg aggaaatgaa cgatataaaa
    24841 cagcatgagt aggcgtatcc gaagagggat tgagtttacc ttcaatgata tgattgtcca
    24901 agtccacaac gaccatgtcg tctgctgtca tctcttcata actgactccg ctaggtttga
    24961 taacgaaata tcgtgtttca ggatcaaaag cgctcacatt tccccatgta tatttgacga
    25021 gtccgtgttt aggcaagtca agatttgctt ggtatacttc ttctttcagt tgttccagca
    25081 ttttctatca ctcctttttc ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc
    25141 tcacggtaac gtctcataaa tatctgatag cctgctattt cttctgctga aggtgtgatg
    25201 gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga agttttctaa agacttccca
    25261 tgtcgatcca tcatataggc tgctaataac gcgattcccc acgctccacc ttctccggct
    25321 gtgtccatca ctgtaactgg tgcctccata gcagatgcta gaatccgctg tccaacttct
    25381 ggtgttttaa aaatcccccc atgccccact aaacgatcaa tttggacatt ctcagatttc
    25441 aatatatcca ttccgattct cattgcacca aaagcactgg ctaaatggat ccgcatgaaa
    25501 ttcgctaaat caaaacggct acctggtgta cgtacaaata atggccttcc ttcatttact
    25561 cctgtaatgt tttcaccgga aaaataacca taagaaagta atcccccgca atccgtatcg
    25621 ccttctagcg ctttgttgaa taataccgtg aataatttat ctctgcttat ctccataccc
    25681 aaagaattgg taaattcttc aaaaatactg acccaagcat tgatatccga agagcagtta
    25741 ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag tgaccatgtc gatttccgga
    25801 tggacatttt ctagttcttt ttctaatacg atcatagcga aggcggaagt accagccgaa
    25861 atgttccctg tccctttggc tacactgttc gtagcgacca taccggttcc tgcatcccct
    25921 tctggcggac aaaccgggat tcctggtctc aaatttcccg tagggtcgat cagataagcc
    25981 cctgattcgc ttaacttgcc agcttcttct cctgctacta gaactttagg caaaatgtga
    26041 tccaagtcaa tcgctacgtg cgctttttcg attaactgac caaaaatatt tttcatttct
    26101 tgatggtagg ttttcgttga aggatcgata ggaaacatgc ctgatgcatc tcctacgcct
    26161 agtactttct caccagtaag ctgccaatgg acataacctg caagcgttgt caaaaaatta
    26221 atttctttca catgttcttc tttattcaaa atagattgat acaaatgagc gatactccat
    26281 ctctgcggaa tattgtattg aaatttttcc gttaatattc gttctgcttc ttcagtgatt
    26341 gtatttcgcc aagtacgaaa tggtactagt aactgttcct tgctatcaaa tgctagataa
    26401 ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg taagattctc gccatagatt
    26461 tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca aatatcttct
    26521 aatgaatagg tccagatacc attttctagt ttgttttccc actcataact accagcggca
    26581 ataggagtaa aagatgaatc aatcaataca gctttgatcc tagttgatcc aaactcaatc
    26641 cctaaagatg tacgaccatc tatgacatct aacatacttt gcgcatttac ttccttcata
    26701 atgatttaac tccctccttc tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac
    26761 aatgattatt gtatactttt gtacgtacat gtcaagcagt tatttacaag aatacaataa
    26821 aaaacacttg ttttgtacgt acataagtta taaaatgaaa ttgcttacaa aatctgatca
    26881 accgccttct cgaaattcta gaaggagaga atacttatga aaaaagaaaa aaaagtttcc
    26941 tcatcgttta tttatttttt tggcgcattt ggcggcattc tatttggata tgatattggt
    27001 gttatgaccg gcgccttacc ttttttacag catgattggg gtttggcagg taaagcttct
    27061 ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg ctctttatct
    27121 ggtcttcttt ctgataagct tggacgacga aaaatgattc ttctttctgc acttattttt
    27181 atggctggtt ctgtcctttc tgcatctgcc cctcacaatg gttcttattt tctgattgca
    27241 gcaaggattc ttctaggttt agctgttggc gctgcatctg ccctagttcc tgcttatatg
    27301 tctgaaatgg ctcctgctag actaagggga agattgtcag ggatcaatca agtgatgatt
    27361 gcttctggca tgctcctttc ctatgttgca gattatttgc taaaagggtt gcctgaaacg
    27421 atggcttggc gcgtgatgtt agggctcgct gctgtccccg cgctgatttt atttttcggt
    27481 gttttagctc taccagagtc accgcgtttt cttatgcaat ctggccgctt ggaagaggca
    27541 aaaagagtat tgaactatat tcggacacca aaagaagctg aacaggaatt cgaacaaatc
    27601 cagctgaatg tcaaacaaga aaagacaacc ggaacttctt ggcatacttt gtttttagaa
    27661 aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag tattccagca atttcaagga
    27721 gctaatgcta ttttttacta tattccgctg atcgtagaaa aagcgactgg gcatgcagcg
    27781 agcgatgcat tgatgtggcc gatcatccaa ggaatcattt tagttgctgg ttctttgctc
    27841 tttttggtta ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg
    27901 atgggacttt ctttcatcct accagctgtt ttaggaactg tactggatgc tcatacaaat
    27961 tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat tttattcttg cacatgggct
    28021 ccactgacat gggtaatcgt aggcgaaata ttcccactag ctgtccgcgg gcgagcatct
    28081 ggcctagcct cttcttttaa ttggattggt tctttcctag ttggcttgct ttttcctgtg
    28141 atgactgctt ctatgtctca agaaatcgtt ttcggcattt ttgggatcat ctgcttttta
    28201 ggggttttat ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc
    28261 gaacaaagtg catctaaaaa aacttatcct aaacgtattt ctatccatca ttcttaaaga
    28321 ggttgtgaca gaagtggtca gcttcaagaa ataagaagga accgcttagt ttccagtcac
    28381 aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa tattgtgact tgtgaccagt
    28441 tggtaggaga acaccgttta gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg
    28501 acaaaagctc tcacaaacat tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa
    28561 cccgtctctt atttttcgtt ttctttcact gtttcccgtt caactagtac aggttcatag
    28621 agaatatttg gctgtgcctg tcctttttca atcgttgtga cgatccaccg tgcggcatct
    28681 tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt ttttattccc taaatggctt
    28741 aaagaagaat cgtctgttcc tacgatcgat aggtcatctg gaatatgata gccattttct
    28801 gtcaacttat ctagcattaa actagctatt tgatcgttat agcaaatgat gccatctaaa
    28861 gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg
    28921 gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat actgttcaca cgcgctgata
    28981 aaacctttca tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc
    29041 tgatgtcctt tttttatcaa atattctgct gccataaaac cgactttcac atcatctaaa
    29101 caaatataag aggcatttaa ctcttcataa gcagcattga tcatcaccat cggaatgcct
    29161 tgttctctca aagcaacata aagtgctaag tttgggttat actgattact tttcgtaggc
    29221 tcaacgatca aaccatctac cccataagca atcattttct tcaaacactc tttctccaat
    29281 tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc cagattctcg taattcattt
    29341 tcgattcctc ggataatcga cggaaaaata taatcagaga gatatgtggt gatgacaccg
    29401 atcgtttttg ttctgttcaa tggtgttttt tctttcttat agctgtcatc tacatatgtg
    29461 cccgaccctt tttctttacg cagatagccc tcatttacca acacagcgat tgcttgccga
    29521 atcgtatgtc tgctgacgtc atatatttct tgcagcttga attctgaggg aatggttgta
    29581 tttacttcgt attcctcacg aaggatcttt tgttttatct cttctgcaat atcttgatat
    29641 tttgccattt gccttgcccc tttttatttg tacgtacata tatcataacg agtctgcaag
    29701 gtttattcaa gcaaaaatca aggtttcttg tgctcataag gcatacggaa caggttcacc
    29761 aaattctgga aatccttgct cattccacgt aaagcattgc gcgttcgcgt gccgattagg
    29821 attgtctaac ggatcccctt tttggttctt ctctggtcga gcatgataga tcaaagtatc
    29881 aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt gcttattttt
    29941 ttcagaagaa acgaatactg gctccgataa tttatgccat gattctttta ataaaagatt
    30001 cttgtgctca tcagcccaca gcaggcccat ggcatagttt tcatctgttg cactaccaga
    30061 atacgtaatg aatattttcc cattgcgagt aaggactgct ggaccttcat ttactgaaaa
    30121 accccgtttt tcccattcat actcaggtat agaaaggagg atctgcttcc cctttaacgt
    30181 ccaaggatta tccatttcgg atagataaag attagaattc ccagtaattt caggatcttg
    30241 ttgggcccac acataatata gctttccttg agaagagaaa gttgttccgt ctaaactgaa
    30301 agtttccctt ttcgttttta tttgtccctt ttctatccaa tctgtattca ttggatctat
    30361 atgctgattt tcaagtacga acatgcggtg atgatgagat tcatcacgta tagttttatg
    30421 attgcttgca gcaaagtaaa tataccattt tccattgata taatggactt caggtgccca
    30481 gatcaattca ctcatcggtc cttcttcatg agcctgccat acaatcaggc ttgcagcatg
    30541 ttctaattca ttcagtgact ttgcccttct taattcaatc acttggtagc ctggaaccga
    30601 tcctgtaaaa taataataac catctgtatg tttatatacc caaggatctg cccgttgccg
    30661 aacgatagga ttattataaa gtgtcttcat cataacaaat ttcctcacat tttctttatt
    30721 ctaattacat tccaagagta tttttctatc cctacattta attcgtcatg tttttctatt
    30781 gaaaaatagt ttatttcagg ttttattttt tctggttctt cacgtgtatt tttgtccaac
    30841 agatcgctac tggttaatgt gtagtgctct ccttgatcta gcaaattgaa atcatgttca
    30901 atcgttatct tttgttgttc atttgtgcga ttgatcaaga aaatagtcag ctgattgccg
    30961 ttatctacga tcacactgtc cacataagga acatttttta tcttttcaca atcataaaca
    31021 gggcaaatga tttgttgttt caatacttga cctttagcat atttcgataa gtgttgcatc
    31081 acataataag ttggattcct ccatgcttgt cctttagcat ctgtcagtat taaaggtatt
    31141 gtgtttacta acaatgattg acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc
    31201 agccctaatg aaccaaataa taatgtatct tccatagtaa agtaacacca atcgatggga
    31261 ctaccgactt caaaatccct atgtcttttt tccggttggg catgtacatt ccattcatca
    31321 aacgctagat acattgtttt ctcacttctc aaatatcctt ttacataatc acaagtagca
    31381 acgatttctt caatttgacg atcgaatcga taagcttttg caaggtatgt cgctgtatca
    31441 tcacgctcat ttttaggagg tttagtcaag tcttcttcat catacttatc aatatagtta
    31501 tgcaaagcta aataatctac attctcataa gcttcttcta atgcgatacg atcccattca
    31561 gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc
    31621 actaacttca tgacttttga ggcttcattt gctaacctac catattcata agcactctta
    31681 gcccccactt gccatggacc gtctagctca ttccctaaac accataattt aacgccaaaa
    31741 ggcttttttt gcccattttt tattcttaaa tcactccaga aggttccttt tggaaaatta
    31801 caatattcta ctaaattttt tgcttcatca attccttttg ttcctaaatt caccgtgaat
    31861 atcggtttcg cttgtacatg actgatccat ttaaaaaatt cgtggagtcc aaaagaattt
    31921 ggttctatac ctcgccatgc taaatcaatt ttttttggtc gttgttcttt cctacctaca
    31981 gtatccaccc aatcataacc agatgtaaag tttcctccag gatagcgaat gacacctaat
    32041 tctaattcct ttacataaga gaggacatct tgtctaaacc cgtcaccatc agctgtttca
    32101 tggcccggtt cataaatccc tgtatagacg acacttccca tatgctcaac aaatgagcca
    32161 aacagccgag aactgatctc tcctaccgta aaatcctctc tgatttttac tttcacttct
    32221 tcgtccaaca gctttcccta cctttcattt cctatagtca agataataga tacaagtttc
    32281 catacgccac cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat
    32341 cctaataaca atacgcaaca aagaatattt ctcaaaaaat caaaaaatac tttccatgca
    32401 ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt cagaaatcac atatatccag
    32461 gataaaagaa acatggaata aataagtaaa actacatatc cactaat
//
LOCUS       DAXWLB010000010        32187 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXWLB010000010 DAXWLB010000000
VERSION     DAXWLB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.010"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..111)
                     /locus_tag="ACGE4H_000387"
     CDS             complement(<1..111)
                     /locus_tag="ACGE4H_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFF8363830.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQ"
     gene            complement(207..1511)
                     /locus_tag="ACGE4H_000388"
     CDS             complement(207..1511)
                     /locus_tag="ACGE4H_000388"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8363831.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(1508..2017)
                     /locus_tag="ACGE4H_000389"
     CDS             complement(1508..2017)
                     /locus_tag="ACGE4H_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363832.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(2192..4327)
                     /locus_tag="ACGE4H_000390"
     CDS             complement(2192..4327)
                     /locus_tag="ACGE4H_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFF8363833.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(4410..6065)
                     /locus_tag="ACGE4H_000391"
     CDS             complement(4410..6065)
                     /locus_tag="ACGE4H_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8363834.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(6067..6381)
                     /locus_tag="ACGE4H_000392"
     CDS             complement(6067..6381)
                     /locus_tag="ACGE4H_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8363835.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            6549..6809
                     /locus_tag="ACGE4H_000393"
     CDS             6549..6809
                     /locus_tag="ACGE4H_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFF8363836.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            6823..7137
                     /locus_tag="ACGE4H_000394"
     CDS             6823..7137
                     /locus_tag="ACGE4H_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFF8363837.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(7867..8421)
                     /locus_tag="ACGE4H_000395"
     CDS             complement(7867..8421)
                     /locus_tag="ACGE4H_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFF8363838.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(8547..10476)
                     /locus_tag="ACGE4H_000396"
                     /pseudo
     CDS             complement(8547..10476)
                     /locus_tag="ACGE4H_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(10782..11498)
                     /locus_tag="ACGE4H_000397"
     CDS             complement(10782..11498)
                     /locus_tag="ACGE4H_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8363839.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(11499..13322)
                     /gene="glpO"
                     /locus_tag="ACGE4H_000398"
     CDS             complement(11499..13322)
                     /gene="glpO"
                     /locus_tag="ACGE4H_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFF8363840.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(13336..14832)
                     /gene="glpK"
                     /locus_tag="ACGE4H_000399"
     CDS             complement(13336..14832)
                     /gene="glpK"
                     /locus_tag="ACGE4H_000399"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFF8363841.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(14968..16428)
                     /locus_tag="ACGE4H_000400"
     CDS             complement(14968..16428)
                     /locus_tag="ACGE4H_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8363842.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(16418..17713)
                     /locus_tag="ACGE4H_000401"
     CDS             complement(16418..17713)
                     /locus_tag="ACGE4H_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8363843.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(18045..18722)
                     /locus_tag="ACGE4H_000402"
     CDS             complement(18045..18722)
                     /locus_tag="ACGE4H_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFF8363844.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(18752..19843)
                     /locus_tag="ACGE4H_000403"
     CDS             complement(18752..19843)
                     /locus_tag="ACGE4H_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFF8363845.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(19944..20378)
                     /locus_tag="ACGE4H_000404"
     CDS             complement(19944..20378)
                     /locus_tag="ACGE4H_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFF8363846.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(20962..22419)
                     /locus_tag="ACGE4H_000405"
     CDS             complement(20962..22419)
                     /locus_tag="ACGE4H_000405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8363847.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(22416..24311)
                     /locus_tag="ACGE4H_000406"
     CDS             complement(22416..24311)
                     /locus_tag="ACGE4H_000406"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8363848.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(24369..26144)
                     /locus_tag="ACGE4H_000407"
     CDS             complement(24369..26144)
                     /locus_tag="ACGE4H_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8363849.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(26194..27690)
                     /locus_tag="ACGE4H_000408"
     CDS             complement(26194..27690)
                     /locus_tag="ACGE4H_000408"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8363850.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(27884..29506)
                     /locus_tag="ACGE4H_000409"
     CDS             complement(27884..29506)
                     /locus_tag="ACGE4H_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8363851.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(29834..31147)
                     /locus_tag="ACGE4H_000410"
     CDS             complement(29834..31147)
                     /locus_tag="ACGE4H_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8363852.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(31392..31616)
                     /locus_tag="ACGE4H_000411"
     CDS             complement(31392..31616)
                     /locus_tag="ACGE4H_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363853.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(31636..32058)
                     /locus_tag="ACGE4H_000412"
     CDS             complement(31636..32058)
                     /locus_tag="ACGE4H_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8363854.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         9270 a         6917 c         5327 g        10673 t
ORIGIN      
        1 ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac
       61 agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca tccttactac
      121 tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc
      181 tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc
      241 ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc
      301 gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt
      361 cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca
      421 aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga
      481 tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac
      541 cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga
      601 tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt
      661 tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca
      721 ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct
      781 gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta
      841 tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg
      901 cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg
      961 acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc
     1021 acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat
     1081 ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg
     1141 atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt
     1201 tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc
     1261 atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc
     1321 gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat
     1381 aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat cggataagaa
     1441 acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg
     1501 attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat
     1561 ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc
     1621 caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt
     1681 catattcaaa agatttattt acttttttgt atagatttcg attgttccac ctagtcaata
     1741 caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca
     1801 gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg
     1861 ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca
     1921 ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca
     1981 aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt
     2041 tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt
     2101 tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta
     2161 tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc
     2221 ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat
     2281 cgttttcaaa tggatataag attacatgat caaattccag tccttttgct agactcaaag
     2341 gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt
     2401 gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa
     2461 tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt
     2521 ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga
     2581 aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg
     2641 gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga
     2701 ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata
     2761 gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg
     2821 gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa
     2881 cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa
     2941 acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt
     3001 ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca
     3061 atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag
     3121 caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg
     3181 ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga
     3241 tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat
     3301 gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac
     3361 gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt
     3421 ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc
     3481 tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata
     3541 gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac
     3601 gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac
     3661 ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca
     3721 tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag
     3781 acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa
     3841 taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt
     3901 aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg
     3961 tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa
     4021 cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg
     4081 cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa
     4141 aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt
     4201 tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt
     4261 tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt
     4321 tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta
     4381 agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt
     4441 cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc
     4501 ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac gatatattct
     4561 tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct
     4621 tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct
     4681 tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg
     4741 ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca
     4801 tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct
     4861 aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg
     4921 ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt
     4981 gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt
     5041 ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca
     5101 ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga
     5161 cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca
     5221 acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact
     5281 aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga
     5341 cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc
     5401 aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct
     5461 tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt tggaagaata
     5521 tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt
     5581 tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca
     5641 tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt
     5701 atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac
     5761 tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg
     5821 gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc
     5881 gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg
     5941 caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt
     6001 aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt
     6061 ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt
     6121 cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga
     6181 ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa
     6241 tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc
     6301 aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat
     6361 tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg
     6421 ggtattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt tttttcacta
     6481 ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag
     6541 aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac
     6601 aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc
     6661 aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc
     6721 tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac
     6781 aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat
     6841 attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct
     6901 tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac
     6961 tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct
     7021 atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca
     7081 ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca
     7141 gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc
     7201 tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag
     7261 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat taccgcagat
     7321 gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg acctaagtat
     7381 tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc taataccgaa
     7441 ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt agtttcgagt
     7501 attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg gcttaatacc
     7561 gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg
     7621 agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt tgagctaata
     7681 ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc atttaactcc
     7741 gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt tggcttatta
     7801 tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat aggtcacacc
     7861 ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga caaaagattg
     7921 taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg gtaattctgg
     7981 gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt tgccatacca
     8041 cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta caaacataaa
     8101 gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat accatgccat
     8161 agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca ccttccctaa
     8221 gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag acaaacctga
     8281 gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat aaagataaat
     8341 cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta tttctttacg
     8401 ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt tctttttatt
     8461 ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct ttacagacac
     8521 tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac tcttctttct
     8581 tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga tcctcgctct
     8641 aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa atcgtaacct
     8701 gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg cacgtggatt
     8761 atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata aactaacgct
     8821 tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg acgttctttt
     8881 ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt ctgtgtccga
     8941 taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga catatccatt
     9001 ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca actgatattt
     9061 aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc
     9121 ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata
     9181 catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc
     9241 aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt
     9301 ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc
     9361 gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc
     9421 gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc
     9481 atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac
     9541 cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt
     9601 gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc
     9661 taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc
     9721 agtcaatgcc gctaggactt gatattcttc actgatttct tcagctttta acaactgata
     9781 gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga
     9841 ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata
     9901 gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg
     9961 caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac
    10021 tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat
    10081 caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt
    10141 ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc
    10201 atattcgatc aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc
    10261 atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc
    10321 ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc
    10381 gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg
    10441 atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat
    10501 acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa
    10561 aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc
    10621 ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta gataccagct
    10681 acactcacaa aacatacccc attcttctag gtcttattta ttttccactt aactatagca
    10741 agacacattt tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag
    10801 acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc
    10861 caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag
    10921 tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag
    10981 atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct
    11041 agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa
    11101 ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca
    11161 ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact
    11221 tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct
    11281 agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt
    11341 gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt
    11401 ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca
    11461 ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc
    11521 cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct
    11581 tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc
    11641 tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa
    11701 accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga
    11761 taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca
    11821 gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct
    11881 gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta
    11941 gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc
    12001 aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa
    12061 ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca
    12121 cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc
    12181 ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta
    12241 tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag
    12301 cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc
    12361 aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc
    12421 gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca
    12481 tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca
    12541 actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag
    12601 tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg
    12661 atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat
    12721 aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc agcctttttg
    12781 atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct
    12841 tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta
    12901 taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact
    12961 ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt
    13021 ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg
    13081 tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg
    13141 gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt
    13201 cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac
    13261 agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac
    13321 atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt
    13381 gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata
    13441 aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc
    13501 tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc
    13561 tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg
    13621 aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat
    13681 cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat
    13741 agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac
    13801 ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg
    13861 caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc
    13921 ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt
    13981 tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt
    14041 attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc
    14101 tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata
    14161 aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa
    14221 aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga
    14281 gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt
    14341 tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat
    14401 ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa
    14461 aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg
    14521 ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc
    14581 acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa
    14641 cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca
    14701 actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt
    14761 cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt
    14821 tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc
    14881 atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca
    14941 gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg tctctattct
    15001 tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg
    15061 ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact
    15121 tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat
    15181 acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa
    15241 atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc
    15301 agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt
    15361 taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc
    15421 gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc
    15481 cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat
    15541 gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa
    15601 tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta
    15661 tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg
    15721 ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt
    15781 ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga
    15841 tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg
    15901 tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata
    15961 aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa
    16021 ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt
    16081 aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat
    16141 cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc
    16201 gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat
    16261 tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac
    16321 atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat
    16381 caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg
    16441 ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt
    16501 cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt
    16561 ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata
    16621 gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg
    16681 tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga
    16741 aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc
    16801 cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat
    16861 acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt
    16921 ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta
    16981 attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat
    17041 agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa
    17101 cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg
    17161 tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt
    17221 gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa
    17281 ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt
    17341 taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag
    17401 tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt
    17461 ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt
    17521 cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat
    17581 tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg
    17641 ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa
    17701 ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc
    17761 agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat
    17821 caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta
    17881 attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa
    17941 actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt
    18001 tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct
    18061 tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc
    18121 ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct
    18181 ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct
    18241 tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta
    18301 actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag
    18361 atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg
    18421 ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta
    18481 tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc
    18541 atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct
    18601 gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa
    18661 cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc
    18721 attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat
    18781 ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt
    18841 catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc
    18901 caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc
    18961 caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg
    19021 atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca
    19081 gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga
    19141 tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag
    19201 catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat
    19261 tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt
    19321 ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc
    19381 tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc
    19441 gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc
    19501 cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg
    19561 cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag
    19621 cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa
    19681 taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca
    19741 ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt
    19801 gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa
    19861 ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat
    19921 gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt
    19981 gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt
    20041 tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact
    20101 aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca
    20161 aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt
    20221 ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt
    20281 ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa
    20341 agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac
    20401 atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg
    20461 tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa
    20521 ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa
    20581 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa
    20641 actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa
    20701 taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat
    20761 ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc
    20821 acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg
    20881 aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc
    20941 acagtccctt atttcctttt attataggat ttcgccattt gattggatga cttgtctgta
    21001 ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc
    21061 aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat
    21121 gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg
    21181 ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc
    21241 tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc
    21301 atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact
    21361 tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga ccccttccca
    21421 attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac
    21481 agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc
    21541 ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa
    21601 caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg
    21661 aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca
    21721 agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg
    21781 ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac
    21841 acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac
    21901 tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc
    21961 tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct caatcccata
    22021 ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc
    22081 ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc
    22141 catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct
    22201 tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat
    22261 catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc
    22321 agacacagat aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt
    22381 cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct
    22441 agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta
    22501 tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct
    22561 acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag
    22621 tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct
    22681 tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca
    22741 gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta
    22801 gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt
    22861 aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt
    22921 agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca
    22981 ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct
    23041 gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt
    23101 ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata
    23161 aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt
    23221 actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg
    23281 tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact
    23341 aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca
    23401 gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat
    23461 aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt
    23521 tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt
    23581 gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag
    23641 gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc
    23701 atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc
    23761 aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta
    23821 gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc
    23881 aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg
    23941 ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg
    24001 gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca
    24061 gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta
    24121 ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct
    24181 tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc
    24241 cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac
    24301 ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta
    24361 tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc
    24421 ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt
    24481 atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac
    24541 aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat tagttgctga
    24601 atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc
    24661 agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc
    24721 tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa
    24781 ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt
    24841 ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat actttgttga
    24901 atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc
    24961 ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca
    25021 gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct
    25081 aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc
    25141 atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac
    25201 gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct
    25261 atttctttgt ttttttcatc attaatcatc aaataattta actctaatag tcgattttca
    25321 attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg
    25381 agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct
    25441 cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca
    25501 atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg
    25561 gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca
    25621 acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc
    25681 gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt
    25741 tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca
    25801 tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa
    25861 tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat
    25921 tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca
    25981 agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat
    26041 tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt
    26101 ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc
    26161 aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag
    26221 gtttttacca ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct
    26281 cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct
    26341 catttcccct gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat
    26401 ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca
    26461 acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt
    26521 ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa
    26581 ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt
    26641 atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc
    26701 gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc
    26761 tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg
    26821 ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga
    26881 accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca
    26941 cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt
    27001 cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg
    27061 ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa
    27121 agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa
    27181 gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata
    27241 ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc
    27301 atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt
    27361 ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa
    27421 acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc
    27481 atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt
    27541 ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa
    27601 tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa
    27661 aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct
    27721 tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga
    27781 agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac
    27841 gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca
    27901 caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat
    27961 ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca
    28021 tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct
    28081 caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca
    28141 aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg
    28201 cattgatttc tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag
    28261 catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga
    28321 ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga
    28381 ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact
    28441 cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct
    28501 tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat
    28561 cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg
    28621 ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt
    28681 cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag
    28741 gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca
    28801 gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc
    28861 cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa
    28921 agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct
    28981 gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc
    29041 ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct
    29101 cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt
    29161 tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga
    29221 tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac
    29281 cgaattctgc catgattgct tgataattac tgatatcata cccattgtca tcattcggag
    29341 actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc
    29401 tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg
    29461 gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat
    29521 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
    29581 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
    29641 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat
    29701 ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac
    29761 ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga
    29821 tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca
    29881 gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
    29941 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
    30001 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
    30061 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
    30121 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
    30181 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
    30241 aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
    30301 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
    30361 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
    30421 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
    30481 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
    30541 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
    30601 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
    30661 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac
    30721 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
    30781 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
    30841 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
    30901 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa acacagccga
    30961 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
    31021 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
    31081 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
    31141 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
    31201 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
    31261 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
    31321 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
    31381 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
    31441 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
    31501 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
    31561 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
    31621 tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
    31681 agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc
    31741 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
    31801 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
    31861 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
    31921 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
    31981 catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg
    32041 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
    32101 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
    32161 aaaacggcaa tgaatgccgc tcttctt
//
LOCUS       DAXWLB010000011        31038 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXWLB010000011 DAXWLB010000000
VERSION     DAXWLB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.011"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(73..792)
                     /locus_tag="ACGE4H_000413"
     CDS             complement(73..792)
                     /locus_tag="ACGE4H_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8363855.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(923..2074)
                     /locus_tag="ACGE4H_000414"
     CDS             complement(923..2074)
                     /locus_tag="ACGE4H_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFF8363856.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2291..3031)
                     /locus_tag="ACGE4H_000415"
     CDS             complement(2291..3031)
                     /locus_tag="ACGE4H_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8363857.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE4H_000416"
     CDS             complement(3035..3391)
                     /gene="rsfS"
                     /locus_tag="ACGE4H_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFF8363858.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE4H_000417"
     CDS             complement(3415..4008)
                     /gene="yqeK"
                     /locus_tag="ACGE4H_000417"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFF8363859.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3992..4642)
                     /locus_tag="ACGE4H_000418"
     CDS             complement(3992..4642)
                     /locus_tag="ACGE4H_000418"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFF8363860.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE4H_000419"
     CDS             complement(4652..4963)
                     /gene="yhbY"
                     /locus_tag="ACGE4H_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFF8363861.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE4H_000420"
     CDS             complement(4979..6088)
                     /gene="yqeH"
                     /locus_tag="ACGE4H_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFF8363862.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6085..6612)
                     /locus_tag="ACGE4H_000421"
     CDS             complement(6085..6612)
                     /locus_tag="ACGE4H_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFF8363863.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6839..7624)
                     /locus_tag="ACGE4H_000422"
     CDS             complement(6839..7624)
                     /locus_tag="ACGE4H_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFF8363864.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE4H_000423"
     CDS             complement(7617..8486)
                     /gene="accD"
                     /locus_tag="ACGE4H_000423"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFF8363865.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8487..9866)
                     /locus_tag="ACGE4H_000424"
     CDS             complement(8487..9866)
                     /locus_tag="ACGE4H_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFF8363866.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE4H_000425"
     CDS             complement(9874..10293)
                     /gene="fabZ"
                     /locus_tag="ACGE4H_000425"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8363867.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE4H_000426"
     CDS             complement(10318..10794)
                     /gene="accB"
                     /locus_tag="ACGE4H_000426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFF8363868.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE4H_000427"
     CDS             complement(10797..12032)
                     /gene="fabF"
                     /locus_tag="ACGE4H_000427"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFF8363869.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE4H_000428"
     CDS             complement(12051..12788)
                     /gene="fabG"
                     /locus_tag="ACGE4H_000428"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFF8363870.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE4H_000429"
     CDS             complement(12800..13717)
                     /gene="fabD"
                     /locus_tag="ACGE4H_000429"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8363871.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13824..14048)
                     /locus_tag="ACGE4H_000430"
     CDS             complement(13824..14048)
                     /locus_tag="ACGE4H_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8363872.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14101..15063)
                     /locus_tag="ACGE4H_000431"
     CDS             complement(14101..15063)
                     /locus_tag="ACGE4H_000431"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFF8363873.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15073..15513)
                     /locus_tag="ACGE4H_000432"
     CDS             complement(15073..15513)
                     /locus_tag="ACGE4H_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8363874.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15819..16784
                     /locus_tag="ACGE4H_000433"
     CDS             15819..16784
                     /locus_tag="ACGE4H_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8363875.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(16841..17071)
                     /locus_tag="ACGE4H_000434"
     CDS             complement(16841..17071)
                     /locus_tag="ACGE4H_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFF8363876.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17157..18044)
                     /locus_tag="ACGE4H_000435"
     CDS             complement(17157..18044)
                     /locus_tag="ACGE4H_000435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8363877.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18133..19356)
                     /locus_tag="ACGE4H_000436"
     CDS             complement(18133..19356)
                     /locus_tag="ACGE4H_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8363878.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19373..20419)
                     /locus_tag="ACGE4H_000437"
     CDS             complement(19373..20419)
                     /locus_tag="ACGE4H_000437"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFF8363879.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(20583..22310)
                     /gene="ptsP"
                     /locus_tag="ACGE4H_000438"
     CDS             complement(20583..22310)
                     /gene="ptsP"
                     /locus_tag="ACGE4H_000438"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFF8363880.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(22310..22576)
                     /locus_tag="ACGE4H_000439"
     CDS             complement(22310..22576)
                     /locus_tag="ACGE4H_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFF8363881.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23055..25289
                     /locus_tag="ACGE4H_000440"
     CDS             23055..25289
                     /locus_tag="ACGE4H_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8363882.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25437..25757
                     /locus_tag="ACGE4H_000441"
     CDS             25437..25757
                     /locus_tag="ACGE4H_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8363883.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(25962..26186)
                     /locus_tag="ACGE4H_000442"
     CDS             complement(25962..26186)
                     /locus_tag="ACGE4H_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFF8363884.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(26384..27964)
                     /locus_tag="ACGE4H_000443"
     CDS             complement(26384..27964)
                     /locus_tag="ACGE4H_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFF8363885.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(28365..29741)
                     /locus_tag="ACGE4H_000444"
     CDS             complement(28365..29741)
                     /locus_tag="ACGE4H_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8363886.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(29869..30957)
                     /locus_tag="ACGE4H_000445"
     CDS             complement(29869..30957)
                     /locus_tag="ACGE4H_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8363887.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT         8724 a         6696 c         5314 g        10304 t
ORIGIN      
        1 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
       61 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
      121 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
      181 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
      241 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
      301 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
      361 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
      421 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
      481 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
      541 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
      601 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
      661 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
      721 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
      781 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
      841 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
      901 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
      961 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     1021 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     1081 ttccaacatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     1141 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     1201 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
     1261 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
     1321 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaat
     1381 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
     1441 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
     1501 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
     1561 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
     1621 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
     1681 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
     1741 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
     1801 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
     1861 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
     1921 tatcgatgat agccggttcg cctcgttgca aaaaattccc gctcattaca gcaatgacga
     1981 cctctgctcc tgttgttttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
     2041 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
     2101 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
     2161 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
     2221 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
     2281 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
     2341 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
     2401 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
     2461 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
     2521 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
     2581 attctggaaa aatttcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
     2641 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
     2701 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
     2761 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
     2821 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
     2881 aggcaactgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
     2941 gtaaatgacg tttcgaaaaa gctagccatt gctgataaag tgtttcgtcc atcacttcat
     3001 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
     3061 ctagcggagc atctgaccat agtttttcca gattatagaa acttcgttct ggtgcatgga
     3121 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
     3181 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
     3241 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
     3301 catttcgaat gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaaccg
     3361 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
     3421 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
     3481 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
     3541 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
     3601 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
     3661 tgcatgaaga atttctacat cggtgattcc taattcccgc tctacaaacg aagcgccaac
     3721 aagaccatgc cagattgcat tattataatt caataactct ggatcaaaac cgtctcttac
     3781 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
     3841 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
     3901 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
     3961 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
     4021 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
     4081 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
     4141 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
     4201 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
     4261 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
     4321 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccacag
     4381 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
     4441 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
     4501 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
     4561 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
     4621 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
     4681 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
     4741 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
     4801 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
     4861 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
     4921 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
     4981 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
     5041 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
     5101 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
     5161 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
     5221 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
     5281 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
     5341 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
     5401 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
     5461 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
     5521 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
     5581 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
     5641 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
     5701 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
     5761 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
     5821 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttctgcccta
     5881 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg atctcattat
     5941 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
     6001 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
     6061 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
     6121 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
     6181 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
     6241 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
     6301 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
     6361 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
     6421 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
     6481 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
     6541 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
     6601 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
     6661 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
     6721 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
     6781 acaaaaaaca actaagcgag caagaccatt ctttactctt tgcttagttg ttaaacacct
     6841 aatattggcg gaacctcttg tatctttttt ccagtaattc atgctggtcg aatcgaacaa
     6901 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
     6961 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
     7021 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
     7081 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
     7141 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
     7201 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
     7261 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aatgtaatca
     7321 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
     7381 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
     7441 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
     7501 ctgcaaacaa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
     7561 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
     7621 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgcttc
     7681 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
     7741 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
     7801 ccgatcaagc tttggggttc tgctagaatg atatcgcctt ccattgcaaa actggcagtt
     7861 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
     7921 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
     7981 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
     8041 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
     8101 tccatcactc caataatgaa gggaatccct tgaatggttg ctttaccagt gaggactgct
     8161 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
     8221 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
     8281 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
     8341 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
     8401 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
     8461 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
     8521 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcaggg
     8581 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
     8641 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
     8701 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
     8761 acccgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
     8821 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
     8881 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
     8941 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
     9001 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
     9061 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
     9121 tgtgagatca ctacagaagg agattcttcc agtacttttt gattattacg ttgcaaagaa
     9181 caatctcgtt ctcctaaatg aatgacattg ccaaaatgat cacctaaaat ctgaacttct
     9241 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
     9301 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
     9361 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
     9421 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
     9481 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
     9541 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
     9601 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
     9661 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
     9721 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
     9781 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
     9841 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
     9901 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
     9961 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    10021 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    10081 taatgcaact gctcctgctt gagccatcgc ttcaacaatt aatacccctg gcataacagg
    10141 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    10201 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    10261 tggaatgatt tcttttattt cttgtatgtt cattgatttc cctctttcct gctttgctta
    10321 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    10381 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    10441 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    10501 gtcgggcgcc ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    10561 aaatactgcc gattcttttt ctttcattgt ttctggagca gttgttgctt gttcaggtat
    10621 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    10681 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    10741 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    10801 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    10861 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    10921 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    10981 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    11041 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    11101 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    11161 tgtacaccag cttcttccat cgcaagcttc attgctttag acgcaccaga accatctgga
    11221 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaatgat ttctccatag
    11281 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac acctgctcct
    11341 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    11401 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    11461 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    11521 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    11581 gcacctacgc gcaaagcaat atttccagct gccatgttag caatcgccat tggtacgaac
    11641 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    11701 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    11761 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    11821 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    11881 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    11941 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    12001 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    12061 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    12121 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    12181 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    12241 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    12301 aatcctacta cgccagcttt actagctgca taatttgcct gaccaatatt tccaatcaac
    12361 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    12421 acgtgctgcg tcatattgaa ggttccaatc aaattgatat caagacattt tttgaaatct
    12481 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    12541 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    12601 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    12661 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    12721 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    12781 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    12841 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    12901 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    12961 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    13021 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    13081 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    13141 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    13201 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    13261 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    13321 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    13381 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    13441 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    13501 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    13561 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    13621 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    13681 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    13741 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    13801 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    13861 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    13921 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    13981 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    14041 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    14101 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    14161 tttctgctgt tttcccaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    14221 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    14281 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    14341 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    14401 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    14461 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    14521 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    14581 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    14641 tgtttcacca ccaatcacta gtcccttagt tgagccgcag cggatcattt tttcagccat
    14701 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    14761 tgctcccaat ttcccttgta cttgtgctgc tacagatggc gtcgtgtaat cgggcgacat
    14821 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    14881 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    14941 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    15001 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    15061 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    15121 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    15181 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    15241 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    15301 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    15361 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    15421 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    15481 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    15541 tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag
    15601 atcatttttt tactatatga gcttttttta agattttact catttaaaac aactgataaa
    15661 tcagcattta taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa
    15721 agtattttct gtgaacagaa ctatagaatt ccttaaaact tgtccattat ttcaaaattt
    15781 gttatagtct ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa
    15841 ctctccccat gctgccgggt gtctatctta tgaaagaccg tgatggtaag gtcatctatg
    15901 ttggtaaagc aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc
    15961 attcaaaaaa agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag
    16021 acacggaatt agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt
    16081 ataaccgaca aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat
    16141 ttgtattaac agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa
    16201 tgccgtctat tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta
    16261 gtttgttagc tttgcgagcc cagcttcctg acctacagga aatgactttt gaacagaaaa
    16321 aaaaggaact ccaagggctg tttcaaggcc aaaataaaaa gctactaact tacttgaaaa
    16381 aaagacaaca acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag
    16441 atatcgaatt agttacttat tttattagac ggatccagga acaaaagcat tttttaagaa
    16501 caccatcgct caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc
    16561 tcgtttgtta tggtcaactt gcagaaacaa tgattgcctc tgctgacgtt tctcctgact
    16621 tctattatga aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa
    16681 tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtctgaaa
    16741 ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct
    16801 tttaggcatc ttcaacttca aatgtcaatt attctaaaat ttattggatt aagtcataga
    16861 tttccatcgc tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag
    16921 ttaattcaaa tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt
    16981 tttcaaaacg gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt
    17041 ttatgattgc gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta
    17101 tattgtatca aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat
    17161 gatttttttt ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg
    17221 tcattctcaa aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct
    17281 ggcaatacaa ggtccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa
    17341 tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt
    17401 aaagaccagt tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt
    17461 ccgttttttt gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg
    17521 atctgattag ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc
    17581 gtaccaaatt ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc
    17641 atgatcgtca gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac
    17701 gcatctgcgc ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt
    17761 gtagtcaagt caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca
    17821 tagtagattt cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt
    17881 tctgctaccg cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct
    17941 tttgcttttg tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg
    18001 acagcacttc ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa
    18061 aaatcaaaaa tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa
    18121 aatgtttgaa ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc
    18181 ttcttcttct tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc
    18241 gatcatagct tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt
    18301 tggatcttta ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc
    18361 ggtttcaaaa gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc
    18421 ttccacgaca caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc
    18481 tgaagtagaa gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt
    18541 gtccacttct cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc
    18601 taactcttct acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga
    18661 gatgacctga ggaaacccat taatcaaggc ttggatattt ttttcataag aaatcctgct
    18721 taaagaaaga atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat
    18781 atcctgctga gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac
    18841 gcgaagcggt gctattacac catagctacg taatgtatca ataacacgtt cacttggaca
    18901 aactacacct gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct
    18961 gatcactttt cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg
    19021 gatcagaggt attttcaact tcttagctac catcttccca agtatccctg ctccgaattc
    19081 tgtatgggta tgaataagat ctagttctag ttctttggca accaaataag cataccacat
    19141 gcctctaaca acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat
    19201 cacatctttt tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg
    19261 cccatgtctt tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg
    19321 cggaaagtag gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct
    19381 tgacctgatt tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata
    19441 catttttaag ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg
    19501 cttgtgtcgt actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt
    19561 cgtttgtctt ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa
    19621 tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg
    19681 tctcttcata agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct
    19741 ctccacgaat gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc
    19801 ggatgttccg aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg
    19861 gcatcttctt agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga
    19921 cacaaatgat tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt
    19981 tcattggatc agggcgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca
    20041 cacggatact atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt
    20101 ccgctttttg gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc
    20161 cgataaagga atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc
    20221 cttctcttct ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca
    20281 ggatgtcata atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac
    20341 gtttttgatg atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa
    20401 aatataataa taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca
    20461 aactgtcact caaacttaag ccctattttt atctattctt actgacaaaa acgttttctt
    20521 aaaaaagaac aaaaaaactg gcgtagctct tttacaagcc acaccagctt ttgatacgtt
    20581 gtttatttta catattcatg aacaagttca acgacttctt ccatcgtatc gcattctttc
    20641 aatgcacgat cagcaagttc agccattttc gctgtgtcca agcgtttcat taaactacgc
    20701 gttttaagaa tagatgtcgc actcattgag aactcatcta gacccatacc tactagcaat
    20761 ggaacagctg tttgatctcc agccatttct ccacacatac ctgcccattt tccttcagcg
    20821 tgtgctgcat caattacatt tttgatcaaa cgtaaaattg atgggttata tggttggtac
    20881 aagtaagaaa cgcgttcgtt catacggtct gctgccattg tgtattgaat caaatcgttc
    20941 gtacctacag agaagaaatc tacttctttt gcaaacttgt ctgctaagac tgctgctgct
    21001 ggaatctcga tcatgatgcc gacttggatc gtatcagata cttccttgcc ttcactgatc
    21061 aatttttgtt tttcttcttc aaagatcgct tttgctgcgc ggaattcttt gagtgtagct
    21121 accataggga acatgatacg taagttacca tgaacagatg cacgaagcaa tgcacgcatt
    21181 tgtgtacgga acatttcgtc gccttgttca gataaactga tacgcaatgc acgatatcct
    21241 aagaatggat tcatttcatg aggtaattgc agatatggta gttctttatc tccaccgatg
    21301 tccattgtac gaacaactac aggttttcct tccatacctt ctaatacagc tttgtaagct
    21361 tcatattggt catcttctgt tgggaagtct ggagaatcca tatacaggaa ttcagtacgg
    21421 tacagaccaa ctgcttctcc accattatta tggacaccaa ctagatcttt tggtgttccg
    21481 atgtttgcag ctaattcaaa atgcttgccg tcagctgtga cagtttctgc attttttaat
    21541 ttttcccatt ctgctttttg tgctgcgtaa tctgcacctg ctttttcaaa ttcagctttt
    21601 tgttcatctg taggatcgat gattacatcg ccttcgatcc cgttgacagc caaaatatcg
    21661 ccttctttga ctttagcagt aatttctttt gtaccaacga ttgctggaat ttctaaagaa
    21721 cgtgccatga tagcagaatg tgaagtacgt ccaccaatgt ctgttacaaa cgcttttaca
    21781 aaattgcgat ctaattgtgc tgtatcactt ggagtcaagt catgtgcaac aacgaccact
    21841 tcttcgttga tcattgatgg atttggtaaa gtaacgccta ataagtgcgc taaaatacgt
    21901 ttagcaacat cacgaatatc tgcagcacgc tcttgcatat aagcattatc ttccattgct
    21961 tcgaacatac cgatatacat atccgttact tcttttagcg ctgattcagc atttacgctg
    22021 ttgtctttaa tattttgctt gatttgacct accatttctg gatctgaaag aaccatcaaa
    22081 tgggcatcaa atacttgcgc ttctgcttca cctaagcttt gcgctgcttt ttcacgaatt
    22141 tgctgcaatt cttcagtaga tttagctaat gcagcatcca aacgagcttc ttcagccgca
    22201 gtatcttcta ctgttgcctt actgaatgtt aaatccggtt gaactagcag gtaagctttt
    22261 gcaacggcta caccgtcact agcggcaatc ccttttagca tttcaaccat tattcagata
    22321 agccttcttt cttcattgtt tcaacgattg ctgccattgc atcagcttcg tcagcacctt
    22381 ctgcagtaat agtaacatca gaaccttgac ctacacctaa agacataacg cccatgattg
    22441 atttcaagtt tactgattta cctttatact ctaagtttac atcagagtta aatttgcttg
    22501 ctgtttgtac taatagtgta gctggacgag cgtggatccc agtttctgct actacgtgaa
    22561 attctttctt ttccatatta atcggtctcc tttaagtgag tgttgttttt atgtgagggt
    22621 tttctttttt agtaattcag agaatcctaa caaatgccac cctttcatct gtaaagatta
    22681 acattttttt tcgttagata caacagtttt cattcaattt cagataaaag tattcaaatt
    22741 attccggatt ctttttcatc cttttcttca cattttataa caaacgtgtt tattttcttt
    22801 ttcttgtcgc tttctcttct cctgacgttt atcattcttt cctttgaccc tctttgtttt
    22861 catatgaaac gaattacttg cttccctttt tatttttagt ataataagag tcaaaggtca
    22921 ggaatggtca atagagattt caccttttct acctcataaa tctcttaaaa aactaggggt
    22981 tattctttta cctcatgaaa aacgagatgt aaaatatgga tgtacccatt tttgtgaaag
    23041 gacgtgaatg attcatgctt tgtcaaaact gcggcaagaa tgaggcaacg attcacttgt
    23101 atgccactgt caatggtcag cggacacaac tcgattactg tcagagctgt tatcaaaaaa
    23161 tcaaaaatca acaaaatgga ggtctgacta atatggccca aaatgatcca tttggatttg
    23221 gcagtttgga cgatcttttc cgttccatgt ctcgccaaat gcaacagcaa ggaaattatg
    23281 agcaaacacc accaacacaa tttggcggaa acaacaactt caatggtggt cagccacctc
    23341 aaggcaatac agaaggttta ttgggagaat acggaatcaa tatcacagaa caagcacgac
    23401 aaggtgatat cgatcctgtg atcggacgcg atgacgaaat caaacgagtc attgaaattt
    23461 taaatcgccg aaccaagaac aatcctgtct tgattggaga accaggtgtt ggtaaaacag
    23521 cagttgttga aggtttagca caaaaaatcg tagatggtga tgtaccgcaa aaattaatgg
    23581 acaaagaagt cattcgttta gatgtcgtat ctttagtcca aggtactggt atacgaggac
    23641 aatttgaaga acgtatgcaa aaattgattg aagagatccg tcaagcagaa aatgtgatcc
    23701 tattcataga tgaagttcac gaaatcgtcg gtgctggttc tgctggtgat ggaaatatgg
    23761 atgctggtaa tattttaaaa ccagctttag ctcgaggaga attgcaaatg gttggtgcta
    23821 ctaccttaaa tgagtaccgc atcatcgaaa aagacgctgc tttagaacgt cgtatgcaac
    23881 ctgtacgggt agatgaacca actgtcgaag aaacgatcag tattttgaaa ggtttgcaaa
    23941 aacgttatga agactatcat catgtaaaat atactgatga ggccatcgaa gcagccgcaa
    24001 ctttatccaa tcgttacgtc caagatcgct tcttgccgga taaagccatc gatttgctag
    24061 atgaaacagg ttctaagaaa aacttgacga tccaaatcgt tgatcctaaa acaatcgaga
    24121 aaaaactgca agaagcagaa gaacaaaaag ttttagcttc tcgagaagaa gatttcgaaa
    24181 aagcagctta ttatcgcgat cagatcaata aacttcaaaa aatgaaagaa cgtcagctca
    24241 ctgaagagga aacacctgtg attaccgaaa aagatatgga aaaaatcgtt gaacaacgga
    24301 caggtattcc agtgggtgaa ttaaaagaaa aagaacagac acaactgaaa aacttagcag
    24361 atgacttgaa agctcatgtc atcggacaag ataacgctgt agatcgagta gcgaaagcga
    24421 ttcgccgaaa ccgtgttgga ttaaacaaac agaatcgtcc aatcggctcc ttcttatttg
    24481 ttggaccaac tggtgtaggt aaaacagagc ttgcgaaaca acttgcctac gaactattcg
    24541 gttcacaaga ttcaatgatt cgtttcgata tgtctgaata tatggaaaaa catagtgtct
    24601 ctaaattgat tggttctcct ccaggttatg tcggatacga agaagctggt caattaacag
    24661 aaaaagtacg gagaaatcct tacagtcttg tcttgttaga tgaagtagaa aaagctcatc
    24721 cagatgtttt gcatatgttc ttgcaaatct tagatgatgg tcgattgaca gatgcacaag
    24781 gaagaacggt cagctttaaa gatacgatca tcattatgac aagtaatgcc ggaactggta
    24841 aagtagaagc aaacgttggt tttggtgcgg cacgcgaagg tgttactcgt tctgtattga
    24901 accaattaaa caactacttt acaccagaat ttctgaaccg ttttgacggg atcatcgaat
    24961 tcagtgcatt atcaaaagaa aacttgatga caatcgtcac actaatgcta gacgatgtca
    25021 accaaatgct ggcagcacag caacttcaca tcgaagttcc tacgaatgtc aaagaaaaat
    25081 tagttgactt aggctatgat ccatctatgg gtgctcgtcc tcttcgacgc acgatccaag
    25141 aacaaatcga agatggtatc gctgaatttt atcttgatca cccttctatc catgagttga
    25201 aagcaaaact ggataaagat ggaaaaatca tcgttacttc taaaccagaa cgtttggcta
    25261 aagaatcagc cgaagaaacg gctgaataaa tccagtttta tagcgtgaga cggtatttct
    25321 ttgagaaatg ccgtctcatt cttttttcag aaaagtcaaa atactgttca ttttataaaa
    25381 aagaaattat aataagagaa acaagcaatt aaaaatacat aaaagaggtg agcgccatga
    25441 aattggtaaa tgtaacaaac agccattccc gtcttgtatt aaaccagctt gaaaacactg
    25501 atgcccatct agtcaaggtc tacacagccg gaaataccac tattgtctac actgaagccc
    25561 ctgaacataa tgaaattctg ctgatcaatg acaaacgtaa aatccagccg aaagaaatcg
    25621 aagatgctaa aaattatttc ttagataaaa tcaaagacgc cgtttaccac gaggatgaaa
    25681 tcaaagtaat cgaattggca ggccttgttg aaatctcgat tccaaaacat gaattaggcg
    25741 ctacattagc cgtgtaaaca aattttgaac ataataatta aagagcgtgg gacaaaagta
    25801 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct
    25861 tcggaaataa gctaaaattc acgaaaattg cttttcaaat ttttgtgaat tccagcttat
    25921 ttcttggagt taaacgcttc tgtctcagcc tctttttctt tctagctttt tgatttttct
    25981 cttttctttt gtacttttct cttttcattt tcagttctac gcgtgtcttt atttttaact
    26041 ttttcctctt tatggatgat tgatcgtact ttttgtgcaa tttgctcgtt tttgtcccag
    26101 tcttttaaat ctaataatcg acataatcgt aacatctctt cttttttctg tttttttctt
    26161 tcacgtattt gccattctgt ttccatgtgt cacactctcc tttctttaat catatgtacg
    26221 caaaataatt tttatatttt tttgattaga catttctatg attttaatat agtaaaaaga
    26281 gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agagactgtt tttgtttggc
    26341 tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc aaacttttta
    26401 attttacatt aggatattta tccgcaaacc agcgttctgc aaattggttc tcgaataaga
    26461 acaatggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc atcttctcat
    26521 ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac atgattactt
    26581 cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc tgaccaacag
    26641 ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct ccttcttgaa
    26701 ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt gcagtcactt
    26761 tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt agttttcctt
    26821 cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg atatcgcctg
    26881 ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca ttgcttaatt
    26941 tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat gttccagaac
    27001 agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct tggatcttga
    27061 atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct tgtgttttgt
    27121 gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt acaccgaagt
    27181 tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt ttttcttgat
    27241 taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt tcatataagg
    27301 aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat tctactaatc
    27361 gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag atatcataaa
    27421 gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat tcgatatcta
    27481 atagctcttc taattcttct aacaattcca atggttcacg accatcacga tctagtttgt
    27541 tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat ttctttgtct
    27601 gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg gccatcaaag
    27661 tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata tttactcgtt
    27721 tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt tgtttctcga
    27781 tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg gtcccggctt
    27841 gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc ccagcatccg
    27901 gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat tctggattat
    27961 tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg cacagattgt
    28021 ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt gtagcagtct
    28081 ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc acccaagcca
    28141 aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg atagcttttt
    28201 cacaaccttt gtcaactact acaactgaat cagtatagca tggattcatc gaatgaaaaa
    28261 gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa aagacgtgtt
    28321 gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct tcttggctcg
    28381 tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc ttctgtttct
    28441 tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat ttcttcagaa
    28501 accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg aatcatgcct
    28561 aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag atcactgtca
    28621 aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc gtgttcatcg
    28681 attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc gacgatttct
    28741 tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc taataaaata
    28801 gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa gaccgtttca
    28861 ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa accgaattta
    28921 tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc tttgtcttcg
    28981 ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt ttcttccaca
    29041 gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc tcgggcaact
    29101 ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt caatacgcct
    29161 tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc atcatcaaaa
    29221 gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca gacgaagggc
    29281 ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac ttcttctgat
    29341 ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac ataggtcaaa
    29401 atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc taataccgga
    29461 gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt gaccaaagtg
    29521 attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt taaaagcttt
    29581 tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt gatcgaaaca
    29641 acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat caataataaa
    29701 atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt ctctccttat
    29761 aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg ttaaaatggt
    29821 atcacaaagc aattttcttt gtaataaaaa caagtgtact aaaactacct acgaggtcgt
    29881 agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga ttatatagga
    29941 aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa gcaatccggc
    30001 aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt tcagtgattt
    30061 cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta gcacaaccac
    30121 acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg caggtgctag
    30181 tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga ccccaaataa
    30241 gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac caacgaataa
    30301 acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt gccccaataa
    30361 ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg gcactagttt
    30421 ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta ctacggttgc
    30481 cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa ggaattgctg
    30541 aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat ctacttcctt
    30601 gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga tgaaatcagg
    30661 catattagat gccaatgagg aaatttgctg tactagactt gggatgacac taagtaaaat
    30721 ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata ttcttttgat
    30781 ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa agcctgcaat
    30841 cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa taaagtttat
    30901 ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc aaaacattag
    30961 tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta ctagtatata
    31021 ttatattcgt taaaaatt
//
LOCUS       DAXWLB010000012        30409 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXWLB010000012 DAXWLB010000000
VERSION     DAXWLB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.012"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(632..1135)
                     /locus_tag="ACGE4H_000446"
     CDS             complement(632..1135)
                     /locus_tag="ACGE4H_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8363888.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1494..2519
                     /locus_tag="ACGE4H_000447"
     CDS             1494..2519
                     /locus_tag="ACGE4H_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8363889.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2842..3721
                     /locus_tag="ACGE4H_000448"
                     /pseudo
     CDS             2842..3721
                     /locus_tag="ACGE4H_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            4197..4313
                     /locus_tag="ACGE4H_000449"
     CDS             4197..4313
                     /locus_tag="ACGE4H_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFF8363890.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5241..5579
                     /locus_tag="ACGE4H_000450"
     CDS             5241..5579
                     /locus_tag="ACGE4H_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363891.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5673..5903
                     /locus_tag="ACGE4H_000451"
     CDS             5673..5903
                     /locus_tag="ACGE4H_000451"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFF8363892.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6303..6674
                     /locus_tag="ACGE4H_000452"
     CDS             6303..6674
                     /locus_tag="ACGE4H_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFF8363893.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6948..7640)
                     /locus_tag="ACGE4H_000453"
     CDS             complement(6948..7640)
                     /locus_tag="ACGE4H_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8363894.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7643..8428)
                     /locus_tag="ACGE4H_000454"
     CDS             complement(7643..8428)
                     /locus_tag="ACGE4H_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8363895.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8665..10065
                     /gene="lacG"
                     /locus_tag="ACGE4H_000455"
     CDS             8665..10065
                     /gene="lacG"
                     /locus_tag="ACGE4H_000455"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8363896.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10090..10407
                     /locus_tag="ACGE4H_000456"
     CDS             10090..10407
                     /locus_tag="ACGE4H_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8363897.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10420..12093
                     /locus_tag="ACGE4H_000457"
     CDS             10420..12093
                     /locus_tag="ACGE4H_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFF8363898.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12107..13738
                     /locus_tag="ACGE4H_000458"
     CDS             12107..13738
                     /locus_tag="ACGE4H_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFF8363899.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14229..15794)
                     /gene="guaA"
                     /locus_tag="ACGE4H_000459"
     CDS             complement(14229..15794)
                     /gene="guaA"
                     /locus_tag="ACGE4H_000459"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFF8363900.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15853..16776)
                     /gene="coaA"
                     /locus_tag="ACGE4H_000460"
     CDS             complement(15853..16776)
                     /gene="coaA"
                     /locus_tag="ACGE4H_000460"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFF8363901.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16972..17577
                     /locus_tag="ACGE4H_000461"
     CDS             16972..17577
                     /locus_tag="ACGE4H_000461"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8363902.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17885..18901
                     /locus_tag="ACGE4H_000462"
     CDS             17885..18901
                     /locus_tag="ACGE4H_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8363903.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            19042..20343
                     /locus_tag="ACGE4H_000463"
     CDS             19042..20343
                     /locus_tag="ACGE4H_000463"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFF8363904.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20389..21051
                     /gene="deoC"
                     /locus_tag="ACGE4H_000464"
     CDS             20389..21051
                     /gene="deoC"
                     /locus_tag="ACGE4H_000464"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFF8363905.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            21072..21470
                     /locus_tag="ACGE4H_000465"
     CDS             21072..21470
                     /locus_tag="ACGE4H_000465"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFF8363906.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21536..22618
                     /locus_tag="ACGE4H_000466"
     CDS             21536..22618
                     /locus_tag="ACGE4H_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8363907.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22830..24392
                     /locus_tag="ACGE4H_000467"
     CDS             22830..24392
                     /locus_tag="ACGE4H_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363908.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24385..25518
                     /locus_tag="ACGE4H_000468"
     CDS             24385..25518
                     /locus_tag="ACGE4H_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363909.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25518..26474
                     /locus_tag="ACGE4H_000469"
     CDS             25518..26474
                     /locus_tag="ACGE4H_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363910.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26573..27739
                     /gene="deoB"
                     /locus_tag="ACGE4H_000470"
     CDS             26573..27739
                     /gene="deoB"
                     /locus_tag="ACGE4H_000470"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFF8363911.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27763..28581
                     /locus_tag="ACGE4H_000471"
     CDS             27763..28581
                     /locus_tag="ACGE4H_000471"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8363912.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28598..29305
                     /gene="deoD"
                     /locus_tag="ACGE4H_000472"
     CDS             28598..29305
                     /gene="deoD"
                     /locus_tag="ACGE4H_000472"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFF8363913.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29603..30289)
                     /gene="gpmA"
                     /locus_tag="ACGE4H_000473"
     CDS             complement(29603..30289)
                     /gene="gpmA"
                     /locus_tag="ACGE4H_000473"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8363914.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT         9537 a         5293 c         6519 g         9060 t
ORIGIN      
        1 caaagaaggg cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt
       61 aacgtgtttt caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc
      121 attttacgct tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca
      181 gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta
      241 ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac
      301 catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct
      361 gagtagggta taaattaaga cggaattcct ttctagattg agatattgac tagcaagaat
      421 gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata
      481 gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa
      541 aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg
      601 tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa
      661 ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt
      721 ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc
      781 ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt
      841 gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa
      901 gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag
      961 tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga
     1021 cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca
     1081 accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca
     1141 tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa
     1201 gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg
     1261 gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc
     1321 aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata
     1381 ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt
     1441 ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat agattgttca
     1501 agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc
     1561 tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt
     1621 tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc
     1681 tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat
     1741 cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat
     1801 tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg atgctttctg
     1861 ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt
     1921 ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc
     1981 ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga
     2041 tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct
     2101 tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa
     2161 acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat
     2221 gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca
     2281 cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt
     2341 ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt
     2401 tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt
     2461 gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga
     2521 aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca
     2581 aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga
     2641 actttcaaaa aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa
     2701 taaactttcc tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat
     2761 agaaagttta agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc
     2821 ggataatcag tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag
     2881 aaaataagca atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata
     2941 gagcaaatga ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat
     3001 gaactaatgg atcctaataa agccaaaggc gactgcaaag aaaatagaca aagtctatga
     3061 agaggaacct tttgcagcat tagctattta tctatatgca gcttctcaaa gttggaaaaa
     3121 aatttatcaa ccattgaaga ttccaagaac tatctatttg gcaacaatga atgcttttac
     3181 tagatttatt catgaacatt tccaagtaac aggaagctat cgctttgacc gtggtttttg
     3241 gatttggcat tacactagtg gcttgatctt tcggattggt gaattggagt acgaaatgac
     3301 atattttccg aaagggtaca cggatctaag attagaagga aagcagtgcc tttctcttca
     3361 tattccttcc gatgcagatt tatctcagaa taagataaga gatagttacg aaagtgccat
     3421 tcgttttttt gaactttatt ttcctgatta ccattaccaa gtgatgtaca cagatacttg
     3481 gttactatca cccaatctta ctaaatggtt gaaaaaagag tcaaaaatct gtctttttgc
     3541 agccgattat cgattattat cagttgatga acaggacgat agtggagtcc cttggatctt
     3601 tggacgagta gatgcccaaa tacatgatta tccagaaagt acgagtttac agcgacaagc
     3661 aaaagagcaa ttgcttgcag gcgagcacat tggctctggc ttaggtattc ttacttcata
     3721 agtttagtac ttcgaaatag tttaaaagag cgtgagacaa aagtaaagaa cacttttgtc
     3781 tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctga
     3841 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
     3901 gcttctgtcc cagcctcttt tttagtgttc tatcgaacac acagtacgtt caataggtaa
     3961 actagctata acaatagata ttaaagttaa cggacaagag ggcctttttt aggtagctaa
     4021 aacgtaataa tgaaaattta tttccagaga taggagtcaa tcagctagtc tgatcgactg
     4081 ttttaacaag ttgactttca ttttaacttt tgataccatt taaaacacaa atcgtaattg
     4141 tttcgatttg tgttttttag catgactaca atcaaagaag aaagtgggtc gtccatatgg
     4201 aaaaaacaga tatctcaagt gcataccgcc gcttgaaaag cccaaatatc aaaacacgaa
     4261 aacgtgcgct gaagatcatc aaagatgtga aacggaatag tgggaaaagg taagaagaag
     4321 gaaacttaat taaaaaaata gccgaaaaac gtttttctgc tattttcaat aatcggtttt
     4381 tgtgtaaggt ttccgaaaga actgctgttg attttccatt tttaacatat gataaattgg
     4441 tagaatcttt cttttcaaat tgataccatt ccctaattcc agttatatag ttaactacag
     4501 aaccacaaag cacgtacgtt tataaaagta ttatctgtag ttatttttca attccctttc
     4561 aacctcttat tttgaccagt agtattgatc actttacctt ttaacacctt tgttcgtttt
     4621 tgaaacattt ttagtcaaga atggaaacgt aactacataa tcaaaggttt tgcgaaatat
     4681 ttcatgaaat atttcatgta gaagcgagca atacggttgc aaataaccat tatcgtttaa
     4741 taagcagtta cgaaagctcc caatatctct aacgttcagc tcactccatt tcttttcttc
     4801 aaaaaaatca gtgattcgta tctctttcta tttgtcgaaa aagttaactt taaaaagtaa
     4861 cgccgaaaaa atatgcaaaa aaataaaaaa gcagttacta ttgctagaat aacatcaagg
     4921 aataataagt tttggttttc agataatagt cgataagttg tgtattggaa ttttagatac
     4981 agtaataaag tatttttcat gaaattttgt gagaatcact attacaagta tctttaataa
     5041 aattaatggt taaggaggaa cttaaagtag aatgtaaatg aatagttgta gtcagtcgat
     5101 tgtgatacta gactcaaagt tgcaggaata agtttttcaa tttgtgattg gaagataaaa
     5161 aatgaaaaag aagggctttt ttttatactt tgttgattgt agtgtttatt ttccctctgt
     5221 ttagccaagt aacattttct atgactcaac cagtttcaag tgaggaacat gataaccttt
     5281 ttcgtgctac agaagagttt aagaaatatc aggaaaatat tgttccggag actccattag
     5341 ctagtgagcc aattactgat attgatggaa accaaatagg atggtatata cagtacgaag
     5401 ttatggtggt aaataatgac tcaatagtaa aaaatacagt aggcttagag ctaacaccga
     5461 ttgaaaaggt gagttcattg ttaacgtata tttatactga tgaaagtgac gagactgaga
     5521 tcattttaac ggattactca aaaattgcta ctgataaaaa gtatatattg tggatgtaga
     5581 cactagtgaa atactttatg atatagaaat tgatgaaaat ttactagatg tagcacaaga
     5641 aatcgatcat caaaagcagg aagctattcg atatgctggt agaaaaggga aaagaaaata
     5701 tttattacgt gaatgtagca aaagttagag aagatgaaaa tgagtggaaa gaattcaaga
     5761 gtcgctactc aattaactcg actccgacat ttacagttta cagagaagga tcgatagaaa
     5821 aaactgtttt ttggactaaa gaaagtggaa tgtctttagc tgaagtagaa gagtttttgg
     5881 attatgttag tatgcaacag taagggagtt cttattatga aaaattttga cacatattat
     5941 gttattgttt tttcgatatc tttgctgtta atattactaa ctttaatgca gtataaagag
     6001 tatccagagt attttatttt aacttttgta tatttagtaa gtagctacac acttctatat
     6061 aaattcagaa gttcttctag agtttctaaa gtttgtttct taattagctt attgattcta
     6121 taatattttt gatttatata ggaaattaaa aatattatag aatcaacact cagagaacgg
     6181 gttggttttt aatagattta ataagtactg ataatgaaat ttctcctggt tgggaaaacg
     6241 aagttggtta ccctgtagaa gaattttttc aagattactt tgcggagtat gccgatagga
     6301 taatgagaga ttacgtaaca gataaaactc aagaagtcat caatcaatcc cagtcattaa
     6361 atgaattatc tgataaggcg aaagaagaag cagttgccat tatttcttca gggattagtg
     6421 actatattga tatgagtggt gaaatcaatt atggcgataa agacaaaatt gtacgcatga
     6481 taaaagcttt tatcaaaact tttgatgtga aggcagatct taagtttaat gatacttacc
     6541 tagtaggaaa attaattaat atccttggga cagagaaaga tacggaaacg tttatttcct
     6601 ctttgtctat gttgtttaca gataagcatt tttcaactca gcttggtgga gaggaactag
     6661 tggaaatctt ttaaccatta cgcccagcat taataaaagt atatcaaaaa agtactcctg
     6721 atgattttta agattggttt gagaaataat gtgaaaacac tatttttaat gttaattata
     6781 gatagagatg aacgcgtcat atggttaaaa gaatgaaaaa gtcaaagata cactgctata
     6841 tttcactaag gatatagcag tgtatctttc atgtcgtttc actggcagtt ttttataaaa
     6901 ggaggtgtgc atttcagttg cttttctttg gtagttagtt gaatcgttta agaattaacg
     6961 gatgtggcag acaggtgctc tgtccaaaaa ccagtaaaat ctttgatggt tccatcttta
     7021 tttagctgtg cctgttcaaa tactgttttg tttttaccga atggatcaat tgcaataatt
     7081 gttccgatcc ccgcacgttt ggcagcaagt aatcctgagt aagcatcttc gataacaaga
     7141 caatcagccg gagctaagct cagtttgtct gccgctttaa gaaaaatatc cggttgcggt
     7201 ttcccaggga aagagccgtc gtcatagaca atcttgtccc agtcaaacca gcgggctaaa
     7261 tcgaaaatat caaaataaaa agcaacattc tctttgacag tagccgtggc aatcgttaat
     7321 ggaatctgtt ggtcagtcag ctggtcaaga gtcgcttcaa gcccttttgt caactgaagc
     7381 tcgttttcat tttgcaagca aagcatacga taatagcttt ctttttcttc tgacaatacg
     7441 gcgatctcag aggcggtcaa atgctctgaa atgaaatgtt tcaaaatctc cgtattcgtc
     7501 cgaccatgaa tattttttaa aatatcctcg tctgataaac catcttttgt atgatcatgt
     7561 atcatatgca gccaagcagc ttcatgcaga tgagagtcca aaaacattgt tccgttgaaa
     7621 tcaaatataa tccctttcat agttacgctc ctatagtggt attttcggtt gaatcagcaa
     7681 tttgcagatc gacatattgt gcatagtgct cttgaacctc ccgcgagatc tggcgatccg
     7741 taatgagcag atcgaaatca tgcaaattat aaaacacata aaaatcatct cgattgaatt
     7801 ttttattatc aacgagtaga tatttcttac gggaattatt tagagcgatc tgctgtgctt
     7861 ctccttcttc ggtactgtaa gtagagatcg cttcgttgtg aacgccattt gaactgataa
     7921 atgcttttgt aaactttagt cttcttaaat tgtcgttggt gatcggtccg acaaatgcgc
     7981 cggtattttt ccggaaatca ccgccaatca gtagaatttc tggtccatct gcttgagcaa
     8041 gaatatcaaa aactggataa ttatttgtaa tgatccgaat tttcttgttc agtaaatgcg
     8101 tcgccaatag ttcgattgtt gtacctggtc ctaagaaaat cgtttctccc tcgtgaatca
     8161 aggaggctgc tgtggcagca atttcttttt tttcttgaat ctgcagagtg gctttttcag
     8221 tgtgagaaag ttcttgatct aaagtgtagg aaagactttg agcccctcca tggacacgca
     8281 gcagcttgcc cgatttttct aattcatcta aatctctgcg aacggtcata tcagaaacat
     8341 ctaattgatt gatgatttca ttaacggtaa ggattccttt tttattgacc atttctacga
     8401 tagtaagcaa tctttctttt ttcaacattg tcttcacctc taatttaagg ataatctatc
     8461 atataaacgg acaacagtca atctaattcg aacaaaagta aacatttagg gtggttttta
     8521 tgtttgttac aatagatttt attgctgttt tctggttttt atgattattt gtgtttgttt
     8581 ttgttgacaa atgttttgga tgcgtttaca atgcaatcag ttagaaagag cgaaaactga
     8641 ttatagattt gaggagagat gtacatgctt aaattaccag aagattttat ttttggcgga
     8701 gccacagcag cctatcaggt tgaaggagcc actaaggaag gtggcaaagg cgctgttgct
     8761 tgggatgact ttttagagga acaagggcga ttcagtcccg atccggcaag tgatttttat
     8821 catcaatatg caaaggacat tgaattgtgt gaacgttttg gtgtaaatgg tctaagatta
     8881 tccattgctt ggagccgcat ttttcctgat ggagctggga aaccaaatcc agaaggaatc
     8941 gctttttatc atcgtgtgtt cgaagaatgt aagaaaagaa acgtaacgcc gtttgtgacg
     9001 ttgcatcatt ttgatacccc taaacgtttg tttgaccatg gagatttttt gaatcgagaa
     9061 acaatagaag catttgtttc ctatgccatt ttttgttttc atgaattcaa agaagtcaag
     9121 gtgtggagta cctttaatga aatctatcca gttgcaacca atcaatattt gttaggtgtt
     9181 ttcccaccag gaatcaaata tgattttaca aagatcgtcg cctgtcttca taatatgatg
     9241 gttgctcatg cccgtgtagt gaactatttc aaagaaaacg aacttccggg tgaaattggt
     9301 gtagttcact cattagagac gaaatatgcg gcaacagacg cgccggaaga taagcatgct
     9361 gcctttttag acgatgcatt gtcgatccgt ttcttattag atgctaccta tttaggctat
     9421 tattcaactg aaaccttgac tgctcttgat gagatatgtg aagcaaatca ggccagctat
     9481 cattttcctg aagaagattt cgttgaattg aaaaaagcat ccacgcgtaa tgactactta
     9541 gggatcaatc actaccaatg ccattttgtc aaagcttacg atggagaaaa tgcaatccat
     9601 cataacggta cgggtgaaaa agggacctct gtttataaag tgaagggaat cggagaacgt
     9661 atttataaag aaggaattcc tcgaactgat tgggactggc tgatttatcc agaaggtcta
     9721 tatgacttgt tgctgcgaat taaaagcgat tacccacatt acaacaaaat ctatatcact
     9781 gagaacggga tggggtataa agatcagttt gaagatggca ttattatgga tcagccgaga
     9841 atcgactatt tgagagtcta tttggaatcc ttaagcaagg cgataacagc cggggtaaat
     9901 gtgaaggggt attttttgtg gtcgcttatg gatcttttct catggacaaa tgggtataac
     9961 aaaaggtatg gcttgtttta tgttgatttt gaaacccaga agcgttaccc aaaagaatca
    10021 gcttactggt acaagttggt cagtgaaacc aaaacgatta tttaataagt gataatggag
    10081 gaagaaaaga tgaataaaga ggaattacaa atgcttggtt ttgagatcgt tgcatattca
    10141 ggcgacgctc gcagtacatt attgaaatta ttgaaagaag taagacaagg aaactttgaa
    10201 catgttgatt cggcgttgaa agaagccgac gaaaatttaa cccttgcaca taattcgcaa
    10261 acaaaaattt tagcggaaga agcatctggg aaagagatgg agatgggatt catttttatc
    10321 catgggcagg atcatttaat gaccacttta ttgctgagag atctgattca agactttatt
    10381 gtactgtaca gacagaatca aggataggag aagaacgcta tggatggaat cattaaacaa
    10441 atcgaaaaag ggaaaccatt ttttgaaaaa atctcacgta atagttattt aggagccgtt
    10501 cgtgacggat ttttaacagc gatgccagct attttatttt caagcatttt cattttgatt
    10561 gctgcggttc ctgagatttt tggttaccaa tggcctgaat ctgtctccac atggctatgg
    10621 aaagtctata attattcgat gggtgttgta gggttactcg tttcagcaac cactgctcgc
    10681 tgcttggctg attcgatgaa tcgtcgctta ccgaaaaaca aagtaatcaa caccacctct
    10741 gtgatgctgg cctctgtggt aagtttcctg ttgttaagcg tctctccgat tgaaggtgga
    10801 ttttcaacgg aatatatggg gacaaaggga ctactggctt cctttgtcgc tgcattttta
    10861 acagttaatt tatataagtt ttgtgtgctg aaagatatta ccattaaaat gcctaaagaa
    10921 gttcctggaa cgatttcgca aaccttccga gatatttttc ctttctcttt ttcagtattt
    10981 gcggccgttt taattgattt aggcattcga caaatcttcc aaatctcatt tgcagaagca
    11041 atcattacgg tgctgcagcc gctatttaca gcagctgacg ggtacttagg aatcgctatt
    11101 atctggggag cgatggcgct gttttggttt gtcggtgttc atggaccgtc gatcgtggag
    11161 ccggcaattg cggcaattat ctatgccaat gtggaaacca atctgcaatt gttcaattcc
    11221 ggtgaacaag cttctaaagt actgacggtg ggtttaggga attttgtagg aactatggga
    11281 ggaactgggg caacattggt tgttcctttt atcttcctac tctttgctaa gtctaagcag
    11341 ttgaaggcag ttgggaaagc ttcctttatt ccagtaagtt tcgctgtcaa cgaaccattg
    11401 ctatttgcgg cacccatgat tttgaaccct tattttttta ttccattttt gttaacacct
    11461 gttgtaaacg tttgcctgtt caagtttttt gtcgatgtat tgggcatgaa cagctttatg
    11521 tacgttttgc cttgggcaac tccagcaccg atcggtttgg ttctaggaac gggcatgggt
    11581 atcttagctt ttgttctagc agcggttcta atcgtcgttg acttcctgat ctatctacca
    11641 ttttgcaaag catacgatgc tgtcttagta gagaaagaag cacaaaaagc agaggtgatc
    11701 gcaaccgagg aaggtgcttc tgccgatgta cgtgaggaag tacctgtggc agcgagtcaa
    11761 gttatcagcg atgcaaataa agaaatcaat gtgttggtac tttgtgctgg cgcagggaca
    11821 agtgcgatgt tagctaatgc tttaaaaaaa ggtgcatctg aatcgaaagc accgatcagt
    11881 gcaatggcgg gtgcttatgg ttctcattac gaaatgctgc aggattttga tatggtggtc
    11941 ttagcaccgc aagtcaattc ttactatgat gatatcaaag aagacaccga tcgcttagga
    12001 atcaagctgg ttgcgacaaa aggagcagaa tatatcggat taacgagaga tcctgaaaaa
    12061 gctgttcagt ttattctgaa ccaatttcaa taaaggagga gccataatga aaatgggaat
    12121 ttggttgcgt aaagctgttt tatttgcagc tgtgttaggg gctttggggg ctgtttcaaa
    12181 aactgttgaa gcatctgcca gcgaatttat tcgcggagca gatatttcga tcctgtcaga
    12241 tatggaaaaa tctggtgcaa aatattatga agacgatatt tcaaaagatg ccttagagat
    12301 tttaaaaaat aatggtgtca actatgttcg actgcgtttg tggcaggatc cttataatag
    12361 taatggagaa agttatggag cagggacaaa tgatctggaa acgacgattg accttgcaaa
    12421 acgagcaaaa aatctgggac ttaaggtgct gctggatttt cactacagtg atttttgggt
    12481 tgatcctggg aaacagaatc ttcctaaagc atggcaaggt ctgacctttg aagaaatgaa
    12541 tacggcattg tacgactata caaagaacgt tctgtcggaa atgaagcagc tggacgttta
    12601 tcctgatatg gttcagatcg gcaatgaact gaatagcgga atgctctggc cttatggcaa
    12661 gtcttgggga gaaggcgggg gagaatttga tcgattggct gcttttttga agtctggtat
    12721 ccaagctgtt cgagacactc aacctaaaac aactccagta atgctccact tagctgatgg
    12781 tggcgataca ggagcattta cttggtggtt tgatgaaatc acgagtcgag gtgtgtcatt
    12841 tgatttaatt ggagtgtcgt attatccgta ttggcacggt tcaatgaatg aacttcaaac
    12901 caatatgaat gcgatcagcc aacggtatgg taaagaagtg atcgtagttg aaacagctta
    12961 tgggcataca acagcgaatg ccgatactat gccaaatgct tttggtgaag ccgaagcagc
    13021 tgcgggggga tatgaaccaa caccgacagg acaagcagag tatttgctgg atcttgccga
    13081 tcggattcag gcagtaccga ataatcgcgg ggcgggcttc ttctattggg aacctctttg
    13141 gtacaacgga aatgtgagtt gggcaacaca agcaggtatg aattatctca gtgttcaatc
    13201 cactatggga aacgaatggg acaaccaagc gatgtttgat tttgctggaa acgccttgcc
    13261 aagcttgcgt gcgtttaaac aagcaggggc gcaaataaat ctagttaaaa atgcaagttt
    13321 cgaaagcgat ggaccaacga ccagtccttc caattggcaa acctggtttc aacaaggaac
    13381 cgctaagggt acgatcaaaa cggaagcggg cacttttttt ggggactaca aactaacttt
    13441 ttggcaggat caagcatacg aggcatcagc ttatcaaaat gttagtggca taagcaaagg
    13501 aacttacaca ttgagtgcct atgtaatgtc tgggggagag tttgatacta atcagttata
    13561 tatcaaaaat tatggtggag cggaactaaa tactgcggtt gttaaaacaa gtgaatggac
    13621 aaaaattaca atcaaagaca ttccaattac aacaggtgtt tgtgaaattg gggtttatag
    13681 caaggcttca ggaaatgctt ggacgaacgt tgattttgtt tcattggtga aacaatagac
    13741 tgcggatcgt ttcgattcaa gatcggaggt tcccttcagc cacatcgtta gcgtagaagg
    13801 caggaatcga acctgcacct cgcctttgcc aaaaataacc agaaacgatt tccagtcatt
    13861 tttaccaaaa cgagtaaagc accagacttc atctacactt tgtattatgc taaagagagt
    13921 tcaacgattt cgtaagaagc aatgaaaaaa ttgcaaaaag ttgtgcagat aacataaagt
    13981 ttgtttaaga aagggtaacc ttagaattcc gtcgtgaata tgatttgagc acttagatgg
    14041 atagaaaaaa gttctcggaa aacagttcgt ttttcgagaa cttcttttta tgataagaat
    14101 gaaacaaatt aacttatatt atagtctctt ttggtaggga accatcttat ttttaatgaa
    14161 aatcaagcat tttagctatt gaagaatgtt gatttatagg gattccccct ccatattatg
    14221 ttggctcttt attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
    14281 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
    14341 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
    14401 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
    14461 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
    14521 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
    14581 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
    14641 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
    14701 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
    14761 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
    14821 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
    14881 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
    14941 ttgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
    15001 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
    15061 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
    15121 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
    15181 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
    15241 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
    15301 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
    15361 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
    15421 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
    15481 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
    15541 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
    15601 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
    15661 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
    15721 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
    15781 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
    15841 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
    15901 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
    15961 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
    16021 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
    16081 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
    16141 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
    16201 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
    16261 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
    16321 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
    16381 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
    16441 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
    16501 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
    16561 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
    16621 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
    16681 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
    16741 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
    16801 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
    16861 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
    16921 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
    16981 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
    17041 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
    17101 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
    17161 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
    17221 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
    17281 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
    17341 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
    17401 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
    17461 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
    17521 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
    17581 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
    17641 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
    17701 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
    17761 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
    17821 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
    17881 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
    17941 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
    18001 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
    18061 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
    18121 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
    18181 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
    18241 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
    18301 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
    18361 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
    18421 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
    18481 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
    18541 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
    18601 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
    18661 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
    18721 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
    18781 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
    18841 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
    18901 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
    18961 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
    19021 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
    19081 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
    19141 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
    19201 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
    19261 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
    19321 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
    19381 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
    19441 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
    19501 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
    19561 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
    19621 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
    19681 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
    19741 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
    19801 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
    19861 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
    19921 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
    19981 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
    20041 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
    20101 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
    20161 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
    20221 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
    20281 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
    20341 taaaggaaaa aagcggactt tgccgtttta aagaaaaggg gaaaagaaat ggaactaaac
    20401 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
    20461 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
    20521 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
    20581 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
    20641 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
    20701 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
    20761 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
    20821 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
    20881 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
    20941 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
    21001 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
    21061 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
    21121 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
    21181 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
    21241 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
    21301 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
    21361 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
    21421 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
    21481 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
    21541 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
    21601 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
    21661 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
    21721 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
    21781 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
    21841 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
    21901 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
    21961 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
    22021 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
    22081 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
    22141 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
    22201 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
    22261 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
    22321 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
    22381 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
    22441 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
    22501 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
    22561 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
    22621 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
    22681 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
    22741 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
    22801 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
    22861 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
    22921 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
    22981 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
    23041 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
    23101 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
    23161 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
    23221 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
    23281 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
    23341 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
    23401 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
    23461 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
    23521 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
    23581 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
    23641 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
    23701 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
    23761 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
    23821 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
    23881 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
    23941 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
    24001 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
    24061 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
    24121 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    24181 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    24241 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    24301 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    24361 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    24421 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    24481 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    24541 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    24601 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    24661 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    24721 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    24781 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    24841 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    24901 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    24961 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    25021 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    25081 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    25141 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    25201 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    25261 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    25321 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    25381 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    25441 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    25501 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    25561 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    25621 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    25681 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    25741 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    25801 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    25861 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    25921 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    25981 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    26041 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    26101 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    26161 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    26221 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    26281 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    26341 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    26401 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    26461 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    26521 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    26581 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    26641 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    26701 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    26761 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    26821 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    26881 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    26941 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    27001 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    27061 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    27121 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    27181 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    27241 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    27301 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    27361 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    27421 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    27481 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    27541 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    27601 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    27661 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    27721 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    27781 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    27841 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    27901 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    27961 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    28021 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    28081 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    28141 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    28201 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    28261 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    28321 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    28381 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    28441 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    28501 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    28561 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    28621 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    28681 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    28741 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    28801 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    28861 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    28921 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    28981 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    29041 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    29101 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    29161 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    29221 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    29281 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    29341 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    29401 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    29461 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    29521 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    29581 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    29641 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    29701 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    29761 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    29821 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    29881 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    29941 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    30001 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    30061 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    30121 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    30181 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    30241 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    30301 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    30361 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagt
//
LOCUS       DAXWLB010000013        30404 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXWLB010000013 DAXWLB010000000
VERSION     DAXWLB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.013"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(56..184)
                     /locus_tag="ACGE4H_000474"
     CDS             complement(56..184)
                     /locus_tag="ACGE4H_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8363915.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(187..1560)
                     /gene="rlmD"
                     /locus_tag="ACGE4H_000475"
     CDS             complement(187..1560)
                     /gene="rlmD"
                     /locus_tag="ACGE4H_000475"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8363916.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1671..2726)
                     /locus_tag="ACGE4H_000476"
     CDS             complement(1671..2726)
                     /locus_tag="ACGE4H_000476"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8363917.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2736..4166)
                     /gene="gatB"
                     /locus_tag="ACGE4H_000477"
     CDS             complement(2736..4166)
                     /gene="gatB"
                     /locus_tag="ACGE4H_000477"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFF8363918.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4163..5635)
                     /gene="gatA"
                     /locus_tag="ACGE4H_000478"
     CDS             complement(4163..5635)
                     /gene="gatA"
                     /locus_tag="ACGE4H_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFF8363919.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5635..5940)
                     /gene="gatC"
                     /locus_tag="ACGE4H_000479"
     CDS             complement(5635..5940)
                     /gene="gatC"
                     /locus_tag="ACGE4H_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFF8363920.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6046..8082)
                     /gene="ligA"
                     /locus_tag="ACGE4H_000480"
     CDS             complement(6046..8082)
                     /gene="ligA"
                     /locus_tag="ACGE4H_000480"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFF8363921.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8099..10333)
                     /gene="pcrA"
                     /locus_tag="ACGE4H_000481"
     CDS             complement(8099..10333)
                     /gene="pcrA"
                     /locus_tag="ACGE4H_000481"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFF8363922.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10488..12047
                     /locus_tag="ACGE4H_000482"
     CDS             10488..12047
                     /locus_tag="ACGE4H_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFF8363923.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12158..14089)
                     /locus_tag="ACGE4H_000483"
     CDS             complement(12158..14089)
                     /locus_tag="ACGE4H_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8363924.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14107..15021)
                     /gene="pfkB"
                     /locus_tag="ACGE4H_000484"
     CDS             complement(14107..15021)
                     /gene="pfkB"
                     /locus_tag="ACGE4H_000484"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8363925.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15018..15770)
                     /locus_tag="ACGE4H_000485"
     CDS             complement(15018..15770)
                     /locus_tag="ACGE4H_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8363926.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15929..16138)
                     /locus_tag="ACGE4H_000486"
     CDS             complement(15929..16138)
                     /locus_tag="ACGE4H_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363927.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16149..17477)
                     /locus_tag="ACGE4H_000487"
     CDS             complement(16149..17477)
                     /locus_tag="ACGE4H_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363928.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17613..17768
                     /locus_tag="ACGE4H_000488"
     CDS             17613..17768
                     /locus_tag="ACGE4H_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFF8363929.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17842..18429)
                     /gene="yihA"
                     /locus_tag="ACGE4H_000489"
     CDS             complement(17842..18429)
                     /gene="yihA"
                     /locus_tag="ACGE4H_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFF8363930.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18512..19762)
                     /gene="clpX"
                     /locus_tag="ACGE4H_000490"
     CDS             complement(18512..19762)
                     /gene="clpX"
                     /locus_tag="ACGE4H_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFF8363931.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20009..21298)
                     /gene="tig"
                     /locus_tag="ACGE4H_000491"
     CDS             complement(20009..21298)
                     /gene="tig"
                     /locus_tag="ACGE4H_000491"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFF8363932.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21452..22384)
                     /locus_tag="ACGE4H_000492"
     CDS             complement(21452..22384)
                     /locus_tag="ACGE4H_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363933.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22541..23200)
                     /locus_tag="ACGE4H_000493"
     CDS             complement(22541..23200)
                     /locus_tag="ACGE4H_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFF8363934.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23203..24228)
                     /gene="sppA"
                     /locus_tag="ACGE4H_000494"
     CDS             complement(23203..24228)
                     /gene="sppA"
                     /locus_tag="ACGE4H_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFF8363935.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24241..24531)
                     /locus_tag="ACGE4H_000495"
     CDS             complement(24241..24531)
                     /locus_tag="ACGE4H_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363936.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24941..26746
                     /gene="glmS"
                     /locus_tag="ACGE4H_000496"
     CDS             24941..26746
                     /gene="glmS"
                     /locus_tag="ACGE4H_000496"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFF8363937.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26818..28173)
                     /gene="glmM"
                     /locus_tag="ACGE4H_000497"
     CDS             complement(26818..28173)
                     /gene="glmM"
                     /locus_tag="ACGE4H_000497"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFF8363938.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28197..29366)
                     /locus_tag="ACGE4H_000498"
     CDS             complement(28197..29366)
                     /locus_tag="ACGE4H_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFF8363939.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29363..30250)
                     /gene="cdaA"
                     /locus_tag="ACGE4H_000499"
     CDS             complement(29363..30250)
                     /gene="cdaA"
                     /locus_tag="ACGE4H_000499"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFF8363940.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         8178 a         6586 c         5439 g        10201 t
ORIGIN      
        1 aaagactaag aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt
       61 tattaaagat tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt
      121 gtttggatcg gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt
      181 ccatttttat tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa
      241 aagatctaca ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc
      301 tcttgcaaaa gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat
      361 tgctgattca ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga
      421 tggtttgatc cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata
      481 atgaacattt tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg
      541 aataacttcc atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc
      601 tattcctgaa taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc
      661 tgtttggtac agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat
      721 tcgataggtt tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc
      781 tttgtctcct aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac
      841 ttctggcaat tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt
      901 acgagtaact aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg
      961 acgaagctgt ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat
     1021 atctctgact actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat
     1081 agggactaga tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg
     1141 gcgaacaggg atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg
     1201 aagtacagga acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg
     1261 tttgaagaca agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag
     1321 taatgcattg ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt
     1381 tccatatcca aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag
     1441 tgcattttcg ataaataatg gataaccatc gatttttgct acccccattc cttcataact
     1501 caagtcgacg atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat
     1561 attttgctcc tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt
     1621 acacaactaa aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt
     1681 tgattaggtg tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt
     1741 tcgacttctt ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg
     1801 attgcttcat caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt
     1861 tggaaatgca tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga
     1921 ttgatatcat cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga
     1981 tcatcgacat gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata
     2041 tttgctgtct tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg
     2101 atctgttcga atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg
     2161 atatattccc cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc
     2221 tctgctcctt tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact
     2281 tcataagtca attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca
     2341 gaaaggccga tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta
     2401 atattatctc taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg
     2461 atggccattt taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg
     2521 ccatcgccac cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca
     2581 ttcttggctg aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct
     2641 tctaatgcct gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg
     2701 ttataaatta cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta
     2761 agtagcttat tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg
     2821 actaggaagc caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg
     2881 ttatccaaaa tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa
     2941 ccattttttt caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact
     3001 tttttggcga tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag
     3061 ttctcaggtg tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa
     3121 acttcaccca tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct
     3181 tcaaaaaaat cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt
     3241 ccaaactcac ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt
     3301 actttttgaa tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa
     3361 cgataatcac tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca
     3421 aaacggcgtg tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt
     3481 tcttcaaaag ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct
     3541 ttcgtaccga actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata
     3601 gagccttctt ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct
     3661 tctagataag cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc
     3721 aaaggcgtgc cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt
     3781 tttccagcat cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct
     3841 acttcgattt cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat
     3901 gcttttggat tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt
     3961 tcacagttta atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact
     4021 ggcagcacgc ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca
     4081 aaatgagctg gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct
     4141 agtccaatga ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt
     4201 tatggaattc tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa
     4261 aataattacc gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag
     4321 acatacctgg aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg
     4381 tgattggatc gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga
     4441 tgatcaagtc ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt
     4501 gtcccgcttt tttgaagtaa gcgtcataat aaccagcact taaggagaat gtacctaaca
     4561 tgatacggcg ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat
     4621 ctaatgattt cacattttct gagcgataac cgtagcggat accatcgaat cgttgcaagt
     4681 tggaacttgc ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag
     4741 gcaagctgac ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata
     4801 cagcttcttt tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct
     4861 tcattccttt gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag
     4921 aagtaccgtc tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca
     4981 ccgtacgggt cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac
     5041 gtgagacacg gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc
     5101 ggatacttcc cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg
     5161 ctgcagaacc accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt
     5221 tcttgaaata agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac
     5281 caacagggat catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt
     5341 caaaattgta aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat
     5401 ccttgatacc aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta
     5461 aagcttttgc ctgttccaat gctttttctt cgttcaatgt aatgaatgct tcgacctcag
     5521 gctctgtttc tttgattcgt tcaaatgttg cttttgtcaa atcttgtaca gtgatttctt
     5581 tcgatacaag catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc
     5641 gccagcctcc ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg
     5701 cacatttttc aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac
     5761 gtttactgtt tcaaccacat tcgacgtaaa tggaacacct tctgtatcta cttcttccag
     5821 catttcaacc atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc
     5881 tgcgaacgca agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat
     5941 actacccctt ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc
     6001 gtgtctgaaa ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga
     6061 gcgactttta gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc
     6121 ttgtgctttg gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt
     6181 cgatacgcta ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg
     6241 agtaaatcgt gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac
     6301 ttcttgcaat tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa
     6361 ttcattcatc aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat
     6421 gatatcaccg atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc
     6481 tagatcaccg aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc
     6541 taacccgaaa atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat
     6601 attatttgct gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt
     6661 gtaaagatca gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg
     6721 ccccaaccca tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat
     6781 ttgagcaggg catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag
     6841 ttcacttcca caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg
     6901 tttatctaac acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac
     6961 tgtatctttc aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg
     7021 gcttactgtc gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc
     7081 tgttcgaccg actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa
     7141 cttgtaagct gctgcccatc tcggtgcttt gacagtaaaa ccaagttgat cttgtagcga
     7201 gaattcattc actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc
     7261 atgatattct tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg
     7321 attcgtgtgg aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg
     7381 cataggaccg aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat
     7441 tttagaatct aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc
     7501 ttttccctct gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca
     7561 ttcgcctctt acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt
     7621 ttttaggttt tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac
     7681 gaaatttcca tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca
     7741 atagtcaact acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc
     7801 tttgctgaaa acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc
     7861 ttctaatact tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata
     7921 ttgttcctca attgctacta attctgcgta ttctttatca taaacatagt cttccactgt
     7981 tggtttatct tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc
     8041 tgctcgttta gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact
     8101 aagctttttc aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag
     8161 ccacatctag ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc
     8221 attttttgtg tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac
     8281 ttttagaggg aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga
     8341 atacaggtgt ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga
     8401 tttcttctac aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga
     8461 aagcatttgt taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc
     8521 gttcttcttc cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt
     8581 ctagaccaat caggaataca actgggaatt ctaatccttt ggctgcatgc aaagtcatca
     8641 atgtcacttg ggaagcatct tcttctaaat tgtcaatatc ggaaacaagt gccagatcat
     8701 ttaagaagac cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga
     8761 attgtttgtc gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt
     8821 gtgcttctaa tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct
     8881 ctttcgtcaa ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct
     8941 ctccaaatgc acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt
     9001 gcgctgcttc aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag
     9061 atcctggtcc gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt
     9121 gaggatttgc cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga
     9181 acttgtgccc tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta
     9241 cacgagattg tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc
     9301 ttcgaatctc ttcttgcatc cgatctacga taaaacgggt ttcgtctcgt tcattatctg
     9361 cacggtagta agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc
     9421 gattgctatt gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat
     9481 agttttgttc tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt
     9541 tctgcatatc tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca
     9601 ggttcctaaa tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt
     9661 ggtactcatc tacatgaata taatggaatt tgttttgata atatgtgagt gaatcaggat
     9721 gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat
     9781 attgaagctc tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc
     9841 cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc
     9901 ctaatatact tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt
     9961 tcatcaaggt cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa
    10021 aatcaacatc tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat
    10081 cttcgcctcc agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat
    10141 tggtaaaagt aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg
    10201 caatgcggtg tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg
    10261 gtccgtcagt gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag
    10321 ctttggatga cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct
    10381 tggctttcat taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg
    10441 tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag
    10501 gaaaaatcaa gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg
    10561 gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt
    10621 tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta
    10681 tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca
    10741 agggaagcgg gattccacaa gtagagggac agcttcgcgg ggaattatca atgaactggt
    10801 tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc
    10861 taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat
    10921 tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac
    10981 ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc
    11041 actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg
    11101 tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc
    11161 cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta
    11221 tttatcagaa ggtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt
    11281 attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta
    11341 ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta
    11401 tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtggtggtc
    11461 ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg
    11521 gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg
    11581 ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga
    11641 cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt
    11701 atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac
    11761 ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa
    11821 cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc
    11881 tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga
    11941 atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg
    12001 aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa
    12061 actattttac aacaactatt catctacact acttgattct atatgattct ataaaaaaag
    12121 aagagactgg gaaaaaccag cctcttcttt tttatttcta tgcttctgct gctgcaggag
    12181 cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca
    12241 aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccatga ggagccagta
    12301 atttgatccc gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg
    12361 gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata
    12421 gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa
    12481 acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag
    12541 ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat
    12601 aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac
    12661 caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga
    12721 ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca
    12781 ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt
    12841 tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc
    12901 ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt
    12961 caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt
    13021 gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat
    13081 catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac
    13141 caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc
    13201 cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata
    13261 ctggtgcagt accactaagg gctagattga tcagctcttc tggtttacgg ataccatcac
    13321 tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg
    13381 ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc
    13441 cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca
    13501 gagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa
    13561 cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt
    13621 cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat
    13681 ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta
    13741 atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag
    13801 ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg
    13861 ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg
    13921 tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc
    13981 ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt
    14041 gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc
    14101 ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg
    14161 agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc
    14221 ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccggcaatca ttgaatcccc
    14281 tgcacctact gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc
    14341 cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc
    14401 tagcaatttt ttaccatacg gaatgatttc ttctacagat tggaaggttg tattgtaaag
    14461 ctcagctaat tcgtgattgt ttggtttgat caacaatggt ttcttaacaa gtgcattttt
    14521 taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat
    14581 cagttcttga taaaatcctt ttcgcaggct agccggcgcg cttcctgaca atacgacgat
    14641 atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat
    14701 agatgggccc aaaccgttaa tttcagtttc agtatctgat tttagtttga tattgattct
    14761 tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga
    14821 aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg
    14881 tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat
    14941 tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt
    15001 aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca
    15061 ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca
    15121 gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa
    15181 tttttcatgg ctaaacgttt aacagctgcc tcttctgggt ccggagtcgt gaatccttgt
    15241 tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt
    15301 tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata
    15361 atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact
    15421 gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa
    15481 taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg
    15541 tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc
    15601 ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt
    15661 cttatcgttg attcagaagc attaaaaagg tttacaagat cttgtgattt cacgattttc
    15721 tgctgttcaa gcaagtctag gattttttgt ctacgttcct ctgtaagcat tttcacgatc
    15781 acctcaatac aaataatagc atattttttt tctactcgca atataaatca gtcaaaaacg
    15841 ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcaaaaa gactggtttt
    15901 ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt
    15961 tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc
    16021 cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga
    16081 gacagacgga taataatgat gccggaacca ttattcgata atagagttgt ttatccattt
    16141 tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat
    16201 tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc
    16261 tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct
    16321 gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca
    16381 tctgttacct ctacttctag atattcccta atataataac aataagcttc gattccatta
    16441 tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgcgagcg attctcgtca
    16501 acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct
    16561 ccttgttgaa tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt
    16621 tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca
    16681 acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc
    16741 tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct
    16801 cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg
    16861 atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc
    16921 caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa
    16981 tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt
    17041 ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa
    17101 gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc
    17161 aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta
    17221 tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct
    17281 cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt
    17341 tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg
    17401 cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat
    17461 ttatcttcgc caatcatttt ctcacctctt tgaaatcttt ttataattta attataacca
    17521 acgataaaga aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag
    17581 actaagtaaa aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata
    17641 atttagataa acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag
    17701 aagagatcga tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac
    17761 aaaagtaagt taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact
    17821 ggtaagtctt ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc
    17881 ttcttttccc tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc
    17941 caacgctttt ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc
    18001 cttagtggca acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat
    18061 atcttcttga gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc
    18121 tctagtcgtt aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac
    18181 ttttgcatac ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa
    18241 attcaatgtc tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat
    18301 caatgtgttg ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg
    18361 caattgtgta tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg
    18421 gactttcatg gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag
    18481 ccaaaatgac ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat
    18541 agattactgt tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga
    18601 tgttttcatt tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac
    18661 gaagtcctcg agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt
    18721 caggttcgaa ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta
    18781 gcgcattttt cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag
    18841 ctgccataac aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt
    18901 caggaatgat atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa
    18961 atccaatcgt cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac
    19021 cacctacgat gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt
    19081 tacgtcctcc ctgaggtggc acgcttgcaa cagttccttc tagaattttc agcaaggctt
    19141 gttggacacc ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa
    19201 ttttatcgat ctcatcaata tagatgatcc ctttttctgc acgttccaca ttgtaatcag
    19261 cagattgaag caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg
    19321 tcaaacttgt cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg
    19381 ctaaaaatgt tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt
    19441 caacatcatc tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg
    19501 caacagctaa cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa
    19561 gaatttctag tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt
    19621 catcaatgat ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag
    19681 ctacgatctt tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat
    19741 tgctacttgg attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa
    19801 ctatcaaaga ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt
    19861 ttaacaaagg atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga
    19921 actgtgacaa aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct
    19981 tcttcagaaa tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga
    20041 tcaattcgat tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc
    20101 gtttgatttg ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga
    20161 tttcttcttc tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg
    20221 ttcttgtgcg catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac
    20281 cagttaattg atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt
    20341 cgtccattga acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg
    20401 cgttttcaac agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt
    20461 ttgtttctac taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt
    20521 catcaacatc ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat
    20581 ggattgtcac tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg
    20641 ggaaagttac atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa
    20701 aacctggaat gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat
    20761 caaacgcttc gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag
    20821 ctgcagcatc ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg
    20881 catccacgtc agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt
    20941 gtcctaattt tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt
    21001 tttccatgct ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga
    21061 cagcagcttc ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt
    21121 caccgtacat acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt
    21181 tcaagctttt tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg
    21241 caatcgtaaa ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt
    21301 aattccctcc gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta
    21361 aatagtacac taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc
    21421 ataacatttt tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa
    21481 ttttttctct tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt
    21541 ttcctgtaca cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat
    21601 catagacagg ataaacaatt gcagattgca acgtgaattc ttcatttaac acggaactaa
    21661 gcagcgcatc ttcattaccg taacgatctg aaaatacaca taattgctgg taacttgcct
    21721 gctgctgcgg ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca
    21781 aatagaaaac aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg
    21841 ctaaaatatg gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat
    21901 aaggaagctg ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa
    21961 gttcctccga tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt
    22021 cttctgcaaa ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac
    22081 ttgctatcaa ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa
    22141 attcagcaaa ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg
    22201 cttctttttt ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc
    22261 tttctttcaa ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg
    22321 ctagtgcttg ttgtgctact cgcattaaat tctcatattt ttctggaaaa tcaatcggct
    22381 tcacgcagtg tcctcctctt gctcatgttc agctcatgtt caatagtagc aaaacaaaaa
    22441 aagcatgaca attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt
    22501 ttagaaggtg ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt
    22561 tgaatgcttt gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcctagat
    22621 gttgcttttt ctgattaaag attgctggaa gataccctaa catcagcaaa ggaaatttta
    22681 gaatgaaccg gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat
    22741 caaagctgat gactcgaata ccaaagatca ttttcccaat cgtttgacca tgattgaatt
    22801 tcgtcaataa aataaaatac gctagataaa taagtaaggc tgtgaatccg tagatactca
    22861 gataagaatc tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg
    22921 taatagctcc gatacacaaa agatcgacca agaaagcaaa cattcggata aaaaaacctg
    22981 catagaaata cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact
    23041 ttttccattt acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag
    23101 agctcagtac cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat
    23161 ctttttcttg ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata
    23221 atacattgct tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact
    23281 agtctcagaa ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc
    23341 aaacccagat ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt
    23401 tcgcatagct tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt
    23461 tgcttggaca ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt
    23521 attccgtccc tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg
    23581 aagcaccgct tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc
    23641 tccgctttta actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa
    23701 tcctgacatg atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc
    23761 tgcttgagca gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat
    23821 tgggatatca tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta
    23881 gacaccgcca ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc
    23941 aatcgccttc agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc
    24001 tgaagaccct cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact
    24061 gtctccctct tccaacacat ttgaagctag ttcttcatcc ccatataacc aactgttcaa
    24121 tccctcaatt tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac
    24181 aaataatcct gcagcaatca gaactgctgc ccaacgtctc ttattcattc tttctccttt
    24241 ctaatacaag ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc
    24301 tgctttttct tcccgcgcat acccggaaaa cgactctttt gtcaattcat cttctaccgt
    24361 aaactcacga gcttcagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca
    24421 gccagtatct aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga
    24481 caattgtttt tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc
    24541 tcctctatcc gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc
    24601 cattattttt tgacataaaa agagcatttg acaaagcatt tgatatatac caatttattt
    24661 tttattcttt ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag
    24721 ataaaaaaca aaagaggagg atagttatac cttttaggat agcgccaggt ctgtgtaaac
    24781 agatgacgag gagagggttt atcgaaagat tcggcggatg gccctaggga ctgcactcta
    24841 cagaaaatcc acaaaaaata tctgcaaaga taaaacaaaa ggattttcct gcagctggaa
    24901 gcattcatac gaattcatta tagaaagtag gatgtttttt atgtgcggaa ttgttggaat
    24961 gattggatta aaaaatgtaa caccaggatt gattgacggt ctagaaaaat tagaataccg
    25021 tggttatgat tcagctggta tctttgtctc agatggcaca actgattatc tggtcaaagc
    25081 acaaggcaga attcaaaatt tgaaaaacaa aataaatatt gatactacag gaaatatagg
    25141 tattggtcat acacgctggg caactcacgg gcaaccttct gaagaaaacg cccatccaca
    25201 tacttcccaa agtggacgct tcgtgctcgt tcacaatggc gtaatcgaaa actttgaaga
    25261 acttaaaatt gcttatttag caaatgacca ctttattggt gagaccgata ctgagattat
    25321 cgctcatcta attgaaactt tcgcgaagga tactacaacg caagaggcgt ttttaaaagc
    25381 tttgcgggtc atcaaaggtt cttacgcttt cgctttgatt gatcggacag cacctgatgt
    25441 tatttacgtc gctaaaaata aaagtccact attaatcgga ctcggcgatg gattcaatgt
    25501 gattgccagc gatgcaatgg ctatgcttgc tcatacgaag gaattcgttg agattgaaga
    25561 tgaagagatg gttaccgtca catcagaaaa agttattatt caaaattttg caggtgacat
    25621 cgtagaacgt tcttcattcg aagcacaagt agattcttca gatatcgaga aaggtaccta
    25681 tcctttttat atgctgaaag aaatcgacga acagcctatt atcatgcgtc gtatcgctca
    25741 aaaatacgta acagaagaca atcgtgtctc tcttgatcaa aagctggtcg atacattgtc
    25801 tgatagcgat cgaatctata tcgttgcttg cggaactagt taccatgcgg ggttagcagg
    25861 taaacagact ctggaaaaat tgacacagat ccctgttgag gttcatttag ccagtgaatt
    25921 tggctataac acacctcttc tttcaaaaaa accattcttt attttcttga gtcaaagtgg
    25981 cgaaacagca gatagccgcc aagttctggt aaaaatcaac cgtctcggct acccttcact
    26041 tacgattaca aatgttgctg gttctactct ttcgcgggaa gcatccttta cactgctatt
    26101 gcatgctgga cccgaaatcg cagtagcatc aacgaaagct tatactgcac aaatagctgt
    26161 actaacttta ttagccaaag ctatcggtga taaaaaagaa tttgaaactt ctcttgtatt
    26221 cgatatcact catgaattga gtttagttgc taatgcgatg gaatcggtca tcgcccaaaa
    26281 agattattta gaagagttgg cagaagtata tctcagcgat acaagaaatg ccttttacat
    26341 tggacgtggc gctgactaca acgtttctct tgaagctgct ttgaaattga aagaaatcag
    26401 ttatatccag gcagaaggtt ttgccgctgg cgaattgaaa catggaacga ttgctttgat
    26461 cgaagaagga acaccagtga ttggaatcat cagtgaagaa gtgactggtg cgcatactcg
    26521 aggaaacctg aaagaagtcg aaagccgtgg cgctaaaaca ctagttattg tctcagaggg
    26581 attagaaaag gaaagtgacc agctggtgct accaactgtc cacccttatc taactactct
    26641 aaccacagtc gtaccgactc aattacttgc atattacgca acgctattac ggggattcga
    26701 cgttgacaaa ccacgtaatt tagcgaaatc agtgacagta gaataataaa agaacaagga
    26761 ccagtgtcag acatctgaca ctggtccttg ttcttttatt ttttatgaaa atatctctta
    26821 atccagtccg atttcttctt tgactactgc cgcgatttta tcgacatagt aattaacttt
    26881 ctcatcagta ggcgcttctg ccatcactcg aagtaatggt tctgtaccgg aaggacgaac
    26941 taggattctt ccttctgatc ccatttcagc ttctgcctct tcaatcactt ttttgattgc
    27001 cggaacatcc atcgcaccgt ttttatcact tactcggata ttcactaatt tttgcggata
    27061 gattgttacc tctgctgcaa gctctgataa tttcttacct gtctgtttca tgatattcag
    27121 taactgaatt cctgaaagca tcccatctcc agttgtgtta aaatctaaga aaatcatgtg
    27181 cccagactgc tcgccgccaa agttataatc gtttttgcgc atttcttcta caacgtaacg
    27241 atcaccaact tgtgtaacga catctttcaa accgattcct tccaccgctt tgtggaatcc
    27301 taagttactc atgacagtcg taacgatcgt atcttttttc aatcgatttt tttctgccaa
    27361 gtatttagca cagataaaca tgattttatc accgtcgaca atgtttccaa gttcatccac
    27421 cgcaataata cgatcgccat cgccatcgaa agctagtcca gcatctgctc ctttttcaac
    27481 gaccatctca gctaaacgtt ctggatgggt agagcctacg ccatcattga tatttaaccc
    27541 gtctggattt gtgcccatgg tataaaaatc tgtttcaaga tcggcaaaca aacgattaac
    27601 acttgttgaa gttgctccgt ttgctgcatc gacacaaatg gtcaaaccgg aaaggtcacc
    27661 gttgatcgtt tgtacaagga attgagaata tttcaataag ccttctggaa attcttctac
    27721 agttcccaat ccttcagctg atggacgagg caattcatcc acttcagcat ctaataatgc
    27781 ttcaatttct agctcttggt catctactag tttaaaacca tctgcaccga aaaatttgat
    27841 tccattatct tgtgctggat tatgagaagc agaaatcatg acccctgcgc ttgctttttg
    27901 caaacgtgtc agataagcaa cacctggtgt agagatcaca cctagctgga acacttcgat
    27961 acccaccgat aaaagtcctg aaatcagcgc ttcttctaac atttgacctg agattcttgt
    28021 atcccgccct actaacacgc gtgggcgttt tccttcttct tcatgctggc ttaggacata
    28081 acctccgcac cgtcccagct taaatgcaag ttctggcgta agttccttgt tggcttcacc
    28141 gcgtacgccg tctgttccaa aatatttacc catagttgta aaattactcc tttaacttaa
    28201 ttttctgagg aatttgttct gctcatttgt gttgattcgc ttgtttctga tgtttcttct
    28261 gtacttgtgg tcgagctgct tgtcccagat tccgaactgg aagaaatggt tgttgtcgat
    28321 ggttccgttc ctgaatcagc agatgaaata cttctactcg tacttgtatt ttcttgtgtc
    28381 cgactgctcg tcgaagcaga gctactgctt gacgtttgca acacgggtgt aatctccaca
    28441 ctaactgatt cgggcttgat acttaacccc tcttctactg gtatttcaat tttacgagtt
    28501 gttcgatgat tgatacctga gacattgatc ggtacagcaa tactgtcacc gatcgatgca
    28561 agcaaatcgc tagagccgct tacttctgct tccagttttg aaaaactgaa atcatagctt
    28621 tctaccccgg cagctggtgt tccttgctgt atcccataaa gtcgtaccga cttgcttggc
    28681 ttgatcgaat cagctctcac tgttacttgc actggatcag agacgatact taatacttcc
    28741 cctgctgcat tcagcgcctg tattgggacc tcactattaa tcccatcatc tgtgaccatc
    28801 gaagcgtcca catcagctac tactcgtttg atttctgcca atgttttgtc acctgtagtg
    28861 atagagactt ttttaggact aacggaagta tcatcaagtt catatccaga tggtgtgaac
    28921 gttgaaggaa tcaacgtttc cacatcgaaa tcttttatta cctttttctc aaccgttact
    28981 gtaatagtcg atggttcgat ctttgctgtg acagcactgc tcaaattttg aatttttagc
    29041 ggaacctcat gtgttccttc gcccagctta gtgatatctg ctgtgacgcg gaacatccgt
    29101 gtatcttcgt ttgcctctgc attcaactga actcggtttg cactggaaag tttgacagtc
    29161 actgcatttt catatccatg gatatagtag cgctttgaat cataagaaat cttgattggc
    29221 accccagtaa ctgtttgatt atacgcttca tttccagaaa gcgtatttgg aacggactgg
    29281 ccatttgcat tgaagtacag cgcaatactg aacagcaatg caataaaact gtaaagaatg
    29341 cttgtccgtt tctcttttga gatcatttgc gatcccctcc cttagtcata ccgtctagga
    29401 aagattgaat cacgtttttc ttgttttttg ttttctcttc agcaatcagt tctctacgca
    29461 aaattgccaa atattcttgc tgggataagc cagccatcag ttcattatct aatgtaatgc
    29521 tgacatcccc agtttcttca gagacaacga tggtaatcgc gtcgctgact tcgcttatac
    29581 cgacagctgc ccggtgtcga gtcccgaatt cttttggaat caacatactt tctgatagag
    29641 gaagataggc gcttgctact gcgatctttc cttcttttac aataactgca ccgtcatgca
    29701 gcggtgtatt aggaataaag atattgatca gcagctctcc agtgatatct gcatctaatg
    29761 caattcctgt ctcaatatac tcatctaatc cagtatgccg ttcgatagta attagcgcac
    29821 cgatttttcg cttggacata tattggatgg ctttgtcaaa agacaagatc atcttctcat
    29881 cttcctgctg ttcgcttttt gattgtttga aaagcgaact gcgtccaaga tgttccagtc
    29941 ctctccggat ttccggctga aaaatcacga tagcagctat caccccgtac gtaatgactt
    30001 ggttcatcag ccaagacaag gtgtgcagac caatgatttc cgctacgatt cggataacaa
    30061 taaaaatagc tacacctttc agaagctgaa tcgcttttgt gcctcgcagc agttggatca
    30121 gtttgtatac aagataccag acaaccagaa tatcaagtat gttcaccaaa aaattcagag
    30181 agaacaagtc accggaaaac atttgctgcc aatagtccaa attaaaaagt tccccgattt
    30241 gaaatgacat aaaaagcctc tcttctcttt tgagatttcc tctctagtat atcatatgtt
    30301 ttaattttag aggctgatct gctcgtattt ttcttgagaa aaacgcataa ttttcaggaa
    30361 aattgcaagt atgcgtttta tttttctctg caataaatat tttt
//
LOCUS       DAXWLB010000014        29108 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXWLB010000014 DAXWLB010000000
VERSION     DAXWLB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.014"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1031)
                     /locus_tag="ACGE4H_000500"
     CDS             complement(<1..1031)
                     /locus_tag="ACGE4H_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8363941.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACGE4H_000501"
     CDS             complement(1113..1802)
                     /locus_tag="ACGE4H_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8363942.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGE4H_000502"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACGE4H_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFF8363943.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGE4H_000503"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACGE4H_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFF8363944.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGE4H_000504"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACGE4H_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFF8363945.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGE4H_000505"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACGE4H_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFF8363946.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGE4H_000506"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACGE4H_000506"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFF8363947.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGE4H_000507"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACGE4H_000507"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFF8363948.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACGE4H_000508"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACGE4H_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACGE4H_000509"
     CDS             complement(7636..8523)
                     /locus_tag="ACGE4H_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8363949.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGE4H_000510"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACGE4H_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFF8363950.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGE4H_000511"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACGE4H_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFF8363951.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGE4H_000512"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACGE4H_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8363952.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGE4H_000513"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACGE4H_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFF8363953.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACGE4H_000514"
     CDS             complement(10454..10957)
                     /locus_tag="ACGE4H_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8363954.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACGE4H_000515"
     CDS             11554..12519
                     /locus_tag="ACGE4H_000515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8363955.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACGE4H_000516"
     CDS             complement(12618..13790)
                     /locus_tag="ACGE4H_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363956.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACGE4H_000517"
     CDS             complement(13777..14922)
                     /locus_tag="ACGE4H_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8363957.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACGE4H_000518"
     CDS             complement(14936..15868)
                     /locus_tag="ACGE4H_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8363958.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACGE4H_000519"
     CDS             15985..17064
                     /locus_tag="ACGE4H_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8363959.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="ACGE4H_000520"
     CDS             17061..17690
                     /locus_tag="ACGE4H_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8363960.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17800..18027)
                     /locus_tag="ACGE4H_000521"
     CDS             complement(17800..18027)
                     /locus_tag="ACGE4H_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8363961.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18130..20718)
                     /locus_tag="ACGE4H_000522"
     CDS             complement(18130..20718)
                     /locus_tag="ACGE4H_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFF8363962.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20738..21397)
                     /locus_tag="ACGE4H_000523"
     CDS             complement(20738..21397)
                     /locus_tag="ACGE4H_000523"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8363963.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACGE4H_000524"
     CDS             complement(21446..21949)
                     /gene="trmL"
                     /locus_tag="ACGE4H_000524"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFF8363964.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22170..23036)
                     /locus_tag="ACGE4H_000525"
     CDS             complement(22170..23036)
                     /locus_tag="ACGE4H_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8363965.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23145..23786
                     /locus_tag="ACGE4H_000526"
     CDS             23145..23786
                     /locus_tag="ACGE4H_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFF8363966.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACGE4H_000527"
     CDS             complement(23848..25209)
                     /gene="mgtE"
                     /locus_tag="ACGE4H_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFF8363967.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25227..26126)
                     /locus_tag="ACGE4H_000528"
     CDS             complement(25227..26126)
                     /locus_tag="ACGE4H_000528"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8363968.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKSYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26128..26925)
                     /locus_tag="ACGE4H_000529"
     CDS             complement(26128..26925)
                     /locus_tag="ACGE4H_000529"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFF8363969.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26903..27607)
                     /locus_tag="ACGE4H_000530"
     CDS             complement(26903..27607)
                     /locus_tag="ACGE4H_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFF8363970.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27738..28313
                     /locus_tag="ACGE4H_000531"
     CDS             27738..28313
                     /locus_tag="ACGE4H_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFF8363971.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28431..29108
                     /locus_tag="ACGE4H_000532"
     CDS             28431..29108
                     /locus_tag="ACGE4H_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFF8363972.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6168 c         5232 g         9143 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    17761 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    17821 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17881 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17941 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    18001 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    18061 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    18121 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    18181 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    18241 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    18301 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    18361 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    18421 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    18481 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    18541 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    18601 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    18661 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    18721 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    18781 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18841 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18901 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18961 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    19021 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    19081 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    19141 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    19201 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    19261 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    19321 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    19381 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    19441 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    19501 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    19561 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    19621 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    19681 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    19741 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    19801 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19861 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19921 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19981 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    20041 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    20101 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    20161 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    20221 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    20281 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    20341 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    20401 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    20461 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    20521 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    20581 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    20641 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    20701 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    20761 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    20821 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20881 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20941 ctacgaataa aagtgggcca tctttatgtc gggcaaccgc atctgctaca acagtttcga
    21001 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    21061 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    21121 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    21181 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    21241 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    21301 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    21361 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    21421 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    21481 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    21541 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    21601 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    21661 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    21721 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    21781 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21841 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21901 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21961 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    22021 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    22081 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    22141 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    22201 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    22261 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    22321 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    22381 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    22441 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    22501 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    22561 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    22621 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    22681 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    22741 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    22801 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22861 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22921 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22981 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    23041 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    23101 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    23161 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    23221 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    23281 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    23341 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    23401 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    23461 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    23521 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    23581 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    23641 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    23701 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    23761 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    23821 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23881 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23941 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    24001 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    24061 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    24121 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    24181 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    24241 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    24301 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    24361 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    24421 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    24481 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    24541 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    24601 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    24661 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    24721 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    24781 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24841 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24901 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24961 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    25021 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    25081 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    25141 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    25201 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    25261 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    25321 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    25381 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    25441 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    25501 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    25561 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    25621 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    25681 gcaaatccat gctttgcaaa aagcatcaag ccagatgtat cccgatctag tcgcgtcacc
    25741 acatggatta cttggttttc gtaaccttgt tttttataat aagactttac acgattagcc
    25801 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25861 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25921 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25981 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    26041 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    26101 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    26161 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    26221 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    26281 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    26341 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    26401 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    26461 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    26521 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    26581 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    26641 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    26701 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    26761 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    26821 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26881 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26941 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    27001 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    27061 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    27121 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    27181 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    27241 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    27301 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    27361 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    27421 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    27481 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    27541 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    27601 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    27661 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    27721 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    27781 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27841 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27901 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27961 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    28021 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    28081 cattaatcca aaccggaaag cttacaacaa aacgagctga attccctatt gaagaaggac
    28141 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    28201 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    28261 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    28321 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    28381 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    28441 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    28501 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    28561 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    28621 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    28681 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    28741 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    28801 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28861 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28921 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28981 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    29041 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    29101 ctaattaa
//
LOCUS       DAXWLB010000015        28293 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXWLB010000015 DAXWLB010000000
VERSION     DAXWLB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.015"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            214..2049
                     /gene="tdc"
                     /locus_tag="ACGE4H_000533"
     CDS             214..2049
                     /gene="tdc"
                     /locus_tag="ACGE4H_000533"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8363973.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2354..3832
                     /locus_tag="ACGE4H_000534"
     CDS             2354..3832
                     /locus_tag="ACGE4H_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8363974.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3798..5129
                     /locus_tag="ACGE4H_000535"
     CDS             3798..5129
                     /locus_tag="ACGE4H_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8363975.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5243..7795
                     /locus_tag="ACGE4H_000536"
     CDS             5243..7795
                     /locus_tag="ACGE4H_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFF8363976.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7802..9241)
                     /locus_tag="ACGE4H_000537"
     CDS             complement(7802..9241)
                     /locus_tag="ACGE4H_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8363977.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9216..9398
                     /locus_tag="ACGE4H_000538"
     CDS             9216..9398
                     /locus_tag="ACGE4H_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8363978.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9587..9949
                     /locus_tag="ACGE4H_000539"
     CDS             9587..9949
                     /locus_tag="ACGE4H_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFF8363979.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10136..10993)
                     /locus_tag="ACGE4H_000540"
     CDS             complement(10136..10993)
                     /locus_tag="ACGE4H_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8363980.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11113..12024
                     /locus_tag="ACGE4H_000541"
     CDS             11113..12024
                     /locus_tag="ACGE4H_000541"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFF8363981.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12031..13677
                     /gene="mdcA"
                     /locus_tag="ACGE4H_000542"
     CDS             12031..13677
                     /gene="mdcA"
                     /locus_tag="ACGE4H_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFF8363982.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13779..14078
                     /locus_tag="ACGE4H_000543"
     CDS             13779..14078
                     /locus_tag="ACGE4H_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFF8363983.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14068..15744
                     /gene="mdcD"
                     /locus_tag="ACGE4H_000544"
     CDS             14068..15744
                     /gene="mdcD"
                     /locus_tag="ACGE4H_000544"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFF8363984.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15748..16341
                     /locus_tag="ACGE4H_000545"
     CDS             15748..16341
                     /locus_tag="ACGE4H_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFF8363985.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16365..17441
                     /locus_tag="ACGE4H_000546"
     CDS             16365..17441
                     /locus_tag="ACGE4H_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFF8363986.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17466..18335
                     /gene="mdcB"
                     /locus_tag="ACGE4H_000547"
     CDS             17466..18335
                     /gene="mdcB"
                     /locus_tag="ACGE4H_000547"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFF8363987.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18446..19210
                     /locus_tag="ACGE4H_000548"
     CDS             18446..19210
                     /locus_tag="ACGE4H_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFF8363988.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19699..21792
                     /locus_tag="ACGE4H_000549"
     CDS             19699..21792
                     /locus_tag="ACGE4H_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8363989.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21817..22119
                     /locus_tag="ACGE4H_000550"
     CDS             21817..22119
                     /locus_tag="ACGE4H_000550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8363990.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            22146..23435
                     /locus_tag="ACGE4H_000551"
     CDS             22146..23435
                     /locus_tag="ACGE4H_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8363991.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            23454..23861
                     /locus_tag="ACGE4H_000552"
     CDS             23454..23861
                     /locus_tag="ACGE4H_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8363992.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            23884..24753
                     /locus_tag="ACGE4H_000553"
     CDS             23884..24753
                     /locus_tag="ACGE4H_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8363993.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            24755..25375
                     /locus_tag="ACGE4H_000554"
     CDS             24755..25375
                     /locus_tag="ACGE4H_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8363994.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            25825..26430
                     /locus_tag="ACGE4H_000555"
     CDS             25825..26430
                     /locus_tag="ACGE4H_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8363995.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            26565..27017
                     /locus_tag="ACGE4H_000556"
     CDS             26565..27017
                     /locus_tag="ACGE4H_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8363996.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            27054..27551
                     /locus_tag="ACGE4H_000557"
     CDS             27054..27551
                     /locus_tag="ACGE4H_000557"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8363997.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            27569..28279
                     /locus_tag="ACGE4H_000558"
     CDS             27569..28279
                     /locus_tag="ACGE4H_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFF8363998.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
BASE COUNT         9303 a         4838 c         6023 g         8129 t
ORIGIN      
        1 tattttagaa gactgattta ttgaaaaatt gttcgctttt ttgcgttgaa ttatcagaaa
       61 aatccgactt tatttatttt tgtttgtaaa aatctacgtg gatttgatgt tataaaaaaa
      121 aaattctcat ttttggcgct atagtgaaga ggtgttacag ataaacacat gtatctgcaa
      181 gacattgacc aacaaaatat ggagggaaga aaaatgaaag atatggatat caaggccgtc
      241 tttataggcg acaaagctga aaatggacca gtttataaaa tgttgttgaa caagatggta
      301 gatgaacatc ttggatggag agaaaattat attccttcag atatgcctgc aattagcgaa
      361 ggagataagc tcactccaga ttatctagcc acccgcgacc atatgataga ggtcttggat
      421 gaagtcagtc agcgattacg cgcaggatcg atcccatggc attcagcagg ccgttactgg
      481 ggacaaatga atgctgaaac attgatgcct gctttattag cttacaacta tgcgatgctg
      541 tggaacccaa ataatgttgc attggaatct tctatggcaa cttcgcaaat ggaagcagaa
      601 gtaggacagg attttgcttc attattcaat atgacagacg gatggggaca cattgcagca
      661 gatggttcta ttgctaatct tgaaggatta tggtacgccc gttgcatcaa atcgattcct
      721 ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa tgtctgagtg ggaacttttg
      781 aatttatctg ttgaagaaat tttggaaatg accgaaagct ttacggacga agaaatggat
      841 gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc aaagattagg gaaatggctg
      901 gtccctcaaa caaaacacta ttcttggatg aaggcgttag atatttgcgg agtcggctta
      961 gatcagatgg ttgctatccc agttcaagag gattatcgaa tggatattaa tgctttggaa
     1021 aaaacaattc gtgaattagc tggacaaaaa atcccaattc taggtgtggt tgctgtcgta
     1081 ggaacgacag aagaaggtca agtagacagt gtagataaaa tcgtccaatt aagagaaagg
     1141 ttaaaagatg aagggatcta tttctattta catgttgatg ccgcatatgg cggctatgcg
     1201 cgttcactgt ttctaaacga agcaggagaa tttgtgccgt atgcttcctt agctgaattc
     1261 tttgaagaac atcatgtttt ccaccactgc gtaacgattg acaaagaggt atatgaaggg
     1321 ttcagagcta tttcagaagc ggattctgtc acgatagatc ctcataagat gggctatgtc
     1381 ccttatgctg ctggaggaat cgtaatcaaa cataaaaata tgagaaatat catttcttat
     1441 ttcgcgccat atgtgtttga aaaatcagta aaggctccag atatgttagg agcttatatt
     1501 ttggaaggat caaaagctgg agcaaccgcc gccgcagtct ggacagcaca tcgtgtattg
     1561 ccattgaatg tgaccggata tggtcaattg attggggctt caatcgaggc agctcaaaga
     1621 ttcagagaat ttctcgatca tctgactttt actgttaaag gaaaaacaat cgaagtctat
     1681 ccattgaatc atcctgattt taatatggtc aattgggtat tcaaagaaca aggctgtaca
     1741 gatttgaacg ctatcaatga attaaatgaa aaaatgttcg atcggtcttc ttatatggat
     1801 ggcgatgttt atggtgaacg gtttattact tctcatacta catttacaca agaagattac
     1861 ggtgattctc cgattcgttt tgttgaaaga atgggtctaa cgaaagaaga atggaaaaaa
     1921 gaacagaaaa tcactctgct acgtgcggct atcatgactc cttatttgaa tgatgatcga
     1981 attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg aaaagaaact aaacgaaatc
     2041 atccaataga actgattttt gtgtgaaaga ataacaagag acatatggag aacagacaat
     2101 tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt catatattcc tcgccaagat
     2161 attctttttt ataaaacgtt ctataagttg gatcgttcgt ttcatgtatc aaacccagtt
     2221 cactaagatc gttcaatgga tgtttcggtg atactgggtt ttgtactgaa tcgatgaaac
     2281 cttgtcattt ctgttcttga acaaaaataa aagaaaacaa aaacatatgt cgctattgaa
     2341 agaaggaata aagatgaaag atactggttc atcaaagcaa atcacgacca tgacctttat
     2401 cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt ccagatgtag cagcaacgag
     2461 ctggacgatg attttttaca tgctggttgc agtattattg tacgcttttc caatctcatt
     2521 gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt ggtccagaac tttgggtatc
     2581 gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg ctgttatggg tccaaatgtt
     2641 tcctgggatg gtgatggtcg cttcagcact cgctccgttg tttggtaata ttatcgataa
     2701 tgtgccgctt ggtttgaaca gcaaatttac attagttgtc attttagtcg tttattggat
     2761 catcactttt ttgaatttga aatttgatat ggcaaaaatc ggcggaaaag tcggtgtttg
     2821 gttaggattg tacgtgcctt tgacgatgat gctcttatta gggtttgctg cgtggatcaa
     2881 agttgggatc gtaccaaccg gaacattagg cagtttttca tgggacaaat taataccaga
     2941 tactacgact gcatcctcat ttgtttattt tgcgccgatc atgtttattt ttactggtat
     3001 cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca gttaaaacgt atattcgcgg
     3061 agtatttgcc gcgttaattt ttatattttt cgtcaatatt ttgaatgctt tagttgtagc
     3121 aaatgtcgta ccaaaaggac aaatggaatt gaataatatc gcgcaatcta tttcgattta
     3181 ttgccaaatt cttggtttgc cacatcttat tgtgaatctg tttagtttac ttgtcttcat
     3241 tggtgtagca gtccaattat ctgcttgggc ctcaggacca gcaaagacag tgacagaaag
     3301 tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg aagacgaatc aatttgacgt
     3361 atccaaatcc gttattttga ctcagtctgt cattatctca atctttgcac ttttttattt
     3421 gctgattcct ggcgtcaatc aagcattttt gatgctcgta aattcaacta ctgttatcta
     3481 ctgtatcgtc tatgtcatca tggggatcgc agttttgaga ttgcgttata cacatgcaaa
     3541 gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag agcaatcttg gagtttggac
     3601 tgtcgttatt gttttatttg cggcaatcgg attttctgtc ggactgacga tgaaagctgg
     3661 aacatggatc aatctaattg cagtcacagc catcagtgtc attttgtttg tagtaccact
     3721 ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa gtcgccatgc tgcttgaaaa
     3781 agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg gactctaact aatcgaaata
     3841 tccagttaat cgcattaggt ggcgcaattg gaacaggatt atttttaggt tcagcaaagg
     3901 caattgcatc agcagggcca gctgttattc tcgtttattt gatatgcggt ttggctattt
     3961 atggtttgat gcgggccatg ggtgatttat ttttagcaaa tagtacattt aactctatca
     4021 gtgatttcgt tgactattat ttaggcaaaa agtgggcatt ttttgtcaat tggacttatt
     4081 ggctttgctg gcttggagct ggacttgcgg aattaacagc aataggattg tatatccatt
     4141 tctggtttgt acatgtacca gctttgataa gcggaggtag cgcactaatg atcttaatgg
     4201 cagtcaacct tgttgcagtc aagtggtttg gtgaattgga atcttttttc tcaatcatca
     4261 agatcttggg gattttcctt tttatcttat tgggtatagg attgagtgtc tcagattttc
     4321 atacccagtc tatcggaatc aaagaaaatt ttggacagat cgatgaatta ctaccgtttg
     4381 gctttgctgg aatggtagtc tcatttccta tggttttatt cagttttgct ggaattgaaa
     4441 tgatcggctt aaccgttggc gagacaagtg atccgcaaaa gaatttgaaa aaagcaatca
     4501 actgtctgcc ttggcgtatt ctctttttct atataggaac gattttgtcg attttatttg
     4561 ttgttccatg gcattgttta gatttaaaag aaagtccttt agtgactatg ctgcaagtga
     4621 tccattgcca aggcggagca gcaattgtca atctaatgat tttggttgct tctttgtcct
     4681 cagcaaatag tgcagtctac agtactagca ggattttata tcaaacagca aaagaaaaaa
     4741 gtttgcccca ttggttttgt gcgctttcta agaaaggcgt acctttacac ggtattttaa
     4801 ttacaggaat gttatttttt gccggtcttt gtctacattt ttttgtagct gatagtcgat
     4861 tgttttttca gcttttggca ggcatgacaa cgagttgttt tctatttgtg tggtcttcga
     4921 tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg gaacactaca caaagatata
     4981 aagatcttgc tttgttggtg ttttttactt caattgcttt cagcttgtta ttcgaaagca
     5041 cgacacgctt tattcctttt ttattggtta tctggtttgt attcttaaat tttgtatata
     5101 aaagaataag caatagaaat atcagttagg cccagataca ttttgcaaaa cgaattattt
     5161 cagacccccc tgaaataatc gatacgcctg aaaaaacttc cctgcgtata ataacagtat
     5221 caattaaaca ggaggggata ggatgaactg gtacagattg acggtagaag atgtattgaa
     5281 aaactatcaa acatcaacag aaggcttgtc gcaagaagaa cgacagaaac gcctgactga
     5341 tcaaggatat aatgaaataa aagcaaggca gcagacaaaa cgatggaaaa aaatagcaaa
     5401 gcattttact gatctgctga tgatcgtgtt gatcattgcc tcttttttga aatttgcttc
     5461 tagtgaatat attgaaggaa gtattatttt attcgtagtg atcgtcaatg gactcgttag
     5521 ttactggcaa gaaagaaaag cggaagaatc attgaatggt ttgaagcagc tgatgggaca
     5581 agaagcgata gtactatcaa acgggatgca agaaaaaatc cctgcgaaag aactggtaaa
     5641 aggagatgtc attgtcttac gtgccggaga tgtcgttcca gcagatatcc gattaatcga
     5701 agcacatgat ttattagtgg aagaatcgat tttgacggga gaatcagaag catctgaaaa
     5761 aagtcatgcc gttttgacag aagaagaatc gatcggcgac cagaaaaata tgggtttttc
     5821 aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt gtagaaacag gaaacacgac
     5881 agaaatcggt aaaatcaatc aagcattgca atcggtcgaa caacaaacga cgcctctgat
     5941 taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc attctttgtt tgagcctatt
     6001 cttagttatt tttacgacat ttcgatatgg tatggattgg catattcttc tttcggctac
     6061 gattgctttg gtggtgtcaa tggtacccga aggattgcca gctgttttga ccatgattct
     6121 atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc atcaaaggac ttccttcggt
     6181 agaaacatta ggttctatga cagtgatctg ttcagataag acaggaacat tgacaaaaaa
     6241 tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc tgcgtgttgt ccattatgaa
     6301 aaactgtcag gaattaaaaa caaaagaaga acagaaaaca gaaaatttaa gtggcaatcc
     6361 gacagaagtt gcgctgattc gacacacaga aacagccaat ctacctttga aaaaaataga
     6421 agcaaagatt ccgttcagtt ctgaatacaa atatatggca acgatgcatg cggaggaaaa
     6481 aggagcggtg atttacgtca aaggtgcacc agaagtatta tttgctaaat caactctttc
     6541 ttctgcagaa caagaagcat ggagtcagac agctgcagaa tttgcgagaa aaggtcagcg
     6601 tgttttagga tttgcttata aaaaagtaga ttcgaaacag gaactcacgc atgagacttt
     6661 aacccaatta acttttgcag ggatcgccgg attgatcgat ccgccaaaag aaagtgcagt
     6721 caaagctgta aaggaatgcc aacaagcagg gatctcagtg aaaatgatta caggggatca
     6781 caaagacaca gcaaaagcga ttgcggaaca aattggctta aaacatactg ccaaagtttt
     6841 agaagggatc gatttggatc ttatgtctga tgaagagttg attcagcaag tgccaatagt
     6901 cgatgtcttt gctcgaacga caccagaaca taaactgcga attgttaaag ctttgcagaa
     6961 aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat gatgcacctg ctttgaaaag
     7021 atccgatgta ggaattgcta tgggaatcaa aggtagtgaa gtaagcaaac aggcagctga
     7081 tatggtttta ggagatgata atttccatac gattgcaaaa gccgtaaaag agggccggcg
     7141 gattttggat aatttgcaaa aaaccatcaa tttcttcttg ccaactgcat tagctcaagg
     7201 attgatcttg atttgggcac ttatgctgaa tcgcccgcta cctttgtccc cagtacagat
     7261 tttatgggtc aatatggtaa cgacaatcac tttatcttat gcactagggt tcgagtcgcc
     7321 agatccagaa atcatgaagc gcccaccaag agatccaaag caaggaatcc tatctggtta
     7381 tcatttgttt cggatcatct atgtctccct actgatcatg gttcctgcct atttattggc
     7441 tatgcagttt gaagggcaaa gcttacagca aaccattctt ctgcaaaata tcgtcttagc
     7501 tcaagctgtt tacatgatca attgcagaga gttgtccaag ccgtcattga accaaggtct
     7561 tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt gctttattgc aagggatggt
     7621 tatttttcta ccagtcggcc agcagctgat cggtacagta agtttaacga tgacacaaca
     7681 gttattgatt ggagcaaatg tgattcttct attcttagtt gtagaaatag aaaaagggat
     7741 catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga gaatacagtc gctagccgtt
     7801 actataaaaa agttttgaaa gtgagttcag tcaaacgatt gagctcattt ttcgttggaa
     7861 tagaagaaat gtatacaata gggatatcta gagaatctag agggaaattg gaaatcaaga
     7921 tatctgtttt ttcaaaagaa atagtggaaa gatcggttag ttcgataggt aagaagcaga
     7981 cacgttttcc taagtaatct tttaattcct gctgcaagaa cgccttccat gaaggttctc
     8041 cttggaaaat aaaatagaca ttgaacaaat tcggtttgat agaaaaaatc gtttgttgga
     8101 tcagtagaga caagagttgg agaaagtaat ttggttcatt gatttctatt tcttttttta
     8161 gatcgttcag taaattctct ttctgttgat acaagtgtgc atattgctga tcatatgttt
     8221 ctaaaaaacg atggtattga cggtccaatg cttcaatcaa tatcggcgat tcatgatatt
     8281 ttaatatgag gagagccaag ttatcagtca gagatgtttt ttttagttga tcaagagaca
     8341 gctctgaagc tagttcctct acaaacgttt cgaggacctc taaaaatgga gaatgatgca
     8401 ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt ccaactttcc agaaagcaga
     8461 aagaagcaaa agaaagctca gtgtctttaa gataaatacc ttccgttaaa tacaattttt
     8521 tcaatgcaga gtcatacagg gaggagagtg tgctgttggt atggatagaa gaatctaatc
     8581 ggcaattcgc ttttatacgg atcgtattga taaagaacca acatgttccg aaaatctctt
     8641 ctgtttcaac cgctagttca gtttgactga ggtttcttaa aaattgaaaa taatgggatt
     8701 catggatcga ataatcctcg aaaaaatagt tgttatgagt gaaaggaatc agcaatcgat
     8761 gataaaaagt acgaatcgca cgctcttttc caagaataca agctgtatat ttcgtcaaat
     8821 cgatctgcaa gtcgtaactt gctaaacgct gattgatttt agtcacatgt cttttcaatg
     8881 tcccataaga aagcccatgg tcggctagaa aatcagggac agcaactttt tttgtaaaaa
     8941 gcagattttt cagcagttcg acactgactg attgtttgaa aaaaatctcc caaaggtcat
     9001 ttgttaataa attttgctgg ttttgcaaac tgattcctgc ttgatcgctg atgatgtccc
     9061 agccttcggg cagctgagag cgaatagatt gaaggtcaga aaaaatcgtt cgttcggttg
     9121 tccctatgat ttcagctaat tcatttacgg ttatctttgt atgattcagc aattgttcta
     9181 ctaaagaaac atgacgtcga atatcttttt cggtaatgat ttttttcatt agtgaataca
     9241 tcaatcagac tcccttctat tctaagaata cggagaaaat aaacggtggt caagtttcaa
     9301 ctgatttttc aatcattgtt tatttttctg gagcatttac tgatgataag aatttcatca
     9361 tgaacttgaa aaatgatacc actaaaatca aaaaataggt gggcaaacaa taataaacat
     9421 tttaatagta gatggtagca agaccaacac aggcggattg aacatttaag agttgataaa
     9481 aaagaaaaat atcgaagtat ttgattagca actacttgta ttcatttttc tattcttacg
     9541 tggtatcttt gaataaagcg aatcaataag aaagagatgg aagcagatgc taacgaaaaa
     9601 agaactaatc gatcacatca atgaaacata caatttgtct ccagaatata tttttaaaaa
     9661 atttcctgat tattgcgtat tcagacatca aaaaagcaaa aaatggtttg gggtcctgat
     9721 gtctgttcca gaagaaaaaa tctatgatga tgcaagtgac cataggatca tcaatattct
     9781 tgatttgaag gtcgatcctg aacttggcgg aattttgcga ggaaaagagg ggtattttcc
     9841 agcctatcac atgaataaag aacattggat ctctattgat ctacgtaaaa tagaaaagct
     9901 agatgaacta gcagatttga ttgatggaag ttttaaatca acgcaatgaa tgtctaggac
     9961 aatagagaag aggttgtgac agaaatgttt agcttcaaga aataagaagg aatttacgaa
    10021 aattgcttct caaatttttg ggaaatttcg gcttattttc taagaaatta cttctggaac
    10081 accgtttgtt cgaaaaaaga gctgcgacaa gaattttgtc ccagctcttt tttttttatt
    10141 ttcctaaata aaattcccgc atcgtttttg caatcatttt gatcaaagga tgatcttctg
    10201 tgaagctaga agataaataa attgttcgtg tcaagggcaa atctttgatt tcccgcaaga
    10261 cgaaaggatc atttttatcg taagaccaag aaacttttgg gaaaaagcta acagctaatc
    10321 cttgcttgac aatagaacgt aatgtcgcag gatcatctgt cgtcgaactg atcgtcaatt
    10381 cgatactttt cgcttcaagt gctgcatcca gtgtatgacg gaaggcgttg ctctttgaca
    10441 gcatcaagat gggggtgcgt tggatatctt ctaattgcag atgttctttt ttagcaagag
    10501 gatgtttctt aggaatggcg acataaacag gttctttcat gataggaata ttggtcatgc
    10561 caggcttcat ctccgaagtg atgtacagta caggatcggt aatatcatct ggtaaatgat
    10621 gttgcatgat cgttaattgg atatcaggat gcttttccat aaaaattcgg accaagttcg
    10681 gaatagaaac agaggctacc tctaaataca atatgacgga agtaacgatc ttctcattga
    10741 ccgaagacaa taatttgatt ccttcttcta atgtgtctaa tgtcttgact gcatatggat
    10801 aaaattcttc tgcataaacg gtcgtctcaa tgtttctgcc gctatggtaa aacaaaggga
    10861 cacctaattc gttttccaaa tgtttgatcg cacggctcaa ggcaggttga gaaataaata
    10921 attctttggc tgcttgggta acattttttg tttcgaatac ttttttaaaa tactgcagat
    10981 gaagcaattt cataattatc catcccatcc ataccttttt gttatggttt tttctttttt
    11041 tggcattttt tattatagca gaaattgact aagatagcag tggaaaaagt aagtgctgag
    11101 gaggcgaggc tcatgagtac aggtttctta tttcctggac aaggcagtca gaagttaggg
    11161 atgttagcgg atatccctga aatgtacatg gaacgtttag aaaatgtgac ggggtatcgg
    11221 ttagttgata aagaagaaaa ctatcaagat acggttttta tccagctagc attactgaca
    11281 aaagcagtat tttatttaga tgaaatggaa aaaaacggta ttcagcctga tattgcagca
    11341 ggacattcga ttggtgcttt ttgtgcagct gttgcttgtc gatctttgag atttgagaat
    11401 gcagcggaac tagtctatca ccgcgcagtc ttaatgaaag aagcttatcc aagcggttat
    11461 gcaatgggcg tagtagtagg tgtgacgaga agcgaagctg aggaaattgt agcaaaaaca
    11521 tttgatcctg accatccagt ttatctgtca aatgaaaatt gtccgatgca acatacgtta
    11581 tctgggagta tcgaaggttt atccgttacg ttggcagaag ccaaacggca ccaagcgcaa
    11641 acagcgaagc tgctgaaagt ccctgtgcca tctcactgtc tcttgatgaa tgatacggtc
    11701 aagaaattca agccgtatat gcagtctgtc catctatctg atgctagatg tttgtatata
    11761 aaaaatacgg atggaagagc cactcaggac gcagaagaga ttcgtaaaga tttgttggaa
    11821 aacctagctt tccctgtgca gtggaaccaa atgatggact tggcaaaaga attaggaatg
    11881 gatctgacga ttgaatttcc ccccggcaat actttgacaa agctgattca tacaaaattt
    11941 ggtgtagata aagggatccg tacgatcaat ctagaccaac atggtattga tgacgccacg
    12001 tttctatatc aaaaatggag atgactaatt atggaaaaac caaaccgcaa ctgggcttct
    12061 aaacgcgaaa aaaaagcgca gcgattggaa aaaatcagca acttgctcaa tggcaagtat
    12121 gtagaaactg aaaaaatcgt agaagtaatg gaaaaactga ttgctcccgg cgacaaagtt
    12181 gtattagaag gcgacaatca aaaacaagca agttttcttt cagaaagtct tgaaaaagta
    12241 gatgcagaaa aagtgaatga tctgcatttg atcatgtcta gtatctcacg accagaacat
    12301 ttgaatattt ttgaaaaagg aatcgcaagc aagatcgatt tctcttatgc tggtgcacaa
    12361 agcttgcgtg tcgcccaaat gattgaagac ggcacaatga aattaggtga tatccatact
    12421 tatttggaac tttatggacg cttattcatc gatttgattc caaatgttgt tttagtagct
    12481 gcagataaag cagataaaga cggcaacttg tacacaggtt tcaatacgga agaaacacca
    12541 acaatcatcg aagctgcagc tttcaaagat ggtatcgtga tcgttcaagt taacgaagtt
    12601 gtcgattctt taccacgtgt agatatccct ggtgactggg tagacgttgt cgtgaaagca
    12661 gataaacctt atcaattaga agctttattc acacgagatc ctcaaaacat cacagaattg
    12721 caaattctaa tggcaatgat ggcaatcaaa ggaatctacg cagaacacgg cgtacaatct
    12781 ttgaatcacg gtattggatt caatacagca gctatcgaat tgttactgcc aacttatggt
    12841 gaatcactag ggttaaaagg gaaaatcgcg aagaactggg tattgaaccc acatccaacg
    12901 atgattccag ccatcgaatc aggttgggtt gaaagcatcc atagttttgg tggcgaagtt
    12961 ggtatggaaa aatatattgc tgctcgccca gatgtctttt ttgtcggtaa agacggcacg
    13021 atgcgcagta accgaacatt atcacaagct gcaggacaat atgccatcga tatgtttatc
    13081 ggttcaacct tgcaattaga ttacgaagga aactcttcta ccgtaacaaa aggacgttta
    13141 tctggttttg gcggtgcgcc taacatggga cataatcctg gcggtcgtcg tcattctaca
    13201 ccagcatggc aatcacttcg cgacaatgat gatccattag ggaaaggcca aaaattagtt
    13261 gttcaaatgg ttgaaacata tggttcaaat aaaaaaccag tctttgtcga agaattagat
    13321 gcactagctg ttcaaaaaca agcaggctta gccaatgcac cagtgatgat ttatagcgaa
    13381 gatactacac atatcgtgac agaagaagga attgcttatt tgtacaaagc aaaaacaaaa
    13441 gaagaacgac aagcagcaat tgcagcaatt ggcggtgtta cacctcttgg aatgactagc
    13501 acgaaagaat cactagatag cttgcgtgca gccggcatcg tgaaattacc agaagatcta
    13561 ggcatcaaac gtagtgaagc gaaacgttca ttattagctg cacaaaccat cgatgatttg
    13621 atcgaatggt cagatggcct gtatgaacca ccaatgaaat tcagaacttg gtcataaaat
    13681 tggcgagaaa aaccttttgt cgccatttac ccagatggaa gaagaaagac gaataagcag
    13741 aacgtgtatg ctctgtttta caaaaagggg agagatccat ggaacaatta gcttataaat
    13801 atccaacgaa acagccgatc aataagaaaa tccatgtagg agtggtcggt tcaggagatt
    13861 tggaaatctt gatgtttcca acagaaaaaa cagaatctac tgtaaaaatt tgtacaggaa
    13921 gcgatggctt cggcgaagta tggaacaatg tattaacgag attttttgat cgttatccaa
    13981 tttcagcaga tatcgtgatc aatgacttcg gtgctacacc aggagttgtg tacctacgat
    14041 tgacgcaagc aatggaggaa ctttcagatg aaaaatagtt ttgtagaatt aaacgccaga
    14101 gaacgagtga acgctctact tgacaatgga cgtgaattga ttgatccatt cgatcaaatc
    14161 atcgcgccaa gtttaccagc acaaggaatc gtccctgaaa gtgatgacgg gatcgttgtc
    14221 agccgtggca cgatcaatgg caaacaagca gtagtaatcg ctatggaagg aaaattccaa
    14281 ggtggcggta tcggtgaagt ttccggtgca aaaatcgttg ctgcacttga acatgtgtta
    14341 gaagaaaata aaaaaggaaa tgagatcttt ccagtcattg tactagatac aggtggtgtt
    14401 cgtttgcaag aagcaaacta cggactgtta tctatttcag aaatcggaaa tgtaatcatt
    14461 gctctaaaac aatatgtacc agtcatcggt ttgattccag gacgtgtagg atcatttggc
    14521 ggtatgtcga ttacttcagc attaatgagt tatttaatcg caacaaaaaa agcgagagtc
    14581 ggtttgaatg gaccagaagt tattgaacaa gaagcaggcg ttcgagaatt tgattctagt
    14641 gacaaagact tgatttggaa tacattagga tctcgtcaac gggtagaatc aaaaatcatt
    14701 gatgaattag tgactgatag tgtagaagca atcaaagctg ctgtcatcgc agcaatggac
    14761 gaacgaaaag acacgcatcg cagtgaaagc tcagcatttt atttatcatt acttgattct
    14821 ttagatcttt caaaaccaat ggccattgac gattacaatg aagcaattgc taaccatcaa
    14881 gcaaaatcaa ttgacttacc agaagtagca gaaggaaatg aaccagctgc agcaagtgtt
    14941 ggttacgatt ggtttgcggc actaactggc atgaaaaatc caacttcttc tattagtact
    15001 gtgtattatg gtaaatcaag taaattcata gaagaagcca tcgtgacatc aatcgtacct
    15061 gatcctaaaa acccgatgta ccgtgtacga aacggtgaag ttggtttagt tgaaggtttc
    15121 cgcatgggac aaattttgga tcacgtttac gaagaagata aagacaaaaa agtgaaacgg
    15181 ccaatcatcg ttgtgatcga tgtaccaagc caagcatacg gctataacga agaattgatc
    15241 ggtatccatg tggctttggc taatagtgct gctgcttatg caaaactccg tcaagctgga
    15301 catccagtta ttggattgat tgcaggaaat gctatttcag gtgcattttt agcacatgga
    15361 ttacaatcaa gccgcttgat tgctttaaac aataaagaaa ttaccgtcca agcgatgtct
    15421 aaagaaagtg ctgcacgtgt gacaaaacgt tcggttgctg aaatcgaagc agctgctgaa
    15481 aaagttccag ctatcgctta cgatatcaat aattatacaa aactaggagc gatctatcat
    15541 ttcctagaag atatcaccga ccaagctgct tcagaacaaa atgtcagcaa agtcatcgaa
    15601 gcaatcaatg aagccattca agatgtccgc aatacaaatg acacgatgct gacaaatcga
    15661 tacacaaacg aaacagccaa aacattcgga cgtgtcgaaa caaataaagt ccgtgccaaa
    15721 atggacgaag aatgggatgc ataaaaaatg attcaagcac atgatattgt cctttttgat
    15781 aaacatgaaa tagaagggtt caccttgcct gaatgggtcg ttctaactga aaagatgttc
    15841 ggaacagttc gtcgtgcgtc gacggatcac gcagaaatac taccgattgg tttacgtgga
    15901 cctgcccgca atcagagatg gtcaacagaa atctcaaaac atgccgtcaa acaagtcatt
    15961 catccgtggg acatcatcga acaagaatcg tatagacaag agaaaatcat ggattatcct
    16021 gtttatcagc aatttcatgc tgctagaaac atattatcag gatgtaaatg gggaatcggc
    16081 ggcagcttag gatttgaatt atcgactggg attccggcag taaaagaaac cagtgatttt
    16141 gatttgctgc tgtacgcaaa ttcaccgatt gaattgccaa ttcaggtaat tcaatcgcat
    16201 cctgcttttt ttgagcaatt cgatacccaa gtagtgactc ggcaaggcgg tttttcacta
    16261 aaagagtatt tgagagagcc agagaaaaaa attctattga aaacagtcga aggacctaaa
    16321 ttaaccaaag aactttggta ataaagaaaa agagagagaa agagatgttt gaaaacaaaa
    16381 aaatcaaaat caacgtaagt gacttccaga aagtatcagc tgtaacggct gttatgatgc
    16441 aaacgatatt gagctttgct ttggcaaaca gccacagcgc aacaaataca gcttttcttg
    16501 gtacctttta cgtcatcgtt aaatacacag caccaatgtt tatttttgca attgtttata
    16561 atatggtaaa aacaagtcag catttgtctt attgggaatt tttgaaagaa aagttttttg
    16621 aactagtcgt tccgtatctt ctatggacga cggcttatct ttggctattt ccagcagtcc
    16681 aacaacaaac tccgtatacg gatactgcaa gctttttact gaaatatgtc acaggtgatg
    16741 gagcgcctca tttatggtat accgttatga tgctgcagat ccaattgttc atgccgtttt
    16801 ttgtttggct cggttacaaa gtatttgcta ataaaaaata tgtatggcca gtattgattg
    16861 gttcaacgat tttatatgtc gcatggtatc tgttctatca aacacaagta ttgactgggc
    16921 cttatcatga cagctggtac ttattagatc ggtttgttgc tagtttcatg atttatgggg
    16981 tagccgcatt tgtttatcac gaaaaagtct atcaatattt ggacagagta cgttatcttt
    17041 tcttacctgt tgccttagtt atcgcgttct tttctgtccg ttcattgctc gcacatccag
    17101 gagacttgag ctttgcgaat gcaccttact tgaatacgat ccaaagttta tacagtttag
    17161 tcatcatctt tgctgtcttc ataggcgctt ccaaaatgat tgtaaacgat tcgccaaaat
    17221 tgccattgtt caaatggttg tctgtttatg cttatcgtac gtatttagcc aatgtatttg
    17281 ttttccaagt actgttatta ttgtttaaag atagttggct gcagttacct tcaggagtca
    17341 tgattcttgt agcttatcta atgacggcaa gttgtgcatt tgctttaagc tggttacttc
    17401 atatcatttg ggtagctatc aaaaaaggtt tcagcaagta atagtaggaa gaaggagtga
    17461 gtgtcatgat aaatgacgaa cgaaaaaaag cagtgactca gcaactcgct gcgttggtag
    17521 aggaagcatt gatcgcagaa gtaacattaa gtccaaaacc tggattagtg gatgcactgg
    17581 atgcgggtgc acattccgat atggactatg ctttatttct taagtctgct aagacgctaa
    17641 ctcctttttt tgaagaaatg gctcaaatag catggcatca tgctattgat caggagttaa
    17701 gagaaagaat tgctgagatt gggcgaaaag cggaaaaggc aatgctgatt gctacaaatg
    17761 gcatcaatac ccataaagga gcgatttggg caatggggct attgatcagt gtcattgcac
    17821 aaaaaatcag tatggaacag cctttttctt tatcgaaaat acttgatgat gtcagcaagc
    17881 ttgctgcttt tcctgataca aaatatcgtc ataaaaaaac cactcatggt catagggtga
    17941 agcaaaaata tggcgtaaat ggtgcatatg aagaagcgat attagggtat ccgcatatca
    18001 ggcttgcaat tcaagcagct aaggagctag ataatgattc atatgtacag aaacaaatgc
    18061 atatgttgtt agttttgatg agttcgctag atgacacatg cgtattgcat cgcagtgatc
    18121 agaaaacgtt agtggaaatg caaaaacttg ctcatgctgc tagtcaaagt gctttaccaa
    18181 atcgtccatt tacagcactg ttgcactttt gtcaggaaaa ccaaatctct cctggaggaa
    18241 gcgcagatct cttgtctgca agcttgttca tgctgaagat agaggaatta tggactgccg
    18301 acacatacat ttgtccgatg ttactcaatg actaaataag acaaaattgc taaaaaaaca
    18361 gtttatgcat ctggttattg actcttcctt ttagcaaggc tatcataaaa atagtttttt
    18421 gtaataaaag ataacgagga agacaatgaa aaaaatcaat tatttagcaa tcattttgtt
    18481 agtgggccta tgtttgtttg ctctgccgac ccaaggattc gctgtcgcgg gtggtcatgg
    18541 tggaggtaca tctagcggtc gaagttcggg cagaggttca tttggtggtc gttcatatgg
    18601 gggcagttat tttggcagtc gttcttacgg cagagattct tatgggtatt atggtccagg
    18661 tacaggtcct tctagttatg agcaaacggg atttcttgca ttagctgttt tgtcagtagg
    18721 aggggtttct gctttttcta tcgtcaagaa taaaagacaa aagaaaaggc tgaaagcgga
    18781 gatattgtcc cgcatgtcag gaacggctaa agaaaagaaa gcacagtttc tggaaatcca
    18841 aaagatattc atgaagatcc aatccgcttg ggaagaagaa actcctgaaa aagccagaag
    18901 ctgttataca gacaagctgt ttgccgaaca tcagaaagtt ctgcaaaaaa acaaagcaga
    18961 cggtgtaaga aatcatacta aaaaaataac tattcaggaa gtaggaaatt atcgacagat
    19021 ccacgaaaat agttttgcca tccgaattga tttcagatgc tgtgattatt cagtcgatcg
    19081 aaaagatggg cgattgatct caggaagtaa gacacaacga cagtattttt ctcagatatg
    19141 gtattttgat ttttctgaga aagaaaaaag atggcaagcc gattttattc agccgattat
    19201 gctggattga caggaaatat ttatgaacgg agttgtgaca aaagccttgt cgcagctcct
    19261 ttttctgaat aaacggtgtg ccagagatag cctatcggca ataaggcacg attttgtaaa
    19321 atatgggaag cgatttttca aaatcgcttc ttatcgctca aggctgaaca tctctgtcac
    19381 aacctctcca ctaaatggtg tttaaaagct tgcatctgcg ataataagaa caaccaaacg
    19441 aaaaatagga agcactattt tagtttagtt gttcttatta cttgatgcag gacagctttt
    19501 tcacaacctc ttttttgtat agatgggcaa aatttttgta ataataggcg tatgtctttt
    19561 tcgttgagga gatgaaaaag catatggctt attttttgtt tgctttgctt actttctttc
    19621 cacttcaagt ggcagaaata tctacttttt attatgtaag cggaaccata tacttgaaat
    19681 cagaaggaga tcatttctat gaaaacaaga acaattgaaa tacttcacag aatcatgaca
    19741 gcccaatatc cagtaaaagc aaaacatcta gcaagtgagt tcaatgtcag tcagcggacg
    19801 atccgtcagg aagttcttga agcgaatatc tggttgagca gtaaaaagct tccggagatc
    19861 cgtacaattc gcaacaaagg ttttttgttg aagctggaaa aggaaaagca agctcgatta
    19921 gaaactgaaa taaaagatgt gaaagacggc tttttgaatc ggcgcgaacg tatttttgat
    19981 ctagttttag ccgttgctta cagccgaatc ccgatccatc taaacaaaaa agaagaagaa
    20041 tttcaaatct ctaaaagtac attagatgaa gatatgagac ggttacgagc aacactggtc
    20101 agttatggga tagagatcgt cagttatgga aagcaaggac tggtttataa aggttcagaa
    20161 cgatctatcc gtacgatgat ctatgatttt atcaatcaga atctgggaat gaccgacttc
    20221 tttatgaatc aagatgcaca attaacaccg gctcaaaaaa tctttctcca ttattttcct
    20281 ttggtggaaa tcaaaaaaat acaggagatc tattccaata agttgttgaa acaagaagat
    20341 gatatttata aaaaacaaat cctgcttttt acattgatct ggatccagcg aatcaggaaa
    20401 catgaattta tctctgccat taattggaaa aatacagaaa taaaagacag tagcttttct
    20461 gcatttattg accacatgat cgaggaattt caattggaca atgtcccgca agtagaaaga
    20521 aattatcttc gtttcactat tgagacgttc aatgctagag atatctccaa ttcaatcgag
    20581 tgggtacaag ctcaattact aacgatccaa ttgatccaat ttgtagagaa cgagacacac
    20641 gtacctttcc atctaaagga agaagcttta ttcgaaaatc tctataagca catggctgca
    20701 ctgattgtaa gaatcaagaa tcatatccaa gtcatgaatc cattgaaaga aaacatccga
    20761 acaaattatt tcccaatcta ccgcgcagtc actcattttg ttcctacgat cgaagaagta
    20821 attggtggca agatactgga ggacgagata gcttttcttg tcatccactt ttcgacgatc
    20881 gccagtgtaa tcaagcgaga cttgaattat gtttataaat cagtcgttgt ctgtaaccat
    20941 gggatggcta ctggaaatct gttagcagag aatctgaaag aaaagtttcc tcagatcgag
    21001 attactgcag ttttaagttc aaaagaaatc gaattagtag ataaattgga tgtcaatctg
    21061 gttttcagca cctttcaatt gaattatcga aataagccgc ttttagttgt cgatccgatt
    21121 ttaactgaga cgaatatctt ggttatagaa gactttttga aaagaaatga agaactacag
    21181 cggttagagc ctaacagtca tgaatcgacg gagttattca ctcaaataat agatgtcatg
    21241 aaagaaagtg gtggccgagt agatcgaatc atctatggca agttagaaaa attattcgaa
    21301 acaaacaatt tagaaatcaa caaaagggag atccagccta tgttaaaaga tattttgacg
    21361 gacagcaata tcatgatcaa agaaaaagcc actacttggg aggaatcgat tgaactagct
    21421 gctagaccat tggttaaaga aaacattgtc gaagagcgct atgtcgaagc gatgattcgt
    21481 gcagtgaatg attacggacc gtatatcgtg attggcaaac acatggcact agcccatgcc
    21541 cgaccagaag atggggtcaa taaattagga gtcagcgtag ctacgattga gcaaccaatc
    21601 gatttcggta atcctgaaat ggatccagta aagattattt tttgtttggc agcagtagac
    21661 tcctactcgc atctgaacat catgaaagaa ctaattgaat tgatcaacaa cgaagagaag
    21721 ttggatcgat tgattgaatg cacaaatatc gaagatttca aacaactgct tttttcagta
    21781 gttgagaaat aataaatgat acgggggaga acaaagatga acaaattgaa cattttattc
    21841 gtatgcggtg caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg
    21901 aacaaattag gagtagaagc caaactggac cattgtgata tttcttccgc agtttccatg
    21961 aaaccagatg tgatcatcac agcagaaaat tttcgttcgc agttcgaaaa gttcgcagtt
    22021 gatcctaaaa cggcgatgat ctatttgaaa aatattgttt caaaaaatga aattgaagaa
    22081 aaaatcaccc cagttctgca agataaggga gttatctaaa tacaaaaaga aaagagggta
    22141 aaaacatggg cgttatcaat tttatcatcg aaaatatttt gactcaggca tcgatcacca
    22201 ttgcattgat tgctatgttg ggtctactac tgcagaagaa atcagcagga caagtcatct
    22261 ctggcacgtt aaagacactc ctaggatttc aagtgttgag tgctggttcg agcatcatcg
    22321 ttggaagttt aacttatttc gggaaaatct ttactgaagg atttcatatg caagggatca
    22381 tcccttctat tgaatctatc aatggtcaag caatgaatga tttgggttta ggtagagata
    22441 ttgcactgac gttcttagcc atttttgtat tcaatattat tttggctcgt ttcacaaaat
    22501 ggaagtacat ttttttaact ggtcaagcaa ttttatggat ggcaacgatg acgacagtct
    22561 ttggctattt tgccggtctg cgagggatcg tattgatctt ggtcggtggt tttatcggag
    22621 cttgtttcgc cattgctatg ccggcagtag cgcaaccgat cattcgtaaa atcactggat
    22681 caaatgatat tgctttaggt catttttgta cgatcgggta cttatttgaa gccggtgttg
    22741 ctaagctttt tggtgagaaa ggcgaaaata aaaagtcgat cgaagatatc aagttgccga
    22801 cacatttcga atttctgcaa gatacttatt tgtctgtcat ggtcgtgatg gtcccgctat
    22861 atatcatcac tgttttattt gcaggcgaac cattcgcttc tgaattatca ggagatcaaa
    22921 actatatcat gtttgccttt ttacaagcca tccaattcgt agtgggtgtg tatgtattgc
    22981 tagctggtgt acgtctactg ttaggtgaaa tcgtgccggc atttcgaggg atagctatga
    23041 aattagttcc tgatgcgatc cctgccttag attgtccagt tttctttcct tatagcccaa
    23101 atgctgtcat tttaggtttc attacgacaa cgattggtac gattattgcc atgtttactt
    23161 tgcccatgtt cggcttagcg atgattttac ctggtatgct aaccaatttc tttgccggag
    23221 ggacagcagg gatatttggc aacgcagttg gcggacgccg aggagcgatc attggcggta
    23281 ttgctcatgg tttctttatt acattattgc ccgctttgct agtaacgatc tttaattcga
    23341 tgggattcaa caatgctaca gcaaccgatg tagatacagt agccgcagca ctcttatatg
    23401 cctggatctt aagcccagtt ttgaaagcat tttaattgaa agtagggaaa atgatgtttg
    23461 gattttttaa gaaaaaagaa aaagaaccga aaaaagttct ggcaaaagaa gaaaaagccc
    23521 tatcgccaga agcgaccgaa aagattcaat cagagattac acaattgaaa caagagattt
    23581 ccactaccca agataagcat aaattagcga aactttatga acaagttgga cttaaatttt
    23641 ctgaattgta tgtgaacgat caagccatcc aatatttaga aaagagtttg gaaaataaac
    23701 agacgatcgg agatgggtat aaaaaactga tgagcctcta taaccagaag cgtgcagatg
    23761 ctgctcgtgc tggtgatgat caagggatcg attactacat gggcaaaatg gatgagatgc
    23821 gacaaattgc aaaacaagta acgatcaaag gaaacaaata aaaagataag gtggcaataa
    23881 aagatgtata caacattgaa agaagtaaca aaaacagcag aagagttgaa ttttacagtt
    23941 ggagctttta atacgcataa cctagaaatg cttcctgcta tgctaggcgc agcaaaagaa
    24001 atgggttcgc cgatcattat tcaaacaagt gtcgataccg ctaaatatat cggcatgcat
    24061 gtgttagtca atgtaatgac agctattgca gaagaagaaa tggttgatgc agtcttgcac
    24121 ttagaccacg cccgagactt tgatgatatc aaagaagcaa tcgacagcgg atatacttct
    24181 gtcatgtatg atgggtccca tctgcctttc aaagaaaata tcctgaagac aaaagcagta
    24241 gtagaatatg cccatgcgca cggtgtttcc gtagaaggag aacttggaac aatcggcggt
    24301 acagaagaag gaatccatgt cgctgaaaat gacaaggtat atacaagacc ggaagacgca
    24361 gtgaaatttg tagaagctac aggcgtggat gcattagcta ttgctatcgg tacgaatcat
    24421 ggacaattca aatcaaaaac agaagtaaac attcccttgc tgaaagaaat cgatgcagta
    24481 gttgatgtcc ccctagttat ccatggagga acaggtgtga aagaagaaga ttatcctgaa
    24541 ttgatcaata acgggatccg caaattcaat gttggaacgg agcttttagt caactggacg
    24601 aaagtagcta aagaaacctt tagtgaaaca gaaatcaaca agtctcttcg ccataatgtg
    24661 attccagcaa atgaagcagt aaaagaaatt gtcaagcata agattggact gtttatgaac
    24721 ttggaaagtc caattaactt aggaaataaa tgagatgaaa aagtatttca ttcttctggc
    24781 tggtagtcct ggtacaggaa aaacatatct tatgaataaa ctacgggaac gatttccaga
    24841 tatgtatgcc atcacgctcg atgaaatcaa ggaatactat gcagaatcca tcgggtttaa
    24901 caatttggaa gaacgggcgg aacaagaaag gacgaaagtt tatcctttct attacaaagc
    24961 acttgatttg tatatggaag caggaaagaa agtgattgtg tccgagtatc cttttagtga
    25021 taagcaaaaa gcacgactcc aagaattagc agatagatac ggatatcagg tgatcacgat
    25081 tcgcctaaca gcagactttg aagtgttatg gcaacgtcgg tacaaacgtg atcgtgaatc
    25141 agatcgtcat ttgagctata tcatggatca ttatcactat ggtgacgtat tggaagatcg
    25201 aacgaaaggg accaatcata ttacgaaaga agcattcaaa aagatcatcg ccgaccgaaa
    25261 atatgacaaa ttccagttag gaacactata tgaatttgat gtaacagact atactcaagt
    25321 ggattacact ccactattgg ataaattaga aaaagaaatc aatgacaata agtaacaaag
    25381 aaatggctta atatcgaaaa attggctttt gaacgctgtt taccgagagg tcgtgagcct
    25441 gtcgttcagc tccgagtcac aaggagcaat ttcaaaaaaa cagcttctca tgtttgatga
    25501 atattgtgac ttgtgaccga ggagttggta ggagaacacc gtttagtggg gaggttgtga
    25561 aaaagctgtc ctgcctcaat taataagaac agccagacaa gaatagctcg tcatattttt
    25621 cgtttggtgg ggcttattac cgcagatgca agcttttgaa cgccatttat tcacagcccc
    25681 agagaagtcg aatgtctttt gttctcgagt tttttaacat tcttttattt attgcagatt
    25741 tcattatatt tatttgccgt aattaaacca gtcttctata cttgattaca tagtcataca
    25801 tttgctaaaa aagaagggaa gagaatgaac aagaagttgg ctcaatacaa aagactactc
    25861 gcactttctc aagctggtct atattatgga aaagatgtat ttgataaaga gcgttttgag
    25921 gagatccgga cgatttcatt ggaactgatc agtgaaatag gaaaagaaac tttcgaagaa
    25981 ataaaagcgc tcacaacgat tggagaagga catccaacac caaaagtaga tgttcgtgcc
    26041 tatatcaaaa aagatggcaa ggtcctacta atcgaagata aacggacgaa agaatggtca
    26101 cttcctggtg gatttgctga aataggattg tctcctgaag aaaatgtgcg taaagaagtc
    26161 tatgaagaaa caggattaac agtagaaact actcagttgc gagcagtttt tgatacgaat
    26221 aaacaaaaag atattccaca gctttttcag tattataaac ttgtttttgc ttgtgcgatc
    26281 cacggtgaag aggccaagtt tattgagaat aatgaaactt ccaacatggg attttttagt
    26341 atagaagagt tgccaaagct atcagaaaag cgaacaacga agaaacagtt acttatttta
    26401 gaaaacaaaa accagatcta ttgtgattga gctttttcgt aaaagaagaa aagaatatac
    26461 tttactagtt attaaaaaag aacttgattt ttttattgaa aatagttgca tttgcaacaa
    26521 aaagaggtga actagtctta caaacaaaat aaaggtggga tgaaatggct gaaatccttc
    26581 gagacatcgg aatgattgcc cgagcgctag attctattag caatatcgaa ttcaaagaag
    26641 tcgatttaac aagaggacag tatctttatt tagtacgtat ttgcgaaaat cctggaatca
    26701 tccaagaaaa actagcagaa atgatcaaag tagatcgtac aacgactgcc cgagcaatca
    26761 agaaattaga atccaatggg atgatcgaac gtttggaaga taaagaaaat aaaaaaatca
    26821 agaaactcta tccgactaaa aaaggagcag aaatctatcc tttcatcatt cgagaaaaca
    26881 actactccaa tgcagtcgca ttaaatggtc tttctgacga agaagcaaaa caattggaat
    26941 atcttttaaa taaagtatgc aagaatgtta gcgaagattg gaattttgtc aaaaaaggca
    27001 ataaaaggat ctattgaaag gaagaagccg tgaataaata cgaaattcga aaaatgacgc
    27061 cttcagatgt agacttacta caacaaatta gtatcgaaac atttacagat acatttggaa
    27121 aagataattc accggagcat ttagctgatt acttgacaca agcttatgca ctagaaaaac
    27181 ttacagaaga gctaaaagat aaagaaaccg attttttcct agtgagtatc gatcaagagc
    27241 ctgcgggtta cctgaaacta aatacaggaa cgagtcaatc tgaggaaatg gctgatcatt
    27301 ttttagaaat cgagcgaatc tatgtacgaa aaaaatttaa acatcaaggt atcggtacat
    27361 ttttactaga tacagctatt gaaaaagcaa aagaaaagca aaaaacagcc atttggctcg
    27421 gtgtttggga acacaacgat gttgcgctcg ctttttatcg taaaaaagga tttatcaaat
    27481 acagcgaaca tgttttttat atgggagatg atccccaaac agactatcta ttcatcaagc
    27541 aattattcta atcaagaggt gtgaacagat gaaaaaagta atagtggata ccaatttctg
    27601 ggaagtattt cctgaagcaa ccatcgaaat cttatccgta aatggtatcg ataaccacgt
    27661 aacaaaagaa agtgaagaaa cgtatcatca attattgaat gaggcagcaa aagaagcaag
    27721 aaattacctt acagaagaaa cattcagcca aaacgaagtc attgctcagt ggcgtcaggc
    27781 atttaccaca ttcaagacta aaaaaggtgc acgttcttct attgaagctt tactgaaacg
    27841 tgcaaatcaa gaacgggaat ttttcccaat caatccgctt gtagatatct ataatagtat
    27901 ttccttgtct tttggcgtac cttgcggcgg agaagatatc gaaaagatcg caggaaatct
    27961 gcatttagga aaagcccaag gtggcgaatc attcctgcct ttaggtgcag attccgatgc
    28021 tccggcactt ccggaagaaa tcatctacta tgatgaacaa ggagcgatct gtcgctgtct
    28081 caactggcgt gaagcccaaa gaacgatgct gacggaagaa acacaagcag cggtgctggt
    28141 cattgaatcc atcaatgctg aacaagcggc acgtggcgca caggcaatcg aagcgttgaa
    28201 ggaaaaaatc gatcaaacat ttggcgtaac tagtgttaga cacacactaa ccaaagatga
    28261 atcagaagca gaattataaa agaatcgaaa taa
//
LOCUS       DAXWLB010000016        28250 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXWLB010000016 DAXWLB010000000
VERSION     DAXWLB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.016"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(282..1364)
                     /locus_tag="ACGE4H_000559"
     CDS             complement(282..1364)
                     /locus_tag="ACGE4H_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8363999.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            1506..2957
                     /locus_tag="ACGE4H_000560"
     CDS             1506..2957
                     /locus_tag="ACGE4H_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8364000.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            2950..3672
                     /locus_tag="ACGE4H_000561"
     CDS             2950..3672
                     /locus_tag="ACGE4H_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8364001.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            3692..4843
                     /locus_tag="ACGE4H_000562"
     CDS             3692..4843
                     /locus_tag="ACGE4H_000562"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8364002.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(5191..5466)
                     /locus_tag="ACGE4H_000563"
     CDS             complement(5191..5466)
                     /locus_tag="ACGE4H_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFF8364003.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            5574..6338
                     /locus_tag="ACGE4H_000564"
     CDS             5574..6338
                     /locus_tag="ACGE4H_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFF8364004.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            6462..6965
                     /locus_tag="ACGE4H_000565"
     CDS             6462..6965
                     /locus_tag="ACGE4H_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8364005.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            7230..7877
                     /locus_tag="ACGE4H_000566"
     CDS             7230..7877
                     /locus_tag="ACGE4H_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFF8364006.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     regulatory      7920..7987
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            8038..9306
                     /locus_tag="ACGE4H_000567"
     CDS             8038..9306
                     /locus_tag="ACGE4H_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFF8364007.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(9483..10685)
                     /locus_tag="ACGE4H_000568"
     CDS             complement(9483..10685)
                     /locus_tag="ACGE4H_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8364008.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            11212..11937
                     /locus_tag="ACGE4H_000569"
     CDS             11212..11937
                     /locus_tag="ACGE4H_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFF8364009.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            11927..12601
                     /locus_tag="ACGE4H_000570"
     CDS             11927..12601
                     /locus_tag="ACGE4H_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFF8364010.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(12889..13173)
                     /locus_tag="ACGE4H_000571"
     CDS             complement(12889..13173)
                     /locus_tag="ACGE4H_000571"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFF8364011.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(13467..14774)
                     /locus_tag="ACGE4H_000572"
     CDS             complement(13467..14774)
                     /locus_tag="ACGE4H_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFF8364012.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            15263..16369
                     /locus_tag="ACGE4H_000573"
     CDS             15263..16369
                     /locus_tag="ACGE4H_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364013.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            16960..17160
                     /locus_tag="ACGE4H_000574"
     CDS             16960..17160
                     /locus_tag="ACGE4H_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8364014.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            17307..20096
                     /locus_tag="ACGE4H_000575"
     CDS             17307..20096
                     /locus_tag="ACGE4H_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFF8364015.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            20143..20670
                     /locus_tag="ACGE4H_000576"
     CDS             20143..20670
                     /locus_tag="ACGE4H_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFF8364016.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            20691..21881
                     /locus_tag="ACGE4H_000577"
     CDS             20691..21881
                     /locus_tag="ACGE4H_000577"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8364017.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            21921..23219
                     /gene="asnS"
                     /locus_tag="ACGE4H_000578"
     CDS             21921..23219
                     /gene="asnS"
                     /locus_tag="ACGE4H_000578"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFF8364018.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            24153..24827
                     /locus_tag="ACGE4H_000579"
     CDS             24153..24827
                     /locus_tag="ACGE4H_000579"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8364019.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            24824..25165
                     /locus_tag="ACGE4H_000580"
     CDS             24824..25165
                     /locus_tag="ACGE4H_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364020.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            25195..25554
                     /locus_tag="ACGE4H_000581"
     CDS             25195..25554
                     /locus_tag="ACGE4H_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFF8364021.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            25814..25936
                     /locus_tag="ACGE4H_000582"
     CDS             25814..25936
                     /locus_tag="ACGE4H_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364022.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            26078..28159
                     /locus_tag="ACGE4H_000583"
     CDS             26078..28159
                     /locus_tag="ACGE4H_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8364023.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         9213 a         4929 c         5894 g         8214 t
ORIGIN      
        1 cgctcataat tcaatcaact tcatagaaat ttcatagaaa taaccgattg gaggatcaaa
       61 aaagacgctt aatgttaaaa agaagaatcc agctaaaaat acttcagtat aaagcatact
      121 tagcacctcc gttctctaaa ataagatgag caacggatat ttccttgaaa tcataatttt
      181 agcgcaaaaa aaagaagtgg aatgtcaatt ttttccactt cttttttttt accattgagc
      241 cgatccataa aaaagctgac tgccatggta taaggggcta actatccctg tgtcacttgt
      301 tctaactctt tcattttctg gacaaccagt gctggtgtga tcgtttcttt catgaagtgg
      361 atcatttcat caggtaaaca tgcacgttca gctacggctt gatagtcact ttctgttaaa
      421 tgtaaaccca tatcagctaa actcgttggt aagttcaagt gatgataaaa cggcagtagg
      481 tgttcgattt ctgctaattt attttcgatc atcagctgga caaggatacc gtatgcaact
      541 ttgtttccat gaagttgctg atggctatca gggagtacgg tcatcgcatc gtgaatggaa
      601 tgagctccag ctgttcggcc gtagtcatcg ccaaacccac caaccatacc agccaataaa
      661 atgtttgttt ccacgacatc gataaaagct tgattgattt tttgctggtc catcgcttcc
      721 attgcctctg cacttttctc taaaaggatt tcgcggcatc gtaacgctgc aaattccgct
      781 acttggactt cgataggctt gatagttaac tgagagatga ttgcgtgaga ttcataccat
      841 tttgctaagg tgtcaccaat accggcaatc atcatctcgc gaggggagtg caaaatgatc
      901 tttggatcga tcaacactaa atcatttgct ttagggaaca tatcatagcg aagcatcact
      961 ccttcttcat tatagaccac actaagtgga gtataagctg cgcacgtcgc agctaaggta
     1021 ggtaagatga tgattggtaa gtttgtctta taggcaatca attttcctag atctgctact
     1081 ttcccgccgc cgactgcaat aataccattg aaatccttct ttttgcataa agaaacaaaa
     1141 ctgtcagcaa gtggatcggt acattctcca ccataatatt caaaaatttt tgttattgat
     1201 aaatctggaa aaacatcttt tactgctttc caagatgttt ttccatggag aatcaaaacg
     1261 ttgtcaatat gccttttgta aaggtgttca gggagtgaat cccatgcgtt ttcttttatt
     1321 tcatactctt gaggggcacc gcgaacaatt aaattttttg tcatataaaa aacttcttcc
     1381 attatttttt taaattaatc tttttttatt ataatagtta tgctagtata ttagcaagtt
     1441 atttatgaat atttattctt ttatagcttt tctgattaca ttattattca aaggtggcca
     1501 aatggatgaa aaaaggaata aacatattat tagtggcgct gatgtttcta ctggtttttt
     1561 taccagtaaa agtacaaggt gcagaaaaag atgaaattta cgagcggata aaacaaagtg
     1621 gagaattagt cgttggccta tctgcagact atgcgcccta tgaattccat gcgaatgtgg
     1681 atggtaagga taaaatcgtt ggttttgatg tatccattgc cgaaaagatc gcaaaggata
     1741 tgggtgtgaa actaaaagta gaagaacttg gttttgatgc attattaggt gcgttgaaaa
     1801 ctggcaagat cgatttagtt atatctggta tggcggtgac tgaagaacgt ttacaagaag
     1861 ttgacttttc tgacccatat ttcgtggtac agcaaaaagt actcgttcgt aaagaggaca
     1921 aaaataaatt taagacgaca gccgactttt ctggcttagc cgttggggcg caaaagcaaa
     1981 ccacacaaga ggagttagtg aaaaatgaac tgactggtgc agagttgacc tccctgcaaa
     2041 aagttcctga tctgatcaat aatttgctga atgaaaaagt tgaaggtgtt gtgctggaac
     2101 aaccagttgc agaagcatat gcccaacaga ataagaaagt agctttgact ccagtaaaat
     2161 ttgcgcaagg cgaaaaaacc acagctattg cgatgtcaaa aaatacacca gaactgaaaa
     2221 atcatgtcaa tgcgtcaatc aaaacgatca atgacgacgg cttgctgaaa gattatcaaa
     2281 aacaagcgaa tcaattgatg ttccaagatg atcagtcctt tcttcaaaaa tacggtccgt
     2341 attatttgat tggaacaaaa tacactattt tattagcttt tgtgggtgta ttgtttggaa
     2401 tggtctttgg tagtttgctt gcactgatga aattgggtaa gaataaattt ttaaaggcat
     2461 tagccattat ttatatcgaa tacgtacggg gaacacctct cttagttcaa atctttatcg
     2521 tttacttcgg aacaggagta ctgggattgg atctatcgaa actggtagca ggttgtattg
     2581 ctgtctcgtt gaatagtgct gcttatgtag ccgaaattgt ccgtgccggg atcaatgcgg
     2641 tcaacaaagg acagatggaa gctgcacgtt ctttaggaat ggaccagaaa gaagccatgc
     2701 gtttcatcat cttcccacaa gcaatcaaaa atattcttcc agctctagga aatgagtttg
     2761 tcacgatcat caaagagtcc tcagttgttt cggtcattgg ggtatcggaa ttgatctttc
     2821 aaacgggcgt ggtacaaggt gctagcttca aaccattcct accatatgtg ttggtctctt
     2881 tgatttattt cgtgttgacc ttcagtattt cccgtatatt agctttagtt gaaaggagaa
     2941 tgggtctgaa tgcttaatgt aaaaaaactg tcaaaaagtt ttggcgagaa tgaagtctta
     3001 aaatcaattg atctttctgt gaaagaagga gaagtagtgg tgatcattgg cccttcagga
     3061 agtgggaaaa gtacaattct acgttgtatg aatctgctgg aagaaccaac ttctggtgaa
     3121 atctttttta agggacaaaa cattacagcc cctgacagcg atatcaatca aattcgtcaa
     3181 aaaatgggaa tggtatttca aagttttaat ttgtttcccc atatgaatgt actcgaaaat
     3241 ttgaccatca cacctgtaaa aatcaaaaaa gaagacccta aaaaagcaaa agaacatgct
     3301 ctatcattat tgaaccaagt tggtctaaag gaaaaagtgg acagttatcc taccagttta
     3361 tctgggggac agcaacaacg ggtagcaatt gctcgagcat tagcaatgaa tcctgatgtg
     3421 atgcttttcg atgaacctac atctgcactt gatcctgaaa tggtcggtga agtgttggca
     3481 gtcatgcagg atttagctaa aaaagggatg acgatggtcg tggtcactca tgaaatgggt
     3541 tttgctaaag aagttgccga tcgtgtgatt tttatggctg acggcatcat tcaagaagag
     3601 ggaacaccga aagagatttt tgaaaatccg caaaatagtc gaacacagga tttcttgaac
     3661 aaagtgttgt aacaaagatg gaggaagtaa gatggacaaa agtaagattg cacgccggtt
     3721 tcagcaacca gcagaaaatc tgttgatgaa tattgctatt ttggcaaagg aaaaagagga
     3781 tattatcgat ttatcgattg gtgatcctga cttgatcact gatccgacga ttattgaagc
     3841 agcttataca gatgtaaaaa atggtgctac taattatact gcgcctgatg gcagcaaaga
     3901 attcttgtca acggtcgtag atttttatca aaagaaatat ggattgagcg ttgaatgcaa
     3961 ccaagttcga gctacagtcg gagcaatgca cgggatgtat ctagttttac aggcattact
     4021 agatccagag gatgaggtca tcatccatga accttatttt tcaccatatc gggaacaagt
     4081 cattttatct ggaggcaagc ctgtttttgt tcctacctat gaagaaaagg gcttccagtt
     4141 ggatgtggaa gatttaaaag cagcgattac ggatcgtaca aaggtgatcg tcatcaattc
     4201 gccgaataat cctacgggag ctgttttttc tgaaagcacg ttgaaaaaaa tcgctgaagt
     4261 cgcaatcgaa aatgaactct atatcatttc cgatgagatt tatgaagact ttgcctttaa
     4321 agagaagttc gtaccgatgg cgcgatttgc gcccgataat acgatcactg tcagtggtgt
     4381 atcgaagggg tttgcgatga ctggctggcg cttagggtac atgatcgcac cagcatacat
     4441 caatcaaatt gctgggttga tcaatgaaaa catcacttat tccgcaccga ctgcttctca
     4501 aagaggtgca atctacgcct taaagcacta tgaacagctt gtaccgccag ttgttgaagt
     4561 attcaaagaa cgattagctt atattgagaa aagagtggca gaaatccctt acttatcttt
     4621 atcttctgtt tctggaagca tgtatgcttt tattaatatc agtaagagtg ggctcgattc
     4681 agtaacgttt gtggaagagg ttttagaaaa aagcggcgta ctttttgtcc ctggaaaagc
     4741 gtttggggaa acgatcgggg acaactatat tcgactagca gcgactcagc cattggaaat
     4801 cttaggtgaa gcctttgatc gtttagcaaa actaaatttt tagataaaac tgtatatttg
     4861 aaaacagcta tgggtatcca ttagaacaaa gagggaaaat gatcctttcg agagttggct
     4921 tgttgactgg gcagcgtcag gatttgacaa tgaaaaagtg gtagtggaaa atcaaagtcg
     4981 attttccact accataaaat attatagact ccattttatt gaaccaaaat ttttgaagtc
     5041 ctcccgcttt tacgtatgcc tatcactggt tactaaactc gctaataaga atgttttttt
     5101 gtcgatctag gatggagaga ggggcaaact ttttcgatct acatacagac agtccgattt
     5161 ttctgacatt tcctattcat ttctagaagg tcagcctatt acaacaaact tttttctgtc
     5221 ctccatcatc ggattttcct gaaggtctac atattggaca cgtccgctgg ggcttggtga
     5281 tcgtttgact tcttccacga attttttgat tgcctcatcg tcgcccgcag cttcgatcgt
     5341 gacagagccg tcatcttcat ttttcactgt tccggtgatc cctaattgat ccgctacgat
     5401 ttttgtcata tagcgaaaac caacgctttg tacacgtcct tgcacgttca tcttgatttt
     5461 gttcatttta ttcaccgttc tttcttttat ttttaaatta atgataccga cttttttgct
     5521 actgaacaag tgcaatgatc taatccatgg tataatcagt tgaggcgaaa aatgtgaaag
     5581 agatccaatc agcaaaaaat ccaagaatca aagaattaaa aaaattacat aagaaaaaat
     5641 atcgtgaaga aaaaggggaa tatctgcttg aagggtttca cctgatcgag gaggctgttg
     5701 cctcaaatgc gaaggtcaac gaacttttta ttacgcaacg cggtctgact gagtgggcta
     5761 gttggcttga agaaaatcag gcgctttctg ttacactagt atcagaagaa gtgatggcaa
     5821 ctgtttcaga tctaccaact ccgcaaggga tcatggcagt ggtggaagct gaaaaaagag
     5881 agataccgaa ttttgaaggt ggttggttgt tgctcgacaa cgtacaagat cccggcaatg
     5941 tagggacaat gatccgcaca gccgatgctt ttggtctatc ttgtgttgta ctaggtaagg
     6001 gaacggcgga tgtctacagt accaaagtat taagaagcat gcaggggagt aactatcatt
     6061 taccgatcat ccaagaggat ctaacaaaac tattgcctat ccttaaagag caaggattta
     6121 taacctatgg aacagaactg aatgaagaag cagtgtccct gaatgatatc cagacaacag
     6181 aaaattatgc gatcgtgatg gggaatgaag gtcaaggcgt acgtccagaa gtgctggcgc
     6241 taacggataa aaatgtgtat ataccgattc aaggaaatgc ggagtcttta aatgtaggag
     6301 ttgcagcggg catcatgctt tatcacttct ccgtatagtt aaggaactat taagctttct
     6361 gcattttgcg aaaacctcac tagaatatta cgaacactag tggtatacta agttcaattt
     6421 caaagataaa tggaattcaa aaaggtggca gatcgataat tatgacagaa aaatggcgtg
     6481 aagacgaaga atatatgtct tatataaatg atctattaga aactgatgca gtaaagaaat
     6541 tagcaaatta cacgcaacat gtccactcga cacgattaga gcattcaatc agtgtgtctt
     6601 actatagcta caaattagcg aaaaaatggg gcggtgatgc aagagcgact gctcgagcag
     6661 gccttttaca tgatttattt tactacgatt ggcgtacgac gaagttcgat gaaggtacac
     6721 atgcgtatgt tcacccaagg atcgctgtga aaaatgctga aaaaatcacg gagctttcag
     6781 atctagagcg tgatattatt ttgaaacata tgtggggagc aacgattgcc cctcctaaat
     6841 ataaagaagg ttatatcgtt acttttgtgg ataaatattg tgcagtcaaa gaagcagctc
     6901 aaccaatgac agcttcaatg aaacaaaaat ggcaacgata tttcggtaaa gaacaatcga
     6961 tttaaggtag ttaactaagt atttgacccg agtattttga aaaaaatcct cgggtttttt
     7021 gtttagacat tgactaattg gtctttttag tgtttacttt gttgagatga aaaagtacat
     7081 aaccaacata aaaataaagg gggtgatgat aagatgcgat gtgtatctta tttaaaagag
     7141 tttgagggct ttatctaaag gcactttgat agaaagatca cttttttctt caaagtagcc
     7201 gattttgaaa gaagaaagga ctcaaagtca tggaaaaaac aatcaaacca tatcaatatt
     7261 attttatcaa gcgagaagtc gagcagctgt taagcgccta tagttcagtg aatgatccaa
     7321 agacagtcca aacagtccaa gcattagctg cggaaaagat ccacgaggta cttggtcatc
     7381 ctaatacagc tgtaaatgaa tttatggaaa aggtactaga cgtcactcta aaaaaagaaa
     7441 aagcagaacg actattagag gaattgaaag tgatcgttct tccattcata caaccgacaa
     7501 aaccgcagat cgataaatta ttccgaaaag tgaaaaagtt aaaacaacca gattggaatc
     7561 aactggattt aagggaacat acttatattg gttggaatga tccaggtagc caaaaaaaat
     7621 ttatgatcta ttatgaagaa gaaaaactaa aagggatcag tggaacatta tctcctacaa
     7681 tcaacaaagg aatttgttcg atttgtcaaa gaacttcaaa tgtttcactc tttctttcta
     7741 ccacaaaaac tggttcagat ggaacataca cgaaaaaagg aaattatatt tgtcatgata
     7801 gtgaccaatg taatcagcag ttggtacaat tagctccttt ttacgaattc acagaacgag
     7861 tgaaacgtga gcggtgattt cattgtattt ttctgaaaaa tatgctatct tatttggtga
     7921 agtgaatagg tgatggtgtt cgcctttaaa cgttgatcgt aagataacta atgacgccta
     7981 ctaaagaaac ggggctaccc ctttttttag taggcgtttt tttgaggagg aaatgagatg
     8041 gaaaatcgat tacctgatcc attgaaggtg atcatctata tgatcaaatg gttattgatc
     8101 acagcaattt taggtatttt tatgggaagt ttatccgcat tctttctaac tagtttgact
     8161 gatgtgacag ctattcggct ggagcatcct tggttacttt ttcttttgcc gataagtggg
     8221 ggactttttg cttatctata tacgacgtat gggggcaatt cttcccgggg aaacaattta
     8281 gtgatcgatc aagggaatgg aggaaatgaa aagattccat tacggctgat cccactgacc
     8341 ttatttggca ccattaccac gcatcttttt ggtggttctg ttggtcggga gggtacagcg
     8401 gtccaaatgg gcggggcact tgctgagaat tttgctcgtt ggctaaaatt gaacaaaatc
     8461 gagcgtgaga ttcttgtcat cagtgggatc agcgcaggtt ttagctcagt atttgggaca
     8521 cccttggctg ggacattgtt tggattagaa gtattggcaa tcggaaaagt gagaaccgaa
     8581 gcactgtttc caagcttttt tgcagcactt tttgcgaact ttgtaacgga aagtttcaat
     8641 gtcaagcata cccattatca aatgggggcg attcctgcat gggacccttc acttttcttc
     8701 aaattgttgc ttgcgggcat tgcatttggt atcattggct ggctctttag ccgttcgatc
     8761 gtctggttaa aagcttggta tgcaaagcgg atcccccatc cagttttacg caactttatt
     8821 ggaggagcag tcgtggtact cgtagtagtg attttacaga cgcaaagata tctcggatta
     8881 agcttacctt tattacaaga tgcatttaat ggaagtgctc atccttatga ttttcttggg
     8941 aaacttggct ttaccgttct atcacttggc gcgggatacc aaggaggcga agtgacccct
     9001 ttgtttgaga ttggggcaac tttaggtgcg acattagcac ccattcttca tgtatcagta
     9061 ccttttttag caggattagg gtttattggg gtgttctctg gagcaacgaa tacaccgatt
     9121 gcttgctttg ttatggggat cgaacttttt ggaggtcaag cagccagcta cttctttatg
     9181 atctgtttga ttagctttat gtgttcagga aatagtggaa tctattctgc gcaaaaagtt
     9241 gaagtgaaaa aaggaatctt gttcctaccg atccttggaa gaatctcacg acaaaaagag
     9301 aaataaaaag aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa
     9361 aattgctttt taaatttttg tgaatttcgc ttatttccga aggagttgct tctgctccca
     9421 ccgtttattc ggagttagag tctgtggcaa aactgatttt tagttttgtc ccacgctctt
     9481 ttttattgag caaatttgag aataagagca gaaaatactg caaagccaat cattaagcca
     9541 cagatgataa gatggcgttt tttgtgtaag acaaggatgg caataaattc tcttaagata
     9601 gcattcggta agtagtatag tgcagttttt gcaccgattc cgtttgcatt gagtttcgct
     9661 tgtcgcgcat atagtccagc tctaaagata tggtaattat ttgaagtaaa aatcgcgcga
     9721 taaggcccat tcatttgctg atccattatt tcttttgaaa acagcatatt ttctagcgtc
     9781 gttgtggaat tcgtttctac taaaatatca cgctcgggaa tccctttttc taaagcatat
     9841 tgtttcattg caatggcttc agctactttt tcatcggttc cttttcctcc tgacatgagc
     9901 aagactggcg gattcaaagt tgctttactt tgtgcccaat aaaatgcgat cgccttattg
     9961 atccttttag caagtagggg agatacgcgt tcaccatcaa tcaagccagc gccgagaaca
    10021 acgatataat cttggttata ccttggacgg ttgaactgat acaaaattga aacagtcata
    10081 aaattaaaga aaacgatgag tagataagca aaaataaacg gaatgacagc aaatagagca
    10141 tttgcccatt gtgggagcca atggactaat gtatagtcca atatcaaaaa gatagtcaaa
    10201 gcgactccta acaagagagt caaaaggttg gccaaagaac gggattcttt tttcatgact
    10261 acaacaccat tccaaaataa aaaaatgatc aaaatgtaga taccgattgc caaaagaatt
    10321 aatattggta tcccgatcaa agccaataac ccaccgataa agatattttg cgtctctatt
    10381 agtagccacc ctagataacc accaaagaca atcaaaaacg tattaaaaag tacgccattg
    10441 attaacctac gtttttcctt aaaatagaaa aatgcaaaaa tactgaaaaa tagcaatgga
    10501 atcaaaaaat aaaatgtatg ataaaaaact tctaaaacga cgaagtaagt gaccatcatc
    10561 ataaaagtcc acgtcccaca ataaaagaat agagcgggat cgctttggcg cttgcgccaa
    10621 tagaggatgc ctgacattcc taaaatatag ataactaaaa atatcaggtc tctagtaaga
    10681 ttcattgaat gaactccttt cgatttgtaa attttttctt aaaaaagata gcatcattta
    10741 gtagtaaata tatcgaatat agaaaagtta gttactaaat agtcaatcag ttttttcacg
    10801 acgtttctat tataacgaat tatgttgaaa aaagctttta tcgaaaacat aaatcttcaa
    10861 attaacagat tttttagacc tcgatcatga tgaagacttt tgtagataca aataactgta
    10921 acattgaaaa tgaatgcgaa aacgatattt ttttttattg gaaaatatcg gtcaagcaat
    10981 aaaccagttg cataataaaa tgttaacggc aacaaaatct gtggaaggaa tgaaccggat
    11041 gatttgatgg aaaaaacaag atagacaaga agtatacata gtaaaacgtg gaacaagtag
    11101 gccagaatga aaaatcagtt tttatccact cgcttttcta tacattcact gtcacaaata
    11161 taatcgaaag taattctatt ttgagataaa gtattttata ggagatagta tatgaacaaa
    11221 aaaagattta aacgtattga aagaaaattt gcattaactt atgaccaagc taagtatttg
    11281 aaaacactat ttccaaaaca ggtagttcgg gataaatata gtgatcattt tatttcttcc
    11341 ttgtattttg atacaaacga tcatcacttt ttaaaagaaa tcactacaga aaagtcctat
    11401 cgagaaaaat ttagaatcag aaaatatgga gatcctcagg acatatcgcc tatctactta
    11461 gagataaaaa gaaagtttca aggaatgaca gataaacgta gaataaaatt agcgcatcat
    11521 gagctcgatg atatgttgga aaatatcgaa ttggcagaaa ccacattcag taatttagaa
    11581 gaagagtata tttttaatga aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa
    11641 gtatatatct attatttaag agaagcattt gtaaatagag attcagaaga tttgcgaatc
    11701 acttttgatc aaaatatcca ttaccgtcta atgaacttaa cgacctggga acactgtaca
    11761 gaagacaatc tggtagcgcc agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa
    11821 atacctgaat ggttacaggt aggattggat aggatgggta aaaaagagat aaaattttct
    11881 aaatataaac aagtctataa aagatatatc caagaggacc cgctctatgt tcaatagtgt
    11941 cttaaaaaat gagccttctc aactcacact agatgatttc tttttatgta tggtggtgtc
    12001 gattttgcta ggcattctga ttgcttacac ccatatgtat caaaacaact gtacaaaaaa
    12061 cttcatgttt gccttgattg ggttacctac tttagtacat accgtcattt tgttgatcaa
    12121 cggtaactta ggcacgggga tcgctgttct aggcgcattt aacttagtgc gatttcggtc
    12181 tgctcaaggg aattcaagag atattttcag tattttttgg gcaacgtcga ttggtttagc
    12241 cacagggacg ggatatattg tctatgcact attcgtgtct agtatagttg ctgctatcca
    12301 agtcatcatc ttcacgcttc caatcaaaaa taaacataca gtttttaata ctgaaaaaac
    12361 gatcaagcta aaagtttcta aatccttgtt tctttcgagt attattgaag aaaaaattgc
    12421 agaatataca gaaaactgca atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt
    12481 acactatata gtaaaagtaa aaacgagtat gcttgaaaaa gaattggttc gaacgatcat
    12541 ggatcatcca gaagtcattt cgatcactgt aggtatggat aaatcgaata aattattgtg
    12601 ataaccaaga aagtacttaa caatgaagta agaccccagt tccaaagttt agcggggaca
    12661 cttcaataaa gtactttctt ttttgtcttc agcaactact ggtaaaactg agagcaagct
    12721 ttcgtgagag tacagcgata aagatcaacc tcccttttta tctcgagcaa tttccttgca
    12781 aggctgtttc gttgatctta aacgagttag caacagtcaa agatgcgatg gccataaaaa
    12841 aattgcatta agttggctgg tatctgatag ctgggaatct aaggaagact atccattctg
    12901 tttcttaaat tccgctagtt gatagatatc agccaattgt tccagatgat caacatcaaa
    12961 gataaccgaa agatgtttcc ttttattccc atttgcttga agagttttta tcttctcttt
    13021 ggttccttca ctcaatgaga gtgtgactaa tttctccctc gttttttctg ggaaatgaaa
    13081 ggatactttg aaaaatccgt cccaaataga aagccagaag atcggtttta ctttctgaga
    13141 gtgggacaaa agtaagaaac acttttgtct cattctttaa aaacgaataa acggtgggag
    13201 cagaagcaac tccttcggaa ataagccaaa attcacaaaa atttgaaaag caattttcgt
    13261 gaatttcttc ttatttctcg gagttaaaca cttctgtccc aacctcttgt attcaccttt
    13321 tgatagccaa gcttttccat cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac
    13381 aaaaagcgta ttgctcgttt ttctcttcag tttctcacac ctaaacgact tttgtctcga
    13441 cctctttttg gtgactattc atgatgttaa aataaattac tgaaaaaatc tgtgacatgg
    13501 cgccaaccaa tgacaaaaat attagctttt tcaacagtag aagtggtaat gatgtctgtc
    13561 gaaggctctt ggtttgcttc caaatagcct aatttgtcat cagctaattg gatcgttgct
    13621 gagccaactg tagtgccttt gttgatagga gcggtcactt ggtcatccgt tactttcttt
    13681 ttatcaattg ttggagtaat cgttaatttt ccagtatcca ttccattttc gacccagact
    13741 tttacagggc tttttagcgc aacattgacg gaggtttctt ttccatcttt cactttgatg
    13801 tcttttaggc ttgggatgct tgcatttgct tttccaagtt ctttttctga ccagttgtca
    13861 taacaataat ccatcaatcg tgatgtttca ttgaatcggg cacttggatt ttcttcatgg
    13921 ttatctgcat ttaaaacgac tgtgatgatt cgtcggccat ctttgttgat cgttccgaca
    13981 aagcatgcac ccgcaaaatc agttgtgcca gtttttaatc catcgacacc ttcgtgatag
    14041 tttacaaaac caggtagcat ccagttccaa ttgaccattt caaccggtga ctgtgtgttt
    14101 tcgccgaatg tttgatttgt ggtactcgtg atttttaaga cttcaggata atctaagatg
    14161 aggtgacgag caacaatagc gacatctttc gcagacagtt gattttcatc tgtttcacca
    14221 gtattctcag gtcgattctc acctaggtaa gaattgttta aacctgatgc attgacgatc
    14281 gttgcatctt tgatacccca atcttttagt tgtttcttca ttagctcaag aaatttactt
    14341 tcactgcctg caactttttc agctaaagca accatggaag cattggctga ttggatgaaa
    14401 gctgagtcaa caagttcttt cactgtataa tcattttctt tatgcaaagg aacattcgat
    14461 agatccggca cgacacttaa tttttctgca taatccgaaa tggctacctt atcttcccaa
    14521 gataatttac cctctttgat ttgttcaagt acgatatata gaccaatgat ctttgtgatg
    14581 gaagcaattc ccatcgtttt ttcgccatcc tgatcataaa ggatttttcc actttctgca
    14641 tctacggcaa aggcagcttt ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg
    14701 ctaggcaaga aagaagtgat acataagata agacttgcta atagtacaat aaaacgggta
    14761 ttttttttga acatctttgt tctcctcatt tttaaattga ttcttacaac ttagaatatt
    14821 atcagagaga acaaaaggtt tcaatggttc tgaaaaaaac cttataaaga acaagtctta
    14881 ttaatgaaga atcatagaaa gttatctttt gggttcattg acacgaactt tgactggctg
    14941 aacttcgggt tcggttaggc gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa
    15001 actaaaaatc atgaataatt gcataagatc acctcgttct tttttgtttt tttatagtat
    15061 agcaagagag tttgaagcta ctgttaagga aatgagcata taattgatga aagtatgaaa
    15121 aaaatatgaa gtaggacaac acaactaaaa atcgatcagg tatttctctg gtagggattc
    15181 gttttattgt attgataaaa tgggatgtgg tagactttat ttacagatac aaagaaatga
    15241 tttttttagg aggggtacgt ttatggcaaa aaaagaaaaa gaattattat cacaattgac
    15301 ttcagaaggt accaaaggac cgtttgtcac gattatgtta aacacccatg ttgcgcatca
    15361 agacgtagaa aaagatcaaa tcaaattcaa gaattttgca aaggaagcga aaaagcgctt
    15421 tgaaaagaaa tactctgagc atgattggtc tccattccaa gaaaaaatta ctagtttgtt
    15481 ggacgatccg tctttctggc gtagtgccac aacgagtgta gctgtcatag tatcaccaga
    15541 acaagtgttt atccaccgtt tgggtattcc tgtagatgac cagtattatg tgagtgatct
    15601 accttattta ttagcgatca tcaaaaatgg acatttcaac tattcatact atttattagg
    15661 attgaatcgt gattcgatga aattgtacga agtaacgaat aaacaagtca aagaagtggc
    15721 tttgcctgaa gatgcaccaa gagatgtcgt cactgcttta ggatctgaac taacaggagg
    15781 cgggttgaac tacgcatcaa atggtagtga aggtacgacg ttccatggag tcaatcctaa
    15841 agatgaagaa gtcgaaattg attgggtcaa ttattatcaa gcagtcgatg actatttgaa
    15901 agatacattt gaaaatccag agaaatggcc tatcttctta tttgcattac cagaaaatca
    15961 gacgatgttc aaaaaagtgg cgaagaaccc ttattatcgt tccgatgttg cgatctcagc
    16021 ttcacctgca caactttcat taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga
    16081 attagctgtc aaagaagcaa gtgactacaa taagttgctt gataaaaagt tcattgatca
    16141 actcgttgat attataccgg cagcaaaaga agggaaaata tcccatttat tcattgctac
    16201 atctaattta gcagatggtt ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt
    16261 gctgaatgaa ctagcagata atgtgatcac gaacggtggt caggtcttta tccttgaaca
    16321 aaaagatgca ccagacgaaa agagtttagt tgcgatctta cgttattgag tgaaaactac
    16381 caaagtagat gaatggtcag gcgttgatag aagtgtatag ctccgagaga aggagtttcc
    16441 tctgtttcag ccgttggatt catcttgagc gagagacaaa agctagcttt tgtctctcgc
    16501 tcattatttt tgtgctttag tcccattggc tttgcgaggg gaagatactt ttctcaaaaa
    16561 aacgaacctt gcagttgaga gggggataga tgcgcatact aatttttatc agttaaaaaa
    16621 tgatgtggat cattagagaa ttccaaagtt cgttcatcct ttttcagata ataaattgaa
    16681 attttttgca aaagtgaaaa tagcaaatag aaattttctg aatcttatag gggtgatttc
    16741 gagaaaacgt tcgtttattt gcaaatatat cttctaaata agagaataaa tacaaaaaaa
    16801 taaggaaaaa ctctggcaat tttgtaaacg gtatgatata gtaagaatgt agtttttgtt
    16861 cggtaatctg tggtagcttg tttcaaataa acatcttcca gatatacacc attcaagtaa
    16921 ttgcaaaata tgtgttgata cacaaggagg atttttatta tggaaacagg tacagtaaaa
    16981 tggtttaact cagaaaaagg atttggattc atcactgcag aaaacggaaa cgatgtattc
    17041 gttcatttct cagctatcca aggcgacgga ttcaaaactt tagaagaagg ccaagcagta
    17101 actttcgacg ttgaagaagg tcaacgcgga cctcaagcaa caaacgttac taaagcttaa
    17161 tttacctata ttaaaaataa aagtccagac atttatgtgt ctgggctttt ttgtcttttt
    17221 ttccttaaaa agcgatgaga gtatttttct gcactttgta gtataatttc aaaagaacat
    17281 tagaggcagg agaagaggaa gatattttga aaccaagaac ttatgcagtc gttgatatcg
    17341 agacgactgg gacgaatcca aaagaagatc ggatcatcca atttggctgc gtcatgattg
    17401 aagctggtcg gatcacttca cgtttttcga tcgatattca tccagggcgg aagatttcaa
    17461 aacaaatcca gcatttaaca gggatcacga atcaacgcgt ccaaaaagcg ccttattttg
    17521 aagatgtcgc ccaaacgatt tacaatcttt tggcagacac catatttgtc gcgcataata
    17581 tttattttga ttataatttt ttgaaccatg aactcatgcg atgcggactt cctagtttga
    17641 agataccggg catcgatacc gtagaacttg cacaaatttt cttgccgacg gaaccaagtt
    17701 ttcgcttagc tgatttgtca gaaagccttg gctttattca tgagaatcct catcaggcgg
    17761 atagtgatgc cgaagtgaca ggtcagcttc ttttactgat cgaagaacga atgaggaaat
    17821 tgccgatcat cacattagag cagattgcac gattaagtag ccatacagga atggatacga
    17881 gtcgtttcat ttaccacgtg cttgaagaaa tgaaagaaaa cccggagccc cttgatctct
    17941 cattagaaat catcgatggg ctagctttgc agaaaaaaga agtggagcta ttcacatccg
    18001 tccattatgg cgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta
    18061 aaacactgat gtatcgaaaa gaacagaaca ggctgatgaa tgctgtctac gaccatttca
    18121 caaaagatga atcaaaagat ttgatgatcg aagcagctac aggaatgggc aaaacgattg
    18181 gttatctgtt gcctttgagt tatttggcta cacctgaaaa accagctgtc atcagcaccg
    18241 tttcgcttgt tttgcagcag cagatcatgg aaaaagacat tccattactg aatcaattgc
    18301 tagatcagcc gatacaacct gtgattatca aaagctatcg ccactatatt gatttacagc
    18361 gttttaaagg tacacttgtg gaaccgccgg gacaaaaaca atatgctttg taccaaatgg
    18421 ctgtcttagt ttggctaaca caaacgaaga ccggtgattt ggatgaatta catttaacca
    18481 atctgaacca tgcattcttt gcagatgtcg cgcacagagg cactgggttt ttagcaagga
    18541 atcagtcatt ttacaaacaa gactttgtgc gtttcttgca aaagaaaatc aaacaaagca
    18601 attttttgat agtcaaccat gcatttttag tacaagaaac acagcgaaaa gaaccattgc
    18661 ttccagctag tccttatttg ttgattgatg aagtgcatca tttgccggaa attgctgaaa
    18721 aagtaaatga caaacgacta agtattaact acttccataa acaaattcag caactggaag
    18781 agccgaatca gttatttgac cgcatacaaa gttatttgcc gaaggaacat gaagccttgc
    18841 gctggttgtc gatttatcga gaagaactct ctgcactatg cgaagcgcag atttgcgtgg
    18901 cagagggatt aatgaactta gtaagcagca agcaacctaa cggacaatat ccagaagaag
    18961 tcatgatgac aaaagaaatg ttggatcagc tgccactgga atctggaaca gcaatggatc
    19021 atctgcaact tttttatcaa gagatccaac aactacaatc acagatgcgc ttgacttttg
    19081 agattatcca agaaagttgg accaaccaga tgcggatcga tttagcaagt ttatatagct
    19141 tatttgatca attagacaag caatacttgc tgatggacca atggctagaa gactggcgag
    19201 atcatttgat ccattggttc gttcccggga atacagcaca gcagatgatt tttcatctcc
    19261 ttgattttca agcagctatg ttgccgtcaa ctgtgtggta tcctcgatat gaccatattc
    19321 tttatattgg aggaacactg aagatcggac ctaatcgaca gtatctctct aaacgacttg
    19381 gcattgaagc agcacctctt aaagtgattg cttctcctta tgattatgag aaacaggcaa
    19441 gaatctatgt gccgaaagaa gcacctgcag tccatcaagt cagcaatcaa gcatatatcg
    19501 attatttaga agcagcactc acaggattga ttgaacaaga agatcggccg atccttgttt
    19561 tattcacttc tcatgatgtt ttgcaaaagg tatatcagaa aatgcatttg tctatgctag
    19621 aaaaaggacg tgaaattctt gctcaaggat ttggcgggag tcgggaaaaa cttttgaaac
    19681 gcttcatttt gtcagaaaaa agcgtcttat ttggtgctga cagcttttgg gaaggcgtag
    19741 atttacctgg agatgctttg cagattgttg tagtcacacg tttacccttc gacaacccgt
    19801 tacgtccgat ggtcaaagct aggaatgctt atctggaatc agaggggatc aatccttttt
    19861 atcaggaatc cgtgcctaaa gcagctttga agttacgcca agcattaggt cgtttgataa
    19921 ggacggagac agatcgtggc gtattactta ttttggatcg gagattagtc actgcaaaat
    19981 atggtgcacg aatcatccat gcattaccga aaaaagtacc gataaaagag ctagctatag
    20041 atgaaatcta tcaggatatc catgaattct taaagaaaaa tgaagaagga agttaaaggt
    20101 gccgtctttt ttcatcatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga
    20161 cgacccttga agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt gcttggcttg
    20221 atcgtatttt tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt
    20281 ccggcggtcc aggcgaaaaa agaagcagta gaaatcgcaa agaaatatgc aaatctcgag
    20341 gaagccgatc atttttactg gtttactcga aagaaaacgt acttttcatt gacgggtacg
    20401 aatgataaag gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta
    20461 ttggatcaaa caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat
    20521 cctgaaatcc aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa
    20581 gtagtaggaa aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga
    20641 gatgaagtca agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat
    20701 caaaaagaac aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg
    20761 cattaaaagc aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga
    20821 caccagaaaa catccaagaa gcagcaatcg aagcaatccg caatgggaaa gctagctatt
    20881 atacaccaac agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa
    20941 actatggact tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt
    21001 tgtataccct tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt
    21061 actgggtcag ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag
    21121 gggaagaatc caatagctgg aaagttactg ttgagcagct agaacaagca cgttcagaaa
    21181 aaacaaaagc cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga
    21241 atgaactgca ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg
    21301 atatttatgg ccgtttagtt tataatggca atgagtttac tccaattgca acgatttcag
    21361 aagcaatcaa aaatcagacc atcatcatca atggcgtgtc taaaacatat gcgatgacag
    21421 gctggcgcat tggttatgcg gtggggaatt ctgagatcat caatggaatg attgctgttg
    21481 cttcccagtc aaccagtaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga
    21541 aaggcgagca agatacagtg gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt
    21601 tatatccgct tgtggcagaa ctgccgggtg tttctcttga aaagccacaa ggagcttttt
    21661 atctttttcc taatgtaaaa gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat
    21721 gggtagaaga tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg
    21781 caccagaaaa tgtccgcatg agttatgcca ctgatcgcgc tacgttagaa gaagcggtac
    21841 gcagaatcaa gcaatttatt gagagtaaaa gtcaaaatta atcctgaaga aattcaagga
    21901 atataaaagg agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg
    21961 gagaaacagt aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat
    22021 ttttgcaatt aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgc
    22081 cggaagaagt tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg
    22141 gagaaatccg tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg
    22201 aagtggtaag cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct
    22261 tgatggatca tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca
    22321 gaaatgaaat cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaatcg
    22381 atccgccgat cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt
    22441 actttgatca aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta
    22501 tggctttcgg aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac
    22561 gacgtcattt gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag
    22621 aaagtttaga agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt
    22681 gcgaatacgc attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat
    22741 tgccattccc tcgtattact tatgatgatg cgatcgaatt actgaagaaa aatggatttg
    22801 atgacatcga atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat
    22861 tcgaacagcc tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac
    22921 cacatccaac aagagaagac gtggtgatct gtgcagatat gatcgcacca gaaggttacg
    22981 gtgaaattat tggtggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc
    23041 gtaatcacgg tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt
    23101 ctgttccgca ctctggtttt ggtctaggcc ttgaacgaac agtcacttgg attgctggta
    23161 tcgaccatgt gcgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag
    23221 aggttgtgaa atcagtgcct tgacctgagt attagagaaa caaaacggaa aaatagcttc
    23281 tcatatttta ctgttttttg agctaatatc gagggtcagc tgattgaaca ccgtttatat
    23341 caagaggttg tgcgtctgcc gttcagctcc gagtcacaag gagtaatttc aaaaaaacat
    23401 tttttcatgt ttgatgacta ttgtgacttg tgaccaagga gttggccgga gagcaacgtt
    23461 tatttaaaaa aaagaactgc gaactctcgc agttcttttt ttttaaatac gggataaagg
    23521 aagtttccag cagataagag aagaaagcaa aagaaattgc tatttgattc acaaggttct
    23581 ataacgaaat cgcaatcaat caaacatata caaagaaggt tattcaaatg gaaatctgat
    23641 ttgatgatgc ttctgctgtt gaaataaagc agcatctagc acctgaaaac aaactaatag
    23701 taacattcga agatggagta ggtccgtatt ttcagcatgc aatgatccat atgtaagttc
    23761 aattcaccct tgaaatcatc ggaccagata aagttatggc caatctatcc caagcaatct
    23821 agaagagata ttagtaaagg aacatttact ggaagtctta gatgagccta taaatatatg
    23881 ttctgatggc gtcatcaagt ggtctgattg acggaagctt aggtgtgatc gatctctctg
    23941 gaccaaacaa tgaaggaatc aaggggaatc cagcacgtta agaaaaaaat agcataaaaa
    24001 actagacact tgatgagcgt tcattcaatt gtttgaaggg actaggctgg tgtcattttt
    24061 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
    24121 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
    24181 acagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
    24241 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatggaattt gaatgcatga
    24301 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcga gaagattgtt
    24361 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
    24421 ctagcgatca ggctatattc ggaactgaga aatctgctca gcaatttgaa tccgtttatc
    24481 gaaaattact aaatgaactt caaaaagcgg gaatctgcaa catcatcttg atggagccgt
    24541 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
    24601 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat
    24661 tcaatgcttt agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc
    24721 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
    24781 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
    24841 ggcagtaaaa gaagatctac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
    24901 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
    24961 aacaaaagaa aaacggatta tcggttttgc gcaagcggac gtgcaacagt cagaagaacg
    25021 ggtgggtcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
    25081 ggaagaaatc caaaaaaaat ttgcagaata tttgatgctg gaagctaaaa aaagaagaat
    25141 aaaatcaatt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
    25201 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
    25261 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
    25321 aagatcgcct tcctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
    25381 atgtcattga tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
    25441 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
    25501 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaagaga
    25561 ataataaagg ttagataaag catagagtca ggtgggatac atgtatttca cctgactcta
    25621 tgcgttttcc tgtataagtt cttccatgtg acttttacgt cttttattta tatggggtta
    25681 ctgtttatgg aaagttgaaa taattttcat taaagaactc tttctatatt tttgaaagcg
    25741 tagtggagct tcgtatatct gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc
    25801 tatcgaaaaa ttaatgaatt tagacaaatt gccagcaaca ggcttcaaac ttagttgcta
    25861 tcctgtcaag atcaagaagg caagtgcggg ttggataaga gcagtggcaa tgatcgaaga
    25921 gaaaaagaaa gaataagcca aaacatctgt atggaagaaa ttttccgatg cagatgtttt
    25981 tcattttttc tttcggatac cttgacatat gaataaccat tcatttataa ttagttatga
    26041 agagatattc atatgttgag gcgaggaggt cattaaaatg aaaagttaca gattagaagg
    26101 tttagattgt gcgaactgtg caatgaaaat cgaaaaagga gttcagaaaa tcaatggagt
    26161 caaggaagca acggtaaact tcacttctgg aaaactaacg attgacgcgg aggaagatca
    26221 tttggctacg attgaacaag aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa
    26281 agtaacagaa atagataagg aaaaagtatc tgaacatgga aatgaaaaag agagaaatac
    26341 gctgttccgg attttatttt cccttgcagg tatcgcttta ttgctattat ttgattttaa
    26401 tgaactaatt cgcttgatag ggtatttatt gatttatctg ctgataggat atgatgtagt
    26461 gaagaaggca gtgatgaata tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc
    26521 tgttgctacg attggcgcta tgttgatcgg tgaatatcca gaagccgtag cagtgatgct
    26581 attttatcaa atcggcgagt atttccaagg attggcagtc agtcattcaa gaaaatcgat
    26641 tcgcgaattg atggcgatcc gtccagaaac agctcacgtg cagacggcgg aaggattgat
    26701 gaccgtgaac cctgaagacg tgctgatcgg tcagtttgta cttgtgaagc ctggtgaacg
    26761 tgtaccgtta gatggaacga tcatcgaagg agaatcatta gtcgatacgt cagcacttac
    26821 aggagaatca gtccctaaat ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa
    26881 taaaaataaa ccatttttag ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa
    26941 attattagaa ttagtagaaa atgccagcag taaaaaagca ccagctgaaa atttcattac
    27001 taaattcgcg cgctattaca caccagtcgt tgtaggattg gccgttttat tagctgtttt
    27061 acctccactt gttgtttctg gagcggcgtt ttctgaatgg atctatcgag cattgacatt
    27121 tttagtgatc tcatgtccat gtgcattagt gatttccgtg ccacttagtt tctttggcgg
    27181 tatcggagga gcaagtaaaa tcggtgtatt agtaaaaggt tcaaattact tggagttgct
    27241 tgcgcaaacc gagacagtgg tattcgataa aaccggcaca ttaacgaaag gcgacttttc
    27301 gatccagacg atcgatacaa aaatcgatcc aaagatattc atgcaatata ttgcgagtgc
    27361 agaacagttt tctacacatc cgattgctca atctgtttta gaaggctacg caggtccgct
    27421 tttaccaaca gctaatatcc aggaatttgc aggtgaaggg atcttagctg aagttgatgg
    27481 caaacaagta ttagtaggta atcacaaatt gatggaaaga tttgaaatct cttttccttc
    27541 aagtcaagaa atcggtacat tgctttatct agcaatcgat caaagttaca gtggttatct
    27601 tgttattgca gataccttga aagaagatgc cgtagacgca ttggttcaat taaaacaggc
    27661 tggtgtgaaa aacacagtca tgttgacggg cgactcgaaa aaaattgctg atcatatcgg
    27721 gaagcaagta ggcgtggaca agatatatag cgaattgctt ccagaagata aagtgcagcg
    27781 gttagaggaa atcttacaaa gtaacaataa aaagaccgca tttgtcggag atggaatcaa
    27841 cgatgcaccg gtgctagcaa gagcagatgt aggaatcgca atgggtggat tgggaagcga
    27901 tgctgcaatt gaagcagcag atgtagtcat catgaatgat caaccatcga agattgcaga
    27961 agcaatccat ttagcaaaga aaacattgaa aatcgtcaaa caaaacattg tctttgctat
    28021 tgggataaaa attttggtac tttccttagg tgcttttggt tttgcatcaa tgggagatgc
    28081 agtatttgca gatgttgggg tgacagtatt agctgttttg aatgccatgc gaagcttgca
    28141 tgtaaaaaat aaacgttgat caatgaagag gacgaatgaa cgtcctcttt tttttatatc
    28201 cttttcttaa tgaaaaatgt aaagaaaata gataggatta taaaataact
//
LOCUS       DAXWLB010000017        27840 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXWLB010000017 DAXWLB010000000
VERSION     DAXWLB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.017"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(399..1343)
                     /locus_tag="ACGE4H_000584"
     CDS             complement(399..1343)
                     /locus_tag="ACGE4H_000584"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8364024.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1639..2583)
                     /locus_tag="ACGE4H_000585"
     CDS             complement(1639..2583)
                     /locus_tag="ACGE4H_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8364025.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2804..4018
                     /locus_tag="ACGE4H_000586"
     CDS             2804..4018
                     /locus_tag="ACGE4H_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8364026.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4040..6055
                     /locus_tag="ACGE4H_000587"
     CDS             4040..6055
                     /locus_tag="ACGE4H_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFF8364027.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6138..7511)
                     /locus_tag="ACGE4H_000588"
     CDS             complement(6138..7511)
                     /locus_tag="ACGE4H_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8364028.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7537..7821)
                     /locus_tag="ACGE4H_000589"
     CDS             complement(7537..7821)
                     /locus_tag="ACGE4H_000589"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8364029.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7823..9838)
                     /locus_tag="ACGE4H_000590"
     CDS             complement(7823..9838)
                     /locus_tag="ACGE4H_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8364030.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELWSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10017..10322)
                     /locus_tag="ACGE4H_000591"
     CDS             complement(10017..10322)
                     /locus_tag="ACGE4H_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFF8364031.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10411..11442)
                     /locus_tag="ACGE4H_000592"
     CDS             complement(10411..11442)
                     /locus_tag="ACGE4H_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8364032.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11649..12524
                     /locus_tag="ACGE4H_000593"
     CDS             11649..12524
                     /locus_tag="ACGE4H_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8364033.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12676..13518)
                     /locus_tag="ACGE4H_000594"
     CDS             complement(12676..13518)
                     /locus_tag="ACGE4H_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8364034.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13610..15487)
                     /locus_tag="ACGE4H_000595"
     CDS             complement(13610..15487)
                     /locus_tag="ACGE4H_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8364035.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15484..16905)
                     /locus_tag="ACGE4H_000596"
     CDS             complement(15484..16905)
                     /locus_tag="ACGE4H_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8364036.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16908..20297)
                     /locus_tag="ACGE4H_000597"
     CDS             complement(16908..20297)
                     /locus_tag="ACGE4H_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8364037.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20554..21945
                     /locus_tag="ACGE4H_000598"
     CDS             20554..21945
                     /locus_tag="ACGE4H_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364038.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22018..22665)
                     /locus_tag="ACGE4H_000599"
     CDS             complement(22018..22665)
                     /locus_tag="ACGE4H_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8364039.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22673..23359)
                     /locus_tag="ACGE4H_000600"
     CDS             complement(22673..23359)
                     /locus_tag="ACGE4H_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364040.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23365..24438)
                     /locus_tag="ACGE4H_000601"
     CDS             complement(23365..24438)
                     /locus_tag="ACGE4H_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364041.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24660..25334
                     /locus_tag="ACGE4H_000602"
     CDS             24660..25334
                     /locus_tag="ACGE4H_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8364042.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25339..26679
                     /locus_tag="ACGE4H_000603"
     CDS             25339..26679
                     /locus_tag="ACGE4H_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8364043.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26828..27814)
                     /locus_tag="ACGE4H_000604"
     CDS             complement(26828..27814)
                     /locus_tag="ACGE4H_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFF8364044.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8120 a         5262 c         5040 g         9418 t
ORIGIN      
        1 gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa
       61 attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt
      121 attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag
      181 gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat
      241 taaatttcat atgttcatat gtacggatac attttcggac cgattaattt taaccattct
      301 gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt
      361 aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt gttcaatact
      421 tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat
      481 tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct
      541 ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc
      601 aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta
      661 cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc taattcatct
      721 tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta
      781 tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg
      841 cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt
      901 gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc
      961 aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt
     1021 tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga
     1081 ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca
     1141 tagccacctg accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc
     1201 aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt
     1261 ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact
     1321 actttacgac ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt
     1381 catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat
     1441 tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta
     1501 cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat
     1561 tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt
     1621 tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata
     1681 cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc
     1741 ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag
     1801 aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg
     1861 attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat
     1921 ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg
     1981 ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg
     2041 gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa
     2101 acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga
     2161 tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc
     2221 tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta
     2281 ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat
     2341 cctttctcca ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa
     2401 atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc
     2461 ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt
     2521 taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg
     2581 caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc
     2641 tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa
     2701 agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact
     2761 tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa
     2821 gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac
     2881 gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt
     2941 attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc
     3001 taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttatgg
     3061 tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg
     3121 tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg
     3181 agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt
     3241 aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta
     3301 cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac
     3361 tgaacttcaa tcacatggca cagccccaac aagacaagta atcaaaacga atcctaaaat
     3421 gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa
     3481 attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt
     3541 aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa
     3601 agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat
     3661 tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt
     3721 ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga
     3781 gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata
     3841 tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa
     3901 tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa
     3961 tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat
     4021 ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc
     4081 tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt
     4141 tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata
     4201 ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga
     4261 aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa
     4321 aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga
     4381 agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg
     4441 gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg
     4501 cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc
     4561 agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg
     4621 ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg
     4681 aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt
     4741 ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc
     4801 tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaaacctt
     4861 caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc
     4921 tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa
     4981 taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat
     5041 cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga
     5101 ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga
     5161 gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg
     5221 gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta
     5281 tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta
     5341 tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt
     5401 ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac
     5461 gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc
     5521 gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa
     5581 attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat
     5641 tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga
     5701 ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt
     5761 atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta
     5821 tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac
     5881 actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa
     5941 gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc
     6001 tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa
     6061 aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt
     6121 tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca
     6181 aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct
     6241 atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg
     6301 gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc
     6361 caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc
     6421 gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg
     6481 ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta
     6541 acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat
     6601 gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct
     6661 gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct
     6721 tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca
     6781 ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca
     6841 gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct
     6901 ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc
     6961 cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc
     7021 gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca
     7081 gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga
     7141 cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc
     7201 cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact
     7261 gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa
     7321 ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat
     7381 gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc
     7441 agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac
     7501 aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt
     7561 tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc
     7621 aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc
     7681 gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag
     7741 atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg
     7801 acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt
     7861 cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta
     7921 aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac
     7981 taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca
     8041 tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa
     8101 tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt
     8161 ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt
     8221 ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt
     8281 gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt
     8341 cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgaccac aattcttggg
     8401 cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat
     8461 ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa
     8521 gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc
     8581 ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt
     8641 ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat
     8701 ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa
     8761 tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg
     8821 tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat
     8881 aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata
     8941 attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg
     9001 aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg
     9061 ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag
     9121 tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta
     9181 ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt
     9241 catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat
     9301 ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc
     9361 ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg
     9421 cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca
     9481 cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa
     9541 gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa
     9601 gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt
     9661 tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt
     9721 cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg
     9781 ctttttctag actgatttct cttggtgttt tcaggagata actaattttg tagttcacag
     9841 cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg
     9901 gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata
     9961 cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa
    10021 tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc
    10081 gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca
    10141 gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct
    10201 aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct
    10261 tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc
    10321 atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt
    10381 caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct
    10441 tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt
    10501 ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac
    10561 ctcggaaatt actttttctt catgataact cccttgttca tctatatagg tcaatacacc
    10621 ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag
    10681 atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac
    10741 gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc
    10801 atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac
    10861 atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca
    10921 agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc
    10981 cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc
    11041 gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat
    11101 aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc
    11161 aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc catattcata
    11221 atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc
    11281 agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga
    11341 aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg
    11401 atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta
    11461 ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta
    11521 aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag
    11581 gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag
    11641 gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga
    11701 aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag
    11761 aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg
    11821 gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg
    11881 ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc
    11941 aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga
    12001 ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc
    12061 tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt
    12121 tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt
    12181 tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac
    12241 gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc
    12301 attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt
    12361 ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat
    12421 tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag
    12481 aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt
    12541 agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga
    12601 agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat
    12661 agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat
    12721 aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt
    12781 ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc
    12841 tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa
    12901 atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg
    12961 atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt
    13021 cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac
    13081 ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt
    13141 ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat
    13201 cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt
    13261 tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt
    13321 ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg
    13381 ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg
    13441 ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt
    13501 ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa
    13561 ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac
    13621 tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga
    13681 tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta
    13741 ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg
    13801 ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg
    13861 tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg
    13921 ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt
    13981 tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg
    14041 atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg
    14101 gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg
    14161 ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag
    14221 ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga
    14281 cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc
    14341 ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg
    14401 tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct
    14461 catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat
    14521 caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga
    14581 tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt
    14641 cttctaaatt atattttcca acttctaaat gattaacgat caactggcct ttttcaatag
    14701 atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg
    14761 ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt
    14821 tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt
    14881 tttcggcagt accgattttt gtaactttca acgttccatc attaccgact gtatttttag
    14941 ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt
    15001 tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt
    15061 ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata
    15121 aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag
    15181 actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt
    15241 cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt
    15301 cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt
    15361 tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt
    15421 ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat
    15481 ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa
    15541 taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg
    15601 acggctattt tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga
    15661 aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc
    15721 ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt
    15781 tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc
    15841 tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg
    15901 tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac
    15961 tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt
    16021 cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata
    16081 tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac
    16141 aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt
    16201 taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg
    16261 aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc
    16321 ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa
    16381 atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt
    16441 ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc
    16501 tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag
    16561 agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc
    16621 tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac
    16681 atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc
    16741 ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa
    16801 agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa
    16861 tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct
    16921 ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga
    16981 tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg
    17041 atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt
    17101 ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct
    17161 tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg
    17221 aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc
    17281 tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt
    17341 agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg
    17401 gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc
    17461 ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg
    17521 agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa
    17581 gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg
    17641 tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca
    17701 atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta
    17761 ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga
    17821 gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga
    17881 gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg
    17941 aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt
    18001 aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat
    18061 cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat
    18121 tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg
    18181 tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg
    18241 tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct
    18301 ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact
    18361 ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc
    18421 tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct
    18481 gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga
    18541 ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc
    18601 cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca
    18661 tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc taggtattca
    18721 gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa
    18781 accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt
    18841 tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct
    18901 ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca
    18961 tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga
    19021 gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt
    19081 ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg
    19141 cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta
    19201 atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg
    19261 tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg
    19321 ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta
    19381 ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct
    19441 ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc
    19501 tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac
    19561 gaatgggtcc gatcattagg atctccattc caagaattta taccatccca ttgatttgtt
    19621 ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct
    19681 ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc
    19741 acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt
    19801 tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc
    19861 ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc
    19921 tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt
    19981 gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct
    20041 tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca
    20101 gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa
    20161 atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca
    20221 gataatggca caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc
    20281 ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt
    20341 gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat gataattatt
    20401 atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa
    20461 gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat
    20521 atgatcaggt tgttggaatt aaactataga aagatggaaa aaatcattcg aattctgtcc
    20581 atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct
    20641 acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa
    20701 tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt
    20761 ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat
    20821 ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca
    20881 cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac
    20941 caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt
    21001 ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc
    21061 aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat
    21121 gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact
    21181 ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac
    21241 ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg
    21301 aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt
    21361 gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa
    21421 aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg tattttccca
    21481 tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc
    21541 cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat
    21601 cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa
    21661 attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat
    21721 agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag
    21781 acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc
    21841 gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg
    21901 tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag
    21961 ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta
    22021 ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt
    22081 tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta
    22141 taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc
    22201 cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg
    22261 atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc
    22321 gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat
    22381 gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc
    22441 atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac
    22501 gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc
    22561 atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat
    22621 ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct
    22681 tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt
    22741 gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt
    22801 taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt
    22861 ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt
    22921 ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca
    22981 gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa
    23041 attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt
    23101 ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga
    23161 tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt
    23221 tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa
    23281 ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca
    23341 tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt
    23401 tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata
    23461 tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt
    23521 caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc
    23581 tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat
    23641 agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa
    23701 gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact
    23761 ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat
    23821 tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg
    23881 ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac
    23941 tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat
    24001 tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc
    24061 attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt
    24121 tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc
    24181 gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc
    24241 tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat
    24301 tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata
    24361 cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc
    24421 tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc
    24481 acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat
    24541 caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt
    24601 taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa
    24661 tgattaaact attagtagta gaagacgata aagtattatc caataatata aaagaaatac
    24721 tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg
    24781 aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg
    24841 aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa
    24901 aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa
    24961 aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag
    25021 gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg
    25081 aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt
    25141 attttttaca aaataaggga atcattttga caaaagagca gatatttgac cggatctggg
    25201 gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac
    25261 atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac
    25321 tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt
    25381 gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag
    25441 atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat
    25501 tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg
    25561 actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac
    25621 cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct
    25681 attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc
    25741 atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt
    25801 acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca
    25861 aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt
    25921 ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt
    25981 attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca
    26041 ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt
    26101 attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg
    26161 acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa
    26221 atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc
    26281 atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa
    26341 aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca
    26401 gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag
    26461 aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg
    26521 gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa
    26581 caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc
    26641 gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc
    26701 tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag
    26761 cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag
    26821 cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat
    26881 ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat
    26941 gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga
    27001 ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg
    27061 ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag
    27121 tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta
    27181 catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga
    27241 tatcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag
    27301 ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt
    27361 ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa
    27421 cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg
    27481 tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg
    27541 gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata
    27601 aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca
    27661 atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc
    27721 gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat
    27781 agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt
//
LOCUS       DAXWLB010000018        25737 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXWLB010000018 DAXWLB010000000
VERSION     DAXWLB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.018"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            212..1123
                     /locus_tag="ACGE4H_000605"
     CDS             212..1123
                     /locus_tag="ACGE4H_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8364045.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE4H_000606"
     CDS             complement(1379..2296)
                     /gene="rnhC"
                     /locus_tag="ACGE4H_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFF8364046.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE4H_000607"
     CDS             2498..2935
                     /gene="zapA"
                     /locus_tag="ACGE4H_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFF8364047.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            2993..3540
                     /locus_tag="ACGE4H_000608"
                     /pseudo
     CDS             2993..3540
                     /locus_tag="ACGE4H_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            3655..6015
                     /locus_tag="ACGE4H_000609"
     CDS             3655..6015
                     /locus_tag="ACGE4H_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFF8364048.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE4H_000610"
     CDS             6102..6416
                     /gene="trxA"
                     /locus_tag="ACGE4H_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFF8364049.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE4H_000611"
     CDS             6598..8376
                     /gene="uvrC"
                     /locus_tag="ACGE4H_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFF8364050.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8521..9420
                     /locus_tag="ACGE4H_000612"
     CDS             8521..9420
                     /locus_tag="ACGE4H_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364051.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9413..10675
                     /locus_tag="ACGE4H_000613"
     CDS             9413..10675
                     /locus_tag="ACGE4H_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364052.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10838..12736
                     /locus_tag="ACGE4H_000614"
     CDS             10838..12736
                     /locus_tag="ACGE4H_000614"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFF8364053.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13035..13754)
                     /locus_tag="ACGE4H_000615"
     CDS             complement(13035..13754)
                     /locus_tag="ACGE4H_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFF8364054.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13944..13991
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14091..14423
                     /locus_tag="ACGE4H_000616"
     CDS             14091..14423
                     /locus_tag="ACGE4H_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364055.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14410..16413
                     /locus_tag="ACGE4H_000617"
     CDS             14410..16413
                     /locus_tag="ACGE4H_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFF8364056.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16432..16902
                     /locus_tag="ACGE4H_000618"
     CDS             16432..16902
                     /locus_tag="ACGE4H_000618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFF8364057.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16923..17507
                     /locus_tag="ACGE4H_000619"
     CDS             16923..17507
                     /locus_tag="ACGE4H_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFF8364058.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17522..18508
                     /locus_tag="ACGE4H_000620"
     CDS             17522..18508
                     /locus_tag="ACGE4H_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFF8364059.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMCYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            18498..18809
                     /locus_tag="ACGE4H_000621"
     CDS             18498..18809
                     /locus_tag="ACGE4H_000621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFF8364060.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18831..20612
                     /locus_tag="ACGE4H_000622"
     CDS             18831..20612
                     /locus_tag="ACGE4H_000622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFF8364061.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20605..21981
                     /locus_tag="ACGE4H_000623"
     CDS             20605..21981
                     /locus_tag="ACGE4H_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFF8364062.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            21988..22623
                     /locus_tag="ACGE4H_000624"
     CDS             21988..22623
                     /locus_tag="ACGE4H_000624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFF8364063.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22653..24008
                     /locus_tag="ACGE4H_000625"
     CDS             22653..24008
                     /locus_tag="ACGE4H_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8364064.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24200..25594)
                     /locus_tag="ACGE4H_000626"
     CDS             complement(24200..25594)
                     /locus_tag="ACGE4H_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFF8364065.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         8460 a         4395 c         5579 g         7303 t
ORIGIN      
        1 attgtttgta gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa
       61 aggagatttc aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct
      121 acggttatat cttttacagc cccttttttg cgtacttaag ctgattttaa gttttgaccc
      181 ttagtctgga tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt
      241 tgcaatcatt atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt
      301 tggacaaacg gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga
      361 tacagaacaa gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga
      421 ctcgctaact accccacttg caactataaa cggcggagag ctggcggagt ttctttctga
      481 aacaaaaaat gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga
      541 agatggaacc aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt
      601 gattgtatta gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca
      661 aacagaagaa aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga
      721 tgctggagca acggtttatt tgacccatac tacagatgaa ttagtacagc ttggagatat
      781 atgcgactac agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac
      841 ggaatatgcg aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac
      901 attagcacag acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc
      961 cactgggaac tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg
     1021 ttatatgaat aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat
     1081 tgctgcaagt ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa
     1141 acaaaaatag ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt
     1201 tgaacaccgt ttaaggaaga ggttgtgagc ctgccgttta actccgcgtc acaaggagca
     1261 atttcaaaaa acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc
     1321 atgagaacac cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt
     1381 atttttttag cagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt
     1441 caagcatagg catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc
     1501 cagctcctga tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa
     1561 agctggcacg actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag
     1621 taacgaagta cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat
     1681 ttgcttccgg cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt
     1741 ttagcagcaa atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt
     1801 ttggttggat ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt
     1861 tttttaagat gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa
     1921 caccaagttt acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta
     1981 gcggaccaaa atagctgcca tttccgacct catcactacc taaaacggat aattgactga
     2041 tattttttgg caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc
     2101 cccatttatc agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat
     2161 aagcggtaat ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat
     2221 ttaacaaata agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga
     2281 taatgacgtt gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac
     2341 gacttttcgt tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat
     2401 agttaagtat gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact
     2461 aaataaaact ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta
     2521 caaagcagtg attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat
     2581 ggatatcgta acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca
     2641 aatggataat gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa
     2701 gaaacaagaa agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat
     2761 caaatttaca gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg
     2821 ggaagtattg aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca
     2881 acagatttta aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc
     2941 ttttatcaaa ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag
     3001 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
     3061 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
     3121 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc
     3181 aaagttagca ttttttgatc aggtatttgc ttttcaccta gatgaagctt tttatgcagg
     3241 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
     3301 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
     3361 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
     3421 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga
     3481 gagaacgcca tttcttacca acaagatgta tgagctttgg taacgaatat tttgaattaa
     3541 ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagtttattt aagaaggctg
     3601 cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat
     3661 aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta
     3721 gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagtga gaaacaaacg
     3781 atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg
     3841 atccctatac ctaaacttga aaatatccgt ccccatatga aacggatcga gatcggagca
     3901 gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg
     3961 aatcgtttca ttgatgatct ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg
     4021 gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa
     4081 gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg
     4141 cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag
     4201 tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa
     4261 gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta
     4321 tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct
     4381 gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt
     4441 caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca
     4501 cgatttggta aagaagtaaa agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc
     4561 ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca
     4621 atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc
     4681 acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca
     4741 ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag
     4801 tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta
     4861 tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg
     4921 caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat
     4981 gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatacaatcg ggcaaatacg
     5041 attaatgcca gcatggagtt cgatgtggat acactaagtc caacgtatcg tttgctgatt
     5101 ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa
     5161 gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc
     5221 acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt
     5281 gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa
     5341 gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg
     5401 gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa
     5461 ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa
     5521 gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa
     5581 ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac
     5641 gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg
     5701 acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa
     5761 tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta
     5821 agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt
     5881 gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat
     5941 cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt
     6001 gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa
     6061 tgctatacta atcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag
     6121 ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt
     6181 ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg
     6241 aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat
     6301 tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag
     6361 ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac
     6421 ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg
     6481 gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt caggctggat
     6541 tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg
     6601 aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg
     6661 aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc
     6721 cgctcatatt ttactggcag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa
     6781 gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg
     6841 ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc
     6901 cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaagt actaaaagac
     6961 aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc
     7021 ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt
     7081 tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat
     7141 acgaaaatcg ttgatgaagt gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca
     7201 gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa
     7261 taccgagacc agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact
     7321 gatttactgg atcgagatgt atttggttat gcgattgaca aaggatggat gtgtgtccaa
     7381 gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat
     7441 aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat
     7501 ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg
     7561 ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg
     7621 aataagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa
     7681 cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt
     7741 atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta
     7801 ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac
     7861 ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc acgagtgatt
     7921 cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat
     7981 gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca
     8041 aaaaacgata aacataaaac aaatgagttg ctattcggta tagatttggc tgtagtaccg
     8101 cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga
     8161 ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat
     8221 gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg
     8281 aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc
     8341 gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag
     8401 taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa
     8461 taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa
     8521 atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc
     8581 tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag
     8641 acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg
     8701 aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg
     8761 ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg
     8821 aaatcaagaa aagagattga accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa
     8881 gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg
     8941 atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta
     9001 gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca
     9061 tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc
     9121 atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt
     9181 ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa
     9241 ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct
     9301 gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac
     9361 cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa
     9421 gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat
     9481 catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca
     9541 acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca
     9601 aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc
     9661 acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga
     9721 taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat
     9781 acaacaacaa ctgactggct tgcaatcgat gcttcgagca agtcaattag gcatttcacc
     9841 agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga
     9901 ttcaaaagga aaaatgacag ttggagaaga taactcgaca gtccaatatg tcgtaagtta
     9961 tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga
    10021 gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca
    10081 aaagcatttt tacggaaaac taacaggtgt tttacttgtt gcattaacgc aaatggcatt
    10141 atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa
    10201 tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct
    10261 attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt
    10321 caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg
    10381 atatatgctt ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac
    10441 ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt
    10501 cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat
    10561 gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtg tatagcgaag gcggattatg
    10621 gagttcattg aaacaatcga tttcaattat gagaaatgaa cgaaaaaaag cataaaatat
    10681 agaaaaatat agaaggaagc tggacattta ttgttcccag cttctttttt gtatagacct
    10741 atttctattt ttttattata ttggatatat atattcattg aaaatagagc ataacaactt
    10801 tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt tagatttact
    10861 ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc tcgaagcaat
    10921 tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg aatttcctgc
    10981 ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt
    11041 ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg cttatccaga
    11101 atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga tccatcgttt
    11161 gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc gaaaaggatt
    11221 gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc gtatcaacgg
    11281 gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg aagaagaaaa
    11341 atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt tgcatatcgt
    11401 gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa tcatggattt
    11461 ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg cgtattgcgg
    11521 ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact atttatttga
    11581 attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc atcaaaccta
    11641 tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa gagagcaaga
    11701 agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag aacatcagtt
    11761 gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta
    11821 tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga cttgttttca
    11881 aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag
    11941 tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt ttttgatcga
    12001 aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct gtatacctgt
    12061 agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact
    12121 gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg caaatgaaga
    12181 tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa
    12241 gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc acgtcgaaaa
    12301 aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttggcaga
    12361 attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag taaaagtaaa
    12421 aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg gaggtctatc
    12481 caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca attcttacgg
    12541 tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg caaaaaaatc
    12601 tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa
    12661 tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc tgttggatta
    12721 tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa aaagcgactg
    12781 caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta aaatattaga
    12841 agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc acgacctctt
    12901 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aacaaacatg
    12961 agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg ctcacgacct
    13021 aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg ttcagatgaa
    13081 tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg catattatct
    13141 ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt taacaaagga
    13201 gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc agggagaagg
    13261 tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg cggggcagtg
    13321 ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg ataaagtgtt
    13381 ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat cggttttctt
    13441 tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat atttgtttcc
    13501 gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg accgcctgca
    13561 agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga catgactggc
    13621 agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg cggtgtatag
    13681 cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc ggcaatgccg
    13741 atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg atttaaaaaa
    13801 atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt actatctgta
    13861 aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa tttgttataa
    13921 taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag ttcttcaagg
    13981 ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt agaggtacat
    14041 actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag
    14101 tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa
    14161 ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg gagataatcc
    14221 aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc
    14281 aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg caagcaactt
    14341 tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt gaaagggtga
    14401 aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga taaaagaaat
    14461 caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg ggatctgtat
    14521 caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac gattattgat
    14581 aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga agctatccaa
    14641 ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg tcaagaactt
    14701 tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa attagaagaa
    14761 gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa
    14821 gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat gtatgcaagt
    14881 aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag tttcaaggaa
    14941 gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc aaaacaagtt
    15001 tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa
    15061 tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa
    15121 accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac agcgttaggt
    15181 cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact tgccgttgca
    15241 gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt tttgcaaggt
    15301 tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac tgtatcagaa
    15361 gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca ggaagtccct
    15421 actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga aatgtacagt
    15481 ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta tcttgtcttc
    15541 ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg gacaatacta
    15601 gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt
    15661 ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg acaggcatta
    15721 ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc
    15781 aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt tggattattc
    15841 gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag tgatggtttt
    15901 gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat gttgataaaa
    15961 aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt atgtatctta
    16021 atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat
    16081 ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt gatggcacta
    16141 ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt catgccgcca
    16201 gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact gaatatgttt
    16261 ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga attttttgga
    16321 aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga aaaatacgtc
    16381 aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac aatgatggat
    16441 tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc aacagcgacg
    16501 attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc agcagcagcg
    16561 ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt gcttccaggt
    16621 acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt aggaaatgat
    16681 atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc ttttacaggt
    16741 ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat tttagcgaaa
    16801 aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac atatgcgatt
    16861 ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag gaggaaaagc
    16921 agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag caagaaagag
    16981 cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc
    17041 aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat
    17101 accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg aatgcgaagc
    17161 aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat tgggatgaat
    17221 ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca ggcaaagtaa
    17281 cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg gatgaatgga
    17341 acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat caagcgggtt
    17401 ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt gtacaagata
    17461 tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga gggatcagtg
    17521 tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac ttttgtccaa
    17581 tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa
    17641 atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg aacagaatct
    17701 tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat
    17761 cgtttggatc tatacgatgt gctatacttt ccataatctg aaggttttga caaaagccga
    17821 gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt cattagaaac
    17881 cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca ttatggacag
    17941 tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa ttgacgtgat
    18001 ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag gatatcaaga
    18061 attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga tggctcgagg
    18121 aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct cgggaagtat
    18181 cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca cgaattttct
    18241 attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa atattgatct
    18301 cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag aagcacaaac
    18361 ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg tagaaagcaa
    18421 aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaatcagaaa caatcagaaa
    18481 gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa gattctgttt
    18541 tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa accgttcgtc
    18601 aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg
    18661 cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct gctatcgtat
    18721 tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa aaaaacgtag
    18781 aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt ttgcaaattg
    18841 gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct gatgccagta
    18901 tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc gagatgcgtg
    18961 gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca ggcgaaccgg
    19021 ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt tcacaaatgt
    19081 ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt aatttcttag
    19141 ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc gaaccaacag
    19201 tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa gaaacaaaag
    19261 ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa
    19321 aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa
    19381 atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa
    19441 acccggatgc acccatgatc actggacagc gagtgattga tacattcttc ccagtgacaa
    19501 aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc
    19561 aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa
    19621 atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat acaggagaat
    19681 cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta gcagctcgag
    19741 aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg ggttatgacg
    19801 ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag atgagcggac
    19861 gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca cggcttgcgg
    19921 aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc gaaggaagta
    19981 taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca gtcacacaaa
    20041 atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc caaaaaagac
    20101 attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa gtagggaagt
    20161 atatggatca tatccttcaa gctgactggg catccatggt caccgagggc atgcgtattt
    20221 tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat tccctttcag
    20281 ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat ttgcagcaaa
    20341 atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa atgttgaaga
    20401 tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg
    20461 agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg
    20521 aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg atgcaaacga
    20581 tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc
    20641 ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc
    20701 cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga agataaagca
    20761 atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc tgtacgattt
    20821 ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt attcgatggt
    20881 ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt ggatattaac
    20941 ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca aacaggtatc
    21001 tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt attttctggc
    21061 tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac ggtactcgat
    21121 tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga agaagctgaa
    21181 tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat
    21241 ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt gactgctgca
    21301 gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga catgaccaac
    21361 tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg aagaagagga
    21421 tatcctgggt atctttacac gaacttagct actttattcg aacgagcagg aagaatccgc
    21481 ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga cgataaaact
    21541 catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt gactagagag
    21601 ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc gcggttaaaa
    21661 gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat gaatcagctt
    21721 tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt aggtgaatct
    21781 gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga aaaagaatat
    21841 gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct agggtgggaa
    21901 ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat gctggacaaa
    21961 tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc gacccgcatg
    22021 gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa attattaaaa
    22081 gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa caaccagctg
    22141 cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt ggcaaatgct
    22201 tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa cgttgatttg
    22261 tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt tgaatatgat
    22321 gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc cgaactggat
    22381 cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac agaaatcgaa
    22441 aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt caatgcatta
    22501 gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat gaagctggaa
    22561 gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg taccactgac
    22621 taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa caaataaaga
    22681 aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt ttagttggcg
    22741 gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat tttatcgatg
    22801 cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg aatacagcag
    22861 agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc ggcggtcttg
    22921 gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc agtctcagca
    22981 cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg atgaagctca
    23041 tgctttacat cctaaaattg gcctttgtga tccaattgat tggtacggtt gctttatctt
    23101 ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg ttccatgcaa
    23161 tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc aatcaccagc
    23221 aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg ttaggcttca
    23281 tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa
    23341 tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc tttttcacag
    23401 agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca aacacctttt
    23461 ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc catatgagcc
    23521 atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct gggtcgacgg
    23581 ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt tcgatgttga
    23641 aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca gtgttgcggg
    23701 cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg attttatcgg
    23761 tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag gtcttctctg
    23821 cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca atagggaaat
    23881 tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg gtcttctctc
    23941 tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa agtgtcatga
    24001 ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga aaataaaata
    24061 atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt tgaggcacta
    24121 cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat aagatttgat
    24181 tcctttattt atcaataggt tataattcat atttttttat tttttctttt acgatactta
    24241 gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg aatgcatctg
    24301 gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta gcaagaaagt
    24361 cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga tagtaaggga
    24421 cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg gctctttttg
    24481 taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg atttgagtga
    24541 tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct tgttctatct
    24601 tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa caaataaatt
    24661 ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa ggaggaatga
    24721 tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata acccatagac
    24781 cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt tgaaacccta
    24841 taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt ctacattcct
    24901 gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg atctctaaat
    24961 gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt gctattctat
    25021 cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca ccggaaacaa
    25081 tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt gctgcagtag
    25141 tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca agatttgggt
    25201 tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca aaagaactaa
    25261 ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg atggactcaa
    25321 agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa cctttctcaa
    25381 ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa tcagctagct
    25441 ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata tcttgttcta
    25501 attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct ggtcgccaag
    25561 ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa aattgtatac
    25621 catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg aggagggaat
    25681 tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt gaagaag
//
LOCUS       DAXWLB010000019        25683 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXWLB010000019 DAXWLB010000000
VERSION     DAXWLB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.019"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            4..636
                     /locus_tag="ACGE4H_000627"
     CDS             4..636
                     /locus_tag="ACGE4H_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364066.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(770..895)
                     /locus_tag="ACGE4H_000628"
     CDS             complement(770..895)
                     /locus_tag="ACGE4H_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFF8364067.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1029..1922)
                     /locus_tag="ACGE4H_000629"
     CDS             complement(1029..1922)
                     /locus_tag="ACGE4H_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFF8364068.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2104..3030)
                     /locus_tag="ACGE4H_000630"
     CDS             complement(2104..3030)
                     /locus_tag="ACGE4H_000630"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFF8364069.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3115..3876)
                     /gene="pflA"
                     /locus_tag="ACGE4H_000631"
     CDS             complement(3115..3876)
                     /gene="pflA"
                     /locus_tag="ACGE4H_000631"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFF8364070.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4120..6351)
                     /gene="pflB"
                     /locus_tag="ACGE4H_000632"
     CDS             complement(4120..6351)
                     /gene="pflB"
                     /locus_tag="ACGE4H_000632"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFF8364071.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6628..9078)
                     /gene="parC"
                     /locus_tag="ACGE4H_000633"
     CDS             complement(6628..9078)
                     /gene="parC"
                     /locus_tag="ACGE4H_000633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFF8364072.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9090..11135)
                     /gene="parE"
                     /locus_tag="ACGE4H_000634"
     CDS             complement(9090..11135)
                     /gene="parE"
                     /locus_tag="ACGE4H_000634"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFF8364073.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11301..11735)
                     /locus_tag="ACGE4H_000635"
     CDS             complement(11301..11735)
                     /locus_tag="ACGE4H_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFF8364074.1"
                     /translation="MSVKNPTQETIFNYLEETKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11862..12500
                     /gene="plsY"
                     /locus_tag="ACGE4H_000636"
     CDS             11862..12500
                     /gene="plsY"
                     /locus_tag="ACGE4H_000636"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFF8364075.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12632..13507)
                     /locus_tag="ACGE4H_000637"
     CDS             complement(12632..13507)
                     /locus_tag="ACGE4H_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8364076.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13589..14380)
                     /gene="codY"
                     /locus_tag="ACGE4H_000638"
     CDS             complement(13589..14380)
                     /gene="codY"
                     /locus_tag="ACGE4H_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFF8364077.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14398..15798)
                     /gene="hslU"
                     /locus_tag="ACGE4H_000639"
     CDS             complement(14398..15798)
                     /gene="hslU"
                     /locus_tag="ACGE4H_000639"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFF8364078.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15812..16360)
                     /gene="hslV"
                     /locus_tag="ACGE4H_000640"
     CDS             complement(15812..16360)
                     /gene="hslV"
                     /locus_tag="ACGE4H_000640"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFF8364079.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16397..17302)
                     /gene="xerC"
                     /locus_tag="ACGE4H_000641"
     CDS             complement(16397..17302)
                     /gene="xerC"
                     /locus_tag="ACGE4H_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFF8364080.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVIYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17500..18876)
                     /gene="trmFO"
                     /locus_tag="ACGE4H_000642"
     CDS             complement(17500..18876)
                     /gene="trmFO"
                     /locus_tag="ACGE4H_000642"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFF8364081.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18845..20923)
                     /gene="topA"
                     /locus_tag="ACGE4H_000643"
     CDS             complement(18845..20923)
                     /gene="topA"
                     /locus_tag="ACGE4H_000643"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFF8364082.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21045..21902)
                     /gene="dprA"
                     /locus_tag="ACGE4H_000644"
     CDS             complement(21045..21902)
                     /gene="dprA"
                     /locus_tag="ACGE4H_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFF8364083.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21958..22725)
                     /locus_tag="ACGE4H_000645"
     CDS             complement(21958..22725)
                     /locus_tag="ACGE4H_000645"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFF8364084.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22718..23578)
                     /gene="ylqF"
                     /locus_tag="ACGE4H_000646"
     CDS             complement(22718..23578)
                     /gene="ylqF"
                     /locus_tag="ACGE4H_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFF8364085.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23838..24236
                     /locus_tag="ACGE4H_000647"
     CDS             23838..24236
                     /locus_tag="ACGE4H_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8364086.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24473..25015
                     /locus_tag="ACGE4H_000648"
     CDS             24473..25015
                     /locus_tag="ACGE4H_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8364087.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25098..25649)
                     /gene="lepB"
                     /locus_tag="ACGE4H_000649"
     CDS             complement(25098..25649)
                     /gene="lepB"
                     /locus_tag="ACGE4H_000649"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8364088.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6769 a         5446 c         4417 g         9051 t
ORIGIN      
        1 attatggaaa ttcaacgtag aaccactaat tttttacatt taggctcttt ttttgcaact
       61 ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc gccttctagt
      121 gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga aaatccgttt
      181 tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg tgcctatttt
      241 tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc atttaaaacg
      301 ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt cttctttatc
      361 atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc actcattttt
      421 agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt tatttacagc
      481 ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac gatccaagtc
      541 gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat gtggcaaaga
      601 aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca taaatgcttt
      661 tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat tattaatgac
      721 tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc taatcagcta
      781 gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt aaaaatctaa
      841 cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt tgcattcctt
      901 tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac tcaaataatt
      961 cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa tttttgctta
     1021 ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata agaaaatgca
     1081 tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa ggccatttcc
     1141 tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt tttttcgaca
     1201 ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac aagtagagac
     1261 tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct gtcaacgata
     1321 acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc ggctaattct
     1381 ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt agccaaagta
     1441 agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt tcctgcagtt
     1501 gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc ttctttccgt
     1561 tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg ttcatcaaca
     1621 gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg ttgcaagtaa
     1681 tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct cttgattaat
     1741 cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa ttcttttttc
     1801 aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt caatgaagaa
     1861 aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga ataatagttc
     1921 atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg taaaaaggag
     1981 gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct ttagtatgtt
     2041 ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca tactaccatt
     2101 tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg aaacgacacc
     2161 tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta ctttttcttt
     2221 tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga caactaatac
     2281 gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta gctctgcttg
     2341 tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa cgttggaact
     2401 gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt tatcacttag
     2461 atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt cagcgattgc
     2521 tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt tgaacaacaa
     2581 tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa ttgttacttg
     2641 gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt ctgcacgata
     2701 gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta cagctaaaat
     2761 atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca caagcatgac
     2821 atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct gaaagtggtc
     2881 taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg tttctttacc
     2941 taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg tatctggatt
     3001 ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat ttctcctatt
     3061 taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg cgacctatcg
     3121 cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt cgacacgttc
     3181 ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt cccacttgta
     3241 ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca aggtttgaat
     3301 aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa ctagtacatg
     3361 gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta aaatattttc
     3421 attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga tgtcaaataa
     3481 cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt cttcatacgt
     3541 aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg cttttttaaa
     3601 tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag taataccacc
     3661 tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat ctgctgtgta
     3721 ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc aacgcatgcg
     3781 gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac cgaaagtttc
     3841 gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct tttctttgat
     3901 ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat ttttcgcaat
     3961 tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg cccctgtaaa
     4021 tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct attcaacgtc
     4081 ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt cgtgtgaagt
     4141 tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa ctgcataacc
     4201 agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat cacgcaataa
     4261 atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt atccatctaa
     4321 gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg aaggattgat
     4381 tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag ctgttgagtt
     4441 caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc ctggcgcaag
     4501 tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt aaacaacatt
     4561 tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt tatgacgttt
     4621 gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac ggtcatcatc
     4681 atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc ctttttcatt
     4741 acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg catgagagat
     4801 cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag ccagctgagc
     4861 tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat tgacatatag
     4921 ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa agtctagtat
     4981 ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt catcgactcc
     5041 gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa agaattgcat
     5101 gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat cagaacccca
     5161 ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga tagcgattct
     5221 gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag tcaagtttgg
     5281 ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat tctttgtaac
     5341 taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga tagggaatcc
     5401 tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta atttcattac
     5461 taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat ccaaatcacg
     5521 ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag caccattttg
     5581 tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt ttgcatttgc
     5641 agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt gcgctaatgc
     5701 acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca ttgtatgata
     5761 accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc catacaatgc
     5821 cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa gccctgtaat
     5881 gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata caccttgatt
     5941 atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg tatagccatt
     6001 gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg cttgtttcaa
     6061 aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat agcctgcatc
     6121 gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt tacgctcgaa
     6181 ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt ctgttgggcc
     6241 ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa agtctctagt
     6301 gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca ttgtatggac
     6361 ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac agcaccctat
     6421 ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt acatatattt
     6481 aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt tttttgcaaa
     6541 cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta atacagatgg
     6601 cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag gagcatcgat
     6661 aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat tcgatgttcg
     6721 atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt gattcatcaa
     6781 ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat tttttttcag
     6841 ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt cttgagcaag
     6901 catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac cttctgctaa
     6961 cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact tcactccagc
     7021 tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca ttccttgatg
     7081 cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag caatcagttc
     7141 atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg tgcgccatgg
     7201 ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga atacaaatgt
     7261 tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt ctacagacag
     7321 attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat cgggtagttc
     7381 atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat ggtccaatgt
     7441 actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt cttctggttt
     7501 ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg tcacactgac
     7561 aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt cgatttcgtc
     7621 ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca attctttttt
     7681 cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg tgatttgcgc
     7741 aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca aacggtataa
     7801 ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt cgactaaatt
     7861 ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt catccagaat
     7921 cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt tggctaaatc
     7981 gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc ttaaaatgtc
     8041 tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga aattgtaatt
     8101 gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat tggcgtcttt
     8161 tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta cttcagctat
     8221 tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg tcgctttatt
     8281 tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca ttgcctctat
     8341 ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt ttttgatttc
     8401 atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat attccatcaa
     8461 tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga tcacttcagc
     8521 taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg atccattgac
     8581 gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat catcaaaatt
     8641 ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac ttaacttgga
     8701 taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac tcccgttatt
     8761 cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc ggaccatcgc
     8821 ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc cgacagattt
     8881 tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg caaataatat
     8941 ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt cttggataat
     9001 atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta attcttgtac
     9061 ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg attgttttca
     9121 tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga gatttcagta
     9181 tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac gtgattttcg
     9241 atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg acgttcagct
     9301 tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc catcgttgtt
     9361 tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag catatatcct
     9421 ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata ttcaatgact
     9481 tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat atatactttc
     9541 ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa tacttgaata
     9601 tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt acagtcttcg
     9661 atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt gatttcttca
     9721 tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc tcgtaaaggt
     9781 agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc agaatcccct
     9841 tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt caattttcct
     9901 gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc tctggcttta
     9961 cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat ctggctcatt
    10021 tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc tactacggta
    10081 cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag aagattttca
    10141 ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc ttccagattc
    10201 ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga tttcgtcatc
    10261 gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt attgacaaaa
    10321 gataaaacat tttctgaata accgtcattg tattgataag cgacctctac ttcgatacct
    10381 tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc attcagataa
    10441 tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg ttcttctcca
    10501 cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc tcttaaccgt
    10561 tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc tggtaaaaaa
    10621 tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt accgtcaggt
    10681 ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat cgttacaatt
    10741 agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc accagaagtt
    10801 ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat cacttcaact
    10861 gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc tgcaacagtt
    10921 atactattgt cttcatggat ggtcacatca atctcactgc cataacctga taatgcttcg
    10981 tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct gtcagtggat
    11041 ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac ttggatggac
    11101 gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc aataatactt
    11161 tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa aagagggtag
    11221 acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct ctatcgatca
    11281 tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag ttcaatctta
    11341 atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc agctgcttct
    11401 ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa aaaatcacct
    11461 gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat tggatcatcg
    11521 atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc caataatgga
    11581 tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat gtaacttgtt
    11641 cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgtttcttc caaatagttg
    11701 aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg cttttctttt
    11761 tattccatct attatacttg tatctttcat tttttcaatt gttcctgtta cctattcatg
    11821 ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg atcttattac
    11881 ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc aaattgtttt
    11941 tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat acatttcgtg
    12001 tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa ggtacattgg
    12061 ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg tttggggtat
    12121 gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga aaagctgttg
    12181 cgacaagtgc aggaatgctt ttaggattta atcctgtatt ttttgtttat tcttgtatca
    12241 tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg atcagtgcag
    12301 tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt ttggctaagc
    12361 ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt tatcggcata
    12421 aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt ggtttgaata
    12481 gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg agtgtgacaa
    12541 atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct tggtaaacgg
    12601 tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa tattttgtct
    12661 tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag tttccactac
    12721 tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc gcttcttttg
    12781 gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt acgctatggg
    12841 aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa accaaggcat
    12901 cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct aacgtctttt
    12961 gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc aatggagaaa
    13021 atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg aaagcaggat
    13081 gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga taatgaaccg
    13141 tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat ggatactttt
    13201 ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca tgttcaatga
    13261 cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt ttcccttgat
    13321 aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt gcatgtctgc
    13381 cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt gatttcagac
    13441 tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt tgaataataa
    13501 cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc tggaacaaac
    13561 atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac tcgtctttaa
    13621 aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga gattcaatga
    13681 ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg atgccgatac
    13741 tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct tcaaagatag
    13801 cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg acagccgttg
    13861 cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc ataccgacaa
    13921 ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt tccatacgag
    13981 ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca gtcgtcagac
    14041 caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta gtaattggga
    14101 tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca ggaaattgtt
    14161 tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca ttataaccta
    14221 gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca cctagtgtga
    14281 cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt tgctgcaaaa
    14341 gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc cgatttttta
    14401 taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt catccacata
    14461 tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata atagatcttc
    14521 tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg tgtcacggtt
    14581 gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg taactgacac
    14641 attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg gttcggtcag
    14701 aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg ggaagcgtcc
    14761 ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg aagcaatgaa
    14821 taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac cttcgacaat
    14881 tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct gtgatttaga
    14941 agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta atttgattgc
    15001 ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga tcaataactc
    15061 taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca aagcacctag
    15121 agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg gtgcaggttt
    15181 cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct tttgtagttg
    15241 ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt gcggttcagc
    15301 ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat tagtggattg
    15361 tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt ctgcattttt
    15421 tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga tcgcattttc
    15481 tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt cagtaaattt
    15541 tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc gtgcgatttc
    15601 tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa tatcttgttg
    15661 cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg caacagattt
    15721 ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt gtcttggtgt
    15781 ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata atattatgat
    15841 tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt tctttcgcac
    15901 tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca aaattccctc
    15961 ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta cctgaaacaa
    16021 gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc atagattgct
    16081 gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg ttatattcgt
    16141 ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt ccggcgaaac
    16201 ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct ttcatgacaa
    16261 cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg tctttttcga
    16321 cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc tcctaaattt
    16381 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
    16441 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
    16501 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
    16561 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
    16621 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
    16681 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
    16741 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
    16801 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
    16861 ctgacccgta aatgacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
    16921 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
    16981 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
    17041 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
    17101 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
    17161 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
    17221 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
    17281 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
    17341 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
    17401 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
    17461 gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg ggattgcagt
    17521 tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg caattgcttc
    17581 atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac catagttggc
    17641 attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca tacttccaat
    17701 agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg cggcgttgat
    17761 tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag tcatctgacc
    17821 tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca attctggcga
    17881 attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat tttctaaccc
    17941 aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct gaaagcccac
    18001 gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag cataaggacg
    18061 tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt
    18121 gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt tttctttctc
    18181 aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag ctttgaattc
    18241 ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa
    18301 atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg catcatagaa
    18361 ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat gagaagtcaa
    18421 aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt cgtttatcac
    18481 tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc
    18541 aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga ttactaccga
    18601 attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac tgtttcctct
    18661 taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct cagtcgattt
    18721 gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct gccaagcagc
    18781 ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa gaacctcttt
    18841 tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact tgtttaccgc
    18901 ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg cccactggtt
    18961 tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat agtctgtttt
    19021 tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc acaccgattt
    19081 cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca taaaacttcc
    19141 catatttccc taattttatc accatcggat gtccacagac atcacaatcg aatcctgctg
    19201 gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt tctttttcaa
    19261 acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct tcaattttat
    19321 ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg aagaattcac
    19381 agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt
    19441 ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta
    19501 ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct ggtggttgag
    19561 taaaatgttg ttttggttca atatcaacag attggactac atcgccttct tcaagttctg
    19621 gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac acttgcatga
    19681 atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt tgttctaatg
    19741 tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa cgtgaccaga
    19801 tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca tctggtgtac
    19861 gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga gcatttttga
    19921 tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata aatgctgcgg
    19981 cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat gtaatcaagc
    20041 cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct accatcatgg
    20101 tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta cttgtagtga
    20161 aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact tcgaagtctg
    20221 ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat ttctttttct
    20281 taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta aaattaccgt
    20341 cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg tcaataataa
    20401 tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc ttacgccaga
    20461 gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct tgctgtgcat
    20521 ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact gcgtcttttg
    20581 tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt aggtacgcta
    20641 aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa actttttgag
    20701 ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt atagaaatat
    20761 aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt aaatctctta
    20821 tatggccaac actggccatt actttatagt ttcgtccaag atatttctca attgtcttgg
    20881 cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa tggactcctt
    20941 tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct aatcatatcc
    21001 attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct
    21061 gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa atgatgacat
    21121 ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct tccaagatcc
    21181 aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa gacacatgtt
    21241 cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt tttcgggccg
    21301 gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc ttgcaagtca
    21361 cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat tgtccgtcca
    21421 ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca gccactgacg
    21481 atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt tttggctaaa
    21541 gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa agatagatca
    21601 ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg gggatatttg
    21661 ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt agcgtgaatt
    21721 tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat cttaatgatg
    21781 tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag tgcccatttt
    21841 ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc tatttgatac
    21901 atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg ctgtttttta
    21961 ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg
    22021 ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga aaccatatcc
    22081 aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag caatgatact
    22141 tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct gctgaatagg
    22201 caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca atttttctac
    22261 tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga ttgtctgctg
    22321 atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat aaagaagttc
    22381 tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct catttttcgg
    22441 caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta cttcatcaat
    22501 gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc
    22561 atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg
    22621 ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat ctattctctg
    22681 tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt ttttgctcca
    22741 tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata tcatgcacga
    22801 ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta atatccatca
    22861 gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga tcgctgattc
    22921 ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca tctaaatgga
    22981 caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct tcatcttcaa
    23041 atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct aaacgaagcc
    23101 actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc ttgcctacta
    23161 gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt gcccgaatag
    23221 cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat gcttctttag
    23281 ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct aatgcaagat
    23341 ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct gccagatccg
    23401 ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc attggattac
    23461 gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca tatttcattt
    23521 tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg atggtcattg
    23581 ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt ttctttttca
    23641 atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg aaagtgaacc
    23701 agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt ttggtttcta
    23761 tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac gaacaaaaca
    23821 gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt gctgatgtca
    23881 atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa ttttctactg
    23941 aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc tctcatgatt
    24001 tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca agtggtgctt
    24061 tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa attgcaaaag
    24121 aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa atgttttcat
    24181 tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag atgtaagcag
    24241 ataactaatc tgacgttttt tataaaaaaa aaacaaaggc ggatgatacg ataagtattc
    24301 tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa agcagtcttc
    24361 tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg tcatgacaca
    24421 ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata ttttgaacac
    24481 tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt tgtcgacctc
    24541 tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc ctgcttattt
    24601 tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg gaccgatctg
    24661 ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg gttcaggaac
    24721 gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg gcttattttt
    24781 atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga ttatgatccc
    24841 agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag atttcaaagt
    24901 aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa taatggttgt
    24961 ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg tttgatttta
    25021 cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc tcaacagact
    25081 gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata atagacaaat
    25141 cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact atcttttgaa
    25201 actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga ttgtcccgtt
    25261 agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga tttcaggttt
    25321 ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc attttggtag
    25381 ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc atctgctaat
    25441 tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac aaccatatca
    25501 ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat gaataaaaat
    25561 ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac aagccagaag
    25621 cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct ttttttcatt
    25681 ctt
//
LOCUS       DAXWLB010000020        24944 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXWLB010000020 DAXWLB010000000
VERSION     DAXWLB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.020"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..531)
                     /locus_tag="ACGE4H_000650"
     CDS             complement(<1..531)
                     /locus_tag="ACGE4H_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8364089.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTC"
     gene            complement(664..1347)
                     /gene="rpiA"
                     /locus_tag="ACGE4H_000651"
     CDS             complement(664..1347)
                     /gene="rpiA"
                     /locus_tag="ACGE4H_000651"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFF8364090.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1666..2079
                     /gene="rpsL"
                     /locus_tag="ACGE4H_000652"
     CDS             1666..2079
                     /gene="rpsL"
                     /locus_tag="ACGE4H_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFF8364091.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2163..2633
                     /gene="rpsG"
                     /locus_tag="ACGE4H_000653"
     CDS             2163..2633
                     /gene="rpsG"
                     /locus_tag="ACGE4H_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFF8364092.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2727..4814
                     /gene="fusA"
                     /locus_tag="ACGE4H_000654"
     CDS             2727..4814
                     /gene="fusA"
                     /locus_tag="ACGE4H_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFF8364093.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            4986..6173
                     /gene="tuf"
                     /locus_tag="ACGE4H_000655"
     CDS             4986..6173
                     /gene="tuf"
                     /locus_tag="ACGE4H_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8364094.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6580..6888
                     /gene="rpsJ"
                     /locus_tag="ACGE4H_000656"
     CDS             6580..6888
                     /gene="rpsJ"
                     /locus_tag="ACGE4H_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFF8364095.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6919..7548
                     /gene="rplC"
                     /locus_tag="ACGE4H_000657"
     CDS             6919..7548
                     /gene="rplC"
                     /locus_tag="ACGE4H_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFF8364096.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7576..8199
                     /gene="rplD"
                     /locus_tag="ACGE4H_000658"
     CDS             7576..8199
                     /gene="rplD"
                     /locus_tag="ACGE4H_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFF8364097.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8199..8489
                     /gene="rplW"
                     /locus_tag="ACGE4H_000659"
     CDS             8199..8489
                     /gene="rplW"
                     /locus_tag="ACGE4H_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFF8364098.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8528..9361
                     /gene="rplB"
                     /locus_tag="ACGE4H_000660"
     CDS             8528..9361
                     /gene="rplB"
                     /locus_tag="ACGE4H_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFF8364099.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9404..9682
                     /gene="rpsS"
                     /locus_tag="ACGE4H_000661"
     CDS             9404..9682
                     /gene="rpsS"
                     /locus_tag="ACGE4H_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFF8364100.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9705..10052
                     /gene="rplV"
                     /locus_tag="ACGE4H_000662"
     CDS             9705..10052
                     /gene="rplV"
                     /locus_tag="ACGE4H_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFF8364101.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10066..10722
                     /gene="rpsC"
                     /locus_tag="ACGE4H_000663"
     CDS             10066..10722
                     /gene="rpsC"
                     /locus_tag="ACGE4H_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFF8364102.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10725..11159
                     /gene="rplP"
                     /locus_tag="ACGE4H_000664"
     CDS             10725..11159
                     /gene="rplP"
                     /locus_tag="ACGE4H_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFF8364103.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11149..11337
                     /gene="rpmC"
                     /locus_tag="ACGE4H_000665"
     CDS             11149..11337
                     /gene="rpmC"
                     /locus_tag="ACGE4H_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFF8364104.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11362..11628
                     /gene="rpsQ"
                     /locus_tag="ACGE4H_000666"
     CDS             11362..11628
                     /gene="rpsQ"
                     /locus_tag="ACGE4H_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFF8364105.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11686..12054
                     /gene="rplN"
                     /locus_tag="ACGE4H_000667"
     CDS             11686..12054
                     /gene="rplN"
                     /locus_tag="ACGE4H_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFF8364106.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12091..12399
                     /gene="rplX"
                     /locus_tag="ACGE4H_000668"
     CDS             12091..12399
                     /gene="rplX"
                     /locus_tag="ACGE4H_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFF8364107.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12427..12966
                     /gene="rplE"
                     /locus_tag="ACGE4H_000669"
     CDS             12427..12966
                     /gene="rplE"
                     /locus_tag="ACGE4H_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFF8364108.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            12985..13170
                     /locus_tag="ACGE4H_000670"
     CDS             12985..13170
                     /locus_tag="ACGE4H_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFF8364109.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13206..13604
                     /gene="rpsH"
                     /locus_tag="ACGE4H_000671"
     CDS             13206..13604
                     /gene="rpsH"
                     /locus_tag="ACGE4H_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFF8364110.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13636..14172
                     /gene="rplF"
                     /locus_tag="ACGE4H_000672"
     CDS             13636..14172
                     /gene="rplF"
                     /locus_tag="ACGE4H_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFF8364111.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14333..14689
                     /gene="rplR"
                     /locus_tag="ACGE4H_000673"
     CDS             14333..14689
                     /gene="rplR"
                     /locus_tag="ACGE4H_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFF8364112.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14710..15210
                     /gene="rpsE"
                     /locus_tag="ACGE4H_000674"
     CDS             14710..15210
                     /gene="rpsE"
                     /locus_tag="ACGE4H_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFF8364113.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15225..15404
                     /gene="rpmD"
                     /locus_tag="ACGE4H_000675"
     CDS             15225..15404
                     /gene="rpmD"
                     /locus_tag="ACGE4H_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFF8364114.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15448..15888
                     /gene="rplO"
                     /locus_tag="ACGE4H_000676"
     CDS             15448..15888
                     /gene="rplO"
                     /locus_tag="ACGE4H_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFF8364115.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15888..17183
                     /gene="secY"
                     /locus_tag="ACGE4H_000677"
     CDS             15888..17183
                     /gene="secY"
                     /locus_tag="ACGE4H_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFF8364116.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17245..17892
                     /locus_tag="ACGE4H_000678"
     CDS             17245..17892
                     /locus_tag="ACGE4H_000678"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFF8364117.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18083..18301
                     /gene="infA"
                     /locus_tag="ACGE4H_000679"
     CDS             18083..18301
                     /gene="infA"
                     /locus_tag="ACGE4H_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFF8364118.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18334..18450
                     /gene="rpmJ"
                     /locus_tag="ACGE4H_000680"
     CDS             18334..18450
                     /gene="rpmJ"
                     /locus_tag="ACGE4H_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFF8364119.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18468..18833
                     /gene="rpsM"
                     /locus_tag="ACGE4H_000681"
     CDS             18468..18833
                     /gene="rpsM"
                     /locus_tag="ACGE4H_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFF8364120.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18861..19250
                     /gene="rpsK"
                     /locus_tag="ACGE4H_000682"
     CDS             18861..19250
                     /gene="rpsK"
                     /locus_tag="ACGE4H_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFF8364121.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19330..20268
                     /locus_tag="ACGE4H_000683"
     CDS             19330..20268
                     /locus_tag="ACGE4H_000683"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFF8364122.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20296..20676
                     /gene="rplQ"
                     /locus_tag="ACGE4H_000684"
     CDS             20296..20676
                     /gene="rplQ"
                     /locus_tag="ACGE4H_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFF8364123.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            20948..21787
                     /locus_tag="ACGE4H_000685"
     CDS             20948..21787
                     /locus_tag="ACGE4H_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8364124.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21763..22632
                     /locus_tag="ACGE4H_000686"
     CDS             21763..22632
                     /locus_tag="ACGE4H_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8364125.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22622..23419
                     /locus_tag="ACGE4H_000687"
     CDS             22622..23419
                     /locus_tag="ACGE4H_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFF8364126.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23622..24368
                     /gene="truA"
                     /locus_tag="ACGE4H_000688"
     CDS             23622..24368
                     /gene="truA"
                     /locus_tag="ACGE4H_000688"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8364127.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24697..>24944)
                     /locus_tag="ACGE4H_000689"
     CDS             complement(24697..>24944)
                     /locus_tag="ACGE4H_000689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364128.1"
                     /translation="KMTNLFRLSSLRAIKTTFHRFLVTEKIGNKKMIHLSTPRPQVTI
                     FLKDEKLFFEIVTCDSELNDRLTTSFLNGVQSADLRY"
BASE COUNT         8135 a         4603 c         5396 g         6810 t
ORIGIN      
        1 acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt cttttttttc
       61 ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc ttccgccgat
      121 ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga tcaatgcgca
      181 ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt cgcgcgtatc
      241 gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt gcgtgagttc
      301 tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta gtgcatcgcc
      361 ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat tttgttccac
      421 ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat caacctgagt
      481 tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca tggtgattcc
      541 tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa agtacgtcaa
      601 aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc aagcagtttt
      661 tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc cgacaactac
      721 tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt catctaaata
      781 ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga tgtaatgacc
      841 accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt ggaatctttt
      901 ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa ctgggagtgg
      961 gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg tttcttttga
     1021 gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga tcccttgaaa
     1081 atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga cttcatcaat
     1141 actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg aagtagtaac
     1201 tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta ccatataata
     1261 ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt tcgcggcttc
     1321 aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt ttgagcatcg
     1381 tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt ttttattcca
     1441 ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat tcgtgatatt
     1501 ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg tttatcaaag
     1561 catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa gaaccacctg
     1621 gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc tacaattaac
     1681 caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc tgctttgaac
     1741 aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca aaaacgtggg
     1801 gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt acgtaaatat
     1861 gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg gattggtcat
     1921 aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga cttaccaggg
     1981 gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga ccgtaaacaa
     2041 agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt aaacaaatga
     2101 aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag gaggagttac
     2161 ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct atttataact
     2221 caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc gggattgcag
     2281 ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac gatccattag
     2341 aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa gcacgtcgtg
     2401 ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc gttactctag
     2461 gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg gaagaacgct
     2521 tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa aaacgtgaag
     2581 acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg taagattctc
     2641 tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt agcaactaga
     2701 tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa aacactcgta
     2761 atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa cgtatcttgt
     2821 actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca caaatggact
     2881 ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact acagcacaat
     2941 ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc acagttgaag
     3001 ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca caatcaggtg
     3061 ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta cctcgtatcg
     3121 tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta tcaactttac
     3181 atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg gaagacgact
     3241 tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac gatcttggaa
     3301 cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa gaatggcgcg
     3361 aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa tacttagaag
     3421 gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca gttaacgtag
     3481 aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa ttactattgg
     3541 acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa gggattgacc
     3601 ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc tcagctttag
     3661 catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt gtgtactctg
     3721 gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt gaacgtgttg
     3781 gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt tacgctggag
     3841 atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta tgtgatgaaa
     3901 agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa gtcgctattg
     3961 aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa ctatctgaag
     4021 aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc atcgctggta
     4081 tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc aaagttgaag
     4141 ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc actcaagccg
     4201 aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta tggattgaat
     4261 ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc ggtggggttg
     4321 ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg gaaaatggtg
     4381 tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt tcataccatg
     4441 atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta cgtgcagcag
     4501 ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt gttatccctg
     4561 aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt gttgaaggta
     4621 tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt gctgaaatgt
     4681 ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt acaatgacat
     4741 ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag aaaaacggcg
     4801 gaaaagctga ataatttctt aaaaccggat agtcctgcaa agagattatc caaagagact
     4861 atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg tggtgtacct
     4921 tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta ggaggacatt
     4981 ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt ggtactatcg
     5041 gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta ctatctaaga
     5101 aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa gaacgcgaac
     5161 gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc cactacgctc
     5221 acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt gctgctcaaa
     5281 tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa actcgtgaac
     5341 acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg aacaaagtag
     5401 acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt gacctattaa
     5461 cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct ttgaaagctc
     5521 tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca gttgacgaat
     5581 acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt gaagacgtgt
     5641 tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga caagttcgcg
     5701 ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca acagttactg
     5761 gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac attggtgctt
     5821 tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct aaaccaggta
     5881 caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa gaagaaggtg
     5941 gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt acaactgacg
     6001 taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc gacaacgtaa
     6061 caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa ttctcaatcc
     6121 gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct taattcactt
     6181 aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa ggtcttagga
     6241 tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg agtattataa
     6301 atagaagaaa cacgaaataa aagagtcagt attttctaaa tgaaacttta tgaaaacaaa
     6361 atgtttttta caagaaaaag ccttgctttg tgagtttata tatattataa tgacaaaggt
     6421 gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga
     6481 gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag
     6541 ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt
     6601 atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa
     6661 actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt
     6721 ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg
     6781 cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg
     6841 atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa
     6901 aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa
     6961 atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta
     7021 gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa
     7081 gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg
     7141 gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga
     7201 aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg
     7261 aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct
     7321 cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta
     7381 tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg
     7441 gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga
     7501 gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa
     7561 aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt
     7621 gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat
     7681 gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc
     7741 gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa
     7801 ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt
     7861 tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca
     7921 gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa
     7981 acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt
     8041 ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta
     8101 gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca
     8161 caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca
     8221 aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg
     8281 aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg
     8341 ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat
     8401 atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag
     8461 aaatccaatt attcgaagct gctgaataag cactaataga taactcatta aattaggagg
     8521 gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg
     8581 ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa
     8641 aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca
     8701 caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt
     8761 tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga
     8821 tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc
     8881 aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg
     8941 tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc
     9001 tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc
     9061 tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga
     9121 acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc
     9181 aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa
     9241 agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa
     9301 aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata
     9361 attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa
     9421 gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga
     9481 aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg
     9541 atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat
     9601 ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga
     9661 cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca
     9721 catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa
     9781 ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa
     9841 acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata
     9901 actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta
     9961 caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa
    10021 gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta
    10081 catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa
    10141 aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa
    10201 cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt
    10261 tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc
    10321 ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc
    10381 aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac
    10441 cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct
    10501 caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa
    10561 ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg
    10621 gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa
    10681 attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac
    10741 gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag
    10801 aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc
    10861 aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga
    10921 ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg
    10981 gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa
    11041 tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac
    11101 caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa
    11161 gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa
    11221 ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa
    11281 gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta
    11341 tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt
    11401 cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac
    11461 caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac
    11521 aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca
    11581 aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt
    11641 ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa
    11701 agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt
    11761 ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa
    11821 gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca
    11881 aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg
    11941 attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa
    12001 ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga
    12061 gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa
    12121 gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa
    12181 gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct
    12241 gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg
    12301 gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc
    12361 cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct
    12421 tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa
    12481 aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg
    12541 ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact
    12601 ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt
    12661 ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa
    12721 ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt
    12781 aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc
    12841 ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca
    12901 acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa
    12961 aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt
    13021 cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca
    13081 gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa
    13141 attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa
    13201 gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca
    13261 tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa
    13321 tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg
    13381 tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac
    13441 gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga
    13501 acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc
    13561 gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca
    13621 aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta
    13681 acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt
    13741 actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca
    13801 aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg
    13861 gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt
    13921 gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc
    13981 acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt
    14041 gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa
    14101 ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa
    14161 actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg
    14221 gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa
    14281 acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac
    14341 aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc
    14401 tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca
    14461 aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat
    14521 ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc
    14581 agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg
    14641 tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga
    14701 ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca
    14761 attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt
    14821 gttgtcggtg acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct
    14881 gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt
    14941 ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa
    15001 cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta
    15061 gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt
    15121 cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc
    15181 ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag
    15241 ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt
    15301 taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca
    15361 tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa
    15421 ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt
    15481 tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc
    15541 ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa
    15601 gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt
    15661 aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta
    15721 actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta
    15781 ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca
    15841 gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat
    15901 taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt
    15961 tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc
    16021 aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc
    16081 aaaacttttc cattttctca atgggggttt caccatacat tactgcttct atcgtcattc
    16141 agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg
    16201 gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat
    16261 ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta
    16321 acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt
    16381 ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg
    16441 caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa
    16501 atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg
    16561 tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc
    16621 aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg
    16681 cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt
    16741 tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct
    16801 ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt
    16861 tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag
    16921 gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac
    16981 tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca
    17041 ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga
    17101 tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta
    17161 aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc
    17221 acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt
    17281 aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga
    17341 gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat
    17401 atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta
    17461 gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa
    17521 gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat
    17581 atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat
    17641 tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt
    17701 tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt
    17761 ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg
    17821 ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc
    17881 attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt
    17941 tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat
    18001 tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa
    18061 caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt
    18121 cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct
    18181 tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt
    18241 aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata
    18301 attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca
    18361 atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca
    18421 aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg
    18481 caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta
    18541 ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg
    18601 ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag
    18661 ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt
    18721 cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca
    18781 atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa
    18841 gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga
    18901 agaatataga agcaggtatt gcacatatcc attctacatt caataacact atcatcatga
    18961 tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag
    19021 gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag
    19081 caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg
    19141 aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga
    19201 ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc
    19261 tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta
    19321 aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat
    19381 tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc
    19441 ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat
    19501 ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc
    19561 ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc
    19621 gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa
    19681 attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc
    19741 ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg
    19801 ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa
    19861 gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg
    19921 acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa
    19981 catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa
    20041 aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct
    20101 gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat
    20161 aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt
    20221 aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga
    20281 aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg
    20341 atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct
    20401 cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat
    20461 ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa
    20521 gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct
    20581 cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt
    20641 gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta
    20701 tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg
    20761 ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata
    20821 ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga
    20881 aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg
    20941 agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga
    21001 tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat
    21061 tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct
    21121 gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac
    21181 gatccgacag atggttggaa tggtttttca aaatccagac aatcagtttg ttggttcgac
    21241 agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct
    21301 agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc
    21361 tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg
    21421 acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga
    21481 agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac
    21541 tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact
    21601 tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg
    21661 tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt
    21721 gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa
    21781 caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc
    21841 aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa
    21901 tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt
    21961 ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa
    22021 gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac
    22081 attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa
    22141 gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt
    22201 tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta
    22261 cttgtattgg atgaaccaac ggcaggactt gatccccagg gtagaaaaga aatgatggag
    22321 atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg
    22381 gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat
    22441 agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc
    22501 gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc
    22561 ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc
    22621 catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt
    22681 ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa
    22741 taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa
    22801 agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac
    22861 tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt
    22921 ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat
    22981 catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat
    23041 tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat
    23101 gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa
    23161 cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc
    23221 agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc
    23281 gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt
    23341 gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact
    23401 gatctttttg agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg
    23461 tttgcaagaa aggatgcgag cagtgtatga caagaaatat tagggtctgt ggcagactaa
    23521 ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaatttta taaaaaaatc
    23581 attattccag ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa
    23641 tcattgcata cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg
    23701 ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct
    23761 ttggttcggg aaggacagat gccggagtac atgcgattgg gcaagtaatc cattttgatt
    23821 atccagagga gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg
    23881 atattgctgt aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca
    23941 aagaaaagac gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc
    24001 attatgcaag ttattttcct taccctcttg atttggaaaa aattcagcgg gcgcttcctg
    24061 atttattagg gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata
    24121 aagtacgtac gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt
    24181 ttacatttag gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt
    24241 tgaaaattgg caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag
    24301 atcgtaatgc agcaggacct acagcacatc ctgaaggact ttatttgtat gaagtgaaat
    24361 atgaataaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga atttacgaaa
    24421 attacttctc aaaattttgc aaaatttcgg cttattttcg aaggagctac ttctggaaca
    24481 ccgtgaatca gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaaacaa
    24541 aaatagctcg tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    24601 caccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    24661 ggaaaaatag ttcctcatat tttgccgttt gttgagctaa taccgaaggt cagctgattg
    24721 aacaccgttt aagaaagagg tcgtgagcct gtcatttaac tctgagtcac aagtaacaat
    24781 ttcaaaaaac agcttctcat ctttgaggaa tatcgtgact tgtggacgag gcgttgataa
    24841 gtgaatcatc tttttgttac ctattttttc tgtaaccaaa aaacgatgga acgttgtttt
    24901 gattgccctt aatgaggaca gtcggaacag gtttgtcatt ttta
//
LOCUS       DAXWLB010000021        24596 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXWLB010000021 DAXWLB010000000
VERSION     DAXWLB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.021"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            123..1097
                     /locus_tag="ACGE4H_000690"
     CDS             123..1097
                     /locus_tag="ACGE4H_000690"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFF8364129.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1267..2667
                     /locus_tag="ACGE4H_000691"
     CDS             1267..2667
                     /locus_tag="ACGE4H_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFF8364130.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2745..3467)
                     /locus_tag="ACGE4H_000692"
     CDS             complement(2745..3467)
                     /locus_tag="ACGE4H_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8364131.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3546..4052)
                     /locus_tag="ACGE4H_000693"
     CDS             complement(3546..4052)
                     /locus_tag="ACGE4H_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8364132.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4175..5974
                     /locus_tag="ACGE4H_000694"
     CDS             4175..5974
                     /locus_tag="ACGE4H_000694"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFF8364133.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6042..7616)
                     /locus_tag="ACGE4H_000695"
     CDS             complement(6042..7616)
                     /locus_tag="ACGE4H_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFF8364134.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLHQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(7871..8380)
                     /gene="mreD"
                     /locus_tag="ACGE4H_000696"
     CDS             complement(7871..8380)
                     /gene="mreD"
                     /locus_tag="ACGE4H_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFF8364135.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8384..9238)
                     /gene="mreC"
                     /locus_tag="ACGE4H_000697"
     CDS             complement(8384..9238)
                     /gene="mreC"
                     /locus_tag="ACGE4H_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFF8364136.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(9393..10031)
                     /locus_tag="ACGE4H_000698"
     CDS             complement(9393..10031)
                     /locus_tag="ACGE4H_000698"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFF8364137.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(10032..10682)
                     /gene="rpe"
                     /locus_tag="ACGE4H_000699"
     CDS             complement(10032..10682)
                     /gene="rpe"
                     /locus_tag="ACGE4H_000699"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8364138.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(10695..11594)
                     /gene="rsgA"
                     /locus_tag="ACGE4H_000700"
     CDS             complement(10695..11594)
                     /gene="rsgA"
                     /locus_tag="ACGE4H_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFF8364139.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFFTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(11759..13828)
                     /gene="pknB"
                     /locus_tag="ACGE4H_000701"
     CDS             complement(11759..13828)
                     /gene="pknB"
                     /locus_tag="ACGE4H_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFF8364140.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(13825..14565)
                     /locus_tag="ACGE4H_000702"
     CDS             complement(13825..14565)
                     /locus_tag="ACGE4H_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFF8364141.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(14578..15936)
                     /gene="rsmB"
                     /locus_tag="ACGE4H_000703"
     CDS             complement(14578..15936)
                     /gene="rsmB"
                     /locus_tag="ACGE4H_000703"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFF8364142.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(15936..16874)
                     /gene="fmt"
                     /locus_tag="ACGE4H_000704"
     CDS             complement(15936..16874)
                     /gene="fmt"
                     /locus_tag="ACGE4H_000704"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFF8364143.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(16867..17358)
                     /gene="def"
                     /locus_tag="ACGE4H_000705"
     CDS             complement(16867..17358)
                     /gene="def"
                     /locus_tag="ACGE4H_000705"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8364144.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(17373..19781)
                     /gene="priA"
                     /locus_tag="ACGE4H_000706"
     CDS             complement(17373..19781)
                     /gene="priA"
                     /locus_tag="ACGE4H_000706"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFF8364145.1"
                     /translation="MILTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(20034..20339)
                     /gene="rpoZ"
                     /locus_tag="ACGE4H_000707"
     CDS             complement(20034..20339)
                     /gene="rpoZ"
                     /locus_tag="ACGE4H_000707"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8364146.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(20344..20958)
                     /gene="gmk"
                     /locus_tag="ACGE4H_000708"
     CDS             complement(20344..20958)
                     /gene="gmk"
                     /locus_tag="ACGE4H_000708"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8364147.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(21445..21729)
                     /locus_tag="ACGE4H_000709"
     CDS             complement(21445..21729)
                     /locus_tag="ACGE4H_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364148.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(21747..22637)
                     /locus_tag="ACGE4H_000710"
     CDS             complement(21747..22637)
                     /locus_tag="ACGE4H_000710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFF8364149.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(22657..22875)
                     /locus_tag="ACGE4H_000711"
     CDS             complement(22657..22875)
                     /locus_tag="ACGE4H_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFF8364150.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(22845..24545)
                     /locus_tag="ACGE4H_000712"
     CDS             complement(22845..24545)
                     /locus_tag="ACGE4H_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFF8364151.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
BASE COUNT         6799 a         5248 c         4544 g         8005 t
ORIGIN      
        1 cttttacata taatacaacc ttgttatctg ttatatttta tagccaaata gcgtgacatg
       61 gggcttgaga aatgttagca taagcatggg aaaaaacaaa tcaatggaag gattgatact
      121 aaatgactgc agctgcagaa aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg
      181 ccgtaggttc tagctatgcc tttgctctag ttactcaaaa tatcgctcaa gaagtaggta
      241 ttgtggatat caatactgct aaaactgaag gagacgcaat tgacttgtct cacgctttgg
      301 catttacttc acctaaaaaa atctattctg ctacttacgc agacgcacat gatgctgact
      361 tagttgttat tacagctggc gcaccacaaa aaccaggtga aactcgttta gacttagtaa
      421 acaaaaacct aaaaatcaac cgcgatgttg tcacacaaat cgttgaatca ggatttaacg
      481 gtatcttttt agttgccgct aacccagtag atattttgac ttactcaact tggaaattct
      541 caggtttccc taaagaacgc gtaatcggtt caggtacctc tcttgactca gctcgtttcc
      601 gtcaagcact tgcggaatta gttgacgttg atgcacgtaa cgtccatgct tacatcttag
      661 gtgaacacgg agattcagaa ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa
      721 tctatgaatg ggtaaaaaac aacccagaaa tcgatgaaga agctatggtt aacctattct
      781 ttggcgtacg tgacgctgct tacacaatta tcgagaaaaa aggcgctact ttctatggta
      841 tcgcagtagc attagcacgt atcactcgtg ccatcttaga tgatgaaaat gctgttcttc
      901 ctttatcagt attcatgaat ggcgaatatg gattgaacga catctacatt ggtgcacctg
      961 ctgttatcaa ccgtcaaggt atccaaaaag taatcgaaat tcctttaagt gacagcgagc
     1021 aagaccgtat ggctgcttct gctaagcaat taaaagaaat tctagatgaa gcttttgcta
     1081 aactagacgc tgaataatct ttgaacatga cggaaaacat ctattcggtg ttttccgttt
     1141 ttttatttta tcacccttat ggtttctaaa ttttttcata aagtagagat aaccttcgtc
     1201 ttttcctcta aattatgcta tcatgagacc gttccaccct aaacttttac ttaaggagtt
     1261 gtcgatatga caagatcttc gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt
     1321 cttggtgttc tcttagtgct gatcagcttt tatacctatc gcagtacata ctacacaaag
     1381 cattttttgc cgaatacaga aataaatgga ataaatgtca gcaatctaac agtcgaaaaa
     1441 gcaaatgaaa aattaaaaga agcttattct gataaaaaac tggctattaa agaagatgga
     1501 aaagtctggc aggaagtacc aaaatccgag ctcggctaca aagatgattt cacttcggag
     1561 ctttctccga tcctaaatga acaaaacgga tggacttggg gaatgactta tgtctcagcg
     1621 gcagaaaaac aagaaataga tcctgtcagt caaaaccacc aaaaactaga cactaccgtt
     1681 cagacattga caactaagct gactgatttg aacaaagacc ggacacctac tactgatgca
     1741 acgatcgaaa aagcaggtga ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc
     1801 gacgtcgatg ctgcggtcaa acaattaaaa agtgcggtaa actctggtaa agatacaatt
     1861 gaattgactg aattcaaaga aaaaccaaaa gtaacatctg aagacagctc actgaaagaa
     1921 caactcgcat ccatgaatgc tatcgcaaat gtaaaagcta cttacagtat aaacggtgag
     1981 actttccaga tcccatcaag tgatattatg agttggctga cctacaatga tggaaaagtc
     2041 gatttagata cagaacaagt acgtcaatat gtaacagatt taggcacaaa gtataacaca
     2101 agtacgaatg acacaaaatt taaaagtaca aaaagaggag aagttacggt cccagttggc
     2161 acttacagtt ggacgatcca aacagacagt gagacagaag cattgaaaaa agcgattctg
     2221 gcaggccaag attttactcg ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca
     2281 ttgatcgaag atacgtatat cgaagttgat ctggagaacc agcatatgtg gtattacaaa
     2341 gacggaaaag tggcccttga aacagatatc gtttccggaa agccaactac acctacacct
     2401 gctggagtct tctatgtttg gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac
     2461 gatggaacac cttacgagag tccggtaaac tactggatgc cgattgactg gacaggtgtt
     2521 ggtatccacg attcagactg gcaacctgaa tacggtggag acttatggaa aacccgcggt
     2581 tcacacggct gcatcaacac cccaccaagt gtaatgaagg aattattcgg gatggtcgaa
     2641 aaaggaacgc ctgtattggt cttctaacaa aaataatata taaaaagaaa gcacccgtac
     2701 gattggtaca ggtgctttct ttttattatc taacaatgtg ctacttacat ttcagcagca
     2761 gtaaagactt ctgaaacatc atcatcgtct tctagcttat ctactagacg ttccaattgc
     2821 tctttttgtt cctcatttaa atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc
     2881 gccaaagtga actctttttc caactcatct cgtacagcag caaaatcttc tggtgcagta
     2941 taaatctcga atacttcagg agaagtttgc agatcttctg cccccgcctc taatacatcc
     3001 tcaaacatcg tctcttcatc aacagtcaac ccatcgcgtt caatagcgat atatcctttg
     3061 cggtcaaaca agtaactgac tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa
     3121 gctactcgga catttgtcgc tgtccgatta cggttatctg tcaatgcatg gactaagatt
     3181 cctactccac ccggaccata tccttcatac gtgatttcat cgtaattcgt ttcatctacg
     3241 gcactcgtag cctttttaat cgcccgttcc acattgtcgt taggcatatt tgcagctttt
     3301 gctttgtcca caacaagccg caaagctgga ttcatagatg gatcaggacc gcctgcttta
     3361 gctgccatat aaatttcacg agaaagtttc tgaaagactt tccctcgttt tgcatcttgg
     3421 gcattttttc ttccttggat attatgccat ttactatgtc ctgacatgat ttttcctccc
     3481 ttgttctttt gagaatacac ttctattcta gtactatttc acaaaaaaac aaagccacag
     3541 ccaatctata aacgcttctt gaatgccgca aaaagcacac ataatatcac agcagtcaga
     3601 gcaccgatcg aatccaacat gacatcttgg aacataggtg ttcgtccacc ggtcatcatc
     3661 tgatggaatt cgtccaaacc tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc
     3721 agccaccaaa tttttagctt ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa
     3781 taggtaccga aatgtgcgcc cttccttaaa aagaactcta aaaatttggc gtagcccaga
     3841 tggtcgatac tcaccatact tccaccataa ttaaaacgta tacccgctaa tgtgtcttta
     3901 aatggctgat taggcagcca gctttctaat cgcgaaactt gtgattgctg tccgtaagtt
     3961 tgagaagaac tgtaaaataa gattataacg actatcagtg cgattactaa ataaatattt
     4021 ccgtttttca tttgtttttt tgaagaattc ataagtcacc tctgtatttt atcttagact
     4081 ttttttgaag aaacaacaat cattttcata gagacttctt ccataaaaaa gtgctatact
     4141 taattttgta ttacaatatg aagggagttt tttcatgagt tattcattaa attggaatct
     4201 agattctatt ttttccggag gcagtcattc tgatgctttg aaccaacgga tgaagcaatt
     4261 ggaagaccaa atgaacgagt attatcaccg cgtaacaaaa tggtcaccta gttctgacaa
     4321 tgcagagcag ttaaacgcta ttcttcaatt gcaagaaacg attacgaatg gttttaccca
     4381 atgcagcagc tatattactg cattgctttc tgccaatgtc aatgattcag atgcaaaggt
     4441 cttatcaggc aagctttacg caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc
     4501 aaagaaattt gcagaaatat ctgacaatga ttggaatcag atgctttctc aatcttcgtt
     4561 tgaaacaatt gcttttcgtt taaacgagat tcgtcgtgat ggctcacaat tattatcaga
     4621 agctgaagaa aacattataa atacgctttc tttagatggt ctaaatgctt ggagcagtca
     4681 ttatgatacg attgtagcca gcatttcgat tcccttcgaa caagatggac aagtagtcga
     4741 attatctgct ggacaagcct tcaataagat gatgggcgat ccagatccaa aagtcagaga
     4801 aacacttttc gcagcatggg aagaagcctg gaaagaaaag gcacccctct tcacggatac
     4861 tttgaatcat ttagatggtt ttagactttc tgactatgaa cttcatggtg tcacagattt
     4921 tctacaaaaa ccattagagt acaatcggtt gaagaaagaa acattgtctg tcatgtggga
     4981 tacaatccag aaaaacaaac aaccccttgt taattacttg actagaaaag ctaatctttt
     5041 cggtaaagaa aaaatggaat ggcaagatca agatgcacct attattttag gcgatttgaa
     5101 agaaaaaacc tttagttttg acgaagcagc agcatttatt atcgaaaact ttaacaaatt
     5161 cagtccaaaa atggctaaat ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga
     5221 agatcgtcct gggaaacgtc ctggtggtta ttgtactgag ttaccagaaa caaaagaatc
     5281 aaggatcttt atgacctaca gtaattctgt taatgaggtt gcgacacttg ctcatgaact
     5341 aggacatgct ttccatagca gcacgatgtg ggatttacct tctttaaatc gcgaatatgc
     5401 catgaatgtc gcagaaacag caagtacatt cgcagaactg atcgtagccg atgctacatt
     5461 aaaagtcgca aaaacaaaag aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc
     5521 tttagctatg ttcatgaata tccattcccg attcattttt gaaaatcgat tctatgaagc
     5581 acggcaagaa ggattagtct cagaagataa gatcacagag atgatggttg aggcgcaaaa
     5641 agaaggctat caaggagctt tagctactta ccatccatat ttctgggctg caaaattgca
     5701 tttctttatt gatgacgtac cgttttataa cttcccatac acatttggct acctctttag
     5761 tttgggcatc tatgcttacg ccaaccaaaa gggagcaaat tttgaagaag aatatatcca
     5821 tttactacgc gatactgcct caatgacgac ggaagatctt gcgaagaaac atttgaatgt
     5881 cgatcttacc caaccagatt tttggcaagc aggtatcgac atggtgactg aagatatcca
     5941 aacgttcatg gaattgacag aagaatttat ctgatttcta ttttatcaca ataaaaaaag
     6001 agttagtcca gaatgggcta actctttttg attaatcatt cttacatgct gacagcaaag
     6061 tcaggggcaa accattgaac tgaaccaact tttacgcttt cgcctggttg aggagcatgg
     6121 atatattgtc ctccgcctaa agcaatcgct acgtggtaag ttccgccttg tgacccccag
     6181 aataacaagt ctccagcttt tgcttgagaa actgaaattt ttgttccagc tgattcttga
     6241 ggaactgtcc aaccaccgat gtcacgacca gttacttgca tgtaaacata gcgtgtgaac
     6301 cctgagcagt caaagccact tggatcttta ccgccccaaa catatggagt accaatatat
     6361 ttgtaagctt ctgctacgat tgctgcacca ttcacacttc cagaaggagc tggagcaggt
     6421 gttacaggtt ttggttgttc aggtgttggt gttggcgttg gagccggagt actacttcct
     6481 gtgccgtttc ctgtatcaac actttgatct gttgatggtg ttgtaggcgc aggtgttgtt
     6541 gattcttctg ttgttgaaga ttcaggcact gttgttgatt cttctgttgc tgaagattca
     6601 ggcactgttg ttgattcttc tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt
     6661 gctgaagatt caggcgctgt tgattcttct gttgaagatt caggtgttgt tgattcttct
     6721 gttgctgaag attgtgtagc tgatgatgat tcttcagtca ttgaagattg tgtagctgat
     6781 gattcttctg ttgctgagct ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc
     6841 tctgcttgtt cgcgtgcttc tttttcagct ttttcttgtg cagcttgttg acgagcttgt
     6901 tctgctagac gttgttgttc acgaatacga gcttgctctg cttcagcttc tgctttttga
     6961 cggttcaagt cagctttctt gtcttcagca gttgcttgtt cagcagctaa tgaagttttc
     7021 agtacgttca aatcagcttg tttagataaa agatcgcctt tttgagattc caaagcagct
     7081 tggttttcag ctaattcttt tagtttagca tcgttttcag cttttttgtc ttctacagct
     7141 tttttatctt gtttttgttg ttccatcaag tcgttgtttg cttttaccat cgttgtcatt
     7201 gcttgaacgc gtccaatagc atctgctaat gaatcagcat tcaatacagc atcgatgtag
     7261 tttgagcttg tgttgcttac ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt
     7321 ttttcaatac gttcttgtaa atctgcaatg tcttttacta attgtgcaga ttcttgatgc
     7381 aatgtatctt gttttgctaa taggtcttgt gcttgtgtgt tgatttcaga aacttggctt
     7441 tctaaggcat cgatttgtga ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt
     7501 tgatcttgct gttgtatctg agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct
     7561 acggcagtca aggtcattga acataccatt actgctgata ttaaactctt tttcactctt
     7621 cattcctccg actggcttaa tttaataaat atctaaatat atttaagtct ttcttaacga
     7681 tataaaaatt gtaccatagc agtgtgtcaa cgatataata gttatgttac aaaaatgttt
     7741 caaaacaact tttaaaaatg acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg
     7801 cgttaagtct agcatttctt aagataacaa cgattctttt tcgctttcga cctaaaaata
     7861 aaaaaagaaa ttattcatca ctgaataatt tcttaaaggg aaaaatgaat aatacaaata
     7921 taatcatgtt caacagcagt gtaggtccta aaaaccttgt aataaaatac gtgttattta
     7981 ctactgctaa tttaaaaatc aactggacca ccatcgtaaa caattcataa cccgtgacaa
     8041 agatgatcat actgaaaaat tcagtaaaga tgttgacatg gataacgtct ttcattacat
     8101 acatcaacca tacaattaaa gggagagcta ctgcatagat accgataacg ccaatatagt
     8161 aagcgtcata gatagccccg agaacaatcg tggtaatcag taaataacgt ttttcaaacg
     8221 ccatactgca acataataat gccaatatca gaaagtgggc atttgacata taggtgcctt
     8281 tcgaccactc ccctagcatt cttgttaaat gaccatctat caacatcaaa aagaacaaaa
     8341 cgattggcag atagtacttc attgtttctt tcttcaacat ggactaatcc tccgctagcc
     8401 gttggacaat cgtcacaact gacacgtcat acatttctgc ataaggtttc acataaactt
     8461 cccgatccag cccataacta tctggtttcg ttttaataac cgtaccgatc ggcaagttag
     8521 ctggagaatt ccctccaaga ccggatgtct ggacaacatc cccttctttg atatccgtat
     8581 caccagttaa ttgggtcacc actaaggcat ggagcttttc atcatagttt ttaagcaaac
     8641 caaacgcttc gccattagcc gaagataccc gtactggaaa atgattggag ctttcattag
     8701 aggatgacag taattcgatt ttagacgaag ccgtattgac ctcgatcact cggccaatca
     8761 atcctttttg cgacatgact gccatatttg cttcgatacc gtctttagaa cccttgtcta
     8821 cgatcagcat gtcctgccaa ctgtcaggcg aacgagtaat gacatttgcc gttacttttt
     8881 cgtaactagt cagcgtctga ttcaactcaa gttctttttt caacgcttcg atttctctct
     8941 cgtaattctt gttttgctgt gaaacgtcat catagctatc gattttttct tttagccgct
     9001 cattttcggc ataagttgaa aaaagattat ggacagaatc tactccattt cgtacccacc
     9061 gtactggggc agagattgtt ttatcaacca ttgcaacact gtcattaacg atggattgga
     9121 agaaattggg ctttccttca ttcgctcgct gcgcagcggt cacacttagt atcgtcacca
     9181 cgatgataac aataaccaag gtaataataa tatttttatt aggattgaac ttcttcacaa
     9241 agacacctcg aattaattag actcttgtgt tcaattttat cactaaatgc cgtgaaacaa
     9301 aaggtaagtt gctcctctta agcttttttt tagcaaataa tagattctgt catttttagc
     9361 ctgaaagaca aattttttta tttgtttatg gatcaatctt tgctttgaat aactgcaatc
     9421 ataccatctg aaaaagaaaa ttctgcggta tctgaaagaa attcgttact tgcataagat
     9481 gtcggatatt ctacttcttt attttccaat acatacttgc tgccttttaa tgtaagattc
     9541 gagacgggag tcaagcagca ataagctaaa tacttcatat ctggtatctg tcttactata
     9601 tgctctccag gcaaataata agacaaataa ttttgacaat ctctgataat aagctgatgg
     9661 gcaaagggtt tgaagcgagg ttccatacct aaccagagat tggctaaaag atgatccaat
     9721 cttccgcccg tagctccaat caaagtgact tccgcttgcg ggtaacgttc aaatgttttt
     9781 aagataccta actgtgtatc tgtatcgtct ttttctgcag gggaacgggt atactctttg
     9841 gcttttgcaa atacttcctt ctgctcttcg ttcgacaatg aatcaaaatc gcccactgcc
     9901 agatctactg ggtagcctgc ttgaagcagg tacagcgcac cacggtctat cccgacatag
     9961 gcatcgtatt tggacagcag agactgcggc cagttttgcg gattacctcc agcagctaat
    10021 aaaattttca tttagtctaa tgcctctttt aatcgttcga tttgtccgac tggattttcg
    10081 gctccataga tatatgatcc tgcaacgaag acatccgccc ctgcatcttt acagattttt
    10141 gctgtttctg gaacgattcc gccatctact tcgattgcat aagtccaatt gttctctcta
    10201 cgtagatcag cgagttccgt aattttttct actgtttctt caataaagct ttgtccgccg
    10261 aacccaggat tgacagtcat gactagtact tgatcagcca aaggcaaaac atgtttgatc
    10321 atggagactg gtgttcctgg attgataact actccagctt taactcctaa gctttttgtc
    10381 atttgaagtg cccgatgaat atgaggagtt gcttcgacgt ggacagtaat gatatctgct
    10441 ccagcttttg caaatgctgg aatgtagttt tctggatcaa cgatcatcag atggacgtct
    10501 aacggtaatt tagtaacagg acggattgcc tgaacaatat ttggccctaa tgtgatattt
    10561 gggacaaatt ggccatccat tacatctacg tggatgtaat ctgcgcctcc tttttccact
    10621 aactcaatgt ctctttgtaa attggcaaaa tctgcactca aaatggatgg tgctatcttc
    10681 attcttgttt cctcctattt tcgtttacct tctttttttg catacattgg ttttcttttt
    10741 tcgatttctt gtaagaattg caaataattg tcgtaacgtg tctgtgcgat accacctgtt
    10801 tctactctat gttttacttc gcagccaggt tctttagcat gcatgcactc tctaaatttg
    10861 caatggacag aagcttctac aaattcagga aattgtctcg gcagttctcc tgcttcaatt
    10921 gtcaaaaagt caatcgagct aaatcctggt gtatctgcta ctaacccgcc atagatagga
    10981 atcagttcca catgacgtgt tgtatgccgt cctcttccaa gtgcatcaga aatctctcct
    11041 gttgctaaat tcagttccgg tgatatttga ttcagtagcg tcgattttcc tgctccagac
    11101 tgacccataa agaccgatag ccgttctggg aagtaacgga tcagttctgt cgtatcaccc
    11161 gtatgattgg gtacaatcac cggatagcct attttagtat aggtttgctt gatctcttcg
    11221 attttttctg ggtcgtctga caagtcggtc tttgtaaaaa aaataatcgg ttcgatcgac
    11281 tcgtattcca aggtaactag aaagcggtca agtagattat aagaaaatgc aggctctacg
    11341 atacttgtga caatgattcc aagatcaaca tttgtgactg gtgggcgaac taattgattt
    11401 tttctcggca agatttccag aagatagccg tctgtttgat tttcactctc aaagatcacc
    11461 tgatctccga caagcggcgt gattttccga ttccggaaat ttcctcttcc tcttgtttga
    11521 taggtctctc cgtttgcgta tacatagtaa aaaccgctga ttgcttttct gatttgtcct
    11581 ttgataagtg ccacctccgt ttttgtttat tttaccatac gaagaggaaa gtaggtgtta
    11641 ttccatgcaa acttctcaaa gtatatggct tgaaaattga tcaaaagaga tagaaacaca
    11701 aaaaagagaa tgcaacaaat caatgtcgca ttcccttctg atatatttta ttcagcactt
    11761 attttgaact ttctgaggtg ctagctgatg aacttgtgga gctggaagat tcttcttttg
    11821 aagagttgct tgtgctactg gagctacttg agctgctaga actgtctgaa gagtcttttt
    11881 cagaactaga agacgctgga tctggtcctt tagagtaaat tacgttcaca accattcctt
    11941 tggtaattgt cgttcctgcc cctggtactg tacgaatcac tttatcttta tccaccgtgt
    12001 ttgaatattc ggaagtctca tgcccgatat attctgcacc gacactagct aaataacttt
    12061 gtgcttcgct ttttgtatag ccggagatat cattcaattt aggctctgtt ccttggctta
    12121 cgacaaaatt gatgtaagtt tcttctgcta caacttcacc cgaggctgga tcctgactga
    12181 tgatcgaacc tgatggtaca gtgtcgctat agtcataagt agcactaata ttattgatat
    12241 tgaatccttg tgtaacaagc caattacgaa cagtttcgta gttttctcct acatgactac
    12301 tcaaagtcac tgtttctggt cctttgctga ctgtgaaagt aatcgtgtct gttttaggat
    12361 cgacttctgt atcttctgat ggagattgcg aaatgatcat tccgctttct acctctgaac
    12421 tgaattcctc ttctttttga atcaagtcag cagaaaaccc taattctttc aaacttgctt
    12481 cggcatcttc ataactttta ccactgtaat catctaattt gatttttttc gtgccgctac
    12541 taatatacag agtgacttcg cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct
    12601 tcactacatt accttcttcg attttgtcat ctgcaatttc ttctgttttt gttgctactt
    12661 ctaatttcgc ttttgcgagt gtttctctag cagaagcttc agaaagatca gaaacatcag
    12721 gaacaacaac ttctcctcgc ccgccagaag caaagaatac agctgttccg attcctaaaa
    12781 cagcaagaat agctaatagg atcagccacc attttttctg tcctttcttt ttaggatcag
    12841 gcagaacttc ttcctcctct ggtaaggttt cgttttctgg cttgtctttt ggcagaggca
    12901 tagatttgaa tgaatcaggc attggtgcat cttccgtaag gggtgtgata acttttgttt
    12961 cattatccat cacatgaggc tgccattttt cttcgtttgc acgagctggt gataatactg
    13021 tagaaagatc atcagacatt tcctcggctg acttataacg gtctgcaggt tctttagccg
    13081 ttgcccgtaa aatcacgttt tccagtgctt gtggcacatt tggatcaagt gcttttatag
    13141 atggaagatc atcttgaaaa tgttttaaag caattgttac agcagattca ccatcaaacg
    13201 gtacgctacc cgtcaacatc tcataaagga tgatccctaa agcataaata tctgactgct
    13261 tcgttgccat acttcctctg gcctgctcag gggacagata atgaacagat cctagcatcg
    13321 tattcgtctg cgtgatcgat gtttcagata aagcgatagc aatcccgaaa tccgtgattt
    13381 ttacaacacc ttcattatcg atcagtacgt tttggggttt caaatctcgg tggatgattt
    13441 gatggttatg agctaaagaa atagcagaaa ggatctgctg cataatattt actgctgttt
    13501 cataaggaac aggataatgt gtttggatat atcgtttcaa gtccattcct ttgacgtatt
    13561 ccatcaccag atactgcatg ttgtcttctt caccaacatc gtaaacacta acaatgtttg
    13621 gatgaacgag ttctgttgct gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt
    13681 cattttgaaa gtcaaatcgt aagactttaa ctgctacatc acgatccaag attaaatcgt
    13741 gcgccaaaaa tacatttgcc ataccaccgc taccgatatt tccagtgatt tgataacggc
    13801 catttaattt tttgcccaac tcgatcatac actgtcctcc ttatattcga taagaagaac
    13861 ggtgatatta tccgctccgc ctgcttcatt ggcacgatca atcagctcag ttactttatg
    13921 agacaatggg ccttcttgat tgatgatcgt tcctatttct tcttcagaca gcatgttcgt
    13981 caagccatct gaacataaca gcagtcgatc ttttagttgc caaagatgag tagaaacatc
    14041 tacttcgact gttccaggca tgccgacaga acgtgttaaa atatttttcc ttggatggtt
    14101 cactgccatt tcttcgctga tttctcctga tttgactagt tcattgacta aagaatgatc
    14161 ctccgtcaat tgcatgattt tatcttcacg aatcaaataa gctctgctat ccccaacatt
    14221 ggcaatcgtg aaccagtcat ctgacagagc ggccgctacg attgttgttc ccataccgtt
    14281 atattccggc tgttcttgtc cacgctgata aatcagcata ttttcttctt gaatcgattg
    14341 aatgaaccat tgggcgattt tttcatcgtc aacagcttct tgtttttccc aagcttcgcc
    14401 tagattcgtt accgccatct gactggcaat gtctccggca cgatgaccac ccatcccgtc
    14461 tgctaataca gcaagcttga gacctgaacg gctggtaaat acactggcat agtcttgatt
    14521 tgtgttacgt cgtcttccta catctgattg atattcaatc tgcatttttt cacctcatta
    14581 ctttttgcgc aaacaacaaa tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt
    14641 caacattttc tcttcgattg atgattgtag cataggattg gtcaaaacat caatcctctc
    14701 aaaatcaggg tgtctttcca agaaagcaga aacaacgtct tggttttctt cttttaagat
    14761 cgtacaagtg ctataagtca gaataccgga agactttaaa gttggtgcgc agctttctaa
    14821 aatttccaat tggatttttg gtaaattcgc taattcatcc ggctgttttt tataacgaat
    14881 atctggtttt cgccgaagca gcccaagacc tgaacaaggc gcgtctacta aaatccgatc
    14941 aaactgctct gcttcgaact gtttactgac ttctctagca tccatctttt gagcgtagac
    15001 agcagaagag acacctagac gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg
    15061 tacatctaaa gcgaccactt ctccaccgca tgacggatct aaaaaagaag caatatgcgt
    15121 cgtcttacct ccgggtgctg cacaggcatc taagacatga tgttcaggct cgatttgcat
    15181 agacggcgct acaagcatcg aactttcatc ttggatcgtc atgacgccat cgtggaacaa
    15241 tgagctagaa gctaaatgcc cttttttcgc tactataccg tatggactga cttggctttt
    15301 ttcagctgtg atctcttctt cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg
    15361 tgtatctact cgggcactag catggctagg ctgatatagc gataaaccga gcttttcagt
    15421 ttcttcgtat ccgatttgat ccaccagtcg tttagtcatc catagaggca ggctgatttt
    15481 gacagaaagt ctttcgatag gatcagcaat gccttgcaga tctggtgctc cttgacgctg
    15541 ataattcctc aagacaccat ttacaaattt ccctgtccca ggatttcctt ttacttttgc
    15601 gatttcgacc gcctcgttta aaatagcatg tgcagggact ttatctaaaa attctaattg
    15661 gtatacagaa agataaagaa gcgtcttgac ccagtcatcc actttttttg ctttagcgat
    15721 caatggtgca acgtaaaatt ccaatagtcg ctgatggcta atcgtaccat agatcaattc
    15781 tgtaaataaa cccacatctt tttgatttag ctcgctttgt ttgatcatct cattcaacaa
    15841 aagattcgag taggcgccac ccttgttcac tcgctctaat gtcagtaatg ccatatatcg
    15901 tactgatttt ttcatttttt tagggatttt tttcattatt tcacctgctg accaatctga
    15961 acttgctgac cgcttccgtt aaggaaatca tgtacggcct gttttccttt accagctggc
    16021 tgtaattcat tgatggctaa gacggtctgt ttaccgcaag caatccatag attttttttg
    16081 tctttccgga tgatcgtacc aggtgctttg tctgtagttt ctgccaatac ttcaaccgat
    16141 aataatttcc agcgtatgcc ctcataggtc gtgaatgcaa ttggccaagg acgcattccg
    16201 cgtacttggt tgtcaatttc ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt
    16261 gtgatgtttg gcgaaaaagt tgcttctgac tcgtcttgag gtactggttt ttgtccatcc
    16321 aaaatattgg gcaatgtttc tagcaatagc tttcttccta aaaggctaag cttttcaaac
    16381 atcgtcccta catcatcttg tttagtgatt ggtatgcttt cttgcgcata gattccgcct
    16441 gcatccattt ttttgatcat ttccatgatc gttacaccag tttctttctc cccattcatg
    16501 atggcataat gaacaggcgc acctccccga tatttaggaa gtaaagaagc atggacattg
    16561 attgccccta attttggtac ttgcaataac ttttcaggca agaattggcc aaatgccgca
    16621 gtgacgatca catcaggcac caattcaatg atttcttcca tttctggcga accggagatt
    16681 ttctctggct gcagcacacg gataccgtgc ttcagagccg cttcttttac cggtgtcggc
    16741 gtaattactc gtttccgccc aactggacgg tcgggctgtg ttaccactgc ctggatctcg
    16801 taacctgatt ctatcagccc ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat
    16861 actagcttag tcatcaagat gttcctccat atattcttca agttcttctt ccgggatctg
    16921 ctgtatcatt ttctcaatga acaagatacc gtctaagtga tcgatttcat gctggaaggc
    16981 acgggctaaa tagccaaaag ccgtcacttc tatctcttct ccgtctcgat cataataacg
    17041 cacggtcact tcatctgctc gcttgaccgt tccatagaca tgcggaatac tgagacaacc
    17101 ttctacgtca aggctttcac cttttgcttc gatgatttct gggttaatca gttcaaattt
    17161 ctcgccttca tctacttcga tcacagcaat tctcttgttt tgtcctactt gaggagcagc
    17221 gatccctatt ccgtcattcg caatcattgt ctcatataaa ttgtctaata aagcgattgt
    17281 ctcgtccgtg attacatcga ttggctgagc agttcttttt aatttatcat tagggtggat
    17341 caatatagga tagcgcataa tttggccctt tcttaaataa agtttaatgg ttcgttgtca
    17401 attgccacaa aaagaccttg tcgctgttct ttctggcttc cattcaaaat ttcatccaaa
    17461 acatgaggaa gattcggttc atgtttatat ttgatgatta gttgataata atatcggttt
    17521 ttcactctca aaatcatgct gggcgttggc cctaatagca agctttgagg gcttaacact
    17581 tcttttagct gattggcaat ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg
    17641 ctggcggtta ttttcacagt aaagtaataa ggaggatagc ctccacgatg ccgtacatgc
    17701 atttcctgct gataaaactg ctcataattt tgttttttag ccaattctat ggcgtaatgt
    17761 tgaggattga atgtttggat cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct
    17821 gaaacttggg taagcaattg gaatgtatgc tcactagaac gaaaatccgg taaattcagg
    17881 gaggtatcag cattcaacac cccaactaat gtcacttctg gaaagtccaa acctttagca
    17941 atcatttgtg tccctaataa tatatctgct tcttttgcac cgaatttttg taaaatctgt
    18001 tcgtgtgccc cttttcttcg agtggtatct acatccatac gcaaaatcct agcttgcgga
    18061 taaagttctc ggagttcctc ttctactttt tgagtacctg ttccatagta gcggatttta
    18121 ttgccgccac aattaggaca gcgattggga ataggttctt catgtccgca ataatggcag
    18181 cgcattgaac gtgtatccat atgcagtgta agagagatat cgcaattagg acatggaaga
    18241 acaaatccac agtccctaca catcacaaaa gacgaatatc ctcgacgatt gagcagtagg
    18301 acagtttgtt cttttttgtc taatcgattt tgtatttttt cctgcaaatt agcagaaaaa
    18361 gtagaggtcc gatgattctg aaactcttca cgcatatcta cgatctcaat agctggaagc
    18421 tgtgcagctg ctttggctcg gtggtttaac tgcaataact gataaacgcc tttttgagct
    18481 cgtgctcttg attctaaaga gggcgtcgcg cttcctagaa ctattgggca atgatgatat
    18541 ttactgcgcc aaattgctag atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa
    18601 cttgattcat gttcttcatc gataatgatc agcccgatat ttttcagcgg ggcaaaaata
    18661 gcagatcgtg cgcctactac tacttgcgct tcttcccgct caatcttgcg ccattcatca
    18721 tatttttcac cttgggaaag tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct
    18781 ttgaatcgct cgaccatttg cggagtcaat gcgatttcag gcactaacat aatagccgtt
    18841 ttattttctg acaaaacatc agcgattgcc tgaagatata cttctgtttt cccgctgcca
    18901 gtgattccct ccagcaaaaa gacctgatct ttttgctgct gacctgctgt gatgatttgt
    18961 tcgacagcat ttttttgttc tgcattcagt tccaatgccg tcgtttggtc aaatactcgg
    19021 tctttataag ggtcgcgata acgttctgat tctataaact ccagccagcc cttttctttt
    19081 ccttgattca agacagcagt actgattcct ttatttttga aataagcaat tggttcttct
    19141 tggctcagtt cagataaaca caaaagaagt tctttttgtt tcttggcatt ttgccgtaat
    19201 tttgcccatt cttcttcgat ttgcttttca gttaacaaag aacggaccaa acgaatcgtt
    19261 tttactttat ttcgtgtatg gacttcatat ttgatatcta ctaattgctc ttggcgccag
    19321 cgaatcaatt gagaaaggct gccttcttct tctgcttcct tccaggaaat cacttctcgc
    19381 tgatcaaaca atacctgaac ttctggtgtg tctgccaaag gatagatcaa tttatcatat
    19441 tctgctttca tcacactcgg aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta
    19501 atttttttca tataatcagc taatgaaagt aattcttcat tgaccaccgg agcaaggtct
    19561 agcaaacgaa taactgcctt caaagatgga ttagctgatt cttctgattt ttggatagcc
    19621 ataacaaatc cttggacatg acgattgcca ttaccaaaag ggacttctac tcgcatcccg
    19681 acttgcaaag ctgtttccat ttcagaggga accaaataag tgaatggctg atcggtctgc
    19741 atagtaggta cgtccacaat gacttcagct gttaatatca tggttcgttt cctccttttt
    19801 ttgtcttttc ttatcaagcg acaaaaagtt ccttgcctat tttactagag atcgatggat
    19861 ttgtcttatc ttctgccaaa tcctaacaaa aaattaacag cagataagaa aaaaaagaac
    19921 tgtgtcaaaa agttcagaca cagccttttc taccagacaa taagtggcga atgtttgaaa
    19981 aggaaacagc tgggtttgct tttcttttta gatgttcgct ctatttttct ttattacatt
    20041 ttttgctctt cacggatacg tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga
    20101 cgttcctctt cttccatctt caaacgttca cgttttaatt caggatgtgg atcattgact
    20161 acatttcctg catcgatttc ttctaaagct tgaccgacgc tttttactga atcaaaagaa
    20221 tctagagtag gctgtgcact tgcatccaat tcatgcgcgc gtttacttgc taaaatcact
    20281 agagaatatt ttgaatttac acgatctaat agtgagtcaa ttgaaggttt taacatcata
    20341 agactacatc tcctttaaca ttttaatata tttaccgatt acgcgatcta ctcgaaaatg
    20401 ttcactggca ataatgtttt tgattcgttc aactgctagc ggcacttcat catttacaac
    20461 agcatagtcg taaagagcca tcatttcgat ttcttctttt gcaactttca ttcgttcttc
    20521 gattacttca tctgcatctg ttcctcggcc tacgatccgt gattttaatt ccgctaaatc
    20581 tggcggagtc aaaaagatga atacaccatc gggcactttt tctttgactt gttttgctcc
    20641 ttggacttca atctccaaga aaacatcttt tccttcatcc aatgtcttgt tgacatatga
    20701 caaaggagtt ccataataat tcccgacata ttctgcgtat tccagcatct ctccagcttc
    20761 gatcattgat tcaaactctt ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc
    20821 ttcacgcatt ttacgggtag tcattgagat cgaatactga aaatcattat cctcactctc
    20881 aaaaattgct tttcgtactg tgccttttcc tacaccggat ggaccagaca acacgattaa
    20941 taatccacgc tctgacatga cgacgtcctt tcaactagtc tttttgtact tatcgtttat
    21001 tttgcacttt ttttctgcaa ctttcaaggt tatctttaat tatagctaga aattggtaaa
    21061 aaagctaccc tgtaccaaga ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag
    21121 ccaaacataa acagcttctc atgtttttcg tttggttggg gttattatcg cagatgcaag
    21181 cttttgaacg ccgtgtataa gaggtcgtga agaagccgat tagtttcgag tatgaagaaa
    21241 aattttgtga aaatagtttc tcacattttt gcataaattt ggcttaatac cgagaaaatg
    21301 gtttttgaac atcacttatt caaaataaaa aaagattgtg acacgaattt tgtcacaacc
    21361 tttaaatgta ttcctagagc aaagaagcta agcgaagaaa tccaattctg attatttatt
    21421 gtatttcttt gttcagcatc cttgctaaaa gcccatattg gacaaagtat tcactacacc
    21481 catgatctca tcttttcgag aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc
    21541 aattcgaaca ataaatttcc gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa
    21601 cagttcgttc tcattatagt ctgcacaggc atctttccat acgtcaaagc cgtttttttc
    21661 agaagggata tcccgtttaa ccacagtttt tccacttaat tcgtggaacg ttacgtcata
    21721 atatttcatt atctctacct ctttcgtcat tcaatatttt gtacttgttc tctgattttt
    21781 tctaagatcg tcttagactg gacgacttga tttttaattt cgattgcatt tgatttggaa
    21841 ccgatcgtgt tgatttcccg attcatttct tggatcagga aatctaattc ccgacctaca
    21901 ggacattgtg cttcgataag ttcatctagt ttttgtacat ggatcgccag acgatccagt
    21961 tcctcgtgaa tatctccgcg ttccaacaag atggctagct ccgtcaacaa ccgttgctca
    22021 tcgactttcg ctccaagaaa ttcctccagc tttttttgat accttatttg aaattccttt
    22081 tcatagattt ctacaaaact atcgagtttt tttagtactt ctttgaattc ttcagtattc
    22141 tttttcaaca ctaaagctaa agcttgacct tctttttctc gactcgcctc aatagaatca
    22201 agtgcgcttt tcaatgtatc tagagctaac tgtgccaatt cttccatgct ctcttccggt
    22261 ttttcttcta ccgttacgaa gttttcgtcc agcgcgaagc gttccgccat ccgactaatt
    22321 gacacttgat tttgactacc gtatcgttgt tgaattccgt ctgtcaattg agtgataagt
    22381 gaatcaatca attcccagtt gatgacgatc tgtttttcgt ttttgccaat ataagctaga
    22441 ttcacaaata cttcgattcg accgcggctt aactgctgtt taataagctg acgaaaatcg
    22501 ttttcaacaa agttcaggat tttaggactt cgtatttgaa catctagaaa acgctggttg
    22561 acacttttta tttcaatatc taactgatac gcttttgttt cgcggatggc tttgccaaaa
    22621 ccagtcatac ttttcattcg acagggtcct tcctcattac atattttcta ttgctttttg
    22681 cagttgttca aactcttcgt tcgtcaatgt caggcctttc cccatttttt catggtctgg
    22741 cgcccaatct cgaagatcga attttggcgg gcgaccattc cagctgacta ggttcagttc
    22801 tttacgccac cccttgttgt tttctgacaa tatagcgatt tcttctacga tttcatatga
    22861 aaattcttgt gccatggttg ttcctccttg agataatata aatataggat cagtaatgtg
    22921 tcaactggtt gcttaagcaa ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga
    22981 taatcaaaat aaacagtttt cgtgcgctca ttttgtattt tttgctggta ttttcgatac
    23041 cttcgccatt cttcagtact taattgacca atttgtgcca ataaatcatc cggcatactt
    23101 tttatatctt gttggcagat catttgaatc atcatgtcta cttgatagat ttcattgggc
    23161 agtaaaaaat gtttttttgc taaaagttct agcatcgcca atccgcatgt ctctggtacg
    23221 accctgttct tatataaatt acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat
    23281 aattctaact gaatttcttt tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca
    23341 ggtttaacag aatgaaatgt ataccagttc ccatagtaat agcctaatcg ttttttcatt
    23401 tccagatagc ctaattgtaa atttaattgg ttcctttggc tatcaaaaag cagaaagctg
    23461 cctagtgtcc acaatgtttg acatttccaa tggataaaaa tgtctggcat acgaattttt
    23521 ttcgctgggc ctggcccttg cacatctacg acaaatgcct ctgttgctcc tttgttaatc
    23581 gcaatatcaa taggtatttt gttgcgatac ccaccgtctg catatttatt ttttccgatc
    23641 tttcgataag ccatagcggg ataaaatgaa gcagaggcga gtatccaatc tggtatttct
    23701 tctgtttttg tctgttggat atcagtcact acttccgcta gttctggcag tttagtagac
    23761 accgtatata aacgtatttt gggattatgt ttgagtaagg acaaatccaa attgtctttg
    23821 accatctttt ccaatggagc agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt
    23881 tggcgtcttt ctcgatagaa gcgttcacga aggtcttcaa ctgcagccat ttctggtaac
    23941 gcaagcactt gtcttgtcga cagctttttc cataaattca cagctttttt catatctcct
    24001 tgtaaaatca atgcgccgtt caacgcacct accgaggtac ctgtgataat ctcaaagttg
    24061 atatcatgtt ccttcagcgc ctgccacaca ccaatctgat aagcaccgtg tgctcctcct
    24121 ccacctaaaa caagtgctgt atgatagacc agctttttat ttaattgtgt ggttccttga
    24181 tagccgtttt tatgaaaaat acgcaaccat tcttctgata gaggagattg aaataaaaaa
    24241 caacagtttt ctttaataaa aaaacgagcg caagcttcca tataccgaaa aaattttttc
    24301 cattcaaaag tttcaccttc aatcaacaga ttattaaaaa taacatctcc tctactttta
    24361 gcaatagtaa aatagacggt atattttttc gatactgctt taaaagcaca ttgctttttt
    24421 gcagaacgcc aaaaagcgtc tctactttct tgatgacttt gaaaaaatgg aaggggttgt
    24481 tttgcttgct ggataggcga atacagcgac attttctgtg aaaagtaaac tttttttatc
    24541 tccatcgcgc tctccttttt cttcaagtat accagtacgt cataaaaagg tcatga
//
LOCUS       DAXWLB010000022        24579 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXWLB010000022 DAXWLB010000000
VERSION     DAXWLB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.022"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(153..551)
                     /locus_tag="ACGE4H_000713"
     CDS             complement(153..551)
                     /locus_tag="ACGE4H_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFF8364152.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACGE4H_000714"
     CDS             complement(548..1321)
                     /locus_tag="ACGE4H_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8364153.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACGE4H_000715"
     CDS             complement(1318..2001)
                     /locus_tag="ACGE4H_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFF8364154.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACGE4H_000716"
     CDS             complement(2168..3055)
                     /locus_tag="ACGE4H_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364155.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACGE4H_000717"
     CDS             complement(3052..3921)
                     /locus_tag="ACGE4H_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364156.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACGE4H_000718"
     CDS             complement(4058..5344)
                     /locus_tag="ACGE4H_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364157.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACGE4H_000719"
     CDS             5617..6195
                     /locus_tag="ACGE4H_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8364158.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACGE4H_000720"
     CDS             6195..7124
                     /locus_tag="ACGE4H_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364159.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACGE4H_000721"
     CDS             7121..8857
                     /locus_tag="ACGE4H_000721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8364160.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4H_000722"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACGE4H_000722"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFF8364161.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACGE4H_000723"
     CDS             complement(9753..10262)
                     /locus_tag="ACGE4H_000723"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFF8364162.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4H_000724"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACGE4H_000724"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFF8364163.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACGE4H_000725"
     CDS             complement(11563..12654)
                     /locus_tag="ACGE4H_000725"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFF8364164.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4H_000726"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACGE4H_000726"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFF8364165.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4H_000727"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACGE4H_000727"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFF8364166.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4H_000728"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACGE4H_000728"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFF8364167.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACGE4H_000729"
     CDS             complement(16617..17591)
                     /locus_tag="ACGE4H_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFF8364168.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACGE4H_000730"
     CDS             complement(17572..19455)
                     /locus_tag="ACGE4H_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFF8364169.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACGE4H_000731"
     CDS             complement(19473..21698)
                     /locus_tag="ACGE4H_000731"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8364170.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACGE4H_000732"
     CDS             21813..22847
                     /locus_tag="ACGE4H_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8364171.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACGE4H_000733"
     CDS             complement(22914..23927)
                     /locus_tag="ACGE4H_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8364172.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACGE4H_000734"
     CDS             complement(24005..24193)
                     /locus_tag="ACGE4H_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFF8364173.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7065 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaaa
//
LOCUS       DAXWLB010000023        24335 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXWLB010000023 DAXWLB010000000
VERSION     DAXWLB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.023"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     misc_binding    60..265
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            377..1633
                     /gene="tyrS"
                     /locus_tag="ACGE4H_000735"
     CDS             377..1633
                     /gene="tyrS"
                     /locus_tag="ACGE4H_000735"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFF8364174.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            1924..3801
                     /gene="tdc"
                     /locus_tag="ACGE4H_000736"
     CDS             1924..3801
                     /gene="tdc"
                     /locus_tag="ACGE4H_000736"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFF8364175.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4044..5465
                     /gene="tyrP"
                     /locus_tag="ACGE4H_000737"
     CDS             4044..5465
                     /gene="tyrP"
                     /locus_tag="ACGE4H_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFF8364176.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5574..6941
                     /gene="nhaC"
                     /locus_tag="ACGE4H_000738"
     CDS             5574..6941
                     /gene="nhaC"
                     /locus_tag="ACGE4H_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8364177.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7222..8241)
                     /locus_tag="ACGE4H_000739"
     CDS             complement(7222..8241)
                     /locus_tag="ACGE4H_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8364178.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8263..9270)
                     /locus_tag="ACGE4H_000740"
     CDS             complement(8263..9270)
                     /locus_tag="ACGE4H_000740"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8364179.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9263..9928)
                     /gene="pgmB"
                     /locus_tag="ACGE4H_000741"
     CDS             complement(9263..9928)
                     /gene="pgmB"
                     /locus_tag="ACGE4H_000741"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8364180.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(9921..12215)
                     /locus_tag="ACGE4H_000742"
     CDS             complement(9921..12215)
                     /locus_tag="ACGE4H_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFF8364181.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12475..14640
                     /locus_tag="ACGE4H_000743"
     CDS             12475..14640
                     /locus_tag="ACGE4H_000743"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFF8364182.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            14944..15777
                     /locus_tag="ACGE4H_000744"
     CDS             14944..15777
                     /locus_tag="ACGE4H_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFF8364183.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(15879..17579)
                     /locus_tag="ACGE4H_000745"
     CDS             complement(15879..17579)
                     /locus_tag="ACGE4H_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364184.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            18086..19246
                     /locus_tag="ACGE4H_000746"
     CDS             18086..19246
                     /locus_tag="ACGE4H_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8364185.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19262..19705
                     /locus_tag="ACGE4H_000747"
     CDS             19262..19705
                     /locus_tag="ACGE4H_000747"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364186.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20188..20718)
                     /locus_tag="ACGE4H_000748"
     CDS             complement(20188..20718)
                     /locus_tag="ACGE4H_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFF8364187.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            20823..21374
                     /locus_tag="ACGE4H_000749"
     CDS             20823..21374
                     /locus_tag="ACGE4H_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8364188.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21527..22000)
                     /locus_tag="ACGE4H_000750"
     CDS             complement(21527..22000)
                     /locus_tag="ACGE4H_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8364189.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22138..23610
                     /locus_tag="ACGE4H_000751"
     CDS             22138..23610
                     /locus_tag="ACGE4H_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8364190.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23607..24317
                     /locus_tag="ACGE4H_000752"
     CDS             23607..24317
                     /locus_tag="ACGE4H_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFF8364191.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         7555 a         4389 c         5047 g         7344 t
ORIGIN      
        1 tatccttttt ctgttgacaa agaaaaagtt gcgctctatg atagacacat attaataata
       61 acaagacgaa gatcgaaacg aagtagttgt taaagccaat gtttcagaaa gctggtggtc
      121 gctgcaaacc agtacatgct ttaaagcgaa ttacatttca ggagtctttg gcagtgattg
      181 ccacgcgaca gcgttaaatg ttcgagaggt atgttggata tctagacata caaattgggt
      241 ggtaccgcga gatttcgtcc cttggcttat atgccaaggg acttttttta tttcctatcc
      301 tatcctgaag aaataattaa ttacacatgc tagcaggttc ctgctttcat aaaaaaacta
      361 cacggaggtc agtaaaatga atattattga tgaattaact tggcgcgacg ccattaatca
      421 gcaaacaaac gaagaaagac ttcgcgagct cgttgaagaa aagagcattt cactttattg
      481 cggcgttgat ccaactggtg attcgatgca tatcggacat ttgattccat ttatgatgat
      541 gaaaagattc caattggcag gccatcatcc atatattctg attggcggcg gaacaggaac
      601 gatcggcgac ccaagcggcc gcaaaacaga acgtgtgttg caaacgatgg aacaagtgca
      661 acataacgtg gacgcacttt ccaatcaaat gagaaaattg tttggtaaag atgcaaatat
      721 cacatttgtg aacaactacg actggttgtc aaaaatctct ttacttgaat ttttgagaga
      781 ctacggtaaa aacttcaaca tcaacacgat gttagcaaaa gatatcgttg ccagccgttt
      841 ggaagtagga atctcattta cagagtttac ttaccaaatc ctgcaatcta tcgacttttt
      901 gcatctgcat aaaacatacg atgtgcaatt gcaaatcggt ggagcagacc aatggggaaa
      961 tatcacagct ggattagacc taatccgaaa attagaagga ccagaagcag aagcgttcgg
     1021 tttaacgatc ccattgatgc tgaaggctga cggaacgaaa ttcggaaaaa ctgcaggcgg
     1081 tgccgtttgg cttgatccga agaaaacttc accatttgag ttctaccaat tctggttgaa
     1141 tcaagatgat cgcgatgtag tgaaatactt gaaattcttt actttcttgt ctcaagaaga
     1201 aatcgaagac ttagcgaaaa aagtagagac agaacctgag aaacgtgaag cacaacgccg
     1261 tttggcagaa gaagttacaa gatttgtgca tagtgaagaa gacttgaaag aagcgcaaaa
     1321 aataacgcaa gcattgttct ctggaaatat taaagaacta aatgcggaag aaatcgcgca
     1381 aggattcggt aaaatgccga acgttgaaat ttcaagcaca cctgaaaata tcgtggaact
     1441 gcttgtttcc acaaaaattg aaccatctaa acgtcaagca cgtgaagatg tttcaaacgg
     1501 agctataagt attaacggtg accgcgttac cgatttaaat tttgtcataa atccatccga
     1561 tgaattcgat ggtaagtttg tggttattcg aaaaggtaag aaaaattact ttttggccaa
     1621 agtaattgat tagaatttag taagttttta ccttgaaaaa gggaaatact attacaacat
     1681 tccaattttg aaacccatcc taaaatagaa cgctggttgt cgttaataca atctttgcga
     1741 gggagtttac atttgtaaat tccctcgttt tttttaatta tttcaaaagt gtgaacaatt
     1801 cggataattg atacatatta aaatatgatt aaaaatcagg ccaaaactta gtttacagaa
     1861 tagaagaaca ggtgtaatgt agggctcgac aaaatttaaa tattttttta ggagggtttc
     1921 actatgagtg aatcattgtc gaaagatcta aacctaaatg ccctattcat tggggacaaa
     1981 gctgagaacg gccaaatcta taaagctttg ttgaatgaat tggtggatga acatttaggc
     2041 tggcgtcaaa actacatgcc tcaagacatg cctattatta cgccagaaga aaaaagcagt
     2101 gctagttttg aacacactgt gaataaaaca aaggatgttc tatcagaaat ttcagcgcgg
     2161 atgcgtaccc attccgttcc ttggcataat gccggacgtt actggggtca tatgaactct
     2221 gaaacattga tgccatcttt actagcgtac aattttgcta tgctatggaa tggtaataac
     2281 gttgcctatg aatcttctcc tgcaacaagc caaatggaag aagaagtagg aatggaattt
     2341 gcaaaattaa tgagctataa agatggctgg ggccacatcg ttgctgatgg ttctttagca
     2401 aacttagaag gactttggta tgcccgcaac atcaaatcat tgccgcttgc aatgaaagaa
     2461 gtgacacctg aactagttgc tggaaaaagc gattgggaat taatgaactt gtcaacagaa
     2521 gaaatcatga atctgttaga cagcgtccca gaaaaaatcg acgaaatcaa agctcactca
     2581 gcacgtagcg gaaaacattt agaaaaatta ggaaaatggt tggttccaca aactaaacac
     2641 tattcatggc taaaagctgc cgacatcatc ggtatcggtt tagaccaagt gattccagtg
     2701 ccagttgacc acaactatcg catggacatc aacgaacttg aaaaaatcgt tcgtggtcta
     2761 gcagctgaaa aaacaccaat cctaggtgta gtcggtgttg taggatcaac agaagaagga
     2821 gcaatcgacg gtatcgataa aatcgttgca ttgcgtcgcg tattagaaaa agacggtatt
     2881 tacttctact tacacgtaga tgctgcttat ggcggatacg gccgtgcgat tttcttagac
     2941 gaagacaaca acttcattcc atttgaagat ttaaaagatg ttcattacaa atacaatgtc
     3001 tttacagaaa acaaagatta tatcttagaa gaagtacaca gtgcatataa agcaatcgaa
     3061 gaagcagaat ctgtaacgat tgacccacat aaaatgggct atgttccata ttctgccggt
     3121 ggtatcgtca tcaaagatat ccgcatgaga gacgttatct cttactttgc gacatatgta
     3181 tttgaaaaag gcgcagatat cccagcatta cttggggctt atatcttgga aggttcaaaa
     3241 gcaggggcaa cagcagccag cgtttgggca gcacaccatg tcttaccttt gaatgtcaca
     3301 ggatatggta aattaatggg tgcatcaatc gaaggtgcac atcgtttcta caacttcttg
     3361 aatgacttgt catttaaagt aggagacaaa gaaatcgaag ttcatccatt aacttatcca
     3421 gatttcaata tggtagacta tgtattcaaa gaaaaaggca acgatgactt agttgcaatg
     3481 aataaattga accatgacgt atatgattat tcttcatacg tgaaaggaag catttacggt
     3541 aatgagttct taacttcaca tactgacttt gctatcccag actatggcaa cagcccatta
     3601 caatttgtga accaactagg attctcagat gaagaatgga accgtgccgg aaaagtaact
     3661 gtattgcgtg ccagcgtaat gacaccatac atgaacaaag aagaacactt tgaagaatat
     3721 gctgaaaaaa tcaaagcagc tcttcaagaa aaattggaaa aaatctacgc agaccaatta
     3781 ttggcaagcg aagcaaaata agcaaggtaa tcatgttaga aaacagcttg aatactaact
     3841 gtttctgacg catgtttgga tctgaagagc aaggggagac ctaagctctt cgatcccttt
     3901 gttttgaggt tgtctttgaa gaaacccttc aagcaataaa ctcaaagaat cccctcaaat
     3961 attcgctgaa ttgtttcgtt tattgctgaa actacttcaa agacaccgtt attttatcca
     4021 aaaaatcaag gagagtgtca acggtgagta aatctgaagt aagtaaaatg ggtcttggga
     4081 cctttatcgg tctgacaatg gcactgtgtg caacggttcg gagtattcct acgttagccg
     4141 cagtcggttg gacattgatt ttttattcaa tttttgcagt tttgtttttt gccggtccga
     4201 tatcaatggt ttccggtgag ctttccacga tgcttcctca agaaggcggt cctcagttat
     4261 gggtaaaaac agcactgggc agcaaatggg gatttgtcgt tgcatggcta ttatgggtgc
     4321 aaatgttccc aggtatggta atggtcgctt ctactttagg accattgtta ggaaacacat
     4381 ttggtaatgt agaacttggc aataatcact ggttcgtttt aggttgtatt ttagttatct
     4441 actggatcat cacgatcttg aacttgaaat tcgatatggc aaaagtcggc gggaacatcg
     4501 gtgtatggct aggggtatat atcccagtag tcatcatgtt cgttttaggt gttttggcag
     4561 ccttcaaagt cggactagtc tcaaacggct atctaggtga cttttcatgg tcaaaagctt
     4621 tccctgactt ggaacatatt gattcattga aatacttagc tggtatcaca tttatttttg
     4681 ttgggatcga aatgagttct gtttacatgc ctcgtttgaa agacgcaaca aaaaattata
     4741 caaaaggcgt atttatcgct ttgatcggat tagttttatt gaatgtcatc aatgcgatgc
     4801 ttgtagcaaa tgtcgtacca gatggaaaaa tggaattagc aaatatcacg cagcctatct
     4861 tgattgactg tcaaatccta ggcttaccag aagtgatcgg aaatatcttc agcttcatgg
     4921 tatttattgg tgtgctttta caattatcag cttgggttac tggaccatca aaaacaatca
     4981 tccaagtagc tcgtgaagga ttccttccac caaaattcgg attccataaa gaaaacaaat
     5041 acggcgtatc tagaaatgtc gttctaacac aatcaatcgt tatttcatta tttgctttgc
     5101 tttatggtgt aatggatgac gtaagtgctg tattcttgac acttacaaat gctacaactg
     5161 ttatttattg tatcgtgtac attttgattg ctgtttcatt gcttaaaatg cgtaagaaac
     5221 atccagaatt cgaacgtcct taccgaattg gtaaaaacgg aaacggatta gcttgggtag
     5281 tcagctgcat gctgatcttc tctatcatcg ttgtcgtatt tgctacatta ggtacagcga
     5341 cattgagtga tgcattatta gtagcagcta tcacagtggt catgttcgtt attccattga
     5401 tcatcaatca cttcaagaaa gattcttggg ggattgaggt agaaaaaagt ttagaagaaa
     5461 aataaaaaaa taagtctttc agccgacgtt ttgtcgctga tttagtagga ggtcctgcca
     5521 agtgaatagt tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata
     5581 aaaatatttc tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag
     5641 tactgatcat cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt
     5701 tgttgtttta tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca
     5761 aaggaatcac tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg
     5821 cttcatggat tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt
     5881 ctgtgcgttt ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg
     5941 gcagttcatt tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac
     6001 ttggattcaa tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata
     6061 actgctcgcc tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat
     6121 tcgaacatat cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt
     6181 tttatacgat attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga
     6241 tccaaaacat gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt
     6301 tatttggggg ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag
     6361 ctgctctcgt tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca
     6421 tcttgatgaa tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc
     6481 gaggcgggat catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag
     6541 gtgggttact gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga
     6601 tggaccgacc ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga
     6661 tcgttggaga acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg
     6721 atgacgttgg cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg
     6781 tcaattctct cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta
     6841 gcgcgttgca ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat
     6901 taggcggttt tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct
     6961 gggacagaag cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat
     7021 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
     7081 ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac
     7141 aatgtgttag aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag
     7201 cgtatttagt gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt
     7261 gatcaagcga tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc
     7321 gcgcagcttt tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag
     7381 gtaagggtgt gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc
     7441 tggaatgaca tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc
     7501 aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc
     7561 ctccggatgc tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt
     7621 ttgataaccg aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag
     7681 gatattctta tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt
     7741 atcattatct acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa
     7801 agggatgtgg ttttggtata aataatcaat gacagtatct tgactatctg aatacactag
     7861 aataaaacca tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc
     7921 aaaattttta gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat
     7981 tgcttccatg atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg
     8041 taagattacg ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg
     8101 aacataacct agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact
     8161 aggatgatcg ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac
     8221 atcttttacg gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg
     8281 ataaatcgtc tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg
     8341 gaaatgaacg atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat
     8401 ctgttttcca ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact
     8461 tttattagaa gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga
     8521 agttttggaa agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca
     8581 tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt
     8641 tgtccaattt ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac
     8701 cgttaactga tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg
     8761 gttggtcgga ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc
     8821 gttttttgtt aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc
     8881 atcttttaag taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca
     8941 aaattggaaa gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc
     9001 caatctatgc tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc
     9061 tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt
     9121 ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag
     9181 aagaacgact tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac
     9241 tggtacgacg gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga
     9301 tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc
     9361 ccacgccaat tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt
     9421 cgatcccgat acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat
     9481 ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg
     9541 taagacccat tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct
     9601 tgattttttc ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg
     9661 gttcgatcgt ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa
     9721 attcttcctt tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt
     9781 cttctcggct gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt
     9841 cctgaccaag ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt
     9901 ctaaatcaaa caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga
     9961 cgatccgcta aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat
    10021 aactcgaaag taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg
    10081 atatggaaag aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca
    10141 tgggcagttt tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa
    10201 gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt
    10261 cgctggtaca tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg
    10321 atacttggcg aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt
    10381 tgcttttctt cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc
    10441 tgcttgatga agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga
    10501 tcgcttgtgg gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg
    10561 acaaagattc ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat
    10621 ttggctagtt cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt
    10681 tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata
    10741 ttgttctcat attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt
    10801 gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca
    10861 tttttcaaat attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc
    10921 gcgccatttc ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca
    10981 gtgaaggtga ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt
    11041 gcttgcggca gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct
    11101 aaagcaagat aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat
    11161 ttttctcctg tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta
    11221 gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg
    11281 accacatcgg caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg
    11341 atataattgt ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct
    11401 ggaatatctc tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga
    11461 gataattctg cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg
    11521 actgattttc cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca
    11581 aagttgtttg gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca
    11641 atctcattcc aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat
    11701 tttgatacga gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa
    11761 gctgattctt ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta
    11821 gaagtagtga ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa
    11881 aagtcttctg gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc
    11941 gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac
    12001 cagccgacac gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg
    12061 tcccctgagt aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt
    12121 gttaaagatt cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat
    12181 ggatcaatag caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt
    12241 atacgttcat aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat
    12301 agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt
    12361 ataggtttta tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa
    12421 aacgcacgct ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa
    12481 aagatattta gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca
    12541 gttatgccgg cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct
    12601 gattggacgc cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt
    12661 ggtaatcttc atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca
    12721 ggcggagcat ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc
    12781 tttggtgtca caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt
    12841 acgaagatca tggttaaagg attttttaca agtgtattgg aagccccagc tttgaatatg
    12901 ggagtattcg tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat
    12961 aattatagaa aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt
    13021 gttgttatct tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta
    13081 caggctggaa tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt
    13141 ctagcaccgt ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat
    13201 atgctgacga ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc
    13261 gcacaggcgg ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat
    13321 ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg
    13381 acgccggctc gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg
    13441 gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat
    13501 ttttcagcag cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt
    13561 atgtttgctg ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg
    13621 atggcagata tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg
    13681 acaccgttag ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg
    13741 atcgcttttg gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt
    13801 gctacaccgg gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac
    13861 agcggaacag gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa
    13921 gaaaatatca aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga
    13981 gaaaaagtag ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa
    14041 gacaatggag tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa
    14101 gatctgctgc aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga
    14161 caagcaggta gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga
    14221 gaagtgattc ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac
    14281 ggctttgctg ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca
    14341 agtattttcc ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg
    14401 atccatatgg gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa
    14461 gaaggccaag aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa
    14521 aacgaagaaa aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta
    14581 gtgatcaagc aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa
    14641 ggagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc
    14701 tcctcctatt tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt
    14761 agtggagagg tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa
    14821 tagctcctgg tccattgagc ttattactga ggagttagca ggagaacact atttgctgga
    14881 gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa
    14941 ccaatgaaat tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa
    15001 ttgcaagaat tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa
    15061 atcaaccaat tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg
    15121 gtaaagaatc aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta
    15181 aaggaacacg gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa
    15241 tatgaagaag gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc
    15301 tcccaatttc aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag
    15361 atagatgatc aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa
    15421 agtggctttt attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca
    15481 ctttttttcc taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga
    15541 gaaagttgcc ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct
    15601 actgtggaaa agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt
    15661 attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat
    15721 agtccaatca tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa
    15781 atataacata taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt
    15841 tggagagttt tttgatcgga tgtatttatc ccttttgcct aagcatgtaa aagcttgtat
    15901 ctatgataaa taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg
    15961 aaatagaaat agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc
    16021 ttcacgattg gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc
    16081 tgtagcacag tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt
    16141 ttccacttga ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct
    16201 agtatttctt cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga
    16261 tagtcaggtg tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat
    16321 gttttccttg cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca
    16381 ttttcccact tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga
    16441 atgccaaata tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg
    16501 gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc
    16561 aaactaataa aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa
    16621 agaaaaaaga aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt
    16681 tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa
    16741 tggagccaat aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag
    16801 ggaggaagaa tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc
    16861 agaacaaaat aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc
    16921 tggttaaaaa agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac
    16981 agtaaatgga tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt
    17041 ttatgaagga cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag
    17101 ctagtgaatc cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg
    17161 gctactggta cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa
    17221 aatcctgaaa taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct
    17281 gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg
    17341 atcctgccac tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta
    17401 atttgttgcg cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt
    17461 agtttatgtg tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa
    17521 aatgggccat attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata
    17581 tatcctatgc gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta
    17641 caagaaaaac ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc
    17701 tccttgtttt gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct
    17761 ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata
    17821 tgagaagcta ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac
    17881 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa
    17941 aatatgagta gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac
    18001 aacctcatcg gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca
    18061 aaaaaacttg caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact
    18121 tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc
    18181 attttatgtg gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa
    18241 acggaccgca gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt
    18301 aactgaccaa ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag
    18361 tacaacatcc agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca
    18421 attaaatatt tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt
    18481 cttgcaagga acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca
    18541 cagcggttta cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac
    18601 gtttattttg actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt
    18661 actgccagat gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct
    18721 tacatgtacg ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc
    18781 ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca
    18841 atggtttatt cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg
    18901 actaatctat cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga
    18961 ttcgtcaggg aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact
    19021 tactagaaat ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga
    19081 tcaattgcct ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc
    19141 ttttggtatt agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt
    19201 aaaagagctt caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa
    19261 aatgatccgc acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg
    19321 gctgcatgct aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt
    19381 agaatctgtt aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga
    19441 aattattggt tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga
    19501 ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac
    19561 actgactttg acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg
    19621 ttttacaatt caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa
    19681 atggaatcaa aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat
    19741 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
    19801 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    19861 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
    19921 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
    19981 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
    20041 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    20101 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
    20161 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
    20221 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
    20281 attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt
    20341 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
    20401 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
    20461 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
    20521 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
    20581 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
    20641 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
    20701 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
    20761 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
    20821 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
    20881 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
    20941 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
    21001 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
    21061 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
    21121 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
    21181 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa
    21241 cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc
    21301 atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta
    21361 accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa
    21421 acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt
    21481 agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta
    21541 acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg
    21601 tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta
    21661 aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac
    21721 ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa
    21781 atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt
    21841 ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc
    21901 ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt
    21961 gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc
    22021 tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg
    22081 aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg
    22141 agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt
    22201 gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt
    22261 cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt
    22321 ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt
    22381 tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt
    22441 acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga
    22501 gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga
    22561 tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta
    22621 tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc
    22681 gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat
    22741 cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag tttactttac
    22801 ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt
    22861 gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc
    22921 ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta
    22981 acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt
    23041 ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct
    23101 atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca
    23161 gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca
    23221 gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg
    23281 ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg
    23341 gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg
    23401 ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta
    23461 gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta
    23521 gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct
    23581 gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg
    23641 cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt
    23701 tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg
    23761 gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag
    23821 gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc
    23881 gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag
    23941 tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa
    24001 acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag
    24061 tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt
    24121 tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc
    24181 aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac
    24241 aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag
    24301 aagcaaaagg cgaataatat tttgaacacc atgta
//
LOCUS       DAXWLB010000024        24312 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXWLB010000024 DAXWLB010000000
VERSION     DAXWLB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.024"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE4H_000753"
     CDS             complement(216..1511)
                     /gene="purB"
                     /locus_tag="ACGE4H_000753"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFF8364192.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE4H_000754"
     CDS             complement(1524..2648)
                     /gene="purK"
                     /locus_tag="ACGE4H_000754"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFF8364193.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE4H_000755"
     CDS             complement(2641..3129)
                     /gene="purE"
                     /locus_tag="ACGE4H_000755"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFF8364194.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3545..4852)
                     /locus_tag="ACGE4H_000756"
     CDS             complement(3545..4852)
                     /locus_tag="ACGE4H_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFF8364195.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4855..5436)
                     /locus_tag="ACGE4H_000757"
     CDS             complement(4855..5436)
                     /locus_tag="ACGE4H_000757"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFF8364196.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5552..5649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6008..7300)
                     /locus_tag="ACGE4H_000758"
     CDS             complement(6008..7300)
                     /locus_tag="ACGE4H_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8364197.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7297..7920)
                     /locus_tag="ACGE4H_000759"
     CDS             complement(7297..7920)
                     /locus_tag="ACGE4H_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364198.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7923..8759)
                     /locus_tag="ACGE4H_000760"
     CDS             complement(7923..8759)
                     /locus_tag="ACGE4H_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364199.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8776..9663)
                     /locus_tag="ACGE4H_000761"
     CDS             complement(8776..9663)
                     /locus_tag="ACGE4H_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364200.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9679..10962)
                     /locus_tag="ACGE4H_000762"
     CDS             complement(9679..10962)
                     /locus_tag="ACGE4H_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364201.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRIKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11645..12328)
                     /locus_tag="ACGE4H_000763"
     CDS             complement(11645..12328)
                     /locus_tag="ACGE4H_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8364202.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12346..12963)
                     /locus_tag="ACGE4H_000764"
     CDS             complement(12346..12963)
                     /locus_tag="ACGE4H_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFF8364203.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12973..13797)
                     /locus_tag="ACGE4H_000765"
     CDS             complement(12973..13797)
                     /locus_tag="ACGE4H_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFF8364204.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGTKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13790..14350)
                     /locus_tag="ACGE4H_000766"
     CDS             complement(13790..14350)
                     /locus_tag="ACGE4H_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364205.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14524..15480
                     /locus_tag="ACGE4H_000767"
     CDS             14524..15480
                     /locus_tag="ACGE4H_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8364206.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15785..16741)
                     /locus_tag="ACGE4H_000768"
     CDS             complement(15785..16741)
                     /locus_tag="ACGE4H_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFF8364207.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16845..17447
                     /locus_tag="ACGE4H_000769"
     CDS             16845..17447
                     /locus_tag="ACGE4H_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8364208.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17535..19034)
                     /locus_tag="ACGE4H_000770"
     CDS             complement(17535..19034)
                     /locus_tag="ACGE4H_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFF8364209.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19050..19574)
                     /locus_tag="ACGE4H_000771"
     CDS             complement(19050..19574)
                     /locus_tag="ACGE4H_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFF8364210.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19872..20621
                     /locus_tag="ACGE4H_000772"
     CDS             19872..20621
                     /locus_tag="ACGE4H_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFF8364211.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20612..21397
                     /locus_tag="ACGE4H_000773"
     CDS             20612..21397
                     /locus_tag="ACGE4H_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8364212.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21366..22082
                     /locus_tag="ACGE4H_000774"
     CDS             21366..22082
                     /locus_tag="ACGE4H_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8364213.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22184..23203)
                     /locus_tag="ACGE4H_000775"
     CDS             complement(22184..23203)
                     /locus_tag="ACGE4H_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFF8364214.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23222..24223)
                     /locus_tag="ACGE4H_000776"
     CDS             complement(23222..24223)
                     /locus_tag="ACGE4H_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFF8364215.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7187 a         5252 c         4254 g         7619 t
ORIGIN      
        1 tattcttcat cgatcattaa cagacaaaga actggcccgc caaggatatg aggacatctc
       61 cttgaagtac cagttcgtac actcaactta ttgaaccgcc gtatacgggt ccgtacgtac
      121 ggtggtgtga gaggggcgga aggtcagttg gctttcccct ctactcgatt atttttgcta
      181 ttctatttta attgaatttt gcaactcact cttctttatt ccaaacctac tcgtttgaat
      241 atcacgtcaa cgtttttcaa atgatgatga taatcaaagg catcgtctat ttcttcttta
      301 gataataccg atgtgatggt tggatcttct tctaacagcg ggcggaactg cacttggttg
      361 tcccaagcgt acgcagtttt tggctgaaca agatcataag cttcttctct tgtcattccc
      421 ttatcgatca atttcagcat aacgcgctgg ctataaatca aaccaaatgt tgcagacata
      481 ttccgcttca tgttttcagg aaagaccgtt agatttttaa cgatattgcc aaaacgattt
      541 aacatgtaat tcaataaaat cgtggtatca ggcaagatga ttcgttctgc tgaagaatga
      601 gaaatatctc tttcatgcca taatgtgaca ttttcatagg cagtgaccat gtgtccgcgc
      661 accactctgg ctagaccagc catattttct gaaccgatcg gattcctttt gtgcggcata
      721 gcagaagaac ctttttgacc tttcgcaaaa aattcttcta cttcacgtgt ttcggatttt
      781 tgcaatccgc gtatctctgt tgcaaaacgt tcaatacttg tagcaataag cgccatcgct
      841 gagaaatatt cagcatgtag atcccgaggg agaacttgag tagagatttc ttgtggacgg
      901 atccccaatt gtttacatac atactcttca ataaatggcg ggatatttgc gaacgttccc
      961 actgctcctg agatttttcc tgcttctact ccttttgccg catgttcaaa tcgttcgatg
     1021 ttccgtttca tttctgaata ccatgtggca agtttcaatc caaaagtagt tggttcagca
     1081 tggacaccgt gtgtccgacc catcatcact gtatatttgt gttcttttgc tttttcgcca
     1141 atgatttccg taaaacgttt caagtcttcc cgtaaaatat catttgcttg cttgatgaga
     1201 tagccataag cagtatctac gacatccgta cttgttagac cataatggac ccattttctt
     1261 tcttctccca atgtttcaga aaccgctcgt gtaaacgcaa cgacatcatg gcgtgttaat
     1321 ttttcgattt ccaagatccg atcgatatca aatgatgcat gctcacgaat ttttttcaca
     1381 tcttcttgcg ggatttcacc aagttctgcc caagcttcat ctgctaaaat ctcgacttcg
     1441 agccacgcac ggtatttgtt ctcttccgtc cagatattcc ccatttcagg gcgtgtataa
     1501 cgttctaaca tgttttttct cctctaatcc caaatattcg tttgtttgat ttcttccagc
     1561 gtttgctgga tgtcttctgt caagatagtg atatgtccca tcttgcgacc tttttttgct
     1621 tgtttttttc catagaaatg gaattgccag tctggcttat tctcaatcaa gcgatagctt
     1681 tccagcagtt gttctcccaa aacattgacc attactgcat tgcttatcaa acgtactttt
     1741 tctcctaatg gccaaccaca aatacctcga atatgggcat cgaactgact catagaacaa
     1801 gcttcaatag aataatgtcc agaattgtga ggacgaggag ctaattcatt cacatatagc
     1861 ccgcctgttt ttgttaaaaa catttctacc cctaaaacac ctgaaaggct cactgcttct
     1921 gcaatcacac gggcaatacg ctcagcctcc tcgatcacat cctgatcaat cgcggctgga
     1981 gcaatcgttt catgaagaat attgttgtgg tggatgtttt ccacgaccgg aaaagtcgtg
     2041 aaatctcctt gaccatttcc tgccaccatg atcgaaatct ctttttcaaa cgggatccac
     2101 gcttctaaaa cacaggttcc ttctcttaaa aggtccatgg caggagctag atccgcccga
     2161 cttttcaaaa catactgccc cttgccatca tagccgcctc gagtggtttt caaaacacaa
     2221 ggatagccga tcccgtcaat cgcatcttgg atatccgtcg gactgacgat cgttgcataa
     2281 ggagcaatca cgatgttatt cgtttccaaa aaagattttt ctaacaggcg gtcttgcgta
     2341 atcgcaagca agtctgtccc ttgaggaata aatgacatgg gtaaaatcgc atttaacgcc
     2401 tctacactga cgttttcgaa ttcataggta atgacatctg ttcttctggc aagctctttc
     2461 agcgcaaagg tgtcatcata gacactttca atgtgccaat ctgcaacttg tgcggctgga
     2521 caatcgctca caggatccag cacacctact ttaaagccca tttcttttgc actaatcgcc
     2581 atcatacgac ctaattgacc gccaccgacg atcccgatcg ttgaaccagg caacaaaggt
     2641 ttaaccaagt tgatcactac tttctataac agttttagcc aacattgaac gtctttcagc
     2701 caatttagcc tccaattcta aatcatacat ggaaagcatc tgtatcgcaa gtagtccagc
     2761 attaattgct cccgcttttc cgattgctgt agtagctaca ggtactcctc caggcatttg
     2821 gacaatcgaa agaagcgaat ccaaaccatt taatgttcgt gattggaccg gtacaccgat
     2881 aaccggcaaa gttgtttttg atgcgaccat acccggcaga tgcgcggctc cccctgctcc
     2941 tgcaataatg attttgattc ctttttttcg agcgttttgt gcatattcaa acatcaaatc
     3001 tggggttcga tgagcagaaa cgaccttttt ttcataagac acaccgaatt catccaatat
     3061 ttgacatgct tctttcattg tttcccaatc tgaagtactc cccataatca cagagatttt
     3121 tgctgtcatt gtttttcctc ccagtttttc tacattcata ttttaacaat cacttttttt
     3181 gatgtcaaag aaatttcgaa tgttaacaaa gaaaaacatt ttattgttcg tgtttattaa
     3241 agataaaaaa aaggaatgtg acaaaagtta ggtcacattc ctttttatgg ataaacggtg
     3301 ttctcctgcc aactcctcgg tcacaaggaa caatattcaa caaacatgag aagctttttt
     3361 ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct tccttcaacg
     3421 gtgttcaatc agctgaccct cggtattagc tcaaacaaac ggcaaaatac gaggaactat
     3481 ttttccgttt gtttctctaa caatcaggtc aaaacgctga tttcacaacc tttcttttgc
     3541 tctattactt cttgaaggct tcttccaata tttcttcatc ttttttcaat ccattaaaga
     3601 tcaaattcaa tatgatagcc gtaatgctac tcattacgat gccatttcca gtgaacattt
     3661 gcaatgtttg cggcatttga ctgaataatg tcggcgtaac attgaaaccc agcccaaatc
     3721 caacagaaac agcaacaatc aatagatttt tatcattgct gaaatcaact tccaacagca
     3781 tacggattcc ttggactgcc accatcccga acatgactaa cattcctcca ccaagtaccg
     3841 gctcagggat gatttgtgct aacgcaccga ttttagggaa taatcctaaa acaatcaaga
     3901 agaatgcaga gaaatagatg ggttttcttg ttttgattcc cgaaagttga accaatccga
     3961 cattttgaga aaagccagta taagggaatg tattgaaaat accgcctaag ataacggcaa
     4021 gtccttctgc acgatatccc tttttcaatt cttcttctcc aacatttttc ccagtaatgt
     4081 caccaagtgc aaaatataca cctgttgatt ctaccatgct gaccatggaa atgatgatca
     4141 ttagtaaaat cgaccatacg tcgaaagttg gtttaccaaa ataaaagggt tgtggaacat
     4201 ggaacaacgg cgcatctcca atcgcgctca gatcaatcat tcccatgaaa gcagctaaga
     4261 tgcttcctcc gactaaacca atcaataccg cgattgaacg aatgaaccct tttgccagca
     4321 tttgcacgcc tacaattagg agaatcgtca caaatgcaag tagcagattt gttttgtcac
     4381 caaaatcagc tgctgttgca ttacctccac ccattttctc gattgctaca ggaatcaaag
     4441 tcaaaccaat aacggtgata accgttcctg ttactatagg agggaatagt tttttaattt
     4501 tagaaaagac accagaaact aacacaacaa agatcccaga agcgataata gatccgtaga
     4561 tagctcctac tccttcattg gttccgatca agatcagtgg agctactgct tggatcgcac
     4621 atcctaaaac gacaggcaag ccaattccga aaaatcggtt aactgtcaat tgcaataatg
     4681 tcgctaatcc acacataaag atatcaattg aaatcaaata agtcatctgt tcttgattaa
     4741 agcctagtcc tgtaccgatc aaaagaggca ccgctactgc tcctgcatac atcgctagca
     4801 gatgctgcag ccctaaaacc gcagcttttc cattttgtgt ttctttttcc acgattatgc
     4861 gtcctcctca agaaactcaa cttgtccatt cgtcaaggaa gcaatacgcg caagagaaac
     4921 gactctcaat cccatttctt caagtaactg acgtccatct tggaaagact tttcgatgac
     4981 gatcccgatt ccttctactt gcgctccagc ttgctggcat aattcgatca atccttttgc
     5041 cgcttgtccg tttgccaaga aatcgtcgat aatcaatact ttgtcttctg aagataaaaa
     5101 tttacgagaa atagaaatcg tacttgtcac ttgtttcgta aaagaataaa cagaagctgt
     5161 caataattct tcatccatcg tcaaactttt tgcttttcta gcgaagatca ttggaacatc
     5221 taattctctt gcagcaaata aagctggtgc gatcccagaa gcttcaatgg tcacaacttt
     5281 cgtgattcct tgttctttaa atacttctgc aaaccgtttt cccatttgtt ccatcaatac
     5341 agggtctact tggtgagtga cgaagctgtc gacttttaat acgccctcgc ctaatatacg
     5401 tccgtctttt tgaatacgtt caactaattc tttcacttta tttgctcctt tatcatttat
     5461 taataccgaa ataaccgctt ttgtgcaaaa acctactatg tttctgttaa agaaaaacac
     5521 cctccacttt atcataagaa aagaagggtg aaatagccaa tcttttactt atagtccagc
     5581 atttacggta ctggctagaa actgtcatcc atattatgac gatatataag taggtatgta
     5641 aaactaataa tagtgtaaat tcattttctt gcaactgttt tgcacgtgaa ggcagtttgc
     5701 catatttgaa taaaaaatgt tgtttcaaat attgaaaaca gtctaacact actttctgaa
     5761 actgacaacg tggtttactt atttttttgt aaaatctaat gatttttaat gcattattcc
     5821 tttttgttcg ggtttaaata gttattttta ttttctccta tctaaaaaag cagcccacat
     5881 aaaaagaagg catgacaaaa agcatgtcat agcccctttt tccgaataaa tagtgttcaa
     5941 aagacagcct atcggcaata aggcaagatt ttataaacta cgggaagtaa tttccgtaaa
     6001 tctattctta ttcttttaag ctgacaagct cgtcaatcac tactgctttt cctgaagcaa
     6061 tagaatgatt tgctgcgatt cctgtcaaaa tcgacaaaat accatctacg tgagttgctg
     6121 cccgattcca cggatcttct tctttttctt caccaaatag atcatctaat aaacgcggat
     6181 ctcctccgcc atgtccgccc gcttcttgtt cgacgattac ctcatatggc tcaccgaaca
     6241 tcggatagac atggatggac tgaagaacgg ctgctccttc atccttttta tctccacctg
     6301 cattgatgta agacttttcg atcactttca tttcgatccg tcctttgctg ccattgaaag
     6361 aaacattgaa tccttcccac ggcatatacg cgtttagcga atacgtcaaa atcgcaccag
     6421 tttcatattt gaccgttacg cctagtgtat cttcaataga gatatcatcg gaaaatacac
     6481 tgcggtcacg aatatatcca ctgtcttttt ctgcttctaa atacatttct cgcaattcct
     6541 cattggctga caggtctata gcaaatggat cagctttagc tagttcgctt ccatagcatc
     6601 gactgtaaaa tatttctact ccgcgtttct ccgcattatg catcccataa aagtttaatc
     6661 caccgaatgc aaataccttt tggggacggc tgttcaacca aaaattcact agatcaaaat
     6721 gatgggtaga tttatgaaca agaagcccgc cgctattgtg cttatttcga tgccatcttc
     6781 tgaaataatc tgccccgtgt tgcgtattta acagccattc gaagtggata gaaaacacat
     6841 caccaatcac atcactttct atcagttcct ttatcttcgt gtgatacggt gcataacgat
     6901 aattaaaagt gactcgcagg cttcttcccg tctgtttttg gaccgttatt atttcttgag
     6961 ctttttcctc atcgatagtc agaggttttt cgcaaataac atcatatcct tttttcattg
     7021 cacgaatgat ataatcatga tgcgttcgat ctactgacgt aataatgact tcagccggcc
     7081 gtacgtcatc cagtagttgc tcaaattgtg atggcataca tgtctttact tttttctgtc
     7141 catacttgtt cacaagtatc cgatttgcat aatccatcct tgtttgagat acatcacaaa
     7201 aaccgactag ttcacaagtt tgatgatacg tagaggcaat tgcctcataa aaaaaacgcg
     7261 ctctcccacc aagtccgact tgtacatatt ttttcttcat ttaaagtcat tctccttttc
     7321 ttttaagcaa aatactgtga caaatggcag ccctggatac agtaatgcag tgagtaaaaa
     7381 atcatgctgt atacaccaga acagtccata caaaaggcat aaataccaca gaaacccctt
     7441 gacgtttttt gacaacagcc agatattcct tttgatcaaa taaacaagac tatctgcttt
     7501 atcttctttg cagatatcaa ttagcgtact aaaaaaacta tgggatacaa aaagcaacag
     7561 acacaataga aaaggaatca gataccaagc tccaatcttt cccactaata aataccaatc
     7621 aactgcaaaa ataatgacaa acagctgaaa caacgtaatg atgaaacaca tccgtttgat
     7681 ttttcctttc ctccaacata cctttttttc tgccaagtaa tggacagata gataataaga
     7741 aggatacatc atcaaaaaat aaatcagata gacaacacga gcagacagca ctaatcctaa
     7801 agtgaacaaa catcctagaa ctaaagcgaa aagagcatta ctgataagac tggttaaaaa
     7861 aatccgacag acatagtagt tgagcttgta aagaaacatc tcttctattg gctttttcat
     7921 attcagccct tcagaccgct tgtactgatc ccctggacaa gatatttatt gaagaataag
     7981 aaaatcagaa aaaccggtag cagcgacaac gtagacatcg caaacatact cgcgtaagtc
     8041 gtactagatc cagcgtctgc aaataatttg atggcaatcg ataccgtgta agatctcggc
     8101 ttgtttaaat aaatcaatgg tcccatgaag tcgtcccatt tccaataaaa ctggatgatg
     8161 atcgtcgtga tgatcgaagg tttcagcaaa ggaaaaataa ttttggtaaa gacactatac
     8221 ttgctgcatc cgtcaataat cgctgattca tctaactctt tggggatgct gaccatgaat
     8281 tgcatgatct ggtaaatgaa aaatgcttgt ccaaaaaaat gtggtaaaat cagcggcaca
     8341 tacgtcccaa ccaaattcag ccgattgtaa ataataaact gcggaatcaa aatcacttgt
     8401 gcaggaatca tcatcgttac taacatgacg acgaaccaga atctttttcc tttgaaatcg
     8461 attctcgata atgcgtaagc aatacaagtc gagctcagaa ctgtccctat cgtcgaaact
     8521 gcagagatga caaacgaatt cctaaagaaa gtaaaaaatg aaattccagc aaaccccgcc
     8581 catccttttg cgtaattttc ccattgaaaa gtctctggaa tcaaattcag cgaaccactc
     8641 ataatctctt gatccgtttt aaaagaacct gcgatcatcc ataagaccgg ataaatcatg
     8701 actagaccta atagcaaaat aaaaccgtat tgaaaccatt tcgttactgt attattcatt
     8761 tcttcctcct ctcattcaca caacgaccca tttttttgat gatttgaaaa tcagaaaggt
     8821 gacagctccc ataatcaaca gcatgatcca cgaaatagca ctagcatacc ccatagaatt
     8881 aaaagaaaaa gcttggttat acacataaag agcataaaag ttcgtggcat cattcggacc
     8941 accttgggta atgataaatg cctgcgtaaa agtcataaac gccgcgattg tctgcataac
     9001 caagttatac aaaatgatcg gcgaaagaag aggtaacgtg atcttgtaaa acgtctgcca
     9061 tttgttcgct ccatcgatct ctgccgcttc ataatacgag cctgggatct ctttgatacc
     9121 tgcagcaaag atgatcattg ccgaaccgaa ttgccaagca gacatcaaaa caagagggat
     9181 catgaccagt ttttgatcgc taaaccagga aagcgcaggt gcgcctaatc gttccaggaa
     9241 aatattcact aacccgtttt tagaaaacaa ctcccgccaa actagagcga tagaaatact
     9301 gccaccgatc aaactaggca cataatacaa agaacgaaag aaagtcgttc ctttgatatt
     9361 cctcgttaaa agataagcaa tcaatagacc aaagatcagt ttcacaggta cagatgcaaa
     9421 tacatagaga aaagtgactc ggatggagtt caaaaaacgg gcatcactca gcatctttat
     9481 ataattatcc aagccgatcc atgagactgg atttcccaaa cgataatcgg tgaacgaata
     9541 ataaagtgac aagatcatcg gtatcactgt tatcaataga aatcctagga tgaatggaga
     9601 agcaaatacg taaccgacca cattgtcttg atccaagaac cgttttagtt tttctttttt
     9661 catgccttcc tcctgctgct atcctagaat gtttgacgca aactcataaa gactctctgc
     9721 tgcttcttct gctgttactt cttcataaat gactttttca actaaaagat tgtattggtc
     9781 ttgaatttcc ggattcaaag cactatcaaa gacattcact tcgccgttgt ccatatcgga
     9841 aatcatttct aaataatcaa atgtcgcttg caaagttgca tcatctgcac gagcgagctg
     9901 ttctcgaata ttcgacatga ttggtacacc acgttctcct tttaaaattt cattggcttc
     9961 ttcactgttt tgaaagaaat caataaactt agcagcttct tctttatggg ctgagctttt
    10021 tgccatactc agcatttgcg aagaacgtaa agggatacca gattctccat ctgcttttat
    10081 gcgaggtgga ttaatcaact gaatctccat atcttcagga gcccctgaca atatttctgt
    10141 caactggtta cttgatagaa acaccattgc cgcttctcta tccctaaccg cctgaaaaga
    10201 cagatccgtc gtacttgcag ctccaccacc tggttctgga tgggcccctt tgtctaccag
    10261 atttttacgc agctggaaaa aatctgttag atagtctgga ttttcaaatc ccaagctgtt
    10321 agatagatct tcccgattat aaaaattcaa tcctttctca acttgtggta tttgaataat
    10381 acagccagct ataaaatctt cgaatcttga aatagcatag attcctaatt cttcagtgat
    10441 ttgatagctg atttgttcaa attcatccca tgtccaattt gaagctggtt cagggatatt
    10501 taattgttca aatatatttt tattgtaagc aatcgcaaac gaattcaccc cgttagacag
    10561 tccaactaat tgcccgtcgt aagtcgtcgt tgctaaatag gattcatctg cattagaaat
    10621 atcaattatc ccttgctcca catattcatc taatggttca atctgattga tgtattgagg
    10681 aaattgattg ttcagttgga aaatgtccca gatatcatct gatgctgcta gtgtacctaa
    10741 ttttgtgtaa tatccttcat aatcataaaa ttcataatcg atcgtcacat ggggattttt
    10801 ttcttcataa agctcgatta cttttactgt tgcatcgtgt cggacttgcg aaccccacca
    10861 agccatgttc agcgtgattt ttttgtcatc ttcttgccca caagccgtca agaagaataa
    10921 cataacactt aaagctaaga tgattctcat tcttttcttc attctctgca cctctacttc
    10981 cattgttatt tttcaatata ctgtattttt atacagaaca tatttattaa acgatctttt
    11041 tcaaggggaa taaaaaccaa gacttgcctt atcgttctgc ctaggatgaa caactcaata
    11101 ctttcaagaa aaattagata gtgctgattc ttctttcatc gctaaaaaca cttcttgctt
    11161 caatgatggg aggataatta cgcaaagaaa aaaggatata agacaactgc ccaatatcct
    11221 tgacatagct aaataagcat gcttcttcgt gtgcgctctt ccctaaaaaa cagaaaaata
    11281 actagtaatt ctgtatgaaa ttaggtacaa tatacacgtc gatgcacaag cattgactat
    11341 ggcaacttga ttggtcagtt cccgctgact tttcaagccc cgacaattta gctgctacta
    11401 aattgtcggg tgcctttttt agtttttatt cgtttttcat tctatagaaa gcgcttcaat
    11461 aaatcaagac atttttttgt tagaatgcta ataataacaa aaggttttaa ctcttcttat
    11521 gtacattaca ggtaaaaaaa caaaaaaatt tctctttttt ttgaattctg aacaaatagt
    11581 actggagtac aaaagacgag gaagagccac tatctgctct tcctcgtctt ttgattctca
    11641 cctgttattc atgattcttt ttctcagttt cccgatattt ttccatttta cgactaaaaa
    11701 gctctccgct acttggtggc gtatatgtga tggcatttgc tcctgcttga atggtctcag
    11761 caatactttc ttccgttgga ccacctgtcg caatgatagg aagttcagga tattttttac
    11821 gaaaatggcg tactgcttct gcagtttctt tacctgcact gatattgata atcgaaatac
    11881 cagctgctaa cttttcatct atttctgtat atttcgacgt taccgtactg ataactggaa
    11941 tatcaactac tcgacaaact tcttttactg tctctaaagg cgtaggaccg ttcaaaacga
    12001 cccctaacga accttgcgct tctgcgaaca tgctcatgta ggaagaacga gcaccttgtg
    12061 tcaaaccacc acctacaccg gagaaaactg gaatgtcagc tgcctcaata atgcttttag
    12121 taatcgctgg atgaggtgta aaaggataca cagcaattac tgcattcgca tttgtattac
    12181 gtatgatcgc aatatccgta gtaaagatga tcgaacggat caattttcca aatatcttga
    12241 tcccgcttgc ttctaaaatg atttccggta ctcttacgat atcttttctc aattccgtca
    12301 tgatttctgg cgtaaattgt tctgccatca gccacttctc ctttgctatc tccggtcata
    12361 ctgattcaat tgatacgtct cgatgacctg gatgattttt tgcgcataac cggggtctgt
    12421 cgcataacct gcatcttgca atgcttgtgc ggcttcctga tagttggtgg ccgtaatcac
    12481 tttttcatac agcgcaggat tccaatctac tccttggacg aacagttgtg tatggtcatc
    12541 catggactgt tcccatgaat catacacttt gaagtctcct tggatagtta tccattcttc
    12601 gttaacaaat tcctttgttt ccaggcttac tttcttctga tccccataag ccttgatccc
    12661 gaaaagatta ttgtatttgc tagccaaggt actttttccc caatttgatt ctaatatagc
    12721 ttgtcctaaa atgatactag gaagcactcc ataaccatct tgtagttctt gggcatgcgg
    12781 aacaagccgt tcaataaatt gctgatggga ttgcgcttca ttttcttgct gattatcagt
    12841 aaaaggagaa gacagattct tcaaggaaaa aacaaaggct aagagtatca ctgctacacc
    12901 ggcaaccact gctggccaac ggatattctt gcgcttttgc tttttccact tttttttcgc
    12961 catgatcaat tcctactttt catttaattc attcagatat tcttctagcg tcaattggtg
    13021 tttggtgata tactcagcgt tttctttacc gacataacgt aagtgccaag gttcatatgt
    13081 gatcttagtt atatcttcgt gtccttttgg ataacgaacg ataaatccat actgcgcagc
    13141 attatctgcg atccatttag ttccaggctg atctccgtaa cttgcatcca atacagtaga
    13201 aggataactg ttgtaccagt tttgatcgac gacatcgact gccagacctg tgtggtgttc
    13261 gctgtatcct ggctccgtaa tcgtttcttt agtaatcttt gtcgcctcct cttctgatac
    13321 accttgacga ctcatcacat cttggacatt ttgttcgaaa acttgttgtt gggatgatac
    13381 agaccggtaa gctgagacca tcactaatgg aaatccagct gcttcagcag ccttggcaaa
    13441 tgcttcatat tctggagcaa tgcgtttgtc gatcaagtat ccgttactta atgatgccaa
    13501 ttgagtagac tcatcaattt ctttttctaa tttgtgatct ggaccaacca aaactagctt
    13561 ccaatcgtct gttttaacat ctggcaattg tttggacgta cgatcttttg tttctttgtc
    13621 ctttaccttg ctgctggttg tttgactgtt cttttcttta ctagcagttg ttttggtgct
    13681 tgttcgttcg actgcttgat tgccggcttg aacatgcgaa tcatcatcgc taaaaataac
    13741 atagcccatt gctgcgatca taatcacaac tgctccaaat cctaaaaatt tattcacttg
    13801 aaaaatcctt tcgttgttct tcacgttccc cattcgcctc atgaacgtaa gtttcttcca
    13861 gattttcatt cacccaatcg agaagttcga ttttttctcc ttcgatctgt ttttgaacat
    13921 ctgctggcag acgaaaattc aaaatatcat catagcccca ttcatcatat tgaatactta
    13981 ctttcaagtc aaggtatcta ccagcctgct gattattttt caaagggatc agctcgactt
    14041 ttgctgcttt gctgctaagc catttttgcg cgatgcgtgt tttgatttgc ttgtactctg
    14101 caacggcaac gtttcgtttt tcgggaaaaa taatcgtttg acgcaaaatc ttttgcgtta
    14161 gcatccattc ataatcagtg aaaagatttt ttactttctg catcgcttca gatggcagat
    14221 ttttctcgcc atctttccat ttttgccatt gttctggact gatcagcaag tattcatcta
    14281 aaaaattttt ttcagtttca aactgttcta ataatacact cataataaga tcaatatata
    14341 cttgatgcac gtttatcccc tccttttaac tattctactt aagttttgat caaaaagcac
    14401 ctcttaaatc gctgctttca ttttttttaa tgtttcaagt cctctcctct ttattcttcg
    14461 tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa ggagcgtttt
    14521 cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca tgagtttgcc
    14581 gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac ttcagaaaaa
    14641 gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta tcaagaaatg
    14701 ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca gcatatcgac
    14761 catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc catcacgatg
    14821 aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt gatcttagca
    14881 gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat aatggataca
    14941 ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa agaaccagac
    15001 cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga tatcggtacg
    15061 tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt tgcctctaca
    15121 atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg taacaaagaa
    15181 aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt cggtatcgtt
    15241 gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg tgcggagatc
    15301 ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga agcaatgaat
    15361 tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg ctctctcttc
    15421 ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa aaatatgtaa
    15481 ttttaaaagg ttgtgaaaaa gctgttctgc atcaattaat aagaacagcc aaacaaaaat
    15541 agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct tttgaacacc
    15601 gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag caatttcaaa
    15661 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac
    15721 accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca
    15781 acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa
    15841 aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca
    15901 gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat
    15961 catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat
    16021 aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct
    16081 gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt
    16141 cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt
    16201 tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat
    16261 ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca
    16321 tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc
    16381 cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta
    16441 ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa
    16501 gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag
    16561 ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc
    16621 ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg
    16681 ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca
    16741 tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca
    16801 aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt
    16861 ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt
    16921 ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc
    16981 gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag
    17041 gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg
    17101 tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta
    17161 acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc
    17221 ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat
    17281 tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac
    17341 tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag
    17401 agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa
    17461 taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg
    17521 tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca
    17581 tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag
    17641 tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa
    17701 tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag
    17761 attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg
    17821 ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt
    17881 tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct
    17941 aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg
    18001 gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg
    18061 gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt
    18121 tcttccagtt cgatttgttt ttgacgttct acgattttaa tctgcatttc ctgttcgatt
    18181 acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca
    18241 acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg
    18301 atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg
    18361 atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg
    18421 attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg
    18481 aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt
    18541 tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag
    18601 cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct
    18661 aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa
    18721 gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt ggagacgtcc
    18781 aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg
    18841 ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt
    18901 cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct
    18961 acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca
    19021 actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg
    19081 acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc
    19141 tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac
    19201 tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc
    19261 agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag
    19321 aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa
    19381 gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa
    19441 gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc
    19501 aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg
    19561 attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa
    19621 aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat
    19681 cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga
    19741 ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc
    19801 ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt
    19861 ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg
    19921 ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc
    19981 aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc
    20041 ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc
    20101 atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt
    20161 tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag
    20221 tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga
    20281 ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc
    20341 aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg
    20401 aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa
    20461 ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg
    20521 atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat
    20581 tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc
    20641 tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa
    20701 tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc
    20761 catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga
    20821 tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa
    20881 agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg
    20941 caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat
    21001 ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact
    21061 tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca
    21121 tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg acggtgtcgg
    21181 gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa
    21241 tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga
    21301 attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga
    21361 tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc
    21421 gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag
    21481 aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc
    21541 ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag
    21601 tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg
    21661 ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt
    21721 atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc
    21781 aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag
    21841 gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt
    21901 tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt
    21961 tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc
    22021 ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt
    22081 gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact
    22141 tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg
    22201 ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct
    22261 cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg
    22321 ttacaagaac tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct
    22381 cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa
    22441 aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc
    22501 cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga
    22561 tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg
    22621 ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa
    22681 tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta
    22741 tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct
    22801 catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa
    22861 gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac
    22921 gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag
    22981 tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg
    23041 ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat
    23101 gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa
    23161 aatctatctc gttcggacga acaccgtaac atgcgattct catactttct tcctccattt
    23221 cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca
    23281 gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat
    23341 caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga
    23401 ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa
    23461 tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt
    23521 tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg
    23581 ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct
    23641 ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt
    23701 gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg
    23761 gattgacgaa catcgctttg atatttttag cttccatctt caagccactc atttttatgt
    23821 atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc
    23881 cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga
    23941 tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg
    24001 aaactagaat aagaataata taaataacta atccccaaat caagacattg attccttgca
    24061 tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca
    24121 ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc
    24181 tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca
    24241 tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt
    24301 tcatttattc ac
//
LOCUS       DAXWLB010000025        24269 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXWLB010000025 DAXWLB010000000
VERSION     DAXWLB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.025"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..570
                     /locus_tag="ACGE4H_000777"
     CDS             <1..570
                     /locus_tag="ACGE4H_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFF8364216.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            574..1215
                     /locus_tag="ACGE4H_000778"
     CDS             574..1215
                     /locus_tag="ACGE4H_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364217.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1212..2129
                     /locus_tag="ACGE4H_000779"
     CDS             1212..2129
                     /locus_tag="ACGE4H_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8364218.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2126..2797
                     /locus_tag="ACGE4H_000780"
     CDS             2126..2797
                     /locus_tag="ACGE4H_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364219.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3338..3826)
                     /locus_tag="ACGE4H_000781"
     CDS             complement(3338..3826)
                     /locus_tag="ACGE4H_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFF8364220.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4027..4293)
                     /locus_tag="ACGE4H_000782"
     CDS             complement(4027..4293)
                     /locus_tag="ACGE4H_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8364221.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4293..4556)
                     /locus_tag="ACGE4H_000783"
     CDS             complement(4293..4556)
                     /locus_tag="ACGE4H_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8364222.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5077..5217
                     /locus_tag="ACGE4H_000784"
                     /pseudo
     CDS             5077..5217
                     /locus_tag="ACGE4H_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5359..7182)
                     /locus_tag="ACGE4H_000785"
     CDS             complement(5359..7182)
                     /locus_tag="ACGE4H_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8364223.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7780..10485)
                     /locus_tag="ACGE4H_000786"
     CDS             complement(7780..10485)
                     /locus_tag="ACGE4H_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFF8364224.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10791..11738)
                     /locus_tag="ACGE4H_000787"
     CDS             complement(10791..11738)
                     /locus_tag="ACGE4H_000787"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFF8364225.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11928..12197
                     /gene="rpsN"
                     /locus_tag="ACGE4H_000788"
     CDS             11928..12197
                     /gene="rpsN"
                     /locus_tag="ACGE4H_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFF8364226.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLHKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12373..12735)
                     /locus_tag="ACGE4H_000789"
     CDS             complement(12373..12735)
                     /locus_tag="ACGE4H_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8364227.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12746..13867)
                     /gene="alr"
                     /locus_tag="ACGE4H_000790"
     CDS             complement(12746..13867)
                     /gene="alr"
                     /locus_tag="ACGE4H_000790"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFF8364228.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13883..14233)
                     /gene="acpS"
                     /locus_tag="ACGE4H_000791"
     CDS             complement(13883..14233)
                     /gene="acpS"
                     /locus_tag="ACGE4H_000791"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFF8364229.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14363..15874)
                     /locus_tag="ACGE4H_000792"
     CDS             complement(14363..15874)
                     /locus_tag="ACGE4H_000792"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFF8364230.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16196..17548)
                     /gene="murF"
                     /locus_tag="ACGE4H_000793"
     CDS             complement(16196..17548)
                     /gene="murF"
                     /locus_tag="ACGE4H_000793"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFF8364231.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17701..18777)
                     /locus_tag="ACGE4H_000794"
     CDS             complement(17701..18777)
                     /locus_tag="ACGE4H_000794"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFF8364232.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18973..20718
                     /locus_tag="ACGE4H_000795"
     CDS             18973..20718
                     /locus_tag="ACGE4H_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFF8364233.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20998..21435)
                     /locus_tag="ACGE4H_000796"
     CDS             complement(20998..21435)
                     /locus_tag="ACGE4H_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFF8364234.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21419..22792)
                     /locus_tag="ACGE4H_000797"
     CDS             complement(21419..22792)
                     /locus_tag="ACGE4H_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364235.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23114..23602)
                     /locus_tag="ACGE4H_000798"
     CDS             complement(23114..23602)
                     /locus_tag="ACGE4H_000798"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364236.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23624..24226)
                     /locus_tag="ACGE4H_000799"
     CDS             complement(23624..24226)
                     /locus_tag="ACGE4H_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8364237.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4486 g         7661 t
ORIGIN      
        1 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat
       61 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
      121 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
      181 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
      241 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
      301 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
      361 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
      421 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
      481 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
      541 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
      601 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
      661 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
      721 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
      781 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
      841 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
      901 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
      961 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
     1021 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
     1081 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
     1141 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
     1201 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
     1261 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
     1321 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
     1381 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
     1441 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
     1501 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
     1561 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt
     1621 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
     1681 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
     1741 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
     1801 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
     1861 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
     1921 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
     1981 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
     2041 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
     2101 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
     2161 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
     2221 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
     2281 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
     2341 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
     2401 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
     2461 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
     2521 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
     2581 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
     2641 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
     2701 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
     2761 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
     2821 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
     2881 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
     2941 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
     3001 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
     3061 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
     3121 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
     3181 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
     3241 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
     3301 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
     3361 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
     3421 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
     3481 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
     3541 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
     3601 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
     3661 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
     3721 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
     3781 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
     3841 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
     3901 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
     3961 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
     4021 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
     4081 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
     4141 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
     4201 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
     4261 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
     4321 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
     4381 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
     4441 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
     4501 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
     4561 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
     4621 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
     4681 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
     4741 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
     4801 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
     4861 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
     4921 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
     4981 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
     5041 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
     5101 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
     5161 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
     5221 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
     5281 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
     5341 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
     5401 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
     5461 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
     5521 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
     5581 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
     5641 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
     5701 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
     5761 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
     5821 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
     5881 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
     5941 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
     6001 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
     6061 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
     6121 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
     6181 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
     6241 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
     6301 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
     6361 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
     6421 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
     6481 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
     6541 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
     6601 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
     6661 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
     6721 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
     6781 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
     6841 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
     6901 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
     6961 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
     7021 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
     7081 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
     7141 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
     7201 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
     7261 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
     7321 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt
     7381 tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt
     7441 tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac
     7501 tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg
     7561 cggtaataag ctcaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat
     7621 tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc
     7681 ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct
     7741 aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc
     7801 atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc
     7861 tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg
     7921 ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa
     7981 cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc
     8041 agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc
     8101 agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct
     8161 gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa
     8221 gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt
     8281 cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag
     8341 aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt
     8401 atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact
     8461 ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc
     8521 gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt
     8581 gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac
     8641 tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc
     8701 agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc
     8761 ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg
     8821 gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt
     8881 cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct
     8941 cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt
     9001 ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc
     9061 atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt
     9121 caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc
     9181 aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac
     9241 cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt
     9301 gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt
     9361 atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac
     9421 actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt
     9481 tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg
     9541 ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat
     9601 cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat
     9661 tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt
     9721 caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt
     9781 tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt
     9841 tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt
     9901 attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa
     9961 agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc
    10021 tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt
    10081 tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa
    10141 gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc
    10201 aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat
    10261 ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag
    10321 ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga
    10381 agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt
    10441 ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct
    10501 tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc
    10561 aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt
    10621 gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga aagataattt
    10681 tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt
    10741 tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc ttatttttcg
    10801 aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg tgcttggatg
    10861 acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc tcctttttgg
    10921 taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct tggaacaaac
    10981 gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc atttaatgca
    11041 acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat acttcctttc
    11101 gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt tgttttatca
    11161 taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt actggatgca
    11221 ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga cccgaccgca
    11281 cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa atggttgatt
    11341 agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca actcatttta
    11401 tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta agtcccgccg
    11461 actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga accaatctct
    11521 gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt tcgtatctct
    11581 ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc ttttatttct
    11641 acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc aaaaacatct
    11701 tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc cctttttctt
    11761 taagtttacg tttatgtctt tattttgaca aaaaaaatgt cttttcgttt tggcgcatga
    11821 ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat ctaaaagcgt
    11881 aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg gctaaaaaat
    11941 caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct gctttacgcc
    12001 aggaattaaa agcgcaaaaa gattacgaag gcttgcataa acttccttta gatgctcgtc
    12061 cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac atgcgtaaat
    12121 tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt ccaggagtaa
    12181 aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc gcatggacca
    12241 ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct ggtactagaa
    12301 gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg ctgcatggtt
    12361 acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac taattttcaa
    12421 tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt ctttcaatct
    12481 tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt ctattcctgt
    12541 tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt ttgccgttat
    12601 ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa taaccaaaac
    12661 tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa aataaatatc
    12721 tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc cgctgtccta
    12781 atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc tgcatagtga
    12841 tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga aattcttgag
    12901 gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag tagttccctt
    12961 ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa ccgatcggta
    13021 tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg attccttcac
    13081 ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg
    13141 gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct actccataac
    13201 gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg ctgacatgta
    13261 catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct ttttgcagat
    13321 tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt
    13381 ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa
    13441 atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc gtctctagct
    13501 tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct gcagttactg
    13561 ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc aagataagga
    13621 tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt gaaacacaaa
    13681 agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca tgaccgtatc
    13741 cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca acttcatttg
    13801 ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg tgccaagcga
    13861 caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat
    13921 ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc acaacaggct
    13981 gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg atcccagttc
    14041 cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat tctacttggc
    14101 gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt cgtgcaatga
    14161 attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca tcgatcccaa
    14221 ttccttttat catatgcttt tctcttttca ataaatttag aacactctca ttctaccaaa
    14281 ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt tctcttaaaa
    14341 aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt
    14401 tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt
    14461 ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac
    14521 gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct
    14581 tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat
    14641 cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta tctagaccat
    14701 tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt
    14761 ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat
    14821 gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac
    14881 ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt
    14941 acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga
    15001 tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat
    15061 cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta
    15121 cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc
    15181 gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt
    15241 cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc
    15301 caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag
    15361 gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg
    15421 cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt
    15481 caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct
    15541 gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta
    15601 aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac
    15661 cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg
    15721 ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa
    15781 tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca
    15841 attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac
    15901 tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt
    15961 ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc
    16021 actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt
    16081 atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct
    16141 ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt
    16201 tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca
    16261 taatactatc tgttgctgca agattttgtt tgatagcatc aatcaattta ttcttttctt
    16321 tttcaaaata gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat
    16381 gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca
    16441 tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct aacacagcga
    16501 ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg
    16561 gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg
    16621 ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc
    16681 caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca
    16741 gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg
    16801 tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg
    16861 ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata
    16921 caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt
    16981 ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa
    17041 gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt
    17101 ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct
    17161 tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac
    17221 cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca
    17281 attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt
    17341 gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa
    17401 attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc
    17461 ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt
    17521 gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa
    17581 gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg
    17641 tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct
    17701 ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat
    17761 cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat
    17821 actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt
    17881 tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt
    17941 cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc
    18001 gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc
    18061 accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc
    18121 ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt
    18181 ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg
    18241 tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt
    18301 ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg
    18361 taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc
    18421 gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg
    18481 cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac
    18541 ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc
    18601 tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat
    18661 aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga
    18721 aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag
    18781 attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa
    18841 aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat
    18901 ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa
    18961 ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg
    19021 ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa
    19081 aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga
    19141 gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct
    19201 agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca
    19261 gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat
    19321 cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc
    19381 gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt
    19441 cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc
    19501 aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat
    19561 ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc
    19621 gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact
    19681 gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta
    19741 gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca
    19801 gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact
    19861 gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa
    19921 gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct
    19981 gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat
    20041 cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg
    20101 ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc
    20161 aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc
    20221 gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc
    20281 ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc
    20341 actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg
    20401 aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga
    20461 caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt
    20521 atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg
    20581 cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg
    20641 tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat
    20701 aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa
    20761 ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag
    20821 gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa
    20881 gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
    20941 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta
    21001 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
    21061 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
    21121 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
    21181 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
    21241 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
    21301 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc
    21361 aagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
    21421 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
    21481 tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
    21541 gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
    21601 ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
    21661 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt
    21721 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
    21781 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
    21841 ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
    21901 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
    21961 caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa
    22021 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
    22081 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
    22141 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
    22201 ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat
    22261 gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa
    22321 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
    22381 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
    22441 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
    22501 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
    22561 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
    22621 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
    22681 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
    22741 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
    22801 ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa
    22861 ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc
    22921 tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc
    22981 cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa
    23041 tcaattagga gctctgtaaa ccatagtcct tctcgacccg tcgcataaat ttactttaac
    23101 acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt
    23161 gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga
    23221 ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag
    23281 caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta
    23341 gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga
    23401 cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa
    23461 tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg
    23521 gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt
    23581 ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg
    23641 cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat ggtttcaaga
    23701 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
    23761 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
    23821 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
    23881 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
    23941 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
    24001 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
    24061 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
    24121 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
    24181 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
    24241 tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DAXWLB010000026        24115 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXWLB010000026 DAXWLB010000000
VERSION     DAXWLB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.026"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1435)
                     /locus_tag="ACGE4H_000800"
     CDS             complement(<1..1435)
                     /locus_tag="ACGE4H_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364238.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDK"
     gene            1557..2141
                     /locus_tag="ACGE4H_000801"
     CDS             1557..2141
                     /locus_tag="ACGE4H_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364239.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2152..2397
                     /locus_tag="ACGE4H_000802"
     CDS             2152..2397
                     /locus_tag="ACGE4H_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364240.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2403..3125
                     /locus_tag="ACGE4H_000803"
     CDS             2403..3125
                     /locus_tag="ACGE4H_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364241.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3256..3999
                     /locus_tag="ACGE4H_000804"
     CDS             3256..3999
                     /locus_tag="ACGE4H_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364242.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4005..4370
                     /locus_tag="ACGE4H_000805"
     CDS             4005..4370
                     /locus_tag="ACGE4H_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364243.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4386..4916
                     /locus_tag="ACGE4H_000806"
     CDS             4386..4916
                     /locus_tag="ACGE4H_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364244.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5078..5386
                     /locus_tag="ACGE4H_000807"
     CDS             5078..5386
                     /locus_tag="ACGE4H_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364245.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5493..5801
                     /locus_tag="ACGE4H_000808"
     CDS             5493..5801
                     /locus_tag="ACGE4H_000808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364246.1"
                     /translation="MYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVPMLNSMS
                     RFLKEREKKELQYIKEIMPDFEMHEWQIALSDWKLANKVRRITPFQAKRFLRAMKFVQ
                     "
     gene            5938..6738
                     /locus_tag="ACGE4H_000809"
     CDS             5938..6738
                     /locus_tag="ACGE4H_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364247.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6775..6957
                     /locus_tag="ACGE4H_000810"
     CDS             6775..6957
                     /locus_tag="ACGE4H_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364248.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7068..7385
                     /locus_tag="ACGE4H_000811"
     CDS             7068..7385
                     /locus_tag="ACGE4H_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364249.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7388..7531
                     /locus_tag="ACGE4H_000812"
     CDS             7388..7531
                     /locus_tag="ACGE4H_000812"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364250.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIVQMFRLPVSE
                     LKK"
     gene            complement(7735..8214)
                     /locus_tag="ACGE4H_000813"
     CDS             complement(7735..8214)
                     /locus_tag="ACGE4H_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364251.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8298..9671
                     /locus_tag="ACGE4H_000814"
     CDS             8298..9671
                     /locus_tag="ACGE4H_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFF8364252.1"
                     /translation="MKKSKRKIATLLMLMVQFTGIAMSVITINPQVIHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRAVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQ
                     GQCTELTWAYMSQLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWA
                     VGDAGHTGVVIAVFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            9694..10221
                     /locus_tag="ACGE4H_000815"
     CDS             9694..10221
                     /locus_tag="ACGE4H_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364253.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10208..10771
                     /locus_tag="ACGE4H_000816"
     CDS             10208..10771
                     /locus_tag="ACGE4H_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364254.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11193..11930
                     /locus_tag="ACGE4H_000817"
     CDS             11193..11930
                     /locus_tag="ACGE4H_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364255.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(11947..12363)
                     /locus_tag="ACGE4H_000818"
     CDS             complement(11947..12363)
                     /locus_tag="ACGE4H_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364256.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKYKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12755..14002
                     /locus_tag="ACGE4H_000819"
     CDS             12755..14002
                     /locus_tag="ACGE4H_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8364257.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14270..16660
                     /locus_tag="ACGE4H_000820"
     CDS             14270..16660
                     /locus_tag="ACGE4H_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8364258.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16709..18544
                     /locus_tag="ACGE4H_000821"
     CDS             16709..18544
                     /locus_tag="ACGE4H_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8364259.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18619..19476
                     /locus_tag="ACGE4H_000822"
     CDS             18619..19476
                     /locus_tag="ACGE4H_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8364260.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            19466..20575
                     /locus_tag="ACGE4H_000823"
     CDS             19466..20575
                     /locus_tag="ACGE4H_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8364261.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            20914..21714
                     /locus_tag="ACGE4H_000824"
     CDS             20914..21714
                     /locus_tag="ACGE4H_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364262.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            21820..22608
                     /locus_tag="ACGE4H_000825"
     CDS             21820..22608
                     /locus_tag="ACGE4H_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364263.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(22625..24070)
                     /locus_tag="ACGE4H_000826"
     CDS             complement(22625..24070)
                     /locus_tag="ACGE4H_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFF8364264.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KTKKPMAFYFKIMQRLTKRSGLITNSFLSTI"
BASE COUNT         8871 a         3396 c         4684 g         7164 t
ORIGIN      
        1 ctttatcctt taattgaaaa ccctgatcca tagtcactgc atttggatat agaagcccac
       61 tataatgttt acgtaaattc acttggaatt tctgattata aaagtctaac agattagaaa
      121 taaaaggcgg aaaattactg tttttgtaaa attgattatt tccatttagg ttgtatagta
      181 atttgttaaa ttgctcctga tttaatttat tgtaaatcca tggtaatact tccatatcct
      241 tatctgctaa cctgaatgaa ctagcatcgg taagatgttc gatcataaaa tctatgaaca
      301 tctctttttc accaaaatat aaatattgat tatatattgt ttctaatgtt gaagatggta
      361 gcgaagaaac tctcattttt tgctggagta taggatcaac tagactattt aagtgatttt
      421 gtaattcttc attgttgttt cctagaactg caagggaacg taaacggtag taatattcta
      481 tttctgttag tacttgttgt ttcggtttaa aatcatttat tgatatttgt aaattttgaa
      541 ttgtctcgtc tgaaaaatca tgttgtaatt ctggatgggt agctataaaa gtaagtaaaa
      601 agaacccttt tgtatacgtg atctgttgat tttcaccaag tgatttatta atttcctctc
      661 ttgattcagc tgtgaaccta tactttgcaa tttctttttt tagaatattt aaatagaatt
      721 ctttattatc aaaaattagg gcattaagta gtctgaaagt actttcccta tctctatctg
      781 cttcctcatt tccataagat aattgaaaaa caaacttaaa ggctgtctta cataggtttt
      841 ctttggcttt ttcgcttagt ttatttagat agctattttt tagtgcagag tgaaattctt
      901 caggtgtaac agacatagca tgtatgcgac ttgcatattg tataatttcg atcgttactt
      961 tttttcctaa tattggattt tttaggaata taatttcata ggacttccgt ataacatcct
     1021 gacaagtttc ttttgttata tctcttaatt cccaagtatt accgttgtaa gtgactacag
     1081 gatgagctga ataatttctt tgatccttaa tatattttaa atcctcatat tcatagctat
     1141 caattagctc agttgaacta taagcttttt ctagtagttc catttcccat tttggagagt
     1201 ttggatcact ttttcttagt ctagctatat cttctaaaat tttcttagaa gtattatcct
     1261 cgtaaatatt tgctaaatca gataatttat caattaaatc taaaataagc gcactgtaat
     1321 tgacagctat tgcagaacga taatttccct gataaaaact acttctaact tcttctaata
     1381 atggaataat tctaggattt ttaacttttt ttatttcatc atctaatata aacatataaa
     1441 tctccttttt ttatttgtat gacaagttta ccataaaaaa aaattaaaaa gcgagacata
     1501 tttatgttgt tgtgttatca taataacata aatatgtaga gtgagatgat gaaaaaatgg
     1561 atttgaaaca atcgttttac atctggttat atcctgataa tcctatgtca ataatggctg
     1621 atatttttgt agttagcatc ttattgtatt tattttatct aattttcttt cacaatttaa
     1681 tcgggtacat gattcttgat tggaaaggaa tgttcgatat agaaagaaaa ccatttgcta
     1741 acaatcaaac ctataccgtc ttcgaaagtg gagattggga agaaaatgaa gttgatgtga
     1801 tccatgaaat tggattagct ttatcaggaa tacaagatgc tgatgttttg gtatcaatga
     1861 caacggatac tggatacaac gcttatgagg tattttatct tatgtatttt atatggcgtg
     1921 atggtcaaga aattatcaat ggtcgtgctg aaaaaacaat caattctaat actgaaattt
     1981 ctttttgtat gggtgaaggg gaaatgattg tatataaaga tgggtcagca aacattaaaa
     2041 cagcctttgg tgatattgaa acaagtatta aggaacttag ggaatatgtg cagaacccat
     2101 ataattttca atatttagca ggaagaataa atttagttta ggagtaaaaa tatgaaaaaa
     2161 attataaatc aaccaacaaa tcacttgata attgaaggtg ttattttcgt aacaatgtat
     2221 tttactgtag gaaatggcat ctcaaataca atcattcaaa aaaatccaca atacctatta
     2281 tttagtcttt atgcttggat tgtacaaatg gtttgggttt ccatattgat gctgttgttt
     2341 tattggatag tacgggttat tcttttattg ttaaacaaaa gaaaaaagga taggtgaata
     2401 aaatgaaatc aactaaattg aaggttgtat tgttatcgac agcagcatta ataaccctta
     2461 cagcatgtgg tacaactaat agtgctaata ataacaaaga cacaaagtct agtagtacga
     2521 tggttactaa aaacagcact acatcggcat caacaacaat gtcttcaatg actgaaaata
     2581 atgctgaaaa gtatcaaaaa tacgaagaca ttaataaagg tcaaaagccg gatgaagttt
     2641 ataacaactt tattaataca gtaaaaaatg atgaaaatta tgggaatgat ccattgcaaa
     2701 ataaaaatct gactatcgtt ctttacaaac ctgattgtcc agattgtcaa aaagcagaat
     2761 caaaaatcat gcaagaaata aaagaaggat ttagttatgc tccaacatta atgcatgata
     2821 ttaccataaa taaaccagct tacgagtatt acgcagttgc tgtagatatt actgatggtg
     2881 tacctgagtg ggtacaaaac attcctggtc tggctgatga gaacaatcaa tatcatacgc
     2941 caacagttgc agtagtaaca ccaaagaaag ttaacggcac actatactgg gatgtaatgg
     3001 gaatgacaac aaatacaact gatgaaatga ttgaaagtac aactaaatat tatatggatt
     3061 tgggagcata taaattatat caacaatcag agcctgttat ggacgatcaa tccaaagtta
     3121 aatagatctt tagggcgtaa gccctatttt tttaaaatta agtgataaca aataaatgat
     3181 ttctgaaaca aacgttctta ctcaactact cgaaacacac aagctaatta actagattat
     3241 aaaggagaat gcgtaatgaa agaaatcagt gcaaatgaag ctcatattcc gcaaaagaat
     3301 caggttggta tatataaggt gcttcatggt gaaggaattt ataaaataca gttacttacc
     3361 ggttacaata atggtgcaaa tgggaagtgg ttaaaggcaa caagtattcg tgaacttaga
     3421 agtgaagtag aaaaaattag gcttggtctc acaagtgaag aaatagctaa actgaaggga
     3481 aataattctt ttgtaattga aaaagaaaag gaaaaacatc tactttatcg tgagctgaaa
     3541 attgaagaat actatgaaat tgatttacct gaaaaattaa gaattattga tcaacgttca
     3601 gaagaaaaat ggaaaactga aatatatcca agcttaaatg actacaagac atcaggaaag
     3661 cccatgtata actacacagg ctttttaacc tacacacaat acaaatatgc ctttcgcaaa
     3721 ttaaaaggca ttaaacaagc tgaaattgcc ctgtatgaga tgaaaaaagt ggatagattg
     3781 aaattgattc aagatttagt gcaaaataca tggaatcaag cgattgtcga gctagaaaaa
     3841 gaaaatggga aaattttaac taataaatat ctgatcgtaa atgaaattga agaattaatt
     3901 ggtttatttt catgtgaaaa aggtgaagtc ttcttgaagt catcaccaac ctattcaatt
     3961 ttggacagtc cgtatcagtt tatacaggca aggagatgag ttagatgtct agcttgaaag
     4021 atgactatat ccaagcaaaa caagagtatg aaaaaataca gatgttgaaa tctgagaaag
     4081 tcgaattatt tttaacacaa aaattagcag aggatcatat tgctaactta gatgctaaaa
     4141 ctttagagga atatgatcgg cagtttaatc agatacatca caatatcatt ttgcttgttg
     4201 agcaggcaaa gcgaaggttt gaaaaagcgg aatatgatct attagatcaa ggaattgtat
     4261 gcttggataa gggattgcga agatattatg gtgcagagtt aaaaaagcaa aatgtagata
     4321 gaaatgcact aaaacatgaa atcttagaat tgataatgca acgaatttga agggatagaa
     4381 agcaaatgtc acaaaacatt agtgctgtaa aacaattaat agaagaagat ttagagacca
     4441 cagttcagag attagcggaa cgaatccatt ttgcaaatta tcaaaccatc tctaactggt
     4501 caagcaggaa cagaaatccg gaagaaattc caatgaaatt tttttatcga atcggaaaag
     4561 aatatggtgt tgatccgata aaaattttcg agaagaccat gatttattac tatcatggaa
     4621 cacctaacga agtgttagaa catcttttgg aagaggcaaa agaaaaatca tcaatattag
     4681 agctatcgca aaatgacaaa gttattcaag tgaaatgcgt tgatccaaca ggaaagatta
     4741 ttgtcggttt tgatattgaa agaaaatcca aaatagattt tattacacaa ttaaaaaaga
     4801 taaataattt agatattcta acgcttagac cagatcatga acagttgttt atgagtttta
     4861 aaaaacaatt gaaagaaata caaaaaagat ataacaaggt gtttcctaat gcgtgaaaag
     4921 ctcaaagaaa tcagagtttt ttggataatt aacaaagaat gtatcatctt atatactctt
     4981 ggatttctta gcatgatctt gttttggtgg ttatatttaa aacagtaatt ggagagggtc
     5041 aagaggattt ttatcaagat aaggatgtgg aaactgcatg tggaataata gattaaaaac
     5101 aggattgtta ttaatagtta tttcctgcgc tatgatgatt ggtatgagga tacaaaggga
     5161 acaatcctat tttgaagttt ctgctaacaa tgtaatagaa agatgccatt atggtcaaca
     5221 tttttggact ggggaagtca gagaaaatat agatcgtgaa tatgttcaaa gaattgtttg
     5281 ggatgcatat tctatcaaag attatcctaa aagtttaacg agtagattat tttacagtga
     5341 aaaagataat caaaaactct cagatttgat gatgaaaaaa gtatgactat tattacgaaa
     5401 gagaaacaga gtgctatatt cgaggggaaa aagcctacaa caaaagtgac tattggggtg
     5461 acaaggcgaa gcgattaact gcaaatttta gagtgtatga atcagagttt ataaacttga
     5521 cctttcttaa tagtgttttt attgattatg taattactac gaaacgcatt ggaagttatt
     5581 caagtaccta cgcatcctta gttccaatgt taaactcgat gagccgtttc ttaaaagaaa
     5641 gagaaaagaa agagctacag tatatcaaag aaatcatgcc ggattttgaa atgcacgaat
     5701 ggcaaattgc tttatcagat tggaaattgg cgaataaggt tcgtagaata acaccttttc
     5761 aagccaagcg ctttttgaga gctatgaaat ttgtgcaata aaaaaagcca ccagacaagt
     5821 tgatggagac taacacaatt accaacagtt gtaattgtgt tagcttacaa attaattatg
     5881 gtgtttagtc aatcccaaat tgtgaaatgg caccaataat tggaaaggaa aaatgaaatg
     5941 tctgaagatt tacgagagct gaagttacga aagaagcagt atgatcagta tttttccggt
     6001 ttgttgaata aacatattga agaaggactt gatcatgatt ttggaaaaga tgaatattgt
     6061 ttatattgcg aacgttacaa tgaaaaaatg tctgaatatc tacagctgaa aaaagagata
     6121 cagaaattag agaaaaaagt acatgcttgg aagtataaag attccatgtc tggttatgtt
     6181 gaatctcaaa ataagccaag aaaaaagaag caagcgtatg ttctaaagat ggtaaatggg
     6241 gaagagttaa ttgtattagc gtacagtgaa gaagaggttc gagaactcgt tgaaacacct
     6301 gtcaggaaaa aggttgaaac aataaatgag atagacgaca gccaatacaa tagcatctat
     6361 attgaaacaa gtcatggtgg atataagaaa ttagcaactt tccttagaag ttctaaagtt
     6421 gggataattt ctaaaaaaga cataagtgct gttaccgata gtttttgtac tttatctgtt
     6481 cctaaagctt atttccaata cgatagtaaa agatttgaac gcttagagaa attctgtaga
     6541 ccgtatactg tattgaaaac taaaaaattt aataaaacta aaagagtttc tatacagtta
     6601 ccgactggtc aaatctcatt aggtgaaaaa gactatctga taaaagtagg aaaagagtta
     6661 attataaaag taccggaaca cttatttaaa gaaaattttg agatacgtga aaccttggta
     6721 tggaaagaag aaaaatagtt tggaagacat agaatggtat aaggggaaat tgaaatggca
     6781 gtaaataaaa tattgcaggt actaatcata gacgaatttg gcggaggaga atggtttgat
     6841 gtgcagtcca tagactttga agaagaagtc gtagttatag aaaatattta cggcgaagta
     6901 tattcatatt cgcttgaagg tgagaatatt gaggatttac ggttgattgg tgagtagaaa
     6961 aaaacagaaa tatcaaaatg gagataaaaa ttaatagcat gtaaagatag tcatgcttgg
     7021 gttaaaaatg ttaaaattca aaaaaatgag tcagaaggga taaaagtatg aaaagagagg
     7081 aatacaaaca aagattaaac gaactgcttg aagaggacga aacattaact catggttcgc
     7141 ctgatgaaat tctatacatg attgataata tggtgatctt tggtggatat gaacttggta
     7201 ataggtcagt tgatcataat attttagaat ttgatgatgt atcatgggaa gaaattcttg
     7261 attggggaat tttggcggta cctgaaacta aaacctatat tagtgacact atggttcctt
     7321 tttttgagga attggattat aaaagacttc ccaaaaatga aaatcatata ctgggaggaa
     7381 actaacaatg ttaagtgact tatttatttt actgatctat ctaggtgtaa gtgaaattat
     7441 tgcgtatttt ttgtttgtca ttgctaatta tggtatttcc agtgcaatcg ttcaaatgtt
     7501 tagattacca gtaagtgaac taaaaaaatg atgagattat cagcatatct tttaatgttt
     7561 ttgaatgtgg tcacgttaat ctttgtagta atcggtgtga ctttcgattt aatggaatag
     7621 tgggaaatct ataatggaaa aaagagacac gaatgaacat attttttgaa tcaatattgt
     7681 tgatggtttt tgcgatcagc gcttaccaga aatctaaaaa agagtaatga ttgtttaatc
     7741 atcactcttt ttttgtaggg aaattgaaat atgtggagct tgtattggtt tagttaggat
     7801 tgcttggcta taaggagcta gttctttatc aaaaccatca aagtaggttt tcagtagcga
     7861 tttaactgat cgctcatctt gtttgatttg gataatttca gcatccaaat cggttgtgag
     7921 atcaggagct tttgatgcga ttacctgatt ttttttatct tccaactctc ttaggctgtt
     7981 taacaagttg taaaaagaat cattttgatt gttcattcca acgtgagttg caattttaac
     8041 agaagtttcg tccgtattgt catcaaaaga actcattgga atatcaagta gtaacgagga
     8101 tgtttctttt actgactttt tctttacagc attttttgtt tttccatctt ttttgctctt
     8161 tactcgagat gtttttggag ctagtttagg taaatcactt gttttatcag tcatttatta
     8221 gcttttcctt tctattaatt tatgttatta tattatcata ataacataaa aaagtacact
     8281 ttttgggggc gtagttattg aaaaaatcta agagaaagat tgccacattg cttatgttaa
     8341 tggtgcaatt tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgatacatg
     8401 ctgatgggat agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag
     8461 gaacagatag cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa
     8521 atattgaaga tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg
     8581 caggtatttt aggtaactgg aaacaagaat caggtattga tcctcgagca gtagaagggt
     8641 attacggaga tggacagaga gaagttgact tcatcaaaca atttactgat ggtctgatgg
     8701 gtattggcta tggtcaatgg acagctgggc gacatacagc attagttgat tggtctaaga
     8761 aaaataatgg cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg
     8821 atgggggatt cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtcgtag
     8881 aaaatgcagt aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa
     8941 tagctgtacg tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg
     9001 acggtgcaaa agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg
     9061 gatcgaataa ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg
     9121 atgacgctaa ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag
     9181 gtcaagtaat agcgaatatg acttatgaag aagctttgaa aaaatatggg aaagcgatca
     9241 cgctaccaaa attcaaagaa ccagattggt caacttcacc ttttaacgga atatatcaag
     9301 gtcagtgtac agagttgacg tgggcttata tgagccaatt atacgatggg aatcaaccga
     9361 ctactggaaa tggtggttat gtatgggaaa gctacaaatc ggcaggagca aaaataacga
     9421 acaaaccaat cgtaggttat gggtttagtg cttcggatgg atggttatgg gctgttggag
     9481 atgcagggca tactggtgta gttatagcgg tgtttgaaga tggatcattt ctatgtgcta
     9541 actttaatgt tccaccaacc caagctccaa caaggggagt ttcggtaacg ttgattgatg
     9601 gagtggacgg atcagataat gtgcatttct ttagcggtgt aggaaaagct aaatttgaaa
     9661 tcgaaaaata aattggaatg aaggacgata tagatgaata gtaaagtgaa agatacaatt
     9721 atcatttctt tgctttctgg tatgttatta gcactgcttg ttggaatcct attcttcaat
     9781 aagcagaatc aggacataga agctgaaaaa cacagtgtgc atattgaaga taagctgaca
     9841 ttctttaaag aggttaaacc aaatgaggtg gatggattac ggaaagattt agaaaatgat
     9901 attcaagaaa tgttgaatgg atcagttcaa aataagtttg cagatcaatc aggtgaagaa
     9961 tttttgagaa aactatttat tgtaacaggt aaaccaccaa ttactgataa gagtagtgag
    10021 aaagaaaaaa tcgagtacta taagcctttt acttttaagc taacaaactt tggaattaag
    10081 acatctagta gtgggttgta tgaagcattg accgattttg aagttcaata caatggaaaa
    10141 aaagtgtatg ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt
    10201 gctttttatg caaaatcatg aaatcactac tgctaaaaag gcgatcggaa tactttccat
    10261 aattgtgatc ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt
    10321 caaagaattg gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt
    10381 gaaagctatg caggaagctc aatacaaaga attgggaaat gctgaaatta aggaacaaag
    10441 taggaaattc tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat
    10501 gaaggaaata caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatttctgg
    10561 cacgatggta ggcactggta atagtccaat atctagctat actgctgatt actatacaac
    10621 aaatcaaaaa ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc
    10681 aacaacaaca atttggcgtg gagtaacaag cataggagaa aatcaattgt tttatttgga
    10741 ggatttagat agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt
    10801 tagctctttg tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa
    10861 cacaaacaga aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat
    10921 ttagattata ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa
    10981 agtcaaaagt aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg
    11041 tttatttgac ataagaaaat ggtctagatg ataattgtaa tatagaattg tagacttaaa
    11101 taaagcataa aatacctttt atgataaggt atagctctta attttaagca gttttagata
    11161 taagattgaa agtaaagtgg gggagaataa taatggcaga tcataaatta gaaaagttat
    11221 tagaagaaac gtggaatcca aaagaattta gtgaattttt tatggagaat tttgaaactg
    11281 acttagcagt gattgttaaa gatgctctta gagagcaagg gtatcctgaa actgcaaact
    11341 atataaacat taattttact ttgtatactg aaaataaagg aacttgggat ttttgggcta
    11401 ctttagcaaa taaggagtta agtgataaat cagacacagg aattcgtaac ttttttgaat
    11461 caaacagaga tgattatatg tatgctaaca atcaaaataa attgaacttt agagtagagt
    11521 ttgatgaaac acctgaagaa tttatagaaa gacaaccgcc taaagaaaat gtagcaaagg
    11581 tattggaaga tcgttggaat tcagatgaaa tagtttcaac aattagcgaa ctaggcggac
    11641 aatatgaacc gctagtagaa gcggttagag aagagctaag actgaataag tttcctgatg
    11701 ttcaaaatat tgatgtttct caaattgaaa taaacgtcaa gatcacgaat aaacttgatt
    11761 atggtagttg ggcagatata gcattagaaa aatacatcta ctcaacgtta aaagagttta
    11821 tcgaaaatag aatggatatc atgtatctac agcatccaca gtatttaaac tttggtgttg
    11881 aaatagctac acctcttgaa gaatggaaaa tggagcaagg gcttgactag cctttgctcc
    11941 atttttttat tttacaaatg aatacagttc gtcgttttta ccaagaatga ttgtttgccc
    12001 aactttgaga ttgtcaggtg atccttcagc ggtttgtaag gagaacgttc cattgaactt
    12061 atcggatacc gttataggta gctttaattc tgatccttcc tcatctttga gaattgctgt
    12121 ttttcgttca tcaaaataaa cagtctttat taaataattt ttcgtagtga tctcagcaga
    12181 tttgtatttt tttgtagtag taccagtttc tttagaatta gaggtatttt ctacgactat
    12241 tccatctttg gcatttgagc ttgtgtttat ttgattgaat attttcagcg acaagataac
    12301 gctaagaata gtcaatatag aaacaagtgt aattaatact gataatttcc tttttttgtt
    12361 cattttttct taacatcctt ttttgttttg attagttaga tattccttat ctaacaagcc
    12421 tttttaggct attaaatgaa gataaacccc aaaaaatatt gcaattatga cagtgcaatg
    12481 gtactattat tatacaataa attttgcgaa aggggtaggt ctaaatcgca aacaaaaaga
    12541 aattgaacct agacaaatac aacttgtata ccgcaaggga agcaagtttg attttgggta
    12601 aaggtagaaa ttatgttaca aacataatca actattatcc aaatcgtttg cctaaaggtt
    12661 cgttcaagaa agtgggtgac aaaacctata ttataacgga agaaggaata gaagcattgc
    12721 gatcacaaac agaaaataaa aacaaaaaga aggaatgaat atgaagagaa attcgcttgg
    12781 tatggtttta ttggcatcag ctgtacttgc ggggttatca gtaccagtaa cctcacatgc
    12841 tgatgaatgg actgcaaaca caccggatca aatccaaatt acaacaggac aatcaagtta
    12901 tgcgttcaaa aaaggagata ctatttggtc aactgtagag aagcttaaaa gcttaggtta
    12961 caagactact gttgctgagt ttgccgaatt gaacaatgtt gatttggcaa aaggagaaca
    13021 acggactatc ccgattggac gaactttcaa catcagtaaa gatacattaa cagttaagga
    13081 agcagatggc gaagtagtta gtcaaactgt tcaaaaagat aaacctgatc ctattgaaaa
    13141 tacggagcaa gagaacaaaa aatccgagac tacaaaagaa tcggaaccaa cagcaaacac
    13201 ttctgataag acagaaacaa gtgcttcaag tgttgatgga gcaagttcat ctaatacaaa
    13261 taattctaca agtgagaaag aagcatcaca gggaacaagt gaaagtagca ctacggaagc
    13321 tgatactgga acatcaacaa ataactcaca atcaacatca aatgatgcaa atgaatcaac
    13381 cagtacatct tctagtgatc aatcacaaac aggttcgagt gatgaaactg acagttctac
    13441 ttcaccaagt aaacccgaac aaccaactaa tgttgtgcta ggcgttccat ttattagtca
    13501 aggtaacaca atgctttgcg aaggaacatc tttgctagaa gcgttacatt ataaaggtgt
    13561 tgcaacaaat caagatttaa tgacttttgt taaatcaatg ccactatctc ctgacaataa
    13621 tccaaataat ggtttttctg gtgaatggag acacaatgtg gatggaactt atcaaggtat
    13681 gaatccggct cctgtagttt catggggtgt tgctaatggc ggaaatgttg tagatatttc
    13741 tggttcatcg gtacaaggat taaaagatga gattgtaaat ggaaatcctg tagtagcgtg
    13801 ggtaacttat gcctttgaac ctgctcaaca taaaccaatg ttttggggcg atgctatttg
    13861 gaatcgacat gtagtagccg ttgatggatt taaagacggt ttttaccatg ttgtcgatcc
    13921 tgtgtttggg gcaagttgga ttaatgcagg atcatttgaa acagcatata acgtatctca
    13981 attagcggtt tcggtacgtt aataaaagat atcgtttata tactaagtag ttatatagtc
    14041 tttatttgtc cttgcttaac tacttagaaa acttgttgct catggtgggt agaactaaaa
    14101 ggggtgatgc tatgaatact agagcgttga aacagcttgc tttcaacgaa aaaaatgatt
    14161 aataggtgtg gtgattttaa cttaggaatg ggcgcgaagg tcattaccac accgacatat
    14221 cgtggctcat tttcttcgcc taactggtat aaagaaggag taacgagaga tgaagcatga
    14281 aaaaacgata attttaaaat caaaaataat ttattgtttt gcgactttag taatgatgtt
    14341 atctacgctt ataatgcctt tttcagcaat aagagctgaa gcagatgtca caaaaccgac
    14401 aagtgcaaaa tttatagaaa ctggcgtaaa aacagacggt tatattaggg ttaatatgcc
    14461 gaatcatcca aatgaatgga tgatttcaag tcaatttaaa gattctcatg gaaatattgg
    14521 atattgtatg gattcagaat tgccatcccc aacaggaagt ggagccggaa gcttgaagta
    14581 caagggagct ggcagtgatg agttctatcg tatgtttaaa ggtggttttc catcaaaaac
    14641 agcaaaggaa ctgggtgcag gaaatgatac agaagcatgg tatgcaacac aattagtttc
    14701 atgggtttta gccggaaact ttaaagtttc acagattgta tggtcacatc caaaccatac
    14761 cgccgcagaa acagctcgtg taaaaaaagc atttgaaaaa atttatgact atgctaagaa
    14821 tggaaaagat acgcccaaca ctgagttttc tatcacagct tctaagacag ctgacgaagg
    14881 aaaatatcac actttcacat ataaaacagc atcaaataag actggtaatg cgaagctcac
    14941 ttttacatca gcaaaaccag ctggtatgaa gatttatgat gcagatggta aagaaattac
    15001 taacaacact gtgaaattaa attcaagttt tactattaaa gtacctgtga ctacaccaag
    15061 tggtactttg tcatttaaag gtactgctaa tgttagcaca accaatcctt ttacttttga
    15121 tggacgaggt gtgtatcaag atgccgtagt tatgatcact acttcagaaa caaaggactc
    15181 taaatctttg agtgctaaat ggacaagagc tgttggtcag gtaacagtta agaaagttga
    15241 tcaagatgga aatgctttaa aaggagcgga gtttactcta actgacaata acaatgctaa
    15301 atctgttaag acaaccggag atgatgggtt agctacgttt gatttgcaac aaggcttaac
    15361 atacagctta gaagaaacaa aagttcctga aggttatcaa ggttcattta aaaaggatgg
    15421 tattactgtt aataccgatg gacaagaatt tagttacact gctacgaaca caattaaaaa
    15481 aggtaaagta aaagttaaaa aggttgacca agatggaaaa ccattagcta atgctgaatt
    15541 taccttgacg gatgattcat cccaaacaca agcagaaaaa acagatgaaa acggacttgc
    15601 aagcttcgat attgtagcaa acaagaccta cagccttgaa gagaccggag taccaactgg
    15661 ttatacaggc gtattcaagc aagaaggaat taccgttaaa gatgatggac aaacttttga
    15721 atatacagct acaaacactt tgaaaaaagg tttagtaaaa gttcataaag tcgatcaaga
    15781 tggaaaacct ttggcaaatg cagagttcac tttaaccgat gaaaaaggtg caacacaagt
    15841 agaaaaaaca gatgaaaatg gacttgcaag cttcaatatt gtagcaaaca agacgtatag
    15901 cctagaagaa accggagtac cagtaggtta cacaggtgca tttaagcaag agggtatcac
    15961 cgttaaagac gatgggcaaa cttttgaata tacagctaca aataatttga aaaaaggtgt
    16021 agtaaaagtt cataaagtcg atcaagatgg aaaaccttta gcagatgcag aattcacctt
    16081 aacggatgaa aaaggtgtaa aagaagttaa aaagacggat gcaactggtc tggctacgtt
    16141 caacattatt gcgaacaaaa cttataactt agaagaaaca aaagtaccaa caggttacac
    16201 aggcgcattc aaacaagaag gaattaccgt caaagatgat ggacaagtct ttgaatatac
    16261 tgctaaaaac actttgaaaa aaggtgtgat caaagttcat aaagtggatg atcaagggaa
    16321 agcattagca aacgcagaat tcactttgac agacgacaaa ggtgtaaaag ttgtacaaaa
    16381 aacggacagc aatggcttgg caacatttaa tattgttgcc aacaaaacgt acaaaatcga
    16441 agaaacacaa aaccctaatg gttatacagg gaaatggtct aaagaaggca tcaccgttgc
    16501 tgacgatgga caaacctttg aatatacagt aattaatcac cttgtacctg aaacagtaag
    16561 aacaggcgga atcggtacat cagccttcct tcttgtattt ctaattgcag gattgagtat
    16621 ggtgctactg tattatcgag aacagcacaa agcaaaataa aaaagtgtta atggaaaaga
    16681 agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa aaaatttttt caaatttaat
    16741 gctatttggt actttgctag gagtgggatt gggcgcaagc tcaatctctt ctagccttgg
    16801 actagggggg caagtagcat atgcacaaca aaactataat tatcaaggaa ctggaacgat
    16861 tcatatccat aaagtaacaa atactaatgg ggatgggaac cctgtctcag atattgataa
    16921 caaagggaaa gaaatagctt taccaaatgg tgtaaaacct cttgaaggcg ttgagttctc
    16981 ttatgtgaaa cttactaaag atgaagctaa atccttggga tggacggata gtgatactac
    17041 aaaaggtcaa catattgatg atgctaaagc acgattatta ctaaacaaaa aatacagtga
    17101 agtaagtaga atgaagactg attcacaagg gaatatagac atacctatac aagtaaatac
    17161 agacgattta acagataact tgtatttgat tgttgaaaca ggaacagtca aaggtgtagt
    17221 tgaagaagcg tatcctatga ttgttaacgt acctgaacta aacggatcag atgatcaata
    17281 ctttaattat gatgtacatg tctatcctaa aaactatttc caattaaact cagcagagct
    17341 tgataaaaaa gacgaagatg gaaaggctct tgccggaact gtttttgaat tgcgaaaaga
    17401 tgatggatct gatcaggttg ttggaactta taaaacagac agcacaggta aattaactgt
    17461 agatggtttg gaattaggtg actattattt cctagaaacc gatttgggaa cagatgctaa
    17521 taaaacaacc tatttactag atggtaaaaa attcaaattt agcgttaaac gtggggagac
    17581 tgctaaagta gttcttgatg ttaccaacta caaacgacct gtaattacta aggtaattga
    17641 taaagaaacc gttgatactg gcgaagatgt tcaatataca ttgaaaccat caattcctgg
    17701 taatattggt aattacgaaa aatatgttgt caaagatgtt ctaggtgcag agttaactta
    17761 tgattcatca gaagtgatcg aaggaatgac tttagatact gattacagta tcgaacgtga
    17821 cggacaaacg acaaactatg tctttactga atcaggtatg gataagcttc agaaacaatt
    17881 tgatgcaggg gatcgcttct ttacaatttt gatgaacgct catactaata gtaaagctaa
    17941 aatggataca ccacttaaga atgatgtttc aacgatctac aacaatggat atgaacccga
    18001 agacgaaaca ccagttgatc acgcttatac aatcattggt gatcaccaat tcattaaaac
    18061 agatggagat acaagcaaag cattgagtgg agcaaaattt gtagtgaaga acaaaattga
    18121 tccgaataaa gttacttacc taaaacaaga tccaacaacg aaatctgtta cttggattgc
    18181 agataaatct aaagctactg aattcgtttc cggatcagat ggtaagttcc aaattgaagg
    18241 cttgcaatat acctatgatg tagattttga taaacaaggt aatccaacaa tcacagataa
    18301 aaaaatcaat gattatgctc ttgaagaagt tgttgctcct tcaggatata caccaactag
    18361 aaatgatgtt ccatttgtgg tatcgaatga tgatgcttct agcgaagtaa cagacatcaa
    18421 gaaccttaaa acacctgaaa cagttcgtac aggtggaatt ggaacagtag tatttctagt
    18481 ggttgcaggt gtggcaggaa cagggctata tgttgtctac aaacgagaac aacacaaagc
    18541 ttaatgaaat aaaattccct agagtgtttt tggctctagg gtttcttttt aaaaagtgtg
    18601 tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt tgatcatctt aaaaattggg
    18661 atgttgctat gtttaggagt aggtctatat cctgtaatta ctcaggctta tacctactac
    18721 caaactcaac aaacactaaa ctcctataag gaaaaatatg atgaagcttc tgaaaaggag
    18781 aatgaagaag tcttcaagag atattcagaa gttaatagga aacgaaaaga cacagaacaa
    18841 acctcagtca cgattcctga agataataac aatattccgt caaatggaaa tcttcagaag
    18901 gataaattag gtgaattagt gggtgaaatt cttataccaa agattaatgt ggatttgcca
    18961 atttatgatg gtgtaaacga tttccaacta tcagcaggag caggaatatt aaccggaact
    19021 gattatccaa ctggtgggat tggaaaccat tctgttatat cagctcattc aggcattcct
    19081 aataaaaaat attttacaga tttagagttg ctcaaacttg gtgataaatt ttttattgat
    19141 atttcaggga aaaaacttgc ttatcaagta gatcggataa aaacaattct accaaccgaa
    19201 tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa cacttatgac atgtacacct
    19261 gttggagata attctcatag gttactcgtt agaggggtta gcatacctta ccaaaaaagt
    19321 attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa aggcgcagat gaactggtat
    19381 cctatcgtag ttgtaggggt tttattaata atagctgtga tagtcattat taaaaaaata
    19441 gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata ctactttttt tgactcttat
    19501 ttgcactttg gttttaacaa tttatcctgc aaatcagctc tatcatcaat tcaaaacaaa
    19561 agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa caaactcctt caaaagagga
    19621 agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt aaccaaggat tagacaatac
    19681 tcagaaaaca gataaaggag tagacaatac tcaggaaaca gacggtggat catttgatat
    19741 tcgtgaagct gaaggaaagg tcaatccata cacaggagat gttttttcaa aaaatcaaac
    19801 gaaagatttt gaaccatcta cctattcagg agcggttggt tatgtctata ttccaaaaat
    19861 aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag catttatata gaggtgtggc
    19921 acaaatgagt gctactagtc tacctttatc aggtaaaggt catcaaacag taatagcagg
    19981 tcatcgtggc ggttatacgt caccacaatt tctttatgtt gatcaattag tagccggaga
    20041 ccacatttat atagattatc ttggtgaaaa agcagtttat acggttttcc gaacagacgt
    20101 tattagaaat tacggtcaga attcaacact gactgatatt cctgatttag atatgttaac
    20161 actttatact tgtcatccat atcctcagaa ctacgatcgt ttattagtta gaagtctaag
    20221 agatgaaaaa gcaaaataca tcacgccaca agtaacagtt ggcaataccg catacgctga
    20281 tgccattgat agcgagtccg tttcagataa gacagaaact gttacatctt ctaatgattt
    20341 atttgaagat gttgatcgtg ccaacaagga attagatgga aataatgatc ttaatattcc
    20401 ggaacaaggt agcgtagaga tgcaagacag aacgtttgct gaaaaggcaa gttcaatatt
    20461 tacaggaatt accaataaac atcagaaaat gcttttagtc atttggactg taataggagc
    20521 attagcaagt ctaaaggttc ttattctttt aatcaaagag tttcgaaaag cataatgaag
    20581 attataaaaa ttgatagagt acagtaaaaa gggattgata ctattattga tctctttttt
    20641 attttgaata gagctagatt cacttttaaa taaaatcaaa actgatctta attctaaaga
    20701 aaaagtcaag aaaaaaatac aataaatttt tttatcagaa tatttatcag aatattatta
    20761 gaaaagataa actcaaaagc ttaatttaat agggttattg gttgtattta gaaataatgt
    20821 atggaaaagc tataaagcaa tagataagat gtagaaaatc ttatttgtga atgaaaaaca
    20881 agtcatcgta aaatattaat aggaggtgta gtaatggtgg gagtatcaaa aacagcttta
    20941 gactcattag ctaaactatt tagtagcagg tttgatggtg cagaatttgt tgaatatcta
    21001 agccaaaacg aaagtggaaa aactctgatc gaaacaatga aaaaagacta tttgaatgaa
    21061 aatgactttc cgacatggac gaatattgaa gatgctacat gggatgtagt atgtacgata
    21121 tattctcctt gggtaacgat tgctgatttt gttttagata aaattggagg ggatacatac
    21181 cctgatgcca aatctcttgc tacaagtgac tttaaaacgt tctatggtta tctaataggt
    21241 ctaataggca atggaataga tttgaaagtt acgccaaaac aaacacctga agaaattgaa
    21301 aaagaaaaag gagaggttca aatggcttat gcaaatgaca gagaaaaagt aaaattattg
    21361 gaaggtaaat tatctgaaga agaacttgga aaaatggtgc aagaaatcaa tgaagacagc
    21421 aactattttc ctgaattgaa ggtatatcaa aatgacgaag atttctttaa tgaaatgttt
    21481 agtaatagtc catgggaagc agtaagtaaa acaaatttag gagaatggaa ctttcaagat
    21541 gatctagttg catttagcaa tgatgggtat ttacgatcat attcagaaag tgagtataga
    21601 gctgaattaa aaacgtatga aacggatatt ctgatgagct atttaaatct attagagagt
    21661 ggtcagggag agttaagaga taaagttgaa gaactattaa aagaagagct atagggatta
    21721 tttcaacata aacacaagca gaaaactacc aaacaaaacg taccatgcaa aaagtcggtt
    21781 aaaccggctt tttgctctat tatgagagga gatcaaacca tgagtgcaac aaccgatatg
    21841 gctttagaag cagtaataaa agataataca ggaaatgtcg aaaatgtaga agactttagc
    21901 gatattgcaa tcaatctttc agcatggatt tcagctgtag gagaagaaaa tatcgaattt
    21961 gagatcaaga atatggatga cttaaaagca aaatttcctt ttattgcatc gaaactaagt
    22021 caagttgccg aggataaatc gtggaatgaa ttagttagtt tttccgaaaa aatttatgat
    22081 attacgcaag aagatgagtg tgtttcatgg gaaatgaata atcaatacaa gaaaattgac
    22141 gctttcgtca aatcacgtat agaaggaaca gcttttgagt ttgaaggtat agataccgca
    22201 gataattggt taaataaaga atcctttatt aaaagtatag cggatgatct tgaaaaacaa
    22261 tggtctaagg aagactatgc agagtaccta aaaacagaaa gagatacaga agtgaaagaa
    22321 aaactcactg aggtatgttt ggatgaacat attgatccca atatcgtaaa acaagaagac
    22381 tatgaagtgg ttattgataa agttcctact gattttgagg aagttgtaaa gtatatagtt
    22441 gaagaagcat tatcgcaata ctcttcagat gaggaagtgg acgaagaaac tgttcgtacc
    22501 agagtttggc aactggtttt tgatgacaaa gattataaat atttgagtga tcaaaactcg
    22561 gttaattgga gtgtacgatt attaattgat caaaatgata ttgaatagag ctgaaaagct
    22621 ctattcagat tgtagacaaa aaggagttgg taattaagcc actccttttt gttagtcttt
    22681 gcataatttt gaaataaaaa gccataggct tttttgtttt ccatagccat gtggctagtt
    22741 tttttaaatt catggcagca aacgtaagca tcgtgtgcgt ggcgactttt tcaagacctc
    22801 gatatttcgt ccaacgcata ccatgctttt cttttgcatc tgcaaatatt cgttcaatgg
    22861 tttgttttct ctttttgtaa atttctctat tcaacgccgt atggcgttgg tgttcaagtt
    22921 catccataag atcagcccaa atatgtcgag tcactatttt ttgatgtttt gtactcaaag
    22981 tacaattttc aagtaatgga caactcacac agaattttgg atttgattta taggttcgat
    23041 aaccatcacg agtgatagtt gaaaataaca acggttgatt attgggacat aaatagcagt
    23101 caaaataggc atcataaaca aagtcttttt tacgaaacat tcctttcact ccatgaggtg
    23161 cagtatatgg aagaatcggg tagagtttca agtgtgacag aaaatgaaca aatttaggcg
    23221 tcttatatcc tgcatcagca accacacgct ttatatttgg aaaagctctt ttcgactgtt
    23281 taaccagatc gattgcgact tggctgtcat gcacatttcc tggtgtgatt aatgtagata
    23341 agataaaacc gttatcttca caggaagtat gtgcagaata agcaaattgt ttttctcgtt
    23401 cacctttaac atagtaacca ctttctggat ctgaatgact tattttttga agactcaact
    23461 cactttctgt gtcccatgta aaaggacgtt ttcctacagc aatacgttct ttatttattt
    23521 catcttctaa ttcatcttga taagcttttg cttgaccgta ggtcatctct ttcgtgaact
    23581 tatgtttatt ggcatttgct ttgatatgag tagaatcaat ataaaggttg tcttccgtaa
    23641 cgaagccagc ttttacagct tgttctagaa tatgagagaa aatatcttca aataaagttg
    23701 tttctctaaa tcttcgaaca tagtttttac caaaagtaga gaagtgtggg ataggatcct
    23761 cgaatgaata acctaaaaac caacggtaag caacatttgt ttcaacgtct ttaattgtct
    23821 gtctcatcga acggatacca aataaatatt ggataaatac taatttaatt aagaccactg
    23881 gatcaatact gggacgacca taggttgaat acgtagattc tacaatggga taaatgaaat
    23941 caaactgaat cgcagcatca atttttcgaa ctaagtgatc tttaggtaca agatcatcaa
    24001 ttgttaacat agcaaattga tttctacctt caatcgattg ttttctcatc attagaatca
    24061 ctcccagaat agtttataca attagtataa aacaaaaaac tgcaaacaac actcc
//
LOCUS       DAXWLB010000027        23375 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXWLB010000027 DAXWLB010000000
VERSION     DAXWLB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.027"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            21..1448
                     /locus_tag="ACGE4H_000827"
     CDS             21..1448
                     /locus_tag="ACGE4H_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364265.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="ACGE4H_000828"
     CDS             complement(1540..1905)
                     /locus_tag="ACGE4H_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8364266.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="ACGE4H_000829"
     CDS             complement(1902..2267)
                     /locus_tag="ACGE4H_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFF8364267.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="ACGE4H_000830"
     CDS             complement(2459..2707)
                     /locus_tag="ACGE4H_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364268.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="ACGE4H_000831"
     CDS             2706..2834
                     /locus_tag="ACGE4H_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFF8364269.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="ACGE4H_000832"
     CDS             2891..4585
                     /locus_tag="ACGE4H_000832"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFF8364270.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4685..4981
                     /locus_tag="ACGE4H_000833"
     CDS             4685..4981
                     /locus_tag="ACGE4H_000833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364271.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5359..6492
                     /locus_tag="ACGE4H_000834"
     CDS             5359..6492
                     /locus_tag="ACGE4H_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFF8364272.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6523..6843
                     /locus_tag="ACGE4H_000835"
     CDS             6523..6843
                     /locus_tag="ACGE4H_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFF8364273.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6872..7336)
                     /locus_tag="ACGE4H_000836"
     CDS             complement(6872..7336)
                     /locus_tag="ACGE4H_000836"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364274.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7467..8720)
                     /locus_tag="ACGE4H_000837"
     CDS             complement(7467..8720)
                     /locus_tag="ACGE4H_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8364275.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8749..9066)
                     /locus_tag="ACGE4H_000838"
     CDS             complement(8749..9066)
                     /locus_tag="ACGE4H_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8364276.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9092..9412)
                     /locus_tag="ACGE4H_000839"
     CDS             complement(9092..9412)
                     /locus_tag="ACGE4H_000839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8364277.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9876..11291)
                     /locus_tag="ACGE4H_000840"
     CDS             complement(9876..11291)
                     /locus_tag="ACGE4H_000840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8364278.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11308..12624)
                     /locus_tag="ACGE4H_000841"
     CDS             complement(11308..12624)
                     /locus_tag="ACGE4H_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8364279.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12774..13472)
                     /locus_tag="ACGE4H_000842"
     CDS             complement(12774..13472)
                     /locus_tag="ACGE4H_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFF8364280.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13761..14754)
                     /locus_tag="ACGE4H_000843"
                     /pseudo
     CDS             complement(13761..14754)
                     /locus_tag="ACGE4H_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14835..15668)
                     /gene="msrA"
                     /locus_tag="ACGE4H_000844"
     CDS             complement(14835..15668)
                     /gene="msrA"
                     /locus_tag="ACGE4H_000844"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8364281.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDTDYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15902..16645)
                     /gene="truA"
                     /locus_tag="ACGE4H_000845"
     CDS             complement(15902..16645)
                     /gene="truA"
                     /locus_tag="ACGE4H_000845"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFF8364282.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16836..17471)
                     /locus_tag="ACGE4H_000846"
     CDS             complement(16836..17471)
                     /locus_tag="ACGE4H_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8364283.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17618..18838)
                     /gene="sstT"
                     /locus_tag="ACGE4H_000847"
     CDS             complement(17618..18838)
                     /gene="sstT"
                     /locus_tag="ACGE4H_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFF8364284.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            complement(19180..19857)
                     /locus_tag="ACGE4H_000848"
     CDS             complement(19180..19857)
                     /locus_tag="ACGE4H_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFF8364285.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20108..20650)
                     /locus_tag="ACGE4H_000849"
     CDS             complement(20108..20650)
                     /locus_tag="ACGE4H_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFF8364286.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20655..20882)
                     /locus_tag="ACGE4H_000850"
     CDS             complement(20655..20882)
                     /locus_tag="ACGE4H_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFF8364287.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(20961..22841)
                     /locus_tag="ACGE4H_000851"
     CDS             complement(20961..22841)
                     /locus_tag="ACGE4H_000851"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8364288.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23142..>23375
                     /locus_tag="ACGE4H_000852"
     CDS             23142..>23375
                     /locus_tag="ACGE4H_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8364289.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKK"
BASE COUNT         7243 a         4808 c         3954 g         7370 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
     4741 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
     4801 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
     4861 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
     4921 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
     4981 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
     5041 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
     5101 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
     5161 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
     5221 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
     5281 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
     5341 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
     5401 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
     5461 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
     5521 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
     5581 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
     5641 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
     5701 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
     5761 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
     5821 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
     5881 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
     5941 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
     6001 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
     6061 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
     6121 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
     6181 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
     6241 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
     6301 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
     6361 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
     6421 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
     6481 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
     6541 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
     6601 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
     6661 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
     6721 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
     6781 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
     6841 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
     6901 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
     6961 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
     7021 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
     7081 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
     7141 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
     7201 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
     7261 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
     7321 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
     7381 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
     7441 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
     7501 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
     7561 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
     7621 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
     7681 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
     7741 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
     7801 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
     7861 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
     7921 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
     7981 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
     8041 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
     8101 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
     8161 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
     8221 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
     8281 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
     8341 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
     8401 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
     8461 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
     8521 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
     8581 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
     8641 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
     8701 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
     8761 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
     8821 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
     8881 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
     8941 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
     9001 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
     9061 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
     9121 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
     9181 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
     9241 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
     9301 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
     9361 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
     9421 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
     9481 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
     9541 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
     9601 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
     9661 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
     9721 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
     9781 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
     9841 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
     9901 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
     9961 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    10021 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    10081 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    10141 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    10201 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    10261 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    10321 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    10381 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    10441 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    10501 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    10561 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    10621 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    10681 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    10741 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    10801 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    10861 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    10921 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    10981 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    11041 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    11101 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    11161 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    11221 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    11281 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    11341 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    11401 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    11461 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    11521 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    11581 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    11641 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    11701 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    11761 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    11821 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    11881 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    11941 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    12001 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    12061 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    12121 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    12181 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    12241 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    12301 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    12361 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    12421 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    12481 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    12541 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    12601 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    12661 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    12721 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    12781 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    12841 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    12901 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    12961 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    13021 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    13081 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    13141 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    13201 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    13261 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    13321 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    13381 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    13441 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    13501 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    13561 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    13621 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    13681 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    13741 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    13801 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    13861 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    13921 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    13981 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    14041 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    14101 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    14161 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    14221 atttttttgt atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc
    14281 ttacaagttg catcaacgct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat
    14341 gatgatagaa gatgtcaaca taatctagcc ccattctttg gaaactttgg tcacaacttg
    14401 atatgatgga tttacgagag ccccattccc catatggccc ttcccacata tgataacctg
    14461 ctttgctaga aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc
    14521 caaagttttt ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg
    14581 taattcccat gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat
    14641 caccaaaatt ttgccataat ccgagaggag agataaaatc ggtaatttta aacctgaatg
    14701 accggattta cgatattcca ttttttcata acgtgcacta tctgcaatat acaatttttc
    14761 tcctcctcat caaatctttt taaaacgctt tcttgttttc tatttttagt ttactacatt
    14821 ttaaagaaaa ggagttaatc aaaatgattt tttcgtatat ttttttgcca tttgtttttt
    14881 actcgttgat agatcagaaa ttgattacgt gcttgctgga tttttttata gcgtttcggt
    14941 tgttttttgt aaaattgctg atgatagttt tctgctggcc aaaacgtacc ggctggttcg
    15001 atttttgtga cgattggttg gtggaaagtt cctgaactgt ctagtttctg ttttgattgt
    15061 tccgctaatt ctttttgtcg ttcgtcttga taaaaaatca ctggacgata ttgcttccct
    15121 ctatcttgaa attgtccaaa tgtatctgtg ggatcagtaa tttgccaata gattgtcagc
    15181 aactcgctat aacttatttc tcttgtgtca taaatgatct ccacagcttc cacatgacct
    15241 gtgtaaccac cacttacttg atcataattc ggtttttcaa catgtccgcc cgtgtatcct
    15301 gacaaaacag ataggatccc ttttttcgat tcaaacggct cgaccataca ccagaaacat
    15361 ccccctgcaa atactgcacg ttcttgatag tcagtatctt gcatggcatg cttggaaaag
    15421 tcatattttt gctccttatc aaatccttcc gtaatcaata aataaaagtc cattacatca
    15481 ggcgtcagat tcattcggat agctaatgga cgtagtgtta cttctagttt tgataactgt
    15541 tctttaacgg atatattttc tcttgtgtca ttctttgtct gaatcaatac ttttcgttcc
    15601 caatctcgtg tattcggatt caaaatcaga ttataaagtg tttgaatgaa tatttgttcc
    15661 tcatccatag attctcctcc tttccgaaga agacaagcta gtgactttag ctgtaattta
    15721 tcataaagtg aggaaataat cgattgaatc gtatttattc aaagaagata agcagcttca
    15781 gatgatataa gatgatcttt atcttaaaaa gcatactcta tggtttggtt taactaaaaa
    15841 acgacctctg aaaaaaggga tgttctccaa ttctcagaag tcgagtaaag atgattcttt
    15901 tttatgagcg ataataaact tcatccaaaa acagaccttg tgcagggacg gtttctcctg
    15961 catgttgccg cactttgctt tcaaatattt catcgattgt ttctatcggc atggcacctg
    16021 tgccgatttc tagtaatgtc cccatgataa tccgcaccat tttatataag aatccgtctc
    16081 cgacaaaagt aaaatgaagc atttctccct ttcgttcgat agataattca tcaatcgtac
    16141 gcactgtgga tttttttgat tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca
    16201 ctaatttttc gcaagctgca ttcagcttgt ccatatccaa ctcttgaggg aaataaaagc
    16261 tgtggtttcg ctcaaatgct gaaggaatca cgttgttcca aacataatag ctgtattttt
    16321 tgcccaccgc attatatctt gaatggaagc gctcaggcat ttcttcgatt cttttgatga
    16381 caatatcccg cggaaggtaa cgattcataa agtcgatcat ttctgccagt ccaatttctg
    16441 tgtttgtttt aaaatttgct acttgtcctc gcgcatgggt gccagcatct gttcttcctg
    16501 atccaataat ttctatctct tcatttgtca tctgatgcaa tactttttcg atttttcctt
    16561 gaatggtttt atcgtcatct ccaagtcttt gccatcctga atatcttttt ccgtcatatt
    16621 cgattgtcaa cttgatgttt ctcatatttt agtggctcct ctgtcttcca tttggtcttc
    16681 tctctacctt tttaagattg tagcacaaaa tatagctcct tgcttctcta agacagaagc
    16741 tatacaagaa aactgtggaa atatatattt cagaaaaaca aaaaacagcc accggatgaa
    16801 gtatccgctg actgttcaat ttgacggctc aagttttact gtttgagatg caagatactc
    16861 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa acctatttta
    16921 ttcagtaatg gccaagaatt tatagaagga attcttgcca gtccataaat cgccgcaaaa
    16981 agcaaactga tcacagacag tccaaggtat aagtggctta cgtccatttg cccatttttt
    17041 cgatagatca agaaaagaag aactggaaac aataaaaaac cactgtaacc taaacctgat
    17101 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
    17161 aaacctgtaa agatacaatc acctatacca tagaacgcga tggctgctgt aactagggta
    17221 gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa
    17281 aagatccctg aaaccacaga ccaaataaga aacgcttttc ttacaggact ggaaacctct
    17341 ccaaatacac tgatcacgct cttcatttga tctaattcag gataatacaa tcctaatacg
    17401 tacggagtca aaaaatcact cagcacgcct aataataaaa agaaaaaacc aaatttacgt
    17461 aaaaaatcca tgatacgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
    17521 gactttatct tgatttaaca gaaacctcta tttacacgaa catctatata actcattaaa
    17581 aactcgaatc attttaaaaa tactaaatat ctaacgttca acgttcactt aatccatcta
    17641 aacaatcttt caatttgatt ttttccccac ttcttttacg cgcgcctagc tctcctattg
    17701 cagtaaacaa cagatcactt gacgaattta aggcagtttc cattgaatcc tggataacac
    17761 cgataataaa tccaatacca acgatttgca tcgcgacatc attagagata ccaaacaaac
    17821 tacatgctaa tggaatcaat aacagtgaac cgcccgcaac acctgaagct ccacaagcag
    17881 ctaaagcaga caaaatacat aaaaggactt tcaaaacaaa tggtgcttca atccctaaag
    17941 aatgtacagc agtcaacgtc atcaaggaaa tggtaatcgc ggcaccgccc atattgatcg
    18001 tagcacctaa cgggatcgat atggaataag actcttttgt caatttcaat ctttcagcca
    18061 acatcatgtt tatcgggata tttgcggcag aacttcgggt aaagaaagcg ggaagtgcgc
    18121 tttctttcaa acaaaagaaa atcaatggat agggattttc tttaataaac aaaaacgtga
    18181 ggaatgggta cacaactaat gcgacaaaca gcatcgtacc gatcaaaaca agtaaaagct
    18241 gcagatactc tgccaatcca gcgattccag tcgtagcaat tgattgataa accaatccta
    18301 aaatccctat cggagcaaat gcaataatca cttgtacgac ttgtgacaac gctatagata
    18361 tctgatccac caactctttt gtcggttctg aacgtgctcg aaaagctagt cccaacaata
    18421 cagcccagaa taaaacgcca aggtaattcg catcaataag agattgaaca ggattttgtg
    18481 tgacattcgt caacatagta gataaaacac tttccaaact ttgcggtgct ttttcagata
    18541 ctgtttcttg taaagtaata ggaacagtaa aaattgaact tacaatgact gctacaagtg
    18601 cagaaagcaa tgttcccact aaatataaga tcaaaatagg cttcacgaat gtcttattcc
    18661 caattttatg cttggcaatt gacgccatcg tcaagaaaaa aaccagaata ggtgcgattg
    18721 ccttcaagca actgacaaac aacgtcccca atattgaaat aaatgtccac gtggggaaaa
    18781 agataccaaa caatattccg actaaaatac caacaacaat acgttgaact aacgacatat
    18841 ccaaaaactt ctctaacaaa tgaccaccct ttcacaattt taacaatcat tgtatcattt
    18901 tatctaaaga aactaaaggt gcaattttct aaaaattcta ataatacaat tggcaattca
    18961 tgctcttgca acattcaatt ttagtattgg agggtttatt caatctctaa agaagtcgag
    19021 agaaaactat ttatcgttat tattatagtt atccaagtaa aacgtaacta agatgaagga
    19081 cgaaactaga aatttcatcc ttcatcttaa ttcttagaca tagcaatcaa ttaatatttt
    19141 ccactgagtc tcttctttct ctcactctca ctggtttact tacccataaa cgatttgatt
    19201 aaagccattt ttcaattcat tattcgctgt ctcgttaaag taaagataat tatcgggatt
    19261 cccctgattt tgttgtgtca ccaatacttt tggctgattt tgctgaaacc cgaatagata
    19321 gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa tagacggctg tgatttgata
    19381 gtctgcttga cctgtcccac tttctgacca ttctgcagaa acaggcactc caccaatagc
    19441 catcttccac tccccgttca aaataacttg agggacttta agaccatata agtccacact
    19501 catttgattc gtatagcttt tgtattgttg tcccattgtt tggccccatg tagccataaa
    19561 tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt tgttcgactt gttcgctgct
    19621 ttctgaagat gaagcctcta cagaagaact tgaagtagat gtttgagttg tttcagtcgt
    19681 tttggaagac tctgacgaag tagaggtatc tgctgtttcc gtacttgctg cagttggctt
    19741 gctctctgtg ctagttgttg tagtagttgc aattgttgct ttcgctgttt cttttgttga
    19801 ttcttttttc gattcattcc cgttggtaca accagctaac aaaaaagcta gaccaattgt
    19861 tactaaaatc ctttttttaa attaaaaaac tcctttcgat tactcccact tctttattct
    19921 atcaaataag caatgtgatt ttattgattt tttgtatttt ctcatccatc ttttaaacct
    19981 tttaaaaact aagaagcacc cgctgatgtt gtctgtaata catcagcggg tgcttctttt
    20041 attttaatct tcgcgatttt ccaatttacc tcacgcttta tatttgtatt tgaagaaaca
    20101 tagactttta gtcatcatcg tcttcctcat ccaaatcttg tcggacagtt tttccttgga
    20161 gtctttgtac ttggtagatg atttctttat tgaaacttaa aagttcaaca agcaattgtt
    20221 tcaagccata tttttcttct ttttcagcta atttgattgc ccgtgtgata aagacatttt
    20281 ggaaatcaaa atcttttgca aagttttcca acattgttga ggctggtaaa tatttgtttt
    20341 gaccatgttc gtaaattttt ccataacgca tgacttcttc taaagtagac ggaacgattt
    20401 cattttcaat cactaactca tgagagattt tattgaacca tctcgcttct tgatcagcat
    20461 attctgaaaa aagggtactt aattcttgcg aagatgtacc caaaacatac aattgtgctt
    20521 ggatcaattt catttcttct aatttaaaat ttgccaatac atgccccacc atggcgccag
    20581 cagttggttt atgatgatcg atatctgctt gttttgtttc ttgagcaaat ttttcttcca
    20641 ctatattcac tgatttacgc ctcttttact tttgttgatt ggacttcata acctaatttt
    20701 tccacaactg cagctaattg ttcagggtgg acttcatctg gatcaaagtt tgctttgatt
    20761 ttgctcgcat taaacaaaac tttcgtattg cttaccccag gctgttgaga aaccgcatgt
    20821 tctatttttt gcatacagct tggacaagct agtgtatcta attgcataat catttttttc
    20881 atcgtagttc ttccttcctt tttcttgata gtcttattct agattacagc ttccataaaa
    20941 aaattgattc ttatcaagtt ttacgatttt tcttctttgc tttcgtgtcc aaaatacaat
    21001 aaacgcatcg cgttaaagat aacgatcaag atactagctt cgtgaacgaa catcccacta
    21061 gccatgtaaa taaacccggc aaataagccg atgagtaaaa ataagacgac accaatggca
    21121 atcactacat tttcacgggt attcaaaaca gttttcttag ccaaacggta agcatgaacc
    21181 aacgatccaa aactagattg catcaagaca acgtcactcg tttcgatagc gacatccgtt
    21241 cctgatccca tagcaatccc gatatcagcc gcagccaaag agggagaatc attgattcca
    21301 tctccaatga aagcaacgtg caatccttgt tcttggtatt ttttcacaaa ttgaaccttt
    21361 tcatctggca ataattcagc atggacttca tcaatgccta acatgtcagc tacataatca
    21421 gcagtcatct gattatcacc tgtcagcatc actaaatgtt ttgctcctgc ttgtttcaac
    21481 atagctaatt gttcagcgac ttctggtcga atcacatctg acacaccaat gatttgagtg
    21541 atttttttgt cgattgctac aataatgact gagctgcctc tttgttgtaa ttgatgtaaa
    21601 tcttgtactt gctttggtgt gagagaaagt ttgtgagcgg caatcaattt ggtgttgccc
    21661 gcacaaactg tatgaccatc aatctcagct gtgattcctt gtcccttgat cgtctgatta
    21721 tctttgactg ctaattgttg aaaatcaatg tctttctcct gagcataatg gatcacggcg
    21781 cgccccagag gatgatcgga taacgtttct aatcgagcca ctaaagctaa gctgtcccct
    21841 tcatcctctg tatagttctt gatagtggat acagcagtag ttcctttggt caatgtaccg
    21901 gttttatcaa agacaaaagt atcgattttg gaaaaagtat ccattgcttc accgcctttg
    21961 actaagatgc cccgtttcgc tccattaccg attcctgcta cattcgaaac aggcgcacca
    22021 atgactaaag cgccaggaca accaagaacc aaaacagtga tggctaaacg aaaatcacga
    22081 ctgatcagac caacaagcaa cgcaataagt aaaacggttg gagtataata ctgagaaaat
    22141 tgatcaatga atttttccgc atgtgatttg gtatcttgtg cttcttcgac taattctata
    22201 attttggcaa aggtcgtatc atctccgacc ttagttgctt cgatcgtcac gtagccatct
    22261 gctaaaatcg ttccagaaaa tacgttatct ccactgattt tgacgacttg tcgtgattca
    22321 cccgtcactg cggcttcatc cgcataccct ttcccgtcaa tgactcgtcc gtccacagga
    22381 atagctccac cagtttttac taagacgata tcgccttctt ccacgtcatc gatttccact
    22441 tcttcggtcg ttccatcttc ttggacaacg ctggcagtcg atggggccat ctcggtcaaa
    22501 gccttgattg attcccgcgt tttggtcaat gttttttgtt ccagataact accaaacaaa
    22561 aacaagaatg taacgatagc tgactccttg tattctccga tcataaaggc accaagcaca
    22621 gcaatagtaa cgagtaaatc aattgagata actttgactt ttactgcctg atacgcatgg
    22681 attataatag gtatgaccgc aataatagaa gctatggcta gagcaatgtt atagaacaag
    22741 acaaatccag taaatttccc gataaaacca aaaataatca acacagctga taaggcggtg
    22801 atatgatttc gatgctcttg gatccacttt tgtaatttca tctttctccc tcttttcctt
    22861 ttcattctta cgagactaag tatagatact tatccatcat aaaaaattga ccactatcaa
    22921 gtttattcaa aaaggcggtt ttcttttggt atactgaatt cctacagtca ttaaacgatc
    22981 atatagaaaa aggaagcgcc gaaacgcttc cttaggtagt tctatttttt tattcaaaga
    23041 taagatgctt tgtcatctta cgactgaaga acaacaatca aaaaagggca gcagccactc
    23101 attcacaaga aatatcgttt tttgatatgg gagttgaagc agtggacaaa tatctctact
    23161 tattagctgg gaataagatc caaaaaagct taatggattt tatccaagaa ttggaatgta
    23221 ctttccacaa aaaattcact cattccattc ttttaaaact tttgattcat acagcgtgtc
    23281 taattgagcg tacattgata aacggtcatg aacttaaaat tatatcagaa gatgacacaa
    23341 gaccttctca tgaaacaatt tttcatgtaa aaaag
//
LOCUS       DAXWLB010000028        23238 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXWLB010000028 DAXWLB010000000
VERSION     DAXWLB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.028"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..226
                     /locus_tag="ACGE4H_000853"
     CDS             <1..226
                     /locus_tag="ACGE4H_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364290.1"
                     /translation="VAEVQKLGKSMWTEKPKRRTPRKIAIWHDDRILVTGTAEQLSEI
                     TGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            226..582
                     /locus_tag="ACGE4H_000854"
     CDS             226..582
                     /locus_tag="ACGE4H_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFF8364291.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            542..787
                     /locus_tag="ACGE4H_000855"
     CDS             542..787
                     /locus_tag="ACGE4H_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364292.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            784..1203
                     /locus_tag="ACGE4H_000856"
     CDS             784..1203
                     /locus_tag="ACGE4H_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFF8364293.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            1200..1757
                     /locus_tag="ACGE4H_000857"
     CDS             1200..1757
                     /locus_tag="ACGE4H_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFF8364294.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1754..2050
                     /locus_tag="ACGE4H_000858"
     CDS             1754..2050
                     /locus_tag="ACGE4H_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364295.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            2127..2540
                     /locus_tag="ACGE4H_000859"
     CDS             2127..2540
                     /locus_tag="ACGE4H_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFF8364296.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            2849..3001
                     /locus_tag="ACGE4H_000860"
     CDS             2849..3001
                     /locus_tag="ACGE4H_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364297.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(2998..3273)
                     /locus_tag="ACGE4H_000861"
     CDS             complement(2998..3273)
                     /locus_tag="ACGE4H_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364298.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            3769..3933
                     /locus_tag="ACGE4H_000862"
     CDS             3769..3933
                     /locus_tag="ACGE4H_000862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364299.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            4129..4296
                     /locus_tag="ACGE4H_000863"
     CDS             4129..4296
                     /locus_tag="ACGE4H_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8364300.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            4322..4666
                     /locus_tag="ACGE4H_000864"
     CDS             4322..4666
                     /locus_tag="ACGE4H_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFF8364301.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4671..4952
                     /locus_tag="ACGE4H_000865"
     CDS             4671..4952
                     /locus_tag="ACGE4H_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364302.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            5055..5369
                     /locus_tag="ACGE4H_000866"
     CDS             5055..5369
                     /locus_tag="ACGE4H_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFF8364303.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5347..7041
                     /locus_tag="ACGE4H_000867"
     CDS             5347..7041
                     /locus_tag="ACGE4H_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFF8364304.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7061..8239
                     /locus_tag="ACGE4H_000868"
     CDS             7061..8239
                     /locus_tag="ACGE4H_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFF8364305.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8202..8888
                     /locus_tag="ACGE4H_000869"
     CDS             8202..8888
                     /locus_tag="ACGE4H_000869"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFF8364306.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8888..10048
                     /locus_tag="ACGE4H_000870"
     CDS             8888..10048
                     /locus_tag="ACGE4H_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFF8364307.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10058..10933
                     /locus_tag="ACGE4H_000871"
     CDS             10058..10933
                     /locus_tag="ACGE4H_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364308.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFLEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYRKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10930..11241
                     /locus_tag="ACGE4H_000872"
     CDS             10930..11241
                     /locus_tag="ACGE4H_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364309.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11231..11584
                     /locus_tag="ACGE4H_000873"
     CDS             11231..11584
                     /locus_tag="ACGE4H_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFF8364310.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11574..11975
                     /locus_tag="ACGE4H_000874"
     CDS             11574..11975
                     /locus_tag="ACGE4H_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364311.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            11968..12372
                     /locus_tag="ACGE4H_000875"
     CDS             11968..12372
                     /locus_tag="ACGE4H_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364312.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12384..12992
                     /locus_tag="ACGE4H_000876"
     CDS             12384..12992
                     /locus_tag="ACGE4H_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8364313.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPNNMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            13012..13374
                     /locus_tag="ACGE4H_000877"
     CDS             13012..13374
                     /locus_tag="ACGE4H_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364314.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13377..13559
                     /locus_tag="ACGE4H_000878"
     CDS             13377..13559
                     /locus_tag="ACGE4H_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364315.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13577..16897
                     /locus_tag="ACGE4H_000879"
     CDS             13577..16897
                     /locus_tag="ACGE4H_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8364316.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            16948..17685
                     /locus_tag="ACGE4H_000880"
     CDS             16948..17685
                     /locus_tag="ACGE4H_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFF8364317.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17695..19986
                     /locus_tag="ACGE4H_000881"
     CDS             17695..19986
                     /locus_tag="ACGE4H_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFF8364318.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20010..22232
                     /locus_tag="ACGE4H_000882"
     CDS             20010..22232
                     /locus_tag="ACGE4H_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364319.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVASGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDAPHYLSKTPLGENIANKSVTF
                     PINTSAYKLYEYNTEEDLIIGQTYTITLKGTKPASQTFAVYNYWNINLGSLKPVEGLT
                     DVWSITFTPTTLEPGLPKDLRILQSPKETAGACQIDWLKIEKGDTRTPNIEQYKYRGI
                     GMRDSNNPKDYVWDLAPEYVENNLATDIKVAEITGKANNYTDGKVSQINSQLTASINE
                     VDTTAKGAQTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGT
                     GYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGRKYASWLYTDLYKDGTSVD
                     NLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD
                     "
     gene            22310..22552
                     /locus_tag="ACGE4H_000883"
     CDS             22310..22552
                     /locus_tag="ACGE4H_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFF8364320.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22568..22765
                     /locus_tag="ACGE4H_000884"
     CDS             22568..22765
                     /locus_tag="ACGE4H_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8364321.1"
                     /translation="MVLPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22776..>23238
                     /locus_tag="ACGE4H_000885"
     CDS             22776..>23238
                     /locus_tag="ACGE4H_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8364322.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQP"
BASE COUNT         8206 a         3483 c         4951 g         6598 t
ORIGIN      
        1 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
       61 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
      121 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
      181 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
      241 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca
      301 aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt
      361 agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg
      421 ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat
      481 cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca
      541 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
      601 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      661 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      721 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      781 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      841 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      901 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat
      961 aagaatggtg tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc
     1021 gattacttag ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt
     1081 tgtgccactg ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa
     1141 tacacgtacg aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga
     1201 tgaataaaca ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa
     1261 tagctgcttg ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg
     1321 aaccgcagaa accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc
     1381 cttttacaaa agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt
     1441 atttctgtga ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac
     1501 tcgcttgggc aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg
     1561 ttgagaaaga gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg
     1621 ctttatgggg aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc
     1681 ttttcactga taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag
     1741 aggtggtaga aagatgaaac taaaagacgg attttacgct agcagtcacg gtatcggcgg
     1801 tttaatgcta gatatgccga caaagaaccc taaaacacgc aagaaatcaa aattcaaagt
     1861 cggtgacatg gtccgctgtg aagcagaaga gttcgtttat ccgttcagag gatacgttaa
     1921 aaagatactg tcaaactcag caatcattcg tattgaaaat acaatgaagt gtgatcgttc
     1981 gactgcaaaa cacaagcatt atttagctgt agcgagatta gttgacatgg aagtaatcaa
     2041 gagcaaataa aaaaagccgg atcgctccga ctgattcaat aaatccaaca catttattat
     2101 atcacataaa ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga
     2161 caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc
     2221 gctctttgat agatattaag tcgccgatta taacggatat gccgaaggca ccgaagcatg
     2281 gcaataaggc agaagacgcg attattcaga tgatggatat agaaacggag agagatgcga
     2341 ttctagcggc tttgatggct cttagtctga ttagtcgtca gatactctac tacagcttct
     2401 gtgtgccaga tagcttctca aactacagaa ttagccgtga agtgggttat tcagaaagaa
     2461 gtatacaacg gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa
     2521 aaataattgc ttataaataa tttggcggtt ttttggcgga atgatggcgg tttttagaca
     2581 tttaccagtg ttattatggt agtgtcgaaa gattaggaaa caggatcgac aaaataaacg
     2641 aagggaggca atctccctca tcgttttaaa cttcttctta gatagacagc aactaattat
     2701 gaagaaaagg atgtgaattc aactcctcct aaattcttca tcggttgctg tttattttga
     2761 ttagacagca gcactcacaa aactaaacat gttcttgtcg tctttcaact gctgctgtct
     2821 attaatttat gtattggagg gaagaaaaat gaaattatca gtagaaggca ctccagaaga
     2881 aatacaagaa ttgctccaag ctattggtag tagcaaggag caaaaggtaa gtgtttctaa
     2941 ttcagatatt gacggtattt gcaattgttt aaacagagaa aaaatatcta atccttacta
     3001 agagaaaata tgttcgttaa tgaatttcca attttcctct gtagctctac cttgataatt
     3061 tttttgcagt tcacagatga ataaggaatc gttattatca agtactgtat ttaatttttc
     3121 acacatttga tttggattca aattagtttt aactaaccaa aaagattttt gtagctttat
     3181 atatgcccca aattctttta ttatttcaaa tactttatca tacttttgcc ctgggttatt
     3241 aagatcataa ctaatcatgt agggtttata catgttgtct tgcacatcct tgttttatat
     3301 ttcagcggac cactcgctga taaataaaat tatacgctta gtatttattt tcacaatatt
     3361 aatttatcgt aataactttt gagtaaatag aacaaaaaac ctgcactagt ttccgctagt
     3421 acaggcagtg actatatcgg ttgataatct agcatatatt aaaaataatt gcaagaaaag
     3481 aaatttatag tggtttatac caaattatca aagcaatgtt attttgttgc tgtctattgt
     3541 tttttaatta ttcacacgat aactaaaggt gggtgaagag aaatgattcc attaataatt
     3601 tcaatttttg cgctctgtct taatgtctat atgattggat ttaaaaatgg gcaaaataaa
     3661 aaatagtagc agccaagaat aattttatag tgtcactgtg gcggaaaggg tagacgctaa
     3721 gcatgtgtgc taggtcaatg cttcggcaac catgcaaggt tcgattcctt gccagcgaca
     3781 ttaaatgcct atgacggtta cgactaccga aaaaagatcg ttaagaagct atacggtgct
     3841 acgtacggca atgtagtaag tgtgctatct gtacaccacc aagcttcggt cactgtggcg
     3901 gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt aagttctcgt
     3961 gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg tttaaaaata
     4021 taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt acatattaga
     4081 tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat gtatagacca
     4141 caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg cgaaaaagta
     4201 tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa caatgaagtt
     4261 attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa ggagacggaa
     4321 catgaccgag gaattctata gatggctatt acagttgaca agagaagatc gtttggttaa
     4381 gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac gagatcacta
     4441 tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg ttcatcatat
     4501 aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta tttgtttatg
     4561 tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata aacaagagaa
     4621 gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc atgatcatca
     4681 atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct tatactcagg
     4741 gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg ttatcagata
     4801 actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa gataataacg
     4861 tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat tacattgact
     4921 tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat cgcaaatttt
     4981 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
     5041 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
     5101 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
     5161 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
     5221 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
     5281 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
     5341 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
     5401 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
     5461 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
     5521 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
     5581 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
     5641 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
     5701 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
     5761 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
     5821 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
     5881 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
     5941 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
     6001 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
     6061 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
     6121 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
     6181 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
     6241 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
     6301 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
     6361 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
     6421 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
     6481 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
     6541 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
     6601 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
     6661 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
     6721 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
     6781 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
     6841 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
     6901 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
     6961 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
     7021 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
     7081 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
     7141 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
     7201 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
     7261 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
     7321 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
     7381 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
     7441 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
     7501 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
     7561 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
     7621 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
     7681 gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg tatttcaatt
     7741 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
     7801 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
     7861 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
     7921 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
     7981 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
     8041 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
     8101 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
     8161 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
     8221 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
     8281 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
     8341 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
     8401 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
     8461 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
     8521 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
     8581 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
     8641 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
     8701 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
     8761 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
     8821 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
     8881 taaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
     8941 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
     9001 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
     9061 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
     9121 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
     9181 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
     9241 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
     9301 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
     9361 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
     9421 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
     9481 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
     9541 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
     9601 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
     9661 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
     9721 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
     9781 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
     9841 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
     9901 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
     9961 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    10021 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    10081 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    10141 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta tttttcttga
    10201 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    10261 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    10321 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    10381 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    10441 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    10501 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    10561 taaccgtcct aaagcgaatt atcggaaaaa ggtttatctg gattctaatc ggctatatcg
    10621 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
    10681 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    10741 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    10801 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    10861 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    10921 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    10981 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    11041 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    11101 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    11161 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    11221 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    11281 gattttgacg caaactacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    11341 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    11401 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    11461 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    11521 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    11581 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    11641 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    11701 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    11761 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    11821 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    11881 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    11941 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    12001 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    12061 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    12121 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    12181 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    12241 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    12301 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    12361 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    12421 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    12481 attgtaatgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    12541 cgacagaagt aaaatcaaaa tctaaaccaa acaatatgac ggtaacaatt actgcacatg
    12601 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    12661 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    12721 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    12781 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    12841 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    12901 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    12961 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    13021 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    13081 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgtcg
    13141 atgggaaaag gctctatgga tattacagtt attcaaggtg caaaagccgt atatgtagct
    13201 tatcgtcaag cgaacatggt cgattacatt tcattcgatg aatttataga taaatgggat
    13261 tttgatatgg aggttgcagt agctgtatac agtactatga tgttcaaaca agcacgtgat
    13321 gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    13381 caaagctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    13441 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    13501 acaagagcgc ttatgaagga tggaagaact atgcaataaa tcaatcccag aaaaactaaa
    13561 ggaaaggagg taaaaaatgg ctaaaaagag aacggaagca gaagtaactt tcatagctaa
    13621 cgatgacgga ttgaaatcta cgttaaaaga aatcagtgct gaattaacta aaaatagagc
    13681 agaattaaaa ctagaacaag ctcaattaca acagactggt tctgaatcag acaagttagg
    13741 aagtaaatta tcttctttag aaaagcagta tgaattacaa agtcaaaaag ttgaagtaac
    13801 tagtcaacgt ttagccaatg ccaaaaaata ttatggagaa aattccaccg aagttcagaa
    13861 acttgaaaga gaactgatta atcaacaaac agctcagcaa aggttatcta atgatataga
    13921 taaaacgagt aaggcattgg ctcaatctaa aggtgaattt aaaacgtatg agtcaactat
    13981 gaaagactta gataatgagc aacaacatct aaagtcaagc gcatcattag tagaatccga
    14041 atataaaaaa tggcaagcaa ctgctggcca gtctgctaca gaatctgaaa aattagcaaa
    14101 agcacaagaa tatgtcggca aacagagtga tattgctgaa caaaaaatcg aagtgctaaa
    14161 aaaacaatta gatgccacac aaaaagaatt tggagaaaca tctaccgaag ctttagaaat
    14221 gaaaactaag cttaatgatg cggaaagaga attcgaagaa ttaagtaatg ctgctaaaaa
    14281 cgttgatacc tccacagtag atgatatcgg caaaaaactt gatatgggta atttaatgga
    14341 agctactgat cacttgtcag tgatcggaga taagcttatt gatgtaggta gtaagtctat
    14401 tgaagcagct ggaaaagcac aagctatgca ggcccaattt aaacaagtct ttggctcttt
    14461 agaaggggaa gcacaggacg ccgttgaggg aatggctgaa gaatttggaa tgttaccaaa
    14521 tacgatcaag cctgttttta cacaatatac gtcaatgttt aaaggacttg gatatgatac
    14581 caaagaagct atggagttag ctggtgatag tactcagtta gcagcagatg cagcagcttt
    14641 ttacgataag tctatggatg atgctagtga atctcttaat tcatttataa aagggaacta
    14701 cgaaggtggg gagcaaatag gtttatttgc taatgatact caaatggcag cttatgctgt
    14761 taagcataat ttgataccag cgactgaagg agcaaaaaaa gccagtgaag agtcattgtt
    14821 agctgttgaa aaagcacaat ctaagtatgc tgatgctatt aagaaacatg gtgaaggatc
    14881 tttagaagca agagaggctg ctttaaaact taaagatgcg caagataaaa taaatgaaga
    14941 attaggccca caaacgcaaa aatggtctga tttagatgag gctaccaagc aagcagttcg
    15001 agttcaatat gctgaagata tgcaaaaatt agccggtgct acaggtcagg ctagtaggga
    15061 gtctgatggt ttagaaaacc aaatgacaag agcaaagcaa gcgttagaag atttttatgc
    15121 ctcattgggt gaagatatac tgcctgtgtt tatcaaaggg ttgcaagcag gagcgaaagc
    15181 tttgcaagga ttagccgaat ggtggagtaa acttgatggg ccaatgaaaa atttcatttt
    15241 agctcttgga ggaattctag cattattaag cacattagct cctgttataa ccgcagttgt
    15301 tacgatagtt ggcacatttg gttctacagt tttgctgcca ataataggaa ttatagcggg
    15361 cgttgcagct gtaatagcga ttgtaattac agcgtttcaa aactggggcg caatcactga
    15421 ctggtttagt gatttatgga aaaaatttac cgattggtta ggtgatacct gggaaagtat
    15481 aaaagaaagt gcctcatcag tttgggatgg agtcaaagaa acctggtctg gatttgtaga
    15541 ttgggttcaa gaaatttggc aaggagtttc tgattggttt ggagagttat ggagcggatt
    15601 agttgaagga gcttccaaca tctggcaagg agtccaagag acttggcaag cattcgttga
    15661 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    15721 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    15781 tattatcgtg ggtatttttg atgtagctgg acagttatta ggcgcagctt ggcaagctgt
    15841 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    15901 catcgcttgg aatggaattg taactacatt ccatactata tttgatccag tagtggcatg
    15961 gtggaatggt atatggacat ctattagtac tacggcttca aatatttgga actcaattag
    16021 tgcaacagct tctagtattt ggaatagtat caagaataca atcactagct tggtacaagc
    16081 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    16141 gaattcaatc agctctacag catcaaatat ctggagtagc gtgactagca gtataagcaa
    16201 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    16261 cagcggaatt tggaacggta tcaaaaacac tgctttgaat ctttggaatg gaattacaag
    16321 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    16381 aacaggtatt gtttccgaca tattcaataa tgttaaaagc acgatagcta atatatggga
    16441 aggtatcaaa aagactgtta gcgctccgat tgattggatt agagataaaa tcagtagtgt
    16501 ctttgataat ttgaatattt cgataccaca tattccgtta ccacatttta aattgagcgg
    16561 agaattcaat ccattgaagg ggaaaatccc aacattgggt gttgattggt atgcgaaagg
    16621 tagtgtgttt aattctccaa atattatcgg tgtcggtgaa gcaggacctg aagcagtttt
    16681 acctttgaaa agatctgtgc tgcaagaaat tggggatcgt atcttgagta gcacatcagt
    16741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    16801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    16861 aaaagttcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    16921 ttttagtttt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    16981 tttcctgatt atttgcataa attggcttta gcggaaagac caaccatacc ttctccaaaa
    17041 agacagagag ttgaaacttc tgttttagga cggctaggtg gcttagtaca agattactcg
    17101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    17161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    17221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    17281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    17341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    17401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    17461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    17521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    17581 aacaaaatga agaccatgca atatccgaca ttgcaagtgg atatcaatca ttttaattgc
    17641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    17701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    17761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    17821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    17881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    17941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    18001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    18061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    18121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    18181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    18241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    18301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    18361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    18421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    18481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    18541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    18601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    18661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    18721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    18781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaacggt
    18841 ggcgaaatca gtgatttact caatgatatt gtagatcatc aaactgatat gattactggt
    18901 caaaatggtg gttatgtttt attagatcct aaagaagcgc ctagtcgtat tttgattatg
    18961 gacacaccag ataagaatac cgcaaggaat gttttacaaa tcaacaatgc tggtattggt
    19021 ttttctaaaa ctggcattaa tggaacatat gaaacggcat ggacgttaga tggcggattc
    19081 aatgcctcgt ttattacggc tggtgaaata gtaggaatta ctattagagg tactacatta
    19141 attagtgatg gtgctgatta tagaacaagt attgctaatg gcaaaatgac ttggtactca
    19201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    19261 ggtattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    19321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    19381 ccaaatttca gatatagcaa tgctagttat gtaacttctg gcgatggtaa ttctttgggt
    19441 atagatggta gtgcgggtaa ctcatgggaa tttaaggtag ctggtaggac tatgaaattt
    19501 actagtgatg gtatgctaac gttaccaggt tgtttttttg gttcatggga agatgggaaa
    19561 cttgctaggt ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    19621 aaaaactcaa ctgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    19681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgacgatgg taaagttcgt
    19741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    19801 acacctacag aactagtttt gtgtgctgtt actcatgaag atattgacca tttcatcatt
    19861 gaaactagta agccaaacgt attagttaga tggaatttag tggcacaccg tctagggtat
    19921 gaagatatta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    19981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    20041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    20101 ttacggtgac tgttaaacca tttttaaatg gcttgcagat ggatacgagt ggcggaacat
    20161 ttactttaaa aggaacaaca ccatctaacc gttatgtaga tagtgttgca actagcgtaa
    20221 ctagtgaaga agtcatattt tctcttgatg gcacatttat gagtgaagca ggatattata
    20281 aacactgcta cgtagaatat agaaaaggta atcaaatttt aacaacgcaa gacatcattt
    20341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatac gtttcgcaat
    20401 tggaagagtt gattcgaaag tataacgaaa cttttgatgc ttttatggct gaaattaaag
    20461 gtagagtgga tagcttaaat caacagatta ctgatttaac tggtcaagct aaaacgctac
    20521 aagacaagtt agatgctctg aaagaagaaa tttctaagtt aggtaactta caagtgatgt
    20581 acagcaacag catcgacttc ggtgactatg attattcggg gaacccgaat ttgttaagta
    20641 agctatcata cgacttaatt gaaaatcaaa atacttcagc tggaacactt tctaagggtg
    20701 aaaactcgtt taaatataat aagatatcag ctgaagtgga aggtggagta gagttatatt
    20761 ataaacgaag aggtatagct aactggttac cctctaataa aacgcttgta atgactgtta
    20821 agcttagagc tggagcggac tatagtccag ttgacggaaa acttatactg attagatata
    20881 ggtatgttgc cagtggaact ggcaagattg ttttagactt acctattaac agtaattcga
    20941 taactcagga atggaaagag tttagtatta ctggaactac tccaacattt agcccacaag
    21001 cataccatcc ttggatacaa tttagggctc aagatggggt acttggggaa atagaaatga
    21061 gctatgacat caaaatcgaa gaaggctcaa cagctacacc ataccagcct aatttgctgg
    21121 acgctccaca ttatttaagt aaaacaccat taggtgaaaa tattgctaat aagtctgtga
    21181 ctttcccaat taacactagc gcgtataaac tatatgaata taatacggaa gaagatctta
    21241 taataggtca aacgtatact attacgctta aaggaacaaa acctgcaagt caaacctttg
    21301 cagtgtataa ttattggaac attaatcttg gaagcttaaa accagttgag ggattgacag
    21361 acgtatggtc tataacattc acaccaacga cacttgagcc aggtttgcct aaagaccttc
    21421 gcattcttca gtcgcctaaa gaaacggccg gcgcatgcca aattgactgg ctcaagattg
    21481 aaaaaggcga cacacgaacc ccgaatattg agcaatataa ataccgagga atcggcatgc
    21541 gagactcaaa taatccaaaa gactatgtct gggatttagc accagaatat gtagaaaaca
    21601 atctggctac agatatcaaa gttgctgaaa ttactggtaa agcaaacaat tataccgatg
    21661 ggaaagtatc gcagattaat tcgcagttga ctgcttcaat taatgaagta gacaccacag
    21721 ctaagggtgc tcaaacaaaa gcgaatgcta atgcgactgc tatagatgaa ttagacaata
    21781 agatcgatga acgcattaat gatacagcta ctaccgcatt ttatggcgca caaggtgaag
    21841 tctctaacaa aacgaaagta gcggagtatg gagtaggaac agggtatact actacagctg
    21901 taggagaaac acatttcgaa cgtcaatcta acggatacgt taaatgctta aaagctggta
    21961 agtattcaat tagtgcgcaa atacgtgtgc aattgggcag gaaatatgct tcatggttat
    22021 atactgattt atataaagat ggaacttctg ttgataattt agtatcttat ggggtcgacg
    22081 ccttgcaaaa tagatttgca gctagcggaa acattgtaac tgatttagaa gtaaacgatg
    22141 tactaaatat gcgtactgaa atcggcgttg cagatggaat tttacaattt acatcttgcc
    22201 gtactttagt cttgactaga ctatctgatt aatacaatat ttataatttt gctatcaaca
    22261 cgctcaaagg agggtgtttt ttattttgca atgaaaggag gctagttggt tgaaagacga
    22321 agcaatacaa gacgtggtag aacgcttagt gcgtattgaa acgaaactgg ataattacga
    22381 attattacgt gaaaaagcgg aaagtgcaaa agatagagcg gatcaggcat attctattgc
    22441 gcttaataat gcagaagaca tcaaagagat gaaagccaat aataaatggt cgtggggtta
    22501 catgatcggt ttaggcatta caatcattgg ctatttcttg actaaattgt aaaggaggtg
    22561 agaagaaatg gttttaccag ataagtatta tcaagtcatt aaatggacag ttttaacagt
    22621 attgccagct gcatctgtat tagtagcgac gttagggaaa gcgtatggat ggaatggaac
    22681 agatatgaca gtactcacta tcaatgcagt agcaacattt ttaggtgtta tcactggtgt
    22741 gtcggcttat aatttgaaaa aataggagga aacaaatgaa aaagaaaatt actattactg
    22801 cgatgagcct attaacggct ctttttttat tgccaattaa tgggtttgcc tatactatca
    22861 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtaca
    22921 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
    22981 aacgttcatg gactagtgct tacactgctt acattgtggg tgatggcgga attgtttacc
    23041 aagttggaca acctggttat gtacagtacg gtgctggttc gtatgctaat gccaacagtc
    23101 ctgtgcagat tgagttacaa cacacacata ataaagcaac ttttgaaaag aactataaag
    23161 catacgttga attggcgaga gattccgcta agaaatacgg tattccgctt acattggaca
    23221 cgccttataa ccaaccag
//
LOCUS       DAXWLB010000029        22715 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXWLB010000029 DAXWLB010000000
VERSION     DAXWLB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.029"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            69..3347
                     /locus_tag="ACGE4H_000886"
     CDS             69..3347
                     /locus_tag="ACGE4H_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFF8364323.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3888..5795)
                     /locus_tag="ACGE4H_000887"
     CDS             complement(3888..5795)
                     /locus_tag="ACGE4H_000887"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFF8364324.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5925..6800
                     /locus_tag="ACGE4H_000888"
     CDS             5925..6800
                     /locus_tag="ACGE4H_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFF8364325.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6880..7854
                     /locus_tag="ACGE4H_000889"
     CDS             6880..7854
                     /locus_tag="ACGE4H_000889"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8364326.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8034..9179
                     /locus_tag="ACGE4H_000890"
     CDS             8034..9179
                     /locus_tag="ACGE4H_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8364327.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9316..9660
                     /locus_tag="ACGE4H_000891"
     CDS             9316..9660
                     /locus_tag="ACGE4H_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFF8364328.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9931..11217
                     /gene="efmA"
                     /locus_tag="ACGE4H_000892"
     CDS             9931..11217
                     /gene="efmA"
                     /locus_tag="ACGE4H_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFF8364329.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVSYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11355..11954)
                     /locus_tag="ACGE4H_000893"
     CDS             complement(11355..11954)
                     /locus_tag="ACGE4H_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8364330.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12358..13479
                     /gene="mnmA"
                     /locus_tag="ACGE4H_000894"
     CDS             12358..13479
                     /gene="mnmA"
                     /locus_tag="ACGE4H_000894"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFF8364331.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13725..15146
                     /gene="arcD"
                     /locus_tag="ACGE4H_000895"
     CDS             13725..15146
                     /gene="arcD"
                     /locus_tag="ACGE4H_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFF8364332.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15160..16278
                     /locus_tag="ACGE4H_000896"
     CDS             15160..16278
                     /locus_tag="ACGE4H_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFF8364333.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16338..17630)
                     /locus_tag="ACGE4H_000897"
     CDS             complement(16338..17630)
                     /locus_tag="ACGE4H_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8364334.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17780..18340
                     /locus_tag="ACGE4H_000898"
     CDS             17780..18340
                     /locus_tag="ACGE4H_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8364335.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18469..18846
                     /locus_tag="ACGE4H_000899"
     CDS             18469..18846
                     /locus_tag="ACGE4H_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFF8364336.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18779..20851
                     /locus_tag="ACGE4H_000900"
     CDS             18779..20851
                     /locus_tag="ACGE4H_000900"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8364337.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21062..22009)
                     /locus_tag="ACGE4H_000901"
     CDS             complement(21062..22009)
                     /locus_tag="ACGE4H_000901"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8364338.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22255..>22715
                     /locus_tag="ACGE4H_000902"
     CDS             22255..>22715
                     /locus_tag="ACGE4H_000902"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFF8364339.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQ"
BASE COUNT         7357 a         4144 c         4821 g         6393 t
ORIGIN      
        1 tgacagtaaa aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag
       61 gaatacactt gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc
      121 tcagtacccc ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga
      181 acgaagaaaa tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa
      241 ctagtgaaga tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg
      301 aaaagacaaa aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag
      361 aaagcacaag tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt
      421 ccacgaataa cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc
      481 aagcaatccc caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga
      541 cctatacgaa tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc
      601 atttgtttgg gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat
      661 atgtctggga ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc
      721 gacttggatt ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc
      781 cagctcctgg gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg
      841 aaaaagaaac aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca
      901 cgaactcgat tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat
      961 attattacga tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa
     1021 aattatacgc ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga
     1081 cgatcaaggt agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc
     1141 aagaaagacc tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag
     1201 gaaaccaaga tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa
     1261 ataaagcaac aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg
     1321 aatttgatta tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc
     1381 cagataaaaa ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga
     1441 ttcttcatac agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag
     1501 ggtcagatgc aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat
     1561 acgcatatgc tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat
     1621 tgacgattcc aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga
     1681 aaaatccaga aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac
     1741 tgttcagtca ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa
     1801 aaggattaca gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg
     1861 gaaatacagg aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc
     1921 ataacttagt caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg
     1981 atggatttgc ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt
     2041 atcataatgc agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa
     2101 aagtaatcat caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag
     2161 aaggtaacgg taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga
     2221 tcaatagttt gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata
     2281 ttatcgtcaa tagcagtaca agttttaata acggttcata taatgtggct ctttatacct
     2341 cttcagcagg aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc
     2401 ttgagatgag agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt
     2461 tgaactacta ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat
     2521 caaaagattg gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc
     2581 gctatgaaaa tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc
     2641 aaacagaaaa ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag
     2701 aaacctcacc gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa
     2761 aaccaaatga acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg
     2821 cagaaggaaa tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa
     2881 atcaaggaac gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg
     2941 aaacacagtc aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag
     3001 gagaatatac gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg
     3061 ctcatacaga atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc
     3121 ccccgggaaa tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac
     3181 caaatggaaa accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc
     3241 aaggactccc aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt
     3301 tgttaatcgg tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc
     3361 ttttatgatg taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag
     3421 atgtctcact tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct
     3481 gttcttatta cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat
     3541 cagctgaccc tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt
     3601 ttttctctaa tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa
     3661 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     3721 aatttttctt actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc
     3781 agaagtaact tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc
     3841 caaattcctt cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc
     3901 actctttttt ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag
     3961 aattcgtcaa ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg
     4021 atttgcagta aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa
     4081 ttgacaaaga attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag
     4141 cttagaccgc ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg
     4201 tcagcaaaag gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt
     4261 gtaagatctt cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg
     4321 ttattatcaa atgcatggga aatctcatga gcgataacag ctccgattcc accataatta
     4381 gcagagcttg attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg
     4441 atcacattcc gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct
     4501 gaacgatcca ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa
     4561 acaagagtat ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg
     4621 aataatgctg ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg
     4681 acggcttttt cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa
     4741 acggcgatca tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat
     4801 ttttttccgt agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct
     4861 gcttttttct gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg
     4921 acttggcgga attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc
     4981 cagcttttga ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac
     5041 actggatcag ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt
     5101 ttcaagtcca gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt
     5161 ttgctgtaat ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat
     5221 tgcactgctt gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat
     5281 aactgaacag ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat
     5341 gcataatacg tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt
     5401 tgcgcattct tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac
     5461 agccattctg gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg
     5521 attataggtt gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt
     5581 tgataatatg caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc
     5641 agatcgtctg tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg
     5701 cctgtagctg gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct
     5761 tcatagaaat cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc
     5821 gtgtgcccat tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat
     5881 tcgaacaatc aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc
     5941 gatcccaact gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta
     6001 aaaaattagc cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc
     6061 tggtttttga agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt
     6121 ctccagcaaa atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg
     6181 aagagccaat cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg
     6241 aagaaaaaag gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt
     6301 ttttgaggta taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag
     6361 cagttctcct tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc
     6421 gcaagaaaaa tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac
     6481 cactaggact tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga
     6541 agaatggcaa tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa
     6601 aacgtatacg tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat
     6661 atggcaatca ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt
     6721 acaaagtaca cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg
     6781 gaacagtttc acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt
     6841 taaactatcg aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat
     6901 gacacgttga agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa
     6961 gtgttcggaa cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc
     7021 atcaatatcg aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca
     7081 cctgtcaatg attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt
     7141 gcgaaaacga tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg
     7201 aaacctcatg aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca
     7261 actcgagtac tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct
     7321 gtagataatt tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg
     7381 ggagatgaca tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt
     7441 tcagaatatt tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag
     7501 ggtggttatg tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg
     7561 aacacaggag caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa
     7621 gtatatgcct gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat
     7681 gcaccaatca aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca
     7741 gaaaacttaa caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat
     7801 gaaaatacac cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa
     7861 aaaagaggga aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt
     7921 tgtcacagct ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct
     7981 ttggtttgtt cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg
     8041 aagtgaaaac tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg
     8101 aagcgatgac ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg
     8161 gtcgccacgc gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag
     8221 caaaacctca cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct
     8281 tagaaacagc gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac
     8341 atcctgctgt tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc
     8401 tgcctgtaga tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag
     8461 atacgatctt ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca
     8521 aagaaatcgg tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag
     8581 cgtacggaaa ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg
     8641 ctcataaaat caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata
     8701 tccctgcatt gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt
     8761 tagcagggat ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag
     8821 cacgtaacaa caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg
     8881 aaatccctta tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc
     8941 gcttaattgg cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta
     9001 tttctactgg ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa
     9061 tgtatggaga agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa
     9121 ataccgaaga cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac
     9181 tagtgaagtc attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac
     9241 ttaactgaaa aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt
     9301 atcgaggtga aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc
     9361 tatcgtttgc atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca
     9421 aacggaggca attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga
     9481 ttcaaattaa aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca
     9541 gcaaatggat tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat
     9601 aaattttatt ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga
     9661 tctgtggata taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg
     9721 taaggacgac gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt
     9781 taatattttg ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt
     9841 tagggaaaaa ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta
     9901 tacaaaatga aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg
     9961 aattggaaga gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt
    10021 atggtcgtcc aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt
    10081 ttgagttttg ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga
    10141 ccgctagtgg atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg
    10201 attttcgcgc ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta
    10261 gtttttgtgt ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag
    10321 tcgattatgc cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga
    10381 atggtccaat cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc
    10441 gttcctgtca gttaccttat tttattagat gtattggggg cagtttttgg tgtcggctta
    10501 ttgatttttg tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt
    10561 tttaaagatg caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata
    10621 acgatcaatg gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg
    10681 atgacgctag attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac
    10741 gcagtcggaa tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga
    10801 atgaaaccta ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg
    10861 gttccaaacg atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt
    10921 gcaacgccat atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat
    10981 gttttaggtc gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga
    11041 ttattatttg caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc
    11101 ggaatcggga ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat
    11161 gataaagaat tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat
    11221 ggaatggagt tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg
    11281 gcaataagcc tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat
    11341 atagggcagt tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt
    11401 tgttgctcag ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt
    11461 gcgtctacgc ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca
    11521 tgaagattgc ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat
    11581 tgttgatggt cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg
    11641 tcagtaagcc taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc
    11701 cctttgatca agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt
    11761 gctttttcat attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct
    11821 tttattactg cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag
    11881 agccgcggag caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg
    11941 ataaagtaat tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat
    12001 ctgagcaact cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg
    12061 ataagaagca attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga
    12121 aaggctggcc ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg
    12181 attttttggt tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac
    12241 gacttgataa ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa
    12301 agttgcgacc ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg
    12361 acagacaaca gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta
    12421 acagcacttt tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg
    12481 gatgacacgg acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa
    12541 gtagctgctc aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat
    12601 cgtgtatttg aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg
    12661 atgtgcaaca aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc
    12721 gattatgtag ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac
    12781 atgcttcgtg gagtagacaa taataaagat caaacttact tccttagcca attatcacaa
    12841 gaacagcttt caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca
    12901 attgctgaac gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt
    12961 attggtgaaa agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat
    13021 atggtcactc ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt
    13081 ggtcaacgtc aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt
    13141 gtaggaaaag atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca
    13201 ttgtatgcca caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca
    13261 aaagaattca agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt
    13321 cgcttattgg atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact
    13381 cctggacaag cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac
    13441 cacgcatatc aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa
    13501 cagctatggc taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa
    13561 taaaattaat ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc
    13621 aataatatga ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc
    13681 ttttgcaagt gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga
    13741 cgggtattag caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg
    13801 tgtttgggat cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat
    13861 ggataatcgt tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa
    13921 agcgtccaga tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag
    13981 gtggcttcat cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg
    14041 caacgatgct gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg
    14101 ttccttctat tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg
    14161 gagttgaagg agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat
    14221 tcctattttt aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt
    14281 ggggtaacgt atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc
    14341 agattaaagg ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg
    14401 ttttagccaa ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc
    14461 tttgtttatt gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag
    14521 aagagttagc aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa
    14581 attggggagc agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt
    14641 cttggacgat gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga
    14701 aaggttgggg aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa
    14761 tcttgactaa tctattctta ttgactttct tagttactga ttatgcctat caatttgcct
    14821 attctttatg tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc
    14881 agttttcttt aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt
    14941 ttgaattgat gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct
    15001 atcttcctgg tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg
    15061 gaaaagaaaa agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc
    15121 ttgctgtagg ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt
    15181 gtagaagaat ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg
    15241 atcgaagcat tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt
    15301 ttcgaaaaac gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc
    15361 aaacaagaag tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat
    15421 ggagcgactg gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt
    15481 gtttcgatgc ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct
    15541 gttgactttg ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa
    15601 gccttagtca aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc
    15661 acattgctag atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca
    15721 tatgtattga tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc
    15781 gtagatgtct atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca
    15841 ggtattcgta ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat
    15901 cgagactaca cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg
    15961 aagaataaag aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt
    16021 ttaaaacaat gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct
    16081 acttctttca ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta
    16141 gaagaaacag gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga
    16201 ctaggttact gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc
    16261 ttgaaaaaac aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca
    16321 gtgtttttca tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact
    16381 cctgaagctt tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca
    16441 agcaagaagt tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat
    16501 gcactggaaa cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat
    16561 tggttataac tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct
    16621 tggacagcag ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat
    16681 tgccaaacat tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca
    16741 atcgcaagta ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct
    16801 atcaaagtgg caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca
    16861 tgtccatgca gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat
    16921 gcttggatga tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct
    16981 ttgaaaatct gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg
    17041 acgaaaaaca gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta
    17101 gggcgatagc caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct
    17161 gtaacagaac cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca
    17221 gttgcgacaa gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc
    17281 aatgcgacta attgatacac actagtcacg caacccatca aagaaatgac gattgccatt
    17341 cctaatgaag cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac
    17401 caaggagaga taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag
    17461 ttcaattgtg acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg
    17521 actaaactaa atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt
    17581 tcccaagttt ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct
    17641 tctttctgaa aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa
    17701 tgaaagtctc ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt
    17761 tatgagagga aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg
    17821 ctttcgagaa ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa
    17881 acgcttggat atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa
    17941 aacagcgcta acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt
    18001 gggaaaaagt ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt
    18061 ttcaaatcaa gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt
    18121 attagatgga atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt
    18181 aacgaaagaa gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct
    18241 ttccttacgt tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta
    18301 tttttggatg atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa
    18361 caattttaag atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg
    18421 aaaagtgact aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta
    18481 attaccatgt tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt
    18541 gaagaagaag aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg
    18601 aacgaaatcc atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt
    18661 tggaatgacg gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag
    18721 ggacttgcgt tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat
    18781 gaaacaacta attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg
    18841 tgctaatcaa aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct
    18901 tggtcattta ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca
    18961 aatggacacg ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg
    19021 acatcaatta aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat
    19081 tgcgacagat gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca
    19141 tttcaataaa ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac
    19201 tggattcaag caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc
    19261 ccgggcaaaa gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta
    19321 cgcggataat ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca
    19381 agaaaagaaa atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg
    19441 aaaagaacaa gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc
    19501 acaaaaactt gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa
    19561 attaaaaacg gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa
    19621 tcaacgctat ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac
    19681 ccataagatc gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag
    19741 tacgatgaaa gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt
    19801 gaaaaacgga gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca
    19861 ccatgcactt ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg
    19921 aaaaatctat caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt
    19981 cagccaaaca aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg
    20041 tgtcgtccaa gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga
    20101 aaaaggaatg agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa
    20161 accattgtca gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc
    20221 tacgcgtgca gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca
    20281 aggattgcaa gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt
    20341 aacaccagat ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa
    20401 acaagcagga accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat
    20461 caaatcaagc aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg
    20521 tggggccaat ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc
    20581 agattgcaac taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg
    20641 tcaccggaaa atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga
    20701 tggaacgaca gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga
    20761 agaacgacgc ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc
    20821 agcagcacta aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt
    20881 ggtcagcttc aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    20941 ttcggcttat ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt
    21001 gacaagactt ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat
    21061 attattggtg aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa
    21121 caggacgctg tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac
    21181 ataacaattg gattccacca atgaacagga agatcgagac catggaaggc catcctgatg
    21241 taggatcacc aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga
    21301 cacaagacaa agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga
    21361 taccatcaat cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac
    21421 gttcgcggtt ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt
    21481 ttgaaaatcg gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca
    21541 agcggaaatc acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt
    21601 agaatttttt cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg
    21661 tgccgacaca atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg
    21721 gcggatcttg cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc
    21781 cagcgtacag agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag
    21841 gataagctga ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa
    21901 taaacaaata ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag
    21961 gaacagtttt ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc
    22021 tttcaatcgt atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc
    22081 tttaaaattt gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt
    22141 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
    22201 ttttggatag agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa
    22261 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
    22321 tcttttcagc caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa
    22381 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
    22441 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
    22501 gaaatccgaa cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg
    22561 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
    22621 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
    22681 cgccaattgt tgttgttaga aggatatctg caaaa
//
LOCUS       DAXWLB010000030        22690 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXWLB010000030 DAXWLB010000000
VERSION     DAXWLB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.030"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            373..972
                     /locus_tag="ACGE4H_000903"
     CDS             373..972
                     /locus_tag="ACGE4H_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HFF8364340.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            1093..1308
                     /locus_tag="ACGE4H_000904"
     CDS             1093..1308
                     /locus_tag="ACGE4H_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364341.1"
                     /translation="MARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFAKKF
                     IEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            1691..1897
                     /locus_tag="ACGE4H_000905"
     CDS             1691..1897
                     /locus_tag="ACGE4H_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364342.1"
                     /translation="MIENRVLGYVECKDNLDDTLEVLESIIFNTKVLDLKAINGDMTT
                     HIILDEISAKEIGETLIAWANKEL"
     gene            1977..2831
                     /locus_tag="ACGE4H_000906"
     CDS             1977..2831
                     /locus_tag="ACGE4H_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8364343.1"
                     /translation="MMKSKFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            2847..3281
                     /locus_tag="ACGE4H_000907"
     CDS             2847..3281
                     /locus_tag="ACGE4H_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364344.1"
                     /translation="MSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKST
                     GDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKTR
                     PVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            3601..4044
                     /locus_tag="ACGE4H_000908"
     CDS             3601..4044
                     /locus_tag="ACGE4H_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFF8364345.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLFHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSIVFYT"
     gene            4189..4512
                     /locus_tag="ACGE4H_000909"
     CDS             4189..4512
                     /locus_tag="ACGE4H_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364346.1"
                     /translation="MARSRIAKIEQTKKDQKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKDKG
                     IMIPA"
     gene            4930..8559
                     /locus_tag="ACGE4H_000910"
     CDS             4930..8559
                     /locus_tag="ACGE4H_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFF8364347.1"
                     /translation="MKKLKLISVALFSLITLSTARQSVVSAEGTSTKAVVEESGDKKV
                     AIDEKVKNEAEKATVVQGADGLMYVVVDENNPEKLLEVESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFETE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRRALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVIFTIYSSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEITQDTPSTKPAKVKVT
                     NIYEVSVSEKVSDMNESQVLKNVWSADPSDYLQYDITTGNLKKSGERNVQSFTIEGFY
                     DNKNVDVSKSEVYVGSTNVTSSFDISNDTGNGKVVARAKATVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKPLTIVKNSKVTVGSKISKSANSNDVETVLYSRKITINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDLIHKGKIDGEDIVLTTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTELVLSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSIDLPTEYLTKNKKINYSIDILLVENPDKNKFETETKDLRTVGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERANNNPVKEFNENINYKFTDNTKTKTGYGVSVD
                     LEPNYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     NTQNLSFQFPHMNIERKTGNIYADSQKNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKEDELLLEPVYPESTLPDGWNKNEI
                     DWLNK"
     gene            8619..8981
                     /locus_tag="ACGE4H_000911"
     CDS             8619..8981
                     /locus_tag="ACGE4H_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364348.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSMVFTDMVHKMLNGEPINFNFDYMLEKAQSNQVVAFLVSTYYFIEKSIFSLKDF
                     FLGIFTKLTTFYNKILKN"
     gene            8995..9147
                     /locus_tag="ACGE4H_000912"
     CDS             8995..9147
                     /locus_tag="ACGE4H_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364349.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAVLIGTAKVAFPEVFDTVITFFKNM
                     IPTNLG"
     gene            9215..9637
                     /locus_tag="ACGE4H_000913"
     CDS             9215..9637
                     /locus_tag="ACGE4H_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364350.1"
                     /translation="MIYVALFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETTD
                     YIFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            9649..11064
                     /locus_tag="ACGE4H_000914"
     CDS             9649..11064
                     /locus_tag="ACGE4H_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFF8364351.1"
                     /translation="MRVVEEKKISPKIEENPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSANEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            11066..11896
                     /locus_tag="ACGE4H_000915"
     CDS             11066..11896
                     /locus_tag="ACGE4H_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364352.1"
                     /translation="MKVQAMTKRKGLMDVSELNAYQVAYGKPLDYRDYFNYVGLPCLF
                     MGGFSFALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQIL
                     TDDSKTVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVF
                     TQYFEQLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAV
                     FIGAITFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN
                     "
     gene            11896..12693
                     /locus_tag="ACGE4H_000916"
     CDS             11896..12693
                     /locus_tag="ACGE4H_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364353.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKTDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIKPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            12711..13073
                     /locus_tag="ACGE4H_000917"
     CDS             12711..13073
                     /locus_tag="ACGE4H_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364354.1"
                     /translation="MQEQSVMEIIKWFIGLIMIMTMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDGRFTIESDQLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVALSFNGNSVSQIR"
     gene            13087..13233
                     /locus_tag="ACGE4H_000918"
     CDS             13087..13233
                     /locus_tag="ACGE4H_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364355.1"
                     /translation="MQENNVSRIIYLIGGLIILSLAIAGLVEHWPEIKLLIRNFYDNK
                     MIKM"
     gene            13245..13466
                     /locus_tag="ACGE4H_000919"
     CDS             13245..13466
                     /locus_tag="ACGE4H_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364356.1"
                     /translation="MEAIQENRKDTQETIEKNKKTRKKKVDKFTEMTDKIIEKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            13485..13760
                     /locus_tag="ACGE4H_000920"
     CDS             13485..13760
                     /locus_tag="ACGE4H_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFF8364357.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            13869..15695
                     /locus_tag="ACGE4H_000921"
     CDS             13869..15695
                     /locus_tag="ACGE4H_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364358.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVGWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSWGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELAVSPEEPEEVEEIETVSENEEEVEEIFDFEDEEEVENVQKGDLS
                     TSSQNELVAVNDSQVPPYMPSQTPNYSPQYQAPNVLQPVRKHETVQLVTLNEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDVAWKKPYDLDVKKDKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDPKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            15708..16424
                     /locus_tag="ACGE4H_000922"
     CDS             15708..16424
                     /locus_tag="ACGE4H_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364359.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGKPTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     KKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEVKSVDRRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            16437..19295
                     /locus_tag="ACGE4H_000923"
     CDS             16437..19295
                     /locus_tag="ACGE4H_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8364360.1"
                     /translation="MKLVQNNEVKERKNGFVKNSNKILGKLKKVGGFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYRNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQVLDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVLKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            19346..22087
                     /locus_tag="ACGE4H_000924"
     CDS             19346..22087
                     /locus_tag="ACGE4H_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8364361.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLVSAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     RKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDMAND
                     TKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKVENADR
                     IMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELINQKRQE
                     AKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKENLQNS
                     TSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDVRENLQSSSTSQ
                     PKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQNSTSSQGFKSVD
                     VRENVQGSSASQAKTVDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVE
                     DRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLN"
     gene            22111..22446
                     /locus_tag="ACGE4H_000925"
     CDS             22111..22446
                     /locus_tag="ACGE4H_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5592 family protein"
                     /protein_id="HFF8364362.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            22443..>22690
                     /locus_tag="ACGE4H_000926"
     CDS             22443..>22690
                     /locus_tag="ACGE4H_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364363.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRV"
BASE COUNT         8555 a         2854 c         4432 g         6849 t
ORIGIN      
        1 tagtgcttat cttgtctttg ataataaaaa caaagttata aagaaagaat tttaattctt
       61 agtggcggaa aacatttgtt tttccgctta tttttattca aaagtaccca aaatataggt
      121 atactgaatt aaggataaat acaaaactag ttagaggaca gttaaaaaaa tacaaacaaa
      181 ttagattgct attttgttaa attggaagta gaataatacc gttttgatat gtatagtgtg
      241 ttttaccaat aaaattacat aaacgtgaat atattttcat gaattaaatg taaaatgaga
      301 gcaattagtt ttaaaaaaga ttataggctg ttaatcttag catatataat ccattggaaa
      361 gggggagatc caatgaatct aaagaataca aactcaaaca aatctacgct atatgtaatt
      421 tcagatgatt atataaataa gctgaaaaag gtggactatc gtgtccaaaa caactatcga
      481 gaacaacgtt tatattttaa atcaaacttg aaaatttcta acaaaaatga agaaataaat
      541 tattatgtac cattggcttc agcaaaagaa aatcaacgtt ctttgactaa taaatcagtg
      601 tttaagattt atggtgatcg agaagaaaag gactttttag gagtacttca tataaataat
      661 atgattcctg tgccaaaaga ttgtgcaaaa gtatggacac ctaatagccc aaatgttgat
      721 gagaagtata agatgttagt aatcaaacag gcaaaatatt tgaaattaca tgagtctgaa
      781 ataaatgaat catgcgaatt actttataat tctaagttag gtaaacttga tcctagttac
      841 tttaagaaaa atcgtaaaga agtaattact tatcaaaaaa taatgacgaa tgtagaatca
      901 ttggaagctg ttagtatagc agaatcccaa agaaatattc atgtacaaac tcaaagtata
      961 gatgtagagt aagataggct aaacctatct tattttatta ttctattgga tattatgata
     1021 cattgaattt taatcgaata tgtatttaaa tattattata ctgtagaaga taaaacttaa
     1081 aggatgattt ctatggcgag accgagaaaa tataatacag aagaagaaag aaaagaagca
     1141 atgaaaaagg ctaggaaaaa atgggttgaa aaaaatcagg aaactattgc ctatataaga
     1201 gccaaatcat ttgctaaaaa attcattgaa atgtctgaag atgaagatat tgaattgttg
     1261 gaaaaatggt tagaagatcg gaaaaataac ggtcaaaatg atgaataata accattgact
     1321 acaagtagtc aatggtgtag aatggtttta gggatagaaa tatccctttt ctaaaagtct
     1381 aatgactaca agtagtcaat tgagggatcg ggaataaaaa cagacgatta tggcctaaag
     1441 aaaggaggaa tttgaatatt ttgaggaacg atttattaaa tgagcgttga taaatttcac
     1501 taaatatttt cttaatatct atgtattgtt caagcgtaga aaaaaatatt gaaattatta
     1561 caaaaccgct cataatggaa aataacgaat tatattaatg aatcgttcac ttttgacctt
     1621 ttttactaga ttttttttgt aggactttta gagaattttt ttgttatctg attataagga
     1681 ggagctaaag ttgatagaga atagagtact ggggtacgta gagtgtaaag ataatttaga
     1741 cgatacttta gaagtccttg aaagtattat atttaacacc aaagttctgg atttgaaagc
     1801 aattaatggt gatatgacaa cacatatcat attagatgaa attagcgcaa aagagattgg
     1861 cgaaacttta atagcatggg ctaataaaga gctgtaaaag ataattttaa aataaagtgg
     1921 ccaattgact acaagtagtc tgtatagtat aataaattat caaaagtgag gtatacatga
     1981 tgaaatcaaa gtttttggga tatgtaattg caatcaacat gtttaaaggg ggagttggta
     2041 agtcaactct tactcagcta atggctgata ttctatcggc tagaggcttt aaagttttag
     2101 ttattgatgt tgatccacaa ggaacagtaa ctaaaaagtt tcaaagacaa tatgatttaa
     2161 aagttgttgg gaaaatgaca ttgtttgaag cagtaattaa tatgtctttc ttagactgca
     2221 tttctcctgt taaagaaaac ttagatattg tgcttggttc agaacaaatg tcaaggttta
     2281 ccaaagtatt aagagatatg tggagaagta ctggttttac tgatgaaggt gaagaagtct
     2341 tttggaattt gtttaattat atgatcggta atgataattt aagagatcaa tatgatttta
     2401 ttttgtttga cactattcct acagtttcag aatttacaga taacgtttac gtggcgagtg
     2461 attttttagt aattcccacg caaactgagc aagattcgat agacaacttg cacagtacgg
     2521 ttactaatta tcaaactgct gtaaaagaag taaacggaga tttagaaatt ttaggtattg
     2581 ttccatatct agtggatact aaattaaaaa caggattaga acttttatca gaacttcaag
     2641 aagaatatgg agacttagtt tattctcata ttgtttcaga acgaggcgta attaaacgtt
     2701 ggggtagaga aggactaaat aaatctatcc cacattctaa aactgcttat agcatgtatg
     2761 tagacattgt agaagaaacg ttagtacgtt tagaaaaagc tacaggagaa aatattttag
     2821 aacgtgttta ggaaaggtgt tgcatgatgt cgaaaactaa aaaagtaact agtgccggca
     2881 gtaatggttc gggtaatccg ttcggaataa aaagcggtag tggtggattt aatataggac
     2941 aacaaatgtt aaaaggtaaa gctggcatta agtctactgg tgataaacgg aaatctgtta
     3001 gggtaccagt tgagttatac gatgaagttg ttaaagattt atcatacggt gctaaatatt
     3061 cattaatgac agaaatttta gctttatttt taaaagaatt tgaaacacac ggaaatagta
     3121 tagttactga ttttggcaag ttgaaaacga gaccagtaaa aatttctgaa gaagccttta
     3181 aagttttgaa agttataaaa tttgaaacag atcaatctaa tactaatatt attgcttcag
     3241 ctttgacatg gtatcttgat aaaagaaaag gaaaagcata atgaaatgag gtaagactat
     3301 ggatcataaa tcaaattatt catacaaaga aaattatatt tggaagtatg tagtagccgt
     3361 ttttctactg gtaggtggaa ttattgcatt ggttaaatgg gctataattc ctaatgcttt
     3421 agtatttatc aattggtttg tccctaattt ttggagtttt tttggctata tattgataat
     3481 ctggtttatg tttaaagtac tctctttttt ctggtcgcct gtatattctt tgcttggaaa
     3541 gtggtcgtac ctaattttaa tagcgacagc tatttatatc attatgcaga ttaactaatt
     3601 atgcagattt tagaagatat taaaatttat tggaaaagga taataggcta tacattagtt
     3661 cttcttgtag taaatttatt cctttcgatg atagcgttgg tctttgttaa tcaaagaaaa
     3721 gacaatgtaa ctgaattagt aacgtttatt tcgtttttca taagtgggat ttttgctcct
     3781 ttctttgaag aaaccttaat gagagggatt atccaaaacc aattggaatt aaaaacaaat
     3841 ttatctacaa gaagcatata tataatcgtt gcaattttgt ttagtctatt ccacttaaat
     3901 ttatatttta ttccttattt tatgacgagt ttaattttgt cttttgtcta tgataattca
     3961 gataaaaggc tgattgttcc ggttactatt cattgttttt acaatttaat tgtaatactg
     4021 ttaagtatag ttttttatac ttgaaaaaaa tagacgaaaa cctaaaaata caaggttttt
     4081 atctattttt tttgttttgt ttttatctat caggttgata gaaattgtca taaatgatat
     4141 aattaagtat gaactgattt agtaaaaaaa ttaaaggagt gatagctgtt ggcaagatca
     4201 agaattgcta aaattgaaca gactaaaaag gatcaaaaaa tttctaaaaa taaaacaaaa
     4261 ttctttaaag ctgaagatgt taagaggata gcatggtctg tgaaaacatt tgatgaagat
     4321 cgttatgtta aagtttcaat gaatggtctg tttgtacagc ttggtaactt cagtaatttt
     4381 aaaacgattg acgaacttta tccggaaatt agaaatattg tggatagtaa aggtttaatc
     4441 gtggctaaac ggaaatcaag aaactctggt cttgcttact gtaaagataa gggaatcatg
     4501 attccagcct aaatcagtga atttgttcct tgaaaactta atactactcg tactgtagca
     4561 atgttgctat gttacagata aaagcgtagg gctaacaagc aaaatagcaa ccaagcatac
     4621 tgttatttcc tttgccaaga ggtatgtaaa tacgttagcg atagaaatga taaggttagt
     4681 atttcacgcc aaaaaataca aaagtcgatc cattgaaact actaattatg aggtggcgta
     4741 aatggtaatt attcaaagtt tctatctagg aagaacctat gaaaatcgtc ctagagatca
     4801 gaatattgac actaaaaaat tcagcacagt aataaatgtg ctagacacag ggtaactctt
     4861 tactacaaga aatgtagtaa agagttgtta tgattcttta ttacatttct ttctaaagga
     4921 agtgtgataa tgaaaaaact gaaactgatt agcgttgctc tattcagcct aatcacttta
     4981 tcaacggctc gccaatcagt tgtttcggct gaaggaacgt ctacgaaagc agtagtagaa
     5041 gagtctggtg ataaaaaagt tgctatagac gaaaaagtga aaaacgaagc ggagaaagca
     5101 actgttgttc aaggtgcaga cggcttgatg tatgtggtcg ttgatgaaaa taatcctgaa
     5161 aaattgctag aagttgaatc tcctttgaaa aatcaacaag aaaataccgg cgctgttgaa
     5221 gtcggtgttg atgaaaagag acaagaaaag gctgataaag ctaacgaaaa agtgaaaaac
     5281 tttgacgata ttgattacaa cgatattagt tcgttgcagt cagcgttgaa tgatccgaaa
     5341 ttcccttttt tatcggaaga tgaattaaat gaaaaattgc aaaagacaaa gaatacattt
     5401 gagactgaag cgttaatgaa aaagacaagg ggatatttca gtcaaaggat tttagagtta
     5461 agcaataatt taaatgacgt accgaaaaga gcgaaagtga gtcggcgtgc tttagttagt
     5521 aaccgtgctt cacaagtaag tgaaacacga ataaaccctc caaatggtta tccgtgggtg
     5581 atttatttaa ttaggagcaa tggttatagt tatacagaaa attatgctat tagaagaact
     5641 attgatggga aagaagcttt ttgtttacag gccggtgtac aagcgacgaa tacatacact
     5701 ggttcaacaa gtggttatct ttctaattcg caaagagaaa tgattagttc gattttatca
     5761 gcatataaag aattaggtgg ttgggctaat aatgatgccg gaaaacaaag atggatagca
     5821 actcaaatgc ttgtttggga aaacagtaat gaaaagccta aaagtataac tgttacgcca
     5881 aattggaaaa acggagctac taagtggaaa aatgatttag tagctcgtgc aaatgctatt
     5941 agagtaagaa caaaatggga ttctacgtct aagacggttg atgagggaga caaagcaacc
     6001 tttaaagtaa ctaatactta tggagatcat aatttgcatg tgaaatctgt atctggtggc
     6061 ggcaaagcta ccataagcaa tggtacttta actgttgata ctacaggagc cactggaaac
     6121 aaaatcactg tagatatgat taaaggttct cacccttcag acgcgcaccc tacaacggta
     6181 tggagtaatg gttcttatca aaagcttgtt accggttctt tttacattag tacttctgtt
     6241 acggtaaatg ttaagagaaa aggaactgtt gaagttttaa aagttgattc agaaacaaaa
     6301 aaaccattat caggagcaac atttagattt agctactcag gtaaaacaaa agatgttact
     6361 actgattcga atggtaaagc aaaattaact gaaaggttga aagctggaac tgtagtaaaa
     6421 gttaaggaga taaaagctcc taatggctac caactagatt caagcgaatt ttcaattaca
     6481 gttaaagaaa atcaaaatgt tactaccaca agaaccaata agaaatccac aggttctgta
     6541 gaaattgaga aaataggcga tttagatgga ttaggattgc cgaatgtaat attcacgatt
     6601 tatagttccg ataataaagt tgtaaaagac aacttaaaaa cagatagtaa tgggaaaata
     6661 aaagttgatg aactacaatt tggtaaatat tatgcagtag aaaagcaagg tgttactggt
     6721 tatgatcccg atggaaaaaa atacaatttt gaaataaccc aagatacacc ttccacaaaa
     6781 ccggctaaag tgaaagtaac caatatttat gaagtatctg taagcgaaaa agtatcggat
     6841 atgaatgaaa gtcaggtatt aaaaaatgtt tggtctgctg atccaagcga ttatcttcag
     6901 tatgacatta caacgggaaa tctaaaaaaa tccggagaaa gaaacgtcca aagctttact
     6961 atcgaaggtt tttacgataa taaaaatgta gatgtttcaa aaagtgaagt atacgttgga
     7021 tcaacaaatg taacaagctc ttttgatatt agtaacgata ctggcaacgg aaaagtagtc
     7081 gctagggcga aggcaaccgt gttaacgaat aatgattttt ataataagtc ctataattta
     7141 cggatcacta tgaaaataaa aaaatctacg cttgaaaaca ctgaaaagca aaaaccctta
     7201 acgattgtta aaaatagtaa agttactgtc ggttcaaaaa tatctaaatc cgctaactct
     7261 aatgatgtgg agacagtttt atattccaga aaaattacta tcaatcatat cgatgaaaaa
     7321 gataaacacc ttttgaaaca agatattgat tataaatacg atggtgaaac atatgaatat
     7381 aaacctagaa cagatttgtt tgataaagat ggaaataatt ataaatctga tttaattcat
     7441 aaagggaaaa ttgatggaga agatatagtt ttaaccactc cttatcatat tccggtgctt
     7501 tctgtgaatg tggatcggat tcaaattgat acggctagag ctgttaaaaa tggctattta
     7561 cctacagaat tggtgctaag caaaaaagaa gaatatgaaa aagagttaga aaaaattgta
     7621 tttaaagtga aaattacaga tatagataac aataaagttg tttatgatga aaacttcaaa
     7681 tacaaagaat ttaatagtca tatgtcgatt gatttgccta cagagtattt aacaaagaat
     7741 aaaaaaatta attatagtat tgatattctt ttagtggaaa atccagataa aaataagttt
     7801 gaaactgaaa ccaaagattt aagaacggtc ggttatactt cttctgaaaa agttttaacg
     7861 aacaaagata tgacaaataa ccaagtcaac tattcagcag tagttagaac agtgaaagaa
     7921 agagcgaata ataatccggt taaagagttc aatgaaaata tcaattataa atttactgat
     7981 aatactaaaa caaaaacagg atatggtgta tcagttgatt tagaaccaaa ctacagaaat
     8041 gaaattggga agcaaataga tatgaaagtc tatagttatc cgatgaatga attgattgac
     8101 agcaaaattg acgaaatcta tcaaagtggg ggaaaagaaa cagaggttaa gatggatcgc
     8161 ttaaagaatg atgggaaagt tgagtcgggt attaacactc aaaatctttc attccaattt
     8221 ccacatatga atatcgaaag aaaaactgga aatatatatg ctgacagtca aaaaaatgat
     8281 gaatctatta agtatgaatt gatcgatggc ggtagaaaat tatatgtgcc tatttggtgc
     8341 gatttgggga attattctat tgctatcaaa agtagtaact ttggtggtaa ctatatctcg
     8401 ttggatatga accagaacat agatgtttat gcttatatgt atgcaacgat agatagtgaa
     8461 actaaaaaag aagatgaatt attgcttgaa cctgtttatc cagaatccac attgccagat
     8521 ggttggaaca aaaatgagat tgattggttg aataaataag tttgaaaagt ttttatctat
     8581 ctgatagaat taaacaatga tatagggatg gtgatagaat gagggttcaa agctatggta
     8641 acagtgtttt tgatgttaaa agagaatttc aagattcgag aagaaaaacc aaaaagctga
     8701 tagcttactt cattctatca gctatcgttt tctatattgt caagtttgtt ttggaattaa
     8761 actcaatggt attcacggat atggttcaca agatgttaaa tggagaacct attaatttta
     8821 actttgatta tatgcttgaa aaagcgcaaa gtaatcaagt tgtggccttt cttgtgagta
     8881 cttattattt tattgaaaaa tctatttttt ctttaaaaga ctttttcttg gggatattta
     8941 ccaaactaac aacgttttac aataaaattt taaaaaatta aggaggaagt ttgaatgtta
     9001 gatcagaata cagatagaag ttggtggatg attggtgccg taatcgtagg tgccgttttg
     9061 attggaacag ctaaagtagc ttttcctgaa gtatttgata cagtaattac atttttcaaa
     9121 aacatgatcc ctacaaactt aggttaattc cactaaaaaa ttcacgctgt taaatgcgta
     9181 gaaaaataat aaagaagaaa aagggctaca taaagtgatt tatgtagccc tttttttgga
     9241 tggaagtgat gtaatgctaa gagatatgcc caaaatgatt cttctgatgt ttgttgggtt
     9301 ctttttaatt ttagtgattg cgtactattc attaaatggc tatacacaag attcttcagt
     9361 gaaaggtatg caagaaacat taagaactac agcaatagca aacagggatg atagtattcg
     9421 tgtagaaaaa gggcgttttg ctttaattaa atctgatttt gaaaaagatt ttaaagaaaa
     9481 atttgggaaa agtaaaaatc taaataatga aaccactgat tatatatttg attatcttac
     9541 agatggaaag gatggtatta aagcaattaa agtgaaggtt gaaagtggat caaaagaata
     9601 tcaagcaact tgtattttaa atgttgcaaa tgattagagg tgtgaaattt gcgtgttgta
     9661 gaggaaaaga aaatttctcc aaaaatcgag gaaaatccta aaagtaaaat taatatcgca
     9721 agtattatta aaaaaaatgg aattaaagaa aactctgaag cgatagaagg aaatcttgat
     9781 tttttaacta agcttttaga agaagatagt ggaaattatg aaccaccaat tgaagatgca
     9841 aaattgaata tgattcgtga tgatttaacc agaaactatt cccaagaagt tttacaagct
     9901 ttaactgatg atgaaattag gcgaaaactt gtacaagttg ttttgaaaga acatggtact
     9961 ttctttaaaa aagataaaga ggttgcagaa tatgtagtac gtgaatgtat tggaacaggg
    10021 attattgagc gaattatgac caacgatgaa attacagatg ttggttggaa tggctctcaa
    10081 cttagcattg aaactaactc taacaaatgg ttgtttgacg gtgataaatt aaatatcacg
    10141 caagagtata ttgaacgtgt tatttctaaa tatgctgttg taaatgatcg ggcttttaat
    10201 agtggtaatc ctatcatgga tggaatgttt aaaaatgtcc gtatatcagc tacacacata
    10261 gataattcac cagatggctc tacgttttct atgcgtgtgg caaggcctag attagcattg
    10321 aataaagaaa actttgaaga ttttgctcct gattatattt tggagctatt tgagaaaatg
    10381 atgaaaacaa aggcaaatat ctgtatatca ggggagacag gtacaggtaa aacggaactt
    10441 caaaaattac tgctaagttt tattgataat catgatcgta tgattatgat agaggacgtt
    10501 caagaaactc atgccaaaga attgtttcct gataaagata ttttctcttg gatcactact
    10561 cccaaaaaag caatttcaga tttagtaaaa gcttctctta gaaataatcc acgatggatc
    10621 attgtttcag aaacacgtgg ttctgaagct tatgaaatgc ttcaagcggt tttgtcaggt
    10681 cactatgttg taacgacact tcatgcggtt aatgcacgtg cgatacctag acgttttgta
    10741 aatatgtgtg caagtggttt taaaataaac gaaaatgcgg ttgaggaaga cataaatcgt
    10801 tatttcgatt ttggtgttca tatcaaaaaa acagtggttg atggtaaagt gatccgttat
    10861 ttgaatgaaa ttgttgaatt ttctgctaat gaaccgacta cagtttttaa gcaaaaattt
    10921 atcaatggaa agtttgtcat tgaaaatggt agtctttccg aagattttaa agaaaaaatg
    10981 gctgaaaaat ttattacgtt tgaatttcct gaagatcaaa ctaatacaga agaatcactt
    11041 cctgaagaag aagaggtgat ttaagttgaa agtacaggct atgacaaagc gtaaaggtct
    11101 catggatgtt tcggaactaa acgcctatca agtagcgtat ggtaaaccgt tagattatag
    11161 agattatttt aactatgttg gattaccttg tttgtttatg ggtggctttt cttttgcttt
    11221 atgttactac tggtggattg ctttgatttg tggagtaatt ggaggcgtat atggctttaa
    11281 agttataatg cctaaaagta ttaaacgctc ttatgaatta aagagcttac aggaaagaaa
    11341 taaatttatt aacaacatga ctcaaatttt gaccgatgat agtaaaacag ttactagagc
    11401 gttatctata gcaaaacagc gaacaaaagg tgaattaaga gaagatattc aaatcttaga
    11461 atcacgttta caaggtgctg ataaatttca gattgctgaa gcttttcgtg aaatcaacaa
    11521 taaatattat agtgatgttg tttttacaca atactttgag caactggaaa cagctattta
    11581 cgaaggtaga aataattcag atactatgaa gcaaataaaa agttatcaca atgatgtgaa
    11641 acgtaaaaca gaaggtttta tgaaaatcaa agatggctat cttcaagata tgaagcaaat
    11701 gatgtttatt gtagctgttt ttattggagc tataactttt gcttttgggt tttctacttt
    11761 ctatgaaggc tttgctcgat cacctatcgg atgggtattt gggtctattt actttttgat
    11821 tatggttagc ttcatgaagt catttttcaa acattatttt gatgatgaaa taatgagttt
    11881 gggggtaagt aattaatgaa agttaaggct ttgaaaaaga aaagaaaatc aagtattagc
    11941 cccttgaaaa ttacgaaaac agaacataaa agcttttgga ttaaattgct aggtgatgtt
    12001 tattatcaaa aattacgtga aatgaacaat tccaatattg cgattattaa gtggcaaaaa
    12061 caacggttaa aaaagtcatt gatttttatc ctggtcggat tcttaatgta tctattttta
    12121 gggaaagaat ggtattactt agccggagga attgctactt cagtaggttt ttatgtttgg
    12181 caagcaaaat caatcaatgg tatctataat caatttcggt ttgaacgaca cttgcaattt
    12241 agtaaattta cacgtctatt gattccatat ctgaaacaaa aaacagatgg cggaaatcta
    12301 tacggtgttt ttgcaaaaat cgtaaaaaga ctagattatg ataccgataa aaagctatta
    12361 atgaagctta tgcaagaaat gacagatcag cctaacgata ttaaaccttt tacggaatat
    12421 gcagaacaga cttccggaac agatatgtcc gtattattta tgtctacagt ttatgatatt
    12481 cgacaaggat cggcagattt agatgtgatt gaagagttgg atcgattagc ttctgaagaa
    12541 ttgatggccg gaatcgattc aatcattgac tataagtcga ggcgttttgt cttctttcct
    12601 acaaaaattg caatgacttc atttatttta gttgtcggtt ttgcagtagg cgttcttttc
    12661 atgaacctaa aagatattaa ctttgggcaa taaaggaaag gagaaagtca atgcaagaac
    12721 aatccgttat ggaaatcatt aaatggttta taggtttaat tatgattatg acaatggttt
    12781 ctataggact cttttgtgtt caattacaag atattaatac ttttaagcaa caagtaaatt
    12841 accaaattga acgaaaagga ggtctgacta cagaagctgt tgaagcaatc gacaagtatt
    12901 cggctactaa ttatgatggg cgttttacaa ttgaaagcga tcagttaaat cagaaggttt
    12961 cttatggaga agaaattgat tatacgatca aagcaactat tccaattcaa attttaccgc
    13021 ttcctgatgt agctttatcg tttaacggaa atagtgtttc tcaaataaga tgagaggaag
    13081 gataaaatgc aagagaataa tgtttccaga ataatttatt tgattggcgg attgataatt
    13141 ttatccttag ctattgccgg tcttgtagag cattggccag aaataaagtt attaataaga
    13201 aacttttatg acaataaaat gattaaaatg taggaggtat agatttggaa gcaattcaag
    13261 aaaatcgtaa ggatactcaa gaaacaattg aaaagaacaa aaaaacaaga aaaaagaaag
    13321 tagataaatt tacggaaatg accgataaaa tcattgaaaa gaaaggactt aaacctaaag
    13381 actttgaccg acaagcaatc aaagaagcac gacaaaaact gtatgatgat tttgttaaag
    13441 aaaacatatc acagctttta gactaacatt tagggggaaa taaaatgaag aatttagtta
    13501 tcagcaagtt tgcaatgtta ccaagaatgg attttttcga taatgtaagt aagtttatgt
    13561 caaatatgac ttcaaaacta caaggaattt cgctatctgt aattatcttt tgtattgtgg
    13621 ttactggttt gatgtttctt ttcggagaag ggccaagtcg tactgctaaa aaatggttaa
    13681 cgtatatcgt gattggggca ataattatct ttggagcttc aactatcgga caaacgatca
    13741 agtccgttgg tggtttctaa ggggaagctt cttagaacta aaaaattcgc aagtattgca
    13801 tgaaagagca ttgagtgatt aatgctcttt tttattttga aaaactataa actaaggaga
    13861 tttaaactat gaaccaacga ttcgaatata attttgaaga tgtagaacct aagttgttga
    13921 aaaaaggaaa agggttaaaa aagaaaaata aatatactct caaacaagca gaagcactag
    13981 taggatggat tcaacacaat tggtcagaca agacaacaat taccttgatc tgttcagata
    14041 cagaagaagg aacaaagtat gcttttgatg gaattgaaat tactccattt tcgtggggcg
    14101 gacaagaaaa actattgttt cctgatattg aaggtcaaat tttacaagcg cctgacagca
    14161 tgatttccga tcctaataaa ctgattttgg atattaacga tcaatacaaa cacgaaccag
    14221 acgtagcaga accaacaaaa gaatcaaagt caaaattaaa tctgaagctc ccttcagtag
    14281 tattaccatt tgctaagaag aaaaataaac ttatgaatga attgattgaa ccggaagaaa
    14341 cggatattta tgaagaagaa ttagaagaac aagaagagat tgaagaatta gctgtttctc
    14401 ctgaagaacc agaagaagta gaagaaattg aaactgtttc tgaaaatgaa gaagaagtag
    14461 aagaaatttt tgactttgaa gatgaagaag aagtggaaaa tgtacagaaa ggcgatttgt
    14521 ctacaagtag tcaaaatgaa ttagtagctg taaatgattc acaagttccg ccatatatgc
    14581 cttctcaaac tcctaattac tctccacagt atcaagcgcc taatgtctta caaccagtaa
    14641 gaaaacacga aacagtgcag ttagttacgc ttaacgagta ctgtgatttg ggagaagaaa
    14701 ttgaaacttc aatttctcaa atggtcgaaa agttgaaacc agaatcttta ttcaaattca
    14761 tagggattcc ttctacaggt cttacaaata ctcgtattga tgaatataga agtaaccatg
    14821 ctatgttgcg attagctgaa gtaaaatttg aaaatttaag agagcattat tcacgacaaa
    14881 ttatttcatt taaagcagaa gcactagatc gtttaagaac tgcggtagat gtcgcttgga
    14941 agaagcctta cgatttagat gtgaagaaag acaaagccga agagttatca aaaatggaag
    15001 cagaagcaga agctaaatta aatgagttcg tgaaacgtca ggaaaacaat gtaaatgata
    15061 aaatcaaaaa gtttgagatc gaacaagaaa tagatttaaa aaactttatt gcaagacaag
    15121 aggcagacaa agcggtattc attgctacag agaaagatcg gactcaaaat ttaattgatc
    15181 ctaaagcgga atcaatcaat gcagatttac ttgaaacaaa agataaattt attgatgaag
    15241 aaatgtacaa aatgaaaatt gaaatgaaca aaaatttatt tgatggcaag caaaaaatta
    15301 aatcagaatt ggctactgaa ctcgctaagg caaatgataa tgtttgggag aatgcgctga
    15361 aatttattca agaaattcaa gatgaaattg aagctaaaac accaatttgg gctgctgaaa
    15421 tcaaggagtt taatagttta gaagaacaag aacatgaacg tatgaagcaa actaaagaac
    15481 ttcaattaaa agaggaaagt attgaaatca agcgtttgga agctgaaatg aatttgaagc
    15541 gtatggaaga attagatcat gaaaataaaa tgcttaaaat taaacttgaa actgcccttt
    15601 caaaaaatga gttactagct atggaacaag atacaaagca agtggtgaat ggatcaaaca
    15661 gagttggcgc acttaattta tttagtaaac gttaaaggag aacggatatg agtgttttta
    15721 ataataaagt tatgttaggg aactgttatg tgaaccacat tggtttttac gatggaggaa
    15781 catacagagt tttattctta cgtttatctg tttttagtta ctcaaaagat ggaaaggggc
    15841 attatgaaaa tattaatgca caaatttctt gtcctttaga tagaaatgga aaacctacga
    15901 ctacaagtag tcaccctaaa gccttgatga ataaatttat ctttgaaaag ttggatcgca
    15961 atttggttga ggggatgaag ataagtattc atggaaatat tcgtggaaca gaacaagtgc
    16021 ttcttaacag tggttggaaa aactttggtc aattatcgcc tttagaaaaa gaagaatatc
    16081 aaaaaattcc taaaaatgaa ttagtaacaa gaaatcaaac atatatctat atagaagagg
    16141 taagtatgcc agaaaaagaa gaatttagaa agtctcaatc taaggtaaaa aaagaagatg
    16201 tggaagttgc agaaccgaat caagcagatg aaattttatc cgatgatact gatccagtga
    16261 atgatgaagg agaggttatt gaagtttttg aagctgaaat tatcgaagag gaagaagaag
    16321 ttaaaagtgt tgatagaaga atgtatcgag agaaaccaaa aggttatgtg aattttgaag
    16381 atatgtatta tgacgaatat tctaatgaac caccgatcaa ttagtggggg tcaaatatga
    16441 agctagtaca aaacaatgaa gtgaaagaaa gaaaaaacgg ctttgttaaa aactcgaaca
    16501 aaattttggg aaagctgaaa aaagtaggtg gtttcaaaaa aaataaatat catactggaa
    16561 aaattgaggg agaagattta ccgaaaagtt tacaagataa attgaatgtt ccgcttgtat
    16621 ttataggtgt ttttttaggt tcaatagttt ttctagcaat aacatactta ttgaactttt
    16681 taaaagcttt gttagttgca atcggccagt cagaaagtgg gctacttggt gtagagttcg
    16741 atcctaaatt tagtttggca tatctaaaac cacttgggga tacgaaacaa tactttatgt
    16801 gtgctgtggt tgcggtaata ggagtcgctt atttaatata tgggaaatta aatcatgaaa
    16861 gtgagaaaaa tcttgtttac ggtcaaaaag gagattcaag actttcaact atcaaagaaa
    16921 ttaagagaga ttaccatgag ataccagaaa aaacagaagt gtatgatggt attggtggtg
    16981 tgccaattag tcactatcaa gataaatatt atattgatag agacacagta aataccgttg
    17041 ttttgggagc ttcacgatcc ggtaaagggg aaacatttgt tgttccttta ttagataatc
    17101 ttagccgagc aaaaatacaa agtaatatgg tcgtgaatga tccgaaagga gaattgttta
    17161 gcgcttcaaa ggaaacactt gaaaaacgag gttatcgtgt attagtgcta aatattgatg
    17221 atccacttga atcaatgagt tttaatcctt tacaactagt aattgatagt tgggctaaca
    17281 aagactatca cgaagcgagt aagcgagcga atactttaac ttcaatgctc tttgcttcag
    17341 gaatgggtac agataatgag tttttctata aatcagctaa atcagcggtc aatgcgatta
    17401 ttttaacgat tgtagaacac tgttttaaca atgattgcat tgaaaaaatt acaatgtaca
    17461 acgtggctca aatgctgaac gaattaggaa gcttatttta cactgatcca aagactggaa
    17521 aagaaaaaaa tgctttagat gaatatttca atactttacc acaagggaat caggccaaga
    17581 ttcagtatgg ttctacttca tttgccggtg ataaagccaa aggttcgatt ttatctactg
    17641 cttcacaagg tattgaaatg tttacaagtg atttgttcgg gaagttaaca agtaaaatgt
    17701 ctattgattt gaaagagata ggctttccta agtccataca atttaaagtc aatacgaatt
    17761 tagttggaaa gaggattagt atttcatttt tgagaaaaga aaatgatgta attagattaa
    17821 ttaagaagta tcgagtgaaa gtaaaagctt tagggctatg tgttttaaat tttaatgaat
    17881 atcttcagga tggagatatg ttggatatac gatatgaaga agagaaatcg agagcatggt
    17941 atcgtattga atttcccaaa aaacaagcaa atagtcatga tacttcttta gtcactaaaa
    18001 aattcaagtc aggtaaaaat tattctgact tagaaatcag caccttacga ttaaaataca
    18061 cggatcagcc aactgctgtg ttcatggtta ttccagatta tgatccgtca aatcacgtac
    18121 ttgcaagtat ttttatcagt caactttata cagaattagc aagtaattgt aagcaaacac
    18181 cggataaaaa atgttttagg cgagttcatt tcttgttaga tgaatttggg aatatgccgg
    18241 ctatagacaa tatggatggg attatgacgg tatgtctagg aagaaatatg ctttttgact
    18301 tagtgattca atcctatagt cagctagaaa caagatatga taaagctttt aaaacaatta
    18361 aagaaaattg tcagaatcat attttaatca tgtcgaacga tgaggaaacg atagaagaga
    18421 tttcaaaaaa atgtggtcat aagacaacaa taaatagaag tgctagttct aaacatttag
    18481 atactgatag ctcggtcact agttcggcag agcaagagcg agtaattaca gttgagcgtg
    18541 ctagtcaatt gattgaaggg gaacaaatta ttttaagaaa ccttcataga caagatacaa
    18601 aaagaaataa gattagacca tttcctattt ttaatactaa agaaacaaat atgccgtata
    18661 gataccaatt tttagcagaa gactttgata ctcaaagaga tattaatgag attgatattg
    18721 catgtgaaca tatcaatcta tctttacaag ataaccaaat tccatatgca aggtttatca
    18781 aagatttaaa aacacgtttg gagtattcaa ttattaataa tattcctatt agtgaagaag
    18841 actatcagga ttatcgaaat ttatcaggat cacaacaaac aattaatgaa gaagagctgt
    18901 taaaactggt aaatacggaa gtaaaaagtc aagatatgga aggtcaagtt ttatcagaac
    18961 aagaaatgaa aattaatatt tatctaaagg aaagtcaaaa ggaagcaata gaattagtaa
    19021 aagtaattaa tggaaggtta gctgatgaaa attcagcaga tatggttata aaaaccatta
    19081 attcacaagt ccttgatacg cctttcattc aatctttaat caatgattct atagctattc
    19141 ataatgttca agagagtaag gatcaaatta tgatcctaga acaacaatta gctaatgctt
    19201 atgagttagc agtgttaaaa acgaaaaatt caatactgaa aaataaaatt atcaagttga
    19261 aaaatgctat gactaagcta gcaaaagcaa tttagaaaga agggaagaaa tattgactga
    19321 atctattaat tggagaaaac taaatgtgca atggttaaag tataaatggc caatcatagt
    19381 ttccctaata atcattctat ttatggccta tccgcaacga gtttttgcgt tgccgtggat
    19441 tacagacaat aaagaggtat taaaattttt aacaaaatat caagattatc ttcagtttac
    19501 taaaggtggt tttggaagca ttgtcgttca aagtttatcg tgggggctga ttagaggtgc
    19561 atattttgtt tgtaaatcag cagaaaacct tgttccacaa tcgctaagtt tgtttagctt
    19621 tgttgaaagc agtagtttag gtgctgttta tcaatctgta ttaaacacgg tggttgtagg
    19681 attaatgatc ctttcattga tgtttgttgg ttacaaaatg attactggaa aaggaacagt
    19741 ggatttaaaa tcggttggaa tgaacgtagt gatgtctata gcattaatac ttttaatgcc
    19801 aaccatgatt tctagtggta ttcaatttgc taaaacattt tataaggact ctactacgat
    19861 tacaaaagct gatgggggcg ttgcttggtc attgattgag gacggtgtaa ctgatttagt
    19921 atatatcaat aaaaacgatg cttatgatcg gttgggtaaa gatggcgtac aaaaaaatgc
    19981 gttaaaagaa aagaatttct tttcctacga tttcagtgaa gtaatcacag ttgatacagc
    20041 taaagattta gaagatgata ataaaaaagc tgaatatctt caatctaggt tgacaccaga
    20101 tggcgaagga aatatgcaag ccgttaagat tgatgatggt tggatgtcga tgttctctga
    20161 tagtttcaaa ggagggtact acaggtttac taaaaatact tttgcattga ctttaggatt
    20221 actttcgtta gctgtagcct atgtttttag tatttttatc atagtcaccg ctattttaga
    20281 actggcattt aagcgtgtgt taggggttct tgtttttgca acagatattg agacaggaca
    20341 gcgttcaaaa gttgttctat cagatattct acagtgctat ttgactgtag gctttcaagg
    20401 atttggatta tccatgtttg caatgtttgt ttcctattta ggaacaggcg gagggtcttc
    20461 cgttaatccg tttattaagg ctgtagctta tgtatgtgcc gtttttgttt taatcaaagg
    20521 cagtggaaca gtcatgagat actttggtgt tgatattgga ctcaaagaag gctatgggca
    20581 acttgtttct gcttttggaa tgggtgcaat gctgtttaga aaaggatcaa atggatttaa
    20641 tcgtgccaaa ggaagcggaa acggaaatgg atcagacagc ggagaaggag aagatcgaaa
    20701 accagaaaag aatttcggtg aaactctatc aaaaaaagct ggcaaaactg gaagagcttt
    20761 gggatatgcc catgaacgtg gattatcagg tttagcttca gatggagcta caatggctag
    20821 tgaaagagca acaaaaccat ttaagagtat gcgtgatatg gctaatgata ctaagaataa
    20881 gttcaaagaa ggtttagatg atggtacagt gtccgctatt aataaaaata gcaaacctat
    20941 gttagctaaa aataaagaag atgaaactgg aaaatatgct gattccatgc caacacgtct
    21001 ttcggataga aaagatggtg ctaaggttga aaatgccgat agaattatgt ctagttctga
    21061 acgtatgcgt gaagcaatga aaaataatgc tgaatcgaat aataatcctg taagtgcaat
    21121 tcagcaaaaa gttcaacaag atattgaaga acgtaaaaat gctatgcacg gtcaagctaa
    21181 gtctgctgaa gaattaatta atcagaagcg acaagaagct aaatacacgc ctgaagcaat
    21241 gaatcgtgag gaaatgttaa ggaaacgtgt agaagggcgt acaggaacta atacggacga
    21301 aaaagaagcg ttgaccaaag aaagaatcca agaagctaaa aactcaaata ccggattaga
    21361 aaaattggtc aaagaaaatc tacaaaattc tacaagtagt caaggtggtc gatcagtaga
    21421 tgtccgagaa aatattcaag gttcattgaa tggtcatgtc ggacggaatg ttgatgtaag
    21481 agagaattta caaagttctt caaatggtca aggtactaag acaattgatg ttcgagaaaa
    21541 tctgcaaagt agcagtacaa gtcaaccaaa aacagtagat gttcgagaaa atgtacaaaa
    21601 ttctacaagt agtcaagggt ttaaatcagt ggatgttcga gagaatgtgc aaggtagcag
    21661 tgcaagtcaa ccaaaaacag tagatgtccg agaaaatgta caaaattcta caagtagtca
    21721 agggtttaaa tcagtggatg ttcgagagaa tgtgcaaggt agcagtgcaa gtcaagcaaa
    21781 aacagtagat gtccgagaaa atgtacaaag ttctacaagt agtcaaggat tcaaaacagt
    21841 agatgttcgg gagaacgttc aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat
    21901 caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat
    21961 tattgatagt gaaatccgtg aaaatgataa gggatcaaaa ccaagacgaa gattcactta
    22021 tgaagacaac gaattattca gagatacact taatgatcct aatccacttt tcgataattt
    22081 aaattaaatt aaaagccggc atgagtgatt atgccggctt tttatgaggt gaaaatgatg
    22141 tatagaaatc ccaaaaatat aaaacaagag ataaagctat ttttcttcta tttagtcgat
    22201 ttaggaatag tagccggaag cgtgatggca agtgtttact ttgcaaaatt aatgccaatt
    22261 tcaccaatga tgaaatttgc actgtactta ttagcatttg tagtgggtgt gtggatgtgc
    22321 gttagaacgc ctaatcaccc taaagagcga aatattttaa tgctgtatta tctctttaaa
    22381 atggatagaa acagatacca cgaagtagag tttaaaagaa atagtagggg gaaaattgaa
    22441 ttatgattgt tgctgaaagt aatgaaaata aattaagtgg tgtttttgac aaattcttag
    22501 aactagttat tccaaaagat aaagatatgt gtgaactttt accatataaa aaaatattga
    22561 aagacggaac gttagtgact aatgacaata agtatcaagc cttttttaaa gttaaaacaa
    22621 ctgacttaac ttctatttca gaagatggat ttatgcgtat ggtaaacaaa ctcacttatt
    22681 taaatcgagt
//
LOCUS       DAXWLB010000031        21859 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXWLB010000031 DAXWLB010000000
VERSION     DAXWLB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.031"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(265..378)
                     /locus_tag="ACGE4H_000927"
                     /pseudo
     CDS             complement(265..378)
                     /locus_tag="ACGE4H_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(761..1993)
                     /locus_tag="ACGE4H_000928"
     CDS             complement(761..1993)
                     /locus_tag="ACGE4H_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFF8364364.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2247..2816
                     /locus_tag="ACGE4H_000929"
     CDS             2247..2816
                     /locus_tag="ACGE4H_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8364365.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2994..3434)
                     /locus_tag="ACGE4H_000930"
     CDS             complement(2994..3434)
                     /locus_tag="ACGE4H_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFF8364366.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3592..4356
                     /gene="map"
                     /locus_tag="ACGE4H_000931"
     CDS             3592..4356
                     /gene="map"
                     /locus_tag="ACGE4H_000931"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFF8364367.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4388..5311
                     /locus_tag="ACGE4H_000932"
     CDS             4388..5311
                     /locus_tag="ACGE4H_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFF8364368.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIVPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5387..6526
                     /locus_tag="ACGE4H_000933"
     CDS             5387..6526
                     /locus_tag="ACGE4H_000933"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFF8364369.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6519..7319
                     /locus_tag="ACGE4H_000934"
     CDS             6519..7319
                     /locus_tag="ACGE4H_000934"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8364370.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7319..8146
                     /locus_tag="ACGE4H_000935"
     CDS             7319..8146
                     /locus_tag="ACGE4H_000935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8364371.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8151..8858
                     /locus_tag="ACGE4H_000936"
     CDS             8151..8858
                     /locus_tag="ACGE4H_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8364372.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8958..9824
                     /gene="rfbA"
                     /locus_tag="ACGE4H_000937"
     CDS             8958..9824
                     /gene="rfbA"
                     /locus_tag="ACGE4H_000937"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFF8364373.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9838..10410
                     /gene="rfbC"
                     /locus_tag="ACGE4H_000938"
     CDS             9838..10410
                     /gene="rfbC"
                     /locus_tag="ACGE4H_000938"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFF8364374.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10432..11460
                     /gene="rfbB"
                     /locus_tag="ACGE4H_000939"
     CDS             10432..11460
                     /gene="rfbB"
                     /locus_tag="ACGE4H_000939"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFF8364375.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11558..12409
                     /gene="rfbD"
                     /locus_tag="ACGE4H_000940"
     CDS             11558..12409
                     /gene="rfbD"
                     /locus_tag="ACGE4H_000940"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFF8364376.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12444..14477
                     /locus_tag="ACGE4H_000941"
     CDS             12444..14477
                     /locus_tag="ACGE4H_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364377.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKQPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14521..15801
                     /locus_tag="ACGE4H_000942"
     CDS             14521..15801
                     /locus_tag="ACGE4H_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFF8364378.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16011..16817
                     /locus_tag="ACGE4H_000943"
     CDS             16011..16817
                     /locus_tag="ACGE4H_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364379.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16829..18049
                     /locus_tag="ACGE4H_000944"
     CDS             16829..18049
                     /locus_tag="ACGE4H_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364380.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18039..19625
                     /locus_tag="ACGE4H_000945"
     CDS             18039..19625
                     /locus_tag="ACGE4H_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8364381.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19664..21802
                     /locus_tag="ACGE4H_000946"
     CDS             19664..21802
                     /locus_tag="ACGE4H_000946"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8364382.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6897 a         3703 c         4542 g         6717 t
ORIGIN      
        1 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
       61 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      121 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      181 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      241 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      301 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      361 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
      421 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
      481 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
      541 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      601 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      661 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc
      721 tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc
      781 ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc
      841 ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat
      901 cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat
      961 tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg
     1021 aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca
     1081 gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa
     1141 aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa
     1201 tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg
     1261 ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag
     1321 gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc
     1381 gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag
     1441 ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt
     1501 tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga
     1561 cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat
     1621 tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg
     1681 cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
     1741 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
     1801 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
     1861 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
     1921 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
     1981 aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat
     2041 accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta
     2101 aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa
     2161 actaattttg catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta
     2221 taaaaaaatt aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga
     2281 ttttggcagc tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa
     2341 acatcgcgaa caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg
     2401 atgacttgag acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca
     2461 ctgttgagca tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa
     2521 gaagagagaa gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga
     2581 tgcagaattt ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga
     2641 atctttcaga agagcagatc gaatctttgt ataatgggac atggatcata gctactggga
     2701 tcgcagcttt gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga
     2761 tccaagacag tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat
     2821 actcaaaagt tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg
     2881 tttttcttac agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat
     2941 aaaagagcca tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt
     3001 tttgcgacta gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc
     3061 agatccactt taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg
     3121 aaatcatcca ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg
     3181 taaatttttc ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca
     3241 ggcagatcgc catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat
     3301 tcatctgcat ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat
     3361 gcttctgcaa caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt
     3421 tttgctaaag ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc
     3481 aaaacatcat tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa
     3541 tgtatgccgc tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact
     3601 ttaaaatcag cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta
     3661 cataaaaatc tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt
     3721 gtcagaaact atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat
     3781 gaatatgcga cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag
     3841 cctttgaaaa acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt
     3901 tctgattctt gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg
     3961 gaagtaacga agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt
     4021 ggtgacatcg gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt
     4081 gattttattg gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac
     4141 ggagaagctg gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg
     4201 gttaataccg gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga
     4261 gacggcggac tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga
     4321 attttgacat cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga
     4381 gaggtacatg aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga
     4441 gacgactcaa aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta
     4501 ttatttactc ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct
     4561 ccacatcgat caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta
     4621 ccagtttatc gttcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc
     4681 cgtttctgca ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc
     4741 gatgaataaa gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc
     4801 actaattgtc attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt
     4861 gggacagctg attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga
     4921 tcagttccaa actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt
     4981 gatttattta attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat
     5041 cttcgctact gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt
     5101 tgcaacgagg atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct
     5161 taattttgct gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata
     5221 cgtgacgggt catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt
     5281 gaaaactaaa ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat
     5341 cagtaattta taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt
     5401 tagtatggtt ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt
     5461 caaaatcatt tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa
     5521 agttccaatg ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt
     5581 attcttattc cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt
     5641 agtaatcata gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag
     5701 tatagggatc ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc
     5761 cttcacgctt ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat
     5821 tttttggatc ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc
     5881 ggcagggatt tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca
     5941 tgcttctaca gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt
     6001 ttttccttac aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct
     6061 agggtttatg atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc
     6121 gatttctttg ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag
     6181 gagaaaagca aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact
     6241 aatcagttta ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat
     6301 gttttctttc acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat
     6361 tattgccatg ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa
     6421 acatcagttt attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt
     6481 aaaacagaaa tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc
     6541 cgatccttat tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt
     6601 ttatttcaac tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac
     6661 ttcccgagta caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt
     6721 ttcatgatga aaattccgga tttggttatg gacataatca caatctgata gctagcgatt
     6781 ctcgctatgg cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat
     6841 tggtcttgtt attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg
     6901 aagatggaac aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc
     6961 gctttgtgcc tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat
     7021 gccgaggatt gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg
     7081 tagctgatat tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact
     7141 ttgaagacaa cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac
     7201 ccttttatgc agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc
     7261 gcgtgttttt gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat
     7321 gaaaccagaa atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc
     7381 ttatggacca ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa
     7441 tagtcctcaa aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga
     7501 tccgcaaaat cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg
     7561 aacacgttgt ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact
     7621 attgcgtaaa gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg
     7681 gagccgacaa gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt
     7741 agaagttgaa ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc
     7801 tgtcaaattt ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga
     7861 tcattggctg ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg
     7921 aatggaacat gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc
     7981 tattttacaa gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca
     8041 ccgacgacaa ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat
     8101 ctggcggatg actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg
     8161 tttgtatagc tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat
     8221 ctcagctttc agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat
     8281 gggatatatt aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc
     8341 gtcaaggatt gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct
     8401 ttttggcaga ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt
     8461 ttgagcagaa tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc
     8521 tgcaagagat ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata
     8581 atgtgataag aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg
     8641 atattttgcc gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag
     8701 gaaagaagac tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg
     8761 cgagtgagat tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga
     8821 ttacggcgtt gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa
     8881 tatttccatg acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa
     8941 aaggagagca gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt
     9001 accctttaac aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt
     9061 attatccaat gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc
     9121 cacaagatac accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga
     9181 tcgaatatgc agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag
     9241 aatttattgg agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg
     9301 gtctgtctaa aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct
     9361 atcatgtcaa tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt
     9421 tgtcgattga agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt
     9481 tttatgacaa tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac
     9541 tagagattac ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta
     9601 tgggacgcgg atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta
     9661 cctttatcga aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg
     9721 cttaccgtat gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa
     9781 aaaatcaata cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg
     9841 aaagtaacag atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat
     9901 catcgcggtt tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa
     9961 tatgactttg ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt
    10021 catttccaaa aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt
    10081 ttagatgtag tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt
    10141 atcttatctg aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc
    10201 gttactttga caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa
    10261 gcagatgggg gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca
    10321 tcaagagcga ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca
    10381 gaaaatccat ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac
    10441 atcattgtta ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac
    10501 aatcatccag aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag
    10561 aacttggccg gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca
    10621 gaattagtgg accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc
    10681 cataacgata attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg
    10741 tatacacttt tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac
    10801 gaggtttacg gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga
    10861 gaaaaattca ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct
    10921 ggttcagatc tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca
    10981 aactgttcaa acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta
    11041 acgagtatct taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac
    11101 tggatccata cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt
    11161 ggcgaaacct atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg
    11221 atcttagaaa tgatgggcca accaaaagac gcatacgacc atgtaaacga ccgcgcaggg
    11281 cacgatttgc gttatgcaat cgattcgaca aaattgcgag aagagctagg ttggcagcct
    11341 gagtttacga acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa
    11401 gactggtgga aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa
    11461 tacagaggaa atttcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc
    11521 tcttttttaa aataaaaatc aaggagcgga taaccatatg tttttattaa ctggaggaaa
    11581 tggacaatta ggtacagaat tgcgccattt attagatgaa aaaaatctgc catatgtttc
    11641 aacagatgca aaagacttgg atatcacaga tgctgagaaa acaatggctt atattacaga
    11701 attaaaaccc gatgttattt tccattgcgc agcttacaca gcagtagata aagcagaaga
    11761 agaagcaaaa gaattagatg agaaaatcaa tgtcgatggc acaagaaatg tagcacttgc
    11821 agcaaaagct gtgaatgcac aattcgtcta tatcagtaca gactatgttt tcgatggaaa
    11881 aaacaaaaat gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg
    11941 tacgaaatta ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg
    12001 aacttcttgg gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc
    12061 ggaaacccgc gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg
    12121 tactttggca gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca
    12181 tttgtcgaac gaaaatagct gttcttggta tgagttcgca aaagaaatat tgaaagatac
    12241 agatgtagaa gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca
    12301 gtattcagtc atgagcctga aaaagacaga agcgttaggt tttgtcatac caacttggca
    12361 agaagcactt gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttta
    12421 tcaaatagaa tttggagcat gatatggaaa aacaaacaag aagtgcacga cgaaaagaaa
    12481 agccaaaagc gaatcaggca gactcgaatt tggtgcttga cctgtttcta aaactccgct
    12541 atcttattgg cgttttagtc atcgtggtca tcgttacttt taatttaaac ggaagttcac
    12601 ttggtgtttg ggataaatat gtgtcgcaaa gagacgatgg gaaaaaaact gatgtgattt
    12661 ttggacagaa tcgggaagtg cggtcagacg aatggctggt gcagacacct ttttacctct
    12721 cacaagctga agaggacttt tcgttggtga acgaggatta ttcattaaac ggacaaaaca
    12781 tgatcattgc gtataattct cctgtaaagg atattacggt tatcggaaaa ccattcaact
    12841 ggggattctt tttcctcgga agagatcgag gactttcctt ttattgggcg atgaaattag
    12901 tcgtcatggt cttgcttgga ttcgaagtac tgatgatatt aacgaagagg aaaaaagctc
    12961 tatcgctaat cggtgcattt gcgctgacat tttctccagc agtccagtgg tggttcatgc
    13021 aacatgtggg cgatttgatt ttctttactt tgggactgat gattgctttt tatcattatt
    13081 tctacaatca tgataaaaaa tggctacgaa cgttgatgat gcttttggtg gttattttcg
    13141 gtattgggtt tattttagtg atttacccag cccatcaagt gatgctggcg tacttgctga
    13201 ttttttattt tgttggcctg cttatttact atagcaagaa agtttcatgg gatctgttgg
    13261 atgtagtgtt gattggaggg gcagttttat ttattggcgg agtcatggtt catttctggc
    13321 taacgtcaaa agacgcattg ttagcttctt tgaatacctt atatccagga aaacgggtaa
    13381 gtactggagg aaagtggacg attggcgaat ttttttcttt ccttacaaat tggaaaattc
    13441 cttttgaaga tatcaactat tcgaataact ctgaagtggc cctgttctat cacttttttc
    13501 caactgtatt cctagcaagt ccatttgtgt tatttggcaa aaaagatagc gaacaaaagt
    13561 tactgggacg tattttgatg attttttgtc tgttttctat cttttggatt accgttgggt
    13621 tgccaaaatg gctagcagaa atcactctgc tgtcttatgt tcctccatta agagccatcc
    13681 ttacttttag ttttgcggct tgtttattat cgatctggtt tatcgcttat atttggagag
    13741 aagatgttat tcctatttgg tataaattca ttttgttggc agtaaatgca tggtcttatt
    13801 tctatgcgat tacgcattcg aaaatggata attatttttc ggattttgaa accatcgcag
    13861 ttgctgtgtt gggaacagtg attttagctt ttgcattatt tggctggaga cgcttgtttt
    13921 acctcgcttt tgtcgcctta gttatcgtat ctggcttttt cgtcaatcca gtcgtagaag
    13981 gtacaggagc gatttttgaa aaaacactgg caaaagaaat ccaaaaaatc gatcaacaag
    14041 atcctggtaa agtttggttg tctgaagatg aattatataa cttcacccca acattgggag
    14101 tgaaatcctt caatacagta cgcttttatc cagatatagc tgcatggaag aaaatcgatc
    14161 cgaaagaaga atacgagaag tattataatc ggtatgctca tacaagagct tttatcgcta
    14221 aggaagatac aaagttcatt ttagatcgtc cagatatgtt ttcggtcacg ttgaattttg
    14281 aagacgcaga aaaattaggg atcgactatg tgatcagtaa acaaccatta actgattaca
    14341 accaattata tgaagcgcag ttcacgcagc tatatggacc ggataaagac ggttatatga
    14401 tttttaaagt agaatatccg gattatccag atgtaccaca aacacaaact caggaaaatc
    14461 agctagtcgt acagtaatag gctagctgag aataatcctg aaatattagt agaaaggaga
    14521 gtgaagataa ttcaagcaat cgtacaaaaa tttgtcaaca aaatgagtgt cggtttcttg
    14581 cttttgttgc tgacgctagt cttttttgtt tggacagcag gggtaggttt tccagcaact
    14641 ttgtttatat ataacaatag ttcattactt atcgctttta gtgtggctat tttgctcatt
    14701 ttaaatgttt ataaagcaaa aggcgtcgat tttctttatc taggtgttgc gcttgccctt
    14761 ttcattttct tttcttactt tcgggaatta agaaatgagt ctttatttgg agacttactg
    14821 tttcctttga ttattttatt ttgtttagta attaaatgga tcgagtggac aaaatgggat
    14881 cgcggtattt atctgattgt ctttacggtc ttcctcggaa tcacgttata tcgtgtattt
    14941 acagagatcc aagtaccaga aggacaaagc atctgggctc caggcaattc gctttctgat
    15001 atttggatca atacgaatac aatcggaagt tctttgatga ctcttgcttt attgatcagt
    15061 ggatttgcca gttcttttga acgatggtat gttcgaatca tcggtgggat agcagtcgtt
    15121 gcagcctttt taggtatttg ggtctgccaa tctagaggtg cattagtcgc gttgattgtt
    15181 tttgcgatat tggatctttt acctaaatcg ttgatgcgtg tcattcgtgc gccatttatt
    15241 gcctacacgg ttacgattct tttagctttg ccgatttctt atctggcggc tgtctctgaa
    15301 aaagtcaatt tgtttaccgg tcgagaagat atttggcata aattctatca gacgctagga
    15361 gaaaaatctg aacaagtttt actaggaatg aaaacattta tcttccagcg aggaaaccaa
    15421 tttttaggaa accacaatag ttataattct attttgaata tctatggatt gataggattt
    15481 gggattgctg ctttgctgct tatccttttt attggtcgct taacgttgaa agcagattta
    15541 tccaacgggc agatgacttt tatctgggcc ttttttgcgg tgatgatgca gtcatttatg
    15601 gaggatacat tgacttcata cccatgggtt ccgattattt atatcctgtt ggctatggcc
    15661 gttcatcgat atgatgaacc agaagttacg gaggaattct atgttatgga agaggcatat
    15721 gacgatatga cttatgatga tacatcttat gaggaacaac cagatagcgc tcattcttcc
    15781 aggatccaac gctatcattg ataggcacaa aatacgagag aggctgtgac aaccgtcgca
    15841 gccttttttg gtttattgca attgtctagc atcttctgta aaatagaaac gttagagaaa
    15901 atcttgtatc atagaaaatt aagaaagaaa taagaacgca gctcggtacc aaaaatatgg
    15961 taaaatggca cttgactgta attcactgaa aatcgcttgg aggaacacaa atgtttaaag
    16021 atttgtattt atttatgaag gacatttatc agaacagaaa attactgcta cagttttcga
    16081 tcaatgactt caaatcaaga tatgcaggat cgtttttagg aatcttatgg gcgtttatca
    16141 atccgctatt tactgtattg atttattggc tggtatttgg tgttggactg aaagcacaaa
    16201 tgatcgatgc aaaataccca tttatcgtat atttagttac cgggatgatt ccctggttct
    16261 tcttctcaga tgcatttgca tcaactacgg ttgtttttcg ggaatatact tatttagtaa
    16321 agaaagtcgt attcaatatt cgtatcttgc ccacgacgaa aatcctatcc aatctgtaca
    16381 cccatttatt cttcattctg attggcatag ctgtcgcgct tataaatggt atatatcctt
    16441 cagcaatgtc tttacagctg atttattatt tattttgcat gtgtgctttc ttgacaggat
    16501 tgacttggct gacggcatcg atccaaccat ttttacctga tatcatgcaa ttaattacga
    16561 tcgtgctgca gttgatcatg tggacattac cgatattatg gagccctaat cagtttgatt
    16621 caggtcttgt aagcatattg aaattaaatc cgctgtacta tgttgttcaa ggttatcgtg
    16681 agtcattttt aagtgaagct tggttctggg agcatggaac atatactttg tatttctggg
    16741 ttttcacgtt agtaatgttc gtaatcggct ctgcagtgtt ccgtagattg aaaccacatt
    16801 tttcagatgt gctgtaagga gaatagctat gacagaatat gcaattagac taaaagatat
    16861 cacaaaatct tataatatgt acgcaaaacc ttcggatcga tttcgtgaag ccctcaatcc
    16921 ttttaagaaa tcctatcatg atgtatttta tgcgttgaaa aacgtgaatg tagaaataaa
    16981 aaaaggtgag atgatcggtt tcgtgggaga aaacggatcg ggaaaatcta caatgttgaa
    17041 aatcattacg ggagtactga caccaacatc cggcacgatg gaaatcgaag gaaaaatctc
    17101 agctttgcta gaactaggat caggattcaa tcctgagtat tctggctatg aaaatatcta
    17161 tttaaatgga atggttctag gattttcaag agaagaagtc gatgaaatgg tcgatgatat
    17221 cattgaattt gctgacatcg gcgaccatat ttatcaacct gtaaaaacct attcaagtgg
    17281 gatgttcgtt cgtctagctt ttgctgtagc gatcaatgtg aatcctgata ttttgattgt
    17341 ggatgaagca ttggcagtag gagatcttga atttcaacta aaatgtatgg aaaaatttac
    17401 agaaatcaag aactcgggaa agacgatttt attcgtttct catgatatta gttcgattcg
    17461 ccgtttttgt gatcgttcct attggttgaa aaatggaaaa gttgtcgaat atggtgatac
    17521 gatggaagta acgacgaatt acgaaaactt tttgaagaaa aaatcagtta aaacagtaga
    17581 tcgaaataaa aaccaagtag aacgcttcgc agctccagat atcgtagacg tgacgaaagc
    17641 cgaattgttg gataaagata aaaatccagt ggatatgctg gaacaaggag aaaaactata
    17701 cgttaaagta acctatgaag taaaagacga tacaatcaaa aatccagttt taggaattgc
    17761 tctgcgtaca gtcgacaatt tttatgtgtg cggactcaat actctactag atggtatcaa
    17821 gatcccttgg aaaaaaggtg agaatatctt ttatctggag tatgaaaagt taggtttgtt
    17881 agctggtgaa tattattttg acgttgcggt ctttgaagaa aatgcaacgg ttcctttggt
    17941 atacaagaca aaatacatga acttgtttgt cagcggttct tatatcggag aaggcatcgt
    18001 ggtcttagac cataaatggg aagaagggac tcatagcaat gaaatatgat tttgaaatgg
    18061 acctagatga acaaagcagt gtgggaaaaa ttgccgcaca gatcaaacca ggaagtaaag
    18121 ttttagagtt tggtcctgga aacggccgat taacgaaaca tttgatcgga gcaaaacaat
    18181 gtgaagtgag cattgtcgag ctagataagg aattgttcga ttttgtcagc gagtttgccc
    18241 aagatggatt ttatggagat atcgaaagtt ttgaatgggc aaactattat gctggacaaa
    18301 catttgatta cgttttgttt gccgatgttt tagaacattt ggtagatcct ggaaaaacgt
    18361 tgaagaaagt tagagaattt ttgaatgaag aaggagaaat tctaattaca ttccctaacc
    18421 ttgcgcataa ttctgtcatg attgatttgt tcaataatca attgccatgg gcttcttatg
    18481 gtttgctaga tgaaacccat aattcttttt atactcatga tggcttccaa aaagtttttg
    18541 aaaaagcagg attatacatc aatattgaag attatttgta cttagctgta ggtgatacag
    18601 aattaaaatc aacttatgaa gaattgccgg aagctgtacg ttatgatttc aaaatgcgac
    18661 catttggcga agtctaccaa tacttcttct cattgatgaa acatccagta gcacaaagtt
    18721 ccattgcgca gccgcaaaat tctaattatg tgaaagtatt agaagtaatc cagcaaacga
    18781 aacaagatga aataattcaa caaataccat tcaataattt cacaggggaa aatgagacac
    18841 taagtttcca tgtatcagaa ttagcagaac gaatggtgtt tcgcttcgcg cagcaaccta
    18901 gctttatcga attcagcgca gaagctgctg gagaaaaact cacatttatc gattcaaatg
    18961 ctgtagtcaa aacagtaaat gattgttatt tgtttgatgg taaagattta ccagagttct
    19021 ttttaacgga tattgcaggg aaagaagtga ctatccattg tcactatcgt tttatcggag
    19081 aattgacacc aacaatgaaa gaattgctgg aaacaatccg tccaatggct gaagttacaa
    19141 agcagctttc tgagaaaaat gatgaattag aaagagaaaa tcattatctt attgagaata
    19201 atacaagatt agatcagacg ttgaaaacaa ccataaatcg ctattgcacg ttgttggaaa
    19261 gtgatgaatg gacaatcaaa catagattag ggcgacgtaa gaaagaaaca tcgaaaaaaa
    19321 tccaagaaaa agagctttct ctatgtattg acgaaaaaat ctgggatgca gaaacaaaga
    19381 tattgaaaat catcgggtgg ggaatcgcta attcggatcg ccaaccattg tcctataagt
    19441 tatcagccga ccaatcacca ttttttgaag caattcctgt tttacgagaa gaagtcaatc
    19501 aagcaaaaca gctagctgaa ggagcaaaag caggatttga actgcggatc ctttgcgaga
    19561 aggaacgttc attcttagtt gaagcagtgg ctcaaaatgg tcaatcatgg atcattgaaa
    19621 tgtaaataat gattaactta taaagataag gagtaaacga atcgtgcaaa aaaaagaaat
    19681 tgccgtattt atcgatcaaa tcactcgtga acgggccact ggcgatttgc ttattgtggg
    19741 ctgggcaatt gatgaagtga ccaaagaaat ccctacgata aaggtagaaa aagaaaatgt
    19801 gattgctgaa gcaacacatg tcgtaagatt ggatatcaat catttatata atcttgatgt
    19861 taagacccaa agcggtttta aggtacgttt atcagggaaa atgagaggaa aagcaatctt
    19921 agacttccaa acagcaagac atcaaaatgg catagctgtg aaattaaacg gtaaatatcc
    19981 ttatgatgac ggcattgaat cttcatggga aagaaaaaaa agattactga aaaaagggat
    20041 aaattatgca aggactcatg gcgtaaaaaa agcaatccgc cgggtcaaac tggaattgaa
    20101 accaggatcg atcgactatg cagagtggat cagtcgtcat gagaaacctg atctgaaaga
    20161 gcaaaggaaa gaagtacaag gatttgccta ccgcccattg atttccatac ttatgcctgt
    20221 ctataacgtt gaaatcaaat ggctcgaaaa atgcattgat tcggtacttg atcaaacgta
    20281 tgatcactgg gaactatgta tcagtgatga tgcttctact gatccagcta tccgaaagtg
    20341 tctagaatcc tatcaagcaa aagatgatag aattaaagtc gttttccgac aagaaaatgg
    20401 acacatcagt ctggcaacta attctgcatt gaaaatggca gaaggagagt ttatagcgct
    20461 cttagataat gatgacgaat tacctccatt tgccttatat gaagtggcaa aagtgctaaa
    20521 cgtacatcca gaactggatt tgatctacag tgacgaagat aaaatcgatg cagatgggaa
    20581 tcgctttgac ccgcacttca aagcagactg gtctcccgat acattgatgg gaaataatta
    20641 tatctctcac ttaggcgtat atcggacaag cattgttaag gaacttggcg gtttccgtaa
    20701 aggatatgaa ggctctcaag attatgattt ggttcttcgt gtgacagaac aaattcctgc
    20761 agaacacatt taccacattg accgtgtctt gtatcattgg cggacgatcc cgggatcaac
    20821 tgcaagcaat ggagaagcta aaagctacat ctatgattct ggagtgaaag cattgacaga
    20881 tgctctatct agaagaaata tcaagggaag cgtacatcct ggcagaattt ctggttttta
    20941 tgaaatcact tatgatgttt tgcaagaaga cttggtcagt gtgatcattc caactaagaa
    21001 tggctatgaa gatctaaaaa cttgtgtgga ttcgatcatc gaaaaaacgt cttatccaaa
    21061 ttatgaaatc attattgcag ataatggaag tacagatcct aaaatgcaag aattatttgc
    21121 agaatacaag catcagctga aagaccgatt tatcgtcgaa ttaattgaca ttccattcaa
    21181 ttattcacgt atcaacaatc tggcagcaga aaaagcaaac ggcaaatact ttttattctt
    21241 gaataacgat acagaagtga ttgaacctga ctggatgaca gcgatggttt cttatgcaca
    21301 gtttgatcga atcggctgtg taggagcaaa attattttat ccggatgata caacgcagca
    21361 tgcaggtgta ttattaggga tcggtggcgt tgcaggacat gcattgaata actatgatcg
    21421 aacacattgt ggttactttg gccgattggt cattgacgtc aattacttag cagtcactgc
    21481 agcttgtatg atggtcaagg cagctgactt caatgctgtc aatggatttg atgaaatatt
    21541 agaagtagca ttcaacgatg ttgacttgtg cttgaaagtt tatgaactgg gtcgttacaa
    21601 tgtctatgca catcaagcag aattgtacca tttcgaatct aaatcaagag gttatgaaga
    21661 tacaccagaa aaacaaagac gttttgctgg ggaaatcaaa aaaatgcaag ataagtggcc
    21721 ttcttatatt gctcatgacc cattttataa cgataatcta actaaagaag gaatcggcga
    21781 tttttcgttg agacctgatt gattaaaaga actgtatttt catttttatt aatgaaaata
    21841 cagttctttt aatttaatt
//
LOCUS       DAXWLB010000032        21739 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXWLB010000032 DAXWLB010000000
VERSION     DAXWLB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.032"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(183..1082)
                     /locus_tag="ACGE4H_000947"
     CDS             complement(183..1082)
                     /locus_tag="ACGE4H_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8364383.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1092..1448)
                     /locus_tag="ACGE4H_000948"
     CDS             complement(1092..1448)
                     /locus_tag="ACGE4H_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8364384.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1804..2100
                     /locus_tag="ACGE4H_000949"
     CDS             1804..2100
                     /locus_tag="ACGE4H_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFF8364385.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2744..3697)
                     /locus_tag="ACGE4H_000950"
     CDS             complement(2744..3697)
                     /locus_tag="ACGE4H_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8364386.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3720..4475)
                     /locus_tag="ACGE4H_000951"
     CDS             complement(3720..4475)
                     /locus_tag="ACGE4H_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364387.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4472..5431)
                     /locus_tag="ACGE4H_000952"
     CDS             complement(4472..5431)
                     /locus_tag="ACGE4H_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8364388.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5424..6374)
                     /locus_tag="ACGE4H_000953"
     CDS             complement(5424..6374)
                     /locus_tag="ACGE4H_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8364389.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6524..6976
                     /locus_tag="ACGE4H_000954"
     CDS             6524..6976
                     /locus_tag="ACGE4H_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFF8364390.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(7064..9331)
                     /gene="gshAB"
                     /locus_tag="ACGE4H_000955"
     CDS             complement(7064..9331)
                     /gene="gshAB"
                     /locus_tag="ACGE4H_000955"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFF8364391.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWKEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9518..10516)
                     /gene="ftsY"
                     /locus_tag="ACGE4H_000956"
     CDS             complement(9518..10516)
                     /gene="ftsY"
                     /locus_tag="ACGE4H_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFF8364392.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10528..11337)
                     /locus_tag="ACGE4H_000957"
     CDS             complement(10528..11337)
                     /locus_tag="ACGE4H_000957"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8364393.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11346..14927)
                     /gene="smc"
                     /locus_tag="ACGE4H_000958"
     CDS             complement(11346..14927)
                     /gene="smc"
                     /locus_tag="ACGE4H_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFF8364394.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14947..15633)
                     /gene="rnc"
                     /locus_tag="ACGE4H_000959"
     CDS             complement(14947..15633)
                     /gene="rnc"
                     /locus_tag="ACGE4H_000959"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFF8364395.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15981..17762)
                     /locus_tag="ACGE4H_000960"
     CDS             complement(15981..17762)
                     /locus_tag="ACGE4H_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8364396.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17788..18702)
                     /locus_tag="ACGE4H_000961"
     CDS             complement(17788..18702)
                     /locus_tag="ACGE4H_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364397.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18729..19691)
                     /gene="opp4B"
                     /locus_tag="ACGE4H_000962"
     CDS             complement(18729..19691)
                     /gene="opp4B"
                     /locus_tag="ACGE4H_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8364398.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19691..20635)
                     /locus_tag="ACGE4H_000963"
     CDS             complement(19691..20635)
                     /locus_tag="ACGE4H_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364399.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20636..21640)
                     /locus_tag="ACGE4H_000964"
     CDS             complement(20636..21640)
                     /locus_tag="ACGE4H_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364400.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6074 a         4661 c         3850 g         7154 t
ORIGIN      
        1 gttcaaaaaa agaataaacg cttgcatcta gtactctgcg agttacaaat catccgatca
       61 aataaacatt cgctgattat tttccacaaa aaaagcagca tatccacgat aaatggatct
      121 actgctctta cttatcctta tgttttacaa ggtattttct tttacacatt actaatgctg
      181 tctcaatacg gaacgattgt catcgcctgt tcaaattcct cttgcaaatg ttgtgtttga
      241 taggcttgaa aggccggatg atgggcacgc aagaggacac gtaatttcat gggttcatag
      301 ctatctaaca gctgttcttt caagacaaaa tctcgggtca gctgccattc ataataataa
      361 ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa gcgacgatgc
      421 gtatggccaa aaactacttg tttgatattt gaataacggt caagtaattc tccgaatgat
      481 tctgatccta aaaatgcatt taatttgttc cagcgctcat aaggagcaga ttgatacatg
      541 ataaatgccc gtttaggaac gaaatgagtg gataaaatca cctcaagttt ttttgcttcc
      601 agctggtcta gcaaattttt tgcagctgca agcatttctg cattgacctc gagatctgtt
      661 ttcccacgct ggatgaaccg atcataccaa tacaaacgct tcattcgtac gattgcctcg
      721 taatctttca attcggaaaa ctgatagtcg taccagccat tcatccctaa aagaacggtc
      781 tgctcattta aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa atggtccatc
      841 atctgttcat tgggcacatc tgccaactcg tgattgccga aatgaaatgt cgtagataat
      901 cctttattga cgaaaaattc gacaacagct aggcaacggt ctactttatt agccgtatct
      961 ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag ctgcatcagc
     1021 tctgattcgc cgaatttctt gatgtccaca tgtaggtcgc tgatgatcgc caattttccc
     1081 attttatccc tctagtcttt tcgaacaact tgcaagttgc cttttctcaa gccgcttggt
     1141 ccttttaatt gtaaactatt ataagttggt tctaaagcaa gtgtggatgg atcatccatg
     1201 taaagtattg cactgtcttt gattttgatt ggtccaactg gtgtatcagc cactacagaa
     1261 taaagactta gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa tgccgtgtcc
     1321 aattggcaac tgacttggct ttctgcaaat ggaccgtcac cgttttcgat atctaataaa
     1381 agttgatcgt ctgcttctaa tttattcttc aatacttctt gtgcttcttt tgtcacttct
     1441 aatttcatct gtatcacctc atgtcataga ataatcactt tattcatcag atgcaactaa
     1501 tttgtctggt tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt tattatgatt
     1561 tctcttatca caaaagagac aactttccct tttcttcgtt cagtttcttg aacggaaaaa
     1621 gacccaaaaa gcaacctgtt atttgtttaa ccttattcct ttcttaaata acaaaaaata
     1681 ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga
     1741 ttattaacaa ctcttcggta gagtaaactt atcaaataaa aagacttctt tggaggaatc
     1801 actatgaagg aatatataaa aaacatctat tttatcgaag aaacacaaaa tatcgagggt
     1861 tcttatattg aagtaaaaac actctttgtc aatgaagata aaaccaaagc attggatatt
     1921 tataaaaaat tagcttcaaa aaaaacgaat agttttggat tgatattaag cgagtataaa
     1981 atcaaagcag aagagagtta tttctatcaa ttattaaaac ggtggagtaa gcttcccgcc
     2041 gatttttatc gaaaaatgca gatcatcaac tatcaaccgc ttgctgagac acatgcctaa
     2101 atagaggttg tgaaatcagt gctttgagct aataccgaag gtcagctgat ggaacatcgt
     2161 ttagaagaga ttgtgacaac tacgatttgc tttaagtaat aagaacgctg gacagaaaat
     2221 agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg
     2281 tttagaagag attgtgacaa ctgcgatttg ctttgagtaa taagaacgct ggacagaaaa
     2341 tagcttctca tattttctag tctagtggac ttattgccga gaagcagcag atggaacatc
     2401 gtttaaagaa gagatcggta caaaataaaa atatctaaat ttaagtttta tctaagtttc
     2461 gctttgtacc tttgattcaa atcaaaggag gcgatcttga tgctgaaaaa atggatcaca
     2521 ctattttttg ccactcccca ttctagaatc aacccgtatg atgtagagaa tgaattcacg
     2581 ttgaaaaaag atttcccaaa taaataaaaa ccatccgtaa aatcatcaat acgattttac
     2641 ggatggtttt tgataacttc tttgaggata ggctcttgct atttaagaat gtaagaaaca
     2701 ttattgttac atagtatgag agtttaatcc tctgttattt atgctattta aatgcttgat
     2761 tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc cccgcttaaa taccagacat
     2821 ctggttcaag ggagatgatc tgctgatttt taccagcatt cgtttgtgct accagttcat
     2881 tggcagaaat atcatctttg ctgtcgtctc caccaatagc ttttgttctg tctactacaa
     2941 ataagatatc cggatttttt tccagcacat attcataaga aacactttga ccgtgagtgg
     3001 atgcttcgat ttgatcatct gcttgttcaa agccaaataa atcatggatg aaaccgaatc
     3061 tagaaccaga tccgtaggct gataattgcc cttcattcac aagtgtgact agtgcttttt
     3121 tgtcagtagc ttctgctttc tttttcgtct gatcgattgc actagataat tcctctaatt
     3181 gagttttagc ttcttcttgc ttaccaaaga tctcccctaa agcagtgaca ttttgctgga
     3241 aagattccca tggatttttc gcatctaaag aaaggaaaat ggtcggagca atttgactta
     3301 gatctttttg aaaatctctt tgccggccag aaatgatgat caggtctggc tgaagctgat
     3361 tgattttttc taagtctggt tctttgatcc cacctgctga ttccactttt ttataggatg
     3421 aaagatactc aggaaggctg cttgtagctg ctccaactac cttatctcca acaccaagag
     3481 catccatcgt atctaaagaa ccattgtcga atacgacgac tttttcagga ttctttggca
     3541 cctcgatttg atcgccatta ctgtctttta tcgttaatgc cgtttcagct tgcgtactgc
     3601 ttttctttgt atctgcctga ctggtgttgt cactatttcc acatgctgct aaaccaaatg
     3661 atgctagaat caagatacct actactaatt ttttcatctc ttctctccta atgttagttt
     3721 tagaaataaa gacagaatct gcgaccttca atctcacaaa ttcgtatgtc catttcgtac
     3781 aattcgtcca gtacatcttt ttttatcatt tcttgggtcg ttccttgcgt gaataactgc
     3841 ccatctttca ttgcaataat ttcgtctgca taacttgcag caaagttgat atcatgaaga
     3901 acaataacaa ttgttttccc gatttcttgt actaattttt ttaagatctg catgatttga
     3961 accgcaaaat tcatatctag attgttcaaa ggttcatcca gtaaaatata atccgtgtct
     4021 tgcgctagca ccattgcaat atacgcacgt tggagttgac cgcctgaaag agtgtcaatc
     4081 gattgatccg ctatttcttc taacccaagg tgttccagtg attcctgaac gatttcctcg
     4141 tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc caaattctac taattctttc
     4201 acggtgattt ggacatttat ttcattcgat tgtcgtagga tcgataattt tttggctaat
     4261 tcattggatt tccaagcctt tacttcgtta tggtctaaat agatttcacc tgtatctttt
     4321 ggtatcaaac gactgatcat cgacaacacc gttgactttc cagcaccatt tggaccgata
     4381 aaagcagtca acttcttttc aggaatcgtt aactgtacat ctttaaccac tgattttcca
     4441 tggtacagtt tattgacctt ttgtaaatca atcataggat actcttcctt tcgcgaatca
     4501 attttgtgac aaaataaatt cctccgacaa attcaacgac gacactcact gttgtattcc
     4561 agccgaaaat ctgctctact gcgagctgtc ctcctgtcag aaacagcata gctaccagaa
     4621 agcttccgat aaatagctga ccatgacgat aagtagaaaa gacacgataa ctgatatttg
     4681 cgacaataaa ccctaaaaaa gtaactggac ccacaagagc cgttgatgtc cctgtcaaaa
     4741 gactgacaca acataaaatc aaaaattcaa aacgatctgt gtctatcccg aggtttactg
     4801 cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg atatcgaaat aacaggtacc
     4861 agcagccgat aatgatcaaa gaagcaggca aaagatactg actctggata ttgccaaagc
     4921 ttgcaaataa tctgccttga agcaaatcat attcattcgg atccatgatg acttgtaaaa
     4981 aggtactgac actaccaaaa agcgtaccta aaatcatccc tgacatcagt aataaaaaaa
     5041 gattcgctct catttttttc aaaagaaaga aaaataacaa tagactcagc ccgaccatca
     5101 gcccaacgtt caataagaaa aaaactgtac ctgtctgctg aagcatcgtc actcctccga
     5161 taaagaagaa aaggaccgtt tggacaagga catataacga ctcaagtccc aatactgaag
     5221 gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat tgtggcactg ctgacagcta
     5281 gcgagacaag gataaatgca aaaattttct tactcctcaa tgcccatgca aaatgccagt
     5341 ttccatatgt atggatcgtc aagtacattc ctgtaacaaa taaaacaaga acaagcatac
     5401 caataaaaat agatggcttt tttttaagca tatcgtcgcc cccttatcag aagataaagg
     5461 aagatagccc caccgatgat tccaacgatc acactgactg gtatttcata aggaaaaatc
     5521 aacacgcgag cgacaagatc gcaaaatact aagaacaaac tacctgctaa agcagttacg
     5581 ccaattgttt tgtgcacttg atctccatat attctagaga caagattcgg aatgatcact
     5641 ccgataaaag gaatgcttcc taccatgatc aacatgactg cactagccaa tgatactaag
     5701 ccaatgccta aaaactgcat cttctggtaa tctattccca aatttactgc aaacgattcg
     5761 cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa ccaagagcaa caatggaaca
     5821 acagcataca ataactcata atttcctcgt atcacagtcg caaaatttcc ttgaagccat
     5881 gaagtcatat tttgtaccaa ctggaattga taagcaaaaa aagtagcaac tgaaccaatg
     5941 atattcccta acatgactcc aatcaacgga agcatcaatt gatttttctg ggggaacaat
     6001 cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa agcaaaatgc tgtcaaggat
     6061 cggacaatca aggatgcacc aggtaggaaa atcatagcca ctaaaatacc taaacgcgca
     6121 ctatccatcg tgcccgcagt cgttggtgaa acaaatttat tttgggtcaa atgttgcatg
     6181 atcaacccac aaatactact ggtcgctccc acaaggatca aacaaatcgt tcgtggcaaa
     6241 cgagtcacta gcaagatttg taagctttgc ccatctaact ggagcagttc tgacaattgt
     6301 acattcgata ctccgatgaa caatgaaata ctacttaaaa taactaataa tgctactagt
     6361 cgtccgtagc gcatccaatc cacctttcta attgagaatg attttcattc ttattttaag
     6421 tgaatcgttg aacaaaagca accgcttttt catatgtttt aggtattatt gggaatatgc
     6481 tataatcgta agaaagacaa agagaagaag gagggtattt tttatggcac aagatgatgt
     6541 aaaaagccat ttggataagg gactttatgg tacaccttta gtgaaccctg aagaacagca
     6601 taaatattta gggacattca gggaacgctg ctatcttagt atgactgttg ctgaaatgat
     6661 ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa aaacatccag atgcttccat
     6721 actcttgaat ggggctctac ccctttctgt ccaaaaccaa tatatccagc tagcaacgaa
     6781 aaacaatagc cgatttactg tagtcaacga ttacgtcact gcaaatcctg aggaattcgg
     6841 attgctctta acagcaaaag aagcagtaaa tgaacctgtg attgatatcg aaaaaaaata
     6901 cccagaagaa aactcttcgc cagttgaaaa agaaaaacca aaaaagaaaa aaagcttctg
     6961 ggatcgttta ttttaaaacg aaaaaacaca taaattttta catgcaaaaa agcttttgat
     7021 cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat
     7081 ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaag ggataaacat
     7141 gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg
     7201 taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg
     7261 cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga
     7321 tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt
     7381 ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta
     7441 attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt
     7501 cttcaacaag ttctttcact gttcggatac cgtcaccaat aacattagca gggactcgca
     7561 acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa
     7621 attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat
     7681 cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag
     7741 gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg
     7801 atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta
     7861 ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat
     7921 ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta
     7981 atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt
     8041 ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat
     8101 gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg
     8161 ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag
     8221 aatccaacca atccaattca accagcattt gcttcatccc ttcaagaatt tctctacctt
     8281 cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg
     8341 taccttctgc tacccattgg tcacagtctt ctttttcttc tttccataac atgtacatca
     8401 ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca taaggattca
     8461 aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct
     8521 ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt
     8581 cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat
     8641 ttggatgatt cgtatagccc agcgcactgt tacgaatact gcgaacaggt tcttgcggat
     8701 gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga
     8761 tcatccagcg atagcgcata aagtttctcg ccgttttcaa ataaagttct gttttgaatt
     8821 cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa
     8881 attcaaaatt gaaatggata ccgcttacca tctgttttcg cttcccgtat tcttttgcta
     8941 aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg atcgcttcgt
     9001 ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag
     9061 aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg
     9121 taacaggagt gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat
     9181 aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa
     9241 cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga
     9301 catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa
     9361 ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt
     9421 ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct
     9481 gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt
     9541 tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac
     9601 caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc
     9661 cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga
     9721 attgttttgc ttgaaccatc gcgttttgac cagttgttgc atctagtaca agaagtgttt
     9781 cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca
     9841 tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct
     9901 ctatctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgccg cctgcattgc
     9961 cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag
    10021 cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg
    10081 caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg
    10141 ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga
    10201 gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga
    10261 gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa cctacatctg
    10321 caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg
    10381 caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt
    10441 acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt
    10501 tatcaaaaaa tcccatttga ctccccccta ctctaataca aatttttgga tcactttggc
    10561 tacgccattt tcttcattgc tggctgtcac gaaatctgct gcttctttga cgaaaggcac
    10621 agcattctcc atcgctacgc ctagtccagc gtattggatc atcggtaaat cattttcttc
    10681 atcaccgatt gccatgattt cttcttgttt caaaccaaga tcttttgcca ataaagaaat
    10741 accataagct tttgtaacac cttttggcat aaattccaaa agcatacttc tcgttttgat
    10801 gatctcatac atttcatgga attctgctgg tatttctttg attttaggat ctaattcttc
    10861 ttggtcataa gcgattactg ccttattaag cacaaattca tctgtaatat cgcttatatc
    10921 cgctggttgg aattgcagca gcttattcaa cacattgtac aaggattgct tccttggggc
    10981 actaggcagc tgtaaaacca cattgtcaga caaaacatct agtggcagat ccaattgctg
    11041 agccagtttg tataagcgtt ggatatctga tacttccatc actttctttt ccatgatttc
    11101 accagtatca tttttttgta ccaaaccgcc gttgaaagtg atgctgtagt caccttcatc
    11161 taccagtcct aattcttgca gataatgcac catcgcagct aaggggcgtc ctgtacatag
    11221 aacgatcttg accccttgct gttttgcttg tgctaatgct tgtttattct catctgaaat
    11281 cagcttttgt tcatttaata atgtaccgtc caaatcgatt gctactaatt tgatcatttt
    11341 cgttcctaca ctttcgtttc gttgattttc gcctcttcat tgatatcttc catccgtaca
    11401 gaaacgattt ttgatacacc ggattcctgc atcgtcactc cgtacagtac atctgctgcg
    11461 accatcgttc ctttgcggtg tgtgactacg ataaattgtg tgtcatcttg gaaatcgctc
    11521 aagtaacgac caaaacgttt cacgttggct tcatctaacg ctgcttctac ctcatctaag
    11581 atacaaaacg gaacaggaca gacacggata atagaaaaga gcaacgcgat ggctgtaagt
    11641 gctcgttctc ctccagaaag caaacttaaa ctttgcagtt ttttgccagg aggttgtact
    11701 tcaatctcaa tacctgtttt cagcaaatca gttggttctg tcagtactaa ttcagcttta
    11761 ccgccgccaa acatatttgg aaagacaatt ttgaattctt gacggatagc ttcaaatact
    11821 tctttgaatc ttgtccgcac ttcatcatcc atctcatcca tcgtttcaaa caattggttt
    11881 ttcgctgcta acagatcatc tcgctgtgtt gctaagaatg tatgtctttc actgacttgt
    11941 tcgtattgtt cgattgaatt taagtttact ggtcccagtt tttcaatctg ttcttttaag
    12001 gatgaaacct ttgttctact gtcttcgata tcggttgttt cttgatagtc ttgggaagct
    12061 ttctcaaatg tcagctgata ttctgactgc agataggtca aatgattatc taaaatcatt
    12121 tcagctcggt ctttttggac ttctaatttg gattgttccg acagtcgtgc tttttgctca
    12181 cgattacgct ctgccaagac tgcttcaagc ttatcgatct ccttttgcct tctatctcgc
    12241 tgctctttgg cttttgccaa ctcggctttc aatgtttctc gctgagcaga taattcgtta
    12301 atttgttttt ccaaactttc ttctgtgact tcatgatcag aaaagtctgc tgtcaatgtc
    12361 gccaactgct tttctatcgc ttcttgacgt tcggttgcct ctgctttttg gactcgggca
    12421 ccgcggagct ggatctgcag atgattaaac tgctctttca agacagcaag atctgcttgt
    12481 tcttgtgctt tttgactttg tacttgtgct cttctggctt ccatctggtc gctttcttga
    12541 cttaacgatt tgatttcttc gtcgattttt tgtcgttgcg attcaagatc tgtttgtttg
    12601 tctttcaatt cgttcgtttg cttttcgtaa tcttcgataa actgctgcag ttcacgtgtc
    12661 tcaaaagttg agatttgctt ttctttttca aaacgttcaa gttcatttat gatgttttgc
    12721 agctgatttg tcgcttcttg ttcttcaaaa cgtaattgtt cgcctcgtgt acgcaaaact
    12781 tcttgttctt cggctaaacg agctgtttct tgttgcagct cctgaacttt tttttcttgt
    12841 gcttggagtt gtttatctgc ttcttcaaat tcactagtca actgtttcaa ttcttgattt
    12901 tgtacaaaca aactgccttg gtttcctcgt ttattcgctc cacctgtcat ggatcctccg
    12961 gcattcatca catcaccttc taaggaaacc actcgatatt gatagcggat cgtttgcgcg
    13021 attgcattgg cactagttaa gtctttggct aaaaggatcg ttcctagcag gttttgaacc
    13081 acagtttgga tttgatcggg ataactgact tgttcactgg caatccctat aaacccttct
    13141 acagctgctg cttgtgtcaa tatatgagcc ggtaattgtc ttggtttgat tgtcgtcagt
    13201 ggaaggaatg ttgctcttcc tcctctttgc tgtttcaaat acgtgatggc ttgccgagca
    13261 tctttttcat tttcaacgat gacatgctgg gctgccccac ctaaagctgt ttcgatagct
    13321 aacgtgaaat ctgcaggaac atcgatcagt tcagccactg ctccgacaat acccgaaagc
    13381 tgctgtttat gctgcaagac caatcgaacc ccttggtaaa aaccaaaata gttttcttgg
    13441 atttcttgaa ggcttttctt acgagcacgt agctgctgga cttgattcat taattgatac
    13501 attttaggct gttcttttgc taatgcttct tggaactttt tcccgttttg ttggatcatt
    13561 tctaacttct cttttgtttc agttaaagat gattttagct gcgcctgctt ttgtgtcaat
    13621 tcttcttttt gtaagatcaa tcggtctact gatgcctcta cttccgattg cttggctaac
    13681 gtctgcttac tttttgctgt ttcttgtaca tattgacgtt ctagatactt cagttcgttt
    13741 cctacagctg cttgctcttg catcaaatca acatattgat ctcttagttc tgccagtaat
    13801 tctttcgttg atttttggta tttttctaat tcttgctgtg tttttatgag acttgcttca
    13861 gctttttgga tttctgtttc tttttctgct acttctttca acaagctttc ttgaagcttt
    13921 tcaaaatgtt taaccttttt ttgtgcttct gctaatgaag tttgatattc ttgcgagctt
    13981 ttttgtgtat gctttgtccg ctcctgcaaa acatcttttt gtccttctgt ctgcttcagt
    14041 ttttccgaaa gatctaataa tacttgctga tttttttcga tcagacgatc ggcttgcgca
    14101 ttttctttac gctgcttcgc caaaatggat tcttgttctt ggatcgactc agatagtttg
    14161 cctaattcta gattgaattt cgcaagctgt gcttgtttgt tgtcccaatc tttttttgct
    14221 gtcttgattt cggcaaccat caaagagaca tcggtttgag tcagtgtttc ttttagccgt
    14281 aaaaactcct ttgctgcctc actctgggcg gccaaaggag ttaactgttc ttccaattcg
    14341 tggatgatgt cttggacgcg gctgaggttg tcttctgttt caaaaagctt ctgttctgct
    14401 ttctttttcc gctgcttgta tttcaagaca ccagctgctt cttcaaagat cccacgacgg
    14461 tcttccggtt tactactgaa aatcgcttct acttttcctt gagaaatgat tgagaacgat
    14521 tcttttccta atccagagtc taagaacaat tcttggatat cttttaatct gcagctttgt
    14581 ttattgatga aaaactcgct ttccccagtc cgtcgataac ggcgggttac actgatctca
    14641 ttgtactcca atggcagata atgatcacta ttgtctaaaa tcactgtgac ttctgcaata
    14701 ttcagctgct tacgggtatc ggaaccggca aagatgatat ccggcatttt tccgcctcgc
    14761 agacttttag cagattgttc tcctaatacc cagcggatag cttctgtaat attgcttttt
    14821 ccactgccat ttggtccaac cactgctgtg acgctatttt caaagtcaat gactgtcttg
    14881 tctgcaaatg atttaaaacc ggctatttca attcgtttca aatacaaagc tttccctcac
    14941 ttacttttac agtgctgcca atgcattttc cgccgcagct tgttctgcta attttttcga
    15001 ttttccttgt ccagtgccaa tcaattgatc atctacataa acttcgatcc aaaatactcg
    15061 ttcatgagct ggaccttctt cattgatcaa tcggtattcg attgatacat cacctgattt
    15121 ttgcaagact tcttgtagtt ttgttttatg atccatctca tgtgaaaaag cacctgcttt
    15181 gactttagga aaaatcactt tttcgatgaa tgcatgggtc gtgtcgaatc cctggtcaag
    15241 atataacgcg cctaaaaaag cttcgaatag gtcgcaaagc aatgctggtc gcgtacgtcc
    15301 tcctgaattc tcttctccct tgcccaacaa aacatattga tcaaagtgac attctttggc
    15361 aaatttcgac aagctgtctt cgcgaacgat cgcagctcgt gtttttgtca attttccttc
    15421 tggcaaatga ggatataaac ggaataaata ctgagaaacc atcagttcta aaacggcatc
    15481 tcctaaaaat tcaagtcgct cattatccga taattgcaga ttgcgatgct cattcacata
    15541 ggatgaatgg gtaaaagctt gttcaagcaa atttagatcg tgaaaaacga tgttatagtt
    15601 ttcttttagt tcttttgtca gctggttgtc cattttccac attcctttta tggatatttt
    15661 ttcgatactt ccttattata ctggtttttt gtcaaatttg aaatggttcg gtcaaaaggt
    15721 aagtaaagct ttaataatcc tagtgattga cgagaagttg tgaaaaattc gtttagtttc
    15781 gagtatgaag aaaaaatttt gtgaaaatgg ctttccatat ttttgcataa atttgactta
    15841 ataccgagaa actggacttt gaacaccgtt tattggagaa gtcatgaaat cagcgccttg
    15901 acctgaatat tagagaaata aacagaaaaa tatcaaaaaa ccgaaaaagg acatcgccct
    15961 ttttcggtca tttttagtga ttatgaacgt gaatctgctg taacagaaac atttgcccat
    16021 gggtttggta catcataaga ccaatcccag tctttgacac ggtcgttgac tggcaatact
    16081 tcattacgga acaacgttgg aatcgcaaat gcttcttcaa atgcatattg ttgccatttt
    16141 tgatatgctt ctttttggta ctcttcatca aatgctttgc ttgaactgat atcgttgatc
    16201 aattttgtat tttcttctga ttcataacga gtgtagttga acgcagcatt tggtccccat
    16261 agtccacttg gatttggatc agttcctgta ttccaagcag cttggtaaac atcaatttca
    16321 ggatcatcat tttctaattt atcatagaaa gaattgaatt cgatcagacg acctgttgtg
    16381 tattggacat ccaaaccgat ttctttccac tgttggatat agtaatctgc caatggttgc
    16441 gctgtttccc caccggacat cgaagcaaag ttgattgtta gtttatttcc atctttgtct
    16501 tctcttaaac catcgtcatc cacatctttg tagccagcat catctaatag ttttttcgct
    16561 ttgtctagat cttgtgtgaa tcctttcatc tcatcatcat ggtatgactg gaatactgga
    16621 ggaatcaatg aagtcgcatt gcttctcaaa cctgcataga aacgctcacc tactgcatta
    16681 ttatctaatg catagcccat tgcttgacgt aatgatttat cagccatttt tgaatctgga
    16741 ttgtattcca cactctttgt atctgcattc catttaccta acttgaatcc tagataagta
    16801 taagaagttt gttcacggcc taagtttgta tagccagcaa tttctttata ggtatcgtaa
    16861 gtatctgttg gcatttggaa aatcatatca tataatttag agttcaatgc ttcagttgca
    16921 cttgccatcg gtacagattt catcgtgatc tttttcaatt taggcgtacc attccagtag
    16981 tgctcgtttg gcaagtattc tactgattct cctgcaacga tattactcat gtaataaggg
    17041 ccaaaagtca ctggattttt acgtacttga tcgcttgatt ccatatcttt gattgctaca
    17101 ccttctaacg tatgttttgg tgctgcatat gaccaaatac caccacccga ttgaagcatg
    17161 cttgggttta cttctttata ttcgatttca actgttttgt cgtctatttt tttgattccg
    17221 gagattgtgt cggcacttcc atcatgatat tcttccatac ctacgatgtt acggaaagtg
    17281 tcgtcataac ggataccagt gtaatcttta ttcccaatga tttcgtaagg gaaaatcaca
    17341 tcgtccgccg ttacatcttc accatctgac catttgacgt tgtcttttac tttgattgtc
    17401 gcttttttcg tatcttcatc tagatcaagc gttgcagctc cgtcgtctgt gattctgaaa
    17461 tcgtcatcgt ttgcaaatag cggttcatga gaaggagcca taaaagctgc atcatatgaa
    17521 tcttggtaaa actcccactg gaacaatcct ttaaactgac tgtccgttgc gaccgcagct
    17581 tctaattcgc cgtcttcgat tgcatcatca ttattttttg ttgcaattgg aaaagttaat
    17641 tcctctttgt tattgccgtt gcctccagag ttgtttccag aggtatctgt acctccgccc
    17701 ccgcatgctg caagaacgac agcagacatc agcgctgcaa atccaaaaat cgttttcttc
    17761 attcctattt cccccttcgc ttctatacta gcctaaccgt tggcgcgcat ctgcagaacg
    17821 cttgaatgct tgtccaacat aattgatact caacatcaaa actaaaataa ggattgatgc
    17881 gggtaaccag atccattgcc gattcactaa gacttctccg ttacttgcat aaccgatcaa
    17941 tgtacctaaa ctaggtacat ttgccggcaa gccgaaacct aagaaagtca aagttgtctc
    18001 gataccgatg tttgccgcaa agttcatcgt caaattagta atgattaatg aacttagatt
    18061 cggcatgatt tcgcggaaca tgattttaaa atcattggtt cccattgttt ttgaggcact
    18121 gacataatct cttcttgctt cagataatgt cttgcttcgg aataatcggg ctttagctac
    18181 ccaataaaat gcactcatga tgaatataaa ggaccagaca ttgtatctgg aaataatcga
    18241 tacgaagacg ataatgatca gcatgatcgg tagaatcatg ataaaatcaa cgatccgcat
    18301 caagatgttg tcaatgatcc ctccgtagta gccagagatg atccctaatc caaccccgat
    18361 gatcgaagta aggatcgtga tcgcaaatcc gatcgtaaca gagtttctag cacctatgat
    18421 caattgtcct aatacatcac gtccgccttc atctgctccc agaatgaact tttgtccttg
    18481 tgtcgaaact gttcctggtg cagcgtattt gtcgaagata ctaacataca tcactttgtc
    18541 ttgatcggtc agcatcgcac cgataaagat cactaataat aaaaccacta gcaaacctaa
    18601 tgaaaaaata gcaattttat cttttttaaa ttctcgagca atcattcgta tccccatagg
    18661 aggaatactt tcttgaactg ctgtattgtt tttttcactc atcgatccat tctcctttct
    18721 gattggtttt attgaatccg aatacgcggg tcaacgatac tcatgatgat atctgaaagt
    18781 aatgtcccca ctagcgtcgc gattcctaaa atcaatacta aggatgtgat cacggtatag
    18841 tcccgttggc cgatggaatc aataaatagt tttccgactc ccggataacc aaaaatcttt
    18901 tcgataacta cagaaccagc aatcaacgaa gtgatttcat accccatttg agaagccatc
    18961 ggcaacgctg catttcggaa aatatgtcga ctgaatactt tattcgtcgg tacccctttg
    19021 gaacgggctg ttctgacaaa atcgagcgat ttcgaatcga ttacttcact tcgcagatat
    19081 tggattgtga cggcagtcgc caaaaatgct tgggtaattg atggtaacag aatatatttc
    19141 aaacggtcta tccattgagt gataaatcca ccttgcaaac ctagtgtttg tgatccactt
    19201 gttgggaacc agcctaaacg ataaccaaaa acaaatagca tgatcaagcc gaagataaat
    19261 aaaggaacag caaaactaaa gaagttgtag attacgacaa tcttatccaa gattgaattt
    19321 tgataccggc cagcaagcat ccccagaggc acagcaatca aataagtaag gacaactgtc
    19381 aagatcgata gatataaagt gttgatgatc cgttcgccga tcaagtcagc aacagagcgt
    19441 ttgtataaga aactttgacc aaaatcgcct tgcaaagcat ttccgatcca acggaagtac
    19501 tggacatacc atgggtcatt caagccagct gcttgtcgca tctgttcgat cactgccgga
    19561 tcctgatttg gattgatcaa accggtaaat ggatcccccg gcatcgcttg ggctaataag
    19621 aagatcagaa cacttaaaat aataacttgc gggatcatca ataaaacacg tcgcagaatc
    19681 gttttccaca ttagcttgca cctccatctt tcaacgccac tttgtgtgta gttgtgatcg
    19741 tacgcaaatc gtagactcga ccagtcgcat catagtattc tttttggttt tggatatact
    19801 cttgttctac tcttcgtcga ttttctttgt ggctttccct attttctaca tcgatttgcg
    19861 gaatagctga aagcagacgt ttcgtataga tatgtcgcgg gtcattataa atgtcttctc
    19921 ttgtaccgat ttctacaaat cgacctttgt acataattgc gatattgtcg cacatgtgtt
    19981 ttactacacc taaatcatgt gaaatgaata aatagctgag gccgtattct tgctggattc
    20041 gtttcatgaa attcaatact tgcgcttgaa cagatagatc cagagcagat acaggttcat
    20101 cggctacgat cagttttgga ttggtagcaa cagcacgggc tactcctaga cgttgacgtt
    20161 gtccgcctga aaattcatga ggatatttat atagcgcatc ttccggcatc ccaacgatat
    20221 ctagcagtcc ttttaccttt ttcttttctt cttgatcgct taaccgttca aaatttcgga
    20281 tgggttcagc aatgatatcc aacactcgct ttttaggatt caaactggac attgaatctt
    20341 ggaagatcat ctgcacatcc ttgttataac ccattttttt gcggttgctt cgcttcgtta
    20401 catcttttcc ttcatacatg atttgtccgg ctgtcacctt ttctaatccc acaacggctt
    20461 ttcccgtagt agattttcca gagccagatt ctcccactaa accatatgtc tttccttttt
    20521 cgataataaa atccacacca tctacagcat atacatgatc cgttactcga ttgaagaatc
    20581 cactgcgaat agggtaatga actttcaggt ctttgattgt gattaattct gtcatctaca
    20641 cctctccttt ttcgtctcta aagtggaaat gcttatagca ggagcaacgt accaagtggt
    20701 tcggtgcgac ttcatgaagg ataggctctt cttcatgatc gcttgctggt atccatggaa
    20761 tacgtggtgc aaaccgacaa cctttacgag gcatgttttt cagagaagga acgacacctt
    20821 cgatcacatg gagttctgca tcatgatttc cttcttgtgg tatagagttc agtaaagagc
    20881 gtgtataagg atgcttagga tgatcaaaca gttcttttac acctgctact tcaacgaact
    20941 gtccgccata catcactgcc actcgatcag ccatttctgc tacgactcct aagtcatgag
    21001 tgatcaagat gatccctgtt tttgtttctt cctgaaggtc tttcagtaaa tcaagaatct
    21061 gtgcttggat ggttacatct agggctgtcg tcggttcatc tgcaatgacg attggaggtt
    21121 tgcaggcgat tgcgattgcg atgatgacac gttgtctcat accgcctgat agttcatggg
    21181 gatattggcg tccaactctc gcaggattag gaatccctac ttgttctagc aattctagtg
    21241 cacgagcttg acgctgttct gcattcatct ttgtgtgata ggaaagtcct tctttgatct
    21301 ggtcttcgat tctcatgaga ggatttaatg cagacaacgg atcttggaaa atcattccga
    21361 tatcatttcc gcggatttta ttatagagcg tttcgttcag gtcgattaaa ttcagatcgt
    21421 tatataaaat ctcacctgta atttttgtgt tattaggatc aagtaatccc ataatcgtag
    21481 tagccaaggt acttttcccg cacccagatt ccccaacgat tgctaaaatc tcgtttttgt
    21541 ctaaagtaaa tgaaacgtca tctactgcat cgtaatagtc atctttgagt cggaatcctg
    21601 tatgtaggtt ctgaacttct aataattggt tatcggacaa tcgaataccc ctctctgctt
    21661 cctaaatgga agtttttctt aataaaaaag gacattacac agaataaagc gttttataac
    21721 aaaggttatt cttatcttt
//
LOCUS       DAXWLB010000033        21568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXWLB010000033 DAXWLB010000000
VERSION     DAXWLB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.033"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(23..568)
                     /locus_tag="ACGE4H_000965"
     CDS             complement(23..568)
                     /locus_tag="ACGE4H_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8364401.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(580..1272)
                     /locus_tag="ACGE4H_000966"
     CDS             complement(580..1272)
                     /locus_tag="ACGE4H_000966"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFF8364402.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1291..1575)
                     /gene="macP"
                     /locus_tag="ACGE4H_000967"
     CDS             complement(1291..1575)
                     /gene="macP"
                     /locus_tag="ACGE4H_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFF8364403.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1588..2151)
                     /locus_tag="ACGE4H_000968"
     CDS             complement(1588..2151)
                     /locus_tag="ACGE4H_000968"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFF8364404.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2379..3041
                     /locus_tag="ACGE4H_000969"
     CDS             2379..3041
                     /locus_tag="ACGE4H_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFF8364405.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3165..4361
                     /locus_tag="ACGE4H_000970"
     CDS             3165..4361
                     /locus_tag="ACGE4H_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFF8364406.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4462..5838)
                     /locus_tag="ACGE4H_000971"
     CDS             complement(4462..5838)
                     /locus_tag="ACGE4H_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8364407.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5875..6741)
                     /locus_tag="ACGE4H_000972"
     CDS             complement(5875..6741)
                     /locus_tag="ACGE4H_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8364408.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6830..7855)
                     /locus_tag="ACGE4H_000973"
     CDS             complement(6830..7855)
                     /locus_tag="ACGE4H_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8364409.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7873..8547)
                     /locus_tag="ACGE4H_000974"
     CDS             complement(7873..8547)
                     /locus_tag="ACGE4H_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8364410.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8731..9624)
                     /locus_tag="ACGE4H_000975"
     CDS             complement(8731..9624)
                     /locus_tag="ACGE4H_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8364411.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9698..11395)
                     /locus_tag="ACGE4H_000976"
     CDS             complement(9698..11395)
                     /locus_tag="ACGE4H_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFF8364412.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11388..12701)
                     /locus_tag="ACGE4H_000977"
     CDS             complement(11388..12701)
                     /locus_tag="ACGE4H_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8364413.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12701..16970)
                     /locus_tag="ACGE4H_000978"
                     /pseudo
     CDS             complement(12701..16970)
                     /locus_tag="ACGE4H_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
     gene            complement(17267..17785)
                     /locus_tag="ACGE4H_000979"
     CDS             complement(17267..17785)
                     /locus_tag="ACGE4H_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8364414.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(17787..18404)
                     /locus_tag="ACGE4H_000980"
     CDS             complement(17787..18404)
                     /locus_tag="ACGE4H_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8364415.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18401..19255)
                     /locus_tag="ACGE4H_000981"
     CDS             complement(18401..19255)
                     /locus_tag="ACGE4H_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364416.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19252..20163)
                     /locus_tag="ACGE4H_000982"
     CDS             complement(19252..20163)
                     /locus_tag="ACGE4H_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364417.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLTPKWVYYSGGDGK"
     gene            complement(20204..21538)
                     /locus_tag="ACGE4H_000983"
     CDS             complement(20204..21538)
                     /locus_tag="ACGE4H_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364418.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGISDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6290 a         4747 c         3710 g         6821 t
ORIGIN      
        1 tttatcaaat cactttgttt ttttataaaa tttctgcgcc tagtgtggac tcaaaatgtc
       61 tcaatgccca ttcatgtcca acgggatcga aacttgccac ggcatctttg aaaatatgga
      121 gtttgtatcc tagattatac gcatcgactg ctgtatgcaa cacacagata tctgtacata
      181 cgccggtcaa ataaatatct gtgatctggc gttctcgcaa acggatgtct agatctgttc
      241 cactaaaagc ggaataatgc cgtttgtcta tccaataaac attttcctct tgctcatagt
      301 cttggtataa actttttaaa gagccgtaca attcccgtcc acccgttcct atcacattat
      361 gcggaggaaa taagcggttc tccggatgaa aggaatcttt tggatcatgc gcatcaatgg
      421 caaaaacgac aaaatccttt tgatcaataa acttctttgt atgagcagtc agcttttttt
      481 caattacttg tcctgctgtc ccagtagtca gctttccatc ctctgcaaca aaatcatatg
      541 tgtagtcaat cgagatcaat gcgttcatct tagtcctcct tatacgagat gtttgacaaa
      601 ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg ataaattcgt caaagctttg
      661 tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg atcaaaaatg ggatattgaa
      721 ctgacgagct gtttgtccaa cagcggctcc ttccatttcg caagctaatg cttcagggaa
      781 attcgttaag atttctttga ttttatctgg cgagtcgaca aatgtatcac ctgtaacgat
      841 caatccttct tttgcgttca aattcgttgc ttttgctgct ttaaccattt cactgcgcaa
      901 atatgtgctt gcttcataat atagaggcat tcctggtaat tgtcctgggt tgtagccaaa
      961 tcctgttgca tctgcatcaa aataagctac tttgtctgat ataacgatgt cacctacttg
     1021 taagccttca ccgattccgc cagctgaacc tgtattgatc accatgttca cgccgtattg
     1081 ttggatcaac agacttgtgg taatagatgc taagactttt cctataccgg aacgtacgac
     1141 gatcacttcg tggttaccta atgaacctga aataaataaa gcgcctgcgc gttcccatga
     1201 taatggttcg cttaggttct ctctcaaaat ttttacttct tcttccattg cgccgataat
     1261 accgattttc atctttttaa ttccttcttt ctatataaat gcgattgcta aaaaaaccac
     1321 acataacatt aggataacaa taataagaga tttcatcaaa aaagagttcc atttggtcgt
     1381 cttttcccgt tcgctgatcc ttgttttcgt gattggttta ttctttttgc tttctttacg
     1441 ataaaagctg gcaatctttt tttcttcttg ctgataagct gtttctgctt ttcgctgttt
     1501 ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga agttcgcttc gcgtaataag
     1561 cggtcctttg gccataagcc gatccctcta ctttcctttg gtcaatagtt cccagtattg
     1621 aatcgcataa atcgtctttg catcacaaat caactgatct ttcatcgctt gttgcgcctc
     1681 ttcaagagtt agatgataca actccagtac ttcgtcttca tcttgtgcta aaggattctc
     1741 gaccttctct accccttgtg catgataaat atgaaggact tcatttgcaa aacctggtga
     1801 aagatacatc gaagtaagat ggctcagcga cttcgcacga tagccagttt cttcttctaa
     1861 ttctctagct gctgtcattt cgggattttg tccttctcct ggatcgattt ttccagctgg
     1921 aatctccaaa atcacttttt ctaaaggttt tcgaaattgt ttgacaagaa gcagacgatc
     1981 ttgttcgtca aaagcaatga tcccgacacc gccaggatga aataccagtt ctctcttcga
     2041 agttcctcca tctggcaaac gaacttcgtc tacagcaaca tcaataattt ttccgctata
     2101 gatttctttg cgactgattg ttttttcttc aaattgctca ttgttcagca tatcttctcc
     2161 tgctttcttt gtaatcataa ctgctttttt ttcatttttc aatttttctc aaaaactcct
     2221 tattagttta ccatagataa accttagatt ttctctaaat atagaaaatc cttctttagt
     2281 ccgatactct ttatagtagt ccgtatgcta aaataagtat gtaatcgaat aacgaaaaga
     2341 attcaatact caacgataaa tgacatggag ggtttattat gataaaacga aattggccgt
     2401 ggcttctagc attggtcttg ctatttctct ttgtccgaaa caatagtttt tcttttgttc
     2461 taggactttt attgataggt gtagttttag ttttgtgggg actctttggt tcgcgcagga
     2521 aaaagggaaa acaagaagaa attcctgcac tgactgacga acttgaaact cactatgaaa
     2581 aaagcggtat gacagcgaat gaaatttcat tttttcgaca aacaatgaat caaacaaaaa
     2641 atgagatcaa tcaattgcaa atgaatatgc agcagacagc caaacttaaa gcaatcgatc
     2701 tccgccatga tccagtcaaa gcagcgaaag ctttattcaa agaattggtg aaagaaccaa
     2761 accgactgca tgaagcaagt cagtttcttt atacgcatct gccgaatcta gttgatttga
     2821 caaataagta taatgagatc aatgaccatg agatcaaaaa caaacagacc tatgaaaaac
     2881 tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt aatcgcacaa gattatcaaa
     2941 aattcgttgc tgaggatcta gatgatatcg acgtagagat atctgttgcg aaacagagct
     3001 taaagcgtga taataaatat gaaccagaac aaaaggatta actcttttgt tcataaaaga
     3061 agactcggaa cattcagatg atgtctggct gtttcgagta ctttttctaa agtagagaca
     3121 ttcatgtaag aatcgagaac agattcaaca aggaggaatt ttttatggat cataaaccga
     3181 ctgaaacaaa agatgttaca ccagttaacg atacacttga agatttattg aacaacccgt
     3241 tttcaactcc aacagatacg ctgaccactt cacaacaagc ggagattgat cagctccaag
     3301 agcaacagac agctgctcgt ttaattgaca aactaccggc ggaacgccaa gaacaagcta
     3361 aacagctagc agcaaaaatc gatgctaatg atgcacagtc tgtcatcagc tacggatcgg
     3421 ctgcccaagc aaaattaagt gaattctcac aatcgatgtt gaatcatgtg caagcacaag
     3481 atattggtcc tgtaggagat tccttaactg aactgatgta tcgtctccaa gaagccaatc
     3541 ctgacgaact gcgtgccgga gaagggaaca tcttccagcg tgtcttcggg aaggtcaaac
     3601 aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg tgcacaaatt gacaagattt
     3661 cagtgaaatt agataaagaa aaagacggtc tgctaaaaga taatctgatg ttagaacagc
     3721 tttaccagaa aaacaaagac tactttgatg cactgaatat ttatatcgct gctggagaat
     3781 tgaaaatgga agaacttcaa acaacgatca ttccagaagc gatgaaaaga gcagaagaaa
     3841 ctggcgatca gatggatgtt cagattgcca atgattacac gcaattccta gatcgtctgg
     3901 ataaacgtac gcatgatctt cgcttagcta gacagatcac gatccagcaa gcaccacaaa
     3961 tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa gatccaagca tctatcgcta
     4021 cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt gaccttgctt cgtcaaaaag
     4081 atgcagtaac tgctcaacgc caagtctctg aaacaacaaa cgacttattg aagaaaaatt
     4141 cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga aaatgaacgt ggaatcgtgg
     4201 atatcgagac ccttcaaacc actcaaaatg atttgatcga aacaatccaa gagacattgc
     4261 gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga agtagagctt ggacacatgg
     4321 aagaagatct gaaacaaaaa ctgttggatt taacccaata gatcaccttt cgcttgttac
     4381 taaaacaaac gcccaacaac agtttattac aactgttgat gggcgttttt cttatctttc
     4441 ttctaacttc tccattggag tctatttctc attcatcttg ctgtgtccat agccatagcc
     4501 aaaataaatg atcagtccaa taaccattac gataccgaac acgatccatg tcacagtttg
     4561 cagacgaagc atcaatacaa tacaagtcaa gatggagacg attggcaata gcggaacgaa
     4621 tggcactttg aattctccag cttttggttt gccaaacatt tttctcaact tcagtaagcc
     4681 tagactaaca agcatcaaat aggcgagcgc catgatattc gttagttccg ctaatacatt
     4741 caatgggaaa aatccggcaa agaatacagc tgccacacca gcggcataag tcgctttttt
     4801 cggtgtacga tgcttttgat cgatttctgt cataaactta ggaagtaagc catccttgct
     4861 gacagcaaac agtaaacgag caagtgaata catcatcgag atcgttaccg tcagtagcgt
     4921 taaaattgcc cctacagcga tcaccgcgcc cactgttcca tgtcctacat acctcattgc
     4981 aaaagccact gggtcattga ctcctagttc agtatacgga acgattcctg tcaagatcag
     5041 cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa ccaatgattc ctcttggcac
     5101 atctttttgc gggtttttga cttcctcggc tgccattgca acggcatcaa atcccaagaa
     5161 agcaaaaaac acaagagctg caccatctag cacaccggaa aaaccaaaag gcatgaatgg
     5221 ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg ataaacaacg cgatgatacc
     5281 aaatttgaca tataccatgg cattattcaa acgtaatgct ttcttggctt caagcgacac
     5341 gatgtaagtg acgaatatca ctacgagcac cgcaatcaag tcaatgtatg taccattttc
     5401 cggattgtac ccgccagtta atgcttgtgg aagttcggcc cctaaacttt tcaaaaaacc
     5461 ttgcatatag ccagaccatc cggaagcaac agaggaaacc gctagtaaaa attccccgat
     5521 caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat acaacataca gataggcata
     5581 tggcccacca atcactggca ctcgcgaagc aaattctgcg aaacttagtc cagacaaaat
     5641 gacaacgatt gcagctaaga caaatgaaag tgacagtgct ggaccagcat tttgattggc
     5701 cgcaacccct gtcaccacaa atattcccgt accgatgatc gcaccaattc caagcatgat
     5761 caaatcagac gtttttagtt cttttttcat ctcactcgta tgattgagat tgatctcttt
     5821 cttacgaaac aaatccattg aagttcctcc ccattttagg ttggaaaaca gcgattaaac
     5881 tgttttcata taccctaaaa ggataccacc taaaatgact aaaaggcaac caacaataac
     5941 ataaatgagc tcttttttcg tttttctttc gccaaggatg aaaatcccac ctaaagtaga
     6001 gataatgatc cccatttgtg aaagtgagaa gccaacagct aaaccaagtg atttgactgt
     6061 tagaagcatg cagatgtttc ctaagcccca taataatcca cagatcatgt ttttccacac
     6121 aaactgatcg actttaactt tacggaaagc aaagaagctt gcgcctaaga tcattccgat
     6181 actttgaggt aagataattg acatcgcatc caaattcgcc cagttgacga tgatggtata
     6241 tacaccataa ccaatcgtag agaagatcaa tgctctaaaa cccttaccaa aatcaagcat
     6301 tttattatct gtatctattt ttccttcgtc atcttgtcgg gcagtcaaat acgcaccgct
     6361 tacaagaaga gctaaagcaa taataccgta tacataatct ttcgtttgtg tccattcatg
     6421 gaagaaaacg gctcccgcaa tcgtattcaa aattaattga aaaccagtag acaatggtag
     6481 ccctacagaa acacccatgt acttcattcc atggaactgt ccattttgtc caacactcca
     6541 agctaagccg gataaaaatc cgatcaagaa aatccaaggg gtgattacag gactaacaac
     6601 gaaaaagaca actaaagaaa acagaaatgc accgatcgtc attcctagtg tttgttgatt
     6661 ggcatcgcca ccaattttac cacttactaa accaatacta ccccatgcca acatagggac
     6721 taatgctatt aaaatttcca taaccaatct ccttttattt cacatgataa gaaacttatc
     6781 agcgaaaacg ttaacaacac aagcactttt tttcgtttct aacgattttc taacgttttt
     6841 cattataaaa atttaagatt gggaaaaata aagtcattgt tgtccctttt ccttcagttg
     6901 attcagccgt caaatggata cctaattttt cggccagatt ttttgctaaa taaagtccta
     6961 atcctgttga atgtgttttg ctaagtcgac catttctccc agtaaatccc ttatcaaaga
     7021 tccgtttcaa gtcttccgct gggataccga tccctgaatc tttaactgaa aggctgactc
     7081 cttgcgcttc cttttctatg ctcacaatga tgtttccgtg atccggcgta tatttgatgg
     7141 cattactaag caattggttg aagataaaag cgatccattt agcatcggtc aaaacagtat
     7201 gatcctcacc aacgatctcg tagcgaatat ttttttggat aaaataattt gcctgactgc
     7261 gtatcacagg ctgaatgatt ccttttagtc catattcttg gatgaggtag tcccgcgaga
     7321 aactgtcgag tcttgcataa tacaaaactt gttctacgta ttcatcgatt tttgataatt
     7381 cattttctag taatgtatac ttttgatcgt caatatcgaa ctcgatcgaa cgaactagca
     7441 atttagaagc ggaaagtggc actttgattt catggaccca tgagtcaata taatcttttt
     7501 ggtcttgttg attactgatt gctgattgca ttacttcttg gtgttcacgg atcagctgat
     7561 tgatatattc ttctaccagt ttttcttctt ctgtcctccc accactcaaa taattttgca
     7621 aaggggaagt tttttgtatg tcggctaatt ttccccacca tcttctcttg ctcaagtaac
     7681 cacccagcag ataaaaaatc agaaagacgc cctcaatcaa agctaaataa gcaattgtgc
     7741 tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa gatcgtcaat gcaataaaaa
     7801 acagccatcc caagatcaat gtccactgat ctttcaaata tttaaaaaaa ttcatcttaa
     7861 ccgctcatcc tttcagggaa cgatatatcc ctgtcctact tttgtttcga tatatccttg
     7921 gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg ttgactgtca atgtattgtc
     7981 atctacaaat cgttcgtcat cccacagtgc tcgcagcaat ttttcccgac ttaagatctt
     8041 tccattgtgc ttcatcaaaa taaataatag acgatattca tttttgctca aatcaatgat
     8101 ctctccgttg atttccatac ttccgttgtc aacattcaac gtaatgccat tgtggctcat
     8161 catttcacta gataattctg tgtagttgta agaacggcga agtaatgcat tgatttttgc
     8221 aatcagtaca tcgatttgga aatgttttgt cacaaaatca tctgccccca tgttcatagc
     8281 catgatcata tccatattcg tattcctgct ggagataaaa atgatcggta ctttcgatac
     8341 ttcacgtatt ttttgacacc aataatagcc atccaaaacg ggaagattaa tatctagtaa
     8401 tacaagctgt ggttcttctc tttcaaagtc tgagaacact tgattgaaat cggtcgttcc
     8461 aaaggtttca aattgccatt tctcaagttc ctccatgatc aattgtcgaa tgatctcttc
     8521 gtcttcgact accatgattt ttgccataaa aaatccctcc atctttcttc ttgctactac
     8581 aatatcaaag aagcccttgt gaaacaagtt ggcttacctg gaaaatgtca ttttagcaaa
     8641 cgttaaaaaa aactggaaat atgcgattta atcgcgtttg tctcttccaa gcagcttaaa
     8701 tttattatgt tattcttaat aattggtcta ttatctgtta tttttctttc gttgtttttg
     8761 tcttttaaat tttcgataac accaataacc gaaaagcgtt agcactagca atatacctag
     8821 aaggagtata agtaatcgat aaagatggta tttttttgtc tgttctatct ttgaagagac
     8881 ttcttctgta aagggaactc gtcttcctgt cactagtaat cggtgagtgt tcacggtgta
     8941 aggtgtacaa gtgataagtg tgactagatc ctgcttttct ctaattgcta atgtatttgt
     9001 ttttgttgga aggacaacaa ttttctcaac gacttcataa gcgagcatcc gaccatacac
     9061 ttcaagatag aatcggtcat cttgctccat ttcttcaaga tccgtaaaga gttttttctc
     9121 agcaataccg ctatgggcgg taatcacgga atgtgtactc tctcctccag ttggaaaaga
     9181 ggtgccttgc aggactgtcg ctcctctatc taacaaaaga tcattcgttt catcaaataa
     9241 aggcaagcta acattgattt tcggaatata gatggctccg atcatatgtt gttcgtaata
     9301 ttcttttcct ggattttgga catttctaac cgcttgctca aaaggatctt tgacttgtcc
     9361 cattcctggg attactgatg tatgagcaag cgtctgattt ttcttttcat gcactgctaa
     9421 ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc ttttgataat tctcgatcct
     9481 tctttgatcc aaataattgc tgagagcatc tgctaaaaat ggatatgtaa aaactgcagc
     9541 tccggcaaga aacatcactg ccataaataa ttgtaataaa agactgattt gggtttgagg
     9601 tctattacct attttctttt tcattttctt caccttctta tttaaaaaaa ctgcagcaac
     9661 ctttataaaa ccgattgctg cagcttattt ttgttgctta tacttcagac ttcattttag
     9721 atttacggta ccagctgtac gctccaatca tcaaacttat tccgattagt aagaatgcca
     9781 agagcccgtt cccaccagta gcaggaagtg acccgcgttt catattcggt actctttctg
     9841 gccctggatt ttctactcct acgatctgaa tttcttcatt atagcttcca taagagatga
     9901 taaattcagt aaaggctgcc tcacctgttg gaagtacata accttctggc gccatggtct
     9961 ctttcatttg ataggttcca taatctaacc cttgaacctc aaagtatcca tttccatctg
    10021 cgacaaattc tgttgcttgt ccgcttgttg cccactcaat tctatctgct cctgattcat
    10081 aaacagctaa aggcgactcc cctttataaa atattgcgta ttcagaacgg tcttcattcc
    10141 acaattcgaa tcttgcatct ttaagcaatt cactgcttga tgcatcatat ttttcaaact
    10201 ttcttccacc tgttgtgact ttctctggcg gctctactcc ttcttcataa tcaaattcat
    10261 catttcgtcc gatttcaata accgctgtgt tgttatggaa atcatccgct tctagttctg
    10321 tggaaacctt caaattatac gtgatcgtta aatctcctcc acgtgcatct gctaatctac
    10381 ctaaagtatc tactttggct actccatttt cttctgtcaa aatcgtcacg acaaacccgt
    10441 tgcttactac ttctactata taatcttgtc cttttgttaa aatcgtctcg ccggctcgca
    10501 cttctatttc ctgatcataa taagttagac caactgtagg cgtatctgtg attttgtaat
    10561 ttttgataac agcgcttccg ttttcaagga cagaatcgat gaaataagga ataggtgctg
    10621 tgatcgtgta agaagccgtt tttcctcttt ctagatcaat ataagaaatc gtttcttgtt
    10681 ctccagcttc attaataatc gttacttgtc taaggtcgct ttcctctaca ttcatctgtt
    10741 tatctgcttc tctgatttcg tttttcggat acaaatgcac atctgtattg tatgtgtctt
    10801 tccatgtaga accatcccac acgccatcca ccatatcagg catcatcact ggcatcataa
    10861 caaccattgg agacgcgatt ctggaaatat gagctggcga ataagtttct aagaaaagat
    10921 agactttatc tctagaagaa ggtgcttctt ccgtcacttg gacggtagag aataccgctt
    10981 ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag tcgatatttt aaaaaccaac
    11041 tttctgtgtg ccgtttttga atccattcaa tcgcttcggc agaattaaga ccgtcttctc
    11101 gttccggtgt taaaggatta tctcgaatca aatcgtaata tacttccgtt acgtcaaaca
    11161 aatcaaaatc gactcctgga atattttctt caccaaaatc cgtagaaacc aatccgctat
    11221 tcggtcgttc ttccggaaca gatgagaaac cttttttgtg taatgtaatc gtcacttcac
    11281 ttggtttttg tgcgacttct tcggctttga ctagttttcc gttcccgaaa atccctattc
    11341 ccgcagtaac gagtaacagt acactcattc ccgtgacaat cattttttta ttcatttttt
    11401 tctccccgtt ctttttttat taataaaaca gcacctaaca ataaaagaat tcctagtaaa
    11461 gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg accattttcc tgattgacct
    11521 ggattgcttg gaccactcgg ctctttcggt ttgtccgttg tttcttttac ggtctcgtta
    11581 taaacacgtg gtgttgcgga ttcataggca gagactggaa ctttcccgga ttctaattca
    11641 gccatctcct gtccgttaac tactacatat tgtggttggc cttttgcatc cacccaaaat
    11701 tcagggatga tgacctcgat tctcttactt gcttcatcca tctcgtaacc ttccacacct
    11761 tttaattctt caaaataata ggtgccagaa ggcaagaaac gttgtcctgt cgtaacaagg
    11821 ccctctttat ccgaaatgaa tttgcttaca tttttgtcat tcaaaggatc attgtctgct
    11881 ggtttgatcc attgattttt caagtcatta gcaggagcca tatctaaata atatttcccc
    11941 ccttctatca tctgatatag agcaaaaaca gcaccttcta atggttttcc tttttcagtt
    12001 gttcctcttt gacgtccata cttgaagaag tatggatctc ttagataacc gacattttta
    12061 ggatagatat gaatctcttg cagttcctcg gttgattctg tcgggtgata aattggcaaa
    12121 atagcagcaa ttgggatagc tgtctgatcg atatcaacat ccaatccaac ttctggatca
    12181 atcgattctt cgaaaatcaa ataaactgca tcccgatctt ggtttttctt aggtgctttg
    12241 atacgtgcaa tcccgtctcc taatccgcca accgtatctt tcccagttgt taccgaagtc
    12301 cctatttgga caagcctttc ttctgtgatt agctgacgga tattgtttct gttcgtttca
    12361 gcgatttgct ggacgaattc tttaggagtc atgttttcag cagctttttt ttcatgataa
    12421 taatcggtca tgtcgtaaat ggcaaatgtc gctccattta gtggcaccgt ctgtgaaatg
    12481 atatctgctg tttcccctgc ttcctcagag atagccaagc catcattttc atggtattca
    12541 aattgatcgt tataatcgat atctcgaatc atccgtttat gcaacacgaa ttccacgaca
    12601 tcttcccctg attcttctgc cgacatcgca tgtggaagaa ggactgctgc aagcggtagt
    12661 aaaacaagta attgaagcat caaacacaac agctttttca ttaccttgct ccctcctttt
    12721 tagattgtct gacaaacaaa ccataggctg ctcctgcaag cgccatgaag aatgcagcaa
    12781 gcaggatata gaataatcgt ccggatcctc ctgtagcagg caacggattt tcaggatcgt
    12841 tcccgacact aagctgtaat ctgattcgat ttgtctccaa aagttcatat tctaactctt
    12901 gctgtagatc aggcgtctct gcttcttcgt ttggataata cgaaatctca acttttagtg
    12961 tacccgcttc ttttcctgga actacccgat cattttctat gtcaggttct tctcgataac
    13021 taatttctaa aataaagtaa cctggaagtt ttctatagct gtcaggtgac ttcacttcct
    13081 tgatccgata gaccccttcc aacaagtctg aagcattgaa tagggaagaa tcgtttggat
    13141 ctggtgccaa gcctccctcg atgatttcat actctcctgc actattttgt ttctcaattg
    13201 aaaattctgc gccttcaaga cgtcggctat tcatactgtc aattttcaaa agttccaaat
    13261 cgattggcgc tagttcatct tctaaggtta cttgtagtac accatcgatg atttcatagc
    13321 cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc ttcaatgata aacttacctt
    13381 cttcattcag tgcaaactgg aacacttgat cagatagccg atacccattt ggtgcgacag
    13441 attccgagac ctcatattca ccaatagaca gatgcagagg ttggtcgtaa tttaaatcga
    13501 catacttttc tactcctacg gcacgagaat agaagtatcc gatcccaggc aaacgtttcg
    13561 attcttctgg ttctaacgga ttaccagtgt atgctcgata cagcgggaag gaagaatccc
    13621 ccactttcct gatatcgaaa cctgcaccat ctaatgtatg gccttcatgg tctaattttc
    13681 taactcggat ctcccgataa tcaaacgtat tgtaagcagt gaatgctacc cataaacgat
    13741 cctcttcatc tgttgtatac gtgagatatt gccaattttc ttcacctgtt tgcttgagtt
    13801 ggatagtccc tatagcatta tcgtaaagtc gcaagatcca tgtgtcattt tcatcaggtt
    13861 ctttgaaacc ttctgggttc tggctctcaa tgattttgta ttctgcatag ttggtagttt
    13921 cttcactatc cttataaaca aatgaatctg gctctccgat tggatgaata actttctcac
    13981 catccacgat tacatatttc ccgtcttcat ctactgaata gaacgtgagt ctccctttct
    14041 cttcctctac ttcgttcaca ccagaaagcc ctttcgctac tggatatccg atttccagtc
    14101 cgttaccatc tgtttcataa agttcaaact ctgcgccatt gattggcata ttactgcctt
    14161 caattagttt attgatattc atgtggattg gcttgaattc atttcgcatt tccagtttga
    14221 catacaactc atcatactct gtgccttcta tgttaggctc ttgtacaaca gaaagcttgt
    14281 gatattctcc ttcataatag gaattcggat ctttaccttc cggtccgccg actctcgaac
    14341 cagatgtata ttcatctaca agctcgtagt gacctaacat ctcatcttcg ttggctgcat
    14401 attttttgat ttggaattga tacgtctcat ctactaactg ataacgtggt ggtccagcga
    14461 tctctctgat ccaaaatatg tcgctgactg ctccagtttc atctcctaaa atcggcatct
    14521 tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact tgtttcgatt ggttcggctt
    14581 ccccactctc tggattttgg tcatataatg caaattcgac tccagccaac ggctcgccat
    14641 tttgaccctt cttgctgact tggaaattac ggcctttatg ttcgtaacga atggaagaag
    14701 atgggaaata catccgtcca gtagtgatat cattctcata atcctccagg ttctgaatat
    14761 aggccatatt gttcactgtg caaaaatttc cgtcttgagc tcccgcatca agttttactt
    14821 ggtacttgat cctgaccatt tccccttcac ctaaagaaat cggagaaaat ttataagctt
    14881 ggttttcccg tccgacatac gttgtcggag tcgccactac ttctggtggg gcttgtccat
    14941 gacgttcttc atcccatatt tctggctctt ggtacttcat gccgttttct cctggatcta
    15001 gatggtaaaa ctgaatatct gccgatctgc cagctccaaa tggtgagaca ttaaaaccag
    15061 tttcatcata aagtgtggct tgctggatcg ttctcttgaa ggtagaagag atatcctcca
    15121 aggctgtaac aatgtcattc ttgttgttcg cattgtaata tcgcggtgtt acgccatctt
    15181 cttctaaatc agcaatattt ttcaatacat ttcgtccaat agcatctagt cgttgtctcc
    15241 cgctaacgtt tccatcgatt cctaaaccaa tggagaagat acttggcggg ttctcttgat
    15301 acttgttttt cagccaatga tgataagcaa ctgtgttgat cgcagatgac ttttcttgcg
    15361 aagtaggact gccattcgta ttgttattag aagaaccatc accaacccag tgagtcgctt
    15421 tattatctcc ttcttttacc tctccataac gatagtgtac atttacttca tcttctggac
    15481 ggttaaagtc agatgcatag gtaactggat gagtaaaccc tgcattagaa gtattcccag
    15541 aaaactcctc aaaatttctg aacaaaacgt cattatcttc agcaatctta tcttccatca
    15601 cagtccagtt gctccaagct ccattagtgc tcgtacgata acttgactga atcggtgcat
    15661 aactgaaggt cggtgtccca tctcccacaa cgattaggat tttttctgga tttctatttt
    15721 ctccgccatt gtctttatac aatgtttcat agccattttt aagccctagt gtaagcggag
    15781 ttcctccgat tggtgtacga tttaatgctg tattctcact tcccgtcaaa cgattgatat
    15841 catttgtcaa tggaaagatg tccgaactga tttgctcatg gttattgata gctgtactat
    15901 tatggtaaaa attcaccata ccgattcgaa tatccagact cgggtcagag agcagatctt
    15961 tactcatttc gtttactgcc tcaatcaacg cagcattttt agtttgacta ttggtcccag
    16021 cggttaattc actcatactc gctgacttat ctagaacaaa aacgatatcg attggactga
    16081 tttccttttt ctcgcccccg atgacatcta gatagacatt gtaaagggtt gttgcatctt
    16141 caggatgtag atcagatgct gtgctgcttg gcagaacagg ttctacccat tttttgatgt
    16201 acgccccgtt ataatccaag taactgtttt caaaattccg ttcatttccc caaagctgaa
    16261 cggtttgagc tggaacgttt ctatcttctt cgcctgcagt tgatccatta ttagtagcat
    16321 agtcgtaatt tcgccagttc gctcccccgt ttgtataatc actatcatcg atcaagttct
    16381 ctccgtcata tggcgagcct ttgtctcctt gatagcgttc attgctttgt gtccaatttc
    16441 ctaacacttt tgtatagtca ttcgttgtaa acttcatcta ttctttcatc aaccaccact
    16501 tgaagaggta gttacccttc gttcacatcg gggatctttt ctgtttcaaa aatcaatgtg
    16561 ccggtttctg cttcttttga ataaactttt ccgacatacc attcttgacc atcgatcgtc
    16621 tcgactttta ggtcttctcc atcttcatca taggaggata gacctgttcc acgaacgttt
    16681 tttacggtac caatcccggc ggctgccgca tcgattgcta aacgtattct accttcattg
    16741 gcactttcct ttttttcaaa agccagatgc cattcaaggg tttcttcttt ttcaatatac
    16801 ttcccttcaa tttttgcata gtcctcatct aagaatacct catcctcctt tgtttctgtt
    16861 gactcagcag ctacatccag ggtcgtcata tgatagactg actggaaata tggtccaaat
    16921 agcaaactga cagttactaa ctgcaaaagc ttcaatttta accctttcac atccttatac
    16981 tcctttcaaa aatatttgtc ttctttcaca acaatcaaca aaacaatgcc catctaatta
    17041 taatttaatt taatttaatt ttaaatcaaa tgatatcctt tttagttaaa aaacaccaat
    17101 tatacaacat ttattattaa aattaaatta taaaatttta taacgttctc atttaacaaa
    17161 acttaagtta ttaagacaaa tgaataaaca actacgcttt cctcgaaaca aaaaaataaa
    17221 gcacaaatgc gcatttacgc actcatgctt tattcgtaca attaattcag ataacggcag
    17281 cgcataggat gccaataaca cttcctaaaa tagctcctcc taaaatgtca gtaggataat
    17341 gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc aaataaaaag gctaatattc
    17401 ctgcaactgg acttaattcg aacaacgcag ctactgataa aaaggctagc gctgtatgtc
    17461 ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag gacaagccga cgattttttt
    17521 cataaggtct tttccgtctt gtcgtttttt tgataaaaag caaaccaaca aaaaaataga
    17581 tcaaactgcc gataagaatg gctagaccat attctctagt atcaggtatc aagactaaaa
    17641 cagcagcaag gagcaaccaa ataaaaccac cattagagat attactgata aatttcaaaa
    17701 aattgatttt tcgactatcc gttttccgtt gcttggcgaa cttgttgata atactatcat
    17761 cgattttagc aatcaaacgc aacatgttat tcctcctttt taagaacctt ttcttgcgta
    17821 cgatggataa aaagcaggac ttgaaaataa aacaagaaac tgaaacctaa taaagcaaaa
    17881 tagattggtc ctgcaaacca gaatgcgaca agaaggatca acgtgcctgc caaatagaaa
    17941 agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag taatccaaga agcttctttt
    18001 tgttccagtt gaagcaagat tttgtaacta aagagaagcg cgcttaatag aagcaaaaac
    18061 aacgtagtat acatcaatgt atagccaatt cccccacccc aatagcggaa aatctgataa
    18121 tcgatcaata gaagaagaaa ataaacactt gctaaactat cttgctttcc ataaagagag
    18181 aagttctcac gaaacttttt ccagaatagt gaaccgagct ttttctcttc attttttttg
    18241 taatgataaa tcatatacag aactgttcga cttgcagttc ctaatgtgaa aacaggcaag
    18301 acgcacagga accatagtag ctgtaaatgg ataagacgtg gaatccattc aatgatttct
    18361 aagaaataat cattggcttt tttaccaatc gatcgattca tcatcctttt gaacctcctg
    18421 tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga
    18481 tcaagacaag tgttgctcct gccatcagat aattccattg tgtcgttgct tggttctgaa
    18541 aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc ttggatatat aacagaggac
    18601 ctaagaaatc attccaagct gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt
    18661 tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg attcgctccg tcgatttcag
    18721 cagcttcgat caattcatta ttgatcgata agtaaaattg acgcatcaga aatatattga
    18781 acgcattgcc gaagaacgat ggaacgatca ggggcaaata agtacctacc cagccgatct
    18841 tggagaataa tacatattga gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa
    18901 gaaccaaagc aaacatcagc tttttcccag gaaaatcaag cttcgcaaat ccataagcaa
    18961 taaaagaatt tgaaaggaca ttaccgatca ctactagcac agtgatgaat aatgtatttc
    19021 ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag atagttttcc catacgattt
    19081 tttctgggaa aaaggtcgga ggataacgaa cgatttcttc cattggtttc aatgaagtaa
    19141 acaccatcca aagcaatgga atgatcaaaa tgattcctat tactgtcatg aggacataac
    19201 ttagtatttt ttttgtttgc cgattcacac tggtcttttt gaaataatcc atcatttgcc
    19261 gtcacctcca gaataataga cccacttagg tgtaagcttc aaattgacaa acgtcagaac
    19321 caacacgatc agaaacagga tcattgacat agccatcgca taacccatat caaaagctac
    19381 gaacgccttg ttccacatat gcagattata aaaaagcaaa gaatttgcag gtccgcctgt
    19441 tccatttttc gtcatgacat aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt
    19501 gatcacatcg taaaaaataa taggagtgat taatggcaaa gtaatacttt tgaactttcc
    19561 ccaaacgcct gctccatcga tttctgctga ttcatataaa gatctaggaa cattttgtaa
    19621 tgtagctaag taaagtagca ttgctcctcc agaactccat attttcatca aaatcaacgc
    19681 aggtttcgtc cattgtggat caaacagcca agctggtcca gggatatgga accagcccag
    19741 aaatgtgttg atcaatcctg cagatggaga aagcagctgc atccataaga tgtagactgc
    19801 tactcctgat aagactgccg gtaaatagaa agctgtacgg aatacactga ttcctttgat
    19861 tttttgattg agtactgtgg ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa
    19921 gatgacgaaa taaacggtgt tgtataacga tttccaaaat aaatcatcgt gaacgaacat
    19981 tcttttgaaa tttgctagac caatgaaatt catcttggat gtcaaattat aatcggtgaa
    20041 actaccaata actgaaaaaa gcataggccc tagcataaat accaagaacc cgataataaa
    20101 aggcgagata aataaatagg cccatcttgc ttcttttttc tttcggatag ataaacgctt
    20161 caaattttct tgcctcctct gtacctagac agtcaaaaat ggattattga ttttgcttga
    20221 ctaagttttc tactgctttt tgtgcatctg ccaatccttc ttttggtgat tttgttccta
    20281 aaagaatctg gtcaatctgt tcattcacta atgtcgtgta atccggagca tctaaaggaa
    20341 ctggtgtgat caccgtattt ttaaagtttt cgtccgccat ttgatagatc atttttcctt
    20401 tatccgttag gttgtcatta ttcactaaat tctcattggc tgtcttgctc gccatgatat
    20461 caaaactttt ttcgccgaat ttttcttgta cttctggaga agaaagatat ttgatgaatt
    20521 cataagaggc ctcaggatct tttgctccgt atggtacttc caagacgaat ccgccacccc
    20581 acgaccaatg gcctgaacct tcttcttttt ctgggagctg catcacgcca aaatcaaaat
    20641 catccggtgc attttccatc aaactagagt aataattgat attttgggca cgcatggcta
    20701 ctaatccgga aataaatgga tcagatatac cagacccaaa ttcggcttct aatttattga
    20761 tcgtattttt cccataataa tcttgccagt cttggatcca ctccaaggct tccactttat
    20821 ttttagtatc aatcttgact ttttcatctt tgtcaaacca actagtcccg ttatctgcgt
    20881 ttagcgccca tacatcagcc cctaaattcc ataatggata aaaaccgatg cgtgtaaatt
    20941 cttttccttc ttttttatct agtttacgtg ctgtttcttc taattcatcc caagttgtcg
    21001 gtagatcttg ttcactgatt ccagcttctt taaataactt tttattataa aaaatgactt
    21061 gtgtatctgt attgaatggg atcgcataag gatcaccttc atattctact gtatcccaaa
    21121 gctgcggata aaaatcctta gaagtgtctt tttctacata tttggagaga ttagttgctt
    21181 gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg tacaataaca tcgggtgcat
    21241 ttccagctgt tattgcagat aaagatttag tccagatgtc accccatggc tgatacttat
    21301 aatttacatg gatctcgttt tgtgattgat tgaaatcctc aatgatttct tcgatagctt
    21361 cttttcgtgc accggatccc caaaaggacc agaaatcaat attttttctt ccatcacttg
    21421 ttttttcaga agaatttgct gcacttgaac tggcatcgct tgcattactt gatccgcatc
    21481 ctcctaataa aaacaatgct gaacttgcca gcaaccctgt aaacaataat tttttcatat
    21541 tttacctcca aattttaatg taaccgat
//
LOCUS       DAXWLB010000034        21240 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXWLB010000034 DAXWLB010000000
VERSION     DAXWLB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.034"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..3668)
                     /gene="nifJ"
                     /locus_tag="ACGE4H_000984"
     CDS             complement(<1..3668)
                     /gene="nifJ"
                     /locus_tag="ACGE4H_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFF8364419.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKE"
     gene            3866..4387
                     /locus_tag="ACGE4H_000985"
     CDS             3866..4387
                     /locus_tag="ACGE4H_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFF8364420.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(5086..5651)
                     /locus_tag="ACGE4H_000986"
                     /pseudo
     CDS             complement(5086..5651)
                     /locus_tag="ACGE4H_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5830..6486)
                     /locus_tag="ACGE4H_000987"
     CDS             complement(5830..6486)
                     /locus_tag="ACGE4H_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364421.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(7054..7989)
                     /gene="whiA"
                     /locus_tag="ACGE4H_000988"
     CDS             complement(7054..7989)
                     /gene="whiA"
                     /locus_tag="ACGE4H_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFF8364422.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(8023..9021)
                     /gene="yvcK"
                     /locus_tag="ACGE4H_000989"
     CDS             complement(8023..9021)
                     /gene="yvcK"
                     /locus_tag="ACGE4H_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFF8364423.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(9018..9902)
                     /gene="rapZ"
                     /locus_tag="ACGE4H_000990"
     CDS             complement(9018..9902)
                     /gene="rapZ"
                     /locus_tag="ACGE4H_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFF8364424.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(10037..10768)
                     /locus_tag="ACGE4H_000991"
     CDS             complement(10037..10768)
                     /locus_tag="ACGE4H_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFF8364425.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(10772..12037)
                     /locus_tag="ACGE4H_000992"
     CDS             complement(10772..12037)
                     /locus_tag="ACGE4H_000992"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFF8364426.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(12300..15116)
                     /gene="uvrA"
                     /locus_tag="ACGE4H_000993"
     CDS             complement(12300..15116)
                     /gene="uvrA"
                     /locus_tag="ACGE4H_000993"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFF8364427.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(15126..17120)
                     /gene="uvrB"
                     /locus_tag="ACGE4H_000994"
     CDS             complement(15126..17120)
                     /gene="uvrB"
                     /locus_tag="ACGE4H_000994"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFF8364428.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            17379..18881
                     /locus_tag="ACGE4H_000995"
     CDS             17379..18881
                     /locus_tag="ACGE4H_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFF8364429.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            18883..19620
                     /locus_tag="ACGE4H_000996"
     CDS             18883..19620
                     /locus_tag="ACGE4H_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8364430.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(19988..21154)
                     /gene="dnaJ"
                     /locus_tag="ACGE4H_000997"
     CDS             complement(19988..21154)
                     /gene="dnaJ"
                     /locus_tag="ACGE4H_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFF8364431.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         6045 a         4669 c         3650 g         6876 t
ORIGIN      
        1 ttttcttttg tagcttgttc cattttttta cgtcgttctg ctctggcagc tcttctttct
       61 tctgcttcaa ggtccgtcgt tttttctttt ctagtcttaa cgggtacatc gatagattcc
      121 tctggttcaa gtcgtttctt gattttttct aattctcccg tcagtgcagc atagttgtaa
      181 aaacgtgttt tagcgtcttg gatagtcttt tcaaataaag cttttgcaag atttggattt
      241 gcgttctcta gcgaggaaaa gcgaacttgt ttcctcatga aatcaggcat agcagagaaa
      301 tcaggttttt tgtagtccaa agtcattggg ttcttgtttt tttcgaccat tgcagggtta
      361 tatcgatata atgaccaata gccagaagaa acagcttctt ttgcttcttt aagcgattct
      421 ttcattccac caagcaaacc atgtgtgata caaggcgtat aggcaatgat taaagaaggt
      481 ccaggatact tttctgcttc ttcaaaagcc tttatcgtct gcatttgatt ggcacctgaa
      541 gcaatttgcg ccacataaac atttccatat gtcatcgcca tcattcctag atctttcttt
      601 gaagcatact ttccactagc cgaaaattta gcgattgccg aagctggtgt tgcttttgag
      661 gtttgaccgc cagtattgga atagacttcg ttatccaaaa caagaatatt cacgtctgct
      721 ccgctggcaa gtacatgatc gatcccgcca aaaccaatgt cgtaagccca tccgtctcca
      781 ccaatcatcc attggctgtt ttttacgaat agatcctttc tcttgtatat atcctccagt
      841 tcagggacaa ttgatcgttc gctttcgatc gcagcttgca gttttgctgc tctttgctgc
      901 gttccttcgc tctcaaacat gtgtaccaac cagtcttcca tcaattctcg tagttcttct
      961 gatacttttg gcaatacttc tttgattgaa cttgccaaag cctcgcgacg agtctgtgcc
     1021 gctagaagca taccataacc aaattcagca ttatcttcca ataaagaatt tgaccatgca
     1081 ggcccttgac cacattcatt ggtcgtataa ggagaagcag gagaagcacc gccccaaata
     1141 gaagaacagc ctgtcgcatt cgcgatcatc attcgatcac cgaacatttg tgtcaataat
     1201 tttacataag gtgtttcgcc acagcctgag caagcgcccg agaattcaag aagcggttga
     1261 ttgaattgtg tagacaagac agtattcttt cctttgactg gatttgcttt ttgtcttaat
     1321 gtcattgcaa aagcccaatt cacagcttgt tctttctgtt cttcataagg cttcatcact
     1381 aaagcttttt cttttgcggg acaagcctct acacacagac cacaacctgt acagtcttcc
     1441 actgaaactt ggatacggta ttttacgccg tctgcgcctc gcatttctct cacgataaat
     1501 ccttctggcg cttcttccat ttcctcttca tctgctaaaa atggacggat ggcagcatga
     1561 ggacaaacaa aggcacattc attacacatc gtacagcgat cactaatcca ctcaggtact
     1621 tctaaagcga cgccccgctt ttccatagaa gccgttccta aaggcatttc gccagttgtc
     1681 attccatttg ctacaagtgt accaacagag agttgatttc cttcctgtgc attcactggt
     1741 tcaagtattt cttgtatata accagtgttc cttttattga ttttttcagg catctccaac
     1801 gttctccaat ggtctgggat cgcgacttga tgaagcagtt cggtcgtccg atcgattgct
     1861 tgtatgtttt tttctacgat agccattgat ttatgtccat agcttttcag ggcttcttct
     1921 tttagcagcg gaagcacctc atcaaaagga atgatgtcag ctaatttaaa aaaagccgtt
     1981 tccattgccg tattgatccg gcgtcccaaa ccgacttctt gggccaatct catggcattg
     2041 atcgtataga atctaatgtt attttcagcc aagtaacgtt ttaattttaa gggcagatgc
     2101 gtttctaatt gttcgtcgct ccaaagagta ttcaataaaa aaatgccacc tggcttcaat
     2161 cctttgacca agtcatagct atgcaggtaa gctggtgtgt gacaggcaac aaggtccgca
     2221 tgctcgacaa gatacgctga acgtatcggc gtatctccaa aacgaagatg agagacagtc
     2281 agaccgccag attttttcga atcataataa aaatagcctt gcgcatattt atcggtgtga
     2341 tctccaataa tcttgatagc tgatttattt gctccaactg ttccgtcaga tccaaatccc
     2401 caaaacttgg cctggaatgt ttgcggttct gtcaaatcca gactttcctt tggttccaaa
     2461 gattgataag tcacatcatc cacgatccct atggtaaacc gttttttgcg tatgcttggg
     2521 tcttttttca attcatcaaa aactgctgag atttggtcgg gagtcacatc tttagacccc
     2581 aatccgtagc gaccaccaat aacagccggg cgaagttcag aatcatataa cgcactttga
     2641 acgtctaata agagaggttc tccacctgcc cctggttctt tacttcgatc aagaacagca
     2701 attgttttta ctgtatgagg caacttttgc acgaatgttt ccactggaaa aggacgataa
     2761 agatggatat tcaaaaaacc tgttttccgt ccgtttgcat tcaagtaatc aatcgtttgt
     2821 tcaatcgtct gtgcaactga tcccatagaa acaatgactt cttccgcatc gtctgctccg
     2881 taataagtca ccaagtcata agaagttcct cgtaatcggt tgatctcttt catatatttt
     2941 tgaacgattt caggtaattt atcataatag gggttgatcg tttcccgttg ttgaaaatgg
     3001 atgtctggat tctggttcgt accactaaca gtcggatgat tgggattcat ccctcgttta
     3061 cgaaatgaag ctaatttctc ctgattgatc agaggagcta attcttcata ttccaaaact
     3121 tcgatttttt gaatctcgtg actcgtacgg aatccatcaa agaaattcaa aaaaggtact
     3181 tctccttcaa tagaagcaag atgggcaaca ggtgctaaat ccatcacttc ttgtacactg
     3241 ctttcagcta acatagcaaa tcctgtttgt cttgcagcca tcacgtctcc gtgatctccg
     3301 aaaatattca acgcatttgt cgtcactgcc cgactagcaa catggaaaac acttggcagt
     3361 agttccccag caatcttgta catattcggt atcatcaaaa gcagcccttg agaagcggta
     3421 tatgtagttg tcagtgcacc agttttcaat gatccatgga ccacaccagc tgctcctgct
     3481 tcggattgca tttctactac ttttactggt tgaccgaata cattttcttt tcctgctgct
     3541 gaccattgat ctactaattc agccatggtc gaactgggtg ttattggata tattgcagca
     3601 atctcggtaa atgcataaga aatatatgcg gctgccgtat tgccatccat tgttttcatt
     3661 tttcgcatga ttcatcatcc ttgttcatat tctctcttat tatctgttcc attataacgt
     3721 aaaacattcc attttttctt ttatttataa aaatatttca catatgtcta tttgaattgg
     3781 tcatctaatt tttagacaaa cagtctaaat cgttgccttt ttttgtcaga catgtataaa
     3841 ctataagcga aagaggtgtt ttaacatgga tatatcagtg attgatgcaa cgaaaacaaa
     3901 tacacaaaaa ggaatccttt atggttcatc gaatgctcct aaaaaaatgg tcgaattcat
     3961 caacctatct tgtccgtatt gccgacaatg gtttgaagaa tcttatgagc tattagaaga
     4021 agcagtccaa agcgggcaat tgcagcgagt gatcaaattg ttcgataaag aaaaagaaag
     4081 cttgcagcga ggaaacgtga tgcaccggta tctcacaatc agcgatggtc aaaaagcgat
     4141 caaagagatc aaacaaatat tcgatactca agacgagtgg aaacatcttt ctctccaaga
     4201 agtagcggat tttgcagtag ataaactaaa gctgacagaa caaaaagacg aacagctgag
     4261 ccaagctgtc atcaacgaag ctgagcaagc acatattcga tttgttccga ctgtcatctt
     4321 gggtaaagaa atctttgatg aatctatttc aatagaggaa ttaaaagaat tgatccaaac
     4381 aaaataaaaa aataaggagc gtaggacaaa agtaaagaac acttttgtct cacgctctaa
     4441 acacctataa acggcggaag cagaagcaac tccttcttat ttcttggagt taaacgcttc
     4501 tgtcccagcc tccttattcc gaataaatgg tgtccaaaag cttgcatctg ccagattatc
     4561 tcatttctta tgacaagctg agcaaattat ctccaccaaa taatttattc atatttttga
     4621 cttgcgcata ctaactaatt cttaaatttt catacaaaat ttctcaaatt cccttaacga
     4681 gtaatattta tgtacggtga ttcacactag tatcccaatc actttttaag ttatcccgat
     4741 ttcaattcta cttcaaaaat agttgctttt atgtatgctg ttagttgatt agatggtcgt
     4801 ttgttgagct aggtcgttta ttttaaacaa gttgtttctt tagcttatac ctatctcgtc
     4861 taccaaacgc aagatggcaa acacaaatgc atacgcatct gtatttgcca ctttgattcg
     4921 tataaagcta acaactccta actcctctca tttaatagtt gctctctttt ttattcagat
     4981 acatttttgc tgatcacagc ctagtatttc aatggttttg aacactaaac caaattgttc
     5041 cattacaaca ttcactggct gatgaaatgg attaatagat ttcgattatt caaagcgatt
     5101 ttctattggt ggattgtgtt cccaactaac aagacggatt ccaaagtcat tatataactc
     5161 ttcaagagtt aaccctttcc tgttcccaat agatgtatct ctcccacgct cgatataaaa
     5221 tatggagttt cgaatgccaa gacctggcaa atctttcctt gttacagtta cactgctact
     5281 aacttcccca tataaaatcc agctcctcca aaatgataat atatttttcg accatcatct
     5341 aactcatgta acaataattg ttgtgccctc ttgtcactcg ccaaatgcaa acgccacgtg
     5401 cgataccatg ccatgctacc tgcatctgca gaaaatccat caaataatgc aacaattgta
     5461 tcttctatca caatcctttc cccatctact tcatattcta atctaaaatc aaactcccct
     5521 cgggtaatcg caggtcttgg tggcttagga cccaatatta ttctacttaa aaccgcattc
     5581 gttcccagat gaaacacagc tataacaaca ataatgatta atattttgtt tctatttgat
     5641 aaatttctca ttattcgcaa atcacctcta cattctcaca tactagttcg taatactcct
     5701 ttaatatcaa aatatctact gatcgtaata atattcgcta tgaattccta ctactcgtat
     5761 aagaaaggcc aaatcatcag cttttacttg ctgatgattt ggcttgtttc tatttatcgt
     5821 aattgttttc taatcaccaa aattgttaac aatcggttca gatatttccc aattcactag
     5881 ccttatatta tattgattta acaattcttc ttcatttatt gtattttgat caagcggacc
     5941 aaattgttct aataattctt tgtcatgtaa aatatccctt ctagtaatgc ctctttccct
     6001 ttcaatgaaa gaagcactag gaaaagtatg attataacca ttcaacctat ctcccatgta
     6061 ataattagca ggtcctggaa aataaaatat ttgcttatta tcactaactt ctaacagcaa
     6121 cactaaatct tctccgctgg ctaaacgact tgtccatcta cgatatctcc ctatgccttc
     6181 gttcattcca aatccatcaa attcaactat taacgtgtct tctatcacat gtaattcttc
     6241 attcaattca tactcaagtc taaaaggaaa ttctccatat gtaatctctg gcaatggtgg
     6301 tgctggagtt ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat
     6361 aaatgttatg agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt
     6421 tgctatatct agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa
     6481 acgcattttt gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttggta
     6541 gttcaagttc aggtataacg tattccaacc atacatctca attactattt atgaacttat
     6601 gattatcact gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg
     6661 gttgttttcg actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag
     6721 aatcttgacc agaagtaaga agagcagttc tcaaggatga tcgagcagtt ccttccagtt
     6781 ttttaattaa tcaggccagt tatagataaa tacttgatct tatctatatc aggacttcta
     6841 tattaagcaa aaatttatgt acaaaactaa aatcttcatt cagttgtctc cccgcttttt
     6901 atacgattgt attaaaaaca atagaagcct ttccaaatta tgttactcca gtaaagatgg
     6961 aattatagtt tgacggaaga aaagcaagta taacttttaa aaaaaactga tacattcgca
     7021 tctcctgctt catgtatcag ttttttcttt ggattaaact gcttcttcac gcagtttttc
     7081 cgcaaattca tttattttgc gaattcggtg attaattccc gattttgata tcgcacctga
     7141 gggaatcatt tcccctaatt ctttcaagct gacctctgga tgttgaagcc gaagttctgc
     7201 gatttcctga agtttttcag gaagggcatg tagaccgacc tttgcttcga tcaattcgat
     7261 gttttcgatt tgtttagaag ccgcatcaat cgtcttgttc agatttgctg tctcacaatt
     7321 gactagacga ttgaccgaat ttctcatatc ccgtacgatt cgaacatctt caaatcggag
     7381 cattgagttg gttgcaccaa tcaatgttaa gaaatcagca attttttctg ctccttttaa
     7441 ataagaaata tagccattgc gccgttcaag cgttcgagca tgtagaccgt aataatttaa
     7501 catatcgcaa atgtcttggt tatgctgttc ataaatagaa aatatttcca aatgataacg
     7561 gctcgtttcc ggattattga ctgaacctga ggccataaaa gcaccacgta agtaagaacg
     7621 cattttttgc gaatttccca taatctcgtc cgaaacgtgt ccattgaaca tcataccatc
     7681 catgatatca agatccatca acacatcttt tgtgccttgc ttcaaccgga cgatatagac
     7741 attgttcttt tttaatttca tttttttacg aacgagtagc tcacttcgta cctgataatg
     7801 atctttcaaa agagaataga tacgtctggc aattgcagca ttttctgttt ggacgttcaa
     7861 aataaactgc tggttcacaa tacctaaaga gccgttcatt cgcagcaaag cagctaattc
     7921 cgctttcgca tgttctcgat gaacttccaa accagttagt tctttcttca catctgcagc
     7981 aaatgacatc ctattctcct cctttctcca acaataaatt gcttaatatt ttgaaccgaa
     8041 aactaaacgg aacaattctt ctactacttt gtctccatcg tggaagacac caccatcttt
     8101 caattccaaa aagtcggtgg aaatcactcg acatccttca tttctcaatc cgttgaagtc
     8161 gtgtgcgact tgtactagat actcatcata gatctctggg tccatataat cttcaggcac
     8221 tttttccgta ttgaccaata ctgtatcgat aaaaggctga ttcaaatgct tgtgtaaaac
     8281 acgtatatga tcggcatctg tgaaatgttc cgtttcccct ttttgggtca tgatattaca
     8341 gatatagacg gtttcagcag ttgtcttgag cattgcttcg ccgatttctg gtatcactaa
     8401 atttggaagg atactggtga ataaacttcc gggtcccaaa acgaccatgt cggcgtccat
     8461 aattgctgaa acgacttttc gagccgcttt ggcttcactt ttatcatttg tgttgcagac
     8521 atacacacgg tcaatggttt tacggtcgat cgcgatttta gattctccta ctgcagtcgt
     8581 tccgtcttta aagacagcat gcaaggtcaa aggagtttct gaagaaggat aaacatgtcc
     8641 atctacatgc atcatttttg ctaataattg aatcgcctca taagtgcttc cccgcatctc
     8701 ggaaactgcg gcaatgatca gattaccgat cgtatgattg gctaaaaact gatcttcctt
     8761 tttgaaacgg tattggaaaa tatcttcata gaattgtggc atatccgata acgccactaa
     8821 gacatttctc aagtcaccag gaggagccat agcgatcgat tctctgattg caccgctgct
     8881 tcccccatca tctgcaacag tcactactgc tgtgatatct gctccttgat ttcgcaaact
     8941 tttcaggata actggcagac cagtgccacc gccgaccact acgatctttg gttttcttat
     9001 ccggtaggtt ttcattttca cgaccggttc accgtctctt tccttttatc cttgtcccga
     9061 tgtgtcacat tgactttata gtttttccct aaagcatttg ccaaacgttc agtcaatgcc
     9121 acagaacggt gttgaccgcc tgtacagccg attgcaactg tcaagctgct ttttccttct
     9181 ttaatgtatc caggcaatac agtttccaga agatccaaaa atttatggta gaatgattgc
     9241 gtttcatcaa aggacatcac ataatcatag actggctgat cttttccggt caatggtctt
     9301 agttccggga tataatgagg gttgggcaaa aaacgcacat ccatcacaat atccgaatcg
     9361 atcggcaacc cgtatttaaa accaaatgat acaaattcga tccgaaatcc ttggtctccc
     9421 tttgatttga atgactcatt gattttttca cgtaattgtc gtggtgtcaa tgtcgtcgta
     9481 tcaatgatca cggaagcttg ggctttcaag tcgtctagta tcgcccgttc tttacgtatc
     9541 ccttcagtga tcaaaccgtc catcgccatt ggatgtgctc gtcttgtttc tttgtagcga
     9601 gccactagtt cttcgtctga agcatctaaa aataagatag aagtgtcgat gaagttcgta
     9661 ttttcgattt cgatcagcat gttttggatt tcttcaaaga aggaacgaga acgtaaatcc
     9721 actcctaaag ccatttttgt gactttgcct gattctttga ttaattccca gaattttgga
     9781 atcaatcgag gcggcatatt atcgatacaa aaatagccca tatcttcaaa actttggata
     9841 gctactgttt tccctgcacc gctcatacct gttatgatga ctaattgcaa attatctgtc
     9901 ataattacga actcctctca cattgtgcat agtaaaatta tatcattccg ggcgtatcat
     9961 taccagtttt ctttttcttt tacaaaaaga tttggaccaa atatctttta ggatatttgc
    10021 cccaaatcca atttttttac ttttccgatg ctgtatccaa tgccttgctt ctttcagcta
    10081 gcgctcgttc aatcgtccga tctgctaaaa tagattgggc atcgatcact ggataatggt
    10141 tatcttcagc aaattcatgc atcaatgaca attctgtgca acctaagata actttttcac
    10201 agtttaggcg ctctaccgct tcttctaaga tttcgtaata tagctcttga ttcaaataat
    10261 ccgattcctt gatctcatga tagatcagat aattgatttt ttcttgaaga acagtatcag
    10321 gaatcactgt ttcgaatcca agatttttga cttcgcgttc atagattcca gctttcatac
    10381 ttccttctgt tcctagaatc gctactctcc ctgtcgtatg ctgacggaca agttcatttg
    10441 ctgcttctct tggcatatga aggataggaa tatccgttgc cgcttgtaat tcttcaaaaa
    10501 agtagtgtgc ggtgttacaa gtcaacacaa tgaagttcgg ctgcaataaa ttttgtttct
    10561 caatatcatc cagcagaaac ggcattggat tttcttctga tcgatctaaa atataagctg
    10621 tacgatcagg aactgttgca tgattgaata agacataatt caaatattct tgatcttttg
    10681 tagctttcgt tcggtggttg atcaagcgaa caaaactttc tgtcgccatc gtgcccattc
    10741 cgcctaaaat actgaaaaaa ttctccatga actagccttc tttcacatga aaatactttt
    10801 tgaatcttgg aatatagtta tggaacatat atttcatcaa caaccagcgc ttgattgacc
    10861 ggtctttttc ataaaacaca gtagtaccgt aacgattttc tttgatcatt tgctcagcta
    10921 gttttttgtc ttcattttcc cgagcatatt ctaaaaagat ttttttgggc acacccatcc
    10981 ataatcttgc tttatctgat tgattcgttc cataggtcgt ttcaacaaag ggtttgttaa
    11041 acactcgatc ttctgtgacg aaacgagcca gattcaagcc gttcaatgtc acaaagaaac
    11101 tgctacgacc ttgtctcaaa ttgatttcaa ataatttata ttctccatct ctaggatcat
    11161 acttcatatc gaaattggca aagcctgtat attcgatttt ttccaaaaat gccttaatgg
    11221 tttgatagat tttttcgtta taatctggca tgatcacaac gtaattacca atcgatgctg
    11281 gtgtaggatc ttctaacagt ggatgaccaa gacacatcat ccgaacttga tgatcttcat
    11341 ctacatacgc atttagcaca cgcatattac tatcatcacc cgggataaaa tcttgtacaa
    11401 tcatttcact tttataaccg gcttcgtata tccggcctaa aattaaatcg aactcttctc
    11461 tagtctctaa aataaaagct ttttttcttc cttcaaattg gacagaaaga tactccacac
    11521 tgtttgcagg tttcaaagca actgggaatt caaaaggcaa ttcctgttcc aaatgtccat
    11581 ttactaacat ttcttctctg acgatcaatg ttttaggata cggaagatca tattcttcac
    11641 aaacttcata aaagcttact ttattgatca gtctttcaaa taaagaataa tcaatatatg
    11701 gacagataaa atattctgac agctcttgtt tatgttggga gatcaattct gcatatccat
    11761 caccacaagc aatcaaaaga tattttttac tcttatcaga atatctttct tttgccaaac
    11821 gaagcatcgt ctcgataaac acagggtctt tatcaaatcc tggaataact tctacattga
    11881 caattttact gtagcgggta ggggctaact gatccgaagc atatgcttgg cagattttgc
    11941 cgtatgcttc attaaaagaa cgtgccatac cataaacgtt catgtcgctt cctaataata
    12001 tagggataaa ttcttcattt tcaatactgt tcatcattat ctctcactta ctcataatct
    12061 cttgtttttc ttttccaact acacaaggaa tatcatacca taaagaaaaa agcaatcaaa
    12121 ggaaactcct ccgattgctc aattcttttt tgaacggatt gaacttatag gaagtttttg
    12181 tctataccaa tcagtgtaga aagaactacc atgtaatgat tctagaacac tgatattaat
    12241 aaaacaagca aaaattcatt tttttatgtt tttttaacta tctcagctat ctatcctctt
    12301 tataccatta tttttttcaa atagtgacct gtataacttt cttttacgtt aatgatttct
    12361 tctggtgttc cagtcgcaag gatcgttcct ccaccttctc caccttctgg tcctaaatca
    12421 atgatataat ctgctgtttt gattacatct agattgtgtt caatcactaa aactgtgtta
    12481 ccagcatcta ctaatctttg taatacatgc aacaagcggg cgatgtcatc gctatgaagt
    12541 cctgtcgttg gttcatctag tatatagaaa ttctttccat tagagatttt gtgcaattca
    12601 ctggcaagtt tcatccgttg tgcctcacca ccggacaatg tcgttgctgg ttgccccatc
    12661 gtcacataac ctaagccaac atcaacaatc gtttgcagtt tgcgatgaat ctttggaatg
    12721 tgcttgaaga attctacagc atcttctact gtcatttcca aaatatcagc aatgcttttt
    12781 cctttgtaat gcacttctaa tgtttcagag ttatatcgtt tgccatgaca tacttcacaa
    12841 ggaacataga catcaggcaa aaagtgcatt tcgatcttaa taattccatc cccgcgacaa
    12901 gcttcacaac gaccgccttt tacgttgaaa ctaaaacgcc ctttcttata accccgcatt
    12961 tttgcctcgt tcgtttgagc aaataaccca cggatatcat caaatacact tgtataagtc
    13021 gctggattac ttctcggcgt ccggccgatt gggctttgat cgatatcgat gatcttttcg
    13081 atactttcgt agccggaaat tgtcttgaat ttacctggct tagcagaatt cttatttaat
    13141 ttttgcgcta acgatttctt taagatacta ttgaccaatg tactctttcc agaaccagac
    13201 acacctgtga ctgctacaaa ttctcctaat ggaaattcaa cactaatatt tttcagattg
    13261 ttttctgaag caccagtgat cttgattgct ttcccattac ctttacggcg ttcttttggt
    13321 acagggattg attttttccc tgaaagatat ttccctgtca aagaattttc atcattagct
    13381 acttcttcag gagtgcctgc agccacgatt tgtccgcctt gttctccggc cccaggtccg
    13441 acgtcaatca gataatctgc tgcccacatc gtatcttcat catgttcgac tacaacgagg
    13501 gtgttgccaa gatcacgcat tttctttaat gagccaatca gtcgatcgtt gtcgcgttga
    13561 tgcaatccga tcgatggttc atctaaaata tacaaaactc cagaaagatt cgatccaatt
    13621 tgtgtagcca atctgattcg ctgcgcttct cccccagata atgttccgga agcgcgactc
    13681 aaagttagat agtctagtcc cacattttcc aagaatgaaa gtctgtcatt tacttctttt
    13741 aaaatcggtt tggcaattac tttctcttgt tcagataacg ttaagtttga gaagaaatct
    13801 actgcttttt gaatagacaa ctcatttgtt tgcccgatat tcgttccatt gatttgaaca
    13861 gatagagctt ggggattcaa tcgataacct ttacatgtct gacaagttaa ttctgtcata
    13921 taaagacgca tttgctcacg cgtgaaatca ctgttcgtat catgataacg acgctcgata
    13981 ttcttcaata ccccttcgaa agtcgtttct acatcgcgaa cgccaccaaa atcattttca
    14041 taatggaaat ggaaaagatc atctgtccca ttcaagatga tatcctgatg ctcttccggt
    14101 aattcttcga atggtgtatc taaatcgata ccgaaacttt cacaagcctg cgctaacatt
    14161 tgaggataat actgagagct gattggattc catggaacga tagcaccttg actaagtgtc
    14221 ttcgttctat ctggaatcac taaatcttga tcgacttcta atttgacacc taaaccatca
    14281 cagtctggac aagctccaaa aggcgcattg aatgagaaca gtcttggttc tagttcgcca
    14341 acagtaaatc cgcaataagg acaggcatag tgttcgctaa acaacatttc ttcttcaccg
    14401 atcacgtcaa ccagagcata gccgtctgcc aatcgcagag ccgcctctaa agaatcaaat
    14461 agacgagaac gaatcccttc tttcacaatg atccggtcga tgacgatcgc gatatcatgt
    14521 tttttgtttt tctccagctc aggcacttca cttaaatcat agatctcacc atcgactcga
    14581 acgcggacgt atccctcttt ttggattcgt tcgaatattt ttttatgttg tccttttttc
    14641 tttaccacga tcggtgccaa cacttggatc tttgtgcgct caggcaaagc cattacttga
    14701 tctgccattt gatctactga ttgactggta atctcgatat gatcgttggg acaaatcgga
    14761 tgtcctacac gtgcaaacaa caagcgtaaa tagtcattga tttccgtcac agtacctact
    14821 gtcgaccgag gatttttact tgttgttttc tgatcaatag aaatcgccgg actcaatccg
    14881 tcgatgctat ctacgtccgg cttatccatt tgtcctaaaa actgacgagc ataagcagaa
    14941 aggctctcta cgtatctccg ctgcccttca gcgtacagcg tatcaaatgc tagggaactt
    15001 tttccagaac cggacaaacc tgtaacgacc acgaatttat ctcgtggaat cgttacatct
    15061 atatttttca agttatgggc acgtgcaccg tgaatcgtaa ttttatcgtt agccatgttg
    15121 tcctcctact ttgaagcttt taattctaaa atcatatctc gcacattagc ggctgtttca
    15181 aaatccaatg ctttcgccgc atttttcatt tctttttcta atttcatcaa taacgtatct
    15241 ttttcttctt tcgtcatttc ttcatacgag acttgtaccg cttcttttgt atcatcttcc
    15301 gattctttcg taatagaaat caaatctcgg atttctttaa tgatcgtttt tggaacgatc
    15361 ccatgttctt cattgtactt ctgctgaatc gtacggcgtc ttgatgtttc atccatcgct
    15421 agacgcatag aatctgttac tttgtctgcg tacatgatta ctttaccttc ttcattccga
    15481 gcagctcgtc caatcgtttg gactaaggaa cgttcactac gtaaaaagcc ttctttatcc
    15541 gcatccaaga tagcaacaag ggaaacttca ggtacgtcta atccttcacg aagcaaattg
    15601 attccaacta acacatcaaa ttcacctaat cgaagatctc gaatgatttc tgttcgttcc
    15661 agcgttttga tatcactatg caaatatttg actttgatcc ctaattcttt gaaataatct
    15721 gtgaggtctt ctgccatttt tttcgtcaat gtcgttacaa acacccgctg gtctttttcg
    15781 actcgttcat ttatttcgcc tactagatca tcgatttgtc ccatgattgg acgaacttcg
    15841 atcactgggt caagcagccc tgtcggacgg atgatttgtt ggataacggt atctgtttgt
    15901 tcatgttcat atggtcctgg cgtcgcagag acataaatga tctgattgac atgtttttca
    15961 aactcttcta accgaagtgg ccgattgtct aaagcagatg gcaaacggaa accgtaatct
    16021 actagcatct gttttctcgc tcggtcacca ttatacattc ctctgacttg aggcatggtt
    16081 acgtgcgatt cgtccgccac gatcaaaaaa tcttcaggaa agaaatccaa tagcgtataa
    16141 ggcggttcac cttctttacg tccgtccata tgccgagaat aattttcaat tcccgacgtg
    16201 tagcccattt ctaacatcat ttcaatatcg taattggttc gctgttctaa acgttgagct
    16261 tccagaagtt tgttttcatt tcgcaagaca gttaaacgct gttctaattc cgctttgata
    16321 ttcgctacgg catgctccat atgttcatca ttcgtcacaa agtgagtcgc cgggaagatt
    16381 gccacgtgtt ccgtttctcc taaaacttca cctgtcagtg cattgacttc tcggatacgt
    16441 tcgacctcat ctccaaaaaa ctccacacgt aaggcacgtt catctcttga agccggaaaa
    16501 atctccacga catcaccgcg cactcgaaaa cgaccgcgct gaaaatctat atcatttctt
    16561 tcgaattgga tactgactaa gtcacgaatc aactgattgc gatctagttc tgctccctgt
    16621 cgaatcgaaa ctacttgttt ttggtactca aaaggcgaac ctaatccaaa aatacatgaa
    16681 acggatgcaa tgacgatcac atcatttctt tctaataatg aactagtagc cgaatgtcgt
    16741 aatttgtcga tctcatcatt tacacttgaa tctttttcga tatacgtatc gcttgaaggc
    16801 acataagctt caggctgata ataatcataa taagaaacaa aatattcaac agcattgtta
    16861 gggaaaaatt ccttgaattc cccgtacagc tgaccagcca atgttttgtt atgggcaata
    16921 attagtgttg gtttattcac tttctcaata agattggaaa tcgtatatgt tttccctgtt
    16981 ccagtcgcac ctaataagat ttgtgctttt ttcccgccga ctacgccatc cactaattgg
    17041 ttaatcgctt caggctgatc accagccggt tggtatttcg agacaagctc aaagtgtcgg
    17101 gaggtatctc tctcaatcat tcttcgtcct ccttttcatt cattcctttt cattttacga
    17161 ccaataaaaa gaaaaggaaa gcaaaggcat tccttactaa aaaattatac cacaccgaac
    17221 atactttcgc cactttttta tcttattatg catgttacgt tttataacat aaaatgttag
    17281 aaactcgcat ttttttcatt ttttcgagct tacagtcttg tctgattcag cacttttcgc
    17341 ctataataat aaaatattgg aaatttagga ggttgattat gaaaagaaag catttatttc
    17401 actcatttgt tttcttaatg acaggacttg tgacacttgc gtttggacat gttgcacaag
    17461 ctgacaacac tacaacggca gagtcaagca caagcgagac aatgcaaaaa atagagccca
    17521 aaaaagacgt atacgtaatt gccagtgact ctgcttttgc tccatttgaa tttcaaaacg
    17581 caaagggcga gtacgaaggc atcgatgtaa aattgatgga aaacatcgcc aaactgcaag
    17641 gatttaacat ccgaatggac taccgtggtt tctctgctgc tttgcaagct ttggaatcag
    17701 gacaagcaga cggaatgatt gccggaatga ctgtaacaga tgaacgaaag aaaagctatg
    17761 atttttctga ttcttacttt gatagcggga tccaattagc tgtcaaaaaa ggagatactt
    17821 cgatcaagtc ttatgaagat ttgaaaggaa aaacagtcgg agcaaaagtt ggtaccgaaa
    17881 gtgctgaatt tttaagcgac catgaatctg aatatggcta ttctatcaaa tattttgatg
    17941 ctgctgatca attgtacgac gggttaaaag ttggcgcaat cgatgcaatg atggatgatt
    18001 atccggtaat cggttatgcg atcaaacaag gacaagaatt ggaaacaccg atcaaaaaag
    18061 aatctggcgg aaaatatgca tttgccgtta aaaaaggaca aaatcctgag cttcttaata
    18121 tgttcaatga aggattgaaa gaattaaaaa gaacaggcga atacgataag attattaaag
    18181 aatatgtagc ggatggttca gaaacttcca gtgagtcttc cactgctgat gaatcaacat
    18241 ttcttggact tatccaaaac aactatcaag cattgttgaa aggactttgg agaacaattc
    18301 tcctaacttt gatctccttt gctttggcta tagtaatcgg tgtgattttt ggattattca
    18361 gtgtagcacc aatacgcgct cttcgcacaa ttgctagtat ttatgtcgat atcatccgag
    18421 gaattccaat gatggttctt gcgttcttta ttttctttgg gttgcctggt atcgttggat
    18481 ttacgattcc tgacttcctt gctggtgtta tcaccttgac tcttaacgca agtgcttata
    18541 tcgcagagat tgttcgaggc gggatcaacg ccgttcctgt cggacaaatg gaagcttcca
    18601 gaagtttagg tctttcttat aatcggacaa tgcaaaaaat cattttgccg caagcgatac
    18661 gcatcatgat cccatcattc gtcaatcagt tcgttatctc attaaaagac acaacgatca
    18721 tttccgcaat cggtgtagtc gaattgctgc aaactggtaa gatcattgtt gcacgtacaa
    18781 cacaaagtac gtatgtttac ttgatcattg cgatcatgta cttgattttg atcacagcat
    18841 tgacaaaatt agcaaaagtt ttagaaaaga aggtgaagta agatggcaga aaaaatctta
    18901 gttgagcatt tagtcaaaaa atacggcgat aacacagtat taaacgacat caatgtgtcc
    18961 atcaacgaag gagatgtcgt ctgtatcatt ggcccttcag gttcaggaaa aagtacattt
    19021 ttgcgttgct tgaatcgttt agaagaacct tctgacggtg aaattattat tgacggcgcc
    19081 cacttgatgg ataaaagcac agatatcaac ttggtgagac aacatatcgg aatggtattc
    19141 cagcacttta acctattccc tcatctttct attttggaaa acatcatttt agcaccgatg
    19201 gacctgaaga aagaatctag agaagaagca gagaaaaaag caatcaaatt gctgcagaca
    19261 gttggtctag aagataaaaa agatatgtat cctgaaatgc tttccggtgg acaaaagcag
    19321 cgtgtggcta ttgctcgtgc tctagcgatg aaccctgaca tcatgctttt tgatgagcct
    19381 acatctgcac tcgaccccga aatggtcggt gatgtcttga acgtcatgaa aaagttagca
    19441 gaacaaggta tgacgatggt tatcgttacg cacgaaatgg gcttcgctaa agaagtggcc
    19501 aatcgtgtca tgttcattga cgacggaaac tttttagaag acggaacgcc tcaacaaatc
    19561 tttgaaaatc cgcaaaatga acggacaaaa gatttcttga ataaagtatt aaatatttaa
    19621 taaaaaccaa aaatgcccgg tcgcttcatg acgaccgggc atttttagtt tcttgttttt
    19681 tcacttaact gtaccagccg catcgcttgt tctcctgcac aaacaagcaa tcgctctacc
    19741 tgttccatcg cataagaaag taccatcggt tcattgacga tatcaagtac atagtcgata
    19801 ccaccttcag aaactatgat gattatctta ttctaaagct ggcttttttg ttggtacaaa
    19861 catgacaact acaaaaaaac caggcgaacg tatgattgtt cgtctggttt ttaattatta
    19921 atgacttagc gtttttacta gaaatagaaa atttgggcaa gtctacttta tatttttact
    19981 agaaaaacta cttccgcttt ttcccgccaa aagcatcttt aaacttatcg aagaaaccgt
    20041 cttcttgctg ttcttcaact ttgatgccgc cagcttcagc gaatgcacgt aatgcttctc
    20101 tttgttgttc atttagattt ttcggtgtga tcagttttac tttgacatgt tggtcgcctg
    20161 ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa acggaagttc gtacctgtct
    20221 gtgttccagc agggatcttc aatttcacat ctccatggac agttggtact tggacttcat
    20281 cccccaaagc tgcttgtaca aaacttaaag gcaattcata gtaaatttca gaaccttcac
    20341 gatcaaagat atcactttct tctacacgaa aaaccacata caaatcacca aatggtccgc
    20401 cgttttctcc agcttctcct tgaccagcta aacgcatttg ttgtccttct tctacaccag
    20461 ctggtacatt cactttaact gaatgtgctt tcttctcatg tcctgagcca tgacaggttg
    20521 gacaaggttc tttgatttct ttccctgtgc catgacaaac atcacaagtt tgacggctca
    20581 tcactcgtcc aagtggcgtt tggcgttcta cattgatcgt acctgcacca tgacatttat
    20641 gacaagtagt tggttgtgtg cctggttttg caccgtttcc accacaagta tgacagattt
    20701 cttcccgatt atacttgatt tctttttcta caccaaaaat cgcttcttca aaagacaagt
    20761 cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt tggatcgact gaccgaccgc
    20821 ctcctccaaa gaaagagtcg aagatatctt caaaaccacc gaagccgcca ccagagaagc
    20881 cgccgaatcc accaaatccg ccaccagctc caccagcacc gtaatttgga tctgtgcctg
    20941 catgcccgta ttggtcgtat gcagcgcgtt tttgcgggtc acttaaaatc tcatatgctt
    21001 ctgatacttc tttgaatttt tcttccgcat caggttcttt attgatgtca ggatggtatt
    21061 ttttcgaaag cttacgataa gctttcttta tttcatcttc tgatgctcct tttgagagcc
    21121 ctaagacttc ataataatca cgttttgttg ccataggctt tcctcctatt ggttctcatc
    21181 attttcacgt cgcttgttat catatcatat tttgtttgaa acaaaaaccg atacttcaaa
//
LOCUS       DAXWLB010000035        20923 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXWLB010000035 DAXWLB010000000
VERSION     DAXWLB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.035"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(607..780)
                     /locus_tag="ACGE4H_000998"
     CDS             complement(607..780)
                     /locus_tag="ACGE4H_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364432.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(783..1181)
                     /locus_tag="ACGE4H_000999"
     CDS             complement(783..1181)
                     /locus_tag="ACGE4H_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364433.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1397..2452
                     /locus_tag="ACGE4H_001000"
     CDS             1397..2452
                     /locus_tag="ACGE4H_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364434.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2472..2825
                     /locus_tag="ACGE4H_001001"
     CDS             2472..2825
                     /locus_tag="ACGE4H_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364435.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3007..3294)
                     /locus_tag="ACGE4H_001002"
     CDS             complement(3007..3294)
                     /locus_tag="ACGE4H_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364436.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3307..4125)
                     /locus_tag="ACGE4H_001003"
     CDS             complement(3307..4125)
                     /locus_tag="ACGE4H_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8364437.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4351..4572
                     /gene="relB"
                     /locus_tag="ACGE4H_001004"
     CDS             4351..4572
                     /gene="relB"
                     /locus_tag="ACGE4H_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8364438.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4574..4840
                     /locus_tag="ACGE4H_001005"
     CDS             4574..4840
                     /locus_tag="ACGE4H_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8364439.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5007..5492
                     /locus_tag="ACGE4H_001006"
     CDS             5007..5492
                     /locus_tag="ACGE4H_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364440.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            5701..5883
                     /locus_tag="ACGE4H_001007"
     CDS             5701..5883
                     /locus_tag="ACGE4H_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8364441.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5986..6270
                     /locus_tag="ACGE4H_001008"
     CDS             5986..6270
                     /locus_tag="ACGE4H_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364442.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6286..6522
                     /locus_tag="ACGE4H_001009"
     CDS             6286..6522
                     /locus_tag="ACGE4H_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364443.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6544..6798
                     /locus_tag="ACGE4H_001010"
     CDS             6544..6798
                     /locus_tag="ACGE4H_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364444.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6880..7065
                     /locus_tag="ACGE4H_001011"
     CDS             6880..7065
                     /locus_tag="ACGE4H_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFF8364445.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7132..7527
                     /locus_tag="ACGE4H_001012"
     CDS             7132..7527
                     /locus_tag="ACGE4H_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8364446.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7544..7894
                     /locus_tag="ACGE4H_001013"
     CDS             7544..7894
                     /locus_tag="ACGE4H_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364447.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            7916..8290
                     /locus_tag="ACGE4H_001014"
     CDS             7916..8290
                     /locus_tag="ACGE4H_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364448.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8597..8995)
                     /locus_tag="ACGE4H_001015"
     CDS             complement(8597..8995)
                     /locus_tag="ACGE4H_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8364449.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9187..9954)
                     /locus_tag="ACGE4H_001016"
     CDS             complement(9187..9954)
                     /locus_tag="ACGE4H_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8364450.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(9989..11149)
                     /locus_tag="ACGE4H_001017"
     CDS             complement(9989..11149)
                     /locus_tag="ACGE4H_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFF8364451.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            12193..12996
                     /locus_tag="ACGE4H_001018"
     CDS             12193..12996
                     /locus_tag="ACGE4H_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8364452.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13581..14036
                     /locus_tag="ACGE4H_001019"
     CDS             13581..14036
                     /locus_tag="ACGE4H_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364453.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14155..15213
                     /locus_tag="ACGE4H_001020"
     CDS             14155..15213
                     /locus_tag="ACGE4H_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFF8364454.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15255..17204
                     /locus_tag="ACGE4H_001021"
     CDS             15255..17204
                     /locus_tag="ACGE4H_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8364455.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17207..20848
                     /locus_tag="ACGE4H_001022"
     CDS             17207..20848
                     /locus_tag="ACGE4H_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFF8364456.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
BASE COUNT         7326 a         3294 c         3702 g         6601 t
ORIGIN      
        1 tcaatcactc ataaaagtat gcgctttcat ttttttggat gagattaata tcaataaata
       61 acgatgtaaa aaccattaat tatgctcttt tataaggata ttaattaatc taatttgaaa
      121 aaataattca aaatttaaat gttttaatat tacaaataaa atcatgttat cgtaaaatgg
      181 tataaaaaag ctaaatattc tatttttgtt attttattag cagggaaatt taaatagttt
      241 acaagaatcc gtagaaatat attggttttg ccttcagatt aatactggat catattaatc
      301 tgaatataat atatgaacta ataaatagct taacctcgct attttgtttt ttctgatcac
      361 tatctataca gcattagtaa atagaatttt aaaaataaaa aaattaagac aaaaaaagta
      421 tcaacataaa catatacata tacacgttta tgttgataca cttaatttgt cctttaaaaa
      481 gcacttattt taatattatg ttattttata atcttattac aagctgattg ttagacgttg
      541 tcctttcatt acatttatta acggttattt tatcgtttaa tttattaatt atttttgatc
      601 taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg cgatcattgc
      661 taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg cgattaagca
      721 gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta gcatactcat
      781 aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac agcatcaatc
      841 aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga tgaaaagata
      901 taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa tttaatacca
      961 tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact ttcttctaaa
     1021 gaatcaaaat atttttcgac agcaaccttt gaagctttta ctattgaagc atttcccttt
     1081 tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt aaaatcttct
     1141 aaaataatag tagtgtaatt cttatttctt ctcaatcgca tcaggatcac tccttaatat
     1201 aatttatgct tttataatat cataataaga taaatttgca agtgtttatt tacacctttt
     1261 tatttacact ttattgtaat tgattatact tttttgattg catttttttc ttataaacag
     1321 ttgcaactat tagtaaatag gtgtaaacta ttttcgtacc gaaataccat aaacacaaac
     1381 agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag ggaataaatc
     1441 agtaaaagaa atgagtacac acgtttctcc actctcgctt ccctctcgta ttttgaacaa
     1501 aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac gatcactaaa
     1561 cgtttctgaa tatagcctag taaacactac tgaaagctat tattttggga aggatataca
     1621 cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca gcagatacaa
     1681 gcaggaaacc taccaagata tcctatcttt ctctttagct aacctagcac gtttagaaga
     1741 tcaatctgat gacgttttaa ccgttaatct agttttaggt ctgccttcat ccgactatac
     1801 cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag ttgaagttga
     1861 cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc cgattggaac
     1921 actatataac ctcctccttg ctgatgatgg tagtgtacaa gatgaatcat tgatcagtca
     1981 gaagatcggt tttgttgata ttggtggtgg cacgaaactt tttgatgttg tccaaaattt
     2041 tgaaattgac gaaaacaacc gtgaacaaaa ggataccggt atttttactt tatataatcg
     2101 tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg aaaagattat
     2161 ccgagaaggc aaagaaaaag gcgtatatct atttaaacca agcggaaatg aaagttttga
     2221 tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg ttgcagaagt
     2281 tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg gtggtggggc
     2341 ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt ttgttgaaga
     2401 cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat aaaacagcga
     2461 aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc aatagagaaa
     2521 aggttagcga gttaccttta gcagagtttc ttgaatctga atatgaaaca ctaggcgtta
     2581 aatactctca gacgatcaaa aaagctttga atctatatcg gcgtgtaaag aacaatgaag
     2641 ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagctgaa gtaaagaata
     2701 aaccaactga tcataaccct attcaacaag cgcatccttc aacggttaca tccgatcatt
     2761 cagcaaagaa agaaaagcca atgctaaaag ggttcaatgc accaagtaac aaagacttac
     2821 tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac aaatacaaac
     2881 ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat aaacacgtat
     2941 acgaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg atcagcttta
     3001 aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac caagtaaagc
     3061 tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc tttgtgatgg
     3121 ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta gtttagctac
     3181 caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt caaccacttc
     3241 aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt tcacttcaat
     3301 cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg tgctttcaaa
     3361 gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa tattcttacc
     3421 ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat aattagataa
     3481 cgttggtacc aattctagga actcacgact tgaattagta ttatgtttga tcctgctacc
     3541 gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa tcaactgttt
     3601 tgctagtgca tcaatatctt taccaagctt aagcaaagct tcaaaaccat ctttagatgg
     3661 atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta gtagacctgc
     3721 atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt catagttaac
     3781 aacgatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt gcatctcaac
     3841 ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttatag ctaaaggttc
     3901 agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt tactgaaagc
     3961 atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca gcaccttcat
     4021 acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt tacctgttcc
     4081 acctttttca atgtttccag taatgattga caacttcttt tccatgatta aacctccctt
     4141 attaatcttt tgtaactcca ttatacacta taaacacgtt tatgtatata tttatataca
     4201 taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata tatatgtata
     4261 tgtttatgtt tatgtatata ttcaaatata taactattgc atttgtcata caatcgtagt
     4321 acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg tgtatctaaa
     4381 gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat ttctgaattt
     4441 atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac ctacaagcaa
     4501 ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat gctaaaagag
     4561 ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa aagcgctaaa
     4621 aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa atcttgtagg
     4681 cactgatgat ccaagaaagc atggtaaagg tctaacagct aataaatcag gctactggcg
     4741 atatagggta ggtgcatacc gtataatagc tgatattagc gagcatgaag tgacaattct
     4801 tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct gataaggctt
     4861 gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta cctaaaaaag
     4921 aacaatatcc tcttgatatt atacacacca gtgtgtataa tataagtgta aacaagctaa
     4981 ttaactttca aaaggagcga ttttttatgg gatggtatgg tgattattca tcagcaaatg
     5041 aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta ggtaccaaag
     5101 taacgaagac ctatggaatt atccttttca aagatgcaga aggaacctac gatattgaca
     5161 gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac agtcctgctt
     5221 actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa gaaaaaatgg
     5281 ttaagctaca actagataga attaaagaag aaagcaataa accaaaactt gatgacttat
     5341 tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtc tatcagtata
     5401 aactcaaacg tagctttatt tttcgactac aagatggatc actcactcgg ttcactcgac
     5461 tatcagcagg agatgttaag gaagtaacct aaaacttaaa tacataaaca taaacgtgta
     5521 tacgtttatg tttatgtata tgtatatgaa tataaacgta ttaggtacga acacttttaa
     5581 attcttttct gggctttgtt tttagggtag ggctaaaaaa aacatcacca tctgatccaa
     5641 aacagctcag aaacgcattt aaacgtatct gataccaata ttacagaagg agtgtatcgt
     5701 atgattaacc tatttctttt agtgacagct agtacattat tctttactgg aatatcactg
     5761 ataatttcct atttgatcta taagaaagcc gaaaatcaca caagcaaagc aatcggacta
     5821 gccgtttcat cctttttaac tttgctgact ttatttgtta ctttatcgtt ttcttttgtt
     5881 tagaacagct gatccattag cacctttttc tctttacatt atacacactg ttgtgtataa
     5941 tgtatttgta aacaagctaa ttaactttta ggaaggagtg acaagatgct aacagctact
     6001 ttacagcaac ctactcacgt tgctattgca gaaagttata caattaatgc agggatcgtt
     6061 aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcggcaagc gtataacaga
     6121 cagctaaaga aagctattct aaagcacaaa gattccttca tcgggaactt ggaaagtgta
     6181 gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga cggtcgcttg
     6241 atttctctat ttgcttttgg aattaattga tagggagtgt gaactttgga aggtaagact
     6301 tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc gataacttct
     6361 gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct tgaatgcgtt
     6421 tggatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt ataccacaat
     6481 ggtgccgagt gggatgcaat tggaaatatc catttttctt aatataagaa aggagaacac
     6541 aatatgaatt ataccgatga agaacaaatc gctttggaat acgttttcac aaatgaagaa
     6601 ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa ggctttggct
     6661 tatgaaccat acgaatcaac cgtaactgtc attgaattct tagataccta tacttctaat
     6721 gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc tgaaaaaacc
     6781 aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga ttccatacac
     6841 aacaatgtgt ataatagatt ttgtaaaggg agggtagcta tgcctaagac agcaagagaa
     6901 attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg cagtcatgca
     6961 aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa tggtgatgta
     7021 ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc ttgccttcct
     7081 atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga aatgaagaca
     7141 aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa catcgttttc
     7201 cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata tttaaacgct
     7261 aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc taaaccatca
     7321 gcagtggaag aattaacttt gaaagccaac caagtggtag taatcatcca actaaacatt
     7381 aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag tgttccggaa
     7441 tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact tcaaaaagca
     7501 ctagaagaag agttgcttga aaagtaaaaa ggagaccgaa cctatgaaag ctacacactt
     7561 aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg gcattagcgt
     7621 tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact tgcgaattga
     7681 gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata cgcacactga
     7741 aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat cactaacgaa
     7801 cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg actaccacaa
     7861 tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga gaaagatgaa
     7921 acaaacacaa acagaaaccg ttaacgatac gcttgctgat caattagcag gttattataa
     7981 attatttgag atgggtaaag cacagcgctt gatccactat ctttggaaat acaaaatttt
     8041 gtttcttttt tgggaatcaa cactaacatt ctcagttctt ttttataagt cagattttta
     8101 taatcaacac ttagttttag ggagtttttt gcttatctac accattttga tgcttaattt
     8161 atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga tcgagagaaa
     8221 catcaaaagg gtatccttca agatcacgtt aaacaagcct accgataaaa agctctcaga
     8281 agcgttctga gagcttttga cattttcttg tataatttta tcctaacaca ttacaaagca
     8341 cctaattcga gcttatatca agcaaatttg ctatttatta ctcaatacat atctaaaaat
     8401 tacttgatgg aaaattagat acatggtaag catgaaaaga aaatcaattt gagcagatgc
     8461 agtacaatac atagcaattt aaaagctctc agaaggcttc tgagagcttt taatatattc
     8521 tttgataatt tgtctttctt aaatccatac gtcctaaaac aagctgtttt tgcttaatat
     8581 gatcatctga gagacatcaa atcaactttt ctctcaacaa atcattcaaa gctttcgaca
     8641 gattaatatt ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc actgattttc
     8701 gatcataatt ttgcttgttt cgagctttag caactttcgt attagctttt acgacaaata
     8761 aattaacatt ctcagatcgc ttgattcttg catttaactc ttgaacaggc gtaggcgatg
     8821 gaatttcttt ttcttgcaac tctaaaagca ccaagtatcc ttccaatact tcttgcgcca
     8881 tctcaaaagc atcttctaaa gtttcaccat gtgtatataa gtctggaaaa tctgggaatt
     8941 taactgcata ttcatttcct ttccattcga atactgcaca aaaaaagtat ttcatcgcat
     9001 tgcacctctc taattaaaaa ataatttttg cttttttgtt tcaaaaaaaa gaatcgccta
     9061 ccaaaaaaat tgataggcga tcacaagcta attaacctct aaaggagatt aataccttgc
     9121 tcccaaggca ctatatatct tagcatacgt ctgaagcatc ttcaaacgag taagatatat
     9181 tttgcattaa cctaattttt tttctctata agttttttca tcaaactcac tagaaatatt
     9241 aaactggaca gcaaaattgt aatttctgcc ttttctaact ttagcaaaat caatattcag
     9301 cattgatttt tcattaattt cctcaactga ttcccttaaa acacgtctat caaaattcga
     9361 tattgtttta tactcatttt ctgttttcgt aatctcacga attttaacta tttcaatcag
     9421 gggattctta tatatttcag ctttctcaaa atctccttta tttttggcga taagatattt
     9481 tttataatac atcgccaaat atttgtataa cacgatgcta tatttttttt gaaaagatgc
     9541 taagtcttca aaaacaaaga acggatactt ctttttgatt gagacaatta agggaattac
     9601 atcttctgaa aaactgaaag atacactatg ctcttctaaa tcccaatgaa tccgatcaat
     9661 agcagataca gtagtatacg tcttatattt tttgtctaga ttcgaaattt cataaactgc
     9721 ttgtctttgc aaagtgttaa ctatggtttt tagttccgtt tcaagatttt gatcaacatc
     9781 catgtaatta ataatcgctt taacaggtaa atctactacg cctgttttct ttagctttgt
     9841 gggtgagata taactgaaca gcaattgata aagttcatac gactttaaat ccattttcat
     9901 gtcaattgtt gctaagaact cctcattgta ctttgtcagt tcatcttttt tcaaaagttt
     9961 ttccttcctt cacgcatttt ttcagttgtt aatcataaag gttcacataa ggcaaataac
    10021 tcgtaattga tttttcgaga tatttaacag tattttttat tgtgtaatcc tccttatgag
    10081 tagcaacgta agataggtga ttgacaactg attcaaatcc tctatctttg gataaacgat
    10141 cataaagcgg ataaacttta ttctgaagtc ggctcataag tttaagatcg cataagtcgg
    10201 cagaactgat caaaaagttt ttgattagta atttagtgta tttgctattc atagctttca
    10261 agtacgattg attcattttc tctgttttat cttctgcgcc ttttaaataa gcagggtctc
    10321 tttgttcttg cttataaggt accggaactg acatggtacg tgtaagcaag aactttatgt
    10381 ttgtataagt atgacccttc ttgataagct catagtcaat tttaaaatca gtgtatttat
    10441 tcagttgggc aatcggttct ttaattacac gtctttcaaa gtctcgaaaa gctttatatt
    10501 ttttctcaat actaaatttc tttcgtaaat ctgcaatgga gaaaacagga ttttttaatc
    10561 tatcaagtga ttttgtactc catgttaagt tatctctatt tgcttcatac agattaaaat
    10621 ctttagtgaa taattgaaaa aaactgatcg catacttgct tgtcaagtta actacttgat
    10681 caaatgtata ttgcgtaaaa tgattttcta agttcgttaa taaaggcata attgaactac
    10741 taaatttaat agatactgca tcagaaaaat cattgaattc agtaatttct agcggtgaga
    10801 tgactttgta ttcagtctta ccgttaggtt tagttctttg aattttaatg acagattgct
    10861 gatgcaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat gaactagaag
    10921 acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa ggcggatttt
    10981 tagggtcaag atgtcctata gcgtaaagat aaattttttg tggcatagca tccatttcaa
    11041 caatgctatc tacaagttga ttcgataaag taacagttaa atcaaacttt tttagattgc
    11101 tttcggtagt ttcaaatttc gggtttacat tcttttctgt tattttcata cgattgcctc
    11161 ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc attgaataag
    11221 tatagacctg ttttcgatac gttacaataa gaaatgtatc gtaaatttat aacataactc
    11281 aatgtcagta aaacacattt tattttttcc aatcaaattt cattgaaaat acagcagaaa
    11341 tcgctatttt agaacatttt caaaataaaa atccttttca ataaaatacg aaattataaa
    11401 aattaccaaa attaaaaaac aaatattttt ctagttttta gaaataaatt cacatacttt
    11461 ttcttttttt ggatatattc ggaataccac atcgcttttt gtccacgttt gcatcgcttt
    11521 ttgtccacat tcacatcgct ttttgtccat gttcgcatcg ctttatgtcc accctaaaat
    11581 ctatttaaga ttgatataaa gcctttttgg aggttgctaa agttttaaag attaaaatat
    11641 atattataaa tataaataaa aggggaagct gaagcttcct ccttttcctt tttttcaatt
    11701 attcaaagtt acacaagcaa aataaactcc tagatagtgt tagttttttt taagattttt
    11761 tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata tctgttaata
    11821 tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt gggagagtaa
    11881 gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa gtggacagta
    11941 agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta tcacactagg
    12001 ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa attagattag
    12061 taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa caagcagaaa
    12121 aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa aaagaagatt
    12181 ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga tgtagattta
    12241 gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac aacagctttg
    12301 cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa aatgaaacgt
    12361 caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt caaatacgac
    12421 agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa actaggtaat
    12481 ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt tcaaaaattc
    12541 cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt tgaatttatc
    12601 ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac aaaactaaga
    12661 agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa agagtggcga
    12721 aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat accttcaagt
    12781 tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga atttttagct
    12841 cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat ctatgcaaag
    12901 aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc aaacttgcca
    12961 accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg taattttaat
    13021 acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg caagaagttt
    13081 ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct atacaaattt
    13141 taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt tcttaatttg
    13201 ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg tgatatcagt
    13261 tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt ggacaagtca
    13321 actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc gtctgataaa
    13381 tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa atagctaaaa
    13441 taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg ctgattgatt
    13501 tatttatgtt attatgatat tataataaca taaatgtgtt acgcccgaac ctcattttaa
    13561 aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt aactatatgg
    13621 agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag catgggttac
    13681 cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt attaagctga
    13741 gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat gaactaaaag
    13801 gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt attggaaaat
    13861 atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct actccaatgg
    13921 ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca atagcaaaaa
    13981 tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga agataatttt
    14041 tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg aacctagaat
    14101 taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa actaatggaa
    14161 aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg gttgcttggt
    14221 gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt tctaggagtg
    14281 ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta taattattat
    14341 ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc aggagcagag
    14401 atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt aaaactcggt
    14461 tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa cttatatatg
    14521 gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat gttaagtact
    14581 gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta tcaagttttt
    14641 attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga taccaaagtc
    14701 aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat gaatgatgtt
    14761 aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac tcctgataat
    14821 atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg aagctttctt
    14881 accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa gaataatgtt
    14941 gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa aattgaagct
    15001 acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga ttcgcaagaa
    15061 agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa tgatacacaa
    15121 aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg agacatgaag
    15181 acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat tttagaggaa
    15241 gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa actgaattta
    15301 atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt gatgacgaac
    15361 tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc ttgttaggtg
    15421 aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag cgacaagatg
    15481 gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg ggagatgatg
    15541 tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag cgaattttaa
    15601 aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat aaagttgtta
    15661 gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg ttagcaaatg
    15721 aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat atcaaaagag
    15781 atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa agcgatgttg
    15841 tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat cctcaggctt
    15901 cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta caagcaaaca
    15961 agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg gaggatttga
    16021 ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg caaatatttg
    16081 gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa gaggaattaa
    16141 agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt aaacgtatga
    16201 agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct ctgtttacag
    16261 gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga ctttatggcg
    16321 atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat gatgcaatgc
    16381 gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt attgtgtgtg
    16441 atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt ttagatgaag
    16501 gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac actgtttcat
    16561 tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc gattcgttaa
    16621 acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa tatagcactg
    16681 aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg attggtgaag
    16741 gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg gctttatccg
    16801 aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa ttagtacgaa
    16861 aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat ggtcaaaatt
    16921 tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa gaggattcta
    16981 aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa gaagcgatta
    17041 ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg agtgaagccg
    17101 gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct tccgttgctg
    17161 aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga aattcaagaa
    17221 atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag ttattggagg
    17281 agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc ctgacaatca
    17341 agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa gtaaaccggc
    17401 agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag aagttaatga
    17461 tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca gtgccgattt
    17521 cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg caggtactca
    17581 actagcatct actttagcaa cttatagtta ctacggatat attgaaacag tctcaggaaa
    17641 tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga ttggtagttt
    17701 tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg aactccttgg
    17761 aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag gtggatcgga
    17821 tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg gattaaataa
    17881 tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa tactaggtta
    17941 tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat caccagtact
    18001 tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg caggatcgct
    18061 attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata taccaacaaa
    18121 tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa atcttgctcc
    18181 atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa tcaagttcgt
    18241 tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca atactagcag
    18301 ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc tattaaaaga
    18361 atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta atgtcgccga
    18421 aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt taggggatgg
    18481 aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt ggtcagcaaa
    18541 tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc ataactttga
    18601 tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt cggtagcttt
    18661 attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata atatcgctcc
    18721 ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat ggcgtacagc
    18781 atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga tgaacgctga
    18841 atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca atgtgaatat
    18901 cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa caggatcact
    18961 tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat ctctgatcgg
    19021 actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata ctgctgtggg
    19081 attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat ctgctatgat
    19141 tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata atttgattaa
    19201 tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg ctaagttatt
    19261 atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag caatcaagaa
    19321 aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa aagatatggt
    19381 tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga acaatatggg
    19441 agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa caccagaagg
    19501 aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt tcaaagattt
    19561 agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt taggacgtat
    19621 ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag atagtaaata
    19681 cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg atgaagatgg
    19741 aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta atattggtat
    19801 ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag atggaaatct
    19861 tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata gtaacggacg
    19921 tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg atgagtttgg
    19981 taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag tcgatgagga
    20041 tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag aactcgaaca
    20101 acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg agattacttt
    20161 agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt cagacagagt
    20221 gcatcgggaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc ttaacagcaa
    20281 ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat cgctaacaaa
    20341 agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc ctaatgttaa
    20401 tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca atcaatcaat
    20461 tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat ctacttcaga
    20521 taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat cagccgttga
    20581 atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt tagcaaaagc
    20641 tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta ttaaaatagg
    20701 taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa ctggtaaagt
    20761 agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta atcaaaaatc
    20821 aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct ccttcttttt
    20881 actttgattt ctaactgaag atgtcaagga aaatttctat tta
//
LOCUS       DAXWLB010000036        20912 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXWLB010000036 DAXWLB010000000
VERSION     DAXWLB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.036"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(807..1559)
                     /locus_tag="ACGE4H_001023"
     CDS             complement(807..1559)
                     /locus_tag="ACGE4H_001023"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFF8364457.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1672..2592
                     /locus_tag="ACGE4H_001024"
     CDS             1672..2592
                     /locus_tag="ACGE4H_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8364458.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2665..3600
                     /gene="murB"
                     /locus_tag="ACGE4H_001025"
     CDS             2665..3600
                     /gene="murB"
                     /locus_tag="ACGE4H_001025"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFF8364459.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3898..5376
                     /locus_tag="ACGE4H_001026"
     CDS             3898..5376
                     /locus_tag="ACGE4H_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364460.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5653..7713
                     /locus_tag="ACGE4H_001027"
     CDS             5653..7713
                     /locus_tag="ACGE4H_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFF8364461.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7796..8365
                     /locus_tag="ACGE4H_001028"
     CDS             7796..8365
                     /locus_tag="ACGE4H_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364462.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(8540..9346)
                     /locus_tag="ACGE4H_001029"
     CDS             complement(8540..9346)
                     /locus_tag="ACGE4H_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364463.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            9484..10998
                     /locus_tag="ACGE4H_001030"
     CDS             9484..10998
                     /locus_tag="ACGE4H_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFF8364464.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(11401..12384)
                     /locus_tag="ACGE4H_001031"
     CDS             complement(11401..12384)
                     /locus_tag="ACGE4H_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFF8364465.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(12389..13036)
                     /locus_tag="ACGE4H_001032"
     CDS             complement(12389..13036)
                     /locus_tag="ACGE4H_001032"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8364466.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(13121..13753)
                     /locus_tag="ACGE4H_001033"
     CDS             complement(13121..13753)
                     /locus_tag="ACGE4H_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8364467.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(13769..14320)
                     /gene="coaC"
                     /locus_tag="ACGE4H_001034"
     CDS             complement(13769..14320)
                     /gene="coaC"
                     /locus_tag="ACGE4H_001034"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFF8364468.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(14317..15054)
                     /locus_tag="ACGE4H_001035"
     CDS             complement(14317..15054)
                     /locus_tag="ACGE4H_001035"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFF8364469.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(15051..16040)
                     /locus_tag="ACGE4H_001036"
     CDS             complement(15051..16040)
                     /locus_tag="ACGE4H_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFF8364470.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16298..16840
                     /locus_tag="ACGE4H_001037"
     CDS             16298..16840
                     /locus_tag="ACGE4H_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8364471.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            16857..17942
                     /locus_tag="ACGE4H_001038"
     CDS             16857..17942
                     /locus_tag="ACGE4H_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364472.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            17954..18760
                     /locus_tag="ACGE4H_001039"
     CDS             17954..18760
                     /locus_tag="ACGE4H_001039"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364473.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            18761..19588
                     /locus_tag="ACGE4H_001040"
     CDS             18761..19588
                     /locus_tag="ACGE4H_001040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364474.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            19585..20658
                     /locus_tag="ACGE4H_001041"
     CDS             19585..20658
                     /locus_tag="ACGE4H_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364475.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         6873 a         3673 c         4182 g         6184 t
ORIGIN      
        1 tttaacaaaa aacatagaat tttttatgta atatttgttg agaaaagcta gtttttgaac
       61 tcattccttg tgatttggag ttagatgggg atcatgactt ctcggtatta agtcaaattt
      121 atgcaaagtt gtgaggaacc attttcaaaa aaatttctta cgactcgaaa gtaggcgact
      181 ttttcacaac ttctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      241 aatagcaaaa tatgaggaac tattttgcca tttgtttctc taatactcag gtcaggacgc
      301 tgatttcaca acttctcaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
      361 accaaacgaa aaatatgagg aactattttt gtttggctgt tcttattact tgatgcagtg
      421 cagctttttc acaacctctt aataaacggt gttcaaaagc ttgcatctgc ggtaataagc
      481 ccaaccaaac gaaaaatatg aggaactatt tttgtttggc tgttcttatt acttgatgca
      541 gtgcagcttt ttcacaacct cttaataaac ggtgttcaaa agcttgtatc tgcggtaata
      601 agcccaacca aacgaaaaat atgaggaact atttttgttt ggctgttctt attacttgat
      661 gcaggacagc tttttcacaa tctctcgata aacagtgttc aaaagccagt ttctcggtat
      721 tatgccaaat ttatgcaaaa tgtggaaagc cattttcaca aaatttttct taatactcga
      781 aactaaacga ctttttcaca acctctttat ttcaaatcta attctactgg acaatgatca
      841 ctgcccataa tgtccgtatg gatctttgca tcagctagtc ctggctctaa atctttgctc
      901 gtcaaaaagt aatcgatccg ccaaccagca ttgtttttcc ttgaattgaa tcggtagttc
      961 caccaagagt agacaccttc ctgggttgga taaaaataac ggaatgtatc gataaacccg
     1021 ttatccaaca gtcgagaaag agccgctctt tcttctggag tgaatccagc atttttttga
     1081 ttcgttttcc agtttttcaa atcaatcttt tgatgagcga catttagatc cccacacacg
     1141 atgactggtt tttgtttttt tagattttcc aaataattat aaaaggcttc ttcccattct
     1201 aatcggtaat ctaaacgttt taattcgctt tgagaatttg gcgtgtaaca ggttacaaga
     1261 aagaaatcgg aatattccaa agtaatcaga cggccttctg tatcatggat atcgataccc
     1321 attccgtaag tggcattcaa tgctggtttt ttagcaaaaa tcgctgtgcc ggaatagcct
     1381 tttcgttccg cgtaattcca atattgataa taacctggaa gatctaaatc aatttgtcct
     1441 gcttggagtt tcgtttcctg cagacagaaa aaatcggcgt ctaactcatg aaaaacttct
     1501 aaaaaatttt tatttacgat ggcacgaagc ccgtttacgt tccaagagat aaatttcaat
     1561 aaaagcacac tccttcgaat taatttcctt acctttgcga tactattgta cctcatttta
     1621 gtaaaaaatg gtgtaagatg aaagtaacta aatgatccag gaggaaagta gatgaagatt
     1681 ggtgtaatcg gtgtagggaa tatcgcagaa aaagcatatt taccaactta tgcgaaaaaa
     1741 caaggaacac ttgattttta ttttgcaaca agaaataaag aaacaaaaga aagaattcaa
     1801 aaagagtacg ggttccaaca tctatatgaa acgatcgatg agcttttaga agaaaaaatc
     1861 gaagcatgta tgatccacgc agcaacaaaa gtacattatg aattagccaa aaaatgtcta
     1921 gaaaatggta tccatgttta tattgacaaa ccactgagtg tccatctaaa tgaagttcgt
     1981 gaattgcaag aattagctga taagaaccaa ataattttga tgatcggttt caaccggcgc
     2041 tttgctccta tggtagaaga attgaaacgt atcccagata agcggttgat ccacctgcaa
     2101 aaaaatcgaa tagcggcaaa agaaacgaca gagttcgtcc tttatgattt gtttttacat
     2161 cttattgata cagctgtgta tttattagat gaacccattt tgagaacaaa ataccagata
     2221 agagaaacaa aggaattttt ggaatatgcc attttacaat tagaaacaca aaatcaaacg
     2281 gctattttaa cgatggattt agcaagtggc gctaatatag aaaaatatca agtaacaagt
     2341 aaaaatggta cttatgaagt aaacgatctg acccagcttg tcattcaaga tgctgatgga
     2401 aaaaaagtga aagaatttgg cgactggaca aacacgctag tcaaacgcgg gtttgagcca
     2461 atggtagagg ccttcttttc tcagatccgt ttaggtaaat cggaaacaga attgcttaaa
     2521 caaaaagaag ttgtaagaag tcatgaatta tgtgaggaag tgcttaaaga tcactaccgt
     2581 catcaacttt gaacaagact ggcaagattg tattaagcgt gatagaataa tgaggacgtt
     2641 tgagaaaaga aaggaaaatc ctcagtgaat aaagaagaaa tagttgaaag cttagccacg
     2701 attactatat tgtttgatga accattgatg aactatacgt ttacgaaaac aggtggacca
     2761 gctgatgtcc ttgcattccc gaaaaaacaa gaagaagtaa agcaaatcat cgattattgc
     2821 cgaatccatg atataccttg gatggtatta ggaaatgcaa gcaatctgat cgttcaagat
     2881 ggcgggattc gcggtgtagt catcatgctg actgaaatga aacagattca tgtcaaagga
     2941 acgatggtca ttgcggaagc aggtgcctca ctgattgata cgacttatgc agctttggca
     3001 gaatcattga ctggatttga atttgcttgc ggcatcccgg gaagtgtcgg tggagcggtt
     3061 tatatgaatg caggtgcgta tggcggagag atcaaagatg tttttgctga agtggatctt
     3121 ttgcttgaag atggaacatt gaaaactttg acaaaagacg aaatggtatt ttcttatcgt
     3181 cacagtaaag tccaagaatt acgtgctatc gtattggaag ctcgattcag tttgcaaaca
     3241 ggggattatg aagcaatcaa agcaagaatg gatgagttga ccgaattgcg tcaatcaaaa
     3301 cagccattag aatacccttc atgcggaagt gtctttaagc gcccagtagg acactataca
     3361 ggtcagctga tccaacaagc aggattgcaa ggcctgaaat ggggcggtgc acaagtctct
     3421 gaaaaacatg ctggattcat cgtcaatatc gatcatgcga cagcgacaga ttatattgaa
     3481 ttgattgcgc acatccaaga agtaatcaaa gaaaaatttg atgtgtcttt agaaacagaa
     3541 gtgcggatca ttggagaaaa acaagcaata tcagaaacaa gcagaagttt agccaattaa
     3601 taagtaatgg aacttaaaat aagtaaaaga gcaggacggt ggccgtcctg ctcttttttg
     3661 tggttgttta cgcaaatggc gcaaatcttg tacattttaa cacaataaaa caaaaaatat
     3721 agaatatttc ctcataaatt tttccatttt ttgcaatatt ctcaaaaaaa agataaaaaa
     3781 tgagaataac tgatgaaaaa acaagcataa tccatcataa aatcagtata ggtgaaaaga
     3841 tgaactttca tctgcatatc aagtaattgg tatgaggcat tttttgaggt gtaggatatg
     3901 aagttggatt accttataga agaggaagat agagaaaaac tgctgctagc tagatattta
     3961 gaatttgaga aaaagaatta tgtagtgctg cctaagttat atgaaacatt aggtttgtct
     4021 aaatttaaaa caagaaatta tatcatagaa ttaaataaag aatttactga attcaaaaca
     4081 aagccagaga tccaaatttc agaagaagca gagatccatg tcgaacgtct gaatttgacg
     4141 gatatcaaaa atttaagaag aatctacttt caacgatcaa aaattgctcg actgttaacg
     4201 gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag agaatcattt tttaagcata
     4261 tcaagagcct atgtaaaaag aagagaatta gtctcttttc ttgaacagca tcaaataaag
     4321 ttaaagaaaa acatattagt tggggaagaa ataaacatac ggaacgtatt atttggggta
     4381 tatttcgggg tctttaatgg tttttctatg ccatttagag aaagcgtcat gaatgaagca
     4441 aagagtatcg tgcaatattt tgaatatctg ttcaatttaa aactgtctgc aacaaaacga
     4501 atcaaattca atttgctaac cactattagt ttgttgaggg cctatgggaa tcaaccaatc
     4561 ggaaaacgtt tcttttcaga tgaatacttt aagagctcag aaggaatgaa gatcatcaag
     4621 gatttatcac atatttcttt tcttcatatc cctaaagagc agatcattga tgagatttct
     4681 tatatcatgt tgtttttgat cacggaagaa gaatttgaat tagatggcgc atttggactt
     4741 gcggaagctc attttcatga tatccacatg attagtcaaa aaatgtcagt ggaattgatt
     4801 gatcaattac cattttcgat tgaaatctct gaagaagaaa gactatatat acgaaaagaa
     4861 cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga tattcgactt tgagatgact
     4921 agctttacgt ctaaaaagca gctacagttt ttcttcgaga cgtatccaat attcagtaca
     4981 gcaatccaaa aggtcataca agagaataaa cgctatttct tgggaaaaga tgaacgtttc
     5041 atgattcgct tattttatga ttatctcttt ttattggtcg gtatttgtcc ggtatcatat
     5101 tttgaagcac cgattcatgt ctgtattgat ttttcgcaag gatctgctta taccgattac
     5161 attatttctc aagtgaaagg atttaaaaat tacaatattg tgattgagag tcgaatcact
     5221 tctcgtacga atatttattt atctgattgt ttacttgaaa aatttaataa agaacagata
     5281 atttggaaaa atccaccgac tccagatgac tgggaatatt ttggaaatac catcatcaga
     5341 gaaaaaaata gacatttaac tgaaaaaatc agctagtatt tatggatggc agctttgtct
     5401 cacatattta ttagctgatt tatgtttttt tcaaagaaaa ggtaactaga tataactaga
     5461 tataactaga tatattactt aacaaaatta aaaagtacat aagttattat tccgattttc
     5521 ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag tccatcttct tttgatttta
     5581 tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag
     5641 gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt
     5701 ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg
     5761 ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc
     5821 atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat
     5881 actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc
     5941 agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg
     6001 gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt
     6061 gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt
     6121 ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa
     6181 atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac
     6241 gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat
     6301 acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa
     6361 acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa
     6421 gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca
     6481 aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact
     6541 acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg
     6601 gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact
     6661 cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta
     6721 aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat
     6781 ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac
     6841 ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg
     6901 aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat
     6961 cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag
     7021 ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt
     7081 acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg
     7141 attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta
     7201 catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagaca agaagaaagc
     7261 ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa
     7321 acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata
     7381 aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct
     7441 aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa
     7501 tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag
     7561 catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt
     7621 ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat
     7681 cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa
     7741 taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt
     7801 agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc
     7861 aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc
     7921 ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta
     7981 ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga
     8041 gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca
     8101 agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc
     8161 aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat
     8221 ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt
     8281 tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca
     8341 ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa
     8401 aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc ggataactaa
     8461 tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc
     8521 tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg
     8581 taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc
     8641 caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat
     8701 tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag
     8761 tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt
     8821 gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca
     8881 caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata
     8941 aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga
     9001 cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc
     9061 cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga
     9121 tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa
     9181 ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat
     9241 ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac
     9301 ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc
     9361 atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa
     9421 aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata
     9481 caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca
     9541 tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa
     9601 gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat
     9661 gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat
     9721 gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa
     9781 tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac
     9841 tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa
     9901 atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt
     9961 atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg
    10021 tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag
    10081 cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg
    10141 gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca
    10201 atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc
    10261 gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg
    10321 attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg
    10381 cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc
    10441 gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca
    10501 attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt
    10561 gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa
    10621 gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg
    10681 aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat
    10741 gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg
    10801 ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg
    10861 gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga
    10921 aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt
    10981 gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg
    11041 agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg
    11101 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt
    11161 tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg
    11221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacagc ggaagcagaa
    11281 gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
    11341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt
    11401 ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg
    11461 cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt
    11521 ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc
    11581 acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag
    11641 gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac
    11701 ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc
    11761 gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt
    11821 gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt
    11881 aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc
    11941 acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag
    12001 tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt
    12061 atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag
    12121 attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc
    12181 aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga
    12241 ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc
    12301 gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg
    12361 atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg
    12421 tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt
    12481 ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct
    12541 cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt
    12601 gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa
    12661 tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga
    12721 ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat
    12781 ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga
    12841 tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg
    12901 aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt
    12961 catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta
    13021 caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac
    13081 accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt
    13141 gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc
    13201 cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat
    13261 ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta
    13321 aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt
    13381 tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga
    13441 tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg
    13501 ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc
    13561 tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag
    13621 caatgattac aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta
    13681 aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt
    13741 ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga
    13801 cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta
    13861 aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac
    13921 gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg
    13981 ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta
    14041 gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta
    14101 gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc
    14161 gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa
    14221 tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag
    14281 cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat
    14341 tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc
    14401 tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa
    14461 aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt
    14521 tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag
    14581 ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga
    14641 gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc
    14701 ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg
    14761 agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag
    14821 ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga
    14881 gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt
    14941 ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc
    15001 acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg
    15061 tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt
    15121 tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc
    15181 gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga atcaattcca
    15241 atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca
    15301 gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc
    15361 tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt
    15421 gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat
    15481 gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag tttatgtaac
    15541 agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg
    15601 gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta
    15661 ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct
    15721 ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta
    15781 gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat
    15841 aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc
    15901 aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc
    15961 aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga
    16021 aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca
    16081 taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat
    16141 aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc aaaaaaaatt
    16201 ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt agtaaacagg
    16261 aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg agaagttaag
    16321 aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca cagatttgtc
    16381 aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg aaacattctt
    16441 ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag aagcagcgaa
    16501 tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag aaaacgggta
    16561 tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac
    16621 ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa cgtttatttt
    16681 tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg caaaagcagg
    16741 acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg
    16801 aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt aaaaacgtgg
    16861 ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa acagtattaa
    16921 aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt ccttccggct
    16981 gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca gaaggggaca
    17041 tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa gtaaatacag
    17101 tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt gcttttggat
    17161 tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta
    17221 tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt ggtcaaagac
    17281 agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta ttagatgaac
    17341 cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg cgggatctgc
    17401 aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa gcgttggcaa
    17461 tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt acacccgtag
    17521 atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag agcaatatcg
    17581 tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa tttgaatgtg
    17641 tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca gaggatttga
    17701 cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg
    17761 gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg gtccattcaa
    17821 cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa gatatccatg
    17881 tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc tacgaagaat
    17941 aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata ttttgtggct
    18001 cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt ttgatatgaa
    18061 tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat cgctttctat
    18121 gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga tcatcagtta
    18181 cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga tgctgattat
    18241 tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga ttttcagtgt
    18301 gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac agatattatt
    18361 cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta tgattatgcc
    18421 aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta gagatttagg
    18481 cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa atggtgtgaa
    18541 aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga ctcgtctgat
    18601 tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct tggttacaca
    18661 aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt tcatcgtgat
    18721 gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc
    18781 gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct atctgatttt
    18841 ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt gggaaaatta
    18901 tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt tcatgctggc
    18961 tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg gaatctatta
    19021 cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct tattagtctc
    19081 tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag gattcggatt
    19141 aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg tcgtcttgat
    19201 ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta gagaccttgg
    19261 tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc caggaatcat
    19321 agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta catttttcgt
    19381 cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa gacaaggaat
    19441 cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga tattggtcat
    19501 tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt
    19561 acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt aggtattctc
    19621 atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt
    19681 ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag tttgatcacg
    19741 aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc taatgaagcg
    19801 atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc aagtgaatat
    19861 atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc gaagatcaaa
    19921 ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga taatcagtat
    19981 tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt ctttgatgca
    20041 aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag tttgatgctg
    20101 attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta ttctttgaat
    20161 agtaagaata tggcacagct gacagaagca gcaaataaat tgaatcattt aacaccaaat
    20221 gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga agcctccgtg
    20281 gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca tttacattat
    20341 gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc aaaaaccgtg
    20401 aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga aaatgcggca
    20461 caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc gttattgcca
    20521 aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc ccatcttgaa
    20581 gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt cttagaattc
    20641 aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca acgttctaaa
    20701 gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa agcggatgaa
    20761 acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca gagaaaagtg
    20821 gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat tgaaaaagtt
    20881 acagaaggat aaagtaattg gaaactatgc aa
//
LOCUS       DAXWLB010000037        20865 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXWLB010000037 DAXWLB010000000
VERSION     DAXWLB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.037"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(15..1388)
                     /locus_tag="ACGE4H_001042"
     CDS             complement(15..1388)
                     /locus_tag="ACGE4H_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8364476.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(1388..1783)
                     /gene="rbsD"
                     /locus_tag="ACGE4H_001043"
     CDS             complement(1388..1783)
                     /gene="rbsD"
                     /locus_tag="ACGE4H_001043"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFF8364477.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(1811..2728)
                     /gene="rbsK"
                     /locus_tag="ACGE4H_001044"
     CDS             complement(1811..2728)
                     /gene="rbsK"
                     /locus_tag="ACGE4H_001044"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFF8364478.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(2836..3849)
                     /locus_tag="ACGE4H_001045"
     CDS             complement(2836..3849)
                     /locus_tag="ACGE4H_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFF8364479.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(3930..4529)
                     /locus_tag="ACGE4H_001046"
     CDS             complement(3930..4529)
                     /locus_tag="ACGE4H_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8364480.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            4715..5416
                     /locus_tag="ACGE4H_001047"
     CDS             4715..5416
                     /locus_tag="ACGE4H_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364481.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            5426..8137
                     /locus_tag="ACGE4H_001048"
     CDS             5426..8137
                     /locus_tag="ACGE4H_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFF8364482.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            8588..10216
                     /gene="treC"
                     /locus_tag="ACGE4H_001049"
     CDS             8588..10216
                     /gene="treC"
                     /locus_tag="ACGE4H_001049"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFF8364483.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(10276..11457)
                     /locus_tag="ACGE4H_001050"
     CDS             complement(10276..11457)
                     /locus_tag="ACGE4H_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8364484.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            11709..13232
                     /locus_tag="ACGE4H_001051"
     CDS             11709..13232
                     /locus_tag="ACGE4H_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFF8364485.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(13301..14028)
                     /locus_tag="ACGE4H_001052"
                     /pseudo
     CDS             complement(13301..14028)
                     /locus_tag="ACGE4H_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
     gene            complement(14041..15321)
                     /locus_tag="ACGE4H_001053"
     CDS             complement(14041..15321)
                     /locus_tag="ACGE4H_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8364486.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(15462..16064)
                     /locus_tag="ACGE4H_001054"
     CDS             complement(15462..16064)
                     /locus_tag="ACGE4H_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8364487.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            16309..16515
                     /locus_tag="ACGE4H_001055"
     CDS             16309..16515
                     /locus_tag="ACGE4H_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364488.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            16827..17546
                     /locus_tag="ACGE4H_001056"
     CDS             16827..17546
                     /locus_tag="ACGE4H_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8364489.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(17627..18310)
                     /locus_tag="ACGE4H_001057"
     CDS             complement(17627..18310)
                     /locus_tag="ACGE4H_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFF8364490.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            18528..20714
                     /locus_tag="ACGE4H_001058"
     CDS             18528..20714
                     /locus_tag="ACGE4H_001058"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFF8364491.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7121 a         3740 c         4071 g         5933 t
ORIGIN      
        1 gtctttttca ttaactattc ataagacatt aggtcttcat caaacccgct aacgagagta
       61 gtgatcattg ctacgactag actaacagcc agtcctgcta agtaccagaa aaaagtaccg
      121 gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa aagcgatcgc tttcacttgg
      181 gcaaatccca ttacagctcc accagcagct gcaccaatgc aagcagcaac aaatggtttt
      241 ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca ctcccgcaag taaaatcgaa
      301 gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt tacttcgcag aaatacacct
      361 aaagctgcac cagccattcc aaaattggct gctcctgtca acggaacgat tcggtcgaac
      421 ccataagcag agatattact gatcatgaca gggtttacag cccattggat accaaatatt
      481 accaaaatat tccaaccacc agcgatgatt gcgccactga tcggtccgtt aatatccatc
      541 atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa cacccgcagg cccgaatacc
      601 attaacgtca aaggaaccgt gataactaat gaaagtaaag gtacaaatac taattgcata
      661 ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag aataaaagaa aatacctaaa
      721 atcgccggca tgacagtaga cgtatactgc atcagtacta caggaatccc cagaaaatca
      781 gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat tcggttcaat cagtgctgca
      841 gcaatgacag ccgcaatata tccattcact ttaaacgttt tggcagcaga aaaagcaaga
      901 aagattggca gaaaataaaa gacggcattc gacgcagcag acaaaatatg ataagttccg
      961 cttgtttcac taagccagcc aatcgtagtc gctaaaacaa ccagaccttt taatatacca
     1021 gaaccggcaa acagcggaat gatcggcgta aagatttccg atatcaaacc aaatagtttt
     1081 tcgatcggat ttagcttttc tttcggagct gaatgatcga tttgacgcgt caattccttg
     1141 taagcaggag ccaatccctg atccatgatt atttctgcat atctatcgag taaaatgaaa
     1201 tacttaatgg aagggatttg ctgttttaat ttttctgcat tggctactgg ctgttttttc
     1261 aatttgatac gaagccgagt cgcacatgga aggacagaat caatattgtc ttttcctccc
     1321 aactgttcaa tcagcatatg aatctgctcg gacagtacca tctgttcagg attcattgtc
     1381 gtttccatta gaagaccact ccactcacta atataatatt ggcgtaaggt gtcatttcgc
     1441 ctgtacgaat aaatgcatga cagttattca actctttttt catttcttca tgtgggataa
     1501 agctaatcgg tgtatctggt aacaataatt tgattttttc caatatttcc gggttcattg
     1561 tctttatttc ttccgccaat tcaatcgatt gtacctcaag ttcttctaaa acgttttcta
     1621 aaacagataa aaaacttgga agaccgtttg agacagccaa atctattttt tcagtaccaa
     1681 aggggacagg catgcctgca tccccgatac ttaatttatc aaagtgcccc atttgtgcga
     1741 ttactcgtga aatatctgaa ttgattactt ttcctttttt catgtttgtt tcttcctctc
     1801 attgttaatt ttagactgct gattgttcta aagcttcgcg gtaagggata gaaggttgcg
     1861 caccataaga ctgcacagca acagaagagg ctagatttcc ataaaggatc gcttctttca
     1921 agttactaag gtctggctga agtgtgctgc ttaatgcgcc aataaatgta tcacctgcgg
     1981 ctgttgtgtc aacggcatcc actttaaaag cagggacgat accgtgttct ttttctgtat
     2041 gatagaaagc gccggcactg cctagtgtaa tgattactgt tccaatacct aattcatgga
     2101 gtttttcggc tgctgcgacc aaactgttat gatctgtcac tctgattcct gtaatgattt
     2161 ctgtttctgt ttcatttggt ataatgatat ctgtcttttc aagtagttca gtaggaatcg
     2221 tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc tgcgtctttt gcaatagaaa
     2281 aagctaggat cgtcgcatct aagctacttt caaactgagc aataataaag tcactgtttg
     2341 taatcagttg cttgtttttt aggacttctt gttcatggaa agcattattt gcccctgcat
     2401 ggatcaagat actattttct cctgctgcgt ctaccataat cattgcttgt ccggttgtgg
     2461 cgccttggat ttcagctatc ccagatgtat cgatgttttc ttttgtcagt agatctaata
     2521 actgttgtcc ttcgctgtca gccccaacgc ctccaataaa agatgtccgt gcgcctgaac
     2581 gtttggctgc cactgcttgg ttggcacctt tacctccgcc agcgtgaaaa atctcatgcg
     2641 catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg aagggtagtg tccatattaa
     2701 tacttccaat tactgtaacc gtgttcattt tacaaacctc caactatctc atttattttg
     2761 atgggaaaat tattttttaa aacgttttag taaaacgttt taatagttcg ataaaaatat
     2821 agcacacctt gaaagctatt tcaagggtgc agttgaaaat cttttttcta atcgtacagg
     2881 taatcttttt tcttcccatt ctttttctgg gaattggatc cgatctaaca ataacttggc
     2941 agatgtttgt cccaattcga agattggttg tgcgatcgtt gtcagtttcg gcttgatata
     3001 ctcgcacata tctatattat catagccaat aatgctgtag tcttcaggga tgctttttcc
     3061 agcttcctct aatccgcgat ataatccaaa agctaattca tcatttaaag caaacacacc
     3121 agttgcagca gaatcaagaa gttctgctgt gatctggtag ccaccttgtt ttgagaactg
     3181 tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa acatcaagag cagatttaaa
     3241 cccctcaatc cgagcgtgga cattttccgg tggattttca ggatagacaa gggcgatcgt
     3301 ttgatgtcct agactaagca agtgcatgcc cgctaggtaa ccaccgcgaa aatcgtctgt
     3361 tcgtacggca tcactaaatc cttcagattt tttctgatcc aaaacaatga agggccggcc
     3421 ttgttttttc aaattttcgt tgattgcatc tgtagagaca gcagaagtcg cgataatgaa
     3481 tccatccaca cctcgacgtg tgagctcggc tagatattcg atttcttttt gatgatcaga
     3541 atcagcatta cataatatcg tgacgaaatt ctgcttatac aagatatcct caattcctcg
     3601 catcagtgta ctgaagaatg gattcgtgat atcaggaacg agtacaccaa ttgttttcgt
     3661 ttccttggta atcatttgtc tagcaaaata gtcaggctga tagcctaatt cttctttggc
     3721 agctaaaact ttctgaacag ttttcggact gaatttctcc gtatttccat ttaatatcaa
     3781 ggagacagtt gtgattgaaa cgccggaaag tttagcgact tctttgattg tgatcttttt
     3841 tttcaccatg ttcatactcc tatctaaatc ctgtggttat tttcccatga aatcaaaaag
     3901 aagtaaatga aaatcacgtg tgccagtact tatttgcttt gttggatagc ttcgattatt
     3961 tgtttcgttg agcgtactcg tgcagttttc ggaaatatct tttttatgaa caattgatgg
     4021 gattctttgt cacgatcact gcaagcatct tcagcaagca cgatatggta cccgtgttga
     4081 aaggcatcta atgcagtagc atatacaccg tttgttgtcg ctacaccgct taaaatgata
     4141 gtttcgattc cacgccggcg aagttgaaga tcaagagaag taccaaaaaa agcacttgga
     4201 ttgtattttg tgatagtcaa catattatct gtatctttca aagaatcctt caaaagtaaa
     4261 tcagtgaatt ctggaggtac agaagcagtc attccaccca tatcagtttg ttgactcaaa
     4321 taagggaaag tggagatgtc tacgttcact aaaactttca aggcagcggt acgtttcaat
     4381 gttcttacta acaaatcatt gtttgtcaga atagatgctg cagtgtgtgg agagagtgta
     4441 ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca
     4501 aaaatcatat tttcctttaa atcattcatg atgttccttc tttcaaataa caatgttttt
     4561 tatgtaagta aatcataaat cgtttatttg tcaactaaat taaaggattc ctttctttag
     4621 tctagagaaa ttcagtggta aaataaataa gtgtgagaaa acgcattcga tgcatagata
     4681 tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag tatgtcgaag taaaaaatga
     4741 atacaaacga tacaaaatgg gagaaacgac cattacagca aatgacgata tttctttttc
     4801 aattgaaaaa ggcgaattag tgattatact tggtccaagt ggagcaggaa agtcaacgat
     4861 tcttaatatt ttaggtggga tggatacacc tgatgaaggt cagatcatta ttgatgacac
     4921 agatattgcg caattttcag ataaacagct tactgcttac cgacgaacag atgtagggtt
     4981 cgttttccaa ttctacaatc tcgttcctaa tctaacagca aaagaaaatg tggaattggc
     5041 aacggaagtt tctcctgatg cgcttgatcc tgtagaggta ttgaggcaag tgggcctagc
     5101 tcatcgattg aataactttc cgtcccaatt atcaggaggc gaacagcaga gggtttcaat
     5161 tgctcgtgca ctagctaaaa atcccaaact tcttttatgt gatgaaccga caggcgcgct
     5221 ggattttgaa acgggcaagc aagtattgaa attattgcaa aacgctagca gacaacacgg
     5281 caataccgtt ttgatcatta cacataattc tgctttagcg cccatcgctg atagagtcat
     5341 tcacattaat gatgctaaag taaggtctgt tgaattaaat gatcatcctt catccattga
     5401 tgagattgta tggtaggggg attttatgaa aacaagaaca tatcttaaag caggttttcg
     5461 cgaaattcgg caatctaaag gacgattcat tgcaatcatc cttattgtca tgctaggaac
     5521 acttttattt gtaggtgtca aaactgctgg acctgtcatg caaaaaacga tggatcacta
     5581 tgttggtaca gcaggacttt ctgatttgca aatcgtttct actggtggct tgacacaaaa
     5641 agatattgct caagcagaaa agatcccaga tgcaaaagta gaaacaggaa aacagatcta
     5701 ttattccaat actggcaaaa acgaagtgat tcaaatattt tcttataaca aaaataacaa
     5761 gcagaacaag ctacaattaa ctgatggaaa actcccggaa aaaccaaacc agcttgtatt
     5821 ggatgaaaaa gcggaagatg aagggtataa aatcggtgat atttatcaga ttgattcaga
     5881 tgaattgaaa gaaaaagagt ataccatcac gggctttgtc cgttctccat tgttcatcaa
     5941 caatttggag agaggatacg cgaacgtcgg aaatggtagc gtcgattatt ttgtctattt
     6001 acctgaatcc gtttttaaaa cagatattta ttctgtctta tatcttgatt ttacaaacgt
     6061 aaagaatttg aatacgtaca gcaaagctta caaagataaa atggagcaaa atcaagaaaa
     6121 ggcagaaaag tatttacaag atcgacccga agaacgctta gaagagctga aaaaatcggc
     6181 caacgaagaa ttagaaccgg cacagcaaaa aatagcggat ggcaaagcac aaacaacgca
     6241 agcaagaaca cggttagaag atgctaaaaa acagttagcg caacagtcag caactatcca
     6301 gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca ttatctcaac aagaagctca
     6361 acttgctgaa caagaaaggc aattagttga gaaagaacgt gaactggcct ctgcagagga
     6421 agaattgaat cgaaatgcag aaaaaattga aaatttaact aaaccatcat acctatacaa
     6481 tgaacgttca gaaaatgtcg gttttcaaga atttggtgat ctgtctgatc gtatcgctgc
     6541 gattgccaat gtttttcctg ttttcttttt ctttattgcc gcattgatca cctttacgac
     6601 aatgacacgt atggtagaag aaaaccgcag ggaaatcggg acattaaagg cgttaggtta
     6661 tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca agtctagcct cagggatagg
     6721 tattgtttta ggaacgatat taggaacgaa tcttttgcca aggatcattt ttgagctgtc
     6781 taacgagcgg tatgatattg ggagtgcgct tattttttat gactggccgc cgattttaca
     6841 agcagctgcc gcattcttca ttgccgcatt tggcgctgca atgatcgttt tattcaaaga
     6901 tttaagagaa cggcctgctg cattgcttca accgaaagca ccgaagccag ggaaacgcat
     6961 ttttcttgaa tacatcactc cgctgtggtc acgccttagc tttaatcaaa aaatcagtta
     7021 ccgaaatctc ttccgatata agtcaagaat gcttatggcg attatcggaa ttgctggatg
     7081 tgccgggctg atggttgcag gcgtgggatt gaaagattct ctaagttctg tttcagccaa
     7141 acaatttgga ccgatcattg actatcaagc aatcgttaca ttggattcat ccacggaaga
     7201 tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa aaagtaaatg agatcctagc
     7261 tattcatact caaaccgtag aattgagaaa aaaaggacat gcttcgcaaa gtctgacagt
     7321 gatcgtacca agtaaaacag ctgatttgga tcgttttatc catttaactg atcttgaaga
     7381 gaacccacgc tccttgccta aaacaggaat cgctattacg caaaaagtag cagaactatt
     7441 tgacttatct acaggagatc atatttcgct ttatgatgaa gatcaaaaag aatggaaagt
     7501 cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt atttatatgt cccctgaata
     7561 ttatgcttca gttactgatc aatcgatgat ggataatgca tttttgctgc gtacagataa
     7621 gatgacaaca aaagaagaag aaatgctttc cgagaaactg cttgccacag acgctgtgac
     7681 aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa gaatcaatgg cgaacttaga
     7741 ctcggttgtc ttaatctttg tcgtattatc aggactgctg gcttttatcg tcttgtataa
     7801 tctaactaat atcaatattt ccgagcgtgt acgcgaatta tctacaataa aagtccttgg
     7861 attttttgat aaagaagtca cgatgtacat tgttcgagaa aatatcatct ttactctttt
     7921 aggaatcatc ggaggatttg gtgtaggata tatattgact gacttcatct tgcaacaagc
     7981 gtctatggag aatgtcatct ttccattagt gatcacatgg tgggcttatg tcttatcagc
     8041 aggattaact atcgtattta cggttattgt gatgattgtt acccatttca aattaaagca
     8101 tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa cagaggttgt gaaatcagcg
     8161 gtttgacctg agtattagag aaacaaacgg caaaatagtt tttcatattt tgccgttttt
     8221 tgagctaata ccgaaggaca gctgattgaa caccatgaat cagaggttgt aaaaaagccg
     8281 tcctgcatcg agcaaaaaga atactcaaat aaaattagct cctcatgttt tacatttgag
     8341 aagactgatt gccgtagata aaagcttttg aacaccattt atagaagaaa caagaatatt
     8401 ttgctatttt aaacaagagg atagacaaat acatgtctga tctcttgttt tttattgggc
     8461 aacagacctc ttagaatgta taaagataaa ttttacaaac gctttcggtt cagttataat
     8521 aataaatgat aacttgtata tataagttgt ggtaggtaat aaatcagttg aatggaggta
     8581 aatatccatg acatttaaag acaaagtcat ttaccaaatc tatcccaaat cttacaaaga
     8641 cagtaatggt gatggaattg gagatttaaa aggaatcaaa gaaaaactcc cgtatctgaa
     8701 tgatctaggc atcgacatga tttggctaaa ccctatttat ccaagccctc aaaaagacaa
     8761 cggttatgat atctctgatt acacagccat cgatcctatc tttggtacga tggaggaatt
     8821 cgaacagcta atagccgaag caaagaaata ccggatagaa atcatgctcg atatggtgct
     8881 gaatcacgtt tcgacagaac atgaatggtt caaaaaagca ctagcagggg accagcatta
     8941 tcaggacttc ttcattttaa gagaagagcc aacggattgg gtatcaaaat ttggtggaag
     9001 tgcttggtcg ccatttggag atacggggaa atattatttg cacttatatg atcggacaca
     9061 agctgacttg aactggcgga accaagcagt aagagaggaa ctattcaaag ttgttcgttt
     9121 ttggatggaa aaaggggtaa aaggattccg tttcgatgtt atcaatgtta tcggtaaaga
     9181 cgaacaattg aaaaataata aagaaaatga tggcaaacct gaatatacgg acaaaccaat
     9241 cacccatacg tatctaaaag aattgaacca agcaacgttt gggacggatt ccactatcat
     9301 cacagtcggt gaaatgagct ctacgacgat cgaaaattgt atcttgtata cagaacctag
     9361 tagagaagaa ttatcaatgg tctttaattt ccatcatttg aaagtcgatt atgaaaatgg
     9421 gaataaatgg tctactcctc catttgattt tgcagaatta aagcgactat ttcatacatg
     9481 gggagaaaag atgagtgagg gtaacggctg gaatgctcta ttttttaata atcatgatca
     9541 gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat cgaaaacaag gagcagagat
     9601 gctagcaacc atgatccatt tgaaccgtgg gacgccctat atttatatgg gagaagaaat
     9661 cggcatgatc gacccagact attcatcgat ttccgactat attgacgtag aaagtttgaa
     9721 tgcttataag ttactccttg aacaaggatt cacagaaaaa gaagcgtttc gtcggatcaa
     9781 agcaaaatct cgcgataact ctcgaacacc catgcaatgg tcagacacag aacaagcagg
     9841 atttactaca gggaaacctt ggttaaaagt tggaggcaag ctagaagaga ttaatgtaga
     9901 aaaagaacta tcttctgaag attctatttt gtcctattac aagaaattga ttcggctgag
     9961 aaaaacatat ccaatcgttg cacaaggaga ttatcatgct tatgaagcaa atcatccgga
    10021 agtttatggc tttcttcgcc aatttgaagg acagcagctg cttgtattaa caaatttcta
    10081 tgataaagat acaaggatcg ccatcccaga aggattcaca gaagccaata ttcttatcga
    10141 taattattcc gatactgttc ttcatcaaag tctggagcta aaaccatacc aggcaattgc
    10201 tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc tacgagctat tctcgtaaga
    10261 tcgacgtttt gtgttttatt taaaataatc ttcttcagaa aatgaagagt tttcatactc
    10321 tcctttatcg ccataaaatt tattgtagcg ttgtttgaat ttacggaaaa taaaagtaac
    10381 gagtaatttg actaacgtat agataggaat accaaaaata acgcccacaa ttcccattag
    10441 atcacccatg acaagtaaaa cgattaagat cgtaatcgga tggatctgta aacgatcacc
    10501 cattactcga ggaatcacca agtcaccgtg gagaagttgt acggcaaacc agacaatgac
    10561 aaatttgaca gccataaaag tagagtcttg gaatgcaatg atcaaacctg gaaagaaggc
    10621 gataaacggc ccaatatatg gaatgataca taacaaccca gcagaaatag ctaaaacact
    10681 agcaaattct aatccgatca gtaagaaaca aacccagtag actgctccaa ggataaatga
    10741 agcaatgatt tgccccttta gaaatgaacc caattgttga ttagcaatct ttgtcaaatt
    10801 tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa actgactgat aaaatttttg
    10861 aggatctctc aaaaggaaaa aagcgacaat tggaccagta aataaagtaa taaaaatagc
    10921 tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca ctttcccaat aatttccaat
    10981 aaatccagct atatcagacg tcacgtcgtc aatagaagcg aaaaattgat taaatgaatc
    11041 ggcaaaaggt gttttctcca aaaattgtcg gagactctct tgaaaatctt gaaacaaatc
    11101 tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga aaaggaataa gccatatgct
    11161 tccaagcccc aataaaaata aaataatcac ataaacgata atgactgaca agttcctcga
    11221 aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga taatacaaga tcagtgcaaa
    11281 caataatggg ggcaagatag tagcgataat gattccgaca ggcttgaata taaacgagat
    11341 ctgatggaaa aaataaatga tcagcgcaag taagataact attcctaaaa catagtaaga
    11401 ggtttttcct cctaaaaagc ggatcaaacg attcgttgta tctgtttctt ttttcactct
    11461 ttttcctcct ttatctataa cttcattata gacaaaagcg ggtataaaag gaaaatgata
    11521 gaggttgatt gcaaaaatat cgtcaatcat tcatataaac tttctcatca attaaaataa
    11581 gaaaacgctt gacagttctt ttaaccatat gataaattaa atcctgttgc taaaacgtaa
    11641 ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg aaagaagatg agaagaggag
    11701 tgtcataaat gaaacgattg ataggtatat tggccatgct ggttttagca ggtatgttaa
    11761 ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga aaaattgagc gtgatgacga
    11821 ccttttatcc catgtatgat tttacaaaag caatcgtcgg ggatgaagga gaagtagagt
    11881 tattgattcc agcagggact gattcccacg attatgaacc ttctgctaaa gacatggcaa
    11941 agatccaaga tacagatatc tttgtttata atgatgaaaa catggaaaca tgggtccccg
    12001 caatccaaaa aacattacaa gaaggcaatg tccatacgat aaaagcgaca gaaggtatgc
    12061 ttttacttcc gggaagtgaa gaaggacatg atcatgacca tgaacacggg gaagaaggcc
    12121 atacacatga gctagaccca catgtatggc tagctccaag tttagcaatc aagcaggtag
    12181 caaacatacg tgatcagctg attgaagctt acccagaaaa acaggaagta tggacaaaaa
    12241 atgcagcagc ctataccgag aaacttcaag cactccatca gctttatcaa gaaaccttta
    12301 agcaagcaaa acaaagatct tttgtcacgc aacatgctgc attcaactat ctagcgttag
    12361 aatatggatt gaatcaagta tcgattgctg gtctttcttc tagtgaagaa ccgtctgcgg
    12421 cgagaattgc tgaactgaaa cactttgtta aagaacatgg aatcaactat atttattttg
    12481 aagaaaacgc caaagatagt attgccagaa cgttagcaaa cgaagcaggt gtatctttag
    12541 aagtattgaa tcctttggaa ggattgacga atgaacaaat agaaaatgga gaaaactatc
    12601 tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac gacagagaca gaaaacccgc
    12661 tagaagatac tttgaccccc aaaaaagaaa agagcgtcta caacggttat tttgaagacg
    12721 aggatgtaca agatcgctct ctttcagatt gggcaggaac ttggcaaaca gtagatacgt
    12781 atgtagaaga tgggactttt gatcctgttt ttgaatataa agaaaaattg aatcaggata
    12841 agacagcaca agaatataaa gaatattata cgaagggcta tcaaacagat attgcttcaa
    12901 tcaagatcag tgagaaagag atggcattta cgtttaagaa tggaacaacg aaacgttcaa
    12961 attatcgtta tgtagggaaa gaaatcttga cctacgaggc aggaaaccgt ggcgtaaggt
    13021 atctattcga agcagaagat aaggaaagtg gcgcttttag atatattcaa ttcagtgatc
    13081 acacgattgc accggctgac tccgaacatt ttcatttata tgcaggaaat gaaagtcaag
    13141 aagtattgct gaaagaaatg gagaattggc caacatttta tccagataca atggatggac
    13201 gtgaaattgc acaggaaatg cttttgcact gataggcaaa aaagaaccga agggtttgga
    13261 tcggctggtc atttagtcgg tccaaactat tcggttcttt ttatgaaatc aatttcttta
    13321 atccacggat agaggctggg atggtctcgc cgtttgccaa cgtgacttct ctggtttttt
    13381 attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac ggataaaatt
    13441 ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg catgagtcaa
    13501 atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga tgtctttgat
    13561 cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt ggatttggga
    13621 catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt ttaaatcata
    13681 aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga ttttcatcga
    13741 catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt cacggatcaa
    13801 tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta aaaagtaaat
    13861 accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat cagaggtaga
    13921 aatatggaca taactgtcta agttttcaat catgattcta tttttgatca ccgttgataa
    13981 aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat cattactcct
    14041 ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg atgtttcaag
    14101 gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa ggccttcgtt
    14161 ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg gtaatgtatt
    14221 tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta tggtcaattc
    14281 gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga taataccaag
    14341 catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag gaaaatgaag
    14401 ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta aactacggat
    14461 ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt tcatcatctg
    14521 gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt cgccttctga
    14581 aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt ctctcaagtc
    14641 ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt gttgctctaa
    14701 taactgatca gctaatttct tattagagat tttccaataa taaaccaaga tgatcaggcc
    14761 aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag gtgtttcacc
    14821 agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg tcccgatcgt
    14881 accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt ttaaacaaaa
    14941 agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca atgaatctcc
    15001 tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta agtgatctcc
    15061 taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac gcgaagtcga
    15121 aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa aaatccctaa
    15181 aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg caaacagata
    15241 tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca aatattgcaa
    15301 cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa attatagcac
    15361 aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc ataccaaagc
    15421 agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt gtttttttca
    15481 tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt agctatttct
    15541 aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa taacgtaatg
    15601 agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg tttctcgggt
    15661 tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa aaagcagctg
    15721 attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt catcaccaag
    15781 ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga gtcggaatgc
    15841 aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc tgtttccgcc
    15901 catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag attattcaat
    15961 aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga tttgtctccg
    16021 ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata cgtacctcca
    16081 ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa agtggttttg
    16141 acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc ttaccaaaaa
    16201 aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt ttcgagtgat
    16261 ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat ggaaaatgaa
    16321 caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga atcaccaata
    16381 aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca
    16441 gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg caaatcaagt
    16501 acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt gagtctcccg
    16561 tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt tatgaatatt
    16621 gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg gctgcgacaa
    16681 gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac ttttagagaa
    16741 gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa gtagtaaaat
    16801 gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt gcaggaacga
    16861 tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa acaaaacctt
    16921 tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat ccagaaaagt
    16981 atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc aaagatgctt
    17041 tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg ctgtttcatt
    17101 tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa ggtcttttag
    17161 aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat ctactggttt
    17221 atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc cgggaatatg
    17281 aacagctaga acaagatcct gaactctatt cctactataa agaactcaat cgacgatacg
    17341 aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg gacaaatatg
    17401 atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag atgcgggcaa
    17461 agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat aacccgaaag
    17521 caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca ctcgcagata
    17581 tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa atagtgaacc
    17641 gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg atcaaaaata
    17701 aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa acaaggacaa
    17761 tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga tcaaaatctg
    17821 ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt aaatttccag
    17881 ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc cactggatcg
    17941 atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg ggtgtcaaac
    18001 taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag aaatgttttt
    18061 ctttttttct aaaataatca attagatact ttgttagaag aatccctaag aaaaagaaaa
    18121 gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct tggatgatcg
    18181 atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata taagtataac
    18241 tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc tcgtgaaagg
    18301 aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat cttagcataa
    18361 acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc ccattttcat
    18421 ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa ataaccaact
    18481 tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg aatgaaaaag
    18541 aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa aagcttcaac
    18601 aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat ggcgaagcac
    18661 ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc tctttcttta
    18721 aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca gctatcacat
    18781 atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa acgcaaacaa
    18841 tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa cgcatactaa
    18901 tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat aatttcagcc
    18961 agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg atatacaatg
    19021 aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac ttttcaaagg
    19081 atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat aatctttata
    19141 tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg cagaatatga
    19201 acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc attgataaga
    19261 atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc caagaacaaa
    19321 aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa agcgctttgg
    19381 agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc attgatgaat
    19441 ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag gaaaacagga
    19501 tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga ggatcagagc
    19561 cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc ttattaacta
    19621 attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc tataataaaa
    19681 atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt atggaagcct
    19741 atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt cagacattta
    19801 ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct tcacaaacgc
    19861 agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa gcagatagtc
    19921 catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa attgttcatt
    19981 ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg ctccggcagc
    20041 tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct cctaaatatc
    20101 aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa tcgaaatctg
    20161 cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa gaaatctatt
    20221 cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac cagagtgatc
    20281 aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc caagccttat
    20341 cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct tacagacaag
    20401 gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt agttaccgaa
    20461 atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg acagaaaaga
    20521 aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa gaagaacgaa
    20581 gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt gtacctcaaa
    20641 tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg agtcgtgtga
    20701 aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa gaaagaagaa
    20761 ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt tcgaagaagc
    20821 tatttctgga acacagttta ttcggaaaag ggaggtgtga caaga
//
LOCUS       DAXWLB010000038        20861 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXWLB010000038 DAXWLB010000000
VERSION     DAXWLB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.038"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            107..1873
                     /locus_tag="ACGE4H_001059"
     CDS             107..1873
                     /locus_tag="ACGE4H_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFF8364492.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1888..2325
                     /locus_tag="ACGE4H_001060"
     CDS             1888..2325
                     /locus_tag="ACGE4H_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8364493.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2338..3489
                     /locus_tag="ACGE4H_001061"
     CDS             2338..3489
                     /locus_tag="ACGE4H_001061"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFF8364494.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(3551..4249)
                     /locus_tag="ACGE4H_001062"
     CDS             complement(3551..4249)
                     /locus_tag="ACGE4H_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFF8364495.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(4242..5594)
                     /locus_tag="ACGE4H_001063"
     CDS             complement(4242..5594)
                     /locus_tag="ACGE4H_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFF8364496.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(5817..7217)
                     /locus_tag="ACGE4H_001064"
     CDS             complement(5817..7217)
                     /locus_tag="ACGE4H_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFF8364497.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            7378..8115
                     /locus_tag="ACGE4H_001065"
     CDS             7378..8115
                     /locus_tag="ACGE4H_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFF8364498.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            8240..8719
                     /locus_tag="ACGE4H_001066"
     CDS             8240..8719
                     /locus_tag="ACGE4H_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFF8364499.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            8716..9483
                     /locus_tag="ACGE4H_001067"
     CDS             8716..9483
                     /locus_tag="ACGE4H_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFF8364500.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            9713..10282
                     /gene="lepB"
                     /locus_tag="ACGE4H_001068"
     CDS             9713..10282
                     /gene="lepB"
                     /locus_tag="ACGE4H_001068"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8364501.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(10386..10988)
                     /locus_tag="ACGE4H_001069"
     CDS             complement(10386..10988)
                     /locus_tag="ACGE4H_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFF8364502.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            11094..11324
                     /locus_tag="ACGE4H_001070"
     CDS             11094..11324
                     /locus_tag="ACGE4H_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364503.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            11315..11563
                     /locus_tag="ACGE4H_001071"
     CDS             11315..11563
                     /locus_tag="ACGE4H_001071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364504.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            11669..12253
                     /locus_tag="ACGE4H_001072"
     CDS             11669..12253
                     /locus_tag="ACGE4H_001072"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFF8364505.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            12256..13329
                     /gene="prfA"
                     /locus_tag="ACGE4H_001073"
     CDS             12256..13329
                     /gene="prfA"
                     /locus_tag="ACGE4H_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFF8364506.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            13322..14161
                     /gene="prmC"
                     /locus_tag="ACGE4H_001074"
     CDS             13322..14161
                     /gene="prmC"
                     /locus_tag="ACGE4H_001074"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFF8364507.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            14154..14333
                     /locus_tag="ACGE4H_001075"
     CDS             14154..14333
                     /locus_tag="ACGE4H_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364508.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            14355..15380
                     /locus_tag="ACGE4H_001076"
     CDS             14355..15380
                     /locus_tag="ACGE4H_001076"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFF8364509.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            15555..16799
                     /gene="glyA"
                     /locus_tag="ACGE4H_001077"
     CDS             15555..16799
                     /gene="glyA"
                     /locus_tag="ACGE4H_001077"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFF8364510.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            16952..17899
                     /locus_tag="ACGE4H_001078"
     CDS             16952..17899
                     /locus_tag="ACGE4H_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFF8364511.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            18033..18662
                     /gene="upp"
                     /locus_tag="ACGE4H_001079"
     CDS             18033..18662
                     /gene="upp"
                     /locus_tag="ACGE4H_001079"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFF8364512.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18801..19043)
                     /locus_tag="ACGE4H_001080"
     CDS             complement(18801..19043)
                     /locus_tag="ACGE4H_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364513.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            19164..19236
                     /locus_tag="ACGE4H_001081"
     tRNA            19164..19236
                     /locus_tag="ACGE4H_001081"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19197..19199,aa:Thr,seq:cgt)
     gene            complement(19355..19993)
                     /locus_tag="ACGE4H_001082"
     CDS             complement(19355..19993)
                     /locus_tag="ACGE4H_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFF8364514.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(20116..20748)
                     /locus_tag="ACGE4H_001083"
     CDS             complement(20116..20748)
                     /locus_tag="ACGE4H_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8364515.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         7095 a         3706 c         4202 g         5858 t
ORIGIN      
        1 gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag
       61 taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat
      121 tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag
      181 catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat
      241 cccagatggt tatttaccaa atgaggcttt cgctaccatg gtaagcccaa tgctaactta
      301 tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc
      361 agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct
      421 aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt
      481 ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat
      541 cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac
      601 tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag
      661 tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt
      721 gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga
      781 agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc
      841 agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat
      901 ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat
      961 gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg
     1021 gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg
     1081 cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc
     1141 gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc
     1201 ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat
     1261 ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa
     1321 agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga
     1381 tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc
     1441 accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat
     1501 cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc
     1561 agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga
     1621 tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca
     1681 tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact
     1741 gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca
     1801 tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa
     1861 acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc
     1921 aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa
     1981 gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct
     2041 gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg
     2101 aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa
     2161 gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa
     2221 cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac
     2281 gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg
     2341 agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca
     2401 gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat
     2461 gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa
     2521 aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa
     2581 gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt
     2641 gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc
     2701 tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg
     2761 gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc
     2821 ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc
     2881 attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat
     2941 gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc
     3001 gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta
     3061 gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg
     3121 gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac
     3181 aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat
     3241 caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat
     3301 ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt
     3361 caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga
     3421 atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa
     3481 gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa atttttgtca cattcccctt
     3541 tttcgaataa ctaggctgtt tgtccttcaa gatagggcag atccaattct ttaccgtaac
     3601 gttgttctaa agctaggcgg atcaaacgag ctgccaggtt ctcatttctt gcaaggatag
     3661 gtccatggaa ataagaaccg aagacatttc ggtaaatgac accttcagat tggtcttcac
     3721 cattatttcc ttgaccttgt accactttcc ccaaaggtcg ttcaccttca cctaagaaag
     3781 tccgaccatt gtggttttca aatccataat aagtttcatt gaattcttcg ttatggataa
     3841 cgatatcacc gataaaccgg ttattttcct gacttaacgt gtagtgatcc agtgcactga
     3901 ttccttggat cttttctcca ttagctccca tgtagtagtg acctagcaat tggtagccac
     3961 cacaaatggc taacatgacg ccatcatttt cgatatagtt gatcaaagaa tctttctttt
     4021 tctggatgtc ttttgaaatg attaattgct caaagtcttg accaccgcca aagaaaacca
     4081 agtcatattt atcagcatca aaaggttcat gtatactaac gatctccgta tggcaagaaa
     4141 cccccatttt ttctgctata tatttcaaca tcaaaaggtt tccgttatca ccatatgtgt
     4201 tcatcaaatt tccataaagg tgggcgatat tgagttcata attagccacg gttcattcct
     4261 cctttgataa atccttgagc agctagttct ttacgaagct gcaatacagc agtgtaagta
     4321 gctaaaatat agacatgctc agttggcaat tctttgattt tttcgattac ttctggtaat
     4381 tcttttgttt ctgtcaattt attttctgga atcccggcaa cttttagacg taaagtcata
     4441 tctgtatgac ggtctcctcc agaaataaca gctgggatat ccatatcggc aaagttttca
     4501 aaattaccat cccaaatcca gctgacatcg attccgtctg cgtaattggc atttaataaa
     4561 gccactaaag aaaatggata aggagctaga ccaatcatat cgattacttg attgatacct
     4621 acaggatttt tgacaagtac taatgtacat tttttcccat tgatattgat gacttcttgt
     4681 cggccgaata ctttctcgtc atatcctaaa ccttgtttga ttttatcagg atcgacattg
     4741 aagtactctg ccactgcagt agctgctaaa gcattgtaga cattatacat accgccaact
     4801 tcgattccgt attcatgacc atcgatcacg aaatctgctg acacattgtc catccgtacc
     4861 atatcagtta atcgataatc caattcagga cgtttgaagt cacaatttgg acagtaatat
     4921 ttccctaagt ttgcataagt gatcatttta taatgcaaga tgtgatgaca atttggacat
     4981 aagacaccgt ctgtattgta atgagccatt tgttcacgat cttcttcatg gtcaaagcca
     5041 taatattttc taggattgac tgtttcgata gaattgaaga ttggtgaatc accgttactg
     5101 ataatcgttg catttggtga ctttgcggca ccgtctacaa tcattttata ggtcgtatag
     5161 atttctccat accgatccat ttgatcacgg aaaatatttg taaataagaa cagttctggt
     5221 ttcatatatt ctgtgacttt gctcaaactt gcttcatcta tttctaggac agcaaacttt
     5281 ttctgtcctt tttttggttt tgcctgcaag aaagtcgata caattccctg taccatattc
     5341 gcaccagtag ggttggttag aacttcatcg aattgctggc gcaaaatatt gactgttaaa
     5401 gcggtagtca atgttttacc gtttgttccg gtgacaacga cgacttggta gtcttttgcc
     5461 aaactatcta gtatatgtgg atcgactttc agcgctagtt tacctggtaa acttgatccg
     5521 cctttaaaaa aagtttttaa tgcccattgc gacgtttttc caactgcaat cgcaaaatga
     5581 ctgcgtattc ccattatgta acctccaaaa acgatttgct gacgaataaa ttcaactaat
     5641 cataccatat gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa aaggttacgg
     5701 ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag cttgaaacaa
     5761 aatgattgtt tcaagctgct gtgattttat tattccctct tacctatatt cccattttaa
     5821 ccgataataa ttttctcgct tggatattca tagcctagat ctcgtgtttc ttttggcacg
     5881 aataacatca atgtatatag cattccgatc cgcccaataa acatcagtgt agcaatcgtg
     5941 attttgccca caacagacaa gtctccggta atccctaaag ataaaccagt tgttccaaac
     6001 gcagaagtta cttcgatgat gatcgagatc agcgtttgtt cttctgtagc agataaaaaa
     6061 acgatacaaa agaaacacat accaagagaa agcataaaga caacgactga ttttcgcaca
     6121 tcatcttgat cgattctccg tccaaaaata ttgatgttat cttcactttt gaggaaggaa
     6181 tatagataca aaccgatgat tgctacagta gttgttcgaa tcccgccgcc tacagaacta
     6241 ggactgcatc cgataaacat cagtaaagaa aaaatgatca gtgttgtgat ttgaaaatca
     6301 cccaaatcgt gaatctgcag accagcatta cgagtcgtaa tcgaataaaa catcgaattg
     6361 atccatttga cactcatatt ggaatcttgg aataaatggt ccttttccaa tagataaatc
     6421 aagacagttc ccgaaacaaa caagataaca aaagctaaca cagctaattt ggtaaacaag
     6481 gaaaaacgaa acggaagctt cgcattggaa cgtttgtata atagccattc ccggcactcc
     6541 atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag gatcaagaaa
     6601 aaatagtcat gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc agagttcgtg
     6661 acagccgata tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg atcaaaatag
     6721 cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa ccagattaaa
     6781 atagcaaaag tgatccgaat cagtcggacg ataccagaca aacgcggttg attcatatcc
     6841 gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat gacaaaagca
     6901 gtagaaatca tcatgatccc caaaccacca acttgaaata atatttccag cagtataata
     6961 ccgttatcat tgaacacaga attgatatca aaagtcgata gtccagtcac actcactgta
     7021 ctgattgcca tgaacagcat gtccacaaaa gaaacatggc tatctggttc gcgaaaaatc
     7081 ggcatataaa atagaacgag tgataagacc gtcatcaata tataataaaa aacgatgatt
     7141 tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag ccttcgaaag
     7201 aataaatcaa catgcacagt actatcctcc tgacaatagt cgtttctacc agtatacaag
     7261 gggaattagg aatactcaaa gaaattatca aacagataac aatacagctt tcgattaaaa
     7321 tagaaatggc ttataataag gaaaatccta gaaaaaagaa ggaggaggaa taacaaattg
     7381 attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta tgatacagtt
     7441 ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa agcctaagat attaatacat
     7501 acatgctgtg ctccttgcag tacgcacaca ttggaattca tgtctcaata tgcagagatc
     7561 acgttatttt tttcaaattc gaatattcat cctaaaagtg agtatttgcg aagattacat
     7621 gaacaaaaac gatttgtaga ccaatttaat gagaaaactg accatcatgt tgcgctaatc
     7681 gttgatgaat accgtccaaa tgaattcacc aaaatggttt taacaaacca tcttgaaaac
     7741 gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga tcttgttgcg
     7801 caaaaagcgc aagaactagg atacgattac tttggaagtg ccctgaccat ctcgccaaag
     7861 aaaaatgcag ctttgatcaa tcagatcgga atggatatcc aaaagattta cgggacacaa
     7921 tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca aatgtgcaaa
     7981 gagtatgata tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa gcagcaagga
     8041 tgcgatctga aagaaatcaa tcgggaagca aaagcttact taaaaacatc aaccgtagtt
     8101 tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa gaatttatgg
     8161 tagaaataag gtagactgta taatttgtcc gtagtaaagt aaggaaacaa gaataaattt
     8221 caatgaagga agagataaga tgcgaaaagt agcacctatt ttgaagccaa tcgctgtatt
     8281 aacaatctcg acgattgtcg cgactactac aggtattgtg aatagttcca gctaccgtgc
     8341 accaagttca agttctagta ctgtagaaga aacaacacca actacttcag tctcgacatc
     8401 tgaaccaaca acaacggaaa gtttgaatga aaccactacg acaagtacat gggacgatac
     8461 aacaactagt tcgtcgtatg atgagacata tacaactgac agctcaagcg aaacaagtaa
     8521 tacggtacca gatggcactc catcggattc aaccaatcca agtacaagtc cggatacaag
     8581 tgctagctcc gaaacgatac caagcagtac gactgtagat gataacacgc aaaatgatga
     8641 tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat acccaaacgt ataaccagca
     8701 agcaggtgat tctcaatgac atttttacta gaccttatca acggggtgca taaattcctt
     8761 ggctatttag acatcagtcc taaatatctt aaccgtggat atacgatttt aagtgtgatt
     8821 cccacgcttt atttattgag aattgtttat ggactttggc aaaaccaaaa ctatcttcaa
     8881 tttttcttat atggacttgc atttctagtg cttgtttatt ttactgtttt aaatgtattt
     8941 tattactttt tagataaaaa cacgaaagcc gatgtcactc agctttttgt gaagtattta
     9001 cctgatgaag cgttcaacat ccagacagaa gaatcgatag ataatggaaa ttcgatcgat
     9061 aaagtgaata cacaagaggt catggtttct tatgacgaag attatcaatt gaaattagcc
     9121 gagaatatgc ggtatttgat tggaaatggt gagatcaaaa caaatgaact cggtcgcatg
     9181 gatggatttc tggtcgatag aaatacattg tatccttatt actatgtcaa gaaaacagga
     9241 gagaaagagt atgttctaaa tattggacga agctactcgg acatggaaaa agtagggacc
     9301 attcgacttg actcaacaga agacacattg gaacctgtcg gattgttcat cgtcggcggt
     9361 gattttgtca aagacgggtt acgctatcat gagccttatc gtctgaaatt gattgcaaaa
     9421 aaggcacagt cgcaggaaga accagtggtt tatagtcgta gccaaagaag aagacattca
     9481 taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa acgtttattc ggaataaggg
     9541 ggatgtgaca cgtcttttgt cacgttcctt ttttatataa cgagaaagat aagacgaaca
     9601 catcacttat tctttccttt catttataaa cattgtagaa taaagaaagt acaacgaagt
     9661 attgtgaaaa aacgtaataa ataacgagga aaatacaagt aggaggtaag ctgtgaaaga
     9721 atttttgaaa aattggggga ttttgatcct tgtattagcg gctattttat tagctagagt
     9781 gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg gaccctacat taagtgatgg
     9841 acaacgattg atttcatcta agatctcaaa ctatgaacga atggatatta ttactacaaa
     9901 ggaaccgggg gatgaagaac ggatgattgt taaacggatc atcggtatgc ctggagatac
     9961 agtcaagatg gaaaatgacc agttgaccat taatggcaaa aagtatgatg aaccttatct
    10021 ggatgagttc aaaaaagaat tttcagaaga taagttacaa ggagaatatg cttatagctc
    10081 aggattccaa gcacaagcag aaagttcatc cacctttacg agtgactttg aatatactgt
    10141 gccaaaagga aagtatcttg ttctaggcga taaccgcttg atttcaaaag acagccgaat
    10201 gtttgggtta gtcgacaaag atatgatcca aggaaaagtt gttttccgtt attggcctct
    10261 ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg ttttttgtca
    10321 cagcttcttt ttccgaaaat tccttcttat ttcttggagt tgctcacttt ttttgcaatc
    10381 tcattttaag ctttttggct gatggataag aaaagcgaag cgtttttgtt cataaatagc
    10441 ttttccccac gatggaaggt atgactagta gcagagacaa agcccgtttt ttccaatcga
    10501 ttattcaatt cttcttggct aaagccatta tggactttcg gatgactaat ttgttcattc
    10561 ttgtcaaagt ctacgatgat caattgaccg ccaggagcta aaatttcata aagttttgta
    10621 agaatattct ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat gagattagct
    10681 tggacagcag gaaattcgac agaagcatca gcgtggatcg ttcggacatt tttcagatct
    10741 gctgcttgga tcttttcttc tgccattttc agcatagttt ctgaagcatc ggcaatgatc
    10801 agttctttga atctttccgc aagcggtaaa ctaacaagcc cagttccgcc tccgtaatcc
    10861 agcaggactt tttggtggct gtcgcgcggt agataagtag ttagttcttc gcgaataatc
    10921 ttggctaatg cttgacggtc aggggaatca taatggttag cgatggaatc aaaaatattt
    10981 tttgtcatca aatagctcct tacgtaaaac tttgaaattt taacttatta tagcacgtga
    11041 aaagaaagaa agcatgctat actcaattca aaaagggaga atctagaaag aatatgccga
    11101 tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata ttactagact
    11161 tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa tcaagtaaac
    11221 tacctttatg gtgtgaaaaa agaaaagaag aatatttagg ctttatcact ctttcacaga
    11281 caagtgaaga tactgccgaa atctattcta ttgtatggga ataaaaaaag actatcatca
    11341 ccaaggatta ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa aacattacaa
    11401 atatttgcaa gtgaagactg tagacgaggg gcactactct atatatgatc aaactatttg
    11461 tttctacgaa tcattaggat tttcaaggtt ggaagttttt ccaaatttat gggatgaatg
    11521 gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag atttaatact
    11581 aatctgtgct tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt ataataccat
    11641 aggaacaaac tggaaggtga aaaagagcat ggcacaacta ttttttaagt atggcgcaat
    11701 gaatagcggc aagacgatcg agatcctaaa agtcgcacat aattatgaag aacaaaataa
    11761 gccagttgtc ttaatgacaa gcggtattga tacaagagat ggtttaggcg ttgtttcaag
    11821 ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg tatttgacgt
    11881 aattcagaaa atggaaagca aaccctattg tgtgctgatc gatgaagcgc aattcttaaa
    11941 aaagaaacat gtcattgctt ttacgagaat tgtggacgaa ttggatattc cggttatggc
    12001 atttggtttg aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat atttattgct
    12061 ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca aaaaagcaat
    12121 catgaattta cattatattg atggaaaccc tgtttatgaa ggcgatcaag tgcaaattgg
    12181 cggaaatgaa gcttattacc ccgtttgtcg aaaacattac tttcacccat caatgattac
    12241 agaaggagaa taagcatgta cgatcaatta caagcaattg aagaccgtta tgaagagctt
    12301 ggagaacttt taagtgaccc agaagtgatt tctgatacaa agcgttttat gcagctttca
    12361 aaagaagaag caaacacgcg cgaaacagtc gaagtttatc gtcgttataa agaagtagta
    12421 caaggaatca ccgatacaga agaactgctt ggtgaaaaac tggatgatga aatggctgaa
    12481 ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag aagtcttaga agaaaaaatc
    12541 aagatcttgc ttttaccaaa agatccaaat gacgataaaa atatcatcat ggaaatccgt
    12601 ggagcagcag gcggagacga agcagcatta ttcgctggcg atcttttcaa tatgtaccaa
    12661 aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt taccggaatc
    12721 ggtggatata aagaagtgat catgatgatc accggagaca acgtatattc gaaattaaaa
    12781 tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca agggcgtatc
    12841 catacttcca ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga gatcgatatt
    12901 gaagacaaag atatccgtac agatatctat catgcttcag gtgctggcgg acagcacgtc
    12961 aataaaacag catctgctgt acgtttgact cacttaccaa cagggatcgt tgttgctatg
    13021 caagatgaac gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt gcgtgctcgt
    13081 gtgtacgata agatccagca agaagcacaa agcgagtatg atgccaatcg aaaatcagca
    13141 gtcggaactg gggatcgttc agaacggatc cggacgtata atttcccgca aaatcgtgtg
    13201 actgatcatc gtatcggatt aactatccaa aaattagatc agattttagc agggaaatta
    13261 gatgagatcg ttgatgcatt gatcatgtat gatcaaacat caaaactgga agctatgcaa
    13321 aatgggtaaa atgacctatc gagaagtcct gtcacgggct tcttcttttt tagaggatca
    13381 tgggaaagaa gcatatagta tccaattttt atttttggaa cgaaaacaat ggaaaaaact
    13441 tgattggctc cttcatatga acgaagagat ttcagaagaa gaacagcggt tgatcgaaac
    13501 cgatctgcga cttctgcttg cagatcatcc acctcagtat ttgctaggat atgcagattt
    13561 ttatgatcat cggttaaagg taactgaagc aacactgatc cctcgaccag aaacagagga
    13621 gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg ttagaagtga tagatattgg
    13681 gacagggact ggagcaattg ctattagttt gaaagcagca agaaaaaatt ggcatatatc
    13741 tgctgtggat ctttcagaag aagcattaga agtagcgaaa gaaaatgctc agaaagaagg
    13801 aacgaaaatc agtttttatc atggagatac attaggacca gtaatggatc agcaatttga
    13861 tgtgattatt tctaatccgc cgtacatcag tcggaatgaa tgggaactga tggatgaaag
    13921 tgtccggtca ttcgaaccta aaatggccct ttttgctgag aacgatggat tagctattta
    13981 tgaaaaaata gcagttgaag caccaagcgt actaaaatca aatggaaaga tttttttaga
    14041 aatcggattt cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat ttccagataa
    14101 aaaagtggcg ataaaaaagg atctttttgg caatgaacga atgatccaag tttatggttg
    14161 aagttgtgga tgaaaaattt tttatttgtt tttttattat aaaaaaacac ttatttgaca
    14221 aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat
    14281 ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg
    14341 gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg
    14401 aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg
    14461 ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag
    14521 ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact
    14581 ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat
    14641 ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat
    14701 ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg
    14761 tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc
    14821 atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag
    14881 aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg
    14941 cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc
    15001 ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg
    15061 atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa
    15121 ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag
    15181 atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt
    15241 ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag
    15301 tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac
    15361 aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg
    15421 ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag
    15481 agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa
    15541 aggagctgtt gtttgtggta gattacaaaa cgtttgaccc tgatctttgg gctgcgatcg
    15601 ccaaagaaga agaaagacaa gaacataatt tggagctgat tgcctctgaa aactttgtct
    15661 ccgaagcagt gatggctgca caaggaagta tcttgacaaa taaatacgca gaaggttatc
    15721 cagggcatcg ctattatggt gggtgtgaat ttgtcgatat cgttgaaaat ttagcgattg
    15781 accgtgcaaa agaattattc ggagcgaaat ttgcgaacgt ccagcctcac tccggctctc
    15841 aagccaacac ggctgcttat ctcgctttgg tagaaccagg ggacacgatc ttaggaatgg
    15901 atctttccgc aggcggacat ctaacacatg gatcaccagt caacttcagt ggcaaaacct
    15961 atcactttgt tgcttacggt gttgatccga caacagaagt tattgattat aatgtggtac
    16021 gtattttagc tagaaaacat caaccaaaat tgatcgtagc aggtgccagt gcttacggac
    16081 gaacgatcga ctttgcgaaa ttcagagaaa tcgcagatga agtaggcgca aaactgatgg
    16141 tagatatggc acatatcgca ggattagtag cagcaggact tcatcctaat ccagttcctt
    16201 atgcagatat cacaacgact accactcata aaacattgcg tggccctcga ggcggcatga
    16261 ttttgacaaa cgatgaagca ttagcaaaaa aaataaatag tgcggtattt cctggtattc
    16321 aaggcggccc tctagagcat gtgattgcgg gtaaagcagt agcattcaaa gaagcattag
    16381 atcctgcatt taaagaatat agtgaacaaa tcatcgccaa tgcaaaagca atggtcaaag
    16441 ttttcaatca agcaattgga accagagtca tttcaggggc gacagataat catctgatgt
    16501 tgatcgatgt tcgcgagtta ggtataaatg gaaaagaagc agagagcatt ttggacagtg
    16561 tgaatatcac tgtaaataaa aactcgattc cttttgaaac actaagtcca ttcaaaacaa
    16621 gcggtattcg aatcggcacg ccagcaatca ctactcgcgg gttcaaagaa gaagatgctg
    16681 taaaagtggc tgaactagtt gtcaaagcat tacaagcaaa agatgataac gctcaactag
    16741 acgaagtcaa aacaggtgta cgcgaattaa ctgaaaaatt tccgttgcat aaaaaataaa
    16801 aatttaaatt ccattagtaa acagaaaagt tcaatgttgg gcttttctgt ttattttttg
    16861 ggaaaacgga ttctttgaca atatcggtga tgctctctag tatatgaggt gtaattttag
    16921 tcttcacact aaatctcagg gaggaaaagt catgactgat cgtatctcgt tagaaaagga
    16981 agcagtagca ttcagtgatg caaatgcgcc tcatccgcgt atctatgaac tagcaccgga
    17041 agaaggaaga gttttactag aagaagtaca agaatcacct gtgaacaaat attccgtcga
    17101 tattgaagac ttgatggtgg atactggcga atggggaaaa ctcaatattc gtctcgtacg
    17161 accagaaggg aatatggata aattaccagt tattttttat attcatggag caggctgggt
    17221 ttttggcagt gctcaaaccc atgataaatt gatcagagaa cttgcggttc gtacgaattc
    17281 cgtcgttgtc tttccagaat atagtcgatc accagaagca aaatatccaa cagcaattga
    17341 acaaaattat gctgtgttgc aacagctgaa taaattagcc aagtcaaaaa atctggatct
    17401 cgagaatcta atcgttgcag gagattcagt aggtggtaac atggctacag tcatgacgat
    17461 cttaacaaaa gaacgaaagg gcctcccaat atccaagcaa ttgctgtatt atccagtcac
    17521 aaacgctgat tttgatacag attcctatca tgaattcgga gaaaactatt ttttgacaag
    17581 agaaggaatg atgtggtttt gggaccaata tacgacgaat gaagaagagc gtgctgaaat
    17641 cacagcttcg cctttgagag caaatataaa tgaactaaat gatcttcctt cagcaatgat
    17701 tttaacggga gaagcggatg tattaagaga tgaaggtgag gcttatgcgc gcaaacttcg
    17761 tgatgcaggg gttcctgtta cacaagtacg cttccaagga atcatccatg attttgtcat
    17821 ggtaaatgct atggatcaga caaaggcaac gcgtgcagca atggcattat ctacttcttg
    17881 gatcaagaaa aaagaataag ctttgcttgt ccgcaggaaa acttcttttc aaaaacggat
    17941 tttcttagga aaaatggcga gtttgactag tcaatcactt gattttgttt tacaattaaa
    18001 aagaaacgac agaacaaagg agagcttaaa aaatgggcaa atttcaagtg atcgatcatc
    18061 cattgattca acacaaacta actattattc gggacaaaaa ctgcggaaca aaggtttttc
    18121 gtgaagtagt cgatgaaatc gctatgttga tggcatatga agtatcacgg gatatgccat
    18181 tggaagacat cgtgatcgaa acaccgatca ctgaaacaac acaaaaaacg ttgtctggta
    18241 aaaaagtagc gattattcca atcttgcgcg caggtattgg aatggttgac ggtatcttgg
    18301 aattgattcc agctgctaaa gtgggacata tcggtcttta tcgagatgaa gaaacattgg
    18361 aaccacatga atatttcgtc aaacttccag aagacattga tgcacgtcag ttgtttgtag
    18421 tagatccaat gttggcaaca ggcggatcag caatcatggc aatcgatgca ttgaaagaac
    18481 gtggcgcgtc gaacattaaa tttgtttgtt tggtagcagc acctgaggga gtaaaagctt
    18541 tgcaagaagc gcatcctgat attgacatct atacagcttc tctagatgaa aaattgaatg
    18601 aacatggtta tatcgttcct ggactagggg atgcgggtga ccgtttgttt ggtacaaaat
    18661 aagtaaatac gaaaaagggt gtgacacaac ttttgtcaca tccttttttg gtcattttta
    18721 acgtttgatg cagaacagct ttttcacagc tttcgtttgc atataaaaaa accatcccaa
    18781 aaatgcagag atggtcaaat tcaaatcttt tttagttctt tgtagttaac gattactaca
    18841 cccttacttt gcgtcatcaa gttcgcattt tctgcagttt catcaatctc aacaacagcg
    18901 gaatttttca attgctttga aatcgttcca gtttgcgact tgccgtgaat tgtgaacatt
    18961 acgtgagtat tgatcttgaa tttttcacca atttccgatg gtacgatagt tgtatcaaat
    19021 tttccaaaag ttgccatttt cataaaacct gcctttcaca tcaattatac cacgttttat
    19081 gaaaaatatc atgaaattaa actaattttc catcggactc tttacaatca gccaaataga
    19141 gtatataatt tcttttggat aaggccagct tagctcagtt ggtagagcaa cgcactcgta
    19201 acgcgtaggt cgtaggttcg atccctatag ctggcattaa aagagaagac agatatatca
    19261 aggattttta atccattgat acatctgtct ttttgtttaa aggaaataaa gtatgctttc
    19321 ttagcaatag agcatgtatt atttctataa aaatttagga gaataaaacg actagcaata
    19381 tagctgtgaa atcaaatatc acatgaactg cccaacttgt aatgatcgag ttgcttttaa
    19441 tggcagcttg gttgaataac aatcttgcca ccccttgtat caaaaggatg tgtgctacag
    19501 tgtgtaaaat atttccatta tcataagtac tgtaatggat aaatccaaag atgattgctg
    19561 acaagaaact agcgacagga atgctgaact tactagaaaa aatagcgaga aaatacactg
    19621 agaacaattc ttctccaagt atcatgatag gcaaaataat aaataataac gggctgaaag
    19681 cctcatttat tggattgcct gttaaacccc atttcaaaat ttgttgcaaa aataccgaga
    19741 ctgcggcact gattactaca ttgataaaaa aatatttggc tatatttttg acaggagcga
    19801 caggtttcga gaacattttt ttcgtatctt ttttaccaaa aacaagtgta aatgctataa
    19861 atccagtgat agttaaagaa aaaatgctaa taatggatgg cgcaaatagc aaccctaaca
    19921 aaccgagtac aacgacaata gtgcccaata tccctttttt aagttctatt ttttttccta
    19981 aaatgttatt catgataata actcctacta ttcagttttt gaagatagag tcagtataca
    20041 tgaagagtta aaataggcaa tgttttttta tggatatttg tattgctaaa aatttatctt
    20101 atatattcat tttgattata ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc
    20161 ttgcaactga ttaagcgtac aattttttag atgttccgag cgtacttcaa tcatttcaaa
    20221 ggagttgcta cttaaatcga tattttttaa atcagtatca aaaaagctag cattttctaa
    20281 atcattagta gaaaacatta ttttagtgag ggaacagtct acaaaatctg cgcttgataa
    20341 gacagagtgc tcaaaggtac tttcttttat gtttgaacca gataaattta catagtccat
    20401 tttacaattc tcgaataacg tgttcccaat ataactttcc atcaatttag tacctgttaa
    20461 tttacaattg acaaatttca cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa
    20521 atcacagttt tcgaatatac agtcggtaat ctccattctt tgaaaatcat tggaagcaaa
    20581 aaatgcatac ttgaaaatag atttgtaaat aattggatgg cagattattt ggttttcgat
    20641 gacctctttt tgtgaaagga tattatacag ttcaaaatct tcgttttcat agatatgaaa
    20701 aaaggatcct ttattcaagc ttgcttcatt aactttagga aaaatcatat ttaattacct
    20761 ccattttcat aaagtcatat taacaaaagc taaaccaact tacattagca gaaaatgaga
    20821 taggttttcg cctttttcga taacgatagt gtcgctatct c
//
LOCUS       DAXWLB010000039        20502 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXWLB010000039 DAXWLB010000000
VERSION     DAXWLB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.039"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            214..498
                     /locus_tag="ACGE4H_001084"
     CDS             214..498
                     /locus_tag="ACGE4H_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFF8364516.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="ACGE4H_001085"
     CDS             complement(779..1198)
                     /locus_tag="ACGE4H_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFF8364517.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="ACGE4H_001086"
     CDS             complement(1200..1784)
                     /locus_tag="ACGE4H_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFF8364518.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACGE4H_001087"
     CDS             complement(1831..3306)
                     /locus_tag="ACGE4H_001087"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFF8364519.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACGE4H_001088"
     CDS             3487..3798
                     /locus_tag="ACGE4H_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364520.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3914..6250)
                     /locus_tag="ACGE4H_001089"
     CDS             complement(3914..6250)
                     /locus_tag="ACGE4H_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFF8364521.1"
                     /translation="MLQLKDIKKYYKVGEPTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6392..7060
                     /locus_tag="ACGE4H_001090"
     CDS             6392..7060
                     /locus_tag="ACGE4H_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8364522.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7063..8106
                     /locus_tag="ACGE4H_001091"
     CDS             7063..8106
                     /locus_tag="ACGE4H_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8364523.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8343..9224)
                     /locus_tag="ACGE4H_001092"
     CDS             complement(8343..9224)
                     /locus_tag="ACGE4H_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8364524.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9383..10111
                     /locus_tag="ACGE4H_001093"
     CDS             9383..10111
                     /locus_tag="ACGE4H_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFF8364525.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10267..10584
                     /locus_tag="ACGE4H_001094"
     CDS             10267..10584
                     /locus_tag="ACGE4H_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFF8364526.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10577..11185
                     /locus_tag="ACGE4H_001095"
     CDS             10577..11185
                     /locus_tag="ACGE4H_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFF8364527.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11240..12031
                     /locus_tag="ACGE4H_001096"
     CDS             11240..12031
                     /locus_tag="ACGE4H_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFF8364528.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12169..12657
                     /locus_tag="ACGE4H_001097"
     CDS             12169..12657
                     /locus_tag="ACGE4H_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFF8364529.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12708..13580
                     /locus_tag="ACGE4H_001098"
     CDS             12708..13580
                     /locus_tag="ACGE4H_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8364530.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13756..14118
                     /locus_tag="ACGE4H_001099"
     CDS             13756..14118
                     /locus_tag="ACGE4H_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8364531.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14127..14879
                     /locus_tag="ACGE4H_001100"
     CDS             14127..14879
                     /locus_tag="ACGE4H_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364532.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14893..16695
                     /locus_tag="ACGE4H_001101"
     CDS             14893..16695
                     /locus_tag="ACGE4H_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364533.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16972..18012
                     /locus_tag="ACGE4H_001102"
     CDS             16972..18012
                     /locus_tag="ACGE4H_001102"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8364534.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18064..20208)
                     /locus_tag="ACGE4H_001103"
     CDS             complement(18064..20208)
                     /locus_tag="ACGE4H_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8364535.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6294 a         3592 c         4239 g         6377 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaaacagc gcgaaacaag gaacatatta
     3841 cttcctcgtt tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt
     3901 ggttgtcaca accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc
     3961 gtgcaggaag atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg
     4021 catggacagg atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat
     4081 tgatagggaa cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta
     4141 aaatacacgt ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt
     4201 ttagtacacc gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca
     4261 tgatcatact tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat
     4321 ccatcaatcc gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat
     4381 cttcatctga tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt
     4441 caaaattgtt cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa
     4501 gcaatgtttg tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt
     4561 ctctttgagc tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt
     4621 gatcgctata agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta
     4681 ggatccccga gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat
     4741 tagggataaa attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga
     4801 atgttgtacc aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa
     4861 gattcttcaa tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg
     4921 ttgctgaggc tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt
     4981 tgctagtgtc aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg
     5041 tactagataa agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta
     5101 ctttgccatt tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat
     5161 tacttaagtt aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg
     5221 tgtgctgtgc tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat
     5281 ccgctttgct tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa
     5341 tagtaatcgg aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc
     5401 cagaagaaag ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg
     5461 tcaagaaagt acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc
     5521 aaaaactcat ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag
     5581 gatgggaatc tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat
     5641 gggccagttc aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga
     5701 tcaattccat gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca
     5761 aaaggatatc tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac
     5821 cagataactg atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat
     5881 cttctgcccg ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa
     5941 cgttttcaat aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag
     6001 agttgtttcg gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga
     6061 ttaccatatc accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt
     6121 taccagaacc actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca
     6181 caccatccaa tgctttggtg gtgggttcgc cgactttata atattttttt atatctttta
     6241 attgaagcat aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca
     6301 gaacaatgat cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc
     6361 tagaaatttt tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga
     6421 tccgcaatta cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac
     6481 cgcattgaat ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat
     6541 tacagatatc atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac
     6601 cagatttgac acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg
     6661 cgggttcaaa ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat
     6721 cctacgtgtg gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat
     6781 cggacaaaca aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat
     6841 tctgcctcaa aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt
     6901 cactcgccaa cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac
     6961 gatcgatgta catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat
     7021 catcacagta cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt
     7081 tcacaattat ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg
     7141 tgttttctcg gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct
     7201 ggaaaaccaa cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca
     7261 ggaatatcga tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat
     7321 atgagtctgg tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca
     7381 gaagtacagc aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag
     7441 acactgagga atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca
     7501 atccaaggat atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata
     7561 tttttatatc ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta
     7621 aaattgaaca aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat
     7681 gaacaaatca gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa
     7741 ttggatatcg aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg
     7801 tggctgaata tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc
     7861 aaactatttg agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg
     7921 aatgaagaga ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa
     7981 atttctggga atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga
     8041 cgtattgatt atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa
     8101 caataggaaa aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt
     8161 cgtttttttg tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt
     8221 cttattgaac tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag
     8281 agagcgaaag tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg
     8341 gcttagtttt cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca
     8401 ccgactttac gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca
     8461 gtattttctt ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca
     8521 tttgcaatca tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca
     8581 aatccagctt cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg
     8641 agcaaaaagt tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga
     8701 tgatttttag gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa
     8761 cttggtgggg cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca
     8821 ttgtaagtta attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt
     8881 ttcaaaaact cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag
     8941 atatttaatg aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg
     9001 tattgttcta cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg
     9061 atttttctat gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc
     9121 tgtttagaaa tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt
     9181 gtttcggcta aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt
     9241 attccaataa agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta
     9301 caatctttct tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt
     9361 agaaaaagag aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt
     9421 gtttggcgga ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca
     9481 gctgacgatg gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat
     9541 gaaaaatatg ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt
     9601 tcatttaggt aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat
     9661 cttccctagt gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt
     9721 cattgttcta ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac
     9781 aggagacagt acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt
     9841 tgcgtgtaat ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta
     9901 tattttgttc ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga
     9961 ttttaaagct tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa
    10021 aatgttccca gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat
    10081 gtggacaaat tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct
    10141 gactaaaaag gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa
    10201 ctatattata tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt
    10261 gacgtaatgc acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta
    10321 agcaaggaag atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc
    10381 agtgaatcta cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc
    10441 tatgatgaac cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt
    10501 cgtcatttag aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta
    10561 ttggaggaga gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat
    10621 tcctgaagac gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg
    10681 aaaaacatta gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt
    10741 aaaaatcagt tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa
    10801 aagacaaatt cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat
    10861 tccactagca ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct
    10921 catttttagt atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat
    10981 ctcaggaatc agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg
    11041 tctgttcgcc acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg
    11101 gcttttttat gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag
    11161 agatctgcat tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt
    11221 gattaccggt attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt
    11281 aggcgctcaa aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga
    11341 gacaaaaaaa atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc
    11401 ggtcatacgc ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga
    11461 aataaaggag aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat
    11521 cattagttta gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt
    11581 atctgaattg acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa
    11641 aaaagtgaat actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac
    11701 aataactgcg aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt
    11761 gatcgtcaca ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg
    11821 aaaagtcatc ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac
    11881 tatccaaacg tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt
    11941 gttagaacaa ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac
    12001 gacggatcaa gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa
    12061 aaacagcaaa gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag
    12121 taaaataaga aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa
    12181 gaaggtcaaa gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa
    12241 ctaagccaaa ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat
    12301 tcagaaggtc gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg
    12361 aagaaagagc tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat
    12421 gggaagaagg aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg
    12481 atggatctag aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca
    12541 aaattggatt atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact
    12601 gaagacaaag agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa
    12661 agtaacatta gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa
    12721 gagcatttta tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg
    12781 gattgttttt cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta
    12841 tttttctttg gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc
    12901 aagcaaaagt aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt
    12961 tccttacgat acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata
    13021 gttctgtttt aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag
    13081 tctttaaagt cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg
    13141 tagaaatcca aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat
    13201 tcaatgagaa tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa
    13261 aagagctgca agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc
    13321 atttagccta tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca
    13381 ttttgtctgc tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag
    13441 agcaaatcga agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca
    13501 ataatttact tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg
    13561 acatgaaaga ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga
    13621 ttattgtctt gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa
    13681 tgatagttga ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag
    13741 agagaagggg aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta
    13801 cttgcagggg tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatgtacaa
    13861 attacgacag atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac
    13921 tatagatata tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag
    13981 ggacaattga ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat
    14041 aaaggtgtca cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa
    14101 aaactaatta aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa
    14161 aagtatacgg aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg
    14221 tagaaaaagg cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt
    14281 tgaatatttt atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag
    14341 atgtgaccac attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta
    14401 ttttccaaga tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc
    14461 tttctttaca aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac
    14521 gtttatccat ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac
    14581 aacgggtcgc cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc
    14641 ctacaggtgc gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga
    14701 atacaaaaga tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt
    14761 gccaacgtat tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc
    14821 aaagccgtga ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg
    14881 gggagattga ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac
    14941 tacttggtgt tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca
    15001 atggcaagca acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt
    15061 ttccgattcg gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat
    15121 tcctttttga tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa
    15181 ggcagaaaaa tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact
    15241 ttagtgggtt cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat
    15301 cagttagatc tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact
    15361 gttttgtttt ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc
    15421 aaaaaaccga ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat
    15481 aaattcttgt ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat
    15541 atgatgaaac acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc
    15601 gtattaggaa cttatttcat tttccattct gtgatcattt tctttttatc attactaaag
    15661 aaatcagaac agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt
    15721 cgtattcgtg aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg
    15781 ggcgctttga cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat
    15841 tctggatatg atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa
    15901 ctttcaccaa cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat
    15961 aatgcaagtg aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg
    16021 ccgaagatca cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag
    16081 gaacttcgaa gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa
    16141 tcagaatttg atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac
    16201 ttccaagcaa acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca
    16261 tctttgcaaa aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt
    16321 ctattctctg gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt
    16381 gctagctgtt tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa
    16441 atgcttgaaa agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta
    16501 ggcgtgttat tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa
    16561 atgtttggtc tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc
    16621 ttcgtattgt atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg
    16681 aaaaagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata
    16741 gctcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    16801 tttgttcgga aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt
    16861 tattagaata tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt
    16921 tccatgtata ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc
    16981 aatgaacaaa attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca
    17041 tctttgtctg tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat
    17101 gaaaaacgag agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct
    17161 tatatcggtg cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta
    17221 gatgatgaaa cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt
    17281 gcacctagct ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca
    17341 tcggtcattt tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat
    17401 gtagaagtac ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc
    17461 agtgaccgca aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat
    17521 ccagcacagt caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc
    17581 aataaagata caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc
    17641 caacgtccta aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg
    17701 ggcggcatcg atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa
    17761 ttgacaagcc cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg
    17821 atttttacag caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat
    17881 catggcggta tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt
    17941 ggagatgtca ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat
    18001 ataaagaaat agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt
    18061 attctatttc tttaaaggca gatcaagcca gaatgatttg tctaaactta acaaggttag
    18121 tacaggaaca gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt
    18181 aaaattgttc cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac
    18241 gcgttttatc gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat
    18301 tggcacatcg aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc
    18361 tgcttctttt ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa
    18421 tttttgtgaa tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt
    18481 gtagtctttt gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc
    18541 atttttgatc aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa
    18601 ttgtgcgcgg atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac
    18661 gctgcttcct ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa
    18721 taagctgttt ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat
    18781 tccttctttt tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt
    18841 tgtttggttt tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc
    18901 tgtcagcgaa cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg
    18961 cgtgaaatct gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa
    19021 ttgtccgttc ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa
    19081 gacgaggtcg ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc
    19141 gcctgtccag ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc
    19201 agaacagtca aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt
    19261 gaatgcttct cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc
    19321 aattggtgat tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg
    19381 atttgctgta tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt
    19441 tggatggttt ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg
    19501 attcgaagaa ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt
    19561 attattgttg ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg
    19621 gacttcttca ctaggatcag ttggtttctc tgttgaagta tccggtccag ctttaaattc
    19681 tgcagtaata gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc
    19741 tttggcacca ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta
    19801 agttaccttc gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg
    19861 ttgctgttca ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg
    19921 agcaattgta agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc
    19981 aatttcagtt ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt
    20041 aaaagaatcc gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt
    20101 ttgatcagct atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc
    20161 aatcagccaa gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc
    20221 cttttccttt cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga
    20281 taatcgtgtc gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa
    20341 tttgtaaata agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga
    20401 ccacctttgt ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata
    20461 ccaaagtttg gaaagtatca caaaaattta taaaaataat ga
//
LOCUS       DAXWLB010000040        20302 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXWLB010000040 DAXWLB010000000
VERSION     DAXWLB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.040"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(140..2215)
                     /gene="glyS"
                     /locus_tag="ACGE4H_001104"
     CDS             complement(140..2215)
                     /gene="glyS"
                     /locus_tag="ACGE4H_001104"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFF8364536.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2217..3134)
                     /gene="glyQ"
                     /locus_tag="ACGE4H_001105"
     CDS             complement(2217..3134)
                     /gene="glyQ"
                     /locus_tag="ACGE4H_001105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFF8364537.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3518..4330)
                     /gene="recO"
                     /locus_tag="ACGE4H_001106"
     CDS             complement(3518..4330)
                     /gene="recO"
                     /locus_tag="ACGE4H_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFF8364538.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4473..5372)
                     /gene="era"
                     /locus_tag="ACGE4H_001107"
     CDS             complement(4473..5372)
                     /gene="era"
                     /locus_tag="ACGE4H_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFF8364539.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5387..5782)
                     /locus_tag="ACGE4H_001108"
     CDS             complement(5387..5782)
                     /locus_tag="ACGE4H_001108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8364540.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5766..6242)
                     /gene="ybeY"
                     /locus_tag="ACGE4H_001109"
     CDS             complement(5766..6242)
                     /gene="ybeY"
                     /locus_tag="ACGE4H_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFF8364541.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6259..8451)
                     /locus_tag="ACGE4H_001110"
     CDS             complement(6259..8451)
                     /locus_tag="ACGE4H_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFF8364542.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8475..9446)
                     /locus_tag="ACGE4H_001111"
     CDS             complement(8475..9446)
                     /locus_tag="ACGE4H_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFF8364543.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10152..11531
                     /gene="brnQ"
                     /locus_tag="ACGE4H_001112"
     CDS             10152..11531
                     /gene="brnQ"
                     /locus_tag="ACGE4H_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8364544.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11685..12131)
                     /locus_tag="ACGE4H_001113"
     CDS             complement(11685..12131)
                     /locus_tag="ACGE4H_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFF8364545.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12158..12334)
                     /gene="rpsU"
                     /locus_tag="ACGE4H_001114"
     CDS             complement(12158..12334)
                     /gene="rpsU"
                     /locus_tag="ACGE4H_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFF8364546.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12496..12924)
                     /locus_tag="ACGE4H_001115"
     CDS             complement(12496..12924)
                     /locus_tag="ACGE4H_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8364547.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13016..13876
                     /locus_tag="ACGE4H_001116"
     CDS             13016..13876
                     /locus_tag="ACGE4H_001116"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFF8364548.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            complement(13968..14318)
                     /locus_tag="ACGE4H_001117"
     CDS             complement(13968..14318)
                     /locus_tag="ACGE4H_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFF8364549.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14333..16060)
                     /locus_tag="ACGE4H_001118"
     CDS             complement(14333..16060)
                     /locus_tag="ACGE4H_001118"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFF8364550.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(16274..17200)
                     /gene="trxB"
                     /locus_tag="ACGE4H_001119"
     CDS             complement(16274..17200)
                     /gene="trxB"
                     /locus_tag="ACGE4H_001119"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFF8364551.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17345..18778)
                     /locus_tag="ACGE4H_001120"
     CDS             complement(17345..18778)
                     /locus_tag="ACGE4H_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8364552.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            complement(18778..20181)
                     /locus_tag="ACGE4H_001121"
     CDS             complement(18778..20181)
                     /locus_tag="ACGE4H_001121"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFF8364553.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         5516 a         4369 c         3600 g         6817 t
ORIGIN      
        1 tgtttttaaa agttacacaa aaaataaaat aacaaaaaaa ttaacattga attaataata
       61 gttatttata tttataacaa ataaaccaga gaaatagcct attttgtgcc tgttctctgg
      121 tttatttaca tattagaaat tattttgtga tgatttgatc taaacttgct agtgacaaag
      181 ccatggcagc aatttgactt aattgcgtta atcggttctt acgtactgct ggttcttctg
      241 ccatgaccat cgtctgatca aaataatttt cgatcagtgg gcgaaggctt acaagctgat
      301 cataattttc gttgatcgtt tggtcagcaa agcttgtttt cattgcctct actgctttgt
      361 gcagttcttt ctccgcttcg ttttcgaaca gttcagggtc tacttctgat tgtttttcaa
      421 gctccgcttt tttcgctaag ttcacgacac gtgtcaaagc ttccatcgat ggtttgaagt
      481 cttcatctgc aagatgctgc ttgaacaatt gtgctgctgc aaatactttt gtcagatctt
      541 tttgttctgc attcaaaaca gcttcgatga catcatgacg gatattcttc gttgtcaata
      601 attggcgcag acggcctttg ataaagtcga tcacctgttg ttgtcccgct gacaagccaa
      661 tgccaaaacg atccgtatct ttactgataa cttcactaat cgtttcttgc agaacagaca
      721 aaggaaaggc ccattttttc gattcgatga ttcgaacgac accatatgct tgacggcgca
      781 aagcatatgg atcgtttgag ccacttggga tcatcccaac tgcaaagaaa gaaaagatac
      841 tatctaattt atcagcaatc gctaaaacag aacctactgt agtctcaggt aaatctcctt
      901 cactggatat aggcatgtaa tgttcacgga tcgctgttgc tacggctggt ttctcgcctt
      961 taagcagagc atatttttcg cccatgatcc cttgtagttc agggaattct cctaccatgt
     1021 tggttactag atcaaattta tagatttcag cagcgcgttg cagatccatc aattcttcct
     1081 cagatagtcc tacttgttcg cctatgatgc gagcgatgac ttgtacccgt tgcattttct
     1141 catagatgct accgattttt tcatggaaag taacagtttt taatttttca acgtattggt
     1201 cgattgaagc tttcttgtct tcattataaa agaattccgc atcttctaaa cgagcagtca
     1261 atactttttg attgcctcgt accacatttt ccaattttac ccgatcgcca ttacgagcag
     1321 aaataaaatg aggaagcaac ataccttgat cgttccgtac ttcgaaataa cgttgatgtt
     1381 ctttcataga agtgactagt acttcttcag gaacagatag atatttttct tcgaagtcgc
     1441 cagaaaagac agttggatat tcgactaaat tgttgacttc ttctagtaaa tcttcatcta
     1501 gatcaatgat ccagtgattt ttttctgcta attcgtttgc ttgtgtgatg atcatttctt
     1561 ttcttttttg cggtgaagca ataacaaatt gttcattcaa tttttcttcg tattgttttg
     1621 catgcgtaaa tgtcacatct tcgcctaaga aacgatgacc tcttgaagct ctgtctgttt
     1681 tgacatccaa gatctcaaac gggattattt cgtcatctaa caatgccacg atccagtgaa
     1741 tagggcgaat atattcaaaa tcgtattctg cccagtgcat cgttactggg aaagtgaggc
     1801 ttttaatgac atctgctaaa cctgtcaata cttcttttgc ttctttgccg tgagtgtatt
     1861 tcgttacata gacatattcc acgcctttta attctctaaa agtgatagca tctgtcgtca
     1921 gaccttgtcc gcggacaaat ccttcagctg ctttcgtcca gtttccatct gcgtccaaag
     1981 caattttttt ggctggtcct ttgacttctt cttcgctgtc tgcttgacgt tctggaatgt
     2041 ttgttacttt gattgctaag cggcgcggtg tagaaaacgt ttcgatcgta tcataagtca
     2101 attggttttc atctaaaaat tttgctgttt tttcttctaa ttgtttcatg cttggtgtga
     2161 ctacatgagc aggtatttct tccagcccga tttcaagtaa taagtctttt gccatcttat
     2221 tcagcctcct tcacgttttc tggatttgct gtgtttttat tcaataaagg gaatcctaat
     2281 ttttcgcgtt ctgccacaaa gattttggct acagcacgag ccatgttgcg gatacgggaa
     2341 agataacctg cacgctctgt tacggatact gcaccgcgag catctaataa attgaatgta
     2401 tggctacatt tcaaaatata atcatacgct ggatgaacaa gattttcatc gatacagcgt
     2461 ttagcttctt tttcaaaacg atcaaaattg cttagtagca ggtcttggtc gcttacttca
     2521 aaagaatatt tagaatgttc atattctggt tgtttgaaga tctcaccata tttcacgcca
     2581 tcagcccatt ccagatcata aacactttca acttcttgga tataagaagc aagacgttct
     2641 agtccataag tgatctcaga agtaactggt ttacaaggta gtcctccgac ttgctggaag
     2701 tatgtgaatt gtgtgatctc catcccatca agccatactt cccagcccac accggcacaa
     2761 cccatcgatg ggttctccca gttgtcttca acaaagcgaa tatcgtgttc taatggatca
     2821 attcctaaaa gacgcaagct ttctaaataa agctcttgga tattttcagg tgaaggtttc
     2881 attacgactt gaaattgatg atgctgatac aaacggtttg ggttttctcc ataacgcccg
     2941 tcagctggtc ggcgggatgg ctctacataa gcagcattcc atggttccgg tccgattgcc
     3001 cgtaagaatg tatatgggct catcgtacct gctccttttt ctgtatcata agcttgcatc
     3061 agcatgcagc cgtttgatga ccaaaatttt tgtaatgtta aaatcatatc ttggacggtc
     3121 aattttttac tcatctgttt tgcccctttc aatttttaag cgttgctgtc aataagaata
     3181 caggaaagaa aacgacaaaa agtccctatg tctgattaca gacataggga cgaataattt
     3241 cgcggttcca ccctacttcc gaataaagat tcggcagctt catttatcgt actcgcaagt
     3301 gcctcttcaa aaatcgtttg ggtccggctt ccactctccc gaactcgcta aaccacgtgt
     3361 tttttactga tcttggtcaa tgtacgtatt cattttgcag ttttctggtt tgtataatag
     3421 atcctctcag tgaacgatct atttaatcta cggttaataa tagacggaag tccctgtatt
     3481 tgtcaaatag ttttgatttt tttgttgtat ctttcattta ttcttctttt tcaggtatct
     3541 ctttttcagg catatttttc tctggcgtct ttaatacgtg tgcccaagac ttcatttgat
     3601 caatgaactt tttgcttttt agatgaatac cgacatattc atcatacaac tggtcaatga
     3661 ggtcacggat tgcctgcttc gtttcttctt tcaagtggat tccactgatc ttatcataag
     3721 aaatagccga aaacattcga acgaaataaa cagcgcgagg atctgcatga taacggtgtt
     3781 cgtccattgg ccagtgtttc tcgcatatca caccgccata tttggaagaa tagtcaaatt
     3841 ttccttttgt ttccccgcat acgacacagt gcgtccaaat tggcgcgatt ccgaagcgtt
     3901 gcagtatttg cacttcaaag atattcgtga tgatttctgc atcatctcct tggtccatcc
     3961 gctgcagagc ttgttgcgta aattggaata aatgcgggtc atattgatga tcttcgattg
     4021 ctgcatctac tagatttagc aaatacgtac cataaccgct caaaaagata tcttcttgga
     4081 tgttccgaaa cgcatggacg tctttgacac tgcttaaaaa cgacaaacct tcacttcgaa
     4141 aattaccgat atagactgct tcggtaaagg ggagcaaagc cggagctaat ggctgatttt
     4201 gccgatggat tcctttgata taaaacatca ttttgccaga agattccgta aagattttga
     4261 ctaatttatc tttttctttg taattacggc tagataaaat caagcctttc gattctgcaa
     4321 gctggctcat attctccctc ctaaaaaata aatctggaaa agcagattct gccgatccag
     4381 attatttatg tttcgatctt ccgctgtttt tctcaacgta cggattttta tccagtttat
     4441 ttctatacct aacctataag aagtccagaa tatcagtact cgtcttttcg gtagccgaag
     4501 tcttgcagat agactttttt gtctcgccag tctttttgga ctttgaccca aagttctaga
     4561 aatactttgt ctcctaaaag attttcgata tcctttcttg ctttcgttcc tacttgcttc
     4621 agcatttttc cgcctttgcc gataatgatt cctttttggc tatctctttc gacgatgatc
     4681 gtggcctgga tatggatttt atcattctcg tttcgcttca tcgaatcaac aacgactgcg
     4741 acagaatgag gcacttcgtc tcgtgttaaa aacaagactt tctcacggat caattcagaa
     4801 acgatgaagt attcaggatg atcagtcact tgatcatctg gaaaatattg tgggccctct
     4861 ggcatttcac tgactaatac atccattaag cgttcgacat tgtttccttc tgtagcagag
     4921 atcggaatca cttgtgcaaa aggcatctgc gtcgaataat cttcgatgat tcccagcaac
     4981 tcgtctggat ggatcttatc gattttattg ataatcagat aaacaggaga atgcacgttt
     5041 tttagacgtt caataataaa atcgtctccc cggccacgtt tttgatcagc actgatcata
     5101 aataagactg catccacttc tttcaacgca ctgtaagctg cttctaccat aaaatctcct
     5161 agacgatgct ttggtttatg gattcctggt gtatcgataa aaatcagctg agcttctggt
     5221 gtagtatata ctccttggat tttatttcta gttgtctgtg ccttgtcact cataatggca
     5281 attttttgac ccacgattcg gttcaataat gtggattttc ctacattcgg gcggccgacg
     5341 attgcgacaa accctgattt atgttctggc atatacgttc tcctttttaa ttaaacaaca
     5401 aatgatatat tttagggaca aataaaatca acccaatgat gacagcaaaa caagcggtca
     5461 ctaaaactgc tccagctgcc atatctttga tttttttccc aatcggatga aaatgaaaat
     5521 ccgtaaacat gtccactaca ttttcaaaaa cagtattgat gatctctact atccaaacga
     5581 gaaaaacagc taaaagcagc cataaccact ctatccgatc aagctggaaa atcacgccca
     5641 taaataaagc aaggataccg aacgcaacat gtttcctcat attgcgttct tcctcaaaaa
     5701 cggttttgac tccttggaca gcaaattcca cagacatcag gaaatgtttg tttttctcta
     5761 cgtttttatc ttttgagtcc ataggcatcc aagatctctt tctgtaaacc aaacatttct
     5821 ttctcatctt ctggtgtcat atggtcatac ccgttgatat gcaagaatcc atgtaccgct
     5881 aaaaagccaa gttcccgatc atatgaatgg ccgtaagctt ctgcttgttc agcagcacgt
     5941 tcaatagaga tcatgatgtc tcctagtaca cgaggtaatg gattctctcc atcttcaaaa
     6001 atcactggga tttcgtcttc gccttcttct tccagagcaa aactgatgac gtctgtcggt
     6061 gcatcttttc cacggtaatc acgattgatt acttggattg ccgcattgtc catgaaagta
     6121 acagacattt ctgtttcctc tggcagcttt aaataagatg ctgcaaattg cagcaagtca
     6181 tcgatttctt tcattttctc ttctgagact tggtttgttt catccataaa ggtaatatcc
     6241 attatacatc gcttctttct attcttcttg ttcttccttt atcttctcaa cttctgtttt
     6301 cattttagga tatttgatcc gagaatggaa ggtgctactt aaaccgctga ttaatgattt
     6361 ttccactttt cggatttctt tcaaggtcag tccactatcg tctaattgac catcggaaat
     6421 acgctcttcg ataagattat ggacaaaact ttcgattttt tcgctcgtcg gatgatccat
     6481 tgcccgtact gcagcctcac aactgtcagc aatattgacg atccctgctt cacgactttg
     6541 cggttttggt ccaggataac ggaattgctc ttctgttact tccggattgc gttctttcgc
     6601 tttgatgtag aaataacgca ttagcgtggt tccatgatgc tgtttgcaaa tatcgatcac
     6661 catttgcggc atattataat cttccaatat tttggctcca tctgtcacat gtccaaagat
     6721 aatatgttta ctgtcttctg gcaataagaa attgtgcggg ttttccgctc ctgaaggcaa
     6781 gttttcaacg aaaaagctag catgtttgat cttgccgata tcatgataat agcaagctac
     6841 tcgagttagc aaagaacgtc cgccgatttc tgctacagcg ttagcactta gattagctac
     6901 catcatacta tgatgataag tacctggcgc ttcttccagc aactgtttca ataaaggatg
     6961 atttggattg ctgagttcat ttaatacaat tacactgtca tcattgacca tcaattcaat
     7021 atatgggtgt agaccgatcg tcagcaagaa tgagaagagt gcaccagtga acccgcagac
     7081 gagcattaac caagtcgttg catcaccaaa gctcattcct tgatagacga tcaaaatgac
     7141 atccatcatc acaggaaaaa taatgaccca catggcagca gataatcctt gttctgagat
     7201 ccgtttgcgt ctgacaatcg ttgccatgag cccagagaaa acataagaca tcaagataat
     7261 cgttagaaag ttcgtgccaa tcgaatcata aaaaatgaaa agtgcggata ctgcttggaa
     7321 aataccagcc atgatccctg ctcttcgatt caagaaaaag cttaaaacaa gtggtgcaaa
     7381 agcagcggga taaaacagcg ggatataagt agcttgttcc gtttggaata attggaaaaa
     7441 tttcatcagc actatgctga ctgacatagc tgtcacatag aaaagcacgt attttgttct
     7501 ttctcctgca ttttggaatt gaagtgaatt ataaatcagt acgccgattt gaagcaaaac
     7561 agctaaaacc atggcaatta gcggaaaaat cgaagttgtt tgattagtca gtcctagtaa
     7621 ttccaatttt ttcatagctg aagcatcgat ttgattacct tcacgaacaa tgatttctcc
     7681 ttggaagatc atcactggtt gtacagcttc tcgtgcggat tgtttcaatt cttctgtctt
     7741 tttctcattc aatacgtcat tcactacgat accttgatcc actaagtagc gaatcatttg
     7801 ttgttgtgct ggtgtgacat tcaaatattg gatttgctct tctgcttctt gtttaaaagc
     7861 ttccaattca ctttcacgaa cttgtttgct catctgttcg tcaatgagct tcagactctc
     7921 atccctcaca gtatgaatct cagtttcagt catttcaaat atcgtcgaat aaaaattgtt
     7981 cggtagtttt tgataaaaca caacattttc tgcattcaca ttttcaaaat ttttcttcag
     8041 cgcggcgatc cgctcttctg tagtaggctt tgggatattt tcatcttcct ttgccttatc
     8101 aatcttttcg tcataagatt tgttgatggc atcattcgtc ttatcgatca attcaaatag
     8161 ttgcttgatc cgattgttct ggtcatctgc aagatctttt tggtaagtgt attctggagt
     8221 gactgcttct gctgccagtt ttcttttttg ttcggtttct tccgtgttct cgatcgtttt
     8281 gttcgcacgg atactttctt ctgctacttg gccttctctg aaatcattgg ccttttgttt
     8341 gacactacta aatgtaacgc cgcagataat gataaaaaac aagaacagca ggaaaggcaa
     8401 tgctgccttt ccaagttttt cttgtagttt ctgcagtgtt ttattcaaca tgtgcttatc
     8461 ctcctaagca aagcttattc ttgatggaga tctgcctttt cataagcttt gatgatttcg
     8521 gcaactacag ggtgccgaac aacgtcactt gcttcaaaat taacaaaatc gattttcttg
     8581 atttgcttta atgttcgttc tgcatgaaca agaccacttg tcgtccctcg aggcaagtct
     8641 atctgacttg tatccccatt gacaatcatt tttgattgat aaccgagacg ggtcaagaac
     8701 attttcatct gagcgactgt cgtgttttgt gcttcgtcca agataacgaa tgcatcatcc
     8761 aatgttcgcc cccgcatgta agctaaaggt gcgatttcga ttacgcctcg ttccatcaat
     8821 cggttcgtat gatccaatcc aaaaatttgg tataaagcat cataaacagg ccgcagataa
     8881 ggatccactt tttcttttag atccccggga agaaagccta gattttcacc tgcttccaca
     8941 gcaggccgcg tcaaaatgat tttttgcact tcaccttttt tcaaggcagc gattgctaat
     9001 actactgcaa gaaaagtttt tcctgtaccg gctggtccta cgccaaatac gatatcatgt
     9061 ttggctaccg attcgacata tttcttttgg ccgctgtttt ttacccgtat aggttttcca
     9121 ttacggtctt tgacgatctc ttcctcatac atctcaacaa aataatctaa tgtacctttt
     9181 tgccccattt tcaatgccgt cacaacatcg ggcgtactta cgtggattcc tcgtttgatc
     9241 aattcctgta atgtccgaat gactgaggca gccaaggaaa tctgggattg ctcaccaatg
     9301 atctgaatga tttctccacg tgtatggatg gttgtttccg ttgtttcctc aatcagtttt
     9361 atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat cagcagccgt caatctaatc
     9421 tctaaagaac tagactcttc cgtcaaaaat agacatcccc tttttttcga ttcattcttt
     9481 cctattttac catactcaga agaaactata tagcaaataa gaaaaggttc taaaagagtt
     9541 gcttaaccat aagtaccaag aaacgttaaa aaactagccg cttccattct ctaaaggtct
     9601 cgtttgaata aaaaaagctg atcatgtaag ctatgcataa gatagaggac aagtattccg
     9661 tctaacttag caagtattct ctgggtattt ttcttgtttc aaaaaaagaa aacaaaatat
     9721 ttgaaatatt cttgcatttc aaaaaaaaca ggtgtattat aacgtcaaca aataaatata
     9781 cagcaaagcg tcgaggaaaa cagtaggcta ttcagtgatt tattcccagt gagtcatgta
     9841 agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat gtcgaaaacg
     9901 tagatcattg tggctgcctc cattatcagg ctagcggata actttatcga aaagcgtccg
     9961 tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc tagagccctt
    10021 ttcccaagtg gaaaagggct ttttttatat tttaaggtgg tggttttcat ggaatctgat
    10081 attagtggta tttctagttt ctgcaacgtt tattttgtta ctcaaaatga taattattgg
    10141 aggaaaaatt tatggaagaa acaaaaactt attcatggaa gcaattattg atggtcagtt
    10201 cacttatctt cgggatgttc ttcgggtcag gtaatctgat ctttcctgta cacttaggtc
    10261 aaatggctgg atcaaactgg atcagcgctg gtgtaggatt tgccatctca ggcgctcttt
    10321 tcccattatt agctatctta gctgtagtag tgacaaaaag tgatggtctg tatgacttag
    10381 ctaaacctgt cggaaaatgg tatgctgcct tgttcttgat ccttgttcat ttaacgatcg
    10441 gaccattctt tggtacaccg cggacagcag caactgcttt tgaaatggct gctaaaccct
    10501 ttttgcctga aaaatatgat tcattaggta tgtttttatt ctctgctctt ttctttttac
    10561 ttgcttattt ccttactgta catccaacac gtttggtcaa atatgtcgga aagtatttaa
    10621 actccttgtt tcttgcatta ttagctatca tttttgtcgt ttctttattc caacccatgg
    10681 ggaatctaaa ccaatctgct gcagattctt atcagacgaa cgctttgaca aatggcgttt
    10741 tagaaggata caacacggtt gatgcagtag ctttacttgc tttaagtgtc acttttgtcc
    10801 atgcagttcg tgatcttggt tttaaagata aaaaagtcac agaattaaca gcaaaagctg
    10861 gtagcctgag cgttgcgtta gaagtcatga tttattttgg tctggtcctt ctcggtgcga
    10921 tgagtttgaa taagatgtct ttatcagaaa atggcggaac tgcactttct cagatcacta
    10981 atttttattt aggtaacttc ggtgctagct tcttaggggt aatggtcaca ttaggtgtct
    11041 tcactactgc tatgggactc gttgtttctt ttgcacaaga tttccataaa ctgttcccta
    11101 aagtcagtta tatgacatgg ctgcggttaa cgacttttgt ctcttttgtc gttgcgaatg
    11161 ccggattgga taatatcatc caatggtctt taccagtatt gatgcttttg tatccattgt
    11221 cacttgcgtt aatcttactt tccttaacag caaaattctt ccaaaagagt ccatttgtct
    11281 atcaagtaac gatgttcttt tctgccgttc cagccgtatt ggatatgttg gcaaattcgc
    11341 ctgcattagt gagtcagcaa actgtcgtag caagcttact aggattttat catcattatg
    11401 ttccttttgc tgcattaggt cttggctggg tcgtcccaac gttgcttggt tacgctggca
    11461 gcttgctctt ttattatgta tatcgcgtat caggatataa acaagaagca agtgagttac
    11521 cagaagaata accaaatcaa acacaattta acataagaga tattaaacag gaaaaaaaca
    11581 gctagaagaa agaataagat tctttcttct agctgttttt tagttctttt tttacgaaca
    11641 caaaaaactg cggataatca ctgtccgcag tttaaatgat aaaattaaga ttgtaatagt
    11701 tctttcacaa tcgcattcac ttggtttcca tctgcttttc ctttgacttt aggcataatt
    11761 gcacccataa ctttgccaaa ttctttcatt gatgaagcac cagtttgagt gattgcttct
    11821 tgaacaagtt gtcgaatttc ttcgtcggaa agttgttccg gcatatattt ttcgactatt
    11881 acaatttcac tttttacttt ttcagctaaa tcgtcacgtc ctgcttcttc aaactcatgc
    11941 aaagaatcac gtcgctgttt catttcacga gacaacaccg ttagttcttc ttcttctgtc
    12001 aagtcgtgac cttctttaat ctgctcgttt tgtatagatg acttgatcat tcggatgact
    12061 tgtaaagatt ccttatcttt cgctttcatc gctgctttca tgtcatcgtt taatctactt
    12121 agtagtgaca cactaccaac tccaattcat cacagaacta gaattttttg cgttttctag
    12181 ctgcttcaga ttttttcttg cgtcttacac ttggtttttc gtagaattca cgtttacgag
    12241 attcttgtaa agtacccgct tttgacacgg aacgtttgaa gcgacgaaga gcatcgtcaa
    12301 gtgattcgtt tttgcgaacg actgtttttg acatgttaat tccctccctc cgagcttaaa
    12361 aagtaaccgt aattgttatt gaaatatatc tcaaaaaaca agactgtcct gtaaatatta
    12421 tatctaatgc tgttttgacc gtcaaggaaa tcttgctttt ttttaaaatt atcaaatgtt
    12481 attttattta aatccttatt gacaattttc acatcttccg aaaatctcga atctatgccc
    12541 ttcgatcgtg catccaccta gctgttcttc gaaataattc atcggacaca tatggatttc
    12601 tttcgttgtt ccgcacactg tacagataaa atgatggtga tggccgattt tcccctgaca
    12661 gcaatgaaaa cgaaacttca tttctccatt taaatcggtt tcttccagta aatcgatctc
    12721 tgcaaaatca tggagattcc ggtaaatcgt atcataactt acccctttga attcctgatt
    12781 catatagtca aaaacttctt tagcagatac gtagcggtta cgcttcacta aataagttaa
    12841 caatgcttct ctttttttag tatatttaaa accgttattt ttcaatgttt ctaatgattg
    12901 ttcgatcaat gtctgttttt tcatgactta ttcctccaaa tcaaaatgat tacgaagtac
    12961 gcattctaga gtatggtata ataaacataa aaaaaatgca agtgaggttt tatccatgtc
    13021 ttccgattct acagaaatcg ttactttttt tgtcatctcc gactctgctg gagaaactgc
    13081 gacaaaacta gcgcaagcta ctatggctca gtatcctacc gttgagttca acctattccg
    13141 cagaacgttc gtgacagatg aaaaaacatt aaggcaggca ttgcaagatg cattagaaga
    13201 aaaagcactt gtcctgcata cattaatcaa tcaagaactg atcgagataa cacgttcttt
    13261 ttgtgaagaa catcaattat ttagttttga tgtgatgacg ccaccgatat cagaaattga
    13321 acgtttaact ggtatcaagc caattcggca acctggtgcc ctccatttat tgaatgagaa
    13381 ctatttcaaa cggatcaaag caatggaatt tgctgtaaaa tacgatgatg ggaaagatcc
    13441 tcgtggtttt ttagaagcag atgtcgtttt gctaggcgta tcccgaactt ccaaaacgcc
    13501 tttgagcttg tttttagcta acaaaaattt aaaagttgcc aatttacctt tgatcccaca
    13561 agcacatatt cccaaacagc tttgggagat cgatcctaaa aaaatcgtgg gactaacgaa
    13621 taacccagat attttaaata atatccgtaa agaacggatg cgctcttacg gattaaaccc
    13681 tgatactgct tattccgata tcgaaaaaat tagagctgaa cttgattttg cgaatgatct
    13741 ttatgaaaaa ctaggatgtg tagtgatcaa tgtggcatct ttatctattg aagaaacggc
    13801 ttcgcttatt ttgaatgctt tagacctaga agatcatagt tattatggaa ctgaaacaac
    13861 agaaaaaagc gattaatcat ttttgtcttt ttcttaaaaa acactggagt gtgacaacga
    13921 agaaatttcg ttgtcacact ccagtgttta tctatttgca cctatcgtta ttgatgttcc
    13981 acatgtttga gtgtctggtt caaaatatcc agtacatgag aatcatcaag actatacaaa
    14041 atcgcttttc cctcgcgatg agacttgacg acatgatttt ctcttaataa acgtagttga
    14101 tgcgatacag cagactgctc catttccact gcttgggtga ttgctgtcac attttgttcc
    14161 ccgtctttca gatatagcag aattttaaga cgagtggggt cactcaacat tttgaataat
    14221 tgactgactt tctctacttt tgatttttgc gtgtctggca atccattcac ctccaacttg
    14281 aaaaaagact gacttctaaa gtttaccact ttttccatca gtctcaagtt ttttattctt
    14341 ctgtcatctt tttgataatc gcttcataag aggctacttt ttcggctgcg tcttctgtag
    14401 agcttccttt tacaccaata taaaacttga ttttcggctc agttcctgaa gggcggatag
    14461 cgatccagcc attttcttcc aatgtatact tcaatacgtc ggatggcggt gttgttagtt
    14521 tttctacgtt tccttcacgg tctgttctcg tcaattcttt aaagtcttct gtttgaataa
    14581 cttttgctcc ggcaaattct gtcggtgctt ctttacggaa tttttccatc aaagcagaga
    14641 ttttcgcaga accttcttgt ccgctcattg tcacagaaat cgttttttct gcgaaatagc
    14701 cataggtttc aaaaatcgtt tgcaagccgt catacaacgt tttgccttgt tttttatagt
    14761 aagctgctac ttctgctaat aaaagcaatg cttggatcgc gtctttatcg cgaacaaaag
    14821 gtttaactaa ataaccataa ctttcttcga atccaaacat aaaagtatgt gaatggtctt
    14881 cttcaaattg ttggattttt tcagcaatga atttaaaccc tgtcaacaca ttgaacatag
    14941 tagcaccgta gtgttccgca attgctgtag gcaattcgct ggaaacgatt gatttcaata
    15001 ccgctgcgtt ttctggtaaa gtgcctcttg attgatgtgc ttccaaaata tattgagcaa
    15061 acaatgctcc gatttggttc ccagtcaata cttcataaga accatctggc aaacgaaccg
    15121 ctgcgcctaa acggtctgca tcaggatctg ttgcaatcag caagtcagct ccctcttttt
    15181 ctccaagtcg tatagcatat tcaaaagctg aatgttcttc tggatttggc gatttgaccg
    15241 tagtgaaatc tggatctgcc acagcttgtt cgggaactag tacgaattgt tcgaatccag
    15301 cttgtttcaa cgcacgttcg cccaacattt tccctgttcc atgaagtggc gtgtacacaa
    15361 gtttcaaatc tttccccatt gtttcaacca attcacgatc aatcgtcact gctttgattt
    15421 cttctagata agcagcgtcc acttcttctc cgatgatcgt gatcaacccg ctatgtttag
    15481 cttcttcgtc agataatact tcgactttta gcggattgct gacttcacgg acatatttcg
    15541 tcaatgcatc tgcgtctgca ggaggcattt gtccgccatc ttctccgtag actttatatc
    15601 cattgtaagc cgctgggttg tgagatgcgg ttaccatgat tcccgcaaac gtcttcaaat
    15661 gacgaactgc aaaagaaagc tctggtgttg gacgcagact ttcgaataca taagacggga
    15721 tattgtgatg tgccagtgtt ttggctgctt ccattgcaaa ttctggtgac tgatggcgtg
    15781 agtcataagc gatagcgaca cctcggcgtt ttgtttcttc accttgtgta tccatgaaac
    15841 gtgctagtcc ttctgttgct tgtcttaccg tatagatgtt cattcgattg atcccaggtc
    15901 ctaagattcc ccgcatgcct gcagtaccga attccaacgg tgcataaaaa gcatcttcaa
    15961 gtttttcagg atcttcaccc agatcattta attgtttttt taagttttct tctaaatttt
    16021 cttcatgtac ccattgttca tacacttgtt cccaagacat gaaaataaca cctctttctg
    16081 aaatatggtt cttaataagt atagcattca cttattagat atgaaagtat gaggaccaaa
    16141 aaacgcttaa atcaagaaag gattctttta tcaagcatga ttgcaagtat tcacataaat
    16201 cggattcagt ggatttcacg cgaaaaaaaa gagtaaaaga acaattgaaa ccgttctttc
    16261 actcttttta aacttatgct gttgtttctt ctaaagactc caagtattta tacacttgtt
    16321 gtccagcaat accgccgtca ccaacagctg tagtgatttg tcgcaaatct ttttcacgaa
    16381 catctccgat ggcaaaaacg cctggcatat tcgttttcat ttcgttgtct gttggaatcc
    16441 agccagcttc gttcgtcaaa cctgcttctc taaaaggtgc agttaatgga tccagtccaa
    16501 catagatgaa tactccgtct gctgctaaat caaattcatt gccggttttg acattacgtg
    16561 ctttcacacc tgtcactacc atttcgtttc caacgatttc gtcaacgact gtatcccaca
    16621 taaaggaaat tttttcattt gcaaatgcac ggtcttggat gatcttttgt gcacgtaatt
    16681 catctctacg gtggacgatc actacttcag aagcaaattg tgtcaaatag atggcttctt
    16741 ctactgctga gtccccaccc ccgatgacta ctagacgttt gttacggaaa aacgcaccat
    16801 cacagactgc acagtaagaa acgcctcgtc cagcaaattc ttcctcgcca ggtacgccta
    16861 atttacggtg gatacagcct gttgcgataa tgactgtttt tccttcgtaa cttttatctt
    16921 cacagatgac ttctttatgg ctgccgtgat ccttgatatc ttgaacgatt ccataagcat
    16981 tttctgtccc aaatcttgag acactttcat acatcttata agctagatcg gggcccatga
    17041 tcgaatcgaa tccaggataa ttttcgattt ctgccgtatt gttcatttgg ccgccagggg
    17101 caccttgttc gatcatcaat acggataagt ttgaacgtga agcatacaaa gcagcagtca
    17161 tcccagcagg acctgcgcca ataacgatta catcatacat atgtagattc tcctttactt
    17221 aatcaagtaa gcatacttta cagccaatgc gaaaaaaagt ctattgttct gcttgcgttt
    17281 ctcgaatagc tttattatac tcgaaaaatc cgctgaaaaa caatagactt ttataaatca
    17341 ttgatcaatt gttcgaaaga ttttctccat ttgaatcaat tactttttta taccagttga
    17401 atgatttttt ctttgaacgg gctaatgtgc catttccttg gttatcacga tcgacataaa
    17461 tgaatccgta ccttttcttc atctcaccgg taccagctga tactaaatcg atacatcccc
    17521 aagtcgtata gcctaataga tcgactccgt ctagttctac tgcatccttc atcgcctgga
    17581 tatgcgctgc caggtaatcg attcggtaat catcttccac ataaccattt tcatctggcg
    17641 tatcgactgc acctaagccg ttttctacaa taaataatgg tttttgataa cgatcataaa
    17701 tatcattcat cgttattctc aagcctaatg gatcaatttg ccacccccat tcacttgctt
    17761 gcagatatgg atttttgaca gaagcaaaga tatttcctgc agtctgttcc aagagttcgt
    17821 tgtcggtagt cgaaactcgt gaagaataat aagaaaaaga gataaaatca acagtatgtt
    17881 ctttcagcaa tgcggcatcc cctggttcca tttcgatctg aatgccattt cgctccattt
    17941 ctttcaatgc ataagcggga tattctcctc gtgactggac atcaatgaag aaataatttt
    18001 ctcgatctga ttttctagaa gcccaaacat cttcaggatt gcaagtgtat ggataatatg
    18061 ctcctgctgc cagcatacag cctactttgt tctccggatc tacttcatga gcaatcttcg
    18121 ttgcgatggc acttgcaacc aactcatgat gtgccgcttg atatttgatt tgttcttcat
    18181 tttctccttc ttcaaaatac agccctgcgc ccataaatgg tgcatgaagg atcatattga
    18241 tctcattgaa tgtcagccaa tatttgacaa gtcctttata gcgattgaag atgactcgac
    18301 acagattttc ataaaaacca acgagttctc tggaacgcca cgcaccatat tctttgatca
    18361 aatgcattgg acaatcaaaa tgcgtaatcg tcacaagtgg ctcaattccg tgcttttggc
    18421 attctttaaa gacatcctca taaaatttca agccagcttc attcggctct ttctcatccc
    18481 ccattggaaa aatacgactc caggcgatag acattcgata agtcttgaac cccatttctg
    18541 caaacaaagc aatatcttct ttataacgat gatacatatc gattgcaatt tttgctggat
    18601 aaaaatgatc ctcatcaaat tcaaacattt ttttctttcc ggtgataaca ggaaaacggt
    18661 cttttccgat tggcacaaca tctacgtttg ctagtccgcg tccaccttca tcataagcgc
    18721 cctcacattg attggctgct gtagcaccgc cccataaaaa atcttttctg aaccccatta
    18781 tacttcctcc attatatgtg tattacctga taattctctt gccagttcat cttgaacttc
    18841 gattccttct tcttttaatc ggctgattgt ttccttgaat tgcggtaaat actgctggtg
    18901 tgcaataaac aactcatcca tgatccgttt tgctgtcttt cctgatcgta taagtggatt
    18961 gatcgtaaac gcctgtaaag ctgtcccata atcaccagtc acagctgctt cgatcgtcag
    19021 caattccata ttcttcatta cttgcaacca gcctctttca gcagcaggaa gagaaccaaa
    19081 cgctacattt ctcgctcctt gtgcgccaac ataggcagtg acttctacaa cacaatctgc
    19141 cgacaggtca ggaacagctc catcattctt tgtagataca acgatctgtg tttctttatt
    19201 tccatagatt gaagcaatcg tttcacaggc agcgtctgaa taataagcgc cacctcgttt
    19261 ttgcaattgt tcaggtttat ggtctaattc tggatctttg tataattcaa atagctctgc
    19321 ttctgtttct ttcacttgct gcgctcttgt accaatcgtt tgatattctt ctaaagcatg
    19381 ttccagcatt tcttcttcac gatagtaata gcggtggtag ccacaaggga tcatgttcat
    19441 ttgttttaat tgttctttga aaaacgggat atcaaagata tttttcggta atccattatc
    19501 ttcatcgtat aaacgatcaa tcaattctgc cgtgcggtca tttcctttat tatctgctac
    19561 tttgtgccaa tggaaatgat ttagaccggc aaacttgtag atcagctctt cttttttctt
    19621 gccaagcatt gctggttctg tcatcatcgc catcactggc acattgcata atccaatcac
    19681 tttatcccat ttgccatatc tgataactgc ttctgtcacc atcccacttg ggttcgcgaa
    19741 attgatcagc caagcatttg gacataaacg cttcatgtcc tcaacgattg ccaaaacgac
    19801 tggaacggta cgaaaagctt tgaatatccc gccagcacca tttgtttctt gcccaatcat
    19861 cccataataa ctagggatcc gctcgtcttt gattcgtgca tctaattgac cgacacgaaa
    19921 ctgagtcgta acaaaatccg catctttcaa tgcttcttcc cgatccagag ttgcatagat
    19981 cttgacttca tatggagaag catcccacat acgctgagac atctgactaa cgatctcaag
    20041 cttttctttt ccttcttcta catctacgag ccagatttct cgtatcggta attcctcata
    20101 tcttttgata aatccttcca tcaactcagg ggtgtaactg ctgcctccac caatcgtagc
    20161 gattttgatt ccttttgcca tgttctcact cctttacttt tttacacttt cattatcttt
    20221 tttgtaaaca aaacaaacaa tagaaatccg tttctttcca gtaacattta caaaagttac
    20281 tgttttctag tggatttttg ta
//
LOCUS       DAXWLB010000041        20157 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXWLB010000041 DAXWLB010000000
VERSION     DAXWLB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.041"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(38..565)
                     /locus_tag="ACGE4H_001122"
     CDS             complement(38..565)
                     /locus_tag="ACGE4H_001122"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8364554.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(632..2134)
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_001123"
     CDS             complement(632..2134)
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8364555.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2392..3513)
                     /locus_tag="ACGE4H_001124"
     CDS             complement(2392..3513)
                     /locus_tag="ACGE4H_001124"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8364556.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3639..4580
                     /locus_tag="ACGE4H_001125"
     CDS             3639..4580
                     /locus_tag="ACGE4H_001125"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8364557.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4730..5626)
                     /locus_tag="ACGE4H_001126"
     CDS             complement(4730..5626)
                     /locus_tag="ACGE4H_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFF8364558.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5895..7235
                     /locus_tag="ACGE4H_001127"
     CDS             5895..7235
                     /locus_tag="ACGE4H_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8364559.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7453..7896
                     /locus_tag="ACGE4H_001128"
     CDS             7453..7896
                     /locus_tag="ACGE4H_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFF8364560.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8400..9818)
                     /gene="nhaC"
                     /locus_tag="ACGE4H_001129"
     CDS             complement(8400..9818)
                     /gene="nhaC"
                     /locus_tag="ACGE4H_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFF8364561.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10221..11444)
                     /locus_tag="ACGE4H_001130"
     CDS             complement(10221..11444)
                     /locus_tag="ACGE4H_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8364562.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11475..12314)
                     /locus_tag="ACGE4H_001131"
     CDS             complement(11475..12314)
                     /locus_tag="ACGE4H_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8364563.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12331..13011)
                     /locus_tag="ACGE4H_001132"
     CDS             complement(12331..13011)
                     /locus_tag="ACGE4H_001132"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8364564.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13013..14086)
                     /locus_tag="ACGE4H_001133"
     CDS             complement(13013..14086)
                     /locus_tag="ACGE4H_001133"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8364565.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14142..14380)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14423..14656)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14733..15404)
                     /locus_tag="ACGE4H_001134"
     CDS             complement(14733..15404)
                     /locus_tag="ACGE4H_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8364566.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15491..18139)
                     /gene="mgtA"
                     /locus_tag="ACGE4H_001135"
     CDS             complement(15491..18139)
                     /gene="mgtA"
                     /locus_tag="ACGE4H_001135"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFF8364567.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18481..18655)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18828..19265
                     /gene="ndk"
                     /locus_tag="ACGE4H_001136"
     CDS             18828..19265
                     /gene="ndk"
                     /locus_tag="ACGE4H_001136"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFF8364568.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19287..20153)
                     /locus_tag="ACGE4H_001137"
     CDS             complement(19287..20153)
                     /locus_tag="ACGE4H_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8364569.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         5763 a         4217 c         3566 g         6611 t
ORIGIN      
        1 ccttgaaata ttttatttaa ataacacaaa aacataatta ctcataagaa accaattctg
       61 tattatctac catgaatgta aagaacccct cattaggcat cggatgaaaa taagcattga
      121 tcactcttat caaggcatta tggcaaacta acagatagac attttcttcg tcttcgatgc
      181 attctttcaa taaaggaacg atccgtgcat acacatctaa gactgactcc ccatttggga
      241 atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc atctttgctt ggcagtccat
      301 cataatcccc aaaatccatt tctaccaatc gttcatcaac ggtcagcggc aagctgagac
      361 gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag aggggaatgg atgattttcg
      421 tgattggaac aggcaaatct actacctttt ctgctagttt ctgtgcctgc atctgtcctt
      481 tttcagtcag cggcacatct gcccgtccgc aaactaatcc ttgtgcgttc caagttgttt
      541 ctccgtgtct cgttacataa agcatgtgat tctctccttc gttggcaaag ttccccttaa
      601 ccataccttg ttgttcttga gattacaagg gttagattct ctcaagagaa ataatctcag
      661 tagatacttt atccaggaag gtttgatcat gttccactag taacatggca ggtttcacgt
      721 tcaagatcag ttgttctaat tgttcttgat tgaagacatc caaataattc aatggttcat
      781 cccatgtata tagttcagct ggctgtgata aagatttagc caattccact tttttccgtt
      841 ggcccatact catctgctcg atcttgttat gaaaaacatc tctttccatc ccaagctttc
      901 ggaggttgtt caaaaatgct tggtagtcta cttggttttt ctctgcaaat tccgccaacg
      961 ttcctcgatt gtcttcataa ttttggcttg cataactaat gctcagatgt tttggctggt
     1021 atttttctcc tataaccttg ccgttgaatg cccccagaag ataatggata atggatgact
     1081 ttcctgcacc gttaggacct gaaatcgcca cccgctgatg aggctcgatt gtaaaatgaa
     1141 ttggctcgaa taacagattt tcatacccta attgaagatc ttctacgctt aaaagtcgct
     1201 tatggtgaga cgcttggcta ttcatcgtca aagaatcgat atattcgata tcttttaata
     1261 gtttttcttt ctcgctgatc tgggtctcca tccgattcac gattgctttg gatcgtttca
     1321 tcgtccgtgc agcatcagca cccactgctc ctttattcac tcgtctagat tcagtatcga
     1381 tgaatccgac ttgtttcttt gttttatctc cttctcggga acgagaccat tcggcttttt
     1441 cagctgctgt tttctttagc ctactgactt cttttttcaa tttttcattt tgagccattt
     1501 caaattcatc acgaagtttt ttctgttctt catagataga gaaattccct tgataaagtt
     1561 ccagttgact tttttcgatt gctaaaacat ggtccactac ttcatcgata aatccccggt
     1621 catggctgac cacgatgaag ccttgttttt tctttttcaa ataagccgct acttgttttc
     1681 taccagagat atccaaatga ttcgttggtt catcgattaa cgggaaatga gtgtcatcca
     1741 caaataaaag tgccagtagg acttttgtct tctctccccc cgatagtgtg ctgaattctc
     1801 tccataagat ttcaggatct gtttgcatca attggagctc tctttcgatt tcccatatct
     1861 caaaatccgt aatatcattt aacacgtaat aggttaaacg ttctttatct tttgtctgtt
     1921 gcgggaaata ggcaaattct tgctgatgga ttacttgccc ttgataaggt agtttgtttt
     1981 gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg tccgataagt cctagtttcc
     2041 attgtgtgtc aaaatttaga tttgcttgtt caaataataa tgtgccttgg ctgtcgtagc
     2101 cgaatgtcag attttttatt tcgattttag acattttcca tcacctcgta aaaaaagacc
     2161 agtcctattt tcagaactgg ctaaagagga tgcaagggtt ggatacacac aaaaagaccc
     2221 atacatcgaa aaaaccaagc caatcccgaa aatctgcaca ctgcttcctt gtctccacaa
     2281 ggtacagtct gaatgcagct ttcatcaatc gacttaattt ttcaatgcac ggatccctca
     2341 ctttgtccta tatttgaaat tacgatagca taaagaaaat gattttgtca actacttcgg
     2401 gaggatggtg aaaaatctgc taagtgtctg tcctaagatt tttgtttttt ctaatgtttg
     2461 cctgtgttcc attgcttctt ctaaacctat tggacccaaa tgaatactta agacaattgg
     2521 gagaaaatca gaaagtcctt ctagctcttg gtctctactt ccgcaaatag cgattattgg
     2581 ttttcgatat ttttttgcaa gcactgctac accgtatgga acttttccgt ggatcgtttg
     2641 ctgatcgatc cgcccttctc ctgtaatgat gacatctgct tgttcgatct ttttttccaa
     2701 tccagtgatt tggctgacta aatcgaaacc agaaaccatc ttcgctccca acaaaaaaag
     2761 ggctccacca ataccgcctg ctgctccact tcctggaata tcatctaaat ccacctccca
     2821 gaaatctttt atttgctgag caatctttcc tgcttgttta tctaagaaag ctagcgtcgt
     2881 atctattcct cctttttgtc ttccaaattg ccaagcagct ccttgttgac ctgtatatgg
     2941 attggtaaca tctgcagcga taatcaattt tactttagaa acaagaacgt ttcttcgatc
     3001 aaaatgaagt tttttagtag ataataaagg atttccttct gtgtaccctt ctaaagaagc
     3061 acccaaggat tggagtaatc ctagaccacc atcgctacac ccgcttcctc ctaaagtgac
     3121 aataatttgt tctacttctt gaagcagtgc atctttaata agttctccta aggcataact
     3181 ggaagcaagt cggatagttt gatccgttgg tttatcaagt agatctaacc ccaatgcttg
     3241 tgcagattcg atgactgcgg tgttttttcc ttgccaagtt gtcaataaat agctgatttt
     3301 tcgctgacgg aataagagat ccatcccatc aatggtacgc catgttcccc tgagcgcgta
     3361 atagattgct tctgcagtat tttctccgcc atctgcaatc ggcacattga tttttcttcc
     3421 tttacgatct tcccagccat ttagaactgc ctgatttagt tcaaatgaag aagcacttcc
     3481 tttaaatgaa tcaattgccg ccaaaatatc catctgtatc cctcactttc ttcttaagta
     3541 agacactttt tttcattttt ttcaaacaaa acaatgctat tttcttccat ttttctatat
     3601 actaaaaggg aactcattga ataaaggaga gaaaaaacat gaaaatcgca atagctgggg
     3661 ccggagcaat gggcagccga atcggattga tgctacatca aagcggaaat gaggtcctct
     3721 tgatcgatcg ttggcctgct cacattgaag ccattcgtac aaatgggttg attgctgact
     3781 ttaacggtaa agaagtggtc gcaaaactgc caatctattc accagaagaa atcatcgaaa
     3841 gcaacgaaca cgtggacttg atcgtagcat taacaaaagc caatcaatta gatgatatgt
     3901 tttgttcgat ccaatccatt attactgaca atacttatgt tttgtgttta ctaaatggat
     3961 taggtcatga agatgtactg gaaaaatatg tgccaaagaa aaatatctta tttgggatca
     4021 cgatgtggac agctggtcta gctggtcccg gaaaagtcac attgttaggt gatggagaaa
     4081 tcgaactaga gaacttggaa ccagaaggcg aagcatttac aaaaaaagtt gtagaagtct
     4141 ttcaagaagc aaatttaaat ccgatctata gccataacgt tcgttattcc atttggcgaa
     4201 aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat tttggattgt aatattgctg
     4261 aactaggcgc gcaaaaagcg gcagagagca tggttcgaac aatcgtatct gaatttgctt
     4321 ctatcgcagc aaaagaagga atcatactag atcaagaaga agtgtaccaa catattgctt
     4381 ctacttatga tcctgacaac atcgggttgc attacccatc tatgtaccaa gacctgatca
     4441 aaaaccatcg tttgactgaa atcgactaca tcaatggtgc catttggcga aaaggacaaa
     4501 aatatgatat tgctacacca tattgtgcct ttttgaccca attagtccat gcaaaagaag
     4561 gaatccttgg cgcagaataa aaggctctct gatgagaatc agctgaatca gcagctggtt
     4621 ctcttttttt aagcccaata aaagatcaaa aagaaaacac aactctcatg atgccataag
     4681 aattgtgctt gtttgtttca gtacataatc tcttcaaaat gtaggttttt tatttgtttt
     4741 caacagagga gaggtcatat tcgtattgct cataaatagt ggaatcgtta gtgataaatc
     4801 gttttgccca attttctgca tcataaaatt ccaatagctc tgataggatt ggcttaaatg
     4861 gtgtatgcag aaattccggt aattcatcta aatcacgttc tcgaagcaaa aaaatatttt
     4921 caggacgata aaaatgataa tgagcaatcg cttgattatc cgtcactagt ttcttttgaa
     4981 aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg accctcccgc aacagttcca
     5041 gaatcccgcg cgattcccct gtttttttca ggtactcttc ataagtaaga tatccaggtt
     5101 gttcattccc tcgatgtgga agaatgaaat agttggtcgt aacatctctt tttataaatt
     5161 cctgcttata gctttctgct cgttcttttc tcccattatt cgttgcacca aaaaatagat
     5221 ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga ataaaatgaa ctattatgtt
     5281 tcagcccaag ttcttttgct tccaaaaaat caaaatgata aatctcatcg attgccggtt
     5341 catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt gaagtgattc caaaaaaata
     5401 acacaatttt tgcattggta aatgggcgca cgcgtcgagc catctccata tcaaatccat
     5461 aatctaaaaa aacgatcagc tgataatttg ccaactgttg tctagctgat ttaatgaatt
     5521 ccgactgtag taaatctttt ccggaccaaa catctaaaga gcggatggaa ggcaaaaaat
     5581 aaagtgattt attttttgca actagcagta cgtcattttt gttcatcttt tccgcctcac
     5641 tttctctgtc actttttcct atgatagcct gtttaaatag taaaaagcca gtaaaacgtt
     5701 tcctatattt gtatgaaaat ttaacatttg tatgaaaata tgatgaaata aatcattctt
     5761 ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat tgtctgaaaa tttgttatca
     5821 ttagtcattg ttaaatattg ctatttattc ttaatataaa ttttgattgt gaatttaaaa
     5881 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
     5941 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
     6001 ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg attttatata
     6061 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
     6121 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
     6181 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
     6241 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
     6301 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
     6361 aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt
     6421 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
     6481 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
     6541 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa
     6601 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
     6661 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
     6721 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt
     6781 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
     6841 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
     6901 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
     6961 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
     7021 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
     7081 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
     7141 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
     7201 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
     7261 tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc
     7321 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
     7381 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
     7441 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
     7501 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
     7561 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
     7621 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
     7681 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
     7741 atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta
     7801 aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc
     7861 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
     7921 gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg
     7981 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     8041 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
     8101 cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa
     8161 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
     8221 ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg
     8281 aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat
     8341 accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt
     8401 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag
     8461 attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat
     8521 acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca
     8581 attccccaag gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat
     8641 acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata
     8701 acagatagga attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta
     8761 aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc
     8821 aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta
     8881 ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta
     8941 tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt
     9001 tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc
     9061 acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata
     9121 gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta
     9181 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac
     9241 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt
     9301 gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca
     9361 acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     9421 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat
     9481 acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga
     9541 atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     9601 gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat
     9661 ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt
     9721 gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt
     9781 gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt
     9841 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt
     9901 cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc
     9961 accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt
    10021 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
    10081 aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc
    10141 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
    10201 acacacaagt ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt
    10261 tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct
    10321 gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca
    10381 gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc
    10441 tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt
    10501 tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag
    10561 tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca
    10621 aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact
    10681 ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga
    10741 atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc
    10801 aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt
    10861 actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact
    10921 ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac
    10981 gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc
    11041 tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg
    11101 aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat
    11161 gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt
    11221 ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc
    11281 caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta
    11341 cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat
    11401 tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat
    11461 gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt
    11521 gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga
    11581 tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca
    11641 tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca
    11701 gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa
    11761 gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc
    11821 gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct
    11881 ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct
    11941 acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat
    12001 actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa
    12061 ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc
    12121 acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct
    12181 gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct
    12241 tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga
    12301 ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt
    12361 actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac
    12421 atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc
    12481 tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg
    12541 aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt
    12601 tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat
    12661 ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc
    12721 aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa
    12781 agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc
    12841 tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc
    12901 gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc
    12961 atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc
    13021 ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta
    13081 caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag
    13141 taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat
    13201 tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc
    13261 caatataact taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg
    13321 caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca
    13381 attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat
    13441 cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca
    13501 ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg
    13561 tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg
    13621 ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag
    13681 cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt
    13741 cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc
    13801 tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt
    13861 ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt
    13921 tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga
    13981 ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt
    14041 tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt
    14101 atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc
    14161 gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata
    14221 ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc
    14281 tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc
    14341 aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt
    14401 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
    14461 agtttccttc cctcaagccc aacatctcaa aaagagagat tggcgttcgt tttaacggtc
    14521 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
    14581 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
    14641 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt
    14701 caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt
    14761 tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg
    14821 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
    14881 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
    14941 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
    15001 tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt
    15061 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
    15121 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
    15181 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
    15241 actaaaatat caaaactttg tttccagttt tttccgcccc aagcgtcaac gatttctttt
    15301 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
    15361 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
    15421 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca
    15481 gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
    15541 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
    15601 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
    15661 ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg
    15721 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
    15781 catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc
    15841 ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    15901 aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg
    15961 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    16021 agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac
    16081 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    16141 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    16201 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    16261 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    16321 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    16381 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    16441 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    16501 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    16561 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    16621 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    16681 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    16741 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    16801 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    16861 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    16921 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    16981 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    17041 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    17101 ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt
    17161 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    17221 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    17281 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    17341 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    17401 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    17461 tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac
    17521 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    17581 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    17641 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    17701 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    17761 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    17821 taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    17881 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    17941 tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt
    18001 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat
    18061 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    18121 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    18181 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    18241 agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    18301 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    18361 agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    18421 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    18481 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    18541 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    18601 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    18661 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    18721 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    18781 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    18841 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt
    18901 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    18961 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    19021 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    19081 gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact
    19141 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    19201 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    19261 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    19321 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    19381 tggtaatctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    19441 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    19501 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    19561 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    19621 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    19681 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    19741 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    19801 accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    19861 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    19921 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    19981 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    20041 gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac
    20101 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataata
//
LOCUS       DAXWLB010000042        19702 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXWLB010000042 DAXWLB010000000
VERSION     DAXWLB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.042"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            93..373
                     /locus_tag="ACGE4H_001138"
                     /pseudo
     CDS             93..373
                     /locus_tag="ACGE4H_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(451..984)
                     /locus_tag="ACGE4H_001139"
     CDS             complement(451..984)
                     /locus_tag="ACGE4H_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFF8364570.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1078..2256)
                     /gene="mutY"
                     /locus_tag="ACGE4H_001140"
     CDS             complement(1078..2256)
                     /gene="mutY"
                     /locus_tag="ACGE4H_001140"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFF8364571.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2249..3046)
                     /gene="recX"
                     /locus_tag="ACGE4H_001141"
     CDS             complement(2249..3046)
                     /gene="recX"
                     /locus_tag="ACGE4H_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFF8364572.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3153..4535
                     /gene="rlmD"
                     /locus_tag="ACGE4H_001142"
     CDS             3153..4535
                     /gene="rlmD"
                     /locus_tag="ACGE4H_001142"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFF8364573.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4709..5287)
                     /locus_tag="ACGE4H_001143"
     CDS             complement(4709..5287)
                     /locus_tag="ACGE4H_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFF8364574.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5609..6079
                     /locus_tag="ACGE4H_001144"
     CDS             5609..6079
                     /locus_tag="ACGE4H_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFF8364575.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6187..7413)
                     /locus_tag="ACGE4H_001145"
     CDS             complement(6187..7413)
                     /locus_tag="ACGE4H_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8364576.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8167..8367)
                     /locus_tag="ACGE4H_001146"
     CDS             complement(8167..8367)
                     /locus_tag="ACGE4H_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8364577.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8501..9109)
                     /locus_tag="ACGE4H_001147"
     CDS             complement(8501..9109)
                     /locus_tag="ACGE4H_001147"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFF8364578.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9194..9664)
                     /locus_tag="ACGE4H_001148"
     CDS             complement(9194..9664)
                     /locus_tag="ACGE4H_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFF8364579.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9712..10626)
                     /locus_tag="ACGE4H_001149"
     CDS             complement(9712..10626)
                     /locus_tag="ACGE4H_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8364580.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10772..11575
                     /locus_tag="ACGE4H_001150"
     CDS             10772..11575
                     /locus_tag="ACGE4H_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFF8364581.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11753..12700
                     /locus_tag="ACGE4H_001151"
     CDS             11753..12700
                     /locus_tag="ACGE4H_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFF8364582.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12768..13061)
                     /locus_tag="ACGE4H_001152"
     CDS             complement(12768..13061)
                     /locus_tag="ACGE4H_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFF8364583.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13089..13754)
                     /locus_tag="ACGE4H_001153"
     CDS             complement(13089..13754)
                     /locus_tag="ACGE4H_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFF8364584.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13894..14526)
                     /locus_tag="ACGE4H_001154"
     CDS             complement(13894..14526)
                     /locus_tag="ACGE4H_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8364585.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14533..15600)
                     /gene="liaS"
                     /locus_tag="ACGE4H_001155"
     CDS             complement(14533..15600)
                     /gene="liaS"
                     /locus_tag="ACGE4H_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFF8364586.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(15597..16328)
                     /gene="liaF"
                     /locus_tag="ACGE4H_001156"
     CDS             complement(15597..16328)
                     /gene="liaF"
                     /locus_tag="ACGE4H_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFF8364587.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(16496..16975)
                     /gene="greA"
                     /locus_tag="ACGE4H_001157"
     CDS             complement(16496..16975)
                     /gene="greA"
                     /locus_tag="ACGE4H_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFF8364588.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17111..18286)
                     /gene="mltG"
                     /locus_tag="ACGE4H_001158"
     CDS             complement(17111..18286)
                     /gene="mltG"
                     /locus_tag="ACGE4H_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFF8364589.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            18451..19611
                     /locus_tag="ACGE4H_001159"
     CDS             18451..19611
                     /locus_tag="ACGE4H_001159"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFF8364590.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         5912 a         3990 c         3166 g         6634 t
ORIGIN      
        1 ttcttttatt ctttttttat ttttgaaaac ttgccatact ttttatttgt tttattttcc
       61 agtatactat acgaaaggaa ggtggctaga ccatgaaagt tgtacaaaca aaagatacaa
      121 tgagcaatat ttacttagat gctgtacgga tcaggcatca agtttttgta gtcgaacagg
      181 gtatgccttt gtctcgagaa atagacaaag atgaagccca ttgtatccac ttcgtcctgt
      241 actcagataa aaaagaaccg caaggaactg tacgtctttt gcctttagaa aacggaaaaa
      301 tgaaattaca gcggatggcg atccttttga agatgctggc atgcctcata tttatatgaa
      361 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
      421 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
      481 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
      541 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcggataat tcatcatttt
      601 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
      661 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
      721 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
      781 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
      841 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
      901 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
      961 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     1021 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     1081 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     1141 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     1201 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     1261 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     1321 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     1381 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     1441 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     1501 tccagaatga ttttgaagag ccgcactgat ataataaacg tcttttggct ttgctttttt
     1561 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     1621 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     1681 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     1741 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     1801 tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca aatgcgatac ttgcaatcgc
     1861 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     1921 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     1981 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     2041 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     2101 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     2161 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     2221 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     2281 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     2341 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     2401 aataatcata ttctatttct tgatctgctt cttgcggaag atcagccatc gcttgttgga
     2461 tcacttcttg agaaaaacct ttcgtcatca atgtttgttg gatttttctc aataattctt
     2521 tttggctttt tccgcgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     2581 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     2641 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     2701 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     2761 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     2821 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     2881 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     2941 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     3001 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     3061 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     3121 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     3181 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     3241 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     3301 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     3361 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     3421 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     3481 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     3541 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     3601 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     3661 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     3721 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     3781 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     3841 aaaacgcaat gatctgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     3901 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     3961 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     4021 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     4081 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     4141 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     4201 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     4261 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     4321 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     4381 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     4441 aaaagttcaa gattgaatat ttacagtctg tcgacatgtt tcctcagact gcacgttgtg
     4501 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     4561 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatacc
     4621 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     4681 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     4741 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     4801 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     4861 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     4921 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     4981 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
     5041 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
     5101 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
     5161 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
     5221 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
     5281 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
     5341 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
     5401 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaac caaatcatat
     5461 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatc
     5521 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
     5581 actactatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
     5641 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
     5701 agaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
     5761 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
     5821 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
     5881 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
     5941 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctacatc atcgcctggc
     6001 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
     6061 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
     6121 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
     6181 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
     6241 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
     6301 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
     6361 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
     6421 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
     6481 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
     6541 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
     6601 ctggtcttgt ttgattgttt caaacgcagg ggtgtagttc gtcgctacat caagcatgtc
     6661 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
     6721 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
     6781 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
     6841 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
     6901 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
     6961 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
     7021 catttcgaca ccaccaaatg cataggcatg atttaattta tctaccgttc cataacctac
     7081 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
     7141 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
     7201 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
     7261 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
     7321 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacaa atacccctaa
     7381 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
     7441 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
     7501 aaaaaacatt tttgtgtaac gattactatt ttttaaaatt tctcaaaatt cttaactatc
     7561 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
     7621 ttttttacgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
     7681 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
     7741 atcattcgat ttcacgaaaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
     7801 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgatttcat
     7861 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
     7921 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
     7981 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
     8041 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
     8101 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
     8161 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
     8221 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
     8281 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
     8341 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
     8401 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
     8461 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
     8521 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
     8581 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
     8641 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
     8701 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
     8761 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
     8821 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
     8881 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
     8941 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
     9001 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
     9061 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
     9121 tcctcaatgt tttctttata atgtctacat tacaaagagt aacaaagaga aggaaattct
     9181 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
     9241 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc taggataaca ataatttgag
     9301 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
     9361 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
     9421 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
     9481 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
     9541 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
     9601 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
     9661 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
     9721 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
     9781 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
     9841 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
     9901 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
     9961 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    10021 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    10081 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    10141 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    10201 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    10261 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    10321 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    10381 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    10441 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    10501 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    10561 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    10621 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    10681 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    10741 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    10801 ctttatacga ttcaaagacc taaaggaggc gagaaatgtc ccattttgga aagccattgt
    10861 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    10921 cttaaataac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    10981 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    11041 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    11101 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    11161 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    11221 tttaaaaaac ttaacaggta agcagctccg ttctttttta agtgaagcat ctataccact
    11281 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    11341 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    11401 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    11461 aacaatcttg atggtctttc ttccttcttt tgacactagc gccttcattg cttttgcagg
    11521 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    11581 aaagacaact cgttatttct acttaaatag acccttttag aggttgtgac atttttttgt
    11641 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    11701 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    11761 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    11821 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    11881 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    11941 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    12001 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    12061 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa
    12121 atggttaggt tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg
    12181 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    12241 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    12301 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    12361 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    12421 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    12481 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    12541 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    12601 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    12661 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    12721 aaaagaaaaa agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta
    12781 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    12841 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    12901 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    12961 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    13021 tcaaaccatt tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc
    13081 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata
    13141 accagtagat tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt
    13201 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    13261 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    13321 tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc aatccgtatc
    13381 cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag tacacgggca
    13441 tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt taccatcaga
    13501 gtaactaaga tgctggcttg gatatcttct ccaatagcga caattacatg atcgaaattt
    13561 cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac agcatgggtg
    13621 gctatattca tatattcatt gactcgatct tcattactgt caatagccag tacttcttgt
    13681 ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc tacaattgca
    13741 aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    13801 tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    13861 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttaaacgc
    13921 atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat tcgacacatg
    13981 cgttttgact gttttcagtg tgataaacaa ttcatccgcg atttcctgat tacttttccc
    14041 ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat gcaatactgg
    14101 atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt ctagtactcg
    14161 ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgctgtag acgtcttcaa
    14221 caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat caataaaact
    14281 tgtgacgata atgattttgg cttttggcca atcttttaag attgcttttg ttgattcgat
    14341 gccatccatt tcttccatga ctagatccat taaaatgaca tccggtcgaa gtgccatcgc
    14401 cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt cttcttggat
    14461 agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta ataaaacttt
    14521 tatcatcttt atctatgcct ccttcatcaa agggaccttg gtctccacgc tcgtaccttg
    14581 ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    14641 gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc cgtcgtcaat
    14701 gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt cattagcctt
    14761 agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    14821 ggaactagtt aattttacat cttctacctc ccattttagc gaaatctgga ttttattctg
    14881 caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta aattgactgg
    14941 acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgccac
    15001 cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt gctccgtcaa
    15061 tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat gaagctcacg
    15121 agctaatcga tgcctttcta attttaaaat ctcttctttg gtttgtccat ctacatattg
    15181 aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt cttttatttt
    15241 agtaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg ggatcggctg
    15301 atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt cttcgatttt
    15361 cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc ctacacctaa
    15421 tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac gcagcaattc
    15481 tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa atagaataat
    15541 aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc ttcccatcat
    15601 attcgtatca cctcgacatc tccaaccaaa gtattggtaa tgatcttcaa tctgcgagga
    15661 ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga aaactgctct
    15721 tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc cactcccagg
    15781 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    15841 ggcaaaagtg tattgcccaa atcgatgata gtatcaccag aaatgatggc aatgttgata
    15901 tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg ctgtctttta
    15961 cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg tttttttcgc
    16021 caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag cccgataaat
    16081 aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    16141 accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata tttgcgcata
    16201 gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt attgtttacg
    16261 atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa acgccaagaa
    16321 ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct atttttttga
    16381 ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat actcaaagag
    16441 aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag tcagactacg
    16501 cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca atggttactt
    16561 catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg gaaatctttc
    16621 cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca tctccatcag
    16681 gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct acgccgtcat
    16741 tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt ccttcaacaa
    16801 atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct ccgaaaccgc
    16861 gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt tcttctaatt
    16921 ctttttctaa tttttcttta ccttctagag tcattggata aactttctct accatgtgta
    16981 acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat atagtccaaa
    17041 aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc aatgactagc
    17101 ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt tcttcatacg
    17161 ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa tagtcatttt
    17221 ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa ggaccaggcc
    17281 catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct tcatacgtca
    17341 ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga agtggcatat
    17401 ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt tcttttacgc
    17461 cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa tttttctgtt
    17521 tgatagctga ataatagttt tgcatgaccg tatttgtttt gttcaccatc tcaatcacaa
    17581 gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag tagccttcca
    17641 aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg aatttttgat
    17701 acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc ttgtctttac
    17761 cagttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct ggaatggcaa
    17821 ttttcccatc tgcaactttt gttggctcag aagtgccgcc ttcttgcagc tgtttgccaa
    17881 tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag ccagttaaat
    17941 ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg tccttctcca
    18001 aaatctcacc aatctgcttg ttagaagagc cactcgggat ctccacttgg actaattggt
    18061 catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg gtaaatccaa
    18121 gaaatccgcc aataattaat aatgtaagag caatcactaa aatgatcttc ctcacaatct
    18181 tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta gctgccttac
    18241 tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt
    18301 actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt gaattatttt
    18361 accaaatatt atgtcaaaat tgtaagtggc aaatactctt tttgaggatt acaatgaaaa
    18421 atagttaaaa atggatagaa aggaattaac atgtcaaaag gaaaacatcg aaaaaaacaa
    18481 aaaatttcta tttcttttct tgtaatttta ttattgtctg tcagcttctt ttcaacaggc
    18541 atcattatag gaggtcaatt aacaaaaata cctcattccc aaacttataa agcggagcca
    18601 acacaactta cttccaattt aacgcccgat gagcagaggc ttgctagcaa aattgatcaa
    18661 ttactacaaa aatcagatta tatcggttcg atttatgttc gcaaaaataa tcgcgtacta
    18721 ttagaaaaag gatatggata tgcaaatctg cgcgtaaaag aatcgaattc accttctatt
    18781 tactatcaaa tcggttcgat tcaaaaagca atgactgctt tactgatact aaaacaaatc
    18841 caagctggta agttaacttt tgacacaaag ttagcagaat tttatcctca gattcctgga
    18901 agtcagcaaa tcacaatcaa aaatatgctt tatatgcgct ctggtcttca tcgaactgct
    18961 tctcccgaca aacctatgtc ggatagtgaa atcattcagt tcgcccttca tcatttgaaa
    19021 cttactgatt atgatacgta tcattatgaa ccattgaatt atacattgct tacagggata
    19081 ttgatcaaac taacgaatca gccttatgaa actcttctaa aaaaagaaat cattcgtcca
    19141 ctccatctag aacatacaga tttttatgac aatgtaaaaa attcgcctca acatgctgta
    19201 tcttaccaga tgtctgcaac tgacgattat tcaaaagcat tgatcgagcc tgaaaccgac
    19261 atccgaaatg aattaggtac aggaaacata agtatgtctg tttatgactt gaacaaattc
    19321 tttaccagtg ttctttctgg cggtatcata ccaaaagaac tacttttctc attgtggcaa
    19381 aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaacga ttatatcctt
    19441 gctcagggga atatcaatcg attccatgca gtagtggcga tgaaaaaaga cactaaagat
    19501 gcaatcgtca tggaaagtaa tatccaatcg gataagaaca tcaaattgcc ggccactgat
    19561 ttaagaaatc agatttatga actgatggaa ggtgttcact tatcaaatta aattttgaca
    19621 taatattagt tgtcatctct gtatagtcaa tttagttagt actctatctg aaaagtagaa
    19681 ataaattaac aaatattatc tg
//
LOCUS       DAXWLB010000043        19403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXWLB010000043 DAXWLB010000000
VERSION     DAXWLB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.043"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(40..636)
                     /locus_tag="ACGE4H_001160"
     CDS             complement(40..636)
                     /locus_tag="ACGE4H_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFF8364591.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            986..1330
                     /locus_tag="ACGE4H_001161"
     CDS             986..1330
                     /locus_tag="ACGE4H_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFF8364592.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1331..1750
                     /locus_tag="ACGE4H_001162"
     CDS             1331..1750
                     /locus_tag="ACGE4H_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFF8364593.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1842..2192)
                     /locus_tag="ACGE4H_001163"
     CDS             complement(1842..2192)
                     /locus_tag="ACGE4H_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364594.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2158..3489)
                     /locus_tag="ACGE4H_001164"
     CDS             complement(2158..3489)
                     /locus_tag="ACGE4H_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFF8364595.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3744..4310
                     /locus_tag="ACGE4H_001165"
     CDS             3744..4310
                     /locus_tag="ACGE4H_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8364596.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4455..4542
                     /locus_tag="ACGE4H_001166"
     tRNA            4455..4542
                     /locus_tag="ACGE4H_001166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4487..4489,aa:Ser,seq:cga)
     gene            4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4H_001167"
     CDS             4878..5852
                     /gene="bsh"
                     /locus_tag="ACGE4H_001167"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFF8364597.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4H_001168"
     CDS             6177..8072
                     /gene="asnB"
                     /locus_tag="ACGE4H_001168"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFF8364598.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8267..8713)
                     /locus_tag="ACGE4H_001169"
     CDS             complement(8267..8713)
                     /locus_tag="ACGE4H_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFF8364599.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4H_001170"
     CDS             8941..9090
                     /gene="rpmG"
                     /locus_tag="ACGE4H_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFF8364600.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9178..9696
                     /locus_tag="ACGE4H_001171"
     CDS             9178..9696
                     /locus_tag="ACGE4H_001171"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8364601.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9773..10729)
                     /locus_tag="ACGE4H_001172"
     CDS             complement(9773..10729)
                     /locus_tag="ACGE4H_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364602.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10895..11701
                     /locus_tag="ACGE4H_001173"
     CDS             10895..11701
                     /locus_tag="ACGE4H_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364603.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11698..12687
                     /locus_tag="ACGE4H_001174"
     CDS             11698..12687
                     /locus_tag="ACGE4H_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8364604.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12684..13688
                     /locus_tag="ACGE4H_001175"
     CDS             12684..13688
                     /locus_tag="ACGE4H_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFF8364605.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13820..14362
                     /locus_tag="ACGE4H_001176"
     CDS             13820..14362
                     /locus_tag="ACGE4H_001176"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFF8364606.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14382..15080
                     /locus_tag="ACGE4H_001177"
     CDS             14382..15080
                     /locus_tag="ACGE4H_001177"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8364607.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15101..15313
                     /locus_tag="ACGE4H_001178"
     CDS             15101..15313
                     /locus_tag="ACGE4H_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFF8364608.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15313..16275
                     /locus_tag="ACGE4H_001179"
     CDS             15313..16275
                     /locus_tag="ACGE4H_001179"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFF8364609.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16293..16688
                     /locus_tag="ACGE4H_001180"
     CDS             16293..16688
                     /locus_tag="ACGE4H_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFF8364610.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16851..17216
                     /locus_tag="ACGE4H_001181"
     CDS             16851..17216
                     /locus_tag="ACGE4H_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFF8364611.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17213..19024
                     /locus_tag="ACGE4H_001182"
     CDS             17213..19024
                     /locus_tag="ACGE4H_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFF8364612.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19086..19253)
                     /locus_tag="ACGE4H_001183"
     CDS             complement(19086..19253)
                     /locus_tag="ACGE4H_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFF8364613.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6281 a         3300 c         3945 g         5877 t
ORIGIN      
        1 taacattgtc aatcaatgac taattttaca aaataaatat tatccaagaa gaccacttag
       61 atattgacct ccataaatca aggccatact atataagaaa atcgacagtg gtttagcaat
      121 caaaatgatt agtgtatatc gtttcactga catgtttgtc aatccagcca tcaaacataa
      181 cgcatcgtca ggagcaactg gaagaaaaat agctagagca aacagcttct caaaacggtt
      241 ttgattatcc agccagccaa tatatttgtt atatgtctta tcgctaatca tggaaagtat
      301 gaatggcttt ccataacgac gccctagcag aaaaatgata atcgaaccaa tgcagattcc
      361 aatatagtta tagataaatc cagcataagg gccaaatatc aggacaccag ctgcagtact
      421 gatccctcca ggaatgattg gaataactac ttgcaggatt tggatgaaga caaaaataat
      481 cggtcctaaa atgatggaat cacctactaa tcctcttaac gcattcaaat ctttgaaaac
      541 acctaaatta ataaaataaa tcgtcagcgc gattgatagg cctaatccga taatggatat
      601 gaaatttatc agttttcgag aggcagcaat attcattatt catcaactcc ttagaggttc
      661 tttgttaact atactttact agaaaagcaa ctaactgcgc cattttttga tatgacagaa
      721 aacaaacaat tttgtaagtt catcataagt ataaaagatt gatctcaaaa aaacatccaa
      781 ctaaagactt atttaataac ttgttttttt taaaataaaa aaagcgttag caactatgct
      841 taaaaagcat atcgactgaa taaactaagc attggacagg ctgaaagact acatttaaaa
      901 tgaaatgcga agaaacaata gaaaataaag ttgccttaga accatagttg aatcacctaa
      961 cagtcgtgaa gggagaataa gagaaatggc agaaaaacaa accgcaggac gagatcaatt
     1021 aggagatttt gcaccaaaat ttgcagaatt aaatgatgat gttttatttg gacaagtatg
     1081 gtccagagaa gaattatctg ctcatcagcg tagtatcatc accatatctg cattaatttc
     1141 aggaggaaat tttgaacaat tgccagctca tctgaagatt ggcaagcaaa atggcattac
     1201 tagtgaagaa atagcagaaa tcattaccca tttagctttt tatgtagaat ggccaaaagc
     1261 ttggtcagct tttagtttag caaaagaaat ctatttagaa gaaaatagcg aagataagaa
     1321 agaggcgtaa atgatgacaa aacacgaaga aatacacaat ggcgtgattt ttccagcagg
     1381 tgaaaaaaac gaagcttacg ctcaagtatt taccgggcaa agctatctga atatgttagt
     1441 gaatgatccc gaagtttctg ttggtgtagg taatgtaact tttgaaccag gttgtcgcaa
     1501 tcactggcat gtccatacag acggctatca aatcttactt gtgaccggag gagaaggctg
     1561 gtatcaggaa gaaggaaagc cagcaagacg attaatggct ggagatgtcg tcgtaacgca
     1621 caaagggatt aaacattggc atggtgcgac aaaagacagt tggtttgaac atttggcgat
     1681 cactgctggt aattcagagt ttttagaacc tgtaacagat gaagtgtatg aaggattgga
     1741 aaatcaataa tgtcaagctt gaacagctcc tgaaagtaga ttttaatcga actttcagga
     1801 gctgcttttt tgattcagtc ttttataaat agaaaatagg gttaataata ggaatgagaa
     1861 gcacgtcgaa agatcttaat attgataatc ggctgggcat acatgatttc cacgtcatca
     1921 atcaaactac ctaacagatg ataatcttcc cgatcatgtc ggggattacc taatagttct
     1981 tgatagtaga tttccatact agggtcccga ggattgttca gcaaatcagc aagtacatct
     2041 aaatcttctg gctgagtttc tgccgtaccc tgtatctcta tgaaaagccc ttcctcagca
     2101 aattctactc caatacgtat ttcagttatt tcatgggaat ataaataagt aagaagctca
     2161 tcgatgattc tttttgataa ttctttttcc aagtgtcttt ttttcctttc tgacagcggt
     2221 aaagttatct agtatcggta caaggatacc tgcaacgaaa ccagttgaaa aaccactgtt
     2281 atatagattt agtcctccgt gcaaagtgct gacgttatgg acaagagcaa tatgtagcac
     2341 accagctatt acaccgtaaa aactgccgta aaaaccgctt atgggcgcaa gacctgtccc
     2401 aaacacagct gcaacaagca tgccagtact ggtgatctca tgaaaagttc caaacaaaga
     2461 agcaataaat attccgacta gtatcggaaa actgtttttt aaatgacaac caaaagcact
     2521 gaaacccacc gcagataaaa tagcaccgat gacaggtcca tttaactgac cgccaaccag
     2581 caaaacataa gaagacagca tcagaccaac taagcccatg ttgatcaaag tagcaccaat
     2641 accagaatta gcaatgaaat cagttgtcaa ttttccagat gaatcgaaaa tttctcgaat
     2701 ccccgaaagc cgaaaggaat tataatagaa accaattccg actgtcagcg aaaaaaagcc
     2761 aaaaataatc caaatgagag gaaagtgata ttcattgaaa accaatgtat tttcgacgat
     2821 tgaatagctg aataacctca aaacagcagt aaaagtcatc gcaatcaacc cagaggtgaa
     2881 tccaatatta taaagtgtga atccacgatg gaaaactaaa gtttgagcag ctaagggcgg
     2941 aagaagtata ccaattgcta tgccagctaa attccccaat aaaataccta tcagtaaagg
     3001 aaaacgcata ccaaaagtga tataactaac gaccggtgat aatacgctac caaacagtcc
     3061 gatcatgatg tactgagaaa aaggtttatt gacaaataca gcgtatagat acacacctaa
     3121 aatgattgag atagaattat aaaggttttt gccaaaaaaa gagaaaccgg taactgttag
     3181 aatcgaagca aataagggac cgtttaaaat caccttgtgc cgataagcta aaagcacaga
     3241 aagtaaggtc aaaatcccta catttatgaa tgcgcttccg aaaacaccta aagcaaaata
     3301 atctgtcaaa agattgcttg gcgaggtaag aatagttagc aatccactcc aattatcagt
     3361 aggagtatta gaaagcaagc caataaaaag aaaaaaaaca cctgtaaaac caagataaaa
     3421 atactttctt ttctgtgaaa cgattcgttt ttgaggatga atataaataa cgtcagtttc
     3481 gttttccatt acacaccttc tttttcattt tctttaacta taacttgaaa tgaaagattt
     3541 ggacaactat catttaggtc aaaactaaaa atgtaaggga acccattgaa tttaaccaaa
     3601 tttttttaat tttgcaaaaa aaagttaaag aagactgaaa aaaatttaaa atataacgtt
     3661 ttgagtaaaa tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc
     3721 agtaaagaag tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa
     3781 aagctgctta tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg
     3841 ctaaaaagat ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt
     3901 taaaaaatac gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg
     3961 aacgtactgg tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc
     4021 atcatagtct ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg
     4081 atttttcata caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat
     4141 cggatgaata tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt
     4201 cattgatgtc atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac
     4261 ggacaattga ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca
     4321 gtatgaattt gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa
     4381 aaagcgattt ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta
     4441 aaaatagtac ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa
     4501 ccggctaata ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg
     4561 ttgtgaagaa gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct
     4621 attttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg
     4681 gaataagggg ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac
     4741 aatatttttt gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt
     4801 tggccttttt tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg
     4861 ttttaggagg gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg
     4921 gaaggaattt tgattatgag atgtcttata atgaagtagt cactattact ccaagaaatt
     4981 atcgtttcga ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag
     5041 cagctggggt atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta
     5101 tggcaggact gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata
     5161 acgttgcacc atttgagttt attccatgga tactagggca atgctcaacc atagacgaag
     5221 ctaaagaatt attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct
     5281 tgtcaccttt acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga
     5341 aagatggact tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg
     5401 attatcaatt atttaatctg aacaattatc gctcgctatc aaatggaaca ccagaaaatc
     5461 atttttcaaa ccagatcagc ttgaatgttt atagtcgtgg gatgggagga ttgggcttac
     5521 ctggagatct ctcttctgtt tctcgatttg taaaagccac atttactaaa atgaatgctg
     5581 catcaggtga ttcagaatct gaaagtatca gtcaattttt tcatatacta ggctcagtcg
     5641 agcaacaaaa aggagtatgc gatactggag aaggaaaata cgaatatacg atttattctt
     5701 cttgctgtaa cgttgataaa gggatttatt attatcgtac gtatgaagac agtcaaatta
     5761 ctgctgtaaa tataaataaa gaagacttgg atcgtcatga attggtcagt tatccagtca
     5821 taaaagagca acagataaac tatgtcaatt aattaatgaa tagatccgtt tggtatcata
     5881 ttttctgata cagtcataat tagacacgat ctttaggtcg tgtctttttt tgctgttttt
     5941 tttggaaata gcatccacta atattttttt aacacgaaga gaataaggta gcctcaaaaa
     6001 agaaaacatg gtaaattaga tattgggact ttattttctg taaaaacaga aactttcatc
     6061 ttttgttttc acgtgacgaa attgtaagag agaaaatatt cgtccgctat ttattctatg
     6121 ataagatgca tatgaatgaa ataaacagta attttatatt aggatgtgaa agttaaatgt
     6181 gcggtattgt aggttttgtt aacgacaagg acaacaaaaa aacaattatt aatacaatga
     6241 tggatcgaat cgttcaccgt ggacctaata gttcaggaga atatatcgat aaacatgttg
     6301 ctttaggatt cagaagatta agtattattg acttagaggg cggtactcag cctatttaca
     6361 atgaagatcg gacaaagatc attattttta atggcgaaat ctataattac cagcctttga
     6421 gagaagaatt gattgcagct ggccacgttt ttcaaacaca tgcagataca gaagtccttt
     6481 tgcatggtta cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga atgtttgctt
     6541 ttgctatttg ggataatgaa aagaatgagt tattcggtgc aagagaccac tttgggatca
     6601 aaccatatta ttatgcagaa atgaacggaa catttatgtt tggttcagaa atcaaaagtt
     6661 ttctgcctca tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa ccttatatga
     6721 ctttccaata ttctccatta aacggcgaaa cattcttcaa agatgtctat cgtttgcctg
     6781 aaggccatta ttatacgtat aaagatggaa aactagacat tcagcaatat tgggatgcag
     6841 attttgaaac aaaagaaacg cattcccgac aagaatggat cgaaaaaatc gacgaaaccg
     6901 ttcaagcttc tattgaagca catacggtca gtgatgtaga agttggttct ttcttatcca
     6961 gcggggttga ttccagttat gtaacctctg tattgaaacc agatcattct ttctcaatcg
     7021 ggtttgatga taaaacctat aatgaagcga ttgaagccag aaaactaacc gaactattgg
     7081 acttagataa tacagcagca gtgatcgacg gagacatgtc gttcaaagca ttccctttga
     7141 tccagtatca cttagatgaa cctgattcaa atccttcttg cgtacctttg tactttttag
     7201 caaatcttgc ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg gatgaactgt
     7261 ttgcgggata ccaaacgtat ggattccata cgaattcaaa attcatccgt gtgattgctc
     7321 aaggattgaa aaaattacca aaaggaacac gctataattt aggacgtaaa atcggaaaaa
     7381 tgaaaaattt ccatggacgg atccatttat atgaatcact tgctcctgca aaagaatatt
     7441 ttatcggtca tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta acaccaaaat
     7501 atcaaacagc accatctgtg gatgaaatca tgactgttca ttacgagaaa acagaaggaa
     7561 tcaaagatga agtcaataag atgcaatatg tcgatcttca tcaatggatg ccaaaagata
     7621 tcttgttaaa ggcagataaa ctttctatgg caagttcttt ggaagtccga gtacctttat
     7681 tggatatcga agtcatgaaa ttggctcaac aaataccaag taagttttta ttgaatcaaa
     7741 acaacacaaa agatattttc agacaagctg cgaacaaaca tcttccggaa gaatggtcga
     7801 atcgtgtgaa attaggattc cctgtgccaa tcaaagcttg gttgaaagaa gaacacggct
     7861 acgaacaagt caaagcatta ttcgaagcag attttgcaaa agaatttttt gatcaagaaa
     7921 aaatcatgaa gttgctagat gaccatcacg aaggacgtaa agaagagcaa agaaaaatct
     7981 ggacaatctt cagtttcttg acatggtata aagtctactt tgtagatgaa acgattccgc
     8041 aagcagaagc aatcgactat gtgactgttt gatgaaaatc aaatatcaat aaaaaagagg
     8101 ttttctccta ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca gctcttcccg
     8161 tttcatagat attatgactt atggttcgga gttggcagag gaacaccatt gtttcgaaca
     8221 taagggggtt gtgacacgat tttcgtcaca acccctttac atatgttcaa taatcgattt
     8281 ctttttcttt tgtcccatct tttttataaa tgaacaaagt tgattctttg ttttgtgcta
     8341 cttgctttcc tttttcgatt gcttcttttt ttgtatcaaa atggttgctt gccttttttg
     8401 cccctttaga tttcacgatc cattgttctt cctcatattc tacgatcaca tctgaatcaa
     8461 gcagttttcc agcacgagga ttcgtatcgt gttcatccgt cttgcttgga tttttttctt
     8521 tttcgaaagt tcgtttttct gattcactag catcttcata ccattttttt gcttgatcaa
     8581 tggcaattgg gatcgcacgg ttatcagggt acccttcatc taataatgca tttgcgatgt
     8641 cgattgcttt ttttcttgtc aatttatcta agtttttcat tgatgcagga aagtccttca
     8701 tgttccatgg catattagta tcccccttta ttaaaaaaca taatctatat aaacaagtgt
     8761 aagggtattt atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt tgtatttatc
     8821 gtcaagtagt tttacttaac aaaatcgtga aaaacactag caatctagaa aaaatcatgg
     8881 taagataatc aagtctgaga gtcatcttag aggattcttg taaaaatggg agggaaaaac
     8941 atgcgcgtaa acatcacttt agagtgtact tcttgtaaag aacgtaacta tctaacaagc
     9001 aaaaacaaac gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc acgcgaacgc
     9061 aaagttactt tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt ccttgctggt
     9121 caaagaattg agactttttt tatctcaaaa catctaaaat cataaggagc ggataatatg
     9181 tttatttcca tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg tttgttacca
     9241 tggcgattgc caaatgatat gcgtttcttt cgcgaacata caatggatcg aattttagtg
     9301 atgggaagaa agacctatga aggaatgggg gatctttcac ttccttatcg ccatatcatc
     9361 gtgttgacaa cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat gcattcgatc
     9421 gatgaacttt tgtcgtatag tcaaacgatc tcagaagata tctatgtttc gggtggtagt
     9481 cggatttttc aagaacttct tccttacact ggtcggatct ggcgaacatt gatcgatagc
     9541 acgtttgaag gcgatacgta tattgggaat attgattttt cagacttcgc ccttatagag
     9601 gagtatgagg ggataacgga tcaagaaaat ctatatgccc atcgttttca aaaatgggaa
     9661 agagtaaaaa atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta gtttgttaat
     9721 aagaaaaaaa gcatctcacg actaatcgtg aagattcttt ttttagttaa aatcaattta
     9781 gtttttcttt gatatcattg accatttcct gactggcaat aggtccgtta tatagccaac
     9841 tagattcagg tccgaattgc agtacatgat tgtctgtcac tgctttaaga ttcttccagc
     9901 ttggttcatt gaacatggca gcatctttgt cactgttcac aaggatgatg tagtcagcat
     9961 ctaattcagc tagcttttca gaggaaacag cagaccagtc agaagtggct tttttggaga
    10021 cctcttccac tagatcagga acaccaaatt ccagatcgtc gtatacgatt cttccacttg
    10081 accgattttt gctgaccata aaggctgagt tgttggtcac ccacaacact gcagctgtct
    10141 tgttctcgat tttatctgct agttcttctc gagttttctt gatggatttt tgataatcgg
    10201 aaatgatttt ttctgcctct ttttcttttg ccaaagcttt gccgacatct tttagttgtt
    10261 cttcccatgt gacatctgtt ccgtttttta caacataagt tggcgcgatt ttgctatatt
    10321 gttcgtattt cccgccttcg acagtagcgg aagaagaaat caacagcaaa tcaggctcaa
    10381 agcttaacac tttctcataa ggaaggtcat aagaaatcgt tgggacatcc tttaaatcat
    10441 cttgcagata atcttgaatg gaaccactgc caacagtcca ctgggcgata ggtttttccc
    10501 ctagtgcaat cagatagtct tctaagtaac tgccgatgat ccgtttgggc gcgtttggaa
    10561 tctctacttt atgtccaagt gtatctgtga ctgtatgagt actattggat gaagtcgtcg
    10621 aattggcagt tgtagctgtt tctttatctg aagaggggga gcaagcgctc agtactccag
    10681 ccagcagcaa tatgctagat aatgtgaata cttttgtttt taatttcatt tctttcctcc
    10741 taacataatt gagaatcttt ctcattggtt attattcctg atatttcgaa cgctgtcaac
    10801 cgcttcattt ggctatagat atgaaagtag cgcttgacga aatgttgaaa cagcgtcata
    10861 attgagaatg attttcagcg aaaggatggg tgaaatgacg agattaagag cagctgacat
    10921 tgcagccggc tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga ttcctgaagg
    10981 gaaaatcact agtttgattg ggccaaatgg aagtggaaaa tctactttat tgaaaagttt
    11041 tacccgtata ctacctgtga aatcaggaaa gatcattgtc aataactctg atatctctac
    11101 atacagttca aaagaacttg caaaaaaaat tacattatta gcacaaacta gtgaacaccc
    11161 tacaggtatg acagtagaag aagtagtatc ttacggacga tacccttatc aaaaactgtt
    11221 cagtggcatg gacacgcaag atcatgaagc aatcgagtgg gcgttggatg ctacaggtct
    11281 agctggattt cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc agcgtgtctg
    11341 gatcgctatg gcgcttgctc aggaaacaga tatcttgatc ttggatgaac cgacaacatt
    11401 tttagatccg gcacaccaat tagagatcct acagttattg actgaaatca atcaaatgaa
    11461 acaaacaacg atcttgatgt cgattcatga cattaatcat gcttcacgtt tttcagatta
    11521 tttattagca atgaaaaacg gtaagttgat cacagaaggt acacctgaag aagtgatcac
    11581 aaaagagtgt atgaaaaaaa tatttgagat tgatgcaacg attgtcacgc ttccaagaac
    11641 aaataaacca gtttttctaa cctatgattt gttgccaaaa gagcaggagg atcaattatg
    11701 aggactatga agaaaaatag actcctgctt ttggtctgtt ttgttctttt gattattttt
    11761 tttgttgctc tttcaaatgg cgcagcgact gtgggaaaag cagatttttg gcaggcgttg
    11821 tttgattttg atagatcaaa acaaagtcac caaatcatac gaaatattcg tttgcctcgt
    11881 gtggcgggag catttcttgt agggagttgt tttgcattga gcggtgcatt gatgcaagga
    11941 gtgacaagaa atcctttagc agattcaggc ttactaggaa tcaacgcggg tgcttcgttg
    12001 gcaatggccc ttagttttgc tttttggcct aaagcttctg atttttctgt gtttctaatg
    12061 tcattggcag gttcttttgc tgtaactttg ctcgtatttg gttttttgca gttttcgaaa
    12121 atgggcatga atcctttaca gctgatttta gctggcgtag ctattagttc tttttttact
    12181 gctatcagcc aagcacttac ccaattcttt aatctgcagc aggatcttgc tttttggttt
    12241 gtaggcggag ctgccaatat cacatggaaa caattagcag tattgtttcc tttatatata
    12301 cttgctgttt tggtttcttt ttcattagga aaagcaatat ctttgattca tttgagtgaa
    12361 acacatgcat tagctcttgg aagtcatcct aaacgaacgc ggttgattgt tttctgtcta
    12421 gttgcagtgt tagcagccat tgccgtttcg ttagtcggac ctgtttcatt tattgggctg
    12481 ttggttccgc cagttgttcg cggaatggtt ggaacggact atcgttatgt tctacctgtc
    12541 tcttttttag ttggcggtgg attggttatg ttggcggatt ttacagcgag gatgatcaat
    12601 cccccttttg aaacaccttt tggattagtc attgcattga ttggcgttcc gtttttactg
    12661 atacaagtaa ggagggaaca acggtgaaaa aaatatattg gacgttctat ttcttgcttt
    12721 ttgtacttgt catcctgtct ataggtagtt taatgattgg tacgcctttt atttacctgg
    12781 atcagctttt tgccacgttc atagggaaag aatcatcttc tttggcgctg attgttagag
    12841 aatttcgagt tcctcgatta ctgatcagtt tattagcagg agcttgtctt ggcgtttctg
    12901 gttatatctt gcaaggagta acaagaaatg aattggcaga ttctagtata ttgggcatta
    12961 atgccggagc aggattttta gtgatgcttt atttaggttt cttttcgcac tattcgctgc
    13021 cgtttctatt gctagttgta gcctttctag gtggtgtttt agctgcttgc tgtgtctatc
    13081 ttgcggccta tgatcgaaat ggctttttgg gtatgaatcg aatcttattg tccggtattg
    13141 ccgttaatgc cggcctttct gcattaacat tgctatgcac gatccaatta tctaaggaaa
    13201 attatggatt tgtgaatgca tggttagcgg gatcaatctg gggtgcgagc tggccttatg
    13261 tttgggcatt gcttccatgg gcggttatct taattccgct aggaggactt tatgcacaga
    13321 ggattggatt gctatcattt ggtcaagaac gcgcgcaagg attaggcctt aacgtggcta
    13381 aaagtcaaaa aatccttttg ttgttagctg ttgctttggc aagcggtagt gtagcggtga
    13441 caggtagttt atcttttgta ggactactag ctcctcatgc tgcgaagcta gtcgtacgta
    13501 aagaaaataa ctggaccttt gttctgagcg gtttgttcgg ttccattttt ctgcttagtg
    13561 cagatatggt tgctagagtg attctaccaa gtggggaagt acctacagga attttgattg
    13621 ccctttttgg tgcgccatat tttctgtatg cactttttat cagacaaaaa cacgcgctga
    13681 acagttgaaa tttattaaaa aaggaaggcc aatcatttct aggtcttctt tttattctat
    13741 ttatgcattt atttgaaagt tcgattgcta tttttctttt tttcctatac cattttatga
    13801 gagaaggaag ggtgagtttt tggaccaatt gaaagtaaga ttacgccgga aaggaatcga
    13861 aaagctgaaa aatctggcca aaagtggcaa gaaaaaggaa aaagaacagc gaatctataa
    13921 acagctattc gccagcgagt actggaagtc agcaaaagtg atagggacga ctttatcgaa
    13981 tgaatttgaa ctggatacac agccggttat ccaacgagca gaaaaagaac aaaagaaaat
    14041 agttgtacct aaaactttgc cgaaacgtca gatggcgttt tatgaaatgg atgaacagtc
    14101 cgttcttgta cgttcgagtt ttggtgttct tgagccacaa tcagagaagc tctatttgcc
    14161 ggagatgatc gatctcctca ttgtaccagg tattatctac aatagagccg gttatcgaat
    14221 cggttttggc ggtggttatt acgaccggta tttaaataat tttcataaaa aaacatgtag
    14281 ccttgttttt cgtgaacaat tgaatgaaca atggcaaccg gagagctttg atcagaagat
    14341 ccaacaattg tttattgact aaaatgaaat gggaggtttt catgaattat caacagcaaa
    14401 taaaattgcg tgcatggtta agacgaccgt tatggaccta tgctttttta gggatacaga
    14461 cagcggtctt cctcatgatg gaattatttc cgcgacttga gatcccttat tatgccggaa
    14521 tgtatggtcc ttatcttgtt cacttcaatg aatggtggcg tttagtgaca ccaatcttca
    14581 tccattttgg actgatgcat ttcgtaatga actcactgat actgtatttt atggggcagc
    14641 aaatcgaggc aatctatggc cattggcgtt ttttcttgat ttatatgttc agcgggataa
    14701 tgggaaatac agcaagcttt gcttttaacg aagcgaatgt tctgtctggt ggtgcgagta
    14761 cttcgatatt cggtttgttc ggtgcgttgt ttatcttagg gtttcatttt aaacataacc
    14821 cagcgatcca gcagctagtc agacattttc ttttatttat tgtgttgaca tttgtttttg
    14881 gtcttctgga tacctctgta gatgtttggg gacatgtcgg tgggctaatt ggcggattag
    14941 tgttagggaa tgttttgggt ctaccgaaac aacagacaag ctattcgata caccaaagga
    15001 ttctttctac actagttttt gtatttcttt ttgtaatatg tattttactg ggtttaaaaa
    15061 aatatggttt acttgtataa tatgaaggaa gtgcttaaag atggagagct tatatgatgt
    15121 acagcagctg ttcaaaaaat ttggcatctt tatctatgtg ggtgcacgca tctatgacat
    15181 tgaattaatg actatcgaat tgcgtaatct gtatgatagc cgtttgattg atcatgatac
    15241 atacatgaca gcgtggcgca tattaaaaag agagcaccga attgaagaaa gccgaaagaa
    15301 aggaaatttg taatggacaa gaagattatt gggattgatt taggtggaac aacagcaaaa
    15361 tttgcaattt taacaccaga aggagagatt caacaaaaat ggagcatcga taccaatatt
    15421 ttggatgatg gtaagcatat cgttcctgaa atcattgaat caatcaacca tcgtttgaat
    15481 ttatataata tgaaagccga agactttatc ggaatcggca tgggtacccc aggaagcgtg
    15541 gacagcgaag ctggaacggt tattggtgcg tataacttaa actggacaga agttcaattt
    15601 gtgaaaaaac tgatcgaggc aggaacagga atcaaattcg ctatcgataa tgatgcgaat
    15661 gttgctgctt taggtgaacg atggaaaggt gctggagaaa acgatccaga cgttgtattc
    15721 gttactctag gaacaggtgt tggcggtggt atcgttgctg gcggaaattt gattcacggc
    15781 gtgagcggag caggcggaga gatcggccat atcactgtgg atcctgaagg gttcgaatgt
    15841 acttgcggta aacgcggatg tttagaaact gtgtctagcg ctactggtgt ggtacgtcta
    15901 ggtcgttact tggcagaaga atacgcagga gattcaaaac tgaaagcaat gttagacaac
    15961 ggtgaagaag taacaagtaa agatatcttt gaaatggctc aagaagacga tccgtttgca
    16021 ttgatggttg ttgatcgcgt atgcttctac ttaggtttag cttgcgccaa cttggggaat
    16081 acattgaatc cgtcaagtat tgtgttaggt ggaggagtat cggcagcagg agaattttta
    16141 cgtagccgtg tagagaaata ttttaaagaa tttactttcc cgcaagtaag agaatcaaca
    16201 aaaatcaaat tagcagagct tggtaatgaa gctggtgtca ttggtgctgc ctcattagcc
    16261 ttgaaatttg cttaaaaaaa cggaggcatt acatggtttt atgggtaatt aatatcattt
    16321 tgctattgat tgtgcttgca atcatctttt gggaagtcta tctacgagta atggccaagc
    16381 gttcagcaac gactttgaca gaagaagaat ttcgagaagg aatgagaaaa gcacaagtaa
    16441 tcgatgttcg cgaaaaagat gtgtttgatg ctgggcatat tttaggtgca agaaacatcc
    16501 cttacactgt tttgaaagat tcattaggat ctattcgaaa agaccagcct gtctatattt
    16561 acgatcaaaa gaaaagcttg agcatccgta cagcaaataa attacgtaaa gcaggttatc
    16621 aagagattta tttacttaag ggtggatatg acggatggtc tggaaaaatc aaaagtaaaa
    16681 aagtataaaa cagatgattg tatgacagct ttaaaaaccg aatcaatcat tagattttga
    16741 ccaagatcac atgaacggcg caatggatag ttgtgccgtt catttttttt attttgtgct
    16801 attatctaca tatataagct aagcgaaaag gcagaaagaa ggaaacaact atgatcaaat
    16861 taaaacgtgt ttaccttgat gctgaaaaag aggatggcta tagaatactc gtggataggt
    16921 tctggccacg aggaatttca aaagaaaaag cgcagttaga tctgtggttg aaagaaattg
    16981 cacctagtaa tgaagcacgc aaaacttttg accatgtacc agaacgattt gcttcatttg
    17041 caaagaaata ccagcaagaa ttgattttcg aagaagaaaa gaagcaagct atttttaaac
    17101 tcagtcaact tatgaaagaa catccagtca ttacttttgt ttatggtgca aaaaatgaac
    17161 atcagaacca tgctgtttta ttgaaaaaat ttatcgaaat ggaggcatgc aaatgaaaat
    17221 agggatcatt ggtgccggac ctagaggatt aagtatagca gaaaggattc tccataataa
    17281 tcaggacaga cattctcttg aactttttct tttcgatccc aatggtcctg gaggtcgagt
    17341 ttggcggttg aatcagccac cggagttatt aatgaattct gtttctcaac aagtgacgct
    17401 ttttacagat gaaacattgt ccagtggagg attggtaaca cagggaccca acttgtttca
    17461 atggagtaaa ggacctgcta cagattatat cgaacagacg gtaaccaaaa ataagcaact
    17521 aattatagaa gaaatcaatc gtctggaaaa agatcagcaa tctactcgat gtttatacgg
    17581 actctatcaa cgctggtttt tctcaaagtt gcaagaagaa tttccagctt ctattcagtt
    17641 tgtttattct ttagtaaaaa aagtcagcaa aagaaaagat ggttatcttt tacagacaga
    17701 tcagcaaagc tattctatcg atcagcttgt tttagcaagc ggtcactggg aaaatgatga
    17761 gacagaagaa gaaagagtgt gggtagacta cgcaaagaaa aatgatttac tgtatcagct
    17821 tccttctaat cctgcagatg tcgatctttc gaagataccg cctaaagaaa atgtcttttt
    17881 aagaggtcta ggcttaagtt tttttgatta tgtcggattg tttacagtat caagagaagg
    17941 gcgcttcgag aacgaaaaag gaaaacttgt ctatcatcca tcaggcaagg aaccgattgt
    18001 atacagtgga tcaagaaggg ggctccctta ttaccctcga ggaagaaacc aaaagcaagg
    18061 cggatcaatg gcatggccac ggttactaac aaaagaaaac ctggagcaat ggcatcagtc
    18121 gggatttttg actggcgaaa cattttttga ctatctgaaa aaagacgttg agttgtttta
    18181 ttacaagaaa atcatcgaag aatatcattt gccgattcca aagaaaaatt ttgagcatga
    18241 ttttctctcc ctctcttcag aggagtgtct cggaaagcat ccagcactaa tcccttatag
    18301 atgggattgg tcgtatttag aaattcctct tacctatcag gatgagtcct ttgcagaagc
    18361 tagccgagca tttattgaag ggcagataaa agaagcggaa aaaggcaatt gcacaggagc
    18421 atttgcttca acatttgatg cattaaaaga ctggcgtgat ccaatccggc aggctattga
    18481 atggcacgtt ttttctgcaa aggaatacaa agaaatacta tggggatggt ttactcatct
    18541 aaatagcttt ttaacgatcg gtccgccagt cattcggacg aaagaattag cagcactgat
    18601 agatgcgggt atttttcatt tgttcgaacc gccatttgat attcagcaaa agcacaaccg
    18661 ctatgttatt tctctacaag ataaagaaat cagcagtcgc tatttgattg aagctcgatt
    18721 gccacaaaca agcttttctc gttcaagtaa tcctgtcata aaaaatttga aggaaaacaa
    18781 acttgttcgc cctttttcat tttcagataa tacaggaagt tatcattcag aagctataga
    18841 tgttgagcga ggtacgaatc gcgtgattga tgcttcaggg gaaatccagt ctgactttta
    18901 ttgctatgga atacctgttg aaggtgtgga ttggctaact gcagcagtag ctagacctta
    18961 tacagatcca tggaatctga gacaggcaga taaaatagca caacttatat tgactgacaa
    19021 ataaaaatac gcatgatgac ttatctgttc tttcacaagt cttcatgcgt gtttttaaat
    19081 aattattaac gagaaacgcg tttgcgcatt gcttttttac ggttttcttc gatcattgct
    19141 tctttttcgt caattggatc gatcactgtt tttttcacag aagcgaccaa accagcaacg
    19201 gcagcggctg ttgccagact tcctactaat attccagata caaatttttt cattgaatcc
    19261 acctcattta ttatttgtta tatctattat ggaacaaaag agaaaaaaag gctagtttta
    19321 cgtttgtttg taaacctttg caaaaaatcc ttgaaaatgt ttactaaatg ttttattata
    19381 aatttaccat atgaatttac gaa
//
LOCUS       DAXWLB010000044        19356 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXWLB010000044 DAXWLB010000000
VERSION     DAXWLB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.044"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(201..1226)
                     /locus_tag="ACGE4H_001184"
     CDS             complement(201..1226)
                     /locus_tag="ACGE4H_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8364614.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1501..3186
                     /locus_tag="ACGE4H_001185"
     CDS             1501..3186
                     /locus_tag="ACGE4H_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFF8364615.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTNKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3200..4483
                     /locus_tag="ACGE4H_001186"
     CDS             3200..4483
                     /locus_tag="ACGE4H_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364616.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4553..5422
                     /locus_tag="ACGE4H_001187"
     CDS             4553..5422
                     /locus_tag="ACGE4H_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364617.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5419..6255
                     /locus_tag="ACGE4H_001188"
     CDS             5419..6255
                     /locus_tag="ACGE4H_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364618.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6281..7348
                     /locus_tag="ACGE4H_001189"
     CDS             6281..7348
                     /locus_tag="ACGE4H_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFF8364619.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7345..8172
                     /locus_tag="ACGE4H_001190"
     CDS             7345..8172
                     /locus_tag="ACGE4H_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8364620.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8217..9242
                     /locus_tag="ACGE4H_001191"
     CDS             8217..9242
                     /locus_tag="ACGE4H_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8364621.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9239..11422
                     /locus_tag="ACGE4H_001192"
     CDS             9239..11422
                     /locus_tag="ACGE4H_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFF8364622.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11422..12550
                     /locus_tag="ACGE4H_001193"
                     /pseudo
     CDS             11422..12550
                     /locus_tag="ACGE4H_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            12547..13224
                     /gene="pgmB"
                     /locus_tag="ACGE4H_001194"
     CDS             12547..13224
                     /gene="pgmB"
                     /locus_tag="ACGE4H_001194"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFF8364623.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13308..13835
                     /gene="msrA"
                     /locus_tag="ACGE4H_001195"
     CDS             13308..13835
                     /gene="msrA"
                     /locus_tag="ACGE4H_001195"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8364624.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14036..14722)
                     /locus_tag="ACGE4H_001196"
     CDS             complement(14036..14722)
                     /locus_tag="ACGE4H_001196"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFF8364625.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14802..16340
                     /locus_tag="ACGE4H_001197"
     CDS             14802..16340
                     /locus_tag="ACGE4H_001197"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFF8364626.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16460..16927
                     /locus_tag="ACGE4H_001198"
     CDS             16460..16927
                     /locus_tag="ACGE4H_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFF8364627.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17461..17889
                     /gene="rplM"
                     /locus_tag="ACGE4H_001199"
     CDS             17461..17889
                     /gene="rplM"
                     /locus_tag="ACGE4H_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFF8364628.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17905..18297
                     /gene="rpsI"
                     /locus_tag="ACGE4H_001200"
     CDS             17905..18297
                     /gene="rpsI"
                     /locus_tag="ACGE4H_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFF8364629.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18385..>19356)
                     /locus_tag="ACGE4H_001201"
     CDS             complement(18385..>19356)
                     /locus_tag="ACGE4H_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8364630.1"
                     /translation="LGQTIRSNRPPSDITISNTKDIFRLHILPMFGEYAMNYLNINTE
                     IISDELTKKSKEYANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEE
                     KRIQEGKQALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWK
                     YIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKI
                     KQTSDQYLFTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIAR
                     QGGADMNQISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6237 a         3509 c         4115 g         5495 t
ORIGIN      
        1 gtggttaaaa atagtttttt cttccctgtg gaaaaaatag tttaacacaa acattatagt
       61 tatccacagc tatctaaaac agatggaaaa taagaaaaat tcgctaaaat accgggaggg
      121 ggtaacgagg gatgagtgat tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc
      181 tgttctttta taattctgtt ctacatttct tgttgttgga tcgaatctgt gagagtaatc
      241 tcggttgaaa gaatgattct tttttcttca gggtattggt ggtggattag ttggaataaa
      301 agttctgtgg ctttgattcc taactgattt gtcggaatgt ctatccgagt caactgtggc
      361 gaaaataggg aggacaataa gggatcactg aatcctgcga tcgaaagatc atcaggtatc
      421 tgctttccag attgttctgc cactttgtaa atactcaatg attttaatgc atcggtagaa
      481 aaaatcgcag ttggtctttc tcgtagcttc attaaatcat tcattgctgc catgctatct
      541 tctaagcgaa agccgccgtc tataatccat tcattacgga tatctaggtg gtgatcaaat
      601 aagcattgac ggtaaccatt gagacgatca ttggatacat aaacttctgt cggagcatgg
      661 agacagccga tatgacggtg tccattcttg atcagtgttt ccaccaaatc gtaactatcc
      721 tggaaattat ccgtgtccac tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt
      781 accacaatag gaatattgta ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag
      841 cttaaaataa taattcctcg gatcattttt tcttggatct ttttcgcgca tttttctagc
      901 tcttcttctg gtgtttttgc cgtttgtaga atcaagtcga agttattttg ttcagaaacg
      961 gcagaaatgg attgaataac atctgaaaaa aaaggattgc cataggtcgt atgagaagta
     1021 cgatgagata atgctagaat cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg
     1081 ctcggttcat aattcaattc tttgattgct tgaagtacac ttgcttttgc tttatcagag
     1141 atatttggct ggttatttaa tactctggag acagttgatt ttgagacatt agcttttttc
     1201 gcaatgtcat aaatagtagg tttcaatttt atatgcacct cctttttggg agcgttttag
     1261 aataattcca tagtacaata gacattcttt tttgtctatg attttgaaaa tttaggaata
     1321 gcttgcatga tcatgagaga ccatattttt ttaagaagct attaattatt gatacataag
     1381 ctttcttatt tgtatttttg tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa
     1441 gcgttttcca taatatgatc aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg
     1501 atgtatacaa aacttttaca agcgatttat cgcgactcag atttgtctga gaaaaccgcc
     1561 tctatgattg aacaaaaagt cagtgtttat cagcagaaga aaggccaggc aggcaaactg
     1621 cattgggacg aagaagatat tttcttgatt acttatgggg atcaattttt tgaagagcag
     1681 caaccaactt tgcgtacatt taagaaattt tatcaaaagt atttggcaga tacatttaga
     1741 gttgtacatt tcttgccatt tttcccatat acatctgatg atggtttctc cgttgtaaat
     1801 tatcaggcaa tcaacgaccg tttgggcaca tgggaagata tagaagaaat gagaagcgaa
     1861 acacggttaa tgttcgactt tgtctgcaat catatgtctg ccgaaagtac ttggtttcag
     1921 gaatatttaa acttgaatga tacgtacaaa gattttttta ttgatttgcc actggataca
     1981 gatacaagca gcgtgactag accaaggacc agcccgttac ttacaacgtt cattgacaaa
     2041 aacgggaaag aaaaacacgt atggacgact tttagcgaag atcaagtaga cttgaatttt
     2101 aaaaatccag aagttttatt gaaaatgatc gatattcttt tgttttactt agaaaaaggc
     2161 gcttcattta ttcgcttgga cgctgtggga tttttatgga aagaactagg aacttcttgt
     2221 atacacctgc cagaaacgca taaaatcatc caattattcc gtgcaatcgc tgatgaagca
     2281 gctccaggaa cggtattgat cactgagaca aatgtgccac acaaagataa tatcagttat
     2341 tttgggaatg gcgagaatga agcacagatg gtctatcagt tttcgctacc acctttgacc
     2401 cttcaagcga ttcgttcggg taatacaaat aagttgaaag aatggttaaa aaccatcgat
     2461 ttcgcatcga aaaaaacaac tttttttaat tttttagcct cccatgacgg tatcggattg
     2521 aatccggtac gaggtattct gccggaaaaa acgatccttg aattagtgga acagctgaag
     2581 aaagagggtg cccgagtaaa ctacaagaaa aacccagatg gaacgaccag tccatatgag
     2641 ataaacgcta cgtatctaga tgcgttaagt tctccaacag atacgacgga atggagaagc
     2701 aaacgctttt tagctgctca ttcatttttg gtaacgttac caggcgtgcc ggcaatttat
     2761 attcaaagtg tgctgggaag tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat
     2821 cgaaaaatca atcggcaaaa atttttgtta gcagatattg agaaagagtt gcagacagat
     2881 ccattaagaa cagcagtata tggtgaattg acccagatgc tatctattcg aaaaaaagaa
     2941 aaagccttcc atccgaatag tttgatttac ttactggact cagcagattc ttttatcagt
     3001 tttattcgcg aagcgaagga agagaaagtc ttagttatcc ataatttttc caatcaatca
     3061 gcaggttatc ttttaccaaa aggagattac ctagacctgt tttctggaca aattatccat
     3121 tgcagtcaag aacgaaagtt gatattccag ccgtatgaat atatgtggct gaaaataata
     3181 aaataagagg tggaaaaaaa tgaaaaaaat tattgttact acctgtctgc tagctactgg
     3241 atttcttctt actgcttgtg gtgaaagtca aagctctaac gaatcacaac agacaacgat
     3301 tcaattcatg cactcttctg tcgaacagga acgattaagt gtgatcaatg aactggttgc
     3361 tgattttgaa aaagaaaatc cagacatcaa aatcaagcaa gtgccggtag aagaatcagc
     3421 gttcaataca aaaatcgtta cgttagctcg ttccggtaaa ttaccagaag taatggaagt
     3481 cagtcaggat tttgctaaag tgatggataa agatgaactg attgatcaga cggcagtcca
     3541 aaacgttatc gaggaagctg gagaagataa ttattatgac ggagccaaaa accttgttcg
     3601 ttcagaagat ggaaaaagct atattgctgc accaattagc ggctgggttc aaggtatttg
     3661 gtatgacaaa gaaaaattat cagaagctgg tttttcagaa ccagaaaatt gggatgatat
     3721 tttaaaaatt gctcaacatt tcacagataa agagaataaa caatatggaa ttgctatgcc
     3781 tactgctgaa ggaacaatga gtgagcaagc gttctctcaa tttgctttgt caaatggagc
     3841 aaatgttttg gatgatgaag gcgaggtaac gatcgatacg gaaaagatga gagaagcatt
     3901 gtcgttttat caaaacttat cccaatacac gatgccaggc tctaatgatg tgacagaaat
     3961 caaagatgct ttcatgaatg gaacagcacc aatggcaatc tattcaactt acattttgcc
     4021 ttctgtttat gaagcgggga actcagaaaa tatcggtttt gctatcccaa cacaaaaaga
     4081 tcaagctgtc tatggcacag tatctggatt gacgatttct tctggtctag acgagaaaca
     4141 aaaagaggct gctgaagcat tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg
     4201 ggtcttgatg tctcctggtg gtgctcaacc agtgaataaa caagtagtcg aactggatgg
     4261 ttacaaagaa aatgaagtca tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc
     4321 ttttgatgaa gtacaagtct ttggactagt cggggaaaag aatttcacga aaatgggaga
     4381 tatcacaagc tcaggtgtga tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga
     4441 tgtcgatcaa gctttagctg acgcacaaaa aaatacgaaa taacaagaag ttctgccaat
     4501 ggtgttcgaa aaaagtagat agctattggc agattatcag ggggaggttt caatgaatcg
     4561 tccaaggaaa cattctgact taaaattagc catgggactt ttagcaccaa gcatgctttt
     4621 attagctgtt ttagtgctga ttccaatgct ttccaatatt cagatcagtt ttttcgatat
     4681 gccgctgaat cctaactcag acgccgtttt tatcggatta agcaattatc ttgaaatttt
     4741 aagtgatagt gaattttatc gttctttagg tttaacagtt gcttacacat tcttagttgt
     4801 agcaggcagt acagctttag gattagccgt agctattttg cttaaccgca agttcagatt
     4861 taggaaaacg gtgcgttcac tgattttatt aagttatgtt acgccatcta tttctttgat
     4921 ttttgcttgg aaatatatgt tcaataatag ttatggaatc gtcaatgatc tgttagtaga
     4981 taggttgcag cttttcaacg aagcaccgct gtggttcgac cagccagttg ctagcttctt
     5041 tatggtcgtt ttgtttgcta tctggagata ctatccgtat gcgtttattt catttttggc
     5101 tattttgcaa acagtcgaca aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc
     5161 ttggcagaag ttcaagatcg tgacactgcc ggcaatcaaa ccagtactag caactgtgat
     5221 tgttttacga tctatctgga tgttctatat gtttacagat gtctatctac tgacgaataa
     5281 agtcaatatc ttaggcgttt atttgtatca gacagccttt gcgttcaatg atcttggaaa
     5341 agcagcagct atatcgattc tattgttcat cattattgca gcggtcgtct tgctctcacg
     5401 aaagaaggtg gacttgaaat gaaaaataaa aagagcagga aaatcttatt ttacgcgacg
     5461 attttgttgc tattagctgt caatttgttt ccatttttga tcatgctgat gacatcattc
     5521 aaggattcac aagaagcaat tgcaacgaca caaactattt ttccgaaaga atggactttt
     5581 gaacattatc aggatatttt caatgctgag atttttccat tcatcatgta tttcaaaaat
     5641 agtttagtga tctccttgat agcctccgta actgcagtag tgattgggat cagcggggca
     5701 tacgctttgt caaaactacg cttcaaagct cgctcgaaaa tcaatgctag cttttacacc
     5761 gtttatatgt tttccggtat tttgatcgtt gttccattat ttaaaatcat ttccggtcta
     5821 ggtttgtatg ataccaatac agcactgatt atcacgatgg tcgttcaaac acttcctact
     5881 gcgatattca tgctaaaaag ttattttgac acgattccga ctgatattga agaagcagca
     5941 atgatcgatg gtcttagccg gatccagatc attattcgga tcattatacc attggctatt
     6001 tctggaatca tttctgtctt cgtttattgc tttatggttt cgtggaatga ttatttattt
     6061 gcttcgattt tcctgtcgac ctccgaaaaa tttactttac caatcggttt gaacacatta
     6121 ttcagtacac cagactacat ttggggacga atgatggccg cttcattggt gaccgcttta
     6181 ccagtcatca ttatgtatgc gttatctgaa cgatttatta aaggtgattt agtcgaaggc
     6241 ggcgtaaaag gataaaaaga aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt
     6301 agaaccaaga atagccggat tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt
     6361 acgtatccga gtaattttcg ctactccaaa accaggaaca gaaattgtcg actttagagg
     6421 attaagtcca tttatcgatg aagaattttc agaagaatgg cgcctattct tgccaagaga
     6481 agaaggaagc caacgcggtg tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg
     6541 tacgatcgaa gaaacgggca gtttggtgac agagtatcaa aaaggagagt tagtctgcac
     6601 ttatggaccc attcgtgaaa cgctgattac aaacggtgtt gataattata aactacgtaa
     6661 acttgatcgg ttggatcaat ggaaaaatgc agtatgttac gatcctgctc aattcgcttt
     6721 aggtggtatc cgggaagcac atgttcgagc aggtgatgct gtagcaatct tcggtttagg
     6781 cgctatcggg caaattgcga ttcaattagc taaaaaagca ggtgctacta ccatcgttgg
     6841 gatcgatccc atagcgcagc gtagagaatt agcagaaaaa cacggtgccg attacacgct
     6901 agatcctatg aaagcagatg tcggttttcg tctaaaggaa ttgacagata aacgtggagt
     6961 agattcagtt attgaaacga gtggcagtgc tgccgcatta caagcttctt taatagggat
     7021 cggctatggc gggatcattt cttatgttgc ctttgctaaa ccttttccag ctgggtttaa
     7081 tcttggtcgc gaagcacatt tcaataatgc aaaaatcgtc tttgctcgag catctagtga
     7141 accaaaccca gattacccaa gatggaaccg taaacgaatc gaggacacat gttgggaatt
     7201 attgatgaac ggttacttgg attgtgaaga aatcattgat ccagttgtgc catttgcaga
     7261 aagcgcagaa gcttacatgc gttatgtaga ccagcaccca gaagaaagta tcaaaatggg
     7321 cgttcaagtc cgaggaggag aacaatgaaa ttcggtacgc aagatcaagc atttttccca
     7381 agagacattt tagaaaaatt tagatttatc aaagactgtg gatttgaaga atacgaaatc
     7441 gacggtaagt tgttaataga aaatatcgac acagtcaaac aggcaatcaa agaaacaggg
     7501 atcccagtag tgacagcttg taacggctac gatgggtgga tcggtgactt tatcgaagaa
     7561 cggcgtctta atggtgtacg tcaaattgct gagattttgc gtgcactaag cgaggttgga
     7621 ggcaaaggaa tcgttatccc agcagcttgg ggaatgttca cttatcgatt accgccgatg
     7681 gtttcacccc gaagcaaaga aggagactat aaagcagtca gcgaatcact tcgttattta
     7741 gataaagtag ccgaagaaac aggtacggtt atttatttgg agcctttgaa ccgataccaa
     7801 gaccatatga tcaatttgtt agcggatgcg cgtcattaca ttgactctaa tcaattgaaa
     7861 catgtgcaga tcatcgctga tttttatcat atgaatattg aagaagatga tatttcaaaa
     7921 gcattgacta cgcaaaaaga gtatgtgggt catatccatt tagcagataa tcatcgctac
     7981 caaccaggat ctggcgcaat tgattttact cgccattttc aaacgctact tcaaaatgag
     8041 tatgaaggtt atatggtata tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat
     8101 caggaatcat tgcaatactt gaaaaactgt ctggaaacag cgcgtcattt aaatgattta
     8161 gagaaagaat agagaaagaa taacaataag taaataagca aaggagggag aaataaatga
     8221 agaaagtagc aatcataggc gctggacaag tagcagaaaa agtacatgct gcttattatc
     8281 aaaaaagatc agatgtctta gaattggccg ctgtggttga tccagtcagc cagcgagcag
     8341 aagacttttg ccgacgtaca ggattttctc atgcgtatac atcagttaca caaatgctga
     8401 atgagatttc tcccgatatc gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg
     8461 tattggctgc cctcgaagct aatgcttcag tgatgtgcga gaaaccacca gctatgacag
     8521 ctgatcaagc aagaaagatg gcagagacag ccgataaaaa cgggcagatt ttggcttatg
     8581 gattccagca tcgtttttct gacgaagcaa caatgatcaa gaatcatctt gatgttctcg
     8641 gggatattta ttacgtagaa gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa
     8701 cgtttatcga caaagaactt caaggaggcg ggcctttgat cgattatggc attcacatgc
     8761 ttgatacagc gatgtatatt actggatttc ctaaagtaaa aagcgttcgg gcgtacagct
     8821 tccaaaaaat tggtacgaaa aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat
     8881 tcacagtaga agatgcaatt tttggcacac tggaattaga aaacggcaag atcatccaac
     8941 tgaatacttc gtttgctttg aatatcagag aagacaaact gaatgtggct ttttgtggcg
     9001 atcaggcagg tgctaatctg tatccaggcg aactattcga agatgtagat ggaaagctgg
     9061 tttccttaca aaagaccacg ccagttaatg acccacatga actaagtatg gctcattttg
     9121 tgaatcgggc gctcggtgac cagactcaga tgattgcaga tggctggcaa ggatatgtca
     9181 tccagaatct ggtggaaaat ttatatcgtt cggcagaaac aggagaagtt attaccttat
     9241 gaaagtagaa gaaaaacagt ttgatttaga gtatatcaat aagtatgcga gtcagatgac
     9301 cattggaaat ggattttttg gtatcagagg aagccaggag gaagactatc ggaatcaagt
     9361 aaggggaatg tttgcagctg gtgtatataa ccgaccttta ggagcagaat cagccgagtt
     9421 agtgaatctc ccagatgtca tcagatggga aataaaaatc gacggtgaac atttttctct
     9481 tgagacgatg aaagttcttg catacgagcg ggcactagat ttagaaatgg gagagcttaa
     9541 acgctatgcg ctcattgaaa ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt
     9601 tgctgatcaa aataatctcc atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga
     9661 agactgccaa gtagatgtga aaacgggtat cgatggacag caaaccaatt ttggtacaca
     9721 gcagttggtt gaaaaagaac tgcgagtatt tggtgacgat ttgatcagtg catcctacca
     9781 gacaacagaa agtaaaattg atattgggat tgcagctaaa ttcaataaag caggtgtatt
     9841 ccaagcaaaa aatcgccgga tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc
     9901 ctttacgctg gagaagatca tcgtgctctc tacctctctt cagcaaacag acccgtttaa
     9961 gaaaagtatt gcccaagccc agaaaagcgt tagttataca gaaattttag agcagtcgat
    10021 gtcttattgg acagattttt ggcatcagca tcggataacg atccaaggcg attcttcttt
    10081 cgatcagtta gcgatggact tcgctgctta ccatttacag attatgactc cccgcaatga
    10141 tccaagtgtt agtgtagggg cgaagggact aactggcgaa ggttataaag ggcatgtttt
    10201 ctgggatacg gaaattttta tcctgccttt ctttttatat cagtttcctg aagtagcaaa
    10261 gaatttgctg ctgtaccgtt atgatcgatt agaaggcgca cggcaaaaag caagagatta
    10321 tcactatcaa ggtgcacttt tcccatggga aagcgcacac actggtcagg aagagacacc
    10381 caaatttgct gcattgaata tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc
    10441 agaacatcat atcagtgcag accttgctta tgccgttcaa gagtatgtcc aagcaacaga
    10501 ggatcaatcg tttttagaaa caaagggaaa aacgctcatt catgaaacag cagctttttg
    10561 gctgagcaga gctgtaaaag tcgatgatca tttagagata cacagagtca ttggtccaga
    10621 tgaatacaca gaatatatcg acaataatgc atatacgaac tatctggcgc attataatgt
    10681 ggccttagct gttaaatatc aagctggcga tccgcttttt ttgaaagaat gtcaaaattt
    10741 tttagataag ttatatttgc cagtgcctaa caaagaacag ctgattccgc aagatgacac
    10801 gtatctcagt aagccagtta ttgatttgac cacttacaaa gaacgtcctg gcagccagtc
    10861 tattttactt gattatgccc gtagcgaagt aaatgaactc caagtcagca agcaagcaga
    10921 tcttgtcatg ctgttatact tattacctga gtatttttct caaccaatcg tttctaagaa
    10981 tttgcattac tatgaaaaca aaacaatcca cgactcatcg ttgagtaaag cgattcacgc
    11041 catagaggct tgccgtatcc aagaaatgga atgggcgtat catttattcc aagaagcgtg
    11101 cctgattgat ttaggaccag atccgcattc atcagatgac ggaattcatg ctgcttcatt
    11161 aggagcacta tggctagcag ttatatttgg tttttcaggt attacagtaa cgaatggaac
    11221 tctttctatt tctccaagac ttccttcagc atgggaaaaa atttcttttc cttttatttg
    11281 gaaaaatcac aggttgctat ttgttttgac aaaaaaacaa atagtaataa aaaaagagac
    11341 gaaagaaaca ttacctattt ttgtcaatgg agaccaatac cagctggaaa aagaactgat
    11401 cttggaacgg aggggaaaat aatgcgtatt ctgattgcgc cagattcttt caaagaaagt
    11461 ctgacagcct tagaggcagc acaagccatt caatcaggtt ttcaagaagt ttttgttcag
    11521 gctgaatatg atctgctacc tataggcgat ggaggagaag gaacggtttc atcattagca
    11581 gacagtttaa ggttgaagcg gcaaacagca gtcgttacta atgcatttgg caaaaacaaa
    11641 gaaatccagt acgcttcaga cggtcaaact gcgatttttg aaatggcaga aatcgtcgga
    11701 ttgaaagatg tcccactaaa ccaacaacag ccacttaggt tatccacgcg aggagtagga
    11761 gaactgatac gacaattgtt gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga
    11821 agtagtacca acgatggcgg catcggcatg gcagaaggtc taggttatgc tttttacgat
    11881 caagataatc aaaaagtaga agcactggga gaaaatctag gtaaaattgc tacagttgat
    11941 aaaacgaacg tgcctattga gctgattaaa gccaaaatca ctgtcattac tgatgttact
    12001 aatcttttat gcggaaaaga aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa
    12061 gaaaatgaat tagcagtggc tgaccaagcc atgcatgatt tttatatgag atttgcccct
    12121 caagtaatcg aggcatcggg cagtagtgct ggtggtggaa tgggtgcagg actgttagca
    12181 tttgcttcag ggaaaatagt ctctggcatc gactacgtat tggatttact ggagttcgat
    12241 acgggtacag caagcagacc tcgtaatcgt aggcgaagga cgtctagatg gacaaagtct
    12301 aaaaggaaaa gcgccagttg ggattgctag aagagtacca gagggcgttc ctgtgatagc
    12361 tatttgcgga agtgttggta aaggaagcga aaaagcagca gatgttggca tttcggccgt
    12421 ttttccgatc cttcctgcag ctgtgccttt acaagaagct ttacagcaag ccactgataa
    12481 tcttcatcgc acagctaaaa acatcgcatc tttacttcaa gtcgttcatt ttcaaggagg
    12541 taatcgatga aaatcaaagc agcactattc gatttagatg gcgttcttgt agacacagct
    12601 cgttatcatt acgaagcgtg gctggtttta gctaaccagc tgtccattcc attcactgag
    12661 aaagaaaacg agcaactgaa aggaatatcc agaaccgagt cactagagcg attgctgtca
    12721 tttggaaaaa tggaacagaa attctcagaa aaagagaaat cagcctttgc tgagcaaaaa
    12781 aacaatctat atcttcaagc gattcaaaaa atggatgaaa catccgtttt gcctggtgca
    12841 atagctgttc ttgagtactt gaaaaaaaca aacatcaaga tcggattggg atcagcaagc
    12901 aaaaatgcac gcctcatttt agaaaaaacg aatttgacaa gttattttga tgtccttatt
    12961 gacggcacgc aagtatcaaa agcaaaacct gatccagaag tttttctcaa aggagcacag
    13021 caattgaatg taccgccaaa tgcatgcttg gtcattgaag attcagaagc tggttgtcaa
    13081 gcagctttgg ctggtaacat gcatgtattg ggaataggtg aaaacataaa tcttccttct
    13141 gctgaatatg tgattcctga tttaaccgtt tttgatcaag tacgttcgtt ttggcattta
    13201 tcagaagccg tatcacgtat ataatagttt ccgttttttc tgtcacaagc aaaacgttat
    13261 cctccttttg gaaatcaagg tatgatgaga aaaaaggggg attttatatg acaaaaaaag
    13321 cagttttcgc aggcggatgc ttttggtgta tggtcaaacc atttgaagag cagccaggga
    13381 tcatttctgt gacatccggt tatactggcg ggcatgtacc aaatccaact tatgaacaag
    13441 taacaacagg cacaacaggg catacagaag cagtagagat cgaatatgat ccagaagtta
    13501 tttcttatga tcagttggtg gaaatctatt ggcagcaaac cgatccgact gacgcattag
    13561 gacagttcgc tgatcgcggt gattcttatc gtccagtgat ctactataat gatgaagaag
    13621 aacgaaatat tgcagagcgt tcaaaacaag cattgcaaga cagcgggcga ttcgataagc
    13681 caatcgtgac aaaaatcgaa ccaagaagcg aattttatcc tgcagaagat taccatcagg
    13741 attattacaa gaaaaacaag ctccattaca atctttatcg agaaggttct ggacgtgcag
    13801 gtttccttcg tcgacattgg ggcgtcaaga aataagaagc cgaaaaagct acttatggaa
    13861 caccgtatat cagaggttgt aaaaccagca ttttgagcta atactgaggg tcagctgatt
    13921 gaacaccatt tagtggagag gttgtgaacc tgtcgcctag ctccaagtca caaggatttg
    13981 gtaagagaac actatatatc agaggttgtg acaagtcgtt tgtcacaacc ttttttcaat
    14041 acctaagatg aaggacaatc agcaacgcac tgctcaaaaa aggaatgaaa ggcagctttt
    14101 ccaacttttt tttatgacag ataaagtaaa cagaaaaaac ggatattcca aacagactag
    14161 caagaaacaa gatctgaagc aactcttcta gagacaagcc accactccaa gaaagcatca
    14221 acagagtatc gccagctccg agataggatt gaccatagcg aaggcacaca ccgcctacga
    14281 taaacgttaa cagcacactt tgccattgga agacgccggc ctgcaaccaa aaaatccata
    14341 aaattgccca agaacagaat agcgtgtgag aatggaccat gaagtaaaat aaatccatca
    14401 acgaaaatag caaagccgaa agcatccata tgaacgtcga aaaatttctt gcggaagaaa
    14461 aagtaaagaa ccaggcaaaa agaccaccac ataaggcttc tgccaacaaa tagtagaagg
    14521 gcaaccgaca cctgcaatag cggcagcgaa atcgttggac gatgatcgag agaatcggaa
    14581 tcaattcata ccaagaaagc gaacgacagc atttgacaca atgagatcta ggataaataa
    14641 tagagtgacc taacggaatc cgctgggcgg ttaaacataa aaatgatccg atacagcaac
    14701 caatgataaa ataaagtaac acgccatctc ccccttttca aaaccaagat acgcaagctt
    14761 aaactaaatt tttgtacata cataaaattt gaggtgaaaa aatgaacgtt tcaatcggat
    14821 tagatatcgg aaccacacaa accaaagcag ttgctttcac agatgaagcc caagaagcag
    14881 catccgctta ttttcgctac cctttgatcc aagaaacaca agggatggca gaacaagatc
    14941 aggaattgat ctttcaaggt gtctgcaccg ttataaaaga agtaattaaa cagttgcctt
    15001 cagacacaat cattcaattt gtctccttct cctctgcgat gcatggcctg atattgttgg
    15061 atgaagagaa aacaccgtta tctcgaatga tcacttgggc agataaccga gcagcggacg
    15121 aagcagaaaa actgaaatat accgtgcttg gcaaagaatt ttatcggcag actgggacgc
    15181 cgattcatcc aatgacacca ttgaacaaaa tcaagtggct gcaaaacagc caacctgaat
    15241 tattcaaaaa aactgcttat tttgtcggta tcaaagacta tatcttttat agattgtttg
    15301 gcgaactgat cagcgactac ggaacagctt caggaactgg atattttgat atccatgaat
    15361 tccaatgggc agaagaagtg cttgactact tgaacattcg aatagagcaa ttgccgcaag
    15421 tctttccttc cacctatcaa gtaacaggtc taccatcaaa aacagcagca gaactaggtt
    15481 tgccaaatga tatatcattt gttttgggag gggcagacgg gccgctttct aatctagggt
    15541 taggtgcttt tggtgatacg atcgccgctt tgaccgtagg aacgagcgga gcacttcgtt
    15601 ttattactac tcagcctcag ttccatccag aaatggagac gttttgttat gttctggatc
    15661 agaaacattg ggtagtggga ggagcgacta gcaatggtgc tggcatcttt gattgggcag
    15721 ggcatacatt tttacaggaa gtcgttcaat ctgctaaaga aaaaggagaa aatccatatg
    15781 atgcactgct ttcggctatc gcttttgtgc cagcgggtgc caatggtctg atttttcatc
    15841 cctatttact tggtgagcga gcaccgcttt gggacgcaga tgcgtttggc agttttatcg
    15901 gtttacagcg gcaacataca gaaaaagaaa tgatgaaggc gattctagaa gggatctgtc
    15961 tcaaccttta tcggatttta aaaggagttt gccaggaaaa tcagctcaca gagatccgtg
    16021 ctacaggcgg gtttgctcaa tctccgattt ttcgtcagat gatggctgat gtactgggtt
    16081 acccgcttgt tttccctact gtcacagaag catccgcatt aggagctgtg attttaggct
    16141 ggagaagcat cggaaagatc caaagtatca gtgaagcaca ggaattgata gaattgaaag
    16201 agcaagttgg agtacaagaa actgatcata aaaaatatca gaaaatctat ccattatttg
    16261 tttcgacaca aaagcattta gcacagactt atcagcaatt tgctcaactg cgagaagaat
    16321 tagctgattc tgaaaaatga ttggctttac ggataaaaaa gcgaactgaa aatcgttctc
    16381 aggtaaatga ttttgttgta cttttcattg aaagtctact attattttaa gtgtaagaaa
    16441 atgttaggag gcaagaaaga tgaaattcca acaaaccaaa gaagtattaa atcaattagt
    16501 tgcagatcta agccaaatgt ctgttgttgt tcaccaaaca cattggtaca tgagaggacc
    16561 aggtttcctt acattgcacc caatgatgga tgagttcatg gatgatttga acgctcagtt
    16621 agatgaagta tccgaacgct tgatcacatt agacggttca ccttattcta cattgcgtga
    16681 atttgcagat aacacaaaaa tccctgatga aattggccgc tgggatcgta ctattccaga
    16741 acgtttggaa acacttgttg caggctatcg ttatctagca gatctttacc aaaaaggaat
    16801 cgaagtttct ggagaagaag gcgacgatcc aacacaagat atctttatcg aattcaaaac
    16861 agccacagaa aaacgtatct ggatgatcca agctcatttg ggcaaagcgc cagagatcga
    16921 cgcgtaaaaa ctgttgttga acttgaccga aaaagaggtt gtgaaatcag cgttttgacc
    16981 tgagtattag agaaacaaac gtcaaaatag ttcctcatat tttgccgttt tttgagctaa
    17041 taccgagggt cagctgattg aacaccgttt accaagaggt tgtaagcctg ccgttcagct
    17101 ccgagtcaca agaaacaatt ttaaaaaaca gcttcttatg ttttatggat attgtgactt
    17161 gtgaccgagg agttggcagg agaacaccgt ttattcaaaa taaggggttg tgacaagact
    17221 tttgtcacag cctctttttg tttttcattt tgacaggaaa cgaccctttt atataaaaga
    17281 aagaaaagaa gtaaagtttc atataaattt ttcaatcaag cagaaaaacg ttgacattga
    17341 ctagattagc gggtaatatg ttaaatggta ttgtttgccc cacaaagagc cccggaaact
    17401 cgaagtgtat gtcaaacatc agaacttaaa ttggaggaaa ataacgtgcg tacaacatat
    17461 atggccaaac caggcgaagt agaacgtaaa tggtatgtag tagacgcaac tgatgttcca
    17521 ttgggacgtc tttcaacagt tgttgcatct gtactacgtg gtaaaaataa accaactttc
    17581 acacctcatt tggatactgg cgatttcgtc atcgtcatca atgcagacaa agtgaaatta
    17641 acaggtaaaa aagcgactga caaaatttat taccgtcaca gcaactaccc aggaggattg
    17701 aaatcaatca ctgctggcga attgcgtgct aaaaattctc gtcgtttgat cgaaacttct
    17761 gtaaaaggta tgcttcctaa aaacacttta ggccgcaaac aatttacaaa attgaacgta
    17821 tacggtggag cagaacatcc acatgcagca caacaaccag aagttttaga catcacaaac
    17881 ttaatctaat aggagggaat ttcattggca caagttcaat atatcggcac aggccgtcgt
    17941 aaaaacgcag ttgctcgcgt acgtctagta ccaggaactg gcaaaattac tgtaaataaa
    18001 aaagatgttg aagaatacat tccacatgct gacttgcgtg aagtaatcaa tcaacctttc
    18061 gctgttactg aaacaaaagg cgcatatgac gtatttgtta acgtaaacgg cggaggatac
    18121 gctggacaat caggagctat ccgtcatggt atcgctcgtg cattgctaga agttgatcct
    18181 gacttccgtt tagctcttaa acgtgctggc ttacttactc gtgacgcacg tatggtcgaa
    18241 cgtaaaaaac caggtcttaa gaaagcacgt aaagcttcac aattctcaaa acgttaatat
    18301 cctttatata tcaacgtttc tggcactttt caattatttg gaaagtgctt cttttgtttt
    18361 taagaacaca tttaagaaca catatcaatc acacttattc aatcctctct gaaaagcttc
    18421 aaaaacagcc ttatcaacta tatctggtgt gttcacatat atctttgttg tagaaatatc
    18481 tgagtgtgtt aaagcatttg aaatttgatt catatctgct ccaccttgtc tagctatagt
    18541 tgcataagta tgacggagtt tgtgtggaga taaatgaact aagtcaggat gccttctttt
    18601 aattgaattg attcgatagt ttaggtaatc ggcatgaaca ggacaattta cttctccaga
    18661 tggtttagta taagtaaaga gatactgatc tgacgtttgc ttaattttta atttcaacaa
    18721 ttgatcagct tgtaccgatt tccattcgct tagcaatgtc attacaactt ctggtacttg
    18781 aatgacggta tcttttcgac cttttgtaaa cttttttctt tgatatttat ctaaagtatg
    18841 tttaagacgg accgtttgtt tttcaaaatc aatatatttc cattgcaaag cgtaagtctc
    18901 actttttcta tcaccaagat acagggtaat cataaaaagt gtgtaatcat gaaaagtaag
    18961 taaatgatta ttaaaatcat catttactgc ttctatccaa ttagtaagtt cttttgatga
    19021 tagtgcttgc ttaccttctt gtattctttt ttcttctaaa gcattttttt taggtgcagt
    19081 gatactttga ataattttag tagttcgatt aaattcgata taatttagta tttctgcaat
    19141 atcgaacatg gaacgaacat atccttttat gatttttata tttgcatatt ctttcgattt
    19201 tttggttaat tcatctgata ttatctcagt attgatattt agataattca ttgcgtattc
    19261 accaaacata ggtaaaatat gcaaccgaaa tatatctttt gtattactaa tggttatatc
    19321 actaggaggc ctgtttgatc taattgtttg acctaa
//
LOCUS       DAXWLB010000045        18481 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXWLB010000045 DAXWLB010000000
VERSION     DAXWLB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.045"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(27..851)
                     /locus_tag="ACGE4H_001202"
     CDS             complement(27..851)
                     /locus_tag="ACGE4H_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFF8364631.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(824..1504)
                     /locus_tag="ACGE4H_001203"
     CDS             complement(824..1504)
                     /locus_tag="ACGE4H_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8364632.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1527..2465)
                     /locus_tag="ACGE4H_001204"
     CDS             complement(1527..2465)
                     /locus_tag="ACGE4H_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8364633.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2710..3561)
                     /gene="ispE"
                     /locus_tag="ACGE4H_001205"
     CDS             complement(2710..3561)
                     /gene="ispE"
                     /locus_tag="ACGE4H_001205"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFF8364634.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3715..6288)
                     /gene="mprF"
                     /locus_tag="ACGE4H_001206"
     CDS             complement(3715..6288)
                     /gene="mprF"
                     /locus_tag="ACGE4H_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8364635.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6429..7235
                     /gene="proC"
                     /locus_tag="ACGE4H_001207"
     CDS             6429..7235
                     /gene="proC"
                     /locus_tag="ACGE4H_001207"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFF8364636.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7314..8870)
                     /gene="rny"
                     /locus_tag="ACGE4H_001208"
     CDS             complement(7314..8870)
                     /gene="rny"
                     /locus_tag="ACGE4H_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFF8364637.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9281..10330)
                     /gene="recA"
                     /locus_tag="ACGE4H_001209"
     CDS             complement(9281..10330)
                     /gene="recA"
                     /locus_tag="ACGE4H_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFF8364638.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10439..11680)
                     /locus_tag="ACGE4H_001210"
     CDS             complement(10439..11680)
                     /locus_tag="ACGE4H_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFF8364639.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11920..12498)
                     /gene="pgsA"
                     /locus_tag="ACGE4H_001211"
     CDS             complement(11920..12498)
                     /gene="pgsA"
                     /locus_tag="ACGE4H_001211"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFF8364640.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12707..13630)
                     /locus_tag="ACGE4H_001212"
     CDS             complement(12707..13630)
                     /locus_tag="ACGE4H_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFF8364641.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14369..15655)
                     /locus_tag="ACGE4H_001213"
     CDS             complement(14369..15655)
                     /locus_tag="ACGE4H_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8364642.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15648..16916)
                     /locus_tag="ACGE4H_001214"
     CDS             complement(15648..16916)
                     /locus_tag="ACGE4H_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFF8364643.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17168..17629
                     /gene="mscL"
                     /locus_tag="ACGE4H_001215"
     CDS             17168..17629
                     /gene="mscL"
                     /locus_tag="ACGE4H_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFF8364644.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17688..18134)
                     /locus_tag="ACGE4H_001216"
     CDS             complement(17688..18134)
                     /locus_tag="ACGE4H_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364645.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18227..18472)
                     /locus_tag="ACGE4H_001217"
     CDS             complement(18227..18472)
                     /locus_tag="ACGE4H_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFF8364646.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5270 a         4039 c         3234 g         5938 t
ORIGIN      
        1 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
       61 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
      121 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
      181 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
      241 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
      301 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
      361 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
      421 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
      481 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
      541 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
      601 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
      661 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
      721 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
      781 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
      841 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
      901 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
      961 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
     1021 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
     1081 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
     1141 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
     1201 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
     1261 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
     1321 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
     1381 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
     1441 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
     1501 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
     1561 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
     1621 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
     1681 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
     1741 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
     1801 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
     1861 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
     1921 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
     1981 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
     2041 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
     2101 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
     2161 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
     2221 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
     2281 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
     2341 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
     2401 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
     2461 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
     2521 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
     2581 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
     2641 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
     2701 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
     2761 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
     2821 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
     2881 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
     2941 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
     3001 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
     3061 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
     3121 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
     3181 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
     3241 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
     3301 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
     3361 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
     3421 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
     3481 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
     3541 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
     3601 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
     3661 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
     3721 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
     3781 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
     3841 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
     3901 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
     3961 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
     4021 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
     4081 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
     4141 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
     4201 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
     4261 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
     4321 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
     4381 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
     4441 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
     4501 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
     4561 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
     4621 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
     4681 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
     4741 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
     4801 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
     4861 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
     4921 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
     4981 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
     5041 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
     5101 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
     5161 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
     5221 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
     5281 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
     5341 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
     5401 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
     5461 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
     5521 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
     5581 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
     5641 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
     5701 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
     5761 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
     5821 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
     5881 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
     5941 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
     6001 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
     6061 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
     6121 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
     6181 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
     6241 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
     6301 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
     6361 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
     6421 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
     6481 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
     6541 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
     6601 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
     6661 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
     6721 cattaacaga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
     6781 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
     6841 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
     6901 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
     6961 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
     7021 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
     7081 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
     7141 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
     7201 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
     7261 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
     7321 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
     7381 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
     7441 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
     7501 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
     7561 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
     7621 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
     7681 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
     7741 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
     7801 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
     7861 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
     7921 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
     7981 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
     8041 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
     8101 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
     8161 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
     8221 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
     8281 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
     8341 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
     8401 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
     8461 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
     8521 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
     8581 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
     8641 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
     8701 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
     8761 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
     8821 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
     8881 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
     8941 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
     9001 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
     9061 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
     9121 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
     9181 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
     9241 acctactctt cagactagcc gtttttgttt atcataattt ttattcttct actaaaggca
     9301 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
     9361 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
     9421 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
     9481 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
     9541 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
     9601 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
     9661 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
     9721 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
     9781 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
     9841 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
     9901 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
     9961 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    10021 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    10081 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    10141 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    10201 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    10261 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    10321 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    10381 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    10441 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    10501 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    10561 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    10621 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    10681 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    10741 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    10801 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    10861 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    10921 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    10981 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    11041 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    11101 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    11161 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    11221 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    11281 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    11341 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    11401 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    11461 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    11521 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    11581 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    11641 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    11701 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    11761 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    11821 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    11881 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    11941 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    12001 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    12061 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    12121 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    12181 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    12241 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    12301 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    12361 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    12421 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    12481 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    12541 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    12601 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    12661 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    12721 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    12781 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    12841 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    12901 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    12961 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    13021 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    13081 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    13141 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    13201 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    13261 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    13321 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    13381 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    13441 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    13501 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    13561 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    13621 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    13681 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    13741 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    13801 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    13861 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    13921 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    13981 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    14041 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    14101 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    14161 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    14221 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    14281 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    14341 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    14401 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    14461 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    14521 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    14581 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    14641 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    14701 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    14761 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    14821 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    14881 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    14941 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    15001 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    15061 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    15121 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    15181 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    15241 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    15301 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    15361 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    15421 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    15481 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    15541 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    15601 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    15661 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    15721 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    15781 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    15841 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    15901 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    15961 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    16021 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    16081 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    16141 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    16201 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    16261 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    16321 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    16381 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    16441 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    16501 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    16561 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    16621 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    16681 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    16741 tttgtctgat ccggataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    16801 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    16861 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    16921 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    16981 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    17041 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    17101 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    17161 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    17221 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    17281 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    17341 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    17401 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    17461 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    17521 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    17581 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    17641 aaaaaaagaa agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact
    17701 tgttcctttt tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc
    17761 ttgaatccaa acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt
    17821 ttttctggaa aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa
    17881 ccatctttac ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct
    17941 tcatatactc gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa
    18001 gcttgtccat caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact
    18061 gcgattcgtt ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt
    18121 acctgatatt ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca
    18181 cacaaaaaag caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag
    18241 ttaaaaattc gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt
    18301 caaaagaatt ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta
    18361 aaacgccgcg gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc
    18421 tgccgatacg accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca
    18481 c
//
LOCUS       DAXWLB010000046        18125 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXWLB010000046 DAXWLB010000000
VERSION     DAXWLB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.046"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            167..361
                     /locus_tag="ACGE4H_001218"
     CDS             167..361
                     /locus_tag="ACGE4H_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364647.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="ACGE4H_001219"
     CDS             630..929
                     /locus_tag="ACGE4H_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364648.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="ACGE4H_001220"
     CDS             complement(1026..1460)
                     /locus_tag="ACGE4H_001220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364649.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="ACGE4H_001221"
     CDS             complement(1692..2402)
                     /locus_tag="ACGE4H_001221"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFF8364650.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="ACGE4H_001222"
     CDS             complement(2392..3252)
                     /locus_tag="ACGE4H_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFF8364651.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="ACGE4H_001223"
     CDS             complement(3257..3895)
                     /locus_tag="ACGE4H_001223"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFF8364652.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="ACGE4H_001224"
     CDS             complement(3910..4209)
                     /locus_tag="ACGE4H_001224"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8364653.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="ACGE4H_001225"
     CDS             complement(4252..5736)
                     /locus_tag="ACGE4H_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFF8364654.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="ACGE4H_001226"
     CDS             complement(5756..6217)
                     /locus_tag="ACGE4H_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8364655.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACGE4H_001227"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="ACGE4H_001227"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFF8364656.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="ACGE4H_001228"
     CDS             7520..8290
                     /locus_tag="ACGE4H_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8364657.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="ACGE4H_001229"
     CDS             complement(8563..9390)
                     /locus_tag="ACGE4H_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364658.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="ACGE4H_001230"
     CDS             9958..11193
                     /locus_tag="ACGE4H_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8364659.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="ACGE4H_001231"
     CDS             complement(12192..12452)
                     /locus_tag="ACGE4H_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFF8364660.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="ACGE4H_001232"
     CDS             complement(12479..13780)
                     /locus_tag="ACGE4H_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8364661.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="ACGE4H_001233"
     CDS             complement(14072..14530)
                     /locus_tag="ACGE4H_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8364662.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15874)
                     /locus_tag="ACGE4H_001234"
     CDS             complement(14543..15874)
                     /locus_tag="ACGE4H_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFF8364663.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17327)
                     /locus_tag="ACGE4H_001235"
     CDS             complement(15885..17327)
                     /locus_tag="ACGE4H_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8364664.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="ACGE4H_001236"
     CDS             complement(17314..18078)
                     /locus_tag="ACGE4H_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFF8364665.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         5777 a         3737 c         2796 g         5815 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attaggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtcgaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgtatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatc
//
LOCUS       DAXWLB010000047        17935 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXWLB010000047 DAXWLB010000000
VERSION     DAXWLB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.047"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(195..773)
                     /locus_tag="ACGE4H_001237"
     CDS             complement(195..773)
                     /locus_tag="ACGE4H_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8364666.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(793..1461)
                     /locus_tag="ACGE4H_001238"
     CDS             complement(793..1461)
                     /locus_tag="ACGE4H_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364667.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1476..2546)
                     /locus_tag="ACGE4H_001239"
     CDS             complement(1476..2546)
                     /locus_tag="ACGE4H_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364668.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2717..4081)
                     /locus_tag="ACGE4H_001240"
     CDS             complement(2717..4081)
                     /locus_tag="ACGE4H_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFF8364669.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(4078..5637)
                     /locus_tag="ACGE4H_001241"
     CDS             complement(4078..5637)
                     /locus_tag="ACGE4H_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364670.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5693..8173)
                     /locus_tag="ACGE4H_001242"
     CDS             complement(5693..8173)
                     /locus_tag="ACGE4H_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364671.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8157..8492)
                     /locus_tag="ACGE4H_001243"
     CDS             complement(8157..8492)
                     /locus_tag="ACGE4H_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364672.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8506..9540)
                     /locus_tag="ACGE4H_001244"
     CDS             complement(8506..9540)
                     /locus_tag="ACGE4H_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFF8364673.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9630..10310)
                     /locus_tag="ACGE4H_001245"
     CDS             complement(9630..10310)
                     /locus_tag="ACGE4H_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8364674.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10594..11697)
                     /locus_tag="ACGE4H_001246"
     CDS             complement(10594..11697)
                     /locus_tag="ACGE4H_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFF8364675.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12326..12796)
                     /locus_tag="ACGE4H_001247"
     CDS             complement(12326..12796)
                     /locus_tag="ACGE4H_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFF8364676.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13126..14766
                     /locus_tag="ACGE4H_001248"
     CDS             13126..14766
                     /locus_tag="ACGE4H_001248"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFF8364677.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14920..16242
                     /locus_tag="ACGE4H_001249"
     CDS             14920..16242
                     /locus_tag="ACGE4H_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFF8364678.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16310..16744)
                     /locus_tag="ACGE4H_001250"
     CDS             complement(16310..16744)
                     /locus_tag="ACGE4H_001250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364679.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16809..17645)
                     /locus_tag="ACGE4H_001251"
     CDS             complement(16809..17645)
                     /locus_tag="ACGE4H_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8364680.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         5244 a         3663 c         3148 g         5880 t
ORIGIN      
        1 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
       61 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
      121 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
      181 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
      241 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
      301 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
      361 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
      421 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
      481 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
      541 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
      601 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
      661 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
      721 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
      781 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
      841 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
      901 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
      961 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
     1021 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
     1081 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
     1141 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
     1201 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
     1261 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
     1321 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
     1381 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
     1441 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
     1501 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
     1561 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
     1621 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
     1681 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
     1741 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
     1801 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
     1861 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
     1921 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
     1981 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
     2041 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
     2101 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
     2161 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
     2221 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
     2281 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
     2341 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
     2401 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
     2461 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
     2521 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
     2581 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
     2641 ttcattatat gaatttttta ttctcgattg attaatattt attatatccc cacacaataa
     2701 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
     2761 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc
     2821 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
     2881 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
     2941 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg tattgtggaa
     3001 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
     3061 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
     3121 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
     3181 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
     3241 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
     3301 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
     3361 gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt acggatacta
     3421 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa ttgatttgct
     3481 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa
     3541 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
     3601 attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
     3661 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
     3721 gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca taggttatgg
     3781 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
     3841 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
     3901 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
     3961 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
     4021 ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc acgattttca
     4081 tgttttattc atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa
     4141 ataggggaaa tcataattat attcgttgct agtgaataca aaaactttag cggacttctg
     4201 tgacgggtac tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta
     4261 ttcgactaaa tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat
     4321 atccgccatt agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg
     4381 cttgatcagc gtccgcaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc
     4441 ctcgtctact tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa
     4501 aaccaggaat tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa
     4561 aatattcatc catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc
     4621 aatcttttgc gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg
     4681 tttaaacgtt cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa
     4741 ctcctggaca tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa
     4801 aaccaacgtt tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa
     4861 tgtatggagg tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata
     4921 atctggtttg gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc
     4981 aaatttcaat cgtccttctt ctgtaatagt aaaactaaac tctttttgca gcagatctaa
     5041 aaaagtgttt cctgataaat cactgtattc ttttttgttt tcaagataag gacgtccaaa
     5101 ccaaaataat tggaaataaa aataacaaat ttgactttct ggaccggtca gccgcccgct
     5161 ttttattttc agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa
     5221 agtagccgaa ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc
     5281 caacagataa agaagtatct gatattttgt agaatagcgc aagtagcaat gataataaac
     5341 atcgactgct gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat
     5401 ttccaattgt ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt
     5461 agcccgatca actgataagc gggtaagtac gttattaacg gaaatctctc ctttttcctc
     5521 taataaaagt tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag
     5581 ttccaagtcc actcctccaa gcatttctga ttttaacagc agaagtttcc ttctcatcct
     5641 tgaaaaagag gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg
     5701 ttggcccagc tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat
     5761 tcgtactgct atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct
     5821 ctcctttagc tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt
     5881 gttttgatcc agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat
     5941 tcttaaataa agaagtcaat gtccctgtca gttgccaagg agacgtgctt ggaagccgag
     6001 tatcccgcac atcaaaagaa aaatcgcctt ttgcttcata ataacttgtc ttcaacggtt
     6061 tggggagatc gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt
     6121 tggggacaag aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat
     6181 ctgctaatgt aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta
     6241 cagaagtatc tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt
     6301 tgctcaatag atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg
     6361 taccatttaa ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga
     6421 tttttttgtc ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa
     6481 tcgtatctcc tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt
     6541 ttagctgact atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg
     6601 tgattccagc ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta
     6661 catcgtccac caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag
     6721 aagtagctgt atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc
     6781 atcctgtcgc taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt
     6841 ttagttcagt tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc
     6901 cccactgact atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct
     6961 ccaatacctt taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca
     7021 cgatattggc tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc
     7081 cttgtgtaac gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca
     7141 actgatcaaa acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg
     7201 gaaggttctg gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc
     7261 ctgttgaatc acttgttgag atgtaactat tccacgtatt tatctctgca cttgcagaaa
     7321 atgcatatgc tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag
     7381 ctggagctaa aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat
     7441 caacagaaaa aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt
     7501 tgtttggact cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa
     7561 catcaaattg aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa
     7621 tatctgccaa tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc
     7681 ctgctgataa taaactaccg ctagtaacct tgatgggggc accggcaata tacaagtagg
     7741 cgttgttcca gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga
     7801 tgatcgccac aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta
     7861 gtttcacatg gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac
     7921 catcgttcaa ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa
     7981 gcttcccgcc attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt
     8041 atggatcata agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga
     8101 taaatatact taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat
     8161 tttgtagcaa catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga
     8221 agccataatt ctataccaat gatcaaataa agaaaacgga tcttttcttc attcgtctcc
     8281 gggaatacgc catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat
     8341 gcgccattga ctgaaccact tggatgcgga actggactta gatcataaaa tgtaatcgta
     8401 cctgaacttt tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca
     8461 ataatccctc ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc
     8521 ttttttcttt ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc
     8581 agcaaccaat cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt
     8641 ttcatttact ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc
     8701 attactgtcg cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc
     8761 accagctaaa tcaagattca agtgaaaatt cgaatttggt gcaactaaaa attcattaga
     8821 cgtattttca atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct
     8881 gataatccta ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc
     8941 gtccaaatct cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa
     9001 aacgactgcg agattataag caatacgatt tttgatttgt gctccttctg tagcattttc
     9061 tgtttcagct tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct
     9121 ttttgctggg attttcactt ctataggtat agttgtttct gcatgtgctg atacattgat
     9181 ttcctttttc gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt
     9241 tgggtttttt tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga
     9301 gacctcaaat gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt
     9361 ttgttccggt gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg
     9421 aaataccggt ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa
     9481 aatgaaaaat agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat
     9541 tgtccaccac atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc
     9601 accgcagttt gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa
     9661 gtcaaatcag ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct
     9721 ggatcataaa cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt
     9781 gtcgctgcac ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg
     9841 ttatctgacg tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact
     9901 tgtgaaccgt ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca
     9961 tctgcaccta cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg
    10021 tctacttgat acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcagga
    10081 actacatcca atgttaaaga acctgtctgg ccagttgctg gattttccgg atctgctggc
    10141 tctactggtt ttgaaggatc agtagggtta agaggctcaa cggtatttgt tggttcagta
    10201 aaggtaatat ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg
    10261 cctagaagaa gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac
    10321 tcctttttct tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc
    10381 atccgcttac ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt
    10441 ttacatgaat attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc
    10501 gtttcaaatt attctattag tgacatcctt gaactataaa aataggagta cggcatcatc
    10561 tgccgcactc ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt
    10621 tatttttttg atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc
    10681 tgttttaaag aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata
    10741 aataacgggt ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg
    10801 ttgtccacgt cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc
    10861 cacatattgc aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt
    10921 agatacccaa attccaccag caccagattc taatgatcga tagacatctt cttctgattg
    10981 aacacctttc acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg
    11041 actaagtttt tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg
    11101 ataagcctga acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc
    11161 acggtttccg ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt
    11221 ggccacttca agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca
    11281 ttgcggtgct ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga
    11341 acttgctgta tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt
    11401 cacatgagct aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc
    11461 ttcgtcaaaa tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat
    11521 caaacgatga ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact
    11581 gctgatgtaa ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc
    11641 catattgata aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc
    11701 aatgccttct ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat
    11761 attactcttc gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt
    11821 cactactctg gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca
    11881 aaagtagtgt caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta
    11941 ttaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc
    12001 gaaactaagc gactttttca caacctctcg ataaaacggt gttcaatcag ctgaccctcg
    12061 gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac
    12121 tcaggtcaga acgttgactt cacagtctct tccttaaacg gtgttcaaaa acttgcatct
    12181 gcggtaataa gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta
    12241 ttacttgatg cagggcaggt ttttcacgac ctctgattca cggtgtttct ttttcatcaa
    12301 catttttacg aaatactgtg gaagactatt gaaataagtg tggattgttt ctttgtgttt
    12361 caatcgtttc ttgccattct tcatttaatt ttggattacc gttttcttcc ttgaactcat
    12421 aaggtcctaa ttcaaaaatc ccagacagta tttgattcac gatttcatat agttcagtgt
    12481 gatcagacac atgattcttg aagatcatct cattatcacc acgtttgaac ctcacattcg
    12541 ggctctcatg atgaacctct atgcattttt ctcctttttt taactgacgg acttctgaaa
    12601 acatctcata aatatcttct ccagtaaaac cttcagcaag cgccagactg tccacaagac
    12661 cgcctactgt cagttcagag attattgctt gattggaatc taattttaat gtatagcttg
    12721 tcgcttctac tacgccacat gcattgcaac cgccttctga tagttcgctt tttctaatca
    12781 attgaacttc tttcattttc tcttcctcct cttaattttc tacttatgta tttttatcac
    12841 ctgacatagg tgactcctat caacgctatc atgtgctttt ttcctcagaa atcctattta
    12901 tccattccat ctatccctta ttcttctact tttctttctc tagcatataa gagatacgca
    12961 tagtattcaa gcaaggattg tcgatataaa aagaacaaat actgctattt tgatttcttt
    13021 tggctatatt acatataaat aaaatttatc atacctgctc tttttgacta tatcatcatc
    13081 tgacttctat acttcgtttg tattaaaact taggaggaat cattcatgct tacagggtta
    13141 aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca
    13201 aaatatggaa tcactggtat gcttccaagt actgtccaaa cactggaaca acaatctgtt
    13261 caagcttatg gtcaatactt aagcaaacaa acagatttgg aaaaacgtat cttcttaatg
    13321 aatctattca atacgaatcg gacattgttt tataaattga tgggcgaaca tttagttgaa
    13381 ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt
    13441 ttcttgaaac cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa catcaaagca
    13501 agtttgaaga acgcggcaga tggacgagac attcgtttga ttgttgtgac tgatgcagaa
    13561 ggaatcttag gtatgggcga ctggggtgtg aacggtgttg acatcgctat cggcaaattg
    13621 atggtctata ctgctgctgc tggtattaat ccagcacaag ttttgcctgt atcgatcgac
    13681 gctggtacta ataatgaaga attattgaat aatcccttat acttaggcaa tcgtcatgct
    13741 cgtgtagaag gcgaaactta ctatgaattc atcgatcaat tcgttcaatc agctaccgaa
    13801 ttattcccag aattgttatt gcattgggaa gattttggac gaggaaatgc tgcagcgatc
    13861 ttagaaaaat acgaagatca aatcactact ttcaacgacg atatccaagg tacaggaatt
    13921 gttgtcctag caggtgtatt aggcggattg aatatatccg gtgaacaatt gaaagatcaa
    13981 ataattttga catttggtgc tggtactgct ggtgtgggta tcgcaaacat ccttttagat
    14041 gagatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat
    14101 aaacaaggtt tattgtttga agatacagaa ggattgactc ctggtcaaat cccattcgct
    14161 cgcaaacgct cagatttcac taatgctgat gaattgacaa acttagaagc agttgtaaaa
    14221 gcgatccatc cgacaatcat gattggtact tctacccaac ctggagcatt cactgaagaa
    14281 atcgtgaaag aaatggctgc tcatacacca cgtccgatca tcatgccttt gtctaatcca
    14341 acaaaattgg cagaagcaaa agcaaaagat ttaatagaat ggacggacgg aaaagcatta
    14401 gttggaacag gtatccctgc tgctgatgtt gaatataatg gtgtaactta ccaaatcggt
    14461 caagcaaata atgctttgat gtacccaggt ttaggacttg gtttgattgc ttcaacagct
    14521 acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat
    14581 gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga attttctcaa
    14641 acgattgctg aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg tgaagaaatc
    14701 acggatatca aagaagcagt tgaatctgct aaatgggttc ctgaatataa atctttagaa
    14761 gattaagatc gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa
    14821 aagcgactgc ggcaaaacag cggccagcca actcctgctc ttttgtcgct tttctttggc
    14881 ttttttcata tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaact
    14941 gcaactgcag ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat caccggcgta
    15001 agtttgccac tttatttgat catggtcctt gtcctgatca ttgcaatggc tttagggaaa
    15061 ttaccgacga atatgattgg accaatcgca gttttggtta ttttcggtaa tttatttcat
    15121 tttatcggga caaaaatccc tatcgtcaaa agttatttgg gtggtggttc cgtttttgcg
    15181 atcttcgctt ctgctgcgat tgctacattt gggatcttac ctgaatatgt agtgaaatcc
    15241 acagaaaact ttgtcagcaa tatgggcttt tgggactttt atattgctgc cttgattact
    15301 ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc
    15361 gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtggg catgcttatc
    15421 ggaaatggtt ttgcaaattc cgttttatac atctcattac ctgcgatggc tggtggtgtt
    15481 ggagccggag ccgttccttt atcaacgatt tatgcaaatg ttttaggtac ggatgcttct
    15541 tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt
    15601 gcagcattag ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa tggtaaatta
    15661 atggaaaaag gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc cattccacag
    15721 ttaggtgttg gtttgatggt atcattgact ttcttcatct taggacaaat tggtaactat
    15781 tttgttccta aagtccatgc ttacgcattt atgattatta tcgttgtcat ctgtaaaatc
    15841 gccaacctat taccagagta ttatgaagat gcagctatca tgttcaataa cttgattgtc
    15901 aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttgctgaa tctgaatgta
    15961 ttagcttctg ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt
    16021 tcagttgttt caggatttgt cggtcgtttg tttggtttat atccaattga atcaactatt
    16081 actgctggct tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt cttatctgca
    16141 gctaatcgga tggaacttat tgcttttgct caaatgggca atcgacttgg tggtgccatc
    16201 gttttgatca tctctggttt cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac
    16261 ctgatcatca agtatcggct tggtgatcgg gtattttttt gataaagatt tacaggattt
    16321 tcaaaaatct tttttgcatt ttatcggaat gataaccgat gtgttcataa aattggtgtg
    16381 cttctactct gtattctgca gaattaagcc ttattgtcgt tattcctcgt tttttcgctt
    16441 cttcttctac gccagtcatc aaagcctttc cgatgcctct tttttcagca tatttagaca
    16501 cagcaagtgc cataatattg aacattggat cgctaaaaat cgattcatag gactccgctt
    16561 gtacatagcc agctgctttt ttagaagtag gatcttcata aaccaagaaa aaatttttct
    16621 ctggctcctt cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt atatcatacc
    16681 ctaattgatc cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc atttcccgta
    16741 acatccaaat caccagccaa tttctgattt tttaaaatga atttttttat catagtatac
    16801 tctttttttc atttagaaca atatgtttca gtgattttga cttgcgttag tctagattct
    16861 cctaatgttt gtaagatgac aaaggcattg taacaattga ccaaaagaac cacgaggaca
    16921 attacttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
    16981 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
    17041 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
    17101 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
    17161 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
    17221 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
    17281 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
    17341 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
    17401 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
    17461 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
    17521 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
    17581 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
    17641 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
    17701 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
    17761 agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    17821 aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag atgcaagctt
    17881 ttgaacaccg tttaagaaag aggtcgtggg cctgccgttc agctccgagt cacaa
//
LOCUS       DAXWLB010000048        17667 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXWLB010000048 DAXWLB010000000
VERSION     DAXWLB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.048"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..839
                     /locus_tag="ACGE4H_001252"
     CDS             <1..839
                     /locus_tag="ACGE4H_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8364681.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1192..2040)
                     /locus_tag="ACGE4H_001253"
                     /pseudo
     CDS             complement(1192..2040)
                     /locus_tag="ACGE4H_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2193..3305
                     /locus_tag="ACGE4H_001254"
     CDS             2193..3305
                     /locus_tag="ACGE4H_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFF8364682.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ACGE4H_001255"
     CDS             complement(3383..3676)
                     /locus_tag="ACGE4H_001255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFF8364683.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ACGE4H_001256"
     CDS             3844..4158
                     /locus_tag="ACGE4H_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFF8364684.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ACGE4H_001257"
     CDS             4179..6158
                     /locus_tag="ACGE4H_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFF8364685.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ACGE4H_001258"
     CDS             6171..6497
                     /locus_tag="ACGE4H_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFF8364686.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ACGE4H_001259"
     CDS             6513..7031
                     /locus_tag="ACGE4H_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364687.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVAWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ACGE4H_001260"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ACGE4H_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFF8364688.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ACGE4H_001261"
     CDS             complement(8467..9972)
                     /locus_tag="ACGE4H_001261"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFF8364689.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ACGE4H_001262"
     CDS             complement(10064..10654)
                     /locus_tag="ACGE4H_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8364690.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ACGE4H_001263"
     CDS             10833..12479
                     /locus_tag="ACGE4H_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFF8364691.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ACGE4H_001264"
     CDS             12528..13247
                     /locus_tag="ACGE4H_001264"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8364692.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ACGE4H_001265"
     CDS             13726..14673
                     /locus_tag="ACGE4H_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364693.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ACGE4H_001266"
     CDS             14685..15605
                     /locus_tag="ACGE4H_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364694.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ACGE4H_001267"
     CDS             15646..17103
                     /locus_tag="ACGE4H_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364695.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..>17667
                     /locus_tag="ACGE4H_001268"
     CDS             17448..>17667
                     /locus_tag="ACGE4H_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8364696.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRG"
BASE COUNT         5556 a         3024 c         3604 g         5483 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tatgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tgcatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttcttg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggca
//
LOCUS       DAXWLB010000049        17376 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXWLB010000049 DAXWLB010000000
VERSION     DAXWLB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.049"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            229..1995
                     /locus_tag="ACGE4H_001269"
     CDS             229..1995
                     /locus_tag="ACGE4H_001269"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFF8364697.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2097..3356
                     /locus_tag="ACGE4H_001270"
     CDS             2097..3356
                     /locus_tag="ACGE4H_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8364698.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3503..4804
                     /locus_tag="ACGE4H_001271"
     CDS             3503..4804
                     /locus_tag="ACGE4H_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8364699.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4807..5658
                     /locus_tag="ACGE4H_001272"
     CDS             4807..5658
                     /locus_tag="ACGE4H_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFF8364700.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5671..6486
                     /locus_tag="ACGE4H_001273"
     CDS             5671..6486
                     /locus_tag="ACGE4H_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFF8364701.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6637..7803
                     /locus_tag="ACGE4H_001274"
     CDS             6637..7803
                     /locus_tag="ACGE4H_001274"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8364702.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8033..8680
                     /locus_tag="ACGE4H_001275"
     CDS             8033..8680
                     /locus_tag="ACGE4H_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFF8364703.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8764..9708
                     /locus_tag="ACGE4H_001276"
     CDS             8764..9708
                     /locus_tag="ACGE4H_001276"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFF8364704.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9843..11294
                     /locus_tag="ACGE4H_001277"
     CDS             9843..11294
                     /locus_tag="ACGE4H_001277"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFF8364705.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11315..12151
                     /locus_tag="ACGE4H_001278"
     CDS             11315..12151
                     /locus_tag="ACGE4H_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8364706.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12151..12810
                     /locus_tag="ACGE4H_001279"
     CDS             12151..12810
                     /locus_tag="ACGE4H_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8364707.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12936..13625
                     /locus_tag="ACGE4H_001280"
     CDS             12936..13625
                     /locus_tag="ACGE4H_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364708.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13631..14587
                     /locus_tag="ACGE4H_001281"
     CDS             13631..14587
                     /locus_tag="ACGE4H_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFF8364709.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14631..15143
                     /locus_tag="ACGE4H_001282"
     CDS             14631..15143
                     /locus_tag="ACGE4H_001282"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364710.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15399..15947)
                     /locus_tag="ACGE4H_001283"
     CDS             complement(15399..15947)
                     /locus_tag="ACGE4H_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFF8364711.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16036..17217)
                     /locus_tag="ACGE4H_001284"
     CDS             complement(16036..17217)
                     /locus_tag="ACGE4H_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFF8364712.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         5717 a         3088 c         3480 g         5091 t
ORIGIN      
        1 actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag
       61 gaagtagatt aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa
      121 aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgtttct
      181 ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct
      241 gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt
      301 attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctttg tggagatcca
      361 tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt
      421 accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaaggcg cttacagtat
      481 gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt
      541 ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat
      601 caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt
      661 cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga
      721 agtaaagatc cagaccgaga agacttcttc gggggagatt tgcaaggtgt gagggatcat
      781 ttggattact tgacggacct tggcgtcaat ggcatctatt tttgtcccat atttaaagcc
      841 acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat
      901 aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc
      961 gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac
     1021 ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat
     1081 cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact
     1141 ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc
     1201 gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa
     1261 gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc
     1321 tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat
     1381 gcagtgatga attatgcatt cacagattcg attaaagatt attttgcaaa aaagaaaatc
     1441 acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg
     1501 aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt gaccctttgc
     1561 caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct
     1621 ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc
     1681 aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa
     1741 cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta
     1801 atagtatatg atcgtgaaga taagctctat ttcacacggg aactagaggg acagatcatc
     1861 tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaggtgatt
     1921 cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt
     1981 ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt
     2041 tgtgcccatt ataataaggg tgtaacagca cgttgaatac acaggaggaa atgaagatga
     2101 aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat
     2161 tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt
     2221 taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg
     2281 aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat
     2341 cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc
     2401 ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag
     2461 cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg
     2521 cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa
     2581 cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag
     2641 gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg
     2701 caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga
     2761 ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc
     2821 caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca
     2881 ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata
     2941 tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg
     3001 gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat
     3061 ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa
     3121 tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag
     3181 ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag
     3241 tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag
     3301 aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa
     3361 aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga
     3421 gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag
     3481 caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa
     3541 aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa
     3601 aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt
     3661 tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt
     3721 tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg
     3781 tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa
     3841 tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac
     3901 acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt
     3961 cactgcattt actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt
     4021 aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc
     4081 agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg
     4141 tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat
     4201 cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat
     4261 attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc
     4321 ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat
     4381 tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt
     4441 acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca
     4501 gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac
     4561 acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca atggtggtcc
     4621 cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact
     4681 tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt
     4741 catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga
     4801 ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt
     4861 acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca
     4921 agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg
     4981 actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat
     5041 acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc
     5101 tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg
     5161 attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta
     5221 gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg
     5281 aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct
     5341 aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa
     5401 ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta
     5461 gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt
     5521 atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca
     5581 ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct
     5641 ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc
     5701 gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt
     5761 ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc
     5821 aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa
     5881 caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa
     5941 caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa
     6001 tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat
     6061 aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta
     6121 cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca
     6181 acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc
     6241 attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt
     6301 catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca
     6361 gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg
     6421 ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc
     6481 gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta
     6541 gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat
     6601 taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat
     6661 caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg
     6721 aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc
     6781 ccaatgaaag agattacaag taacgcttct ttggcactag gagaagccta tatggatcat
     6841 cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac
     6901 agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa
     6961 caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt
     7021 tggctagatc caactttgac ttattcttgt gcttatttca atacacctga agatacgttg
     7081 gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc
     7141 gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag
     7201 tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa
     7261 agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa
     7321 ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct
     7381 gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct
     7441 ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat
     7501 attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac
     7561 gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat
     7621 tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc
     7681 tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc
     7741 gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca
     7801 taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct
     7861 ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc
     7921 tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt
     7981 cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa
     8041 aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac
     8101 caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc
     8161 ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc
     8221 aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa
     8281 atacccagat actgcaatta ctagtttgga attagatagt gattggggat cttacttcta
     8341 caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga
     8401 aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa
     8461 aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc
     8521 cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac
     8581 tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag
     8641 ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag
     8701 agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt
     8761 tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca
     8821 tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga
     8881 gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat
     8941 tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat
     9001 tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt
     9061 ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa
     9121 agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca
     9181 caactagctt acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt
     9241 tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg
     9301 gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat
     9361 ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc
     9421 ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca
     9481 cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca
     9541 tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtacagagct tcataaagaa
     9601 gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga
     9661 tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa
     9721 ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa
     9781 gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata
     9841 atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag
     9901 aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg
     9961 tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag
    10021 ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc
    10081 tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg
    10141 tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg
    10201 gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg
    10261 aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc
    10321 ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat
    10381 tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat
    10441 ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat
    10501 tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc
    10561 aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact
    10621 cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag
    10681 aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt
    10741 attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc
    10801 agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc
    10861 agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg
    10921 tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg
    10981 cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca
    11041 gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga
    11101 agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta
    11161 ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag
    11221 aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa
    11281 atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct
    11341 aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga
    11401 taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact
    11461 gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc
    11521 aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg
    11581 acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac
    11641 aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat
    11701 gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa
    11761 agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt
    11821 agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac
    11881 agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat
    11941 ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa
    12001 agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga
    12061 tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa
    12121 agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc
    12181 atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc
    12241 gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat
    12301 tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca
    12361 ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct
    12421 tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca
    12481 ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt
    12541 ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt
    12601 aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa
    12661 atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt
    12721 ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta
    12781 gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca
    12841 ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa
    12901 aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa
    12961 tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca
    13021 cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa
    13081 gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca
    13141 aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag
    13201 taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag
    13261 aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg
    13321 ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa
    13381 aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat
    13441 tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt
    13501 tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc
    13561 atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg
    13621 agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa
    13681 agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg
    13741 tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc
    13801 tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg
    13861 gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag
    13921 tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga
    13981 tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag
    14041 tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag
    14101 aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt
    14161 tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt
    14221 cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg
    14281 caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt
    14341 gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact
    14401 cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg
    14461 actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct
    14521 agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat
    14581 ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc
    14641 ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt
    14701 tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga
    14761 ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg
    14821 gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga
    14881 aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag
    14941 gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata
    15001 tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt
    15061 ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag
    15121 catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca
    15181 ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa
    15241 aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt
    15301 acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac
    15361 gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt
    15421 attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata
    15481 gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc
    15541 tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta
    15601 ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct
    15661 gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa
    15721 ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga
    15781 ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt
    15841 gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa
    15901 gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt catttctcct
    15961 ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact
    16021 ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa
    16081 agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt
    16141 taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag
    16201 gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc
    16261 tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac
    16321 tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat
    16381 ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc
    16441 aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta
    16501 ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc
    16561 tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt
    16621 caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat
    16681 cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag
    16741 gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc
    16801 tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac
    16861 agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat
    16921 aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc
    16981 ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc
    17041 caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag
    17101 ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat
    17161 caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat aactcaaata
    17221 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
    17281 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
    17341 gcgaacaaaa taaaaggttt aaaaagtgta acgtct
//
LOCUS       DAXWLB010000050        16808 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXWLB010000050 DAXWLB010000000
VERSION     DAXWLB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.050"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            163..1104
                     /locus_tag="ACGE4H_001285"
     CDS             163..1104
                     /locus_tag="ACGE4H_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFF8364713.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFIALIK"
     gene            1173..1904
                     /locus_tag="ACGE4H_001286"
     CDS             1173..1904
                     /locus_tag="ACGE4H_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8364714.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="ACGE4H_001287"
     CDS             2048..4228
                     /locus_tag="ACGE4H_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFF8364715.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="ACGE4H_001288"
     CDS             4248..4730
                     /locus_tag="ACGE4H_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFF8364716.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="ACGE4H_001289"
     CDS             4746..5507
                     /locus_tag="ACGE4H_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFF8364717.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /gene="murC"
                     /locus_tag="ACGE4H_001290"
     CDS             5638..6972
                     /gene="murC"
                     /locus_tag="ACGE4H_001290"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFF8364718.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="ACGE4H_001291"
     CDS             7279..7764
                     /locus_tag="ACGE4H_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFF8364719.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7757..8440
                     /locus_tag="ACGE4H_001292"
     CDS             7757..8440
                     /locus_tag="ACGE4H_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFF8364720.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8566..11211
                     /gene="polA"
                     /locus_tag="ACGE4H_001293"
     CDS             8566..11211
                     /gene="polA"
                     /locus_tag="ACGE4H_001293"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFF8364721.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11256..12092
                     /gene="mutM"
                     /locus_tag="ACGE4H_001294"
     CDS             11256..12092
                     /gene="mutM"
                     /locus_tag="ACGE4H_001294"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFF8364722.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12056..12685
                     /gene="coaE"
                     /locus_tag="ACGE4H_001295"
     CDS             12056..12685
                     /gene="coaE"
                     /locus_tag="ACGE4H_001295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFF8364723.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12737..13069
                     /locus_tag="ACGE4H_001296"
     CDS             12737..13069
                     /locus_tag="ACGE4H_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8364724.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13381..13872
                     /gene="nrdR"
                     /locus_tag="ACGE4H_001297"
     CDS             13381..13872
                     /gene="nrdR"
                     /locus_tag="ACGE4H_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFF8364725.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13895..15268
                     /locus_tag="ACGE4H_001298"
     CDS             13895..15268
                     /locus_tag="ACGE4H_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFF8364726.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15268..16194
                     /gene="dnaI"
                     /locus_tag="ACGE4H_001299"
     CDS             15268..16194
                     /gene="dnaI"
                     /locus_tag="ACGE4H_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFF8364727.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16323..16763
                     /locus_tag="ACGE4H_001300"
     CDS             16323..16763
                     /locus_tag="ACGE4H_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFF8364728.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5737 a         2950 c         3700 g         4421 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttatag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     8521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     8581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     8641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     8701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     8761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     8821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     8881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     8941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     9001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     9061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     9121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     9181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     9241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     9301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     9361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     9421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     9481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     9541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     9601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     9661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     9721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     9781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     9841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     9901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     9961 ttgagccaag aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca
    10021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
    10081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
    10141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
    10201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
    10261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
    10321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
    10381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
    10441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
    10501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
    10561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
    10621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
    10681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
    10741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
    10801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
    10861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
    10921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
    10981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
    11041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
    11101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
    11161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
    11221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
    11281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
    11341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
    11401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
    11461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
    11521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
    11581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
    11641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
    11701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
    11761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
    11821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
    11881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
    11941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
    12001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
    12061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
    12121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
    12181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
    12241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
    12301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
    12361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
    12421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
    12481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
    12541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
    12601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
    12661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
    12721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
    12781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
    12841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
    12901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
    12961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat
    13021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    13081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
    13141 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
    13201 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
    13261 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
    13321 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
    13381 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
    13441 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
    13501 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
    13561 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
    13621 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
    13681 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
    13741 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
    13801 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
    13861 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
    13921 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
    13981 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
    14041 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
    14101 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
    14161 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
    14221 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
    14281 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
    14341 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
    14401 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
    14461 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
    14521 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
    14581 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
    14641 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
    14701 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
    14761 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
    14821 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
    14881 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
    14941 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
    15001 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
    15061 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
    15121 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
    15181 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
    15241 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
    15301 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
    15361 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
    15421 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
    15481 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
    15541 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
    15601 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
    15661 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
    15721 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
    15781 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
    15841 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
    15901 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
    15961 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
    16021 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
    16081 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
    16141 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
    16201 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
    16261 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
    16321 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
    16381 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
    16441 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatca
    16501 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
    16561 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
    16621 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
    16681 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
    16741 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
    16801 tatagaac
//
LOCUS       DAXWLB010000051        16723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXWLB010000051 DAXWLB010000000
VERSION     DAXWLB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.051"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(46..780)
                     /locus_tag="ACGE4H_001301"
     CDS             complement(46..780)
                     /locus_tag="ACGE4H_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFF8364729.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="ACGE4H_001302"
     CDS             1106..1516
                     /locus_tag="ACGE4H_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFF8364730.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="ACGE4H_001303"
     CDS             1509..2210
                     /locus_tag="ACGE4H_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFF8364731.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3823
                     /locus_tag="ACGE4H_001304"
     CDS             2210..3823
                     /locus_tag="ACGE4H_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364732.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3813..4034
                     /locus_tag="ACGE4H_001305"
     CDS             3813..4034
                     /locus_tag="ACGE4H_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFF8364733.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="ACGE4H_001306"
     CDS             4031..4792
                     /locus_tag="ACGE4H_001306"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFF8364734.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5173..5682
                     /locus_tag="ACGE4H_001307"
     CDS             5173..5682
                     /locus_tag="ACGE4H_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFF8364735.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5752..6291)
                     /locus_tag="ACGE4H_001308"
     CDS             complement(5752..6291)
                     /locus_tag="ACGE4H_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8364736.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE4H_001309"
     CDS             complement(6431..7042)
                     /gene="rpsD"
                     /locus_tag="ACGE4H_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFF8364737.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7304..8155
                     /locus_tag="ACGE4H_001310"
     CDS             7304..8155
                     /locus_tag="ACGE4H_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFF8364738.1"
                     /translation="MRGHVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPAATILVEGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLAYFASQGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8183..8818
                     /locus_tag="ACGE4H_001311"
     CDS             8183..8818
                     /locus_tag="ACGE4H_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFF8364739.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8838..9611
                     /locus_tag="ACGE4H_001312"
     CDS             8838..9611
                     /locus_tag="ACGE4H_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8364740.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10019..10816
                     /locus_tag="ACGE4H_001313"
     CDS             10019..10816
                     /locus_tag="ACGE4H_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFF8364741.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10794..11207
                     /locus_tag="ACGE4H_001314"
     CDS             10794..11207
                     /locus_tag="ACGE4H_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8364742.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE4H_001315"
     CDS             11191..13836
                     /gene="mutS"
                     /locus_tag="ACGE4H_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFF8364743.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE4H_001316"
     CDS             13854..15962
                     /gene="mutL"
                     /locus_tag="ACGE4H_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFF8364744.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15984..16544
                     /locus_tag="ACGE4H_001317"
     CDS             15984..16544
                     /locus_tag="ACGE4H_001317"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFF8364745.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5429 a         2989 c         3620 g         4685 t
ORIGIN      
        1 cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag ccataaataa
       61 taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa ctggtaccca
      121 tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt gtacaaaacg
      181 tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa gtgaagcaac
      241 taatgtccat acaaggaaac cgagtgctaa gtgagctgta ccaagattat cttggaagaa
      301 tggtgctttt gtaataccta aggcaccaaa gaataaaacg aatgaaccaa agaattcatt
      361 gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac ctaacacatg
      421 gttcacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga tcaattgtcc
      481 aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc aagggaatag
      541 accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc tgattccacc
      601 aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca gcatccagtc
      661 actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt tacctagaac
      721 gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg agtaatccat
      781 aaatacctcc aaaagttata aaaatgcaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaacca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagctatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agagggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc aggaagggta tctgtaaacg aaatggagga
     1501 aaatcaaatt gactgagttt tttgtgaatc cactatttgg cttaagtttg actttaggag
     1561 tgtatgtatt atctgataaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat cggtacagca aaagcaatcg
     2101 agattggcga aatagaagga atcatttccg gacttgcgat atgcgtaaat gggatattga
     2161 ccgttttgtt gctgccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatacatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaagtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg ggtcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgaccgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cagtgacacc ttacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaactc tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaggat cgttttggtg aagttacgat tttactgaat acatcgggga tattggatgc
     4321 ctacaaacca ttgatggaaa ctgacgaatc gctgtgggat cttatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc gaaaaagcgg
     4441 aacaatcatc aacatggctt cgattgcggg tttggttgct ggcggaggtg ggatcgctta
     4501 tacaagtgcg aaacatgcaa tcgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggctga gtgggtcgca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga tggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaagaa
     4861 agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat tctttttaat
     4921 aatcgttaat attcgaaact aagcggcttt tcacgacctt ttgatataaa cggtgttcaa
     4981 aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc tatttttatt
     5041 tgactattct tattacttgt tgcagggcag ctttttcact accttctgcg gcacttcagt
     5101 tggaattttc ttcccatcct ttggtcgcag tcattaccaa gataaaatta tcttgcaaag
     5161 gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac aacgacagat
     5221 gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt tttgtctgtc
     5281 tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct gtccttgtat
     5341 aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat ggcttctcct
     5401 cttggactga tagatgttgt agtaggaagc gtcagcacgc tgcttgtatt gcttatcaac
     5461 tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggctgtgac agcatgcgta
     5521 tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca actacctttt
     5581 ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt cggtgggata
     5641 attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt ttaatttaaa
     5701 atatggcagc gtggaagaga ctttgagtct tcttccatgc tgcttttttg attaatttgt
     5761 atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg ctccagttgc
     5821 cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag ttgtaaaagt
     5881 aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa gagagcccat
     5941 tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca tccaaccagt
     6001 tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa ataaaacagc
     6061 tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta aaagataaac
     6121 tacaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc caatagcaaa
     6181 tgtttgcccc gtcaaaggaa ccaagccaaa aggaatggtc atctgagaaa ggacagaaat
     6241 aatcgcggca aattccgcag caagtattaa ttctttcaat gttcggttca taatgccctc
     6301 ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag tgattacttt
     6361 gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt tttcaagaag
     6421 gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg tcgatttctg
     6481 gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt tcagcgtcaa
     6541 agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact gttacgatgt
     6601 tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat gaagggatat
     6661 caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt gcttgacgac
     6721 gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct agtaagatca
     6781 tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac aagttacgga
     6841 attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt aattgcatgc
     6901 cgtattctga ttgtttgcca cggctgttag gtccatgttg acctggtgcg taagggcgac
     6961 gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa attttccatg
     7021 atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt ttggagtaaa
     7081 ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac ctttgcagcc
     7141 gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta ttatttttct
     7201 gctgcattat tttacacaaa ctacagtata tgatagatca tccacgaatg tcaagataat
     7261 tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag ggcatgtgca
     7321 aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat caccattatt
     7381 tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca gcttttcacg
     7441 ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg ctgtggcatt
     7501 tgcaatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt tatacgcatt
     7561 tatgttcagc tggggtctgg tcatgattct atacatgaac gctgaattag gaacatcgaa
     7621 tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta aggcagccaa
     7681 gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg gatttcttat
     7741 ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag aatcaatagc
     7801 agtaaaacta gcaaaacctg cagcaacgat tttggtggaa gggatgtttg cgaatatcgt
     7861 tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgccggga aagtgatcac
     7921 agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga tagcgaattt
     7981 tcctgccttt acgcttgctt actttgcttc tcaaggacag acggatggaa tgacagtcag
     8041 caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg gcggtttagt
     8101 aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg actaaaaagt
     8161 cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg aggaaaaagc
     8221 gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac ctaagccgga
     8281 acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta tttctgaagt
     8341 tttagctttt gtttattatt attatgaaca aatcgaaaga gagtatcagt atgtagcgat
     8401 cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg tattgttgcc
     8461 actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag tatacagcac
     8521 atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg aaaaaaagga
     8581 agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac agcaaacaga
     8641 cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat tctttatcca
     8701 aaaagcaatt ggttgggcat tacgcgatta cagtaagacc gatgccgatt gggtaaaaag
     8761 acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt atttataaga
     8821 aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat ggaacgttgt
     8881 tagattcaaa caaacaagct ttagatagca cgagagaagc attggaaaaa ttacgcaaag
     8941 ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa gagatcattc
     9001 gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt ttagaccatg
     9061 aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt caagaagcgc
     9121 atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt tcttcttcca
     9181 atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg cctgaattag
     9241 atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc gatcaaacat
     9301 atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat gagaacagtg
     9361 tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta gcagaacaag
     9421 taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac cgtgaaatgt
     9481 tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta caagcagaag
     9541 ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg aaagagttaa
     9601 gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag cccagccaaa
     9661 caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat gcaagctttt
     9721 gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa taagaaggga
     9781 tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga agaagtagat
     9841 gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag tcccattttt
     9901 ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt tgaccttttc
     9961 tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg aatttagtgt
    10021 gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga ttactgaata
    10081 tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg gtgagaatgc
    10141 cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag acggcgtaga
    10201 tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt ttgtaaatga
    10261 agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac aagggatggt
    10321 ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc gttcttttat
    10381 ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag ccagaaaacg
    10441 tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac aagcaatggg
    10501 ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg ttcagacaaa
    10561 tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga tgacaggtcc
    10621 ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc tgaccgcatt
    10681 accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct gtatcttgga
    10741 attggatgat acgacaggtc atgcaaaaaa aatccagctg attcagatca acgatgatcg
    10801 accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa attcaaaaag
    10861 aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc tacaaagaac
    10921 ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa atcaaacaag
    10981 cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa caagaagcaa
    11041 tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt gtagcttata
    11101 gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg atacaagaag
    11161 aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa tactcctatg
    11221 atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt ctatcgttta
    11281 ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct tttggaatta
    11341 acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg tgtacctcac
    11401 catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt agcgatttgc
    11461 gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt gatccagcta
    11521 gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa caatttttta
    11581 acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt aagtacggga
    11641 gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc ctctgcattg
    11701 cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga acagctaagc
    11761 gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc ggaatttagc
    11821 tttttgacaa gcgaactagt cgatccgctg aaaaaagaag caaccggcaa actattgact
    11881 tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt agaataccaa
    11941 ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt aagccaatct
    12001 attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac aaaaacagca
    12061 atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc aaaacaaatt
    12121 aaagcaagac aagaaatggt cgcttctttg ttagacagct attttgaacg tatcgatttg
    12181 cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt agctttcggt
    12241 aatgtcaatg ggcgagactt gatccagctg aaaaattctt tagaacaagt gccaatgatc
    12301 agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc agaaatgcag
    12361 ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc gccgttacaa
    12421 ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat gtaccgttct
    12481 gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg tcaagaaaca
    12541 ggaatcaaaa acttaaaaat cggatttaac cgcgttttcg gttattatat agaaatcaca
    12601 aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac tttagcaaat
    12661 gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct cgaagcagaa
    12721 gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga agtcaaaaag
    12781 gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt gctacaaagt
    12841 tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag tgacaagcat
    12901 cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg acatcaggaa
    12961 tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat tacaggaccg
    13021 aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct gatggcgcaa
    13081 atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg gatttttacc
    13141 cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt agagatgatg
    13201 gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt tgacgaacta
    13261 gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat cgaatatatc
    13321 catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact gacggtactt
    13381 gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa agacggagaa
    13441 gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg catacatgtg
    13501 gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat cctgcatacg
    13561 ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga gaaaactaaa
    13621 acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga ccatatacaa
    13681 gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc cgaaaatgaa
    13741 acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc aatggatgca
    13801 ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata tcgatggcca
    13861 aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa gtagtggagc
    13921 gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc agtactcaga
    13981 ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat aacggagaag
    14041 gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt aagatccata
    14101 atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct ttgccaagta
    14161 ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa caaggcacct
    14221 atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg cgtcaaggaa
    14281 cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa tacgtgaaaa
    14341 cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca ttgagtcatc
    14401 catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact gctggaaatg
    14461 gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa aaaatgctga
    14521 aaatcgaagg aaaagatcta gattttgagt tatttggcta tgtttcattg cctgaggtga
    14581 ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc aagaactttt
    14641 ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt cgtttcccta
    14701 ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt catccgacga
    14761 agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt aacgccattc
    14821 aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta cggtttaaga
    14881 aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc tacgcttttg
    14941 aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgagagaca ggtacctttt
    15001 tcgtagaaga accaaagaac acatttgttg acagaattgt tgataagtct gtggaaaatg
    15061 ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa acagcgaaaa
    15121 cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg gaaaaatctg
    15181 aaaagaaaga atttcccgtt gtggataaca tggagaaaac aaatgaagaa caagcagata
    15241 aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat cctgaatttg
    15301 atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta gaaacggaaa
    15361 aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga acgtatcttt
    15421 ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa gagcgcatta
    15481 agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa gaattgcttg
    15541 ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag caaaaacact
    15601 tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt attgtacggg
    15661 cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag atgatcgata
    15721 tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca gcaatcatga
    15781 tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa gcacgtgtgc
    15841 tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc cgtccagtat
    15901 tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa gacccgcatt
    15961 aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca ccaagaagaa
    16021 aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat atcgatgaaa
    16081 cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa gaaaaagcac
    16141 gaattgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt gacacgactg
    16201 ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct gaattcatgc
    16261 tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta aaaacaaagg
    16321 aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg acagacgaag
    16381 agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca tacggcatcc
    16441 aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt atcgtaggat
    16501 ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa aagcggacta
    16561 tctgattttc gattgaaaat ccatgatagc ccgttttttt tattgaaaag attacttatc
    16621 tactctgact aaggtaagcg ccccatagaa cagtacctta ctaacaaatg cacctcaaga
    16681 tatacttgag ctatcaagaa gtctggaggt tttctatgta taa
//
LOCUS       DAXWLB010000052        16484 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXWLB010000052 DAXWLB010000000
VERSION     DAXWLB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.052"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(96..371)
                     /locus_tag="ACGE4H_001318"
     CDS             complement(96..371)
                     /locus_tag="ACGE4H_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFF8364746.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(614..1924)
                     /gene="der"
                     /locus_tag="ACGE4H_001319"
     CDS             complement(614..1924)
                     /gene="der"
                     /locus_tag="ACGE4H_001319"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFF8364747.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2106..3335)
                     /gene="rpsA"
                     /locus_tag="ACGE4H_001320"
     CDS             complement(2106..3335)
                     /gene="rpsA"
                     /locus_tag="ACGE4H_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFF8364748.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLGVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3470..4150)
                     /gene="cmk"
                     /locus_tag="ACGE4H_001321"
     CDS             complement(3470..4150)
                     /gene="cmk"
                     /locus_tag="ACGE4H_001321"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFF8364749.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4199..4834)
                     /locus_tag="ACGE4H_001322"
     CDS             complement(4199..4834)
                     /locus_tag="ACGE4H_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFF8364750.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4900..6303)
                     /locus_tag="ACGE4H_001323"
     CDS             complement(4900..6303)
                     /locus_tag="ACGE4H_001323"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFF8364751.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6290..7321)
                     /locus_tag="ACGE4H_001324"
     CDS             complement(6290..7321)
                     /locus_tag="ACGE4H_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8364752.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7352..7573
                     /locus_tag="ACGE4H_001325"
     CDS             7352..7573
                     /locus_tag="ACGE4H_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFF8364753.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7738..8475)
                     /locus_tag="ACGE4H_001326"
     CDS             complement(7738..8475)
                     /locus_tag="ACGE4H_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFF8364754.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8628..9968)
                     /locus_tag="ACGE4H_001327"
     CDS             complement(8628..9968)
                     /locus_tag="ACGE4H_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8364755.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10205..11728)
                     /locus_tag="ACGE4H_001328"
     CDS             complement(10205..11728)
                     /locus_tag="ACGE4H_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8364756.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12106..12717)
                     /locus_tag="ACGE4H_001329"
     CDS             complement(12106..12717)
                     /locus_tag="ACGE4H_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8364757.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12840..12965)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13047..13763)
                     /locus_tag="ACGE4H_001330"
     CDS             complement(13047..13763)
                     /locus_tag="ACGE4H_001330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFF8364758.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13769..14338)
                     /gene="scpB"
                     /locus_tag="ACGE4H_001331"
     CDS             complement(13769..14338)
                     /gene="scpB"
                     /locus_tag="ACGE4H_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFF8364759.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14322..15092)
                     /locus_tag="ACGE4H_001332"
     CDS             complement(14322..15092)
                     /locus_tag="ACGE4H_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFF8364760.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15089..15460)
                     /locus_tag="ACGE4H_001333"
     CDS             complement(15089..15460)
                     /locus_tag="ACGE4H_001333"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364761.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15479..16366)
                     /gene="xerD"
                     /locus_tag="ACGE4H_001334"
     CDS             complement(15479..16366)
                     /gene="xerD"
                     /locus_tag="ACGE4H_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFF8364762.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4625 a         3573 c         2791 g         5495 t
ORIGIN      
        1 ttcggaatct ctgtctctta tttattcctt ttctgctagg aaataaaaag aaaagcactt
       61 gtagccctag agccacaagc gctttttcaa ctgtcttatt taacagcgtc ttttaatgct
      121 ttacctggtt tgaatgcagg tactttgcta gctggaattt cgatttcttc accagtttgt
      181 gggttacgac ctttgcgagc tgcgcgttcg cgaacttcaa agttaccaaa accgattaat
      241 tgaacttttt cgccgttagc taaagcatct tgaatagttg aaaatacagc atctactgct
      301 gcagttgcat cttttttagt taaatcagta gctgaagcaa ctttttcgat taattctgct
      361 ttgtttgcca tggatagatt cacctcctcc acaagagata tgatagaaat atcatttagt
      421 taaaactttc tgagtggtcc agaagtttaa aaagaaatat tgattaactt caatattctg
      481 ttatcacgat atcatatgaa ccgcttaata gcaaggtttt taccaatttt cacatgcttt
      541 ttttagtcat agaaacgtaa caaacgaaac tgtctttttc ttttgaaaaa aaaacgtcga
      601 tatggtaaaa tgcctatttt ctcctacgag ggataattcg gatggacgtc ccttcaaaag
      661 taaatgcttt tctgatttga ttttctaaga aacgttcata agaaaaatgc atcaattcct
      721 cttcattgac aaaaacgaca aatgtaggtg gtttgacagc gacttgtgta gcataaaaaa
      781 tcttcaggcg ttttccttta tctgttggtg ttggattgat tgctacagca tccataatga
      841 tatcattcaa cacagccgaa gagatacgaa gattctggtt catacttact tgttcgatca
      901 tttcaggcag acgttccaac cgttgtttag tcactgcaga aacaaaaacg attggagcgt
      961 aatcaagata ccggaattca tcacgaatct cttgttcgaa atcacgcatt gtattggttt
     1021 ctttctttac aagatcccat ttattcacga cgataataat tccacgacca gcttcatggg
     1081 cgtaacccgc tactcgcttg tcttgttccc gaatcccttc ttcagcattc agtaccatca
     1141 aaacgatatc agaacggtca atggcacgca ttgcacgcat cacgctgtat ttttcagtat
     1201 tttcgtatac ttttccgcgt ttacgcatac cagctgtatc tatcattaag aatttctgac
     1261 cactttcaga aacgaagtgg gtgtcgattg cgtctcgggt cgttccttca atatccgaaa
     1321 caattacacg ttcttctcct aaaatcgcat tgatcaaaga ggatttacca acatttgggc
     1381 gaccaatcaa actaaactta atcgtgtcgt cgtcctcttc ttcagtctct tcagagaaat
     1441 acttcactgc ttcatctaaa atatcaccaa tccctagtcc atggcttcct gaaacaggat
     1501 aaggatcgcc tagccctaag gaataaaatt catagatatc gtttctcatt tctggattat
     1561 ctactttatt gactgctaaa atcactggtt tattacttct gtataaaatt ttcgcaacca
     1621 tctcatctgc gtctgtcacg ccttcgcgtc cgctgactac acaaatgatt acatctgctt
     1681 cttcaatcgc gatttctgct tgatgcttga tctgatccat aaaaggttca tcgccaagat
     1741 caattccgcc tgtgtcgatc acgctgaatt ctcgacctaa ccattcacct tttgcataaa
     1801 tacgatcacg agttactcca ggagtatctt cgacaatcga gatacgctca ccagcgatgc
     1861 ggttaaaaat cgtagactta ccgacgttcg ggcggccaac gattgcaatt gttggatttg
     1921 ccatattatt tcctcctcac aagtttccaa tctcacttag tttaacgaat gcattgccaa
     1981 ctttcaagca tagattgtca attaaacgaa gtatttttat tccttattga aatactaatc
     2041 gcttttcaaa agaaaaactg agacaaaagc agctgctttc atctcagtca cagatacaaa
     2101 atcaattatt tttcagaatc atcggttgct ttttcttcta cttcttcttt caacgcatca
     2161 cctaaaatgt ctcccatagt aaatccagta ttttcttctg gtaattcata agactcaaca
     2221 ttttttggtt cttcttctga agctggtttt tcttctaaag ctttgatact caatgcaata
     2281 cgatgttctt ctggatggat ctctaatact ttcactttca cttcgtcacc ttcatgaagc
     2341 acttcatgtg gtgtcgcaat atgtttatgt gaaatttgag agatatggac tagcccttct
     2401 actccaggga acacttcaac aaaggcaccg aagcttgtca aacgtttaac tgttccgtct
     2461 aatactgtgc caactggagc ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct
     2521 ttgattgaca aggaaacacg ttcttgttca ggattgatag acaacacttt gacatttaca
     2581 tcatcattga cagccaatac atcacttggt tttgctacat gactatgtga aatctctgag
     2641 acatggacta gaccgtcaat tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt
     2701 gcaacacgtc cactgagaac atctccctca tggatagttg aaagaatttc ttttttcttt
     2761 tcttctcttt cgttttccac taccgcttta tgcgataaga tcaaacgatt ttctgatggt
     2821 tcgatttcga tgattttaaa gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca
     2881 aaatgatctt ccaccattga tgcagggaca aaaccacgta caccgacgtc aacgactaaa
     2941 ccgcctttga caacatttgt aacaggtgct tcgatgacct tgccctcttg gaaatctctt
     3001 tcgatatctt cccaaacttt tttagcatct aaacggcgtt ttgaaagtag gtagctgccg
     3061 ttttctttat ctttgccgat cgtactgata acgcctaaat ctaatacatc accgactttt
     3121 actacttcgc tgatatcttc taccggcaaa gttgataatt cttttgccgg aacgactcct
     3181 tcaactccag ccccttcgat tccgacaatc acttgtttgt cttcaatgac taaaacttcg
     3241 cccttaacaa catcgccaac ttttacttct tgtacgcttt tcattgcgtc ttccattgac
     3301 tggttgttct cttgattttg atcaaattct gtcataacac ttgtcctcct aaccaacatt
     3361 ctgccgtaag tacggtactg tttttagttt aattgattat tcaaaaaaaa taaagcgatt
     3421 tcgttcgttt tccttgaaaa aacaattgtt tttcattttt tttcttaatt taaaaaagaa
     3481 gataaccttt tttcaaaaca acagctttta ttgctgcaac gacttctgga atcgatttgc
     3541 cagtcgtatc gatacgaaca gcatcctctg cttgcttcag cggagagacg tcccttgttg
     3601 aatcataatg gtctctttcc tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc
     3661 ctttttcttg gttctctttg aaacgtcttt ctgcacgctc acttacgctt gctactaaaa
     3721 agattttcac ttctgcattc ggtaatacag ctgtcccgat gtctcttccg tccatgacaa
     3781 cgccaccgcg ttcccctatt tttcgttgga cagcaactaa ttcttctctg acttttccat
     3841 gtgcagagac ttcagaaaca gcttttgtga catctggttg acggatttct aaagtcacat
     3901 cttttgatgc tatgaataca tgctgtccat cttcttgttg cgaaaaagag ataggatact
     3961 ttttgatcag cgaaactagc ccttcttcat ctgaaaaaga tacattgttc gtaatagcta
     4021 aataagttac tgcgcgatac atggcaccgg tatctgtata aatataattc aattcttttg
     4081 ctaatatctt ggcaacggta cttttcccag atgaagcagg cccgtcgatt gcaatactga
     4141 tttgtgtcat gatataactc ctttttctca gaaaaaaagc cgcaaagcgc ggctattatt
     4201 atttaattct taattcttga ccagggtaaa gcataaagtt gtttggatct aagccattca
     4261 attcaaacaa ttgatcagtg gtgatccctg cacgctcagc cacttgtttt ggaccttcgc
     4321 ctgcttgaac agtcgttgtt gaaccggctg cagctgatga agaaggtgtt gtttctgctg
     4381 cattgctgct tgaatcccct gcttgataag ttgcctcact tgaggaagtc ggtgttgtct
     4441 ctgctgcaat actgctttct gacgttacac ctgtatcttg agacacagta gattcttccg
     4501 cgattgtact agattctact gttgaagtct cagttgactc ctcttttgtt gtacttgtag
     4561 aactttctac tactgatgtc gaagtttcag aagaagttgc tgctgttgca ttgttgcttg
     4621 atccatttct gatccagaaa taagcacctg ctggaatagc gatacataag accagtaaaa
     4681 tcaaaaatag cgaaagaaat aatgtatttc ctttcttttg ttgtctctga gaacttcttg
     4741 atgaaacttc atcatctgta tcgtagattg gttgttccca tggttcttgt gtttcgttat
     4801 tttctgtaga ttgatgtcta tcttttctgc tcactgtttt acctcctaaa ctctatcgtg
     4861 tgtattatac cataggaaaa agtgtcttgt cctaaaaaat caatgttcca tgtttttttt
     4921 aaataaattt agtaaaatcg tttcccaatg aagttgttca gtcttagaaa aaagcttcct
     4981 ctttggcaga agttctgctt ttgctccatc aatatcacaa caattctgcg gcttttcgga
     5041 gagtttttcg ccaaaataag ctaagatgaa ttttctgcgg cagttttctt cattaatata
     5101 gcgaagcatc tgctgcaggc ggaattcttt ttctttttcc tgacgtttga gccgttccac
     5161 ccaattttct ggtttttcag actgctggat cagctccatc catttttttt gcagctcatc
     5221 aaagggagct tgctttgtgg catattccaa ataaagctca aaactttgtc gctgttctct
     5281 agaaagttga ttaaagaaat aatggatacg ttcatcccca ctttgataaa aaagaattgc
     5341 tgcactttct tcctgatctc tgcctgctcg accgatttct tgtacatagt tttctagact
     5401 gtctggcaaa tcataatgga ccacataacg gatatcagat ttatcgatcc ccataccaaa
     5461 tgcattcgta gcaatgagaa actgcagctg attttttaca aactgctgtt gaagttgtct
     5521 tctttgagct gcatcaagac caccgtgata gtatccaact gtaaaacgtt ccctgaataa
     5581 atgatacaat cgttcgactt ctttttttgt tgcacaataa atgattgccg ctccttgtgc
     5641 tctttccatg aagtgttcaa gttcttgttc tttttgctgc gttttccgaa caaaaagcga
     5701 gatattttgc cgattgactg attgtctgat ctcttttggt ggtcgatcaa atagtacatg
     5761 acgtatatct cttgctacta aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt
     5821 tcctaaaagc tttcggattt ttcctaactg ttgatattct ggacggaaat caactcccca
     5881 ttgagacacg caatgagcct catctactac atataacgca atattttgtt ttttcagatg
     5941 ctctaaaaga gaaggattag tcaacatttc gggactcaaa aacaaaaatt tatattgcga
     6001 tagatgacgg aggacgtatc ttctttcaga ttgcgaaagt aaactattga aagcagctac
     6061 tcgtttctcc cctcgttttt gtaattgcgt cacttgatct tccattagag aaagcagcgg
     6121 tgacacgatg atgaccagac cttccattaa ataccccgtc aattgatagc aaaggctttt
     6181 tcccgttcct gtaggcaaaa tagcgagagt atcttctcca tcaagcaacg cctgaataat
     6241 ttcttcttgg ccgggacgaa atgaatcaaa tccaaaatat ttttttaatt catttattaa
     6301 cattctgtat cgcttccttt ctcaatagat agatttgata caaacgaaat tctccataat
     6361 ctaattgtcc atctacattc cattcgctaa agcgccactc tcgcacggaa tccttatttg
     6421 gttccaaacg ttcgattgtt tctgcagaga gaatccactc gtatggaaaa tcatcaaaaa
     6481 acaaggccca ctcaataaga tgatctgtta ctgttcctct ttttaaatga cggattgcca
     6541 tgatctggtc aattgtctga ccattcataa acatctgctt ggtgatcatc atgctttgat
     6601 tgaaattttg ctgtaataat gaatcgatca aagcgtgcca aagcgaatct ggccgttttt
     6661 cgatttgtgc taacaaaagg tggatacatc tgctttgttg cagataaacc tctgactcat
     6721 cattgttttt tgccagctga taaggaagca gccctgttcg ctcatttcca gataattggt
     6781 tagctaaaaa atctgctgct tcttctggta tctccgaaaa cagctgcacc aaactctcat
     6841 acgcagtatc aatcaaaatt tcacgtggca attggctttc actaagccat ttttttagtt
     6901 ggaaagtata aaaaggactt gtctctgctg gaagatagtc ttgaataccc gttgccagat
     6961 tagaaattac ttgaacagca aatttaatga gccgccaact agtttcccct gttctgccat
     7021 agcggtcaaa acgcaaacca gtatgttcta tttgatgttc ggacaacata tcagcccctg
     7081 caggcgttag ctttgcttca ttttcattta tttcgatcca accttgctgc gcaatttttt
     7141 gcaaaatttg ataaaactta tcctgcttca attctggaaa actgttatgg ataaacaaaa
     7201 gttcgtgggt aaatccataa ataagtactg aagtcgttcg ttttcccaca aggagttggt
     7261 aaagagaact aatccgcagc ttgtgtcctg atgaaaatag cgataaaata aaaacaatca
     7321 tattattctc cttaagttag gatggttaga aatgtcacta ctatgtaaac ttgtacctga
     7381 acgctgtatt gcatgcggtc tttgtcaaat cgaagcgcca gatattttcg attatgatga
     7441 agaaggaatc gttttatttg cccaagaacc agaggctcat caacagttcg tgatgccgga
     7501 agaagaaaca tatgtaaaaa aagcgtatca aaaatgtcca gttcgagcga ttttattaga
     7561 aaataaaata taaatataaa cgaaacttta aaaacgtaca ctaagttctc taaacttgaa
     7621 aataagccgg gatctgtttc tcttgttccg tgaaatcccg acttattttg aaagattcat
     7681 ttgctatcta tagttaattg actagttttt tcacggaccc tataggaccc atttatatta
     7741 tttcaacttt ttcagccagt tttccaatgt attgacgatc acagtttgct ggtctgcgtt
     7801 agaaatttct gcctctccat ctcctttttg ctcaccatag ctgccaaaac ctccatgatt
     7861 tccgccattg attgatacat actctgttct ttccggtaaa tactgcttag cttcttgata
     7921 agcttcctga ttcaatactt tatcttttgt cgccgtgatc gataaaacag gaacagctga
     7981 ttctgtcaat gttccttttt tgtcgggata acttgccatg aagaataccc cttttaaatg
     8041 tttgtctgaa tgattcttat tgacataacg acttgccatt acgccaccta atgaatggcc
     8101 accgatcaca taatcggaat ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc
     8161 catcactgct agatcaagtg gcattttgac aatagcgact gaaaagccgt tttttgctaa
     8221 atcttgcgcc caaatactat aactttctgg ctctacgaga gcaccaggat aaaaaataag
     8281 taatggagac tttccatgat tctttttctc tccttcaaaa aataagtaat cttctttgtt
     8341 ttttccttct tttaccaaca cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat
     8401 atagtaataa ccggctccac cgagtaatcc taaaatgatg actaagccaa ttagtattcg
     8461 tctaaatatt ttcactttct cccacctttt ctatctgagt gacccctttt tcttaatcaa
     8521 gtatgattat accttaaaaa aataagagag aaatgcaaac tttccagcat caaataaaaa
     8581 aagacggaat atccatttga atatccgccc tttttgtatg tttattttta tttagcacat
     8641 aaacgataca cagcttctgc ataaatttcc attgctttat acatactact taattcccat
     8701 cgttcattcg cctggtgcat aaagtcagga gtatcaggga acatcgcacc gaatgccaca
     8761 cactggttca tcgtacgagc aaaagtagcg ccgcctgata caaacggttc agtcatgtct
     8821 ccagtaatat cgcgatatgt ccccaataga tttttcacta actcactgtc tagtggcacg
     8881 tataatgaat caaggaagtc aaattcttca taagtcaacc catatttttc tgctgtttta
     8941 gttaatttag ctactaaatc atctttcttg aatgtaacag ggattctcat atctacaccg
     9001 atttttgttt gttctggtgt gatttccaaa cttgcaaagt tcatcgttag ctcgccggat
     9061 tgttcatctt ctgttttgcc cacgacagct tcaccagttg cattttcttg aactaatttt
     9121 cctaagaaat tgattgggcc aaaatcaaat acttctgaaa gagcaattgc cagacgagaa
     9181 atagcattca ctccttgtgc tgcatctttc gcgtggatac ttttaccaag aacgacgatg
     9241 ccttcacctt gttcttcgaa atcgaattcg tgttttttca atgcttcttt tacttcagaa
     9301 agcttttctc cagagtaagg agctgcatca ggaacaacat tcaacgctcc tccagctttt
     9361 acagaaaact cgttcgttcc tggtcctgtc aagtaagctt gcaataagcc tttttctgca
     9421 taaattaaag gaaattcagc atctggcgca aatccttgcg taattccttc ttctttttcg
     9481 ttatattttt ctaaacaacg ccataaattt tcttcatctg taccaaagat aaaacgaata
     9541 cgtgtcttga attccacgcc agcatcaatc aaagctttga cggcatacat tgccgcaata
     9601 gaaggacctt tgtcatcttg ggatccgcga ccaactaacc agccgtcttt tatagttgga
     9661 tcgaatggtt ttgtttccca attattttcg tcccctgctg gaacaacgtc catatgacac
     9721 aagataccga ataattcatc tccagagcca atctctgaat aaccgtaata gccttctgga
     9781 tctttaaatg ttctaaatcc tagcttttca ctgatttcca atactttatc caaagcttca
     9841 atcacttttt taccgaaggg atgtccttgt cctgaatctg cttcatctag tacggaagga
     9901 attcggatca attcgctcaa tgattctaat gcttgttcat gatgttgttc tgtcacaaat
     9961 tttttcatga tattatcctc ctaatatgtt aaaaagggtc atgtcaaatt cctccgacac
    10021 aacctcttct tctcaaataa tgacgtttta agcctgctcc atcagcaagc agaatgttct
    10081 taacaaatga tgaaagaaaa aacactcact gaatggagct attgacttgt ccacgtgctt
    10141 atcgatagcc gtttatcggt ttcttcattc ttatgataga ccttgtacct gtatttgtct
    10201 aggtttagaa cattgctgct acacctagga tcacacagat ggctacaaat aatatcgcaa
    10261 ttaatttacc ggtaaatttc caccacactg aaacatcgaa acgagcaatt gctacagcac
    10321 ccattacgat agcagatgtt ggtgtaatta agtttactaa accagatgca gattgataag
    10381 cagtgattac taaatcctta ccgacgccgg caaattcacc cataggtccc atgattccca
    10441 ttgttgcagc agctaagccg gaagttgaag gaatcaagaa tgacataggg atatagaaaa
    10501 taaatgtaat aatgatgaat acacttgaag ataatgaact taatccttgt tcgccccaat
    10561 gtaagatggt gtctgtgatt aatccattgt tcataaccac ttggatacca cgagcaacag
    10621 ctactacgat tgctacaccg atcaaatcag aagcaccagc taaaaattca gagacaaatt
    10681 cagactcttt catgccataa gtgattgcta ctacgatgga cattactaag aataacatag
    10741 taatttctgg gaagtaccag tcacctaatg gaagcatgct tttacctaga accgtaccta
    10801 gtaccggaag gtttgttaac cagtttgtca atgattcaaa aatcgtgaaa ttcgcattca
    10861 aggttgtcca aggaatcaaa ctgatgatca tgataccaaa agtaataaag aacaatacat
    10921 taacatgttt ttgttttttc gtgattactt cttgacgacc gattgcttca atatcgaaat
    10981 gcttcatatc ttctttacgt tggttgtata ctaatgattt tgttggatct ttttcgatct
    11041 ttgatgcata acgatacaca tatacaatag aaatagctaa caaaataacg aacatcaaga
    11101 atcgaagacc aataccttca cccatcgaaa tcccaacagc ttgtgaagct acgcctgttg
    11161 caaaaggatt taccgttgaa gctagacagc ctacttgaga ccctactaat acgattgcta
    11221 cagctactac tgtatcaaag cctacagcca tcatgactgg gatcagtaaa gcatagaatg
    11281 gaatcgtttc ttctgccatt ccgtaagtag agccgcctaa tgcaaataac agcatcaata
    11341 ctggaataag cattttttct tttcctttga atttcttcac aactgaagca atcccggcat
    11401 ctagtgcccc tgttttgttg ataataccta aaaatccacc aacaaccaaa atgaagaaag
    11461 agattgggat tgcagcaggt gtttctttag ttagctcccc cgctggtaca cctagcatcc
    11521 cattgattgg cgccatgaaa atatcccaca gcccttgagg attttgttgt actcgttcat
    11581 atgatccagc gacgatgttg cctgcatcgt ctacactata gtgcccagct ggtataaacc
    11641 aagtaagtac agcgattact gcaataataa taaataagac cgtatacgct gaaggcattt
    11701 gaaatttttt cttttttgtt tgttccatct tcttcttcct ttcttttccg ttagagacaa
    11761 caattttggc tctaatgtta ttttatcaaa aagagtatca gaacaaaaat aataacacgc
    11821 attttgtaaa acttttgttc aaaaagttcg tttttcccta ttccctcctc ttttttcagt
    11881 tttttaaagc tttcttcttt ttcattgtag cgtttccttt tttttataaa caaacatttt
    11941 cgtcttttaa aaggagaaga atttcacatt tgcaaagaat tgattgattt ttatgcataa
    12001 atcaaaaaaa tactcaaaac aaacaaataa aacgtttaca ttattgcatg tcttgtattt
    12061 ttttccaaaa aaaaagacca agaaatttct tgatcttttt gatatttatt tgactgctgt
    12121 atgacgttgt tcgaactgat gttgttttcg acttaaccat ggaagaagtt gcgcatgcag
    12181 cactaaaaag acagcactca cgatgaatcc tttgatcaag ttgaaaggaa cgatcccaat
    12241 cagtacatag tttgctaaag acatgcccag catctgtgtt gcgttcagcc ctaaaaacat
    12301 taagtaaaga ggcgtaataa cgaaataatt tgcgatactc ataaaaatag ttagagcgat
    12361 ggttcctgta atgacaccta aaaatttgtt tcttccttta tgctgtttct ttacactaaa
    12421 gaaataataa atcgggaatg taaagatcgt tgttgataag aaactagcag tgtctcctac
    12481 caaattatcc ggagaaaaac ctgtagtcag cagatgtaat actgaacgca agaacgctgt
    12541 tgcaactccg gccgctggac cgaataagaa catgctgatc aataccgaga tatcactaaa
    12601 atcgatcttc aaaaaactaa aagccggcat gatcggaaaa gcaaaatact gtaaaactaa
    12661 tgcgatcgca gcaaacatcg ctactgcaac cattttctgt acacgggtgt tcttcatttg
    12721 aaatcctcct gttaggactc ctcttcagcg aactttttga cgtttctctc caaatgaaaa
    12781 aactctattt tccagggaaa atagagttag agttatttga tcaagcgtcg tcaaataagc
    12841 tctatcttct ttatccagac tatactgtcg gtatcggaat ttcaccgatt cagctatttt
    12901 tttgcaaaat aggtcgtgga ctataaccac cggtcgggaa tttcaccctg cccctgaaga
    12961 cgaaccttat ataatttttg tttacatatt cattatacat atctattcaa aaaacgcaac
    13021 atctaacaat tataaaaagt aagtgttcat tttgagtggt tcagcaaact gctgacttct
    13081 tttttgttca gttcacggta atccccagga cgtaggcctt gtaaagtcag atctccgtat
    13141 ttttcacgtt ttagtttttg cacaggaaga ccaactgctt gaagcatatt tttcacttga
    13201 tgattacgtc cttcgtggat cgtcaattcc acgatactgt ggtttgtctt aggatccact
    13261 gataaaattt ggaatttagc tggagatgtt tttcgtcctt cgatctttat tccttttgtc
    13321 aaaggtgcaa gcatcgattt tgtagcgatc ccttttacct ttgcaacgta aactttatcc
    13381 acttcatgtt ttggatgtgt caggcgctga gcaaaatctc catcatttgt cagtagcaag
    13441 atccccgaag tatcgtaatc cagtcgacct acaggataga tacgttcttt taccatcggc
    13501 aaaagatccg taacaacttt tcttcctttg tcatctgcaa cagcagaaat aactccttta
    13561 ggtttgtaca ataaatagta accgtattct tcttgataaa ttgggacttc atccacttcc
    13621 acagtgtcat gtttcccaac ttttatccct aattccctta caacttgtcc gtttactttt
    13681 acacgtcctg caaggatgaa ttcttctgct tttcgacgag aagtgatgcc agcgtgggca
    13741 attactttct gcaatctctc cattacaatt attctccttt attcattcca tcaaagaata
    13801 gatctggtga cacgtcttct tcaagtgaaa tttccatttc ttgtgtatca ggcagttctt
    13861 ctaatgattt caaaccaaaa taatctaaga agtagccagt tgttccatat aatatcggcc
    13921 gacctggtcc ttctactcta cctttttctt caatcagttt atgggcagct agacgctgta
    13981 ccgctcctga aacctgaact cctcgaatct gctcgatttc agctcttgtg attggctgtt
    14041 tataagcgat gattgctaag gtttcgacgg cggcttgaga taatgtgttt gatgtccctt
    14101 gcgcatatcg cttaagtaag ggagccagct cttttttagt cgttaataca taatggcttt
    14161 ccgtttcaaa aatatgcaaa gctgagtgcg agttttgagt gtaagattct tttaattctt
    14221 tcactaaact tgtgacttct gcttcgctga gatttaacag tccgcttaat tcttcattac
    14281 tgatcccttc ctcgccaaca ataaaaagaa gggcttcaag ttcacttaat ttcgtcatgt
    14341 tcggtatctc ctactgctgg ataaagcata attgattcat agttttcttt ttgttgcgca
    14401 atgatctgcc cttttttcat caattctagc aaagccataa aagtagtgac aacttcactt
    14461 ttagaaggtg actcaaaaaa agaatcgaaa gtacagccat gcgcatttct ttttatacgc
    14521 tcttcgataa ataccattcg ttcttcgatc gttgtgtcat cgggcgtgat cgttttttcc
    14581 actttttgag aaaatttctt acgttctaag attttctgca tggcacggat caaatcgatt
    14641 tttttgactt gtcctcgctt caacggcttt tcagcatccg ctaacccatc gatgttcata
    14701 ggttcttttg tgtaataaag acttcgttcc tctgcttttt ctgtcagtct ttcagctgca
    14761 tatttatatt tacggtattc taaaagctga gtgaccaatt cttctcgagg atcttcttcc
    14821 cataattctt catcgtctgt gccgaattct tggacaggaa gcaatgtctt gcttttgatt
    14881 gccatcaatg tagcagccat cactaagtat tctcctgcaa cagctagctc gagtgttttc
    14941 attgtatgga tataattcat atattgctcc gtgatcgtag cgatcggtat atcatatata
    15001 tcgatttcca tttgctgaat cagatgaagc agcagatcga gaggaccctc gaatacatct
    15061 aatttaatat tgatctccgt caactcattc actctttctt ctttttcttt gctagccatt
    15121 ttccaatgta aggctctgtg atttcagttg cacggatgga gacatccggg tacttttcct
    15181 gtaattcatc taacatttga aagcccatat tctcaccgcg ataagacgga ttcaaagaaa
    15241 tgtcatggat gatcagctga tcgttcgttg cccagtaaat accaataatc ccttgaatgt
    15301 tggttgaacc ttctggcgta aagaaaaaaa gttcataatc gtcttcagat tcgtacgtat
    15361 caatctcctt caacaaagaa gttttacctg tcagtttctt atggaaactc aatagtccca
    15421 tagcaatttt acggttttct ttccggtaat gtgttaacat cttctcacct tcctatgact
    15481 aagcccttgg aaaatgctgc ttataaacat ccgtcattct tttcttcgtg atatgtgtat
    15541 aaatctgagt cgtagagata tccgcgtgac ctaacaattc ctgtactgtc cgcaaatcag
    15601 ctccattttc tagcagatgt gtggcaaagc tatgccgcag cgtatgtggt gttacttctt
    15661 tgtaaatccc agcctctcgt actaactgct tcagattttt ccagattcct tgccgagaaa
    15721 gacctttccc atgattattg acaaatacat gcatttcaga tgcgtccttt acaagtaatg
    15781 gacgtgcttc gtctaagtag cgttccagcc attggatcgc ataatcacct aaaggaatga
    15841 tccgctcttt atctcctttt ccaactgtct gtaccagtcc aagagaaaga tgaagatctc
    15901 ccaatttcaa tccaatcaat tcactgacgc gcatcccagt agcatacatg acttccaaga
    15961 ttgcacgatc tcttatccct aacgtttttg tcgtatcagg tgcttcaatc agcttttcta
    16021 cttctgtcag agtcaatgta cttggtaatt tctgtgcttt cttcggcgta tcgatatgtt
    16081 gcatcggatc atgatcagtg atacgctctt gccgtaaaaa ctgatggaat cttctgagac
    16141 ttgagatcat ccgagtaaca gttgctgtcg cttttttttc ttcatgcaac ttctccagaa
    16201 aacggatgac taaaaagcga tcgacatcct gccatgaagc aattttctgc tcctgtagaa
    16261 aatgcaagta ctgttccaaa tcacgttcat agctttgtcg tgtattggcg gacaatcccc
    16321 gttcaatatt caagtagtgg agataatcgt tgatttgctc tttcataata atccccctca
    16381 tgcctctcta tcatatcaga taccgcttca ttctgatagt ctttttccat aatctgagca
    16441 aagaaaaagc cgtctagaca ctcagacgga ctggtttctt tttt
//
LOCUS       DAXWLB010000053        15980 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXWLB010000053 DAXWLB010000000
VERSION     DAXWLB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.053"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            226..894
                     /locus_tag="ACGE4H_001335"
     CDS             226..894
                     /locus_tag="ACGE4H_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFF8364763.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACGE4H_001336"
     CDS             complement(1094..1480)
                     /locus_tag="ACGE4H_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364764.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACGE4H_001337"
     CDS             complement(1452..2834)
                     /locus_tag="ACGE4H_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8364765.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACGE4H_001338"
     CDS             complement(2990..4177)
                     /locus_tag="ACGE4H_001338"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFF8364766.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACGE4H_001339"
     CDS             complement(4184..5215)
                     /locus_tag="ACGE4H_001339"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFF8364767.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACGE4H_001340"
     CDS             complement(5231..6685)
                     /locus_tag="ACGE4H_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFF8364768.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACGE4H_001341"
     CDS             6860..7765
                     /locus_tag="ACGE4H_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFF8364769.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACGE4H_001342"
     CDS             7856..8533
                     /locus_tag="ACGE4H_001342"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8364770.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACGE4H_001343"
     CDS             8530..8700
                     /locus_tag="ACGE4H_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364771.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACGE4H_001344"
     CDS             8814..9710
                     /locus_tag="ACGE4H_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8364772.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACGE4H_001345"
     CDS             complement(9765..11135)
                     /locus_tag="ACGE4H_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8364773.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACGE4H_001346"
     CDS             11373..12119
                     /locus_tag="ACGE4H_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8364774.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACGE4H_001347"
     CDS             12116..12955
                     /locus_tag="ACGE4H_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFF8364775.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACGE4H_001348"
     CDS             12956..13906
                     /locus_tag="ACGE4H_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFF8364776.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACGE4H_001349"
     CDS             14047..14799
                     /locus_tag="ACGE4H_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364777.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="ACGE4H_001350"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="ACGE4H_001350"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFF8364778.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5187 a         2995 c         3219 g         4579 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaagcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta
//
LOCUS       DAXWLB010000054        15974 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXWLB010000054 DAXWLB010000000
VERSION     DAXWLB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.054"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            39..269
                     /locus_tag="ACGE4H_001351"
     CDS             39..269
                     /locus_tag="ACGE4H_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFF8364779.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            366..1667
                     /gene="murA"
                     /locus_tag="ACGE4H_001352"
     CDS             366..1667
                     /gene="murA"
                     /locus_tag="ACGE4H_001352"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8364780.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1680..1856
                     /locus_tag="ACGE4H_001353"
     CDS             1680..1856
                     /locus_tag="ACGE4H_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8364781.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1991..2287
                     /gene="yidD"
                     /locus_tag="ACGE4H_001354"
     CDS             1991..2287
                     /gene="yidD"
                     /locus_tag="ACGE4H_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFF8364782.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2472..2939
                     /locus_tag="ACGE4H_001355"
     CDS             2472..2939
                     /locus_tag="ACGE4H_001355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364783.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3024..3908
                     /locus_tag="ACGE4H_001356"
     CDS             3024..3908
                     /locus_tag="ACGE4H_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8364784.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4022..4258
                     /locus_tag="ACGE4H_001357"
     CDS             4022..4258
                     /locus_tag="ACGE4H_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFF8364785.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4346..4426
                     /locus_tag="ACGE4H_001358"
     tRNA            4346..4426
                     /locus_tag="ACGE4H_001358"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4380..4382,aa:Tyr,seq:gta)
     gene            4433..4504
                     /locus_tag="ACGE4H_001359"
     tRNA            4433..4504
                     /locus_tag="ACGE4H_001359"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4465..4467,aa:Gln,seq:ttg)
     gene            4629..4799
                     /locus_tag="ACGE4H_001360"
     CDS             4629..4799
                     /locus_tag="ACGE4H_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364786.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5019..5726
                     /locus_tag="ACGE4H_001361"
     CDS             5019..5726
                     /locus_tag="ACGE4H_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8364787.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5785..6429)
                     /locus_tag="ACGE4H_001362"
     CDS             complement(5785..6429)
                     /locus_tag="ACGE4H_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFF8364788.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6555..6842
                     /locus_tag="ACGE4H_001363"
     CDS             6555..6842
                     /locus_tag="ACGE4H_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364789.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6950..7270
                     /locus_tag="ACGE4H_001364"
     CDS             6950..7270
                     /locus_tag="ACGE4H_001364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8364790.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7351..8664
                     /locus_tag="ACGE4H_001365"
     CDS             7351..8664
                     /locus_tag="ACGE4H_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFF8364791.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8661..9380
                     /locus_tag="ACGE4H_001366"
     CDS             8661..9380
                     /locus_tag="ACGE4H_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFF8364792.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9508..10059
                     /gene="raiA"
                     /locus_tag="ACGE4H_001367"
     CDS             9508..10059
                     /gene="raiA"
                     /locus_tag="ACGE4H_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFF8364793.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10281..12815
                     /gene="secA"
                     /locus_tag="ACGE4H_001368"
     CDS             10281..12815
                     /gene="secA"
                     /locus_tag="ACGE4H_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFF8364794.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12941..14042
                     /gene="prfB"
                     /locus_tag="ACGE4H_001369"
     CDS             join(12941..13012,13014..14042)
                     /gene="prfB"
                     /locus_tag="ACGE4H_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFF8364795.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14147..14833
                     /gene="ftsE"
                     /locus_tag="ACGE4H_001370"
     CDS             14147..14833
                     /gene="ftsE"
                     /locus_tag="ACGE4H_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFF8364796.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14826..15710
                     /gene="ftsX"
                     /locus_tag="ACGE4H_001371"
     CDS             14826..15710
                     /gene="ftsX"
                     /locus_tag="ACGE4H_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFF8364797.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
BASE COUNT         5375 a         2699 c         3480 g         4420 t
ORIGIN      
        1 ttagttatta taaaacaatc gttagaaagg acgtcattat gcaagtatac ggaattgatg
       61 cggtgcttcg aatcgtgagt catttggcgt ttatctactt agccttctgg tctttacgtt
      121 cgttacgcat tgaaactttt ttcagaccaa tgcataatac gcaaattaga atggcaatcg
      181 tacttttttc aattttttta gggtacactg caagtaattt tttcttagaa gttatcgctt
      241 tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa aggtactatt ttaaaagcaa
      301 ggatgttata atagaaaaga ttttgagtaa gtgtatgctc attggaattt tttggaggga
      361 acaacatgga agagatcatc gtaagaggtg gcaatcaatt aaatggaaca gtaagaattg
      421 aaggagctaa aaatgctgtt ttgcctattt tagctgctag tttgttagca gaagaaggta
      481 ttacgacatt agacaacgtt ccgattcttt ctgatgtctt cactatgaat caagtgattc
      541 gtcacttaaa cgtagatgta gattttgatg aacaaaagaa tcaagtaaca atcgacgctt
      601 caagacaatt agaaatcgaa gcgccttatg agtatgttag tcaaatgcgt gcttcaatcg
      661 ttgtgatggg tccgctactt gccagaaatg gacatgcaaa agtagctatg cctggtggat
      721 gtgcgattgg taaacgacca atcgatttgc atttaaaagg atttcaagct ttaggagcaa
      781 aaattattca aaaaaacggc tatatcgaag caattgcgga tgaattgatc ggtaacacga
      841 tttatttaga tttccctagt gtaggtgcta ctcaaaatat catgatggct gccgtaaaag
      901 caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc agaaatcgtt gatttagcaa
      961 acattttgaa taaaatgggc gctcaagtct atggagctgg tactgaaaca atgcgtattg
     1021 aaggcgtgga tcatttacat gcagtcaacc actcaatcgt acaagatcgt atcgaagcag
     1081 gtacctttat ggttgctgct gcaatgacac aaggcaatgt gctgattgca gatgctatct
     1141 ctgaacataa ccgcccattg atttctaaat tgatcgaaat gggtgcagaa atcattgaag
     1201 aagaaggcgg agtccgtgtc atcggaccaa aacatatctt acctacagat gtaaaaacga
     1261 tgcctcatcc tggtttccct acagatatgc aagctcaaat gacagctatc caattagttg
     1321 cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa tcgtttccaa catttagaag
     1381 agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa tgtagcaatc atggatggaa
     1441 accatgaatt gcaaggtgca gaagtatatg caacagactt gcgggcagca gcagcattag
     1501 ttttagctgg cttgaaagca aatggaatca cacgtgtgcg taacttgaat tatctagacc
     1561 gtggatatta taatttccat atcaaactac agcaattagg tgcagacgtt gaacgtgtag
     1621 acatggatca aacttctgct gaaaaaacag cacaaacgat tgcttaagga agtggctgaa
     1681 tgagtaacca acgatatatc ttattaactt tgataaagat tttaatggtg atcttattgc
     1741 ttatccttct ttttgtggcc ggaacgatga tcggatatgg tgtcatcggt ggcggcaatc
     1801 catttaaagt atttcagcca agtttgtgga ttcatattcg agactttttt cattgataaa
     1861 taagaatcac agtggttcta gtcgatatga agtgagccat gaagcagaac atgataaaag
     1921 aaaacagaag ggaacgttga cgttagtcgc gatccctttt tttagtaaaa gaaaagaagg
     1981 aatctaattg atgaagaaga tattgattta tttagttaga ggatatcagc gctttatttc
     2041 gcctgcattt ccacctagct gccgttatta cccaacctgt tccaattata tgataaaagc
     2101 aattcaaacc catggcgcgc taaaagggac ggtaatgggg actgcacgga tcttacgctg
     2161 tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg acattccgat taacgcgaaa
     2221 taagaaagat aaaaaagaac cagattataa aaaggttgtg aaaaatacgg gtagcactga
     2281 gcgataaaga aacaatttgg aaaatagctt cttatatttt gcaaaattgt gagttattgc
     2341 cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt gaaaaatacg gctagcactg
     2401 agcgataaag aaaaagagga aatttattgt caaagtaaca actccgcaac tacttatatg
     2461 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2521 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2581 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2641 aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa ggggattggc ggaaaattac
     2701 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2761 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2821 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     2881 atcaattaag ggatcaagtg attttagcaa ttcacttacc taaaaatagc tttaactaat
     2941 aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3001 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3061 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3121 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3181 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3241 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3301 ctctgtttca acttccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3361 tttatgaagg tgctgcccgc gattatcaag atgttgcttt tgttgtggca ggaactggga
     3421 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tacggtggag
     3481 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3541 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaatgcttat accggaaaag
     3601 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3661 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3721 tattaggcgg cggggtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3781 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3841 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     3901 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     3961 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4021 aatgatgtca aaaaaaaata aagatattga agtgcgtata gaagaagtga aaaaagatat
     4081 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4141 cttagcctat ggaccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4201 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4261 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4321 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4381 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4441 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4501 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4561 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4621 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4681 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4741 gggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4801 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4861 aggttggaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     4921 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     4981 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5041 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5101 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5161 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5221 catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5281 ttatggaatc acaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5341 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5401 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5461 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5521 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5581 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5641 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5701 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattccgt attagaaata
     5761 aaagagaatc agaatcacct gtttttatat atgatcagtt accagttgct cgtgataaga
     5821 aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg attctttaaa
     5881 gtggtcagca cttttttcat ctacagcaca gatgacgcta acttgatcag tatattggat
     5941 ttcttttata aaaaaatgtt ggagttcaag ataattctgt aattttccta gttgcggata
     6001 cgtgatcatc acagagatct cctgttgcaa ggtaccttct acgattccaa tgtcagacaa
     6061 tgcttgagag accgcatgag tgtatgctcg tattaatccg cctgtgccga gtttcgttcc
     6121 tccaaaatat cgagtgacta ctgcagctac attgatcagt tgattttttt tgaggacttc
     6181 caacatcggt atgcccgccg ttccacttgg ttctccatca tcgctgcttc gctggatttc
     6241 atttttttct ccaatgatga aggcgctgca gttatggttc gctttccaat gttctttttt
     6301 tatttcttga atgaaacttt ttgcttcgtc ctctgattct attcgtctaa gagaacagat
     6361 aaagcgtgat tttttgatct cgatctcact ttgtccattt tcttttattg tacgatagcg
     6421 ctcgatcatc ttgtcacctc ttttttcatt ttctttattc agtataaata aagttctagg
     6481 ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat gcgataataa agacaaaggc
     6541 aggtggatct ggggatgcag gaacgaatga aaaagtacga tgcaattacg cattatttga
     6601 agaacaatgg cggctctcaa gtaacgttga cctttaccca atttgacgaa ctgctgtttc
     6661 ccagtaacgg acttcccaaa acagcaaggg aaagtacaga ttggtgggca aatgattata
     6721 aacaccctga aaaaggtgcc tatggctgga taaatgcagg ctatgaagta gttgtcatca
     6781 atttggacaa agaatatgtc gtgttcaata aattagtgaa atctagttgg ctatttgatt
     6841 aaataaaccg cgtttagaaa acgttgacaa atttctttct ttttatataa tagagtaaca
     6901 gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga gagaaaagta tggctaaaaa
     6961 aacaatcatg cttgtatgtt cggctggtat gagcacaagt ttactagtaa caaaaatgca
     7021 aaaagctgct gaagcaaaag gaatcgactc tgatattttc gctgtgtccg cttctgacgc
     7081 agataacaat cttgcaaata aagacgtgga tgtgttgtta ttaggcccac aagttcgctt
     7141 tatgaaagct gattttgaga aacgtctgga accaaaaggc attcctttag atgtcatcaa
     7201 tatggctgat tatggtatga tgaatggcga aaaagtgtta gatcaagcaa tcacgctcat
     7261 aggtgaataa atagaacagt ataactatcg aacaagcgat ccaaaaaata gatcgcttgt
     7321 tttttcgtat atttttttag ggcgaataat atgaatcatt tttggggtag aaggcttctg
     7381 acgagagata ttaaagcaat agactgtgag gaggtaaatt taccaaatta tgagaagatc
     7441 gcagcagtgg aacgtttgag caactggatt ctttgtcatc gctgtggaat gagaacgtcc
     7501 gtcattgatg gacagcttgc agattacggt tatttttgta cacattgtct atatttaggg
     7561 cgatgcgaca ctcaacaaga tttatatttg tttgatcagc ctgaagcaaa acctcgagaa
     7621 gtcgtctttt tttggacagg acagcttact gaaaaacaaa cggaaatctc aaagagaata
     7681 ctttgccatt cagaaaaaag acatcatctt atttgggctg taacaggtgc agggaaaaca
     7741 gagatgcttt atcctatttt agtggaaact ttaaaagctg gaggaagagt agcgatttgt
     7801 acaccgcgag tcgatgtttg taatgaattg tttttacgct atcgttatgt ttttccagaa
     7861 gaaaaaatca cgttgctcca tgggaataca gcaacggctt atacctttac ttcttttgtt
     7921 atctgtacga ttcatcagct ataccgtttt tatcggtcct ttgatttaat agtgatcgat
     7981 gagatcgatg cgtttcctta taacggggat gcaggattag ctcatgctgt gcaaacagcg
     8041 attaaaccag acggacgatt catctactta agtgccacac ctgataaaaa gttactaaag
     8101 gagatcaagc agacgtttga catccacaaa cttgctttac gctttcatcg gcgtttgctt
     8161 cctgaacccg tcttggtttt catgaataat tggcgacaaa aatgcaagaa caagaagaag
     8221 ataaaatctt tggtgcggat cattcatcaa ttaattaaag aaaaccatat ccttttattt
     8281 tgtccaagta tcacgatgat gcatcgtttg aaagccacac ttcaagaggc tcttccgaga
     8341 tatgagatca cagatgtttc ttcaagagac aatcagcggg cggaaaaagt ccagaaaatg
     8401 cgagaagcaa aatatgacat actgttaacc acgatgatcc ttgaacgtgg agtcacattt
     8461 gaaagaatat ccgttatcgt tttaggagcg gatcatccgg tttttactaa atcttctttg
     8521 gtacaaatcg caggaagagc cgatcgtaaa gggccttata cgaacagtca agtatatttt
     8581 ttctatgaag aaaaaactgc agctattcga aaagcttgtc aggaaatcaa agaaatgaat
     8641 gaagaaggga agaagatact gtgaggtgca gttgctgcag taaagatatc gtccaaaatt
     8701 taacattcag cgagattatt ggtgtttcga aatacagatt acctcagact tgttcagttt
     8761 gtcgcgcaca atttcattta ttggaaacaa gaggctgttc tggctgtcaa aaaccaatcc
     8821 atttcaatct agagagtacg aaaaagaaag taaattctat ccccgtttac tgcagtgact
     8881 gtcaagaatg gaagaagaaa tatccgacct atgattttca gcaccgtgcc ttttttgtgt
     8941 acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt tatgggagat atccgactag
     9001 caggcacctt tgcatataaa tggacgcaga taaaaaaaga atacccgaca tacatctatt
     9061 gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg attcaatcaa gtaagcgaga
     9121 tgttgaaagc agcaagaatt ccttttcaga tgctactcaa aagagagaag catctgctgg
     9181 ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc tcaacctttt gaacttgcag
     9241 tcaccttttc taagatacaa aataaaaaaa tattgattat tgatgatgtc tatacaacag
     9301 gacagactct tttccacgca gcagattgtt tgcagatcgc acagccgaaa atcatccgaa
     9361 ctatttcttt ggcgagatga attagatgtg gaaatttgat agcgatttaa tttgcaaata
     9421 acctagagtt cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga
     9481 ttgtggtaga cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc
     9541 gaagtaactg aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat
     9601 ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca
     9661 gcaaaagtgg aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct
     9721 ccagatttat acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa
     9781 ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca tgaatactgc aaatgctgct
     9841 gctttattca atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg
     9901 aaacgccttt cattgaaacc aatggatagt gaagaagcag tcttgcaaat gaacatgctg
     9961 ggacataact tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc
    10021 cgtaaagatg gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa
    10081 cgaaaacttg cagcagctac tgacagctgc cgcaagtttt tttgagtttc ctcctctttt
    10141 agatgaaata tgaataaatt atgaagttct tactttaata cgcttaagat atttcaatta
    10201 tctataacta atgataaaat gaaaagtaaa gtctacatag tctggataat acacagaagg
    10261 aaaaggagat tacacaacgc atggccaatt tgattcgttc acttatcgaa aacgataaga
    10321 aagaactaaa acgtttagat agcattgcaa aaaaagttga agcatacgct gaccaaatgg
    10381 cagcattaac tgatgaacaa ttacaggcaa aaactgctga attcaagggc agataccaaa
    10441 aaggagaaac attagatcaa ttacttccgg aagcttttgc agtcgtccga gaagcagcaa
    10501 aacgtgtatt aggtttatat ccataccacg tacaattgat gggtggaatc gtgcttcacg
    10561 atggaaatat ccctgaaatg cgtactggtg aaggtaaaac cttgaccgca acgatgccag
    10621 tatatctaaa tgctctgact ggcgaagggg tacacgtagt taccgtcaat gaatatttag
    10681 caacccgtga ctcgacggaa atgggcgaac tttataattt cttaggatta acagtggggt
    10741 tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc ttataattgt gatatcactt
    10801 atagtacgaa taatgaatta ggatttgact acttgagaga caatatggtc gtttaccgcc
    10861 atcaaatggt acaacgtccg ctgaattatg ccattgtcga tgaagttgac tcaatcttga
    10921 ttgatgaagc gagaacgccg ctgatcattt cagggcaagc agaaaaatcc acagctttat
    10981 atacaagagc agacaacttc gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg
    11041 tacaatcaaa aacaatcagt ttaaccgaag cagggatcga aaaagcagaa gaaacattcg
    11101 gtttggataa cttatacgac attgaaaata ctgcattgac ccatcacttg gatcaagcat
    11161 tgagagccaa ctatatcatg cttttagata tcgattatgt tgtccaagac ggaaaagtac
    11221 tgattgttga ccagttcact ggtcggatca tggatggacg ccgttattca gatggacttc
    11281 accaagcaat cgaagctaaa gaaggcgtag agatcgaaga cgaaacgaaa accatggcga
    11341 cgatcacttt ccagaactat ttccgtatgt acaagaaact tgcgggaatg actggtacag
    11401 ctaaaacaga agaagaagaa ttcagagaaa tttataacat ccaagtatac cagattccga
    11461 caaatcgtcc ggttatccgt gatgatcgtc cagatttgct ttacccaaca ttacaaagca
    11521 agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg caaaggtcaa cctgtgttag
    11581 tcggaactgt tgcggttgaa acatctgagt tattatcaga tatgctgaat caagaacgta
    11641 ttcctcatga agtgctgaat gcgaaaaatc actttaaaga agcagagatc atcatgaatg
    11701 ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc tggtcgggga acagatatca
    11761 agttaggact tggggtaaga gaacttggtg gtctagcagt tatcgggaca gaacgacatg
    11821 aatctcgtcg tatcgataac cagcttcgag gacgtgcagg acgtcaagga gaccctggta
    11881 tgtcacagtt ctacttgtca ttggaagatg atttgatgaa acgattcggt tcagaacgaa
    11941 tcaaatcttt cttagatcgg atgaagatcg gtgaggaaga tgctgttatc caaagcaaga
    12001 tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga aggaaataac tatgacacac
    12061 gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca acgtgaagta atctataaac
    12121 agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa gccactaatg aacatggtca
    12181 aacggacaat cagtcgtgta gtagatgcgc atacacaatt agagaaagaa aattggaatt
    12241 tggaaagtat catcgatttt gccggcagta ctcttgtcca tgaagatagt attactcttg
    12301 cagatatcga aggaaaaaca gctcaggaga tcaaagagta tctaaacgaa cgagcacaag
    12361 aagtatttaa taccaaagcg gctcaactaa atggtccaga gcaactgttg gaatttgaaa
    12421 aagtcgtgat cttgcgtgtt gtggactcta aatggacaga ccatattgat gcaatggatc
    12481 aattacgtca gtcaatcggc ttacgtgctt atggacagaa caatccatta gtcgaatatc
    12541 aaacagaagg ctataagatg tttgaagata tggtaggcgc catcgagtat gaagtcactc
    12601 gaatctttat gaaagctgag atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa
    12661 cagcttctca ctcagaagac ggagatgcca aagaaaatag taatactagc gcgaaaaaac
    12721 agccaatcca tgtgaacaag gtcggaagaa atgatccttg tccttgtgga agcgggaaaa
    12781 aatataagaa ctgtcatgga aaaaatgcaa actaatttct gacagcagga ttggtgagac
    12841 aaattctttt tttaagaatt cgtctcacct tctttctgtt atttgctgat tctgttagac
    12901 tagatataat gaaaaaagta tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg
    12961 aaatctcctg acagaaatgc aggaaaaggt cacaagcttc aggaggtctc tttgacttag
    13021 accagctgga agaagacatt gcggaagcag aagcaaaaat ggctgaacct ggtttttggg
    13081 atgatagcga agctgctcaa aaagtgatca gtgaaaacaa tgcaaataaa gaaacttatg
    13141 atcaattcca ttctcttgct gaagaactgg aagaaatcga attgatggtg gagatgttgc
    13201 aggaagaacc tgatgcagcc atgcaagaag aagcagaaac acgtatcaat gcgctagatg
    13261 aaaaaatgaa gacatatgaa ttatctatgt tgttagatgg tccttatgat cgaaacaacg
    13321 ccatcgttga attgcatcca ggggctggcg gaacggagtc ccaagattgg ggcagtatgc
    13381 ttttacgtat gtatacacgc tgggcagagc agcatgggta tcaagtagag acactagatt
    13441 atcaagcagg tgatgaagca ggaatcaaga gtgtgaccct actgatcaaa ggacacaacg
    13501 catttggtta tctgaaatca gaaaaaggtg ttcatcgcct tgtacgtatc tcgccttttg
    13561 actcagcgaa acgacgtcat acttcttttt gttcggtaga tgtgatgcca gagcttgatg
    13621 aaacgatcga tatagaaatc aatccagatg atttgaaaat cgatacgtac cgagcaagtg
    13681 gtgcaggtgg tcagcacatc aataaaacag aatctgctgt acggatcact cacttgccta
    13741 caggaacagt agtagctagt caggcacaac gttcacaatt gaaaaaccgt gagcaagcga
    13801 tggggatgtt aaaagctaag ctttatcaat tagaagtgga aaagaaagaa caagaagcag
    13861 ctgcacttcg aggagaacaa aaagaaatcg gctggggttc acagatccgt tcttatgttt
    13921 tccatccata ttctatggta aaagaccatc ggacaaacgt agagacagga aatgtccaag
    13981 cagttatgga cggtgacctt gatttattta ttgacggcta tttaaaaatg catctacaat
    14041 aagaacccat tgttaaattg aaataaaatt gtaagatttt gcaagcatgt tgaaacattg
    14101 ccatgctata atagctaaag actgaaaaag atatggagag aacgccatga ttgaaatgaa
    14161 ggatgtaatg aagaagtact caaatggtac gaccgctatc cgcaatctct caattgtcat
    14221 cgaacaagga gagtttgtct atgtggttgg tccgtctgga gccggtaaat ctacattcat
    14281 caaattgatg tatcgagaag aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga
    14341 tctaatagca atcaaaaacc gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt
    14401 ccaagactat aaattattac caagaaaaac agtatatgaa aatgttgcgt acgctatgca
    14461 agttatcggc agaaaacctc gggatatcaa aaaacgagta atggaagtgc tggaccttgt
    14521 tggtttgaaa cacaaagttc gagtctttcc tagcgaatta tccggtgggg aacagcaacg
    14581 tgtctctatt gctcgggcaa tagtgaatac gcctaaagta cttatcgcgg atgaaccgac
    14641 aggaaatctg gatccagaaa actcatggga aatcatgaaa ttattagatc ggatcaatgc
    14701 ccaaggaact actgtcgtga tggctacaca taacagtacg atcgtaaata cgattcggca
    14761 ccgagtaatt gctatcgaaa acggccgcat tattcgagat caagcggaag gggaatatgg
    14821 atacgatgat tagaaccttt ttccgccatt tattagaaag tatcaagagt ttgaaacgta
    14881 acggctggat gacagttgct tcagtcagtg cggtaacgat cacattgact ttagtcggtg
    14941 tattcatggc agttatcatg aacgcaacta aattagctca agatatagaa ggaaacgttg
    15001 atgtctctgt attcgtagat atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag
    15061 aattgaatga cttagatcat gttaaaaaag ttgaattttc aagcaaagaa caggaattga
    15121 aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt tgaaggtgac aataacccgt
    15181 tatatgatgt gtatgtagta agtgctactg atccttctta tacaaaacaa gtagcagaag
    15241 aagcagcaga tttgaagaat gtttctcgtt ctgattacgg tggagcttcc tcagatcgga
    15301 ttttccaaat cgctggtgca gttcggacat ggggattagt tgcagcggca ttgctgctgt
    15361 tcgtagcgat gtttctcatt tcgaacacga ttcggatcac gattttgtct agacagcgtg
    15421 aaattcaaat catgcgttta gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc
    15481 ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc gattttgatc atcacatttg
    15541 gttatgcatg gtttttccaa ttgatcaatc catcattatt acgttcccat tattcattga
    15601 ttcatccaca aaatatcgtt tggaaaatca atcttttaat ggtcggcatt ggtgtgatca
    15661 ttggttcact tggctcgatc atttctatga gacgtttctt gaaattctaa taccacaata
    15721 taaataaaat gagaaagttg gacagtttcg ttccagcttt ctttttttat tggctaggag
    15781 aatagaagga agcggctttt ttccctttac tttaaaaaac aacttgggta ttcttaagtt
    15841 ggtagaagaa gaggtgaagc cgtgaaaatt atcgttgaca atttttttac gatccttttg
    15901 attatgaata ttttgctttc ctttatcatc gtttttcgag aaagaaagga aactgctcaa
    15961 acatgggcat gggt
//
LOCUS       DAXWLB010000055        15468 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXWLB010000055 DAXWLB010000000
VERSION     DAXWLB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.055"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            135..3302
                     /locus_tag="ACGE4H_001372"
     CDS             135..3302
                     /locus_tag="ACGE4H_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFF8364798.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3370..3867
                     /locus_tag="ACGE4H_001373"
     CDS             3370..3867
                     /locus_tag="ACGE4H_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFF8364799.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            3886..4350
                     /locus_tag="ACGE4H_001374"
                     /pseudo
     CDS             3886..4350
                     /locus_tag="ACGE4H_001374"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            4347..5105
                     /locus_tag="ACGE4H_001375"
     CDS             4347..5105
                     /locus_tag="ACGE4H_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFF8364800.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(5191..5931)
                     /locus_tag="ACGE4H_001376"
     CDS             complement(5191..5931)
                     /locus_tag="ACGE4H_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFF8364801.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5992..7245
                     /locus_tag="ACGE4H_001377"
     CDS             5992..7245
                     /locus_tag="ACGE4H_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFF8364802.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7567..7794
                     /locus_tag="ACGE4H_001378"
     CDS             7567..7794
                     /locus_tag="ACGE4H_001378"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8364803.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7917..8243
                     /locus_tag="ACGE4H_001379"
     CDS             7917..8243
                     /locus_tag="ACGE4H_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8364804.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            8311..9024
                     /locus_tag="ACGE4H_001380"
     CDS             8311..9024
                     /locus_tag="ACGE4H_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8364805.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            9334..10185
                     /gene="licT"
                     /locus_tag="ACGE4H_001381"
     CDS             9334..10185
                     /gene="licT"
                     /locus_tag="ACGE4H_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFF8364806.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            10306..12171
                     /locus_tag="ACGE4H_001382"
     CDS             10306..12171
                     /locus_tag="ACGE4H_001382"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8364807.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            12185..13628
                     /locus_tag="ACGE4H_001383"
                     /pseudo
     CDS             12185..13628
                     /locus_tag="ACGE4H_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13736..14437
                     /locus_tag="ACGE4H_001384"
     CDS             13736..14437
                     /locus_tag="ACGE4H_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8364808.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14563..15075
                     /locus_tag="ACGE4H_001385"
                     /pseudo
     CDS             14563..15075
                     /locus_tag="ACGE4H_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5176 a         2630 c         3351 g         4311 t
ORIGIN      
        1 tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata catattttaa
       61 atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca aaacgtcaga
      121 gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt ttgtttgtct
      181 tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac gaatcaacgc
      241 aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt acttctgaaa
      301 ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat aagacaagtg
      361 agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg gcaagagctg
      421 tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag cttctcgcag
      481 acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg gaattagaga
      541 tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc acacaccata
      601 tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga acagctgcac
      661 atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct tatatgaccg
      721 atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg acgcttgatt
      781 tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc acattagatg
      841 gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc gctccgggta
      901 aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa ctttatggac
      961 cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt agtggaaaaa
     1021 cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat gatcgggcag
     1081 atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc atgtttgaag
     1141 tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc aatgacaaca
     1201 atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg aatggtggga
     1261 aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat tctagtcaaa
     1321 tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac gatcaacccg
     1381 ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa ggagccggat
     1441 cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt gagaatggta
     1501 cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca ggtgatggca
     1561 aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat gacgcttctg
     1621 gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa tcaaatatcg
     1681 atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat ttaaatttta
     1741 cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat aagaccggca
     1801 tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt gatttccaaa
     1861 acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa ggaaatggag
     1921 attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca ggcgcaacat
     1981 tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta tcgatcaatg
     2041 ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat gaatttgctc
     2101 agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag ctcccgatga
     2161 ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat gtcgttgttc
     2221 ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa gtgactttac
     2281 gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc acgcaaggac
     2341 aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa caaggcggct
     2401 tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa gtgcaagttg
     2461 ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct gccaaaacga
     2521 cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc aatgcagctt
     2581 ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa aaaaatcaag
     2641 ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct atcccagaaa
     2701 atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca ggagaagcta
     2761 ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat cacaatccaa
     2821 gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg aagatcgtag
     2881 tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag atcaaagcaa
     2941 ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta tggatttatg
     3001 atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag cctttcacaa
     3061 atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag gagtatcaat
     3121 tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat gtgcaagcag
     3181 attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc aaagaaaatc
     3241 agatagctgg taattactca gcagagataa catggacatt agcagatgcg ccgacagaat
     3301 aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg aaacaactgc
     3361 tctttactta tgatccttct ccttggattc atcctttggc agacacctgt ccatgcctcc
     3421 gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa gaacaaaaca
     3481 tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac ggtctttaac
     3541 cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac gaattcaaac
     3601 ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag ttatgatatg
     3661 aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc ggtcactgag
     3721 acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg agggatcacg
     3781 tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt caatcagttt
     3841 tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc agatttgaaa
     3901 ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc gaatcttcaa
     3961 aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta caaaaaagaa
     4021 acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc caatacaaat
     4081 tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta tcaagcggtt
     4141 gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga aataactgat
     4201 gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc agaaacattt
     4261 gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc tggattattt
     4321 attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt accgttctag
     4381 ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc tcagccacta
     4441 cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac gttgttccag
     4501 ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa ccaaatcagg
     4561 gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga acaatcaagt
     4621 taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt actggagcac
     4681 aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat gccggttgga
     4741 aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat aaactaactg
     4801 gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat agcagtggaa
     4861 tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca gatcaggcag
     4921 tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat tcattaggag
     4981 agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc aattatgctg
     5041 tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca gaagaggaag
     5101 cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac tccctgaaat
     5161 ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt ggaagtcatg
     5221 gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt caccgttgaa
     5281 aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt tgatacagta
     5341 agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt gttttaaaga
     5401 agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt tcaaaccttt
     5461 gatactttca atatataaaa tttcccgagg atacaggacg agacttccac ctttttgatc
     5521 aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct gttctagcac
     5581 ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg gaaggatttt
     5641 ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa tattactatg
     5701 tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt gcttcaagtc
     5761 gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca actgattgat
     5821 ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca attctctgat
     5881 gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca tatgcaggct
     5941 cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa gatggtcgca
     6001 acagtgacac tttatttgac ctttactttg attttagatt taggtatcta tttattcttt
     6061 tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt ggtgacactt
     6121 ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt gatgtttctt
     6181 ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct tttttttgct
     6241 atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg aaagagacat
     6301 cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct attagtaaaa
     6361 agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac tgaacactat
     6421 tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt gggaagtgtt
     6481 cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat cagttttttt
     6541 tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca acatcaggaa
     6601 tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga attcaaacat
     6661 gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa aaaagaaacg
     6721 ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa accgcaaata
     6781 caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa atttctagaa
     6841 aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt gaaaacaatc
     6901 ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa cgcattcgat
     6961 catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa agagggatca
     7021 cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga gatggtcaaa
     7081 agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa aaaaatgttg
     7141 gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca ctattttttt
     7201 gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac tgtcaaagga
     7261 taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt caaaagaaac
     7321 agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta aacaaagtag
     7381 ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact aagtagataa
     7441 gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta caccagataa
     7501 agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc tacgatcaca
     7561 aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat ccatcgaaaa
     7621 gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg aaaatcatta
     7681 agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa acaaaaagcc
     7741 tcaagggaag aagacacgct actgcataca catggtggct acctaaatta ttgattttct
     7801 atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca tctatttttt
     7861 atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc aagcaaatga
     7921 atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa tttcataacg
     7981 aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa ctttttcaat
     8041 ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa gaactggcag
     8101 atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta gatgagatca
     8161 ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc gcaggtgtac
     8221 gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga gcaaaagaaa
     8281 aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc aaaaagatgg
     8341 ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat tttcaatgaa
     8401 cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc gaatgtaaca
     8461 aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga tgcggtagaa
     8521 ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct accagtgatt
     8581 gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg cttggataat
     8641 acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg aaaaggaaat
     8701 tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc gctagaaaat
     8761 ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac atacaaaaca
     8821 ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt cgagggaaaa
     8881 gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact agttgtacaa
     8941 ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag atctgctttc
     9001 aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga ttaaaaacag
     9061 ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga aacgcaaaga
     9121 ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt tttatttttt
     9181 atatgaaaag gctcgcataa ataaaaattc gatggtatga tgattttgaa taagaaatgg
     9241 attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg cgaattctag
     9301 gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat tttgaataac
     9361 aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg aaaaggacta
     9421 gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa agaatttgtt
     9481 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
     9541 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
     9601 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
     9661 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
     9721 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
     9781 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
     9841 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
     9901 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
     9961 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    10021 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    10081 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    10141 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    10201 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    10261 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    10321 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    10381 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    10441 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    10501 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    10561 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    10621 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    10681 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    10741 gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    10801 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    10861 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    10921 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    10981 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    11041 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    11101 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    11161 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc
    11221 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    11281 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    11341 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    11401 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    11461 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    11521 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    11581 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    11641 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    11701 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    11761 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    11821 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    11881 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    11941 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    12001 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
    12061 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    12121 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    12181 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    12241 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    12301 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    12361 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    12421 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    12481 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    12541 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    12601 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    12661 atgaacgatt tgctcagaaa gtattagaga gatacagcag taaagtcaaa tattggatga
    12721 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    12781 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    12841 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    12901 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    12961 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    13021 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    13081 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    13141 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    13201 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    13261 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    13321 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    13381 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    13441 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    13501 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    13561 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    13621 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    13681 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    13741 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    13801 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    13861 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    13921 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    13981 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    14041 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    14101 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    14161 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    14221 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    14281 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    14341 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    14401 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    14461 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    14521 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    14581 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    14641 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    14701 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    14761 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    14821 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    14881 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    14941 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    15001 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    15061 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    15121 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    15181 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    15241 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    15301 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    15361 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    15421 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtga
//
LOCUS       DAXWLB010000056        15157 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXWLB010000056 DAXWLB010000000
VERSION     DAXWLB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.056"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(17..715)
                     /locus_tag="ACGE4H_001386"
                     /pseudo
     CDS             complement(17..715)
                     /locus_tag="ACGE4H_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            794..970
                     /locus_tag="ACGE4H_001387"
                     /pseudo
     CDS             794..970
                     /locus_tag="ACGE4H_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1146..1715)
                     /locus_tag="ACGE4H_001388"
     CDS             complement(1146..1715)
                     /locus_tag="ACGE4H_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8364809.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1789..3078)
                     /locus_tag="ACGE4H_001389"
     CDS             complement(1789..3078)
                     /locus_tag="ACGE4H_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364810.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3405..4280
                     /locus_tag="ACGE4H_001390"
     CDS             3405..4280
                     /locus_tag="ACGE4H_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8364811.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4294..4617
                     /locus_tag="ACGE4H_001391"
     CDS             4294..4617
                     /locus_tag="ACGE4H_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364812.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4614..5366
                     /locus_tag="ACGE4H_001392"
     CDS             4614..5366
                     /locus_tag="ACGE4H_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFF8364813.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5356..7527
                     /locus_tag="ACGE4H_001393"
                     /pseudo
     CDS             5356..7527
                     /locus_tag="ACGE4H_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACGE4H_001394"
     CDS             complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACGE4H_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFF8364814.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9830..12016)
                     /locus_tag="ACGE4H_001395"
     CDS             complement(9830..12016)
                     /locus_tag="ACGE4H_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8364815.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACGE4H_001396"
     CDS             complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACGE4H_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFF8364816.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12238..12678)
                     /locus_tag="ACGE4H_001397"
     CDS             complement(12238..12678)
                     /locus_tag="ACGE4H_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFF8364817.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12752..13250)
                     /locus_tag="ACGE4H_001398"
                     /pseudo
     CDS             complement(12752..13250)
                     /locus_tag="ACGE4H_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13535..15001)
                     /locus_tag="ACGE4H_001399"
     CDS             complement(13535..15001)
                     /locus_tag="ACGE4H_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8364818.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4311 a         3161 c         2683 g         5002 t
ORIGIN      
        1 aagagggggg gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat
       61 cttgtgctcc acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata
      121 ctttcttatt agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag
      181 tattataagc ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt
      241 cttttccagt attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc
      301 atccagctac atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat
      361 ttgttccatc tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt
      421 tatcctccga agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt
      481 tttctttact tacacccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa
      541 tattttgtgt tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg
      601 ttaatggaat accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt
      661 tcttttcaaa agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg
      721 ctcacgaggt tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac
      781 attatgtttt gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa
      841 agatttttta ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga
      901 agtgaaaaaa agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca
      961 ctcaaattga gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata
     1021 agcgaacatg tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc
     1081 gttcgttcaa ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg
     1141 ttttcttata gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat
     1201 tgactgtttt gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt
     1261 aactggtagt tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca
     1321 aatccggtct ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct
     1381 gtaatcgtca caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct
     1441 tcagcattgg atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt
     1501 tctttcctct ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgttttg
     1561 tattgttctt gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca
     1621 gttcccgcgt ctcgcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc
     1681 gaagatgtgg gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct
     1741 ttttattata gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt
     1801 gcctaaccag taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag
     1861 atacgttttt tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga tcactagatc
     1921 tgcataccga ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct
     1981 tagcgttttg ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag
     2281 tacttttatc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagtaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgagttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
     3181 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     3241 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
     3301 aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
     3361 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
     3421 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     3481 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     3541 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     3601 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg
     3661 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3721 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3781 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
     3841 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3901 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3961 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     4021 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     4081 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     4141 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     4201 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     4261 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     4321 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     4381 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     4441 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     4501 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     4561 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     4621 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     4681 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4741 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4801 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4861 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4921 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4981 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     5041 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     5101 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     5161 aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     5221 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggcttatc
     5281 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     5341 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     5401 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     5461 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     5521 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     5581 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     5641 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     5701 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     5761 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     5821 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5881 atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5941 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     6001 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     6061 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     6121 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     6181 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     6241 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     6301 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     6361 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     6421 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     6481 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     6541 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     6601 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     6661 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccgaaagaa
     6721 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6781 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6841 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6901 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6961 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     7021 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     7081 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     7141 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     7201 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     7261 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     7321 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     7381 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat
     7441 aaaaaaacct atttagaaat aacgcgtata ataagaaaaa cggtatacaa aaaaattctg
     7501 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     7561 ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt
     7621 cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac
     7681 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7741 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7801 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7861 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7921 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7981 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     8041 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     8101 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     8161 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     8221 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     8281 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac
     8341 tgttgcctct agcccgactc ctgaaagatt ttttagattt tcagcttgat aaggcttgat
     8401 ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc
     8461 caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     8521 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     8581 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     8641 tattgatgtg gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     8701 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8761 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8821 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8881 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8941 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     9001 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     9061 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     9121 ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt
     9181 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     9241 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     9301 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     9361 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     9421 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     9481 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     9541 agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc
     9601 catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     9661 atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat
     9721 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc
     9781 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9841 tgtgtcgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9901 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9961 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga
    10021 ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg
    10081 caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat
    10141 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
    10201 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
    10261 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
    10321 ctttattctg gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg
    10381 caatccaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
    10441 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
    10501 tacgtgtacc cgcaaaatag cgtactccat taatcgtacc gctgatccca gcacctgggt
    10561 gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
    10621 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
    10681 cttttgaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat
    10741 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10801 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggggtc gctaaaccta
    10861 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10921 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10981 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    11041 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca
    11101 tgtgtagcag accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt
    11161 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    11221 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    11281 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    11341 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    11401 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    11461 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    11521 ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa
    11581 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    11641 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    11701 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11761 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccataaccga
    11821 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11881 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11941 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    12001 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    12061 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    12121 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    12181 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    12241 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    12301 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtagcttc
    12361 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    12421 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    12481 tcctttttgt tctgtccaga tcacttcttt ttttaccaaa cgtcccagca aagttttgat
    12541 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    12601 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg
    12661 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attagcttat
    12721 gaatacagat tacgcttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12781 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12841 tagctacttg gatcagctgc tgtatttttg gttcattgtt tccttcaaaa tgccagataa
    12901 aagataaaaa ttctaagtat ttcctgttta gtttctccga aaatgattca ggcatatctg
    12961 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    13021 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaacg
    13081 tagaagtata atttccttca acgatccatg acggattaga tactataaac gattgctgtt
    13141 cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt
    13201 gcgagggacg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    13261 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    13321 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa atggtgttca atcagctgac
    13381 cttcagcatt agctcaaaaa acggcaaaat atgaggagct atttttgttt ggctgttctt
    13441 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc agaagtagct
    13501 tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact gtttctggtt
    13561 cagtaatttc tttgtttttg tctcgacgag caattgctgg caagatcata cctaataaga
    13621 tcaagacaat tggtgtgatg atattcgtga ataattggaa ccaccatgct ttaggatcag
    13681 cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg aaggcaaagc
    13741 accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat tcagaattga
    13801 atttcttata agctttattc aacatcatat atgccaagaa cacccatagg taacgcattg
    13861 gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata tctttgatac
    13921 caaagatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga taaccattca
    13981 ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct ggatccgcat
    14041 ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag atttgtccaa
    14101 tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag taattaccta
    14161 aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttagggata ttatcgctag
    14221 caaacagcat acccattgcg actgagccta atacagcaca aatccctacc attgctgcta
    14281 agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg acataagggg
    14341 atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg aaataactga
    14401 aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca aattccatcg
    14461 ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac atgacgaaca
    14521 tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt gttgataaat
    14581 ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact gacatcgtat
    14641 ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt gcttgaggct
    14701 tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag tatgccagtc
    14761 gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta tccttgaagg
    14821 ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc aaaatcaata
    14881 gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca ttccccatgc
    14941 cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt ttgttgttca
    15001 ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt caatttttaa
    15061 caaacttaca agacagtgtt attttatgtg tgtattattt tatttaatac acatcgtttt
    15121 ttcatatttt ttccatcaaa aaaataagtt attttct
//
LOCUS       DAXWLB010000057        15141 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXWLB010000057 DAXWLB010000000
VERSION     DAXWLB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.057"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4H_001400"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACGE4H_001400"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFF8364819.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4H_001401"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACGE4H_001401"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFF8364820.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4H_001402"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACGE4H_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFF8364821.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4H_001403"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACGE4H_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFF8364822.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4H_001404"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACGE4H_001404"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFF8364823.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4H_001405"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACGE4H_001405"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFF8364824.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACGE4H_001406"
     CDS             complement(8054..8524)
                     /locus_tag="ACGE4H_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFF8364825.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACGE4H_001407"
     CDS             complement(8570..8995)
                     /locus_tag="ACGE4H_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFF8364826.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACGE4H_001408"
     CDS             complement(9062..9331)
                     /locus_tag="ACGE4H_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFF8364827.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACGE4H_001409"
     CDS             complement(9342..10937)
                     /locus_tag="ACGE4H_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8364828.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4H_001410"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACGE4H_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFF8364829.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4H_001411"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACGE4H_001411"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFF8364830.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3907 a         3428 c         2686 g         5120 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac t
//
LOCUS       DAXWLB010000058        15100 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXWLB010000058 DAXWLB010000000
VERSION     DAXWLB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.058"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(21..251)
                     /locus_tag="ACGE4H_001412"
     CDS             complement(21..251)
                     /locus_tag="ACGE4H_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364831.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="ACGE4H_001413"
     CDS             complement(687..1253)
                     /locus_tag="ACGE4H_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8364832.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="ACGE4H_001414"
     CDS             complement(1253..2209)
                     /locus_tag="ACGE4H_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFF8364833.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="ACGE4H_001415"
     CDS             complement(2209..2865)
                     /locus_tag="ACGE4H_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFF8364834.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="ACGE4H_001416"
     CDS             3235..5253
                     /locus_tag="ACGE4H_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8364835.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE4H_001417"
     CDS             5680..8094
                     /gene="leuS"
                     /locus_tag="ACGE4H_001417"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFF8364836.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="ACGE4H_001418"
     CDS             8442..9995
                     /locus_tag="ACGE4H_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFF8364837.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="ACGE4H_001419"
     CDS             complement(10376..12160)
                     /locus_tag="ACGE4H_001419"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364838.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="ACGE4H_001420"
     CDS             complement(12175..13896)
                     /locus_tag="ACGE4H_001420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364839.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="ACGE4H_001421"
     CDS             13996..14757
                     /locus_tag="ACGE4H_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8364840.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4906 a         2771 c         2978 g         4445 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt
//
LOCUS       DAXWLB010000059        15028 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXWLB010000059 DAXWLB010000000
VERSION     DAXWLB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.059"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            130..612
                     /locus_tag="ACGE4H_001422"
     CDS             130..612
                     /locus_tag="ACGE4H_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364841.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            809..1699
                     /locus_tag="ACGE4H_001423"
     CDS             809..1699
                     /locus_tag="ACGE4H_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFF8364842.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2025..4499
                     /locus_tag="ACGE4H_001424"
     CDS             2025..4499
                     /locus_tag="ACGE4H_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8364843.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4602..6338)
                     /locus_tag="ACGE4H_001425"
     CDS             complement(4602..6338)
                     /locus_tag="ACGE4H_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8364844.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6335..7039)
                     /locus_tag="ACGE4H_001426"
     CDS             complement(6335..7039)
                     /locus_tag="ACGE4H_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8364845.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7170..7673
                     /locus_tag="ACGE4H_001427"
     CDS             7170..7673
                     /locus_tag="ACGE4H_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFF8364846.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7633..7971
                     /locus_tag="ACGE4H_001428"
     CDS             7633..7971
                     /locus_tag="ACGE4H_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFF8364847.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7992..9410
                     /gene="ffh"
                     /locus_tag="ACGE4H_001429"
     CDS             7992..9410
                     /gene="ffh"
                     /locus_tag="ACGE4H_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFF8364848.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9522..10100
                     /locus_tag="ACGE4H_001430"
     CDS             9522..10100
                     /locus_tag="ACGE4H_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFF8364849.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10104..10625
                     /locus_tag="ACGE4H_001431"
     CDS             10104..10625
                     /locus_tag="ACGE4H_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFF8364850.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10704..11258)
                     /locus_tag="ACGE4H_001432"
     CDS             complement(10704..11258)
                     /locus_tag="ACGE4H_001432"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8364851.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11511..11786
                     /gene="rpsP"
                     /locus_tag="ACGE4H_001433"
     CDS             11511..11786
                     /gene="rpsP"
                     /locus_tag="ACGE4H_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFF8364852.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11798..12049
                     /locus_tag="ACGE4H_001434"
     CDS             11798..12049
                     /locus_tag="ACGE4H_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFF8364853.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12173..12964
                     /locus_tag="ACGE4H_001435"
     CDS             12173..12964
                     /locus_tag="ACGE4H_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364854.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13134..13655
                     /gene="rimM"
                     /locus_tag="ACGE4H_001436"
     CDS             13134..13655
                     /gene="rimM"
                     /locus_tag="ACGE4H_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFF8364855.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13645..14406
                     /gene="trmD"
                     /locus_tag="ACGE4H_001437"
     CDS             13645..14406
                     /gene="trmD"
                     /locus_tag="ACGE4H_001437"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFF8364856.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14542..14889
                     /gene="rplS"
                     /locus_tag="ACGE4H_001438"
     CDS             14542..14889
                     /gene="rplS"
                     /locus_tag="ACGE4H_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFF8364857.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
BASE COUNT         4969 a         2728 c         3108 g         4223 t
ORIGIN      
        1 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
       61 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
      121 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
      181 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
      241 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
      301 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
      361 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
      421 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
      481 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
      541 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
      601 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
      661 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
      721 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
      781 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
      841 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
      901 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
      961 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     1021 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     1081 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     1141 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     1201 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     1261 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     1321 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     1381 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     1441 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     1501 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     1561 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     1621 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     1681 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     1741 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     1801 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     1861 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     1921 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     1981 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     2041 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     2101 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     2161 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     2221 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     2281 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     2341 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     2401 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     2461 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     2521 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     2581 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     2641 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     2701 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     2761 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     2821 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     2881 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     2941 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     3001 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     3061 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     3121 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     3181 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     3241 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     3301 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     3361 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     3421 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     3481 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     3541 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     3601 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     3661 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     3721 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     3781 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     3841 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     3901 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     3961 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     4021 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     4081 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     4141 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     4201 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     4261 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     4321 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     4381 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     4441 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     4501 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     4561 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     4621 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     4681 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     4741 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     4801 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     4861 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     4921 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     4981 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     5041 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     5101 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     5161 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     5221 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     5281 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     5341 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     5401 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     5461 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     5521 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     5581 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     5641 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     5701 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     5761 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     5821 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     5881 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     5941 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     6001 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     6061 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     6121 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     6181 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     6241 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     6301 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     6361 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     6421 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     6481 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     6541 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     6601 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     6661 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     6721 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     6781 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     6841 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     6901 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     6961 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     7021 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     7081 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     7141 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     7201 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     7261 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     7321 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     7381 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     7441 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     7501 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     7561 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     7621 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     7681 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     7741 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     7801 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     7861 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     7921 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     7981 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     8041 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     8101 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     8161 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     8221 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     8281 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     8341 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     8401 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     8461 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     8521 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     8581 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     8641 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     8701 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     8761 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     8821 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
     8881 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
     8941 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
     9001 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
     9061 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
     9121 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
     9181 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
     9241 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
     9301 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
     9361 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
     9421 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
     9481 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
     9541 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
     9601 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
     9661 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
     9721 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
     9781 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
     9841 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
     9901 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
     9961 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    10021 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    10081 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    10141 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    10201 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    10261 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    10321 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    10381 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    10441 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    10501 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    10561 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    10621 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    10681 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    10741 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    10801 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    10861 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    10921 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    10981 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    11041 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    11101 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    11161 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    11221 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    11281 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    11341 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    11401 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    11461 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    11521 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    11581 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    11641 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    11701 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    11761 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    11821 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    11881 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    11941 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    12001 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    12061 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    12121 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    12181 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    12241 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    12301 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    12361 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    12421 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    12481 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    12541 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    12601 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    12661 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    12721 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    12781 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    12841 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    12901 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    12961 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    13021 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    13081 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    13141 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    13201 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    13261 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    13321 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    13381 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    13441 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    13501 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    13561 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    13621 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    13681 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    13741 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    13801 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    13861 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    13921 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    13981 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    14041 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    14101 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    14161 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    14221 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    14281 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    14341 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    14401 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    14461 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    14521 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    14581 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    14641 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    14701 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    14761 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    14821 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    14881 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    14941 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    15001 ataatggatt agaataaatc tacctata
//
LOCUS       DAXWLB010000060        14987 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXWLB010000060 DAXWLB010000000
VERSION     DAXWLB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.060"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4H_001439"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACGE4H_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFF8364858.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACGE4H_001440"
     CDS             complement(322..891)
                     /locus_tag="ACGE4H_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFF8364859.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACGE4H_001441"
     CDS             1077..1979
                     /locus_tag="ACGE4H_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8364860.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4H_001442"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACGE4H_001442"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFF8364861.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4H_001443"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACGE4H_001443"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFF8364862.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4H_001444"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACGE4H_001444"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFF8364863.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACGE4H_001445"
     CDS             8547..8732
                     /locus_tag="ACGE4H_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFF8364864.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACGE4H_001446"
     CDS             complement(8794..9990)
                     /locus_tag="ACGE4H_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFF8364865.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACGE4H_001447"
     CDS             complement(10160..11596)
                     /locus_tag="ACGE4H_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFF8364866.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACGE4H_001448"
     CDS             11889..12698
                     /locus_tag="ACGE4H_001448"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8364867.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12884..14986)
                     /locus_tag="ACGE4H_001449"
     CDS             complement(12884..14986)
                     /locus_tag="ACGE4H_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFF8364868.1"
                     /translation="MRKGAQEPKNKTFRRSHVPFRLNFLFFIIFTLFVALIARLGYLQ
                     IVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAITYTRGTQVTAKDL
                     LEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAKEKQLGNSEQYSAM
                     VNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDRELAVVAERASELPG
                     VSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYSRNDRVGQSYLEKQ
                     YEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEFQSKVEEILKRNYQ
                     TLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENALGTITSAFDPGSV
                     VKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVALDAVKASELSSNT
                     YMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTTTGIDLPNESPGIS
                     RSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYASTVANGGKRVQPHLV
                     KAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVVHGTDPYTTAKPLA
                     SAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISVVLPNLLDEQDHIN
                     LTIAKEIMDAYYDMFMAK"
BASE COUNT         4376 a         3178 c         2660 g         4773 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt
    12841 ttcttcatac tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca
    12901 tatcataata cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt
    12961 ctaataagtt cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg
    13021 ccacgatatt actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag
    13081 tccctgtttt tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag
    13141 gatcggtacc atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag
    13201 aaatattgac cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc
    13261 cattttcgtc attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat
    13321 tagcgactgt tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa
    13381 atgccaaatc ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact
    13441 tcttcatata atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag
    13501 tcgttgtacc aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt
    13561 cttcatctaa actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg
    13621 ccactttgat catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa
    13681 cttgtccgct tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct
    13741 catcaatcaa aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac
    13801 cagccttgac tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg
    13861 tgatttcttt ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct
    13921 gaggattcat agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca
    13981 atgtttgata attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag
    14041 ataacattaa attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc
    14101 cttcattatc aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt
    14161 gtttttccaa ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt
    14221 tctctgcttc atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt
    14281 tcagtgaact tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa
    14341 gttcagaagc gcgttctgcc acaacagcta actctcgatc agtaacacca gagtttttga
    14401 tatagacagt gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta
    14461 attgctcctc gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt
    14521 gttcgctgtt ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat
    14581 gtttgggatc agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg
    14641 gaacatcgat cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt
    14701 gtgttcctct cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg
    14761 tggagtcgta gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact
    14821 tcactcgcgc ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca
    14881 acgcaacaaa taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg
    14941 accggcgaaa cgttttgttc ttaggttctt gtgcaccttt tcttatt
//
LOCUS       DAXWLB010000061        14844 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXWLB010000061 DAXWLB010000000
VERSION     DAXWLB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.061"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..270
                     /locus_tag="ACGE4H_001450"
     CDS             <1..270
                     /locus_tag="ACGE4H_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364869.1"
                     /translation="NSDSETIEDVKKIIVLFEKENMELENWGLFEIPISGNYCFYNWK
                     TEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAIKLERVRK"
     gene            272..535
                     /locus_tag="ACGE4H_001451"
     CDS             272..535
                     /locus_tag="ACGE4H_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364870.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSIVKEFLYSNPKIALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            717..1469
                     /locus_tag="ACGE4H_001452"
     CDS             717..1469
                     /locus_tag="ACGE4H_001452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364871.1"
                     /translation="MYYQLLYDKIIDAETFYYKEKNEWLNGLSLYLRFDPIFYVENKQ
                     KSKEQMIQLKNERSLLNRLVRVSYDNQTRKLSFHDHEEIKGKYRVAKYYKLVTDEEYS
                     GWCEEEKISKEEFYFLIQHYGQHFHNDFSLQSCSYGWHEIESLPEGTIVQNSYPKHLN
                     ETTKRLEEIVFEEKKRHEQLLSVKKGIMKFEYRSECLEFIKRIELLENHKDFLLNKEQ
                     FDQEEVMESYVQLYTEWIRFNRFMSQENLYTA"
     gene            1772..2749
                     /locus_tag="ACGE4H_001453"
     CDS             1772..2749
                     /locus_tag="ACGE4H_001453"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFF8364872.1"
                     /translation="MEDKKYSKIIKKIKGLLAIAKDENQDEESQSAFVLAQRLMLQYK
                     IDKNDVFDTEDEIEPIGEESVTIYKKLYWWERSLGSIIAENFRVKMFYNSKKEKGERK
                     SKSKIVFYGFGSDLELAKEMYFLAYEVIVFHTNQYIKNWYQDTDMKRSRYITESVKSS
                     YISGFLVGLQKRFEEQISVLAERYEVMVLIPKAVEESFDKYSKDFSSYSTNTPPVSVD
                     HAYQEGVREAKKIDLTKSTVDTDYSSLVGRIIRFNQGVTRGLIAEITSVSDDLLNLLV
                     MNCVSEYASEDNPSFYHWKVDKEYDYEILSFNDPMSKKFKKNKVDIMEN"
     gene            2901..3767
                     /locus_tag="ACGE4H_001454"
     CDS             2901..3767
                     /locus_tag="ACGE4H_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364873.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWEKVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYEPTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            3922..5748
                     /locus_tag="ACGE4H_001455"
     CDS             3922..5748
                     /locus_tag="ACGE4H_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFF8364874.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSIPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKVCENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            5756..6829
                     /locus_tag="ACGE4H_001456"
     CDS             5756..6829
                     /locus_tag="ACGE4H_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364875.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            6838..7098
                     /locus_tag="ACGE4H_001457"
     CDS             6838..7098
                     /locus_tag="ACGE4H_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364876.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            7135..8139
                     /locus_tag="ACGE4H_001458"
     CDS             7135..8139
                     /locus_tag="ACGE4H_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HFF8364877.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPISVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            8164..8520
                     /locus_tag="ACGE4H_001459"
     CDS             8164..8520
                     /locus_tag="ACGE4H_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364878.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            8682..8873
                     /locus_tag="ACGE4H_001460"
     CDS             8682..8873
                     /locus_tag="ACGE4H_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364879.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            8982..9200
                     /locus_tag="ACGE4H_001461"
     CDS             8982..9200
                     /locus_tag="ACGE4H_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364880.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKAIGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            9237..9554
                     /locus_tag="ACGE4H_001462"
     CDS             9237..9554
                     /locus_tag="ACGE4H_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364881.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRFIEVNNLGIIEDYLDKLDEAIKQSIEKNGEDHILTVVLKDLKDRLEAEAEYF
                     NKD"
     gene            9574..9762
                     /locus_tag="ACGE4H_001463"
     CDS             9574..9762
                     /locus_tag="ACGE4H_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364882.1"
                     /translation="MTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLTLSS
                     FVTRVDQKKVINQKDFKI"
     gene            9759..10727
                     /locus_tag="ACGE4H_001464"
     CDS             9759..10727
                     /locus_tag="ACGE4H_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFF8364883.1"
                     /translation="MSLENEKPYYVVVGGVNGAGKSTIYQVQNLKRKELDTDRINADE
                     ILRSFGGNWKKQADINKSMREVLREIKGHFKEGKSFHHETTFGGNPYPDKMRLLKAKK
                     LGYESFLIYIGLDSPERAIERVKIRVAKGGHGVPEDVIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYNWVPKAIQEKNAEIPISKQIIKDISQEGL
                     SPKETLERIYLASDDFISDRFFKAEQVDDFYMNHKNEIKERILAISFSTGVPEDELVG
                     QDKKRNSCKLAYINSVGELLGKTDSELSGMEVNRDRSIKKTDFEIE"
     gene            10971..12143
                     /locus_tag="ACGE4H_001465"
     CDS             10971..12143
                     /locus_tag="ACGE4H_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364884.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKVLNMVYRQLMFE
                     ELIKDAIKEMDQGYVEREKSIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFVGIVTGQRTPRVY
                     RNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGSKPRKARDSALTLGYIMDG
                     KYKLNQSNLIFEGNKLFFNLTISFEKDYHKLEFKEGSELTIKFTDLCSGECLVNNKSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVQTAIRNKAQIICISEPDKSYGEWAYYQLKEQIINKGKRYGIEVKSV"
     gene            12976..13587
                     /locus_tag="ACGE4H_001466"
     CDS             12976..13587
                     /locus_tag="ACGE4H_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8364885.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFNRVGVEKVFAEKKSGRNTAERP
                     EFNKMLAFVREGDTLVFESLERLGRNSDEILDTVSFIDKNGMSLMVLNLPILDTKFGD
                     PNLEKLVRSLVISIFSWTAQNEREEIKRKQKQGIAIAKRKGYYKGKPYEYSENAKNPQ
                     KRFIYNEIVRMLGEGLPIKKIAEKTGTNRNLVYRIKKRISESG"
     gene            13831..14061
                     /locus_tag="ACGE4H_001467"
     CDS             13831..14061
                     /locus_tag="ACGE4H_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364886.1"
                     /translation="MNQPRRKRLESISEKISELKDLLEEIKEEEEEYKENMPENLWGS
                     ERYERAEEAVGQLEEADDYLDQVIGLIEEVIS"
     gene            14115..14468
                     /locus_tag="ACGE4H_001468"
     CDS             14115..14468
                     /locus_tag="ACGE4H_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364887.1"
                     /translation="MTQNYIDNWRLFSGKLMTKDSIAEMPESLEKEHILKVREMLHKE
                     GFNADLFRVGELPIEGVYYFERAVSENGVDYFSIIVEDDGSLTPQYTTLECDESGFNT
                     FPCNTIQESIDKHEC"
     gene            14496..14804
                     /locus_tag="ACGE4H_001469"
     CDS             14496..14804
                     /locus_tag="ACGE4H_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364888.1"
                     /translation="MKLEIKEVVCDWGIYVDGQTYPFMIFNSKANAQEIMRIMELDNK
                     HERFDEVNKMENECEAVEYHKTCANNKYILGAMGLGKTASIKQVYEESQENCVYENSN
                     "
BASE COUNT         5885 a         1656 c         2977 g         4326 t
ORIGIN      
        1 aacagtgatt cggaaactat agaagatgta aaaaaaatca tagtgttgtt tgaaaaagaa
       61 aacatggagc tagagaattg ggggcttttt gaaataccga ttagtgggaa ttactgtttt
      121 tataattgga agacagaaga cgatgtagct tttgcaaatt acttttttga taaaaattac
      181 ttttccccat tatatataga taagcatagt aatgaacaag ttgcttcttc aataaaagaa
      241 gcaataaaat tggaaagagt gaggaaatga tatgatcgat tttagaacgg ttatggataa
      301 actaagtaat ggagattttc gcgtattgat tgaactatac ttaaatgtaa cgtctatagt
      361 caaagagttt ttatattcta atcccaaaat agctttagta ttagttgcag tactgatagt
      421 ttttgctgtg atctctttta ttaagaaaat gattccgcta ttaattatag gtgctgtgtt
      481 gatattaggt ttgtatttcc ctgaagtttt ggatcaaata aaaaatatat tttaggctct
      541 aaaagcttcc agtagaaaat tttttagatt acttgtacta acaacataag gtaaaaagga
      601 gaaaattgat gaaagtttct attgcaaaag aatcaattga tagaagagaa gctattatta
      661 gtgaactaaa aatcactgaa aaattgatga aagaaaaata gcgaaggagg tttaagatgt
      721 attatcaact gttgtacgac aaaataatag atgcagaaac gttttattat aaagaaaaaa
      781 acgaatggct taatggatta agtttatatc taagatttga tccaatcttt tatgtagaaa
      841 ataaacagaa atcaaaagaa caaatgattc agctaaaaaa cgagcgaagt ttattgaatc
      901 gcttagttcg tgtctcttat gacaatcaaa caagaaaatt atcgtttcat gaccacgagg
      961 agataaaagg aaaataccga gtggcaaaat attataaact agtcaccgat gaagagtatt
     1021 caggttggtg tgaagaagaa aaaattagta aggaagaatt ttattttctt atccaacatt
     1081 acggacaaca ttttcacaat gacttttctc tgcaatcctg ttcatatggt tggcatgaaa
     1141 tagaaagtct tcctgaagga acaattgttc aaaatagtta tccaaaacat ttaaatgaaa
     1201 cgacaaagcg tttggaagaa atcgtatttg aagagaaaaa acggcatgaa cagttgctaa
     1261 gtgttaaaaa agggataatg aaatttgaat accgatcaga atgtttagaa tttattaaac
     1321 gaatcgaact cttggaaaat cataaagact ttctattgaa taaggaacaa ttcgaccaag
     1381 aagaagtgat ggaatcttat gttcaattgt atacggaatg gattcgtttt aatcgattta
     1441 tgtcacaaga aaatctctac acagcttaat tttagagttt taaagcttcc ggtagaaaat
     1501 tttgggcttt gatatttgtt ttaagcggtt tgaaatagaa atttgataaa ctacatgaaa
     1561 agacattata tagtcgtgta gagcgaatta gtgggtttaa atatcgtttg actacttgta
     1621 gtcaagtgtg ttaagctatt tttgtagtaa taagacataa gaagttaaag gaagttattg
     1681 tacttccttt aatttaagct aaaaagacta caagtagtct tttcggtttt gagaggtgaa
     1741 aatatataat taaacttggt aggtgaagaa aatggaagac aagaagtatt caaaaattat
     1801 aaagaaaata aaagggcttt tagcaatagc gaaagatgaa aatcaggatg aagagagtca
     1861 atctgctttc gttttagcac aaaggcttat gttgcaatat aaaatagata aaaatgatgt
     1921 attcgatact gaagatgaaa tagaaccaat cggtgaagag tcagtaacga tctataagaa
     1981 actatattgg tgggaacgat cgctaggatc aataatagcc gagaatttta gggttaaaat
     2041 gttttacaat agtaaaaagg aaaaaggaga gaggaaaagt aaaagtaaaa tagtatttta
     2101 tggattcgga agtgatttag agttagctaa ggaaatgtat tttttagcgt atgaagttat
     2161 tgtatttcat actaatcaat atataaaaaa ctggtatcaa gatactgata tgaaaagaag
     2221 tagatatata acagaaagtg tgaaatcaag ctatatatcc ggatttttag taggtctgca
     2281 aaaacgattc gaggaacaaa tttctgtttt ggccgaacgg tatgaagtaa tggttttgat
     2341 tccaaaagct gtagaagaat cctttgataa atatagtaaa gatttttcta gttatagtac
     2401 taatactcca ccggtatctg tggatcatgc gtaccaagag ggagtaagag aagctaaaaa
     2461 aatagattta acaaaaagca ctgttgatac agattatagt agtttagtag gtcgtattat
     2521 taggtttaat caaggagtaa ctcgtggatt aatagctgaa atcactagtg tttctgacga
     2581 tctattaaat ttgttggtaa tgaattgtgt ttcagaatat gcttctgaag ataacccttc
     2641 tttctatcat tggaaagtag ataaggagta cgactatgaa atcctttctt tcaacgatcc
     2701 catgagtaaa aagtttaaaa aaaataaagt agacataatg gaaaactaaa aaaagtgata
     2761 aacaaaggac tgattatttg gttctttgtt ttttgctata tttttctaca ggcaattatc
     2821 ttaagagtat ctgtgtattg gaataaaaat atgataatat gaaattatgt atcagaccga
     2881 taaaaaagag gtgcttataa atgaaaaagt taagagttat tgcaacaata cctatgattt
     2941 ttagttttgc gctattatta tctgcatgtg gtaaagaaga agctggtgct aagtcaaatg
     3001 aagcacataa tatctcattc atagatagtg cagaacaaaa agattcacgt tgttggttgg
     3061 tagtcgaaac aagtagtaaa aataaaacgc ctattgataa gaacgaatat gtaaaagcgg
     3121 tgatccaaac taaagatggc aaagcaaaag tatttaaaac ggatatggaa cagcttacat
     3181 tggaaaaagt ttctaaaatg agtgatgaag aaataataga acaagcattg aacgctgata
     3241 aacaaaagtt taatgataag aaaaaaagcg atattgatag ctacaatgct actttagatt
     3301 ctgtaaaaac agaattagaa aatgaggaaa aacaagagtt tcaaaatgac caaaaaattg
     3361 ctgattttca agatagtata cctaaaatac aggcggatat agaagagatc gatggtgttc
     3421 aatattcaga accggagtgg gaaaaggtat cagcaacaat taaaatggat aatgaaaaca
     3481 aatcaccttc aatggaaacc gttaaattat cttataaata tttagatagt ccaatttgga
     3541 atttaaatag ttttggtgaa gtttcgtctg aaggagaatt atcttatgaa cctactagta
     3601 tggatttgaa gttgtcaatt ccagttgcca caacgaaagt tttagataat ttttattcgg
     3661 gatattctaa tgatgaattg gatcaacttt tgcttactaa agtagcttct aaagataaca
     3721 aagttcaatt agatagtaaa ggtaaaaacg taagtatagt tgagtagatg gtaaaataag
     3781 aagggataag atggttacta taactatctt atctcttttt tatttgcatt aagtcagtta
     3841 aattagatag aagagttttt atatatctgg tataatttaa ttaggtaatt taacttacct
     3901 aaaaaagaaa ggagatagaa gatggcctat aaaaaaggaa actggaaacc gaaaacgaaa
     3961 gagcaaaaac aaaaagagct agacgattta attaaggctt ccaacagcaa aatcgagcaa
     4021 tatcagtcta accctgaaga tatgatcgag tttgccaagt tcatgtctaa aatacacaat
     4081 tacagtcctt taaacctatc gttgatcgat gagggatttg aaggagcaat tgctgtagca
     4141 agttatgatg gttggaaaaa actaggattt caagttaaag cgaaagaaaa agggataggc
     4201 atttatgcgc atgctccggt aacggttttt attgatgaaa atggtgagga aaagacgtta
     4261 aaagaagcga caagcgagca aaaagaaaaa atcaaaaatg gagaattaag aagcaaaaag
     4321 atcaagcatt ttaagaaggg ttacgtattt gatatttccc aaactaatgc aactgaagct
     4381 gatttgccaa aaatatttcc taacagggta tggaatttta atattgatgg tgaattaagt
     4441 gttagtcaat tagaaaaagg tgtaaatcat ttggccgaat ctatgaatat atctataaaa
     4501 gatatgaatg agtctggtag aggtgaaatg ggaagcgcta gaggtgaata tattcaatat
     4561 gttgatggta gggaagaaat tgtattaaat tcaagaaata gcagaacaca aaatcttact
     4621 accagtattc acgaattggc tcacaaaaag cttcacaata agaatgaaca cggttcttcc
     4681 tactcaatac cgattaaaga gtttcaagcg gaaatgactt catttattgt ttgtcacaat
     4741 tacggtattg atacgtcaga ctttactatt ccttatattg caaattggac taaaaataat
     4801 gaaaaaattg atccaaaaaa ctttagacag ataatgcaag aagttagaga aacgtctatt
     4861 gattttataa gtacgattga taagaaagtc ttggaagaac agcaaacagc atttaatgca
     4921 ctaaaaaatt cctttgttcc taatgagaat aaagagcaga tacaacaaaa atcagattta
     4981 gaatttcata atttctacga tagtacaatt aacgatccgg atagaacaat ccacggtagt
     5041 tatttagaag cgatggtgga ttattataga ggaaagtatg gagaaaattc tattgagctt
     5101 actcatagca attatgatgt tacagctaaa ataggagaaa actatgtgac atattattca
     5161 gatacgaaac aatttgatgt atgtgatgtt tatggtgtag tttttgagaa tccaagtgaa
     5221 aataaaaatg atcgtttatt attagatcga tcacctgaaa attgtgatta tgttgaaaag
     5281 tttgttgtag aaagtttgaa agtttgcgag aatcatgaac taaaaaatga tacgttaaaa
     5341 ttaagcgaac cgctaagatc agttgttata aatgaagcgg tttataatct atctgaaaat
     5401 gctaacaatt actataatga aaaatgttgg caagaagaaa ttgaaaacca aacaaatatt
     5461 aatgatgtaa aagaattaaa ctcttgtatg actgcggaag atttttacga atctcataaa
     5521 caagaaatcg aaaatacgat tacagatttt gaatctgtgt caggaacaaa tttaagagat
     5581 acaatagaca atgagtcata taaagaggtg gcttcagtgg tagcttataa attggttgca
     5641 actaaagtaa aatatgaaat ggatgaagga actttcaaga taccagaaaa ttctaacttg
     5701 gaaaagccga ctgtaaaaac agcagaacgt tcttttgaaa tggagtgaaa aaattatgag
     5761 taatgaacaa aattatgaaa caggcggaac ttatcgcctg tttcatggaa gtatgaataa
     5821 atttgatgaa tttagtttag atcataaagg tttaaacgga acagctaatg gatttggtgt
     5881 gtacttaaca cctaataaag aaatggctac aatgtacgct gaacagggta gtaacggaaa
     5941 taatggtttc gtatatgaag taaatgttga attagagaag ccgatttcta tgaatgaacg
     6001 cactattact gatgaagagc tttctaaagt aatagataaa ttacaagaaa gtaatgatat
     6061 tttaaatgat tacaatgatg ttagttatta cggagaaaaa gttgtaaaaa gagaagtgat
     6121 tgcttctctt agtgagaatg aaaatgacgt agatttgatt aacgacattg gaaatacaat
     6181 tggagatcat gaagctactg ctaaggcatt ttatgacgtt gggaaatata cacatattat
     6241 tgcggaagaa cagttaagac aaaaggatgt agttatcgta gtgttacagc ctgataaagt
     6301 aaagattgaa actattcacg atttaaacga ggaacggaat agcttagaaa gtagtgagga
     6361 catgacagat aaatttgttg ggcataaggt ttcggaagtt cccattcgag aagttgtgat
     6421 aaaagatttt gagcaagagc tagaaacaac taattattat gacaaacaag cttggcaaga
     6481 gcgcattgaa tacttagaaa acatggaaga accagtttcc agattagcat tagatagtga
     6541 tattaatgaa ttttatgata gtcataaaga agaaattgaa acgaaattga caaatttatc
     6601 cgaaaagtta gagatcgata aagaagaact gctaggtttt ccagtagata cttcttctac
     6661 ggaatacaaa aaagctacgt ctatcatggc ttacaacggt gcaatgagag aaatttataa
     6721 tgaaattgaa aatgatacgt ttgaaatacc acctgtttct gaagaaatgg agaataagat
     6781 tttgaaattg actaattttg aaaatacaac aaaatttgaa atggagtaga taaatgaatg
     6841 atagaacaag aaaatagaaa ttttttagaa acgcaaggtg aatatcgtca aattaataat
     6901 agtcattttg taatttgtga agatgtacaa atcgctttta aaatggcttt gaagtacgga
     6961 aaaagtatta gaaaaaagat aattagagaa aaaaagagca agaagatagg cttttgggag
     7021 ttatctctta gggatgggct aaactttatt ttctgtacag aggattatta tgaaaaatac
     7081 aagtgtacct tatcgtaatt ttactagtga tggtctagta aagggggttt ttgtttggat
     7141 ttgaaaaagg caaaaaaagt aagtatcgtt gatttttgca aacaaaatgg gataggttta
     7201 aagcaagatt caagggaata ctatagatta gaagatcatg attcttgtgt aatcagtgat
     7261 cgcaaaaacc tttttaaatg gaactcaaag gatttagggg gagatatttt tgattttatc
     7321 aaagcctact atgattgttc atttaaagaa gcaaaacaga aacttgaaga tagcgattat
     7381 gaagttcatt cgttcaatac aaaagtgaaa aaagctccgt accaatatca gaaagaaaat
     7441 gaagtgccaa tatctgttgc aaagtcttat ttagtaaatg agagaagaat cgatacagct
     7501 ttagtggaag attttttatc taaaggacta attaaacaag ataaaaaagg aaatgttttg
     7561 ttcttatgga aaaaagatca agaaatcgtt gggtgtacgg aacaaggaac tagaaaattt
     7621 tttcatgaag gcaaacaaaa ggatgttaca tggaaaaaaa tacaagaaaa ctctaaagag
     7681 tattcaggat ttaacgtaac ttttggtcaa ccagataaaa tgtatttttt tgaaagtgaa
     7741 atagatttga tgtcgtacat tacacaaaag ccagaaaaag ccgaaaatgc tacgtttgtt
     7801 agcatgaatg gattaaaggt aggtaatgta ttgcatgggg ttacagagca ttataataag
     7861 tttaaatcat tgccttcaga aacagtcctt tgtgttgata atgataaagc cggacgtact
     7921 tttattgaaa ctgaatttaa agaaaaagga attggaagag aagggatagg aatcagtgaa
     7981 gtaaaagtcg atgtaccttt gcgagaggga tttgattgga atgataattt aacaagagga
     8041 aaagaagctt ttaaacgctt caagttagaa gatgtatcaa aaatcgtgaa cttcacacat
     8101 aaattatctc ttaatactcc tgtatatgaa atggaataac aagaagaaag tagttgatgt
     8161 gttatgaacg atacaatagc aatcttatca gattcattaa ttgatttaga taagacgtct
     8221 acagactata tgattattga agaacaactt aaaaataaaa atccagagtt cgatattcaa
     8281 gaaaaatcat tgcgtattga attaccaaaa tttaaacaaa aaaaattaat aactattctc
     8341 tttgagatta ttgaacgata tgggcttact tattatcaaa tttggacgga taagaaagat
     8401 ggccaatata caaaaggggt aagcatagaa gccgttagag aagacgatca agtaagtatt
     8461 aaagcagttc aacccttaat ggggtataac gaagaattat tgaattgttt aaacgtctag
     8521 tgtaaactag gcgttttttt acattatttg gctacgaata gacagttaat taaactaaag
     8581 aaaaccattg actacaagta gtcattcctg tatgatatag ccatagacta caagtagtca
     8641 aagactcaaa cccttgattt aataaaaagg agagataata gatggagcag acaagaatgt
     8701 tttggtcaga tcgaatgcaa gatcacttgg attgtattta caaaaccaga gagcaattaa
     8761 ataagttaga tactgtcaga tattatttta cctacttcag tgagaatgac gaacaaagat
     8821 gggatatttt tcatgatggt aattacgaag aattttttta cactgaaagt taattatttg
     8881 ttgaaatgat gaaggattct atttgatgaa agggaacgga aaaccgttcc cctaaaaaat
     8941 atccaaatga ctacaagtag tcatggaagg agagcggaat aatggtaaat aaaaaagaaa
     9001 cgtttaaagt aggtatggaa gtacagggcg ttgttattgg gagcggagag cttatcgagg
     9061 gagaagtaat tagtttgtta gctaatgtag cggtagttaa agcaatcgga aaagcaccgg
     9121 tagtgatgaa aaaatgcagc atgatgtgtc ttgatggttt aaacaatcca ataactcaac
     9181 gctttgagaa cgtgatataa tagaaaaaag aagattttct actgggagag attattatga
     9241 aagactatgc ttcacgtaaa tttatccact tagagcctat gaaagaattg gttattaatg
     9301 ggaatgaata taatgattac aaatctttat ttgtaaagga acagcctaaa ggcggtattc
     9361 ggatgaaaaa tttaccggac ttccgattta tcgaagtaaa caatttagga attatagaag
     9421 actatttaga taagttagat gaagcgataa agcaatcaat tgaaaaaaat ggtgaagatc
     9481 atatacttac ggttgtttta aaagacttga aagatagact agaagctgaa gcagagtact
     9541 ttaataaaga ctaataagaa gggaatgaga gctatgacag aggctgtaaa agaattaaag
     9601 aaaatgtatc ctgatgtttt gaatatgact gtagatgatt ttcatgaagc cttaaaaaat
     9661 gcagaaagtg aggaagaaag gactttctac cttacacttt ctagttttgt gacacgagta
     9721 gatcaaaaaa aagtaattaa tcagaaagac tttaaaatat gagtttagaa aatgaaaaac
     9781 cgtattatgt agttgttggg ggcgttaacg gcgccggtaa gtctactatt tatcaagttc
     9841 aaaatttaaa aagaaaagaa cttgatactg atagaattaa tgcagatgaa attttgagaa
     9901 gttttggcgg aaattggaaa aaacaagcag acataaacaa aagtatgcgt gaagttctaa
     9961 gagagatcaa aggtcatttt aaagaaggaa aatcttttca ccatgaaacg acttttggtg
    10021 gtaatccata tcccgataaa atgagacttc taaaggctaa aaaattaggt tatgaatcgt
    10081 ttttaatcta tataggacta gattcaccag aaagagcgat tgaaagggtt aaaatccgtg
    10141 ttgctaaagg tggtcacggt gtgcccgaag atgtaataag gaaacgatac gaattttctt
    10201 taagaaactt agcagagtta gaaaaatatt ttgataatgt aagtatctat gataattcag
    10261 atgagttttc ttatatttat gttaaagaaa atcatacagt tgtagccaat aatacgcaag
    10321 aatataattg ggttcccaaa gctattcaag agaaaaacgc tgaaattcct ataagcaaac
    10381 aaattatcaa ggatatatcc caagaagggt tgagtccaaa agaaaccctt gaaagaatat
    10441 atttagcaag tgatgatttt attagtgatc gtttttttaa agcagagcaa gttgatgatt
    10501 tttatatgaa ccataaaaat gaaatcaagg aacggattct agcaattagt ttttctacag
    10561 gagtacctga agatgaatta gtaggccaag ataaaaaaag aaattcttgt aaattggcat
    10621 atattaatag tgtaggtgag cttttaggaa aaactgatag cgagctatca ggcatggaag
    10681 taaacagaga tagaagtata aaaaaaactg attttgaaat tgaataaaag aagatgtagc
    10741 tgaatcagtt aatagtaaat aaatttaata aggagtgcga aagcacttct tattttttaa
    10801 atagaatgac tacatgtagc cagaagggat aatatattat ttttagttct tgttttttaa
    10861 atgattacta caagtagaca attgattgtt gattatggtc taataaatat gttagtatta
    10921 ttatatacca gatacataat aatgaggtgc taaaattgac aataataact atgactaaaa
    10981 aaatagaagt atatgttgat ggatcacaag aggaaagaga ttcttttttt gggttttttt
    11041 ggaaaaatag tagagaatat gtaaaagtgc tgaatatggt atatagacag ttgatgtttg
    11101 aagagctgat aaaagatgca atcaaagaaa tggatcaagg ctatgttgag agagaaaaaa
    11161 gtattcaaaa taggattgaa aagttagcgt atactgatcc aatagatcaa aaaaaacttg
    11221 ataaagcaaa agctgaatta tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa
    11281 tattaacgca agcaatagga ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat
    11341 tagactttga ttttggagat tttgttgatt cagcaaatat gaaagctagt aaggattttg
    11401 gcaatgactt tgttggaatt gttactggtc aaagaacacc tagagtctat agaaataaaa
    11461 gtaaatatgg attgttaccg ttcagaggtc gggatattaa gattcataaa gctgacaagt
    11521 tttatatctc tttacctaaa ggatttaggt ttaaagtaaa tttaggtagc aaaccgagaa
    11581 aagcgagaga tagcgcttta actttggggt atattatgga cggtaaatac aagttaaatc
    11641 aaagtaacct aatttttgaa gggaataaat tgttttttaa tctaacaatt tcttttgaaa
    11701 aagattatca taagctagaa tttaaagaag gttctgagtt aaccataaag tttaccgatt
    11761 tgtgtagcgg tgaatgttta gttaataata aatctgtgat tgattttggt gacagcaaat
    11821 atataagtaa cttcaaaaat aaaattaaag caatgaaatc aagagagcag agccgaggag
    11881 ttttttctaa aggcggtcat ggtcgaaaaa gaaaattaga tcaagataga tgggaagcca
    11941 taaagaaaag agaaagtaat tttgttaaga cttataataa tcaatgttca gcaaaaattg
    12001 ttcaaacagc tattcgcaat aaggcacaaa taatttgtat ttctgaacca gataaaagtt
    12061 atggggaatg ggcttattat cagttgaaag aacaaataat aaataaagga aaaagatacg
    12121 gaatagaagt taaaagtgtg taaaataaaa aatcgttgat ttggcaacga aatagtgagg
    12181 gggcactcac tcactgaagg gtcaactccg tcatattgtg tgaccagacg gaaggacgaa
    12241 tgttgttgag gttttggagc tatataaaac gacatggttc tcaaatgata aagccattct
    12301 ttttcaatta atgattgttt tggagctata taaaacgaca tggttctcaa atgatacgaa
    12361 agaagagtat atccaaaaag ttgacaaggc tttggaatca tataaaatag catagtactc
    12421 aaaaatagat atagtaatat tttggaaata gttatagata agtagagaaa tcgttgattt
    12481 ggcaacgaaa tagtgaggga aactccactc actgaagggt caactccgtc atattgtgtg
    12541 accagacgga aggacgaata ttgttgaggt tttagaacca tgtaaaacaa caaagttctc
    12601 aaatagaata agttcaaaga aggtttagat gatggtttta gaaccatgta aaacaacaaa
    12661 gttctcaaat gtgtagataa ataattttta aatattaatg ggttagcata tcttttaggt
    12721 cttagttaca tgctaagact atttttttgg tttatatgtt acgtaagggt ataccctatt
    12781 gtaacactag tggaaatgcg atataatagg aattagaggt gtgacacaag ggtgtgacat
    12841 aaaggggtgg atatactgaa gacagaaaag aaaaaattgt agcaaccaaa aaagaaaaaa
    12901 tgagagttga taaactcacg aaaaaattcc tagtttgttt gactagagta aattaagtta
    12961 tttgggtgtg aaaatatgtt agtaggatat gcaagagtaa gttcagacga tcaaaaccta
    13021 gaacgacaac ttgaagaatt taatcgtgtg ggtgtcgaaa aagtatttgc tgaaaaaaaa
    13081 tcaggtcgga atacagccga acggccagag tttaataaga tgttagcgtt tgttagagaa
    13141 ggagatactc ttgtctttga atctcttgaa cgattaggaa gaaattctga tgagatattg
    13201 gatactgtat cgtttataga taaaaatggt atgagtctta tggttttgaa tttgccaatt
    13261 ctagatacaa agtttggtga tcctaactta gaaaagcttg tccgaagttt ggttattagt
    13321 attttttctt ggacggcaca aaatgaaaga gaagaaataa agagaaaaca aaaacaaggt
    13381 atagcaattg cgaaaaggaa gggatattac aaaggaaaac cttatgagta ttcagaaaat
    13441 gccaaaaatc cacaaaaaag atttatttat aacgagattg ttcgaatgtt aggtgaagga
    13501 ttaccaatca aaaaaattgc agaaaaaaca ggaacaaata gaaatttagt ttatagaata
    13561 aagaaaagaa taagtgaatc tggttgatct taagttcttt gttaaatcaa cgttacgaga
    13621 ggaggattct aagcctgttt caaactttta agatgtacaa ctatacaaag aagcttctaa
    13681 ataaaagagg ttatattggg gaatctctaa tatagatcat tgactggaag gatagtttat
    13741 atattcaaag aagggtgttg aaaaaaaaag gaagaaaaaa ctaataattt ggatttttaa
    13801 agataataca gtcaaaagga gtttttaaaa atgaatcaac ctagaagaaa aaggttagaa
    13861 agtatttctg aaaaaatttc cgagttgaaa gatttgcttg aggaaattaa agaggaagaa
    13921 gaagaataca aagagaatat gccagaaaac ttatgggggt cagaacgcta tgaaagagcc
    13981 gaggaagccg tagggcaatt agaagaagct gacgattatt tggatcaagt aattggatta
    14041 atcgaggaag taatcagcta aataccaagc tagcaaaaga aatataatca aaattaaata
    14101 aaggggaaat aacaatgact caaaactata ttgataactg gagattattt tctggaaaat
    14161 taatgacaaa agacagtatt gcagaaatgc cagaatcatt ggaaaaggaa catattctta
    14221 aagtccgaga aatgttgcac aaggaagggt ttaatgcaga tttattccga gttggggaac
    14281 ttccaattga aggagtttat tattttgagc gagctgtatc ggagaacgga gtcgattatt
    14341 tttcaataat tgttgaagac gatggttctc ttacgccaca gtatacaacg ttagaatgcg
    14401 acgaaagcgg atttaatact tttccttgca ataccattca agaatcaatt gataagcatg
    14461 agtgctaatt aattatctac aggaaggtgt tttttatgaa attagagata aaagaagttg
    14521 tctgcgattg gggaatctat gtagacggac aaacatatcc atttatgatt tttaatagta
    14581 aagcaaatgc acaagagata atgcgtatca tggagttgga taataagcac gaaagatttg
    14641 atgaagtaaa caaaatggag aacgaatgtg aagcagtgga ataccataaa acatgcgcca
    14701 ataataagta tattttaggt gcaatggggt taggtaaaac agcttctatt aaacaggtct
    14761 acgaagaatc acaggagaat tgcgtatatg aaaattcgaa ttaaagagtg agaatatgtg
    14821 ggagctaatt tatttagttt tata
//
LOCUS       DAXWLB010000062        14773 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXWLB010000062 DAXWLB010000000
VERSION     DAXWLB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.062"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            121..933
                     /locus_tag="ACGE4H_001470"
     CDS             121..933
                     /locus_tag="ACGE4H_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFF8364889.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLLRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            930..1730
                     /locus_tag="ACGE4H_001471"
     CDS             930..1730
                     /locus_tag="ACGE4H_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFF8364890.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE4H_001472"
     CDS             1891..3159
                     /gene="rseP"
                     /locus_tag="ACGE4H_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFF8364891.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3227..4936
                     /locus_tag="ACGE4H_001473"
     CDS             3227..4936
                     /locus_tag="ACGE4H_001473"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFF8364892.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5148..9500
                     /locus_tag="ACGE4H_001474"
     CDS             5148..9500
                     /locus_tag="ACGE4H_001474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFF8364893.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE4H_001475"
     CDS             9644..10117
                     /gene="rimP"
                     /locus_tag="ACGE4H_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFF8364894.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE4H_001476"
     CDS             10141..11316
                     /gene="nusA"
                     /locus_tag="ACGE4H_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFF8364895.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11338..11631
                     /locus_tag="ACGE4H_001477"
     CDS             11338..11631
                     /locus_tag="ACGE4H_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFF8364896.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11628..11939
                     /locus_tag="ACGE4H_001478"
     CDS             11628..11939
                     /locus_tag="ACGE4H_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFF8364897.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11952..14258
                     /gene="infB"
                     /locus_tag="ACGE4H_001479"
     CDS             11952..14258
                     /gene="infB"
                     /locus_tag="ACGE4H_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFF8364898.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE4H_001480"
     CDS             14285..14632
                     /gene="rbfA"
                     /locus_tag="ACGE4H_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFF8364899.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         5058 a         2595 c         3283 g         3837 t
ORIGIN      
        1 aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat aggaaaaaca
       61 gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg aggaaaaaga
      121 atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga atttcaattt
      181 aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa tggtcgttgg
      241 gcgcaaaacc gtcgtttgct acgagttgct ggccataaag aaggcatgga aacagtaaag
      301 aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta tgccttttcg
      361 actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact acctgtcgac
      421 ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca tgtcatgggt
      481 tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat tgagcaaaca
      541 aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagtag agcagagatc
      601 gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca cgcagaagaa
      661 atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa agagttacaa
      721 gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt tctgttatgg
      781 cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt tgatggtgcg
      841 catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg cggtgtaaaa
      901 aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtgatcaca gcagctgtgg
      961 cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag cttacagcag
     1021 caatcttagc ggtagtagga gtttacgagc tgttccggat gaaaggattg acactcttaa
     1081 gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca aaagaaagat
     1141 ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt atagttatga
     1201 ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa gcaggttttc
     1261 ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat gcacgaacag
     1321 aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat atcggagcat
     1381 atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg cccaacaaaa
     1441 cgattgaagg tgcgctcggt ggtatcggta gtgctgttgt agtcgcattt ctttatttat
     1501 tgctataccc agcaaaagaa ctattcaatc atggtatgtt tgtcatgctt ctgatgacga
     1561 ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc aaacgtcatt
     1621 atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta gaccgtttcg
     1681 acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag taagtatacc
     1741 tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta tattgatact
     1801 attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt ttataaaaaa
     1861 cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt tatcatcgtt
     1921 tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa acgatcagga
     1981 atcctcgtcc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca tcaagcaaaa
     2041 gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg aatggcaggc
     2101 aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt actaaattct
     2161 gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa tgctatcccg
     2221 atggaattga gccgttatga tttagaagat gagctgacta tcactggtta tgtgaatgga
     2281 gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat tgaaaacgac
     2341 ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa attgtggcag
     2401 cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat cgtgttattc
     2461 atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg cgtaggagag
     2521 atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga ggtagtcagt
     2581 gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac gaaaaatcca
     2641 ggtaaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt cgacgtgaca
     2701 ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa agccccaatg
     2761 aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag cggttcattg
     2821 gaaattttca aagccttagg atcactattt acaggtttca gtttagataa attaggcgga
     2881 cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac aactgttatt
     2941 agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc gattcctgcc
     3001 ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa accattaagt
     3061 caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt gttgatggtg
     3121 ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac aaatagggta
     3181 taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga gacaatcaaa
     3241 aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa gccatcaaat
     3301 cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat atctgccatt
     3361 agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg aaaaaatcgg
     3421 agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag aatccgggcg
     3481 ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc gaaaaatgat
     3541 ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa tcaattcata
     3601 taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg aaaaacgacc
     3661 tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcatatt ctttccatgc
     3721 aacggaagaa agtttggacg agacatataa agattacgaa aaagcctata ccgaagtatt
     3781 caagcgttgt ggcttagaat ttcgctcgat catcggagac ggcggcgcaa tgggcgggaa
     3841 agactcaaag gaattcatgg cgatctcaga aatcggggag gatacgatct gttattcgac
     3901 tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat caaaaaaatc
     3961 gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat caattgacga
     4021 agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat tgttcattgc
     4081 ggatgaacaa cctgtccttg ttttggtacg tggagaccat gaagtcaatg acgtaaaact
     4141 gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag cacaaaaata
     4201 tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg taaaagtata
     4261 tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg aaacaggatt
     4321 ccactataaa aatgtgaatc caaaacgcga tttcactgtg ctttcttatg aagatcttcg
     4381 ttttgtacaa gaaggagatc cttcaccaga caacaacggt gttcttgcct ttactcgagg
     4441 aatcgaaatc ggacatatat tcaaaatcgg cactcgttac agtgaatcta tgggagcaac
     4501 tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg gtatcggtgt
     4561 aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa tcaattggcc
     4621 aaaaggaatt gcgccatttg atctgcatat cgtccaaatg aatctaaaag acgaatacca
     4681 aacaagcctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg aagtactggt
     4741 tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga ttggctgtcc
     4801 aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga aaatcaagaa
     4861 aactggcgaa atggttgaag tacgtaaaga agaattggcg aatactttac caatcctctt
     4921 gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga atcagctgtt
     4981 tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg cagttccaat
     5041 tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa agaagaggat
     5101 aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg tcagaaaaac
     5161 gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa aaaaatcatc
     5221 cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg cgtttatggg
     5281 aatttacgct acatttttcc aaaattttgc ctattatgct atatcggtca ttgactcagc
     5341 atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta gcagatgacc
     5401 agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa aatcagcaat
     5461 gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata aaagatcgta
     5521 aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa caatacttgc
     5581 cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc gaaccagaag
     5641 tggatgagca gcaagcggaa cgtgtattga aactttttga agaacgcaaa caagaacagg
     5701 cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa aagaaaaagg
     5761 aaagaaaaga gcaatcgcca gctcttgaag gacccatcca gctaggacga aatattccag
     5821 ccgatgaacc aacgacacca atgatcagta tcgttgaaga agaacgaaga gtcacacttg
     5881 aggggtacgt attcgacaaa gaagtcagag aactacgttc aaaacgcaag atcttgactt
     5941 tgaaaatcac agattatact tcatcattta tcgtcaaaaa attctcgaac ggcgaaaaag
     6001 atgaacaagt atttgatgcg atcagtgtcg gtagctggtt aaaggtacga ggaagtattc
     6061 aagaagatac gtttgtccgt gatttggtca tgaatgcgca agatatcata gaagtcaaac
     6121 atacacctcg caaagattat gctcctgaag gggaaaaacg agtagagctt catgttcata
     6181 gcaatatgag tacgatggat gcaacaaaca gcatctctga tcttgtggca caagcaggaa
     6241 aatggggaca ccgagctatc gccattacag accatggtgg tgcgcaagcc tttccggaag
     6301 cacatagtgc tggaaaaaaa gcaggtgtga aaatcttata cggcgtggaa gcgaatgtcg
     6361 tagatgatgg tgtcccaatt gcttataatg atgcacatga agcattgagt gaagccacat
     6421 acgtcgtatt tgacgtggaa acgacaggat tgtctgcagt atatgacacg atcatcgagc
     6481 tagccgcagt caaaatgtat aaaggaaatg tgatcgaaag ctttgatgaa tttatcgatc
     6541 caggacatcc attgtcacga acaacggtag atctgactgg tatcacggat gaaatggttc
     6601 gagggtcaaa gtcggaagaa gaagtattgc ggatgttcct tgaattttca aaagatacaa
     6661 ttttggtagc ccacaacgcg gcttttgata tggggttctt gaatactagc tacgcaaggt
     6721 atggtattcc tgaagcggcc aatccagtca ttgatacgtt ggaattagct agatatctgt
     6781 atcctcaatt caaacgtttc ggattaggtg tgttatctaa aaagtttggt gttagcttgg
     6841 aacagcatca ccgagcaatc tatgatgctg aagcaactgg tcacttagca tggatctttg
     6901 tgaaagaggc aatggacaat cataacatgc tttatcatga tcagctaaat gagcatatcg
     6961 gagaaggaga ttcttacaaa agggctcgac ctttccatgt aacgatttta gcaaaaaatc
     7021 aggcaggact gaaagatttg ttcaaactga tttcaatgtc gaatgtcgaa tacttcgaac
     7081 gcgtcccacg aataccacgc tcccaattga aaaagatgcg tgaaaatcta ttgatcggct
     7141 cagcttgtga taagggagaa atattcgaag ccatgatgca aaaaggggta gaagaggcaa
     7201 gaaaccgagc aaaattctat gattatattg aagttatgcc aaaagcggtc tatgcgcctt
     7261 tgatcgagca agaattggta aaaaatgaac atgatttgga agaaattatc caaaatttgg
     7321 tagaaatcgg aaaaagtttg gataaaatcg ttgttgcaac aggtaatgta cattatctga
     7381 acgaagaaga tgccatttac cgaaaaattt tgatcaattc tatgggcgga gcgaatccgt
     7441 tgaatcgtca tagtttgccg gatgtccatt tccgaacaac cgatgagatg ctaactgcat
     7501 ttcacttttt aggggaagaa acagcgaaag agatcgtagt agaaaatacg aataaaatcg
     7561 cagatatctg cgaagaagtg attccagtca aagatgaact ttatacaccg aagattccag
     7621 gttcagaaga tgaaatatca gaattgagtt acacaaaagc aaaacaaatg tacggagatc
     7681 cgttacctga gatcattcaa aaacgattag aaaaagagtt gaattctatt aatggtaatg
     7741 gcttctcggt tatttatctg atcgcccaaa aactagtgca taaaagtaat gaagatggct
     7801 atctagtagg ttcacgtggc tctgttggct ctagctttgt tgcgacgatg acaggaatca
     7861 ctgaagttaa tccattagcc cctcattatt attgtccaga gtgccaatat tccgagtttt
     7921 ttgaagatgg tacgtatggt tctggatttg acatgccaga aaaacaatgt ccaaaatgtg
     7981 gtgcacgctt aaacaaagac ggtcatgata ttccatttga gacattttta ggattccatg
     8041 gagacaaagt gcccgatatt gatttgaact tctcaggtga ttatcaagca gaagcccata
     8101 actatacgaa agtattgttt ggcgaagact atgtttaccg tgccggaacg attggtacag
     8161 tcgccgacaa aacagcatat ggctatgtaa aaggatacga aagggacaat aatctgcagt
     8221 ttcgaagtgc agaagtcgac agattagcaa aaggcgctac tggagtcaaa cggacaaccg
     8281 gccagcatcc aggggggatc atcgttattc ccgattatat ggatgtctat gattttacgc
     8341 cgatccaata tccagcagac gatcaaaatt cagaatggaa aacgactcat tttgatttcc
     8401 actcgattca cgataatgta ttgaaattag atattctagg gcatgatgac ccaactgtta
     8461 ttcggatgct tcaagattta tcagggatcg acccacaaac gatccctacg gatgacccgg
     8521 aagtgatgcg gatatttgcc ggtcctgaag tacttggagt aagtcaggaa caaatttatt
     8581 ccaaaacagg gacattaggt attccagaat tcggcactcg ttttgttcga gggatgttag
     8641 aagaaacgca tcctacaact tttgcagaac tactgcaaat ttccggacta tctcatggta
     8701 cagacgtatg gttaggaaat gcggaagaat tgattcgacg aggagatgcg acactggcgg
     8761 aagtaatcgg ctgtcgtgat gatattatgg tttatttgat ccatgctgga ttagatagcg
     8821 ggatggcatt taaaatcatg gaaactgttc gtaaaggaca atggaacaag atccctgatg
     8881 aattgcgaga aacctatcta tcggcgatga aagaaaataa tgtgcccgac tggtatatcg
     8941 attcttgttc aaaaatcaaa tatatgtttc cgaaagccca tgctgcagct tacgtattga
     9001 tggctcttcg tgtcgcttat ttcaaggttt atttccctat tttgtattac tgtgcatact
     9061 tttctgtacg tgcagacgac tttgatctag tctccatgtg caaaggaaaa gatgctgtga
     9121 aacaagcaat gaaagaaatc acggacaagg gattagatgc ttccgtaaaa gaaaagaatc
     9181 aactgacagt gctggagtta gccaatgaaa tgctggaacg aggattcaaa tttggaatga
     9241 ttgatttgta taaatcggat gccgttaact ttgttattga aggtgatacg ttgatcgcac
     9301 catttagagc tgtcccaagt ttgggaacca acgtggcgaa acaaatcgtt gaagcaagaa
     9361 aagacggtcc atttttatcc aaagaagatc ttgccacccg cggtaaagtt tcaaaaacat
     9421 tgatcgaata tatgaacgac aatggcgtat tgaaagatct accagatgaa aatcaattat
     9481 cgttattcga tatgctataa gcatctgttt tcctgtctgc ttgtaatttt aagcagagta
     9541 tggtatagtt cttataggta atgatactaa ctgtgagtga gcggattttt cgctcactct
     9601 ttttaatgga attacgcact taatttttaa ggaggcgaaa cttttgagta gcgtcgttga
     9661 aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac ttgtcgaagt
     9721 cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca aagaaggcgg
     9781 tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc
     9841 agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg gagcagagcg
     9901 gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc atgtctcctt
     9961 ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat ttgatgctga
    10021 acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa
    10081 aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa tcagaaaaaa
    10141 atgagtaaag aaatgttaaa tgcgttagac gctttagaag ctgaaaaagg gatctcaaaa
    10201 gaaatcgtga tcgatgcttt agaagcagca ttggtttctg catacaaacg tcactatggc
    10261 caagcacaaa acgtcgaagt ggaatttgat cagaaaaaag gaaagatcca tgtctatgcg
    10321 gtaaaagaag taacggaaga agtgatggat tcacagttgg aagtctcatt gaaagatgca
    10381 cttttaatca atccagcata tgagatcggt gatacgatcc gttttgaagt aacaccaaaa
    10441 gattttggac ggatcgctgc gcaaacggct aaacaagtta ttttacagcg tgtacgtgaa
    10501 gcagaaagaa cgattattta taacgaattc agtgcttatg aaaaagacat catgcaagga
    10561 atcgtcgaac gccaggataa acgttatatt tatgtcaatc ttggtaaaat tgaggcagtt
    10621 ctttctaaac aagatcaaat gccaaatgaa ttttatcaac cacatgatcg aatcaaagtt
    10681 tatgtttccc gagtagaaaa tacgtctaaa ggtccgcaag tatttgttag ccgcagccat
    10741 ccagacctat tgcgtcgctt atttgaacaa gaagtaccag aagtctatga tggaatcgtt
    10801 gaaattgtca gcatcgcccg tgaagctggc gatcgttcaa aagtggcagt tcgctcaaat
    10861 gatccaaata tcgatgcagt cggtacatgt gtcgggccaa agggacaacg agttcaagca
    10921 atcgtcaatg aattaaaagg cgaaaacatg gatatcgtcg aatggaatga agatccagct
    10981 gtattcatcg caaatgcatt gaatcctgca caagtcatgg atgtgatctt cgatgagaca
    11041 aatccaaagg cctgcacagt tgtcgttcca gactaccaat tatcgctagc aatcggtaaa
    11101 cgcggacaaa atgcacgttt agctgctaaa ttgactaacc ataaaatcga tatcaaatca
    11161 gaatctgaca tgacggaatt ttatgaaaat caagaacaac aaaaagaaac agaagaactg
    11221 catgatgaag caatcgttca gtcagattta acagacgatg aatatgaaac aattgcattc
    11281 aacaatgaaa cggtcgaaga aaaaccagaa gcttaacttt agtaaggagg caaagagatg
    11341 aaacaaagga aaattccttt acgcaaatcc gtggtttcag gtgaaatgaa acctaaaaaa
    11401 gagatggtcc gaatcacccg ttcaaaagaa ggggaagtat cgatcgatcc aactggaaaa
    11461 atgcctggac gaggagcgta tgtctcactt gagccggaag aagtacaaca agcttgggat
    11521 aaaaaaattc tcgatcgagt actcgaaaca acattaacag atgagtttta tcaagaactt
    11581 cttgactatg tcactcacca aaaagcacgt aaagagttat ttggaaaatg aatacagtta
    11641 atcggcaaaa agccatgaat ctcatcggtc tagcgatgcg cgcgggaaaa ctaatcacgg
    11701 gtgaagaact gaccattgct gaaatccgca aacaaaaagc aaaattggtg tttgtggcaa
    11761 ccgatgcaag tgagaataca agaaaaaaga taaaagataa gagttcatat tatgaagttc
    11821 cttgctttga gctattttct gaagcagaaa tcacgcagat gatcggaaaa cctcgcaagg
    11881 tattcggaat aatggatgcc ggatttgcga agaagaccaa ggagctaatt gaaggttagg
    11941 aaggtgattg catgggcaac aaaagaatct atgaattagc aaaagaactc aaaaaatcta
    12001 gtaaagacgt cgtggaaaaa gcacaacaac ttggcatcga tgtgaaaaac catatgggtg
    12061 cgattactag cagtgatgag aagaaattgc aacaagcgtt caaaaaccca agcacgaaac
    12121 aacaagctgc acaacaagca agcagaaaac cagccaatca acagccgaac gatcaacgta
    12181 aaaaagaaac aacgaacaat caacaaaaaa ataaaaataa ccgcagttat caagatcgcg
    12241 gtcaagggag cggtcaagtg aatcaaggaa aaaaaccaac aacaaaccaa aataatacgg
    12301 gaaataccag cagccaaaat cgtcaaggaa cgagccaagg cggcaatcaa aaccgtacta
    12361 acaataacaa caaaccaaac aataacaatc gcggcaaatt taataataat aaccgtaatc
    12421 gttataataa aaaaggtaaa aaaggcaaac agcaacaatc aactaaacca gctgtaccac
    12481 cacgcaaatt ccgtgaattg ccagatgtat tagagtatac tgaaggtatg aacgtagcag
    12541 atattgcgaa aaaaattcat cgtgagccag cagaaatcat taaaaaatta tttatgcttg
    12601 gtgtcatggt caatcaaaac caagcactgg ataaagatac gattgaactg ttagcgacgg
    12661 actatggcat ggaaccacaa gaaaaagtcc aagttgacat tgctgatatc gataaattct
    12721 tcgaggcaga agaagtcgat ccagataaat tagtctcacg tccgccagta gtaacgatta
    12781 tgggacacgt agaccacggg aaaacaacct tattggatac attgcgccat tcacgtgtaa
    12841 caagcggcga agccggcgga atcacacagc atatcggtgc ctaccagatc gatattgatg
    12901 gtaaaccaat cactttcttg gatacaccag gacatgcggc ctttacaagt atgcgtgcac
    12961 gtggagcaag tatcacagat atcacgatct tagttgttgc tgcagatgat ggcgtcatgc
    13021 cacaaacagt tgaagcaatc aaccatgcca aagctgcagg cgttccaatc atcgtcgctg
    13081 taaataaaat tgataaacca ggtgcaaatc ctcagcatgt tatgcaagaa ttaagtgagt
    13141 atgaattaat tcctgaagca tggggtggag aaacgatttt tgttgagatc tcagcgaaat
    13201 tcggccaaaa catcgaagaa ttgctggaaa tgatcctgtt agtcgcagaa gtagaagatt
    13261 taaaagcaga tccaacacaa cgcgcaatcg gaacagtgat cgaagcacgt cttgataaag
    13321 gtaaaggtcc tgtgtcaacc ttacttgtcc aagaaggaac acttcacgtt ggtgacccaa
    13381 tcgttgtcgg taatacttac ggacgtgtcc gtgtaatgac aaacgatctt ggccgtcgcg
    13441 ataaagaagc aggacctgca acgccagtag aaatcactgg cttgaatgat gtacctcaag
    13501 caggtgaccg tttcgtagtc ttcgaagacg aaaagacagc acgtgcagcg ggtgaagaac
    13561 gcgcaaaacg tgcattattg gaaaaccgtg ctgccacaag ccgtgtgact ttggacaact
    13621 tatttgaaag cctgaaagaa ggcgaattga aagaagttaa cgtaatcatc aaagcagatg
    13681 tacaaggttc agcagaagca ctggctgcat cattgaagaa gattgatgtg gaaggtgtcc
    13741 gtgtaaaaat cgtccattca gctgtcggag caatcaatga aagtgatgta accttagcag
    13801 cagccagcaa cgctattatc atcggtttca acgttcgtcc tacaccgcag gcaaaagtgc
    13861 aagctgatac tgaagaagtc gatattcgtc ttcaccgtat catctataaa gccatcgaag
    13921 aaatcgaaac tgcgatgaaa gggatgctag accctgaatt tgaagaaaaa atcaccggtc
    13981 aaatgatcgt tcgtgaaacc ttcaaagttt ctaaagttgg tacgattgca ggtgcctatg
    14041 taacagaagg atatattcgt agagatagcg gtgtccgtgt aatccgtgac ggaatcgtaa
    14101 tctatgaagg ccaacttgca agtctgaaac gattcaaaga tgacgtgaaa gaagtcaaaa
    14161 tgggttatga atgtggtgcg atgattgaaa aattcaatga catcaaagta gatgacgtaa
    14221 ttgaaggatt tatcatggaa gaagtcaaaa atgactaaaa actgataaaa ggaggccaat
    14281 cattatggct aactaccgtg atcgccgagt cggtcaagaa atcttaaaag aagtcaatga
    14341 tattttgcgc aaaaaggtcc gtgaccctcg tgtggagaac gtaacgatca cagatgtgaa
    14401 agttacgggg gacttacagc aagcaacgat ctattacagc attttatctg acttagcatc
    14461 agataagaaa aaagcacaag atggattgaa taaagcatca ggattgattc gtcgagagtt
    14521 agggcacaat ctaagtattt ataagacacc tgaattgttc tttgaacttg atgaatcagt
    14581 tgtctatggg aaccatatcg atgaattgct tcgcgacttg aacaaagact aatcaaaaaa
    14641 tcccgcaaat cctgtgaaat caggggttgc gggatttttt tgttttcatt aagacgtatt
    14701 acctaagagt tccatgactt tagtctggta gcgtgtcagc taaccaaaaa agagaaaaag
    14761 aaaagtaaaa aat
//
LOCUS       DAXWLB010000063        14118 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXWLB010000063 DAXWLB010000000
VERSION     DAXWLB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.063"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..556)
                     /locus_tag="ACGE4H_001481"
     CDS             complement(<1..556)
                     /locus_tag="ACGE4H_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFF8364900.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="ACGE4H_001482"
     CDS             complement(572..1051)
                     /locus_tag="ACGE4H_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFF8364901.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="ACGE4H_001483"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="ACGE4H_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="ACGE4H_001484"
     CDS             complement(2098..3204)
                     /locus_tag="ACGE4H_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8364902.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACGE4H_001485"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="ACGE4H_001485"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFF8364903.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACGE4H_001486"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="ACGE4H_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFF8364904.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACGE4H_001487"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="ACGE4H_001487"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFF8364905.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="ACGE4H_001488"
     CDS             8175..8873
                     /locus_tag="ACGE4H_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8364906.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="ACGE4H_001489"
     CDS             complement(9041..11680)
                     /locus_tag="ACGE4H_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8364907.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="ACGE4H_001490"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="ACGE4H_001490"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFF8364908.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="ACGE4H_001491"
     CDS             13523..>14118
                     /locus_tag="ACGE4H_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364909.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DAXWLB010000064        13956 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXWLB010000064 DAXWLB010000000
VERSION     DAXWLB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.064"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(182..1201)
                     /locus_tag="ACGE4H_001492"
     CDS             complement(182..1201)
                     /locus_tag="ACGE4H_001492"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFF8364910.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1834..2838)
                     /locus_tag="ACGE4H_001493"
     CDS             complement(1834..2838)
                     /locus_tag="ACGE4H_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8364911.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2851..3900)
                     /locus_tag="ACGE4H_001494"
     CDS             complement(2851..3900)
                     /locus_tag="ACGE4H_001494"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8364912.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3917..4738)
                     /locus_tag="ACGE4H_001495"
     CDS             complement(3917..4738)
                     /locus_tag="ACGE4H_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8364913.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4725..5504)
                     /locus_tag="ACGE4H_001496"
     CDS             complement(4725..5504)
                     /locus_tag="ACGE4H_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8364914.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5521..5991)
                     /locus_tag="ACGE4H_001497"
     CDS             complement(5521..5991)
                     /locus_tag="ACGE4H_001497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8364915.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(6003..6422)
                     /locus_tag="ACGE4H_001498"
     CDS             complement(6003..6422)
                     /locus_tag="ACGE4H_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8364916.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6503..9280)
                     /locus_tag="ACGE4H_001499"
     CDS             complement(6503..9280)
                     /locus_tag="ACGE4H_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8364917.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9471..9695
                     /locus_tag="ACGE4H_001500"
     CDS             9471..9695
                     /locus_tag="ACGE4H_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364918.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10474..12189
                     /locus_tag="ACGE4H_001501"
     CDS             10474..12189
                     /locus_tag="ACGE4H_001501"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364919.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12192..13955
                     /locus_tag="ACGE4H_001502"
     CDS             12192..13955
                     /locus_tag="ACGE4H_001502"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364920.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4113 a         2855 c         2414 g         4574 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacgac gattaactat tgatcatcta
      181 tttaacgaag agaaaattca ttctccattt ttccgtcacg taacatgatt tcttttgctg
      241 cttttttgat atcttctcca ttatacaaaa cattatagat tgtttgagta attggcatct
      301 caacacctag ctgttgagct agctctactg ctgcttttgt cgttgccaca ccctcaacga
      361 ccattcccat gttttctaga acttcttcca gcttatgtcc ctgtcctaac aagttaccgg
      421 cacgccaatt gcgagaatga acacttgtac aagtaacgat caagtccccc acaccactca
      481 aaccgataaa tgtcagagga ttggcaccca tcgcgacacc aagacggctg atttctgcca
      541 atcctcgagt catgattgct gcttttgcat catctccgaa tcctaatcca tggatagcgc
      601 cggcaccaat tgcaatgata tttttcagtg ctgcaccagt ttctacgcca atcacatcat
      661 tatttgtata aatcctgaaa taatcattca tgaacagttt ttgtacatag gctgctgctt
      721 tttgatcaat acttgcagct gtaattgttg tgatatcatg tactgccact tcctcagcat
      781 ggctaggacc agataataca acaattgcct gtcttttttc ttctggaatt tcttcagcca
      841 aaacttctga gattcgttta tgcgtatctt gttctagtcc tttacttgca tgtataatga
      901 ttggcttagt attcatttta ccaataagtt cctgtgcgac tgaacgaatc gcttttgttg
      961 gaacaacaaa taaaatagca tctgcgttat ccactgcttc tgccaattct ttatatccga
     1021 taatcgattc tgggatcgtc aaatcaggta aaaaatgctg atttgtatga tacttattga
     1081 tttcatcaat ctgttcaggt acatttcccc aaatacggac atcatgcccg ttttctgcca
     1141 atgtttgtgc taaagcagtg ccccaagatc caggaccaag aactgcgatt ttttgtttca
     1201 tggatattcc tccaaataaa taattgataa tttccattat agtatacatt tcttaaagtt
     1261 gggactaatt tccattgttt ttaagtgttt gtttacttct tttatcgttt ttacttgaaa
     1321 taatcaatga tatgttacaa tagtctacat tttattttta cactcgttat gctttgcttg
     1381 ttcctaagag gaagcagttt tgacaatgtt actaccagca ttactattag gaggttagga
     1441 aacttctcct agtgagtatt gatttaaaat acataagaga cagattgaac atttctaaat
     1501 ctgtcaggtc ctgcaacgga cagattgtct ctgttgacgg ttgacatagg aacttttgtt
     1561 ttagtcaaca ttctggatac aaaacgctat gtaaactggc gcaactcgtt gtacttggac
     1621 ttttactacg gatccatgca acaactaaat agtactattg cataacaaaa aatggttggc
     1681 acccacatgt cccattaaac agattataag ttgaactgta gataatgcaa aaaatgtgtt
     1741 ttataacgta attgatgcca atattttaaa aaaataaagg gagcagttat cgctgctctc
     1801 tttatttttt atattctttt tacccagtct atcctaataa tcaaactgac gatagtagcg
     1861 gcgtatctct aatggatggc agttcaactg ctccacatga gcatcgatgc gttgtgtcac
     1921 tacatgcatc aataagaaag acaaaacacg tccgcgatat ttttcactga taccttctac
     1981 tggatagtct tttgtgtcta tcaatgtata attgctgcaa atttttggca aaagtttagc
     2041 cacacgttca gataacactc tctgttcatc ttcgcctaaa aataatgtga ctggggttgt
     2101 ttcttctacg atctctaacg tcccgtgaag aaactcggca gcatggatag atttcgatgg
     2161 aagccagctt tgttcttccc agtaacacat ggcatatgaa taaacagctc cccattgatt
     2221 ccctgctcca ataaaataat gcatactatc acgtcgatgt ttttctgcaa atgataagcc
     2281 aaatgcatct gcttttttct ctgcttccac taaccctgtt ggtaaataac gttcgagctg
     2341 ttcatagtat tctgaataag cttcgaattc tccattcaaa tacatcaatc gatctgctgc
     2401 cataaaaaat ttgatttgtt ctgttccagg agctggataa gtgaccacaa aatcgcatag
     2461 ctgagacaac ttactgttcg ctttatcgat aaatccaatc agtgtcgctc ctactttctt
     2521 gatttgttct actgctttca ctacttcttg agttgtaccg gtaacagagg acaaaataac
     2581 gatagaatct tttgttaatc ttttgtttcc agtagtgtaa tattctgcag catgctgtac
     2641 aaagacaggc aagttgcttt tcccattcat ataagtgaca gcttgcatcg aggaagcata
     2701 cgttccaccg attcctagat aatagacagc gtcaaatttt tccgcataaa gtttatcgat
     2761 gatttcttct acttgcggac gtaaagctag tgcgcctttt attgctttga tttgttcttc
     2821 ttcattgaat ttcagcatga tttccacctc ttataattta cttttcaaga ctgtatcaaa
     2881 gtcttcaaaa atctttttgt tcaagtcaat acctttagct actgcggttt tataagcgaa
     2941 tagttgaaac ggaacgacaa gcgctaatac agacagatat tcatcacatt ctattgctaa
     3001 tccaagtgtt ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct ctccaacgta
     3061 ccgactcata tatctttgca acttttgcga acgttcctta tttacacttt ccccttcaat
     3121 aaagaatagc aaatgcgaag catctgcctc caggtatggg ccgtgcatat aagcttctaa
     3181 ctcaaatcct tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg cttctttagc
     3241 tgttccccag tttggaccat atccaatggt gacgaaacgc tgtgctaaac gaaataaact
     3301 ttgatatcca tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat ggttgattat
     3361 tttgcgcaac tgttgcatgt agtcttgttc tatttttttg ctgattttat ttttacttat
     3421 tccaattcct atccctaaga gtagtaactg taaaacagtc gcagaatagc cttttgtaac
     3481 gaagcctact gattctaccc cggcacctaa atcaataatt tgtgcagctt ctaaagcaag
     3541 tggactttgc acgtcatttg tgatagcaac aacaggtaac ccttgttttt gtatcatttt
     3601 tactgcttca acagtagaag cacttttccc gctttgtgac acagcaatca cagtatcgat
     3661 tgaaggagaa agttttccat aattgaaaaa ataaaaaggt tcttgaatat cgacagtaag
     3721 atcaccataa gattccaatg ctggcttcgc tgctaaacaa gcgttataag atgaacccgt
     3781 tgctaagatc agtaaattca ctgttttttt catatgttct cttgtcgaaa aatcattctt
     3841 tttcaagatc gtacttagta cttccttttc ttcatagata taatcttgca tcgtaaacat
     3901 tttctctgct ccttttttaa aaaatcccta tccacgaacc aaaaattcca aagaaagcta
     3961 gtccgatcaa gatcgttaat ggtttcactt ctttcttcaa tagataaaac actatgccaa
     4021 atgctccaag acataagata tttggaacga tatcatcaaa aatactttga acagtcacag
     4081 cattttctcc tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg gcagtcattg
     4141 ctccaacgac catcagtcca ataatagacg cgcctaatgt caaactttcc atcataccgt
     4201 ttgcctggat ctttttcaaa aaacccgttc ctaagcgata tccccaacct gttgctagat
     4261 agcgaaacag aatgtgcgga atattgaaaa tcaaaatgaa caagataggg cctaaaatat
     4321 ttccttgcat ggctaaagag gtacccacac ctgtggcaat caatcgcaaa gttccccaaa
     4381 agaaagaatc tcctaaccct gcaagaggtc ccatcaaaga tgttttgata ctatcgatcg
     4441 cagtcacatc aaattcagga tcatccttat tttcttcctc cattgcagcc gtgattccta
     4501 aaatcagtgt aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt ttcaaagctg
     4561 ctgccatttc ttctttcgac tgatataact ttttcaaaat cggaatcatt gcataaacat
     4621 aagccatatt catctgacgt tcataattcc aagaaaactc catttgaaaa gaacgccaaa
     4681 atacgtgatt tagctctttt ttcgttattt tgctgttttt gatctcagaa ttcttcatct
     4741 tcttctacct cccctgcagt tgtttgaagt ggtagttgac ggacattcat aatattgact
     4801 accacaactg ctaaaatcgc accaaaaata gctactccgg tcacaggaat ctctaagtaa
     4861 gaagctatag caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat cagcaaacga
     4921 gcaagcatcg caaaaccaag agctggaatc aaccctgtag ctactgacaa ccccttttga
     4981 acgaagtcag gaatcatatt taatagattt ccgatcgtat tgcttcccac catataagaa
     5041 accattacca caacaccaag catcaaggaa agaccgatac cggcaagcaa gtgcatatgc
     5101 tcgactcctt tataatttcc ttcttctgca taattgtctg ctttatggtt cataattgga
     5161 atcaaaagcc ctaagtaaat atttttcaat atcaatgtca acgtagcaat cggcaaacct
     5221 agtaaaagcg cggtttctgt accggctcca gctgtaatag caaaagccgt ccctaaaatc
     5281 ccaccggtca caacatcagg tggaatcgaa gcacctactg agaacgatcc gataaatgct
     5341 aactctaaag ttgcgcccat aatgatccca gtttttacat cacctaacac gattcctgta
     5401 aacagccccg tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata ttctgattgt
     5461 gcgacaaatg caacaattcc taataagact gcttgtacaa tcattgaggt tcttcctttc
     5521 ttacaaaact tctcttacat ccactttttt atctgctggt acttgacgga tttctacttc
     5581 tatttgtttg tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa tatttaccgc
     5641 ttttccgatg ctgcgagtac caggttttgc cttgatccct cctaagttga tggttttgat
     5701 ttctggatac gcttctgcca gcttatatgc atcctcgatc gattccacta cgatgaataa
     5761 cttatattta tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa ttgatttgat
     5821 cactagtttg actccttgtg gcttggctaa tttgatcgtt gtctttcgta tctcattcgt
     5881 aggtacatca tcattagcaa tcaaaataca gtctgcgcct attgtttgtg tccatgaaaa
     5941 agctacttgt ccgtgtaata accgatggtc gactcttgtc aatacaatca tttttcttcc
     6001 tcctaaaatt cgtcttcttc tattttctga ttgagccgct cgttacaaaa ctggatcatt
     6061 ttttttgaat cagttagcac ctgctggatc aaaacagttg tctctacttc agcgtccaac
     6121 cgagatacta gctcaatcaa tagtggcaaa ttcaatccgg caacgagata gacatgatcc
     6181 cgattgatat attgtaagaa ttcgttgttg acacttccgc caaaaagatc cgtcagaaca
     6241 atgatttctt tatctttgta cctcataaaa agctgcctta ctgtttccgc taaatcaaaa
     6301 ttctcatcag tatagcaatc gaatacagta atagaatgat ttttcccata gatcagctct
     6361 aatgaatcta atatccctga agcaaatttt ccatgggaag ctaagataat ttctctatcc
     6421 actttttcat ctcctctttt cacttacaca tactaaaaaa gcaagttgca tgccaaaatt
     6481 agtggcaaca acttgcttat gttcaaaatt cttcttctat cgagagaaac tctgtatctt
     6541 cactcaaaat atcatagata taggcaatct cagaattggg gatcatgaca ctataatgct
     6601 cttcaatgac actaaacgca gatttaatag cagataaccc ttctttttga cactgcttcc
     6661 atttctctaa tccttgatag gtttcgattg gcatttgtcg gatcaatcgt tcaatgagac
     6721 agctcacatg gacatacaac gctaattttt tgggattgct gatgcttcga ttcaactgca
     6781 cctctaattc tctcataaaa tgatcgattt ctcccatcac cttctctgta tctagaatag
     6841 tcacggaatc cagtactttt tccaatgaaa aattacgaat gatgcgctga ttgaatattt
     6901 ccttctctga agcattcata gtagtgtcca accattcagc tagtacagac atcttttccc
     6961 ccgagatgag gtcttctaat gaaagatagt cgatattggt gatatcagga tctgtggtgc
     7021 caatcattcc tacgacttcg tatatggaaa ataatgggtc actgtttttc ttatctttta
     7081 attgctgata ttcatatggg atgattttca actgacaagc cgtaggtaag cttttttcta
     7141 gcagttcact taagtggacc gccgtaccga ttccagtgaa gcaagtagta atgagtgctt
     7201 tgggtttgtt ttcctctgga tagagcagtc gccattctgg ctggatctgt cgaacagtct
     7261 cttctgctat ctcttctaat gatacatttt tttgcaggca ttcccctact gtgattgcta
     7321 aaggtgtcgt cacattattc aaaatcaaaa gcggtacgga taactgtttt ttaaaaaact
     7381 gatggatttc tttcagagat cccatgtcta ctaatatgat caatccattt gaaacatcgc
     7441 tgttttcgca ataatcaagg atttctgaag caatctgctg tggtgtgaca tctagtggca
     7501 tatcaaatga ttcaaataga tcctcttgta ataaacgatt tactacattt gccatactgc
     7561 tagcagttgc ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt tcttttgacc
     7621 atttcgcttt ttttagatag atcgtcagta aaatccgatc catacttgtg tcttcaatat
     7681 ctaatttgat cttacttaat tccagaattc gttggacata gtgataacta gatggataag
     7741 actgagaaac ttgttgttct aattctttga ttagttggat aatatcatgt tcttccggta
     7801 accatttgat agtgcttcgc tgatagagat agtaactcaa ggcatagaca ctgtttccat
     7861 cgaatttgat ttgataggca gtttccattt gtcgaaaggt ctctcggata gattgtgtcc
     7921 aataaaccag cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa aaatcaaata
     7981 attgaatcac ttcttgcttt aattttcctt cgcaagaata tagcagacag ttatttttct
     8041 tgaaatcaat taggatttgc tcaaaacttt taacgattcg ctgctgttcg ggctgttgct
     8101 tctccattaa ttgctctaag gtctggtctt gcgtaatata gattccttgc tgtgctgtat
     8161 ttttagtaat aggctgcgca agtagttctt tttgcaaatg atggatcgtg acatggattt
     8221 caggctgttc acgctgttca gcatatgctt ttgctgctga aactttgacg atatttttca
     8281 gctctcctat gtttccggat aatttctgac ttgttaaaag tgacaaagct ggtccactga
     8341 ctgacaaagg gctccccatt tttctttgtt cttcgagaaa aaaagagtaa atcagttcta
     8401 gccgttcatt acgactacgc tgattaagtg caggtagttc gattcggata ggtattcttc
     8461 ggatgaaagt tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa aagagacgtg
     8521 ttttgatccg gcgtgacaaa ttaggttctc ccatacgata aatcacacct tgatcaagat
     8581 aggtgaacag tttttcctga ccttctggtg ttaagcgatg tacctcgtct aaaaacaaga
     8641 tcccaccatc tgctgcttca aaagcccctt ttttattttc ttctgcacca gtgtacgctc
     8701 ccttaacatg tccaaacaaa ttgctagtca aaagttcagg attatctgca tattgtgcac
     8761 aattaaaagt gatgaatggt gcagaatctt ctagcagatc atgaagcaga caatactgat
     8821 agaccaaatt aactaaataa cttttacctg tgccactttc gcctgtaatc aaaagtggta
     8881 atccgccatc tggatagtaa agagctgttt tgatttgttc gatactttct ttcaaactgg
     8941 cgtcgtatcc gatcataaaa gagaataagt cttttggttg cgcgaaaaaa ggctgttcct
     9001 ctttgagatc atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat tgtttttcaa
     9061 aagcttcttt gtggaaataa atcactggtc gagaattgat tttgactagt agttgctcat
     9121 catttaactg gttcaaataa tgactgactg tgtttctttt taccccaaaa tgatcagcta
     9181 acctttgtgc tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct gtctggttct
     9241 ctaagtaaca taaaatttcc ttcttcaaca acttattcac ccctcctcca tcatagaaaa
     9301 cggattcatt ttgtcaataa aatctttaat aaagaattga aaaatatatc ttattataga
     9361 cagcagccta cttatccctt ataattttta ttctaaaaaa cgtacagttc cttccagttt
     9421 ttatactgga atttcaacat atgaaagaat aaggaggcta tgactattca atgaaatcgg
     9481 ataaatataa aaacaggaaa catacttcct ggaaaacttt tcgagaaatc atcggatctt
     9541 atctcgctgt agttgtattt ttgcctttgc tttatatggc tatgacagaa caaactcgtt
     9601 ttattggcgt agatacgcaa tatatagccg aaacaggatt tttattcatc tttatcttga
     9661 tttttgcgtt ctataaatcc ttccatacaa aataagattc acagatttca actttcctgt
     9721 ctgtttttag ctattacaaa tccttatatg tggtataaca acaatttatt ggcataatga
     9781 ggaaaaacga gttatgatgc tttcagagag taaagaggta ctgttgtcac catccttttt
     9841 attgattacg tacaccactc ttagtttttt cccattgatt acgaaaacaa ggtagaaaca
     9901 ttgatgttcc taccttgttt tcttttttta ttttcagatc aaaatctcaa gtagcttttg
     9961 acttgatgtc gggaggttgt cttcgttcca tcaaacctga tagaatctgc gccaaaccat
    10021 agttttctcc actattattt attcctctaa gatagtaccc acaataaacg aataaatatt
    10081 tttctcattt tcatactcct tctttgaaaa tcaattagat ttctctcaac ccactttttt
    10141 acaaaaatta cgtagcaaaa aagattgcaa gaacaaatta aaaaagaagt aaatagagtt
    10201 tcatctataa acatttactt gatatatttt ttatgcagaa ttcatttata gaaaaattaa
    10261 gtaaaataga cgttcctatg aatgaagaga ctggcatgtt ttctcatttt ctttttttta
    10321 aaaaaaatag tctattcaat ttctaaagct tcttcataat aaaactttca tttatgaaaa
    10381 ggcttgttct cttttcattc taaatttcct aagctacaat gtggtagtac ttgttttttc
    10441 aattgtggac aaataaataa taggaggaat catatgattc agttattacg ttatgcaaaa
    10501 gactatcgta agcaaatcat tttgggcccg ttcttcaaat ttcttgaagc tgtcttcgaa
    10561 cttgtcctgc ctttaatgat ggcttccttg atcgataatg gactaaaaat gaatgaccga
    10621 ggaaaaatca ttgaaatggg cttatggatg gtcgctatgt ctgtgattgg tctgatttgt
    10681 gctatcattt gccaatacta tgcttcagta gcttcacaag gatttggtac agagttacgt
    10741 aatcagctaa tcaaaaaaat caatactttc tctcacaacg agttgaatca ttttggaaca
    10801 gataccttga tcacccgaat gacaaacgac atcaatcagc ttcagctagc tttagcaatg
    10861 gtcattcgtt tattgatcag agcaccgttt ttaagtatcg ggtcagtggt catggctttt
    10921 gtgatcgact gggaagtagg tctgtttttc ttagcattat tgcctatttt ctcaatcatt
    10981 ttatttttta tcatcaaaaa aaccgttcct ctctaccaaa aagtgcaaga aaaactagat
    11041 cagctcaatg aacacgttag tcagaatctt agcggtgttc gtgtcatccg cgcatttgcg
    11101 cagacaaaaa aagaaacaga gaagttcgat aaaagcacaa atgatctagc gaacaattat
    11161 ttacgtgttt ccaatatctc tgccctatta tcacctgcta caaccttgat catgaatgtc
    11221 gggatcatct gtttgctgac agttggcggg atcaaagtaa atattggtag cttgcaacaa
    11281 ggacaagttc ttgctttgat caattatatg aatcaaatgc ttttggcact gatcgttgtt
    11341 tcaaacctcg tcgtgatctt cacacgtgcc gaagcttcag gaaatcgggt gaaagaggtg
    11401 ctagacacag aaaattcgat tcttgaagct gaggtcagta ctactcccga tttttcttct
    11461 gaagcaatta tcgcttttga ccatgttgac ttccgttata ctcctgattc tggtctttct
    11521 ctccaaaata ttacatttca gctgaagcga aatacagtcc ttggaatcat tggtccaacc
    11581 ggaagcggga aaacgacttt gacacaattg attccgcgtt tctatgatgt cagttccggt
    11641 acagttataa tggacggaat ggatgttcgt tcttggcagc tggatcatct ccgtcagcaa
    11701 attgctcttg ttccccaaac ttctgtacta tttaccggga ctattcgtga aaaccttcaa
    11761 tggggtaaga aagacgccac agaaaaagaa tgttgggaag ctctgaaaat cgctcaggca
    11821 gaagaatttg tccgtcagct tccaaatggt ttagatacaa aagtcatgga aaatgggaaa
    11881 aatttctctg gcggacaaaa acagcgactg acgattgcaa gagctttgat tgccaagcca
    11941 gcattactga ttttagatga ctctttaagt gcgttggatt atcaaacaga cttggatttg
    12001 cgtaaggcgc tacagactta tcttggcagt acagtgatta ttatctccca acgtgtccgc
    12061 tctattcaag aagcacatca tatcttagtg atggatcaag gtaagattgc tgctcagggt
    12121 acccatgaag aattacttgc tcaatcagca gaatatcagg aaatcgtcgc atcacaggag
    12181 gaagaatagt tatgaaagta aaaaaattat ccttgcagac gctccgccga ttcattcctt
    12241 accttactgc ctatgcaaaa gacatttggc tagctgtgat actaggaatc atcaatggga
    12301 ttgcaacagt tgtgatgacg ttacaaattg gtcaaagtat cgatcaaatg attggagaag
    12361 gacaggtccg cttcaatcga ctgttccatt tacttattct attcggtggt atcgttttaa
    12421 tcaacgtagt cagtcaatgg ctcatccaag tgttaagcaa tcggttagct tacttatccg
    12481 ttgcaaaatt aagaaaagat gcatttgctc atctgaatcg attgccactt cgttattatg
    12541 atcaaaactc ccacggaaat atcgtcagtc gatttacaaa tgatatggac aatatttcca
    12601 ttgcaatttc tgcggccttc aatcaattat tttctggagt ggcaatcatc attctttcgt
    12661 ttatttttat gatgcgtcta agtccacttc tgacattggt cgttctttgt tctacaccaa
    12721 ttattttcct tgtgagttgg atcgttgctc gtgcttcaca aacaaatttc gatagacagc
    12781 aaaggattat tggggatatc tcgggctttg tgaccgaacg aatcggtaac cagaaaatcg
    12841 tgaaagcttt tcagcaagaa gaagctagtc aacagcaatt tgaactgcta aatgctgatc
    12901 tatatgaaaa aggacaacgg gcacaatttt cttcttcctt gacaaacccg tcttctcgtt
    12961 ttgtagatca ccttgcttat ttatcgattg gtttagttgg cggcctattg gcattgaaga
    13021 gtggttcgtc aatcactgtt ggtgtgattt ccagctttac gatctactca agtcagtttt
    13081 ctaaaccatt tatcgaactt tctggaatca cgacacaact gcaaaccgct ttttctggtc
    13141 tagaacgagc atttgatttg atcgatcagc cagaagaaaa gcctgatttg ccagatgcct
    13201 atgccttgga acccaaaaat atcaaaggaa aagtctcttt tgatcatgtt tctttttctt
    13261 atgaccccaa gcagcgtttg atcgaagatt tcaattttac tgcttatcca ggtcaaacca
    13321 ttgctatagt aggaaaaaca ggggccggaa aatccacact tgttaatttg ctgatgcgtt
    13381 tttatgacac aacaaaagga acaattacag tcgatgacca tgacatccgc tctatccaac
    13441 gtgatacatt acgtaaaagt tttggaatgg tgttacaaga tacttggcta tttgacagta
    13501 cattgcgaga aaatcttcgt tttgggaatc cggaagcaag tgacgcagaa atcaatgatg
    13561 ccctaaaagc ctcttatatg tatgaatttg tagaacgttt gccggaaaaa ttagatacta
    13621 agattggcgg tcaagggtta aagatatcag atggacaacg ccaactgttg acgattgctc
    13681 gaacgatgat cagtaatccc cctatgttga tcttagatga agctaccagc tcagtcgata
    13741 cattgacaga gaaaaaaatc caacaagcct tcttagcaat gatggaagga aaaaccagtt
    13801 ttgtgattgc ccatcgcctg tccacaatca aaaatgcgga ccagatatta gtgatggatc
    13861 agggagctat tgtcgaaatg ggcacccatg atgagctgct tcaaaaagat ggttattacc
    13921 gacggctcta tcaagcacaa ttcacagcac aataaa
//
LOCUS       DAXWLB010000065        13688 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXWLB010000065 DAXWLB010000000
VERSION     DAXWLB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.065"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACGE4H_001503"
     CDS             complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACGE4H_001503"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFF8364921.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACGE4H_001504"
     CDS             complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACGE4H_001504"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFF8364922.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8110..9078
                     /locus_tag="ACGE4H_001505"
     CDS             8110..9078
                     /locus_tag="ACGE4H_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8364923.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9096..10001
                     /locus_tag="ACGE4H_001506"
     CDS             9096..10001
                     /locus_tag="ACGE4H_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8364924.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9998..10804
                     /locus_tag="ACGE4H_001507"
     CDS             9998..10804
                     /locus_tag="ACGE4H_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364925.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10961..11929
                     /locus_tag="ACGE4H_001508"
     CDS             10961..11929
                     /locus_tag="ACGE4H_001508"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFF8364926.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12246..12770)
                     /locus_tag="ACGE4H_001509"
     CDS             complement(12246..12770)
                     /locus_tag="ACGE4H_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFF8364927.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13057..>13688
                     /gene="scm"
                     /locus_tag="ACGE4H_001510"
     CDS             13057..>13688
                     /gene="scm"
                     /locus_tag="ACGE4H_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8364928.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFL"
BASE COUNT         3844 a         2961 c         2709 g         4174 t
ORIGIN      
        1 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
       61 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
      121 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
      181 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
      241 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
      301 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
      361 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
      421 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
      481 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
      541 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
      601 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
      661 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
      721 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
      781 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
      841 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
      901 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
      961 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
     1021 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
     1081 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
     1141 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
     1201 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
     1261 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
     1321 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
     1381 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
     1441 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
     1501 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
     1561 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
     1621 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
     1681 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
     1741 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
     1801 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
     1861 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
     1921 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
     1981 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
     2041 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
     2101 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
     2161 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta
     2221 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
     2281 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
     2341 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
     2401 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
     2461 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
     2521 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
     2581 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
     2641 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
     2701 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
     2761 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
     2821 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
     2881 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
     2941 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
     3001 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
     3061 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
     3121 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
     3181 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
     3241 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
     3301 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
     3361 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
     3421 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
     3481 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
     3541 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
     3601 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
     3661 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
     3721 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
     3781 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
     3841 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
     3901 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
     3961 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
     4021 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
     4081 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
     4141 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
     4201 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
     4261 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
     4321 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
     4381 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
     4441 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
     4501 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
     4561 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
     4621 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
     4681 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
     4741 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
     4801 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
     4861 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
     4921 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
     4981 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
     5041 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
     5101 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
     5161 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
     5221 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
     5281 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
     5341 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
     5401 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
     5461 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
     5521 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
     5581 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
     5641 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
     5701 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
     5761 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
     5821 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
     5881 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
     5941 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
     6001 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
     6061 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
     6121 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
     6181 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
     6241 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
     6301 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
     6361 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
     6421 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
     6481 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
     6541 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
     6601 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
     6661 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
     6721 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
     6781 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
     6841 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
     6901 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
     6961 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
     7021 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
     7081 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
     7141 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
     7201 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
     7261 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
     7321 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
     7381 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
     7441 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
     7501 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
     7561 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
     7621 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
     7681 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
     7741 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
     7801 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
     7861 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
     7921 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
     7981 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
     8041 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
     8101 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
     8161 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
     8221 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
     8281 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
     8341 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
     8401 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
     8461 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
     8521 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
     8581 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
     8641 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
     8701 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
     8761 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
     8821 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
     8881 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc
     8941 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
     9001 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
     9061 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
     9121 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
     9181 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
     9241 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
     9301 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
     9361 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
     9421 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
     9481 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
     9541 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
     9601 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
     9661 tgatttctaa tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg
     9721 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
     9781 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
     9841 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
     9901 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
     9961 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    10021 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    10081 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    10141 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    10201 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    10261 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    10321 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    10381 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    10441 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    10501 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    10561 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    10621 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    10681 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    10741 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    10801 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    10861 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    10921 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    10981 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    11041 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    11101 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    11161 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    11221 tgaatctacg atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt
    11281 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    11341 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    11401 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    11461 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    11521 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    11581 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    11641 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    11701 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    11761 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    11821 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    11881 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    11941 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    12001 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    12061 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    12121 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    12181 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    12241 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    12301 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    12361 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    12421 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    12481 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    12541 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    12601 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    12661 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    12721 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    12781 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    12841 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    12901 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    12961 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    13021 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    13081 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    13141 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    13201 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    13261 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    13321 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    13381 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    13441 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    13501 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    13561 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    13621 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    13681 tttttatt
//
LOCUS       DAXWLB010000066        13130 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXWLB010000066 DAXWLB010000000
VERSION     DAXWLB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.066"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(82..777)
                     /locus_tag="ACGE4H_001511"
     CDS             complement(82..777)
                     /locus_tag="ACGE4H_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFF8364929.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4H_001512"
     CDS             complement(867..1967)
                     /gene="ychF"
                     /locus_tag="ACGE4H_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFF8364930.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1976..2167)
                     /locus_tag="ACGE4H_001513"
     CDS             complement(1976..2167)
                     /locus_tag="ACGE4H_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFF8364931.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2188..3078)
                     /locus_tag="ACGE4H_001514"
     CDS             complement(2188..3078)
                     /locus_tag="ACGE4H_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFF8364932.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3065..3832)
                     /locus_tag="ACGE4H_001515"
     CDS             complement(3065..3832)
                     /locus_tag="ACGE4H_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8364933.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4H_001516"
     CDS             complement(3844..4557)
                     /gene="rsmG"
                     /locus_tag="ACGE4H_001516"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFF8364934.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4H_001517"
     CDS             complement(4789..6690)
                     /gene="mnmG"
                     /locus_tag="ACGE4H_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFF8364935.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4H_001518"
     CDS             complement(6707..8104)
                     /gene="mnmE"
                     /locus_tag="ACGE4H_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFF8364936.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLNTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8403..8786
                     /locus_tag="ACGE4H_001519"
     CDS             8403..8786
                     /locus_tag="ACGE4H_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364937.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4H_001520"
     CDS             complement(8954..9712)
                     /gene="jag"
                     /locus_tag="ACGE4H_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFF8364938.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9730..10548)
                     /locus_tag="ACGE4H_001521"
     CDS             complement(9730..10548)
                     /locus_tag="ACGE4H_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFF8364939.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4H_001522"
     CDS             complement(10545..10901)
                     /gene="rnpA"
                     /locus_tag="ACGE4H_001522"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8364940.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4H_001523"
     CDS             complement(11019..11153)
                     /gene="rpmH"
                     /locus_tag="ACGE4H_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFF8364941.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4H_001524"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="ACGE4H_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFF8364942.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         3575 a         2808 c         2354 g         4393 t
ORIGIN      
        1 tattatttaa ctatggataa acaaaatgag gttctttact acccctagat atctagcaaa
       61 atagctgatc tttttgctag tttatcttct tgggacggat aaacttccaa taatcccgta
      121 ttttttcttc aagtagtaac ggattgctaa agcagttgcc ccgataatga taactactac
      181 tggatctaat actggattga taactggtgg caatagtgtt gtcgcagaaa acagtacgac
      241 ccaaagaagt gtcgttccac ctaggataag caatgtctta cccattcctg gtttcttgct
      301 tcgatctgct ccgggatatt catattgata gatgtatttg tacatcatat aaaagaccca
      361 gccaccgatt gcagaaccta gaacgagtgt agaaaagcca taagctggcg ttgttccacg
      421 tgaaaacaat ccggtaatcg caatcatgat acttaataga ccaagcaaca gcaacgtatt
      481 atctaaccac atcaataaag gggtggatga attttttact tctgctggct tattgatgat
      541 tgcttccgtt ctttcagaga ctgtcccaaa caattgacgg gctgttcttc cgcctttttg
      601 ttctgcaact aaagtaggca gaatgtcatg aagggctttt actttttctt cttcagacaa
      661 attcgctgct tctaaagatt ttttcaaatc aaaaatgtac tgttgatttc ttttggttaa
      721 ttttgtttct aaactattgt tttcttttac gattgctcgt aattgttctg gttccattat
      781 ctaatagtcc tccttagccg ttccttttct caacggtcgt cacaaaagac attctaccat
      841 attctacagc tagtttgata gcccttttac acattaaagc ggaataacat tacgtcgccg
      901 tcttgtacga cataatcttt tccttctaat cgaacacgtc cagcttcttt tgctgcatgc
      961 atgtttccgt aatgatccaa gtcttcaaaa gagacagttt ctgcacggat aaatccgcgt
     1021 tcgaaatctg aatggatgat tcctgctgct tgtggtgctt tgattccttt tttgaatgtc
     1081 caagcacgta cttcttgttc cccagcagta aagtaagtag ctaagcctaa caaatcataa
     1141 gcagcacgga tcaactgatc caaacctgat tcttcgattc ctaaagcttc taaaaattcc
     1201 gctttgtctt cttcatctaa ttctgcgatt tcttcttctg cacgagcact gaccacgatc
     1261 acttctgcat tttcttctgc agcaaagttg cgcacttctt gtacataatg attcgcgtct
     1321 gcatctgcta cgtcatcttc tgagacattg gctacataaa gaattggttt tgtcgtcaat
     1381 aaaaatagtg atttaacgat tttttgttct tcttcagtaa attcgatcgt tcgggcagat
     1441 tttccttctt ccagtacagg tttgatttta tccagtacag ctagttctgc cactgcatct
     1501 ttatctttgg ttttagcaac ttttgctaca cgagtatagc gtttgttgat agattctaag
     1561 tctgctaaca ctagttccaa gttgatcgta tcaatatctt ctagtggatc aacacgtcct
     1621 tctacgtgag taatattatc atcatcaaaa cagcgaacaa cgtgacaaat agcgtctact
     1681 tggcggatat ggcttaagaa ttgatttccc aaaccttctc ctttacttgc gccttttacg
     1741 attccagcaa tatctgtaaa ttcaaaagtc gtcggaacag tttttttcgg atgtacaagt
     1801 tctgttaagc gttgcaaacg ccagtcaggt acttctacca tccctacgtt ggggtcgatc
     1861 gtcgcaaagg gatagtttgc tgcttctgct cctgcttttg tgatcgcatt aaacaaagtt
     1921 gattttccta cgttgggcaa cccaacaatt ccagcagtca atgccataaa tttattcact
     1981 ctttctctgc atttttttca attacttttt ttaatttctt ttcaaaatct cgacgggtca
     2041 tcatgacgat atgtccgcaa ttcatacatc tgattttgat atctgcgccc attcgaatga
     2101 cttcccagcg attcgtctgg caagcgtgag gttttttcat ttcaacgata tcgtgcaagt
     2161 cgtacataaa aatagcctcc tttttgttta ttcttcatca aattgaatat tcaagatatc
     2221 aagaattcga gtcaaatctt tttgagaaag atattcaatc tcgatcttac ctttcccatt
     2281 tttttcttgg atagctacac ttgtaccaaa tttatccatc aaacgatctt cgctttctcg
     2341 gatataatat ggtttttcct ttgctagcct tgggattttt ttcttttctt ttccatcatt
     2401 ttgatttaac tcagaaacaa gctgttccag ttggcggaca gttaaatttt cagttactgc
     2461 acgattggct aatttcaaga tcaatgtttt attttttaaa cctaacagcg tacgggcttg
     2521 tcccatagat aaacgctgat cttgtaccat ttccttgaca agatccggta aagaaagcaa
     2581 gcgcaaataa ttggcgatat aaggtctgct ctttcctaaa cgactagcta cttctgcttg
     2641 tgttagtttc aggtttttca tcagcatctc atatgcttct gcttcttcta acggattcag
     2701 atcttctcgc tgcaagtttt ccagcactgc tacttgcatc atcgcttctt cgtcaaagtc
     2761 tctaacgata gcagggatcg tttctttttc agctagtttg gacgctcgaa aacgtcgctc
     2821 ccctgcaata atctcatatc cttttactgc ggaacggcgt acgatgatgg gttgaaagac
     2881 acctgattgt tcaatagaat tagctagttc ctgcaaagat acttcttcaa atgttttacg
     2941 gggctgatac ggatttggtc gcagctcatt caatggtagc tggacaacgg tatcatcttt
     3001 tacatccacc gtctctaagt tttctaaatc ttgaaacagt gcatcgattc ctcggcctaa
     3061 tcctttactt ttcttcacga cttaccactt cctttgccaa tgcttggtag acttcagctc
     3121 ctcgagaacg tggatcgtag tcgatgatcg gttttccatg actaggtgct tctgataaac
     3181 gaatatttct tggaatgatc gtttcgtaaa ctttttcgcg gaagtattta cgtacttctt
     3241 ctacgacttc atttcccaga tttgttcgtg catcatacat tgtcagtaag actccctcga
     3301 tctctaattc tggattaaag tgtttttgga ctaaacgaac cgtattcagt aattggctta
     3361 atccttccaa tgcgtaatac tcacactgaa caggtatcaa aatcgaatca cttgctgtaa
     3421 atgaattgat tgtcagatgg ccaagagaag gcgggcaatc gatcaaaata taatcatact
     3481 gactgcttac ttctgctaaa gatcctttta atcttgattc tcttgccatc attgacgtca
     3541 attcgatctc tgctcctgct aattggagag tcgcaggcac aatactcaaa ttttcgtgtt
     3601 cagtgatcaa agttgcctca tcaattggca attcattgac tagaacatca tagatgtctc
     3661 ttgtcacatc aggttttctg atcccgacac cactggtagc atttccttgt gcatccatat
     3721 ccactagcaa tacttttttg ccaaggctag caagacaagc acctagattc accgtcgtcg
     3781 ttgttttgcc gacgccacct ttttggttcg caacagatat tatttgtgcc atctcttctc
     3841 ctcctatttg attggctgct tattaggcag tccgggcttt cttgggtatt tctttggggt
     3901 tggtttcttt ttttgaataa cgatgatatg ccgttgatca gagacattcg gcaaggtaac
     3961 cgcctcttct ttgaggaatt taccacctaa taaagcaatc gcttgttttg cttctgtcaa
     4021 ttcctcttca ctttttgctg ctttcaatgc ataaaaatag ccttcttgtt tagccaaggg
     4081 caaacacaat tccgctaaca cattcaaacg agcaaccgca cgagcagtga caaggtcaaa
     4141 tgatgcacga aagtgaggat tttgcccaaa tgtttctgcc cggtcatgat ataaggaagc
     4201 atcgattcct aataatcggg ttaactcaga taagaaggtt attcgtttat tcagagaatc
     4261 aacgatcgtt atctgcagtt ctggaaaggc aatttttaaa ggtatgctag gaaaccctgc
     4321 ccccgaacca acatcgcata aagataaagg ttgcgtaaag tctacttcaa atgctaatgt
     4381 aagtgaatcg taaaaatgct tcagatatac gtctttttca tccgtaatag ctgtaaggtt
     4441 catcttttcg ttccattcga ctaatgtccg ataataaata gcgaattgat tcatttgatg
     4501 atcggtaagc tctatccctt tttctgccaa ggattggcga aattcttctg gtatcataaa
     4561 tattcctttc attgtgaaac agcaattgtt tcacagattc tctccctact ttaacgcaaa
     4621 atctctagac atgcaatcct tccatatgga ttcttaaaaa tttgaaaagt aagaaaagcc
     4681 gatagacttt ttctttctag tatgactcat aaaatataca cagaagagag aactagcttt
     4741 gagcaatttc tgcaaaaact agttctctct tctatttatt attacgtatt acgctggttc
     4801 actgactttc gcaatcttcc cttgttcgat atagaccatt aggatactta ggtctgcagg
     4861 gtttacgcca ctgattcggc tagcttgggc aattgtttct ggttggattt tctgcaattt
     4921 ttgttttgct tctgttgcta atccgttgat tgctgcgtag tcgatgtttt ctggaatctt
     4981 tttcgcttcc atgcgtttta atttttctac tttttccatg gcttttttga tgtagccttc
     5041 atatttgatt tggatttcta cttgttcgat ttcttttcgt gtcaattctt cattttctgg
     5101 aataaaccgt agaagttctg cataagtaat ctcaggacgt cttaaaaagt cgcttgctag
     5161 tacaccatct tttaatggtg ccgcattaat cgttgctaaa aagtcttgga cttcttctgt
     5221 tggtttgatt cgtacattca tcaaacgttt gatttcttgt tcaactgctt cttttttctt
     5281 caaataattc ttgtattgat cttcttttac taagcctaca cggtgaccca tttctgttaa
     5341 tcgcaaatct gcattatcat gacgtaaaag cagacggtat tcagcacgtg aagtcagcaa
     5401 acgataaggt tcgttcgttc cttttgttac caaatcatcg atcatgacac cgatgtaacc
     5461 gtcacttcgt ttgatcacta aaggttcttt tccctgaact ttcaaagctg cattgatacc
     5521 ggcaatcagc ccttgtccag ccgcttcttc ataaccgctt gtaccatttg tttgtccagc
     5581 tgtataaaga ttttcaacga ctttagtttc taatgtcgga cggagctggt gtggctctac
     5641 tacgtcatat tcgatagcat atcctgtacg catcatttct actttttcta atccagcaat
     5701 agaatgaagc atgttgactt gaacatcctc tggtaaagaa gtagataaac cttgaacata
     5761 gacttcttcc gtatttaagc cttctggttc caaaaataat tgatgacgtg gtttgtctgc
     5821 aaaacgcacg atcttatctt cgatggatgg acaatagcga gcgcctaccc cttcaacgat
     5881 ccctgtaaac attggggcac gatgtaagtt gtctcgaata atttggtgag tcgtctcacc
     5941 tgtataggtc agccagcaag attcctgatc tagtttatag gcactatctg gtgtgctgaa
     6001 gctaaaatga ttcggctctt tgtcaccagg ctgctcttct gtaacagaat agtcgatcgt
     6061 acttgatttt actcgaggcg gtgttcctgt tttgaaccgt tcgatagcaa agccaagttc
     6121 ttttagatga ttcgctagtc caatagaagg ttgagagtta ttcggacctg atgaatattt
     6181 caattcaccg ataatgattt ctccgcgaag tgctgttccg gctgtaataa caactgcttt
     6241 ggcccggtag ctcgctcctg tggacgtcac tactccatga caaacaccat cttcgacgat
     6301 cagttcttcg acgattcctt gtcttagcgt caaatttggt tctttttcga tcgtatgttt
     6361 catttctgtt gaataagcat gtttgtctgc ttgtgctcgc aatgctcgga cagctgggcc
     6421 ttttcctgtg ttcaacatac gcatttggat ataggtttta tcgatattct tacccatttc
     6481 tccgcctaac gcatcgattt ctcgaacgac aacgccttta gctggtccgc ctacagaagg
     6541 attacatggc atgaatgcta ccatgtccaa atttattgtc agtaataatg tttttgatcc
     6601 catacgtgca gcagcaagtg cagcttctga accggcgtgt cctgcgccaa caacgatcac
     6661 gtcatacgca tctgcttcat aatgttccaa tcgtttgttc ctccttttat tttcctaaac
     6721 aaaattgact gaataattga gtgattagct catcttgtac actgtcgccg acaacttctc
     6781 ctagatagtc ccagcaacgc gtcatatcta tttgaacgag atccacaggc attcctgaat
     6841 caatacctgc aatgacttct tcaagtgaga gggaagcttt ttctaacaac gcgatatggc
     6901 gggtattaga aacataagta gcgtctcttt ctcctgtttc tccgctgaag aataaatcag
     6961 aaatagctga ttccaaacga tctagtccgt cgtttttagc tacggaaatt gaaaaaactg
     7021 gttcattttc gatcaatttc ttcaattttt cttgttccag ctgtgctggc aaatctgttt
     7081 tatttagaag aatgatccgt ttcaatccac ttgtcgcttc caataattgt tcatcttctg
     7141 ctgtcagtgg ttcgctttgg tttagaacaa gtaagatcaa gtctgcttct gctaatgctt
     7201 ttcggcttct ttcgaccccg atccgttcca ccacatcttc tgtttcccga attccagctg
     7261 tatcgattag cttgagcggt accccccgaa cattgacata ctcttcgatt acatctcgag
     7321 tcgttcctgc aatatccgta acgattgctt tttcttctct taacaaatgg tttaacaaac
     7381 ttgatttgcc gacatttggc cggccgatga ttgccgtatt taacccttcc cgcagaacct
     7441 tcccttgctt ggatgtagca agtagcgctt ggattctttg ttggatcatc tgcgcttttt
     7501 caagcaataa cttcgtagtc aattcttcga catcatcata ttctggataa tcaatattta
     7561 cttctacttg tgctaatgtc tctaaaatct cttgtctcaa tgagcggatc aaagcagaca
     7621 gattgccgtc taactgatta agagctagtc ccattgcttt gtctgttttt gcccgaatca
     7681 aatccatcac agcttctgcc tgtgaaaggt caacacgacc attcaagaat gctcgttttg
     7741 tgaattcgcc tggctcagct aatcttgcac cttctcttaa caatagctgc aaaatttgat
     7801 tgactacaac gatcccaccg tgacaattga tttctactac atcttcccgc gtaaatgtct
     7861 ttggtgctct catcacggag accatgactt catcgacaag ctgattgctc ttgggatcaa
     7921 cgatatgtcc ataatggatc gtatgacttg gtacttctga taatcgtttg tttccagatt
     7981 gataaacttt atctgctagt tgcacagctt gatcgccact taaacgaacg atactgattg
     8041 caccttctcc aggtggggta gaaattgccg caatcgtatc aaattctaaa gtaatttcag
     8101 ccattttttc acttcctttt tcattttgcg cacaaaaaaa gtgcccactc caccctttat
     8161 gcacaataaa cggtgaactt cgcactttga ctgcttgttc ttgattaaat tctggtcata
     8221 gattatcaca cttgctataa ggaatcaagt gtttaaaaca attagatgaa gttgagcctg
     8281 ttctgcttcc attaccttat atttaattgt ccttggatgt ctctttgcgt tctttggttt
     8341 attcgaagag aagatactgt ataaacaagg ctttatcttt cttaaaaaaa acggtacact
     8401 aaatggtagc gctatctaaa ctatacactt ttgaaaggag aaaatatgtg agcccgttca
     8461 gatctgctgc attggaagaa gaaagtgacc ctcggatcaa cgatgtcgtt ttatctacag
     8521 ggaatatcaa ccagaaatat atcgtccgcg atatcgtttt tgttgtggaa tgcttagaag
     8581 ctgatttgtt tgacccatct tttcccaaag atgacctttt cttaacaacg aagaggaaat
     8641 taaagacgaa ggtacttgaa tatggtgcga atgcagtaat caactgtcat tttgagtatc
     8701 agcaaaaaaa acgcgacgga aagtattttc tggaactttt tgcttatgga acagtgatcc
     8761 aatttatcca aacaacgatt ggttaaatga aacagacaaa aaaagcggat caaaactgaa
     8821 gacagtttcg atccgctttt ttctatcaaa gttagtgact acttcatcgt atactctgat
     8881 aagaatgatt tctagtaaaa tatacttggt ttgaaaaact ttgtcaacgt gatttattca
     8941 aaaagtaatg gaatcaataa tattttttag caggttctac tactaaataa cgataaggtt
     9001 cctcaccttc cgaatgtgtt tttacatgtt catctttgct taatgcagca tggatttgtt
     9061 tacgttcaaa tgcaggcatt ggttctaaga aaacaggctg ccctgtccgt tttacttttt
     9121 cagccgtgcg atcagctaaa cgctgaatga tctcttggcg cttttcacgg taattgccta
     9181 cattgactac tacagacagc ttatttcttg ctaaacgatg gatgaagact tgtgccagat
     9241 attgcaatgc attcagtgtt tttccatgtt tcccaatcag tatgccttgc ttttgtgttt
     9301 ctaaatggaa aacaaccata ttatttgatt ctctttccat tttgacaaga gctggagcat
     9361 caagctgttt tgaaatttca gtaagataaa ctgccaattc cgttaaagct gcttcatcac
     9421 ctagctcact tacttcttct gttttttctt caagaatcgt ttcgttgact acctcgatct
     9481 cagctgttgg ttcttcttcc aaaatttctt ctacagtctg ttctactgtt tcagaaactt
     9541 tttcgctgat acttggctgg atcgaaagcc gtgcattctt tcgacccatt cctaggaaac
     9601 ctttttttcc ttcttccagt atttcgattt cgacttggtc tttggccaaa cccaaatcag
     9661 ccagacctag ttggacagct tcgtctattg ttgcagcttc ataaatcggc attagcatta
     9721 cctcccctat tatttccgtt ttttcttagg attcattgct ttcttaagag cacgttctct
     9781 ttcgcggatt ctccgtgctt cttcttcacg ctcttttcga attttgaaag ggttgttgat
     9841 cagcagcgtt tgtacaactt ggaaggcatt ggaaactacc cagtataatg aaagaccgct
     9901 ggctaaattg acacccatca ctaaaatcat tacaggcatc acgaaattca tgatcttcat
     9961 tgatgcattc gattcaattt gactcatgct agataaatac gtacttgcaa aagtaaagat
    10021 tgctgctaaa atcggcaaaa taaatgttgg atccgcgttc cctaagttca accataagaa
    10081 atgcccttgt ttcaactctg gtacacgtga aatagattgc cataacgcca tcaagattgg
    10141 catttgtact aataaaggaa gacaaccagc atatggattg acgccatttt ctgcatataa
    10201 acgctgctgc tcttctctta acttactttg tgtttccgga tctcttgaac tatattgttg
    10261 ctgcagtgct tttaatttcg gttgtaattc ttgcgtttta cgcatgctct ttgtttggaa
    10321 atgcatcaac ggcaatagga tcacgcggat gatcagtgta aataaaatga tccctatccc
    10381 tgcattacca aatgacaacg ccttgatcgc ctcagcaaag taataaacaa tataacgatc
    10441 ccaaattcct gtactctgtg cgctgacttc cccagttccg caaccggata agacgatgac
    10501 caaactaacg ataccggcca tcaaaagtaa acgtttgtat ttcttcactt actcttttcc
    10561 tctctgtcga ttatttttgc aagttttaag acgtggacta aattggagcg aacctcatca
    10621 taagtcaacc ccttcacact tggtctagca atcacaataa aatccaactc cggatcaatt
    10681 tcttctttta tagaataaac tgctgctcgg atttttcgtt tgacttcatt gcggtttacc
    10741 gcgttgccga ccttttttcc tacagataaa ccaactcgaa aatgaggctg tcccggtttt
    10801 tccaaacggt aaacgacgaa tttgcggtta gcaaaagaat ctcccgtatg gaacacggct
    10861 tgaaactctc gttctttttt tactcgatac gcttttctca ttagttttct ttccttttct
    10921 atctctcact tacgctcata atcataccat aattacgatt gtcctttcat catttcccta
    10981 aaaaagagta taaaaaaaac cactgaaacc ccagtggcct aagctgaaag aacttttctt
    11041 cctttgcgac gacggctagc taacacacga cgtccatttt tagtactcat acgtttacgg
    11101 aatccgtgaa ctttttgacg tttacgttta tttggttggt atgttctttt catatattcc
    11161 acctccaaaa tgttcttatc tattttataa acacagacat actttgatag tataacgaca
    11221 aagtagtgat tttgtcaata taaaatagaa gatcgattgt taaaacttat ccacaagatt
    11281 atttttttct atgggaatcc tatgtggaaa aagttatgca cagggattat ccacagataa
    11341 taaaaggctg gttttacaca gaaaacacac gtgtaaaaaa agtaatccac aatcaggcga
    11401 tttcctgtgt attccgtctc ctaactattg gtatatctgt attttttatc cacagacgca
    11461 agaggaaatc tattggtttt tacgagtttt ccacctgtgc attagtggtg tggaaaactt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgccttg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatctcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atacacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac
//
LOCUS       DAXWLB010000067        13084 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXWLB010000067 DAXWLB010000000
VERSION     DAXWLB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.067"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(78..150)
                     /locus_tag="ACGE4H_001525"
     tRNA            complement(78..150)
                     /locus_tag="ACGE4H_001525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACGE4H_001526"
     CDS             531..1100
                     /locus_tag="ACGE4H_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFF8364943.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACGE4H_001527"
     CDS             1087..1947
                     /locus_tag="ACGE4H_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8364944.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4H_001528"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACGE4H_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFF8364945.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4H_001529"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACGE4H_001529"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFF8364946.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4H_001530"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACGE4H_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFF8364947.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACGE4H_001531"
     CDS             5995..6864
                     /locus_tag="ACGE4H_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFF8364948.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACGE4H_001532"
     CDS             6919..7728
                     /locus_tag="ACGE4H_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8364949.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="ACGE4H_001533"
     CDS             complement(7831..8460)
                     /locus_tag="ACGE4H_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8364950.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="ACGE4H_001534"
     CDS             8630..9922
                     /locus_tag="ACGE4H_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8364951.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="ACGE4H_001535"
     CDS             complement(9998..10660)
                     /locus_tag="ACGE4H_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8364952.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /gene="groES"
                     /locus_tag="ACGE4H_001536"
     CDS             10885..11169
                     /gene="groES"
                     /locus_tag="ACGE4H_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFF8364953.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="ACGE4H_001537"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="ACGE4H_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFF8364954.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4343 a         2261 c         2829 g         3651 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aatt
//
LOCUS       DAXWLB010000068        13054 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXWLB010000068 DAXWLB010000000
VERSION     DAXWLB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.068"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(3..1304)
                     /locus_tag="ACGE4H_001538"
     CDS             complement(3..1304)
                     /locus_tag="ACGE4H_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFF8364955.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1534..3525)
                     /locus_tag="ACGE4H_001539"
     CDS             complement(1534..3525)
                     /locus_tag="ACGE4H_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFF8364956.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3669..3935)
                     /locus_tag="ACGE4H_001540"
     CDS             complement(3669..3935)
                     /locus_tag="ACGE4H_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8364957.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4H_001541"
     CDS             complement(4445..6571)
                     /gene="pnp"
                     /locus_tag="ACGE4H_001541"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFF8364958.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4H_001542"
     CDS             complement(6759..7028)
                     /gene="rpsO"
                     /locus_tag="ACGE4H_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFF8364959.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7279..7842
                     /gene="def"
                     /locus_tag="ACGE4H_001543"
     CDS             7279..7842
                     /gene="def"
                     /locus_tag="ACGE4H_001543"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFF8364960.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8095..8616)
                     /locus_tag="ACGE4H_001544"
     CDS             complement(8095..8616)
                     /locus_tag="ACGE4H_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFF8364961.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8619..9461)
                     /locus_tag="ACGE4H_001545"
     CDS             complement(8619..9461)
                     /locus_tag="ACGE4H_001545"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFF8364962.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9454..10179)
                     /locus_tag="ACGE4H_001546"
     CDS             complement(9454..10179)
                     /locus_tag="ACGE4H_001546"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFF8364963.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10355..10651
                     /locus_tag="ACGE4H_001547"
     CDS             10355..10651
                     /locus_tag="ACGE4H_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364964.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      10907..11081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            11219..12811
                     /locus_tag="ACGE4H_001548"
     CDS             11219..12811
                     /locus_tag="ACGE4H_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8364965.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12824..>13054
                     /locus_tag="ACGE4H_001549"
     CDS             12824..>13054
                     /locus_tag="ACGE4H_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8364966.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLP"
BASE COUNT         3721 a         2740 c         2476 g         4117 t
ORIGIN      
        1 ttttattcta tttttctttc cgtggactct actttgaaat aatcgatatg tgcatgctgg
       61 tattctaata ccgtgaaacg gaatgatgca atctcaatga ttgttcctgc ttttatttct
      121 ttatatcggc tagaagcaaa gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga
      181 atctctacat gaaaataacg ctggaaatca tctaatgtta gtttgccgga gatgatataa
      241 gagccatctg actgttccgc tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc
      301 ccgacgattt cttctaaaat atcttcaatc gtcactaagc cgcttgtccc tccgtattca
      361 tcttttaaga tagcaatatg ctttcccttt tttcgcatga tcgtcaataa tttttttata
      421 gggatcgttt cagttgttac gatcggctct tcaatcaact gttctactgt cagctggtca
      481 tctttataag attgcttgat caaatctggc aaggtgacat aacctaaaac attgtctttt
      541 gattctaaga taaccggata gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct
      601 ttgacggttt ctgaagcttc gaatacttgc atggacgtac ggtctacttg tatatctttt
      661 gccaaggtat catcaaattc aaacacgttt tccaaataat gatattcttc tttggtcaac
      721 tctccttttt cataagaatt caatgcgact aatgtcagct cttcttgaga caatgtctct
      781 tctccttcag atacgagacg cacacctaat agcttcccta gccctgcagc tgaatgattc
      841 aatacccaaa taaaaggaaa cattacttta taaaaataat gaagtggttt aactacgaac
      901 aagacgacct tctccgtatt gacgatacta tagctcttag gaagcagttc gccaataacc
      961 acatcgatat acgtaactaa aataaatccc agcgccacag agattgtact gctaatagcc
     1021 gaactaaaag gtagtttacc gatcactggt tctagtaaag ccgcgacaga tccttcaccg
     1081 acccacccga tgatcaaacc ggttagcgta attcctaatt gactagctga taaatagtta
     1141 tctaaatggt gtgtaacatg aagagctagc ttggcgttct ttacattttc cttttctagt
     1201 tgttccaatc ttgatcgtct gattttcact aaagcaaatt cacttgctac aaaaaatgaa
     1261 gtaattgcaa tcatcaaaaa taaaataatg aaattgataa ccatattctc ccgcctttct
     1321 ctttttctat tgtacccttt ttctacaaaa agattgaaaa taatgcgtat tcatatttag
     1381 gaataaaaaa ggagatgcgg catttttcag ccacatctcc ttatcacttc aaaatcattg
     1441 cgttacttgt tgctaaacag ctttttcatg acctctgata aacggtgttc aagtagctga
     1501 cattcgacat tagttcaggt caaagcgcta ctttcacaac cgctctttca accatgtttt
     1561 ccgagtattt ccaatgataa gcggaacagt ttcatgtttt acttcgctgt actctagccc
     1621 aaaccatgtt tctggcgagg tagcgatttt tttgattgcc agttttgcct gcatgatttg
     1681 acgttgccat tttcctagag ttgtcacatt ggaaactact tcatcaatca ggacaaattg
     1741 gaaatcgcca acttctcgcc ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg
     1801 gccttgtttc atcaaatcat ggatgacttg gcgaacatat acgttgattt cctgttggac
     1861 cctgaaacct aagaacagat tcacttggac aataaaatca gtgtccatca tatctacttg
     1921 atattctttc gtatatggct catccgtcac ttccacatga acaaaccaat agactcgtgc
     1981 tcttttaggc cgtttatcta aaatcgaata gatgaactgt cggccaactt tatcttcagc
     2041 catatcagga accaagaaga ccacatttgt ctgatagatt ggtaaagaag tatcatcacg
     2101 taattctcta agctgcggga tataatctct caaagacaca tcctcggttg acgattcgcg
     2161 aataatgttc ccttgttccc aaataaacat cacgactaag atgatcaaac caatcagaac
     2221 ggctacaaaa ccgcctcgga agaatttgac aacacttgag ataaagaaaa cgacttctat
     2281 cgcaccgaag aaaaaggtaa ctaaatatgc aagccaacga gaatgcccat tttggataag
     2341 ataaaagtaa agcagtgctg ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc
     2401 agcttccata tgatgagacg ttcggaatgt gacaaccact agagaacagg cgatccataa
     2461 aatcagattg actgctggga tatacatttg cccgatactg ttcccaggat acatgatctt
     2521 taaccgaggc agcagtttca acttgattgc ttctgaaact agcgtaaacg aacctgtaat
     2581 caatgcttgc gaagcaatga ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca
     2641 actttgaggc atcatttgga agaatggatt caggttcttg atttgctgtg cttcagggtt
     2701 ttgataaaca ttcaacaacc acgcagcctg cccaaaataa ttgataacca aacaaatttt
     2761 gacgtaaggc cagctcagac gaatatttcg tttgccgaca tgtccaagat cggaataaag
     2821 cgcctctgca cctgtcgtag ccaggaaaat atttccaaga atgaacaagc ctaatttgtt
     2881 atctgcacta aaaagcagct ggatagcata ataaggattc agtgcacgga taactgtcca
     2941 gtcttgactg aagttcatta atcccattac gcctaaaaaa gtaaaccaag cgaacatgat
     3001 tggtccgaat gcttttccga caatatctgt accaaagcgt tggaccatga ataaaacaaa
     3061 aataataact aaagtgataa tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa
     3121 ctgcggaatt cctcgcaagc cttcaatcgc agtcgttacc gtcacagcag gggtcaatac
     3181 cccatctgct agtaaggcag ctcctccgat cattgcagga atgatcaaat atttcccacc
     3241 ttttcggaca agtgtgtaga gtgagaaaat tccaccttca ccatggttat ctgcattcaa
     3301 tgcaatcacg acatatttga ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac
     3361 tgatccgata acaaaatcag gattgattcc tgacaatccg ccattatctt caacaatcga
     3421 tttcataacg taaagcggac ttgtcccgat gtctccgtag acgactccca tcgctacaag
     3481 caggccagcc attgatatct ttttggtatt gtgcgttgtt gccacgtttt tccctcattt
     3541 cttgttatat ggatatcctt taggtaagct tgaatgatag atgatattct tatccatgac
     3601 acccgctttc ctaaattgct tctaattatc caagtaacac actgaagtgt caacttattt
     3661 gaataaaatt attctttctt tggacgttct ctgatacgtc ttgccacaaa tgaataaaga
     3721 gcaaggcaaa aaattgcacc caataatgct ggtatcaaat aaaaaccgcc gagttccggt
     3781 cccagtggtc ctagtaaaaa ttcgccgagc caactaccta aaaaaccgat aatgatattt
     3841 ccagatacgc ctcccgggac ttcctttcct aaaattgcgc cagctatcaa acctaatata
     3901 ccgccgataa ataacgacca taaaaaattc agcatctcct ttccctcctt tatccatatt
     3961 tctcactttt attttacgct ttattcagaa ggactccaaa aatatgcgcc aagaaactcc
     4021 agaaaaaaag aagttgtaac aaaagtgttc agcttatttc cgtagaagct gcttctggaa
     4081 caccgtgaat cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg
     4141 aaaaatagtt cctcatattt tgccgttttt tgagctaata tcgagggaca gctgattgaa
     4201 catcgtttat tggagaggtt gtaaaaaagc tgttctgcat caagtaataa gaacagccaa
     4261 gtaaaaacag ctcctaatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
     4321 tgaacaccgt ttattcgaaa aaaaaggact gtgacacggc ttttgtcaca gtcccctttt
     4381 ttcgaataat tattgtcccc ctgtcacctt cacgatcaca agtacattat tcctatgaat
     4441 taaattattt ttcttcattt ttcgttgaac catcttcgtt caacattgct tttcttgaca
     4501 gatttacgcg tccttgttta tcgatttcag ttactttcac aaggatctca tcacctagtt
     4561 taacaacatc ttcgacttta ttgacacgat cgtttgctaa ttgggaaata tggacaagtc
     4621 catcttttcc tttgatcaag ttgacgaatg caccgaattt ttcgatacgg acaactttcc
     4681 ctagataaac ttcaccaact ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt
     4741 ttttgatcat ttctgcatca gctgaagcaa tacttacttt tccatcttga tcgatatcga
     4801 ttttcacacc agtttcatcg atgattccgt tgatcgtatc gccgccttta ccgataacat
     4861 ctttgatttt agcaggttcg atctggatca tttcgatttt tggtgcatat tggcttaatt
     4921 cttcacgtgg agcagcaatc gttgaagtca attcttctaa gatttccata cgtgcttgtt
     4981 ttgcttgagt taatgcttca gttaggattt gttctgtgat tccttgaatc ttgatatcca
     5041 tctgtaaagc agtgatacca tcttttgtac cggcaacttt aaagtccata tcgccaagat
     5101 gatcttccag cccttggata tcagttagga tcgtatagtt ttcaccatca gaaacaagtc
     5161 ccatcgcaat accggcgact ggcgctttga ttggtacacc tgcatccatc aatgctaatg
     5221 tgccagcaca gatacttgct tgggaagaag aaccatttga ttccaatact tctgctacta
     5281 aacggatcgt ataaggaaat tcttcttctg aagggattac ttgtgctaac gcacgttcac
     5341 ctaatgcgcc atgtccgatt tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa
     5401 cagaaaattg agggaaattg tagtggtgga tgaatcgttt gctgacttcc acccctaaac
     5461 catcaatgat ttggtgctct cctagaggtg cgagtgtaca agctgataaa gcttgtgttt
     5521 gtcctcgtgt gaacaaacct gatccatgta cacgtggcaa taagctgacc tctgatgaca
     5581 atggacggat ttcatccaat ttacgcccgt caggacggat cttatcgatg gtgatcaatt
     5641 cacgtacgac atctttttca agatcttctg tgatttgttt aacttctttc aacaagtgat
     5701 cttcatcttc atgacctgca aatttctcag cataagcttc gcggatatcg atcttcacct
     5761 gctcgatatt atcttcgcga gcaagttttt cttcagtcat tactgctgct ttcattgtag
     5821 cgtaagaagc atcaaagatt tcttttttca atacttcatc aacttgcagt aaagtcactt
     5881 ccattttttc tttccctact gcttgaacga tttcttcttg gaaagcaact aactctttga
     5941 tagcgtcaaa accaaacagc aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc
     6001 cgctttcaac catattgatg gcttgtttcg ttccggcaac agtcaattcg atatctgttt
     6061 gttctgcttg ttctactgtt ggattcaaga cgtactcgcc atttacacga ccaacttcta
     6121 cgcctgcgat tgggccgtca aatgggatat ctgagattga tagtgctaaa gaagaaccta
     6181 gcattgctgc catagctggc gagcagtctg tttctacact catcacgatg ttcgttactt
     6241 ggacttcatt acggaagcct tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg
     6301 cagtcaatgt tgcttccgta cttggacggc cttctcgttt gatgaaacct ccagggattt
     6361 tccctgctgc atacattttt tcttcataat tgatcgttaa tgggaaaaag tctgtatctt
     6421 ttgcttcttt agaggccaca gctgcactta agactactgt atctccataa cggactaaaa
     6481 cggctccgtt tgcttgtttt gctagctgac cgacttcgat ttgtaaggga cggccgcccc
     6541 atgtcgtttt gaatacttgt ttttctgaca taacttctcc ttttccttct cgtgaaagca
     6601 agacgctact aaacaaatga aaactgccga aagccgtctt ttgagcagct ttcatttggt
     6661 tagtaggagt ccatctagct tgatcacgga attttactta attgatatac gagtaaaaaa
     6721 agtgagattc atgtgaatcc cactcttttt gactaaaatt aacgacgcaa gcctaaacgt
     6781 ttgatcaatt cgcggtaacg ttgaacgtca gttttacgta agtaagctaa caagttacgg
     6841 cggtgaccaa cttttttcat taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt
     6901 gcatgttcat tcaagtggtt gatttcttca gttaatacag cgatttgtac ttctggagaa
     6961 ccagtatccc cttcatggcg agcgtattcg ttgatgattt cattcttgcg ttcttttgaa
     7021 attgccattt ctttcacctc tttcgattat tcccctgcga ctgagtaaaa cgttggtgat
     7081 tcgcttaacc aagtggcagg tcatgtgcct agagcacaca gctatacttt acctgaaacc
     7141 gggttgaaaa tcaagtagtt aatcattaag aaaaggtagt tttcaaacaa tcttactact
     7201 acttttccat ttttgtggta aactataatg cgaaaataga cttgaaacac agtcttttaa
     7261 gaaaggggta catattccat gattacaatg gatgatatca ttcgtgaagg aaatccgacc
     7321 ttacgcgaag ttgcaaaaga agtttctctt ccattaagcg aagaagatat ctctttagga
     7381 aaagagatgc ttgagttctt aaaaaacagc caagatccaa tcaaagctga agaactacac
     7441 ttacgaggcg gtgttggctt agctgctcct caattagata tttctaaaag aatcattgct
     7501 gttcacgtac caagtcctga cccagaggca gatggacctt ctctaagtac tgttatgtat
     7561 aatcctaaaa ttttgagcca ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt
     7621 ttatctgttg atcgagaagt accaggatat gttgttcgcc atgccaaaat cactgtgtct
     7681 tattatgata tgaatggtga aaaacacaag attcgtttga agaattatga atcgatcgtc
     7741 gttcaacatg aaatcgacca tatcaacggt gtgatgttct atgaccacat caacgatcaa
     7801 aatccatttg cactaaaaga aggcgtattg gtgatcgagt agagaattgg caggttcctt
     7861 cgatactatg ctacaagaaa gctttataaa aaaggggagt gctcaaaagc tgcacttccc
     7921 ttttttatgt ttctccacta atcgaaattt agatagacat ctagaaatcg caattccaaa
     7981 aaattctgtg tattggatac gcatcttttc ttattttttc ttatttcttc catatgggtg
     8041 tttatctcag agatgataga atagtttcta ggataaaaag tagcagctac gatgttatcc
     8101 ttcgtagagg attcctgctt cagctagttt ttgtttgatg tttttcagca tatgcttttc
     8161 tgggtagcgg atcgtatgca gatggatgcc gcctgttaat tcggataaca aagtagcatt
     8221 acttttttga taacgtctca taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt
     8281 tcctgctaac tcaccgtaaa ggggatgttc cacaattaca tcaatgattt caccaccatt
     8341 ttcaacgatt agtctcagtt cttcttctgt ttgatctttt ccatgctgga cagcgatttt
     8401 actgatgtgc ccagtattgg tttcttctgt ttcgaggata taacctcttg ctgttgccac
     8461 gatactttta cctgcagctc gtagtaaagc aatatcgcct acaatgatct gacggctgac
     8521 tccaaaagtt ttcgcaaact tgctagcact cactggtttt tctgtatttg ccaaggtttg
     8581 catgatttgt tttcgccgtg tctctccttc gatcacactt attcctcctt cattttgttt
     8641 tggtattcgc gcgagtgttt catcaaatca acatacgtat ctgttattgt ttccttatat
     8701 cccttatttt tcagcagact aacaacttta tccaatactt gctgctctct ttttgcatca
     8761 taaactgctt ggttttcttc ttttttcact tgtcctattt tagttaccgt atccattctt
     8821 ttttctaata aagcaaccaa ctctcgatcg atcatatcga tcttatgacg taatgtttca
     8881 agagactctt ctgttagttc ttcctctact gccttgatga tacgagcagg atttcctgca
     8941 atcactacat tgtcgggaaa agatttggtc acaaccgaac ctgcgccgac tactacgttg
     9001 tcccctagag tgacacccgg cgtgacgata actcctccgc ctaaccaaac attattaccg
     9061 attttaatgg gttttgcgta ctccagaccg ctgttacgtt tcactggatg tagtggatgc
     9121 gtcgccgtgt aaagctggac attcggagcg aacatgcagt tatcaccgat ttcaatcgtg
     9181 ctgacatcta aaaatgtaca attgaaattt gcataaaaat tctccccaac aaatatatta
     9241 tatccgtaat caaaattgat aactggctcc atatagattt ttttccctgt acggccaaaa
     9301 gtctctttca acaattggga tcgcagttcc gctgattctg cttggttgat gatttggctt
     9361 tgacttcttg caaacatacg gtcggcaacc agctcaggat cattggcaaa ataaagctcg
     9421 cctgcaatca ttttttcttt ttcggttttg aattcagtca taatctgctc ctaattcttg
     9481 tatttttttg aaaacaaccg ctccgtcttc ttgaataaac gtataaggct ccgctaaata
     9541 aacaggcgtt tgattgatta ctaagatttt ggctgacggc tggcgaaact gtaccagatc
     9601 acaaaacgga tgaacttgaa aactcgttcc aacaatgacg atcagctctg cttggctgac
     9661 ggcataagcc gcctgctcca tgactccatc ttgaaaccct tcaccatata acacgatgtc
     9721 tgggcgtatt tgtcctccgc atgcttgatg tttgtcgctg tgaagatatt ctttccagtc
     9781 taccgtttgt tgacattttc gacaatgaca ttgatacaaa cttccatgaa aattgacgag
     9841 ttgcgggctt ttagcctttg cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt
     9901 tttttctttc gctagatgtg ccatttccaa atgaatgata ttgggttttg cttcaggatg
     9961 ataaatcttt ttgacaaatt gatagaattt atccggttca ttcaccattg cttgatgact
    10021 cagcaagtat tccggctgct ccatcccgtg gtagattcct gacaaagatc gatagtctgg
    10081 cactccagaa ggtgtggata cccctgcacc ggtcaaaaat gtaatttgct gataagctaa
    10141 tatctctttt gctgcttcgt ctactgttaa ttgtttcatg ttttgtcacc tcactttcca
    10201 ttatagccca tactcgaaca aatttattgt tgaaaagtca gaaaaatggg aataaaaaag
    10261 tgttatgggc atttgaacaa tttatcacaa tgttcactga caaaagaatg aaaacgtgtt
    10321 acaatactgc tgtataaaag tgaggtgaat cgatatgtta ccattcttta tttattgggg
    10381 cgttattctg gcttgtatcg cctggttagc gttaagcgtt tatttctctg ttttttatct
    10441 tgtacgcaaa gaaaatggaa atctttgggc ttttgcttta ttcaacgtga ttgccgcaat
    10501 tgttttagcc attacattag ccgtatatcg aacatggggc tggggaatca cccaatactc
    10561 aagtttgatt tatctaatat tagccattta tggcgtcgtt gttcttttgc aagcgatcct
    10621 aggacgtgag ccaaagaaag cggcagctta aatgatcgag atggaactgg acacaggaaa
    10681 ttttctgttc tagttctttt tttattgtat tacatacatt tttgtgtata attatactat
    10741 cgtttgcaat gaatagatga ttcacatttt gattgttttt tcggtaaaag taaagatttc
    10801 tatatttatc agtatttttt caaaaaacag cagttataga ttgacaaagc ctctcgagta
    10861 tcgtaaactt tttttgtata aataactgaa tacgagatgc ctcaatcaat gaggtagagg
    10921 tcgcgagaat cattaaccct gtggagtgaa gcaaacatgg agaagcagga atagggagga
    10981 tcgccgaaat gtgcactttt ttgcttggag tgtatgttgg gacgcaagtt aagagcttgc
    11041 ggactgtctt agcagtgatg ctaagttgcg ctatgttttg tgagaggttt atcaccttat
    11101 ggaattttag cggccttttt atatctataa atcggtcgct ttttattttt tcagataaag
    11161 ctttaccgca tttcatctag accgctcgtg tatcatataa tcagaaaggg agaaaaaaat
    11221 gaaaaaaaat atggtttcag ttgccgtact atttgctttt gtcttctcac tattcagtgt
    11281 gggatctctg gcaaatgcag atgaaaaaac accgagcgat gagtttcgcg taggtatgga
    11341 ggctgggtat gcacccttca actggtctca acaaacagat gagaataatg ccttgccaat
    11401 tcaaggccaa aatagttacg ccggcggtta tgatgttcaa atcgctaaaa aagtagcaga
    11461 tggtttaggg aaaaaattag tcatcgtcca aacgaaatgg gatggtctgg ctcctgccct
    11521 tcaatcaggc aagatcgacg cgatcatcgc cggaatgagc cctactgctg aacgacgaaa
    11581 agaaattgat tttaccgatc cttattacga atcacaatta gtagtagtag ttcaaaaaga
    11641 tagcaaatat gcaaaagcaa aaagtttagc agatctttct ggagcaaaga ttactgctca
    11701 gttaaataca ttccactata ctgtgatcga tcagatacca gatgtgcaga aacaacaagc
    11761 aatggataat ttctcagcaa tgagaacagc tttagcttct ggaatgatcg atggttatgt
    11821 cagcgaacgt ccggaaggtg taacggcaac tagtgtgaac aaagacttgg aaatgcttga
    11881 attcagcggt gcagatggct tccagacaaa cccggaagat gttcagattt ctgttggtat
    11941 gcgtaaaggc gacccggata tccaaaaagt aaaccagatt ctttctggca tctctaacga
    12001 tgaacgaacc aaaattatgg accaagcagt caaagatcag cctgctgcaa ctgatagtga
    12061 agaaaaacct ggcatcattt ctgatttcac tacgatttgg aaacaatacg gcaatatgtt
    12121 cttacgaggt gctggactta ccttgttcat cgcattgatc gggacagttg tcggtactac
    12181 acttggatta ctgattggtg tcttccgttc ggtacctgaa tcaggaaatg cactgactcg
    12241 cttcttccaa aaaatcggaa atgcacttct ttctatctat gtcgaagtat tccgcggaac
    12301 accgatgatg gtacaagcaa tggtcatctt ctacggtctg gcactcgctt ttggcatttc
    12361 attgaaccga acgattgccg ctttgtttat tgtctctctc aatactggag cttacatgac
    12421 agagatcgtt cgtgggggga tttttgcagt agatgaaggg cagttcgaag ctgcgcaagc
    12481 cattggtatg acccatggcc aaacgatgag aaaagttgtt attccacaag ttttacggaa
    12541 tatcttgcca gcaacaggaa atgagtttgt tatcaatatc aaagatacag cagttttaag
    12601 tgtaatcggc gtagctgatt tgttcttcca aggaaactct gcttccggtg cgaatttcca
    12661 attcttccaa acatttacga tcgtcgggat catctactta gtcatgacct ttacgatcac
    12721 cagaatcctg cgggcggtag aaaagaaaat cgatggtcct tcagcttata ccaagcttga
    12781 agaaatcgac aattcaaatt tgcaggaata aggaggaaga atcatgaatg aaaaaattat
    12841 tgaaatcaaa catctaagaa aaagcttcgg tgaaaatgaa gtattgaaag atatcagcgt
    12901 aacagtcaat aaaggtgaag ttgtgacgat catcggttct tccggttctg gtaaatctac
    12961 tttcttgcgc tgtatcaatt tactggaaaa accaacaggc ggcgagatca tttataatgg
    13021 agaaaatgta ttagctccta aatataattt acct
//
LOCUS       DAXWLB010000069        12967 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXWLB010000069 DAXWLB010000000
VERSION     DAXWLB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.069"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            28..729
                     /locus_tag="ACGE4H_001550"
     CDS             28..729
                     /locus_tag="ACGE4H_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFF8364967.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            726..1847
                     /locus_tag="ACGE4H_001551"
     CDS             726..1847
                     /locus_tag="ACGE4H_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFF8364968.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1862..3094
                     /gene="pepT"
                     /locus_tag="ACGE4H_001552"
     CDS             1862..3094
                     /gene="pepT"
                     /locus_tag="ACGE4H_001552"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFF8364969.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3226..4134
                     /locus_tag="ACGE4H_001553"
     CDS             3226..4134
                     /locus_tag="ACGE4H_001553"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8364970.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4169..4441
                     /locus_tag="ACGE4H_001554"
     CDS             4169..4441
                     /locus_tag="ACGE4H_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFF8364971.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4452..5498
                     /locus_tag="ACGE4H_001555"
     CDS             4452..5498
                     /locus_tag="ACGE4H_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFF8364972.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5749..8358
                     /gene="clpB"
                     /locus_tag="ACGE4H_001556"
     CDS             5749..8358
                     /gene="clpB"
                     /locus_tag="ACGE4H_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFF8364973.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8509..9195)
                     /locus_tag="ACGE4H_001557"
     CDS             complement(8509..9195)
                     /locus_tag="ACGE4H_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8364974.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9173..9667)
                     /locus_tag="ACGE4H_001558"
     CDS             complement(9173..9667)
                     /locus_tag="ACGE4H_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8364975.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9687..10907)
                     /locus_tag="ACGE4H_001559"
     CDS             complement(9687..10907)
                     /locus_tag="ACGE4H_001559"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8364976.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11054..12889
                     /gene="lepA"
                     /locus_tag="ACGE4H_001560"
     CDS             11054..12889
                     /gene="lepA"
                     /locus_tag="ACGE4H_001560"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFF8364977.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4249 a         2372 c         2724 g         3622 t
ORIGIN      
        1 ctataattat ctagaaggag tgaagctatg aaccatttag atttatctca gcgcttagcc
       61 gcagtgggtg actttgtccc tgaatccgct cgtttagcag acattggatc agatcacgct
      121 tacttacccg ttgcattgat attaagagga aaaatcgaat atgcagttgc tggtgaagtc
      181 gttcaaggtc cttatgaatc agcacgtaag caagtcaaaa aagatggatt agaagaccgt
      241 atcaccgtac gccttgcaaa tggtctagat gctatccagc cacaagatgg tattacagct
      301 gtcacgattg cgggtatggg aggttcactg atccgagata tcctagaagc tggcaagttg
      361 aatcaacgct taagcggaca agaacgtttg atccttcagc caaacatcgg agagcgtaca
      421 ttacgatctt ggctgcaaga aaatcagtat aaaatccaat cagaaaaaat acttgaagaa
      481 aataaaaaaa tatatgaaat cattgtagcc gaaaaagaac aaaaaccagt tacttacaat
      541 gaaatggagc tgatgttcgg gccatttctg cttgaagaga aaagtgagat cttcaaatca
      601 aaatggacaa gagagcttca acaaagaaaa gctatccgtg aacagctcaa acaagcagct
      661 aatcaagagg cacgtcaaaa acaaatcgag caggaaatcc aaatgattga ggaggtttta
      721 aagccatgac aatcagcgga cgcactttca tccaaaagtt tgaaagctat tgtcctcagt
      781 ggctagctga agaaggagac ccagtcggac tgcatatcgg aacattggat aagccaatcc
      841 aacgagtaat gatgacatta gacgttcgtc cagaagtggt cgaagaagca atcaagaaaa
      901 aaatcgattt gctgattgct aaacatcctc caatctttcg tcctgtaaaa cggctggtta
      961 ctgatcagcc acaagaaaaa atgtatgcag atttgttgaa acatgatatt gccgtctatg
     1021 cagcacacac gaacatggat attatttggg atggactgaa tgactggttt tgtgaacttc
     1081 tcggtattga agtggagagt tatttagtaa agactcatga gattcattat aaaaaactgg
     1141 ccgtttatgt acctgtggat catgcacaga aaatgcgtga agtattagct gcagctggtg
     1201 ctggtacaca aggtgattat accggtacaa gttttacctc gattggacac ggtcgattta
     1261 ctccggaagc tggtgcacaa ccagcgattg ggaaagtagg gaagacagaa caagttcaag
     1321 aggcaaaagt tgaggttatt ttgccggaaa ctatcgaaaa acaagtgatt caagccatgc
     1381 ggtcagctca tccttatgag gaacctgctt atgatctttt cgctatagat gaaccagtcg
     1441 aaatgtttgg gttaggacga gtaggtgaat tgccgcaaga aatttctata gaggcatttg
     1501 tagaacaagt aaaagaagcg ttccaactag atggactgag aatagtccag ccaaaaaatg
     1561 caaaatcctc tgtgaagcgc attgcgattt gcggtggaag cggtgaaaaa ttttatccgc
     1621 aagcaatcgc ccaaagagca gatgtctata tcacgggaga tatctattat catacggctc
     1681 atgatatgca aagcgctggg ttgattgcaa ttgatcccgg tcattatatt gaatcgttat
     1741 gcaagcaacg atttatcgag aaatttgaat cttggaaaca agaagaaaat tgggatatta
     1801 actttttcgt ttctgaaaca aatacaaacc catttcaatt taattaaaag gagagaatac
     1861 tatgtacgaa aatcttttgc cacgtttttt acgatatgtc aaaacagaaa cacgctccga
     1921 tgcaacaagc aaaacgactc catcgactca gacacaagtt gcttttgcgc aagaattgaa
     1981 aaaagaatta gaagagttag gcttagctga tgttttctat aatgaagata acggattcgt
     2041 tatgggaaca ttaccaagca atgttgatca tgaagtgcgt tccattggat ttatttccca
     2101 catggataca gcagatttta atgcagtagg agtcaatcca caaatcattg aaaactatga
     2161 tggcgagtct acgatcgtac tagatgcgga agaacgctat acattgaata caaaagattt
     2221 tccaaatttg aaaaattatg aaggccacac attgatcaca acagatggca gtacattact
     2281 gggagcagat gacaaatcag ggattgcgga aatcatgact gcaatggaga tcttgatcaa
     2341 taatccttct atccctcacg gtaccatcaa agtggctttt ggtcctgatg aagaaatcgg
     2401 tgtcggagcg gataaattcg atgttgagca attcgatgta gattttgctt atacagtcga
     2461 tggtggtcct ttaggtgagt tgcagtatga aacgtttagt gctgctcaag caaatattac
     2521 gatccaaggg aaaaatgtac atccagggac ggcaaaagat acaatgatca atgctctaca
     2581 gttagcaatc gattttcata atcagcttcc agccgatgaa gttccagaaa aaacggaagg
     2641 atacgaaggt ttcttccatt tgatggcatt ggatgggaat cctgaagaag cttctatgag
     2701 ttatatcatt cgcgatcatg atcgtgaaaa attcgaagca cgcaaagcac aaatcacaca
     2761 tatccaagaa gagttgaatc agcgatttga tcaagaacga atcaaagtag atatgtatga
     2821 tcaatattac aacatgaaag aaatcattga aaaagatatg agcatcgtgg aattagcgaa
     2881 acaagcaatg atcgagcttg gtatctctcc agtgatcgaa ccagttcgtg gaggaacaga
     2941 tggttcaaaa atctcttaca tgggtattcc gacgccaaat ctatttgccg gaggagaaaa
     3001 tatgcacgga cgattcgaat ttgtttcttt acaagtaatg gaaaaagcaa cagacgtgat
     3061 tgtaaaaatt gcagaattga acacaaagag ttaaaatcga aaaagaacag cttatgacaa
     3121 ggcgttcgtc ccaatgatgg aatgtcatct gtctaaggct aaagggcagg acaatggaat
     3181 tggttgtccc gccctctttt ttgaagagta aataggaggt aaagaatgga aaagctgcta
     3241 gttttttata acaaaagttc agggaaagat gagggtgaga aattagcacg ctggtttcaa
     3301 gattatgtaa aagaaaagca gccagaatta tatgtagaac tagctgaaac tggcccatcg
     3361 attgatgatg cggaattagt caagaaagca gaagccatag aagctgatac tttagtaatt
     3421 ataggtggag acggcacgat tcatcatatc gtccaagcgt tccaaaaaac attatctcaa
     3481 taccatgtcg gattacttcc aggtggaact gtcaacaacc ttgcacgtgt tcttgagatt
     3541 cccctcaaaa aagaagaagc tgctgacgtg atcctggaag gcaatgtacg gaaaatagac
     3601 tatggtcaag tcaatgatga agtcattatc tctacactga caatcggaat cttagcagat
     3661 acagcagttt ggatcagtca agaagaaaaa cagcgttacg gctcgtggat ctttatcaag
     3721 aagtttttta agttattgct gaagaagaaa aaatatcatt tggatttaga aacagacgag
     3781 gagcattgga aaggaaaatc ccaactgttg acagtcacga tgtctaactc cgtgggcggt
     3841 tttacaaatt ttgacgactc agcgacccta gatgatggaa aatttcatgt aacgatcgta
     3901 ccttccttag atattttccg atttattttt tatttgccta gaatcatgag aggcaaattc
     3961 tatacgatcc ccggcattac ttatttcaca gccaaacgca tgaaaatcca agcgcaggaa
     4021 aaatctgtgg gcactcgaac ggatggagac acgacagatg atcttccagt tgaattatct
     4081 gttgtatcaa aaggattaac agtttattca ccaaagaaag aagaaaaagc ttagaaatag
     4141 tagttaacag aaagttggaa gaagagtaat ggatgtattg ttacacaatc ctttgtggtt
     4201 ttaccttttc attgtgaatt tttacttatt ttgtctgatg gggtatgata aacatcaggc
     4261 tagaactagc ggctggcgtg ttcctgagaa taatttgata tttgtcagcc tgttaggcgg
     4321 tggtttggga ggattgatct ctcgaaaagt tttccatcat aaaactcgga aaaagaagtt
     4381 tactatagga ttcgctgcag gtgtacttgt tgatttgatt ctcatttatt ttttccatta
     4441 aggagcagga gatgtcgtct aaaggaacga aaactaaaat cattctgaat ctgcttttgc
     4501 tgattgtgat ttttgccatt atttattatt taatcaatca atcatttgca gatatctttg
     4561 ccgaattgct gtctaccagt gttgttgttt tggcagcggc tgttatatta gggacagttt
     4621 accaaatcgc tgaagggcgc tcaatcaaag aaattgctga acctttcgcg ccgaatttta
     4681 caacgaaaga tgggttcttg acctctagct atattgcgtt ttaccgaatc gtttcgtttg
     4741 gtacaggaac gctgatctcg gagatttatt tttataacaa aaaaggaatg aagctatccc
     4801 agggagcagg ggtgacagca ctccatatga ttatgtacaa actggcagtg atcacttatg
     4861 cattagctgg cttggtcatc caattctctc ttttttattc taatggtcca tcaatgatct
     4921 ggtttatcat cgctggtatc cttttgacga tcgtgattgt tgccttttta ttggcgatgt
     4981 ccgtcagctt gaatgttcaa atattttttg tcagcttatc gaataagtta tttaaaagtg
     5041 agcgtttgcg gaaatgggtg gatacatgca atcagcagat ctactcttta agggaaacgg
     5101 tccaaaccat tatccaagac aggactgcgc tattgcggat ttatctatgg aatatgttaa
     5161 aacttgtatt ttggtatgta cttccttatt tgattctgat tgaaaaccat cctaaaatag
     5221 attttctact gaccatttcg tttatcagct ttgcagtagt gctttcaggg gttattccta
     5281 cgcctgctgg tattggttcc tttgaatttg tctatttgtt attattcaga ccagttgttg
     5341 gaacagttga cgcagtttct tcgcttttac tgtatcgttt cagttccttt atcttgccat
     5401 ttctctatgg atttgtgtat gtgattgctg aacggcgaag agtgatcaaa gaagaaataa
     5461 tagaagtaaa aaaagagaat aaagaaaatc aagggtaggt cttttataag atctaccctt
     5521 tttatcaaat caaagaaaaa cgttatggat aagataattt tacgtttgtt tggctctttt
     5581 tttatataca tacaatcaat tttgtttttt taagatttat ataagtcgag aaaaaaagtc
     5641 aaaaaactat gtgtatattt tgaccaaaat tgaccaaagc gatataatag tttttgtaaa
     5701 gatcaaagaa ataagtgatc ttacctttaa ttaggagggt atacctgtat gaatattgag
     5761 aaaatgacta cgacgcttca agaagcgatt gctgaagctc aacagattgc ggtcactcga
     5821 aaacatcaag atatcgacat tgcccatgta tggaaaatct ttttacaatc taatcatttt
     5881 ggtcgaaatt tctatacgga tgccggattg gatgtcgaat cttttgaaca tgaaattgat
     5941 cgtctactgg atgagtatcc agttgtttct ggtggaaacg tccaatatgg acaaaacctc
     6001 agccagaatt tctttcgatt attgaatgaa gcagaccaaa tacgtgaaag cttcggcgac
     6061 gagtttcttt caacggaagt cgttatttta gcgttgatga aattaaaaaa ttatccgtta
     6121 actgtttatt tgaataaaaa cggattatct gaaaaagaat tgcgtaaaaa catcgaagag
     6181 atgagaggag gagaaagagt gacctcacaa aaccaagaag aacaatacaa agcattagaa
     6241 aaatacggcg ttgatttagt acagcaagtc aaaagcggaa aaatggatcc aatcatcggt
     6301 cgtgatgaag aaatccgtga tgtcatccgt attctttcac gaaaaacaaa aaataatcct
     6361 gttctgatag gagaacccgg tgtagggaaa acggcaatcg ttgaagggct tgctcaacgt
     6421 atcgtacgca aagatgtgcc tgaaaattta aaagataaaa cgattttttc attagatatg
     6481 ggtgcattga tcgctggagc aaaattccgt ggtgaatttg aagaaagact aaaagcagtc
     6541 ttgaaagaag tcaaaaaaag tgatggacgt atcctcttgt tcatcgatga aatccacaat
     6601 atcgtagggg caggaaaaac agaaggaagt atggatgccg gaaatctgtt gaaaccaatg
     6661 cttgctcgcg gtgaattaca tttgatcggt gccacaactt tagatgagta ccgtcaatat
     6721 atggaaaaag acaaagcgtt ggaacgtcgc tttcaaaagg tattagtcaa agaaccaaca
     6781 gtggaagata cgatcagtat tctgcgtgga ttaaaagaac gttttgagat ccaccatggt
     6841 gttaacatcc atgacaacgc attagtagca gcagctactt tatctgaccg ttatattact
     6901 gatcggttct taccggacaa agcaatcgat ttgatcgatg aagccagtgc aacgattcgg
     6961 gtagaaatga actctatgcc aacggaatta gatcaagtta ctagacgttt gatgcagttg
     7021 gaaatcgaag aagctgcact gaaaaaagaa tcagatgatg caagtaaaaa acgcttgaaa
     7081 aatttgcaag aagaattagc tgaacttcgt gaagaagcaa atgcgatgaa gatgcagtgg
     7141 gaaacagaaa aagaagaagt caattctgtt tcagcaaaac gtgctgaaat cgataaagca
     7201 aaacacgaat tagaagatgc agaaaataac tatgatttag aacgcgctgc tgtattgcgt
     7261 catggtacga tacctcaatt agaaaaagaa ttgaaggaat tagaagctaa agcaaaagac
     7321 agcgagatta agatggttca agagtccgtt accgaaaatg agattgctca agtcgttggc
     7381 agattaacgg gtatccccgt aacgaaactt gtagaaggtg aacgagaaaa actaattaaa
     7441 ttgaatgaaa cacttcataa acgagtgatt ggtcaagatg aagcagttga tgcggtcagt
     7501 gacgcggtaa ttcgttcacg ggctggattg caagacccaa atcgtcctct aggttctttc
     7561 cttttcctag gaccaactgg tgtcggaaaa accgaattag cgaaagcgtt agctgagaac
     7621 ttgtttgact cagaagatca tatggtacgg atcgatatga gtgaatatat ggaaaaacat
     7681 gctgtgtctc gtctagtcgg agctcctcca ggctatgtag gttacgaaga aggtggtcaa
     7741 ttaacggaag ctgtcagacg aaatccttat acaatcgtct tgttggatga aatcgaaaaa
     7801 gcgcatcccg atgtcttcaa tatcttgtta caagtcctag atgacggacg cttgactgat
     7861 tcaaaagggc gagtagtaga tttcaaaaat acggttctga tcatgaccag caatatcggt
     7921 tcacagttac tgctggaagg cgtgactgct gatggaacga ttccagaagc agtggctgaa
     7981 caagtcaaca cgctattacg tggaaacttc aaaccagaat tcttgaaccg aatcgacgac
     8041 acgattttat ttacaccatt gagcctagac aacgtgaaag gaatcgttga caaaatggtg
     8101 gcgcaattgg cacaacgtct ggaacatcaa gaaatcctgc tgacaatcag tgatgaagcc
     8161 aaaacgtgga ttgctgaaaa tgcttatgaa ccagcatatg gtgcaagacc attgaaacga
     8221 tttatcacta aggaagtaga aacaccacta gctaaagaaa tcgttgcagg acatgttatg
     8281 cctaaatcaa aagtaacgat tactttactt gatggacaac ttcatttcaa aacagaagag
     8341 ctagaagaaa tcgtctaatc ttccattgag actataaaag aaaaaagggg tctgacacaa
     8401 ctagtgtcag acctcctttt ttcaaataaa cagtgttcca gaagtagttt cttcgaaaaa
     8461 taagccgaaa tttcttgaag ctgcctgctt ctgtcacaac ctcttttatt aggtattttt
     8521 cactttttct aatttataac tttcaaagta acgctcatct tttttacttt gagaccatgc
     8581 aaacaatccc attaaggcag cacctatcgt atttgtcagc aagtcgccca tcgtatccat
     8641 caatgctgct cgtccgacaa acagtgtgcc atcagaagca gcaaaacgtt gaagattcat
     8701 gcctaagaac tggtcgcata agaattccca gaactcccaa aacacgccgc aaagtccagc
     8761 aaacgcaaag ccgaataaga ggaacaacca aggactggtt tttgatatct cagcatcttt
     8821 catcaagtaa ccaatcaatc catagccaag agctgtcagg accattggac tcaccgcatg
     8881 taagattttg tcccaaaaag agatgatact gatcaaatgc atccctgtgc ctaaaaagac
     8941 agagatgaac aagaaaaacc agtaaaacaa aacgattgct gctggtattt taagtttgaa
     9001 aactttcaaa atgacttgtg gcatgaaaat caatgcaatc cctaataaag attccacgat
     9061 aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata aattcataaa aattatagca
     9121 gacaacgaat acaccaaaag caagtaaagc tatttgcacc tttttgattt ctttaatcac
     9181 tgaaagtccc tccatctaaa aatcttttta cttctgaaag caactcaatt tctgaagaaa
     9241 ccacttttcc gttcaatttg atcaaaccta ttgtatataa gttcaggtag tgaaattgat
     9301 tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg ggttccgcct tgctgtcgag
     9361 tatcagtata taagccgatc actgggatgc ctttcgcata agccacaccg atttccgatg
     9421 ctaccccggc atcgatcgtc agcccatcta gtaaagcaat cattaaatca ctagccaaca
     9481 ctttttctgt gtcagcaaga gcaatcattc gactgtctgc ataagcagtt ttgtcattga
     9541 ttgcttcatt ttcttgagga agatagatcg aaagctcaga agagagctct ctgatttctt
     9601 taactaactg ttggttatat aacaaatcac ttttagcaaa cattggtgcg gcaaaataaa
     9661 ttttcatcta aacatcatcc tttttatcaa aaataatcgc taaagtaagc ttccatgttt
     9721 ggaatgatac gctctagtga cttcagtgtt tcttcatctg tttctaaccg ttcgattctt
     9781 gccaaataag aagcacggcg ataattcatc atcaagcaag tcaatgtttg gatatccagt
     9841 cgaacggatt ttcctatagg ttcatttaag acacggactt gtccctgttc atcccatgtt
     9901 aaagcgaaga caccattatt ccaaggagcg acaggatctt caatgatgaa atgaaaagca
     9961 tcagctgttc caacaaacgg aaaccgttga aggaattctt ttacatcaac gatccgagcc
    10021 ataaaatatg gttcgatctg ctctttgatc tgactatctt caatcaaaaa tgctaaaggc
    10081 tcatttttat aaatatctcc atgtacccag tagatcatgg aaaaatgtgc ggaaataaag
    10141 ttccataagc catttcgagc ttcttgatca agatagaaca tttcttttac gtgaaatatt
    10201 tcttcagcta cccagtagaa cagaacacct cttggttcat ggttgctatc gtagtagaca
    10261 gcggctgtgc gctcatcttc attttcgaaa cgccaatact cttcccaatg aaaatcactg
    10321 cggaataaag caccatggtt ttgttgggca aatttgtcat aaacggaaaa tacgtctgga
    10381 tgatcaactg gtaatcgctc aaccatacca cttactggga tttgtttagg tagctgtgta
    10441 tctctgattt taaaggaaag tttatcagac atgatttccc atcctttacg ccgatagtag
    10501 gggatactgt aaggatacaa ataagagatc cattgcttat tcttgcgcat attatccaat
    10561 gcaacgataa ttagatcttg catcaatcca tggttagcat attctggata agttccaact
    10621 ccagtcacac cacccatttt ataacgtgca ccgtgaatat tgacttcaca aggatagata
    10681 gcgatttgag agatcagttt attttcgtgg aaccatccga acactttcga tacttccaaa
    10741 atcggttttt tggaacggat aaaagctcgt ttattctcaa acccgctttc ctcgatgtct
    10801 gcttcagtca cttggaacac ataggaaagc aattcattga attgatcgat atgttcttga
    10861 gtgaccggtt ttaattcaag attttctcta aaatcttttt gatccatttt ttcatctcca
    10921 tttattagct tgataacagt atagcaaaat tctacaaaaa aaacttctat agctgactaa
    10981 ttaaaaactc tttaaattac tgcttttcgt tggtataatg gaaagtgctg attatatgaa
    11041 aagaggagac gctatggata taaagaaaat gaaagaacgc caagagcaga ttcgtaattt
    11101 ttcgatcatc gctcatatcg accatgggaa atctacattg gccgatcgta ttttagaaat
    11161 gacgcacact gtgacgtctc gcgaaatgca agatcagctg ttagactcga tggatttgga
    11221 acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa ctcaattaca cagcgaaaaa
    11281 tggagaaaca tatatctttc acttgattga caccccaggg cacgtcgact tcacctatga
    11341 agtctctcga agccttgctg cctgtgaggg tgccgtttta gtggtcgatg cagcacaagg
    11401 aatcgaagca caaacattgg ccaatgttta tttagcgtta gacaatgatt tggagatcct
    11461 gccagtcatc aataaaatcg acctacccgc agcagatccc gaacgtgtcc gtcaagaaat
    11521 cgaagacgta atcggaatcg atgctagtga agctgtatta gccagtgcca aatcagggat
    11581 tgggattgaa gatattctcg agcagatcgt ggaatatgtt cctgcaccaa gtggagactt
    11641 agatgcaccg ctaaaagctt tgatctttga ctctatttat gatagttacc gcggtgttgt
    11701 tctaaatgtc cggatcacgg acggtatggt caaacccgga gacaaaatca aactgatgag
    11761 taatggcaaa acatttgatg ttacagaggt cggggtcttc tcgccaaaag cagttgctag
    11821 agactttttg atggtcggtg atgtcggtta tatcacagca agtatcaaaa ctgtccaaga
    11881 tacacgagta ggggatacag taacgttagc agacaatcct gccgctgaag ctttaccagg
    11941 ttaccgaaaa atgaatccga tggtttattg tggtctttat ccaattgata cttctcgtta
    12001 caacgatttg cgggaagctt tagaaaaact gcaattgaat gatgctgctt tacaattcga
    12061 accagaaacc tctcaagcat taggttttgg gttccgctgt ggattcctcg gacttcttca
    12121 tatggatgtc gtccaagaac gcttggaacg ggaattcaat ctagaattga tcacgactgc
    12181 accatccgtt atttaccatg ttaataaaac ggatggtacg actgctacag tagataaccc
    12241 ggcagatttt cctgaacctg taacgattca ggatgtagaa gaaccattcg tcaaagcaca
    12301 gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa ctttctcaac gaaaacgggg
    12361 agaattcatt acgatggatt atttagatga ttaccgagta aatgtcgtct ataatattcc
    12421 gctttctgaa attgtctttg actttttcga caaattgaaa tcaagcacga aaggctatgc
    12481 ctcattggat tatgaaatgt ctggttatca aaaaagcaag ttagtgaaga tggacatctt
    12541 gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt caccgagact ttgcctatga
    12601 acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc ccacgccaac aatttgaggt
    12661 gccaatccaa gcagctattg gacaaaaaat cgttgcccgt tcagacatca aagccttgcg
    12721 taaaaacgtc ttggctaaat gttatggtgg agatgtttct cgtaaacgta aactcttaga
    12781 gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc tccgtcgaag ttccgcaaga
    12841 agccttcatg gccgttctga aaatggacga ggacgaaccg aagaaatagc aagggtttgt
    12901 ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt tgccaccggt ttgccaccgg
    12961 tggtttt
//
LOCUS       DAXWLB010000070        12912 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXWLB010000070 DAXWLB010000000
VERSION     DAXWLB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.070"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            126..1118
                     /locus_tag="ACGE4H_001561"
     CDS             126..1118
                     /locus_tag="ACGE4H_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8364978.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1178..2599
                     /locus_tag="ACGE4H_001562"
     CDS             1178..2599
                     /locus_tag="ACGE4H_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFF8364979.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2592..4091
                     /locus_tag="ACGE4H_001563"
     CDS             2592..4091
                     /locus_tag="ACGE4H_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFF8364980.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4130..5524
                     /gene="uxaC"
                     /locus_tag="ACGE4H_001564"
     CDS             4130..5524
                     /gene="uxaC"
                     /locus_tag="ACGE4H_001564"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFF8364981.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            5683..6366
                     /locus_tag="ACGE4H_001565"
     CDS             5683..6366
                     /locus_tag="ACGE4H_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFF8364982.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            6368..7204
                     /locus_tag="ACGE4H_001566"
     CDS             6368..7204
                     /locus_tag="ACGE4H_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFF8364983.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            7215..8969
                     /locus_tag="ACGE4H_001567"
     CDS             7215..8969
                     /locus_tag="ACGE4H_001567"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8364984.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(9245..9586)
                     /locus_tag="ACGE4H_001568"
     CDS             complement(9245..9586)
                     /locus_tag="ACGE4H_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFF8364985.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(9654..11825)
                     /locus_tag="ACGE4H_001569"
     CDS             complement(9654..11825)
                     /locus_tag="ACGE4H_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFF8364986.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            12036..12893
                     /locus_tag="ACGE4H_001570"
     CDS             12036..12893
                     /locus_tag="ACGE4H_001570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFF8364987.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
BASE COUNT         3950 a         2575 c         2529 g         3858 t
ORIGIN      
        1 aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga tctattgact
       61 tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg
      121 atggaatggt aacgatcaaa gatgtcgcta aagcagctgg cgtttctcat acgacagttt
      181 ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa atacgacaac
      241 tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa
      301 aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca aatttcctag
      361 cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta tctgttgaaa
      421 gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga ctgatcgtta
      481 tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa gaatttcctt
      541 tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact gccgatgatg
      601 cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg aagatcgctt
      661 atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa
      721 gtctaaaaaa agcgagcatc cctgtcaatc cagcctattt tttaacagga gattacagct
      781 tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg cctactctgg
      841 ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat ttagctggcc
      901 tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc gtctcctatc
      961 tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa
     1021 agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt gagctctcca
     1081 ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat tttatataaa
     1141 aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga caaaagaatt
     1201 agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg
     1261 tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta aaggtaatgt
     1321 tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc aagataattt
     1381 atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt ccgagattat
     1441 caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc tatctctagc
     1501 agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa tccaattcgt
     1561 ggagactgat aatttaacag atactccccc acagagcctc ccgggcaaac tgacagcctt
     1621 tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt gcgaactgat
     1681 tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa aatgggattt
     1741 agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta gtttagttga
     1801 tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta
     1861 tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg aagggcctaa
     1921 agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca tcacagatga
     1981 tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata cagcgatggt
     2041 ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag atcctttatt
     2101 ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt ctttacctaa
     2161 agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc cttttgccca
     2221 tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc
     2281 agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca ctgtttcact
     2341 agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg atgaaaagac
     2401 gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc gattattgca
     2461 aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag atacagttca
     2521 agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag aaatgaaagg
     2581 aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga cgagacagtg
     2641 tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat cagcaaacga
     2701 tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat ttgaaagctg
     2761 gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac gtaactgccg
     2821 gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg gattatcgat
     2881 atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt caaggttact
     2941 tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct accgttgggt
     3001 gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg
     3061 gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag cttggaaaag
     3121 atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat gccggaggag
     3181 ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa aaaattttag
     3241 gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac gatgagatcg
     3301 aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac cagcgcatat
     3361 cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg
     3421 gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct caaggaggca
     3481 gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg atggcacgag
     3541 cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc aaacagtatt
     3601 tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa gcaggtggga
     3661 tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg
     3721 ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc
     3781 ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag ttggtcttgt
     3841 ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag gtatctacga
     3901 atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc gtcttattag
     3961 aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg
     4021 gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt aaaaacggag
     4081 taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta tgtttttatc
     4141 tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct acgcaaaaaa
     4201 catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg aaaacaacaa
     4261 tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc gcttgatgcg
     4321 agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg aaaaattttt
     4381 agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt ggtcacatct
     4441 tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg cagagaaaat
     4501 ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa
     4561 aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact tgcactatca
     4621 tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc gtccagataa
     4681 agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg aagaagcagc
     4741 tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc ggatagaata
     4801 cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac gctataaaga
     4861 agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca aactattatc
     4921 ttcagatgaa atcgatgctt atcgtacaga aacactgata catttgaccc acttctatca
     4981 ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta atacacaaat
     5041 gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt ctttgactat
     5101 tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa agacgatcct
     5161 ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct gtttccaaaa
     5221 agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata ctcgagctgg
     5281 tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt ttgtcggcat
     5341 gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc gtcgtgttct
     5401 ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg aacaactttt
     5461 aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg gcttttttgc
     5521 ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata acggaatccg
     5581 tttatccacg ttttagagaa gaagacggac caaatttgct ccgtcttctt ctcttgctgt
     5641 tctgcttcat tgcctataaa cctttatgaa agaagagata acatgcctac tatccatatt
     5701 gccggtgatt ctactgcggc agaaaaagat gaaaacaagc gtcccgagac aggttgggga
     5761 gaaaaaatct ctgcttattt ttcacccgag atccagatca ataaccaagc aaagaatgga
     5821 cgcagcacca aatcatttat ccaggaagga aggttagccc gtctagcttc acagtttcag
     5881 ccgaatgatt tcttatttgt acaatttgga cataacgacc agaaaatcca agaagaaaga
     5941 ggtacgcttc ccttcggtag ctatttaaaa aatctgcaga tattttttga atgcgcaaaa
     6001 aaagcgaacg tccaaatggt cttactgaca tctgtgacgc gacgagatta cttagaaaac
     6061 ggaacattga atccagatat acttggagac tatccaaaag cgatgcgtgc atttgctgag
     6121 aaacaccata ttcctctttt agatgtgttc tcaagatcac aagaattgtt ccagaccttc
     6181 tcaaaagaag agacaaaaaa attctacctg catcttatac ctgatacttg taaaaattac
     6241 ccagaaggac taaaagacaa tacccacttt agtccagaag gcgctgataa agttgcccga
     6301 ataattgttg aattgataaa agaatcccgt ctgcctcttg ctaattattt acaaaaagga
     6361 gtttgagatg tttccattaa atttaggcat ccgtgcccac gatttagatg ttcgaaatcg
     6421 tgaagaaatc gttcaaaaag tatcacagct tcacttgtcc catatccaat ttgcaccaca
     6481 aaaagcattt cctgaacaac ttaaattaac tgacatgaca cttgggagtg ctgcctattt
     6541 tggtgaatac ttccaaaaaa acaatatcga actttccatt ttaggatgct atatcaacct
     6601 atccagcaaa aatcaagaag ttcgtgcaca agcattggca actttcaaac agcaaattgc
     6661 tctgtgctct gcctatcaag ccaagatgat tgctacggaa accggtagtg tctctgtcgg
     6721 ttacacaaaa gaaaatttta ctgaagaagc ttatcagatc gccagaaatt ctatcattga
     6781 attagtcgct catgcagaaa attttggcgt aactgttgct gttgaagcag ggatcaatca
     6841 tccaatctat aactatcagt tagctaaacg tttaatcgat gaagtagctt caccaaattt
     6901 aaaaatcatt ttagattgtg cgaatttgat gcataaagaa aatcatgctg atcaggaaaa
     6961 agtgatccga ggtgccttgg acaatttaca tggttatatt acagcgcttc atctcaaaga
     7021 ctatatcatg gaaaatggaa agatccgcat ggtacctgtt ggcaagggct gtttagattt
     7081 ccgcccggta ctgcattata tcaaatatga gcgtcctcta atgtacgcca ctcttgaagc
     7141 tactcctgag atttatgttc ctgaagcaat agaacacttg cagtttattt accaagattg
     7201 ttaaggagaa gattatggag gaacgaatac agaaaagtta tcaaaaatat catgcaaatg
     7261 cttttcagac attgctgcat ctttatcagg gaaattattg gcgttttgcc atgtcaggac
     7321 tcttttttat tatcaaacat cttccatctt ggttaatgcc gatcgctatt gccaatgtaa
     7381 tcaatgcagc ttcatcgaaa gattcttctc agttgcaggc catttatttc aacgcttttt
     7441 tgatggcttg tctgattggt caaaatatat tgacgaatta ttttcatgta aaataccata
     7501 gcacttcgat ccgtcaagta gaagctggac taagagcgac ccttatcaag aaaatccaag
     7561 aactatctat tccttatcaa aaagaattgc aatctggacg aatccagtca aagatcatcc
     7621 gtgatgtaga agctatccaa acgttgtcct ctcaagtatt tgtcagcgga ttaaatatcg
     7681 ctgtcaattt attcgtagct ttaggtatca cgctttataa aagtcctgtt gttttcatgt
     7741 ttttcttatt gactgttcct ctcgccgcat tcgttactct ctttttccgt aaaaggatca
     7801 gacagtccaa ctcagacttc aggcaagaaa tggaagagac ttctgcaaaa gtgatcgaag
     7861 tgatcgaatt agcaccgatt gctcgagctc atgggttaga aaaacacgaa acaactcgct
     7921 taaataagca ttttactcaa atctatgaaa aaggattcca cttagatatc ctgcaatctg
     7981 ttttcggatc agtcagctgg gctagttttc agatatttca gctgatttgt ttgattttca
     8041 ctgggatatt agctttacaa gggcgcatcc aacccggcga tgtcgtgatg tatcagacgt
     8101 atttcacgac gatcgtcaat tccgtttctg gaatgatcac gctgattcct acgatttcaa
     8161 aaggcatgga atctatcact tctattggcg aactgttgat tgctggcgat gtagaaactt
     8221 atcacggtaa gaaaaaaatc caacatttgg atggaaattt tcgttttgag gatgtttctt
     8281 ttctttatcc ggactcactc aaaccgatta tcgatcattt ttctctagag gtgaaagctg
     8341 gagaaacaat tgcttttgtt gggccatctg gctctggaaa atctacactc cttaacttat
     8401 tgattggatt tatccagccg acttcaggaa aaatttttct agatgatcag cctttggatg
     8461 aactggatat gcgaagtgtg cgaaattatt tagccgttgt tcctcaaacc actttgttgt
     8521 tctccgcttc gatcaaagag aacatcactt atggcttgaa aaacgtatca aaagaacgtt
     8581 tagatgaagt catcgaagct gcgcaacttt cttcactgat tgaccagctt cctgatggtc
     8641 tggatacctt agtaggcgaa catggcaaca agctgtctgg tggccaaaag caacgtatct
     8701 ccattgcacg agcgttgata cgtcagccaa aaatcatttt gctagatgaa gcaacctcag
     8761 ctttagataa ccagtcagag aaaaaaatcc agcaagccct gaattacctg acagaaacac
     8821 ccactacttt tattgtggct catcggcttt caacgatcaa agaggcagat aaaatcgttg
     8881 tgatcgatga aggaaaaatc gttgaaatag gtacttacga aaaactgtta gctcaaaaag
     8941 gctatttcta ccgcttgaaa aatgaatgac aaaaaatagg agctatgaca agacttttgt
     9001 cgtagctcct atttctgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     9061 atagtcatca aacatgagaa gctgtttttt gaaattgttc cttgtaactc agagttaaac
     9121 ggtaggctca cgacctcttg gtaaatggtg ttcaagagac agcctatcgg caataagaag
     9181 caattttgtc atcgctcaag gccgaacgtc tctttcacac cctctgttgt ttcataaaat
     9241 agttttattc gctgtaaagg tcgcgaactg gtgtcatgat gatacggttc aagtcattga
     9301 tgattgtact gaagccttgc tctttttgca tcaagtcatt gattacttct tctgattgga
     9361 ttttcacagc catttcttgt gctttcattg catcttcgtc gctgaactct tgtccactca
     9421 tttgtttttc ttgtaattcg atttgcatcg cttggaagtc tttgaacagt ttgtgtgctt
     9481 cttcgtttgc ttgtactttc tcgtacgctt ctttcaacgc tttgaattct ggtaattcac
     9541 ggatctcacg ttcgatttga ttcgcgctat cgtagatgtt tgacatgtta tgccctcttt
     9601 tcttttagta gttactctct attgtaacat ggggttattc gttttcaaag gggctactct
     9661 ccttgatatt tccgccagag gtctttagcc gcttcaccaa ttccctgaac gccttctttc
     9721 accatatcgc cgaagttttt cagcccttct tttgcttgtt caccatattg attcaagtta
     9781 tcttgccaat ttccagactc tccattagaa tttcctgtgt ttccaacttg atctgcagct
     9841 accacttgtc ctcctgtggc ataggcatct gctacactaa atcctgtttt tgaagtatac
     9901 ggaaggatgc cttctgccgc tgatttgaag actttcccga caacatttcc acttgtccct
     9961 tccaaatagt gcagcttgct ggattcttcg taacccatcc atgtcgaaat cactacgtct
    10021 ggcgtgtaac cgatcatcca ttggtcattt gcttttgtgg catcgaaatt agtttctgtg
    10081 gttcctgttt tccctgctat cgtataacct gcaggattcg cagcagccgc tgttccattt
    10141 gaaaatgtac caagaagcat gctggtcatt tcatcggctg tttcctttga tatgacctgt
    10201 tttggttttt tgtttgtgtt atcgacgatc accgctccag tcgaatcaac gattttcgtg
    10261 ataagatgcg gcgtatacat ctgaccatca ttggcaaatg cactgtaagc acccgccatg
    10321 atcaatggag atactccttt ttccagtcca ccaagggcta agccataata cttatcagat
    10381 tttgctaaag gcaggccgaa ttcttctgct ttatcatatc ctttttgtaa accgatctca
    10441 tgcaaaagcc atacagtagg caaattcaaa ctttgtgcaa cagcttgata catcggtact
    10501 tctccttggt atgtcccgtc aaaattatgc gcatcgtaat aactttgcgg ttcgtctttc
    10561 aagatactgg aaggcttgta tccagcttcc agtgctggtg tatatacgct taatggtttg
    10621 atcgttgatc ctggtgaacg acgcatctgt gtagcaaagc tgaatcctct aaagacatga
    10681 tctcctcttc gtcctactag tgcttgtaca ccacctgtat tcggatctaa agctactgaa
    10741 gcagattgga ccatggcacc atctgaagca tttgctggga ataatgcact gttttgataa
    10801 gtagcttcta attgcttttg ataatcctga tctaaagctg tatagatttt atagccttta
    10861 ttcatgacat cttcttcatc aagaccatac tcgttaactg cttcatcgat cactgcatca
    10921 aaataataag gataacgata accagaatct gaattatcgt atgtgtcatt aagatacgcg
    10981 ctgatatctg tctggctttc tgcttgttct tgttcttttg tgatttttcc gttttcttcc
    11041 attacactta gtactgtgtt tttccgatca ttcgcatttt ttgggtgatc gatcggatta
    11101 taaataccag gacctttcag cattcctgcc aaagtagcag cctcagacag cgtaacattc
    11161 gttgcatcta ctccaaaata ctttctggaa gcgtcttgga caccccatac cccgttgcca
    11221 aaataggcat tgttaagata catggtcaag atttcttctt tgctgtactt cttttcgatc
    11281 tcgattgcca aaaataattc cttcgctttt cgatcaaacg tttggtctaa tgtcagatac
    11341 gcatttttgg caagctgctg agtgatcgta cttcctccgc cccctgatgt tccgccagac
    11401 aagaccattc ggactgctgc acgggcaatc cctttgatat caaacccgtg atgctcgtaa
    11461 aaattcctat cttctgtcga gattactgca tcttggatat agggagaaat cgcgtttaat
    11521 tctacatatg ttcctttttg ggcgtacagt gtgccagctt cttcgttttt cttatcataa
    11581 atgactgtcg attggcttaa tcctgatttt aacgtttcga catttgcttg ttttgctaaa
    11641 ataaagaggt agatacttgt cactaagaca cagatcaaac caatcagcaa aatcaatttg
    11701 ttcacatgat attttttcca gatgcgcttt cgccatcgat ggaattgtcc gagatatggt
    11761 ctgatccaac gccaaaaact gatcaaagca gcttttatct tttctaaaat gctttgaaaa
    11821 tccatttgct tcttctcctg tctatttgat tcaatcgtct tattttacca tagtgaaaga
    11881 gtgttttctt cctgcttaag ttgtagttca atatgttttt ttacgttagt gcaagtagag
    11941 taacataaga atgaacagaa aaatgtaagt tttttcttaa atatgtttta aaaatggtac
    12001 aattagtctg cgattgcact gaaaggagta cacttgtgaa aatcacgatc actttaccta
    12061 tgaaccatcc tgaaacaaca attcgcgact tgcttgaaaa ggaatggtta gttcctagaa
    12121 aggttcgaca ctttttgcgt atacgtaaaa acgtttggat caatcaacaa cctgctttgt
    12181 tccatcatga agtaaagggc aatgaccaga tcacattgtt attcgaagaa acagattata
    12241 attatcaaga agttcaatta ggagatgcga agcacatccg tgttttatat gaagatgagc
    12301 atttgattat agtggataag cctgcaggaa tgaagactca tccaaatgaa ccgacagaaa
    12361 acagtacctt actcaatcat ttagctgctt atctcgacac taaacagcaa cgaccttacg
    12421 ttgtccaccg cttggacaaa gaaacaagcg gtgccgtttt atttgcaaaa aatccatttg
    12481 tcttgccgat acttggaaga atgttggaac aaaaaatgat ctatcggaga tatcaagcca
    12541 tcgtatgggg agaaattgcc aaagatatga ccatcaccaa taaaattgga cgagatcgcc
    12601 atgatcggag aaaaagggtc gtagatgata aaaatggtca gagcgccttg acacatgtca
    12661 ctgtcaacaa agtactttct gaacaaacac aagtctattg tgttttggaa accggacgta
    12721 cacaccagat cagagttcat ttagctcata agggacatcc gatcgttgga gaccctctct
    12781 accaaaatag aacagctgaa cgactgatgc tccatgcatt agaactgcac atgatccatc
    12841 catttactca agaaaaaata gtcggtaaag ctttacctgg attatgggaa tagaaataaa
    12901 aaagaacaga gt
//
LOCUS       DAXWLB010000071        12839 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXWLB010000071 DAXWLB010000000
VERSION     DAXWLB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.071"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            133..3660
                     /locus_tag="ACGE4H_001571"
     CDS             133..3660
                     /locus_tag="ACGE4H_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFF8364988.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACGE4H_001572"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACGE4H_001572"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFF8364989.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACGE4H_001573"
     CDS             7448..7789
                     /locus_tag="ACGE4H_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFF8364990.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE4H_001574"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACGE4H_001574"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFF8364991.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE4H_001575"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACGE4H_001575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFF8364992.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE4H_001576"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACGE4H_001576"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFF8364993.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4347 a         2240 c         2724 g         3528 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggcaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gtgaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagg
//
LOCUS       DAXWLB010000072        12760 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXWLB010000072 DAXWLB010000000
VERSION     DAXWLB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.072"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..674)
                     /locus_tag="ACGE4H_001577"
     CDS             complement(<1..674)
                     /locus_tag="ACGE4H_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFF8364994.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKG"
     gene            1308..2279
                     /locus_tag="ACGE4H_001578"
     CDS             1308..2279
                     /locus_tag="ACGE4H_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFF8364995.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2178..2417)
                     /locus_tag="ACGE4H_001579"
     CDS             complement(2178..2417)
                     /locus_tag="ACGE4H_001579"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8364996.1"
                     /translation="MQNTKAQADLLPRLLLPQNVKQAYLELGGLPELDGRYTVFGQVY
                     QGLEIVAAIAAQPTNSEDVPIEPVFIRQINFEKTS"
     gene            complement(2392..2616)
                     /locus_tag="ACGE4H_001580"
     CDS             complement(2392..2616)
                     /locus_tag="ACGE4H_001580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8364997.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFAGEVHRGLY
                     HFNGALALGDHGPHTNGNQFFYCAKHKGAG"
     gene            complement(2585..2716)
                     /locus_tag="ACGE4H_001581"
     CDS             complement(2585..2716)
                     /locus_tag="ACGE4H_001581"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8364998.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2792..3175)
                     /locus_tag="ACGE4H_001582"
     CDS             complement(2792..3175)
                     /locus_tag="ACGE4H_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFF8364999.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(3208..3993)
                     /locus_tag="ACGE4H_001583"
     CDS             complement(3208..3993)
                     /locus_tag="ACGE4H_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFF8365000.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            4171..5271
                     /locus_tag="ACGE4H_001584"
     CDS             4171..5271
                     /locus_tag="ACGE4H_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8365001.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            5261..6085
                     /locus_tag="ACGE4H_001585"
     CDS             5261..6085
                     /locus_tag="ACGE4H_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFF8365002.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            6082..6897
                     /locus_tag="ACGE4H_001586"
     CDS             6082..6897
                     /locus_tag="ACGE4H_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365003.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6894..7724
                     /locus_tag="ACGE4H_001587"
     CDS             6894..7724
                     /locus_tag="ACGE4H_001587"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365004.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7731..8762
                     /locus_tag="ACGE4H_001588"
     CDS             7731..8762
                     /locus_tag="ACGE4H_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365005.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8869..10142)
                     /locus_tag="ACGE4H_001589"
                     /pseudo
     CDS             complement(8869..10142)
                     /locus_tag="ACGE4H_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10369..10554
                     /locus_tag="ACGE4H_001590"
     CDS             10369..10554
                     /locus_tag="ACGE4H_001590"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFF8365006.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10579..11298
                     /locus_tag="ACGE4H_001591"
                     /pseudo
     CDS             10579..11298
                     /locus_tag="ACGE4H_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(11296..11816)
                     /locus_tag="ACGE4H_001592"
                     /pseudo
     CDS             complement(11296..11816)
                     /locus_tag="ACGE4H_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            12104..12757
                     /locus_tag="ACGE4H_001593"
     CDS             12104..12757
                     /locus_tag="ACGE4H_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8365007.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3690 a         2364 c         2872 g         3834 t
ORIGIN      
        1 cctttgacgt agtagccgct ttccggatca gctttactta tttttctttc tttcagcttc
       61 acttcctttt ctaagtccaa agtaaaaggg cgttttccag cctctattcg ttgacgattg
      121 atctcatctt ctaattcatc ctgataggct ttcgcttcat catgggtcat ttctttcgta
      181 aacttatgtt tattggcgtt agctttgatg tgggtagaat ccagatacaa attatcctct
      241 gttacaaaac ctgctttaac tgcttgttct aaaatatagg caaaaatatc ctcaaacacc
      301 gtcgtttctc taaatcggcg aacataattc tttccaaaag ttgagaaatg ggggattttc
      361 tcctcgaagg agtagcctaa aaaccagcgg taagcgacat tggtatcgac ttccttaatg
      421 gtttgtcgca tggaacggat accgaataaa tactgaataa acaccaattt gatcagaatc
      481 actggatcga tacttgggcg acctaaatcg gagtaagtgg cttcgacgat tggataaata
      541 aattcaaaat caagcgcggc atcaatcttt cgaacgagat gatcttgagg aactagatca
      601 tcaatcgtaa gcatcgcaaa ttgatttcta ccattcgtat tgtttttaga catcatagct
      661 atcactcctt gcattcgttt tcataaatct attataaaag aaaagactgt agacatcatc
      721 cataaaatgg actttgtcta cagtctggat aacgtgattt tttgctgtaa tgacaaggtt
      781 ttagctgttt cagataccat gtatccaaaa atagtttatt gagaaaatag gagagtattt
      841 gatactaact agtaggaaaa aatgagataa aaagtaaatg gaatcattta aggtaatctg
      901 aaataaaaaa tatacagccc tgtggtactt tacctacaag gctgtatata ttttttactc
      961 tcagcgtttt tgactccgtg tctgactccg ttttattgat tttgaatgtt attcaatgac
     1021 catttaacag gtcaaatttg atgtttttga ttccgtatga cgccgtttga tatctagtga
     1081 tatagtgatt tgagtctaca tcgaataagg gaataaacca ataaaaaatg atttagaata
     1141 ttaaaataac aaaaaagagg actacctgta ttagtcctct tttttgacca gattttgacc
     1201 agtctatttt acatcctagt atatttatat gagctaattt tatgattaaa tggtaagttt
     1261 ttccttgtgt gttttagttt aaactagtgt gtaattcaaa aatctatatg gaaggaacag
     1321 tattaagaat agaacagggt tctctacatg atggtgctgg tttacggacc gttgtttatt
     1381 taaagggctg cccattacgc tgtgcctggt gttcgattcc cgaatcacaa agcaagcaga
     1441 ttgaaaaagg atttggccag accatgaccg ctgaagaggt aatggatgag attgagaagg
     1501 atgctgtttt ttactttcac tcggatggcg gagtgaccat tagcggaggc gaggcactgg
     1561 ttcaggcgga ttttgctaaa gaaattttgc aaaaatcgaa atacattgga atcaataccg
     1621 tactggaaac cagcttttgt ggtgcttata atgaaatcca aaaggtagct ccctatgtgg
     1681 atacattgtt tgtggatgta aaaatgttta ccagcaagct ccacaaacaa tggaccggct
     1741 tagataacca acaaattttg aagaatattc gtcgcttttt aattgagtat cctaactgcg
     1801 aagtgcgaat tcgagttcct gtggttccag gtatcaatat gaacctgacc gagctgctga
     1861 cgattgcttg ttttgtggca gatcttgatc ggttcgtacc gttagaatta ctaccgtacc
     1921 actgctatgg tatgcacggt taccaagcat tgggactgga gtatccgttg gcagacacgc
     1981 cagcgccttc acctaaagaa atgtttgcat tggcagatca actggctcgc acggttcctc
     2041 atttgctggt cacagcttta accaaaacat ttatttattt attgaatcca agctttatct
     2101 tatggataaa tgaaagacac tcactagctg cagccagctc aagaatagag cttttgctga
     2161 agtcagtgag tgtcttttta cgatgttttt tcgaagttaa tttgtcgaat gaacactggc
     2221 tcaatcggta catcttcact gtttgtcggc tgagctgcga tcgctgctac gatttctaac
     2281 ccttggtata cctgtccaaa aacagtataa cgaccatcca gctccggtag tcctccaagc
     2341 tccaagtatg cttgctttac attctgagga agcaagagcc tcggcagcag gtcagcctgc
     2401 gcctttgtgt tttgcacaat aaaaaaactg gttgccgttg gtgtgggggc cgtggtcccc
     2461 taaggccaag gcgccattaa aatgataaag tccgcgatgc acttcaccag cgaaaaagcc
     2521 ctcatagctg gaaacagctt catcaccaga accatccaaa gcgccacctt gaataacaaa
     2581 ttccttaatg acacgagccg aaaggtgtgt tgtcataaac gccttgctca gctagggagc
     2641 gccagttggc tacagctagg ggagcttggt caggaaataa gcggagacga atttcaccag
     2701 catcggtgac cagcacacaa acttcttcct gctgcggttt ttctaattga aagagaggaa
     2761 ggtttgcaac tgaaacgcct tgatcctgcc atcaaatatc gaaaatccgc cactcgacct
     2821 tcgcctgatc accaaggcga gccattccca ataaaacggt ataggcggtt tcatccgctt
     2881 gaaaggtcaa ttgcaactgt cctcgtaaat cgttgtagtc atctggggcg gctacttgat
     2941 aaaaaagatg aaactgcgca tgcaaatatt ccaaaggctg atctttaaag gctgcaaaat
     3001 aagcctgaac tttttcccgc tgcaagctgt cagccaccaa aaaattttcc agttgttgtt
     3061 ttggaacggc tgctaacaag gatcggcaaa ccgcctcaat tttcaacgca tcccaatgtc
     3121 gcgcaattaa ataatggatg ggtgcctctt gattgtagtc ccacaggttg ctcatttttt
     3181 tcaccacttt cttctattat ataggaatca caaaatcggc agcggcgtca ggcggcgttc
     3241 ttttccttga atgactagca acttttctac ctgacagtct gccaacagct gactcatttt
     3301 tttaaagccc agctgataat cttcggcaac atgagcatcc gagcctaagg taaagagctt
     3361 tccgccgaca ctttgataca aaggaacggc ataacggtaa aggtcttcgt tgccgtattt
     3421 caagaagctc ttggcattta actccatcgc caattctcgt tcaacaacct ttttgaaaat
     3481 cgttagcagc tgagcctcaa aatgctccgc gaattcttcc gctgtgaagg tgaagcggcg
     3541 taatccataa tcaaagtgag tcaaaatatc tccttctgga aaatccgtca agacctgcaa
     3601 catctgatca aaatattcct tggtagtttg aatgggatct ttggttaaaa ccacatcgtc
     3661 catataatca aagcggccat tttgatgaat acttaccagc tttaaatcat aaggatgctg
     3721 agcgaggtaa tccacgatcc gctcttcttg tcctggaaca acgccaatct cgatacctcg
     3781 cagaaatttg gtggaatagg tttcttttaa agagtccagt ttgtttatat aggctggata
     3841 atccggaatg tcatcttttc cggtagatgg attgtttaaa tcaaagtgat ccgtagaaac
     3901 gaaaaactcc ggctgatagg ctaaatagtt ttcaaatttt tcctctgaat caaaggaaaa
     3961 atacgtgtgg agatgctgat cataatactg catagggagc ctcctttttt ctactatcat
     4021 accgcaaaag ctttagaagg ttaacgggaa ttcgtcgaaa tttacaaacc atatacaacc
     4081 tttacattgt tcatgaaacc ttttcaagaa actgattctc tcattacaaa gctttgttag
     4141 aatggaatta tcgaaagggg agaagaatga atgaaaatga atgaaaaaat tcgtaacatg
     4201 actatcggtt tattttgtgc agcaacagta agtatcctgc tgacaggctg cggctcaggt
     4261 gaagcaacga agcaatcggc tgcggcagac gactctttag atacgattat agaaaaagcc
     4321 aaggaagaag gagagattgc cagcttggga atgccggata cttgggcaaa ttgggtaggc
     4381 acttggaacg accttgaaaa agaatacggc ttaagtcacg tggatacgga tatgtccagt
     4441 gcagaagaat tggctaagtt tgaatcagaa ggaaaaaatg gaaccgccga tattggtgat
     4501 gtcggtatca gcttcggtcc gttggctgaa agcaaagagt taaccttacc ttataagacc
     4561 aactattggg aggaaattcc ggattgggca aaggatgatg atggggattg gttgttaagc
     4621 tatacgggaa ccatttcctt tatcaccgat aaagagaatg tgaagcaagc accgactagc
     4681 tggaaggagc tgcaagaggg tgactatcaa gttagcgtgg gggatgtgac gattgccaac
     4741 caagcacaat ttgctgtatt ggctgcggcg attgccaacg gaggtgacga aaaggatatt
     4801 cagccaggtc tgaattacta caaaaaaatg gcagaaaaag gccgtttgtc caccgttgat
     4861 gccagcgtag caaatcttga aaaaggtgaa atcgatgtgg cagttgtttg ggacttcaat
     4921 ggactaaatt atcgtgacca aattgactcc gagcgttttg aggtaactgt gccaacagac
     4981 ggctccatca ttagcgggta tacaacctta atcaataaaa atgcccctca tcccaatgcg
     5041 gcaaagctag cacgggaata tattttatct gatgaagggc aaatcaattt ggcaaagggc
     5101 tatgcgcgac ctattcgcaa ggtagagctg cctaaagaag tagctgaaaa attactgccg
     5161 gaggatgctt atgaagcggc ggtgccagtt gaagacaatg aagcttggaa caagacctct
     5221 gtagagcttc cagagctttg gcaggcggag gtactgacct atgcaaagta gacttgacaa
     5281 actgattgta gttatactag acggctgtcg ctacgacaca gccgtcgccc agcttgggtt
     5341 catgaatcat ttagttgagc accagcaagc aagcttattg aaggtaaaaa gtgagctgcc
     5401 cagcaattca cgtccgttat acgaagtact gatgaccggc gttccagcct atgaaaatgg
     5461 catctatagt aattaccatg ctcagcgatc caaagagtgg tcgctgtttg aattagttaa
     5521 ggaagctggc ggaaaaacag gagccgccgc ttaccactgg tacagcgagt tgtacaatca
     5581 agccccctat aatcgtttgg ttgatcgcat ccagcatcag gaggagcgac tgattcagca
     5641 tggcatcttt tatagtgagg acacttaccc tgattcgcat ttatttgcgg atgcccatta
     5701 tttagtgaat cagtatcagc cagacttttt ggtcgtacat tcgatgaata ttgatgatgt
     5761 cggtcacaaa tttaccgccg attctcatga atatgcagca gcggttaatc aggcggacag
     5821 attattggct atttaccttc ctgaatggct gcaaaaaggt tatcaagtcg tggtgactgc
     5881 ggatcacggg atggatgcgc acggactgca tggcggcagc ttggctgcac atcgtgaagt
     5941 tcctttttat ttgatttcaa ataaacgcca aaagctgaag gaacaaactg taccacagct
     6001 gctggcggct ccattgtttt gctggttatt aggaattgca ccgtcagaga aaatgcagga
     6061 cattcaagaa ttgatgaggg gatgaaagag atgaaaaaac gcttggttct ggttcctgcc
     6121 tcctttatct tctgtgcatt tttactgctg ccgctgcttt ttatggttct gtgcagcttg
     6181 cacagtgatt taactaacca gtggacattg gccaattaca gtgatatttt cagcaacaaa
     6241 tattacacac aggcctttgt taacagcgta ctgattgctg tcagctccag tgtgattggg
     6301 ttaatcggga cgtttatttt atgtttatgc ttaatgaatc tatcgactaa attgcaggag
     6361 aaaatcacct ttgtttccaa tttggcagct aattttgctg ggattccgct ggcattctct
     6421 tttattattt tgttggggaa tgtaggtgtg ctgaaaatga ttttgccatt tctagcggac
     6481 ttcaatttgt attcttggtg gggattgacc atcacgtata cctatttcca aattccgtta
     6541 ggggtcttgt ttttgtatcc cagcatgaag gaagtgaaaa aggaatggct ggagtccgca
     6601 caattgctgg gcgcttcatc agcgtatgct tggttcaagg tggctttgcc ttttttgcga
     6661 ccatcgattg ccagcacctt tgtcatctta tttgctaatg gcatggggac ctatgaaaca
     6721 gcgtatgccc tgaccagcag taatgtaaat cttttaacga ttcgaattgc ggctttagtg
     6781 tcgggagatg tgttcgccaa gcctaattta ggcagtgcgt tggcagtcgc tttaggtgtc
     6841 ctgctggtaa cggcaatgtt gattattcaa aagaaaggca aggtggtgca gacgtgaaaa
     6901 agttaataaa accgattttg ttctgctttg tcctgtatta cttgctgccg attattggaa
     6961 cgattttgta tgccagctcc actaaatggt caaaatcgct tttacctagt gattttacct
     7021 tgcaatggtt ccaacagctg ctgacggaca gggaatttat tgctgcggtg ggtcgctcac
     7081 ttttattagc aggtgtggtc ttggtgacga ttctgttgtt gatgattccc accattattt
     7141 ggattcattt gtatttccct cgtttgaatc gttggctgga aaagctgctc ctgctgccgt
     7201 atgctttacc aggagtgatt ttggtaacgg ctttgcttcg aacgtatgct gaaactggta
     7261 tcccaatgtt tgtagtgttg gtaggtgcat tgtttattac ggcgttgccg attgtctatc
     7321 ttagtcttaa caaccagatg cgcttaatta atctgaagga attagtggat gcagcagaaa
     7381 ccttgggcgc cccgatgtct acgattatta tccaggtgct gtttccaaac atccgtatcg
     7441 gtgtgaccct agtttctctg atgatttttt cttcggtttt tggggagtac atgctgacga
     7501 acctgttgat tggcggtcga tttcagacgg tacgcatcta tatgctgcgg cggatgaacg
     7561 aaaacggcca tttggctagc gctgtgatgg ttttgtattt gctgtttatg gtgggaattg
     7621 ctttaactac ctttatgctg acttatcgac aaaagcaaca gctgtccctt aagcagacga
     7681 gggaagagaa gcagtcgaaa aagaagccct taaaggagtt gtgacggaaa atgtttttag
     7741 aaatacatga tctcaatctg ttttttgatg aaaaacagat tttagctatt gatgaaattc
     7801 agattgaaaa gggtgagctg gttactttac taggtcccag cgggtgcgga aagtctacgt
     7861 tgctgcgctg tatcacggga ttggaacaac caaagagcgg tgagattgtg ctggacaatg
     7921 agaatattgc cgataagcct acgaaggatc gcaatatcgg ctttgttttt caacagtatg
     7981 cattgtttcc aactatgact gtttttgaaa atgtagcctt tggtttgaag gtaaagaagc
     8041 tgagtcctga ggtgattcag gaaaaggttt ttgagatgct atcattggtg gatatgacgg
     8101 agcaggcaga taaaaatgtc cagtttcttt ctggcggtca acgacaacgg gtggcactgg
     8161 ctcgctcatt ggtcactgaa ccgaaagtcc tgctgctgga tgagccatta agtgctttgg
     8221 atgcccgaat tagaaaacag ctgcagcggg atttgcgcgc tatccagcaa tcgctaggga
     8281 tgacgatgat ttttgtgact catgaccaag aagaggcgat gcgaatcagt gatcggattt
     8341 ttgtgatgga agctgggcgg gtggctcagg tttccacctc gaaggaattg tatcagaatc
     8401 ctcagagccg atttgttgcg gaatttatcg gcaactataa tcgtttcgaa tggtatgagc
     8461 tgaatcagta tacccacgag ttttccgcta agggcaatcg ctgcatttat tatttaagac
     8521 cggagctgat tcaatttgaa ccggttcctc agggggttaa gattccggtt ctatggaagg
     8581 aatcatttat tttaggcaac attcaacggt atgtttttca aacgctggag ggcaaagaaa
     8641 ttttagtgga tcggttgaac gatcgggaag agaaaattag cgatgcctta tacattcagc
     8701 tgaaagatat tctagcaatc cctgtggaaa ttgatactca gcattttgca caacaaagct
     8761 agtgtatagt cggcaaacat ttctggctct ttgtcaaata ggactgatag agtgatataa
     8821 gaacatctaa ggtagaattg attttctgct ttagatgttt ttttatgttt aataagtgat
     8881 cagatgattg attaagaaaa ttctggttct catcctaacc cgtaagctat tcgtttcaat
     8941 gctttgatgt gggtatgcag attttctatt ttgccatttg agtaaggcaa ctctaaggct
     9001 aaacgaatgc cggattcata gtttataaac gcttttttta tatgatgcag ttctcttgga
     9061 accgacttgg gcattgtttt tacaagctga aagaagatgt ccgcttcttt gtcataaaaa
     9121 gcttgaagaa tttcttgata gtaagcataa gctattcgta gttcatcaga agtagaaagt
     9181 agacgttcta cgacttcact ttcggttaaa taggcccaac caaaattacg ccattttgtc
     9241 catttatcat aaacaagcgt gtcctgtcgt ttgatcagaa gtcgagataa agccttcact
     9301 tgtcgatagt gtttggcttg tattggatcg tttcggtcga agcttttcat gactcatagt
     9361 caaatatcct taaaactgcg aatgaggtgc ttgacgatgt gaaagcgatc cacaacgagt
     9421 ttggcatgag gaaagcactc ttttatcagc aaagcataag aggcgttcat atctgttacg
     9481 agtattttta cgtttaaacg tgcacgtcgt tcaaattgca taaaataaga aatgagatct
     9541 ttcttcttac gtgaaggaag aatatccagt aatttcccag tatcgccatc catacaagaa
     9601 aaagaatagg gaccgacttg atttttcaat gaacgaaatt catctaccaa tgaacagcga
     9661 ggcaaccaat ttattttcac gtttgtcatg gggagcagag aagcgagtgt tcgcgaaacg
     9721 gttgaccaag aaacgccgca atgtttagaa atcgtcgtca ttgcttgttt ttcagtcaat
     9781 tcctccaata ttttataatg tacttgtttg gcgataaaac agtttttttc aacaatgtag
     9841 gtattcgccg taaaataggt gtgacagcca cgacagaggt accgttgctt tgccaaagca
     9901 agataagtcc gttgattcgc acacttattg agaaggacca gcgatttttt ctttccgttc
     9961 ttaacgatca gcccatgttg attcacctgt ttacactgag gacagggctg aatcgtttta
    10021 atgagcgtgg cagaaacaac gatactgttg attgaatgta tttttttctt ctcgacatga
    10081 gtgatttgaa tgtttccatc tttgattcct agtaatagtt tgataagatg aagatcaggc
    10141 atacagaacg ctcctttgat tgtgttgtgg tgattcaatt ctaacggagc ttctgtatgt
    10201 tttgtttttt tatgctcaaa aggactgata gaatgattta ctatcagtcc tagaaattat
    10261 agcgccacat ttctttcacc tttgactttt cagacggcag tttttatgct gatactgtag
    10321 agagagcagg acacagcttt tgcttgtttt gggtaagggg gaaagaagat gaccaaagca
    10381 cttatttttg attttgatgg gacgatgatt gacagtgaat ggatgtttta cgagagtatt
    10441 catcactatt tactgaagcc tatttactgg agcagttcgg ctataaaatt ccttttgcag
    10501 actatcaaca aggtgttggg acggcagcag aactgtcctt ttatgaaggg ataagtcaag
    10561 cggttgggag accagtggat gctgaacatt ttgagcagcg gatcagcagc actggaaaat
    10621 cggagtagtc tcaattctga actgcgggag ctagactatt tttttgaagc acatccgatg
    10681 attaaggaaa aaattgattc ctttgtgacg atcgatgatc ttcaggtcgg caaaccagat
    10741 ccagaaggtt atttgttgtg cttgaaacgg ctgagggttg aacaggctgt tgtagttgag
    10801 gatagtccag tcggtgccca agcagcaccg catatgacag ttgatgtaca agcgttggat
    10861 gcagattttt tcgcctttag cgggcataaa atgtgcgggc cgacaggaat cggtgtatta
    10921 tatggcaaac gtcaattgtt agaacaaatg gagccagtcg aattcggcgg tgaaatgata
    10981 gattttgtca atctttacga tagttcatgg aaagagctgc catggaaatt tgaagctggt
    11041 actccgaaca ttgcaggagc aatcgattac ttgaatgaga ttggtatgga gaatattcat
    11101 cgttatgagc aagaactggt tgcctacgtt ttacctaaat tacaagcgat cggcggtatc
    11161 accatttacg gaccgcaaga tcctgaacat catactggcg tggttgcttt taatttggat
    11221 ggcatccatc cccgtgatgt ggcgactgct ctggatatgg aaggtgtcgc tgtacgttca
    11281 ggacaccatt gtgcacaacg gttcatcgac gatttctgtt tgactaattg gtcgtttaaa
    11341 cagtcgatga taatgccgtt tgagcggctc caatgtcgtg gaatctttga gtaagagttt
    11401 ccaatatcgc ttgagccgac ggtacttctt ctgattttca gattacgtga ttgattttcg
    11461 tacagttcta tgagaatcgc ctgcttcagt tcctttgtta gttgatgatg ttcatcaact
    11521 aagtccgttt tcgcagaaaa ggtctgccca caatcatagc atagaaaacg ggtacgtttt
    11581 aattctaaaa tagtcaactt ttctttgact ttcaacatct gagtacgtgt cgtataggaa
    11641 ccgttgcgga taatacgaat cgatccacag tgttcacaat gggaaggtga gtcatccaat
    11701 ctccctttaa taaactgcgc ttgaacgcct ttatattctt tttcaatcag ccattcttca
    11761 tcaaaaaata aatgtttatc tgttaatccg agtaattttc tagtatgatt atccatagag
    11821 cgactccttt ggtagggtga tgttttggtc gactaaattt taccaaaagt gagtctcttt
    11881 ttgagttgaa acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caacaacaca
    11941 aaatattata gaagcctttt ttctaaagga tttcaaggtg tctgtttgca tttattaaaa
    12001 aaaagaaaaa gtattggcaa cagaaggcgt caccaacact agatattata gagccataat
    12061 atgctgtata atatctagtg tataggctta agtggaggta tatatgaaaa tattaactgt
    12121 tgaagatgat aatatgataa gagagggaat aagtgaatat ctttcagaat ttggatatac
    12181 tgttattcaa gctaaagatg gaagagaagc cttgtcaaaa tttaatagcg atataaattt
    12241 ggttatctta gacattcaga taccttttat aaatggttta gaagtgttga aagaaattag
    12301 aaagaaaagc aatttaccaa ttctaatctt gactgcattt agtgatgaag aatataaaat
    12361 tgatgcattt actaatttag tagacggata tgtagaaaag ccattttcat tgccggtctt
    12421 aaaagctaga atagattctt taattaagaa gaattatgga catttagaga agtttgaata
    12481 taaggatcta tcggttaatt ttaatagcta tacagctaaa ataaatggtg aagaaataga
    12541 tgtaaatgca aaagaactag aagtattaaa atgtttattg gataataatg gacaagtgtt
    12601 aacaagaatg caaattattg attatgtatg gaaagatagc gaagaaactc cttatgatcg
    12661 agtaatagat gtatatataa aagaacttag aaaaaaatta caattagact gtataactac
    12721 tataagaaat gtaggatata aattggagag aaaatgaaaa
//
LOCUS       DAXWLB010000073        12564 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXWLB010000073 DAXWLB010000000
VERSION     DAXWLB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.073"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            7..540
                     /locus_tag="ACGE4H_001594"
     CDS             7..540
                     /locus_tag="ACGE4H_001594"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFF8365008.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1030..1554)
                     /locus_tag="ACGE4H_001595"
     CDS             complement(1030..1554)
                     /locus_tag="ACGE4H_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFF8365009.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1547..2020)
                     /gene="tsaE"
                     /locus_tag="ACGE4H_001596"
     CDS             complement(1547..2020)
                     /gene="tsaE"
                     /locus_tag="ACGE4H_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFF8365010.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2143..3123)
                     /gene="pta"
                     /locus_tag="ACGE4H_001597"
     CDS             complement(2143..3123)
                     /gene="pta"
                     /locus_tag="ACGE4H_001597"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFF8365011.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3276..3956)
                     /locus_tag="ACGE4H_001598"
     CDS             complement(3276..3956)
                     /locus_tag="ACGE4H_001598"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFF8365012.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4082..4951
                     /locus_tag="ACGE4H_001599"
     CDS             4082..4951
                     /locus_tag="ACGE4H_001599"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8365013.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4977..5492
                     /locus_tag="ACGE4H_001600"
     CDS             4977..5492
                     /locus_tag="ACGE4H_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFF8365014.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5539..6102)
                     /locus_tag="ACGE4H_001601"
     CDS             complement(5539..6102)
                     /locus_tag="ACGE4H_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8365015.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6157..6336)
                     /locus_tag="ACGE4H_001602"
     CDS             complement(6157..6336)
                     /locus_tag="ACGE4H_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365016.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6413..6922)
                     /locus_tag="ACGE4H_001603"
     CDS             complement(6413..6922)
                     /locus_tag="ACGE4H_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365017.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7018..7398)
                     /locus_tag="ACGE4H_001604"
     CDS             complement(7018..7398)
                     /locus_tag="ACGE4H_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8365018.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            7728..8477
                     /gene="pnuC"
                     /locus_tag="ACGE4H_001605"
     CDS             7728..8477
                     /gene="pnuC"
                     /locus_tag="ACGE4H_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFF8365019.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(8661..9938)
                     /locus_tag="ACGE4H_001606"
     CDS             complement(8661..9938)
                     /locus_tag="ACGE4H_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365020.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10017..10661)
                     /gene="pcp"
                     /locus_tag="ACGE4H_001607"
     CDS             complement(10017..10661)
                     /gene="pcp"
                     /locus_tag="ACGE4H_001607"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFF8365021.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(10860..11804)
                     /locus_tag="ACGE4H_001608"
     CDS             complement(10860..11804)
                     /locus_tag="ACGE4H_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFF8365022.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(11806..12492)
                     /locus_tag="ACGE4H_001609"
     CDS             complement(11806..12492)
                     /locus_tag="ACGE4H_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFF8365023.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3615 a         2642 c         2229 g         4078 t
ORIGIN      
        1 acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc ttatagtgct
       61 tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt ctatacattg
      121 atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat ccatcaagaa
      181 gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc atattttcaa
      241 aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt agcgggatgc
      301 ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt gaaaagtagc
      361 cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga aaatttgaac
      421 atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc ggaaatcctg
      481 ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac tataaagtaa
      541 aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc cgagtcacaa
      601 ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg tgaccgagga
      661 gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg catcaagtaa
      721 taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac ttattaccgc
      781 agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt ttgacctgag
      841 tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg agctaatacc
      901 gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca cgacttttgt
      961 cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct cactcttttc
     1021 ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt cacctagatc
     1081 tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc ggtaaaggtg
     1141 tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta cacccatttc
     1201 cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc cccaatattc
     1261 ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac tgtctgcctt
     1321 gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata aattattatt
     1381 gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca ggagttctgc
     1441 ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca aaagaatgtc
     1501 ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac tcatgatttt
     1561 cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg tatgagatta
     1621 gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt tccaaataat
     1681 cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc ccatcacctt
     1741 caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa atatccatat
     1801 gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc ggacttttga
     1861 tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt ttacccgcac
     1921 ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca attgcctctg
     1981 cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt ctcccgctct
     2041 tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa tgaactagtc
     2101 aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa tcaatgtttg
     2161 tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac gagacaaatc
     2221 agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt taccgaaacg
     2281 ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa agactgttgc
     2341 ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa cataagaagc
     2401 atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag cgattttagt
     2461 tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg agaagctcat
     2521 cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat ctacggcgat
     2581 ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa agacatattt
     2641 ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga tgccaggctt
     2701 tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga tcgctccaga
     2761 gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat tcacatcttt
     2821 taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa caaaagtatc
     2881 caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc tagatgtttt
     2941 gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta aaatgggttc
     3001 cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat cagtagcttc
     3061 cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat caaacaattc
     3121 cacaagatga cctccagacg tttttatatg tattatcata cactattcca gaaaaaaaga
     3181 acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt tcacatacaa
     3241 agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg cagttgccaa
     3301 tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa gggtttggaa
     3361 ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat gatgatatgg
     3421 cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa gataaacacg
     3481 attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct ttcccaacct
     3541 ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt gagtaaaagg
     3601 actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat tccaagatca
     3661 tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc aggttgcaca
     3721 gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc taaaatcacc
     3781 actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata catatctgga
     3841 tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag ataataatct
     3901 tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc tttcaaaaac
     3961 tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga caatcttcac
     4021 cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa cgataatcat
     4081 tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc atatgagtgt
     4141 ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg agttcatggt
     4201 cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag gaatcgattg
     4261 tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt cattgtttac
     4321 cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg ctaacggtat
     4381 ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga aacgaattga
     4441 aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa tggcgattgc
     4501 catggcctca gctaagctgg agttattgca catcacatac gtggacagta ttcgtgctat
     4561 tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag aagggccagc
     4621 tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg ttacttcctc
     4681 tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg ctgttgccca
     4741 tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg ataacttcaa
     4801 tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg cagaaatcga
     4861 agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg taggaaaagc
     4921 catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg atatagatgt
     4981 cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt gtcaaaaacg
     5041 aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc gatgcattgt
     5101 ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc ctgacattcg
     5161 gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag aaaatattca
     5221 attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa ggtgatatct
     5281 ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg gataaagcaa
     5341 cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat gctccagttt
     5401 gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa agtcgcgata
     5461 agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg cggaaatacg
     5521 catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat gaccactgat
     5581 catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag cctttggatt
     5641 tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca atttaggtaa
     5701 tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata cactgataga
     5761 gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg tagacgcatc
     5821 tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt cttcttcatc
     5881 tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga tacctcgtga
     5941 ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta aagtcttttg
     6001 ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta agatcaatgg
     6061 ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct ctccttttct
     6121 ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat cttcaatctt
     6181 ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc aataagggca
     6241 gacgactggc atttctttat cattatgttc gactacatta ttgacttctt cgtatgcaaa
     6301 tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc ctttcatctt
     6361 tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc ttctatgcag
     6421 atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa taaggacgtt
     6481 catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag tagctgactg
     6541 cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata tcaaaaagat
     6601 gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat ggattgactg
     6661 ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta ggattctcca
     6721 aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt tcgtttttta
     6781 cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata tcatccatgt
     6841 gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg agggcagcaa
     6901 caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa agaatatcaa
     6961 gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt gttatcttta
     7021 ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt tttttcctgt
     7081 aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt ctgcaattgt
     7141 ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt ctggatagtt
     7201 tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc ctagtttcag
     7261 atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt caaatcctaa
     7321 tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg ctatatgatg
     7381 gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga tctatattaa
     7441 tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc ctttttataa
     7501 gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg ttcccaatat
     7561 aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta tcataacaca
     7621 aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata gattgtgtta
     7681 gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg aagtcatatt
     7741 ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat cttttctttt
     7801 tcagtcttgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg ctgtccgtta
     7861 ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat gcggcaaaat
     7921 cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt gggttttcag
     7981 caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg gatatccccg
     8041 tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa ttcactggaa
     8101 aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca ggattcctga
     8161 ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt tctattagct
     8221 tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg tggttagctt
     8281 cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct gcaagtttgg
     8341 cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt accgtttcac
     8401 cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa tactacaacg
     8461 cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac ttttgtctca
     8521 cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat aagctgaaat
     8581 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     8641 tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc ctcatcattg
     8701 aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg agcttcagta
     8761 aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc agattggata
     8821 tctttcacta ctagttgcca tttttctttt gagactgtac ggtctttctt atcagcagat
     8881 tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac tatccgagga
     8941 atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca aataaataga
     9001 tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg agcagtgact
     9061 tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc aaaataagca
     9121 ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg taaaaatgcg
     9181 cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa gtcgtcaaat
     9241 agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact tattttcttg
     9301 atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac ttggcgaaat
     9361 tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat ccgacgattt
     9421 tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat attcttctgc
     9481 ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg atggatcgaa
     9541 gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc tagttttagg
     9601 atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct accacactca
     9661 caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt gaagtcagaa
     9721 aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct gattagatat
     9781 cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata gatctcccaa
     9841 cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct gatttctttt
     9901 ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt cctttctaat
     9961 tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt gctaaattag
    10021 tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc ttcaactgca
    10081 taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac tggacgatcg
    10141 atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc cgggaattgt
    10201 gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt tcctgctgta
    10261 taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc tttgattggc
    10321 aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa tggctgattt
    10381 ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc gaacgagact
    10441 gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg ctgttggatg
    10501 gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac tttgatgatt
    10561 tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg attcactttg
    10621 tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc ctccgtccga
    10681 ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg taaataaagg
    10741 aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa aaaaatagtg
    10801 ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc tctcttctat
    10861 tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc aataaacaac
    10921 tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg cacgatattg
    10981 aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa tgcaaccatt
    11041 cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat cccaaccgtc
    11101 atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac catgccaaga
    11161 gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc tactgcattg
    11221 gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc taaaagcatt
    11281 ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc taaaaatgta
    11341 cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa aacaccgacg
    11401 cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc aaagacaccg
    11461 atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt ttccatatat
    11521 tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc tggtatggtc
    11581 atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc tgcaataact
    11641 acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt tttatctgtt
    11701 aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag gatctccagt
    11761 aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt gcccctttcc
    11821 tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac ttgccacaac
    11881 taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg ttacgtcata
    11941 tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga tgaatagtac
    12001 ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta gttcttcttc
    12061 atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca tcggtaaaat
    12121 gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg caaagatcat
    12181 tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga atcgcttgat
    12241 caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg ccaacataat
    12301 gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa gttctaacat
    12361 tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca ttacgatcaa
    12421 tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta aaaggctaag
    12481 ataattttcc atatattttc ctccataaaa tgacaatatt tttttaatta taaagcaaaa
    12541 cacataaaaa atatagttgt tttc
//
LOCUS       DAXWLB010000074        12296 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXWLB010000074 DAXWLB010000000
VERSION     DAXWLB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.074"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            140..3715
                     /locus_tag="ACGE4H_001610"
     CDS             140..3715
                     /locus_tag="ACGE4H_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8365024.1"
                     /translation="MISNKKRWLKVALLVAFCQIAFLASMTTQAEEIVQETTVSQVSP
                     TSNEVEVQSTLPKEAVKEETPSEVVPETEAPSTNEKTVLDETIGKSQQPGDSPVVAEE
                     TPVEAHEAQAIPEKDKSLEESGSTEINEGTEPVQLEDSKEVTTPIESTKEEMDSNTSL
                     SSEKEEKTVLLTEPREPELKKVEEFVDTKKEAVSPTSSKEDTLIVQQTARSARVRRAT
                     NTAVLGGRKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYT
                     ATALENYLKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRI
                     DYDRRKAEIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNV
                     SIHDNEVWITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNL
                     TILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEI
                     KDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKD
                     LVDSTNPDNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETK
                     GKEGYKLFEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIW
                     DKYANNGKGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKY
                     VLNKTPYVFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFK
                     KDETGTTFVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDG
                     TVIALEVENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGV
                     LMDKEESITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFE
                     NVYRDGRLVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLND
                     LIVGQTYKVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSF
                     AEWLEAQSEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFK
                     EVKQASVVPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3815..4753
                     /locus_tag="ACGE4H_001611"
     CDS             3815..4753
                     /locus_tag="ACGE4H_001611"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8365025.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4772..5122
                     /locus_tag="ACGE4H_001612"
     CDS             4772..5122
                     /locus_tag="ACGE4H_001612"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365026.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDRRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            5122..5553
                     /locus_tag="ACGE4H_001613"
     CDS             5122..5553
                     /locus_tag="ACGE4H_001613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFF8365027.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            5821..7140
                     /locus_tag="ACGE4H_001614"
     CDS             5821..7140
                     /locus_tag="ACGE4H_001614"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8365028.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            7133..7297
                     /locus_tag="ACGE4H_001615"
     CDS             7133..7297
                     /locus_tag="ACGE4H_001615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365029.1"
                     /translation="MYEEIPATCGTRLEIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRLERMYPTS"
     gene            7836..9011
                     /locus_tag="ACGE4H_001616"
     CDS             7836..9011
                     /locus_tag="ACGE4H_001616"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8365030.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VPRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNANVWKPWGKLIQNVGKVNLTITPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
     gene            9516..9713
                     /locus_tag="ACGE4H_001617"
     CDS             9516..9713
                     /locus_tag="ACGE4H_001617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365031.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
     gene            9755..9883
                     /locus_tag="ACGE4H_001618"
     CDS             9755..9883
                     /locus_tag="ACGE4H_001618"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365032.1"
                     /translation="MIENFEYKLSRLPKKKGATQKELANKLGVNVQSVSNVEEDKS"
     gene            complement(10109..10657)
                     /locus_tag="ACGE4H_001619"
     CDS             complement(10109..10657)
                     /locus_tag="ACGE4H_001619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365033.1"
                     /translation="MAILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEYLFASHTKNFMCVEHFVN
                     TYLHLLKTQKICTFEAFQTDYLFILDREAYHAKQAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKGYQKDQ"
     gene            complement(10712..11005)
                     /locus_tag="ACGE4H_001620"
     CDS             complement(10712..11005)
                     /locus_tag="ACGE4H_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365034.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFQSV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKKTIEAVKIFFY"
     gene            complement(11205..11546)
                     /locus_tag="ACGE4H_001621"
     CDS             complement(11205..11546)
                     /locus_tag="ACGE4H_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365035.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVNFWQIIKLTEKTAWFRPIKK
                     QTVKTYTSMSGETMPIPNEFIDYPLVRTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            12047..12196
                     /locus_tag="ACGE4H_001622"
     CDS             12047..12196
                     /locus_tag="ACGE4H_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365036.1"
                     /translation="MKKKVIPYCPKPKNAFERGLFKYFEGKKGQDHKEENQKKKSKKK
                     SEEFF"
BASE COUNT         4322 a         1745 c         2464 g         3765 t
ORIGIN      
        1 tatagacaaa gatgttcata gcgaacatct tttttattta ttttgagaag aaacagagca
       61 tgaaatgatg tcttatttcc atccatatag tctatttcaa acagaagaga acaattaaaa
      121 agaaaggaga tttcttgtta tgataagtaa caaaaaacgt tggttaaaag ttgctttact
      181 ggtagctttt tgtcaaattg ctttcttggc ttcaatgaca acacaagcgg aagaaatagt
      241 tcaagaaaca actgtttctc aagtttctcc tacttcaaat gaagtagaag tgcaatctac
      301 tttaccaaaa gaggccgtta aagaagagac accatcagaa gttgttccag agaccgaagc
      361 accttcaacg aacgaaaaaa ctgtactcga tgaaactatc gggaaaagcc aacaaccagg
      421 agatagtcca gttgtggcag aagaaacccc agtagaagca catgaagctc aagcaattcc
      481 tgaaaaggac aaatcattag aagaaagtgg ttctactgaa ataaacgaag gaacagaacc
      541 ggttcaatta gaagattcaa aggaggtaac gactccgatt gaatcaacga aagaagaaat
      601 ggattctaat acttctcttt cttctgagaa agaagagaaa acagttcttt tgacagaacc
      661 tcgtgaacct gaattgaaaa aagtcgagga atttgttgat acaaaaaaag aagctgtttc
      721 tcctacctct tcaaaagaag atacattgat tgtacaacaa accgctcgaa gtgcacgggt
      781 gcgacgtgca accaatacag cagttttggg tggtcggaaa attgattggg gcgaaaatat
      841 tggtgttatt tctagtgcca ataatcccaa tttcacttta aattacatgg ttcgttttga
      901 gattaatgga caagtagcat tttgtattga acctggtgtc gcagccgcag aaggtcaagt
      961 ttatacagct actgctttag aaaactattt gaaagattta aatatacgaa aaaaaacaag
     1021 cttgatctct tatttaggat atgtcacaag caaagacaaa tccgatgaac aatacattgc
     1081 ggcacaattt atgatttggg aagttttggg cacaaaaatt ccaattaaaa atatccgtat
     1141 tgactatgat cgtcgaaaag cagaaattca acgtttgctt aataattttg atgtaagagc
     1201 tacttttcat aatcaaacac atacggtaaa aattggcgaa acgttaaaag taaaaagtgg
     1261 caatgacttt ctacaacgtg taaaagacat tcaaaagcca aaaggtgtta atgtatctat
     1321 tcatgataat gaagtatgga ttacaatcac aaaagagtca ccagaaaagg caaaaattca
     1381 tttcaatcaa attgaagcat taggcgtgcc attagcgtat cgtcgtccag gtgctcaaac
     1441 aattggggtg ctgaatcctt ttgtacctgg tgactctgtt gtcaatttga ccattttgaa
     1501 acaaggaaac attcgtgtct ttaaagaaga tgcagaaacc ggtgtgaaag cacaaggtgc
     1561 agcttcttta gaaaatgcag tgtatggctt gtatcaagca aatgggaaga aaatcaaaga
     1621 aattacattg aaaaatatca atggcaaagt acaggcagaa atcaaagatc ttgatcctgg
     1681 taaatatgtt ttaaaagaga tcaaagcacc aaaaggttat gtactttctg atgaagcaat
     1741 tgaaatcgtc gttgaacctg gaaaaactgt caatacgatc gcaaaagatc aagtgatcaa
     1801 aggaaccatt gatttagtca aagtcgcaaa taaagattta gtcgattcaa ccaatccaga
     1861 caacaaaccc aaattagctg gtattgaaat ctctttgacg tcaaaaacaa ctggaaaagt
     1921 ggtgaaaaca gttgtgacag ataatgatgg ttacgctact tttggtaaaa ataccgttgt
     1981 ctttgatacg tacattcttt ctgaaaccaa aggaaaagaa ggatataaac tatttgaacc
     2041 attcgaagtc acaatttctg aaaatggaca aaccttccat tatgttttag aagacaaagt
     2101 aatcgaacaa cgtcttaaag tggtgaaagt ggatcaagaa acagggaaag tcattgctct
     2161 agctggtacc caattcaaaa tttgggataa atacgcaaac aatggtaaag gtggctatgt
     2221 atcgatggca ttaccgaatg atacagaggt ttcagacata tttacaacaa acgagaaagg
     2281 gtactttgtc acaacggata cattgaaata cggaaaaaat cgttacgagt tgcgagaaat
     2341 caaagcacct gaaaaatatg ttctaaataa aacgccttat gtcttttcag taacagacga
     2401 caccaatacg attcaattaa tctacttcgc taaccgttta gcgaaagcca acgtagaggt
     2461 tttcaaatat gatactagta cgaattttga tcaaaaaacg gcgattgctg gtgttgaatt
     2521 tgacctattc aaaaaagatg aaacaggcac aacctttgtt ggtacattca aaacagacaa
     2581 aaaaggtaaa atcaatgtga aagacttatt ggttggcgat tacttcttta aagaagtgaa
     2641 accattagca gggtatctgg ccttagataa agacgtacca tttactgtaa cggttgataa
     2701 agacggtaca gtgattgcac tagaagtgga aaacaaacgg attccaccta aaattggtac
     2761 tacagctatc ggtaagtata caaaccaaca aaaagtgacc ccattgaaaa aagtaacgat
     2821 tacagacact gtaaaataca gtaatctaat catcggaaaa acttataccg tcaatggtgt
     2881 tttgatggat aaggaagaat ctatcacagc aggaaaagac gtaccgttgt tagtaaatgg
     2941 taaacaaatt accgcaagta aagtgtttgt cgcaaaaaat cctaacggaa tgatttccct
     3001 tgactttgtc tttgatgcta gtgcattagc tggaaaagag atagtcgtat ttgaaaatgt
     3061 ctatcgtgac ggacgcttag ttgcgtctca tacagatatt cacgataaag gacaaacggt
     3121 tcgtattgtg aaccctatga ttcatacaaa agcaacaggt caaaacggcg aaaaacaatt
     3181 acaagcttta ccaaatcaaa aagtgaaaga attggtacag ttgaacgact taattgttgg
     3241 tcaaacgtac aaagtgctcg tacaaggtta tattacacct gatggtgtga aattagacgg
     3301 tacaagtgct gaaaaaattt tcaaagcaga taagacaact ttagagttct tatttgaatt
     3361 tttggtagat ggacgtaagc tagcaggcaa aggattatct tttgcagaat ggttagaagc
     3421 ccaaagtgaa ccaaaagatg aaaacgatta tgaagttcga tttgaacaaa tcgcaaacca
     3481 caacgtagac ttaaaatcag aagatcaaac ggtttatttt gtagaagttc caaaagtacc
     3541 ggaaccaaaa attcctgaaa aatcacaacc ttttaaagag gtgaaacaag cttcagtagt
     3601 accaatgttg ccaaaaacag gatcagaaga tactgtatta tggatgatca ttggtgtgct
     3661 atttgtttta gcagcaggtc ttacatttta cgagttacgc aaagtcaaag actaagtagg
     3721 acaaacaagc ggttagatgc ctaatcgctt gtttggtttt aaaaaagtta ttgcataagt
     3781 aacctttgaa aagataagaa atgatctttg ttttatgaag aaaaaagaaa ggaaaaagag
     3841 aatgaagaag ctatttttcg catgtttatt tagttccgta gttcttgctg gtggaataaa
     3901 cgtttttgca gatgaaatag gtattacacc agttacgcca cctgtgatag aagaaacaac
     3961 acctgttgtt ccaattccag atatttctaa aatagatgag tctgttccgg atcaaccaac
     4021 tcctgttgat ccggatatat cttcagaagt aactgaacca acaacacctg ttgttcctga
     4081 tataacggaa aattcagaaa atgaaacacc agttgtatcg ccttctactg gagaaacaac
     4141 gaatgataaa gatactacac caactaccag tgagtcttat tcgggaacta gttcggtaga
     4201 taatgattca aaaatagacg attttagttc ttcatctact acaatagata caccaaaaga
     4261 aacagaaaag aaaaatgatt taggagctcc caaaaattta acacctgaaa atcatataaa
     4321 gactgtatcg gaaaaggaaa tgactgtttc agtcagttca gatggaaaaa ttgtacaagg
     4381 aacgggtacc gaaaaatcaa ttcctattgt gacaagtaat ttagaagaaa ttaatcatgt
     4441 cccaacatct gaaactcctt tacaagtagc gactggtcaa gcgattgttg gggtacaaga
     4501 tggtgtacca cttgtacaag ataatcaagg gaatttggta gaagatttat ccattcctgt
     4561 aaaaaaacta cctagcggca atattgaaat caaaacaatc gatgggaaaa caaaagtatt
     4621 gccaaaaaca ggtgaagaac ttcaaattag tttaactatc cttggttctc ttttaacagc
     4681 agtaaccggg ggcatttttt atggtaaaaa acaatggcgt catttgcgtc atatgtttaa
     4741 gaaaactatt tagtaaggag agagaataaa aatgggttta tcttatgttg caattagtca
     4801 ggaagaagta ttgggaaagt gttttaatca aaaacacgtt ggctcgacag gtgaacgaga
     4861 tcgtcgagga aatatgaaga aagtagagtg ggagattttt cagatgaact ctgaaaagat
     4921 gggagaaatt gaagtgcgtg tagctgtcgg tggtgcgcct aaagccatgc gatacaatgc
     4981 agaagtgtta ctggcaaatc ctagaatttc tcgtcgagca gatgtacgaa atattggaaa
     5041 tgacggtgaa acaaacaaaa tagcacgtaa taggttttat ttgttagtcg atcgtgtaga
     5101 agtaaaagga ggaaataact aatgacacta gaatttgatc aaggaattgt cccagggttg
     5161 gtaaaagaaa cactaggaga aatctatttt gtcaaggcac aacgtccacg tatgctgtat
     5221 gaagataatg aaaacacagg agaaattaaa gagcgtccag ttgaattttc aagtgctacc
     5281 caagaaacaa tgttcgtggt gaattttcca ccaaaaacaa atttggatgg attaagtttt
     5341 ggcgatgtgg tggaattaga agaggttgaa tttcgatttt ggatttctat tgatcgcgaa
     5401 tcaatgagta accgagcaga gaatgatctg aaagtatctg cgcttcgtta taaaaaaacg
     5461 ggaaaaaacg tttttcaaac gtcttcctct actcgtgcaa catatgtaaa agaaaaacaa
     5521 gactcaaata aagaaagtgc aaagaaagag taatggtttc cattgctctt tcttatttta
     5581 aagctagaag aagtagctga tgtggagaaa attgaattgg ctggctatat tattgggata
     5641 aatttttgaa tttttaagaa tttagatagt ggttagatac gattgattct ttgtgtattt
     5701 tgtattattt aagtattgtg tggtatttac attaagttaa tattgatgtt gttagtgtta
     5761 atgaaggttt tcttttatcc aatacattag tcactctatg atgcgaggag atgagaataa
     5821 gtgtttcaat ataaaggtat tcgtattcga aatcgacata aatatgcttc agaacgattt
     5881 attttttatc ttatgttgcc ggttctgttg ctaatgagtg gatttttttc tctcttttat
     5941 acaacgatca catgggaatg gtgctgtatt tattggataa tcgcatttgg tttagctggt
     6001 ctatttgcgt atatgtgttt gtatttttac ttttataatg tttatcagcg attggttcat
     6061 aaaggaaaat tagcaaagat gatacatagt cgtcgattga ttcttgaaag agtattgaaa
     6121 aatggacggc ggaaagtggt gtattatcca aaaatttttt atcactataa aaatggctta
     6181 attcatgttc gattgccatt agatttaggt ccctttcaag atcggtttaa tgagatggct
     6241 gggcaactag aagaagcctt tttttgtgat ttagtagaag agcaacgaga gaaaaactat
     6301 atttgttata cctttttata cgatgtggaa aaaaatcgta ttgggattcg agatataacc
     6361 gtaaaagatg gcataatcac cttaatgaag aatgttcaat gggcatttaa taaagcacca
     6421 catgccataa ttataggaag tactggcggt gggaaaacgt attttataat gtctttgatc
     6481 tatgcattat taccgtatgg tacgtttgat atttgtgatc caaaacgtgc agatttggct
     6541 agtttagaaa aaataccaat ttttaaagat caagtttttt atggtggtgg tattttatct
     6601 tgtttaataa atctagctaa agaaactgct aatcgatacg attttttaag agaacagccc
     6661 gagtatgaat tagggtacga ttatacttat tatcatctaa ctcctcattt tttggtgatt
     6721 gatgaatggg cagcatatta tgcatcgtta gatactaaag aaaaaaagaa ggcaatggga
     6781 tgtttgaacc aaattgcttt aatgggtcgt caatgtggcg tgtttttaat tttaggtacc
     6841 caacgtccag atacaaagta tttggacggc gcgatacgag ataattttgg tttacgtgta
     6901 acactgggaa gagtatccaa agctgggtat aagatgatct ttgaacatac agtaaaatca
     6961 ttatttaata agccgattaa aggtcgtggt tatattgatt cgggaaccga tcaagtacga
     7021 gaattttatg cgccatttat cgatataaca agatttaact tttttgcgga atttaaacaa
     7081 ctatctgatc aattaatggc tgaaaaaatc aaggagacga aagagggaaa aaatgtatga
     7141 agaaatccca gctacatgtg gaactcgatt agaaataaaa gatttaatga ttgactcttt
     7201 tttggcaatt gttttcagtg agaagagatt aagcttcttt atcaataaaa aagtagagaa
     7261 tcgttggaga ttagaacgta tgtatccaac gagttaactg attggttaca gttgccttat
     7321 gacaattatc gaaggcgaat atgacgggtt tttacttcaa ataaaagtgc tcgatcctat
     7381 agtgaccaaa ttgtcgaaaa agcagcggaa cagcctgttt tttatggacg attcaaagga
     7441 ctttcctgtt cttatgttaa aagtaaagac cggtctcgac tgtacctaca agtagaggat
     7501 attaacttgt cctttgcgtt gtcagaaaat taaatttgtc ctcattagaa tacggaagat
     7561 gctgtgcgtg tgctccacaa gcaaacaagc ctcagggcgg aaggacgcgg aagcgtccta
     7621 gcgttagcga aagccctgag gtgcagggtg cgttgtgggg cgtgcgcgta gcgcacgcct
     7681 aaaaccccca tattctaaca ggggggtaga atttatattc ggtggcaaag atgggtgatt
     7741 gaaacattga tataacacta ttttgcttca aaaattttgt gatatttttc ttcgctttgt
     7801 aacataaaat atcacaaaag aaaggaaaga tatatttgga tgggacacaa ttaaagcaaa
     7861 tgcggttaag tcggaatttg acgcagaaat tagtggcaga tacattagga tggccacaat
     7921 cttttttatc aatgatagaa aatgaccaac gaagttgctc gaaagaacaa gaagaagcac
     7981 tttgtgaatt ttatgcgaat ttttcaagtt ttacgagttt agaaattaaa tttgattatg
     8041 tccgtgtacg agttccgaca aatgatacga aaacagttgt ggaagaaatt ttgggaatgg
     8101 attttaactc ttttttttat gcagaaaccc gcttatatgg ttatattgaa atgtatcaaa
     8161 aaggcatgat ccgtgtatta aattctaaaa aaggtgacga taaaggagtg ttgatcgaat
     8221 tatctggtca aggctgtcgg aattatgaaa gtgtactgga tgaacgaaat gatagttggt
     8281 atcaattctt tacacgatgt gtctctttca atggtgtgcc tcgaagaatt gatattgcgg
     8341 tagatgattt tgaagaacta ttttctttac atgaatttgc acaaaaacta aatcgtggcg
     8401 aatttgattc aaaatttcga acctggcggt ttcaagaaga aaaacagttg ttagataatc
     8461 taagtagtgg cttatctgtt tatttaggta gttcacaaag tttgatgtat ttttgttttt
     8521 atcagaagaa ttatgaaatt gcaaaaaagc agaaacttcc gttggaagag gtcgaggtaa
     8581 aaaatcgtta tgaaattcgc ttacgtgatg caaaagcggt cagttttatt gaacaatact
     8641 taaataaagt agatttttca gatttaacga tggggatttt agccaatcag ttaataatgt
     8701 ttgagggaga gaaaaacgca aatgtgtgga aaccatgggg aaaattgatt cagaacgttg
     8761 gaaaagtcaa tttgacaata acaccagaaa agccaaatta taatcggaaa ttgatgtatt
     8821 taaagacaca acctgctcgt acaatgaaga ttgtaaaaac tgtggatgaa attttaggat
     8881 tgaatcgatt tggagaaatt ttagaagaaa cagaattacg ggaacaagat gaaaaaattg
     8941 tagaagatga ggtgcgtgct gtatatgaat atatacaaga aaatccagac gcgattcatt
     9001 atttgctata aagcttgcga taagatgggg gaaacatgat taaaacagaa gaccaatgat
     9061 ggggagagta tatgattgct tgttaacaat taataaaaga aacgatccta tgtgttgtaa
     9121 atgatctaca gttatttaaa gaatatttag attatcaaca atcaatcaaa atgttacatg
     9181 tagaaatagg ggaagtatta agctaccgta ttagtgattc gttaaaatat tcgttgacaa
     9241 agatttattt atatgcaaca caagtatgtg gatatacgaa aaagcagtaa aaacaatgat
     9301 gaaatatgac gatttgccaa gcaagatatc ctagatttac aacaagtatt ggaagaaaaa
     9361 ctgtatctat atatgaaaaa tagaggggaa aagaaatgat tcgattttta ttttctgtag
     9421 gttggcattt tttgaaatta ctgtttcttt tagtttgtat tgtctgtagt attatttggt
     9481 tattaacact ttaaagaaag gaatatgttg gaaaaatggg acagtatttt cttaccggtg
     9541 tttggtatgt attgaaatta gtgattattt taggtattgc ctatatttgt tttagaattt
     9601 tattatcggg agaaggaatg ccaacgccaa aagattttat cataagcgtg ttgaattttg
     9661 tggcggatca aatacaagtg ataattcatt gggcgcagca acgttttgga taaaaaaata
     9721 tatttaaaaa atttaatgtt tagaagtggg ctatgtgata gaaaatttcg agtataagtt
     9781 atctagattg ccaaagaaaa agggagctac acaaaaagag ctggctaata aattaggtgt
     9841 caatgtacaa agcgtttcta atgttgagga agataagagc taactacatt tagtaattta
     9901 gaaaaaaaca gtttctttct ttgatgcttc atcgattgaa ttatttggta cgaataggga
     9961 tcgaataatt gcagatgttc cattaatatt agaagatatg gataatttgt ttgaaaaaat
    10021 tcgaatgatc tttatgactg aaaaagtaat caaacaatta aaaaaaagtt tctgtttttg
    10081 atggaaaaaa actatcaaaa agaatcaatt attgatcttt ttgatagcct ttatttcgtt
    10141 gatggaatcc atttattagc tgtttcaaca atcgttgttg taaaaaagaa ttgttttgga
    10201 tgcgtaattc tggatagcct tcaatggctt gattttgctt ttcaaatgct tgttttgcat
    10261 gatatgcttc acgatctaaa ataaatagat aatctgtttg aaatgcttca aatgtacaga
    10321 ttttttgagt ttttaacaaa tgaagatagg tattgacaaa gtgttctaca cacataaagt
    10381 tttttgtgtg tgaggcaaaa agatattctt tagctaaaaa gttagtagta agtgtatcag
    10441 ataaataaaa gtaatctggt gcgcgatcat acatttcgac aaaatagtca taaagtaaag
    10501 caattttttg tttctctgct agaagatact ctatttcttt ggaataaaac tcatcaattt
    10561 gatgagtgtg tttaataact tgaacaaata atttcatata gtgttgaatg aattctgttc
    10621 gttcgattgg ttcaccaaag ggttgaagta tggccatcat tctttctcct ttcactcaag
    10681 ggaaaaatcc cttgagaatg aatacatgta tttagtaaaa aaatattttg actgcttcga
    10741 tggttttttt tccagtttgt tttgcgataa gtttaggtaa aatctgttgt aaccattgtt
    10801 ggtcagatac ctgatcgaag aagtagccga ttgttgttaa aatgagtgta tctgttgtat
    10861 gttcaactaa acgtacagat tgaaaaggac ttaacccagc aacatagttc aaaatatttt
    10921 catctgtttg ttcaaaagat cctaaatagt tattggtatt ccgatcgtaa gaatcaacga
    10981 taggaatagc atttgttctt agcattgcaa tctctccttg attagaaata ctcagttatc
    11041 attttttatt gatagattta gaagcaagaa ctcttaattt atgcgttgtt ggtaaattgt
    11101 cagaccgttc ttcacctaag atttcatata gagttattgg tttcattgtt cttgctcctt
    11161 ttatttttgt tatttttaac attgactaag agggaaattc cctcttagta ataactgctt
    11221 tcataaaccg gttgattatc atagcggtaa aatgtatccc aactgcgatt accgtataat
    11281 tcattgggat ataaggctgg attaattcgt tttctaacaa tgctgtcttt ggttcgtaca
    11341 agcggataat cgataaattc atttggtatt ggcatagttt ctccactcat agaagtatag
    11401 gtttttacag tttgcttttt gatgggtcga aaccatgctg ttttttcggt taatttaata
    11461 atttgccaaa aattaacgtt ggtttgttca tagccccaac tactataaaa aatgtctcct
    11521 actttgtatg gtgtatttgc tttcatgatc tattcctcca ataatgtttg atttgcctta
    11581 tttgatactt agccgaatca accaaccatt ttcggcagca agttgtctcc gaggtgggcg
    11641 accttttggg agatttcaat ttctttttgt ttctattcac ctcacttttt gtttttggtt
    11701 ggtcattttc gtatagctaa gcgttcgtcg gcgtgctttg ggttcacaac gtgtggtaag
    11761 agaaaacaag ggaatgctgt aggaaaaagt tttttatgaa atgatcaaaa aagtttttcc
    11821 gccccttgtt ttgtcttacc atccgttgta aaagcaccgt cgccgatgat cgcttagatt
    11881 gggtatatga aagtgaccaa caaaaaaagt atgggaataa aaagaaatcg cattgaaatt
    11941 tctcaaaagg tcaagatggt tttaataaaa actttctttt aatacatttg ttattttgat
    12001 aatgtggtga tataaaatag atcaaatagt taaatgaggt gtttatatga agaaaaaagt
    12061 tattccttat tgtccaaaac ctaaaaatgc ttttgaaaga ggtttgttta aatattttga
    12121 aggcaaaaaa ggacaagacc ataaagagga gaatcaaaaa aagaagtcta agaaaaaaag
    12181 tgaggagttt ttttagaaag aataaaactg gaacgtaaag atttacaagt aatcagtaac
    12241 ccgtaccttt tatttttaaa gaggtcgttt ttctgtaaat cgttgtaaag aatgac
//
LOCUS       DAXWLB010000075        12191 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXWLB010000075 DAXWLB010000000
VERSION     DAXWLB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.075"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(81..638)
                     /gene="frr"
                     /locus_tag="ACGE4H_001623"
     CDS             complement(81..638)
                     /gene="frr"
                     /locus_tag="ACGE4H_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFF8365037.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(641..1363)
                     /gene="pyrH"
                     /locus_tag="ACGE4H_001624"
     CDS             complement(641..1363)
                     /gene="pyrH"
                     /locus_tag="ACGE4H_001624"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFF8365038.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1499..2380)
                     /gene="tsf"
                     /locus_tag="ACGE4H_001625"
     CDS             complement(1499..2380)
                     /gene="tsf"
                     /locus_tag="ACGE4H_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFF8365039.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="ACGE4H_001626"
     CDS             complement(2479..3261)
                     /gene="rpsB"
                     /locus_tag="ACGE4H_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFF8365040.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3620..4099
                     /locus_tag="ACGE4H_001627"
     CDS             3620..4099
                     /locus_tag="ACGE4H_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8365041.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4316..5407
                     /gene="phnW"
                     /locus_tag="ACGE4H_001628"
                     /pseudo
     CDS             4316..5407
                     /gene="phnW"
                     /locus_tag="ACGE4H_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5400..6185
                     /gene="phnX"
                     /locus_tag="ACGE4H_001629"
     CDS             5400..6185
                     /gene="phnX"
                     /locus_tag="ACGE4H_001629"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFF8365042.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6531..8222)
                     /gene="argS"
                     /locus_tag="ACGE4H_001630"
     CDS             complement(6531..8222)
                     /gene="argS"
                     /locus_tag="ACGE4H_001630"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFF8365043.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8644..9591)
                     /gene="arcC"
                     /locus_tag="ACGE4H_001631"
     CDS             complement(8644..9591)
                     /gene="arcC"
                     /locus_tag="ACGE4H_001631"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFF8365044.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9706..10725)
                     /gene="argF"
                     /locus_tag="ACGE4H_001632"
     CDS             complement(9706..10725)
                     /gene="argF"
                     /locus_tag="ACGE4H_001632"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFF8365045.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10816..12045)
                     /gene="arcA"
                     /locus_tag="ACGE4H_001633"
     CDS             complement(10816..12045)
                     /gene="arcA"
                     /locus_tag="ACGE4H_001633"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFF8365046.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3346 a         2535 c         2191 g         4119 t
ORIGIN      
        1 ttttttaatt tttaaataaa agaaatataa ataaaaacag aggaagcgga atgatccgcc
       61 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
      121 ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      181 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      241 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      301 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      361 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      421 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      481 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      541 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      601 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      661 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      721 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      781 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      841 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      901 cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      961 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
     1021 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1081 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1141 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1201 cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1261 tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc
     1321 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1381 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1441 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1501 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1561 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1621 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1681 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1741 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1801 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1861 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1921 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1981 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     2041 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2101 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2161 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2221 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2281 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2341 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2401 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2461 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2521 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2581 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2641 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2701 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2761 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2821 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2881 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2941 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     3001 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3061 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3121 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3181 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3241 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3301 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3361 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3421 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3481 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3541 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3601 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3661 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3721 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3781 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3841 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3901 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3961 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     4021 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4081 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4141 tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt
     4201 attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4261 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4321 taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4381 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4441 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg
     4501 ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4561 ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4621 ctataccgtt tatgaggtat cgtaacatca aatcctccaa gctcatgtga ttacacagct
     4681 gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4741 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat
     4801 agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga
     4861 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4921 tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct
     4981 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     5041 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5101 aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat
     5161 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5221 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt
     5281 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5341 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5401 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5461 ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa
     5521 tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5581 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5641 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5701 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5761 cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt
     5821 attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt
     5881 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5941 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     6001 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6061 ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6181 aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa
     6241 cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6361 gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6421 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6481 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6541 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6601 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6661 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6721 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6781 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6841 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6901 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6961 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     7021 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7081 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7141 ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7201 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7261 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7321 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7381 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7441 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7501 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg
     7561 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7621 gctacttcat ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca
     7681 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7741 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7801 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7861 gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct
     7921 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7981 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     8041 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8101 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc
     8161 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8221 atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8281 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8341 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8401 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8461 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8521 tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta
     8581 aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac
     8641 aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc
     8701 taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc
     8761 tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc
     8821 tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga catacacatt
     8881 gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga
     8941 agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac
     9001 aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat
     9061 gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg
     9121 tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc
     9181 ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc
     9241 cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac
     9301 ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt
     9361 atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt
     9421 accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt
     9481 cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt
     9541 agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt
     9601 cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt
     9661 ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat
     9721 aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc
     9781 ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt
     9841 gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg
     9901 caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt
     9961 gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga
    10021 tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt
    10081 aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc
    10141 ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt
    10201 gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc
    10261 ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg
    10321 ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac
    10381 cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc
    10441 tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg
    10501 atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt
    10561 ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt
    10621 caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc
    10681 tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct
    10741 ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg
    10801 ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg
    10861 accaccacgg cctcgtgaca attcacttga attgatttct aacactttga tgccatagct
    10921 acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat
    10981 tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt
    11041 accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa
    11101 gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat
    11161 cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt
    11221 aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt
    11281 ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt
    11341 agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc
    11401 ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct
    11461 tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt
    11521 catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa
    11581 gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact
    11641 gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc
    11701 ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat
    11761 atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc
    11821 ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc
    11881 aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg
    11941 atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt
    12001 ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct
    12061 tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc
    12121 attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa acgtttttct
    12181 atttttaagc g
//
LOCUS       DAXWLB010000076        12136 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXWLB010000076 DAXWLB010000000
VERSION     DAXWLB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.076"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..610
                     /locus_tag="ACGE4H_001634"
     CDS             <1..610
                     /locus_tag="ACGE4H_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8365047.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACGE4H_001635"
     CDS             723..1001
                     /locus_tag="ACGE4H_001635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8365048.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4H_001636"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACGE4H_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFF8365049.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACGE4H_001637"
     CDS             1458..2213
                     /locus_tag="ACGE4H_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8365050.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4H_001638"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACGE4H_001638"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFF8365051.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4H_001639"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACGE4H_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFF8365052.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4H_001640"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACGE4H_001640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFF8365053.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4H_001641"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACGE4H_001641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFF8365054.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4H_001642"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACGE4H_001642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFF8365055.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACGE4H_001643"
     CDS             6950..7492
                     /locus_tag="ACGE4H_001643"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFF8365056.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4H_001644"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACGE4H_001644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFF8365057.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACGE4H_001645"
     CDS             9085..9987
                     /locus_tag="ACGE4H_001645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFF8365058.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4H_001646"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACGE4H_001646"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFF8365059.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACGE4H_001647"
     CDS             11430..11852
                     /locus_tag="ACGE4H_001647"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFF8365060.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAXWLB010000077        11976 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXWLB010000077 DAXWLB010000000
VERSION     DAXWLB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.077"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(157..321)
                     /locus_tag="ACGE4H_001648"
     CDS             complement(157..321)
                     /locus_tag="ACGE4H_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365061.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            616..1944
                     /locus_tag="ACGE4H_001649"
     CDS             616..1944
                     /locus_tag="ACGE4H_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFF8365062.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2146..2979
                     /locus_tag="ACGE4H_001650"
     CDS             2146..2979
                     /locus_tag="ACGE4H_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFF8365063.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2995..3732
                     /locus_tag="ACGE4H_001651"
     CDS             2995..3732
                     /locus_tag="ACGE4H_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFF8365064.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3887..4855)
                     /locus_tag="ACGE4H_001652"
     CDS             complement(3887..4855)
                     /locus_tag="ACGE4H_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFF8365065.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5087..5920
                     /locus_tag="ACGE4H_001653"
     CDS             5087..5920
                     /locus_tag="ACGE4H_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFF8365066.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5954..6793
                     /locus_tag="ACGE4H_001654"
     CDS             5954..6793
                     /locus_tag="ACGE4H_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8365067.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6819..7379
                     /locus_tag="ACGE4H_001655"
     CDS             6819..7379
                     /locus_tag="ACGE4H_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8365068.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7578..9299
                     /locus_tag="ACGE4H_001656"
     CDS             7578..9299
                     /locus_tag="ACGE4H_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFF8365069.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9464..10606
                     /locus_tag="ACGE4H_001657"
     CDS             9464..10606
                     /locus_tag="ACGE4H_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFF8365070.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10622..11833
                     /gene="thiI"
                     /locus_tag="ACGE4H_001658"
     CDS             10622..11833
                     /gene="thiI"
                     /locus_tag="ACGE4H_001658"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFF8365071.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4074 a         2023 c         2490 g         3389 t
ORIGIN      
        1 tcgagtccta ctgccggagt aaataaagct tatgcaaaga aaaagaaatc caatcacttg
       61 ggtttctttt tcttttatta ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg
      121 taatctttac gaaagagtag agttggctac tcataatcaa ccatacttcc catcatcgct
      181 aaatacgaaa taaatcagca ttcctacacc aatcaaagca caagctgcac caagaaaagg
      241 gatgacattc ccaaagttat ctgtattcat aagtatgcca ccacccacaa acaaaaagcc
      301 gccagcaatc aggtttttca ttttattcat ctcctttctt acgttatagt atttccttct
      361 ttcaatgagt attttgaaag ctatcttatt gtatgatgtt cttctaggcc tcttctttat
      421 tatacaggcc attttcgtta tctcatagaa atattccttt ttatctgaaa actattgtat
      481 accgtatgac atttttcttc atgaagttgt aagtatattc tcattcaaat gaaaatttca
      541 tcatagaaga gattgtattt ctaaattctt aagagtaaaa tacgaagtat aacttggatt
      601 tggaaggatg attggatgaa agctattacg cctgatatga caacaaaatt taacaaacag
      661 tttcaagcaa atatcaaaca gcaggcattg cagcgggcag ttgtgaagaa cggaattact
      721 gcttctgctg aaaatgtttc agcacatgtg gagaatacac cggtattttc tatagatttg
      781 gctacgggga aagtggctaa tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg
      841 aacacattcc gtcataagat gctgaatcaa ttccatttaa aagagtttga attatcacaa
      901 aattacactt ttttctggga taaatatgaa aaggcaaatt atttctatga aaacatcatt
      961 gcaacaggaa aagaggcaat cgatagccgg aaagtagctt tcttactcgc tactcctcaa
     1021 caagatggcg gccaatggga tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct
     1081 aaatcggtga tgccggaaag tgcgaatagt tcaaattctc gagatttgaa taactacttg
     1141 aacaaattac tgcgtaaaga tgcagtagtg ttacggaaaa tggttgctca aggagaaaca
     1201 ttagacgcta ttgaagaaaa gaaagaagaa atgctagaaa gcatttataa cttcttagct
     1261 atctcattag gaacacctcc aaaagaattc gattttgagt accgcgatga agaaaagaac
     1321 tatcatcttg accgaggact gacaccacaa attttttatg ataaatacat cggtgtaaaa
     1381 ttagatgatt atgttagcgt gatcaatgca ccaacaaaag acaaaccatt caatcgttct
     1441 tatacagtag agatgctagg aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt
     1501 gatatggaaa ctttcaaaaa attagcgatt gctcagcttg aagaaggaga atctgtttgg
     1561 tttggttgtg atgtaggtca atcttctact agaactagtg gcatcatggc cttagatgct
     1621 tattcaatgg atgatctatt tgatacggat tttacaatga ctaaagctga acgtttagat
     1681 tttggagaaa gtctgatgac ccatgcgatg gtattgaccg gtgttgactt agtagacggg
     1741 caatcaacaa aatggaaagt cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc
     1801 tttgttatga gtgatgcctg gatggacgaa tatacgtacc aaatcgttgt aagaaaagat
     1861 ttattgacga aagagcaatt aaatgcgttc aatgaagaac caattgttct atctccgtgg
     1921 gatccaatgg gtgctttagc atagacgagt atttcaatga agagtatgta gtaaagataa
     1981 aaagagctgg cttaagccag ctctttttat ctttaaagtc cattccctgt tttttatggt
     2041 ccctgtgcta taattctaaa taaaggataa ggattggaaa taatcctttt atcattaaaa
     2101 atatgatgac gaagtagaat aaaattggga gtagggggga aagtcatgga tatccaaact
     2161 gttttatctg atctgaaatt aaatggtaaa gtgattccag ttgttggtgg cgatgttaat
     2221 cagacctatc gtataaaaac cgaacatcgt gcttattttt taaaaatcca tccgaatgta
     2281 aaaaaaggat tttttgaagc agaagtagac ggattaaaag aactgtcagc attcgtacgt
     2341 gtaccggata cgtatatgct aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg
     2401 atcgagccgg gaaaagggga tcaacgagat cttgctgcag ctttagctaa tctccatcag
     2461 caaacagcgc cacaatttgg ttttcgtaaa gataactatt tgggtacact tgttcagaaa
     2521 aattcttttg aagaagattg gtggactttt ttctttaaag atcggcttga atcccagatt
     2581 tctttggcag aagaaacgaa tcgatggaat gtacagcgtc aagaaaagta tctgcgattt
     2641 aaagaacgtg tattgaaatc ggttgaacca aaaaagatca ctccacgcct cttgcacgga
     2701 gacctgtgga gtggtaatgt gttctttgac cagcaagggc atccagtttt tgtcgaccca
     2761 gctgtttctt atgggaatcg ggaacaagat attgcaatga gccagttatt cggtggtttc
     2821 cgaccggaat ttttagatgc ttatcaaact atatttccat tagaaaaggg atggaaagac
     2881 cgactgccga tttaccagtt atattatctt ttggcacatt tgaatatgtt tggagaaagt
     2941 tacggttccc aagtagatca attactagaa aatttttaag aggagggcgt gtttttggca
     3001 aaaaaatata ccaaaaagca tgaagtttca tattatgagt gtgacatcaa ccaaacgatg
     3061 acatttcctt cgatgttagg agtagttatc aaaacgtctg aagatcaaag cgaccagttg
     3121 ggaagaggca gtgaattcgt caatagcttt ggacttactt gggtcatcac gaattattcc
     3181 atggagatta atcgtctccc tagagtcggt gaagtaatct cagtaacgac tcaagccatg
     3241 gagtacaata agtttttctg ttatcggaac ttctggatcc atgatgaaga tgggaaagaa
     3301 ttagtcaaaa tagaatctgt tttcgtattg atggattttg tgaatcgaaa aatgagcagt
     3361 gttaatgaag agatcattgc tccttttgag agtgagaaga tcaagaaaat caagcgtcag
     3421 gaaaaaattg aaaagattga ttcgggcttc atgttgccat atcgtgttcg tttttatgat
     3481 atcgatagta atcagcatgt taataatgcg atgtatttta attggatcat tgacgtatta
     3541 ggatatgact ttttgactac gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa
     3601 gtagagtacg ggaatgagat cgaaagccat tttgaacaaa tccctatcga aaatggtgtc
     3661 aaaacaaaac atgaaatccg aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg
     3721 aaaaataaat aaaaaagagg ttgtgacaga agggggcaac ttcaagaaat aagaagaaat
     3781 ttacgaaaat tgtttctcaa atttttgtaa attttcgact tagttttcga agaagctatt
     3841 tttgtcacag cctctctttt taagtttagc tattagataa tgatgtttag ttttggataa
     3901 acgaaatcaa ttcttcaccc gacaaaccat agtttacagc aatcaatagt tttaaacgtg
     3961 ctttttgact attaatactg ttacagaaaa taatccccat tttttctagt tcttttcccc
     4021 cgccttcata atcataaaca ggctctgcga ttccattgaa acaacgagaa acaagtacta
     4081 cagggatttt ttggacaaga agcttttcca aagcagctaa ggcttgtgga gggagattcc
     4141 ccgcacctaa agcttcgatt accaaaccgt ctactttcgt atgggcgatg gcttccaata
     4201 agtccccttg catcccagca tatgctttaa cgatcgggat agtcccatct acagcggaaa
     4261 tgtccagtcg ctttgtttct aaaagttcct gcaagaataa aattcgattc ttagttacta
     4321 agccgatggg acccaatgta ggtgttcgga aagtagcaac atttgtggta tgggtctttg
     4381 tcacgtagcg tgcagaatgg atctcatcgt tcatgaccac caaaaccccc ttatctagcg
     4441 cttcttcaca acttgcgaca cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa
     4501 gttcattact tgaacgcatt gccccagtca aaacaattgg taatttgttg ccaatcgttg
     4561 tatcaagaaa aaaagcagtt tcttctaatg tatctgtacc gtgtgtgatc accactccgg
     4621 atatacctga tgcataagcg gacaagatac gttcttttaa taccaacatg tgagaaggca
     4681 tgatgtgtgg tgagggaaga ttgaagatat cttcaactaa taattctaca tgcttgggga
     4741 ttgtgatttc tgcatctaat aatgggtttg ccacatcagg actaacaccg cccgtgagca
     4801 tgttttcttt catggcgatc gttccgccgg tgtgtagtac aagtattttt ttcattttac
     4861 agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact atataaaaga
     4921 gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag ctgaccttat
     4981 taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg ttgagtttct
     5041 tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg
     5101 attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca ctctttatga
     5161 atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa ttccccgaaa
     5221 gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg tagtcaaagt
     5281 ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg aagaagagat
     5341 gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga tgttttgctt
     5401 tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa
     5461 acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt attcttggta
     5521 cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt tttctttagc
     5581 catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat ttgtcaccaa
     5641 tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc aagcaaatga
     5701 taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac tgcaagaact
     5761 agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga tttttaataa
     5821 taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat taaatatcaa
     5881 gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt tattcaagaa
     5941 aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat tggtcaccaa
     6001 tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga aaaacggcat
     6061 tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt
     6121 ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag atcgcacgat
     6181 tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg cagatgagaa
     6241 ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga cgatgctatt
     6301 aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat tcccggtacg
     6361 tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc gttatgaaac
     6421 gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg agtcagaaca
     6481 gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata cgtatactgt
     6541 agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg caaatgctgt
     6601 cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata tcgagatgct
     6661 gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa aacatgctgc
     6721 agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga aacacttcgg
     6781 tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag acaccttttc
     6841 aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata cccacggctt
     6901 ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt gttgaatttg
     6961 ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag
     7021 cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt gatccgctgg
     7081 ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct ttttcattac
     7141 taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg gggaaattat
     7201 ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa cctgaaaact
     7261 ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt cagattcaag
     7321 aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa aaagagtgat
     7381 cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt ttcgtttaag
     7441 ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt gcacgagaga
     7501 cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga tgcatactga
     7561 aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta
     7621 tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac caagatcgat
     7681 tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt gaagaagtcg
     7741 atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt gaatggagtc
     7801 aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa attttcgaag
     7861 tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt gttgaagcag
     7921 aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat
     7981 tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac gtctatgaag
     8041 aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt tttgaattgc
     8101 aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta aacacttctg
     8161 gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg tatgaagtgg
     8221 aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact ttccctgaac
     8281 aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat gtattcccag
     8341 aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat tcattagcca
     8401 atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga
     8461 ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat gtaaaaacta
     8521 accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt caattattga
     8581 ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt ggccgtgtac
     8641 gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg attccacaga
     8701 tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag
     8761 tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat gaattgagaa
     8821 aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg cgcagtttga
     8881 aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta gaattctttt
     8941 ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca
     9001 tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta ttagataaga
     9061 agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa tatgcaaatc
     9121 gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac tatttattca
     9181 ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta gagcgagtag
     9241 aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac ttagtataaa
     9301 gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc ttgtcttttt
     9361 ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag caccttggac
     9421 aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat attttgataa
     9481 cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg
     9541 gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc ggttgttgca
     9601 gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa tctattttac
     9661 ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg aaaaaagaga
     9721 atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg agacagcaga
     9781 acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac gcggatttgt
     9841 acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct ctgttatggc
     9901 tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc ttttggatga
     9961 ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag taccattctc
    10021 gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat tccatggccc
    10081 acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat tactgacagg
    10141 tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta ttgtggctat
    10201 ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc agacacaacg
    10261 cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga ttttttcaga
    10321 aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc gcggcgaagt
    10381 tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg cctgctcctc
    10441 acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg atctggctac
    10501 atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg agcaatttat
    10561 gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga ggagtgttct
    10621 tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag gaaagaatcg
    10681 aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag actttccaga
    10741 aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg cagacgggca
    10801 gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc caagtattcg
    10861 tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga aagaaattta
    10921 tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat ttgaattgac
    10981 aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc cgcatatcca
    11041 agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg atggcgctta
    11101 tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa gcggaaaagg
    11161 aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg ccatgaagcg
    11221 gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg aacaagcact
    11281 gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga tccattttat
    11341 cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac aaggatattg
    11401 gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca
    11461 tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag
    11521 tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag cgacaatgga
    11581 taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt ctatccagcc
    11641 ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc ctcaactgga
    11701 aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac gagcactagc
    11761 tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg aagaattttc
    11821 agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga gaaacaaaac
    11881 gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt agctgattga
    11941 ataccattta tcaagaagtt atggaaaagc cggttc
//
LOCUS       DAXWLB010000078        11937 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXWLB010000078 DAXWLB010000000
VERSION     DAXWLB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.078"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..241)
                     /locus_tag="ACGE4H_001659"
     CDS             complement(<1..241)
                     /locus_tag="ACGE4H_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8365072.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTI"
     gene            complement(247..1110)
                     /locus_tag="ACGE4H_001660"
     CDS             complement(247..1110)
                     /locus_tag="ACGE4H_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365073.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(1124..2020)
                     /locus_tag="ACGE4H_001661"
     CDS             complement(1124..2020)
                     /locus_tag="ACGE4H_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365074.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(2195..3484)
                     /locus_tag="ACGE4H_001662"
     CDS             complement(2195..3484)
                     /locus_tag="ACGE4H_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8365075.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(3456..4973)
                     /locus_tag="ACGE4H_001663"
     CDS             complement(3456..4973)
                     /locus_tag="ACGE4H_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFF8365076.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            5171..6061
                     /locus_tag="ACGE4H_001664"
     CDS             5171..6061
                     /locus_tag="ACGE4H_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFF8365077.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(6134..6958)
                     /gene="nadE"
                     /locus_tag="ACGE4H_001665"
     CDS             complement(6134..6958)
                     /gene="nadE"
                     /locus_tag="ACGE4H_001665"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFF8365078.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(6970..8442)
                     /locus_tag="ACGE4H_001666"
     CDS             complement(6970..8442)
                     /locus_tag="ACGE4H_001666"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFF8365079.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(9079..9441)
                     /locus_tag="ACGE4H_001667"
     CDS             complement(9079..9441)
                     /locus_tag="ACGE4H_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFF8365080.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            9664..10416
                     /locus_tag="ACGE4H_001668"
     CDS             9664..10416
                     /locus_tag="ACGE4H_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8365081.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(10556..11479)
                     /locus_tag="ACGE4H_001669"
     CDS             complement(10556..11479)
                     /locus_tag="ACGE4H_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8365082.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(11494..11766)
                     /locus_tag="ACGE4H_001670"
     CDS             complement(11494..11766)
                     /locus_tag="ACGE4H_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365083.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         3738 a         2414 c         1956 g         3829 t
ORIGIN      
        1 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
       61 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
      121 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
      181 ataagatgta atcagaaacc acaatcaact gtccataaag tttagtctca actattttca
      241 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
      301 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
      361 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
      421 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
      481 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
      541 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
      601 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
      661 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
      721 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
      781 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
      841 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
      901 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
      961 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
     1021 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
     1081 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
     1141 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
     1201 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
     1261 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
     1321 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
     1381 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
     1441 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
     1501 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
     1561 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
     1621 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
     1681 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
     1741 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
     1801 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
     1861 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
     1921 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
     1981 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
     2041 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
     2101 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
     2161 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
     2221 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
     2281 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
     2341 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
     2401 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
     2461 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
     2521 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
     2581 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
     2641 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
     2701 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
     2761 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
     2821 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
     2881 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
     2941 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
     3001 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
     3061 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
     3121 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
     3181 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
     3241 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
     3301 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
     3361 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
     3421 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
     3481 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
     3541 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
     3601 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
     3661 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
     3721 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
     3781 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
     3841 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
     3901 gtcctgattg gtgcaataac atttaccaat tgagcaagac aagcaatctt tactctatct
     3961 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
     4021 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
     4081 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
     4141 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
     4201 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
     4261 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
     4321 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
     4381 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
     4441 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
     4501 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
     4561 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
     4621 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
     4681 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
     4741 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
     4801 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
     4861 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
     4921 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
     4981 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
     5041 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
     5101 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
     5161 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
     5221 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
     5281 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
     5341 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
     5401 cgataaaatc gatatcagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
     5461 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
     5521 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
     5581 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
     5641 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
     5701 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
     5761 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
     5821 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
     5881 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
     5941 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
     6001 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
     6061 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
     6121 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
     6181 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
     6241 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
     6301 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
     6361 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
     6421 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
     6481 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
     6541 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
     6601 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
     6661 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
     6721 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
     6781 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
     6841 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
     6901 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
     6961 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
     7021 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
     7081 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
     7141 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
     7201 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
     7261 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
     7321 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
     7381 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
     7441 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
     7501 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
     7561 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
     7621 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
     7681 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
     7741 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
     7801 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
     7861 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
     7921 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
     7981 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
     8041 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
     8101 taatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
     8161 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
     8221 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
     8281 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
     8341 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
     8401 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
     8461 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
     8521 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
     8581 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
     8641 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
     8701 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
     8761 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
     8821 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
     8881 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
     8941 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
     9001 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
     9061 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
     9121 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
     9181 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
     9241 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
     9301 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
     9361 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
     9421 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
     9481 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
     9541 acaaaaggat ttacctttta tcacttaata gtataacata atttcgtgag aaaaaaatac
     9601 aattttttct tgaccatcta gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
     9661 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
     9721 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
     9781 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
     9841 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
     9901 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
     9961 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
    10021 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
    10081 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
    10141 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
    10201 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
    10261 tggttctctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
    10321 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
    10381 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
    10441 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
    10501 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
    10561 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
    10621 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
    10681 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
    10741 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
    10801 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
    10861 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
    10921 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
    10981 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
    11041 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
    11101 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
    11161 accttcgctc tttttcttta tgatagcccg tcggtaaaag tgtcactttg taatcataat
    11221 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
    11281 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
    11341 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
    11401 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
    11461 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
    11521 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
    11581 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
    11641 ttcttttact atatttctat tcaacccttg aaaactgtac aacgttaata cggatccctt
    11701 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
    11761 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
    11821 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
    11881 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaat
//
LOCUS       DAXWLB010000079        11933 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXWLB010000079 DAXWLB010000000
VERSION     DAXWLB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.079"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..226
                     /gene="yaaA"
                     /locus_tag="ACGE4H_001671"
     CDS             <1..226
                     /gene="yaaA"
                     /locus_tag="ACGE4H_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFF8365084.1"
                     /translation="LNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGEPENRRG
                     RKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            213..1337
                     /gene="recF"
                     /locus_tag="ACGE4H_001672"
     CDS             213..1337
                     /gene="recF"
                     /locus_tag="ACGE4H_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFF8365085.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1334..3280
                     /gene="gyrB"
                     /locus_tag="ACGE4H_001673"
     CDS             1334..3280
                     /gene="gyrB"
                     /locus_tag="ACGE4H_001673"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFF8365086.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3300..5771
                     /gene="gyrA"
                     /locus_tag="ACGE4H_001674"
     CDS             3300..5771
                     /gene="gyrA"
                     /locus_tag="ACGE4H_001674"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFF8365087.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6007..6306
                     /gene="rpsF"
                     /locus_tag="ACGE4H_001675"
     CDS             6007..6306
                     /gene="rpsF"
                     /locus_tag="ACGE4H_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFF8365088.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6356..6868
                     /gene="ssb"
                     /locus_tag="ACGE4H_001676"
     CDS             6356..6868
                     /gene="ssb"
                     /locus_tag="ACGE4H_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8365089.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6894..7130
                     /gene="rpsR"
                     /locus_tag="ACGE4H_001677"
     CDS             6894..7130
                     /gene="rpsR"
                     /locus_tag="ACGE4H_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFF8365090.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7273..9246
                     /locus_tag="ACGE4H_001678"
     CDS             7273..9246
                     /locus_tag="ACGE4H_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFF8365091.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9252..9704
                     /gene="rplI"
                     /locus_tag="ACGE4H_001679"
     CDS             9252..9704
                     /gene="rplI"
                     /locus_tag="ACGE4H_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFF8365092.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            9978..11345
                     /gene="dnaB"
                     /locus_tag="ACGE4H_001680"
     CDS             9978..11345
                     /gene="dnaB"
                     /locus_tag="ACGE4H_001680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFF8365093.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3979 a         2175 c         2642 g         3137 t
ORIGIN      
        1 tctaaatacg gaattcatga cgttgggcca aatgttgaaa gaagtcacag ttatcggtag
       61 tggcggacaa gcaaaatggt atttggcaga aaacactgtg ttagtcgatg gcgaaccaga
      121 aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt gaagttccag aagtaggaac
      181 tttttttatg gtaaaaaaag gaaacgacga taatgaggct gaataagctg tatttaaaaa
      241 attatcgaaa ttacgaagag ctgaacatcg aattttccaa gaacctcgtc atctttttag
      301 gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta gctatgacgc
      361 gaagtcatcg gacaagtagt gagcaggaat tgattggctg gactgacgat caggcgatga
      421 ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt agaaatctta ttatctaaaa
      481 aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa gttaagtact tacgtgggac
      541 aaatgaatgt cattttattt gcaccagaag atctttcttt agtaaaaggc agcccgcaga
      601 ccaggcggaa gtttttagat atggaattag gccagatcaa tccaatctat ctttatgatc
      661 ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa
      721 aaaaacaaac tgatttgctt tatttggata ttttgtccga acaactagca gcgtttggca
      781 gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg gctaattctt
      841 tgcatcagca aatcacccat caaaaagagc agctggaaat cgagtatctc accgcagtcg
      901 attctttaga aacacatacg caagagcaaa tccaagaaca atttctcgca cttttgaacc
      961 aaaataaaaa gaaagacctt tttcggggaa caacgacagt tggtcctcat cgtgacgatc
     1021 tttccttttt tattaatcag aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa
     1081 caactgcact aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag acaggggaat
     1141 atccaatcct tttgttagat gatgtgatga gcgagctgga tgataatcgt caactccatc
     1201 tgctagaaac aatcgaagga aaagttcaaa cgttcttaac aacaacgaca cttgaccatg
     1261 taaaaaataa aatgactgtt gaacctgaga ttttttatgt tcaacaaggg aaactaaaag
     1321 gagaagaaac tgaatgacag aagaaagaag tttagtagaa cgcgctaaag agtatgatgc
     1381 cagtcagatt caagtacttg aaggacttga agccgttcgt aaacgacctg gcatgtatat
     1441 cggttcgact agttctgagg gtctgcatca ccttgtttgg gaaattgtcg ataattcgat
     1501 cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa aagacaacag
     1561 tatcaccgtt attgacgacg gtcgggggat acctgtagat atccaggcaa aaactggacg
     1621 tcctgcagtt gaaacggtct ttactgtttt acatgctgga ggaaaattcg gcggtggtgg
     1681 gtacaaagtt tctggtggac ttcacggagt aggatcttct gttgttaacg cgctttctac
     1741 acagcttgat gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg
     1801 agctgtagtg gacgacttaa agattatcga acaaacggat cgccatggaa caacggttca
     1861 ctttacgcca gaccctgaaa tttttacaga gacaactgag tttgattttt caaaattggc
     1921 tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa
     1981 acgagaagaa gaaccagtca tcaatgaata ccattatgac ggcgggatta aaagttatgt
     2041 cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt tggaaggtga
     2101 acagcaagat atcgcagttg aagtctctat gcaatataca gacggctacc attccaatat
     2161 catgagtttt gccaacaata tccatacgta tgaaggcgga acacatgaat caggattcaa
     2221 gactgcttta actcgtgtga taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa
     2281 tgacgacaac ttgaccggtg aagacgtgcg agaaggattg acagctgtta tttctatcaa
     2341 gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg
     2401 gacagtcact gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag
     2461 tgtgggccgt caaattgttg aaaaaggatt actagcttcc agagctcgtc tagcagcgaa
     2521 acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag attagtaatc tgccaggaaa
     2581 attagcggac tgctctagca acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga
     2641 ttcggccggc ggatcagcta agcaaggtcg taatcgagag tttcaagcca tcttgcctat
     2701 tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac aagatcttgg caaatgaaga
     2761 gatccggtct ttatttactg ctatgggtac agggtttgga gaagattttg atgtatcgaa
     2821 agctcgctac cacaaattag ttatcatgac tgatgccgat gtcgacggcg cgcatatccg
     2881 tacattgctg ctgactcttt tttatcgtta catgcgtcct attgttgaag ctggatacgt
     2941 ctatattgct cagccacctt tatacggggt aaaacaaggg aagaatatta cgtatgtaca
     3001 accaggaaaa aatgcagaag aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc
     3061 aaaaccaagc gttcagcgtt ataaaggact tggagaaatg gatgatcatc aactatggga
     3121 aacaactatg gacccaagca atcggatgat ggcccgcgtc agtgtggacg atgctattgc
     3181 agcagatcag atatttgaaa tgttgatggg ggatcgagta gaaccccgtc gtgcattcat
     3241 cgaggaaaat gcacactatg tgaagaactt agatatttaa tcatgaagga ggaaaaagaa
     3301 tgagtgaaga aatcagagaa aacatccatg atgtcaatct aaccagcgaa atgaaagact
     3361 ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc acttcctgat gtcagagatg
     3421 gtctgaaacc cgttcatcgc cgtattttat acggcatgaa cgaattaggt gtaacaccgg
     3481 ataaagccca taaaaaatct gccagaatcg tcggagatgt catgggtaaa taccatcctc
     3541 atggagacta tgcgatctat gagtcaatgg tacggatggc acagccattc agctaccgtt
     3601 atatgctagt ggatggtcat ggaaactttg gttctgttga cggtgacggt gccgccgcta
     3661 tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt gatatcaata
     3721 agaacactgt tgatttccaa agcaactatg atgatacaga aaaagaacca gttgtactac
     3781 ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg gatcgccgta ggtatggcca
     3841 cgaatatccc acctcataac ttgtctgagg tagtagcggc tatcgatttg ctgatggata
     3901 atcctgatgt gacgaccaat gaattgatgg aagtattacc tggaccagat tttccaacag
     3961 gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt aagggttcta
     4021 ttaccgtacg tgcaaaagtt gagattactg agatgccaaa tggtaaagaa cggatcatcg
     4081 tgacagaatt gccttatatg gtcaataaag caaaattgat cgaacggatt tctgaactgc
     4141 acagagacaa gcgtatcgaa gggatcacag atttgcggga cgaatcttcc cgtgaaggta
     4201 tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg aataacttat
     4261 ataaattgac atccttgcaa aattcatttg gattcaatat gttggcgatc gaaaaaggtg
     4321 tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag
     4381 tcatcacaag acggacagtc tttgaaaaag aaaaagcaga agcaagggca cacattttag
     4441 aaggtttgcg tattgctttg gatcatatcg atgaaatcat tgcgattatc cgcgggtcta
     4501 aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc gatcgccagt
     4561 ctcaagctat tttagatatg cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg
     4621 aaaatgaata ccaagaactc ttaaaactga ttgaagattt aactgacatt ctggcaagac
     4681 cagaacgtgt tacagaaatc atcaagaatg aattagcaga cttgaatcaa cgatttggtg
     4741 ataaacgacg cacagaatta ctagtcggtg aggtcttaag tctggaagat gaagatttaa
     4801 tcgaagaaga agaaatcgtt attacattga caaacaatgg ttacatcaaa cgtttggcaa
     4861 ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca
     4921 atgacgactt cgtcaaaaat ctggtttctt gttctaccca tgatacattg ttattcttta
     4981 cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat tccagaatac gggcgcacag
     5041 ccaaaggaat accaattatc aatttactag gtatcgattc gagtgaaagc atccaggcaa
     5101 tcattgcggt gacgggagaa gcagaagaag gccactatct cttctttacg acgcgtcaag
     5161 gtacagtaaa acgtacttct gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg
     5221 caattggttt gaaagaagac gatgagctcg tcaatgtgct actgacagat ggtcaatcta
     5281 atatcattat tggaacacat aacggctact ctgtcacttt caaagaagaa gcagtccgcg
     5341 atatgggacg tacagcttcc ggggttagag gaatacgcct gcgtgaggaa gattatgtcg
     5401 ttggggcagc cttaatgaag gaagatcaag aagtattagt gatcacagaa aaaggttacg
     5461 gaaaacggac aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa
     5521 cggcgaacat cactgaaaaa aatggtccac tagctggatt gaccactgtt tctggagatg
     5581 aagacatcat gctgattaca gataaaggtg tcattatccg tttcaacgta tcgactgttt
     5641 ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa gatggaagct gatacgaaag
     5701 ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa gaacaaggat
     5761 cagaagaata gccatcaaga ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc
     5821 tggttattcc tgctggcttt tttgattagt tgaatttctc ttaaaatatt cgaaactgtt
     5881 cattctgtat tgcttttgac caaaatagag tgtataatca gagcttgtga gtagtctaat
     5941 taattagata actctccttg ctcttaggaa cctaagagcc gaaagtccac agggaggtga
     6001 atatcaatgg aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa cattgatgaa
     6061 gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct tgaaagataa cggagcagaa
     6121 gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt
     6181 gaaggtatct atcacatcgt taaagtatct tctccatcat cagcaaatgc tgtcaatgaa
     6241 tttgaccgtc ttgctaaaat caacgacgat atcattcgtc acatgattgt taaagaagaa
     6301 gcttaattgt tgtttcatgt gaaacaatag aaaacgatga gaggagatga ctattttgat
     6361 taataatgtt gtattagttg gacgtctgac gaaagatcca gatttgcgtt acacagcaag
     6421 tggtacggca gtcgcaacat ttactttggc tgtgaatcgt aattttacaa accaaagcgg
     6481 caatcgcgag gctgatttta tcaactgtgt gatttggcgc aaatcagcag aaactttagc
     6541 aaactatgct cgtaaaggaa cacttttagg tgttactgga cgtatccaaa ctcgttctta
     6601 tgataatcag caaggacaac gtgtatacgt gacagaagta gtagctgaca acttccaatt
     6661 gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg
     6721 tcaatcagct ggcggatttg gcggaaacaa taactctaat caaacatctc aatcatccaa
     6781 cggtatgcct gatttcgacc gtgatacttc tgacccattc ggctcaagtt caacgatcga
     6841 catttcagac gatgatttac cattctaaac gaatttgata ggagggaaat agaatggcac
     6901 aacaaagaag aggcggacgt aaacgtcgta aagtagacta cattgctgct aaccatattg
     6961 aatatatcga ttataaagat atcgaattac taaaaagatt tatttcagaa cgcggtaaaa
     7021 tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa
     7081 tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag cgaagactaa taaatcctag
     7141 gaattgaatg actgatcctt ttgatcagct attcaattcc tttttttctg taaaaataac
     7201 ctctattcgt ttagtcgctc gaatgacgaa agttatggta aaatcaaatt agtcatatat
     7261 gggaggaaaa ttatggaaaa taaaaacaat catttatccc cgaaacctct cttccttatc
     7321 ctgtttatat taatcgaagt attggtagtc ttaattttac catttcgttt tgttagcgct
     7381 tcggtcattc tattgatgaa tttatggggt gttcgctatt ggttgaaaat gaaacgttgg
     7441 cttattttaa ataaacgtga aaaaatccag caggcttcga ctgtagctga aaccaatcta
     7501 gcttatgtct cagaagtcat gcctgtagga gtgattgctt atcagccatc aagccaaaaa
     7561 atcgaatggc tcaatccatt agcattgaaa atcaaagatc aaagtggatt aacagaggaa
     7621 gaattgctag atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca
     7681 gataaaacat ttcagtttga tatggataca gtaaagggtg ttgtttattt taatgatatt
     7741 acccaaaacc agttgttaaa actccatcag acgaacagtc agccggtgat tggtatttta
     7801 tctattgata actatgcaga tgccattgat aaattggatg acaaagagat ttcttatttg
     7861 aactctttcg tcactacact agtttctgac tggatgaatg aataccatgt ctttttcaaa
     7921 agaatcaacg cagaacggtt tttcttcgta gcacagatga tcgatctgaa aaagatgatg
     7981 gataaaaaat ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct
     8041 atcaccctta gtatggggat tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa
     8101 gctcagaata atttagatgt tgctttagtc cgtggaggcg accaagttgt cgtgaaagag
     8161 gtaaaagaag gagcaaaacc tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc
     8221 acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc ggatttttga tgaaacaggc
     8281 gatgtgttta tcatggggca caagtttccg gatatggatg ctttaggctc tgcttttggt
     8341 gtcagttgtc tggcacgctt caaccagaaa aaagcttaca tcgttatcaa cgagaacgag
     8401 atcattccag atgtagaacg gtgtcttgaa gaaatccata agcatcctga attagaaatg
     8461 caactaatct ctccagaacg tgccatagag ctgaaaaaag aagaggacct cctcgtgatg
     8521 gtggattacc ataaaccttc tctatcgatc tctcagggcc tatatgagca atttgataag
     8581 attgtgatca ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc tctgctgact
     8641 tatatcgaat cttcagcttc ttcagcttca gagctagttt ctgagctgat ccaataccaa
     8701 agcagcaaaa caaatcgctt agatcgtttt gaggccacat tattgcttgc agggatggtt
     8761 gtagatacaa atagtttcaa tgtccgtaca actgcacgaa cttttgacgt tgccagctat
     8821 ttaaggtctt gtggagcaga cgcatcctta gtacaatatt tattgagctc ggatttagcg
     8881 acatacttgg agatcagcaa tttgattgct ggaagcgact atgtgacttc tgatattgtt
     8941 gtggcggcag ggacagacga aaaagagtat aatagtgtta tagcagcaaa aacagcagat
     9001 acattgcttt cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg gacagacggc
     9061 ttgattggga tcagtgccag aagtaccggc acgatcaacg tccaaatcat catggaatcc
     9121 ttaggtggcg gaggacactt cacaaacgca gctactcaac tggaaaatac gacagttgag
     9181 gaaacaaaac agaagctatt tgctgcaatc aaagaaaata tgaatcaaat atatgataag
     9241 gagtaatcca aatgaaagta atttttttac aagatgtaaa aggaaaaggt aaaaaagggg
     9301 acatcaaaga agttccagca ggttatgcac aaaattatct aatcaaaaat aacttggcaa
     9361 aagaagcaac gaaaagcagt atcagtcagt tagcaggaca aaagaaagca caagaaaaac
     9421 acgaagctga aatcttggaa gaagctaaaa agttaaaaga attcttggaa aaagaagata
     9481 ctgtagtcga attgaaggcg aaagcagggg aagacagccg tttgtttggt tcgatccctt
     9541 caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat caaattagat aaacgaaaaa
     9601 tcgaattaga aaatccgatc cgttcactag gctacacaaa cgtacctgtt aagcttcatc
     9661 atgaggtgac ggctacgatc aaagttcatg tcgtacaaga ataacaaatc ttgatagcga
     9721 tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa
     9781 cagctgcagc aatgaaagca aaagctgcaa atcgatttca gattgatttc ggtttctttt
     9841 ttgggaacag caaaatagca taaagaaaac gagtataata aaagagaact atttttttat
     9901 tacactttcg gtacgaatga tgacttttct ctcattttct tgtaaaatga agcaattgaa
     9961 gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg caaaatattg
    10021 aagctgaaca agcggtttta ggctctgttt ttcttgacgc agaaacgatc attgatgcca
    10081 tggagtacat agagccaaaa gacttttatc gtcgtggcca tcaaattatt tttgagacaa
    10141 tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg
    10201 aacaagccaa tttgttagaa gatgccggtg gtttaagtta tctctcagac ttggcattag
    10261 cagttccgac agcagcaaat atcgtctact atgcgaaaat cgttgaagaa aaatcattgc
    10321 tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa aggattcgag caaggagaag
    10381 aagttcaaac aattttagat gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa
    10441 atcgaagcgg ctttttatct attgctgaag tactgaactc cacgattgaa aatatcgatc
    10501 gactagctca aaacaatgaa gaaatcacag gtctaccaac tggctatcaa ggtctggata
    10561 aaatgacagc aggtttccag cccgaagaac taatcattct tgctgcacgt ccagcggtag
    10621 gtaaaacagc gtttgcattg aatattgctc aaaatgtagg aacaaaaaca gatcgttctg
    10681 ttgcgatatt tagtttggaa atgggggcag aatctcttgt aaatcggatg ctttgtgcag
    10741 aaggttcgat tgaggccagc catttacgta caggtcagct ttcggaagaa gaatggtcga
    10801 atctgatcgt tgcgatgggg agtctttctc gagcaaatat ttttatcgat gatacaccag
    10861 ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc
    10921 taggcttgat attgatcgat tatttgcagt tgattgaagg taacggtaaa gagaaccgtc
    10981 aacaagaagt atcagatatc tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac
    11041 ctgtcattgc tctttcacag ttatctcgtg gagtagaaca gcgacaagac aaacgaccag
    11101 tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc gtagccttct
    11161 tgtatcgaga tgattattat gaaagagaag gcggagatga cgatgatgga aacgagccaa
    11221 aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca cgaggaacag
    11281 ttgaactact gtttatcaaa gaatacaata agttttcgtc gctttcgcca agagaagaat
    11341 tttaaagaag aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg
    11401 agtaaccgca ggaacgacta gagtacttaa tttaaacaga tctccaaagg gattgtgaca
    11461 aaagtcttgt cacagtccct ttttccgaat aaacggtgtt caatcagctg acccttggta
    11521 ttagctcagc aaacgtcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
    11581 ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg tgttctcctg ccaactcctc
    11641 ggtcacaagt cacaatatca tcaaacatga gaagcagttt tttgaaattg ctccttgtga
    11701 ctcagagtta aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc
    11761 tgcggtaata cgcccaatca aacgaaaaat atgagaagct atttttgttt ggctgttctt
    11821 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc aaaagtagct
    11881 tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa gcaattttcg tac
//
LOCUS       DAXWLB010000080        11929 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXWLB010000080 DAXWLB010000000
VERSION     DAXWLB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.080"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..258
                     /locus_tag="ACGE4H_001681"
     CDS             <1..258
                     /locus_tag="ACGE4H_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365094.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..665
                     /locus_tag="ACGE4H_001682"
                     /pseudo
     CDS             465..665
                     /locus_tag="ACGE4H_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1377..1982)
                     /locus_tag="ACGE4H_001683"
     CDS             complement(1377..1982)
                     /locus_tag="ACGE4H_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8365095.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2164..2499
                     /locus_tag="ACGE4H_001684"
                     /pseudo
     CDS             2164..2499
                     /locus_tag="ACGE4H_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2664..2879
                     /locus_tag="ACGE4H_001685"
                     /pseudo
     CDS             2664..2879
                     /locus_tag="ACGE4H_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3017..3988
                     /locus_tag="ACGE4H_001686"
     CDS             3017..3988
                     /locus_tag="ACGE4H_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8365096.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4128..5441
                     /locus_tag="ACGE4H_001687"
     CDS             4128..5441
                     /locus_tag="ACGE4H_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFF8365097.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5484..6350
                     /locus_tag="ACGE4H_001688"
     CDS             5484..6350
                     /locus_tag="ACGE4H_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365098.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6347..7195
                     /locus_tag="ACGE4H_001689"
     CDS             6347..7195
                     /locus_tag="ACGE4H_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365099.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7209..8681
                     /locus_tag="ACGE4H_001690"
     CDS             7209..8681
                     /locus_tag="ACGE4H_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8365100.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8698..9570
                     /gene="scrK"
                     /locus_tag="ACGE4H_001691"
     CDS             8698..9570
                     /gene="scrK"
                     /locus_tag="ACGE4H_001691"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFF8365101.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9806..11188)
                     /locus_tag="ACGE4H_001692"
                     /pseudo
     CDS             complement(9806..11188)
                     /locus_tag="ACGE4H_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11650..>11929
                     /locus_tag="ACGE4H_001693"
     CDS             11650..>11929
                     /locus_tag="ACGE4H_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFF8365102.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2037 c         2401 g         3791 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgaaacat ttgaagtgat cctagataaa attaaacaga
      661 attgatcaga aacaagcgaa gccttctctc ggttcgcttg ttttgctatt tattagttgg
      721 ctcagggcaa ctctaagctc gtttgaaaga aaaccgatgg ataatcccct tgtttgtacc
      781 actttagaat acatggatgt acgtattgcc attgactcct attagttact gaaaaaaaat
      841 ttaaacaagt tgagtattgc agataaatag ctccagtgag gtgaataact gctccactca
      901 aggcaataga tagctccatc cagggcaaca aactgctcca ctttcatatt taatagatga
      961 ataaccttct gtataataga aaggcatacc tttcggtatg acaacttata cagaatgtta
     1021 ttttattggt tcactatcga aaaattttgg agctttattt ttccggtatt tctcaacgaa
     1081 cgattagttt aactgtggga agctccagac atacgattcg agaagttact tctcgagcag
     1141 acaggaaggg ggtgactgaa ctaaccgctg atatgagcga tacttggctg taggaatatc
     1201 tttttccgga aaaacaaaca acagcacgag gttaccttcc tgtagactgg gaatatgttc
     1261 ataaagaact aatgaaaaag aatatgacat tgaaactcct tcatagagaa tatggggtac
     1321 atggaacata gaaacgaaaa tgcacggtag aaaagcgaat gaatatagta taaattctac
     1381 aaatcattcg ctttctttat tttatatatg gttggtcgag agagaccatt ttctttggca
     1441 atttctgcaa ctggaactcc ttgatttaac atgtccacca cagttttaaa gactaatcgt
     1501 ttttgcggat ctgttgagtt ctcagaatat tcaattggtc gccccttata tcgaccagct
     1561 tttttggcca ataatactcc ttgccgctga cgttcacgga ttttctcacg ttcgttttgt
     1621 gcaatataac ttagcaatga taaaaacata tcgaacatag cagtatctag gagttcattt
     1681 ccagtattaa aatctaaaaa ctgtgcatct agtattttta atgctacttt tttctgtttc
     1741 ataaaggtaa ctgtattttt tatgtcttca taatctctgc ctaggcgatc cagtgatgca
     1801 acaatgacac aatcaccttc tcggatataa tttagtaatt tttgaaactc aggtcgctgg
     1861 atatctttac cactggcttt ttcttggaat aatttatcaa tgttaaggtc ttccatcatt
     1921 gccaattgtc gatctaaatt ttgatcttgt gaagacactc taatatatcc aatttttgcc
     1981 ataaaaactc cttttaaaaa atgatataat ttacatgtaa aatttacttg tagttttact
     2041 gttatcatag catttttaaa aataaatttt aagtctactt ataatttact ttggaggttt
     2101 tgaatgaatg aaattaagcg caataaaaat tcttttggtg tttatttagg agagcctagc
     2161 tcggaacagt taaataaagg tgaagctcgt catgctctat gtcgaaatat attttatggt
     2221 agaaaaggca aactttatca aacttattac gacggtatgg aggaacagct caacgcatta
     2281 ggattagtaa ctaatgctat catatattgg aattcactat atttagaaaa agtctttgaa
     2341 caaattaaaa cggaagggtt cgactgttcc gaagaagtgt tacgcaagca atttccatta
     2401 attagtgatc acattaattt tgttggcaaa tatagtttcc gctatgaaaa agaattatcg
     2461 aatggaatac tacgtccgtt aaatatagag aattattgag aatgttcgtc ttttggttcc
     2521 taccgtgcat tttcgcacgg atgttccaga tactattttt gaatgtataa aattgaaaac
     2581 acaagaatac taataaatca acgattcttg tgtttttaat ttttcactac cgtgcatttt
     2641 cgtttctatg ttccatgtac cccaatcttg aaaaattaat taaagactta gataaagcca
     2701 ttgacgactt agtcgtggtg ctaccggagt atcaatgttt aaccagtatc ccaggtattg
     2761 gtcctgttta ggctgctgga atactcgctg aaattggtcc aattgaacgt ttttctgatg
     2821 aaataaaatt gacaaagtat gccggcctat attggcgaca gagtcaatcc ggtaaatctg
     2881 aatacgaaaa tttttttacg aaaagcttca cgtctaatat ctattttaaa ataaaaatat
     2941 tcctgctaat ttggaatcgt ttccttcttt tggtataata atacctgaaa ttcttttgag
     3001 aggagttgtt cgtgaagtga aaactactat gaaggatgtt gctgcgttag caggagttgg
     3061 tgtgggtacg gtatctagag taataaatgg agtaaaggtg aaagaggtta ccaaagaaaa
     3121 agtaatggct gcaattagtg agcttaatta tgagcctgat gaatatgcca gaggtatgaa
     3181 aacgaatagt agcaatacaa tagctcttat tttacctacg atttggcatc ccttcttttc
     3241 cgagtttgcc tactatgtag aggaagctct cagccaaaaa aattataagc tttttttgtg
     3301 taatgcgaac ggcagtgcgg aaaaagaaaa agaatatatc caaatggtca agcagagtaa
     3361 agtagacgga attataggta ttacctattc agacattgat aaatatgtat catcaaattt
     3421 accatttgtc agtatagacc gccatttttc ggaacaggtt tcttatgtaa cggcagataa
     3481 ttatttaggt gggcaaattg ccgctcaaga actacttact cgtaactgta aaaacttgtg
     3541 ctatgttggg ggtgtttcac cttaccctaa cgaaacaaac aatcgtaaag ctggttttta
     3601 caatatttgt gaggaaaata aaatagctgt gaaggtattg gatatgcctg aaccaattag
     3661 caatttagag gaacaattgc tcgctttttt ttcggataat cctgaaattg atagcatttt
     3721 taccatcaat gatttcatgg ctttggatgt gataaaagcc caaaaaaaac tagggaagga
     3781 agcacctaag gattttcaaa ttattggttt tgatggagtc aagctatcgt tgcaaaaaga
     3841 ctatttacta tcaacgattg tacagccgat agaggaaatg gccaaggttt cagtagaaac
     3901 actcatggcg atgattttaa atgaagaacc gcctaagcgt gtggtagtac cggttttctt
     3961 tcacgctgga gagacgacta aagagtaaaa aaccggttta taacgatttt tttgagatag
     4021 gttgacagaa agtgatttca atgatatctt aaaaccaatt aattggaaac gttaccaatt
     4081 aattgaaaaa taaaacggaa acgtttcaaa aaataggagg aacatcgatg aaaaagagaa
     4141 tgttagcaat tgcagcaatt ttagcattaa cagctggagg acttgcagga tgtggcagta
     4201 aggagaaaag ttcaggtagc agtgatccag gagaaattaa gatttgggtt cagttttctg
     4261 atgaaacgcc tgaaggaaaa gcttgggcag agattgtgaa caattttaat gatgaatacg
     4321 agggtgaata taaagctgtt actgagtata ttcctcgtag cggaagcggc ggtggatatg
     4381 aggataaagt aaatgctgca attacaacca atacccttcc tgatgttatt acgttagatg
     4441 gaccgaatac tgcagcttat gctcattcag gagtcattgc tccattagat gattatttga
     4501 aagatgcgga catggatgat gtattagaca gcattattca gcaaggaacc tacgatggca
     4561 aaatgtatgc gtttggtttc tcagagtcca gtgtgggtgt ctattacaat aagtcgctgt
     4621 tcaaagatgc tggaattaca gaagatcaat tgccgacgtt agaagatcct tggacttgga
     4681 cggaatttca agcaatttgc caaaagctga ctgataaata caaaacgcct gcaattgata
     4741 tgcaaatggc gtcgggagac gaaatgctga cctacgcata tacaccattt atatggtcga
     4801 atggcggcga tgtcgttaat gctgatggaa ccaaagccga agactatttt aacggtaaaa
     4861 aatccgttga agcgttggaa tttattcaag aattggttga aaaaggttac accacaatca
     4921 gtcctattga aaaaggattt gaaactggca aatatgccat gatgctgagc ggttcttgga
     4981 caatggcaga tttagaggaa aactatcaag agattgatta tggtattttg ccttaccccg
     5041 tttcagatgc aactggggag ttagtttcac caaccggaag ctggcagtta gcaatgactg
     5101 agaaaacaga gaaaaaggat gcttctgcgg cctttattaa gtttgcaacc agtacagaat
     5161 ctagtcgtat tatgagttta gggaacagtg ttctacctat cagaaattca acagttgatt
     5221 taattaaaga tgaagtgtcg gaaggaatgc aggtattaat taagcaaaac caactttctg
     5281 gacatgcccg cccagtggtt gtagcttatc cgcaagtatc tagagcattc caacaagcag
     5341 ttcaagatat tagctacttc gaggaaaacc cagatgtaca aaaagtagca gatagtcgcg
     5401 ctgctgaaat gcaacaggca attgatactt cactaaaata gaagtttcta aagggggcca
     5461 atcaagtgtt aaaaaaaccg acaatgaaag acaatttgat aggttacggc tttctagcgc
     5521 cagctttgct gctgctgacg atttttctga tcatacccgt aatcatggta atttattatg
     5581 cctttactga ttattatttg ttaacgccag atgctagaca atttatcggc ttagaaaatt
     5641 ttaagcgact gttcgctgat ccaatttttg taaaaagtat ttggaacacg gctaaatttg
     5701 ttgtttggat tattccggtc caaatagggg cagctttagg aatggcactt attatcaaca
     5761 aaaatcgtaa aggaaatatc ttttttaaag tagccttttt tgcgccagtc gtgatgtcct
     5821 tagtcgttat ttcgatttta tggttgtact tgttaaatcc taatgaaggt ctggttaata
     5881 cgctatttgc aaagattgga attgcttcgc agcctttttt gactagtccc aaacaagcaa
     5941 tgtatacgct tgttttcgta tcggcatggc aaggtgcagg atatcagatg ctgctgtttt
     6001 tagggggaat gcaaaatatt ccgcaagacg tttatgaggc agccgaaatt gatggttttt
     6061 ctaagtttca gcaatttcgc tatatcacca tgccattatt aaagcccacg gcgattttta
     6121 ttttacttac cacattgatt tcggccttta aactaatcat tcagccaatg gtaatgactc
     6181 aaggtggacc aatgaattct actatgacaa tggtttatta catttaccaa actggattta
     6241 cagaccgtat ggtaggctac tccagctcca ttgcgttaat ctttaccact atgattggtt
     6301 taattacaat tgctcaaaga aaattggtaa aggaggatga tccagcatga ccaagaaatt
     6361 tggaccgcta atgatcgtag aatattgttt gctgttcctt ttagcggcat tattcatttt
     6421 cccgatgctg tggatggtag tttcctctat gaagcctgaa gcagatgtct atcaaaattt
     6481 agcttccttc aaagcttttt tacctagttt gaatccagct aattggttta aaacttatca
     6541 agaggttatt tcccggttca gtgtgggaac ttatttatta aacagtattt tttatggttt
     6601 aacctttgcc tgtgggtcaa tcattgtaaa ctctttggcg ggttttgcct ttgcgaaggt
     6661 taatttttca ggcaagaaaa ttatctttgg agctttgtta gctctgctga ttgtcccgat
     6721 ggagacggtt ttaattccgc aatttaccat cgttaattca ctaggcttag tgaatactcg
     6781 cttggcagtg attttaccag cgatggccag cgcgtttaat atctatctgt ttagaaattt
     6841 ctttatcgcg attccagaag aaatcattga atcagcgaaa ttagatggtg caaatattgt
     6901 taaaattttc tttcacatca tgctgccaat gtctaaacca gccatagcca cagtaggtgt
     6961 cttatccttt atctccagct ggaatgatta tatttggccg ttaatggtct taactgacag
     7021 cagcaaattt tcaatgcaag tggcaattac tacgatcaat actacacagc cagtttatat
     7081 caatcaagtg atggctgttt taaccattgc aacgattcca ctgattattg tttatatcgt
     7141 tgcacaaaaa tatattctgc agggacttgg cggttctgga acgggtataa aataggagtg
     7201 aatagtgaat gttgcaaaaa cttttaaagg ccaatgagtt tattgagaaa aatgctcaaa
     7261 cagtcaatca agaataccgg aataaatttc atttgatggc tccgattggc tggatgaatg
     7321 atccaaatgg ttttgtttat tatcaagagg agtatcactt gttttatcaa tattatcctt
     7381 acgacagcat ctggggacca atgcattggg gccatgctaa aagcaaggat ctaattcact
     7441 gggagcattt gccagtagca ctggcaccag gtgaaagcta tgacaaggaa ggctgttttt
     7501 ccggcagtgc catagaaaag gatggcaagc tgtatttgat ttatacagga cacaatgttg
     7561 tggaagggca agttcgacaa gttcagtgtt tggcagtttc agaagatggc attcactttg
     7621 aaaaatatgc cggaaatcca atcatcgcag aagatcactt agcttctgtt gcaacgaccg
     7681 aagatttccg cgatcccaaa gtgtttcagc gaaaagaaga ctattttact gtagtcgcta
     7741 ctaaaactgc agatgctcgt ggacaaattg tgatgttcca ttccaaagat ttactaactt
     7801 gggagtttta ttccgtattg ctggagggaa ctccagaaca aggcattgta tgggaatgcc
     7861 cagacttgtt tgaactagat ggcaaagaag tcttgattct ttctcctatt caaatgactc
     7921 cacaaggcaa tgcttaccaa aatacgaact caactgttgc tttcatcggt actgtcgatt
     7981 ggcaaacagg caaatttcta gtagagaatt ttcatgaaat cgatggcggt ttagattttt
     8041 atgcacccca gacctgtgaa aacgaagcag gccaaagaat aatgattgct tggatgcaaa
     8101 tgtggcagcg taaaatacct acgaatgatc tgggccatgg ttggtcaggt tccatgacct
     8161 tgcctagaga gctgagggtg gctgataatc aattgttgca acggccagtt tcatcaattt
     8221 atcaagtaat tgatccgcag gaaaggattg aatgggcgaa gactgaagag aatcgcttct
     8281 tactgaagga cttagtatat gaccagcact atttacgctt ggaaatcaat ttaaaacagg
     8341 caacgaaact agaacttcac tatgcgaagg gacccgacca tacattactg cttaactatg
     8401 atgttgaacg ggagtgcttt actgcttcca gggaacacat gccgtatgat ttatcaggag
     8461 ctgaaaagga agtgttgaaa aagcgcagtg tgacgattcc tttggaaaat aattgtctaa
     8521 ccatcgaaat tttccgtgat acttcagcta ttgagctttt tctaaatgat cgtctgacta
     8581 tgagtacaac tttttatgag acagaaaaag gacaggattt tcttttgctg gcagacagtg
     8641 aagtaacctt ttcattggaa agcggcttaa ttaatctata gaagggagag ctcagtgatg
     8701 atttatggtt caatcgaagc aggcggcaca aaatttgtgt gtgccattag cgatgaagaa
     8761 atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac aataccttta
     8821 gtcattgact tcttcaaaca ataccaagca gatttagcag ggattgggat tggctcattt
     8881 gggcctatcg atatccatcg cgattccgcc acttatgggt acattacctc gaccccgaaa
     8941 gtggcatggc aaaattttga tttcatcggt accatgaaaa aagaatttcc gatcccgatt
     9001 tcttggacaa cagatgtcaa tgcggctgcc tatggtgagt atgtcttcgg cagtggcaaa
     9061 ggattgagca gtgtcgttta ctatacgatc ggtacgggtg ttggcggcgg tgccttacaa
     9121 gaagggcgct ttatcgaagg gtttagtcat ccagaaatgg ggcatatgct cgtcgtccct
     9181 catccaaaag atgactttgc tggcagctgt ccatttcatg gcaactgttt agaagggatg
     9241 gccgctggac ccgccatcga aaaacgctta gggaaaaaag gacaagaggt tgcagaagat
     9301 gatccttatt gggaaatcga agcctcctat attgctcaat gtgcctacaa tacaaccttg
     9361 atgttttctc cagatgtgat catttttggc ggtggtgtga tgaaacaacg ccatatgctg
     9421 caaaacgtcc acgacgcatt tgcaaaattg gtcaatgggt atgtcgaaac acctgagtta
     9481 agcaaatata tcgtgacccc agcattagaa gacaacgctg gaacgctcgg ttgtttggca
     9541 ttggctcgag aagcggtctt acattcataa acgaaaaaag gctcttcgac atttagtgtt
     9601 ggtagagaaa gatacaactg aaaatttagt cataaccagt taggcagagt gcttaactgg
     9661 tttttgtttt acttggaaga tcaagccttg aatgagataa gttcttgctc gaaagttatg
     9721 gaaatttcga tagccgtagg ctacttgtat tgtgcaatag ttttttacaa aaaaaagggg
     9781 gtgcggatat tttttttgga cctttttaac aaatatatct ttgcttcttc cgatattgaa
     9841 gagggcttag ccttcctaaa gataacttaa ttcgttgatt attataccaa tgaatataat
     9901 catccaattt gtttataaac tgattgattg ttgtattctt ccagtcacgt tgataaaaca
     9961 tctcgttttt taatcgtcca aagaaacctt cgcacgctga attatctggt gaataactct
    10021 ttttagacat agagtgaagc aagttggctt tgttccaata gtccaactaa caatagcacc
    10081 atcgtaacaa tcgacaagtg gtgataagta gattttccct gcgagaattc taaattcagt
    10141 aatatctgtt aaccattttt catttggctt ctttgcttta aaattccgat ttaataaatt
    10201 ggggtgtgca ggagaaattt ccccagcata cgaagaatac ttttttcgtc tagaaaattt
    10261 aacaaataga ttttcctcag tcatcaatct acgtacaact ttttcagaaa gctttttacc
    10321 catatttttt aaagcttggt gaattctacg gtaaccatac gcacagaagt tatgattgaa
    10381 aatcgtgata attaatttga cgttctttgt aatacttatc tggtttctca agtgcttgtt
    10441 tttgataaaa atagctgctt ttaggaaact ggagctgttg aagaagctct ttcaaggtga
    10501 actgatttcg taaggcatca atcaatatcg ttttttcttg atttgataga caaagaagat
    10561 tgacgccttt ttcttttttt agcagttcag ccgctccttc gagtaatgct ttttcgagct
    10621 gtaattggtg tacttgtttt tgtaagtctt ctacttcctt taaaagtagc tctttttgtt
    10681 tttcctgatt ttttaaagtc atacacaatg gaaaatcctt tccaagaacc gtgtttttcc
    10741 attgatatag agtaactcta ctaattcctc cagattcggc taaatttttc ctattagttt
    10801 gcggtttata gaattttttc aacacagctt ctttttgatc ttgtgttaac tttaccgcac
    10861 tttttcggag ttttctagct cctggtgcaa gttcttcaca ccgctgccgc aaggactctc
    10921 tattaggata accaagcatg cgaatggttc gtgcataaca ttgaccatat tcaaaataat
    10981 gattcaccgc tattcgtttt tgttcttcag aatactttga tttttttcgg tgctcctgat
    11041 ggagctctcc ggattccaaa tactctttat accatttccg tagtgcacca atcgacggat
    11101 accctaattc tcgaatggtt gcagcataag agcaatcgta ttttaaaaat aactgaattg
    11161 cccttacacg ctctttgtgg gaaaacacac ggacattcct cctaagttta ttttttcgtc
    11221 caggattatg tccgcacccc cttgctttct gatgacttat tctggttctt tgagtgtcaa
    11281 tgcatagatg tgatgtagct agttagaaaa aaattttgaa tactcttttt ttctttcaga
    11341 ctccttttta agtaagattt tgaagtgtga gttacggggt cagaaactta aaagtttttg
    11401 atcctttatt ttcaagtctg atttggcagc ttttcggcag ctcatttatt tatggtcaga
    11461 aatagatgct gaaattttaa tctattagca aatagtccag aacgtatgaa gaggagtcgg
    11521 atatcatttg tcattaacac cataaatatt tataaaattc gtgttcacct acaattaaat
    11581 ttaatatata atattcgttg taagggctta taattttttg ttaatgaaca agataggaag
    11641 aaacatttaa tggacagttt tcagagattg tcactagtaa atcaatttga gttattggct
    11701 aaatttgagg accaaaacag atcttattat gaaagaaaaa tcgaaatatt gagagaaggt
    11761 tacgagtatc attatgatga tgaaatttgg agtgatcttt ctgagccatt tccgaaagaa
    11821 gattctcgat ttgtattaga tgtattgaac atgtatcgag atatcaattt tagtaaatca
    11881 aagctaaact tagatgaaaa ccaaattact aattcatact atactcatt
//
LOCUS       DAXWLB010000081        11837 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXWLB010000081 DAXWLB010000000
VERSION     DAXWLB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.081"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(559..1599)
                     /locus_tag="ACGE4H_001694"
     CDS             complement(559..1599)
                     /locus_tag="ACGE4H_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8365103.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1948..2274)
                     /locus_tag="ACGE4H_001695"
     CDS             complement(1948..2274)
                     /locus_tag="ACGE4H_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365104.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2261..3049)
                     /locus_tag="ACGE4H_001696"
     CDS             complement(2261..3049)
                     /locus_tag="ACGE4H_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8365105.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3417..3707)
                     /locus_tag="ACGE4H_001697"
     CDS             complement(3417..3707)
                     /locus_tag="ACGE4H_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8365106.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3843..4022)
                     /locus_tag="ACGE4H_001698"
     CDS             complement(3843..4022)
                     /locus_tag="ACGE4H_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365107.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4019..4369)
                     /locus_tag="ACGE4H_001699"
     CDS             complement(4019..4369)
                     /locus_tag="ACGE4H_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365108.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4362..5687)
                     /locus_tag="ACGE4H_001700"
     CDS             complement(4362..5687)
                     /locus_tag="ACGE4H_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFF8365109.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6137..6751
                     /locus_tag="ACGE4H_001701"
     CDS             6137..6751
                     /locus_tag="ACGE4H_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8365110.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6916..7158)
                     /locus_tag="ACGE4H_001702"
                     /pseudo
     CDS             complement(6916..7158)
                     /locus_tag="ACGE4H_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7362..8681)
                     /locus_tag="ACGE4H_001703"
     CDS             complement(7362..8681)
                     /locus_tag="ACGE4H_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFF8365111.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8815..9423)
                     /locus_tag="ACGE4H_001704"
     CDS             complement(8815..9423)
                     /locus_tag="ACGE4H_001704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365112.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10061..10318)
                     /locus_tag="ACGE4H_001705"
     CDS             complement(10061..10318)
                     /locus_tag="ACGE4H_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8365113.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10311..10580)
                     /locus_tag="ACGE4H_001706"
     CDS             complement(10311..10580)
                     /locus_tag="ACGE4H_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8365114.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10711..10890)
                     /locus_tag="ACGE4H_001707"
     CDS             complement(10711..10890)
                     /locus_tag="ACGE4H_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365115.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            10988..11224
                     /locus_tag="ACGE4H_001708"
     CDS             10988..11224
                     /locus_tag="ACGE4H_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365116.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(11271..>11837)
                     /locus_tag="ACGE4H_001709"
     CDS             complement(11271..>11837)
                     /locus_tag="ACGE4H_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365117.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3603 a         2163 c         1789 g         4282 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 tgtattttca attatcacaa tttatcgtcc aaaagtgcag aacgaaaatc aattcatcac
      181 aagcatatca gacaattaaa tttaaaacaa ctaaaaatat acaaaaaagt gtaattgaat
      241 attagcagca ctgcctgaaa agaagtaata tcaggctttt cagcttgggg aaataagtga
      301 attacctctc ttcatgaatt attataactc aaaaaagcac atatgcaaac actagttccc
      361 tatttttttg aatgaaaaat aactaaaata tctaacgatt atgactttaa aacctacgtt
      421 tgtgattgtt ttcataaact tttaatctaa aaactccgga ataatagcta tttatttgta
      481 acatttgtaa taaaattaaa atcataaaaa agttctaatt taaaattgat catttttata
      541 gtgatcgctt tttatcaact actctagcca attttccgta ctaactgttg gtaattcgtt
      601 aaatgtttct ctttgttcgt ttgcaagctt tgccgcaata gctttttcga caaaccaatt
      661 ttttacataa acgaaaatta gattctgcaa attgtttact ttttcagttt tttgtttttg
      721 ataagctttc agcaaagtca tatataatcc tttgtcagca tcaattccat aagattccag
      781 ttcttcataa acaacaactg tttgctctaa ttcttcagct tttttctttg cattatgaat
      841 agtttggacg gttttttgag cgtcttcgta agtactagaa aatgttttta tcaagtttaa
      901 tacattttct ggtacaaatg tatttatact gtcctctttc atgatgtcga cgaagttatc
      961 tagtaaaact tgattttgta actgatcagt ttcagtgtct attatgtgtc tattagtgtc
     1021 tatagaatta gaatagaata gattgtctgc gattttcgca attacggttg agtccttgat
     1081 atgactggat tttgccgtat ttgcgatttt cgcaattacg gtggtatcat tgatttctcg
     1141 cggttttgcc gtatttgcga ttttcgcaat tacggtggta tcagtattgt cttcctcttc
     1201 catgatttta aagacgtctt gattcgttac ttctggcttg cctaagtaca ataaattagg
     1261 catggattca atcttaccgt cacgtttatt tactcttcct ctcttctgtt tcagaagacc
     1321 tgctttttct aattcctttt tgattttaga tactttacct tcgcggcatt gtaacaattt
     1381 cattaattct tcaactgtga atatgaaaaa tatattatca tcagaatcta cccagttatt
     1441 tttgactgaa taatcaaatc gatctttaag tagcatataa gcaatttttg catcatttga
     1501 tagatccatg tatttttcgt tagtaaaaaa taccttaggc atttgataat atttttcacg
     1561 ataaatttct tttgatttgt aataattaaa atcattcata gccaatcctc tttctgtagg
     1621 atagcgtttt gttcaaaact atgttacaat gaattaaaca aatatatcta tccactatat
     1681 atttttctac tgctgataag tcgccaaact ttgttaaagc agtagtcttt ttttatcttt
     1741 ttttagttta ccttaaaaaa taaattaaag caaaccgctc tatttcaagg tttaagctca
     1801 tagtaactat atcacgatat tacaataata ttatatcacg atatcgtaat attacaatat
     1861 gaagataagc gacttatcag ctttgatact actgattttt ataaaaaaaa tatcacgata
     1921 ttatgatatc gtgatattga atgtaaatta aattttattt tgtttctttg ctagtttata
     1981 tccttcaatc aagtattctt caggtatgga ctttaatagc gcatcttcta taacgtctgg
     2041 tttacttttg tctgattcag caatagtcgt ccaatatttg agtgctgaca ttagaattgg
     2101 atttatacga atgctatctg ctgttcttac actacttcct tgatctttta tgactttact
     2161 tatatttttc ttggttaatt cgatttcttc tacttctttt gatttaaaat tctttttatt
     2221 tttatttcct agtaatctac ttactgcagc ttgactgcga ttatcttctg ccatcaaaat
     2281 caccaacttc ttttaaatat tcttctgtaa atgctttata gtcatcttca gctgttgagg
     2341 gtttattaaa attgattaga tctgatccag cagcttgcgc ttcgtcaacg gttattgtcc
     2401 tacgaatata agttttgaaa atttttgcac caatgactct agaaatttcg gttgctgcag
     2461 acgtcatttc tttagaaatg ttataacgct tattatactt agtaaataga actcctcgta
     2521 cttcaagctt gtcgttcgta tattctttta ccgtttcaat agtttcactt aactttatta
     2581 gtccttttaa tgagaaatac gcagcttcaa ctggaatgat aatttcatca gaaacagtaa
     2641 atgcgttgac tgtaagcatt cctaaacttg gtggacaatc aattataata taatcatagt
     2701 tgtccttgat tggttgaagt actcttttaa gcctgtgttc acttcctact tgagtaaatt
     2761 ccctctctgc agaagaaagg gctatgtcgg ctggcaaaac atcaattcct ttatagtcca
     2821 gaatagcatc tgtaatgtca gcctcccctt ttaatacttc ataaattgta ggttggtttg
     2881 ttccatctat atcgaaacct acattatctg tagcatttcc ctgaggatca atgtccacta
     2941 aaagtacatt tttaccgtaa acaaatttca aacatgctgc taaattaacg ctagtcgtag
     3001 ttttagaaac tccacctttt tgattggcta ctgacaatat tcttgccatt aaaatatccc
     3061 ccattcatct tatattataa tataattata tcataatatt gtgatattgc aatatcgtat
     3121 atgcattata ttgtaatatt gtgtgattat tttgttttaa agatatatat cggttcggat
     3181 ataaagatta ttcatatgtt attcgaaatt aaaataatca ctttttatta ctttaaaatt
     3241 ttttattaat gataagaaat atgttttgcg agcagcaact agaataattt aaacattact
     3301 aaactgtcta tattgttact aataattccc ttcttatagt gcattttctt agaaaaaaac
     3361 aatattacga tatcatgata ttaagatatc gtaatattat acaattgtga ttgatattat
     3421 atatgttttt ttattttttt atcatgttta atcttgaagg aatcatattc tttttctaaa
     3481 gacaggagct tttcataaac atcagacatg tttaaactag aggccaggct taaatcataa
     3541 acgaatgaag caggtaatga ttttattcta cgttgtcggg ttatccagct tgcaaccgtt
     3601 ccttgtgggt aggcatataa ctcacaaaat tgttctacag tgattcctaa attcttgata
     3661 atgtaaacat tgatcgggtg cgagtaaaca aatgtattga tagacatggt gcgtaagcct
     3721 actttctttt attattgcgt ttgcaataat agtttaacac acgcattatt ctagtatcta
     3781 ctcctatttg taaaaacaaa aaaggattca tagagataac tctatagaat ccgtaaaata
     3841 ccttatatat ataacgggaa gatataaaac ctaaaattat gataacctgc tgcagcttct
     3901 acatagaaag agaagcgctc aacaccaacc tcatttatca aacgttggcc ataaccccaa
     3961 atctcttctg atagctgatc aaaggttata tactttagct catattgctg gataatgttc
     4021 attctaatcg actccattta ttatcctggt aaaattcgat atgacgtatt tcgtcgtagt
     4081 gaattttgtc ttttccaacg tagatcccca gctcatcact acctaagatc ttgccaatga
     4141 tatcttctgg atagtatcct tcagcgtttc gttcttcttt ttgaatagat actgatctat
     4201 tttttatgac tgcagcgttc aatacttccg caatctcttc agtcgtcatt tgttcttttg
     4261 ctaaattttg actggttcgt tgttcattgt cggcatcaat ttcttcagta tgatcagata
     4321 aataaaaccc tgcccacttt aaacgtccac gatcattgta atcaatcatt tttaattccc
     4381 ccatttccgc ccgcatgacc accaacaagt gtgcttctag taatgcttct agcgccttct
     4441 aacattgaag aagcatgtac aatcgacgta aaaccatatt tttgccgaat cttgtcaata
     4501 attttatcta aattttcttc gtcaatttgc ttttgtggat cactaaataa atccagctga
     4561 agcgaatcgg tgtacacaag cttgctatag gttattccaa catgccttac ttcttgtcct
     4621 tcataatact tatcaaacaa gaaaagacaa taattctgca attcctttgt attatctgtg
     4681 atagggatct tcatctggtg tgaaaaccct ggtctcgttt ctaagatcga cgtgccaatg
     4741 tgtaaatgga cacaacctgt tttacagtta tgctgcctaa tgcgtatcgc tacttgttcc
     4801 gccatttctt ttaccaccag ctcgatttca tttcttctag tataatctcg tggtaacact
     4861 tgtgaatttc catatgattt ttcctttgtc ggttctggtg ggattgctat atctgttcga
     4921 tcgatcccat tagcatggaa gtacagctgt aagccaatca cacctagtct attcttaatc
     4981 gtataaggat tccagttagc aagatctcta atgctcataa tccccatctg attgagtctt
     5041 tttttcattc gagagccaat tccccaaaag tccgtcattt caggaatatt ccatactttt
     5101 tcaggaacgt tttcgtacgt ccattccgca acaaatcctt cattatgctt tgcttcgttg
     5161 tctaaggcta gtttagccaa aaggggatta tctccgacac ctactgtagc aatcaatcct
     5221 agctcttctt taatattctc ctggatcatt tgagccagct tctttctccg ttgacttcgt
     5281 gttccttcag tcgtaaaaag attcagtgat cgggtcactt ttaagattga ttcatcgatc
     5341 gagtaaatca gtagatcttc atcagccaca tatcttctga aaatattatt tacctgcata
     5401 tttcgcttga tatataggtt catacgtggt ggaacaacgt gtagtgtttt aggaaatggt
     5461 tgtggtaagt cacgtggtct actcacattt gtaataccat accgcttttt tgcttcagga
     5521 gaagaagcta atatcaatcc tgaaccagta ttatcagctc gactcataac aacaagttct
     5581 gttgttaatg gatctaaatt tctttctata cattcgacac ttgcgtaaaa gcttttcata
     5641 tcgattaaaa agtaatcatt tactggctct tttgaataat ccatcataaa taacactcca
     5701 ttctttcaca ttatacaaac atatgttcga tattgtaaag aagaaatagc taaatacata
     5761 aaaaaagaag cataggtatg ctatcaccct gcttcttctt ttgatttgta tgatcaattt
     5821 attctatcac tttactaatt aatttgaaaa tctaataaaa actctcactc taaaagagtg
     5881 agagactgca aattagagta cggacttttt tggagttccg ttctttaata ttaacaaatg
     5941 aataaaatcc ttacaacatt tgagaataag ttatcagtag acacttacaa aaaattctca
     6001 tactatttcc tccatcatta gtttttgtct atataaggtt taccctaaat agacactggt
     6061 ataacattga aaaatatggc ttcctcgtgt atgattaggg tgtattttaa atagttgcag
     6121 aaagtaggta aaagttttga gagttgcata catacgtgtc tcatcgatcg atcaaaatga
     6181 acaacgccag attgaagaaa tgaaaaaatt cggtgcagag agaattttca tcgagaaaca
     6241 atctggcgca acgattactc atcgacctgt tttccaagaa gcattagatt acgtccgaga
     6301 tcaagatatt tttattgtgg aagctattga tcgacttgga agaaattatg acgagatcat
     6361 cgcttcagtc aattacttga agaagaaaaa tgtgaagctg atcattacta gtttaccgat
     6421 catggctgaa gcaattggca atcctctcct ggataaattc attaaggatc tgatcatcca
     6481 aattttggcc atgatcgctg aacaagaacg gactgaatcg aaacgtcgcc aggcacaagg
     6541 aattaagatc gcaaaagcaa acggcgtcta caaagggcgg cccaaattat acagtgcaga
     6601 cgcaaaagat ccccaacgtc gccttgttta caaaagcatc gttgaagatc ttaaaaatgg
     6661 tgttgcgatt gctaagattg ctaaagacta caatgttacg agacaaaccg tgtatagaat
     6721 aaaaaaagac agcatggtca atgacaaata gttgatcatg ctgcttttat tatcttctca
     6781 tctttaattt cctacatata attactatct ataaaattaa gtcttactca gaacagataa
     6841 agcataaaat tccaacaaac cattttttac ttaactaaat aaagagtctg ttaatataat
     6901 cttcacttat ttacgttaat cccctactgt atttgttggt tccttatcta tattttctac
     6961 aaaatcctta ttatcttgtg atttagcttc gtataattct ttgtatgtcg acacattggt
     7021 tatactaatt cctatagcta gttcttgaga ctctactttt ctctctagct cctttgacaa
     7081 agtgatttga gatttatttg gttccgttgg agtcaattct atagattctt cataaaaatc
     7141 tctatacata aagctatccc tgaataattc atagctttaa ttctttggca cattttattg
     7201 aaatatgtat cactaatatc cctatcaact gattttgact aaaaagttac ttcaaacatt
     7261 cactgttcaa aaatattgag gaataaattt tgggtgtaag gcattttgag catacttctc
     7321 cctggataac ttcccagtaa aaaaatcgtt agattgttgg attagatcca ttgactggct
     7381 cgcctcaggc gagcaaagac cctgtagaat tcattctgat acacatgata actagataat
     7441 ttgagataga ctcgtctacc cgtttggact aatttcccag caactttcag aaacttcaat
     7501 cgaatagaat gaatggtcat tcccttttgg acttcttcaa agccaatcgt tcttaagaag
     7561 ttgactaagt tgtaagctat caagctgagc atcattcgga catgattctc caaaaagcga
     7621 ggactgtctg ttttgtcaaa gtaaaagcca gctttcgctt ctttaatgaa gttctccatt
     7681 gtgccacgtt tggcatagat agaaaagatg gtatcaggag aaacattttc tgatagattc
     7741 gtcacgataa attcatgtcg aaagagtagt tcgcccgctt cacgtgttga gcgtatacac
     7801 acgcgacgac tttgtgacca ggattgtgct tgataagtgg tcgagaagta ctgaacttct
     7861 cgttcttccc acttttgatt atcgccataa agtactgatt tctcagctat ttgacctagt
     7921 ctacgattat tcttcagtcg aataacataa tgactctttt ttgattcaca cgaatcatac
     7981 acaccaggtg tagcgaaccc gctgtctcca cgaaccaaga tgtcagtgtt tggtagagag
     8041 tgattatagt gctctaataa aggtgtgaga aactccttta cgccttttga tgtatattga
     8101 tttcctgaac gtagttcagc ttttaagaag tcaccagtca atccgtcaaa agctaccaat
     8161 ggatggtaac cataggtttg gtagtgtgta ttataattcg tttgttcttg gtgaccaaat
     8221 gtatctgaat gggtcgaatc taaatcaatg attaattccg tatcattacg gatgaggcgt
     8281 gctttatcga taagttcttg gttcaaagct tgaagttcat ggatattctc ctcagatagt
     8341 cgatctaaaa atcgggaaag tgaagattga gaagcgagtt ctttcctatc taaaacagct
     8401 ttaaacacag gatcttgtct caggagatta gctgcagaat cagctgagta accagcaatt
     8461 aattgcataa tgaactgctc aagtatggat aagttgtcat gagtaaaata cgctcgttcg
     8521 tcttcaatgt gaatgtgttg cttagccagt tcagaaaaac caaagatgtt catcagctct
     8581 ttcactagga cgagacccga atcactcgat aattgaccac ctgtatgaga aatagtgata
     8641 tttgaattga attttacttg gtttttgtgt aagctagtca tgaaaagaac tcctttcttt
     8701 tggttggttt agtcacttta accatagcag aaaggggttc ttttttcatc acttaacggg
     8761 tgaagatgaa aaagtaattg taagctgccg tgaggcagtt tcacatggtt ttaattaaaa
     8821 gtatgaatta ttcaggctat cattaatttt ataaaaaaaa ttaaatgttt ttagcactgc
     8881 catataacat tcaaaacatt cttctatagt tggtttactt ccttgaggag aatgaaccaa
     8941 tttattacgt ttttctatga aatttttaat ttcctttctt atttctttcg gaaactcaat
     9001 attaaagtac cctttaaaca tgctccctaa taatttgtct acagggggag attgtaaatt
     9061 ctcaaatatt agagttaaat caggtttctc ttgttgataa aattccttta cccctatttc
     9121 acaggctgta attatcgaaa taaacctatc tctagtattt gatatatttt tacttctttt
     9181 aaggagtata agagaaaagg ggatactttc gtgtccatgc atgtaattaa ctaattgttg
     9241 agcttcagaa gttatatctc taattatata gttaacggaa ttaggaagtg tctttaactc
     9301 taatgaaatt tctatccaat tactatcttc taatctgtat atgctcgaag tataatttga
     9361 ttcgtcagca agcatcgcta catcccaaac actgatcata aaatctttta aacgttcaat
     9421 catttcagca atattgctaa aatcagctaa tttttcttta gaataaaaaa aaacatttat
     9481 atcagaatca ataaattcta tttttttatt actatatcgt catcattatc ttgaccattt
     9541 atgtttattg ttccctgagt ggggagtact tctttcaaat tcaagttaaa tttatattcc
     9601 attacttctc ctccaatata taaaaattac ttaactgtat ccaaatttaa tatgtaaata
     9661 aatcattaat ttttagctaa attctatgag aagtttattt ttgttgtttt tgctaattaa
     9721 ctaaaactag tacaaaaaat ttaaaataga ttattaaacc tattgcttat tctattcagt
     9781 catatatata agcgtcatcc tttagtaact aacatcccac actacaatag tattataaca
     9841 tatctattca acttcaaatt gttgaaattt aagccattta tatttacttt tcctcaactt
     9901 tagaaatgat tttactaacc ttatatccgt aatacgcgta acaaacacat tcattaataa
     9961 caatcaacac taagctatat tagcttttta aatgactagg gtaaataaaa taaggatcaa
    10021 aaacttttaa gtttctgacc ctttccttac ttccgattgg ttaatagtga tcttttgcag
    10081 aataaataaa tatcgtttca ttttcaactc tatatatcag tctatgttca tctgtaattc
    10141 ttctggacca ttttccagat aaatcatgct ttaatggctc aggttttcct aatccagcaa
    10201 agggggaacg atcgatatct tttattaact tgttaatctt ttttatattg cttttgtttc
    10261 cttgctcatg ccaataaaga taatcatccc aagcatcatc agaccaagcc ttaatcatca
    10321 gattcaacct cgattaagtc atgtgtttta aatgcacctt tggagaattg ttcatctcct
    10381 cgacgaattt tttccatgac gtaattatta gaaagtgttc tcaacgtttc ttgcatagaa
    10441 tcataatctc tttttgataa tacaacaact gtatcttcta catctttact tgttacaata
    10501 agtgtttcag catcctcatt aacttgtttc atataactac gtaaattttg gcggaaattt
    10561 gaataagcta ctgcttccat tcctttcacc ccttatttat ttctataaaa caattgtaca
    10621 ttatattgta caattaagca aatgattaat attctaactt cttataatta aaagtgctat
    10681 cacttttagt ttttaggata aaaagtgacg tcactttttg agtgctggta cctcaaatcc
    10741 tgctttttct tgggcaatca ataaatctaa cgcaatacac ataatcctgg attcactttt
    10801 gccgtacttc tgctttagtt gctgcaattc tttttgtctc ttttcatcca acgtaacggg
    10861 taaccgttta tttcctattg caattggcat tagtactctc tcctttcatc gaaataaccc
    10921 tatgaaaaat atggctgcaa tcatcttgaa tagtagactc acaagggcta atacaaagcg
    10981 ccaaaagatc aaacaaaata ccagtattgc caaaaatact acgaagttta aatgcacagc
    11041 tgcagcatgg cgcataagta tttcgccaac aaataggact aacaatagag gaatgatttt
    11101 attcatatgg atctccttct ttctcttagg ttatcgggtg ttgcccgctt atttcgcacc
    11161 acctaagcgg cgggggcggt tggagcgatt ggtgcgaaat aagaaccatt cccttagtta
    11221 ctaggttttt tgtccttatt tcacacattc tcacttttcg gtataaatga ttattgcctt
    11281 tttttccttt caaatcgttg atatggcatt cgtttacggt caagttcatt ttggatttct
    11341 tctgtatcgt cgtaagtata ggtgtctaaa aaatcagtta aagtcgtgta ccagtcgtcg
    11401 cctgggttat ctacttcttc tacatattcc ctcaaaaatg cttcgggtac agcgagttca
    11461 taatccattc ccattaacac gatagattcc gttccacacg tcaaaaatct agtaaataca
    11521 aggtaataat cgtctatcgt cgcatctttg ctgtttagct cttccatttc ttcttccatt
    11581 aattccatgc tctcaaaaat tgaataatat tctgattctg agtaatagtg tttcctgcaa
    11641 atcgtgtagc tgacaaagtt ttcgaccagc tcactccttt ctaggtaaac attcatgatg
    11701 tatttttctg tttctagtcg tagaagctcc gttccttttt ttaccgtttc aggacgacgt
    11761 ttatacacgt ctaaacgatt atggctgact gtgaccttcg tttgactcgt ttcactttcc
    11821 aaatacaatt gttcttc
//
LOCUS       DAXWLB010000082        11676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXWLB010000082 DAXWLB010000000
VERSION     DAXWLB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.082"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            127..1386
                     /gene="lysA"
                     /locus_tag="ACGE4H_001710"
     CDS             127..1386
                     /gene="lysA"
                     /locus_tag="ACGE4H_001710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFF8365118.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1373..2173
                     /gene="dapF"
                     /locus_tag="ACGE4H_001711"
     CDS             1373..2173
                     /gene="dapF"
                     /locus_tag="ACGE4H_001711"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFF8365119.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2176..3057
                     /gene="dapA"
                     /locus_tag="ACGE4H_001712"
     CDS             2176..3057
                     /gene="dapA"
                     /locus_tag="ACGE4H_001712"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFF8365120.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3054..3770
                     /gene="dapB"
                     /locus_tag="ACGE4H_001713"
     CDS             3054..3770
                     /gene="dapB"
                     /locus_tag="ACGE4H_001713"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFF8365121.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3776..4471
                     /gene="dapD"
                     /locus_tag="ACGE4H_001714"
     CDS             3776..4471
                     /gene="dapD"
                     /locus_tag="ACGE4H_001714"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFF8365122.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4487..5488
                     /locus_tag="ACGE4H_001715"
     CDS             4487..5488
                     /locus_tag="ACGE4H_001715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFF8365123.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5485..6846
                     /locus_tag="ACGE4H_001716"
     CDS             5485..6846
                     /locus_tag="ACGE4H_001716"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFF8365124.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6806..7957
                     /locus_tag="ACGE4H_001717"
     CDS             6806..7957
                     /locus_tag="ACGE4H_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFF8365125.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7932..9101
                     /locus_tag="ACGE4H_001718"
     CDS             7932..9101
                     /locus_tag="ACGE4H_001718"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8365126.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9276..10655
                     /locus_tag="ACGE4H_001719"
     CDS             9276..10655
                     /locus_tag="ACGE4H_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8365127.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10936..11676
                     /locus_tag="ACGE4H_001720"
                     /pseudo
     CDS             10936..11676
                     /locus_tag="ACGE4H_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         3899 a         2038 c         2596 g         3143 t
ORIGIN      
        1 aaaaatattt cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa
       61 aattcaaatc tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat
      121 gaaaaaatga cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat
      181 aagacacctc tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag
      241 cgaggctttg tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa
      301 acgattgaga tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt
      361 ggggaaatct ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt
      421 aacaacaaga cgccagagga actgatctat gcacttgaaa atgatttgtt acatattgtt
      481 gccgataatt tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa
      541 atttgtatca tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc
      601 actgcgcata ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc
      661 cttcagttga taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg
      721 tcacaaattt ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta
      781 accgattttg accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca
      841 caaaatgatc agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa
      901 gaagctttgg aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt
      961 attgttggag aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat
     1021 aaaaaatatt actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag
     1081 gcagaatacg attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg
     1141 attgcaggaa aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa
     1201 gcagaatcgg gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc
     1261 agcaactaca atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga
     1321 ctgattgttc gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct
     1381 tcataaatat aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga
     1441 ttatagtcaa ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat
     1501 cgccgtaaaa atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc
     1561 gatgtgcgga aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa
     1621 tgaacaggaa ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc
     1681 ctcacctttt tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa
     1741 agctaatcaa acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac
     1801 gatcactagt ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga
     1861 ggaagtctgg agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac
     1921 caatgtcaac tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag
     1981 aggagtaggt tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa
     2041 ggaaactggg aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat
     2101 gatctctggt gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat
     2161 attggaggaa tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa
     2221 gagggatcag tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa
     2281 acagatggat tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag
     2341 gaggagcaga tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca
     2401 ggagctggaa cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg
     2461 ggggcagacg cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta
     2521 gctgctcatt ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt
     2581 ccttctagaa caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct
     2641 aatatcattg gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg
     2701 ttgattggag aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg
     2761 agtgtaggag gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat
     2821 gaattagtaa aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat
     2881 gcacttgaac tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc
     2941 atgtcattat tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa
     3001 gagaaaaaga aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa
     3061 tcggactgat tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag
     3121 gagatacggt ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg
     3181 aactgccaga actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt
     3241 atagcttatc atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt
     3301 tccaagaaat aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt
     3361 tcggcatcat ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc
     3421 agatcgaact gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa
     3481 atatgctgct tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag
     3541 ggaaaaaaag agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg
     3601 gtgaacatga tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt
     3661 tttctaatcg aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt
     3721 caccaggtta ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga
     3781 agctcaagaa atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta
     3841 tctgaatgta accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag
     3901 cttcgttgtt tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa
     3961 aatcattgaa atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat
     4021 taaaaaaatt aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg
     4081 caacaatgcc gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa
     4141 cacgatgatt gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca
     4201 tattggtgca ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat
     4261 cgtagaagac ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg
     4321 aaaagatgca gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt
     4381 tgtcggtgga atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac
     4441 agcattgatt gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga
     4501 tacctgtatt gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga
     4561 atatgatttt ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt
     4621 ttgtgtattc aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg
     4681 atatgattta gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc
     4741 acgaagtcag ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat
     4801 tccattaatc gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa
     4861 tccaaattgt tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg
     4921 tatccagcga gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat
     4981 tgaagatctt catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag
     5041 ccaaacggtg attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca
     5101 gaaaatgata aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac
     5161 ctgcgtacgt gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa
     5221 agagtttacg acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga
     5281 agatgatcta gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt
     5341 ttatgttggg aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac
     5401 tgccgataac atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat
     5461 gcagaaaata gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga
     5521 acatcgatgt ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc
     5581 aatcgccaag tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta
     5641 acagacttac tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt
     5701 tggaaaagga tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct
     5761 attgaagatg aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat
     5821 tatttagtaa gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac
     5881 cagtttattg acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa
     5941 aaaacagcac aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg
     6001 ttttacgggg cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt
     6061 accggcgcga tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt
     6121 tcaggaatca tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc
     6181 tcttttagag aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct
     6241 gttttcccag ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc
     6301 gctcaaggga caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata
     6361 gctggacgca aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc
     6421 gggtttatct ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt
     6481 ccttcaggga tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta
     6541 tttctcatca cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa
     6601 gacttagctt tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg
     6661 aaagtgttat cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca
     6721 caggagttga atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt
     6781 atctatgaag ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat
     6841 tcctgaaatc gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa
     6901 cagcgaggcc gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca
     6961 gcaagaagaa acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac
     7021 gaatctctct tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca
     7081 tatggcgatg cttttagggt tgactgactt tataactaaa cacattgagg atcttattta
     7141 taatgtagta ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat
     7201 tcagtcagga ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc
     7261 tggactagct aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga
     7321 aacagacatt atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga
     7381 tagtctggag gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc
     7441 ggcagagtgg aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt
     7501 tttagctgga gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa
     7561 ttatttgaag aggggattag accatttagc agaacagttt cactctacag ctactatttc
     7621 ttttcgctat aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact
     7681 gttagattca aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg
     7741 catatatacg caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc
     7801 tttacatgat ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta
     7861 caaaacgatt ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga
     7921 gcgagtaaat aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag
     7981 tatcaaaaat acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca
     8041 ccctgggaga accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga
     8101 ttgaagaaga cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg
     8161 cttgtatatt tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga
     8221 cgacagttgg tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag
     8281 acaaggttct tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg
     8341 gtgctgaatt gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat
     8401 ttgaacgagc atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta
     8461 acaatcctac tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa
     8521 aaaaatatcc ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa
     8581 cgcatatgtc catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca
     8641 agtcacatgc gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg
     8701 acgaactaat taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag
     8761 ctgcggctga agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc
     8821 aaacacgaag agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc
     8881 caaacggtgc attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc
     8941 agttttgtgt agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg
     9001 gcccagaagg agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg
     9061 aggcctgcaa acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa
     9121 aaacaatcgt taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt
     9181 caaatgaacg cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga
     9241 atgattttaa aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg
     9301 taggtgcatc tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa
     9361 atgtagatat cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg
     9421 agctgtattt agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa
     9481 actttccgca atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg
     9541 ataaaaaaac agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg
     9601 acaaattgat tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc
     9661 tagaaaatgt ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa
     9721 atgatccggc tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag
     9781 cagaagcaag ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt
     9841 taggcactta tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg
     9901 gtattgaagt tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg
     9961 caatcaaaac aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga
    10021 aggcaaatac agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa
    10081 caaatgaata cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg
    10141 cttattctat cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag
    10201 aagcacgtta tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag
    10261 tcgtaggttc ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata
    10321 gctttacagc agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac
    10381 gaccaaaata tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg
    10441 atccaatgac ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc
    10501 aaggaaatgt attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag
    10561 ctgacttctt cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc
    10621 aacaagcgtt aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt
    10681 tgtgaaaaag ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    10741 tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca
    10801 aaaaagggac tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa
    10861 cttttggagg tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt
    10921 tcatgattat ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa
    10981 gcggaaatgc ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat
    11041 gaactaagaa accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag
    11101 ctaggaataa aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga
    11161 aaagttggag cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat
    11221 cagaaaatca cgacggatac aactgaattt aaataccatg aagtggatgc caaaggcatt
    11281 atccgtcaaa agaaattgta tctagatcct tttatggata tgttcaatag tgagatttta
    11341 tcctatcgta tctctgaaaa gcccaatgca ttagcggtaa tggaaggttt ggaggaagct
    11401 atccagacca ctaacgattg tccctatcgt cgtacgtttc actcagatca aggttgggcg
    11461 tatcaaatga aagcttatag aaacaaattg aaggagtaca aaatctttca aagcatgtct
    11521 cggaaaggga actgcttaga taattcactt atggagaatt tttttggctt attaaaacaa
    11581 gagatttttc acgggaaagt ttataactgt ttcgaggaat taaaatcagc catcgatagc
    11641 tatatctatt actacaataa tgaacggata aaacag
//
LOCUS       DAXWLB010000083        11616 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXWLB010000083 DAXWLB010000000
VERSION     DAXWLB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.083"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(397..1053)
                     /locus_tag="ACGE4H_001721"
     CDS             complement(397..1053)
                     /locus_tag="ACGE4H_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8365128.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1246..1524
                     /locus_tag="ACGE4H_001722"
     CDS             1246..1524
                     /locus_tag="ACGE4H_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365129.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1543..2751
                     /locus_tag="ACGE4H_001723"
     CDS             1543..2751
                     /locus_tag="ACGE4H_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365130.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLPARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2899..3264)
                     /locus_tag="ACGE4H_001724"
     CDS             complement(2899..3264)
                     /locus_tag="ACGE4H_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFF8365131.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3411..4184)
                     /locus_tag="ACGE4H_001725"
     CDS             complement(3411..4184)
                     /locus_tag="ACGE4H_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8365132.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4358..5218)
                     /locus_tag="ACGE4H_001726"
     CDS             complement(4358..5218)
                     /locus_tag="ACGE4H_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFF8365133.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5218..6636)
                     /locus_tag="ACGE4H_001727"
     CDS             complement(5218..6636)
                     /locus_tag="ACGE4H_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8365134.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6693..7142)
                     /locus_tag="ACGE4H_001728"
     CDS             complement(6693..7142)
                     /locus_tag="ACGE4H_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365135.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7587..7850)
                     /locus_tag="ACGE4H_001729"
     CDS             complement(7587..7850)
                     /locus_tag="ACGE4H_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFF8365136.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE4H_001730"
     CDS             complement(7949..9244)
                     /gene="rho"
                     /locus_tag="ACGE4H_001730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFF8365137.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9241..10512)
                     /locus_tag="ACGE4H_001731"
     CDS             complement(9241..10512)
                     /locus_tag="ACGE4H_001731"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFF8365138.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10677..11546)
                     /locus_tag="ACGE4H_001732"
     CDS             complement(10677..11546)
                     /locus_tag="ACGE4H_001732"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFF8365139.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
BASE COUNT         3255 a         2461 c         2016 g         3884 t
ORIGIN      
        1 cgtgtcacag ccccttttgt tgcaaataaa cggcgttcaa aagcttacat ctacggtgat
       61 aagtccagcc aagttaaaaa cataagagac tgtttttact tggctcttct tattacttga
      121 tgcagaacag ctttttcaca acctcttggt aaacggtgtt ctcctaccaa ctcctcggtc
      181 acaagtcaca atattcatca aacatgaaaa gctgtttttt gaaattgttc cttgtgactc
      241 agagttaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
      301 tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt ctctaatact
      361 caggtcaaaa cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa
      421 caagtccata ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt
      481 cagttgtttt aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat
      541 ttcttcattc gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa
      601 atcatataat tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg
      661 ctgcattttt ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg
      721 tagctttggg tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc
      781 tgcatattct gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc
      841 ttttaaaaga gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc
      901 atttgaaata acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc
      961 catcccttcg aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt
     1021 atccaaatat tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc
     1081 taaaaagatc atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata
     1141 ttaacatttg ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc
     1201 gttatactgc agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg
     1261 aacacaatca ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct
     1321 ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag
     1381 aatccaacaa acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt
     1441 attcgccaac ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa
     1501 aaattaaaag catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt
     1561 aataaatatt ttattggaat cacattgatc aattttattg tctacctgat ttattatctg
     1621 ttgatggtca tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca
     1681 ggttttgctt ctgggatcta tatcatcgga acattgccgg cacgcttatt catggggaaa
     1741 cagttagaac tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt
     1801 tcaacattag catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg
     1861 aatggattcg gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc
     1921 cctgcatcta aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc
     1981 gctgttgggc catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc
     2041 tggttttcaa ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa
     2101 aatattccat tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt
     2161 attgaaaaga aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca
     2221 agtgttttat catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca
     2281 tttttcttcg tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt
     2341 ttcgatgtca aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga
     2401 ttatttttac tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc
     2461 ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc
     2521 gaacatcgaa tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc
     2581 gttggtcctt atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg
     2641 attgccggag tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat
     2701 agtcatgaag cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga
     2761 atagataaaa aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata
     2821 gtttatgatt attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag
     2881 aatagtttaa ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag
     2941 caaccacttg tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt
     3001 catccaaata tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc
     3061 tgaaccagca tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc
     3121 ccctagcaca tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct
     3181 aacaccgtat aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac
     3241 taccaacgcc ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga
     3301 ttatctaaca agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat
     3361 aaggtcctat ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac
     3421 agacattcga tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt
     3481 gtcaccagat aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt
     3541 ttttgctgtg taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc
     3601 attgcctctc cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc
     3661 attatcgcta aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt
     3721 gttgctcgtt tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca
     3781 ctcaacatag cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct
     3841 aggtttctga ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct
     3901 cgaatcaaag aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg
     3961 gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg
     4021 cgaactctac ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct
     4081 tcgctataat ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga
     4141 taattcacaa tctcctgctg tctattctcc acttcattca acataaaatc accccattta
     4201 ctttatatag taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag
     4261 aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag
     4321 actattgtca taccctcttg accacacatt tttattttta gtataaacta gccttgccta
     4381 ctgtgttcaa aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg
     4441 gataaattgc attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat
     4501 tgaaaaagac gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg
     4561 cttcgctgac ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga
     4621 tttgttggtt caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat
     4681 acaagccatg cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa
     4741 aaatcttcgc ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac
     4801 cattattccc aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg
     4861 caaccacttt tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca
     4921 tcacggaaga atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat
     4981 ccaagtggat aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa
     5041 actcatcact taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta
     5101 atctctctgc ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg
     5161 gtactgcaaa ttgattctct ttagccactt tcaataactc tttcattgta tataacatta
     5221 cgcttccacc tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt
     5281 cactgttttt ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc
     5341 caagacatac ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc
     5401 atgaggtgcc attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc
     5461 tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc
     5521 tgtaatatac gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt
     5581 aaatttatgt ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc
     5641 agccatacaa gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt
     5701 cgaagtcgat ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa
     5761 tccgaacaat agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa
     5821 cacccatgtg atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga
     5881 taaaaagggg acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt
     5941 atttgccaga aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa
     6001 acctgaacca cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt
     6061 tccagcaatc gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt
     6121 atctcctccg atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc
     6181 agccgctgta atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa
     6241 tggaagcatg taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt
     6301 tgcagctggt tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc
     6361 tattactact tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc
     6421 ggcaaatcgg tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt
     6481 gatttcttgt tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg
     6541 atggtttaga tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat
     6601 tccagtcggg caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca
     6661 ctatcgatac tcggacaaag actctactat tttcattctt gactaatctg tctgatgata
     6721 tctgctggcg tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg
     6781 gagagtgtgc tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca
     6841 aaaatatatt caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga
     6901 aaaaaacaca gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca
     6961 tcttctattc ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga
     7021 acatttctta ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct
     7081 tttgaatcta tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc
     7141 ataaaaaacc tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag
     7201 catcgctagc tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc
     7261 gtgtataagt cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa
     7321 caaaaagcct tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact
     7381 tcgaaataac ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa
     7441 actatatact agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa
     7501 acgaagtttt aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc
     7561 taaacgttga gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat
     7621 tttttgttga aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag
     7681 aatggatgtg agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc
     7741 cattcaactg tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt
     7801 gaatccatga atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg
     7861 ctcctttgcc ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca
     7921 tcttaacatg tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt
     7981 taccaaagga gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca
     8041 aaaagcggat cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt
     8101 tccaaatttc ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt
     8161 ttttgatatc gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt
     8221 ctagatttcc agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat
     8281 cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc
     8341 cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc
     8401 cgcttggtgg aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga
     8461 taacgacatc tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa
     8521 ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc
     8581 ttcgttctag atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta
     8641 cttccggata gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg
     8701 cttttggcgg tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga
     8761 tcattcgggt agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa
     8821 gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa
     8881 cgctttccac gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc
     8941 ctgccacttt gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga
     9001 tatcttctgc acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa
     9061 cgatatccaa tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca
     9121 cagctagaga taattccttt ttattcatct tactgtaata aggaatttta aattctttgg
     9181 cataactata aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc
     9241 tcattcagct cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc
     9301 gataatatga tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc
     9361 tgccaatagt ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc
     9421 tgtgccgtga agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc
     9481 agcgcccatt cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat
     9541 gatttctgaa gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt
     9601 ggcaaatcct ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta
     9661 aacttcgatt tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat
     9721 aaagctttcc aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc
     9781 tgctaatgct agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc
     9841 atgtaatgtt tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc
     9901 ccccatatta ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc
     9961 aattactgtc tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac
    10021 tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata
    10081 catggcacca tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag
    10141 gtcaatcggt ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa
    10201 tttaccaagc agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg
    10261 cggcatcgga atcgatacga cttctcgagg atcgatttct agtatgttgt tggaaaaatg
    10321 gacagttgcg cccatgatct ctaaaatttc aattaatgaa tgaacatctt gtatgtccgg
    10381 cacaccttcc aagatcactg gggaatcagc taagattgca gctggaatca atgcgactgc
    10441 actatttttt gcaccgctga tcgttacttc accagctaaa ggtcggtttc ctttgattac
    10501 gatttttttc atctgtttcc accttgttta ttaatctttt ttcatggata tgcttgtatt
    10561 ttatcataga taaaaaaaag aaacgagcat ggtctgccgt tttaagtaaa aaagaatgga
    10621 gagcgacttc aaaagtcgtt ctccattcct atatatcgta tctgttatgt ggatgcttac
    10681 gctttgttag ctgaaccgaa ccagtcgata tgagatttag catcttcaac gattgctgct
    10741 ttacctggag ctaataattt acgagggtcg aatcctttac cttctttatc tttaccagct
    10801 tcgatatatt tacgagtagc tgcagcaaat gataattgga attcagtgtt aacatttact
    10861 tttgagatac ccattgagat agctttttcg atttgatctt gaggaatacc tgatccgcca
    10921 tgtaatacta aaggcatgtc tggacctaca gcatcagcaa ttgcttgtaa gtggtcaaaa
    10981 gctaatcctt tccagttttc tggatattga ccatggatgt taccgatacc gcaagctaag
    11041 tagtcgatac ctgtagcaac catttgtttg cattcttcaa tatcagctaa ttcgccgtta
    11101 ccgatgattc cgtcttcttc tccgccgatt gaaccaactt cacattcaac tgagatacct
    11161 tttgcatgtg ctttttcaac aacgtcttta gctaatttca agttttcttc aaatggtaga
    11221 tgtgaaccat cgaacattac tgaagtatag cctaattcga tacattccaa agcagcttcg
    11281 taatcaccat ggtctaagtg gatagcaact ggaacagtga tgttcattgc ttcaactaaa
    11341 tcagtgatta aatctttaca gattttgtaa ccacccatgt atttagctgc acccattgat
    11401 gtttggataa gtactggtgc ttgttttgct tcagccgctt caaggattgc ttgagtccat
    11461 tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc ctttacgagc agcttttaaa
    11521 aattcagctc ctgatactac tggcataata aaattcctcc taagttttgt aaaactaaat
    11581 ttgttaagac aattcttaac caacgaccct atttta
//
LOCUS       DAXWLB010000084        11609 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXWLB010000084 DAXWLB010000000
VERSION     DAXWLB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.084"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(44..841)
                     /locus_tag="ACGE4H_001733"
     CDS             complement(44..841)
                     /locus_tag="ACGE4H_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFF8365140.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(838..2022)
                     /locus_tag="ACGE4H_001734"
     CDS             complement(838..2022)
                     /locus_tag="ACGE4H_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFF8365141.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2047..3294)
                     /locus_tag="ACGE4H_001735"
     CDS             complement(2047..3294)
                     /locus_tag="ACGE4H_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFF8365142.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4H_001736"
     CDS             3509..5254
                     /gene="dnaX"
                     /locus_tag="ACGE4H_001736"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFF8365143.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5274..5588
                     /locus_tag="ACGE4H_001737"
     CDS             5274..5588
                     /locus_tag="ACGE4H_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFF8365144.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4H_001738"
     CDS             5613..6209
                     /gene="recR"
                     /locus_tag="ACGE4H_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFF8365145.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4H_001739"
     CDS             6273..6917
                     /gene="tmk"
                     /locus_tag="ACGE4H_001739"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFF8365146.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            6932..7261
                     /locus_tag="ACGE4H_001740"
     CDS             6932..7261
                     /locus_tag="ACGE4H_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFF8365147.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4H_001741"
     CDS             7275..8213
                     /gene="holB"
                     /locus_tag="ACGE4H_001741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFF8365148.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8249..9073
                     /locus_tag="ACGE4H_001742"
     CDS             8249..9073
                     /locus_tag="ACGE4H_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFF8365149.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9066..9413
                     /locus_tag="ACGE4H_001743"
     CDS             9066..9413
                     /locus_tag="ACGE4H_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFF8365150.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4H_001744"
     CDS             9482..10354
                     /gene="rsmI"
                     /locus_tag="ACGE4H_001744"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFF8365151.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4H_001745"
     CDS             10463..11584
                     /gene="dinB"
                     /locus_tag="ACGE4H_001745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFF8365152.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
BASE COUNT         3714 a         2174 c         2512 g         3209 t
ORIGIN      
        1 ttttgtcaca gccctttttt tgcgaacatt catttagcaa tttttagaaa cttctgccgc
       61 caccgccaaa agtcccgcca ccgctagaat gggtagtact tccgccaccg ccgcttccag
      121 gaggtgggct tttggggatt cttcttgtcg tgacaaaaga atttgtcaga cgatcggttt
      181 tttcagttaa tttgatactg gatttttctc ggaatggata tttgtagatg cctgatttca
      241 attgataacg cgaaacagtc acaaaataaa agacaagact aaggataaga gccaaaacaa
      301 cagcaatgat gatttctact gtcgtcaaaa ccttatagcg tgtaattttg ccagtttctt
      361 cgtctacacg ataatgtcct cctggaactc cgtcttccac atatgttgct gatttagaca
      421 ggtaagcttt tgcagcattg taataggcag catttgtcat ctgatcataa acatcatcta
      481 agatcgaatc gattctgcta tcagtcaaat aatcgatcat attaccagaa gtcgaaatgt
      541 agatttcacg ctgtcccata tctaatagca atactgagcc attttgatca tttccgactg
      601 cgtctcttaa gtaattatca gcaaagtctc ttggttcttc tgaattagat gtagtcgtta
      661 cgatcaagac tcgtcctttg attttttcat tcagcttttc tgcctgttgt tccaaagatt
      721 ggatttgatc tgttgtgaat aacccagcct catcatttac cgtagctgta tctgcatgag
      781 tagccaccgg ccagccaaca cttactagca aaagaaagaa taaaaaaaat tttttcatca
      841 tatcaagtaa cctccgatca gaaagaggat cgctagccca gcgaaaatcc caagagcggt
      901 cagtcccaat tttttctggc tgatcggcag gacaccgctg acctttcctg tttgtccatt
      961 cattgcgtag tagtagactt ttttacttga gtcattgctt cgataagtaa caagccatac
     1021 cggcagtaat acgtaattat ttacttcgtt tgtgattgaa gcagaagttc gcacaccggt
     1081 caatgttgta tatccatttg cggtatcttt taacagcttc tctgaatagt cttttaattc
     1141 agattgaatc tgtgatttga ttgcctcata ttcgatatct cttttttctg cttggaaccc
     1201 ggctaaatac tggcttttaa aatcaattgc tttatctaac gggaaaggtt gcacgccttc
     1261 gaccatcttt tgttgcgtat tttttgataa ggcatttttt actagttctt taaaagaaag
     1321 cttgcctgct cttgaaacag caaattgctt cgtttctgtg tattcgatgt cacctatccg
     1381 ccatatgcgg ataactgtac cattcgcctg catggcacca tctacttcag catctgtagc
     1441 ccaataaggg aaatacacac ctgtcaactt atcgatttga tccttattga aaaaagcttt
     1501 gggaacaaac cattttttct tggtccaagc gagaaatctg ttgattgctt cttctttctc
     1561 gacagcaaaa ggaagtactt tatttggcaa gaattctccg cttaaacgtc ccgatagtac
     1621 gacgggatta tggcagtagt aacaataagt cgcagcagtt gtcgcatcag tcacgatctc
     1681 tgcaccacag ttgggacaaa gaaaaagatc tatcgctgcg gtattatctg cagattcgtc
     1741 cgctgtatct gaaaaaccgc ctgcctcgtc aaccgctttg cgctcggctt cattcatatc
     1801 ggcaagctgt tcttgggctg taaaagtaaa atcttcggta ggtgaaggtt cttctttgct
     1861 ttcttcgcca gccatacgcg cttctttttg cttttgctca tattggctga cttcttcttc
     1921 tgtatagata ttcaggcagt attcgcaatg aaatttttgg tctttgggat caaaagtcaa
     1981 cggaccgccg caattaggac atttatgtgt aagaacatcc atcagctgca cacctttcta
     2041 aataaattaa ttaacgggga ttcctttaaa cggtttacca cagtttggac agaatttagg
     2101 gatgccgttg gaaagatcca cgatctcatt gcattcactg catcgcattt gtagcttagg
     2161 ctgaacgttt tctacaggtt tggcagctcc gcagttggaa cagaattttc cggaattttc
     2221 tgtcccgcaa actggacatg tccaagtatc cgcatttcct tgtgcagttt gttgattttg
     2281 tttttctgct tgttgtttga tttgttcttg gttattttga gctgcttgag aaagataaga
     2341 accatttgca ttcattccca tacctacacc cataaagccg gtcattgctc ctgcttcatt
     2401 ttttccggct gcttccatac cacgtgcaat ggaaccttgc acgtatcctt cacgtacgct
     2461 aggatcacct agcatcgccc cttcgttacg catattgatc agtttcacag aatcatcggt
     2521 gtaagaaatg ctggctacag ccacagaaac aatctccatc ccacgtaatt cacgccaaga
     2581 atcatccaat gcagtatcca tgtatttgct taattccaaa ctcttagatg gtacataaga
     2641 aatccgttga ccatctgcag acatctgatt gattgctgat tgtagcgctg ttaggaattc
     2701 agctaaatac tgctcattga tatcattgat ttccacttgt gttttgtttt tagggattgc
     2761 gtttgtataa aataaaatcg ggtcaacgac atgaatagaa taagtgccat gtgcccgtaa
     2821 gaataattct gcgttataga aattgtcaaa gtagttcaac ggcgcgcttg taccgaattt
     2881 gattcccttt atttcttgga gattgatata aaaaacttgt tgtttctgcg gtgtgacgcc
     2941 gccaaatttg aatcggtcaa aagtttcagc aattgcttct ttcagtgttc cattgaacat
     3001 ggaaggagct gcgtcatttt taatggtgta ataaccttcc tcagcggtat aatcgatgat
     3061 ttttccacca tcgaccaaaa gcatcatcat gtttggatag acatggacga ctgttccgtc
     3121 tgttaaaaca tcttctgttc ctttacgatt ggagccacgt ttgtcatctt tgcggacttt
     3181 gacacctttt gtcatgaccg ttgtatcact catattgtct ggttcgatga cttctagcca
     3241 ttgatctgcc agaccgccgc caaccatact tgttgctgct ttgataagac ccataaaaaa
     3301 ttcctcctct gattcagcta gtgccaaaca attattgttt atttattata ccataggaag
     3361 atgtgaaact tctcggacta gagacggaat tgaaatggaa aattgcgtat tgtttaaata
     3421 cctgaaaaag ccgagaagga tagtagtttt tttacctaat atcacgtata ataagagata
     3481 gtgaattttt taaaaaaggg ggatccttat ggcatatcaa gcactttatc gagtctggcg
     3541 ttcgcagcgt ttcgaagaca tcgttggaca aaaagctgtg acacagacac taaaaaatgc
     3601 tattgtttcc cacaaaacct cacatgccta cttgtttaca ggacccagag gaaccggaaa
     3661 gacgagtgca gcaaagattt tcgcaaaagc aatcaactgt ccgaacagta aagacggtga
     3721 gccatgcaac gaatgcgaaa tgtgtcgatc aattactgct ggtacgcaag aagatgtgat
     3781 cgaaatcgat gctgccagca ataacggagt cgaagaaatc cgatttatcc gtgatcgggc
     3841 aaattatgcg cctacaaagg cagcgtacaa agtgtacatt attgatgagg tacatatgct
     3901 ttcaacagga gctttcaatg ccttgttgaa aacattggaa gagccaaaag aaagtgtcat
     3961 attcatctta gcaacgaccg aacctcacaa aataccagct acgatcattt cacggactca
     4021 gcgatttgat tttaaacgga tcaatacggc agatattgtc gaacatcttg aatttatctt
     4081 gaaagaatcg actatcagct atgaaccagc tgcattgaat gtgattgccc gttctgcaga
     4141 aggcgggatg cgggatgcat tgagtattct tgatcaggca atctcgttta gtgaaggaac
     4201 aattacttta gatgatgcca tgcaagtgac aggtagctta actgacgaaa tgatggacag
     4261 ttacttgagt gcttgcgtga aaaaagaagt accagaagca ttggaaacat tgaaccaaat
     4321 tctcgcagcc ggcaaagaat cacgtcgctt tctagaggat atgttacaat actgccgtga
     4381 cttgctgatg tatcagcaag cacctaacct agttgaggaa caatcgagta cgatcagaag
     4441 tacatttaca gaattggcac aaactgtttc agcggatcag ctgttttcaa tgataaagat
     4501 tttgagcgat actcaaaatg aaattcgttt taccaatagt gccgctattt atttggaagt
     4561 cgctacagtc aaacttgcac agctaccgtc tgcttctgtt ccttcagcag atgtagcatt
     4621 tgcagatgat cagatagtaa aacagctaca acaagagatt aaggaactaa gaaacgaaat
     4681 caaggaacta cgcagtaata caggaattga gaatacaaaa gtacagccgg tacagcaaac
     4741 aaatcataaa aaacagacaa gtacctatcg tgtaccgacg gaacgagtat ataaagttct
     4801 gcgggaagcg acaagacagc atctgaacca agtaaaagaa gtatgggaag acttgctgat
     4861 gagtctatct gtcactcagc gagcaatgct taaagcaagc gaaccagttg cagctggacc
     4921 tacaggacta gtgattgcgt ttgattatga gattgtttgt caaagagcag caaatgacga
     4981 agaattgcag ctttcggtac acaacaactt aagccgaatg atcaaagatt atgcgcctga
     5041 tcctgtttat attacaagag aaagctggcc agaacttcga agaaattatt tgacgcaggc
     5101 acaagaagag ggaacagaaa actcttttgc ttctgaagac tcagaaggag aaatcgagtt
     5161 gcttcctcaa gaacaagaag taccagaaga aacacttgta gtaaaagcag aggaactgtt
     5221 tggctccgaa gctgttacag tggtagatga ttaataaaaa ggagtagata attatgatgc
     5281 gtggaatggg caatatgcaa ggcatgatga aacaagtaca aaaaatgcaa aaagaaatgg
     5341 cagaagcaca agagctgcta aataaaacag agtttacagg taaagccacc aatgaattgg
     5401 tcaaagcggt ttttacaggc gatcgtcgca tgaaagatat ccaaatcaat gaagaagtcg
     5461 ttgatcctga tgatatcgag atgcttcagg atcttgtcgt aatggcagtc aatgatgcga
     5521 tcgcacaaat tgatgtggaa actgagaaaa aattagggaa atacgcaaaa ggtttgccag
     5581 gattttagtt ttaaactgaa ggagtaatga atatgcaata tccagaacca attgcgaaac
     5641 tgatcgacag ctacatgaaa cttccaggaa tcggtcagaa aacagcgaca agactcgcct
     5701 tttatacgat cgatatgaaa gatgaagtag taaatgaatt tgctaaatct ttgttaagtg
     5761 taaaacgcga tttgcatttt tgtagtatct gcggaaacat cacagaagaa gatccctgcg
     5821 aaatatgtag aaatccttca cgtgatcgaa gcgttatcct agtggtcgaa gagccgaaag
     5881 atgtaatggc aatggaaaaa atgcgagaat atcacggact ttatcatgtt ctccatggtg
     5941 tgttatcacc gatggaaggg acaggtccag aagacatcaa tatttcttct ctgttgcagc
     6001 ggctgcatga tgcagatgta aaagaagtga ttatcgcaac aaatgcaaca acagagggag
     6061 aagcaacagc tatgtatctc tcacgtctga tcaaacccgc aggtatcaaa gtgactcgtc
     6121 tagcacatgg tctgtctgta ggctctgata tcgagtatgc agatgaagtt actttattga
     6181 aagctgtcga aggaagacgt gagttataaa tttctaatta agtgtttttt cagatacaat
     6241 ataaaagcag cacaagagat gggggcaaca atgtgaacgg actatttatt acaatcgagg
     6301 ggcctgatgg tgcaggaaaa accagcatct tgaatgaact ttaccctctt ttgaaagaag
     6361 tagcaaaaac agaaatcatt caaacaagag aaccaggagg cattccgatt gctgaagaga
     6421 tccgagcagt gatccttgat ccaaaaaatg atcggatgga tgaacgtacg gaagcactgc
     6481 tttatgcagc agcaagaaga caacatctag tcgaaaaggt tctgcctgct ctggcaaaag
     6541 ggaaaatcgt tttatgcgat agatttgtcg acagttctct tgcttatcaa ggtgcaggcc
     6601 gacgcatcgg cgttaccgag attgctcggt tgaatgagtt tgctacagaa ggtactacgc
     6661 cggattttac cctttatctt gacgtagatt cagacaccgg attaagaaga atcaagaaaa
     6721 acagacaaaa tcaaatcgac cgtttagatt cggaaggttt ggaattccac cagcgtgtac
     6781 gtcatgctta cttaaagctg gcagaagaga atccggaaag aatccacaaa gttgatgcaa
     6841 gaaaaagctt tgaagaagtg ctgcaaacaa gttatcatac aattattgaa cagtatccgc
     6901 aattttttga aaactaggaa ggtgaacgtt tatgaaaatt attttagcga ttatccaaga
     6961 caaagacagc aaccgtttat caaatgaatt gattgatgca aatatccgtg ccacaaaact
     7021 atcatcgaca ggcggcttcc ttaaggctgg aaacagtacg tttatcgtgg gtattgaaga
     7081 tgaccgtgta gaagaagcat tggaaatcat caaaaaaacg tgtgaatcaa gaaagcaatt
     7141 tgtatctaca cctgttacac tggatatttc gatggatggg ggcgtccctt atccagtaga
     7201 agtagaagta ggcggtgcca cagtcttcgt cttacctgtt gaaggattcc atcaatttta
     7261 ggaggagctg agctatgaat caagaaataa acttggctca aatgcaaccg atcgtgtatc
     7321 agcagcttca acggagtttt gagcatgggc gtatcgctca tgcttatctt tttgaagggg
     7381 agaaaggaac aggaaaacat gaagtaggta tatggcttgc acagcacctg ttctgtaccc
     7441 aaatgaaaga caatctgcct tgtggaaaat gcaacaattg tcagcggatc gaaaagaaag
     7501 aacatccaga tgttttggtt attgaaccgg aaggacaaac catcaaagtc gatcagatca
     7561 gacgtttgca gacggaattc agtcgcagcg gttacgaatc aagaaaaaaa gtttttttga
     7621 tcaaagaagc tgaaaaaatg aatagcagtg cggcaaacag tttgttgaaa ttcttggaag
     7681 agccccctgg agatttttta gcggtgttag aaacagatgc agtaggaaga attctcccta
     7741 ctattcaatc tcgttgtcaa gttctgcatt ttcaagaact ttcgaaagaa gcactgatcc
     7801 atcgattgca agaagagcag attccattgg aaaaagcaaa acttttggct ttcttaacaa
     7861 acagtttagg aaaagcagtt gaaatctctc aagatgaatg gtttaatgat gctaaggact
     7921 tgatttatca atggttcgtt tacttgcaaa agcaagatac tcaagccttt atctatgtcc
     7981 aaaaaaaatt ggtaaaatca tttaaagaaa aaacacaaca gtttacagga ttatctgtct
     8041 tgatgtttta ttatcaggaa gcacagcgtc aagcagtagc aaacggcgaa tatggcaaag
     8101 tgaaacagat aaaccagata attgaacgga tattgatcgc agagcagaaa ctacgcagca
     8161 atgtcagttt tcaggctgtt gcggaacagt tcgttttgca tacgattttt tagcttgccg
     8221 caaaggatga tgatcaagga ggatggaaat ggtagaagta gtaggtgtcc gatatagaga
     8281 tgcaggtcat atctattatt ttgcaccagg agactctgaa tatgtttata gtgacaaggt
     8341 attagtcgaa tctcagcaat ccttacatat tgctactgtt gtccttccta aaaaagagat
     8401 tgatcccaat gaccttcctg aagctatccg tccgatcttg cataaagcgt cacaagctga
     8461 attagaaaaa gacaaaaaaa acaaaaaaga tgcttatgat gcattgaaaa tcgcaaaaga
     8521 aaaagtgcag ttgcgtgagt tggaaatgaa aatggtcaaa gccgaataca catttgatcg
     8581 aaataaattg attttttctt tcacagcgga cgggcgaatt gactttagag aattggtaaa
     8641 agatctggcc gctgtatttc gtactcgtat tgagcttcga caaattggtg tccgcgatga
     8701 agcaaagata ataggcggaa ttggtccatg tgggcgtcca ttatgctgtt cttctttttt
     8761 aggcgatttc gtgcctgtat caatcaaaat ggcaaaagat caaagtttat ctttgaatcc
     8821 aacaaaaatt tccggtttat gcggacgctt gatgtgctgt ctgcaatatg agagcgaaga
     8881 atacgagaca gcgaaaaaag aactccctga ctttggaaaa gtaattgcta cacctgatgg
     8941 taaagggaaa gtcgtagggt tgaatctgct tagccgtatc gttagtattc ggctgactgg
     9001 acgggaaaat cctgtcgaat atgaatggga tgaattgaaa gaatttatcc aagtaggtga
     9061 gatgaatgga taagagatca ttatacgacg gattgaatca attagaatca gagttgagaa
     9121 atacattaac agaacttgtt gaaatgaagc atgctttgca tgagatcgta gaaaaaaata
     9181 caactttgga gatggaaaat caacgtctgc gggaacacct gcgggaattg aaccaagttg
     9241 cacaagctcc agctgaaaat gtgaagcagg aactttctaa gtctcgtatg aatcttgaaa
     9301 aaatttatga agaaggattc catgtctgct acgatttata tggatcaaga agggacaatg
     9361 acgagccttg tgcgttttgt ctagaagtga tctatcgcga aagcggtcga tgaccaataa
     9421 caagaggaga ctggagcgga gaaatactgc tccagtcttt ttattaagga gattgtgaca
     9481 gatgtataat caaaaaagtt tcaagggaaa tccggtatat ggggatttat atcttgtacc
     9541 gacgccaatt ggaaatttag aagatatgag ctacagaagt gttcgtatgc tgcaagaatc
     9601 cgatcttatc gcaagtgagg atacgcgtaa tacacaaaag ttattgaatc attttgaaat
     9661 ccatacaccc caaaaaagtt tacatgaaca taattataaa gaacgtgtcc ctcaattgat
     9721 tgctttgtta aaagaaggaa aaacgattgc tcaagtcagt gacgcaggaa tgccttccat
     9781 cagcgatcct ggacatgagc tagttttagc ctgcataaaa gaagggattt ctgtgatctc
     9841 tatcccagga cctacagctg ggatgacggc tttgattgct tcagggctag tgcctcaacc
     9901 atttttattt tatggcttcc ttccacgtaa gaaaaaagaa cagaaagaaa cattgaaaaa
     9961 gctaaaagaa cagacagtta cgctgatttt ttatgagtca ccttatcgga tcactgctac
    10021 attgacgaat ttcttagaaa tttttggaaa tcgccaagtc gttttgtgtc gggaattgac
    10081 aaaggtccat gaagaatacc ttagaggaag tacggaagag ctactcgctt atatcgaaga
    10141 acatccagta aagggtgaat gttgtctgct tgtagaaggg aatttgttcg aaatgccagt
    10201 gacggaagtt gatgaagagt tgggaacgtt aaaggaacaa gtagaagcaa agatcgcttc
    10261 aggcgaaaaa ccaaatgctg ctatcaaagc agtagcgctg aaaaatgggc tgaaaaaaca
    10321 agaagtctac cgtcagtacc atgaattaga ctgagatagt ataagttgtc tataatgaaa
    10381 gactaatgag gaaatcagct actaactttt caaatctctt gaacgcgaac gaatgttctt
    10441 gtataatgaa taagaggtga ggatgatgga gctgcaattt cctttacata atgatacatc
    10501 aagaaaaatc attcatattg atatggatgc ttttttcgct tcggttgaag aacgagatca
    10561 tcctgagttt cgcggacatc cattagtaat tgccagacac cccagtgaca ctggtgggcg
    10621 tggagtagtg actacagcaa attatgaagc aagaaaattc ggaattcatt cagcaatgag
    10681 tgcacaaaaa gcgtatgagc tttgtccgca agccatcttt aagcctggcg accatcataa
    10741 atacagcgat atttccaaac aagtacggga agtattctat cgttatacag atattgtgga
    10801 accagttagt atcgatgaag cgtatcttga tgtgaccgaa aataaaatca attgcaagtc
    10861 agccatcaaa atcgcccgaa tgatccaagc agatatttgg gagagtgtcc agctaacatg
    10921 ttctgcggga gtaagttaca ataagttttt ggccaaatta gcatcagact atcaaaaacc
    10981 tagaggaatc acggtagttg cccctcaaga cgctgtgcca tttttaaaag ctttgccaat
    11041 tgaaaaattt catggggtag ggaagaaaac tgtacctaga atgcatgagc tagggattta
    11101 tacaggaaaa gacctatatg aatgcacaga aatgatgttg attcgaaatt ttggaaaaat
    11161 gggctattct ctttatcgta aagtcagagg aatccatgat tctccagtaa acgttacaag
    11221 agagcgaaaa tctgtgggaa aagagcatac ttatgggcag ccgttgcaaa cggaagaagc
    11281 agtgctgact cagttgcgcc aactagctga aaaagtagaa gaagcactga gaagagtcca
    11341 aaaacatggg aaaacagtag ttttgaaggt caggtacacg gactattcta ctgtgaccaa
    11401 aagagtgaca ttgccagaat atatctataa aaaagaagca ctcttttatc aagcaagcct
    11461 tatatgggag gaaatcttgg gagtagaaaa aggaatccga ttgttaggga ttactttgac
    11521 gaatctggac ccgatgactt atgaaaacat cgtccttcca ttatgggaaa atcaagaaat
    11581 ataaaaacag ctttgagagt tgaaaccgg
//
LOCUS       DAXWLB010000085        11524 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXWLB010000085 DAXWLB010000000
VERSION     DAXWLB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.085"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..460)
                     /locus_tag="ACGE4H_001746"
     CDS             complement(<1..460)
                     /locus_tag="ACGE4H_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFF8365153.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACGE4H_001747"
     CDS             complement(500..1552)
                     /locus_tag="ACGE4H_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8365154.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACGE4H_001748"
     CDS             complement(1565..2503)
                     /locus_tag="ACGE4H_001748"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFF8365155.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="ACGE4H_001749"
     CDS             complement(2954..3604)
                     /locus_tag="ACGE4H_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365156.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="ACGE4H_001750"
     CDS             complement(3704..4213)
                     /locus_tag="ACGE4H_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFF8365157.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="ACGE4H_001751"
     CDS             complement(4576..4959)
                     /locus_tag="ACGE4H_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365158.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(5200..5814)
                     /locus_tag="ACGE4H_001752"
     CDS             complement(5200..5814)
                     /locus_tag="ACGE4H_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8365159.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            5958..6519
                     /locus_tag="ACGE4H_001753"
                     /pseudo
     CDS             5958..6519
                     /locus_tag="ACGE4H_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="ACGE4H_001754"
     CDS             complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="ACGE4H_001754"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8365160.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(7623..8078)
                     /locus_tag="ACGE4H_001755"
     CDS             complement(7623..8078)
                     /locus_tag="ACGE4H_001755"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFF8365161.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(8106..9551)
                     /locus_tag="ACGE4H_001756"
     CDS             complement(8106..9551)
                     /locus_tag="ACGE4H_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFF8365162.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="ACGE4H_001757"
     CDS             complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="ACGE4H_001757"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFF8365163.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10651..11409
                     /locus_tag="ACGE4H_001758"
     CDS             10651..11409
                     /locus_tag="ACGE4H_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8365164.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         3605 a         2421 c         1790 g         3708 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggcgatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc atttcattga
     4981 tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact aataaataca
     5041 aaaatatttt tattgttgga tacagaaatc tatttttaac tggggatatc tttgccagct
     5101 aatatctttg agactacacc tatcttcatc taaatggatc aattactcgc tttttccatt
     5161 gaccttcgtg gtacttctgc tttctgatag taaaaaacat tactgaatga tcttactata
     5221 taacatacag cggagatcta cttctttatt aaggatcttc atgacgttat ctttttcttt
     5281 tttccgttcg aaaccgcgtc gttcataaaa ttgatacgta cagccgctat ccgtgaacag
     5341 aaatacttct tttccttgct ctcggcgttc aaattctttt aataatttac taccgatccc
     5401 cttgatcttt aaatcagggt tggcagctaa aaagacaatt tctccatctg gggaatgttt
     5461 ttttaaatag tctgaaagca attcgtcatt ggtgtcgtca tacccgcctt tgctgtcttt
     5521 cacaaatgca ttttgtaaga aatcaaatag tttaacatat aacttttgac taaatgaatg
     5581 atgctttttt gattttcctt tgatgtctgc taataaaaca cctgccaact catcatccgc
     5641 atataaagca atgatttgtg tcgcgcgatt caattctaga taccaaaagt atcttccata
     5701 aagattcaaa aggagtttgt tcttggtata ccaatcaaag tgcatacctt taatcgcgaa
     5761 ttgaatggct tttttgtagt ctttcttttc cagttgctta aaggctaatt ccatcactta
     5821 ttctcctatt ctttaaatat tgatatgcta tacttatatt ataatagaca aggtgtcggt
     5881 tatctatgtt cagatagcgg tgaaattgtc ggataaccga cacaattcct tgattatgtt
     5941 gattaggagg ctgcaaaatg aaaaaagagg atcgccgcgt caaaaaaaca gaaaaagcgt
     6001 tagcagaagc attatcaaaa ttattagtag ataaaaaaat tcaaaacatc accatccgtg
     6061 aactgaccga aactgctgat cttcaccgtt cgacatttta tacacattat acggatatct
     6121 atgatcttta tgaaaaactg gaaaatagct ttttccaaga cttggatgaa atcatttcct
     6181 acgatacgac acatagctac gaggatctat acgctcaatt gatcgactat gtctatacga
     6241 atacgttcgt atacaagtta ttcgtcatca accacggaga tatccaattt caaaagagat
     6301 taaataatct gatcgaagaa aattatttaa aaatctggct acaagaagac aaaaaaaccg
     6361 tgatcacaga agaaatgcgt tacttcacca tctaccatat ccaagggtgc ctctccgttt
     6421 taaatcattg ggtagaaaag caattttgca tactctaaga aaaaaatgct gaccttcttg
     6481 cgcttattga acgacaacat agaacctatc atgccttaaa aacaaacgaa aaaggaagcg
     6541 tcgggacgct tccaattctt tatttaaaag ctcaaattga ttattaaaaa tcattaggtt
     6601 acaaacttaa ggttatagtt aaagctttct tacaatttaa tagctgttgc tgtttctttc
     6661 aaaacattat tcagctcttc gatgttcttg cgtccttgtg tctgcatcca ttctttggca
     6721 gcatattcac ctttagtaac gaatggttct acaccattcg cccaagttgc gcgcccacag
     6781 agtaccccat tgaatgtaga gccagcctct tttgcaaatt tcaaggtttc ttggaacaat
     6841 tcagcagtta ctcctgcgct caagaaaata aatggcaggt tggtagtctc ggattgttct
     6901 ttgaaataag cagcagcttc ttctttggta taagcaactt catttttagt gtaaccatcc
     6961 acatagttca tattaacagg aacttccact ttcaaaacgt caactccata gcgaggctta
     7021 gaaaattcct tcatcatttc atttacttta tgcggttttt tcaaggcata ttccttcgaa
     7081 gaagtatcag gatcgttggc atcataagaa accaattcta agaaaaatgg caaatcttca
     7141 gcaacacatt cagaaccaat ccgctccata aacgccttct tttggtcatt gatgtcatca
     7201 gattcatcta catcgtagta taacaagaac ttacaagcat cggccccttg ctcctttaga
     7261 cgttttacag accaaataga taaaatatcc ggtaaacgtc ctggagttga agcatcgtaa
     7321 ccagtttttt cataggccaa caacagacca gcatttttat cgcgaacttc ggctgccggc
     7381 aacccatatt ctggatctaa taagattgct gaagcatatg gagtcaattc agaagaaatg
     7441 atttttttga aattgatgat ttgttcatcg gttgctgatt tattttgaaa tttaccgatc
     7501 atttttttca aggcaccacg ttgatcgatt gctaacgcac tgatgatgcc actttcatta
     7561 cttagttgac gaatataagt tttctttttt tctgttaaag gcatgatttc actacttcct
     7621 ttttagtttt cttctaattt ttggtttaaa agttcagcta tttcttcagc agactgggct
     7681 tgtttcagac cttctgtcac atctttgtgc atcaaaagtc tggctacctg agataaaaat
     7741 tttagatggg ttgttccagc ctctgaatct ggaataaaca gagcgattac aaaatccgtt
     7801 ccttctccat ctaaactttt ccaatcgacc ttaccgttta accgaacaat cacaatatcg
     7861 gcatccttga tactagcatc tttggcatga gggatggcaa aaccatccat cataccagtc
     7921 gtgctttctt cttctctttc catcaatttt tcgtaaactg catctcggtc ttcactaatg
     7981 ctcaagacaa ttgactgatc tgcaaggaag ttcattacct catcttgcgt cgtaaaattt
     8041 ttattcataa aaactttatt tgttttgatc aaagtcattt gaaatccttc tttctagaat
     8101 aacctttagt tgttggttgc ttctttcatt tcaacagcag ctgcttctgc ttcacgtttt
     8161 tctacactac gtttttggat caaccctaaa attactccgc caacgattga accaagtaaa
     8221 atcgacccta cccatgcaac cttaccagta acaacaggta atactagaaa acctccatga
     8281 ggtgcgggaa cttggacacc aaacatatat gtcagtacag cagcaattga tgaacctagc
     8341 atcatagttg gcagaacacg cagcggatct ttagccgcaa atggaattgc tccttcggta
     8401 atgtgcgttg agcccaagat aaagttgact aaaccagcgt tgcgatcatt cgtgtcgaaa
     8461 tattttccaa aaaacagtgc ggcaaatcca gtaatcaatg ggggcgcgat acaagctgcc
     8521 gaaacccctg ccataaatgt atggttacct tgtgccaaca acgcagtccc tgtaacataa
     8581 gccgctttat tgactggacc tcccatatca aatgctgaca tacagccaac gataatccct
     8641 aaaattaatg gattagaatt ttggaatcct gccaagaaat tcatcattcc ttggttgact
     8701 gcttccattg gatttgctaa taaagacatg actacaccaa caaacaagac accgatcacc
     8761 ggatataaga agatcgcctt cagaccatcc aattgtttag gcaagccttt catgaccttt
     8821 tgcaatagta agataaagta gcctgctaag aaacctgcaa aaattccgcc taagaaaccg
     8881 gctccgccag aattggctaa cagtccgcca acaaacccaa caaccaaacc aggacgttta
     8941 gcgattcctt cagcgatata agctgctaaa ataggaacca tcattcccat tgccgcatca
     9001 ccaacaccct ttaacatagc ggcagtcgga ttgtaagtag gatcattggg atcaaaagag
     9061 tacacacccc agatggcaaa cgaaattgca ataattaccc cacctgccac tacaaacggc
     9121 agcatatgag aaacaccgtt catgagattt ttataaatac tgtgaccaat gcctgctgca
     9181 acccccatct ccgctttctc accagcagcg tcaactgttt tgttttctgc agtgattgaa
     9241 cctttaccag ccaacgcatc ttcgatcaat tggtcagcat ctttaatccc cttgctgaca
     9301 ggcacatcga ttacacgctt acctgcgaaa cgttctgctt gaacatcttt gtcagctgca
     9361 atgattacag cgtcagcgtt ttttatttct tctggtgtta attcattctc tatccctact
     9421 tgaccatggg tttcaacttt gatagatacc cctttgcgct tagctgcctg ctccaatgct
     9481 tcttgagcca tataagtgtg ggcaattcct gttggacatc ctgttgcggc aacaattttg
     9541 taagttgtca ttttttactc gctccctctt tgatttcgat ttgtttttcc aactctgcaa
     9601 catccgaaaa atctgttaat cctttacgaa aagcggttga acttccagct gctaaacttt
     9661 tcttcaaatt aatatctacc tgttcacctt ttaaatatcc tgcaagaaat gttcccaaca
     9721 aagtgtcacc agcacacgct gtattgacta ctttaccttt aggagaattc ccagaataaa
     9781 cgatatcctt atttaaaaat attcctccct ctcctcctag agaaaggaga acatttttcg
     9841 ctccatcggc aatcaagcgt tcagcataat gaatatatcc ttcacgatca ctaagctcga
     9901 cattatacca agattgcaat tcctcctcgt ttggttttaa taagaacggc tgatacggca
     9961 gacaatcaat tatttccgga tagcttgaat caataatcag atggacctta ttttcaaaac
    10021 aaatacgact gatatcaata agaatttcgg gtgagacccc tcgcggcaaa ctgccagaaa
    10081 cacacaaata atcgtcagct tgcagctttt gtatttgatt tagaaaagtt ttgatttgtt
    10141 cctgaggaat ctcaggtcct ttattaacca atttgaattc ttcattggtt ttattaactt
    10201 gtgtaaaaac gttgattcta gtcattcccg gtacgctaat aaaatcagtg ctaatatctt
    10261 tttgatgcaa ataatcctca atataattcc cggtaaaacc tgctttaaat cctaaagctg
    10321 tactatcgat acctaattgc ttcatgatca atgaaacatt cacacctttt ccattagctt
    10381 gtatatcatc atcgtttgtt cgattgactt gggatggttt catttcatct gtttcgataa
    10441 aaaggtcgat tgccaagttt aaagtacatg tataaactga catttacgct cctcctttct
    10501 tacatattca ttatgtatca agatcgaatg taaatgtttt cataaatgac taaattcatt
    10561 gaaaactttt acaaaatata ttaaataaac ttgaagtaaa ggaaactccc gctttacaat
    10621 agaattatcc taactaatcg gaggcaaaat atggatacaa aattaagtga atcggagaaa
    10681 tatctctggg attttattca aagtcacata ctcgaaatcc cctcctattc cattgtaaaa
    10741 ctaagtgaat atgccaacgt ttcaacagca accgtcgttc gtactatgaa gaaaaaagga
    10801 tatgaggggt ttacgtcttt taaacattcg ttaaaagaaa aagaaaatac aaatatcaat
    10861 tttgccgctc ttgataaagt tgatgaagga ataaagactg ctattcttaa aaatgagcaa
    10921 gaggtaatcc gcaccatcaa tatggtagaa agcgggaata ttgaagatgc tattcaacga
    10981 atcaaggctg ctagaagaat catgattttt gcccgaggtt tttcagaatt gattgctcag
    11041 gaaatgatgg tcaaatttca acttaccggt aaatattgtg agatgcatac tgatcccaat
    11101 atcataaaaa acattagtag aaaattatcc aaagatgaca tcgtgatctt tgtttcacta
    11161 aacggtgaaa caaaggaatt gactaccgca gctgaaaact gttataaagc tgagatcggg
    11221 acaattttgc ttactgctaa tcgtcattca agtttgatgg aacatatcga gattcctttt
    11281 gttggcttta aatcagaagg ctccttcttc ccagattatg aggtacgctc cagacttcca
    11341 ctttctatac tggctagaat cttacttgac tcctatgcac tgcgaacatc aggattcaca
    11401 cagcactaac tctatatctg taagactgta cctaaaatat atctgtgtga attaactaag
    11461 gttgtctaga aaaatattta atcgctattt gtattctcgt gctttctatc attgctcctt
    11521 ctat
//
LOCUS       DAXWLB010000086        11495 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXWLB010000086 DAXWLB010000000
VERSION     DAXWLB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.086"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            218..1192
                     /locus_tag="ACGE4H_001759"
     CDS             218..1192
                     /locus_tag="ACGE4H_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365165.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1214..1906
                     /locus_tag="ACGE4H_001760"
     CDS             1214..1906
                     /locus_tag="ACGE4H_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFF8365166.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            1872..3380
                     /locus_tag="ACGE4H_001761"
     CDS             1872..3380
                     /locus_tag="ACGE4H_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFF8365167.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            3454..4557
                     /locus_tag="ACGE4H_001762"
     CDS             3454..4557
                     /locus_tag="ACGE4H_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFF8365168.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            4560..5897
                     /locus_tag="ACGE4H_001763"
     CDS             4560..5897
                     /locus_tag="ACGE4H_001763"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8365169.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            5884..6762
                     /locus_tag="ACGE4H_001764"
     CDS             5884..6762
                     /locus_tag="ACGE4H_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFF8365170.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            6759..7682
                     /locus_tag="ACGE4H_001765"
     CDS             6759..7682
                     /locus_tag="ACGE4H_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFF8365171.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            7679..8089
                     /locus_tag="ACGE4H_001766"
     CDS             7679..8089
                     /locus_tag="ACGE4H_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFF8365172.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            8093..9661
                     /locus_tag="ACGE4H_001767"
     CDS             8093..9661
                     /locus_tag="ACGE4H_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFF8365173.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            9901..11319
                     /locus_tag="ACGE4H_001768"
     CDS             9901..11319
                     /locus_tag="ACGE4H_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365174.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIDGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         3721 a         1870 c         2462 g         3442 t
ORIGIN      
        1 aatcatttcc tatgctaagt gtcagataga aaaaaataga aggtttgcta tgcgtagttt
       61 atttgtgaaa aaatacactt tatcgttttg tatagcacaa aaatcattgc tatgtaagcg
      121 tttttactta cggtaaaaat gattatatac gtaattcaga tagttatcta tcatgaagtc
      181 ataagcagca aacattcaca aaactaagga ggggcttatg gaaagtgtaa agaaacaaga
      241 accatttcaa ctgacgctat cgaaaaaaga agaaaagctc agaagacgaa aaaaaataat
      301 tgcaggaatc aaaacgaata aatttttata tgtgatgatt ctgccgggag tcctttattt
      361 cttgattttt aggtatcttc ctatgggggg actcgttatt gcttttcaag attatcaacc
      421 atttttagga ataatgggaa gtccttgggt cggtttcaaa catttcattc gtttgtttac
      481 agaaccgaca ttctttacat tattaagaaa tacgttgatc ttgttcggat taaatatctt
      541 cgtgtttttt ccgatgccaa tcattttgtc tttactatta aatgaagtaa cgaacaaacg
      601 attgaaaaac gggattcaaa cgatcattta catccctcac ttcatgtctt gggtaatcat
      661 tgtttcgatt acttatgtat ttttgaatgt tgacggaggg gtattgaatg aattccttgc
      721 aacaatggga tttgaaaaaa tcagttttct aacatcccct gaatggacac gcacagtata
      781 tatcggacaa gtgatctgga aagagttggg ctggtctacg atcatctacc tttcagcgat
      841 aactgcagtc gatacacagt tgtatgaagc tgcagaaatg gatggagcag gacgcttacg
      901 caaaacatgg catgttacat tgccggcaat tcgtccagta atcattacct tgctgattct
      961 aaaaatcggt cagacattag acttagggtt cgagcatatg tacttgttat tgaattcatt
     1021 gaatcgaagc gttgcagaaa ttttcgacac ctatatttat acagctggat tgaagaatgg
     1081 acagttaagt ttcagtacag caatgggatt gtttaaaggg gtcgtaggat tgtttatggt
     1141 aattttagct aataagattg ccaaaaagct aggagaagac ggcgtttact aagatagaaa
     1201 gagagggaaa gatatgctgc agtcattaga taaaataaac ggaaggctaa atcgtatttt
     1261 acagttggtt tatttgaatt ttctgtgggg cggtttagtc atcgcaggtt tgggagtttt
     1321 tactttcggt cctgcatcgt atgcgatggt tgcagtgatt agaaaatggc tgcgctcaaa
     1381 agaaacgttt cctttgacga aaacgtactt cacatatttt aaagaaaact atcgtgaaac
     1441 cttgctgatg agctggatat atggcgccat cgggattgtt ttacttgtgg atctttcttt
     1501 catacacaat tggtatctta ggatcgcttt gtatattgct gcgttcatat atgtgctctc
     1561 agcactctat attttcccaa tcatggctca ttatgattgg aaaggatatt ttttcaaaat
     1621 gaaaatggca tttttattcg gattctcaca tcttcattac tcattagtgt tgatattaag
     1681 tatggtaggg atttatctga tgatcatcca aacaatgcca ggtgttttaa cctttatcgg
     1741 tggcagtctc ttcttttccg cagtcacgtg gacagcaaat tctatatttt gccatttgga
     1801 atggaagaac tcatatacag aaaaaacaga agaaaacaaa aatattatta aaacagtgaa
     1861 ggagaaaatt tatgaaaaaa gcaacgctcg taaaattagg tcttagtttt gcaggaattc
     1921 tagcacttgc cgcatgcggc agcagttcaa gtaacagttc ttcagagaca gaagatggaa
     1981 aagctaaaat cacttggtta aatattttgc atcatgcttc accgcctaca gacacgattg
     2041 tgaacttgat cgaagaaaaa accgatacgg aactgacatt caactggatt cctgatgctt
     2101 caaaagaaga aagattaaca actgcattgg cttctgatga actaggagat atcgttacac
     2161 ttcaaattgc gatgttgaaa aactcttctg tccgcaatgc gttaaaatca ggcgtattct
     2221 gggatgtatc agaatattta aaagactata aaaatttaag tcaaatttct gaagatagaa
     2281 tcgaagctgc gagtatcgac ggtgccttgt acggcgtacc gatacaaaaa gactacgctc
     2341 gtgctggctt agtcattcga aaagactggt tggataatct aggactagat gttccaacta
     2401 ccatggatga gctttatgaa gtagctaaac aatttacaga aaacgatcct gatggaaatg
     2461 gtgtggacga cacagttggt tttggcgatc gtgcgcctag tgatattcgt tataccagct
     2521 ttaaactttt cacttcttat tttggagcac caaatggctg ggctgtagac gataatggca
     2581 aattcactcc agaatttgat acagaagagt atatgaaagc tttgaatttt tcaagagatc
     2641 tatttgagaa tggctatttg gcacaagact ttgcagttac ccaaaagact gatcagcaag
     2701 aacaatttgc acaaggaaga actggaatct acactggaat ggtggatatc aagaatctga
     2761 agacgatggg tcaaggtatt caagatgata tggaattagt gcctgtaaac aagatctcag
     2821 atggtgaagg cggcggttat catgtttggt cagaaggaaa tggtattggc ggcttgctag
     2881 catttcccaa atctcaagta aaagacgaag cacaattgaa gaaattactt caatttgttg
     2941 atgatctttt agaagaagat gtgtataaat tgatgactgg cggtatcgaa ggaacgcatt
     3001 atgaattgaa tgatgatggt tctatcgaat ttatcgatat ggatctttgg caacaagagg
     3061 tgcaaccgtt atcaagctct cgtcctagtg aagtgacgtt ctctttcaaa gatacagacc
     3121 ctgaaaaaga attctctaat caattgatca aagaaaatga agaatttgct gtaatggatc
     3181 cttctgtgcc attggattca ccgacgaaca atgaagtagg atcagaaatt gaaaaaatcg
     3241 tcattgatgc tacggtgcaa tacatcatgg gacagcttga cgaagatgga ttcaaaaaag
     3301 ctgtagaaga ctggaaagca caaggaggcg atcgaatcac acaagagtac gaggaagcat
     3361 acaaagcagc ggcacagtaa tatagacgat tgggacaaat acttcttggt atttgtccca
     3421 aatcctattt taatttttaa aggagagcaa ataatggaaa actggtcaaa gaaattagca
     3481 aaaagtatca tggaaagaac acctcgctta tatgaagaaa agtggtataa aggaaaatgg
     3541 tcttatgact atggcgtcgt acttaaaggg ttccaattac tttgggaacg aacccaagaa
     3601 aaaatttatt ttgatttcat aaaagacaat atcgactatt tcgtgcaaga agatggaacg
     3661 atccgtggct acagcgttga agaatacaat atcgaccatg taaatacagg aaagcttttc
     3721 tttttattgt acaaagaaac aggcgaagaa aaatataaaa aagcagcaga actcctttcc
     3781 cagcagttgg caaaccatcc acgcacatcc gaaggagctt tttggcataa agaaatttat
     3841 ccttatcaaa tttggttgga tggcttatat atgggttctc ctttttatgc tgaatttatc
     3901 cagaatttta gtgaagaaaa agattaccaa gatgttttga gacagtttga aatcagttat
     3961 aaacatctat tcgatccaaa aacaaatttg ctgattcatg catgggatga aaagaaggtc
     4021 caaccatggg ctgatccaaa aacaggtcta tcccatcatt tttggagccg atcgattggc
     4081 tggtatttga tggctgcagc agatacctat gaattacttc atgaagaaac agattgttct
     4141 ttgctgaaag aaattctaga aaagacactg gctgcattac ttccatatca agaagaaaac
     4201 agcgggacat ggtatcaagt aacaacagaa acaaaacgga aaggaaatta tctggaagct
     4261 tccggcagta gtatgtttgt ttacgcaatg gctaaaggaa tacgtttggg actgctgaac
     4321 aaagaagaat ggcttccaca agtgaagaaa tcacatcaag gattacttga tgaatttgtt
     4381 ctggaaacaa aagaaggctg gctgaacttg aataaaaact gtcaagtagc agggttaggc
     4441 ggagcagatc agcgagatgg ttcatatgct tactacatca gtgaaccaat tatttgtaat
     4501 gatcaaaaag gcgttggtgc attcttgcaa gcattgattg aagtggaggc attataaata
     4561 tgtatgatat tttgacattt ggcgcatcaa ttgatgaatt gaatactgaa gcaatacaac
     4621 aagccattga tgctgcagca agtgacggag gcgggaccgt agttgttcca gcaggtgaat
     4681 ttctaacagg agctttattt ttaaaaagca atgtcgaact tcatttaagc gcaggagcgg
     4741 ttctgaaatt ctctgacgat ccaaaagatt atcctgttgt tcattcacgt tgggaaggag
     4801 tccatcgaaa agtttatgct tcctgtatct atgctcaaaa tgtagagaac atctctgtca
     4861 ctggtttcgg tacattagat ggaaatggaa aaaaatggtg gcatactttt agaaatgaac
     4921 cagataatct cgcttatcct cgtcctaaat tgatgagttt ccataactgt catcgaatta
     4981 ctgtgaaaga tattaaattg atccagtcac caagttggac gatcaacccg attctttgca
     5041 gtaatgcaac gtttgataac ttgacgatct tgaacccagc ggattcacca aatacagacg
     5101 gaattgatcc agaatcatgc aaaaatgtgc gtattagtaa ttgccatatt gacgtgggag
     5161 atgactgtat tgcaatcaag gcagggactg aagatacgta cgaaaggatc gcttgcgaaa
     5221 atatcacgat cacaaattgc acgatggtcc atggacatgg cggtgtcgta ttaggaagtg
     5281 aaatgagtgg tagtattcgt aatattacca tttcaaattg tatcttccaa gaaactgatc
     5341 gaggaatacg attgaaatct agacgaggtc gaggcgggat cgttgaagat attcgagtat
     5401 ccaatatagt catggataat gtgatgtgtc catttatact gaatctttat tatttctgtg
     5461 gtccgcgagg gaaagaaccg tatgtatggg aaaaaaaggc ttatccgata gatgaacgga
     5521 caccagcatt cagaaggatc catttttcga atatcacggc aaggaatgtc catgcttctg
     5581 caggttttat ttatggatta gcggaacaat tcatacaaga aatcacattt gacaatatcg
     5641 atgtctcgat ggcccaagat gcagttcccg gtgttcctgc tatgatgaca ggaatagagg
     5701 aaatgagcag acaaggattt tatattggct ttgcgggtaa ggtttccttt aaccagatgt
     5761 cgattgaaaa tcacgaggga ccagcatttc tattagaaaa tactaaagaa atcagtattg
     5821 agaaattcca atcaaatcaa tcagaaaata tggatgaact ggttgttcgt aaaaactttg
     5881 cttatgcagt tgaatgattt tttaggtgct ccatctagca gacatacccc tagtggaaaa
     5941 cgattatacc gagaaaaaca aaacggtttt atgcttgaat actatcaatt gtacttaaat
     6001 gaattggaaa cagtaccagc cctctatgct tatccagaaa aagaaggacc gtttccgccg
     6061 gtcctttacc tgcattctca cggcggagat ttttcagtcg ggaaaagtga attgatccat
     6121 ggtgctcctt acttagcttc tccatctttt gcagaagtac tgacaggaat gggatatgct
     6181 gtttggtcga ttgatgcttg gggatttgaa gaacgcggag gtatttctga aagcgagttg
     6241 ttcaaacaat ttttattaac aggaaaaacg ttatggggaa tgcgtattta tgatgtgatc
     6301 agcttgatcg attatttgga gacaagggaa gacacagata ctcaacggct cgcaacgatt
     6361 ggtctttcta tgggaggctt attaagctgg tgggtgacag cattggattc tcgtgtcaaa
     6421 gtttgtattg atctagcggc acaggtggac atcgaaatac ttttgctaca tcgccgccta
     6481 gatcatcatg ggttttatta ctatgtgcca aatctattga cagcttatac aacgttagca
     6541 atccaagaaa aaatcgctcc gcgccctcgt cttagcttag ttggaaaaaa cgatacgatg
     6601 tgcttggacg aaggcgtact gaagctgaga aaagggctgg acaaaaagta tcaatcaatg
     6661 ggtagtccag aaaacttttc ttctttttct ttaacaggcg gtcatatgga aacacaagaa
     6721 atgagaaata tttggaaaca attcataagg gagcatttat gaggatgaaa gacaaactga
     6781 taattggaaa gaatgctata gataccccga cggattttga aactatccaa gaaggaatcg
     6841 attatttggc aggcctgccg gatagccatc caaaggtctt gatagtaaaa gaaggaagat
     6901 atgatgaata tatagaaagc agattatcca attttaaact tgtgggagca ggaacagtag
     6961 ttatctccgg taatcgtttt gcaaaacagg agtatcttga cggtagccag ttagggactt
     7021 tccgaagtgc tacttttttc ttagaaggat cgaatgtcgt attagaaaat cttcacatcg
     7081 aaaatacagc aggtccagga gaaaaggtag gacaggctgt tgctttattc aattcaggag
     7141 atcgcgtatc tttaaaaaac tgtcgactat ctggttatca ggatacctta tgcacgggtc
     7201 cgttgcctga tttgcaaaaa gatggtactc ctttttgtac accggcttct gccaatcctg
     7261 agtattgtcg tcagacctat gaatattgct ggattgaagg aacggtggat ttcatatttg
     7321 gtggggcaga tgccgttttt catcagtgtc atatccatag cttagccagt aaaaatcctg
     7381 gatacatcac agcagcatct acgccgaaaa atcaaaaaac agggtatcgt tttgaaagct
     7441 gtaccttgac tgcagaacct gatactagaa acgtctattt aggaagaccg tggcgtccgt
     7501 atgcaaatgt cttatttgaa gcgtgtaaga taggtgggca tgttcaccca actggttggg
     7561 ataattggga tgacaagaaa aatgaacaga ctgtcaaatt ctctgaacgt aataacaatt
     7621 atgaaacatc gatcaaaaga cctgaatgga tacatttaga aaagcagggg gttcagaaat
     7681 gaaaagaaaa tgggaactat tgctaggaat ggtcggtggc agtctatctt tgattttctt
     7741 tggcgggtta gcagttactt taagtaatat gtctgcgtca gagtttaaaa aaagttatca
     7801 gtcgcttgct gtagatcatt caactttgtc tttagaaaat acatttggat tattacagga
     7861 catgactggt ctgtttgccg ttgtgttgtt tatttcactt gcttttttag cagtagcatt
     7921 gtttttgact gctaaaggaa aatacttaac aactgccact ggtttgtatt ttataacagg
     7981 attcatattg ttgattggca ctcagtttat tgcgtttcct tttgcatttt tttatttcgc
     8041 agctggggca ttcagtttat atcgagtaag aatgagaaaa ggagcctgaa tcatgcagac
     8101 tatcaaagtg aaaaatccta ttttaagagg attcaatcca gatcctaata tattaagagt
     8161 gaaagagcgg tattacatta ttgtctcctc ttttgaatgg cttccaggaa tccgagtata
     8221 tgagtcgaaa aatttagtca actggtcaca tacaacggat attttgacca ctcaagtatc
     8281 tttgcaagga aatcctacaa atggaagtat atgggcgcca cagatcagtt atgctgatgg
     8341 attgttttat cttgtttaca cagatgtcaa aagtgcaggc cgtccgttta aagatgtcca
     8401 caattatttg atcactgcat ctgagatcac aggtccttgg tccgaaccaa tttatttaaa
     8461 tagtaacggc tttgatcctt ctttgttcca tgatcttgaa acgggaaaaa aatggcttgt
     8521 caatgaacta tgggattatc gtctggacac accgaataaa tccagcggta tcgtattaca
     8581 agaatatgat gcagagaaga agaagcttga tggtcccatc tataaaatat ttgcaggtac
     8641 agaattagcg aaaacggagg caccacattt gtattttcgt aacgggtatt attatttgat
     8701 gacggcagaa ggcggtacag gaagaggaca ttcagttaca gtttgccgag caaagaatat
     8761 tactggtcct tacgagcttg atcctggttt tcctatgctg acagctagtg acaaacctgc
     8821 ttctacattg caatgtacag gacatgggag cttagtacaa accccgtcag gaagatggta
     8881 catgacatat ctttgtacca gaccattaaa cggtgcggct attttgggaa gagaaaccgc
     8941 tattcaagaa gtgtattgga cgtccgacga ttggttaaga cttaaagata gccagacaac
     9001 tccgatggaa gaaataatga ttgagacaaa tgaagaggta caacaagaaa aagatcatca
     9061 gtttatggat acatttgaag gagagctaaa gaagaactgg aatgccagac gcattttgcc
     9121 aaatgctgat tggtgtgata caaaatgtag accaggtttt ttacgtctga tttccggtga
     9181 atctcttcaa tctaccttcg aacatcattt gttagctatt cggcaaacgg attttacttt
     9241 tgatgcggag acagcatttg aatacatgcc taataatttt aatcagatgg ctggactggt
     9301 actgtactta aatgaatcca attatattta ttgttatgtt acttggaatg aggacaaggg
     9361 aaaatgccta cgtatgatag aatcagaaaa cggagtagca caaatagaag attggacgat
     9421 tccactcaca gaaaagaaga cttttttgaa agtcactgta aagaaagaga cagcacagtt
     9481 tcatttgaaa gacgtatcaa ctgaagaatg gaagaaagta ggtccgcaaa aaaacatgtg
     9541 tatactctca ggagggttta ctggaaattt tatcgggata gcagtgcatg atctaaataa
     9601 aaaaagagga agttatgctg attttgagtt tttcaagtat gatggaaaag atcatctgta
     9661 atttaattct ttaataaatg aggttcacta acttattttc actagttagt gaattttttt
     9721 gtttgtatat attgttatgt aaaaaaagtt atcaatctta tgcatcaatg tttttagatt
     9781 attttttgtt ttttatctgt caagataatg caataaaaag ccaacattgt gaatataaaa
     9841 acaagaaaaa aagagttata attttgaaaa cgtttactag aagaggtagg aggaattcaa
     9901 atgctttcac aaaatgctgt tacagcaaat acaataaaaa aagaaaaaaa ggaatttggg
     9961 atttccgata agataggcta catgttcggt gatctaggta actgttttat tttaggttta
    10021 gtcaatagtt tcttgatgat ttattatacg aatgttcttg gcgtttcagg ggcaatcgta
    10081 ggtacgttat tcttgatttc gagaattttg gatgcgtttg ctgatgtgat ggttggaaga
    10141 ttatgtgatg tctcagagtt aacagaaact ggccgattta ttccttggat cagaaaaatg
    10201 aagtatccat tttgcttagt gactgtcatt ttgtttttgc cattcgtcca acatcttcca
    10261 atgacggtga agattgctta tatttttgtt tcatacattg tttatgggat ttttctctca
    10321 acaatcaata tcccgtatgg agcaatggca gcagcaatta gctcaaatcc taatgaccgc
    10381 acctcgttgt ccacgttccg aagcattggt tctgcgattg acggatcaac tacaggattc
    10441 ttcattccga ttttaatgta tacccataac aagagtggac aacaagtcgt ttcagggaat
    10501 catttctttt ggatttcaat cggatgtgct gcaatagcat ttgtcgcata tatgttaact
    10561 tgtaaattga ctactgaaag agtaagagtt gtcaaaaacg aaaaagtatc tgcttctcag
    10621 ttggtaaaag gtttgttagc taatcgagca ctgattattt tagtcattgt tgacattttc
    10681 attgtgatca atcagatttt agctgggacg aatatgactt atttattcaa tgattacttc
    10741 cataataaac aagcaatgtc tgttgctctt ttatttactt acggaacagt agtagtttta
    10801 gcaccatttg ctacaaagct gacaaaatta tttggtaaaa aggaagcaag cgtatttgcg
    10861 ttacttgctt cttcatgtat gtacttgctg atgtatttcc ttcatataac aaatgcatgg
    10921 gtatatctga ttcttttatt tatcgctact ttaggatctg gtttattcaa tttgatggtc
    10981 tgggcattca ttactgacgt gattgattat catcaatata agacaggctt aagagaagat
    11041 ggaacagtat atggagtaaa ttcttttgcg agaaaagtag gacaagcatg tgcaggagcg
    11101 gtaggcggat ttatgctggc actcattggt taccaatcgt ctacaacagg tggagccatc
    11161 caatcagttt tagtacaaga aaggatttat acgatggcaa acttgttacc agcattctgc
    11221 ttatttgtat ccgcaatcat tttgctagta ggttacccac tgaatcgttc aaaaactgta
    11281 tttattggtg aagaattgaa aaaaatcaat gaaagatagg gagaaacgta ttgcggacaa
    11341 aaaaagggat tatcaatgct ttgaacttta aaacaaacga cactcaacaa gatgatacaa
    11401 taatactgca aaaagcaatc gatcaaggtg cgaaagaaca cttacctgta tttattccaa
    11461 aaggcattta tttagtcgga gccctttttt taaaa
//
LOCUS       DAXWLB010000087        11260 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXWLB010000087 DAXWLB010000000
VERSION     DAXWLB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.087"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(279..494)
                     /locus_tag="ACGE4H_001769"
     CDS             complement(279..494)
                     /locus_tag="ACGE4H_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365175.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="ACGE4H_001770"
     CDS             complement(546..1460)
                     /locus_tag="ACGE4H_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365176.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="ACGE4H_001771"
     CDS             complement(1460..2359)
                     /locus_tag="ACGE4H_001771"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFF8365177.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="ACGE4H_001772"
     CDS             complement(2359..2946)
                     /locus_tag="ACGE4H_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365178.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="ACGE4H_001773"
     CDS             complement(3110..3301)
                     /locus_tag="ACGE4H_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365179.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="ACGE4H_001774"
     CDS             complement(3374..4276)
                     /locus_tag="ACGE4H_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365180.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="ACGE4H_001775"
     CDS             complement(4293..4769)
                     /locus_tag="ACGE4H_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365181.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="ACGE4H_001776"
     CDS             complement(4785..5384)
                     /locus_tag="ACGE4H_001776"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFF8365182.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5943..6422)
                     /locus_tag="ACGE4H_001777"
     CDS             complement(5943..6422)
                     /locus_tag="ACGE4H_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365183.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="ACGE4H_001778"
     CDS             complement(6441..7070)
                     /locus_tag="ACGE4H_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365184.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="ACGE4H_001779"
     CDS             complement(7067..8443)
                     /locus_tag="ACGE4H_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFF8365185.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="ACGE4H_001780"
     CDS             complement(8523..9785)
                     /locus_tag="ACGE4H_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365186.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="ACGE4H_001781"
     CDS             complement(9798..10145)
                     /locus_tag="ACGE4H_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365187.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="ACGE4H_001782"
     CDS             complement(10165..11034)
                     /locus_tag="ACGE4H_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFF8365188.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
BASE COUNT         3154 a         2272 c         1514 g         4320 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgtcaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttcctttgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct
//
LOCUS       DAXWLB010000088        11249 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXWLB010000088 DAXWLB010000000
VERSION     DAXWLB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.088"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(5..2014)
                     /gene="metG"
                     /locus_tag="ACGE4H_001783"
     CDS             complement(5..2014)
                     /gene="metG"
                     /locus_tag="ACGE4H_001783"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFF8365189.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2282..2560
                     /locus_tag="ACGE4H_001784"
     CDS             2282..2560
                     /locus_tag="ACGE4H_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365190.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2657..3478
                     /locus_tag="ACGE4H_001785"
     CDS             2657..3478
                     /locus_tag="ACGE4H_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8365191.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3763..4773)
                     /locus_tag="ACGE4H_001786"
     CDS             complement(3763..4773)
                     /locus_tag="ACGE4H_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8365192.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4785..5738)
                     /locus_tag="ACGE4H_001787"
     CDS             complement(4785..5738)
                     /locus_tag="ACGE4H_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365193.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5728..6798)
                     /locus_tag="ACGE4H_001788"
     CDS             complement(5728..6798)
                     /locus_tag="ACGE4H_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365194.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6804..7847)
                     /locus_tag="ACGE4H_001789"
     CDS             complement(6804..7847)
                     /locus_tag="ACGE4H_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365195.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7848..8789)
                     /gene="opp3b"
                     /locus_tag="ACGE4H_001790"
     CDS             complement(7848..8789)
                     /gene="opp3b"
                     /locus_tag="ACGE4H_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFF8365196.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8873..9220
                     /locus_tag="ACGE4H_001791"
     CDS             8873..9220
                     /locus_tag="ACGE4H_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFF8365197.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9290..10951)
                     /locus_tag="ACGE4H_001792"
     CDS             complement(9290..10951)
                     /locus_tag="ACGE4H_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8365198.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            11130..11249
                     /locus_tag="ACGE4H_001793"
     CDS             11130..11249
                     /locus_tag="ACGE4H_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFF8365199.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3174 a         2287 c         2046 g         3742 t
ORIGIN      
        1 atttctatgc gatttctgag ccatttggca tggattttgg tgcttcgatt acttgtaaag
       61 agccatctgg tgcttctgca gaaaggatca ttccttgact gatttgtcca cgcatctttc
      121 gtggtttcag atttgctaca atgacgactt ttttcccgat caattcgctt ggatcaggat
      181 aaaattcagc gattcctgac aagatttgac ggtcttggct gtctccagca tctagtcgga
      241 attgcagcaa cttatctgcc ccttctactt tttgacagtt gatgacttct gctactttta
      301 gttctacttt ttcaaataca tcaaatttga tttgtttttc tttcgttgaa acaagttctg
      361 tttcttctgg atcccatttg actgtatctt cgtttgtttg agtaccttca gacatttttt
      421 cttgaataaa agcaacttct tcttccatat ctaaacgtgg gaagattggt gtaccttttg
      481 caactacttt tgttcctgaa ggaaattcgc caaagtgtaa tccttcaaga ttcattgttt
      541 cagagtccaa tcctagttga ttgaagatct tctctggcgt ttctgtcatg attggctgaa
      601 gtaatatcgc tgcgatacgc aaagattcag ctaaatggat catgacacta ttcaattcag
      661 ctttcttttc ttcgtctttt gctaataccc atggttctgt ttcatcaatg tatttgtttg
      721 cacgagaaat cagcacccag atttcagcca atgctgtatt gaattccatt ttttccattg
      781 catcatggta acgtccgaca acgttagcag ccgtcgtaga cagttcacta tcaaatggag
      841 tgacaagtga ggcatagtca ggaacgagtc catcacaata tttgttgatc atagcaatcg
      901 ttcgattcaa taagttccct agatcatttg ccagatcata attgacacgg gaaacaaaat
      961 cttctggcgt aaagacacca tctgacccaa acggaacagc acgaagcaaa taataacgga
     1021 gtgcatctaa accataacgt tccacaagca tttcaggata aacgacattt ccttttgatt
     1081 tagacatttt tccgtctttc atcaatagcc agccatgccc aaatactttt ttaggtagag
     1141 gcaaatcaag ggccatcagc atgattggcc aatagatcgt atgaaagcga acgatttctt
     1201 taccgaccat ctgaacatct gcaggccagt atttttcgaa tagactttca tcttcggagc
     1261 catatcctag tgcagtaata taattggata atgcatcgat ccatacatac accacatgtt
     1321 tcggatcact cttgactggt actccccaag taaaagttgt tcgtgaaact gcaagatcct
     1381 ctaatcctgg tttgataaag ttgttgatca tttcgttttt acgagattct ggctggatga
     1441 attcgggatg ttcttcatag tattccaata gacggtctgc gtatttgctc atgcggaaga
     1501 agtatgattc ttcttttacc aattctactt cgtgtccact tggcgcttta ccaccgatca
     1561 cgttaccttc gtcatctttg tacacttctg caagttgtgt ttctgtaaag aattcttcgt
     1621 cagaaacaga gtaccagcct tcatattcac ctaagtaaat atctccctgt tctaataaac
     1681 gatcaaatat tttttgaaca gctgctttat ggtaatcatc tgtcgtgcga atgaatttat
     1741 cataactgat atcaagcgtt ttccataatt tcttgacatc agccgccatt ttatctacat
     1801 attcttgcgg ttttacacct agttcttctg cttttgtttc gattttttga ccatgttcat
     1861 ccactccagt gagataaaac acatcaaagc ccatcaaccg tttataacga gcaattgcgt
     1921 cacatgcaat cgtagtatac gagttgccga tatgaagttt gccgcttgga taatagattg
     1981 gcgtagtgat ataaaaagtt tccttttctg ccacggggct cttcctcctt tatcgcatga
     2041 tgcgaattcg tttccttgct agtataccat aaaaaaagca tgctgttagc tttttttcaa
     2101 aatgaaaaag actggataaa cgacttctat ctacgttcaa tctatccttt aaaaggatga
     2161 ataggttgaa taaatgcggg tttttgtttt gtttcatatc tactaaaaaa cggcttttta
     2221 tttctcccct cacacgtggt ataataaaga ctatctatct tagtaaggga ctggaacaat
     2281 catgcagaag aaacatcttt tttttacact tagtattgct tttctttcat tagcacattt
     2341 aatattcagc tatttttata ttcgtatgta tggttatttt aacctccatg ggcatttgaa
     2401 cagttttatg acagcagctt ggattttgcg ttttatcatt gacgtatata tcgttatttg
     2461 tggcttcttt gccattcgtg aagagcggta taaagtattg ccgttttatc tgcttttctt
     2521 tttattcaat ctcatacttc catttatttt tcatatttaa atgataacct agatttttca
     2581 agtaaatcct tggttttttt tcagagtctg ctacaataag gtaaaaagta cttttactgt
     2641 caatggaggt ggtcagatga accgttttgc ttctaaagaa gaagaaaaat tatattatga
     2701 acataaagca agtgaagaag aatttcttaa ctggtatcaa aaacaggaac gtccagaata
     2761 tgacaagccc tctttgactg tcgatatcgt actgatgtgc tataacaaag aagaagatca
     2821 gctgaaagtt ttgttgatcc aaagaaaagg gcatccttat cgaaattcat gggcattgcc
     2881 tggtggcttt gtccaaaagg atgagtcaac tggagaaagc gtcttaagag aaacaaagga
     2941 agaaactggt gtggttattt caaaagaaaa tattgagcaa ctgcatacat tcagtacgcc
     3001 taatcgagat ccgcgaggct gggtcgtcac agtcagctac ttagccttta ttggagaaga
     3061 accgctaatc gccggagatg atgccaagga agtacgctgg ttcacactag aacgtcatag
     3121 taacaagatc catttatcta gcggagaagt ggctatctcg cttgatttag tcactggaga
     3181 gtcttctggc aaagatactt tggcttttga ccatagtcaa atcatcttga aagcattcaa
     3241 ccgtgtagtc aataaaatgg aacacgagcc gcaagttcta caagttttag gtaaagattt
     3301 cacgattacg gaagctcgaa aagtatttgc taaattctta gggatcgatt ataaaacgat
     3361 cgatcattcc aattttaaga aagctatgct ccaatatttc gatgagatcg gtgaacgtcc
     3421 tgtaggaatc ggccgtcctt caaaaatcta tcagctcaag ccggggcacc atgaataagg
     3481 ttgtgagcaa aagattcaga cctaatcatt caagaaaaaa cgcaaaaaca actctttgtg
     3541 agttacgttt tttgtttcag aattttgatg tattcgtaaa ctttctgaat aaaaaattaa
     3601 caatagcaga aaaagctcgt acgaactttt tctgctattg ttttattggt ttgtcataat
     3661 tagtgagctt ttcttcaaaa gaaaaattcc tagaaacaaa agtcattaag actttttatt
     3721 aacaaggcta accctaacgc tcctatcagt ttcattcatt ttttatccct tgataaccga
     3781 atgtctgccc attttttcta ctacttggct ctttcttacg gaattgcttt cttctgctcc
     3841 gtcggtattg atcaaaagaa attttttatt ataccattgg gacttataac gcaattttgt
     3901 tattaattta tgccagtcgt gaatagaaaa atcagcatgt gcctcttctt ttggaaaaga
     3961 ttctacaaat aagtagtcat attgtttgac caatttccca gataattgtt caagataatc
     4021 ttctcgctgg ttcttttgtt tcagataaag atccattact tttctctttt gtttctgata
     4081 attgcttgcc tgatctaacc tgatctttct atggtgagcg gagcggactt tcacattcaa
     4141 tttccgctgt gcatgttgca gcttttcttc tgtctggtga aattttggta aagttacttc
     4201 tagaggtcga gatgtttgga tcaatgtctt cggatgatag gcgatcccga tccatttagc
     4261 taaagattct ttttttactg gcgaaacatc acaaagtagt gcgacataaa attcttcatt
     4321 atatcgagca gaaatcgtag cggagcgaat cgatccttca actggccgat gctgatttat
     4381 cttgattagt tctttttgtt ttggtaactt taagtagcca ttttccagat agatcgtacg
     4441 ggtttggttg ttggtcgtat aagattgcca catacttttt ttcgttttaa gctttgggaa
     4501 gctggcacgt cccttgaaat aattgcgaaa agcccgatcc aaattaagct gtgcatttgc
     4561 taaagcaaga ctgtctactt cttttaagaa tgggtattct ttttttaatg tagcgggtgt
     4621 gagcgaaaaa ttggctactc ctgtttgttg ccaacttttc tgccgctttt tcagcaaatg
     4681 gttataggta aatcgagcac atccaaaagt acgaatcaat tgctgttttt gttcttctgt
     4741 cggatacata cggaatttaa aagcttttag catctatgtc cctcctagtt agcaaccaga
     4801 agagattcct tttgtcgttt tagttttttt gcgtaatatg ggacttcctg ctcagttgca
     4861 tagacaaaat gttctggtgc gatttcacga agttgacgta cttgtccgtc atctggttca
     4921 ggttgatatt tgattcttct gcgttttctc tcatggtctg gatctggtag aggaatagca
     4981 gataacaaac tttctgtata aggatgaaca ccgtgattat agatttcatc acttgttccc
     5041 atctctaata acaagccgtt atgcattaca ccgattcggt cactaatatg tttaaccatc
     5101 gacaaatcat gtgcaataaa tagataggtc aattgatgtt ctttttggag atcttgcaac
     5161 aaattgacca cttgggcttg gatcgacaca tctagtgcag aaattggttc atcacaaatg
     5221 ataaaacgcg gttttactgc caatgctcgt gcaataccaa tacgctggcg ttgcccacct
     5281 gaaaattcat gaggataacg agtaccgtgg cttggattca atccaacagt tctcaataga
     5341 tcatcgactt tttttgcacg ctcttcagat gatttagcta gtccattaac atcaattcct
     5401 tcggcaatga tatctctgac tttcatacga ggattcaacg aagcgtatgg atcttgaaag
     5461 atcatctgaa catcatgccg gaatttcgtc aaaccttttt tatctttgat cttcgttata
     5521 tcttgaccgt cgaataatat ctcaccatca gtaggttcat tcaagcgaat gatcgtacgg
     5581 ccagtagtgg attttccgct tccagattct ccaactaaac cgaatgtttc tccttcataa
     5641 atgtgaaagc tgatgtcatt tacagcatgt acttcattct tttttcctac attgaaatac
     5701 tgtttcaatc ctttaacttc aagtagttta tttggcatca gcaattcctc cttgaccgta
     5761 agtatgtttt tgtttctctt taaagatagc ccagcgacgt tggatttctt ctggcggtgt
     5821 cactttgggt gcctgcggag caagtagcca agttgctgcc ttgtgtgttt ttgatagttc
     5881 aaaaaatggt ggttcttgtt ctgcatcgat tttcatcgcg aattcatttc ttggatagaa
     5941 tgcgtctcct tttggcgggt tcaataaatc cggtggagaa ccaggaatag catacaatcg
     6001 atcgttcgca ctatctaatg tcggcattga accaagcagt ccccaagtat agggatgttg
     6061 cgggttgtag aaaatttctt ccgatgttcc aacttcaacg attcttcctc catacatgac
     6121 agcgacacga tcagcgacat tcgctactac accaaggtca tgagtgataa agataattga
     6181 tgtatcgatt tttgtctgaa tttctttcag cagctccaaa atctgtgctt gaatagtcac
     6241 atcaagcgct gttgtaggtt catcagcaat caggatttcc ggataacata ccagtgcaat
     6301 cgcgatcacg atccgttgac gctgacctcc tgagaactga tgcggataac tgttgatacg
     6361 tttctcagct tcgggtattc caactaattt aagcaattct aatgcagctt tttgtccctc
     6421 tttttttgaa actttattat gctttctaag ggactcagcg acttgcttcc caatgggcat
     6481 cgtcggatct aaagaggtca ttggatcttg gaaaatcatc gctatttctt taccacggat
     6541 tttttgcatc tctctttctg attttccaac gatatcgtta cctttaaaga ggatttgacc
     6601 agcatctata ttggcgttgc ttgacagcaa gcgcatgatg cttctggtcg tcactgattt
     6661 tccacttcca gattccccaa cgatcgccaa tgtttctcct ttattcaaat gaaaattgac
     6721 accgcgaatc gctcgtacct ttccagcata cgtatcaaaa gagatttcta agtctttcac
     6781 ttctaaaatt ttcgtcatcg tcttcactct ttcattttag gatcaaaggc atcacgcaat
     6841 ccatcagcaa gtaagttaaa gccaatcatg ataaccgata aagttactgc cggaatccat
     6901 aatagataag gcagatacag gaatgtcttg tacccttcgc tcaacatcgt tcctaatgaa
     6961 gctgacggag gcgtaagacc taaaccgata aagcttaaaa aggcttcgaa gaaaatagca
     7021 gatggaatac taaacatcat ttgaataata atgacactag agatattcgg caaaatatgt
     7081 ttgaatgcaa ttttcattct tgattcacct aatgtcaatc cagctaaaac atattcttga
     7141 tcttttaatt tcattgtttg tgcacgaacg atacgtgcca ttggtatcca gtttgtgatg
     7201 gccatagcag ccacgattga aaaaattcct ggttcaaaaa cgaccaacat caaaatcatg
     7261 acaacgaggt ttggaattcc tgataagatt tccaaaaaac gttgcatcac gttatcgaca
     7321 cgtccgccta acaaaccaga aaccaacccg taagctacac caatcgttac atctagtaat
     7381 gcagcaataa atgcgatcag caacgagatt ctcattccca taaataagcg gcttaataca
     7441 tcacgtccca aaccgtctgt tcctaaaaag aaatttacgt ttgaagggac gtttgcttga
     7501 gcatatttat caacaagttc accaccaacc attgcttttc cattcaatcc atcgatattg
     7561 attcccggaa tacgaggagg caaattgata taagccacgt tttgggcagt tggatcatga
     7621 ggagaaacaa agattgtgat cacagagatc aaaatgatga ccaatagtaa aaccattgaa
     7681 ataacggcag ctttgttttt ctttaaacgg cgccatgaat cttggataaa attcaaagaa
     7741 ggtgcagtaa tctgttcacg ttcttcttcg gtattttttt gtaatggaag aaattcatcc
     7801 gcagggatat ctgcaactgt ttcataacgc tttggtatca tttccattta tcctcggctc
     7861 ccttctgaaa cacgaattct tggatcaact agaccgtaca atagatccac gatcaaaata
     7921 acaaaaatca gcataaatga gtaaaggatc gttacagcca tgatcgtagg ataatcattt
     7981 gttgtgattg acttaacgaa ttgttccccg ataccaggaa tagcaaagat attttctacc
     8041 accagtgatc cggtcatcaa tgctacagct aacggtccta ataaagtcat caatggaatc
     8101 aaactatttc gaagaccatg tttaaaggcg atttcccaac ggctcaatcc ttttgctttt
     8161 gctaattcca cataatcact atgcagtact tccaccattt ccgtacggat aaaccgagca
     8221 gagtcagcaa gcggcgacat ggccaacgca attgttggta agattgtgta cacaaagcca
     8281 tcccattttg cgattggcaa aacttgtaat ttgattgcaa agatatattg taataaaaca
     8341 gcaaatacaa agtttggaat ggaacgacct aatatcgcta cgagtgtaga agatgtatct
     8401 gcccatgtat tttgcttcat ggcagagatc gttcctaaaa tcgtccctag gactgttcca
     8461 aagataattg cctgtaaccc taactgcaag gatggtccga ttcttccagc taacaaatgt
     8521 gcgactggct ggtttttaaa ttggaaggaa attccaaaat ctccttgaag caaattagaa
     8581 agataaatcc catattgtac gataactggt ttatccaaac cgacttgttt atttagcatc
     8641 tcgattgttt caggactcaa acgttcctga ttcgtatatg gcgtgcccgg cagcaattgc
     8701 atcaagaaaa aggtgattgt tgcaattaac caaagcgtga tgatcatgaa aaagacacgt
     8761 tttaatacat atttgatata actattcact aatgtttccc cctagctttt gacttctgac
     8821 agatgtttcg ctacattatt agataatgaa ccaaaaataa aggagcaatc aaatgaaaaa
     8881 aaaatctatc ccctacgcag tagcttttct actgattctg gttattttaa taaaaaatct
     8941 catcaaccat tcctttacac tcattcaatt atctaatgat ctattcttat ggtcattgcc
     9001 gtttctgatt atcggcggtt ttttatgggt cttttcttcg ggtttcttcg atcattttca
     9061 gcgatcggtc catcttgcac gaacaaggaa ccgaaagaaa aaaccagaat tttcctcgct
     9121 ctcgtctgcc agctacggta tgtacagctt ttggctgatc attgccggaa tactaattgc
     9181 tctttctgct attttcatgc ttttttcttt gttaggataa gtgaatatga gaacgaggtt
     9241 gcgacatttg ccgcagcctc gttttttctc atgctcacgg tattaacgat tattcttcaa
     9301 cgtatgtcca tttgtaatcc catgaagcgc ctgccgcatg gtgaacgata cctttgattt
     9361 tttcattcac taggtgacct tctgcttttt ggtacaccgg aacgacaccc atatcatcag
     9421 agatgatctt gattgctttt tggaaatcat cccaacgggc ttgttcatcg ccagcgtctg
     9481 ttgatgaagc tgctttaact gcttcgtcat attctgcatt gctccagcga ccacggttgt
     9541 atgaattacc tgtgacaaac aaatcagtaa agctgcttgg gtctgcatag tcagcagccc
     9601 atccgcctag tactgcgtca aaatcacccg aagttgagcg gtcaagacgt acagagaatg
     9661 gaacaggtgt tggtttgact gttacgccat ccaagttttc ttggattgag ttttgaacat
     9721 attcgattac ttttttcgat gaatcatcat cagaagccat caaatcaaat tctaaagaat
     9781 caacgcccaa ttcttttttc gctttttccc aatattcttt tgctttatct ttgtcataag
     9841 aaactaattt tccagcttct tttgcaaagt cttcgttcgt tgttggactt tttaccatat
     9901 ctgatgggat caatcctgta gagacaactg atccgtcacc tagtacttgt tttactaaag
     9961 cttcacgatc gatagaataa gaaatagctt ttcttaggtt cacattgttg aatggtgaat
    10021 tcttttcacg ttgattgaat tcgatatagc ttgtccgcgc ttctttaaca gaagtataag
    10081 ctggatcgtt tgcgttttgc tgtgctaact caccagacaa gatcacgtca tctgcttggc
    10141 catctttgaa taagttcaat gcagtagaag attcttttac tacactgact ttgatttcag
    10201 aaagttttac tgtatctttg tcccagtaat gatcattttt cttataagtc cattctgtat
    10261 ctgttcctgg tccatcaaaa tcttctaatg tgaatggtcc attataaaca gctttatcac
    10321 ttgcagatgc ataggcatcc ccattttctt ccactacgga ttgtttttgt gggaaaaatg
    10381 atgggaaagc aagcagatat tggaaatatg gtgtttgttt cgtcaatttg atttctaatt
    10441 catagtcacc aactgctttg atccctagtt ctgatttatc tttctttcca gctgtaatgt
    10501 cttctgcatt ttcaactggc gcatacaggt aagcatattc agaagctgta gctgggtcta
    10561 ctgttcgttg ccagccataa acgtaatccg ctgctgtaac aggatcacca tttgaccatt
    10621 ttgcgtcttc tcgtaatttg attttgtaag ttaatccgtc ttcactgact tctgccaact
    10681 cgctagctcc tgccggctgt ggtttgctgt cttcatcaag acggtaaatt ccttcataaa
    10741 cgttattcaa tgctgtaaaa ctgattgtat ctgttgcaac tgataagtca gctgtaggca
    10801 tttcttgagg cacgactaag ttaaatacgc ctcctccatc tgctgaaccg ctttttgctg
    10861 aactactgct tgtggctgaa ctgccgccat aacatccagc aagtacaagg ccgcataccg
    10921 caaccaaacc aaatgtaaat ttctttttca ttttacgtac ccctttattg gaattttcag
    10981 aataattaat attttctgaa gttgtttaag agtttaatga ataattatta aaaaatcaac
    11041 actttttttt gtctttttca taaaataatg attttttcgt gaggtttcaa ggaatttttc
    11101 agaaaaaata gctttgtaca aggttttcca tggtcttcca aaagccatta aataattttc
    11161 gttatattgt ctattattgc catacaaaga taaaaaagaa taaacaaaaa gaaactgtga
    11221 caaaagtctt gtcacagtcc cttatctga
//
LOCUS       DAXWLB010000089        11243 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXWLB010000089 DAXWLB010000000
VERSION     DAXWLB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.089"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACGE4H_001794"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACGE4H_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFF8365200.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACGE4H_001795"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACGE4H_001795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFF8365201.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="ACGE4H_001796"
     CDS             complement(2159..2896)
                     /locus_tag="ACGE4H_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFF8365202.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="ACGE4H_001797"
     CDS             complement(2987..3514)
                     /locus_tag="ACGE4H_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365203.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="ACGE4H_001798"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="ACGE4H_001798"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFF8365204.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="ACGE4H_001799"
     CDS             complement(4391..4876)
                     /locus_tag="ACGE4H_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFF8365205.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="ACGE4H_001800"
     CDS             complement(5029..5226)
                     /locus_tag="ACGE4H_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365206.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="ACGE4H_001801"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="ACGE4H_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFF8365207.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="ACGE4H_001802"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="ACGE4H_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFF8365208.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="ACGE4H_001803"
     CDS             9201..9413
                     /locus_tag="ACGE4H_001803"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFF8365209.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="ACGE4H_001804"
     CDS             9415..11094
                     /locus_tag="ACGE4H_001804"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8365210.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3258 a         2429 c         1971 g         3585 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc tcttaaattt
    11221 atttactttt tcatacaaaa aaa
//
LOCUS       DAXWLB010000090        11131 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXWLB010000090 DAXWLB010000000
VERSION     DAXWLB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.090"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..600)
                     /locus_tag="ACGE4H_001805"
     CDS             complement(<1..600)
                     /locus_tag="ACGE4H_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365211.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEV"
     gene            complement(613..2115)
                     /locus_tag="ACGE4H_001806"
     CDS             complement(613..2115)
                     /locus_tag="ACGE4H_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365212.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2663..4342
                     /locus_tag="ACGE4H_001807"
     CDS             2663..4342
                     /locus_tag="ACGE4H_001807"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFF8365213.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4469..4948
                     /locus_tag="ACGE4H_001808"
     CDS             4469..4948
                     /locus_tag="ACGE4H_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFF8365214.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5167..5847
                     /locus_tag="ACGE4H_001809"
     CDS             5167..5847
                     /locus_tag="ACGE4H_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFF8365215.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5855..7186
                     /locus_tag="ACGE4H_001810"
     CDS             5855..7186
                     /locus_tag="ACGE4H_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFF8365216.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7201..8163
                     /locus_tag="ACGE4H_001811"
     CDS             7201..8163
                     /locus_tag="ACGE4H_001811"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFF8365217.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8274..8612
                     /locus_tag="ACGE4H_001812"
     CDS             8274..8612
                     /locus_tag="ACGE4H_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFF8365218.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8691..9398
                     /locus_tag="ACGE4H_001813"
     CDS             8691..9398
                     /locus_tag="ACGE4H_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFF8365219.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9501..10982)
                     /locus_tag="ACGE4H_001814"
     CDS             complement(9501..10982)
                     /locus_tag="ACGE4H_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365220.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3530 a         1937 c         2332 g         3332 t
ORIGIN      
        1 tacttcaatc tcatgagtca cgatgataat acagcgattt tgttcatggg cgatttcttg
       61 gaaaagtttg acgatatctt gtgaagttgt ttcatctaga tttccagtag gctcatccgc
      121 tacgattagt tgatgttcac agcagatagc acgaacgata gcgactcgct gctgctgacc
      181 tcccgaaagt ttggtcacct gtttttttgc taatatttca tcaattccta cacgagccag
      241 tcgatctaaa gcaacttgtc gataattctc ttgcttggtc ttagtgattt ccattgctgc
      301 tatgacgttg tctagagccg ataaataagg aagcaagttg taagcttgga aaataatcga
      361 gacatcattt ttacgataac ttcttaaccc gattttgttc agtgaacgat cttgatagta
      421 tacctttcct tcttttggtg catctagtcc agaaataagc gaaagaaaag tcgtttttcc
      481 agaaccactt gctcctaaaa tagcgtacat ttttccagct tcgaaattca gttctacatc
      541 tttataaagc gcatcttgcg gatcttgata ccaaaatccc aattgttctg tccgtaacat
      601 caaattcctc ctttagttta ataggatttt tcttgggttt aatcttaata tccctataga
      661 agctaaaaag acagaaaaca gactgattcc aaatgctact ccggctaata gtcccaattg
      721 tgcaggtttg acattgattt ccaattcgct gacttgatca gaaacagcaa atggattatt
      781 cgtggttcct tgttgaccat cgcgttgacc gccttgttct tgtctgttac ctggcatttg
      841 tccaccgcca ggagcttgct ggttttgtgt cgtggtttct gtctgttggt caagtagctg
      901 ctgtcccacg gcattcgcga cgacgtttcc gctaagcgaa gcaactccta atgcaagaat
      961 catacaaata gccacttcgg taaacaattg gagaatgatt ttacttctgg attctcctaa
     1021 agatagcaag acgcctaact catgccgacg ctcccgaatg gtaatcataa tgatcagagt
     1081 aagtatgata ataccggcta cagccactag taaaacaaca tttttagaaa aacttgccac
     1141 gttgtttaaa ggttccaaca ttgactgata catggaatca tttgattgta agctaaatgt
     1201 atctgtgtcg atcagcttct cagcttcttt gagaaactca tccattttat ctggatcaga
     1261 tagagtgtaa atagcagaat ctatcgtgtt atcgtcagat gtcccattta gttcatttgc
     1321 aaaagtgtaa gaagtgtaaa gtgtattcga cggattcatg aagttgaatt tcatacccat
     1381 actgccgctt gtttcgctgg attcataaat tcctttgatc gtcatttcat aagtggtatc
     1441 ttcatcttca gtactcgtga ttgtgaaagt atctccgact gccaggtcat ttgtttctgc
     1501 tagagtagaa tcaatgacta cattgtttgt gcctttgtcg ctgtcatcta ttccttcacc
     1561 atccgtgatt tttgctgttc catcagaaaa attactattt tgtgcagttg ctgacacacc
     1621 actgatttga aagtctgctt gagtcatttg cggattcccg cccattcctc cagcaggttc
     1681 accgcctgca tcagtagttt ctgaactgtc ttcacttgaa tcagaactag tgatggcagt
     1741 tattccatct tttgccatag ctgaggcgga actttcaaag gaataactct ttacattatc
     1801 taattcagct attttttccg cgtccgttaa ggaaactggt gtaagagaaa aactgcctgg
     1861 atctggacgt tcttcagtgg tagaaccgct tgaagaagtt tgttcctgtt ttttaaatga
     1921 agcttctcga ttggcactta aagtcactgt tgctccaact tctttttgtg cttgattcgc
     1981 tgcttcttct gcagcgcttc gaatcgtgag gccagctagc acaaaaataa gaatggctgt
     2041 gaacacggca aacaataaca atgaacgtcc ccattttact tttgtacttt tccatgcacg
     2101 tttcataaaa ttcatatttt gcatccctcc tagtagatgc atttgataat agacaaccta
     2161 tcttaaagct agcttaaatc ttatgctgtt tgcacaccat caacatgagg ttctgtatat
     2221 ataaaaagtc actatagtta ttgcaaaaaa tgtggtaaaa tgaaaaaagt atgtttcaaa
     2281 ccgacgaaga agaaatgaaa gaggaatagc aaaagaaatc ctagatgcta cagagggatt
     2341 tttacgtgca tagttgttct aacaattgct gatactagcg atttagttta tttgttgtat
     2401 aataatgggg aacagctgaa tacttatata agtattctgg ctatagcgtt tcgcaaaaga
     2461 tccattttgt taatgaagta tatccaaaat aaaatgccca tccaactgtc agaacgcgga
     2521 tgtgctggta attttcctgc tgattctcca taagttgaga aatagtgatt ttgcatatgc
     2581 ttcattggaa tggctcatat atttagataa agaagaaaat cgacatcttt aaacttggat
     2641 gtcataagaa gaggtgaagt ttgtgagtac cattaaaatt atcccattag gcggcgttcg
     2701 tgaaaatgga aaaaacatgt atgtcgcaga agtgggcgag gatattttta ttttagattg
     2761 tgggctaaaa tatcctgaaa atgagttgtt ggggatcgac gtcgtaatcc ctgattttac
     2821 gtatttagaa gaaaacattg atcgtgtagc tggtgtcttt ttaacacatg gacatgcaga
     2881 tgcgatcggg gcacttcctt acttgttgtc aaaaatttct gtaccagtat tcgggacaga
     2941 gttgacgatc gagttagcaa aattgaatgt cagtcgtaat gaagcagcca aaggatttaa
     3001 ggatttccat gtggtagatg agcatacaga aatagacttt ggtgaaacag tagtgagctt
     3061 tttccgtacc actcatacga taccagattc agtggggatc aatctgaaaa caaaagaagg
     3121 tagtatcgtt tatacgggag attttaaatt tgaccaaacg gctatcccaa tgtatcaaac
     3181 agattatgca cgattggcgg aaatcggcaa agaaggagtc ttagccttac taagtgaatc
     3241 ggcaaatgca gaaaatcctc agcctgtagc ttctgaagcc caaatagcag atgaagtatt
     3301 tgatacgatc aaatattggg aagggcgtat catcgtcgct tgtgtagcaa gtaatttgca
     3361 acgagtgcaa caagtattgg atgcagcaga aaaagcacaa cgaaaagttg tcttgactgg
     3421 tcaagatttc gaacgaatta ttcgcacagc gatgcgttta gaaaaacttc aattaccaag
     3481 tgaagatctg cttgttacgt tgaaagacat gaaaaactat gatccagaag aactgatcat
     3541 tttagaaact ggccgaatgg gtgaacccat caagtctctg caaaaaatgg caaacggtac
     3601 acaccgtaat gtcaagattg aagaaggaga tttggtttat atcactacta cgccaagcat
     3661 cgcgatggaa accatcgtgg cgaaaacgga agatatcatt tatcgagctg gtggaacagt
     3721 gaagttgatc tctgagaata tgcgcgtttc tggccatgcc aatcctaatg acttgcaatt
     3781 aatgatcaat ttgatgaaac caacatattt tgtgccagtt caaggagagt atcgtgaatt
     3841 agctgctcat gcagatttgg ctcatgcagt agggatgcca tacaaaaata tctatattac
     3901 tggacgcgga gacgtgctgg aatataagaa caagcgtatg agtgttgcag gaactacaag
     3961 tgcagaaaat gtaatgattg atggattagg cataggtgat atcggaaaca ttgttttacg
     4021 tgatcgaaaa atcttgtcag aagacgggat tttcgtagcg gttgtcacta tcagtcggcg
     4081 agaaaagaaa atcatttcaa aacctcaaat tacttcacgg ggattcgttt acgtaaaagc
     4141 tagccgtgat ctgatgaaag aaagcagcaa tatgattgaa acaatcgttg agaaacattt
     4201 agaaagcaat gattttgagt ggagcaaatt aaagcaagac atccgagatc aattaggccg
     4261 ttacttattt gaacaaacaa aacgccgccc agtgatcttg ccagtcatta tggaagctac
     4321 tcaacgtcga aataaaaaat aaacaaaaag agtaagatga acctctctat agagtgctcg
     4381 tcttgctctt tttttgcgaa aaacaaataa tttttgaata cagcatccct cgctttataa
     4441 taaacgatag aataaaggga gtgaatcaat gatgagtcaa acaaaagtcg aacgttattt
     4501 agaagaacaa aagatcagct atcaaccact tgattttagt catgtggtag aagaatcttt
     4561 cacagaagaa ctacaaaaaa gagggattga tccagctttg atatgtaaaa ctctcgtagt
     4621 gaaaggaaac aaaactgggg tgatcatcgc attgattcct ttgaacgatc acttagatta
     4681 caaaaaaacg agacaagtga cgaaagatcg taaagttggt tttcctggaa tggattttgt
     4741 attaatgcat acaggatatc cccacggagc aaacacccct attggaataa aactcgcaca
     4801 tcctgattat tgttttttag cagacagttc tttaaaagaa aaagaagaag taattgtgtc
     4861 aagcggtgaa atcgggagaa gccttcggat aaaagtagcc gatttagaaa agatcgtcca
     4921 tcctatttac gctgatttaa ttagttaagg gacgagaaga gaagctaagc agagcagaga
     4981 cctcaaactt gagattatat ctgcttggta cttggtgcat cctgtattat ctgttacaat
     5041 tgctttgtac atactgtact atcaatccaa tgaaaatata tttagcagct atattttttc
     5101 attgatgtgc aagtgatgaa atcgttttta ataaaaaaga tacagagaaa acgcgaggtg
     5161 taaaatatga aaattacgta tcatggacat tcctgtttaa caattgaaat ggaaaatggc
     5221 caacgcgtga tgattgaccc gtttattaca ggaaatccat tgacagactt aactgcagaa
     5281 gaagtaaaga cagattggtt gctgattact catggtcata gtgaccacat aggagatatg
     5341 attcctattg caaaagaaaa tcaagccaca attatcagta ttgttgagat tgctgattat
     5401 gcgaacagca gaggagtaga ttcattcggt atgaatatcg gtggaaaaca tgcgtttcca
     5461 ttcggcgtgg taaaattcgt acatgcacag catagttcaa gctatgaagt agacggtatc
     5521 gttcagtata tgggcgagcc tagcggaatt attattcaag cagaaggaaa aacgatttat
     5581 catgcagggg atactgctta tttcagtgat ctaggattgc ttgcagaaga attcgatatt
     5641 gatgtcgctt tcttgccgat tggcgataac tatacgatgg ggccagaaga tgcagcaaag
     5701 gctgcgcaaa caattcgtgc caagaaagta gttccaatcc attacaatac tttccctgtc
     5761 attgaacaag atcccgaatc ttttgtagag ttactggaag atagtgaagg aaaaataatg
     5821 agcgtctcgg aaacgctgga agtgtaggca gaaaatggcg acaaaacatg atttgatttt
     5881 agaatatatt gaaagtttac ctgttggcaa tcgaatttct gtaagaagca tcgcaaaaga
     5941 attgaatgtc agtgaaggaa cagcgtaccg tgcaatcaaa gatgcagaaa atgtcggact
     6001 agtttcaaca attcaacgag tagggaccat ccgtatcgaa cgcaaattaa aaaaacatat
     6061 tgaacgtttg acatttggcg aggtcgtcag gatcattgaa ggggacgttt taggcggggc
     6121 ggcaggatta gacaaggtct tgaataaatt cgtcatcggc gcgatgacta aacatgccat
     6181 ggaacgctat attacgccag gatcactaat gatcgtcggc aaccgggcgg aagtccaaaa
     6241 attagcattg gaagatggcg ccgcagtatt gatcacagga ggattcaata cgactgctga
     6301 gattgcaaag ctagctgatg atcttgaaat gccgatctta cgcacctctt atgatacatt
     6361 tacagtcgca accatgatca accgagcgtt aagcgaccaa ttgatcaaaa aagatatcat
     6421 gcttgtcagt gatatctata tgccagctga aaagactcat tatttgcagc agacagatac
     6481 gatttctgat tatcagcgtt tgtcagaaga aacgcagcat tcgcgttttc cagtcgtcaa
     6541 ccgccatcat cgtttgatgg ggattgttac ggcaaaagat gttttaggga aatcgccaaa
     6601 ccagatcatc gatcgggtga tgacgaaaga accaatcagt gtaaagaaaa caatgagtat
     6661 tgcctctgtt agccatcaaa tgatctggga tgggctagaa gtcatgccgg tcgtttccga
     6721 tgacttgaca ttggaaggct tgattacccg gcaagatgta atgaaagcga tgcagcttgt
     6781 ccagcgacaa ccgcagattg cagatacgat ttcggatcag atatctggaa atatccatac
     6841 gattgatact gaccgtgaag gaaatcgttt ggaaaaccct aagttcaaat tcgtagtagc
     6901 accacaaatg gtcaatggcg ttggaaccat ttcttttggc gtacttagcg aaatcatttc
     6961 tgatgtcgca caaaaaacaa tggtgatgaa ccaaaaacga aatatcttga ttgaacaagt
     7021 gaacctacat tatttgcggc tgatccaatt ggaaagcgaa ttagacattc gtccacgtgt
     7081 attggaaatc ggcagacgtt ccgctaaact ggatatcgaa gtgtttattg aaaacaccat
     7141 cgtttccaaa gcaatcgttg tttgtcaagt aatggaacgt tcttagaaag caggagtgaa
     7201 atgacagtac agcaagaaat agtagataca ataaaaaaat acgataaaat cattatccac
     7261 aggcatttgc gccctgatcc tgatgcatta ggatcacaag taggattagc cgaactatta
     7321 cgggaaagtt tcccagaaaa agaaatacgt caagtaggtg aaacgattga agggcttcgc
     7381 tttttagcag aaatgcaaga agttgaagat gtcttctacc aaggagcgct agtcatcgtg
     7441 actgatacag ctaattcacc cagaatcagt gatcaaagat atcagttggc agaaaaactg
     7501 atcaaaatcg accatcatcc caatgatgac ccttatggag atcttgtgtg ggtaaataca
     7561 aaagcaagta gctgtagtga gatgattgca gaatttgctt tgatgtttcc tgaatcttta
     7621 aagatgaacg ttaaggctgc acggttactt tatgcaggaa tcgtcggaga tacagggcgc
     7681 tttttgtatc cctccacgac gtcccgtaca ttagagattg cttcgatatt gagaaattat
     7741 ccatttgatg caagtgcttt gaacagagag attgaacaaa tgccgatgaa agtcgctaaa
     7801 ctttccggtt atctttatca aaatatccag gtggatgaaa atggtgcagg aagaataatc
     7861 cttccgcaag aaattcttga taaatatgga atcgatgatt cagagacagc ttcgatcgtt
     7921 tcacttcctg gagtgattga cgaagtgctg gcttggggga tttttgttca gcagccttca
     7981 gggtattacc gcgtacgttt acgttctaag gggccgatca tcaatgaatt ggctaaacgc
     8041 catcatggag gaggccatcc attagcaagt ggtgcgaatg caaaagattt agcagaagta
     8101 gaggaaatct ataaagagat ccaagatata tgccaatcgt ataagagcag aaaaacagaa
     8161 tgatacttcg ttcgaaaacg tgccttttgt agtaaaaatc acaaatgtat cttttcaaga
     8221 tcttcttttt ccgttacaat agtgctcact aaagatttta aaggagaaag aagatgacat
     8281 taccaaattg cccagaatgt ggctcagagt acacatatga agatcgaggc ttgttgattt
     8341 gcccagaatg cgggaacgaa tggaacaaag aagaacaaga aactgtcata gaagacggat
     8401 tagtggtatt agatgcaaat ggaaaccgtc tgttagaggg cgatagcgtg acagtgatta
     8461 aagacttgaa agtaaaaggt gcgtcaggtg ccattaaaca aggaacaaaa gtcaagaaca
     8521 tccgtttagt agaaggcgat cataatatcg attgcaaggt agaaggattt ggaccgatga
     8581 aactgaaatc ggaatttgtg aaaaagaatt aaagaatatt caaggtcttt tcacattctt
     8641 ttcctactac taatgataaa ataaagtaaa cgaaaaggag tgaaacgaaa atgtatggtt
     8701 atggaatgta tggtttttta gatccgacat ttcttttggt cattcttgga ttagttattt
     8761 ccggaattgc gtcagcgtac gtcaatagta ctttccgaaa atacgatcaa gtgagaagca
     8821 aaaacaatgt gactggtaca caagcagcac aatatatatt acaaagtcaa cagataaata
     8881 atgtcggtgt tcaacaaatt gccggagatc tcacagataa ctacaattca ggtaataaga
     8941 tgttgagttt gtctcaagca actgcacagt ctacttcagt tgctgcgata ggtgtagcag
     9001 ctcatgaatg cggacatgcg gtacaagatg cttcgggtta tgtcccattg aagattcgtg
     9061 cagcacttgt tccagcagct aactttggtt cgatgatttc gttccccttg attatggtag
     9121 gtgtattgct tagctggaac tctacgttaa tcaatatcgg gattttcgct ttttctttag
     9181 cattactttt ccaattggtg acattaccag tcgaatttaa cgcgtctcgg cgagcaatcc
     9241 aaattttgag tgaaggcgga ctactgacgg aagaagaagt accgatggca agacacgtat
     9301 tatttgcagc agcactgact tatgttgcag cagcgctttc gacattcctg cagctgttgc
     9361 gcttgatctt attgttcggc ggcaaccgaa gagattaaaa taaagaagtt gtgaaatcag
     9421 cgctttgatc tgagctaata ccgagagtta gctgattgaa taccatttat ttggaataag
     9481 aggatgtgac aagaattttg tcacatcctc tttgttttgt tctcataacc ttttttcata
     9541 tacagttcat gaaccgcttt tattaacttg ccaaataaca ctgtattgtg cgttgtatcc
     9601 agatagaaag tctccgtttc atctgaaatt ttctcatgct gatctgtaat gatcaaatca
     9661 gcatgttctg aatatgcggt aattttaaca gcatctttgc gaaataactg ctgcaaatgg
     9721 ctttgaagat gataatttcc tgtaaactgt ttggttatct gcaaatggat gagtaactgc
     9781 tctttcgttt ctgtttgtag taagtgttgg atcaattgac taaggtattg actgatttgg
     9841 tttctttccc cattttttga aaaaattgta tcgatttttt ctcttaattt cttttcctgt
     9901 ataaaagaac tagaatgatt ggacgtggct tctggaaagc aaagtgtaga aaaactactg
     9961 ctggattcac ccaacagttc gaaaaaaaca actgccaaca caagaaaata aaaataaatt
    10021 ggtattttat tttctaaatg gttgagagga aactgttgag ttaagtaagt catcaattga
    10081 ccgacttgtc gacaaataag gtttttgctt gtggaaaata aaaaaccaat ctcctgtttt
    10141 tgtcgaaaag agatttgatt tggaaaaaaa caatagaaaa aataattaaa gtaggctctc
    10201 tctgtttctg tctcttcaca agggcgactc actgaaaaag aagtaaagta ttctgaataa
    10261 tcatgtgtat gctgcaataa gtccatcaat tcagtgattt cagaagaagt ttttggaaaa
    10321 tatgcctgtt gtttgtttcg taatatgaat aaagttaaca gcaagtagat atttttttct
    10381 tgttttgaaa ttttttctat ttgctcatct gtactgttca tttttagtgc atttaattca
    10441 agtgaggaaa atggccaatc gttacctcca aatgtatctg aaagaaaaaa gtaaagatag
    10501 agacggatag ataactcatt tccttcaaga gtacagcaat tttttacaga taggatccgt
    10561 aaatggaatt tttttaagga atgattgagc tgttttgtca gtttttcgag ataggagggc
    10621 gatatataaa gttgttcaag taaatatttt tttggtacag cagtttgtgg ataattgatt
    10681 agtaacatca aatagttaaa acgattggat tcttttaggt agcataattt taacttagaa
    10741 aaaacagcta actttttagt ttgatcggtt tctttggttt ggatctctcc tttatgatta
    10801 gaataaataa attcatcaat caataaaaat tgcttgagtg atacatttat ttttcggatc
    10861 agttctttga ttgtccgttt tggaatatgt aagtcattgg ataattcttg taaagaacaa
    10921 ttattttttt gaatgaccat ttgtagaaca agtaactctt tttttcttgc ttcaattagc
    10981 ataaatacct cctttttatt ctgtttttgt atatagttag acaaaaactg tgagtggaaa
    11041 gtcatttcag tctgtgttat tctcacagag tgtcgccttc ttttttatat aacgttattt
    11101 aatttttttg ttaccttgtt tattctattg t
//
LOCUS       DAXWLB010000091        11090 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXWLB010000091 DAXWLB010000000
VERSION     DAXWLB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.091"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(214..1062)
                     /locus_tag="ACGE4H_001815"
     CDS             complement(214..1062)
                     /locus_tag="ACGE4H_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8365221.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1226..2128
                     /gene="gnd"
                     /locus_tag="ACGE4H_001816"
     CDS             1226..2128
                     /gene="gnd"
                     /locus_tag="ACGE4H_001816"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFF8365222.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2130..3131
                     /locus_tag="ACGE4H_001817"
     CDS             2130..3131
                     /locus_tag="ACGE4H_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFF8365223.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3128..3412
                     /locus_tag="ACGE4H_001818"
     CDS             3128..3412
                     /locus_tag="ACGE4H_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFF8365224.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3445..4242
                     /locus_tag="ACGE4H_001819"
     CDS             3445..4242
                     /locus_tag="ACGE4H_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8365225.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4256..5089
                     /locus_tag="ACGE4H_001820"
     CDS             4256..5089
                     /locus_tag="ACGE4H_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8365226.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5107..5964
                     /locus_tag="ACGE4H_001821"
     CDS             5107..5964
                     /locus_tag="ACGE4H_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8365227.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5986..6495
                     /locus_tag="ACGE4H_001822"
     CDS             5986..6495
                     /locus_tag="ACGE4H_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8365228.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6532..6933
                     /locus_tag="ACGE4H_001823"
     CDS             6532..6933
                     /locus_tag="ACGE4H_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365229.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7045..7398
                     /locus_tag="ACGE4H_001824"
     CDS             7045..7398
                     /locus_tag="ACGE4H_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365230.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7432..7875
                     /locus_tag="ACGE4H_001825"
     CDS             7432..7875
                     /locus_tag="ACGE4H_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365231.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7892..8977
                     /gene="uxuA"
                     /locus_tag="ACGE4H_001826"
     CDS             7892..8977
                     /gene="uxuA"
                     /locus_tag="ACGE4H_001826"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8365232.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8970..9683
                     /locus_tag="ACGE4H_001827"
     CDS             8970..9683
                     /locus_tag="ACGE4H_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8365233.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9890..10039)
                     /locus_tag="ACGE4H_001828"
     CDS             complement(9890..10039)
                     /locus_tag="ACGE4H_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365234.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10435..10635
                     /locus_tag="ACGE4H_001829"
     CDS             10435..10635
                     /locus_tag="ACGE4H_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8365235.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3717 a         1740 c         2400 g         3233 t
ORIGIN      
        1 attttttact actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc
       61 aatacaacct tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc
      121 tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg
      181 ttttatggat ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg
      241 tcgaacttta gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata
      301 tagagagtcc aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc
      361 tgtttcttca gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc
      421 ttggctagta ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat
      481 tgtttctttt gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa
      541 agaagcttcc atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg
      601 caaaaattta tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata
      661 gacacgttta gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa
      721 cttttttgtg tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag
      781 ctcagtatct gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg
      841 gatcaacatg gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg
      901 aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact
      961 tactttgctt ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc
     1021 gttgtacatt gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca
     1081 taataagatt atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa
     1141 aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga
     1201 gggtataaga aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg
     1261 attgaattta gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc
     1321 aagtacagct gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga
     1381 atttctagct acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat
     1441 aaccgaaaac atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga
     1501 aggcggaaac tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga
     1561 aatcggttac ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg
     1621 cttaatgatt ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt
     1681 agcaattgaa agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat
     1741 gatccataat ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt
     1801 acaaaaaagt gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc
     1861 ggtgattcgc ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt
     1921 aagtaattat cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc
     1981 gctagaaatg gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc
     2041 acaggaagaa gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg
     2101 acatgaaata gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt
     2161 ccagcagttt cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg
     2221 ttcatttctt tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag
     2281 gattcaatat cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca
     2341 aagatgaact gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag
     2401 cagcaaaaga acaccagaca atcgtatcta tcatacctgc tttaattgaa cgaaatgctg
     2461 tagcagaaaa taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag
     2521 caagagtcaa ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta
     2581 acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc
     2641 gtaacggttt tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg
     2701 aaagctttgg tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt
     2761 gtttatgtgc gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc
     2821 agatgaaaga aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag
     2881 cgatgattaa tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag
     2941 aagaaccggg acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac
     3001 atatatcggc ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg
     3061 tcgaacagat cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata
     3121 taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta
     3181 tttatatgaa gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa
     3241 aagagtactg gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt
     3301 tttgaatgac gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc
     3361 tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt
     3421 ggcggaggaa aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc
     3481 ggagtcatcc ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat
     3541 aaggagaatg gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt
     3601 ataacagatc cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca
     3661 gatacactag caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat
     3721 gaatcatttt tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat
     3781 cattctgcta aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag
     3841 ctttcagaga ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca
     3901 agaaatgcgg cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat
     3961 cgttttctac atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt
     4021 cgctgggaaa aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct
     4081 ctagaacaaa tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt
     4141 attgattcga aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata
     4201 cagtcaatga tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga
     4261 aacattaagt gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg
     4321 ctggttaggt ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt
     4381 cgtcgggttg atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat
     4441 gatttatatg ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc
     4501 agctatcgca gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt
     4561 tgcggtgcca gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac
     4621 ttttttaggt aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg
     4681 atcaattatt ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat
     4741 tgctttatat ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt
     4801 cgttatccac attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac
     4861 tgtatacgtt atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag
     4921 tgttgttttg aaatctttag atattacgat ggttacttat gccatttttg ggattattat
     4981 cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa
     5041 caattcagca tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa
     5101 tgggaaatgg ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa
     5161 gatgtaacaa aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa
     5221 cgttacttgg cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa
     5281 gatgaagact ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta
     5341 agctggggtg gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa
     5401 gaagagtatg aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg
     5461 ggattgatgg gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat
     5521 atttttatcg caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt
     5581 ccttttatct gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct
     5641 ttcactatgg gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt
     5701 gaaatgctat cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa
     5761 gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt
     5821 gatcaaatcg tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat
     5881 acgaaaaaag gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg
     5941 ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat
     6001 ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa
     6061 agtggagtga attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga
     6121 gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag
     6181 aaagctaaat tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca
     6241 aaaacagtcc aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat
     6301 attggaggga ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat
     6361 ttaaataaat cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat
     6421 atttattttc aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag
     6481 tttgatttgt cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag
     6541 aacgagctgt ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca
     6601 aaaaagagta aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca
     6661 gatgtgacag tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga
     6721 gatatcgaaa aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta
     6781 ggaagttatt tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta
     6841 ttgtccttca ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat
     6901 cctcatcacg tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg
     6961 agctactttt tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt
     7021 cgaaatactt catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt
     7081 gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga
     7141 tcgggtatga aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg
     7201 ctacagtttt cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag
     7261 ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc
     7321 tatttgacca aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt
     7381 attgctcttt ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa
     7441 atcatgatcg ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt
     7501 gtcgggacat cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca
     7561 ttcggagaga aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta
     7621 tttgttgatc taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg
     7681 actgaagaaa agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta
     7741 gaagcaatca atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc
     7801 aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat
     7861 gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga
     7921 aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat
     7981 tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt
     8041 gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca
     8101 cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact atatacgaac
     8161 aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt
     8221 tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata
     8281 cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca
     8341 gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct
     8401 ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt
     8461 agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga
     8521 tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa
     8581 aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg
     8641 ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt
     8701 tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc
     8761 ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt
     8821 cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg
     8881 atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat
     8941 aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata
     9001 tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac
     9061 aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa
     9121 tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc
     9181 gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga
     9241 aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg
     9301 cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct
     9361 tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct
     9421 ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact
     9481 cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc
     9541 atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat
     9601 atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag
     9661 aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt
     9721 cctgcacgac tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct
     9781 tatttgtagt gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga
     9841 tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt
     9901 tttttaattc ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt
     9961 ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat
    10021 ctttgttata gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga
    10081 acgcgttgat aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt
    10141 aagaaatttt aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga
    10201 atcaatcgcg gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt
    10261 atatttcatt gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc
    10321 tattttgaaa cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa
    10381 atatttattg caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat
    10441 aacggtacag taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat
    10501 ggaaatgatg tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa
    10561 gaaggccaag cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac
    10621 attgttaaag cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt
    10681 tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat agtagtgtat aaaacaattt
    10741 tgtggagcgt gaagctttta tggtagagga aaatacgaat aaagtgatgt tgataaaaag
    10801 attcctatct tgaatatgaa tcgagatgtc ttgaaaatga atgagtttga ccaatcatta
    10861 tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa ttaatgaaaa agtaaaaaag
    10921 aatatgttct aggaagaggg cggcacatct aaatttttaa gtaacggaca gttcaaatat
    10981 caagcattga aagaggctat cctactgttt gaaggaatac gaatatataa aaacgacaaa
    11041 gaggaaaaag tagttcatta aatcccttca agtgatgaaa gaatgagatc
//
LOCUS       DAXWLB010000092        11078 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXWLB010000092 DAXWLB010000000
VERSION     DAXWLB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.092"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(51..563)
                     /locus_tag="ACGE4H_001830"
     CDS             complement(51..563)
                     /locus_tag="ACGE4H_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFF8365236.1"
                     /translation="MKNYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHVQYKTIGRIHTITQDVIQSDQYLLFGEETTNEMTRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            complement(614..1672)
                     /locus_tag="ACGE4H_001831"
     CDS             complement(614..1672)
                     /locus_tag="ACGE4H_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFF8365237.1"
                     /translation="MMSCLFLLFAIISMSSGESKHMDSAVGVTHTGEYIETLPIFSEI
                     KGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPG
                     CPYPQGSARGIGGSEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLIL
                     GRGGYAAAGITEGSPYYQGAMSIIKSYNLTEYDAFAIKHWNSLPTKKTSLIAGKSKGN
                     AKVLDRIIGRPVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVI
                     MNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMAR
                     YQRSYNQAKIRSLVRKVK"
     gene            complement(1705..2493)
                     /locus_tag="ACGE4H_001832"
     CDS             complement(1705..2493)
                     /locus_tag="ACGE4H_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8365238.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVIFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            complement(2640..4661)
                     /locus_tag="ACGE4H_001833"
     CDS             complement(2640..4661)
                     /locus_tag="ACGE4H_001833"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8365239.1"
                     /translation="MKQWYSKVSFSITRRKLVFISLVIMMGVFFFHPIFVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHSIQNIAGMNRAGTLSHGLVPFFGGLLIA
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGIFFMNTHTYLEKANEVQKEINSEM
                     LTIAKDLLPEQKGKPDAIKAIRENLFAIQIKQPWLVLQFGDSDIKTVGEERVINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGNLSVQKVYLRFGMVFLTLIVNIVISMGVLALVVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKNKGMIWTSFLQVTLWFSVSTRVNELLGWMKIGGAQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGAAGGVADQMISKSMSGQRSITTTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQQAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNVAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSCQNARKGRTKKE"
     gene            complement(4662..7199)
                     /locus_tag="ACGE4H_001834"
     CDS             complement(4662..7199)
                     /locus_tag="ACGE4H_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8365240.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSYSSHVRFFFCTNEEKIEKTIKASKELVRSEGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEESAPKEAHWMKEFRIATKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLFLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIQPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAAIAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETAPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRDIYKAVGLDESWAWLNVAQGKILGNKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALNFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEKNYEEEEYKDEILEKLVEEIEVN"
     gene            complement(7221..7580)
                     /locus_tag="ACGE4H_001835"
     CDS             complement(7221..7580)
                     /locus_tag="ACGE4H_001835"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFF8365241.1"
                     /translation="MNKIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKKSNEEFVV"
     gene            complement(7582..7800)
                     /locus_tag="ACGE4H_001836"
     CDS             complement(7582..7800)
                     /locus_tag="ACGE4H_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365242.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKVSKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            complement(8322..9302)
                     /locus_tag="ACGE4H_001837"
     CDS             complement(8322..9302)
                     /locus_tag="ACGE4H_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8365243.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            complement(9315..10190)
                     /locus_tag="ACGE4H_001838"
     CDS             complement(9315..10190)
                     /locus_tag="ACGE4H_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFF8365244.1"
                     /translation="MRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPFT
                     SFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAINV
                     GIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKVFVGIGLVIAALLIFAGQMNWIPSNG
                     TASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACTF
                     SVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYSA
                     YTMLGSQLKKVSAAN"
     gene            complement(10209..11009)
                     /locus_tag="ACGE4H_001839"
     CDS             complement(10209..11009)
                     /locus_tag="ACGE4H_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFF8365245.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
BASE COUNT         3288 a         2388 c         1676 g         3726 t
ORIGIN      
        1 cttttcgtta aactataatt tctagtaaat aaaacaacgt catttttctt ttaatatgca
       61 cttcttacat aataaatctt gattttatca atcttctgca cttcttgatc caccttgatt
      121 tgaagtaaca caaaccgagt catctcattt gttgtttctt ctccaaataa taaatattga
      181 tccgattgga ttacatcttg tgtgatagta tgaatacggc caattgtttt atattgtaca
      241 tgtggatcgt tgtcaatccc attttctttt atacaacgat cggttaaata gttttttaca
      301 gattgatttc gttcgtatat atccgtgtaa ttcacccatt ttcttccaaa attctttaac
      361 aaatattgga tttcttcaga aatattgcat tctgttttgt cttcctgatt tcttttgctt
      421 tctttacttg aactttctgc ttgtatcatc ttttcttttg atattaagga aacttctgtt
      481 ttttcttcag aaaaaatcca cccacaacta gtaagtccaa acaaacaaaa acaacttaga
      541 aagaaaaaat aataattttt cacaatcgat ccttctccct tttatactaa aaattttcat
      601 cgaaagaatt cttttatttc actttccgaa ccagacttcg aattttcgct tggttatagc
      661 tgcgttgata ccgtgccata attcgcccct gctcggcgtt ctgttctacg gtggagaaac
      721 tttgtccact atttgagaca tctgttatga tacccgtgtg gccatagatt ccaggagaca
      781 atgcaccacc tgcttgccaa ttgaccacat ctcctgcttg taagtcactg ggctttggat
      841 tcatgatcac ttgccaacca taggttgccc agttataatc ttcccctatt aacatcgcaa
      901 acatgtgtcc agaacccatt aactgtgggc cacctagttg agatacatac catgccgtac
      961 caccataaca ttccccatta tttacgggtc gtccaatgat ccgatccaaa accttcgcat
     1021 tgcctttact ttttcctgca ataagcgaag tttttttggt tggtaggcta ttccaatgct
     1081 tgatcgcaaa ggcatcatat tccgttaagt tataggactt tatgatactc attgcgcctt
     1141 gataatacgg actgccttcg gtgatacctg ccgccgcata acctccacga cctagaatca
     1201 ataaacaact tccaggatct ttattgccca cacattgatt aaagttgctt tgactcgcca
     1261 ccataaatcc ataatagtca taacaagcat tcaggttcgg aaacttcata tagaaaccac
     1321 cttcagagcc accaatccca cgtgcagaac cttgtggata gggacaacca ggaaaccacg
     1381 taatcccaaa aaagttcgta tcgtttcttc cagattgtga ctgcccataa gctgattcat
     1441 atcctaattg agaaagaatc acactcggta ataactgata ctttacagct gcaccaactg
     1501 ctgcttgggc atgttcatct gttatcggac cacgcccttt gatctctgaa aaaataggta
     1561 aggtttcgat atattctccc gtatgagtaa caccaactgc tgaatccatg tgcttcgatt
     1621 caccactaga catgctaatt atcgcaaata ataaaaacag gcaactcatc atacagatgc
     1681 cactaaataa aaaaatcttt ttcactagtg aatcgcctca attcgaaagt tcccattctc
     1741 taattgtttc atagacattt catagtacat ggtaaaccaa aggtgcgttt cttgatcttg
     1801 atacaaaata ttacaatgaa tctcatatct accctctttt tttgcgactt gcaaattcga
     1861 tagttcttca aaggctaaag gctgttgaat tggtaaaaga gttgtcccat aatattttaa
     1921 ttcttctgcg tttgctttag gataaatacg aaagaaggta tataagaatt tctctaattc
     1981 ttcctgttct tttcgagaaa tgctcgttgt ttcgaaccga gtgtctactt taatcgttgc
     2041 ttttttaaca ttactcgcta caataggcaa tttcactatg gcatatgata attcgtttcg
     2101 atatacctcc accttataca cacgctcttt gaattctttt tctatctcta ataagacatg
     2161 aaatataacc acaaatgtat gtttttttga ttcatgttcc tctattgccc aaatcgagac
     2221 atttttaact tttacattcg tttctatgtc tttatctatt tcattatcca tttgcagccc
     2281 atcagctaaa taactttcta ctgcttttga tcgatttatt tgttggtcgc tttcaagact
     2341 cactgtaaaa tacaaaaatg caaaattttt cacataactt tctattccag tcatatcttt
     2401 taattgttta tcaatgactt ttgtttcatg gattgtatgc gtatcttgag aagttaaatt
     2461 attataaact ccaaaacaaa cactaaaaac caaaaagctc cagcctacaa aaaaaaggaa
     2521 gcgccttttt cgcgctccct tatagctttc ttttttcttg tctttctttc cttttagtct
     2581 tgtttcttgt tcagagagaa tagacagttt tttcttccga ggaaacatag ccctcctcct
     2641 tattcttttt tagttctacc ttttctcgcg ttttgacaag aagtcgaagt ccaacgaacc
     2701 ggagtttcaa tctttcgatt cgtacttcgt tcatgaatcg gatgttcctg cttcgacaat
     2761 ttcttatttt tagctacatt tgaaatgtta cttctttttt ctatcgtagg aaattgttct
     2821 gttcgaccaa tcattgcact tttccgtgtt tgtaatttac cagaagtcac ttgtgttttt
     2881 cctgtttgat gcgccattga acgcatcgct tgttgttttc tctcttgttg ctctaattgc
     2941 aatgcatctt ttcgtgcttc ataaaaattc ttctgtcgct cattcgcaag gttacgcgct
     3001 cgtgtttgtc gttccgactc attcattcgc ccagagaaat aatcgtattt cattttttgt
     3061 gctttgtatt gggcattggt tgggacatac tgaattgtcc gtttccattg ttctgcccta
     3121 ctttcaatcg ttgccggtaa ttctgtcata gctgaaactt tttttcctac tcgctctata
     3181 agaggtgtag tagttatact tcgttgacca ctcatagatt tggaaatcat ttggtcagct
     3241 acacctccgg cagcaccacc aacgaaattt tcaccaattc ctcgtatcat tttttgaccc
     3301 attgtaccta acatcatgcc acgtaaaaaa cgtcccgttc gatccccagc tcgtgtttgt
     3361 gcgccaccaa ttttcatcca acctaataat tcattgacac gtgtcgagac tgaaaaccat
     3421 aacgtcacct gtaagaaact ggtccaaatc attcctttgt tcttggataa gtggtttaac
     3481 tcgtgactaa tagaaaaagt aatggttaaa atgagtaaaa tgcccatttt tgtaaacaat
     3541 aattgaaaga ctttctgtaa ggcattacct aataacgatc ctgaatttgg aaacaacgag
     3601 aaaatcatcg ctacaggtaa aaatcctata aataacaaga acaatacttg cgacgcaatt
     3661 aagatgacga ctaatgccaa tacacccata ctaatgacaa tgttgacaat gagcgttaaa
     3721 aacaccatac caaaacgcaa atacactttt tgtaccgaaa gattgccatt tttttgtttg
     3781 gttacttctt cttcaacaag cttatttcgc tgctctcctt catcataagg agattctttt
     3841 aataaattga ttacacgttc ttcaccaact gtttttatat cgctatcccc aaattgcaat
     3901 actaaccagg gttgcttgat ttgaatggca aatagatttt cacgaatggc tttaattgca
     3961 tctggttttc ctttttgttc aggtaacaga tctttggcaa tggttaacat ttcactattg
     4021 atttcttttt gtacctcatt ggctttctct aaatatgtgt gcgtattcat aaagaaaatg
     4081 ccgcaacaga taaacgtaaa taaaaatgtc actgcttgtt gaatcgcttg cgtagggtgt
     4141 tgtttcacta tcccttgata aactactgaa caacctgcta aagcaattaa caatccacca
     4201 aagaaaggga ctaatccatg actcaatgtc cctgcacggt tcattccagc aatattctga
     4261 attgaatgac ttaacgaacc aatcaactct gaaagaaaat ctaattcaaa ggcttcttgg
     4321 acaagaaacc ctaaaaagta acacagatag acatttaaca tccataaaat attggtaacg
     4381 gcaaatatcc cataaataac tgtattagaa attccttcac cccatttcca cggcaaccaa
     4441 ttccacgacg tatccacaaa aaaatctaaa ttataattat ctaaaggata tgtttcaaaa
     4501 tcatatacgg catttttgcc gcctagggtt tcttttatcc aaccagctgc ttctacgaat
     4561 attgggtgaa agaaaaaaac acccatcatt atgactagac ttatgaaaac cagttttcgg
     4621 cgagtaattg aaaatgaaac ctttgaatac cactgtttca tctaattcac ctcaatttct
     4681 tcaaccagtt tttctaatat ctcgtcttta tattcttcct cttcgtagtt tttttccatt
     4741 ggcggtcgtg tatcaaatgc atggaataaa tcttcaaacc aataatacac atacaaaatg
     4801 ccaatattac catggatgtc acaaaacaga cattctccat tttctagatt ttttacacgc
     4861 tggatatttt cttctgtgac agagattcca caaaagttta atgctttggt aatttcttcg
     4921 cgatcattcg aacgaaaaat gaatttcaac ccaattttac ttctcatctt ttcatcgcct
     4981 aaatcatctg tgttttgtgt tgaaaaatca atccctgcat tgcgagatct tccttcacga
     5041 accaatttat ttcctaaaat ttttccttgc gcaacattca accacgccca actttcatct
     5101 aagccaaccg ctttataaat atctcgattt tgtttaataa aatctagcgc atatgtagct
     5161 aaactcatca taattgctat acttaaaatt tcagaagagt tatagtcctc tggtgcagtt
     5221 tcactatctg gtaacgttaa atcctgaacc agtgcgagat tacatttggc atccaaattt
     5281 aacccttgtt gattcgtgcc atctccaaat aataggcctg caatagatag ttctttgaag
     5341 ctttcaatat gctttgccag gcgttgtgcg atcgctgctt cttgtgcata taattcctga
     5401 atcactagaa gtaatccttt tgttccctca aaatcagaaa cagtttttac tgcccgttgt
     5461 aacaaaggaa accggtcttc atcccgaata cttacacccg tgatataggt gagcacatcc
     5521 aaggctaaac tttcttgatc tttttttctt ctcatcaatt gaaatggatc aagtaaacca
     5581 atattttctt tctcactggt aatattaact gtttttaagt agggggcaaa atatggcaaa
     5641 tcttcggacc aatttgaacg ttccgatttt ggatcgatac aaaaagccaa ggcgccaaac
     5701 aacaccgacc aaaataacaa gagattttca gttaaggatt tcccaccacc tagcgaacca
     5761 attaatgctt tggcaagagt attggtaacc gttcctttta ttccttgtgc cgctaaccat
     5821 ggttgaataa acactgggat tcctgtttct gcattaaatc caatcggaat accacagcca
     5881 tctcctagtt gttgtgtcgc accaatgcca agactagcaa taaattctgc gcctacatat
     5941 tgacgataat cctcaatcac tcgccttcct gctggataac ttccaaaatg taaaaacagc
     6001 tgtacattgc acggtacttc taaatacata gaatagacat caaaaaaatt gcgaacatca
     6061 ctcacacgtt ttaacatttc atcataggtc ttccctgaca cgcgaacgtt tagtgataat
     6121 acatacaaat cttctcgcgt atcttctaat ttttgttcca actcattggc tgattggtac
     6181 gcttcgcgta cattgtttcc tacatctttt ccaaccgaca taccactttc ataaatatca
     6241 gataaatcac tctttttgcc acgtactttc gccaaggcca aacgattgtc taaaagattc
     6301 acatctaacc aagaatcgat tggaaaatcc aattccgatt gtaaaaaata ataaatctct
     6361 gattgaaatg gaaataccaa atcacgggtc atacgtgaaa gagataaata cgtgatatat
     6421 tctttttctg tcccttgttc taaaattaga taatttttct tttctgtgat tcctgcactg
     6481 gccaaattca atacatcata gtgatacgta tacgtattcg tttcttcttt aaagctattg
     6541 ggtcgataca tcacttcatc taaggattgt tctttttttc cttctaaatg ttccactaaa
     6601 aacaccatgt cttctggtgt tgtttttcgt aaccgaaagt atttactaat ccggttatac
     6661 aataaattct ccaatcgctt atatcgttga attttttcat ttgggatggt ttcaaagtct
     6721 ccaaacaaac gggcattgga tcggtgaaaa aaatcttttg tcgcaatacg aaattctttc
     6781 atccaatgag cttctttggg agcactttct tctaatacca gctcaatccc aacataccaa
     6841 caaacatttt gaatatattt tccatgtagc tcttctaaaa tgtgctgaac accttctaaa
     6901 tgttggtagg caatcgtttt taattcccct tcagaacgca ccaattcttt tgaggcttta
     6961 atcgtttttt caattttttc ttcgtttgta caaaaaaaga accgaacatg cgaagaataa
     7021 ctttgacgaa tcagacgcat gattgcttgt tggagttgat acgcctgatc ttctcccacg
     7081 aagctatatg aatacattgg gaattcataa taagcgtaca cacgatcttc ttctccaaaa
     7141 actaaattgt cttctaaata ataaaaagga atcttaggca ctagttttgg tagtcgcatc
     7201 cttttcacct ccataacgta ctacacaacg aattcctcgt ttgatttctt tttttctatt
     7261 ggctttaccc attggccatg aaaggattct tttgaacgac actgaaataa aagaaaagaa
     7321 gccaaataat tataaggctt ccttccttga aattgttttc gtgtcattaa aaaagcaaat
     7381 aaccacggac aaaaaaattg tcgagtaatc gtatgttcca taacaaatgg aaaaattcga
     7441 aaaatccccc caaaaaagcc aatcccaaac catatcgcaa atgtttgtgt tacgggacga
     7501 ggtaacgcga ccccatccca caatttataa aacactcgct ctatatacca aatctttgtc
     7561 caattgcgta ttttgttcat tttatcgccc ccatgaccgc atacgatcca aaatccaatt
     7621 gaatacgcca gacgtatctc caactaacca aacagctact gctagcacaa gaatcaatgg
     7681 aatggctttg cttactttcc gttcaaaggc aatatatgcc gcaccaccaa gcatcgccaa
     7741 cataatcaaa tacttaaaat ccccaacaaa atcagtaaac acattttgaa aaaaattcat
     7801 ttgctttttc ctccctgtta atactcattg aactaatgac gatacacaat accacagagc
     7861 aaaactgata aaaatttcac ttgatattcc tcattatgca ttttttccgt tccttgattg
     7921 ctgtttttta cgtaagaagt ataaataatt tctcacacga cgatacatta cttttacagg
     7981 aacatgcgta caataaatcc ggtgctgcgt acttttcttt gttttaggat caagtttcat
     8041 agaatagaaa tcataaaaat acttaccact ttttttatcg tatgttcctt caatcaccag
     8101 tgaaaagcta gggtggtaaa tctaataaat acaattgata cttccctcta ctagataaag
     8161 ggataaaacc aagtaccttt aaatgatctg atacagtcat aaacacgatc tatctcctcc
     8221 caaataaaac aaaaaaaggc tgtaatcgca ttttgattac agtctttttt gttctctttt
     8281 tataaatgta ctaattaaag gtttacttcc ggtcttttta tttaataaat caattttccc
     8341 aaattatttt ctttagcttt attaacgatt tctgtcgcaa ctaccacatc taatacagcc
     8401 gaaccaactg ttttgaagat tgtgatttgt tctccacttg tccgaccgat caattcttca
     8461 ttgatcagtt ggcctagttc cccatgataa ctgtctcttt gaatataccc atcttgcagc
     8521 ggtgaaatga agtccccagc ttcttttaga acaccgtcca tggtatcaaa aataacaaca
     8581 tctgcggctt tgattatttc tcgtggaatt tcacacattt ccggtgtgta agcaccgact
     8641 ccgttgatgt gcgcaccttt tttgacccac tcagcagaga atgtcgcacg tttggaagtg
     8701 gtcacacttg taatgatatc tgctccttca acgcattctt gactggtttt tgttgggcgc
     8761 atcgttacag aaaactgttg catcatttct tcagcaaatc ggctggcacg gtcaaaatca
     8821 atatcaaaaa tccgaacttc ttctaattta cgaacggtca acatcgcttc taattgcgac
     8881 tgtgcttgtc cgccggttcc gatcagtgcg ccgattttag catcttctct tgcaagaagt
     8941 tctgttgctg caccttgaac agcgccggtt cttaattgtg tcaaataggt accatccaaa
     9001 caagcggata ccatacccgt ttctggatca aggacgatca tcgttgctgg tacacttggt
     9061 aagtttttct tgatgttttc aggatacacg gaaacgattt tgacacctaa cgcaccttcg
     9121 ccaccttcta cataagctgg catatataag ctttgtccgt tgctttttgg cacatcgata
     9181 ttggtgcgaa gaggcacact tgcctttcct gctgaataca atgacaatgc ttttttgtct
     9241 gcatcgatgg cttctctcat tgagaaactt ttgataatat cttctttttt aagaaataac
     9301 atttgaatcg ctccttaatt agctgctgaa acttttttta attgactgcc caacattgta
     9361 tatgcactgt atagtaagac caaaattacg atccatttca agacataggt atctaaagat
     9421 ttgactagga atacagcgac caataccccc aatacaccga aggttgaagt gaaaagtgtg
     9481 atttttctgc tgtattcatt aaatttaatg aattgaacac taccgatcgg tactgagaat
     9541 gtacaagcac ccatcatgat aggaaaggct gccactggat tcaagcctag caagtagaca
     9601 gttaccatcg ttaacgcata agaaccaatg ccgatattgt tcaatgcgcc atagacaaag
     9661 agtaaaaatc ctgccaagat taattttccg ccatacaatt cactggctgt tccgtttgat
     9721 ggaatccagt tcatttgtcc tgcaaaaatc aataatgcag caatgaccaa tcctatgccg
     9781 acaaacactt tgatcgtttt ctctggtaac ttcacgacaa atcttggtcc taaatatgca
     9841 ccgataattt gacagataat acaaacagct agtgtcttga tccctacgtt gatggcagtg
     9901 atataagaca atgccatgac ggctaccggt acgacacatt gggtattcaa cgttccgggt
     9961 agttttttca tgctgaccca tttcaacttt ggataaagaa cggttccgat agcaaagtcg
    10021 gaaacgccaa atgttgaaag aaagaagatg ataaatgacg tgaagggcaa cagacgcata
    10081 tccccaggtt cttctttgaa attttgtttg tttttgaaaa ggtctcttag aaagtaaata
    10141 ataaaatata gattgattgc gacaattagt gtcagcaaaa tattccgaat cctaaaaatt
    10201 tcctcctact agtaaatctt tctgattccc tcaaagtcct caccatattt tgctttaagt
    10261 tcttcagaag attctgcaat cacttttagt gtatatgcat catgtttgac atattctttt
    10321 gctagattga ccatggcgtg gttttttgtc caagtgacat cggttgattg cagttcttcg
    10381 ttaatggtgc caaacacgac ttcttgctca tcggcgacga tgtttatcca tcttgctcca
    10441 aagtcagcaa gtttgtctgt ttcaaagcca tgaatataat atttttctaa gggaagatca
    10501 tattcatggg tagaactgaa taaaattagt acaaacttat caactctttt ttcagcttgc
    10561 tgcaatgtct ttagcagtga atccgtgagg ttctcatgcc aaacttgaat gagcaaactt
    10621 tcttttgcgt ttttcaccaa atgctctact ttatccagaa catactcgat gccatcaact
    10681 ttccacagca tttcttcttc atctttttct ttgatcatcc ctagattctt ggtaagaaaa
    10741 gaaagatcat ttttaacact tttttctaat ttctctaaaa actcatttgc cgagatggca
    10801 tgataaagtt taggttctga tttattaacg acaatcaaat tcttttttaa tagtttttcc
    10861 aaaatgttat atactttcga gcgtggcaca cctgagattt tacttacttc atatcctgta
    10921 agattacctt tttctaatag tgtggtatac actcttgttt ccatctctga aaaatcatat
    10981 tttttcatga tttgaataat cgtttccatt gattaatcct cactttattt ttagtagcta
    11041 ctacggtaac aactataaat tctttttgat gatctgtc
//
LOCUS       DAXWLB010000093        10941 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXWLB010000093 DAXWLB010000000
VERSION     DAXWLB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.093"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..154
                     /locus_tag="ACGE4H_001840"
     CDS             <1..154
                     /locus_tag="ACGE4H_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8365246.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(208..486)
                     /locus_tag="ACGE4H_001841"
     CDS             complement(208..486)
                     /locus_tag="ACGE4H_001841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365247.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(601..1218)
                     /locus_tag="ACGE4H_001842"
     CDS             complement(601..1218)
                     /locus_tag="ACGE4H_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365248.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1613..2437)
                     /locus_tag="ACGE4H_001843"
     CDS             complement(1613..2437)
                     /locus_tag="ACGE4H_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8365249.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2780..3196
                     /locus_tag="ACGE4H_001844"
     CDS             2780..3196
                     /locus_tag="ACGE4H_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365250.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="ACGE4H_001845"
     CDS             3193..3663
                     /locus_tag="ACGE4H_001845"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8365251.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="ACGE4H_001846"
     CDS             3677..4450
                     /locus_tag="ACGE4H_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8365252.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="ACGE4H_001847"
     CDS             4464..5276
                     /locus_tag="ACGE4H_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8365253.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="ACGE4H_001848"
     CDS             5330..6295
                     /locus_tag="ACGE4H_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8365254.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="ACGE4H_001849"
     CDS             6432..9155
                     /locus_tag="ACGE4H_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8365255.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="ACGE4H_001850"
     CDS             9168..9800
                     /locus_tag="ACGE4H_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8365256.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10008..10232
                     /locus_tag="ACGE4H_001851"
     CDS             10008..10232
                     /locus_tag="ACGE4H_001851"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFF8365257.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10334..10567
                     /gene="relB"
                     /locus_tag="ACGE4H_001852"
     CDS             10334..10567
                     /gene="relB"
                     /locus_tag="ACGE4H_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8365258.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="ACGE4H_001853"
     CDS             10557..10826
                     /locus_tag="ACGE4H_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8365259.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3626 a         1743 c         2139 g         3433 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg c
//
LOCUS       DAXWLB010000094        10800 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXWLB010000094 DAXWLB010000000
VERSION     DAXWLB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.094"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(36..1001)
                     /gene="nrdF"
                     /locus_tag="ACGE4H_001854"
     CDS             complement(36..1001)
                     /gene="nrdF"
                     /locus_tag="ACGE4H_001854"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFF8365260.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(1051..3210)
                     /gene="nrdE"
                     /locus_tag="ACGE4H_001855"
     CDS             complement(1051..3210)
                     /gene="nrdE"
                     /locus_tag="ACGE4H_001855"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFF8365261.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(3369..3593)
                     /gene="nrdH"
                     /locus_tag="ACGE4H_001856"
     CDS             complement(3369..3593)
                     /gene="nrdH"
                     /locus_tag="ACGE4H_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFF8365262.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            3994..4467
                     /locus_tag="ACGE4H_001857"
     CDS             3994..4467
                     /locus_tag="ACGE4H_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFF8365263.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            4464..6596
                     /gene="feoB"
                     /locus_tag="ACGE4H_001858"
     CDS             4464..6596
                     /gene="feoB"
                     /locus_tag="ACGE4H_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFF8365264.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            6598..6732
                     /locus_tag="ACGE4H_001859"
     CDS             6598..6732
                     /locus_tag="ACGE4H_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFF8365265.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            6826..7470
                     /locus_tag="ACGE4H_001860"
     CDS             6826..7470
                     /locus_tag="ACGE4H_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365266.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            7657..8850
                     /locus_tag="ACGE4H_001861"
     CDS             7657..8850
                     /locus_tag="ACGE4H_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFF8365267.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            8843..10570
                     /locus_tag="ACGE4H_001862"
     CDS             8843..10570
                     /locus_tag="ACGE4H_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFF8365268.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         3371 a         1932 c         2257 g         3240 t
ORIGIN      
        1 tatttttcga aaaatttttg aattaaaata aatccttatt ccaatccaat caaataatcg
       61 tcgtctttca tcgcttctac atgacctaac aaatagccgt ttccgacttg tgagaagaag
      121 tcatggttgc ttgtgcctgt tgaaatcccg ttcattacga ttgggttgac atcattggca
      181 gtatctggaa agagtggatc catccctagg ttcatcaatg ctttgttggc attatagcgt
      241 aggaatgtct tgacttcttc tgtccagccg attggatcgt ataattcttc tgtataacgt
      301 tcttcgtttt cgtaaagctc ataaagaaga ttgtacatcc agtcttttaa cgtttcttgt
      361 tctgcttcag gtaactcgtt gaatcctaat tggaatttgt aaccgatgta ggttccatgt
      421 acagattcat ctcggatgat tagtttgatg atctctgcta cgttagccaa tttgttgttt
      481 cctaaataat aaagaggagt ataaaaacct gaatagaaca agaatgtttc taaaaagacg
      541 ctggcaattt tcttttctag cggtgtaccg tgtttgtaga tttcattgat acgctcagct
      601 tttttttgca gataaggatt cgtatttgtc cagtcaaaaa tttcttcgat ttcttttttc
      661 gtattcagtg tactaaaaat agaagaataa cttttggcat gaacggattc catgaattga
      721 atattgttca aaacggcttc ttcatgggga gtgcggacat ctttacgcaa ctgatccatg
      781 ccactttcag attggacagt atctagcagt gtcaaaccgc caaatacatg tcctactgta
      841 tctttttcca attgagacaa tgtacgccaa tcatctaggt catttgaaag tgggatacgt
      901 gtatctagcc aaaattgctc tgtcagcttc tcccaagtgg atttatcaat gatatcttca
      961 atttcattcc agtttattgc ttcataatat gtttctgaca tgagaatgct cctttaatta
     1021 aaatatgaat aagcgaatct atctatggta ttagatcacg cagctttcac attgattgct
     1081 tccgatttct tctgcatcat cagtaaaggt acgtacgtaa taaatggatt tgattccttt
     1141 gtggaaagcg taatgtctta aaatattcaa atcacgtgtc gtttgcttcg ttgttgtctt
     1201 ccattcataa atgccttctg ggatttcaga acgcatgaat aaagtcaaac tcatgccttg
     1261 atcgacgtgt tgttgtgccg ttgcataaac atcgattact ttacgcatgt ccatatcata
     1321 agcagaagtg taatacggaa tcgtctcatt ggatagataa ggcgctgggt aataaatttt
     1381 accgattttc ttctcttggc gttcttcgat catgcgagtg ataggatgaa tacttgcact
     1441 cgtatcgttg atatatgaaa ttgagccatt tggtgctact gccaagcgat tttgatgata
     1501 cagtccatct ttcattactg cttgtttcaa agcattccaa tcttcagcag ttgggataaa
     1561 aatgccttca aacaatgctt tgactttttc tgattttggc tggaattctc cagttacgta
     1621 tttttcgaaa taagtgccgt cagcataagc tgatttttca aaattatgga aaacttgttt
     1681 tctttcacga gcgatattgt tgctttcaac aagtgtccaa taattcaaca gcatgaagta
     1741 aatatttgta aagtctaatg aatcctctga gccatattcc atttgttctt ttgcaagata
     1801 agtgtgtaag cccatggcac ctaatccaat cgtatgattc agcttatttc cgttttggat
     1861 cggtggtacg acatcgatat cagatgcatc tgtaataaat gtcaatgcac gagtcatcgt
     1921 acgcacagat tgaccaaagt tagggctttg catcaaattc acgatatttg tcgagcctaa
     1981 gttacatgag atatctgttc ctaatacttc atattcctgt ttgccgttaa tcacagaagg
     2041 cgtttgaact tgtaaaatct ctgaacataa attactcatg atgatcttgc catcgattgg
     2101 attctcgcgg ttcgctgtat caatattgat tacgtaagga tagccagatt cttgttgtaa
     2161 ttttgagatt tcattttcaa gatcacgcgc tttgattttt tgtttgcgaa tgtttggatt
     2221 tgcaactaga tgatcatact cagctgtaat atttacataa gagaatggga caccatattc
     2281 tttttcgaca ctatatggac taaataaata catatcttcg tttttacgtg tcaattcata
     2341 aaatttatca ggtacgatca cgccaagaga aagagttttt acgcgaatct tttcatctgc
     2401 attttctttt tttgctgaaa ggaaagcaat aatgtctgga tggaaaacgt tcaaataaac
     2461 cacgccagct ccttgacgct gacctaattg attagaatag ctgaaactat cttcgaataa
     2521 tttcatgact ggaacgacac cacttgcagc accctcatat cctttgatag gagcgccggc
     2581 ttcacgaaga tttgataaag taatccctac gccgccacca atacgggaca attgcaaagc
     2641 agagttgatt gagcgcccga tagagttcat atcgtcagtg acttgagtca agaaacaaga
     2701 caccaattcg ccccgacgtt tacgtccagc attcaagaaa gaaggtgtag ctggctgata
     2761 tcgttgatgg atcatttcgt ctgcaagagc aagtgctaat tcttcatccc cgttcgcaaa
     2821 gtaaagcgca ttgaaggcta ctcgatcttc ataagtttcc agataagcgg aaccgtcgtt
     2881 ggttttcaac gcatattgag aataaaattt gtaagcagcc ataaaagatt tgaaagaaaa
     2941 atgttgctcg tccaaaaatt gatatagctt ttcaataaat gatggtgaat acaattgaat
     3001 gaattctgct tctatatatt cttgatcgat caagtaattg attttttcca taatagatgg
     3061 aaaagtcatc gtatttggtt tgacattttc ctcaaaaaag gcaactaaag cttctttatc
     3121 cttattcaaa gggatttgac cattgactgg ccgattgatt tcattattca gcttgaaata
     3181 actgacatct tgtaaatttt taagactcat acgtacgccc aaccttcatt tcgtatttga
     3241 tgatgcttga ttgcaaatca ttgcattgtt tttaagcttt aaaaaaggat ttggaagttt
     3301 gcgacaaaaa ctggtgctgt ctcaagccct tcaaatccat gtatcacgtt ttgattcttt
     3361 tagtcatgtt agctggcaag tttacgcaac tgatcaggac ggaatccgat aactgtttct
     3421 gcatcagaag taatgacagg aacactttgg aatccttgtg ctttcaacca gtctactgct
     3481 tcaggctgtt catcgatatt gacttcttca aaagcaattt gattttctgt taaaaaccgt
     3541 ttgaccatct tacattgcat acaattattt ttagaaaaga tttttacgtt catggtgagt
     3601 gcctcctcta acacattctt attatctata atagatagta taaaactctt agtaaaaaag
     3661 tcaaacaaca aacactacat attgtgttgt ggaaactgtg gaatactaag ttttgtgttt
     3721 ttgaaaaaag agctgaaaac aagtatcgtg tgggaaaata gcaaatttaa tagagaaaaa
     3781 attgtggaaa actatgaaga aatatttttt tctcattttt atctatcaat actaaagatt
     3841 cgctcataat atcacaagta taattgacat tctcaattac cgagagtatt ctatgtgaga
     3901 atgattctca tttatttgcg taacacgtaa gaaaattaga acatataatg aatgatttta
     3961 atacatgatt tatttcacaa gagaggaatc aatatgcaga ccttagcgca ggcaaagcag
     4021 aatttcgttt atcaagtcga acgaatcgaa acagaagaaa aaacaaaacg ccatttgcaa
     4081 aatatcggga tcgttgccgg aagtaaaatc gtcgtcgtca atttatcagg caacaatgcc
     4141 attttactgg taaaaagtca tcgagtggca gtaagtcggg aaatactagg tcaaatcttt
     4201 gtcaataaaa agactgcttc ctctgaaagc tggacttctc ttgatcaatt aaaagcagga
     4261 gagactggga tcgtcgtagc aatccacgga caaggcgcag tgaagcgacg gttaatggat
     4321 atgggattga cgcgaaatgt aaaaatagtt atccgaaaac tagcccctct gggagatcca
     4381 atcgaactga cagtgcgagg gtatgaactg acgttacgaa agtcagaggc cgaactaatc
     4441 ttgatacaaa gggaggaaaa agaatgaata tcgcgttagt cggaaatcca aacagcggga
     4501 aaactagctt gttcaatctc ttgactggtt cgaatcaata tgtagggaat tggccgggcg
     4561 taacagtaga aaaaaaagaa ggatcgttga aaaaaaatcc gaacattcat attcaggacc
     4621 tacccggcat ctattctttg tctccttata caccagaaga agtgattgca agagattatt
     4681 tggttcagga acagcctgaa ctgatcatca acattattga tgctaccaat ttagaacgaa
     4741 atctttattt aactacgcag ttattggatt tagacattcc tatgatcatt ggtctgaata
     4801 tgatggatgt catcaaaaaa gaaggaaaaa aaatcaatct agaaaagctt tcctatggtc
     4861 ttggtgtacc agttttacca atcagcgtat tgaaaaagca aggaatcgaa aaactaatcg
     4921 aaaagattca ggagtctgca aaaaagcggc cttctattcc tgttccctgt acatatgatc
     4981 cacgtttaga agcagcttta catgaaattg aagatgtact aggagaacaa agcggcaaga
     5041 aacgctggta tgcaatgaaa tattttgaac gagatcagaa ggtaagagag gattacgaga
     5101 tagcagtttc tcagcaaaaa gagatcgagc aactgatcca gttaacagag aaactattgg
     5161 acgacgatag tgagacgatt ctggtcaatg aacgttatga atttattacc caactatgta
     5221 cactctgtgt tgtgtcaaat gattctttcc aattatcgat gagtgacaaa atcgatcaga
     5281 ttgcaacgaa tcgttggtta gccttgccga tcttcgcatt tgtgatgtgg ctgatctatt
     5341 atctggctat tcaaacagta ggaacaatgg ggacagattg gatcaatgac acattgtttg
     5401 gtacatggtt accagaacat gttggcagat tgttagctga atggcaagta gcgggatgga
     5461 tgcaggagct gatcttgaac ggcatcatcg caggcgtggg agcagtgtta ggatttctac
     5521 cgcaattgat tatattgttt ttatgcttga gctttctgga agactgcgga tatatggcga
     5581 ggatcgcttt tgtcatggat cgacttttca ggaaattcgg tctttcagga aaatccttca
     5641 ttcctatgct gattgctaca ggatgtggag tgccaggcgt catggccagt cgcacgattg
     5701 aaaacgagca agatcgtcgt ttgacgatca tggtcacaac cttcatgcct tgttctgcaa
     5761 agttgccgat tattgcctta gtagcagggg ccttttttcc tcatcaatct tgggtagctc
     5821 cttctgccta ttttcttggt atgactgcaa ttattttctc tggaatcagc ttgaaaaaaa
     5881 cacgtttgtt tgctggagat acagctcctt ttatcatgga attgcccgca tatcattttc
     5941 ctcaatggtc agcggtccta agacaaacct atgaccgcag caagtctttt gttaaaaaag
     6001 caggaacgat tatctttgtt tctagtatcg tgatctggtt cagctcttct tataattttt
     6061 atgctcaacc tgtttctgaa gaccaaagta tcttagcctt tttcgggcga atcctggcag
     6121 ttgtttttca gccgcttggt tggggaaact ggcaaggaac agtcgcagcg attacaggat
     6181 tggtcgctaa agaaaatatt attggcacat ttggtatttt gtttggtcat gcggaagtat
     6241 ccgaaaatgg cagagaaatc tggcaagtgc tacaacacac atatactccc gcagcagctt
     6301 attcattgtt agccttcaat ttactttgtg cgccttgttt tgcggcaatt ggggctatcc
     6361 atcgggaaat gaatgccaag aaatggactt ggatcgccat aggctatcaa tgcggtttag
     6421 cttatttagt tagttttgtg atttatcagt tgggacactt attggagggt ggacaggtag
     6481 cgttagggac gtatcttgct attttactga tgataggact cgtttatgtt ttgttccgtc
     6541 agccaaaatc caagaaccag ttttatacta tattgtcatt agaaggagag gaataggatg
     6601 gcgacattct tattaagtgt gttaatcttt ggaagtgcag gcgcaattgt ctatactcgt
     6661 ttaaaatccg gtaagaattg tgatgactgt caaacagctt gcccagtaaa aaaggaacag
     6721 atcaagcgat gatcagtcgt tgcaagaggc tattctcccc ttttttgctt attttgctag
     6781 agcatagtaa gatgagggaa agtaaaaaaa gaaagaggaa agaaaatgtc tggaaagaaa
     6841 aaaatcgtcc gcccgcgcta ccagcagatt gctgttgatt tagccgaacg tatcgttgaa
     6901 aaccgatata aagtgggtga gaagatccat gcccgctcaa cacttgccag tacgtacaat
     6961 gcttctcctg agactacccg caaggctatc aatgtgctgg tagatcttgg aatcatggaa
     7021 gtacggcatg gaagtggggc atttgttgct tcgaaggaaa aagcaaaaga tttcctattg
     7081 caatatcaag atgtacaatc catccaagat acaaaagcag aaatcatggc aagtgtggag
     7141 aagcaacaag atgagctgaa tcatttttct caattattgg atcagctggt caatcaaacg
     7201 aagcgaatac ataagatgaa tcctttactt ccgtttgaat tatctttatc agaaaaagca
     7261 atgcatctgg accgttcaat tagcgagttg aatatctggc aggcgactgg cgccacagtt
     7321 atcgcaattt ctcataatga agaactgctt gtttcacctg gcccctatgc taaatttacg
     7381 gcaggggaca tcgttcattt tattggaaat gaatatacac tacagcgtat gacaaacttt
     7441 ttttacccgg agcatcagct tgcagaatga ttgtgtgaaa tattaaaaaa atattaactt
     7501 ggctaaacct tttatttaca aagatttagc caagtttttt tcagtttgga caagcaaatt
     7561 tctttcattt ttgacaaagt gactacatga tgttaacata gtgtggtcac ttttttcaga
     7621 aagaaaaatg aatttacata aaaaaggaga atttgtttgg taaaagtaga agtaaaacat
     7681 ttaacaaaga tatttggtaa aaagacacaa gccgctttag atatgatgaa tgatcaccag
     7741 ccgaaaacgg agattttaaa gaaaaccggt gcaacggttg gtgtgtatga cgtgaatttt
     7801 gacgtaaaag aaggagaaat cttcgtgatc atggggcttt ccgggagtgg gaaatccaca
     7861 ttgatccgtc tattgaatcg tctgattgag ccaacttcgg gcagtatcta tattgatgga
     7921 gaagatgtat cgaagttaag caaagaagaa ttgcgagaag tccgtcgtca taaaatcaat
     7981 atggttttcc aaaacttcgg tctgtttcca catcgaacga tcttggaaaa tacagaatac
     8041 gggttagaag tccgtggtgt acctaaagaa gaacgacaag aaaaagcaga aaaagcatta
     8101 gaaaattcta gtttgctttc atttaaagac caatatccta gtcagttatc tggtgggatg
     8161 caacaacggg ttggcctagc cagagcttta gcaaatgatc ctgagatctt gctgatggac
     8221 gaagcgttct cggctcttga tccgctgatc cggcgagaaa tgcaagacga attactggac
     8281 ctgcaagcca atgttcaaaa aacgattatt ttcattaccc atgatttgaa tgaagcttta
     8341 cggattgggg acagaatcgc cttgatgaaa gacggcgaaa tcatgcagat cggaacaggt
     8401 gaagaaatat tgaccaatcc agcaaatgat tatgtacgtg aatttgtgga agaagtcgat
     8461 cgttccaaag tgcttacggc tcaaaacatc atggtacctg cattgacgac gaatatcgaa
     8521 tctgatggac caaacgttgc attgacaagg atgcgtaatg aagaagtcag catgctgatg
     8581 gctgttgaca gaaagagaca cctgaaaggg atcatcactg cagatcaagc gttagaagct
     8641 aggaagcaaa aacgtccatt gatcgacttc ctagatgaaa atgtgacggt gattgggaaa
     8701 gacatggtgg taagtgatat tttcaatatt atttatgact caccgactcc acttgcagtt
     8761 gtagaggatg gaaaattaaa aggtgtcgtc atccgaggta gtgtgattga agcattagca
     8821 gaaacaagcg aggtgagtga acatgaataa tctattgaac tatcaattac cagtggcaaa
     8881 ctgggtagaa aatatcacag aatggtttac gacaactttt tctggcctat tttcattttt
     8941 acaaacgatt ggacaggcag tgatgtcggg gattacaaat ttactgttag tgattccagc
     9001 tcctttgttt atcttgttat tgacggtagc agctttcttt atttctaaga aacgtccagg
     9061 acttacgttg ttcacattaa tcggtctatg gtttatttat aaccaaggtc tatggaatga
     9121 tttgatgaat actgtcacac ttgttctttt atctagtgtg atctcgatca tcattggggt
     9181 accattaggg atcttgatgg caaaaagcag taaagcccaa agtattatca aaccgatcct
     9241 tgactttatg cagacaatgc caggattcgt ttatttgatt ccagcagttg catttttcgg
     9301 tatcgggatg gttccagggg tatttgcctc agtcatcttt gctctgccac caaccgttcg
     9361 ttttacgaat ttagggattc gacaagttcc taaagaatta gtagaagctt ctgattcatt
     9421 cgggagcaca agtcgtcaaa aactattcaa actagaattg cctttagcta aaagtacgat
     9481 catggcaggg atcaatcaaa caacaatgct gtcactttca atggtcgtta ttgcctctat
     9541 gatcggtgca cccggcttag gacgcggcgt attatcagca ttgcaaagag cacaagtcgg
     9601 gaatggattt gtcaacgggg ttgcattagt tatcttagca atcatcattg accgttttac
     9661 acagcatttg aaccagccaa acaataaaaa agctgcagga gcaacaacac agaaaagtaa
     9721 aaaacgtcaa ggactgatca ttggtgctgt agtagtagtg atcttaggtg cgattggtat
     9781 agggagtttt tcatctgcca aagaaacaaa acgtatcaac ttatcttatg ttgaatggga
     9841 tacagaagtt gcttcaacaa atgtagtcgg tgaagtattg aaacaaatgg gctatgatgt
     9901 aacgatgacg ccattagata attcaatcat gtggaaatct gtttcaaatg gcgagtcaga
     9961 tgccatggtt tcagcttggc tacctaaaac tcatggttca caatatgcac aatataaaga
    10021 tcaagtcgaa gacctaggtg ccaatttgac aggagcaaaa gttggtttag cagtgccagc
    10081 ctacatggat gtaaattcga tcgatgagtt aaccgatcag gcaggtaaga aaatcatagg
    10141 aatcgagcca ggagctggtg tagttacggc tgctgaaaac acgatccaaa aatatgacaa
    10201 cttgaaagac tggaaagtag agacatcttc ttcaggagca atgaccgttg cactaggtca
    10261 agcgatcaaa aaacacgaac caattgtagt tacaggatgg acacctcatt ggatgtttgc
    10321 caaatatgat ctgaaatact tagaagatcc tgaaaacgga atgggaagcg aagagcagat
    10381 ccatacgatg gttcgaaaag gattgaaaga agaccagcca gaagcttaca aagtattaga
    10441 caatttccat tggtcagaaa aagatatgga aaaagttatg ttggaaatca acaatggaaa
    10501 agatccacag caagcagcaa aagactggat caaagagaac caagaattag tcgaaagctg
    10561 gaaaaaataa aaagacagac acgaaataaa tggagcttcc aaaattggat caaatatcca
    10621 aatttggaag ctccatgttc gtctctatct tttttgtcta taaaaaaaga gaatgcgtag
    10681 ctcatcttcg aaacgttcaa agacgccgac aaactattat gaatttatgg gtgcgagtca
    10741 aagagatcga tcgtaggtat gaaagactaa tgtgattaaa gtttattatg tacgaagtgc
//
LOCUS       DAXWLB010000095        10769 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXWLB010000095 DAXWLB010000000
VERSION     DAXWLB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.095"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            121..936
                     /gene="thiD"
                     /locus_tag="ACGE4H_001863"
     CDS             121..936
                     /gene="thiD"
                     /locus_tag="ACGE4H_001863"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFF8365269.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1265..2716)
                     /gene="cls"
                     /locus_tag="ACGE4H_001864"
     CDS             complement(1265..2716)
                     /gene="cls"
                     /locus_tag="ACGE4H_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8365270.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3057..5723
                     /locus_tag="ACGE4H_001865"
     CDS             3057..5723
                     /locus_tag="ACGE4H_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8365271.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6087..6839
                     /locus_tag="ACGE4H_001866"
     CDS             6087..6839
                     /locus_tag="ACGE4H_001866"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8365272.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6861..8015)
                     /locus_tag="ACGE4H_001867"
     CDS             complement(6861..8015)
                     /locus_tag="ACGE4H_001867"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFF8365273.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8200..10641
                     /locus_tag="ACGE4H_001868"
     CDS             8200..10641
                     /locus_tag="ACGE4H_001868"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFF8365274.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3509 a         1914 c         2311 g         3035 t
ORIGIN      
        1 aaatttgatt accaagaaaa ggtagtggta tatttactga gggatacttt gaaaaacata
       61 tgatagaaac agtaacagta ttatgaaaaa tctttttata aaaatggagg aatcgatttg
      121 atggaagatc agtatgtaca agcaatgacg atcgcaggat cagatagcga tggaagcgca
      181 gggatgcaag cagatatgca tacattcttt acaagaaatg tttacggtgt ttctgtcatg
      241 accgcatgtg ttgcaggtaa ttcttacgga atcggagcaa gtgtgacatt gccaacagat
      301 tttatcgaca aagaatttga actgattgcg aaggactttc aaatacgtgc agctaaaaca
      361 ggtatgctag cagattcaaa gttgattgag acagttgtaa aaaattacca aaaatatgat
      421 tttggaccat tagtcgtgga tcccgtaatc gtaacgaaac atggaaatct tttactagaa
      481 gaatctgctt tgcaaagctt gaaagaaaag cttgtccctt tggccgaagt actgacacct
      541 aatttttatg aagcagaaaa gctgactgga cggacattag cagctgaaga agattttgtg
      601 caagcagcaa aagaactgca agatatgggt gcaaaaaacg ttatgatcaa aggacatcat
      661 gctaaaaatg cgcagcaaga aacagtcaat gactacgtat tgttcgaatc aggaaaatca
      721 ttttggttaa atgcaccatt ttatcctacc gaccggatca acggaactgg cgattcgcta
      781 tctgcatgta ttgcagctga gttagcgaaa ggaaaaacag ttgaacaagc gatccgattg
      841 gcaaaagact atgtaaaccg tgcaattcgt gaagaaataa atgtagggca caaatatggc
      901 cccatcaatc attgggcagc tggtgaatta gagtaaaaga aagaagttgt gaaaaagttg
      961 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt
     1021 tggacttatt accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct
     1081 gccgtccagc tccgagtcac aaggaacaag ttcaaaaaac agcttctcat gtttgttgaa
     1141 tattgtgact tgtgaccgag gagttggcag gagaacaccg tttgttcgga agaagggact
     1201 gtgacaagat ttttgtcaca gtccctttaa tcttagtata tttttctcta cttgaataaa
     1261 aaaattatag gattggagaa aataatcgag aaatttgttg cttgaataat agccagttag
     1321 acatttcctt gactgtttca gttgtcatgg atgaacaatg tgtctcatct tctttgaact
     1381 gagtagcaag ttcttctaaa aactcaggat cgtaaatgac agcacttgtt tcaaaattca
     1441 atcggtagct acgaatatct tgatttgctg aacctaccat gcagatttca tcatccatta
     1501 tcaatgtttt tgcatggagg aagccgttct catagacaag gatttgtaca ttttccttga
     1561 tcagctgccg agcataatat tgtgttgctc gataaataaa aggatggtcc ggtttgttgg
     1621 gaatcatgat tttcacatct acaccagagg cagttgcgat ttttaaagca gcaatgacac
     1681 tttcatcagg aacaaggtaa ggtgtctgta tccaaacacg ttttttagca gaagtaatca
     1741 atttgataaa tgaaagcttg atttgttccc tttcgttatt cggtccgctt gaaacaagct
     1801 ggatggatgt atcagcaagc ttagattcat ctcgatctgc ttttttaaag aaataattca
     1861 aatgatagcc gacctttttt tcttcaggta ccgagacgtt ccagtctgtt aagaagcgaa
     1921 gctggagcaa agaagaagcg gcaccgaaaa tccgtatatg cgtatcgcgc caatagccga
     1981 atttttttgt tgtatttaca tattgattgg caatattgaa accaccggta taactaatct
     2041 ttccatcgat cacaacgatt tttcggtgat catggtaatt caatcggaaa cgaagtaatg
     2101 ccctttgtga ggtaacaaat gtatggacat gtccaccatt tttgattaga cgattgaaat
     2161 cttttgcttt tgtgccatga gagccaaatg catcatataa tattcgaact tccacgcctt
     2221 ctgcggcttt ttcttctaaa acatgtaaga cttgctggcc gatattatct gtcacaaatg
     2281 cgtaatattc gatatggatc gaatgtttgg ctttttttag atcttgaagc agtgaatcca
     2341 atttctcttg tccgtctgtg agaagagtga cagaattcat tcttgtcagc ggcatacgat
     2401 ttaatgaaga gaagaagtca acaaattgct gtttgtcttt atcgcccatg tcaggatcgt
     2461 aatgttcaat ggtatctcct ctaaaagact gaaagttttc taattctttc aagtcacttt
     2521 gtcggagata gaattttttc ttatccgtta atccacgtcc gaaaaataaa tataatacaa
     2581 agcccacccc aggaagggca aacaatacta agagccatgc ccaaatggct gctacatctc
     2641 ggggtttgat caaaatagcc accaaagcaa taagtgcatt taatagataa agggcggtta
     2701 taatactaga taccacaaaa atgttcctcc ttacatacaa taacattagt atagcatgat
     2761 agaagaaaga agcctaccaa ggtgaatata cttttcactc ctttcatgaa aatgtaggta
     2821 aatcgggcta aagtagttgc tttttgtagg ttaataaggt aggatattag tacagttgaa
     2881 tatctcctta ggggagtaac cggcaacgtt tgttgcgata atatcaacag taagttaacc
     2941 aaatggtttt ctggtattgt atgaaatggt gagacctaag acaaatgcgt atttgtctta
     3001 ggtctctttt ttttcagaca ggactagggc caagatcaaa aaatggggga tgaaatttgg
     3061 aaaacaagga acaagcacaa caagcggtct ataaagaaac gatcgactcg ttgatgcagc
     3121 aaacgcagag ctcaaccgat ggattgagca ccacgcaagc gaaggaaaaa ttagaaagag
     3181 atggcttgaa ccagctaaaa gaaaagccga agaagccaac ttggaaattg ttcttagaaa
     3241 ctttcaaaga tgcaatggtt atcgtattgc tgattgtcgc actgattcag atggtcatgg
     3301 gtgactacat cgaattctta gttatttttg cagtattgat gttgaattct ataatcagtg
     3361 ttgtccaaac aaaaaaagca gaaggttctt tagaagcgtt gaaaaacctt tcagcgcccg
     3421 atgctcgagt gattcgtgat ggtaaggaaa aaacgattcc ggcaaacgaa cttgtggtag
     3481 gagatatcgt gttgttagac gcaggcgatt atgtacctgc tgatggacgt ttactagatg
     3541 cgggatcatt aaaagtagat gaaggtatgc tgacaggaga gtcgacttct gcagaaaaag
     3601 aagtaacgga tatcacaagt acggtaccaa tcggagatcg tacaaatatg gtattcagtg
     3661 gaacgatcgt aacttacgga cgcggtaaat tcttagtgac ttcaactggg aataatacgg
     3721 agatcggaca agttgctggc cttttggaat caacaactga gcaagtcact ccgttacaac
     3781 gaggtctgga taaattcagt aaaaaactaa gtttggctat tttggcattg tctttattga
     3841 ttttaggtat ccaactgttt cgtatttatc ttggagaagg tacaggagat atgactgcag
     3901 atatcgtcag cgcagtgatg tttgccgtag ccgtagccgt agctgctatt ccagaagcat
     3961 tacagtctat cgtaacaatc gtgttatctt tgggaacgaa taaaatggca aaacgtcatg
     4021 ccattattcg gaagcttcca gcagttgaaa cacttggttc gacaagtatc atctgtacag
     4081 ataagactgg tacattgaca caaaacaaaa tgacagttgt tgactacttt ttaggtaatg
     4141 gcgatactgg ggatttcaca aatgaccctt caaaatggac agctgacgaa cgtcgcttga
     4201 tcgaaatcag tgttttagcg aatgatgcat cgatcagtga agacggtact aaattgggcg
     4261 accctacgga agtagcgttt atcgacttta gtgaaaaatt aaaccagcct tatcaggaaa
     4321 tccgaaacaa ctacccaaga caagcagaac ttccttttga ctcagatcga aaattaatgt
     4381 ctactggaca cacaattgat aacaaaaatt acttgtttgt taaaggcgga ccagatatcg
     4441 tcttccaacg cagtaaaaaa gtattgctaa atggtgaagt cgttccgatg acggatgaat
     4501 tgatgaaaaa attccaagag caaaatgaag ctttttcaac acgcgcatta cgtgtactag
     4561 cttttgctta ccgtccactt gataattttg atttatcatt tgaagatgaa gacgatttga
     4621 tccttgttgg tttgcttgct atgatcgatc ctccacgtgc tgaagtgact caggcggtaa
     4681 cagaagcaag aagtgcagga atcaaaacaa tcatgattac tggtgaccac aaaacaacag
     4741 ctgttgctat cgcaaaacaa atcgggattt ttgaagaagg aaacctggct ttgaccggga
     4801 cggaactaga tgaattgaca gaagatcaac taaacgaagt tttagaaaaa gtcactgttt
     4861 atgcacgtgt ttcacctgag aataaaattc gtatcgttcg tgcttggcaa aataaaggtc
     4921 atatctcagc tatgacaggt gatggtgtaa atgacgcacc agcattgaaa caagcagaca
     4981 tcggaattgc tatgggaacg ggtacagatg tggcaaaaga tgcagcagcg atggtattga
     5041 cggatgataa ctttgcttct attgtcagcg cagtagaagt aggacgtaat gtgtttgata
     5101 atatcaagaa agctatttct tatctatttg caggaaattt aggtgcgatc attgcgatca
     5161 tctttgcctt agttgctgac tggtccaatc catttacagc attacaactg ttgttcatca
     5221 atttagtcaa tgattcactg ccagcaattg cattaggtac agaaaaagct gaacctgcta
     5281 cgatgaaccg cccgccaaga gatccaaatt caggaatctt tacggacaaa acattgattt
     5341 ctgtaacgta tcgtggtgtg ctgattggga ttgctgttat ctttgctcaa tggcttggca
     5401 tgcagcattc acctgaattc ggtgtagcca tgtcattttc aacattgatt tggtgtcgga
     5461 cattgcaaac attcccagca cgttcaaaca cacaaacagc tatccaagca gggttcttct
     5521 ctaacagaag tgtcttacta gctgtagttg tatgtagcgc gctttactgc ttaacaatga
     5581 tcccaggact gaaagaagtc ttctctatcc catcagcatt tggattgaca gaagtcttta
     5641 catgtatggg aatcgcactt gtgacaattg ttttgatgga aatcactaaa ttgatcattg
     5701 tacgagtaca aggaaataaa taaaacgaat caacgaggtt gtgagcctgc cgtttaattc
     5761 tgagtcacaa ggtgcaattt caacaaacag cttctcatgt ttgttgaata ttgtgacttg
     5821 tgaccgaaga gttggcagga gaacaccgtt taaggaagaa gttgtaaaat cagcattttg
     5881 agctaatact gagggtcatc tgattggaca ctgtttattc ggaaagaggg actttgacac
     5941 gactttggtc acagtccctt tttttattta tgatagacgg aaagccaatc attttattat
     6001 tcatcaaaag agatagaata gataaaagag gagaatgatt tattccgaaa agtgaaaaca
     6061 tataacaaaa aattagagga aatcaaatga atgaaaaaga atgggatgaa tttgctgaag
     6121 aatattatga aatccagcaa gaatcgcaag taactattgc tgaagatgta aaaaattatc
     6181 tgaaaacgga gaaagtcctg cctgctgaaa cagcagcaga cgttgctgga ggatctggca
     6241 gatttctttc cttagcaaaa gaaattaatc gctatgattt gatcgatttt tcttcaggaa
     6301 tgctagaaaa tgcaaaaaaa gaagcagaaa aaaatgagtt gccaaatgtt tcctttatca
     6361 aacagtcgtt agaagaattt atccaatcaa atgagacata tgaaatggtt tttacagcag
     6421 caaatccagc attggtacat cttattcaat tagaaaaatt gatgaaaaag tcaagcaaat
     6481 ggtgcgtgat aatcagagta gtggaatcaa tcgatgatgt tttttatcct atcgaaaagc
     6541 aactaggaat gatagaagaa gatcctaata catcgccagt tgtaatggat caatttgaaa
     6601 catacttaat tgatgaagga ttcaaagtga agaaaaaaga ttttacttat tttacacagg
     6661 aatcaattac tgaagaattg atgaagctat actatgacgc gtacgcaaaa gatcctgttg
     6721 tgaccaggta cattcgcaaa ctattcagtg aaaaacgaga aatccttagt acgactaagc
     6781 tgacctatag actgctcctt tggtccgcag ataaaaaaag aacctttcac gactcgtgaa
     6841 gggttctttt tatgatttta ttatattttg tagtaacgaa tatcattttc gatctttgta
     6901 atcgaaaaag gtacatctga agtatattct gcacattctc cgtgttcagg aagcgtctca
     6961 ctgaaaatcg tttcatattc gtcaacagtg atccgtgttc gagaatctag catctcttga
     7021 tgagattgtg cgaagagata ttcttgatag ttttcttcca gataacctgt aaaaaattca
     7081 cttactgcac cagaaccgta gctgaataga ccgatgcgat ctcctggctg tagtgatttc
     7141 gaattttcca gtagagaagt tagccccagg tataatgaac cagtgtaaag attaccgatt
     7201 cgtcggctgt aacggatgct ttcttcatag cgagccataa gacgttcctg attttcttcg
     7261 tctgtttggt ctaatacgct ttgcaatgcc ttttttccca tcttagtata cggaatatgg
     7321 aaagcaatcg cctgataatc ttcgagtcct cgacccgaca attctttatg tcgattccaa
     7381 actttttgga atgattcgat atacgtagaa ttagataaag gaccatcaac aacaggaaat
     7441 tcgctataat ctggacgcca gaaatcatag atatcttctg tcagaaatac gctgtcgtct
     7501 tcaatcgata aaatacgcgg gttttgagtg atcatcatcg caacagcacc gacaccttgc
     7561 gtcacttcac caccgcttgc caagccgtaa cgagcaatat cacttgctat gactagtact
     7621 tttcgttctg gatgattttt gacatattct ttcgccatgt gcaaagctgc ggtgccaccg
     7681 tagcaagcct cttttacttc aaaagaacga gcaaaagatt ggattttcag taaatggtgg
     7741 ataattaccg cactcgcttt tgaatgatca atcccggatt ctgttccgac gatgaccatg
     7801 tcgattagct cacggtcttt ttctgttacg attttgctgg cagcatttgc tcctagtgta
     7861 atgatgtctt catttgcacg attgactgcc atttggtctt gaccaatccc tatatggtat
     7921 tttgcaggat catccccacg gctttctgcc aactctgtca tatctaaata taaattagga
     7981 ataaaaaagg aaagacgatc aatccctatt ttcatttcaa cacctcttta cttatgataa
     8041 aaaacttact ttactaaggt atcataaaaa gattaaaaga aatataaaaa agagaatatt
     8101 taaggattgt taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt
     8161 acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata
     8221 gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt
     8281 gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa
     8341 atcgatcaag taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga
     8401 cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt
     8461 tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca
     8521 gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat
     8581 cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact
     8641 gacgcatttt caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct
     8701 gtgagcagag aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct
     8761 gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg
     8821 ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt
     8881 acagtattct cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt
     8941 gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta
     9001 gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca
     9061 atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt
     9121 tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta
     9181 gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca
     9241 agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac
     9301 ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat
     9361 atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa
     9421 cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag
     9481 gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg
     9541 gaaatcccaa tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg
     9601 atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga
     9661 aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa
     9721 tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt
     9781 gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact
     9841 cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa
     9901 gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg
     9961 gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca
    10021 ttggcttctg cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt
    10081 gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt
    10141 gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga
    10201 aatgatacac gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac
    10261 caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct
    10321 ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta
    10381 gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta
    10441 gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg
    10501 ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa
    10561 gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg
    10621 gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag
    10681 ttattttatt tactgcagat cttgcttttt tctctatcag aaaatatgtt tattattaga
    10741 attttttata gttaaggtta ggcatctta
//
LOCUS       DAXWLB010000096        10617 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXWLB010000096 DAXWLB010000000
VERSION     DAXWLB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.096"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(78..2198)
                     /locus_tag="ACGE4H_001869"
     CDS             complement(78..2198)
                     /locus_tag="ACGE4H_001869"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8365275.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACGE4H_001870"
     CDS             2428..3606
                     /locus_tag="ACGE4H_001870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFF8365276.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE4H_001871"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACGE4H_001871"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFF8365277.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE4H_001872"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACGE4H_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFF8365278.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACGE4H_001873"
     CDS             complement(4959..6212)
                     /locus_tag="ACGE4H_001873"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8365279.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE4H_001874"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACGE4H_001874"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFF8365280.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACGE4H_001875"
     CDS             7789..9135
                     /locus_tag="ACGE4H_001875"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFF8365281.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACGE4H_001876"
     CDS             complement(9443..9829)
                     /locus_tag="ACGE4H_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8365282.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACGE4H_001877"
     CDS             complement(10097..10246)
                     /locus_tag="ACGE4H_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365283.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3414 a         1910 c         2121 g         3172 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaa
//
LOCUS       DAXWLB010000097        10607 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXWLB010000097 DAXWLB010000000
VERSION     DAXWLB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.097"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(274..759)
                     /locus_tag="ACGE4H_001878"
     CDS             complement(274..759)
                     /locus_tag="ACGE4H_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFF8365284.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    849..1194
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1239..1880
                     /locus_tag="ACGE4H_001879"
     CDS             1239..1880
                     /locus_tag="ACGE4H_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8365285.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1899..2534
                     /locus_tag="ACGE4H_001880"
     CDS             1899..2534
                     /locus_tag="ACGE4H_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8365286.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2552..3367
                     /locus_tag="ACGE4H_001881"
     CDS             2552..3367
                     /locus_tag="ACGE4H_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8365287.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3686..5122
                     /locus_tag="ACGE4H_001882"
     CDS             3686..5122
                     /locus_tag="ACGE4H_001882"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8365288.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5311..7758
                     /locus_tag="ACGE4H_001883"
     CDS             5311..7758
                     /locus_tag="ACGE4H_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFF8365289.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7988..9055
                     /gene="rlmN"
                     /locus_tag="ACGE4H_001884"
     CDS             7988..9055
                     /gene="rlmN"
                     /locus_tag="ACGE4H_001884"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFF8365290.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9045..9251
                     /locus_tag="ACGE4H_001885"
     CDS             9045..9251
                     /locus_tag="ACGE4H_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365291.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9248..10351)
                     /locus_tag="ACGE4H_001886"
     CDS             complement(9248..10351)
                     /locus_tag="ACGE4H_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFF8365292.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3505 a         1809 c         2294 g         2999 t
ORIGIN      
        1 agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa aattccgatt
       61 tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat
      121 ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta
      181 cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg
      241 cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg
      301 ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt
      361 gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg
      421 gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc
      481 taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc
      541 agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt
      601 tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac
      661 atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt
      721 tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt
      781 tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa
      841 agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg
      901 gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc
      961 gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata
     1021 aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt
     1081 tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt
     1141 tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt
     1201 gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta
     1261 tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt accttgattg
     1321 gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac
     1381 gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga
     1441 tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac
     1501 tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac
     1561 aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa
     1621 cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg
     1681 aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa
     1741 tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg
     1801 caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa
     1861 aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga
     1921 acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga
     1981 aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg
     2041 ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat
     2101 tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc
     2161 cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa
     2221 aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat
     2281 tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag
     2341 cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg
     2401 aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa
     2461 ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt
     2521 ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt
     2581 gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg
     2641 ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat
     2701 gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt
     2761 ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt
     2821 tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag
     2881 agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc
     2941 taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa
     3001 tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa
     3061 aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag
     3121 acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa
     3181 cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg
     3241 tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga
     3301 gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag
     3361 aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac
     3421 agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg
     3481 taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa
     3541 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct
     3601 ttacatattt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg
     3661 aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga
     3721 ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg
     3781 gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac
     3841 gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa
     3901 agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg
     3961 acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat
     4021 tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca
     4081 agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga
     4141 aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa
     4201 tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa
     4261 acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt
     4321 tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc
     4381 aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa
     4441 ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga
     4501 agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa
     4561 accagatttt cttgggatca attactatca aacggcgacg aatgcattca atccattaaa
     4621 cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg
     4681 agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg
     4741 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
     4801 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
     4861 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
     4921 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
     4981 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
     5041 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
     5101 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
     5161 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
     5221 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
     5281 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
     5341 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
     5401 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
     5461 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
     5521 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
     5581 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
     5641 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
     5701 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     5761 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     5821 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     5881 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     5941 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     6001 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     6061 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     6121 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     6181 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     6241 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     6301 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     6361 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     6421 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     6481 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     6541 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     6601 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     6661 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     6721 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     6781 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     6841 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     6901 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     6961 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     7021 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     7081 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     7141 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     7201 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     7261 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     7321 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     7381 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     7441 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     7501 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     7561 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     7621 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     7681 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     7741 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     7801 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     7861 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     7921 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     7981 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     8041 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     8101 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     8161 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     8221 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     8281 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     8341 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     8401 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     8461 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     8521 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     8581 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     8641 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     8701 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     8761 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     8821 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     8881 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     8941 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     9001 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     9061 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     9121 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     9181 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     9241 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     9301 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     9361 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     9421 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     9481 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     9541 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     9601 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     9661 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     9721 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     9781 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     9841 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     9901 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     9961 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
    10021 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
    10081 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
    10141 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
    10201 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
    10261 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
    10321 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
    10381 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
    10441 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
    10501 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
    10561 gtatttctac cgattccctt tgcttttttg taattatcta aaaattc
//
LOCUS       DAXWLB010000098        10519 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXWLB010000098 DAXWLB010000000
VERSION     DAXWLB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.098"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..609)
                     /locus_tag="ACGE4H_001887"
     CDS             complement(<1..609)
                     /locus_tag="ACGE4H_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365293.1"
                     /translation="MKKKKGTQLSVDSPNWLLIITVLCFAAFIMVSPQVYLHSVILGN
                     DIMFHFNRFYETYMQIKTGNFNFFQSLYSFQSSGRIITAFYGADFAYLQGLLLIILKS
                     WFKYQLVSSFSCFFIAGASMFWLTYKCKVRTNIGLIIALLYMSSSAVSYYPIAQAFTG
                     WGAALMPLLFVPAIEALKNKERPIRPLQLAIPVTLLLSTHLLS"
     gene            complement(797..1450)
                     /locus_tag="ACGE4H_001888"
     CDS             complement(797..1450)
                     /locus_tag="ACGE4H_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365294.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSLTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1557..1766)
                     /locus_tag="ACGE4H_001889"
     CDS             complement(1557..1766)
                     /locus_tag="ACGE4H_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFF8365295.1"
                     /translation="MPNYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESLN"
     gene            complement(1843..2595)
                     /locus_tag="ACGE4H_001890"
     CDS             complement(1843..2595)
                     /locus_tag="ACGE4H_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFF8365296.1"
                     /translation="MKKNILGSVIKDARKKKKLTQEQLSKLTGYSQNTISNHENGNRS
                     LDEDNIKTYATALDLTPDDLFEALDIKNSLENKMMIIDKKQTKIDNLLDLYSQLEESR
                     QTKVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFER
                     VPNNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDY
                     ESQTIILHSINELYEDRVVSPEQIRIIGKVVF"
     gene            2737..2970
                     /locus_tag="ACGE4H_001891"
     CDS             2737..2970
                     /locus_tag="ACGE4H_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFF8365297.1"
                     /translation="MSTMEKKKNLRVEFLKPKLKLKKARLANEWTTSYVADLIGLERR
                     QYELKEKGEYPFHDYEMYILAKNFNKKVSELFF"
     gene            3019..3780
                     /locus_tag="ACGE4H_001892"
     CDS             3019..3780
                     /locus_tag="ACGE4H_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFF8365298.1"
                     /translation="MNQPQIFNFEHNEVRTFLENDVPYFVANDVAKTLGYKNPSKATN
                     DHCKKAIETWGNDSLGRRQKFKIIPESDVYRLIIKSNLPSAEKFESWVMEEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQQVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            3911..4051
                     /locus_tag="ACGE4H_001893"
     CDS             3911..4051
                     /locus_tag="ACGE4H_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365299.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            complement(4048..4218)
                     /locus_tag="ACGE4H_001894"
     CDS             complement(4048..4218)
                     /locus_tag="ACGE4H_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365300.1"
                     /translation="MNKKIAADLVVAWLNHESQISTKGESISPKEIAQAYLDIQYAVI
                     YGQLPEDRKNDD"
     gene            4298..4489
                     /locus_tag="ACGE4H_001895"
     CDS             4298..4489
                     /locus_tag="ACGE4H_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365301.1"
                     /translation="MDTQRIVLETEEKEFIETVISFVTEKGWTISNLQNAVSKVEDYM
                     KKNATLTELTIGMPAQIKD"
     gene            4563..4790
                     /locus_tag="ACGE4H_001896"
     CDS             4563..4790
                     /locus_tag="ACGE4H_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8365302.1"
                     /translation="MTQELINKNDLDSFLIGYVPKRYLTQKEAVHYTGTSAGTINEWV
                     KKGLKVIIFGENSRPKYDIKDIDEFMSKYKV"
     gene            4803..4976
                     /locus_tag="ACGE4H_001897"
     CDS             4803..4976
                     /locus_tag="ACGE4H_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365303.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(5090..5497)
                     /locus_tag="ACGE4H_001898"
     CDS             complement(5090..5497)
                     /locus_tag="ACGE4H_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365304.1"
                     /translation="MSFDNTITISIILALVALISPWITAVINNKHAESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKILPILNKSLEKL"
     gene            5659..5883
                     /locus_tag="ACGE4H_001899"
     CDS             5659..5883
                     /locus_tag="ACGE4H_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365305.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            5893..6087
                     /locus_tag="ACGE4H_001900"
     CDS             5893..6087
                     /locus_tag="ACGE4H_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365306.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDVILEYVQQHYPKIELGGI"
     gene            6090..7112
                     /locus_tag="ACGE4H_001901"
     CDS             6090..7112
                     /locus_tag="ACGE4H_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFF8365307.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGENLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKGSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDVTPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            7075..7890
                     /locus_tag="ACGE4H_001902"
     CDS             7075..7890
                     /locus_tag="ACGE4H_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFF8365308.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKTFTPIIAAVSKQTPSEARLITI
                     DQDNINYELVMLKEKIERIVRVKNGEEKPNHCGLCEYCRGNLPITGFTSMDDL"
     gene            7906..8691
                     /locus_tag="ACGE4H_001903"
     CDS             7906..8691
                     /locus_tag="ACGE4H_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365309.1"
                     /translation="MTGGWIKLYRQLLEKPIWQESTPEQKVILITLLSMANHEEREWE
                     WQGEPYKASPGQFVTSLDSIRKKCGKGISLQNVRTALKRFEKYEFLTNQPTNKNRLIT
                     IVNWEVYQSKDNDLTKELTSNQQATNKQLTTNKNDKNDKNDKNDNNPRNSRKTREYAD
                     DDPNKKLAILLLKLIRKNQNIKEPDLDKWANTIRLTIESDKRTGREVQDMIVWATSND
                     FWSGVILSPTSLRKHFDKMAIQKNKRKQQNISNDELPETGEDW"
     gene            8691..9539
                     /locus_tag="ACGE4H_001904"
     CDS             8691..9539
                     /locus_tag="ACGE4H_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8365310.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            9536..9778
                     /locus_tag="ACGE4H_001905"
     CDS             9536..9778
                     /locus_tag="ACGE4H_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365311.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            9921..10289
                     /locus_tag="ACGE4H_001906"
     CDS             9921..10289
                     /locus_tag="ACGE4H_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFF8365312.1"
                     /translation="MATRSKYGNKKHEVDGITFDSIAEAEYYDLALWQAEANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHK
                     YQVPLILAKKYRNTFKEERF"
     gene            10304..10465
                     /locus_tag="ACGE4H_001907"
     CDS             10304..10465
                     /locus_tag="ACGE4H_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8365313.1"
                     /translation="MTTEEVIQMHIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         3730 a         1747 c         2092 g         2950 t
ORIGIN      
        1 tgaaagtaaa tgagtagata gtaataaagt tacagggatt gcaagttgca atggacgaat
       61 aggtctttct ttatttttta atgcttcaat tgctggcaca aaaagaagcg gcattaaagc
      121 agcaccccaa ccagtaaaag cttgagcaat aggataatac gaaactgctg atgaagacat
      181 gtacaacaaa gcaatgatta atcctatgtt agttcgtact ttacacttat aagttagcca
      241 aaacatcgaa gcaccagcta taaaaaagca actgaaagaa gagacaagtt gatatttaaa
      301 ccatgatttt aagattatta aaagtagccc ttgcaaataa gcgaaatctg cgccataaaa
      361 agcagtaata attctgccag aactctgaaa agaatacagg gattgaaaga aattaaaatt
      421 accggttttt atctgcatat aagtttcata aaacctatta aaatgaaaca taatatcatt
      481 ccctaatata acactatgaa gatatacttg gggtgatacc attataaaag ctgcaaaaca
      541 taaaactgta ataattaata accagtttgg tgaatcaacg gataattgtg tacctttttt
      601 ctttttcatt attttactct ttcctttgtt tctttttata ttttctttct ctaataactt
      661 ttttactcct cattcctatg atattattta tatgtaggat cttagaaacg agattttagt
      721 ccgtgttgca gcacgggctt ttttctttat aactttttta gagattatag gcaaaatagt
      781 agggcataaa aatatattat tgaattccgt atttagaaaa tcctaattga acttcaccgg
      841 aagtcttttg ctgtgttgta cgcaatgctt cttcttcaga cattccattc tgtactttcc
      901 atgcaacagg cgacatcccg tatttgttaa caaaatcagt aagtgataaa gtgtcagcgt
      961 cttgctgagc gcttgtttgt tggtcttctg gattttgttg agatgctgct tgttgttctt
     1021 tctgatcttg actgataata ttgccagcat catctgtagt caatccattt tcataaaggg
     1081 ccacgccgaa agcttcccac tctttgttgg accaatttgc acgatcagct ggagttgact
     1141 gtaaagtgcg ttgtttcatc tgttcatatg tttcttcttg aggtgcggtt tggattgtac
     1201 tctgactgga gcttataact gttgggctag gctccgccgt aacttggttg gagcttgtaa
     1261 ctgttaaact agaatctgtt ttagatgtag atttgctagt agatgaactg gtttcagttg
     1321 tttcttttgt tttacttatt tttgtttctt ggttagaagt ggcatctgtt gattcagctt
     1381 ttttattatt tgaacaagct gaaagtagca gagcagtact taacaacaac ataacgctaa
     1441 cttttttcat tttatactcc tcttttcttt gatacaataa gttgtaaaga agcctactat
     1501 ataggtctaa tatctcacgt cactcaaact tggtagggga gagggcgtgt tttttgttaa
     1561 tttagacttt cgaaaactaa agtagcttgg attctatcgc caccaccgaa acctttacta
     1621 ccaccgtttg tagtggaaat agtgtgtaat cgataacctt tagaacactg tctatttata
     1681 gtgttttcaa gttctgatag gttttgcgac ccttttccta taaacttttc ttttaataca
     1741 acttgcaata caacataatt tggcatattc ttttccttct ttctactata ataataaata
     1801 ctaaccccta gccggagcgc caacttcgac tggggtattt ttttagaata cgactttccc
     1861 gattattcta atctgttccg gtgagacgac tctatcttca tataattcgt ttatagagtg
     1921 tagaataatc gtttgactct cataatcctt atatacttct ttacacgtca cgccgtcacc
     1981 atctatttca acgatagcaa tctcaccgtc ttccacgtct ggctgttcct taacaaatac
     2041 aaattgtcca ttcctgataa ctggttccat tgaatcgcct ataataggaa cagcaaaatc
     2101 agcattgttt ggaactcttt caaattcttc ttcctctaca actgtatcgc cataaactaa
     2161 ttcagtcggg ttggcggcag aagctgaatt tttgattgtc ggaagttttt cctttttagg
     2221 aaagtgcacc acgtttcctc gattttgctc ttcgagttgc tgttcagcaa agttgtagac
     2281 ttttgtttgg cgagactctt ccaattggct atacaaatct aataaattat ctattttagt
     2341 ttgtttttta tcgataatca tcattttgtt ttctagagaa tttttaatat ctaacgcctc
     2401 aaaaagatcg tccggagtta aatccaaagc cgttgcataa gttttgatat tatcttcatc
     2461 tagggatcgg tttccgtttt catggttaga tatagtattt tgactgtagc cagttaattt
     2521 actaagttgt tcttgagtaa gttttttctt ttttcttgcg tcctttataa ccgaacctaa
     2581 aatatttttc ttcattaaag gcacctcctt catgagataa caatatcaca ttgtgataaa
     2641 cgtgtaaata aaaatatctc aaaaagtgat aaaaaactat tgacatatat ctcaatatga
     2701 gatattattg atttatcaaa tgaaggaggc gattatatga gtacgatgga aaaaaagaaa
     2761 aacttaagag ttgagttttt gaagccgaag ttaaaactca aaaaagctag gcttgcaaat
     2821 gaatggacta catcttacgt agctgatttg attggtttgg aaagacgtca atatgagtta
     2881 aaagaaaaag gcgagtatcc attccatgat tatgaaatgt acattttggc aaaaaacttt
     2941 aacaaaaaag tatcagaatt atttttttaa aatcaaatat ctctatttga gataaaaaca
     3001 agaaaggaat gtaaaaaaat gaatcaacca caaattttta atttcgaaca taacgaagtt
     3061 cgaacgtttc tggaaaatga cgtcccatat tttgtagcga atgacgttgc aaaaactttg
     3121 ggatataaaa atccaagcaa agctactaat gatcattgca aaaaagcgat tgaaacatgg
     3181 ggtaacgatt cgttaggtcg tcgccaaaag ttcaaaatca ttccagaatc agatgtttat
     3241 cgcttgatta tcaaatcgaa tttaccaagc gctgagaaat tcgaatcttg ggtaatggaa
     3301 gaagtcctac ctcaaatccg aaaacacggc atgtacgcaa cagatgaact acttaacaat
     3361 ccagacttgc taatcgaagt agcaaccaaa ctcaaagaag aacgcacgtt acgattgata
     3421 gcagaacaac aagtaaatga attacagccg aaggcagact attacgaccg aatcttgaac
     3481 aacaaaggat tagttacagt tagtacgatt gctaagaact acggtatgag cgctgtatcg
     3541 tttaataaat tgctacatga attaggtatc cagttcaatc aaagtggcac atggctgcta
     3601 tacagcaagt tccaagacaa aggatacacg catattgagc catttgatta tgaagataaa
     3661 aacggcaatc ggcaagtaaa aacaaggatg aaatggacgc aaaaaggaca cattttctta
     3721 tatgaaacat tgaagaagaa taattactta ccgatgattg aacgtgaaca aacagcatag
     3781 gaggtgagat gaatggagag accaaaaggg cgcgtaacca ttgacctaaa aattacaaaa
     3841 accaactcaa aaatgagctg gcttcataga ctattctttt ggaaagatat aaaagaaagg
     3901 agagaagaat atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact
     3961 gaaacaagca aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc
     4021 agccatagca gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg
     4081 accatagata acagcatatt gaatatctaa ataagcttga gcaatttctt ttggcgaaat
     4141 tgattcgcct ttagtagata tttgtgattc gtgatttagc catgcaacaa ctaaatccgc
     4201 agcgattttt ttattcatac ttattcacca ccttatgcat tatttcagta ggccacttac
     4261 tgataagaaa attatatcaa agaaaggaat gaatagaatg gacacgcaaa ggattgtttt
     4321 agaaacagag gaaaaagaat ttattgaaac ggttattagt tttgtaaccg agaaaggctg
     4381 gacgatttct aatttgcaaa atgctgtatc aaaagtggaa gattatatga aaaaaaatgc
     4441 cacattaaca gagctgacaa ttggcatgcc agctcaaatt aaagattaat agattctgca
     4501 gaagcaaaca aaattataga ttcgattatt actatgtacg aattgtagag ggggaaaacg
     4561 aaatgacaca agaactaatc aacaaaaatg atttagattc atttcttata ggctacgttc
     4621 ctaaacgcta tctgactcaa aaagaagcgg ttcattatac aggaacgtca gcaggaacta
     4681 ttaacgaatg ggtaaaaaaa gggttgaaag taatcatctt cggtgaaaac agccgtccga
     4741 aatacgacat caaagatatc gatgaattca tgtcgaaata taaagtctaa ggaggtaagc
     4801 ggatgggaaa atttaataga gcattagtgt tcagcgcacc cctaatcgtc tatgctttag
     4861 gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt tggattttta
     4921 tggggcttga tgaagctgag tacagagcaa aaaagccaac cgagggggct gactaaagtg
     4981 tggttctgtt tattaggtgt ttatttcgtg gccgttttag taaataacta catgagaaaa
     5041 cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct tacaattttt
     5101 ctaatgattt attcaaaata ggtaatattt tgtcaatcaa ataagtttca agagattttt
     5161 gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata acctctatat
     5221 cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca gatatatttt
     5281 tatcagtgtt actaccaata caaatgccca cattgttgag aaatgttgag aaagttgttt
     5341 gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc ttcatcgatt
     5401 cagcatgctt attatttata accgctgtaa tccaagggga tataagagct actaaagcta
     5461 ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccacca gttttttacc
     5521 taaattatat caaaaaggag agaagaaaaa atgcaagaat tagtaatttt gaaaaataaa
     5581 aaaagacacg aacgccggca agcatgatcg tgtccaaaag aaaaatcttt taaaccatta
     5641 taacacagga ggaaataaat gatctcagtt aaagggttag gcgatgaaat attcgaagca
     5701 atgatgagca aagcacaaca ggacatcaaa gaaaaaatca tttctgctgc agcgcaagga
     5761 caaactagct gcactgttcg atcgagagga acgacaccaa gttttctttt ggcgctagaa
     5821 gatgaaggca ttttgaatat caaacgaaga gatggcagcg tcagattgtt ttggcaattt
     5881 taggaggccg tgatgaaaga ctttgattct ttaggtgcca gacagcaacc accaaatgaa
     5941 gctagtcctg tcggagttga ttggcaagaa aatccgttat atccaggtga tacttgctat
     6001 ctaacagaag aaggttatgt accagtagac gtaattctag agtacgtaca gcaacattat
     6061 ccaaagattg aattaggagg aatttaggaa tggcaaatga tttaacacaa acaacacaac
     6121 gttcattaga tgagcaggta atcagtaatc ttggacggtt acaagaacaa ggtttagaaa
     6181 tgccaccagg atatagtcca caaaatgcgt taaaaagtgc ttttttcgaa ctcactaaca
     6241 atacaggaga aaacttgttg cagatggctg caaacaatca agaaatgaaa acctccattt
     6301 ctaatgcact tttagacatg gttattcaag ggttatctcc agcaaaaaaa caatgctatt
     6361 tcattaaata cggaaacaag gttcagctaa tgcgttcata ttttggaaca atggcggtcc
     6421 ttgatcgtgt aactggaggg gcagatatta cgcctgtagt cgttcgacaa ggtgatgagt
     6481 ttgaagttgc aatggatgga ccaaacatgg ttgtcaaaaa gcatgaaaca aaattcgaga
     6541 atttagacaa tgaaatcatt gcagcctatg tagtcatcaa gttaacaaat ggtaaagaga
     6601 ccacaacagt catgacgaaa aagcaaatcg atcaaagttg ggctaagtca aaaatgaaag
     6661 gttctggacc gcaaaaggaa tttccagaag aaatggctaa acggacagta atcaatcggg
     6721 cagctaaaac attgatcaac acaagcaacg acaacgattt attagttcaa gctgcaaaag
     6781 atacgttaga aaatgaattt gacaatgatc ggaaagatgt aacgccacaa acagaaaaag
     6841 tggctactct cgaacaaaaa ttcttttcaa acaaaaagat tactgaacca atacaaaaag
     6901 aaccagatcc gattgtgatt ccagatgata tccaaaatga agtaactcga gttgctgatg
     6961 tacctgccca tccagaaata gagcaagcac atttgatcga aaatgaggat atcaatccaa
     7021 ttcaagaaga attgatagat attccagact ttggacgcga ggaaggtgta gaagatgtct
     7081 cagaatttga agacgacgag taccctttct gatgagaatt attactccaa tgaagctgac
     7141 tggcagtaca tgtcaacatc acaatataag tcctttttaa aatgtgaagc cgcagcctta
     7201 gcaaagttga aaggcgattg ggcaccgacg tcggatccca aagccttact cgtaggaaac
     7261 tacgtacatt cttattttga atcaaaagaa gtacatgaag cgttcaaaga agaaaataaa
     7321 tccaaaatgt tttctagtag gaagccgtat ggattgttga aagatttcca aattgctgaa
     7381 caaatgattg aacgacttaa gcaagaagaa gcattcatga acatttatca aggtgagaaa
     7441 gaaacgatcg tgactgggga actatttgga acgacatgga aaggcaagat tgattgcttg
     7501 aatgttgagg atgagtattt tgttgatatc aaaacaacca aagacatgca tgagcggaaa
     7561 tggaacgaaa actacggatc aagagaaacg ttcattgtca attttggtta cgtacttcaa
     7621 atggcaatct atcaagagct tcttttacag caatacggaa agacgtttac accgatcatt
     7681 gcagcagttt ctaaacaaac accaagtgaa gcaagactga tcacgattga tcaggacaat
     7741 ataaattatg agctggttat gttaaaagaa aaaatcgaaa gaattgtcag agtgaagaac
     7801 ggtgaagaga agccgaacca ttgcggtttg tgcgagtact gtagaggtaa tcttccaatt
     7861 actggattca ctagcatgga cgatttgtag agtggtggtg atgaaatgac tggaggatgg
     7921 ataaagcttt atcgccaatt actcgagaaa cctatttggc aagaatctac accagagcaa
     7981 aaagttattt taattaccct tctttcaatg gcgaatcatg aagaaagaga gtgggaatgg
     8041 caaggcgaac cgtacaaagc aagtccaggt caattcgtta cttctctcga tagtattaga
     8101 aaaaaatgtg gtaaaggtat ctcattgcaa aatgttagaa ctgctctaaa gaggtttgaa
     8161 aaatatgaat ttttaacaaa ccaaccaaca aacaaaaata ggcttataac cattgtcaac
     8221 tgggaggttt accaatcaaa agacaacgat ctaacaaagg aactcacaag caaccaacaa
     8281 gcaactaaca agcaactaac aactaacaag aatgataaga atgataagaa tgataagaat
     8341 gataataatc ctcgcaactc tcgaaaaaca cgagagtatg cagacgacga tccaaataaa
     8401 aaattggcca ttcttttatt aaaactcatt cgaaaaaatc aaaacatcaa ggaacctgat
     8461 ttggataaat gggcgaatac gattcgttta acaattgaat ctgacaaacg aactggtaga
     8521 gaagttcaag acatgatcgt gtgggccaca agtaatgatt tctggtcagg tgtgattttg
     8581 tcaccgacta gcttaagaaa gcatttcgat aagatggcta tccaaaaaaa taaaagaaag
     8641 caacaaaata tttctaatga tgagttacca gaaacaggtg aggattggta atgaataaaa
     8701 aactaagtgc aatggctgca ccttacggcg gattaaaaac agcagatcat aattgcccaa
     8761 aatgtggtga tccattgtat atctggaaaa caaaaaataa agatggtact gatcgatgcg
     8821 gtcctacatg tatcaataag atttgtggtt accgagaaat ggtaactaaa aatcaaaaag
     8881 aagctatcca aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga atgcttaaca
     8941 gttcaatgat tacagatgat gccatatgga ccttcgattt tgatggatac aaagtagttg
     9001 atcaggaaac agcacaaata aaagcgatgg ctcaagaatg ggccaaaaaa attgtaaatg
     9061 gtagcacgat ccatgcagtg ataaccggta gaacaggagc tggaaaaact catctaggcg
     9121 ctgcagtgat taaagaagtc atgagggcat ctaattataa aattgcttgt tcatttatca
     9181 gttatcgaga acttttagaa cagttgaaat ttgcaatgaa tgatccagaa gcaagaaaag
     9241 ccgtaacagg ttcgctgatg gctgagataa agaaatcaga tttcgtggta atcgacgatt
     9301 tgggtgcaga actaggaaga atggaagaaa acaatcaggc aacgccttat gatgttgatg
     9361 ttctcacatc gctcacagaa gctcgtctaa acaaagctac gatattcaca accaatttat
     9421 catcgaagca attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta atgaatggaa
     9481 cgaaagggaa catagctgta ttcaaaacaa cgacagacaa aaggaggaat ccagtttgac
     9541 ctttgtagtg aaaaaaatgt gctacttaga tagccgaggg cgaggagaag ctagtgtcga
     9601 gtttgctaaa caccacacga ctaaagaaga ggctgacctt gttgcaagtg tttgtggcgg
     9661 cgaagtagtc gaagttatta aacccaaaag acgattttca aaacctaaga caaagccagt
     9721 gaaaaaagag tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg caaaatgact
     9781 tgtttaaaat gcaacgatga aacagttatt tggtataaga catcgcttgg atggtcaact
     9841 tgtgaacctt gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg actcgaaaga
     9901 ctaaagaagg agtatagcaa atggcaacaa gaagcaaata cggaaacaaa aagcacgaag
     9961 tagacggcat cacttttgat tctatcgcag aagcagagta ctacgatcta gccttgtggc
    10021 aagcggaagc gaatggctgg aaagtgaaac ttcaggaacg atttgagctg atgccgaaat
    10081 ttgaactaga aggaaagaag tatcgcaaga tcgagtatat tcctgacttc acattttata
    10141 aaaacggcaa acttgtcaaa gtcgtagatg tcaaaggaat gcagacaaaa gactttaaga
    10201 tcaaggcaaa gttgttttgt cataaatatc aagttccgtt gattttagct aaaaaatatc
    10261 ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa cagaagaagt
    10321 gattcaaatg catattcgaa gccttcagcg tgagattgac gatctggaac gaacaaaggc
    10381 agtgatggtc aatgaaacgg cgagaaaggc aatcgatttg cacatagaaa atttaagaag
    10441 ggaaattcgt agattggagg aatgagcgtg gataagaaag cagcaatgaa acgaatcatt
    10501 gaactgacac attctgaga
//
LOCUS       DAXWLB010000099        10496 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXWLB010000099 DAXWLB010000000
VERSION     DAXWLB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.099"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(1..461)
                     /locus_tag="ACGE4H_001908"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACGE4H_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACGE4H_001909"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACGE4H_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFF8365314.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACGE4H_001910"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACGE4H_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFF8365315.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACGE4H_001911"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACGE4H_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFF8365316.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACGE4H_001912"
     CDS             2241..2873
                     /locus_tag="ACGE4H_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFF8365317.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACGE4H_001913"
     CDS             complement(2875..3780)
                     /locus_tag="ACGE4H_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8365318.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACGE4H_001914"
     CDS             complement(3865..4524)
                     /locus_tag="ACGE4H_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8365319.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACGE4H_001915"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACGE4H_001915"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFF8365320.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHAIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACGE4H_001916"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACGE4H_001916"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFF8365321.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACGE4H_001917"
     CDS             6767..7852
                     /locus_tag="ACGE4H_001917"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFF8365322.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACGE4H_001918"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACGE4H_001918"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFF8365323.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACGE4H_001919"
     CDS             complement(9162..9965)
                     /locus_tag="ACGE4H_001919"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFF8365324.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACGE4H_001920"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACGE4H_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACGE4H_001921"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACGE4H_001921"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3105 a         2221 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgccatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaa
//
LOCUS       DAXWLB010000100        10410 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXWLB010000100 DAXWLB010000000
VERSION     DAXWLB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.100"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            151..300
                     /locus_tag="ACGE4H_001922"
     CDS             151..300
                     /locus_tag="ACGE4H_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFF8365325.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4H_001923"
     CDS             311..1825
                     /gene="dltA"
                     /locus_tag="ACGE4H_001923"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFF8365326.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4H_001924"
     CDS             1822..3027
                     /gene="dltB"
                     /locus_tag="ACGE4H_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFF8365327.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKLFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4H_001925"
     CDS             3072..3305
                     /gene="dltC"
                     /locus_tag="ACGE4H_001925"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFF8365328.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4H_001926"
     CDS             3308..4576
                     /gene="dltD"
                     /locus_tag="ACGE4H_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFF8365329.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4717..5799
                     /locus_tag="ACGE4H_001927"
     CDS             4717..5799
                     /locus_tag="ACGE4H_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFF8365330.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5799..6602
                     /locus_tag="ACGE4H_001928"
     CDS             5799..6602
                     /locus_tag="ACGE4H_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFF8365331.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6672..7412
                     /locus_tag="ACGE4H_001929"
     CDS             6672..7412
                     /locus_tag="ACGE4H_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFF8365332.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7423..8427
                     /locus_tag="ACGE4H_001930"
     CDS             7423..8427
                     /locus_tag="ACGE4H_001930"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFF8365333.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8472..10316)
                     /locus_tag="ACGE4H_001931"
     CDS             complement(8472..10316)
                     /locus_tag="ACGE4H_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365334.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3359 a         1710 c         2100 g         3241 t
ORIGIN      
        1 aaagaaaaaa gcagtgattt gatgtgctat ttttataaaa gctatgtgac aaaactgtta
       61 gtaaaaaaac ggtttctgta agctagacac atgtttcttt ttgtcaaaat tagttacact
      121 ttgtgtgagg ttatatgagg agggaatcca atgaacaaat ggcgttctaa tccaaaattg
      181 aattattggg gaaaattcat cattcgaaca attttttata gtgcagtact gctgttcctg
      241 atttatctgt atcactataa aaatattcaa ggaggaactt ttatctataa cgaattttag
      301 gaggaagagt atggaaatca aaacgattat tgaagcaatc gatgattggg gaataaatga
      361 acctgatcga gttgcttatc aggcggatga gacgcatact tttggtgaat tgaaacaagc
      421 atcagacgca cttgcttatt atttagaaaa aaaggtagaa ggcgatggac caattgtcgt
      481 ttttggcaat ttggagtttg aaatgattgt ctcatttcta ggggttgtca aatctggcca
      541 tgcctatttg ccgattgaag agcatacacc caaggagcgt atcctatcga tctttcgggt
      601 ggctaaacca tctatggtca tcagtatcgg ggactggata gaagatattc cttcggttcc
      661 tgtcataaca aaaaaagaat ttcaagaaat caagaagata ccagtaggat tttatcccag
      721 aaatagcgta aaaggtacag aaaattttta tattattttt acttctggta ctactggaga
      781 accaaaaggc gtacaaatca gtcatgataa tctagtcagc tttgttcaat ggacgatgga
      841 agactttggg atcaagcctg gtatgcattt cttagcccaa gcgccatttt cttttgattt
      901 atcggtattt tctatttatc ctgcattagt ttctggcgga atgctgaagc cgttaaataa
      961 agcagttgtt caagactttc gtcaattatt cgcgacattg ccagccttga agttgaatgt
     1021 gtgggtttct actccatcat ttatggatat ttgcttgatg gaacctacat tcaatggaga
     1081 aaatgtgccg gaattggaca tgtttttatt ttgcggagaa gaattgacga aaaaaactgc
     1141 agaaagtcta cttgatcgat tcccaaatgc acggatatat aatacttacg gtccaacaga
     1201 agcaactgta gctatttcaa gtattcagat tgaccagcat gtactagata gttatgaccg
     1261 actaccgatt ggctacgtaa aaaaagacac gaaagtttct attgtccaag aaggtaaacc
     1321 tgtttcaaaa ggagaaacgg gtgaaatcat tattgcagga ccaagcgtat caaaaggtta
     1381 cctgaacaat cccgaaaaga cagctgctgc gttcttttgt caaaatgact tagcaagtta
     1441 ccatacaggg gatgcaggta gactagatga atatgggctt ttattttatg aaggacgtat
     1501 ggatcagcag gtcaaattac atggataccg gattgaactc ggggatatcg aacattatct
     1561 gctccaagac aaccgaatca aacaagctgt cgtcgtacca aaataccaag gaacaaaagt
     1621 acagcagctg gttgcttttg tcgtattaga aaacaaaacg aaacagccgg attttcagtt
     1681 gactaaatct atcaaagaac aattactaga agttgtaatg gattatatga ttcctcagaa
     1741 gttccattat gtcgatctgc tgcctcagac agttaatgga aaaatcgatc gaaaaaaatt
     1801 aacagctgag gtgaatcctg aatgatgtta cctcatatga tcccttatgc agagccgttt
     1861 tatttttttc ttttagccat tgcgcttgta ccgatcgttc tatctttatt gatatgggaa
     1921 aaaagattac cagtttatca atcagcagtg acacttttct ttttatttgt cagctttgga
     1981 ggtaattatt ggcaacaggg agcggcgctg attgggtatg ttttgtggca gacgatactt
     2041 gtatggggct attttgttta tcggcaaagg aaaaatcaga caggatggtt ttttgccgcc
     2101 gttattttag cgataattcc actgttttta acgaaagtga ctcctttttt tgaacatgga
     2161 aaagcatcgt tacttggttt tttaggaatc tcttatttaa cctttaaatc tgtacaaatg
     2221 atcatggaaa taagagatgg catgatcaaa acatataagc catttcgtta tatccagttt
     2281 ctgctgttct ttccaacaat ctcttctgga ccaatcgatc gctataggag atttgaaaaa
     2341 gatcagctcc atccgcctgt tcgtgataaa tatgttgatc ttttgggaaa aggcgtccaa
     2401 aatatttttg tcggtttttt atataaattc attatcggtt attattttgg tcaagtactg
     2461 cttcctgtag tgggacgcat tgccttacag catggtggta tttcatgggc attagtaggt
     2521 tatatgtacg tgtacagtat gtatttgttt tttgattttg ctggctatag cttatttgca
     2581 gtcggtacaa gctacatgat gggatatcag acaccaatca atttcaataa acttttttta
     2641 tcatggaata tcaaagaatt ctggaatcgt tggcatatga cgctgtcctt ttggttccgt
     2701 gattatgtat atatgcgatt gatgttcttc atgatgaaga aaaaattttt taaaagcaag
     2761 atcgtaatgt caaatatcgg ttattttgct ttgtttcttc taatgggtat atggcatgga
     2821 ttgacgtggt actacatcgt atatggactg tatcatgctt gtctgatctg tctgacagat
     2881 gcatggatcc gatttaagaa gaagcataaa gagcagttgc cttctaacaa atttacgcat
     2941 ggcttagccg tatttttgac atttcaagca gtatgtttta gcttcctgat tttttcagga
     3001 tttttagata aacttttttt tagctaacga atagtgacat attaatttat ataacaaagg
     3061 agaaaaggaa aatggaagaa caaattttat caatcttaga agaaataaca ggaacagatg
     3121 aagtaagaaa aaatcgtgat attgatctat ttgatgaagg gttgcttgat tctttagcat
     3181 ctgttcaatt attagtcgag ctagacggag agctggatat ccaagtccct gtatctgaat
     3241 tcgagcgaga ggattggagc acaccgaata aaatcattca acaagcaact gcattgaagg
     3301 gttgagtatg aaaaagaaac tttttgctat tttcggcccg gttgttttag ctgttggctt
     3361 actgtttgtg ttttttacct cctcctatca agtagatatc tctaatccag aaacgattag
     3421 acgtgcctct tcttccatgt cagtgaacgt gttgaaagga gatgcgataa aaaattatgc
     3481 attatctgaa aaacaatata ttcctttttt tggctcaagt gaattaagca gaatcagtcc
     3541 atttcatccg tctgttctgg cagaaaaata ccagcgaaac tatcgtccat ttttgctggg
     3601 agcacccggg acacaatcgt taagccaata tatgatgatg cgttctgcag gagatgctat
     3661 gaaaaacaag aaagtcgtct ttattatttc tcctcagtgg ttcgttaaaa atggtgtaaa
     3721 aactgattac tttaatacgt attattctga acttcaaaca tacgactggt tgttttcaat
     3781 gaagaaagta acgcctgctg atcgctattt agctagaaga ttattaactt tctcaaaagt
     3841 aaaagagaat gatacgttga cggcgattct gcaaacgatc aaaaaaggga agctaccttt
     3901 acctgaatct ttgaatcagc tgcgcagcca atggaacatg ctgaagagag aggatgaagt
     3961 attcagcaat attgggttga aagatcgaca gcaaaaaatc gatcatgagt caaaacgatt
     4021 accaaaacaa taccaagaaa cagaattatc cattttagca aatcaaattg gagaacgaga
     4081 aacgacgaat aatccattcg ggttgaaaaa cgatttttat acgcatcgta tccgagccca
     4141 cgagccagag ctcaaacagt cacaaaaaaa ttgggattat cgtttttcgc cagaattttc
     4201 tgattttcag ctggtcttgg atcaattagc aaaaaaccat aacgaagtat tgttcatcat
     4261 cccaccggtt aacgagaaat ggagcgatta taccggacta tctcaagaaa tgttacaagg
     4321 atttgccaaa aagatcaaat ttcaattaaa tagccaagga ttcaatcgaa tagctgattt
     4381 tgtcaatcaa gcgggtacca actattttat ggaagacacg atccatctag gctggaaagg
     4441 ctggttggct gcagatcaac agatcagacc gtttttagaa gaaaatcata ttactgctag
     4501 taagtatcat cttgacgatg ctttttttag caaatcttgg caacatcaaa taccagataa
     4561 actacaatta aaataaagaa aacaggcgag tgttgctcgc ttgttttctt tatttttgaa
     4621 tattctgaat aaaagataaa aataccaagt ttgcctgaac aaaaatcata agcaatggta
     4681 taatcacaat atgaatatag aaatggggga aaacatttgg atacaaaaga agaacagcta
     4741 cttattgatt taacttctgc aaaaggagta cctggaaatg aagaagaggt aagagaagta
     4801 ttcagagaat atgccaaacc attcgctgat gatatttttt atgacggttt aggcagtgtg
     4861 attgccaaac atggtgctgg cggaggcccc aaggtcttta tttctggaca tatggacgaa
     4921 gttggtttta tggtaacaaa aataaccgaa aaaggattct tggaattcca aactcttgga
     4981 ggttggtggg ggcaagtcat gcttgctcag caagttgaaa tcaaaacacg tgaagggaaa
     5041 atcgtccacg gtgtgattgg ttccaaacca ccacatgttt taacacctca agtaagaaat
     5101 aaaccttatg aaataaagga tatgttcatt gatattggcg catcaagcca agaagaagcc
     5161 aaagagtggg gcatacgtcc tggagatatg gtcactccat atattgaata caaacgaatg
     5221 aatggcagta aatatttact ggcaaaagca tgggataatc gtatcgggac agctgtttct
     5281 ctgcgagtat tagagaatct atctaaagaa gcacacccaa atgttttatt cgcaggaagc
     5341 gatgtgcaag aagaagtagg cttgcgaggt gctcgaacaa gtacacacct tgtaaatccc
     5401 gatattgctt ttgctttaga tactggtaca gcaggagaca ctccaggaat gacaccaaaa
     5461 gaagcagatt cgattttagg aaaaggccca cagattttga tttttgatgc atctatgatc
     5521 ccgcataaaa aattattgaa ttttgttatt gatgtagcag aggaaatgga tattccattc
     5581 caatatacag tgatttctgg cggaggaact gatgcaggac aaatgcattt gacaagagat
     5641 ggtgttccgt ctgtagccat taccgttcct gttcgttatc tgcattccca tacatcagtg
     5701 attcatgaag atgattattt gaatacagtg aaactagtca cagaattagt gaaacggctt
     5761 gatagtgaaa cagttgaaaa aattcggagt tatgaataat gaataataga atacctattt
     5821 ctgaatatcg gaaaaaagca cgagtttctt tggaaaatca atggggaatc aatacttggc
     5881 ttgttttctt atctataata tttacgagcg tcatccagaa cttttttgac ggtttcagtc
     5941 cttttccgga agaaagcatg cagcagcact tattgagttt tctagtcgat aatttacttt
     6001 tattcgcatt caactatgca ttgttttaca ttgcgctagt agttgtacgg ggtggcagac
     6061 caaaaagtaa ccttttattt tcagttttcc aaagaaagta ttttggttcg atacttggca
     6121 tcaatttgat caattcagtc atcaactggc tactcaacag tttaatcttt ctgcccatgt
     6181 ttttaatcag cggagtgagt acttatatgc agctgctttt cagttcaaat gcggtaccta
     6241 cgggtgtcat gccagaagat tttcttgata tcagctttgt tttatcgatg atagggatga
     6301 tttttgtttc agcactcatc atgagtattg ttggcggttt attccaattt gctgcatgga
     6361 caaagttcga ctatcctgat ttgtcggtta ttcaatgttt gaaatatggc tggtttttat
     6421 tgaaagatag aattggtacg tatattttgt tgcagctgtc ttttatcggc tggtatttgc
     6481 taggagcaat cgctttgttc atcggtcttt tgtgggtcat tgtttatgtg aatgtgtcag
     6541 ttgcagaatt ttatgaacaa gcacggattg aaaaaggagg tccagacgcc tattttactt
     6601 agcatgtaag cagtttattc atcaaaaaaa taacttttgt ggtaggataa atctttgagg
     6661 gggaatgtac gatgaaatta actgttttag gatgcttagg agcgtatcct tataaaaatc
     6721 aagggacaac aggttatttg cttcaatctg acgggttcaa tttgttgatc gatgcaggta
     6781 gtgctacgct gatcaaatta caagaacacc ttgatccact tgatctagat gctgtgatcc
     6841 ttagccatta tcatcatgat catattgctg accttggtgt attacagtat tactggcaac
     6901 ttcatcctaa gagaaatccg gagacattgc tttcaattta tggacacaca aaagattcca
     6961 ttcactttga agaattgacg ttggaaaatg tctcaaaagg acatccttat tttgaagctg
     7021 aagaattaaa aattggtcct tttctgatta cttttatgga aacaatccat ccagtagtat
     7081 gttacgccat gcgtatcgta gaagaaacaa cggggaaagt attcgtattt acaggtgatt
     7141 caggttattt ggaaagtttc gctgattttg cgaaagatgc tgatttgttt ctagcagata
     7201 cctatctttt tgaaggaaat gaacatcatc atgcccattt cacttcaaaa gaagctggag
     7261 aacttgcgaa atcagctaac gtaaagaaac ttgtacttac acacctacct cagtttggtg
     7321 atttagaaca attgaaaaat gaagcgcaaa aggcagcagg caaagggatt gatatagaac
     7381 ttgctgcagt tgacaaagtg tttaacattt aggaggaaga acatgatttt tgtaccaaat
     7441 gaaaaccacg acccaagaat caatttagca attgaacaat atttgctgca agaaatgcct
     7501 ttagatgaac ctattttgct cttttatatc aatgaacctt caatcatcat cggcagaaac
     7561 caaaatacgc ttgaagaaat caatcgagat tatgtggaag aaaatggcat tcatgttgtc
     7621 cgtcgcttaa gtggaggcgg agctgtctac catgattttg gaaacctgaa ctttagtttt
     7681 atcatgcctg atgatgggga ttcattccgt gactttgcga aagtaacaca gccaatcatc
     7741 caagcacttc atgaattggg tgttgctggg gcggaattga aaggccgaaa tgatctagtc
     7801 attgatggca tgaaattttc tggcaacgcg atgtatgcta ccaacggtcg gatgtttgct
     7861 catggaacaa ttatgttcga ctctgatatc aatgaagtag tcaatgcatt aaaagtaaaa
     7921 aaagacaaaa tcgaatcaaa aggaatcaaa tcgatccgtt ctcgtgtaac gaatatcaaa
     7981 ccatttcttc cagaagaaaa gcaaaaaatg acaacagaag aatttcgtca agaaatcttg
     8041 ttgaaaattt tcggcgtaga gtcagtagaa gaagtaaaaa cgtacgaatt gactgatgaa
     8101 gattggaaaa aaatcaatcg aatctctgag gaatattatc gaaattggga ttggaattac
     8161 gggaagtctc cagatttcaa tttctctcgc caaaaacgtt tttctatcgg ttcgattgaa
     8221 gtccatttga atgtctcaga aggaatgatc aaagaagcaa aaatctttgg cgatttcttc
     8281 ggactaggag atatctctga cgtggaaaaa aaattggttg gacagaaata tgataaagca
     8341 tctctatcaa aaatagtcag ccaaattgat gtgaagaaat attttggtgc gatagaacca
     8401 gaagaattgc ttaccttact ttattgaaaa taaaaaatag cggaatccac atatatggat
     8461 tccgctattt tttatacagg attcgggtaa tttcgagaga gttgaacagt gatccgatca
     8521 atgaaagtat ctttttcctg cgttagtgtt cctgtgtaac gatccgctac gtctttcatc
     8581 atcattaagt caaaacgttc ctgatcatct aattcttctg tgatgcctcg actaaatagg
     8641 tttgtatcgt aatgtgcact atgttcgcaa caaatgacga aatgttcctc ttgttcgtgt
     8701 atcgacaaat aaatttttcg atacagtgga ttttcgatgg catgagtttc tctaaaggaa
     8761 tgctctaaag cgtggatcaa aagttgagcc agatcgtcat cgttgatagg aagttcttcg
     8821 ggaagatctg cttggaaggt tatctgaata ttccgtttgg cagctaactt ctggtaacgc
     8881 gctaagataa gatttgtcac ctggtggttg caaaaagaac acagcatttc gaaatttttt
     8941 gcttcttcta atatcttttc cacatagttt ttagcttttg tttggtccgt atgcattaaa
     9001 tcttgcagat tctccatctc ttggacaaat tccaggcgaa gtgtatttac ttcttggata
     9061 tgctgtcgaa gctgctcata atgttgctcc aacaaatcat ttttgatttt taagaaatct
     9121 acttgtctcc gatctttgtc ggtatttaag atgtgatcga ttagattgta gctgataata
     9181 acgattagaa tgataatata taaaatcgta gaccaattga tcgttgtttg atagatacct
     9241 aatgccgttt ggatgtaaat caagcaatgg ataatggcta ccaagacgat ccaaggtgta
     9301 caagctacat aaaaacggtt attcttatat gcttcgccaa tgcatgcaag tgaagtgact
     9361 aaagtgctta taacactagc aatattagca atcggcatta attcaggaag ttctgcagga
     9421 gtaaaagttg cataaagcag aacgccgata aaaatcaaaa gttggaacgt acaaaaaatt
     9481 ccgtaccatt ttttatagta aaacattcgg ctccaatagt agcagatgat gaaagctgac
     9541 gttaagataa taaataaatg agataaaaaa gaatgaacga atggttcaaa aagaatacca
     9601 cttaaaatat cttctgatac agcttcaaat cctaatgcca aagcaaagca acttaagaga
     9661 ataagtgagt attcaagacg tttttgttta tacatcaagt agatcgtata gaaaaaggtc
     9721 cctatagcga taaaaacagc gattatcatc gtcaatactt gcggcactga ttgtgaataa
     9781 ataaaactaa tcaatggtcc agttgttcct agataaactt tcggtggcag tccagcatag
     9841 ttctgataag gagatgagac tgtaagggtc aatttttttc cgatataatt tgctggtaaa
     9901 tctatcacag ccaatgagag tccgggattg ctttcttcct gataatcttt ccgttggtat
     9961 aaaatctttt cgcctagata tacagtaatc cattgatggt ttcctttgat tagtaaattt
    10021 gcttcgtcca acggtttttc aagtgtcgtt tccataatga atgtttcgtc tttatccacg
    10081 gtaggaatcg tacgagtgta cttgctgtca aacttaaaat caggatcatc ttttgtatag
    10141 aaaacccacg aatcttttat ttctttcatt tgattgctgt ccgcaggaga aaaaataaaa
    10201 taaaagaaat aagcaattcc tgttaaaaat aacaatgata atataatgat tatgctccac
    10261 tttatacgta aataaaaaga ttctttttta ttatatatgt tattaaaaaa agacataaag
    10321 gtattccttt ctaaaacttg taatgttagg gtaaacgttt tttaaactaa cgtcaattat
    10381 ttataataaa aaatgagata aagatgagga
//
LOCUS       DAXWLB010000101        10371 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXWLB010000101 DAXWLB010000000
VERSION     DAXWLB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.101"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            256..531
                     /locus_tag="ACGE4H_001932"
     CDS             256..531
                     /locus_tag="ACGE4H_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365335.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2622
                     /locus_tag="ACGE4H_001933"
     CDS             544..2622
                     /locus_tag="ACGE4H_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8365336.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2785..4668
                     /locus_tag="ACGE4H_001934"
     CDS             2785..4668
                     /locus_tag="ACGE4H_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8365337.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4680..5627
                     /locus_tag="ACGE4H_001935"
     CDS             4680..5627
                     /locus_tag="ACGE4H_001935"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFF8365338.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="ACGE4H_001936"
     CDS             5647..6174
                     /locus_tag="ACGE4H_001936"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFF8365339.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="ACGE4H_001937"
     CDS             complement(6237..6890)
                     /locus_tag="ACGE4H_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFF8365340.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="ACGE4H_001938"
     CDS             7022..7864
                     /locus_tag="ACGE4H_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8365341.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="ACGE4H_001939"
     CDS             8022..8918
                     /locus_tag="ACGE4H_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFF8365342.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="ACGE4H_001940"
     CDS             8921..9634
                     /locus_tag="ACGE4H_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFF8365343.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="ACGE4H_001941"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="ACGE4H_001941"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFF8365344.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..>10371
                     /locus_tag="ACGE4H_001942"
     CDS             10169..>10371
                     /locus_tag="ACGE4H_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFF8365345.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEK"
BASE COUNT         3596 a         1771 c         2150 g         2854 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gcattcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat a
//
LOCUS       DAXWLB010000102        10195 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXWLB010000102 DAXWLB010000000
VERSION     DAXWLB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.102"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            45..2741
                     /locus_tag="ACGE4H_001943"
     CDS             45..2741
                     /locus_tag="ACGE4H_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8365346.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            2806..3306
                     /locus_tag="ACGE4H_001944"
     CDS             2806..3306
                     /locus_tag="ACGE4H_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365347.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            3318..3812
                     /locus_tag="ACGE4H_001945"
     CDS             3318..3812
                     /locus_tag="ACGE4H_001945"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8365348.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            3833..4642
                     /locus_tag="ACGE4H_001946"
     CDS             3833..4642
                     /locus_tag="ACGE4H_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFF8365349.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            4639..5460
                     /locus_tag="ACGE4H_001947"
     CDS             4639..5460
                     /locus_tag="ACGE4H_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8365350.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            5511..7610
                     /locus_tag="ACGE4H_001948"
     CDS             5511..7610
                     /locus_tag="ACGE4H_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFF8365351.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            7607..8725
                     /locus_tag="ACGE4H_001949"
     CDS             7607..8725
                     /locus_tag="ACGE4H_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFF8365352.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            8764..9642
                     /locus_tag="ACGE4H_001950"
     CDS             8764..9642
                     /locus_tag="ACGE4H_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8365353.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            9788..9985
                     /locus_tag="ACGE4H_001951"
     CDS             9788..9985
                     /locus_tag="ACGE4H_001951"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFF8365354.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            9969..>10195
                     /locus_tag="ACGE4H_001952"
     CDS             9969..>10195
                     /locus_tag="ACGE4H_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365355.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKL"
BASE COUNT         3130 a         1676 c         2280 g         3109 t
ORIGIN      
        1 atatctgagg caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata
       61 gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa
      121 aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata
      181 tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca
      241 gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg
      301 ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag
      361 cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag
      421 ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg
      481 gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa
      541 aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt
      601 cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg
      661 gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga
      721 aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg
      781 aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg
      841 aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt
      901 ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt caaaatgaag
      961 cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc
     1021 atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg
     1081 tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt
     1141 taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa
     1201 atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa
     1261 ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag
     1321 tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga
     1381 tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga
     1441 cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca agctataaga
     1501 cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat
     1561 gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac
     1621 tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt
     1681 tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg
     1741 gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg
     1801 aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag
     1861 atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac
     1921 ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga
     1981 atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat
     2041 actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga
     2101 tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt
     2161 tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg
     2221 acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata
     2281 ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc
     2341 ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt
     2401 tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg
     2461 aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac
     2521 taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa
     2581 aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat tatgtttcta
     2641 gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct tatgagcttt
     2701 cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa aaaagtgtct
     2761 aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat taaaccaatg
     2821 tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa aggaattaac
     2881 catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt tcttagcagt
     2941 attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta tgtggatgag
     3001 tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca ggatcagatt
     3061 tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa aacctttttg
     3121 gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct gctcgaaata
     3181 gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa taaatgccaa
     3241 caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga ggaagatttt
     3301 ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta attcatggac
     3361 aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta gcaagcgatg
     3421 aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca tcgaatgtca
     3481 aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg cgggcgaagg
     3541 acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt gcgaaagctt
     3601 tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca ccgccatcgg
     3661 aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt caagacttaa
     3721 aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg atggaaaaac
     3781 gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa ttatgtatga
     3841 tgcatttgta gtcgctttgg ccgtttttat tggagttgct ggacatgaaa tgttcgggat
     3901 gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt
     3961 gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg tcgtcaatat
     4021 cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag cgttcgcaat
     4081 caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg
     4141 attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt
     4201 caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct ttatgtggtt
     4261 tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt
     4321 tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg
     4381 aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga gtaaaaatat
     4441 tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc
     4501 aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa
     4561 aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga
     4621 agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg
     4681 gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg
     4741 ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa
     4801 gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc
     4861 gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt
     4921 tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt
     4981 ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta
     5041 gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat
     5101 tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa
     5161 aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg
     5221 atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca
     5281 atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt
     5341 gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt cggcgtcatc
     5401 aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag
     5461 taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta
     5521 atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac gacggaaatg
     5581 tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct
     5641 tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg attcaaacgt
     5701 tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac
     5761 agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt
     5821 tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata
     5881 ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata ctggctaata
     5941 agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc
     6001 aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag caaaatgtgt
     6061 tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca
     6121 ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg
     6181 aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc
     6241 caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg
     6301 gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc
     6361 gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt
     6421 tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat
     6481 ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc
     6541 tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag gttctactga
     6601 atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt
     6661 ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc
     6721 tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga
     6781 gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa
     6841 tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa
     6901 caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa
     6961 gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca
     7021 ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat cagaacacca
     7081 tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt
     7141 ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc
     7201 acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg
     7261 gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact
     7321 ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat
     7381 atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt
     7441 tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg
     7501 agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc
     7561 ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa
     7621 aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg
     7681 cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg
     7741 cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat
     7801 aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg
     7861 tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt
     7921 aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt
     7981 ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa
     8041 aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta
     8101 cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga
     8161 acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta
     8221 ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga
     8281 gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga
     8341 agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc
     8401 gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag
     8461 agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc
     8521 acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc
     8581 ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt
     8641 tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac gtcatttcac
     8701 tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag ggaggaacct
     8761 atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc agtcggtact
     8821 gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt tgaaacaatg
     8881 aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat cggggtcggc
     8941 tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat cacatcaaca
     9001 ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata tttcaatatt
     9061 ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt tgctggttct
     9121 ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc
     9181 ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc
     9241 aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag
     9301 gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg
     9361 gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca
     9421 acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac
     9481 atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca
     9541 ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga
     9601 ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg
     9661 atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc
     9721 gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg
     9781 aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat
     9841 attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt ctgtggtaat
     9901 agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca taatccttgg
     9961 cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata aatcgtaatt
    10021 ctattataga gagttgtgat tataaaaaga tagcaagaaa aaaaaagaaa gtttctatac
    10081 attttaaata ttttattcct atttctattg ctaaaaaagt agaaattcta tatttacctt
    10141 taactctcgc ttctcttgca aatgtagtta gcattttaat cgataataaa ctcag
//
LOCUS       DAXWLB010000103        10184 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXWLB010000103 DAXWLB010000000
VERSION     DAXWLB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.103"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1485)
                     /locus_tag="ACGE4H_001953"
     CDS             complement(<1..1485)
                     /locus_tag="ACGE4H_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFF8365356.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYP"
     gene            1644..1835
                     /locus_tag="ACGE4H_001954"
     CDS             1644..1835
                     /locus_tag="ACGE4H_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365357.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2038..2955
                     /locus_tag="ACGE4H_001955"
     CDS             2038..2955
                     /locus_tag="ACGE4H_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFF8365358.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2948..4021
                     /locus_tag="ACGE4H_001956"
     CDS             2948..4021
                     /locus_tag="ACGE4H_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365359.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4169..4687
                     /locus_tag="ACGE4H_001957"
     CDS             4169..4687
                     /locus_tag="ACGE4H_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365360.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4668..5060
                     /locus_tag="ACGE4H_001958"
     CDS             4668..5060
                     /locus_tag="ACGE4H_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFF8365361.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGESATTGAKEIKLNDGQSVIFNLSKL"
     gene            5136..6071
                     /locus_tag="ACGE4H_001959"
     CDS             5136..6071
                     /locus_tag="ACGE4H_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFF8365362.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6204..6983
                     /locus_tag="ACGE4H_001960"
     CDS             6204..6983
                     /locus_tag="ACGE4H_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFF8365363.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            6976..7293
                     /locus_tag="ACGE4H_001961"
     CDS             6976..7293
                     /locus_tag="ACGE4H_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFF8365364.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7537..9147
                     /locus_tag="ACGE4H_001962"
     CDS             7537..9147
                     /locus_tag="ACGE4H_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8365365.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9304..10152
                     /locus_tag="ACGE4H_001963"
     CDS             9304..10152
                     /locus_tag="ACGE4H_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8365366.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3200 a         1790 c         2061 g         3133 t
ORIGIN      
        1 agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt gttcatcatt
       61 tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta gtttatcata
      121 agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca agacttcatt
      181 gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt gtattgtttc
      241 aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat gttttactgc
      301 atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg taatgatgac
      361 ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac tttgcgcttg
      421 aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag gacgtaaacc
      481 gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca atcctttttt
      541 gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg cttcttggac
      601 aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag taggagattg
      661 ataaacagat tctttaccag taattttttc aataatacgt ttgattacag gaaatgtctc
      721 aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga aggaatcgat
      781 catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca gcggatgttt
      841 caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg catgtccttt
      901 ttggctttca tgatacagtt ggttcaagca agtagcgagc tttccacttc ctgggccagg
      961 agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc caaaaccatc
     1021 ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt gtttatagac
     1081 cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct gtccactata
     1141 gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat cgattaaccg
     1201 caagatatcc atatcataag taatcccata atcaccacgg atcttattac gttcaatatc
     1261 tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca gcaatttgat
     1321 tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt caactaactt
     1381 cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa tatacgctga
     1441 ctgctcttca atatattttt ctgaatcaaa accaattttt ttcactaggt tccctcctta
     1501 agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc gaaattcctc
     1561 aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat tggtatgata
     1621 gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac gcatataaac
     1681 actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc gaattggaac
     1741 aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag catcagcgca
     1801 ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg tcatcggatt
     1861 cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt accaaatttt
     1921 ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt ttacagaaaa
     1981 tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc aagtagtatg
     2041 aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac gaacgcggat
     2101 caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc aagcatgaat
     2161 ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga agaagatatc
     2221 cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag aagctcaaat
     2281 gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt tttacaggaa
     2341 tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta caaaatcggc
     2401 acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga aatttttgtt
     2461 ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac ggactatgac
     2521 aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat tgaacgttta
     2581 gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa taaaggattc
     2641 ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa gcaagtacta
     2701 tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat ccagcagcaa
     2761 aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat cgtagaaaaa
     2821 gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca agcaatccat
     2881 gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa tgaaggaggt
     2941 tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac aaacagaaga
     3001 tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg atgacacaga
     3061 agaaatccct atgctagagg aaacaaaaat acatgaaaaa gaaaaagtaa ctccgacgaa
     3121 tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg ggtatttaga
     3181 aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg aagcagaatt
     3241 agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat tattagaatc
     3301 atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga caagcctgat
     3361 cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg aacggtcaaa
     3421 ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt tgaatggaca
     3481 aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc aagtggaatc
     3541 gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag atgaccgggt
     3601 gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc agatcacgat
     3661 attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt ttaccgttaa
     3721 tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg aatttgaatt
     3781 gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat cctacgatat
     3841 ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt ctttaacgat
     3901 aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact acccacgatc
     3961 tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga gaaagtcctg
     4021 attggataag ttggattacc agatgtcatt cagataattc tgttccaaag gacttgcggc
     4081 agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac gtgaataact
     4141 tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac agcttagctt
     4201 attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga atttccagcc
     4261 aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg cgttaattgt
     4321 catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat gggtcatcgg
     4381 ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct tgagaaaaac
     4441 agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca tggtttacgg
     4501 aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt ggatctatta
     4561 ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt tctttcttgt
     4621 ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg aaaaaaataa
     4681 tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc agtacaacct
     4741 atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa gcaacaatca
     4801 cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt ggtcttcaaa aaaggcgatg
     4861 atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc ttcatcactt
     4921 cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt actgtggacg
     4981 gggaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa tcggtcattt
     5041 tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata ttgccataat
     5101 tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa aaaagcggaa
     5161 tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc aaatttgtcg
     5221 gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt atcgttgatt
     5281 gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga cagagcgagt
     5341 tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc gcaatgatgc
     5401 ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg acgcatccat
     5461 cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt ttggtaatcg
     5521 aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact gaaaaacttt
     5581 ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt tttacggaag
     5641 acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg acttatgcaa
     5701 ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg ctgatgggag
     5761 ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc gtgacagcaa
     5821 gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg ctgatcgata
     5881 taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca gatatcgttg
     5941 gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat atgcgtcagt
     6001 ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac ccaaattatt
     6061 ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata taatcttgtt
     6121 tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc tgtttatttg
     6181 ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg cttcaagctt
     6241 tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct tgcggagtat
     6301 tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt ctggtctttt
     6361 ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca tttgcaacga
     6421 cattattgat ggttttcttt ttagaattac gctatacatt attaggagca agtcttgccg
     6481 gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca ctgaatgatg
     6541 aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat aaaagaaagg
     6601 ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg atcggtaatt
     6661 ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg acggccatgt
     6721 tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca ggtttattct
     6781 caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga ttgattgcag
     6841 caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa aaagaaaaag
     6901 ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac ttcccggaac
     6961 aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg tctgttcatc
     7021 gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa agtgccatcc
     7081 tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt agcttttaaa
     7141 gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat agctgcaatg
     7201 attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt tttgacagga
     7261 cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta aaaacagaga
     7321 ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag tcaattctgg
     7381 atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc aagctctttt
     7441 ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata atagggaaga
     7501 tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt gatgcaagga
     7561 acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt ctatttgatt
     7621 ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac attgtataat
     7681 gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt tccaaatgcg
     7741 attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg tcggctgatt
     7801 tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc actgatgtat
     7861 ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa tgggatacta
     7921 gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc aatgagagga
     7981 tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat cgaacaggct
     8041 gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac tgacggaacc
     8101 atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt agctgcgatt
     8161 atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga ttttttgatt
     8221 tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat tcgggaaaca
     8281 ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga ccaagtaacg
     8341 atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact ggaattctta
     8401 ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc catggtagga
     8461 acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga tcggatacaa
     8521 atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct tccttcattg
     8581 actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta tgatcctgaa
     8641 agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt attcacgatc
     8701 ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt aactgtagaa
     8761 gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg cggatacggg
     8821 atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta tcactttatg
     8881 tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt aaaaactttc
     8941 cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct gaatcagcga
     9001 ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg aagtattggc
     9061 ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt gaaaaatgtt
     9121 aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt cacttgaaaa
     9181 acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag tttcatatct
     9241 aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga ggaaattaaa
     9301 cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg tatcaagaac
     9361 catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat ttataacgaa
     9421 ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa agattttcct
     9481 actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt gaaagagaaa
     9541 gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt tgtcaacaca
     9601 ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga ctctaagatc
     9661 actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga tgaaggtcag
     9721 tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta tgcttatctg
     9781 attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg tgcagcgctg
     9841 attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa aattgtttta
     9901 ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat tattggtaga
     9961 agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa taatttagaa
    10021 gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga aatcgaaatt
    10081 ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg cattgccatt
    10141 tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atat
//
LOCUS       DAXWLB010000104        10090 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXWLB010000104 DAXWLB010000000
VERSION     DAXWLB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.104"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(242..754)
                     /locus_tag="ACGE4H_001964"
     CDS             complement(242..754)
                     /locus_tag="ACGE4H_001964"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFF8365367.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(768..3065)
                     /gene="recJ"
                     /locus_tag="ACGE4H_001965"
     CDS             complement(768..3065)
                     /gene="recJ"
                     /locus_tag="ACGE4H_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFF8365368.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3207..3650)
                     /locus_tag="ACGE4H_001966"
     CDS             complement(3207..3650)
                     /locus_tag="ACGE4H_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFF8365369.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3651..4439)
                     /locus_tag="ACGE4H_001967"
     CDS             complement(3651..4439)
                     /locus_tag="ACGE4H_001967"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFF8365370.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4436..5377)
                     /gene="rnz"
                     /locus_tag="ACGE4H_001968"
     CDS             complement(4436..5377)
                     /gene="rnz"
                     /locus_tag="ACGE4H_001968"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFF8365371.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5554..6141)
                     /locus_tag="ACGE4H_001969"
     CDS             complement(5554..6141)
                     /locus_tag="ACGE4H_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFF8365372.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6362..7675)
                     /gene="obgE"
                     /locus_tag="ACGE4H_001970"
     CDS             complement(6362..7675)
                     /gene="obgE"
                     /locus_tag="ACGE4H_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFF8365373.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7972..8982
                     /gene="gap"
                     /locus_tag="ACGE4H_001971"
     CDS             7972..8982
                     /gene="gap"
                     /locus_tag="ACGE4H_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8365374.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9067..10002)
                     /locus_tag="ACGE4H_001972"
     CDS             complement(9067..10002)
                     /locus_tag="ACGE4H_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8365375.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2780 a         2298 c         1747 g         3265 t
ORIGIN      
        1 cgggggtgtg acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca
       61 gtttttcggt attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt
      121 gcttgtttcc atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt
      181 tgagggtcgc tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg
      241 cttaatattc cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat
      301 ccatcaattc gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt
      361 cgatcgtcgc tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt
      421 gacctggttt aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat
      481 aagtcacttc gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga
      541 ctcctaactc ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta
      601 ccatatcaat tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag
      661 cgtcaccatc tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt
      721 cgggaatact ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac
      781 catttcttta tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg
      841 agccgtcgct ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt
      901 tgtaaaacac cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa
      961 attaataatt tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata
     1021 tcaattcgtt cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct
     1081 accccattca aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa
     1141 taagaccgaa aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga
     1201 gagtttggtt ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc
     1261 gcagcttcct ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa
     1321 aggctacctt ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact
     1381 tgcagaccac ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat
     1441 tcattgatcg aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga
     1501 gtaccaaaac caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc
     1561 agatgctgtt ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa
     1621 aactgaggca aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc
     1681 tctactgtta cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc
     1741 aagtcaattt gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt
     1801 tcacttggca tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct
     1861 cgcatctggt caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta
     1921 gctgatccat cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt
     1981 ttcccagcaa caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata
     2041 tgattctcct tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt
     2101 ctttcttcat tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac
     2161 gtcgcaagta acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg
     2221 ggagcaatcg aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct
     2281 tcaaatagcg cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa
     2341 acaaggattc gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact
     2401 aaatccaaaa attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact
     2461 ccgactcctg ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc
     2521 gcataagcgt ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca
     2581 ccagcttgct gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg
     2641 atcaactgga ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca
     2701 tgctcaaatc gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct
     2761 ttcattaccg ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt
     2821 tctccactga ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga
     2881 tacggatcat gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca
     2941 tggtaacctc tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc
     3001 atttggagaa aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt
     3061 ctcactttct cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga
     3121 aagaaagaat aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt
     3181 gtacgaccat ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat
     3241 tgagtttttt gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac
     3301 tccgcctgat aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct
     3361 tcttttgctt cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc
     3421 agctgtttga tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc
     3481 agtgcaccaa tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg
     3541 aagccaaaac ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa
     3601 actaaaacaa atccaagaat cacattgctt tgctttttca attccttcac ctactttttg
     3661 ttaaaaattc cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat
     3721 gcaacttcta aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg
     3781 atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt
     3841 ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta
     3901 acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca
     3961 aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg
     4021 atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa
     4081 accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta
     4141 aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact
     4201 tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg
     4261 aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat
     4321 gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt
     4381 tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg
     4441 cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt
     4501 tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg
     4561 cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt
     4621 gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc
     4681 tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg
     4741 tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat
     4801 tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg
     4861 gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat
     4921 gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag
     4981 aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat
     5041 aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg
     5101 gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg
     5161 gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg
     5221 gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa
     5281 cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag
     5341 gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt
     5401 tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca
     5461 aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca
     5521 gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt
     5581 gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg
     5641 attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt
     5701 aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc
     5761 agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac
     5821 ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa
     5881 ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc
     5941 acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg
     6001 tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc
     6061 atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa
     6121 aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta
     6181 cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg
     6241 acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt
     6301 ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg
     6361 cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac
     6421 cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca
     6481 ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata
     6541 agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc
     6601 catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt
     6661 ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa
     6721 ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt
     6781 tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt
     6841 gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt
     6901 cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg
     6961 ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa
     7021 tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta
     7081 gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa
     7141 cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata
     7201 ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg
     7261 gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg
     7321 ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat
     7381 ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca
     7441 tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat
     7501 ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt
     7561 ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt
     7621 ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta
     7681 agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc
     7741 ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac
     7801 cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat
     7861 tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgtat
     7921 taattgagga tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta
     7981 aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa
     8041 gtctcagatg atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca
     8101 caattattgc aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa
     8161 gactcaatcg tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa
     8221 atcccttggg caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca
     8281 tctgaagaaa aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc
     8341 ccagctggtg ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac
     8401 gacaaaatca tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc
     8461 ttaaataatg aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc
     8521 acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct
     8581 gctgacaata ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct
     8641 gagctacaag gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta
     8701 actgaattgg tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc
     8761 aaaaaacaca cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc
     8821 gatgtcattg gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact
     8881 gctggtgatt tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact
     8941 tgccaaatga ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat
     9001 aaaagaagcc tttgactgga tccagtcaaa ggcttctttc ttttcatccc ctacttttaa
     9061 actcgttcaa tgaacatatt ggcttaaaaa ttgttggact acttgctgat atttttcagg
     9121 atctgtcttg tatgcttccg catgctcggc tccttttaca acatactttt cttttggtcc
     9181 cttcgttgca cgatagacct tatctaacat ttcaaatggt acaaatgtat cttcgtctcc
     9241 atgtataaag agcattgggc gctggttttt cttcaattgt tccactgcat ttgcttcacc
     9301 gaaaaagtag ccagcacgga cttttgtcac tagactcgtt acttgcataa gcggaaatgc
     9361 tggaagacta aacatatcgt ctagctgata agcaagctca tcatgaacgg aagaataacc
     9421 gcaatcttca atgattgctt tcacattatc tggtagtttt tctccgctag tcatcataac
     9481 tgttgctgct cccatgctga caccgtatag aacgatttct tgcgattttc cattgttttc
     9541 tagcactttg tttatccact gtacatagtc tttacgctct ggccatccaa atccaatgta
     9601 atccccttcg ctttttccat gtcctcttgc atcaggaaca aggacattgt aacccatatc
     9661 atgaaacatc tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc catgagccac
     9721 aagagctgtt ttttcacttt tcgtttcagc aggaagataa atcgctgata atttcagacc
     9781 gtcttttgaa gtgatggtcc aatcgcttcg gttttctttt gaactaaacc atttatgtgc
     9841 atccgtttct ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc
     9901 agaaggcaca acagcataat tataaaaata attccctgca aacactaacg caacaatcag
     9961 agctaaagcg acgcctaaaa ggattttcat ccaaagtttc ataaatcatc catctcattt
    10021 ctcttttttc atataacctt gaattagtat aatcaaagaa tctctactat gcaatgttca
    10081 tttttataac
//
LOCUS       DAXWLB010000105         9996 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXWLB010000105 DAXWLB010000000
VERSION     DAXWLB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.105"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            254..733
                     /locus_tag="ACGE4H_001973"
     CDS             254..733
                     /locus_tag="ACGE4H_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFF8365376.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            766..2994
                     /locus_tag="ACGE4H_001974"
     CDS             766..2994
                     /locus_tag="ACGE4H_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8365377.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYADAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3123..4766
                     /locus_tag="ACGE4H_001975"
     CDS             3123..4766
                     /locus_tag="ACGE4H_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFF8365378.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4839..5384)
                     /locus_tag="ACGE4H_001976"
     CDS             complement(4839..5384)
                     /locus_tag="ACGE4H_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFF8365379.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5407..5892)
                     /locus_tag="ACGE4H_001977"
     CDS             complement(5407..5892)
                     /locus_tag="ACGE4H_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFF8365380.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5882..6694)
                     /locus_tag="ACGE4H_001978"
     CDS             complement(5882..6694)
                     /locus_tag="ACGE4H_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFF8365381.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6833..8365)
                     /locus_tag="ACGE4H_001979"
     CDS             complement(6833..8365)
                     /locus_tag="ACGE4H_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFF8365382.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8474..9505)
                     /locus_tag="ACGE4H_001980"
     CDS             complement(8474..9505)
                     /locus_tag="ACGE4H_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8365383.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3329 a         1844 c         1924 g         2899 t
ORIGIN      
        1 acaaaaaaga tgttggtgaa gttcaccaac atcaaaaagt atagagccaa aatattccaa
       61 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
      121 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
      181 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
      241 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
      301 agactatgat aaagttgatg gaagaaatgc attaaatatg gatgcaaaag ggctttcact
      361 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
      421 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
      481 agcgatctta gctgcgcaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
      541 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
      601 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
      661 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
      721 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
      781 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
      841 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
      901 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
      961 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     1021 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     1081 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     1141 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     1201 ctagttcaaa ggatcgaagc tgattatgca gatgcaatcc attacagcta ttacccttct
     1261 gaaatcgttc gagcgattga aaaaggctat ttattagaga ttcaagagcc gactgtcatc
     1321 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     1381 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     1441 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     1501 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     1561 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     1621 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     1681 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     1741 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     1801 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     1861 gaaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     1921 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     1981 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     2041 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     2101 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     2161 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     2221 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     2281 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     2341 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     2401 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     2461 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     2521 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     2581 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     2641 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     2701 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     2761 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     2821 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     2881 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     2941 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     3001 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     3061 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     3121 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     3181 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     3241 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     3301 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     3361 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     3421 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     3481 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     3541 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     3601 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     3661 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     3721 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     3781 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     3841 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     3901 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     3961 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaacat gcattaagcg
     4021 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     4081 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     4141 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     4201 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     4261 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     4321 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     4381 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     4441 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     4501 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     4561 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     4621 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     4681 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     4741 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     4801 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     4861 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     4921 cgaggtctga caggtacttg gacatgcttg atatgcatac cgattgatgt gtcaccgata
     4981 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     5041 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     5101 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     5161 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     5221 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     5281 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     5341 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     5401 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     5461 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     5521 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     5581 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     5641 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     5701 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     5761 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     5821 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     5881 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
     5941 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
     6001 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
     6061 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
     6121 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
     6181 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
     6241 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
     6301 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
     6361 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
     6421 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
     6481 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
     6541 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
     6601 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
     6661 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
     6721 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata tttttatctc atccaattta
     6781 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
     6841 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
     6901 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
     6961 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
     7021 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
     7081 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
     7141 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
     7201 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
     7261 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
     7321 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
     7381 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
     7441 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
     7501 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
     7561 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
     7621 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
     7681 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
     7741 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
     7801 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
     7861 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
     7921 gttccattaa ataaagaata ggaatctgtt ttgattttgt tccatttgaa atttctctca
     7981 ctcgcttttt tgtctcatca tcaaataaaa tgaaagggtc ttctaaacga tcgataaata
     8041 cttccattgt tgttgttggt agatgataag aagtcatgac aggcactgca ccaaggtaag
     8101 acacagaaac agctagaagg tatgtgtcaa aagcggaact cttataaata ataactttgt
     8161 ctccggattt cactccaaga tgagccaatt gataagcccg tttcaacaca ctttcgcagc
     8221 attttttgta agttgtttca agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga
     8281 taatcggggt ctcggaaaag ttagctgcag cttcttgaaa attcgtaaat aagttcaaag
     8341 gggaatacgt tatggtctcc atcattcaca ctccattatc ttttgttaag taatatacaa
     8401 attcaagata atcgtaacat aatcctttat ataataacat agtttattgt gaaaaaaaga
     8461 tcatacattt gttttatatg gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa
     8521 aatcaaccat cttttcccag atatcttgaa aagaatggga tgagaggtat cgattaccac
     8581 ataaataatt taccgcgtca gcatcatcta aatatagttc tgcataataa cgatatgttt
     8641 ttctgcatag ttctggcgat acgtttttat atactgtaat tttgctcaaa atttggcgaa
     8701 tagtccgagg agaaatttgc tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg
     8761 tattttgtgc tgttaactta aaatagttct ccagaaatgg catcgcttct tttggtattg
     8821 gtactttatt cagtctgttt tcttttctat tcagattaag aatctgcttg gtcaaagata
     8881 catctttacg tttaagttct actacttcat ggatctttaa tccgcaatac agaagaatgg
     8941 cagtaattgc aaggttcctc ggtctattct tttcttcaaa gctagcttct ctgcttgttt
     9001 gaaagccttc aatcgggata aacaaatgct caaaatcttt gaaaagttct gcatcgatat
     9061 atcgcggagc tgctttggtt gtttccggta aacttgtacc caaaagagaa atctcttctt
     9121 caatcagtcc tttttgatac aaaaatcgta aaaaatgatt taatgaatat tgcttccttg
     9181 aaatcccgta atagctattt tcaaacaatt gctccgtttc ttggttcgct gtagtttgga
     9241 acgaacgagt atgtcgatag attttagcta agaaattctg ataatcttgt ttttctaaat
     9301 ggataatcag ctgatcatcc acttcttcag aaaatacttt tctcaaataa tcagtaaaca
     9361 gatgaaaatc ttttgcgtat tcatagcgac tgcctaatga aagggaagaa cgattttctt
     9421 ctaaaaactg ttgaaagacg gaagggagca agtgtgtacg ttgctttaac ttttcttgca
     9481 gttgttttct agttttgctg tccattacaa attccctcca atagtgttca ttttctatat
     9541 tttaccgcat ttcatcaaat gaagatagac agtttgtctt gatcttagga agcaaatttt
     9601 taactaagta ctagctaaaa gatatgaagt aagaagatac atttttagtt attttattag
     9661 tgcagtcatt cttttgatct tttaattgat ttatcctctt tttagctatg tggttacttc
     9721 atgagcaatg aaatggattt ggttcgttct aatggcagga ggctctttct tttatttccg
     9781 aaaaattaga aaacattttt tcagaatcga ttggctctcc atttattcaa taatggtttg
     9841 tctaacgata ttgttgggat aattcttaaa gaagtattag aaatagttgg tacttcatta
     9901 ctttacatga agatttattc tatggtagga gcaattatat tgagaattaa tcctttttta
     9961 tataagttgt ttgcttattg atggattgga taaatt
//
LOCUS       DAXWLB010000106         9977 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXWLB010000106 DAXWLB010000000
VERSION     DAXWLB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.106"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            20..958
                     /gene="yidC"
                     /locus_tag="ACGE4H_001981"
     CDS             20..958
                     /gene="yidC"
                     /locus_tag="ACGE4H_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFF8365384.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(1139..1543)
                     /locus_tag="ACGE4H_001982"
     CDS             complement(1139..1543)
                     /locus_tag="ACGE4H_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365385.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(1559..2368)
                     /locus_tag="ACGE4H_001983"
     CDS             complement(1559..2368)
                     /locus_tag="ACGE4H_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8365386.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(2355..3245)
                     /locus_tag="ACGE4H_001984"
     CDS             complement(2355..3245)
                     /locus_tag="ACGE4H_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8365387.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(3258..3737)
                     /locus_tag="ACGE4H_001985"
     CDS             complement(3258..3737)
                     /locus_tag="ACGE4H_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFF8365388.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(3754..5520)
                     /locus_tag="ACGE4H_001986"
     CDS             complement(3754..5520)
                     /locus_tag="ACGE4H_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFF8365389.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFATGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(5566..6507)
                     /locus_tag="ACGE4H_001987"
     CDS             complement(5566..6507)
                     /locus_tag="ACGE4H_001987"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFF8365390.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(6504..7499)
                     /gene="lacD"
                     /locus_tag="ACGE4H_001988"
     CDS             complement(6504..7499)
                     /gene="lacD"
                     /locus_tag="ACGE4H_001988"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8365391.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(7492..8685)
                     /locus_tag="ACGE4H_001989"
     CDS             complement(7492..8685)
                     /locus_tag="ACGE4H_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8365392.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(8703..9431)
                     /locus_tag="ACGE4H_001990"
     CDS             complement(8703..9431)
                     /locus_tag="ACGE4H_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365393.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         2829 a         2199 c         1721 g         3228 t
ORIGIN      
        1 ataaaagagg aagtatttta tgaataaatg gaaaaattgg ttattgggct ctgggcttgt
       61 cacgatcatg cttttcttgt ccggttgtgt ccgcatggat tcaaatggta accctgacac
      121 ttcaggaatc atttaccgta tattggttca cccaatggga caagcgatta catatttagt
      181 ggaaaacttt aattggtcct acggctgggc tgttatcttg atgacagtca tcgttcgtat
      241 catcattttg ccattaggta ttagtcaatc gaaaaaaaca atgatccaat ctgaaaagat
      301 gcaagcattg aaaccacaag ttgaagctac gcaacaaaaa ttgaaagctg cgacaactcg
      361 agaagaacaa atggctgcac aagctgaaat gcaacaagtt tatcgtgaaa atggcttaag
      421 tatgactggt ggtatcggtt gtttaccgct tctgatccaa atgccgatct tttctgcgct
      481 atatttcact gctcgctata cagaaggcat ccgtgaatca accttttacg ggatcgacct
      541 aggtaaccct agcttagttt tagtagcgat tgccggtatt gcttatttac ttcaaggcta
      601 tatctcaact atcggtatcc ctgaagaaca aaagaaaacg atgcgtacga tgttgatcgt
      661 atcacccgct atgatcgtct tcatgtcgat cagtgcccca gctggtgtca ctctttactg
      721 ggtagtcggt gggatcttta gttgtctaca aacattcatt acgaatgtca tcatgaaacc
      781 aagattaaaa gcgcaagttg cggaagaatt aaaacaaaat ccgccaaaac aagtggtgac
      841 gcctcgtaaa gatgtcacac ctgctgaacc tgcgaagcaa acaaaaaaac aagtacaagc
      901 accaaaaaac aatgcgaaga atggtcggaa tgctggtaaa cagcaaaagc gtaaatagtt
      961 atttgtaact agcttcttcg atcgacccag atcatttctg attttatgac gataaaagcc
     1021 aacgtactgt gaaatataga aaactatatt tcgcggtacg ttggcttttt tatacacggc
     1081 ctctttatac atccgtattt atatccagtt gtatcaccag tcaagaagtt gtgcttgatt
     1141 attcttcttc atcgtctccc atgccagcaa tcacttggtt cactgaaacg atactctcac
     1201 gacccgaacc aataaaatct ggttctgctc caatcatttg cgaattgatg aattcgatga
     1261 ccatcggcaa attcatgcca gcatatagtt cgatcgtttc accttccatg attagcttgg
     1321 aaacggtatt tgcaggtgtc ccacccaaca gatcgcaaag cactacgtaa tctttcaatg
     1381 gtgctgtaac ggagaggaat ctttctttat acgtgtccgc gccatcttca ggcaataaag
     1441 ggatcgtata gatattttct tgtggtccca tgatcatttc tgtactgttt tttagttctt
     1501 cacaaaattt accatgacta atcaagacta gttgtctact catctttcca attctccctt
     1561 atttcgaaat cacacctaat gcagataatg ctacacatac gatcaatacg atgaatattg
     1621 ctcgagtagg tgtcatattt ttcttaccta acatccaata aacgccacca acgattccag
     1681 ctggtaaaag tttaggtaag atcatatcga tatagttttg catattcaac gttacatccc
     1741 caatcgttgg tgcccatgag aatttaacat tgatcatggt tgctactaac gctccaacca
     1801 tgaaaactcc tagtaaagta gccgcatctg tcaatgcagt caagcgtgat tgcatcgtgg
     1861 taactagaga aatcccttct ttgtaggcaa attctagttg tttccaacga aagaccatga
     1921 cagcgacctg agcaaggatc cagataagga tacctactgg attaccatct gttgccatat
     1981 ttgcggctaa agctccaaat atggtcggga tcaatgccgc aaatacagaa tcaccaattg
     2041 cagcgaagga acccatcaat cctgctttca tcccagaaac agtttccttt gaagagactc
     2101 cttctttttc ttcgatagca agatcgatcc cagtaatgat cgtgttaaag aaattagacg
     2161 tattaaaaaa ttgcgtatgt gttttcatca tttcttttaa ttctggtgta tcatcaccat
     2221 agatttttct aagttgtggc aagatagtat acagataacc tgaaccttgc attctttcat
     2281 agttccaacc taattggaaa gtaaacaaac ttctacgatt gatctgctta aaatcttctt
     2341 tggtcaattt ataattagat ttcgtcatct tcgatttccc cctcatcaga ttgtgattgc
     2401 tgacttacta ctggatgttg gtcattcttt tgatcattct tataactgat cgttgccagt
     2461 gctagaccaa tcaatgccac agcaagcatc ggtaatgaat tgaatgttcc tgggaaatct
     2521 ttgaccacgc tagccacact tgttcctaac aattggacat tcgagaacac tgttgctaat
     2581 aacgctgtga tcgtaaatcc taaaatcaga taagaaatgt gtttctttac tggtaggtag
     2641 cgtagtaaaa ttgcaaaccc aactgctggt aagactgccc ctgcaacaga taagccatct
     2701 cctagccatt ttaggtcgcc gtttaaaaca gccacaacat tttggactaa gccaccaccg
     2761 aatgctaggg ccaagaatac aggaaccatt ctggaaagtg cccaaggtaa tgcccccatc
     2821 aagaagttcc gttcaattgc tttgtaattc attgtttcca catgttgatc gatccgatgt
     2881 gcaaaatacg tattcgcaaa acgtgccaaa atatctaatt ggatcatcaa acttgctaca
     2941 ggaaccgcga tggccgcaat cgcttgttct gggttcatcc ctagagaaat ggagaaagct
     3001 gttgctaaga tcgttccaga gttggcatcg atttttgatg caccaccaaa tgtccctacc
     3061 cctaaaacag tcaactgcat acttccacca atgattaaac ctgcttttaa atcacccatt
     3121 actaacccag catataatcc tgcaaaaact ggggcgctca ttgaagaata aatctgtaat
     3181 tcatctaaaa tttgataccc tgcatataat gttaataata atatctgcca ccattgaata
     3241 tccatgaatc attcctccta tttgattaaa agactcataa agtcttctgc tttgtcattt
     3301 ggcaccattt gagcgatcaa atgaacgcca agctgatcta atttcttgaa gacctctaca
     3361 tccgcatcta cgacattgat tgattttgtg atggaacgtg tttcatctgt ttgtgacata
     3421 ttgccgacat ttaattcttt gatcggtaca cctttttcta ctagcgctaa gatacgatct
     3481 ggttttttcg ctacgatcag cactcgttga gaatcatatt tacctgctaa aatatttgcg
     3541 gctgcttttt caataggtaa aatacttaat ttgactccag ctggcgttgc caactttaac
     3601 ccactttttt cgatcgcatt tgttgctacc tcatcgtcta cgaccatgat ccgaccaata
     3661 ttcaactttg tcgtccaaag atttgccact tgtccatgta tcaaacgtcc atcaattctt
     3721 actccaataa ttgccatcat ccattctcct tttctaactt ttttctattg gttctttgac
     3781 aaaatctata acttcactga atctaccttc tgtttcaaag ataacgattt cattttgtcc
     3841 ttttctaaat aaaccctcgg gaacaaacag tgaaagaatt ggtcctactt cccaaaaacg
     3901 accaatattc gtgccattca cccaaacgat ccctttccca aactcactaa ggtccaaata
     3961 agtatcaccg atttctgaca gttccgcttc aaagcggtaa aatgctggtt gacctgcttc
     4021 ccattttttt gaaaaatcga tgtgttctgt agtctctaaa ggtaaacaat actgtgtcca
     4081 ttctgtgata aaatgaagat cgctcatcac tcctgtacgt attccttttc tttgcgtatc
     4141 agctagcagc ttgtgtccat agttgacccg tcccatattt tcaaataata gatcaatttg
     4201 atggtcttct ttctcaagtg tcaatggaat ggtctcaccg atctctgttt ggtattgaat
     4261 cgcttgcttt tcttcatcga gaaaaacatg gacccgatca cgtccgtcga tgatccgtaa
     4321 tttttcattg accgcatctt ttttgatcgt tgttcgatac aataggtacc cagtattctg
     4381 acccaattct tccattgttt gaggataggt cgagtggatc ggcttgctga tggtatccag
     4441 tgttgcgaaa aagctgactt tttctttcaa gttgattcct tttttgacga ttgtttcttt
     4501 tatccatggt tccatttgct gcatttctgg aaaattttct gccatcattc gtttcagagc
     4561 aaaatatttt tccgttggat ttccctgctc atccaatggt gcatcgtaat cataggaagt
     4621 gacttgcggc aaatcaatcg cgcctctggc tgaacagcca ttcataaacc caaaattggt
     4681 tccgccatgg aacatataga gattgatact gccgatctgt aatgcttctt tgactgcatc
     4741 cgctaattct tccggatctc ttttaatgat cggctctttc caacgattaa accagccatc
     4801 ccaaaattcc atacacatca acggccattt tttaccatgt gcttcaaaaa actgggccat
     4861 atctgcaaaa ttttcttttg cttttgagcc aaaattccct gtcgcaaaaa catcatcgtc
     4921 aatcaaaatg cctgctctta atgtggcttg ccaaggtcca tcagaagtga atagcggtac
     4981 atcgattcca ccttcctcca agtatttttt tacttggcgc aggtatgctt tctcttcacc
     5041 atacgaaccg tactcatttt ctacctgcac cattaagacg ttgccaccat gagtgactag
     5101 atgaggaacg attttaggta gtagcgtttg gtaataccgt ttgacatact tcatgaagcg
     5161 aggatccgct gaacgaatac ggcactcctt tgtcaatagc caagcaggca agccaccaaa
     5221 ttcccattct gcacaaatat aaggggctgg acgtacgatt gcgtataatc caagggattg
     5281 cgctaactct agaaaatggg aaagatccaa tattttcgaa aaatcatagg ttccttcttc
     5341 tttttcatgg agattccaag gcacataggt ttccacagta ttaaatccaa gcgctttcaa
     5401 attgtgcaat gaccgctccc aattcgtcgg atgcactcgg aaataatgga tggcaccaga
     5461 taaaattttg aaaggttgac cgtctaacaa aaactcttct tttatttcaa atctagtcaa
     5521 gcaaatttcc tcctcgtttt tagaaaccgc tttctaattt tataatcaac gaatctttct
     5581 gacgttgatt tcccccctaa aataatcgac tttcacagga tcgatccatc ctgttttttc
     5641 ttgttgtgca ttcgccatcc cacaggccat cgcaaacgca agtgtttctt caatcgtata
     5701 gctctttgat agagcatgtg ctaagcctgc aatagtcgaa tctcctgacc ccacaggatt
     5761 caccaccgcg attttaggaa tcgtaacctg atagaagtgt ctccctactt tagcgattgc
     5821 tccttttgcg cctagcgaaa gaacgatcca attgattcct tcaaaaagcg gatgatccaa
     5881 taatgattgg atttcatcta acgaagtagc cgaagaaatt gtttggtgga ccaactctcc
     5941 taattcttcc aaattaggtt tgatcaaaaa tggtttttcc ttgccttcca agacttcaag
     6001 caaggtttga ccagaagtat ctaacaatac tcgtgttcct tttttattcg ttagggcaag
     6061 taactctgca taataacttt tagaaaatcc ttgaggcaga ctacccgaaa tcgtaaagat
     6121 atcagttgtt tccattagtc gtgcaaaatg ctcggtaaat ttatgttgct cttcttccgt
     6181 cacaacagga ccgctttcca atagttctgt ttgattcact ccatgtaaaa tagcaatcga
     6241 atctctcgtt tcccctttga taggtaaaaa ttgatgcgtt atccctgatt gatcgagttg
     6301 ttctttgata tatgctccat gctgcccgcc gactaatcca gtagctaaaa catcatctgc
     6361 taataaatga aggacacgag caacattcaa gccttttcct ccagccgttt tgttgaccgt
     6421 tttgacccga ttgacttggt cgatccgtaa ttcatccaac gtgtaagcga tgtcaattga
     6481 aggattcatg gtaactgtcg tgatcatttt gctgtcaccg aatcttcttt tactttgtca
     6541 aaccaagggg tggcgttttc atttatcact ttattcaacg attcgatatt ttgtcgccct
     6601 tgtgttctca accactcaat cgcaccttct ctaccatttt caacatatgc tttgactcca
     6661 tctttccaag tcgcacgtcc gcataacaca ccgttgaatg ttgaaccaga ttcatgagca
     6721 aagatcaatg tttgttggaa caatgcagtc gaaacccctg cactcaagaa gataaatggt
     6781 aacggtgttg catcactttg ttccttgaag aatgccgctg cttctttttt actatacgca
     6841 atttcaccat cagaataacc ttctacatag ttcatattga ctggcacttc tactttcaat
     6901 acatccactt tatatttagg atcagaaaat tctttcatca tctcgatcac tttatgcggt
     6961 ttgatcttcg catattctaa tgaagtgctg tcattggttt tggcatcata cgaaaccaat
     7021 tctagataaa acggacgctt ttcacctgca cattcactgc ctaaacgttc gataaataca
     7081 tgtttcaaat gattgatctc tgggtcttca tctacatcat aataaagcaa aaacttgatc
     7141 gcatcggctg tttgttcctt taatctaagc gtagaccaat ccgctaataa atcaggcaga
     7201 cgtcctggag ttgtcgcatc ataacccgtt ttttcatagg ctaaaagtag tcctgcttcc
     7261 tgatgtctgg ctttagcagc tggcaatcca tactctggat ctagtaaaat agcagaagca
     7321 taaggcgtta attctgctga tactaattct ttgaattcag tgatctgttg cgccgttggt
     7381 tcttttccta acgctttgat catctttttc aaagcaccgc gttgatcaat tgccaatgca
     7441 ctgatgattc cttcttttgt tgataaactc ttcatagctg caattttttc ttcattcatc
     7501 cttcgtttcc cctttgttca catattcatg gataatgaca cctttgacca ctcgattgac
     7561 tgtacctgta ggcgatggtg tatctggttt attcgctact tttatcgacg tatcgagcgc
     7621 aatgatttgg gctgtcatga tcataggtaa ggcaagatac gcatcaggta ggctgatcga
     7681 agcttctaac aaaagacttg tacctgtgaa atcgatgttt cctggttggg cgatcgcgat
     7741 gacactcgtg gtaatttggt catcttttat ttcttctaga atatctaagt cataatttct
     7801 tgtatacggt tggttattaa caaaaccaat aacaagtgtt tcgctattta aaaacgattt
     7861 tggtccatgg cggaagccca tagaagaatc aaagatcgtt gccactttac cggctgtcaa
     7921 ttccaatatt ttcaattgtg cttctctcgt tgcaccagcc aaacttcctg aaccaagata
     7981 ggcgatacgt tcaaatgact taggcaataa tttttggatt tcttgatctc gtgaaattag
     8041 atcttctcca gttctgatca attgttgaag ataactttct tttttctcaa cggcagtttg
     8101 gtcgaatacc cataacgcac tcaacaacat gcacgtaaaa cttcctgtca tggcaaatcc
     8161 tgcatcattt gatttttctg gcatcagtaa aagataattt cgttcatctg tcgctgcatt
     8221 ctttgctaac tgaccttctt tagcacaagt aatcgtcaaa tgaaacaatg tcttgatttg
     8281 ttcattggca atcttgactg ttgccacact ttctggacta tttccactac gtgcaaatga
     8341 caccagtaat gtcggttcat ccggaaatag aaattctttt ggtgaagaca cgatatctgt
     8401 cgtaccaaag ctatgaaaaa taaagtgatc gttatcaccg tattcattta aataaggtac
     8461 tgctgtatcc ccgacatatt gtgatgtgcc tgcaccagta aaaatcactc gaactctttt
     8521 gccatttgct gcttttttta tctggtcaaa aaacaatgct aatttttctt gattctgtgc
     8581 taatatctca gctgtttctt tccataattg cggttgctgt ttgatttctc gcgtagttat
     8641 ttccgcaccc aactctttta atttatcctt cgatagttca aacatgttgt tttccctctc
     8701 aatcattttc ggacgtggcg gatcttataa tgaaactggt ctgctctcgc tacactatcc
     8761 gtatattcaa taatttcatt tttcacatta taggtcgttc ggcttagata taataccggg
     8821 gcaccatcag cgatacgaag cggatcagca tctttctttt gaacgatact tgcataaaat
     8881 tcttcatctg ctaattggac ttgctgttca taatcctgag aaaacacatc atacaatggc
     8941 ttactttcaa gtaatggcag atcaagacca ataaatagtt ttgcaggtaa ataagaacgt
     9001 tccaccatca atggttcgtc atccgctaaa cgaagtcttt tcaatttgaa aactgtttcg
     9061 cctaaattga tgtttagatt cttggcaacc tttttgtttg cttccatttg cttgaattcc
     9121 aagatgactg tattaggcac ccgtcccaat tccctcatct cttctgtaaa actatacgct
     9181 cccgccagat cagacgactt atctgataga tcagacacat atgttccttt tccgtgtcgg
     9241 cgataaatat acccttgcgt ctctaattct tgcaaagcaa ggcgaaccgt tgtccgactc
     9301 actccataat gctgacaaag ttctctttca gataacaatt tgtcatgagg tgtcatctgt
     9361 gtttcgatac gttctttcaa tacgtcaact agttgataat ataatgattt tcctttactt
     9421 gagctctcca taaaaatacc tcttctttac tggttataac cacaacacaa gaatacccac
     9481 atattcctca tttgtcaaag ggtttttggc gtaaaaaagc tattataaca aggtttcaag
     9541 gtaatttaga actggtatca aaagatacct caaaaatacc agttgacaaa taaaaaacaa
     9601 aaaaaagctg aatcttggca aagattcagc taaatggtct agagaagtaa agaaaaaata
     9661 ttctttatac atctgtggct atttctacta tcgacaagca aatgggccta atagactact
     9721 atgtaaataa aaatggcttt ctctaaataa agtaggattt tttatttttt agataacttt
     9781 tctctcatgc cggtctgata gtcgaaattt tgttgtacat ttttaaaaat aacaaaaatt
     9841 ttttatgata attctgtctt tttctagttt ccttatggcg taagtttaac gaataagaat
     9901 ttagtatcta tacttttata ttacaaaaaa tattcgtaaa attcgggctc tatacttttc
     9961 gatgtttgtg aacttta
//
LOCUS       DAXWLB010000107         9819 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXWLB010000107 DAXWLB010000000
VERSION     DAXWLB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.107"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(97..957)
                     /locus_tag="ACGE4H_001991"
     CDS             complement(97..957)
                     /locus_tag="ACGE4H_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFF8365394.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(1016..1648)
                     /locus_tag="ACGE4H_001992"
                     /pseudo
     CDS             complement(1016..1648)
                     /locus_tag="ACGE4H_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            1815..2576
                     /locus_tag="ACGE4H_001993"
     CDS             1815..2576
                     /locus_tag="ACGE4H_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8365395.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            2602..5193
                     /gene="mprF"
                     /locus_tag="ACGE4H_001994"
     CDS             2602..5193
                     /gene="mprF"
                     /locus_tag="ACGE4H_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFF8365396.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(5381..6568)
                     /gene="tuf"
                     /locus_tag="ACGE4H_001995"
     CDS             complement(5381..6568)
                     /gene="tuf"
                     /locus_tag="ACGE4H_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFF8365397.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            6936..7292
                     /locus_tag="ACGE4H_001996"
     CDS             6936..7292
                     /locus_tag="ACGE4H_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8365398.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(7533..8537)
                     /locus_tag="ACGE4H_001997"
     CDS             complement(7533..8537)
                     /locus_tag="ACGE4H_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFF8365399.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            8703..9554
                     /locus_tag="ACGE4H_001998"
     CDS             8703..9554
                     /locus_tag="ACGE4H_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFF8365400.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            9571..9816
                     /locus_tag="ACGE4H_001999"
     CDS             9571..9816
                     /locus_tag="ACGE4H_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365401.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         2934 a         1952 c         1750 g         3183 t
ORIGIN      
        1 taaaatttat agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg
       61 aatcacccgt gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg
      121 gctcatccac caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc
      181 agcaactccc aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac
      241 acctaaaatg aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt
      301 tgacaatcca agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg
      361 tacttcttta aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga
      421 agggctcaat cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt
      481 tccaaaattc tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata
      541 agccagaaca aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc
      601 tgcttctttc cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat
      661 cgttgtttca tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt
      721 cactccgcct agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag
      781 gtgggctaaa aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc
      841 gctgactcct ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat
      901 ccagtctttg gttgtgtacc ccacttcttg attttccatt ttttgccttt tttccactat
      961 ttgcgcactc cttctttcat ttaattttat tcatgattga acatttcctg ctattttact
     1021 taaagacttt ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa
     1081 acatgttacc tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc
     1141 aatatcggta ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat
     1201 acgcttctgc taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat
     1261 ctttcccaaa attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt
     1321 ctactatcat ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa
     1381 tgatctcagc ttctccatgc agactttccg agctctgcat gagatctgtc ccttttcctt
     1441 ttgattcggt caatattatc gccttgatca aattctcgta gttttccata tgataatttt
     1501 ttgcagctgt tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc
     1561 ttcctcataa aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac
     1621 ggataataga taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt
     1681 atcttactat ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag
     1741 aaaaaatgaa ttttcctgta ttttttgaca tcttacgaaa atgatcgttg tttttcagta
     1801 aaaacaattg cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga
     1861 aaaaagaaaa gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg
     1921 gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag
     1981 aacaggtccc tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg
     2041 gtcgttcgac aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata
     2101 cgatcatgtt gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa
     2161 atctagcact ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt
     2221 ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt
     2281 atgcatgggt atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta
     2341 tcaaactttt gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc
     2401 ccactttgat catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag
     2461 ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta
     2521 gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat
     2581 taggaataga aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta
     2641 ggactgttca aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg
     2701 tccatcggta aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta
     2761 tggaaaagcg gactaatgct attgattggt cttatctctg tgttacctat ggttggttat
     2821 gatattattt tgaatcggat gcttgaacaa aagcagaatc ctcgttacct gtttgagaca
     2881 agctggctga tcaatacgat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg
     2941 cttcgctctg aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg
     3001 aaagttttct tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt
     3061 gtagtcttca cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc
     3121 ggtctatatt ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt
     3181 ggtacctcac ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt
     3241 gtcctgttta gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa
     3301 acaattccat tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa
     3361 attgggagtt tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa
     3421 atagtcgttg tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt
     3481 ggaatcgtct ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc
     3541 cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct
     3601 ggaatcatgc tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt
     3661 catagtttga acccattgaa atttcatttt attatccaat ttcctagtat actattggga
     3721 tttcttttaa tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct
     3781 acaatcttct tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca
     3841 ccggtggttt tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt
     3901 cgggaacaat tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca
     3961 ctgtccttgc tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt
     4021 caccatttta tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg
     4081 gccatcatcg ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag
     4141 aaacagattg gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga
     4201 ggaaactccg atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa
     4261 gacggtgaac caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg
     4321 ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa
     4381 acagatcgac tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt
     4441 ttgcatgaat tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat
     4501 agttttacaa cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa
     4561 cgagaaggat ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact
     4621 ttcaaaaata tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc
     4681 tttttctctg aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa
     4741 gaaattgtcg cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt
     4801 gatctgatgc gatatgattc taaaaaagca ccttctggca gtatggattt cttgttcttg
     4861 catttgtttg cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct
     4921 ctttcaaatg tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt
     4981 tacgagtttg gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac
     5041 gctacaaact gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt
     5101 atgattgctt tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt
     5161 ggtttgaaaa aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc
     5221 tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt
     5281 atacaattga agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt
     5341 ttaaacattt ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa
     5401 cgatacctga acctactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa
     5461 cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt
     5521 ctgtatcttc tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaactgtg
     5581 gacgatagtt gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca
     5641 cttctgcttt gaattttgta tgtggagtaa tggaacctgg tttagcaagt acttgtccac
     5701 gttcgatatc gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc
     5761 cgtaatctaa cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag
     5821 gtttgatccc tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac
     5881 ctgaggcaac agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg
     5941 gtttatctgt atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca
     6001 taatagcagc ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca
     6061 caggagtgtc atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta
     6121 ccaaatcgat caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt
     6181 attttacacc aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt
     6241 cagttgcaga aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt
     6301 tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat
     6361 attctacgtg tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa
     6421 tactagcata gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag
     6481 cagttaaagt agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt
     6541 tacttctgtc ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac
     6601 tgaaaaattt cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat
     6661 acgtccgact tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa
     6721 agtcaactaa gagcactcgt ctgtcttata cgcgaaaaaa gtacctttta taaataaaaa
     6781 aatgtgtttt ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa
     6841 caaaatgagg aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt
     6901 ctatttcgtt aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg
     6961 tcgttttagg catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg
     7021 aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg
     7081 gagagaaatt cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa
     7141 aacaggtgac gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac
     7201 tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc
     7261 ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa
     7321 aaagaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
     7381 gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt ctcacgctct
     7441 ttttttgtta tattcgtctt ttccgatcct tctcttgaca caattcattt aactgatcac
     7501 tggtacttga acacctaata ttctagtcat ttttataaac gattcaatac ttcatccaca
     7561 tttttgatca tcacggatat cgtaccatcc ggattattca caaattcaat caaatctggg
     7621 ttgcgatata catctactgg tacgatcagt tcgatcccgt ttgatagctt gaatttttgt
     7681 tttccaaatt ttttctcaga aatttcttgt acttctttta caggaggtgc ttctgggaca
     7741 aatccttgct cttgtacttc ttctttaaaa gccatttttg ccgtgatgtt ttccttgaag
     7801 actttttccg caatcatctc atgatccaac cgaccttttt cttctatcgt atcatagaca
     7861 gcttctttca aatcagccat gatatcaaat tcttctgtct caaatttttt tccaagatgc
     7921 ttggctactt tttggatcac ttttacattt tcttctaaag aaggtgcagg aacactttcg
     7981 atcactcttc cagaaaaata aagctctttt ttgccagaaa actcatattt tttttcaatt
     8041 aattcgtagg tccaatcagt caaattcaca gcgaatgctt catctgcttt ttgtgttttc
     8101 gcccctagaa ttgctcgatt caaaatcaag cggttgtcga tccctgcttc gtctgcttca
     8161 acaaagtgag tgtaagcctc acgataattg actttcaata aagataagta catcactgta
     8221 tctaattcat acaagacaac aaatacatct gcactaggtg cttcttcact ttcttggtag
     8281 atatcgaacc accgctgaac gattgcttca cttgcttcca caaaattttc ttgagcgatt
     8341 ttaagtgatt caacgatcat gctttcttct gctagttgtc cagtttttgt ttgagcagaa
     8401 gagatttttt ggatcttctt ttgaagaaaa tcacgaatat attcttttgt cagatccagc
     8461 tctttttgtg aaaagactgg agaaccagtt tctctgtcaa taatatgcag aattgctttt
     8521 tttaaataga tatccatttt ctcattccct tctgtcttag tttacggaaa aaaaatcaaa
     8581 aataccagaa tcaaaatcat tttcttttaa acaaaaattt caagagtaga aacaaaatgg
     8641 attctttttt tcgaaaaatg cgctacactt atagttgaac gaataaaaaa ggagctgaaa
     8701 gtatgaattc attaacaagc acttatcgtc tatcaaatgg ttacgaaatc ccggtagtag
     8761 gattcggcac atggcaaaca cctgatgggg atgtcgctgt ttcctctgtt aaagaagcgc
     8821 tggctgcagg ttatcgccat atcgatactg cccaaggata caagaatgaa gaaagcgtcg
     8881 gccaagcgat taaggaaagt ggaattccta gagaagagat attcttgaca acaaaattat
     8941 ggaatgcaaa ccacagctat gaacttgtga tgtcttcttt tgaagaatca ttgaagaagc
     9001 tccaaacaga ttatttagat ctgtttttga tccactggcc aaaccctgtt gcttttcgtg
     9061 ataattggga acaagcaaat gcagatacat ggcgagcatt tgaagaactg tatgaagcag
     9121 gtaaaatcaa agcaatcggt gtgagcaatt tcttacccca ccatctagat acactagcaa
     9181 aaacagcaaa aattatgccg atggtcaatc aagttttcct tgcaccaggc gaattacaga
     9241 caactgtagt tgagtatgca aaaaaacatg atatgatcct cgaagcatat agtccattag
     9301 gcactggtaa aatcttcgat gtgcctgaaa tgaaacaaat cgctgaagca catgacaaga
     9361 cgattgcaca agttgctttg cgttggtcgc tgcaacacga attccttcct cttcctaagt
     9421 cagtgacacc aagcagaatc aaagaaaata ctgaattatt tgattttgaa ttaacagaag
     9481 aagaaatgaa acaaatcgat cagttagatg gtgtagtcgg caaagcaaaa gacccagata
     9541 ctacccaatt ttaagtcgaa aggtgatttt atggatccaa tcgaacgctt gaattctctc
     9601 tcggaagaag ttacccagac ttttcattcc gattttgtct ttttgatcga tgcagagaaa
     9661 atccaacatt ttcctgcaag aaactggaca catgatcaaa tcatcgaaga actgaaaaaa
     9721 cggttcgatc attcgctaat ggtcaaaccc tggcacgaac atgaagtcat ttattcacct
     9781 gaactacctg tttttgcctt gataccgaaa aaatagaaa
//
LOCUS       DAXWLB010000108         9803 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXWLB010000108 DAXWLB010000000
VERSION     DAXWLB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.108"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(120..1004)
                     /locus_tag="ACGE4H_002000"
     CDS             complement(120..1004)
                     /locus_tag="ACGE4H_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8365402.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(1091..1990)
                     /locus_tag="ACGE4H_002001"
     CDS             complement(1091..1990)
                     /locus_tag="ACGE4H_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFF8365403.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(2007..2513)
                     /locus_tag="ACGE4H_002002"
     CDS             complement(2007..2513)
                     /locus_tag="ACGE4H_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFF8365404.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(2702..3322)
                     /gene="rpoE"
                     /locus_tag="ACGE4H_002003"
     CDS             complement(2702..3322)
                     /gene="rpoE"
                     /locus_tag="ACGE4H_002003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFF8365405.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(3398..3835)
                     /locus_tag="ACGE4H_002004"
     CDS             complement(3398..3835)
                     /locus_tag="ACGE4H_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFF8365406.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3998..4828
                     /locus_tag="ACGE4H_002005"
     CDS             3998..4828
                     /locus_tag="ACGE4H_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFF8365407.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            4812..6179
                     /locus_tag="ACGE4H_002006"
     CDS             4812..6179
                     /locus_tag="ACGE4H_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFF8365408.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            6198..7007
                     /gene="yidA"
                     /locus_tag="ACGE4H_002007"
     CDS             6198..7007
                     /gene="yidA"
                     /locus_tag="ACGE4H_002007"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFF8365409.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(7050..7493)
                     /locus_tag="ACGE4H_002008"
     CDS             complement(7050..7493)
                     /locus_tag="ACGE4H_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFF8365410.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            7627..7998
                     /locus_tag="ACGE4H_002009"
     CDS             7627..7998
                     /locus_tag="ACGE4H_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8365411.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(8167..8949)
                     /locus_tag="ACGE4H_002010"
     CDS             complement(8167..8949)
                     /locus_tag="ACGE4H_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFF8365412.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(8946..>9803)
                     /locus_tag="ACGE4H_002011"
     CDS             complement(8946..>9803)
                     /locus_tag="ACGE4H_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFF8365413.1"
                     /translation="RLVEILPDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHL
                     PSHLGEIFLFDAQNGLPKALINANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQ
                     ALSHLTAISTIRPITTVFVYDILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEI
                     ICTLTSSKEAFLQKEWVREDAHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTES
                     GDLLIAMAEGDLEESCIVGTLGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIY
                     NKLEKGALK"
BASE COUNT         2837 a         2010 c         1744 g         3212 t
ORIGIN      
        1 aaattggtgg acagtttttt gttgtacctg tatagatatt gttaagcctt tgcaagtaag
       61 gtagttatgt gttcaactac ttattaaaat gtgttattta ttaaagatac ttgtctcact
      121 tactgaactg gccgatgatc gtttctgcca taatagttct cgacagccgt tggatgccct
      181 aataaatccc caactgtcgt gatgcgatac ccttgctctt tcagataatc gatcacttga
      241 ggaacggcac gaatcgtttc aggatagata tcatggaata aaataattgc cccatcgtat
      301 actgttgctt ggatcttctg caaaatcaaa tcaggatttc ttgtcttcca atcttctgaa
      361 tcaacagacc attcgatgat cggtcgctgg atgatcgttg ctcctacatt cgtgacagaa
      421 ccatagggcg gacgaacaaa agtgggggtt ttgccagtga cttttttgat ctcagtgtct
      481 gctttcgtca cttcctccgt catcgactgg gcagaaagag tggccagatt ctgatgatcg
      541 tatgtgtgat tgccaatctc gtgccctgct tcagctgtct gcttcacgat ttcaggatgc
      601 tgcgccgtat tttctcctaa gacgaaaaaa gtggcatgca cgtttttttc ttttaagatt
      661 cgcagaagct gcggtgttgt ggtcgctcct ggtccatcat caaagctgat tgcgacgctt
      721 ttgacagccg gatctcggct cgtttttgta ctgtcttgtg ttgattgtga tgactgactt
      781 tgtacctttt ccgctgattc ttgaatggct tctttgacgt tagctgtttc agttgattgg
      841 cttttaatgg ttgattgttc tgattgtttg aaagattgtg acgaatgctc cgaagagtgt
      901 gtttgtcctt ttacttcggt gatcaaggag gccaccgaag taatttcgaa aaacaaaagc
      961 acggataaag caataatact gataactaaa taatttcttt tcactatatc tcttctttct
     1021 ctttttctct tcttattatt tacttgtact tacttgaaaa ttgaaactgc tagtcgcaac
     1081 tacttctcgt ctaatgatcc aaataatctt ccgatgccaa aatcgttttt gtcacggact
     1141 taggaatgac aaattctggt gtacccatat aaccaggagc aatttcgtat tgagaaaaag
     1201 tcaaaactaa ttgatgctct ttgttcaaat aaaaacttcg tgtaggcgta acaagtgttg
     1261 gtttttctat cgtcccttct tgaatatctt gctcagtcca atagtactta gatggatctt
     1321 ccttgacttg ttcggccatc tgccgtttga cttcttttgt caaaacagat agataggtgt
     1381 catctttgaa taaaagtgga agtgagagct ggacactgtt tttcttgtcg attgtgtcgt
     1441 atcttttttc aacatggcta tctgctgctg tttgggtgat ctgcctttca atcacgagaa
     1501 accggttatc gtctacgatt ttctgataat ctcccaaaac ttggtaatga tccgtttcaa
     1561 aatttccata ttctttttca gctttttcaa aatctgcttg tccttcgcga aagtattttt
     1621 gatttaatcc atgaattgtt ttattttctt tctcattttg tgtattgatc ttcggtacct
     1681 gaatatcgat tttttcagat cgatcagtaa agcgttcgcc taagatcaat tcaatgactg
     1741 atcctaagat gggtaaatct tctgcaaagc ttcgaacttg actattaaaa agaaggctac
     1801 ttgttgtaat cattacgata gccaaagata ctccgaaccc ttggagacgt ctctttctcc
     1861 gctttgcttt tataaatgat tgctcttcga taataaaacg ctggcggatc tcctctttaa
     1921 gccctttggg aattgcttgt tctttataag ttttctttag tttttttaaa ttttcttttt
     1981 gggaatacat ggcactcttc tctccttcat tctaggattt gacgtaaagc attcaatgag
     2041 cggtacattt tcgttttaac tgtgttttca ttcatatcca agatagtcgc aatttctttc
     2101 aaacgaaatc cttcaaaaaa gtagaggatc agaatctctt tttccggact gtctatttta
     2161 tctaaaatca tatggagctc tgcaacggaa gtttctaaag gtaactgttg ggggatatct
     2221 tgaatttcaa ataagtccac aggcgtcctt ttttgtctgc gccaactatc taatgccgtg
     2281 cggatcaata tccgataaaa ccatgcacta aaatgctcga tctctttttc tttttcaaaa
     2341 tagttcaggg ctttatgaaa actatcttgc atcacatcta aggcctcttc tttatcatgt
     2401 acgtaattca tcgtgatacg gaacattttt tgataatcag tttcgatgag tccattcagc
     2461 aggtgacggt atttttcgat ctgccgtttt ttcttatatc cagtaaaaat cattctccac
     2521 tcctttctgc ctcacttaat atgacgtaca aggtgcgcta aaagtttcaa tttttttatt
     2581 ttttatgtat ttacttggga tttttatata caaaaaagaa ggtgtgacaa aaatcgtgtc
     2641 acacctcctt attctgatag gctgatcgtc tctgtcacaa cctctcgtaa gaaaataatg
     2701 tttattcttt ttcagagtca gaaaattctt cctcttcata atcttcatca tcatcgtctt
     2761 cgtctatgat acttaaatct tcttcgatat ttcctggcaa gtcttcttca tcatctgtcg
     2821 ggtcatctgc tccgatttct tgcaagtcag aattataagc agcgatttct tcatcttcat
     2881 catcatcgtc atcaaacaga tcatcgtcat cttcttctgt cagatctgta tcttctgggt
     2941 catcgtcatt gtaatcgatc gcgtcttcat catcattgat aaaggcgttg actttcttgc
     3001 gtttacggcg acgtggagta tcttcatcct cttcgtccat tccatggttt acttcttcat
     3061 cgatcgaatc gatagcatac catgaacgca agccccaacg attatcccct aatgagataa
     3121 agctgccgtc aatattcaag tcagtataaa attgtgcgag tgaatcacga atttcactgt
     3181 ctgatttccc aagatagttt tggatctggt tagctaaatc agaaaaatcc atgacatcgc
     3241 cacgttgttc taagatcgcg tgtgctactt cgatcattga taattcgctt ttgtttgcac
     3301 cttcaaatac actaatttcc aatcaggaca cgtcctttca cagtctactc cttatcatac
     3361 aaaatcagac acaaaaaatc aaacttttct tttatattta cgctgtaaaa tccaaggtca
     3421 aataatattc gccgattttt tccggtccca tgaacagatc ataatcgatt cgaaccttcc
     3481 ctgctgtggg acggtctttc aaactaacat gcagttcttt cgtaaaagta gcaatctgga
     3541 tcataccgta tggtgttcga taggatgttt ctaaccgttc tttatagcca aatttcaaac
     3601 gcatccgcat ttctcctgca cggatcagct gtacatgacc atctggaact aatttgatcg
     3661 ttacaggaat ctcatcgccc gtctcctcct gaatctcttt ataacgtatg tacagcgtat
     3721 cgccaattgt caccatttgc cctttcaaat caaaataaaa atcttgcact tccccttgct
     3781 gttttacttt tgtacgcaat ttgattgaaa tcggtgttcc ttgtgataat tccattattc
     3841 attctccttt acttctcata gctacattgt tcctagtttc ttttgttttg tcaaccaact
     3901 tgtccatttt tacagaaaac cttttttcca aaacaactct ttgattttag agtataatat
     3961 tgtagaaaat atgaatttat acaaaggagc cggttgaatg aaccctgcga ttcgactaga
     4021 tcagcacatt tatcaaaaaa acgacttttc cccttttgca ttaacagata tattaacgga
     4081 atattctaaa gaacaccagc aaatcttttt acatttctgg caatatgact gcacagtcat
     4141 cttaggtatg aaggatgttc gaactccttt tctaagtgac gggatccaaa cactggaaga
     4201 tgcaggttac gctccagtga tcagaaattc aggtggatta gggatcgtat cagatgaagg
     4261 aattttgaat atttctttga ttttccccca aagtgacaca aaaaaaataa ccatagatga
     4321 cgcttacgag caaatgctcc tattgactaa atacgctttt ccaaaggcac cgatacaagc
     4381 attcgaaatc acccattctt attgtcctgg aacttatgat ttaagcatcc atggcaaaaa
     4441 atttgccggt atcgctcagc gtcggatcaa aaatgggatt tctgttatga tgtacttaag
     4501 tgtcaacggt aatcaggaaa aacgtgggca agtcatgaag cgattttatc aatctgcttt
     4561 aaaagaagac tttggtacaa atggctatcc tgcagtggat cctgctacaa tggcaacact
     4621 tgaagaactg cttgaccaac ccctttttgt aaaagaagtc aaagaagcat ttgcaaaagc
     4681 ttttgaaaag ctgtattctg caagtgattt acaggattct accgactggc taaaaaaaga
     4741 aatccagctg gagcaatgga acaaccaaat ccagagaatg gaacaacgta ataacataaa
     4801 ggagctacat tatgacaacc ccttataaat atcaaatgct gcctatggaa aaagtttttc
     4861 gtgatccagt ccataactat atccatgtcc agcatcaagt aatcttagat ttgatcaatt
     4921 cgaaagaagt ccaacgtcta cgacgcatta aacaattagg cacttcttct ttcacttttc
     4981 acggagcaga acatagccgt tttactcatt cattaggcgt ttatgagatt tcaagacgaa
     5041 tctgcgatat cttttctcga aatttttcaa aagaaaaaat aggcaacgga ggctgggatg
     5101 atcaggaacg gctggtgact ttatgtgcgg cactgttgca tgatgtcggt catggtcctt
     5161 actctcatac atttgaacat atcttccata cgaatcatga agccatcact gtagagatca
     5221 tcacttctcc agaaacagaa gtgtatcaga ttcttaaccg agtagaagaa ggttttcctg
     5281 aaaaggtagc tagtgtgatc caaaaaactt accccaaccc acaagttgtc caaatgattt
     5341 ctagtcagat cgatgcagat cgaatggact atttgcttcg ggatgcttat ttcactggaa
     5401 cagaatatgg aacatttgat cttacccgaa tattacgtgt gattcgtcct tatgaaggcg
     5461 ggatcgcttt ttccatgagt ggcatgcatg cagtggaaga ttatatcgtt agccgatatc
     5521 aaatgtatgt gcaagtgtac ttccatcctg tttcccgtgg tatggaagta attttggagc
     5581 atctgcttca tcgcgcacac gagttgtatc aagaagatgc cagcagtttt gaactccatt
     5641 ctcaattact tctgccattt cttgacggaa gctttacttt agaagaatac ctcaaacttg
     5701 atgatggggt cttgggcact tactttactc aatggtctga atctgctgat cctattttaa
     5761 atgatttagc taaacgtttt ttgaatcgta aaccactgaa atctgctact ttttctggca
     5821 accgggattc aaaattagtc caagaactca ctttgttagt agaaaaggtc ggatataatc
     5881 ctgtttatta cactgctgtg aattctagct acgatctacc ttatgatttt tatcgcccag
     5941 aacaaggcag acatcgcaca caaatcgaga ttttgcgaaa tgacggaact ttgatagaat
     6001 tatcacaggt tagtcagctt gttgctgcat tagcgggaca agaacaagga gatgagcgat
     6061 ttttctttcc aaaagaaatg gttgatccgt ccttgcgtga tcattatgat ttatttgacg
     6121 aaacttatca agaatttgcc agccatatcc gtaatggcgc attgattgaa ataaattaaa
     6181 atatattgga ggaaattatg tctatcaaac tagtcgctat cgacatcgat ggtactttat
     6241 taaatgagaa aagagaagta acaaaagaag taaaagaagc gattgccgct gctgttgcaa
     6301 aaggtgtttc gatcgtttta tgcactggcc gtcccttacc aggtgtccag gaacagttaa
     6361 acgagttgaa cctttttcaa gataatgatt acgttattac ttataacggg gcactcgtac
     6421 aacaaacgaa atcaggtaaa atcatcgccc gtcacggact gacacatgaa gatttcctag
     6481 agatcgaagt aatggcacgc cgtgtcggca gtcatctcca ttcgatcgat gacaaagcga
     6541 tctacacgcc taatcgagat atcagcgctt atacgatcca cgaagcaagt ttagtcaaga
     6601 tgccgctaaa atatcgaaca cctgaagaaa tgacaccaga catgaacatc gtcaaaatga
     6661 tgatgatcga cgaaccagaa attttagatg cagcaattgc gcgtttaccg caaactttcc
     6721 gtgataaata cacaacggtc aaaagtgcgc cctattactt cgaagtacta aataaagaag
     6781 caagtaaagg tgcggcagtt gcgaatctcg cacagcacct tggtattgat caagatgaaa
     6841 tcatggctat cggagataac gagaacgact tatccatgat tgaatatgct ggtctaggag
     6901 tagcaatggg aaatgccgtt tctcttgtca aagaagcagc aaatgtcatc acttcatcaa
     6961 acgacgagca cggtgtagct gaagcaatca aaaaatatgt actgtaataa ataaaaaaac
     7021 tgagagaaag ctgatctact cgctttcttt cagtttttat ttttttcgat ccatctctcg
     7081 actgcccatc ggtggctacc acgtttcaat ttcacaagcg cttcttgagg gctcacccac
     7141 caaattttat tggtcttttc agttggttct cctatacgct tccaagaatc agcaacaaag
     7201 aaatatcctg gattataata ataagttgca cgataattcg agtaaaaata ctcatcagct
     7261 tctcccagat aagaagaaat ttctgccaca attcccattt cttctatcag ctcacggaca
     7321 atcgcttctt ctttcgtctc ttctccttca atttccccgc caggaagaaa aaaagcacca
     7381 tttggagcct cgattactgc aatctgctga tctctttcta tgacaatata tgctgcataa
     7441 cgggtttgat aatcagcaaa ttgctttttt tcaccaaaaa ttggtttatc catgaatagt
     7501 ttccacctta acagttttat tttctatcca ttttacatga gaaatttctc atgaacaagc
     7561 acaaatcaac tatttcctta cttctttttg ttatgattga gtaaaaggaa aaggagagat
     7621 gaatctatga aaatggctca cacatgtgtt cgagtgaaag atcttgaagc atcaatcgaa
     7681 ttttatcaaa aggcatttgg ttttgaagaa agccgccgcc gtgattttcc agaaaacaaa
     7741 tttacacttg tttatttgac acttcctggg gatgactatg aattagagtt gacttacaac
     7801 tatgatcatg gtgcgtatga tctaggagac ggctatggtc atatcgctat agcagctgat
     7861 gatctagaaa aattacacga gcaacataaa gctgctggtc ttgagattac tgacttaaaa
     7921 ggattacctg gcactgcgcc ttcttactac tttgtcattg accctgatgg ttataaaatc
     7981 gaagtcatcc gaggatagtt agtatggggc attttactct tgattgaata aaaaacaaac
     8041 accaataatt aaaatgagac gcaaactttt cattaaattg aaaaagtttg cgtctttttt
     8101 taacaaccat tgtagctata aggttgccac tctccaccaa tttattttta attcatttga
     8161 aagaaatcaa ataaacgagg tacctttaga aaacaaaact gcttttcctc ctattatcac
     8221 acgatcttct tttaattcac attctagatc cccgcccctt gatgacattt gataagctga
     8281 caattttgtt ttattcattc tttctgccca ataaggaata agattagaat gtgctgatcc
     8341 acatactgga tcttcattga ttgttaattt tggaaagaag gttctagaaa caaagtcttt
     8401 ttgcacactt tctgctgtca cgatgactcc tattcctaat tcaaaatttt tgatagctgt
     8461 aaaatctggc tgcaatttag ccaccgtttt ttcatcttct aatacgaaaa ataaatcacg
     8521 tgctaagtag gcttctttaa tcttagcccc aatcgcttct tcatattcag acaatatcgg
     8581 cacttttttc ggcataatac taggaaaatc catgtaataa taatctttaa ttcttgtcac
     8641 aaatagatta ccgctttgag tagaaaattt aatcgtttca tcaggtattt tgtaatagtt
     8701 aaataaaaca aatgctgttg ctaatgtcgc gtgaccacaa agatcgattt ctcgatctgg
     8761 cgtaaaccat ctaagctcaa attcttggtt tttcttcact gtaaatgctg tttcagataa
     8821 attgttttca atggcaattt tttgcatcgt accttctggc agccattctt ctagcacata
     8881 aaccgctgct ggatttcctt tgaacacctc atctgcaaat gcatcaacta cgtaataatc
     8941 taagttcatt ttaaagcccc tttctctaat ttgttataaa tatattctgc acaacaaaga
     9001 tcttcaatag ctagaccaac tgcgtcgaaa atcgtcggcc tcgattctcc tttataaaat
     9061 gtttttttct cagaaactaa ttcgcctaat gtaccaacaa tacaagattc ctctaaatct
     9121 ccttctgcca tggctataag caggtccccg ctttctgtta aggcagctga gtaatcatct
     9181 acatagattt gagaagtttc catcaacgat gtacttatct ctcttgtatc aggagtaaat
     9241 gtgcccacag cattgatatg tgcgtcttcc cttacccact ctttttgaag aaatgcctct
     9301 ttactagagg tcaacgtaca aatgatttcc gctggacgta ctgcatcttc tactgatatt
     9361 gcatttataa acgttatttt aggatactgt tttttagctt tagcgataaa agtatttcgt
     9421 ctttcttcta aaatatcgta aacaaacact gtagtgatgg gacgaatggt agaaatcgca
     9481 gtcaaatgcg acaatgcttg ttgaccagct ccaattaaag caagtgtttc tgcatgcgga
     9541 tttgctaagt aatctgttgc cagcgcagat acagcagctg ttcgaatcca ggtgactgca
     9601 tttgcgttta tcaatgcttt gggtagtccg ttttgagcat caaacaagaa aatctctcct
     9661 aaatgagaag gtaaatgata ctgatgatta tccggaaaag cggtaatgac ttttgtgcca
     9721 aaatatctgt tctcccccaa ataagcaggc atcatcgcaa acatattttt gccttttccg
     9781 tcaggtaaaa tttccaccaa tcg
//
LOCUS       DAXWLB010000109         9360 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXWLB010000109 DAXWLB010000000
VERSION     DAXWLB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.109"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1501)
                     /locus_tag="ACGE4H_002012"
     CDS             complement(<1..1501)
                     /locus_tag="ACGE4H_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365414.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFT"
     gene            complement(1599..1874)
                     /locus_tag="ACGE4H_002013"
     CDS             complement(1599..1874)
                     /locus_tag="ACGE4H_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365415.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            2106..2840
                     /locus_tag="ACGE4H_002014"
     CDS             2106..2840
                     /locus_tag="ACGE4H_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8365416.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2879..4021
                     /locus_tag="ACGE4H_002015"
     CDS             2879..4021
                     /locus_tag="ACGE4H_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8365417.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(4116..5507)
                     /gene="sufB"
                     /locus_tag="ACGE4H_002016"
     CDS             complement(4116..5507)
                     /gene="sufB"
                     /locus_tag="ACGE4H_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFF8365418.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5512..5982)
                     /gene="sufU"
                     /locus_tag="ACGE4H_002017"
     CDS             complement(5512..5982)
                     /gene="sufU"
                     /locus_tag="ACGE4H_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFF8365419.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(5969..7204)
                     /locus_tag="ACGE4H_002018"
     CDS             complement(5969..7204)
                     /locus_tag="ACGE4H_002018"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFF8365420.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(7201..8487)
                     /gene="sufD"
                     /locus_tag="ACGE4H_002019"
     CDS             complement(7201..8487)
                     /gene="sufD"
                     /locus_tag="ACGE4H_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFF8365421.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(8500..9273)
                     /gene="sufC"
                     /locus_tag="ACGE4H_002020"
     CDS             complement(8500..9273)
                     /gene="sufC"
                     /locus_tag="ACGE4H_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFF8365422.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         2747 a         1900 c         1659 g         3054 t
ORIGIN      
        1 atgtaaaatg aattttttcc agaagtggaa ggatatcttc attcaatttt tgaatcacag
       61 aagttgcatt aaaaacgtct aatcgataga cattgcttct cttatgatta aagtttccta
      121 acgctttctc ttcctttaac ctatttaggt attcgagcat cttcaaagga acatcttcat
      181 catctaattt ttttatcaat cctataaaag tcataagtga tagtaactta ttacatttat
      241 ctatgtcata agttttacca tagtaagaac tcattttttt agctaattgt tcccccgaca
      301 aaatacaagt cacactttcc ttactatcat caaaaaaatt aacatcagaa agcaagacat
      361 catagacacg tcgcatttcc tctagataat tccttattgt cctgaaaagt ttgtctttca
      421 ttgattcaag agtgtcaaaa agtacttttg attcctcttt caattttcga aaatgtgtag
      481 gatacaagtc taggttctct aatagccaat taaatgtttc tacccgactg tctttcttta
      541 aaattagcat cacaactcca ataaaatttt cactgatatc acaattactg gaatgacaat
      601 aaaaaatgga atgcccagtt ttactggtaa aaatactagc agagggatta tgatcgttgt
      661 gaaacagaca tcttaagttt ttacttgact taagtaattt attcatatca atggttaaca
      721 atctttcata aatctcatta aaagacatgt cggaggaatc aaacaggcag tctttaaacc
      781 actgtttcat ttcttctttg tcgtcatttt ttatcaactt aacaaaatcg gtttcattga
      841 ctagttcata ctctctccct gaatcacttt gacaattttt agaacatgtc atggtaaaat
      901 caatcggaag ctcagtaata tctaacatgt tgtcttcatt aattattatc actgcatctg
      961 acttatatcc tccaaaaaac attctattcg aagcatttac attgtcatca cacccgggga
     1021 tgttcttttt caagtattcg taaacagcaa ccattttacc atagtcagta attgcttctt
     1081 ttaatagaaa gacaatcttg tatttttcta gattttcaga gtaccgaata cttttttgta
     1141 tcgcaaacgc agaattatga actaaattga tagataaaac cttttctatg cttaagtatc
     1201 tactatcatc tttactcaaa tctactacct tccccttctc atctttaaca gtgttatcga
     1261 catccagaat aattactctg gtttccacaa aactctcctt gtcattgcct ttattctttg
     1321 tcttgcttgg aataaatgat tgtgatgaat ttagtatctc tacaaactca tctcttgtaa
     1381 cttctctata gttatctaaa cacctatctc ttacatcagt tttttctttt tttgtaactg
     1441 gcttctctaa taatttttct tgatctacat aaattggaat attaatcatt gatgtttcca
     1501 tttgtgtttc ctccttataa aattctgtta aagactaaaa catacagata ataaaaacaa
     1561 actctttatt actatatgtt ttagtcttat ttgtttactc aacctttata ccgttccatg
     1621 aaactatcaa tatcagattt tttaaatcta atgaccgtat caatttttat aacaggtaac
     1681 ccgtattgag aaacatattt tctaatcgtt cctactgata cacctagata ttcacttgcc
     1741 tgctgatagt ccatatactc tagagtgact ccagaaataa tgtcttctag cataggcttt
     1801 agcactgtaa ttatctcttt cacttgttgt tcaacttgta tagaaacaat gctatcaatg
     1861 atttgtagag acatatttcc ccctcctttc gtgattgctt gaacaaattt tctaagaatt
     1921 atcttcgtat tttttgctca aaataaaagt attttgttat taaccgatta atcaaacaaa
     1981 aaatgattac cagttcatcg acatacacat aatacctcga taaaaaaggg gtgtcaacag
     2041 ctttgtaaaa tttatttaca acaccccttt tttgaggtat actaatttta ggaggtgtgg
     2101 tcaatatgat aaaacgtcca ccaataaatt atttagaacg aaaaaagata ctcggaacaa
     2161 aaataaaagc tataagaaag agtaaaaaat taacacaacc agcatttggt cttatgatta
     2221 acaacggtca attaattgac aaaaaaacta tctacgaatg ggagaaggga acctatttac
     2281 caataccaga gcgattatcc cgaattgctg atttgggaaa tatgagtata gaggagttag
     2341 tttgtggcaa tgtagaagaa tatattttgg gaattattct ttatagagat agcattgtac
     2401 tagatggtat aacatttcca gataaaaacc tgttccagca tcttcgtcaa cagttcccac
     2461 cagtacactc taatttagat acatggctgg atagatattc caaattagaa ccagagatgc
     2521 aagaatttat tgctaacaag acttgtaata aagttaaaaa tgaaaaaatt agtcttttca
     2581 atatcttaaa aatagaagaa ttattcatca atgctattgt agaagaattt gacaataaca
     2641 ttcttttcct cactagcagt atagaagaac tactagaaag aatggtagat gaatggctac
     2701 ctattcaatt gaaagacatg tcttatcccg aagaagctgt gagagaaatt actgataata
     2761 taaacaagct agaacaaacg atttcatcaa ttgggaaaaa atacactaag aaaaaaatga
     2821 agggaggtga taccatctag tggtaagtac tccttgtaaa ctaagaggag atgatttaat
     2881 ggcaaaaata tcaaagtaca aaaagaaaaa cggtcaagtg gcgtggatgt ataaaggaca
     2941 cattgccacc gatccacgaa ctggtgaaaa aataaatact acgagaagag gttttagtag
     3001 caaacaagaa gctcaaaaag attatgacga gtacagacat caaatattat acggtgttaa
     3061 aaagaaagca cctgatatga catttgaaga cctgtataat gaatggataa ctcatcagcg
     3121 taccagtgtt aaagcttcaa caatcgcaat ctcagtaaga tacgcaaata atcaaatact
     3181 tccagcattt ggtaagctta aactatcgaa tatctctgtc ccatattgtc aaaaagtggt
     3241 agatgaatgg cacagtaaat atgaaagtta cgattatatg agaaaacaga ctgcacaaat
     3301 tttacgctac ggggttgcaa tgcaatatat tgacaataat cctatggaaa aaacacttct
     3361 tccacgaaaa aaagagtatg agaaaaatcg taaattttac tcaaaagagg agcttaataa
     3421 tttactggat gcttttaaag attttggtaa catgaaacaa tacgccttct tcagattatt
     3481 ggcatacaca ggtatgagaa agagtgaagt actcgcttta cagtggaaag acatagacac
     3541 gtttaataaa gaactacatg tcaataaaac tcttgctgta gatgaatttg gtaaagttat
     3601 aattcaatct ccgaaaacaa gagcttctag acgagttatc tctttagata ctgagactct
     3661 ttctatcctc aacaactgga aattacagca aaaagaagag tatttaaaac ttggatacaa
     3721 tactagctct aaagagcaac atgtttttac aacagttaaa aacactctat acattccaaa
     3781 cactgttaat gattggttaa gatatatttt gaaaaaatat aacttgcctc gtattacacc
     3841 tcatgggttt agacatacac atgctagttt attattagaa gcaggagaat ctgtcaaggt
     3901 agttcaacaa cgattagggc atgaaaatag taaagtgaca ttagacatct atgcacatat
     3961 tacaaataat gcacctaaga aaacaggtca agactttgct gatatgatgg ctcatcagta
     4021 aaagagctgg tcaaaatctg gtcatttcta gtatacaggc aaaacaaaaa agctagaacc
     4081 cttatatatc aagggttcta gctcctataa caggtttacc caacactgcc ttccatctca
     4141 taactgatca atcgattgag ttcgactgca tattccattg gaagttcttt ggtgaatggt
     4201 tcaacaaagc ccatgacgat catttcagtt gcttcagatt cggtcaggcc tcggctcatc
     4261 agatagtaga gttgttcttc tgagattttt gagactttgg cttcgtgttc taaagagact
     4321 tggctgttgt ggatctcatt gaatgggatc gtatcggatt tcgaccattc gtccatgatg
     4381 atcgtgtcac attcgatatg agagatcgaa cccgcactgt ttttggcaaa ggttacttgt
     4441 cctcgatagt ttacttctcc gccgtctttt gaaattgatt tagagacgat agagctagat
     4501 gtatttggtg cattgtggat catttttgca cccgtatctt ggatttggtt tgctcctgca
     4561 aaggcgatgg agagcatggt gcctcgtgca ccttttccat ctaagtaaac acttgggtat
     4621 ttcatcgttg tttttgcacc taggtttccg tcgatccatt caacggttgc gccttcgtat
     4681 gcttttgctc gtttggttac taagttgtac acattgtctg accagttttg gatcgttgtg
     4741 taacggcaat acgcatcttt tcttgtaaag atttctacga tggcggcatg caggctgtta
     4801 cttgaataag taggtgccgt acaaccttcg acatagtgga tgcttgctcc ttcgtcaaca
     4861 atgatcagcg tacgttcgaa ttgccccgtg ttttccgcat tgatccggaa gtacgtttgt
     4921 aatggaacat cgactttgac gccttttggc acgtaaataa aggtcccacc tgaccatacg
     4981 gctgaattca gcgctgctaa tttgttgtcg gttggcggta caagttttga aaagtactct
     5041 ttgaataatt cagggtattc tttcaaagca gagtcagtgt cagtaaagac gattcctaac
     5101 ttttcgaatt catctttcat attatggtac acgacttctg attcatattg tgcggaagca
     5161 ccggctagat aagctcgttc agcttctggt ataccaatct tttcaaatgt atctttgatt
     5221 ttttctggta cgtcttccca gtcacgagcg ggtttgtcac ttggtttttg gtaatatttg
     5281 atggcatcga aatcgatgtc tgaaaggtcg gggccccatg tttgcattgg cattttgtta
     5341 aatgtttcta gagatttcag acggaattca agcatccatt ctggttcgcc tttgattctt
     5401 gacatctcac ggatcacttc ttctgtcaag ccttctcctg tactgaaaac cggttcgaca
     5461 tcatcgtgaa acccgaattt atattcttct aattctggta cgcccatgtt ctcactcctc
     5521 ttccttgtgg taatgatgga aagctgcggt accatcgttt tccattgctt ttcctagtgc
     5581 tttccaggca agtgtcgcac atttgatccg agcgggaaat ttagctacac caccaagcat
     5641 agcagcatcg cctaattggt cgggatcttt gacttctttt ccttggacaa gctgtagaaa
     5701 gtctacagtt aaatgttctg cttcttctat tgattttccg atgacggcgt ctgtcatcat
     5761 cgaggcactt gccgtactga tggagcagcc actgccgtca aaggcgatat ttttgatgat
     5821 tccgtctttt acatcgactt gcaattcgat cacatctcca catgtggggt tgttcaattc
     5881 gatcttgctt gtcgagtcag tcaagcttcc acgatgatgc ggatgggaag aatgatccaa
     5941 gatcacttga cggtataaat tgtctaattt agatagtccc atgttggaaa aactcctttg
     6001 ttgccatgat tgcttcaact aaacggtcgg cgtcttgcgt tgtgttgtag aaataaaagc
     6061 tcgcgcgagc agtcgctgat acttcaaggt acttcaatag cggttgcgca caatgatgac
     6121 ctgctctgac ggctacccct tccatgtcta aagctgtcgc gacatcatgg ggatgtaacc
     6181 catccaaatt gaatgcaacg actcctgtcc gaaaattcgg gtcttgtgga ccataaacag
     6241 tcaatccatc aattgcttgt aattttggca acacataagc ggctagttct tcttcatatc
     6301 gatggatatt gtccataccg agtttttgca aataatcgat tgcatggcct aatgcgatag
     6361 cgccggcgat atttggtgtc cctgcttcga atttccaagg tagttctttc catgtactgt
     6421 cgtataattc gacaaaatcg atcatttcgc caccaaattc aacaggttcc atttcttcta
     6481 accattttcg tttaccatat aaaacaccaa tacctgttgg tccgcacatt ttgtgtccgc
     6541 tgaaagcaaa gaaatcacag tctagtgctt ggacatcaat ggccatgtgc ggggcagatt
     6601 gagcaccatc tactacgatg accccgccgt tttcatggac aagttcagct aattcttgaa
     6661 caggattgaa gacacctaag acattggata catgagcaat ggagacgatt ttggttttgt
     6721 ccgtaatctt tttcgctgca tcctccatat ccaggaagcc ttcttctgtt agttcgatat
     6781 atttcaagac agcacctgtt cgttttgcca actgctgcca cgggataaca ttagaatgat
     6841 gttccatgta agagagcact atctcgtctc ctgctttgat atagcggtcg ccgaaggttc
     6901 tggctaccca attcaagctt gtcgtcgtac cacgagtaaa cagcacttct gctgtttctt
     6961 ttgcatggat gaacgcccgt actttttctc ttgcttgttc ataatcatgg gtcgcgcgtt
     7021 ctgctaacgt atggacaccg cgatgaacat tggcgttatc gtgttcgtaa tagtacgcta
     7081 atgcctggag tacttgtttt ggcttttggg tcgtcgctgc attatccaga taaactaaag
     7141 gttcatcatt gacgtcttga aataagatag gaaaatcatt gcgtagcttt ttagcatcga
     7201 tcatccattc aacttccctt cgattacttc gactaattct ttccgaactt gttctacagg
     7261 gattgctgtt aagacagaac cgaggaatcc tcgaatcact aagcgctctg cttcttcttt
     7321 atgcaatcct ctactcatca gatagtacat ttcttctgga tcaactcgtc ctacactggc
     7381 agcatgtcca gcagttactt cgttttcatc gatcaaaaga ataggattcg catcaccacg
     7441 tgctttatca gaaagcatca agacacgact ttcttgttgg gcatctgcac cttttgcccc
     7501 tttcaagatg tggccgatac cattaaaggt caatgttcct ttttctcgga tcactccgtg
     7561 ttgcaaaata tgcccgatcg tatgaggggc tttattggtt actcgtgtat cgattccttg
     7621 gatttgtctg cccgagctga ttgccacgat tttgacttct gcgtgtgcac cttctcctgc
     7681 taaatctgag tcaaagtcag cgatcacatg accgtcattc atgacaccaa ttgcccagtc
     7741 gacagaagca tctcgtaaaa tgtgcccacg gcgattcata taagaagtaa tattttctcc
     7801 taattgatct acggctgaat atttgatttt tgaaccagct ttagcgatga cttccacgat
     7861 gatattacct gatgattttg ctacttgttc tttggttgtc tggaaacgtt ccagataaga
     7921 aaattcacta tgttcatctg ccacgatcaa cacatgcttg aagaagtgct catcggatgc
     7981 gccatcttga atgaataagg actcgagtgg ttcatcgatc acgacatttt taggcacata
     8041 caggaaaata ccgctgttca taaaagctac atggagtgca gtcaattggt cttcgttcat
     8101 gtttactgct tttttcatat aatattcttg gactagttct ggatattcga tcattgcagt
     8161 gaacaagtcg gtaaaaatga ctcctttttc tgctaattcc acaggcagtt gttcaaaaat
     8221 cgtccatgaa ccttgttgga caagcacagg attgtctttc attgcatcaa atgccggaat
     8281 cgtgcctgtt tcactgatgg tttgtgtttc tttcacatca aataacgacc agcgatggaa
     8341 tttcacccga tcgatatgcg gcaatggcag ttcatctgct ttcgctagtg cgtcttgacg
     8401 gaaggttctc atccaatcag gttcgccttt acttaaagaa aaatcattta ccgcgtcaag
     8461 ataatcgagc cagttttttt ctttcattct tgctccccct tacgcttctt cttctttata
     8521 gtcgattcct aattctttac tgataccagc gtagccttct gcttccagac gtttagctag
     8581 gtcagcattc cctgtcatga ctactcggcc ttccatcatg atgtgaacga catcaggtgt
     8641 gatatagttc aataatcgtt gatagtgggt gatgatcaat gcaccgaaat tgtctccgcg
     8701 catttcattt actcctttag caacgacttt caaagcatcg atatccagac ctgaatcgat
     8761 ttcgtctaaa attgcaaatg taggttctaa catcaacaat tgcaggatct cattccgttt
     8821 cttttctccg ccagaaaaac cttcgttcag ataacgttca gccatttctt ctggcatatt
     8881 tagtaaagcc attttttcat ctaacttttt caaaaatgcc ataacagaaa ttttgtcgtt
     8941 ttcatcacgt ttcgcattga tcgctgcacg catgaattcc gcattcgtga tacctggtat
     9001 ctcacttgga tattgcatag ctaaaaataa gccaagtcgc gcacgttcat ctacttccaa
     9061 gtctaaaaca tttttcccgt caaacaaaat ttcgccttct gttacttcat agtttggatt
     9121 gcccatgata gcggcagata aggtagattt tcctgtgccg ttaggtccca tgatggcatg
     9181 gatttctccg gttttcattg ttaggtttac gcctttaagg atttcttttt cctcgatcga
     9241 cacatgtaga ttttttattt ctaagacaga catgcattat acccccaaat tctctttcgt
     9301 tctcactcat tttagactaa ttgagaatga agcgcaatga ccttccttac taaaatcaaa
//
LOCUS       DAXWLB010000110         9273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXWLB010000110 DAXWLB010000000
VERSION     DAXWLB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.110"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(4..672)
                     /locus_tag="ACGE4H_002021"
     CDS             complement(4..672)
                     /locus_tag="ACGE4H_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8365423.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            806..1093
                     /locus_tag="ACGE4H_002022"
     CDS             806..1093
                     /locus_tag="ACGE4H_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365424.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1509..3047
                     /locus_tag="ACGE4H_002023"
     CDS             1509..3047
                     /locus_tag="ACGE4H_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFF8365425.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3044..4009
                     /locus_tag="ACGE4H_002024"
     CDS             3044..4009
                     /locus_tag="ACGE4H_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365426.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3996..5318
                     /locus_tag="ACGE4H_002025"
     CDS             3996..5318
                     /locus_tag="ACGE4H_002025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8365427.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5381..5485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5559..6302
                     /locus_tag="ACGE4H_002026"
     CDS             5559..6302
                     /locus_tag="ACGE4H_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365428.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6317..7309
                     /locus_tag="ACGE4H_002027"
     CDS             6317..7309
                     /locus_tag="ACGE4H_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8365429.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7321..8058
                     /locus_tag="ACGE4H_002028"
     CDS             7321..8058
                     /locus_tag="ACGE4H_002028"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365430.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8048..8770
                     /locus_tag="ACGE4H_002029"
     CDS             8048..8770
                     /locus_tag="ACGE4H_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8365431.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8854..>9273)
                     /locus_tag="ACGE4H_002030"
     CDS             complement(8854..>9273)
                     /locus_tag="ACGE4H_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFF8365432.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3031 a         1603 c         1962 g         2677 t
ORIGIN      
        1 gaatcagtgc tctaactgtt caagaggatc aagtggttca aatgtttctg tagtctgttt
       61 tgcttccaat tggggcaaaa cagatccaat ttccagatac ttcactttat tcaatgtata
      121 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
      181 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
      241 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
      301 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
      361 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
      421 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
      481 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
      541 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
      601 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
      661 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
      721 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
      781 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
      841 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
      901 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
      961 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
     1021 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
     1081 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     1141 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     1201 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
     1261 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
     1321 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
     1381 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
     1441 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
     1501 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
     1561 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
     1621 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
     1681 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
     1741 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
     1801 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
     1861 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
     1921 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
     1981 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
     2041 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
     2101 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
     2161 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
     2221 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
     2281 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
     2341 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
     2401 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
     2461 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
     2521 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
     2581 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
     2641 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
     2701 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
     2761 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
     2821 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
     2881 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
     2941 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
     3001 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
     3061 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
     3121 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
     3181 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
     3241 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
     3301 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
     3361 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
     3421 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
     3481 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
     3541 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
     3601 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
     3661 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
     3721 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
     3781 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
     3841 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
     3901 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
     3961 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
     4021 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
     4081 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
     4141 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
     4201 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
     4261 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
     4321 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
     4381 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
     4441 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
     4501 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
     4561 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
     4621 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
     4681 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
     4741 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
     4801 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
     4861 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
     4921 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
     4981 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
     5041 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
     5101 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
     5161 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
     5221 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
     5281 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
     5341 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
     5401 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
     5461 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
     5521 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
     5581 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
     5641 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca gatgcacaag
     5701 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
     5761 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
     5821 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
     5881 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
     5941 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
     6001 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
     6061 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
     6121 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
     6181 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
     6241 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
     6301 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
     6361 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
     6421 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
     6481 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
     6541 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
     6601 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
     6661 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
     6721 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
     6781 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
     6841 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
     6901 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
     6961 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
     7021 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
     7081 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
     7141 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
     7201 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
     7261 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
     7321 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
     7381 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
     7441 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
     7501 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
     7561 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
     7621 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
     7681 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
     7741 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
     7801 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
     7861 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
     7921 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
     7981 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
     8041 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
     8101 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
     8161 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
     8221 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
     8281 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
     8341 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
     8401 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
     8461 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
     8521 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
     8581 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
     8641 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
     8701 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
     8761 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
     8821 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
     8881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
     8941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
     9001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
     9061 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
     9121 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
     9181 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
     9241 ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DAXWLB010000111         9001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXWLB010000111 DAXWLB010000000
VERSION     DAXWLB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.111"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(69..1208)
                     /locus_tag="ACGE4H_002031"
     CDS             complement(69..1208)
                     /locus_tag="ACGE4H_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFF8365433.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE4H_002032"
     CDS             complement(1607..2905)
                     /gene="eno"
                     /locus_tag="ACGE4H_002032"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFF8365434.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE4H_002033"
     CDS             complement(3021..3776)
                     /gene="tpiA"
                     /locus_tag="ACGE4H_002033"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFF8365435.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE4H_002034"
     CDS             complement(3880..5070)
                     /gene="pgk"
                     /locus_tag="ACGE4H_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFF8365436.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE4H_002035"
     CDS             complement(5180..6181)
                     /gene="gap"
                     /locus_tag="ACGE4H_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFF8365437.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6222..7313)
                     /locus_tag="ACGE4H_002036"
     CDS             complement(6222..7313)
                     /locus_tag="ACGE4H_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFF8365438.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE4H_002037"
     CDS             complement(7462..8796)
                     /gene="rpoN"
                     /locus_tag="ACGE4H_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFF8365439.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8856..8927
                     /locus_tag="ACGE4H_002038"
     tRNA            8856..8927
                     /locus_tag="ACGE4H_002038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8889..8891,aa:Arg,seq:ccg)
BASE COUNT         2336 a         1905 c         1658 g         3102 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtttatta
       61 aaaaaatctt acgctaagta ggataaaata attccttgat atttttcgat catatccaaa
      121 taattatcga tttcaacata ctcatctact tgatgaggca aagtaacgac accaggccca
      181 aagacgacaa agtcgaagga atggttagct ttcgtaaatt cggctgcatc cgttgttgct
      241 gccgctccaa ctaaaggtaa tggctggctg aattgttgct gaatgcaatg aatcaaagga
      301 gaatctggat cagctttgac aggtattttg ttgtaatcga tcatcaattc caaatgatag
      361 tctgtctctt gattcaattc attgacgatt gattgcagca atgcaatgat tttatcattt
      421 gggtattctg gaatcgatcg aatgttccct tggagctgcg catggcttgg gatgctgttg
      481 acttgattcc cgccactgat caatgtaaca ttatggattg tttttcctag gacaggattc
      541 tcgattgttt cagcgaggtg attcatttca gcatttgctt tcgtaatgaa ttcatttagg
      601 tgattgattg cattataacc ttggtctggc atagagctat gcgcttcttt tccgtgcgag
      661 gtgactgtat agttgattga tcccatgtgc gtatacatca aactgtaatt ggttggctct
      721 ccgatgatca aggcatctaa gtcatctaca tagcctgctt tcgtgagctg ttccccacct
      781 agttcaccca cttcttctcc gacagtggct aataatttga ctgttccgtt aaatggtttt
      841 cctgactctt tcagttcgat catggcaatc accattgctg ccaagccact cttcatatct
      901 gtagcaccac gtccgtacaa acggttccca tggatttctg ctgcaaaggg tgcataggtc
      961 caagacgatt catcaccagc cgccacgaca tccatatgcc cagataggcc taacactttt
     1021 cctgattggc ccttttggta acgagcaatc aaattatctc taccatctct gtaagaaaca
     1081 atctctcctg ttatgtcatg ctttgccaaa agcttattca aataggctgc tacttcacct
     1141 tcgtttccat tcactgattt aattcggata atctcttgta aaatcgcgat tttttcttct
     1201 tttttcatct aatcctctcc cttgccattt ctttttattg tatttgagtg tgctcgaatt
     1261 ttcaaatagg agcaaccaaa gtaaaaatga agaaagagag ccactttatg aaaaatatgc
     1321 tgctacttcc ctcctatttt atttatacga gaggacttgc ccatagattc ctattctact
     1381 gttctatcct taaaagctct tatatgatgc tagttattgt tagctttcct ttttcaagga
     1441 tgatcttttc ttgtaccatt ctttgtaaac atcatataca gaagctagtt gcaaaaaaac
     1501 tctctacctg ctgatatttc agcaagtaga gagttcgatt acttaactaa gtgatcatac
     1561 tcgactatct ttcaatagtc gatcatgtat cttcattaga aaagaattat ttgtttttta
     1621 agttgtagaa agatttcaaa cctttgtatt ctgcaacttc accaagttgg tcttcgatac
     1681 gtagtaattg gttgtattta gcaatacggt cagtacgtga tagagaacca gttttgattt
     1741 ggccagcgtt tgttgcaaca gcgatatcag agattgttga atcttctgtt tcaccagaac
     1801 ggtgagatac aactgctgtg tagccagctt ctttagccat ttcgatagct tcgaaagttt
     1861 ctgttaatgt accgatttgg ttcactttga taaggattga gttagcaatt cctttttcga
     1921 taccttcaga taatttttga gtgtttgtta cgaacaagtc gtcaccaact aattgaactt
     1981 tgtcgcctaa tacgtcagtt aatttcttga atccgtccca gtcgttttcg tctaatccat
     2041 cttcgattga gatgattgga tatttagaaa ctaattcttc gtagaatttg atcatttcgt
     2101 cagttgtttt ttcgccttcg cctgagtcag ctagtacgta aacacctttt tctttgtcgt
     2161 agaattctga agaagcagca tccatagcaa gaacaacgtc tttaccaggt acatagccag
     2221 ctttttcgat tgcttcgatg attacttcaa aaccttcttc gtttgaacca aggtttggag
     2281 caaatccacc ttcgtcacca acagaagttg ctaatccgcg agctttcaag attgaagcta
     2341 atgcgtggaa tacttcagca cccatacgta gagcttcttt gaatgttgga gcgccaacag
     2401 gcatgatcat gaattcttgg aagtcgattg agttatcagc atgtgatccg ccgttgatga
     2461 tgttcatcat tggtgttggc aatacttttg tgttgaatcc gcctaggtag tggtataaag
     2521 gtacttctag gtagtcagca gctgcacgtg caacagcgat agaaacacct aaaatagcgt
     2581 tcgcacctaa tttacctttg ttaggagttc cgtctaaagc gatcatagct ttgtcgatag
     2641 ccatttggtc acgaacatcg tagccaatga ttgcttcagc aatgatgttg tttacgttgt
     2701 cgacagcttt agttacacct ttaccgccat aacgagcttt gtctccgtca cgtaattcaa
     2761 ctgcttcgta ttcaccagtt gaagcacctg aaggaaccat tccgcggcca aaagctccgc
     2821 tttctgtgta tacttctact tcgattgttg ggttaccacg tgagtctaag atctcgcgag
     2881 cgtaaacatc agtaataatt gacatgtttt gtctctcctt tgagtttgtt ttttcctaag
     2941 ggatttccct catatacgat tttagttaat tttgctcatt agtgcaaaca tttcttgcat
     3001 ttttcgcaaa ttttcaacaa ttattttaca gcgtctaaca atgacaagaa tgaatctgct
     3061 tgcaagcttg cgccaccgac taaagcaccg tcaacatttt cttttgccat gtattcagca
     3121 atattttctg gttttacaga accgccgtat tggatacgaa ctgcttctga tacttctttt
     3181 ccataaagtt tttcaactgt ttgacgtaca actccacaaa tttcgtcagc gatttgtgcg
     3241 tcagctgatt taccagtacc gattgcccag attggttcat aagcaataac catgtttgac
     3301 acctgttcag ctgaaagatc agctaaacca gctgtaattt gtccttcgat ccattcagca
     3361 gttttgcctg cttcataggt ttctaaagat tcgccgcagc ataggattgg tgtcatacca
     3421 tttgcaaaga tagctttcgc ttttttattg atgtcttcgt ctgtttcatg gaaatattca
     3481 cgacgttctg aatgtccgat gatcacataa tgaacaccta gatcagcaag agctgctggt
     3541 gaagtttcac cagtgaacgc acctgagttt tcccagtagc agttttgtgc tgagattttt
     3601 agttctgtac cttcagctga tgtcattaat tcttgtaaga ataatgcagg agatccgatg
     3661 actgaatcaa ctttgtcatt tgaagggatt ttgtttttta ctgcttcagc aaattctttt
     3721 gcttctgatg cagttttgtt cattttccag ttacctgcga taataggttt acgcattgag
     3781 gaacacactc cttaatttat tgaaaaatgg ctagggagtc atgaaaactc ccttcacctt
     3841 ttgttcgatt actcgtcaga attgagcaaa cttggtcgtt tatttatcgt tgattgctgc
     3901 tagtcctggt aattctttac cttcaagcaa ttccaagctt gcgccgccac ctgttgagat
     3961 gtgtgtgaat ttgtcagcaa atcctaattg ttctgctgct gcggctgagt ctccgccacc
     4021 gatgattgtt gttgcgcctt ctaagttagc gattgcttca caaacaccga ttgtaccttt
     4081 agcaaagtta ctcatttcaa atacacccat aggtccgttc caaacaactg tttttgcacc
     4141 ttgtaatgta tcagcaaata attttactga tgctgggccg atgtctaaag ccatcaaacc
     4201 atcaggaatg tctccttcga ttacttgtgt ttcaacatca tttgaaaatt ctggtgcaca
     4261 gacattgtcg attggcaaga caagtttgtc gcctgctttt tcgattaatt cttttgctag
     4321 ttccacttta tcagcttcta ctagagagtt accgatcttg attccttttg cttcgtagaa
     4381 tgtgtaagtc attccgccac cgatcaagat tttatctgct ttaggaatca agttttcgat
     4441 tacgccgatt ttatcagaaa cttttgcgcc accaaggatt gcaatcattg gtcgttttgg
     4501 ttcttctact gcttcaccga tgaacttgat ttctttttcc atcaagaatc cagcaactgt
     4561 tgggatacca gttgaagcga tacctacgtt agaagcatgt gcacggtgag ctgtaccgaa
     4621 agcatcgtta acaaatacat cgcctaatga agcccagtat ttacctagtt ctgtatcatt
     4681 tttgctttct tttttaccgt caatatcttc aaatcgtgta ttttcgaata cgacaacgtc
     4741 gccgtcattc atgttgcgga ttgcttcttc taattgttct ccgcgagttt caggtacgaa
     4801 tgttacttct ttacctaata attcgcctag acgttttgca actggagcta aagattttcc
     4861 ttctttatct tcttctgttt ttacacgtcc aaggtgagag aaaaggattg cttttccgcc
     4921 ttgttctaga acgtagttga ttgttggcaa tgctgctttg atacgagtgt cgtcagtgat
     4981 cacgccgtct ttcaaaggaa cgttgaagtc aacacggaca agaacttttt tgtcttttaa
     5041 atcgatatct ttcactgttt tcttagccat tttatttcct cctgaataaa cttattatca
     5101 aaaaaagcgg aagaagcgcg attgcttccc cgcttttaaa tgtacatgtc tataatttac
     5161 acttgtacgt ttgtctatct tataagttag cgaagtattc taaagtacgt actaattgtg
     5221 cagtgtatga catttcgttg tcgtaccaag caacagtttt aactaattgt ttgtcgccaa
     5281 ctgtcattac tttagtttgt gtagcatcaa ataatgaacc gtaagtcata ccaacgatat
     5341 ctgaagaaac gatttcgtct gtgttgtatc cgtaagattc gtttgaagct tctttcattg
     5401 ctgcattgat ttcgtcaaca gttacttctt tttcaagaac tgttactaat tctgttaatg
     5461 aacctgttgg tacaggaaca cgttgagcag ctccgtctaa tttaccgttc aattcgggaa
     5521 ttactaaacc gatagcttta gcagcacctg ttgagttagg aacgatgttt gcagcagcag
     5581 cgcgtgcacg gcggaagtca cctttaggat gaggtccgtc tagagtcatt tggtcacctg
     5641 tgtaagcgtg gatagttgtc attaatcctt caacaacacc aaatttgtcg ttcaatgttt
     5701 tagccatagg agctaaacag ttagtagtac atgaagcacc tgagataact gtttctttac
     5761 cagttaatgt ttcgtggttt gtgttataaa cgattgttgg aacatcgttt ccgccaggag
     5821 cagagataac aacgcgttta gcaccagctg ttaaatgttt ttcagctgct gatttagaag
     5881 tgaagaaacc agtacattct agaacgatat ctacgcctag ttcgccccat ggtaattctt
     5941 ctgggttgcg gttagctaat actttaactt cttttccgtt tacgttgaat gaaccttcat
     6001 gaacttcaac tgttccgttg aaacgtcctt gagttgtgtc gtattttaac aagtgtgcca
     6061 acattttagc atctgtcaag tcgttgattg ctactacttc gattccatct acatcttgga
     6121 tacgacggaa tgctagacgt ccaatacgtc caaaaccatt aatacctact ttaactgtca
     6181 tttaagattt cctccttatg aaaatcaaaa aaatatttat tttaaagggt tacccctttt
     6241 aaaatctcat ttgcagcagc ttcatcagtg atgagccacg tttgttttgg tgcatttttc
     6301 atataagctc gaattgcttt agcttttgat tttccgcccg cgattgccat gatgatcggg
     6361 actttttcca aatcttgcaa ttctatgccg attctaggga ccttataaac gacttcacct
     6421 ttttcattga aaaagtagcc aaaagattcc gcaacagcat ttgcttgttt cagcatgact
     6481 aattcttttt cagtcatctt acgacgtgct gccatgtgta atgcacgccc aatactatga
     6541 ataacacaat tcgcttcact aatcaagttt aagacttctt ggatagatgg ctcattgagt
     6601 aaagaattgt aagttgccag acttaattgt tccggaacat aaagtgcgcg gtggttaccg
     6661 tttgctttag cagccattct tgcactgaca gaatttgcct gtacagttat cgcttcacca
     6721 atcccgcctc ttgcagggac aaacaggtta tgccgtttct tattttccag attagacaat
     6781 tgttcggcta ccacagccat agtagttcct cccatcacag cgatgatatt ctcgccttct
     6841 ggaagtagtc ggtctaaggc ttcgctcagc gcttcgccga atgcatcagc cactttggtc
     6901 tgctcttcgc tgtttcctga aacaacgata cagcgttggt taccaaaata ttctgccaac
     6961 tgttgttcgg tttgatgcgt acctaaaagc tgatccatga agttttcaag gctttgataa
     7021 acttcttcgc ccttagttgt cagcgtcata ccgctttttg aggtgtcgat caaattcaga
     7081 tttttcaaaa ggtctgtttc tgttcgtaag actcgttctg tgatccccat actttccgat
     7141 aaacttcgac gcccaatcgg ctgcatccaa tagatatttc gtaaaatttt atagcgttct
     7201 tgcaacactt cgataatatc tggtgcaaca gattcaatca tttttatttc atccagcata
     7261 gcgatctcct tagttggaca ctttacgtcc aatgtagact taaaacgacc catatacgat
     7321 aaaaaaagaa attaactatt cgaatttcac atcaataaag ggaaatctca acctcacgac
     7381 tgtaagtcta acagttttgc tctcgttttt caactaaaaa gactagaaaa agtgaaatac
     7441 tttttctagt cttttgactg attagttatc ataacgtttt ctttttgatg aactcgggat
     7501 tcctaataaa tcacgatatt ttgctactgt tcgtcttgag atcgctatct catctttttt
     7561 cagcaattca accaatttct ggtcagacaa cggcttgttt ttgtcttctt gatcgactaa
     7621 ttcctgcagc tttttcttcg ctgtatctgc tgaaaggtct tctgtctgtt ctgtactatt
     7681 cgtaggaatt cgtgtagtga aaaatttttt taattcaaat acaccgaagt ctgtttccaa
     7741 atatttcccg ttgactgcac gactgaccgt ggattcatgc acatctatct cagttgcaac
     7801 ttcctttaac gtaagaggct tgattggacg ttccttgtcg ataaagaaag ctttttgccg
     7861 ggaaacaatg acttgagcaa cccgcaaaat tgtatcccca cgttgtagga tcgttttctt
     7921 caaccattcg aattcctgtt tccgttcttt taaataattt tcagtttctt tatctgcttg
     7981 ttgtgacatt tgtttgaagt aactctcttg aaagcgtata tttggctgat tttttcggtt
     8041 tgagataact tgtacatttt tatcgtcgtc cactaatacc cggacatcag gaataatgta
     8101 caattcgctg ctacggtcga aaatccggcc agggcttgga ctcaacgttt gtaaatagtc
     8161 gaatatctgc tgaacagcat gtagatcaac agaaaaacgt tgtgcgattt ctttccattt
     8221 tcgctccacc agttcctcaa aacattcttc caacacgata taagctaatt ccggcgcata
     8281 atcatctcgt tctgtttgaa gcatgagaca ttcttgcaaa ctacgtgcac ctactccagc
     8341 tggttctaat tgctgaatca gagtcaacgc atcgagcatt tggataggcg tagctccaat
     8401 ctcttttccg gcttcttcta aactaatcgt caaaaatccg ttcaaatcga tgtattctac
     8461 aagataaaga acgattttcc tcaaaaaagt atctcggtaa ttcaaatgga cttgttcgat
     8521 caatgaatca aataaagacc cttttgtatc aggaatctga tttaggtaat ttgtctcttc
     8581 tccttcataa gaagatgaag aagcttttat gtaatctggc tgccattccg gctcaacaac
     8641 ttccaccaat ggattttcca ttgcttgatt ctcaacgaat gccaacagct cctcactgtt
     8701 atattgtaaa acttggatgg actgttgcaa ctgttgcgtc atggctagtt tctgtgtctg
     8761 ctgctgtttt tgtgaaaaat tttgctcaaa tttcattttt ttgtccaatc cccttgtaat
     8821 tttttatctg tttagttaca attatcccag tgcttgcgtc cttagtgtag tggatatcac
     8881 gtaagattcc ggttcttgag acgggggttc gattccctca ggacgcgttc tactgctatt
     8941 ttagcataat ttacgaatag ttgcttcttt atttttaagt gaaaactgtc cgccaaagaa
     9001 a
//
LOCUS       DAXWLB010000112         8925 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXWLB010000112 DAXWLB010000000
VERSION     DAXWLB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.112"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(33..896)
                     /locus_tag="ACGE4H_002039"
     CDS             complement(33..896)
                     /locus_tag="ACGE4H_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFF8365440.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1092..3191)
                     /locus_tag="ACGE4H_002040"
     CDS             complement(1092..3191)
                     /locus_tag="ACGE4H_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFF8365441.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3411..4520)
                     /gene="rpoD"
                     /locus_tag="ACGE4H_002041"
     CDS             complement(3411..4520)
                     /gene="rpoD"
                     /locus_tag="ACGE4H_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFF8365442.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4544..6385)
                     /gene="dnaG"
                     /locus_tag="ACGE4H_002042"
     CDS             complement(4544..6385)
                     /gene="dnaG"
                     /locus_tag="ACGE4H_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFF8365443.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6567..8882)
                     /locus_tag="ACGE4H_002043"
     CDS             complement(6567..8882)
                     /locus_tag="ACGE4H_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFF8365444.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
BASE COUNT         2277 a         1921 c         1549 g         3178 t
ORIGIN      
        1 taagctagtt aagaaaaaca tgcaagggat tctcaattag attttttgac tagttttgtc
       61 acaccgtttt cttgtgtaat caatccttgt ttcaaaagat tacctaatgc gcgcttaaat
      121 gctcctttgc tgataccaaa agctttcatg atttcttctg gatccgattt atctgtaaag
      181 ttgatttgct gatctgcaga acgttctaaa atggttaaaa tcatttgtgc atcatctgat
      241 atagcttcat atgctcttgg cttcaatgaa agattcagta caccgtctgg tcggacacca
      301 atcactcgac cttttatctg ctcgcctaga cgaggttcat tatatcgttc agaaggatgg
      361 ataaacccaa gatagaaatc ttccgttaaa acgtacgtac ctgttaattt caagcggtat
      421 gctgttccta tcacatcttt gtttttcatt tcttctgttc catatttgct taaagatttg
      481 aatatttttt catctgctaa actgccccaa atacggtctt ttgcatcaac tctaagggaa
      541 atcatcaagc gatccccttt tttaggccat agctctgtca ttgtcggcaa ttcatcaaga
      601 gatacgacaa agtctttatc tggaagaccg atatcgacaa acgcacctaa atcttttcgt
      661 gtagcagtaa cgattccaaa agcgtaatga ccgatacggc ttttaggaat ttgtgtagtg
      721 atccgatttt catgtttttg attttggtaa ccgaatcctt caactgcttc accgattttg
      781 tgttcacctt ctgatttgct taagccaaag gtttgtccgt ttttttggac aaagtagttt
      841 ttttcatttt catcaataat aatacctgta aatatagttg ctaataaatc gttcattttt
      901 ttctccctct atagtgaaag gttttcttaa tacatttctt tgctatcata acataatgtt
      961 atctacttga atagctgagc cttggtttgg aaaggtgaaa aaaggagtat gacaacagtt
     1021 gtgtcatact cccctttttc gaataaacgg tgttaaacgg caggctcaca gcctctttca
     1081 tttgcctaaa attaattgct cccatttcct ccgaaccatc cagttttaca gttgatggtt
     1141 acgatgtaaa atggcaaagc actttctcca atgcctttca tcaatgcaga ttttgttgaa
     1201 gtggcatata gattataata gccttcccca ttttcgattc tggctttttc taatctgtaa
     1261 cctccttcaa cgatatatcc acgttggata catgtagcaa tcactttgct ttggacacct
     1321 ggtgcccacg tccatgctgg attagatgca tcggcaatat cacttgctct ggcagatttg
     1381 attggctgat tcgctgagtt tgaagaggaa ggggtcgagg atgccgcttc ttgctgtgct
     1441 gcttgtgcga ccgcagcttg ttgagcggct tgagctgcag ctgctgcttg agcggcttgt
     1501 tttgcttgtt cttcagctgc ttttttcgct tcttctgctt ttttcttttc ctcttcttct
     1561 tttttctctt tatcagtgag catcttatct acttgtttca attgttcatt caactgagca
     1621 gatagatctg tatttttcac tttttctact gcttcttttg ccgtcttgta ttggtcgcgt
     1681 gttgctgaat ccactacttt tccgtcttta aagactatag ctgttttttc tttagctgtt
     1741 tgcaattcag catattgatt tttagcttct gtttgtgctt gttgaatcaa ttttccaaaa
     1801 tctgtgttat ccgtttcgat tgcttggatc tctttatctg ctttcaactt gatgtcatct
     1861 gctagatgat catcgacgat ggcaggtgct atgaaccact cattcacttt ttggatatct
     1921 cggatcttag attggatatc ttggtaagtt gtttctaact ctgaatatcc tttttcattc
     1981 ttaagactgg ataaatcatt ttttagtttt gataaatctt gatgaatcat atctgcacga
     2041 ataaattgat ggtgatcatc tgtataaaag gcagcaagtt tctcatgaag ttcttccatt
     2101 tgttgctgtc tttttgcttc tgctgctact tcggcttcta ctttttgatg atggtcataa
     2161 taagcccagc ttcctccacc gagtagaagt actgctgcta aaacgagata aggtactttt
     2221 tttgattttt tcttttgcgc tttatctgga tctgtttttt ctaagtttga ttcttgatgt
     2281 gttttttcag tatctggcac gattgttatt gatttcgctt ctgcttcaaa cagcttaggc
     2341 tggttctctt ctttttctat ttttgcttca agatcagtac cctctttgct tacagattga
     2401 ttttcagcat cggattcact atttatttca tcgggctctg ttgacttttt gtctgtgttt
     2461 tcgagtattt cttttttctc tgcttccggc agtttattcg ttttttcctt ttctaattgt
     2521 ttttcagtaa atacttcagt tttttctgtg atatccgttt ttacgtcttt tatttttact
     2581 gtttctttac ctgtggcgtt ttctctatca gagtcttttt cagtttcttg ttccttttct
     2641 ggctctactt tgttttcctg tgcaattttt tcactgatca ttttgagcag ctctttcgcg
     2701 tgctcttctt ttgtatcttc acgatgtgct ttgatataag cggatagtat cgaattttct
     2761 tcctcattat cgacgatttc tttcagttca gctactcttc ttttttgttt tgattcaagc
     2821 gtttcttctt gattctttcc tgattcttcc gaagtctcat gaacagttgc tttcttctgt
     2881 tcatttttct ctatattagt tacttgatcg tgagcaggtg gttcttcgtc ttctggttct
     2941 gctttttcga ataattccat tacagattct agaggtaaat ctttcaattc cgaccattcg
     3001 attggatcgt ttaattctgt atcaggaaag actgcatctt cagttgattt atcctgcata
     3061 gctgcatctg tatttgtttc ttcaattgat tcttttattt gctgatcttc tgcagttatt
     3121 tgatgaccgc agtctggaca gatagtattc tgctccattt ttttcgatcc gcaatttgga
     3181 cattttttca tcacttagcg atcggctcct ttactaggtt tacgctacca tttaatctat
     3241 catattctat acgtcacgcc aatagcttat ttttaggatt tacttaaaaa taatgaatga
     3301 atttccttcc tatagaaaaa aaagcttgag actgcgtagt ctcaagcttt ttttctttta
     3361 aaataaacca aacagtttta ctgatttctc ttctggtcaa tagtccacgc ttattctaag
     3421 aaatctttta attgttttga acgagaagga tggcgcaatt ttcttaatgc tttagcctcg
     3481 atctgacgaa tacgttcccg agtaacacca aatactttcc ctacttcttc caatgttctc
     3541 gtccggccat catctaaacc aaaacgtaag cgaaggacat tctcttcccg atcagtcaat
     3601 gtatctagta catcttccaa ttgttctttt agtaactcat aagctgcgtg ttctgctgga
     3661 cttgttgctt cctggtcttc tatgaaatca cctaagtgag agtcatcttc ttcaccgatt
     3721 ggcgtttcaa gggaaacagg ttcttgcgcg attttcaaga tttctcggac tttttctgtt
     3781 ggaagatcca tttccgcacc gatttcttct ggcgtaggtt ctcttccaag gtcttgcagc
     3841 aattgacgtt ggatacgtat caatttatta attgtttcaa ccatatgaac tggaatacgg
     3901 atcgtacgtg cttggtcagc gattgctcga gtgatggctt gacgaatcca ccatgtagca
     3961 taggttgaaa acttaaatcc tttgcggtag tcaaattttt caactgcctt catcagtccc
     4021 atgtttcctt cttggatcaa gtcaaggaac tgcatgcctc gtcctacata acgtttagcg
     4081 atacttacta ctaaacgaag gtttgcttct gccagacgct gtttcgcttc ttggtcgcct
     4141 tcttcgattt tcaatgctaa cgctacttct tcttccgcag ttaaaaggga gacacggcca
     4201 atttctttca gatacatacg aaccgggtca ttgattttta caccggttgg cgcagaaaga
     4261 tcttctgttt ttgcttgttc agcttttttc tcagcactct tcaagctgtg aattgatgga
     4321 tcaccatttt catccaccac actgatacct gcgtcttcaa tcttttggat caatttgtcc
     4381 atttcatctg cattcaaagt gaatggtgta gcaagctgat tggataaatc atcatataca
     4441 acttgtcgtt tgggtttgtt ttctttgatg aaagctgcta ctgctgcttc atacgttttt
     4501 ttatctgttc catttgccat aaaagaaggc cccccttgag tagctatttg gcttgtttta
     4561 actgtttcgt taaattgatg atttcaatcg tcaattcaag ctcaagctgt ttgtttccag
     4621 ttcgtctggc ttcttgctgc atttgttttt ttaatgttat ttcatcagct aagccagatt
     4681 tgctgatcac atctagcaag tcttggattt ctcgtggcga actttcttct gccaccggta
     4741 aatattcgat ttccaccacc aaccgcctta aattttcatc ttgcaaaaag tctaaaaact
     4801 ctgcaaggtt gaattcataa tgttgtgaga catatgcatc gaataaaaaa taaacttctg
     4861 catatgcatc atgaataaac tgaaattctc gttcttttaa aatattacgg acagaaactt
     4921 ctttaaatgc acgatataat agaatctgct ctgccttttc tacttgcgtc aaaggtctct
     4981 ttttccgtgc agtttcttgt ctcgtaacct gagtgtgttg actttcctga ggttcttgac
     5041 gattttgctg atttgtcctt cgaagatcgc gcagttgttt ttgtatcgtc tctctgccga
     5101 tctgaaactc tgttgacagt tgagatagat acatatcttg ttcgattggt gaaacaactc
     5161 ggctaagttc aactagtaat tcttcgaggt attcgatctt gtctttttca ttatctaaat
     5221 ttttcccttg ttttaaataa cgtgatttaa aagtaaagac tgtttcgcgc ccatgagcca
     5281 ctaattcttg gaaagatgct tctccatact tacggagata ttcatcagga tcaagctttt
     5341 ctggaacgac tacgacagaa agtgctaatc gactgttttg ccgaagcaat tcaatccctc
     5401 gattggttgc ttcaaaacct gcgttgtctc cgtcatagca aaagaccaat tctttcgtca
     5461 gcttttccat ttgttggatc tgttgagctg ttagacttgt ccccatagaa gctaatccag
     5521 ttttgatacc agcctgccat gcagcaatca catccataaa cccttcgaac aacaaaattt
     5581 ctccgttttt ccggatatcg cttcttgctt tatcaagatt aaataaaact tgtcttttat
     5641 tgaacagttc tgtttctgga ctattcaagt atttgggctg atctttttcc tgcccttttt
     5701 caggtgcaag ccagcgtcct gaaaaaccga tcgtctggcc ttgagaatta cgaagtggaa
     5761 acatcactct ttgataaaaa cgatcagaca tacggccatc atcatgcgtg acaaacaatc
     5821 cagagcgttc taacaattca tttgaaattg attcattctt gaatacttgc tgaagaaaag
     5881 aacgctcatt aggtgcaaaa cctatgttga attcttcaat taattctaaa gacaacccac
     5941 gactttgcaa atattctaaa gctggctgtc cagcttttgt atgcaatagc atgtgatgat
     6001 agatttccgc tgctttttca tgagcagcaa tcagtttccc cgtagcagat tcaggatttt
     6061 ggacagctat agtacgctga cgccattgat cttctaacgg tatctgttcg aagtctgcca
     6121 cttttaccac tgcttctgga aaacttagac cttctagttc ttgaagaaaa gtaaacacgt
     6181 ttccgccttt accacaacca aagcaatgaa aaatctgctt gtcttctgca acagaaaaag
     6241 acggtgtacg ttcttcatga aaaggacaca gccccaagta atttttacca gactttttca
     6301 actggacata ttgtccaatc acctcaacga tattcgtttg actgcgtact gtttcgataa
     6361 cctcttgagg aatccgttga gccatttttt caccccctgc tctattgata tcttttgggt
     6421 ttaacagaag atatttcagg agaataaccg gtaaaaaatc gcactaactg aaatcagtgc
     6481 gattttaaag gacacaatta tacatgtttg attgtatcat atttcattgc tttttcaagt
     6541 ataacacatt tctatctcat taagcatcaa tttgctactc ttttcagtaa ctgattttga
     6601 atatcagcct tccagtacaa tactcgaaga acaatattta cgagcatgaa tacaaagaag
     6661 tatcctacgc tctctcccaa ccctaagttt acttccaaaa ttccatggaa cagcattgca
     6721 gctacatata tccctacgat cgtcgttact gctaggaaga tgcgcaatgg gttgaaggga
     6781 taacaagctt ttacaactgc catgcaactg atcccaatca gcaagtaata catcaaagta
     6841 gtagtttcag catttcccca tgcgaatgtt tttccaagta gatacaccgc aatgatattc
     6901 actactacaa gcaaggcatt tggtaaagca ttaataagtg ctgtaggcaa ataacggtaa
     6961 gtgatttttc ttttatcttg ttcaaaagat aaaaagaagg aaggataccc ttcaatcgct
     7021 aaatcaatca gcgtgatttg gatagggata aacggaaatc caattgctgt aacggcacat
     7081 ataatcgata aaataaatga ataaatcgtt ttgataaaaa agatgcctga gactttcgtc
     7141 acattgttga ccactcggcg tccttcaaac aggacttctg gcaatgtagt aaaatcagag
     7201 tccaacaata ccagattcgc aatctgacgt gttgctccat caccttctgc catagcaatg
     7261 ctgcaatctg cttctcttaa cgccaaaaca tcattcacac cgtcgcctgt cattgcaacc
     7321 gttctgccgc tttctttcaa ttcatttacc agcaatttct tttgctgcgg actcactcgt
     7381 ccaaaaaccg tatagcgatg agcagcttct cgcacttcca tttcttcagt aatttgagaa
     7441 agatcgacat acgcatcata attcggtaat cctgcacgtc tagcgatatt ggagacagta
     7501 aaaggattgt ctccagaaat cactttcaga tccacacctt cctcatgaag ataagctagt
     7561 gtttcttgag cgttttttcg gattggatca tcgatttcaa acagagcaac cggctcaaca
     7621 aatgctggtt tttcttcgtt caattgctga tttggagcga tccccaacat aagtacgcga
     7681 tagccattcg cttgggctgt ttttagactg tccggaagcc gatcttcttc caccaaacgt
     7741 tcaggcgctc ctacgtacac gactcccagt tctggaaatt ccatcgcgcc ccatttgcga
     7801 tcagaagaaa atgcgacttt gtttgtcaca tcataccgat tagaaagctc aaaataatct
     7861 ctcatagccc gcatagtgat gttgttatca ctgctttctg ccagatacgt ccccataatt
     7921 tctgggatgg ctttctcgta cagcggatgt agtgcttcta cccgctggat actcattttc
     7981 ccttcagtaa tggtccctgt tttatccaag cataatgtat cgacatgggc cagcgtttca
     8041 attgaataca tgtcttgaac aaggattcgt ttttttgcca gtttggttac ccctgtcgtt
     8101 aatgcaatac ttattagcaa taccaaaccc tttggaagca tgcctaacaa agctgctgca
     8161 gatgcaataa cagaaatttt gatagaagca tcgcggatga acagtgcttc caaaaatagt
     8221 atgatcccca aaggaataat cacgaaacta gtgaatttgg atactttgcg aatcgaatct
     8281 acaagttcag aacgaatagc tttgtgagtc tttgcctcgc tagtcaagcg gacagcataa
     8341 ttatctgcac ctactcggat aacttcagcc atgacagaac cgcttgtcaa aaagcttcca
     8401 gaaagcaatt cagctccagt ttctttgaca atcaaatcag attcgcctgt caaaagagct
     8461 tcatttgctt ctgcccgtcc agatataacc tgtaaatcgg ctggtatctg ttctcctgct
     8521 ccgattttta ccaaatctcc taataccagc tctttagtag aaatcgattg ttctttaccg
     8581 tcccggatca ctagtacttc tgattgggaa acaatcgata atttccgtac aagatttctt
     8641 gcatgtattt cttgataaat tccaataacg atattgacga caataattgc taaatacgcc
     8701 atgttggaat acgcacctac aaataacaga caaatcccaa taccaaagtt cagaaaattg
     8761 aataacgtca atatattatc gctgaatatt tgttttgtac tttttgatgc atcttcctgg
     8821 tagtcatttt gctggccaga agccattttc tgaactacct cttgctgtgt aagtccattc
     8881 attttctttt tctccatttt ccgccaactt tcaaaaataa tcacg
//
LOCUS       DAXWLB010000113         8867 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXWLB010000113 DAXWLB010000000
VERSION     DAXWLB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.113"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..606
                     /locus_tag="ACGE4H_002044"
     CDS             <1..606
                     /locus_tag="ACGE4H_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365445.1"
                     /translation="KEQEEKQRQKEKEREQLEIRKREQDLAEQKMAIEKQRLEQEKEK
                     EAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCI
                     ASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKEYEQQLEELLNRTNQ
                     STTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNEVK"
     gene            606..3218
                     /locus_tag="ACGE4H_002045"
     CDS             606..3218
                     /locus_tag="ACGE4H_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFF8365446.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            3215..5593
                     /locus_tag="ACGE4H_002046"
     CDS             3215..5593
                     /locus_tag="ACGE4H_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFF8365447.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            5654..6196
                     /locus_tag="ACGE4H_002047"
     CDS             5654..6196
                     /locus_tag="ACGE4H_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365448.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            6274..6606
                     /locus_tag="ACGE4H_002048"
     CDS             6274..6606
                     /locus_tag="ACGE4H_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365449.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6607..7200
                     /locus_tag="ACGE4H_002049"
     CDS             6607..7200
                     /locus_tag="ACGE4H_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365450.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7222..>8867
                     /locus_tag="ACGE4H_002050"
     CDS             7222..>8867
                     /locus_tag="ACGE4H_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFF8365451.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECH"
BASE COUNT         3215 a         1418 c         1793 g         2441 t
ORIGIN      
        1 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
       61 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
      121 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
      181 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
      241 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
      301 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
      361 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
      421 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
      481 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
      541 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
      601 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
      661 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
      721 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
      781 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
      841 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
      901 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
      961 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
     1021 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
     1081 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
     1141 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
     1201 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
     1261 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
     1321 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
     1381 tgagtgataa tccattggca ctcattgtga aatactggaa gcgaggagac atagacaccg
     1441 caacacagtt aaccaatact tttacgaata cgatttatta tgatgcaaat gcttcagaca
     1501 ataaatactt taacgaaaat gcgcagaaag ctgtcaatgc gcttattttt gcgttattgg
     1561 aacaagcgga tcaaacaggg gattactcca aattaacacc aaataatctc gttgaactac
     1621 tttcagaaat tggcggtttt aattattatc cagatcctga gaatgaattc aagcagctca
     1681 atgccttaga tgagttcatg aatcagttac cccctggaaa tatagcaaaa aaacaatatg
     1741 gggcaacaaa aattggatct gataaagcga aagggaatat cttgtctacg gcaattacag
     1801 gattgaatcc atttacccta acgaaaattg cgaagatgac ttctcaatcg accattgatt
     1861 acaagtccat tggttttccg aagtatttgg atctcaaatt acatgaaagc ctattaaatc
     1921 aacgcattgt cgtggaattt tatcataagg gaaagaaatt tcacgaggaa cgagtcaaag
     1981 ttggctatcg tggtttttgt gaaatcaact ttgattgtca tttagagcag ggagatcatg
     2041 tttgcttaaa atacaatcat caaggaaaac aatatgttgc ttggtatgag ttttctcaaa
     2101 aagtattgaa agacgaagag ggaaaaatcg tttatcgaaa aaaacgagga gaaactcatt
     2161 tgccagaact agaaaaagaa gccgttctaa cattagatga gaagaaaagt aacctattag
     2221 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg ttaacgcctg
     2281 attatgatcc atctaaccat atcattgtta gcatctttct tagtcagttg tataaagaat
     2341 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgtaggatt cattttattt
     2401 tagatgaatt tggaaatatg ccaccgatgg acaacatgga aggaatcatg acggttacgg
     2461 caggtcgtaa tatgttatgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
     2521 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
     2581 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
     2641 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
     2701 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
     2761 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
     2821 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
     2881 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
     2941 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
     3001 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
     3061 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca acggatcgtc
     3121 ttcaagaata tgttgaaaag ttgatcaaaa atgaagaaat ttctgaaatg attaggaaac
     3181 ggctgttatt ggaagctgaa aaggcggtga aagaatgatc attggaggaa ttttctctgg
     3241 catagccgac tggtggtcag gtttattcgg agaaggggat gcagatccca aaaccgtaac
     3301 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattca gttggatttg
     3361 taatactttt ttatggggat tgatcaaagg gttatataat ctcaatactt tattagaaaa
     3421 aacaatttat caaagctttg atttaaaaga cttattgaac gccgctggtg ttaatgaact
     3481 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
     3541 aggcttgaaa ttttttatga gtaaacaccc accgaaagtc aaaaatatct tggttaattt
     3601 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
     3661 cagtaaaaac ttttatggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
     3721 tgcttttcaa attattcaaa ataatgtgat tgatgtcagt acattactag cgactgaccc
     3781 gaataaagta gaatccttac caacagataa aagaaatgct ttaacagcga aaaatttcaa
     3841 aacagcgaat atcaatggga ttattacacc tgatcaagct aagaaaatgt ctgaatcaag
     3901 tgatattagt acagtacaaa aagatcggtt aaaggcatta cagtatcgac tagagttaga
     3961 cgataatggg aaagaaatcc ctgttcaaat tagtgatgaa ggtttagcaa aatatgtgta
     4021 tacgtcaggc tatcgtcgat acattagtag tccagggatt attttggctg gggaatcttc
     4081 tttgtcggta gcctatttat ttattttgtt tacaatcgtt acttgcatta ttgagctagt
     4141 atttaaaaaa ttctatttag taattgccgc gtcaacggac tttgagacag gacaacggat
     4201 gaaaagtgcg atagaagata tttctcaatc cttcttgtta ttggcgttta cgattttaga
     4261 attacgaatc tatacattaa tgctttcagg tatcggtgat ttgcatgcgg taggaaagat
     4321 caatggattc ttgtatgttg ttgctttaat tgtgctaact attgcgctat tcaaaggctc
     4381 tcaatcggtt acgaagattt tcggtgttga tacgagtctg aaaaacggca gcaattctct
     4441 tcttagcatg tttgccttag gaaatattgc gaaaaatgtg ggtagcggag ctaaaaacct
     4501 tgcaagtgct ggtaagagtg gtttaagcca tttaaatgat attcgtaaga atggatttgg
     4561 ccatcgaaaa gatattaccc atgataacgg ggaagaacaa tcagacgata aagctacaac
     4621 tgtacctaat agtaagaaga cagtttccga aatgttcggg aaagcgaaaa atggtgtttc
     4681 taaagccgca gaaggtcttg gttacgtcaa tgaacgtggg ttaaaaggaa cagttgaaga
     4741 tcttacagaa aaagccaaag acaccttaga agagaaatta gacggcacga gagctaaaga
     4801 atttgctgaa gctgtgaaga atccatccga agcactagaa aaacaaaaag aaaaagcttc
     4861 agcagtcaaa gaaggactaa aagatgcgta tggtgaagga caagtcaatg cagcagtgaa
     4921 ggcaaataca acaagtgaaa aagaaaatca agaaggtgca gcgattcctt cagccaaaga
     4981 tcaccaagat gagttacatt ctaacgaaaa ttcgatcgga gaaaaagatt cagaagaagg
     5041 acaacatggt cagttaccaa aaaccgatcc agaacaatat cgttataaat ctaacattaa
     5101 ttctgaaaac gaaaaacgca tttcagattt accaaaacga aatcaagaag tacaaacgga
     5161 tgaggattcc atcgatctgc caaatgataa tggcatcgaa gttggaactg aaaaatcaac
     5221 gaaaattggt ttgcctaaga cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt
     5281 caaagagaat gatcaaagct ttgttcatca atcacctgca ggtactgttc aaatgaaatc
     5341 aagtcgtttg cctgaaaatg gaaatctgac aactgtttct tacaatttag ctgaaagtgg
     5401 tgaaacagag aatgcgtccg ttcaaaatat tgtgacaaaa gaagctgcac ctaagccaat
     5461 caataaagtt cataatggat acagaccagc aaacgtagca tttactgcac cgacaaaaat
     5521 aaatgtgaca agagaaacta aaaacaggaa caatgaattg aaagaacaac tattgaagga
     5581 tatcaaacca taaaaggcct atttaagggc ctttttcttt ataaaacaag tttaaaatgg
     5641 aaaggagaaa attgtggagg atattttaat agctacgttt ataactgcac tgggagcatt
     5701 tttaacgata tttgtggtga ctattaaagg atttaaagat acggatagtt tacaaaaaga
     5761 tcatcagtct attttagatg gtcaaaaaac acataccgaa agcattattg tacacctttc
     5821 agaaggattc acttatttga aagatgctat gcccacattc acagtaaaat taacggaaat
     5881 atttcattct gttgagcaaa tatcgtcaga attgaaagag caaagacaac aaaaagatcg
     5941 tcaacttggc tcacttaatt ttaggcaaga aacgatacaa gaatcattgg agaatgtaaa
     6001 tcgattaaac gatgaaatgc aacgagttca tttggaaaat gaagaattac gcgcacaaaa
     6061 ttatgattta attcaaaaga aaagaaatct ggaacaaaaa attgttgaat taaaagaaga
     6121 aaatcgtcaa ttgaattatc aattacgacg aggaaaaaat cgttctcgtg atttaggaaa
     6181 tgaattagag ttgtaatatt tagaaaaacg ctgtgagaaa tcacagtgtt ttttatttct
     6241 aaaaatacaa aatataaggg gtatggttat attatggata aatacattca tatgaaaaat
     6301 cctccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga tctattaacg
     6361 cttggtggct gctttttttg ggcgaaaata gtcaataatc ttgtccactt accactcttt
     6421 tttgcactct tgaattatgg attatggctt gcgatagcgt tagtgattgt ttggcgaccc
     6481 tataataacc ctggtcaacg ccaaattttt gcgattatcg aatctatttt ctttacggat
     6541 aagaaccact atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
     6601 gaataaatgc gagtcaaagg acaaaattca gttgataaga aatccatttt gcagtttgga
     6661 aaaccagaag agcgctctat cttggactta tttccatata aatcctgtac agaagaggga
     6721 gtcttaatta ctagagaaga tcattttcag cgttattatc gtgtcgtttc cactgacgtg
     6781 gagggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
     6841 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
     6901 atcgtgcaaa agcggcagtt attagataaa aatcgtatgg aacagtcact aggaagaaat
     6961 ctacagcgat taagaaaata tgaaaagaaa ttagtagagg agatccagga attaaaacgg
     7021 gcagaacaag aacgtcctga cttaagcttc tttttcgtta ttgaaggaaa gacaatcaag
     7081 gatttacaaa ctagaaatcg tcagttaatg cgatctagtg gcattttagg gttaaaacca
     7141 ctctacaaaa aagaattgat taagattctt tatcgaatga acaacatgaa tgacgaataa
     7201 aaaagatgga ggaaatgatt catgctagat gtgataaaaa cagctttttc aacgcaaagt
     7261 aatcaaaagg tgattgatcc attaaaacaa cttggtaatc gtgaaataga gattttaaaa
     7321 tcaaaaggtt atgatttaga tttcttaaaa caagtgatgc cacaaggtgg gttgaatttt
     7381 gatgaagaat atgtgaccta tggaaatggt tattcacgat gtgtgacagt ttatcattac
     7441 acaaaaaatc ctactctttt ttggttggcc aatctcatga acaatcctca tacagtggcc
     7501 acactagatg ttagcacaga tgaaaagcaa aaagtgattg atcaaatcaa ccgcgcatta
     7561 gatgaattaa atgatcgatc agataatgaa cgaaaagcga ctgaccgaaa taaaagtgcg
     7621 gaagaatacc tggaactagc ggaatatgcc cgaaaattga cttctaatgg ggaaatttct
     7681 aaacaagtca aagtgagagt acatgtttac ggaaatacga tccaagaagt ggatgaacgg
     7741 gcgcaggaac tgatcgaaaa tctaaaaggg atggattatc gtgcggtgac ttatctgcat
     7801 aaaaccaaac gagattggca atcgatgttt accatgttcg atgaacaagc tgaatggttt
     7861 ggaaatatca aagggcaatc acttccatcg ttaaacattg gcggaggatt cccgttccac
     7921 caccaaacgc taaaagatcc aggaggcaca caatatgggg aaaccatgac aggcggccca
     7981 tttgtctgga atgccacgta taaaaatagt gtacgtttat ctaccaatac cttgcttcta
     8041 ggaatgatgg gagctggtaa atcgaccgtt cttaaaatga tcgcggaagc ccatttagct
     8101 gcaggagact ttatttgggg ctttgaaaag ggaaaagact ttattccttt cttaaaggaa
     8161 tataacggta ttatggtgcg tttagacgga tctgacggaa tgatcaatcc tttagagata
     8221 tttgccacac ggacgtatga tgaagcatcc agtttatatg acgatggatc agtcaaagat
     8281 ttgaagatca atgaagcagc gtcctatcaa acacacttag acaaagtggt ctatcaggta
     8341 cagttagtga gtcctcagct aaaaggaacg atgaaagcag aatttaagac ctacttaaat
     8401 cgtttttatg aagagtatgg aacagtacca cgcggcttta cttcctcaaa ttcgcgttca
     8461 aatactgata cgcaagtcac agggaaagat ccagaagctt atccgacgtt caaagaattt
     8521 ttagcctttt taggacaact ggaactacca ggggcttccc aagagaaaaa gaatcggaaa
     8581 gaagaaatgg agtctatcgt ggaatcctta tgtgagactt atggcatgat ttttgatggt
     8641 catagtacga ttcgacacct tgaccaacaa cagttggtct tttttgacat tgactctatt
     8701 acaggcctag atcgaggggt acaacaatgt cagatgtaca tggccatgac gttgatttgg
     8761 aatcaggcat tgattcaagg acgaagacaa cgacgcttaa ttaaagaaaa acaaatggaa
     8821 gaacaagact tcaaacgctt cttggcgatg attgatgagt gccataa
//
LOCUS       DAXWLB010000114         8794 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXWLB010000114 DAXWLB010000000
VERSION     DAXWLB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.114"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            33..1094
                     /locus_tag="ACGE4H_002051"
     CDS             33..1094
                     /locus_tag="ACGE4H_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFF8365452.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4H_002052"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ACGE4H_002052"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFF8365453.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ACGE4H_002053"
     CDS             2007..3989
                     /locus_tag="ACGE4H_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFF8365454.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4H_002054"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ACGE4H_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFF8365455.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ACGE4H_002055"
     CDS             4711..6135
                     /locus_tag="ACGE4H_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFF8365456.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ACGE4H_002056"
     CDS             complement(6316..7089)
                     /locus_tag="ACGE4H_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFF8365457.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7086..7991)
                     /locus_tag="ACGE4H_002057"
     CDS             complement(7086..7991)
                     /locus_tag="ACGE4H_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365458.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ACGE4H_002058"
     CDS             complement(8145..8465)
                     /locus_tag="ACGE4H_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFF8365459.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2764 a         1582 c         1873 g         2575 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
     8761 tattatcggg caataaatta aaaaaagctt tacc
//
LOCUS       DAXWLB010000115         8775 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXWLB010000115 DAXWLB010000000
VERSION     DAXWLB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.115"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(476..1063)
                     /locus_tag="ACGE4H_002059"
     CDS             complement(476..1063)
                     /locus_tag="ACGE4H_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365460.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1238..2560
                     /locus_tag="ACGE4H_002060"
     CDS             1238..2560
                     /locus_tag="ACGE4H_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFF8365461.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2715..3491
                     /locus_tag="ACGE4H_002061"
     CDS             2715..3491
                     /locus_tag="ACGE4H_002061"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFF8365462.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3484..4053
                     /gene="rnmV"
                     /locus_tag="ACGE4H_002062"
     CDS             3484..4053
                     /gene="rnmV"
                     /locus_tag="ACGE4H_002062"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFF8365463.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4046..4930
                     /gene="rsmA"
                     /locus_tag="ACGE4H_002063"
     CDS             4046..4930
                     /gene="rsmA"
                     /locus_tag="ACGE4H_002063"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFF8365464.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5381..6010
                     /locus_tag="ACGE4H_002064"
     CDS             5381..6010
                     /locus_tag="ACGE4H_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365465.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6134..7243)
                     /locus_tag="ACGE4H_002065"
     CDS             complement(6134..7243)
                     /locus_tag="ACGE4H_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365466.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7452..7874
                     /gene="mgsA"
                     /locus_tag="ACGE4H_002066"
     CDS             7452..7874
                     /gene="mgsA"
                     /locus_tag="ACGE4H_002066"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFF8365467.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      8061..8159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            8197..8733
                     /locus_tag="ACGE4H_002067"
     CDS             8197..8733
                     /locus_tag="ACGE4H_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFF8365468.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2775 a         1554 c         1796 g         2650 t
ORIGIN      
        1 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
       61 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
      121 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
      181 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
      241 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
      301 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
      361 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
      421 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
      481 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      541 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      601 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      661 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      721 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      781 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      841 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      901 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      961 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
     1021 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
     1081 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
     1141 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
     1201 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
     1261 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
     1321 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
     1381 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1441 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1501 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1561 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1621 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1681 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1741 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1801 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1861 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1921 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1981 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     2041 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     2101 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     2161 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     2221 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     2281 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     2341 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     2401 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2461 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2521 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2581 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2641 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2701 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2761 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2821 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2881 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2941 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     3001 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     3061 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     3121 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     3181 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     3241 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     3301 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     3361 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3421 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3481 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3541 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3601 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3661 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3721 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3781 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3841 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3901 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3961 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     4021 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     4081 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     4141 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     4201 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     4261 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     4321 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     4381 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4441 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4501 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4561 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4621 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4681 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4741 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4801 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4861 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4921 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4981 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     5041 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     5101 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     5161 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     5221 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     5281 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     5341 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     5401 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5461 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5521 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5581 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5641 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5701 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5761 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5821 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5881 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5941 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     6001 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     6061 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     6121 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     6181 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     6241 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     6301 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     6361 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6421 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6481 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6541 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6601 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6661 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6721 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6781 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6841 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6901 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6961 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     7021 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     7081 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     7141 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     7201 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     7261 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     7321 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     7381 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7441 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7501 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7561 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7621 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7681 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7741 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7801 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7861 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7921 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7981 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     8041 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     8101 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     8161 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     8221 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     8281 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     8341 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     8401 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8461 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8521 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8581 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8641 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8701 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8761 gactgcttgt tttta
//
LOCUS       DAXWLB010000116         8742 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXWLB010000116 DAXWLB010000000
VERSION     DAXWLB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.116"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            208..2250
                     /locus_tag="ACGE4H_002068"
     CDS             208..2250
                     /locus_tag="ACGE4H_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365469.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="ACGE4H_002069"
     CDS             2261..2881
                     /locus_tag="ACGE4H_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFF8365470.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="ACGE4H_002070"
     CDS             2893..3351
                     /locus_tag="ACGE4H_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365471.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="ACGE4H_002071"
     CDS             3370..3648
                     /locus_tag="ACGE4H_002071"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8365472.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="ACGE4H_002072"
     CDS             3673..5040
                     /locus_tag="ACGE4H_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8365473.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4H_002073"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="ACGE4H_002073"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFF8365474.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="ACGE4H_002074"
     CDS             6227..6718
                     /locus_tag="ACGE4H_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365475.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="ACGE4H_002075"
     CDS             7161..8360
                     /locus_tag="ACGE4H_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365476.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2715 a         1430 c         1856 g         2741 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcattattct atcttgaaac taagaagtta ta
//
LOCUS       DAXWLB010000117         8719 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXWLB010000117 DAXWLB010000000
VERSION     DAXWLB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.117"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..610)
                     /locus_tag="ACGE4H_002076"
     CDS             complement(<1..610)
                     /locus_tag="ACGE4H_002076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365477.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNK"
     gene            complement(603..1232)
                     /locus_tag="ACGE4H_002077"
     CDS             complement(603..1232)
                     /locus_tag="ACGE4H_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8365478.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1225..2343)
                     /locus_tag="ACGE4H_002078"
     CDS             complement(1225..2343)
                     /locus_tag="ACGE4H_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFF8365479.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2345..2977)
                     /locus_tag="ACGE4H_002079"
     CDS             complement(2345..2977)
                     /locus_tag="ACGE4H_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFF8365480.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2988..4805)
                     /locus_tag="ACGE4H_002080"
     CDS             complement(2988..4805)
                     /locus_tag="ACGE4H_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFF8365481.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4818..5582)
                     /locus_tag="ACGE4H_002081"
     CDS             complement(4818..5582)
                     /locus_tag="ACGE4H_002081"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFF8365482.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5610..6308)
                     /locus_tag="ACGE4H_002082"
     CDS             complement(5610..6308)
                     /locus_tag="ACGE4H_002082"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFF8365483.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6320..7099)
                     /locus_tag="ACGE4H_002083"
     CDS             complement(6320..7099)
                     /locus_tag="ACGE4H_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFF8365484.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7115..8059)
                     /locus_tag="ACGE4H_002084"
     CDS             complement(7115..8059)
                     /locus_tag="ACGE4H_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8365485.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8105..>8719)
                     /locus_tag="ACGE4H_002085"
     CDS             complement(8105..>8719)
                     /locus_tag="ACGE4H_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365486.1"
                     /translation="SKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDI
                     KESELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKE
                     KVTSADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTA
                     TATYDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2355 a         1793 c         1464 g         3107 t
ORIGIN      
        1 ttttattgac tattttctct ccataatcat tttggtcatc tacattgaaa gaacatggat
       61 aaatggtcat attttttgat tctccggaaa tttttatgaa ttttttgaaa taatcgcatt
      121 ctgttataac tagactagaa ttactgagat attgatctcg tagctttttc cataaatcag
      181 taaaaaattt atatttgttc cctattcccg gcattgactc tggccataaa tcatacacat
      241 caaaaataac tcgattgcat ctactaaact tagcaataaa aaaaactaag gtattaggtg
      301 gtattctaca ataaataatt tcaggctttt cttttcgaat aaataataat gtttttattg
      361 aaaaccatat atgagataaa attcgcatta cggatatatt ttttttatat ccgatcgttt
      421 ttatcacatg acgattttta atcatatgcc tttctttact ggatagaaat tgttttctta
      481 tatgtgagta atcagataca acattaaaat attcgatact gttttccgtc agaatttcct
      541 ctaatatatt cgttcgactg atataatcaa aaggatgaat aagcagccac ttttttttat
      601 tctcactcat ctatcttcgc accctcaaat ttctcagtag tcccacttgc tactgatgtt
      661 attccatcac ggacaataac tttctttagc gttaaaaaaa atatttttat gtctttcatc
      721 attgaaatgt tctccacata ttcaatatct ttttcaaatt tttctttcca actaattgca
      781 ttacgaccac tgacttgagc taatccggtt aaacctgggc gcacaaaatg tctttttcgt
      841 tgttcattac tgtatagtga aagatactca actaacagag gacgcggtcc aataatactc
      901 atatctcctt ttaaaatatt ccataattct ggtagttcat caagactagt cgaacgcaat
      961 attttaccaa attgagtcaa tctgatatcg tctgataata aattaccttc ttcatctctt
     1021 tgatctgtca ttgttcgaaa tttatacatc agaaaaatct tttcatgttt tccaggtcgt
     1081 tgttgtttaa agatcactgg cgatccaagc tttattcgaa ctaggattgc aacaaacgtc
     1141 atcagtggcc ataacattat taaacctaaa attgaaaaaa gaatatctat caaacgttta
     1201 aagaactttt cgtagatacc tctattagac attttgcatc actcgcttaa ttgtattagt
     1261 tactcgatac aaatcatcat cggtcatttt agaatctgaa ggtaaacaga tacctttttc
     1321 aaacaatgtc tgagcaacat ctgtaccaat atactcatac ttatcgaaaa ctggctgtaa
     1381 atgcattggt ttccatatcg ggcgggcttc tatattattt ttatctaact catcaataag
     1441 atgattcgta tttattccct ttactgttat gacacttaac caataatttg gttcattcca
     1501 attattttct ggcatgaatt gtatatttga caatttagac aattcttttt tataaaaact
     1561 atgaatatat cgttttttct caattctttc atttaaaact ttcaattgtc ctcgaccgat
     1621 acctgcaacg atattgctca ttcgataatt atagccaatt tcattgtgtt gataatggcg
     1681 tgccttctca cgagattgcg tagcccaaaa cctcactttt tccacctttt ctttgtcatt
     1741 agacacaagc attccgccac ctgaagtcgt gattatctta tttccattaa aagaataaat
     1801 tccatagtcg ccgaatgtac cagtatattt tcctttatat gttgttccta atgattctgc
     1861 agcatcttca attaaaacaa cattatgttc tttgcatatt ttagtaattt catctagttc
     1921 agctgataaa ccgtacaaat gcactactaa tacagctttt gcgttaggat atttttctag
     1981 cgctgttcgc aaatgttcag ggttcatatt ccaagtttcg taatcactat caacaaatac
     2041 tggaatagct ccttgataga tgattggatt ggcagtagct gagaacgtca aagactgaca
     2101 aatcactata tcatctttac cgaccccagc cgcttttaaa gctagatgta tagctgctgt
     2161 tccagaagat agtgcagctg catgactagc accgacatat tctgctaatt cctcttcaaa
     2221 accattgaca ttcataccca aaggagccac ccaatttgta tcaaatgctt cttttacata
     2281 ctcttgttca tatccttcat cactcatatg aggagatgct aataatattc gtttattttc
     2341 caacttaatc ccatctttct atgactttgg taggtacgcc aacattttta gtcttattat
     2401 taatattttt tattactaca cttcccgcac ctataatgtt catatttcca attcttagat
     2461 tttgtattat tttagtacct actccaattt catttttatc tcctaataga acatgtcctg
     2521 aaatatttac acttggaaaa attgaattat aattaccgat ttgagtatca tgtccgatag
     2581 tagagccgat attgatcata ttgaaatttc ctaaaactac gtcagcagtg taagtcgtat
     2641 agggcatcaa aatattgcca attcctaact gaacatttat gcccactagt gcacttgatg
     2701 caactaaatt agggaaaatc aatttattat ttttatttat cttttgatat attttctctt
     2761 tgatatctgg cgaagcgatt cccagaaaaa tagcagtttc ttcttctaat tctaataacg
     2821 agtctattgt tcccaataca gtttttccca ctaattcttg attttgcatt tttaacgaat
     2881 catcgacaaa accaatcaat tcatatctag agaaactttc tagtagaaaa gcaatctctt
     2941 tgccaaatcc accagctcct acaataattg ctttcacttt actcccccta cttattcgaa
     3001 gcattcgcaa aagtgacaat gtctttagcc aattgttcat cgtcttgcgg caggctcttg
     3061 acaaattcca tcactgtttg catggaataa cctttgatgt ttcccacaaa gatcttctcg
     3121 taaactgctt catcgtttct ttccttatct aacaatagtt cttcatatag tttttcccct
     3181 ggacggatac ctgtttcaat gatttcgatc tcgtcttcgc tatatccgct caatcggatc
     3241 atgttttttg ctagatctac gattttcact ggttcactca tatctaaaat aaagatttcg
     3301 ccgccttttg ctaacgcccc agattgaata actaaacgac ttgcttcagg aatcgtcatg
     3361 aaatatcggg tcatacgaaa gtctgttaca gtgactggcc ctccttgagc aatttgttcc
     3421 cggaatactg gaatcacact accgcgagaa ccaaggacat tcccaaatct gacagctgag
     3481 aatttcgtac atccttcttc attcaaccca gtcacgatca tttcagcgat tcgttttgtt
     3541 gaacccatga cgtttggcgg attgttggct ttatctgtcg agaccatgac gaagtttttt
     3601 actttcgctg ctttggctgc ttctgctaca ttttttgtac cgaaaatatt gtttttgact
     3661 gcttcttttg ggttatattc catcaatggt acatgcttat gagcggctgc atggtagacg
     3721 atatctgggt ggtattgttc catgatctca ttgattttct tgcgatcttg gatatccgca
     3781 atgatcggca cgatttctgt aggacacttt tgatgatgcg tgcgtaattc acggtcaatc
     3841 aagtaaatcg agttctcccc atgacctaaa agtaatagtt ttgcagggtc aaattggatg
     3901 atttgccggc agatttctga accgattgat cctcctgcac cggtgacaag aatgactttg
     3961 tttgtgatct ggtctttgat ggattcgata tccaactcta cctcatctct tcccagtaat
     4021 tccactacgt cgatggtttt taattttgat acgctgattt tccctgaggc aagttcttcc
     4081 atagaaggca tcgtattgac tttcacatga gcagattcga ctaactcgaa gattcttcgt
     4141 aatttttttc ttgataaaga agggatagcg atcgttacca tttggatgtc atatttttcg
     4201 atcaattccg gtatatcttt taatgacccc agtacttttt ttcctgaaag ataggtattt
     4261 cttttattcg gatcatcatc cacgaatcca atgacatgga tatcttgagc cgttttagaa
     4321 ccaagaaagc tgttgtataa aatacgtcca ccttcgccgg ccccaacgat caaggtattt
     4381 ttagcggaat ctgttgatag atacctcata ttttttgttt cgatatagat acgccagatc
     4441 aatcggctgc caatgatcag taatagcgaa agtaggtatg caaagatcac taaacgtaaa
     4501 ctgtattgtt tatttatgaa aagcaatatg atgatacttg aggccgcact tgcagataac
     4561 gaaccaaaaa tcgcaatgat ctctcttaaa tttgtataac gattgattcg agtaaaaact
     4621 ttgaataaac taccatagaa taaataaaaa ccaatcgaca aaccgataga gattaaaata
     4681 aggtccattg gtatagctac aaacggcttc atgaatttag ctgctgcaat attcgcagca
     4741 atcaacaaca agctgtccac aaataccaat gtggcaattt ttcttcttct tgttaataca
     4801 aacaccctac attcccccta aaataaacca aacttcttct tttttacctc ttcatagact
     4861 ggataactga tctcatcccc gttgatcagg tcctttgcga cttgtttcat ctgtgctact
     4921 ttttccttac caaagtcctt ggcgatttgc tcataacatt cttttaagta aaaggtacgt
     4981 ttagagaccc catgggcatc ggaagcagcc atttgcacga ggttatgttt gaccatcatt
     5041 ttggctgttt tctggatgtc ttttccaaat acaccgacat aacttggtgc ggtgagttga
     5101 gccaagcatc ccatatctag gaatggcagc aaatgatttg ggtcttctcg aaaatgcgcg
     5161 ttgcgttctg gatggacgat cactggtgtc tttcctagtt gtaacaactc aaaaaagagc
     5221 cattctgcat ataacggcac ttccagtgtc ggaaattcga ttagaagata tgtatcatct
     5281 aaatctgtaa ataaaatttc atctcggtgg atgtcttcaa tcagtgttcc ggtaatacgt
     5341 acttcttgtc cttccaataa cgtcaaaggc aattgtcttt tctctaattc tgcttgtaac
     5401 gaagcgacta gagaaatgac ttgtgatttt tcattgctgt attttccatt attgtgatgg
     5461 ggtgtgcata aaatatgtgt gatcccttgt tgaatcgctt tttcagccat ggcgatactg
     5521 gcatctatgt tttctgctcc gtcatcgatt cctggcagga tatggcaatg tagatcgatc
     5581 atttctcttg tctcctctct ttttatttct taattcccgt agtaatagcc agcatctctg
     5641 ctgtgttctg ctccattgta tacaacacca aggactcgcg cttgtaccat atctaacagt
     5701 tcttttgctt tagtcaatga ttcttttcgt gacgtatttt cccgtacgac caataaggtg
     5761 ccatccgttt ttgaagacat gatctgtgca tctgttacgg caacgactgg tggcatgtcg
     5821 aaaatcacta catcaaatag ttgtcttgct tctgctaaaa tctgatccat tcttggcgaa
     5881 cttagtagtt ctgatggatt tggtggttta ggtccacttg gcagaatcga caaattgtcg
     5941 actggtgttc gttggatctc atctgctaca cttcctgatg aactaagaac agtacttaat
     6001 ccgcttgcgt tgttcaactt gaacgtttta tagatgacgg gcttacgtaa gtctgcatcg
     6061 actagcagta cccgttgacc agatttggca aaaacaacgg caatgtttgc tgcagtggtg
     6121 gattttcctt ctccaggacc tgatgaagtc acgacaatcg ttttgatttg ctgaccagct
     6181 gcggaggcaa actggatatt cgtccggatc gtacgatatt gttcggcgat tggtgaagat
     6241 gggtttgtta atgtgatcaa actgactgca tgagtttgag ttgctttttg tttctgtgtt
     6301 cgtgccattt ttcttcctcc tagactcttg agcggcttct gcgtgatggg gctgttttat
     6361 cgtttgtagc ttttggtcgt gcaggagctg atcctttttt acggatacca gcgttcaatt
     6421 cttttgctga catttctgga actgttccta agattgggaa tcctaatgta tccgtcacat
     6481 atttttcatc tttcactgtc cgatcaagta attcgaataa gaatgccaga cctacgccta
     6541 ccatcattcc taacactaag ccaatcacta gattcaattt attatttggc gaaacaggtg
     6601 tcgtattggc aactgcacct gaaataatcg agattttatc gacattcatc acgtctttgg
     6661 cgttttcttg gaaaacttgt gaagttgtat tagcgatctg ttgagcagtc actgcattcg
     6721 tgcttattgc ctggatcgag aacatttgtg aattttgtga ctgattgacg gagatcgcat
     6781 ctttgatttg tccagcagtc attttcacat tgtcttctgt ttctaaacga tctttgactt
     6841 catttagtac taaatcactg acgatcatat ctttatacgt attgatcatt tgcaggttcg
     6901 tattgacatc attgacattt gctgtgtctg attgtggcag tgtcacgatc aattgtgctt
     6961 gtgaactgta ttttggtgtc aagatgaaaa atgttacgat tcctgagatg gccaagccag
     7021 caaacattga gatgatgatt gttaccaagt gtttgcgtaa tgtttcaaaa atttctttca
     7081 agctgattgt ctcttccatt ttttctccac tttcctattt attctctaga ttcatttgat
     7141 cttttaattc ttttttcaca cgatcaagtt cttgatcgct gactttttga taggagatat
     7201 tatcttgcat gaagccttct ccttgcagct gatcttgctt gatcgtacca aatgcctccc
     7261 gataatctaa agcgattttt ttcatttcat caaagctcat gtctgttttc atattcgact
     7321 ctagagcatt caaaacggat tgatagttgc tgacaccgtt taaactcaat actttttttg
     7381 ctacaccttc tacgatcttt ctttgacgct cttgtctacc atagtcacca tttggatctt
     7441 cataacgcat acgagaataa gataatgcct gatccccgtt caatgtaatt ttaccgattg
     7501 gaaagtcata gccatcttgt gagaaagtga ggtcattatt gacttcgata ccaccaactg
     7561 catcaacgag ttcctttagc cctttcatat tgatagagac atagtgatcg attgggatgt
     7621 ctacatattt ttgtagggta tccatcgcca tggcagctcc gccgaatgcg taagcgtggt
     7681 tgatcttgtc ttttgtgtca cgacctacga tgtcgacata cgtatctcga ggaatgctga
     7741 cgatcgttgt ctgtttgtct tcaggactga ctgtggcaac catcatcgta tctgatcttc
     7801 cttgatcgac ccgacctaaa tctccagtat caatgcccat caatagaaca gagaaagatt
     7861 cttgtttgtc cagatcaact ggtttcgttc gttctccttg ggttcgttcc actgattcgt
     7921 aagttttctc aatagaatgt gagagatcca gatataattt tgctcctact cctacaattg
     7981 ctaatactaa tacgataaga actgctaaaa taatcagaaa tatttttttc cctcttgatt
     8041 ttcttcttcg tcttgccata gtttcctccg tttgctaacc ttgcttgaat caacgtttta
     8101 actgtcaata acttatattg ctatagccca tttgctgagc aacagtttgt gcttttgttg
     8161 cgtatgctgc gcttattttt tcattccgga tctgctctat ttgactgagc gccgtcaaat
     8221 attgatcgta agtagcggtg gcagtcactt gaccatcctt caacatcgta tcgaataact
     8281 gatctagttg cgtgacttgc gcgatctgag tatcagcgaa tcctagatat tgcataacaa
     8341 ccgttttcca aggactatct ttaaaaaagc tcatattttc acgtacacga gcgacatctg
     8401 cacttgtcac cttttctttg atgacgacat cgtctacagc ttgctgccag tttgatacat
     8461 ttttagtaaa taaatcgttg acagctgtct ggatcttcag ttttgattct gcactatcta
     8521 actgtgtcac gattgctttc ttctcatctt ggattgcaac agcttcttta ggcaattctg
     8581 attctttgat gtcgtaatca tctgctgaaa gtttgacaga ggatagctta tttgacaaac
     8641 ttgtgattgt ttcttctgtt atttcagggt ttagaaaatt tttttctttt gtactataca
     8701 gcttgtctac ttcttttga
//
LOCUS       DAXWLB010000118         8679 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXWLB010000118 DAXWLB010000000
VERSION     DAXWLB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.118"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            2..74
                     /locus_tag="ACGE4H_002086"
     tRNA            2..74
                     /locus_tag="ACGE4H_002086"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACGE4H_002087"
     tRNA            78..150
                     /locus_tag="ACGE4H_002087"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACGE4H_002088"
     tRNA            155..225
                     /locus_tag="ACGE4H_002088"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACGE4H_002089"
     tRNA            238..311
                     /locus_tag="ACGE4H_002089"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACGE4H_002090"
     tRNA            323..394
                     /locus_tag="ACGE4H_002090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACGE4H_002091"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACGE4H_002091"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFF8365487.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACGE4H_002092"
     CDS             1983..3455
                     /locus_tag="ACGE4H_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFF8365488.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACGE4H_002093"
     CDS             3538..4326
                     /locus_tag="ACGE4H_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8365489.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACGE4H_002094"
     CDS             complement(4393..5061)
                     /locus_tag="ACGE4H_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFF8365490.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACGE4H_002095"
     CDS             5176..6888
                     /locus_tag="ACGE4H_002095"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365491.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACGE4H_002096"
     CDS             6891..8663
                     /locus_tag="ACGE4H_002096"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365492.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2611 a         1484 c         1933 g         2651 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgt
//
LOCUS       DAXWLB010000119         8676 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXWLB010000119 DAXWLB010000000
VERSION     DAXWLB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.119"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGE4H_002097"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACGE4H_002097"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFF8365493.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACGE4H_002098"
     CDS             complement(1098..2201)
                     /locus_tag="ACGE4H_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8365494.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGE4H_002099"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACGE4H_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFF8365495.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACGE4H_002100"
     CDS             3528..5462
                     /locus_tag="ACGE4H_002100"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8365496.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACGE4H_002101"
     CDS             5919..6335
                     /locus_tag="ACGE4H_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFF8365497.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACGE4H_002102"
     CDS             6349..6915
                     /locus_tag="ACGE4H_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFF8365498.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACGE4H_002103"
     CDS             6938..7924
                     /locus_tag="ACGE4H_002103"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFF8365499.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8676)
                     /gene="scm"
                     /locus_tag="ACGE4H_002104"
     CDS             complement(8079..>8676)
                     /gene="scm"
                     /locus_tag="ACGE4H_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8365500.1"
                     /translation="SDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTS
                     STTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSK
                     RALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2538 a         1674 c         1771 g         2693 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgtgtccgtg ctatcacttg tggtagaggt agtatctgta
     8641 ctgtcgcttg aagctgacgt gtccgtgctg tcactt
//
LOCUS       DAXWLB010000120         8563 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXWLB010000120 DAXWLB010000000
VERSION     DAXWLB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.120"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(53..910)
                     /locus_tag="ACGE4H_002105"
     CDS             complement(53..910)
                     /locus_tag="ACGE4H_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8365501.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(895..3597)
                     /locus_tag="ACGE4H_002106"
     CDS             complement(895..3597)
                     /locus_tag="ACGE4H_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFF8365502.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(3610..4899)
                     /locus_tag="ACGE4H_002107"
     CDS             complement(3610..4899)
                     /locus_tag="ACGE4H_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFF8365503.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            5049..6095
                     /locus_tag="ACGE4H_002108"
     CDS             5049..6095
                     /locus_tag="ACGE4H_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365504.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            6097..8250
                     /locus_tag="ACGE4H_002109"
     CDS             6097..8250
                     /locus_tag="ACGE4H_002109"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFF8365505.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIRQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         2397 a         1773 c         1556 g         2837 t
ORIGIN      
        1 tcttatcagc agataatgat aagactctgt tctttttgtg taatttgtct gcttactttt
       61 gatgttcgtg ttctgctaac agcaaactac cgataatccc actttcattc ggcagtcccc
      121 aacgaacgat atattcactt acttgaggtg tttccatata accacccatc aattcaacaa
      181 attgctgacg gattttttga aggagatgat cctgattcat taccccaccg cctaaaataa
      241 tcttttcagg tgctaacgtc aacgtatagt tgatcagtgc ttgagcaata taataagcct
      301 gaatagccca aacaggatga tcttgcggca gatcttgccc tttgatccct gtgcgtgcct
      361 caatagacgg tcctgctgct aatccttcta aacaatcttt atgataagga caagtacctt
      421 cataatggtc atctggatgg cgtttcaccc aaatatgtcc catttcagga tgtgcgatcc
      481 ctgaaaaaat ttcttcattg atcacgacac ctgcaccgat tcctgttccc acggtaaggt
      541 aaatacaact attttttcct tgtgctgctc cttgcttcaa ttccccatat gcagcagcat
      601 tgacatccgt tgtccaagca gcaggaatat tgtagcgttc cttgattgtg ccaagaaaat
      661 cgaattgtgc ccaccctttt ttgggagttg ccaaaacgta tccgtaatta tcttttgttt
      721 tgtcgatacc aatcgggccg aatgagccaa tcccgattcc cttcaattca taacgatcaa
      781 aaaattcaaa tacctgatcc aatgtttctt ctggtgttgt ggtagggata cttaattttt
      841 ctaaaatttc tccaccatct gaaaccgcac acacaaattt tgttccgcct gcttctattc
      901 cgccatacat tcgttttcct cctcgaatcc gattgtccga atctcataag gttggattat
      961 tgggacaacc gcttgcttcg ttggttcttc aagcagattc aatatgacag tttttccatt
     1021 gtctttagtt atttccaatt tttctaaatg actagacata ttgaaacctc ttactactac
     1081 tttatcacta aacttgctgc gtttcaaagc tgtaattgca aacgtttcac tttttatcgt
     1141 taaatattgt tgttttgaaa tcaatgttcc ttcatgatgt ttgatttgtt gagtggaaaa
     1201 aggaatctgt gctgcgtatg catgcaaata agtagagaat ttttcttctg gtccatgtag
     1261 ttcaatgctg taattgaacc gatgctcacc tagacactgt gcttctggtg ttggaaaata
     1321 tccccaatcg cctagttctc ccacactgcg aagtagcgtc actgcgattt gtccgtcttc
     1381 tgttacttca tattcattca aaccaaagtt tccgactgtt acaccgtact cttcatcatg
     1441 aatattgaca aatgcttgct gatgttgcgg attcgtgggg ttttcccaac ttttagacac
     1501 atggtttggg cgcttcacca cttcaaaaat actatctgct tcgtgatgtt ccacatgtaa
     1561 ctttgtagga aacaagacac gtaagcgatg atctttcatc tgattgtcga gagtcgtttc
     1621 aaagtcgatt tttttactat cttttctgat tgtcatgatt gtttttattt gaaggattcg
     1681 tttttctttg gatcgttctg cttttcgata acgaaattcc acaaccatct gctgttcttt
     1741 ttctaaacgc tcgtctgcag aaacagggat ttccatttcc tgtagaatac ttatttttgc
     1801 tatttccggt gtatctacta atacttttac ttcagagttt tctttgttac tggataggat
     1861 tggctgatca cagaaaggtt gtttaaagat atactcattt cctatatcgc cgatatcttc
     1921 aaaaatatgc agattattca actttcgttt gtttttgcga tcttccattg taatggtacc
     1981 atttttttca atcgtcaaat gtagagggcc gttttcgatg attcgtccac tctgatgaat
     2041 cattgtttcc ttttcttctg ctttcgcttc cccttctaca agcgcgaatg tttcccaaga
     2101 aaatgctggc atgttttcta atggtaaagt cactttgatc attcttgcca tatatggcac
     2161 acggaaacgg tctttcggca aatcataacc aaattggaca gtttcttcag ataaaatagc
     2221 tggtatagtg gccccacttt ttgtttctac atgatactta cgtttaggca aattttctaa
     2281 ctcttgatat agttgttcag gtattccttc agcgaatctt tttctttcta attcgatggt
     2341 tatttctgct tcaccagttt tatccattcc tgcagtattt acaatgacaa atggaaagct
     2401 gtcttttggg aatccggtcg tatcgattgc tgcggtcagc tgttctaagg aatcttgtgc
     2461 aagatacttt ccaacttcat ctgctttttc aaaacgaggt atcatttccc gatggacaga
     2521 atcgactgaa caaccgcaaa tactgtcgtg aggatgattt tgcattaatg tcttccaagc
     2581 ataatctaat tgatcatggg gatagtttcc actaacttca taagccatcg tcgcaagtgg
     2641 ttctgtaata ttttctagct gtcgctggac cttggtattc cattgtttta agtaaacccg
     2701 agcagatgct gtattggcta aagtatacca gccatccgtt tcttgacttg tcaattctcc
     2761 ttctactgag cctaagtctt ctggcacatc tttttgaact gcttccagat agtcagtaaa
     2821 attagaatga ataaactcgt aatctggaaa cagttcattt gcgagatgga ttgcttttga
     2881 tatatctttt tgtaccggtt gatgatcaac accattcatc atcaataagt ggttcgtaga
     2941 ggcatattgt tctgcatccg ctaatttttg cttccaaaag gcaagagctg cttctttttc
     3001 tactggaatc tcattcccgt tgctatacca attggcaaat agcagaccaa agattgctgt
     3061 ttgatcaggt cctttccacc acatctctga gtattgggaa gaatagtttt ctgcttccaa
     3121 aacttgatta tcaaagccaa ttggttttac accacggcca aacgctgctg ctgaaatacc
     3181 agcttgtttc attaattgag gcgtctgtcc catatttcca aatgtatcgg gaaaatagcc
     3241 taacatgaca ggggttcccc atttacggct ttcttccatg ccaatcagca tatttctcac
     3301 gttcgattcc gagctgataa gaaaatcatc ttgcaagata taaaatggtc caattctcaa
     3361 ttttccttca ttgattgctt gttggattgc ctgtcttttt tctggcctta cttgaagata
     3421 gtcatctaaa ataatcgttt gcccatccaa atgaaaactg ttgaaggacg gatcaacttc
     3481 aaatagctct agtagatcgt ctatcaattc aatcagtcgc atgtgatgct gttcgtaagc
     3541 catgtaccat tcacgatccc agtggctatg agaaatgata tatactttct ttttcattta
     3601 cgatacctcc tacttttcta ctctaatatc aaagtaatcc atgactaatt cgcaaaacat
     3661 catatttgcc caagaaaacc attctctcgt ataattttca ggattgttca catcgaatcc
     3721 ttcgtgcata aggtgtgttc ctgcgtcagt tgctacaagt aaattcaata tccgctcttt
     3781 ttcagatttg tctgacgttg tcattccttc catagctaat gcaatcggcc aaacgtattt
     3841 ttctggcgta tgggaggatc caattccttt tgcgtacttt ccttcataga aatatggatt
     3901 ttctttactc aacaatgttc ttcttgtttg cagataatct tcctcttgtt cagaacaata
     3961 tcccaagtaa ggtgcggcga tcagattggg cacattactg tcatccatga ctgtggcgtt
     4021 tcccattcca tctacttcat atgcataaat cgtttgccct ttttgattaa ccgttcgtcc
     4081 gtgattctga atcccctctt ggatctcttt cttcaatttc tgtgtttgct gtacaatttc
     4141 tttatcatcg agtatcgttt gaaaaatttc ttccacataa cctaacacga ccaccgcaaa
     4201 catattggat ggtaccagat atccataacg gcaagtatca tcgctaggac ggaaaccaga
     4261 ccatgtcatt cctgtaaaag ctgtttttgc acctcgcccg tcattgatca gcgtatcttc
     4321 ttttcttgtc gtgtctcgta caaatgtata aggagagtgt tcatgatctt gctctgtttt
     4381 gaacacgtcc aagatttttt tgataccttc aataaaagat tgattgaatt gatcagtttt
     4441 tcctgtattt ttgtaaagaa gataggccaa ctgtattggg tagcataaag aatcaatttc
     4501 atattttctt tcccagatcc aatcgttcat ttttgtatga tctgtttggt ggccagcacc
     4561 gttagcttct tcgttgaagg cattcgcata tggatcgata ttgatataaa aaaattgctt
     4621 tttgactaac ccgctgatca tgtcagctaa ttcctcatct tcttttgcaa tcactaaata
     4681 aggacgaact tgtgcagtag agtctcttaa ccacatcgct ggaatgtctc ccgtcaataa
     4741 aaaggtggtt ccgtcatttt gctttttgac tgttgtcaac agagtattgg caaatgctgc
     4801 tgtaaaattc tttgcccagt ccgcatggat atccccacat ttttgtgtga tatcttccat
     4861 aaatttctgc accgattttg gtatatcttt ataagccact aataaaacct tctttctatt
     4921 tgatatatct ttaaagatta tacttcatac agccaattat gtctacgctt tcaaaaaata
     4981 tctttttatt tactttttca tccattatgg tattatttga atatgatata tcattaagag
     5041 gtgtttatat gaatcaacca ttgtaccaga caatatttaa tgatttgcaa acggctattt
     5101 taactggtga attacctgta gacagccaac taccaactga aaaagaactt tcacgtacct
     5161 ataaagtaag cagaatcact tccaaacgtg cattgactga attagagcaa ttaggactta
     5221 tttatcgcgt tcgcgggaaa ggcagtttcg ttaaatccgc ttctccggaa cagattcttg
     5281 cttctcctac aaaaacacga cggattctgt ttcttcttcc atatgtcgct gatttgtctg
     5341 ttggtgattt tagcaaggga ttgaaccctg tgatgcaaga taaacagatt gatgtcatga
     5401 tgacgacgct tgactttcta caaaataaaa cagcaaaaga gctaatcaat gaatttgacg
     5461 gattgattta ttatgctctt gatacagaac aacatcttga cttacttttt gagctttcat
     5521 tgaaagaatt ccctgtcgtc atattagata aaaaaatcta tgagttacct tttcctaccg
     5581 ttctttccga taatttccaa ggcggtgcgc ttgctgcctc ttatttgatc gaacagggac
     5641 attcaaaaat cgcttatttg tttggtacgc agactcatcc acaatccgtt cgacagcgtt
     5701 accttggtta ccttgaagca ttggacaaag ctggtttgac tttccacact tcactggatg
     5761 acaaaagcgc gacattagat gaattgcttt cttatatcac taagtatgaa gtaactgctt
     5821 ttgtttgtga aaacgatgtt gtcgctatcc agaccatgaa tctcttgaga cagcatggtt
     5881 atcatatccc tgaagatttt tcaattatcg gatttgatga tatccaagca gcagctcttg
     5941 ttgacccgcc actgacaacc atcgcacaag attttgaaca gttaggtaag cttgcgggag
     6001 atgctttggt cacttggtta gagacaaaag atcgaccatc cgatagtaaa tttccagttt
     6061 cattcatcaa aagacaatca acaaaggaga aataaaatga atatcggtca aatagatact
     6121 agacatgcaa cagcgaatca gccaaatttc tctaatggga actgccagcc ttatactggc
     6181 gttccttttg ggatgaatta ttttgcaccg caaactacgg atcaaaacgg cagctggtgg
     6241 tttcatcctg atgaccgcgt ttttcaaggc taccgtctta cccatcaacc aagtccttgg
     6301 atgggtgatt tcagtcatat gctgtttacg ccgatcaatg gaaaactaca ggagaacacc
     6361 cttttccatg ctcaaagttc ttaccgccca gaagaaagta tattttgccc gactcatctg
     6421 tcgatctgtc aattacgtta tggcatctgt tctacattga tacctagcat gtatggcgga
     6481 atcttatcta ttgattacag caaaaatgac agcggtttac ttttatcctt tccgggacgc
     6541 catcagctct ttgttataga tccttacaca gtggaaggaa aaatcacaca ttttgctggt
     6601 tcagaagatc ctgatttttc tttttatttt atattgagat ttgaatatcc tttaacaatt
     6661 gatgagttag ctgtttccaa caaaaatagc gactcgatcc cgttgtattt tgaacagaca
     6721 aaaaaacaaa ctatccgatt tgggacatct tttatcagct cggaacaagc agcttataac
     6781 ctggaactag aacaaaacca aagcaaagag acttaccttg ctcacagtca agaacaatgg
     6841 cagtcctatt ttgatcgtat acagctcaca caccatgatc aacaacaatt gcggacgttt
     6901 tatcataatt tatatcgggt gtttttattt ccacaaacat tttatgaaat tgatcctagt
     6961 gggcaaaaaa tccactatga tacgactagt cgatctataa aacctggtgt tctctataca
     7021 aataatgggt tttgggatac gtataaaacg gtttatcctc tttattcttt gattgctgta
     7081 gaaaaatacg aagaaatgct ggaaggtttt ttgaacagct atagagaaag tggctatttg
     7141 ccaaaatggc tgtctcctga tgaacgtgga cttatgcctg ggacattgat cgatgcggtc
     7201 attgcagatg ctgctgtaaa agatatccga cgagacttga tgcctgaatt tctaacggca
     7261 atggaaaaag gtgctacggt tcaaagcaaa catccaaatt atggacgcca aggaacagaa
     7321 gattacttga catatggcta tgttcctaat cactaccacg aatcagtcaa tcacacatta
     7381 gactatgctt atagcgattt ttgtattagc caagttgcca aggtactaga agaagaggaa
     7441 atgacgaaga aatatcagca gcaatcattg aactatcaaa atatctttga ctatgagtca
     7501 ggatttatgc gttcaaaaga taaagatggc aagttcagag aaccattttc agctaatcgt
     7561 tggggacaag attatgcaga aggaagtgct tggcaaagca gctttgctgt ttatcacgat
     7621 ttttcaggac tgatcggaaa atatggtggc agtgagagat ataaagaaaa actcattgag
     7681 ctgtgcaatc agcccccaac gtttgaccca ggaggatatg gttttgagat ccacgaaatg
     7741 agtgagatgg ccgcgataga atttggacaa ttagctattt caaaccagcc aagttttcac
     7801 tatccttact tgttcagtta tataagacaa cctgaaatgg cacagccact gcttaaacag
     7861 ttgatgactc agaccttcga cgattctcca atgggttacc ctggagatga ggacaatgga
     7921 agtatggccg cttggtatat cttcaatagt ctgggatttt atcctgtcac gcctggaacc
     7981 ggtgaatatg ttatcggtat gccattaatt gacaaggctg ttttgctttt gtcaaatgga
     8041 aaggaattga cgattgaagc aacgcctaat cggccgcagc aacaatttat ccataaaatc
     8101 aaacggatgg acaaagaata tacgaaatta tattttaccc atcaagattt gctgaaaggc
     8161 ggcacgatct cttaccaatt agggatcatc cctcacccta agaaatatca agcaaaagat
     8221 ttgccttttt ccttatcaaa ctataattaa caagaggttg tgaaaaagct gtcctgcatc
     8281 aagtaataag aacagccaag caaaaataga tcctcatatt tttcgtttgg ttggtcttat
     8341 taccgcagat gcaagctttt gaacaccgtt tattgagagg ttgtgaaatc agcgttctga
     8401 cctgagtatt agagagacaa atggcaaaat agttcctcat attttgccat tttttgagct
     8461 aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtgaaa aagctgttta
     8521 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc aca
//
LOCUS       DAXWLB010000121         8338 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXWLB010000121 DAXWLB010000000
VERSION     DAXWLB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.121"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            308..916
                     /locus_tag="ACGE4H_002110"
     CDS             308..916
                     /locus_tag="ACGE4H_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8365506.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1084..2721
                     /locus_tag="ACGE4H_002111"
     CDS             1084..2721
                     /locus_tag="ACGE4H_002111"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFF8365507.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2793..3905)
                     /locus_tag="ACGE4H_002112"
     CDS             complement(2793..3905)
                     /locus_tag="ACGE4H_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8365508.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3980..5353)
                     /locus_tag="ACGE4H_002113"
     CDS             complement(3980..5353)
                     /locus_tag="ACGE4H_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365509.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5574..6191
                     /locus_tag="ACGE4H_002114"
     CDS             5574..6191
                     /locus_tag="ACGE4H_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFF8365510.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6289..6744
                     /gene="argR"
                     /locus_tag="ACGE4H_002115"
     CDS             6289..6744
                     /gene="argR"
                     /locus_tag="ACGE4H_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFF8365511.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6762..8264
                     /locus_tag="ACGE4H_002116"
     CDS             6762..8264
                     /locus_tag="ACGE4H_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFF8365512.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
BASE COUNT         2865 a         1560 c         1707 g         2206 t
ORIGIN      
        1 tttttttaag agaatttgat aaaaatcaaa aatttttttg ctttttttta tggattattg
       61 cagtgaatgt tacagaatct gcgtttttca ttcaaaaata aaaaaacata gaaaaacgat
      121 tactttgctt atacaacaag gcttttatgg ttttgtatca aaaaaatatt tgtttaaatg
      181 ttacaaatat taaataattc gttattttgt cattaaatgt catgtttctt ttacctatga
      241 aatatttcta aatgataaag ttaatagcgt cgaaagaata aaaaacggag aaaaaaagga
      301 gtttaatatg aattcattta agaaaattgt tttaggtaca acttttgctg ctggtgctac
      361 tgcaatgttt gtaggaacta caaatgctca cgctgatgaa gtttatacag taaaatcagg
      421 tgattcatta tctaagatct cacaaaaatt tgcaggagac aactcaatga tcgatgcaat
      481 tgctgaaaaa aattcgatcg caaacatcaa ccgcatttat gttggtgaac aattaacaat
      541 cccaacttca aatgattctt cagcaacaac agaaaacaaa acaacagaaa atacagcatc
      601 tacaactgaa acagcaacgc aagaacatac atacgttgca cctgttgaaa cagttgaagt
      661 agctcctgct gccccagctg ctgcaacagc gccaacttca agctctgcaa aagaatggat
      721 cgcacaaaaa gaatcaggcg gttcatacac agctactaac ggacgttaca tcggccgtta
      781 tcaattagat gcttcatact taaacggcga ctattcagct gccaatcaag aacgtgtagc
      841 agaacaatat gtaacatctc gttatggttc atgggatgct gctaaaacat tctggttagc
      901 aaacggctgg tactaagccg ataaaaggaa aatgatgaaa ccgagaaact tgtttattca
      961 agttctcggt ttttttgtgt gaaaaataac tcgtttgctt cccacagcgc agcagtacaa
     1021 ataaaaactc gaataatgta taataaagaa aacgtttttt tgtttaggga atgaggagga
     1081 gccatgagta caaaagagag tctcgaatta aacgaaatca caagtggaaa tccactgtta
     1141 tccagtattc gatcgattgt agaaacgaca ttttacggta ataatgttca tgaagttttt
     1201 gataggaaga cagcttatca actagcaaaa gggagtccag gaacgatcat cacagattta
     1261 acaatctcac atgctgaaga actagattta ccagctgatg ttaggacact agtattcaat
     1321 gatggaagca ttgtgggaag aacagcaagt gctcggcgga tctttgagga cttagacaag
     1381 gaacaaagca aatacgagaa aattttaaga gaagctgtgt atcaaagtcg caagcgccag
     1441 ttttaccata caaaagtaat cgttggccta tctgaagaat tttctgtgca aagtcatttg
     1501 ctgattccag aaggatttga gaacaatttg tattcttatt tgctgaattt tcagccagcg
     1561 aattcgaaga tcttagagac ttatcaaaag tctgtttcct ttgatgaggg agatctttat
     1621 atcttttgtg atcccgaatg gacacatgaa acatttccaa atggactcat tttgtttgat
     1681 gctctccata atacagcggt tgtgctagga ttacgttact ttggagaatt aaaaaaagcg
     1741 actttgacgc ttgcttgggc attgggaaat cgcaatggct ttattgcctg ccacggtggg
     1801 atgaaacaat atgctagtaa tgaccagacc tatacgatgg ctgtatttgg gttgtccggt
     1861 tctggaaaat caacgattac cttagcaaaa catacggaag gtcactctgt ttcgatcttg
     1921 catgacgatg ctttcgttat ttctcgaaaa aatggttttg ctactgcctt ggaaccttct
     1981 tactttgata aaacacaaga ctatccgtct gatgatccgg ccatttcttc tttccttact
     2041 tgtcagaatg tgggcgtgac atgtacaagt aaaggagaaa aagtcctagt aacaggagat
     2101 atacgaaacg gaaatgggcg gacagtcaaa tcacgttatg cgacacccga ccgagtcgat
     2161 catcttagcg aaaagatcga tgctgttttt tggatcatga aagatgattc gcttccgcca
     2221 attatcaaga tcaatgaccc agttttagca gcgtcttttg gtgcaacatt agcaacaaaa
     2281 cgatcagacg cggaaaatat cactacaaaa agacagctag atgagctagt cattgaacca
     2341 tttgctaatc ctttccgtgt ctatcctctt agggaagact atcaagcttt tcgagagttg
     2401 tttttgaaac agcaaacagc ctgctatgtc ctgaatactg gatatttcga tgataaaaaa
     2461 atcaaacctg aaacgacatt gtcaatcgtt gaaagaatcg tgcaacaaac ggcagaattc
     2521 aagtcgtttg gtccatttga agacatccac tatttaccta taccagattt tcctgtttct
     2581 tttgaggata cgtcctataa aaatctggtg aaacaacgac ttgatattcg tttacgattc
     2641 attgaagctc agataggaaa agatctcttg ccagatgaag caaaagaaag tttggagaat
     2701 ttgattcaga aattgaacta aaataacagc tttttcaaga agtaatccat gcgcaatatc
     2761 gctcgggatc cttacttgaa aaagctgttt ttctatgatg gatttttcgt aatataggtg
     2821 gagatgtcct tttgataatc ttccgtaggc caatgaccag tatttaagaa gttggctaat
     2881 agatagccga tataaggacc tgtggtcaag ccagaagagc ctagaccgct agcagccagt
     2941 aagcttggat catctggcag tacgccgaaa aatggggaaa aatctgacgt ataagcacgg
     3001 gtacctacac ggaactcgta tggccagttt tttatctgat ctggatcagt caagaactgt
     3061 tctgtactct tagtcaatcc cttataagct tcgctagtag gagccaaatc ccagccttga
     3121 tcgttttcat gggtagcacc taaaaggatc tttccatcca tgaaaggaat gaaatcagct
     3181 tctccgtcaa gcatagcaac aggccagtct tgactcatct tataagatgt ctgaaatgct
     3241 aagagctgcc ctttttgcgg acgtatatct gcagcatagc ctaattcttg taataaaggc
     3301 ttaagtgccg ggccaggtgt caaaattaca cgatcagctg tgtattctcc aaacggtccc
     3361 ttgatgagcc aagaatcgct cttcttatgc aaagtgactt ttgcctggat ctgctccatt
     3421 tgtttttgtg ctaaacgctg accaagatgt tctaaatatg cttttccatc cagacgacca
     3481 ccaccactga tacgcagtga aactgctggt ttcaataacg gaagtgcttg agatgtctgt
     3541 tcaggtgaaa gccattcgat gtttccaatt tctggcgctt cttcttttcg tttctcggca
     3601 agaacagcca attcagctaa atctttttca ggtcgaagca aaagtgtccc tgattgtgcg
     3661 taaatcgttt gatcaagttg gaaatctttt ataagtttag ggaaaaaagc agcgccgtct
     3721 ttagccagac gataccattg tttgtttctg cgtttcgaca accaagggga gataatccca
     3781 gcgctagctt tagttgcctg tcctgtccct tcatcaaata aagcaacatt gaattttgat
     3841 gtatctaagt aactggctag ggtcatacca atgatcccgc caccgataat ggcaattttt
     3901 tccataaaaa agcctccttt tacaaacaac cattttcaca caaaaaaacg gcagatgcaa
     3961 cacctgccgt atatggaatc tacgcttttt tagcaaagaa cagataatag gcgaatcgag
     4021 caaagcttac gagaagcaac agcaacaaga cgcctaatgc tgcttttgat cctaatgtag
     4081 tagacacttc atagttatgg gaagtatgat gctggacgta attgataatg accgctacta
     4141 atgaagtagc cacagcgcca gaaaactgct gcaaggtatt gaacattgcg ttcccgtctc
     4201 cttgtagttc ttcattcagt gcattcattc cggtggtcat catattgctg taagataatc
     4261 cgataccgat catataaaac acatgacaag caatcaatac acccactgaa gcgtgtccta
     4321 tgagtaggaa taatgctagc caaccaatca aagaaagaat cagcccagca aaaattggct
     4381 tcttcggacc atattgatcc agcattttcc cggaaaaagg agaaagcaaa gcaccaaccg
     4441 ctgcgccggg aagcatcgct aatccagcga caaaaggact acttccttga acgatctgaa
     4501 ggaaattcgg caagacaaaa gatacaccaa gtaataaaaa ctgataaact aagaagccaa
     4561 tcaaaaacaa gcggaatgtt ttatttgaaa gaatagacag tcggatcagt ggatgctcca
     4621 cttttttact tcgatagtaa aaaaggaacc agccaatacc gccgatgatt aaagcaatcc
     4681 agctggaaac tgttcccaac gtactcaaaa atgttagact tccactaaac aaaagacaaa
     4741 ttcctaaaac gcttatccaa tccaaataac cacttttttg tactgccatc tcgggaatag
     4801 caaaccagcc gataatcaaa gaaagaatca aaacgggcac aagaaacagg aagatatgat
     4861 tccatgtaaa atgtgaagtc ataatgcctc cataagtggg gccaattgct ggagcaatgg
     4921 ccgttgtcaa agtaccgaca cccatcatcg cgcctcgtcg attgaggggg gcaaatgtca
     4981 agataatatg gaacatcaac ggaagagcga tacctgtcgc tgcaccttgg agcaatcgac
     5041 caaacaatag gaccatgaag gtaggagaga agaaatcgat cattatacct gccaagaaaa
     5101 gcagatttga agctaaaaag agttttttga gactaaaatt gcgattcaaa taagtagaca
     5161 gcggaacaat gatcgaaatc atcaaaagat aaatcgttgt tacccattgg acttgtgcag
     5221 tcgttaaatc aaactgtctg ataagagttg ggaaagttac attcatggcc gtctcgatca
     5281 atacgcccgc aaaagacatc atccctgttg caattactgc aggtactaaa agcagctgtt
     5341 tttgttttgt catttacatc acctctaaaa aatttcgtaa aaaaagagag tgatttctat
     5401 tataaaaata gaaaccgcct ctctctcgac acgccgaaac gtgttatctg tacgatttat
     5461 ttacttcact tattctagca caaactgaaa aatagtgtca acacgattag ccaagtcact
     5521 caaaaagagg tatgataata aataactaaa tggaatttcg cggaggaaga aaaatggaaa
     5581 aaattgaatg gcgtaaagac aagagctata gtatgaaaaa aagcccgcaa atactagagg
     5641 ttcctaaaaa atcattcttt agcatcagag ggatcggcga cccgaatcag gaagacttta
     5701 aacgaagagt agcctgtcta tatgcagtca gctatgcaat acgcatgtcg cctaaaaaaa
     5761 attggctgat tcctgattat cagatgtata ctgtctatcc actggaagga caatggggac
     5821 tgcaagaaaa gtatttgcat gaaccagtga tgaaaaaaga acatttttct tatcaattga
     5881 tgatccgaca gcctgacttt gttacagaag cgatagcaca agaagccatt cagcgttcta
     5941 gcaaacttcc ggaagacttg aaagaacaag tagtattcaa aaagatggaa gaaggtcttt
     6001 gtgcccaaat cttacacgtc gggccctatg ccgaagaacc agcaacgttt gagaaattag
     6061 aagcattcat cgcaaaagaa ggctatgaaa gagcttctaa agaacacaaa gagatttaca
     6121 tcagcgatcc aagaaaatca gcacctgaaa aaatgaagac gattttgcgt gttaaaatag
     6181 aaaaaggtta gcaacctgaa tgaaaacaaa aaataaaagt aaaaaatacc aaaatatgaa
     6241 gaaatattac ttatcttttg ctataatcaa cattgagagg tgtctaatat gaaaaagaaa
     6301 cagcgacaag cactgatccg ccagatcatt acagaacaac ctattgggac acaggaagag
     6361 ctgcttgcgc ggctgcatga agcaggcgcc gatgtgactc aagcaacgat ttctcgagac
     6421 attcgagaaa tgaagttagt aaaaagtcaa aatgaaaaca aaatagtacg ctatacatta
     6481 tttaaccagc catctgtttc tttaaatgaa gaacgtttgc gtacagcaat tagacgcgag
     6541 gttttgcgta tccagtctgt tcagttcatg gttatcgtat tgactgaacg aaatggtgcc
     6601 gatgtggtaa cgaattggct agatgaagtg gcttaccctg aagttgtcgg aacgatggcc
     6661 ggtgtagata cattcattat tatttgccgc tcagaagaag aagcacagcg atttgcagaa
     6721 aagctggaaa aaatgaggga ataaatgagg aggactttta tatgaaagat atgaaagcgc
     6781 tgcaaaatgg atcggatatt cgaggaatcg cattaccagt tgaaggatat gaagtaaact
     6841 tgacgacaga catggtaaaa aagatcggct ggggactagc aaactggata aagaaaaaga
     6901 agaaatcaac cgatccaaat aaaaaatgga cgattgggat tggtcatgac agtaggataa
     6961 gcggaccagc actgaaagag gcgttgatcc aaagtctgaa agaacaaggg atacatgtca
     7021 tcgatttcga attagctact acgcctgcta tgtttatgtc cactcaattt gaacagttct
     7081 cgtgcgatgc tgcggttatg ctgactgcta gccatcttcc ctattattat aatggaataa
     7141 aattctttac ctcagaagga ggagcagaaa aagaagatat tgcttttatc ctatcacagc
     7201 agcaaaaaat aaaaagcgaa caaactggaa gcgtagaaaa agcagacctt ttgacacctt
     7261 atgcgcaaga tttagtcaca aaaattaaaa aggggattgg aacactagaa agcaaaccac
     7321 taaatggttg gaaaatcatt gttgatgccg gaaatggtgc tggtggattc tttgctgaaa
     7381 aagtattgaa tagcttaggt gcggattcaa caggttccca atttttggaa ccagatggcc
     7441 gatttcctaa tcatatccca aatcctgata ataaagaagc gatggaaagt atcaagcagg
     7501 ctgttttagc aaatgaagca gatcttggga tcattttcga tacagatgtg gatcgttctg
     7561 cagttgtcag tgcttctgga acagtgatca atcgaaataa tttgatcgca gtattaagta
     7621 caatcgtatt aaaagagcat ccaggaacaa gaatcgtgac gaattcccct acttcttctc
     7681 atctacaaag attcattgaa gaaaaaggag gcatccaagt tcgttatatt tccggctatc
     7741 gaaatgtaat caataaagca ttagaactta accgtcttgg gaaagataca cagctagcca
     7801 tcgaaacaag cggacacgct gcattcaagg aaaactattt tctggatgat ggtgcatatg
     7861 tcatcgcaaa gatcctgatg cttttgccag aattaaaaag aaaaggtatc acattagatg
     7921 aacaaatcaa agagttgaaa cagcccgtag aagtacaaga aatccgatta aaattgttca
     7981 gcgatgaacc acgcgaacaa ggaaacaaga tattagaaga aatgaagcga ctgttggaag
     8041 aagaaaaagg gatgaacttc gacccggaaa atgaagaagg tattcgatat gatctagaag
     8101 agccatacgg aaacggctgg tttttgatcc gtttaagctt acatgagccg ttactagtat
     8161 tacagataga aaatgatgaa ttcggaaaga acaagttggc gttagtaaaa ttagcaaaat
     8221 tattcggaca tttttctgat cttgaatcat taacagcaga ataaaccagt ctttttgaaa
     8281 ggctagttgc ttggacggca aaaaaagtgg tatggttaaa tcgagtgctg gtagggag
//
LOCUS       DAXWLB010000122         8265 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXWLB010000122 DAXWLB010000000
VERSION     DAXWLB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.122"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(78..2357)
                     /gene="helD"
                     /locus_tag="ACGE4H_002117"
     CDS             complement(78..2357)
                     /gene="helD"
                     /locus_tag="ACGE4H_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFF8365513.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKNLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            2635..4458
                     /locus_tag="ACGE4H_002118"
     CDS             2635..4458
                     /locus_tag="ACGE4H_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFF8365514.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            4505..4684
                     /locus_tag="ACGE4H_002119"
     CDS             4505..4684
                     /locus_tag="ACGE4H_002119"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFF8365515.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5021..6961
                     /gene="thrS"
                     /locus_tag="ACGE4H_002120"
     CDS             5021..6961
                     /gene="thrS"
                     /locus_tag="ACGE4H_002120"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFF8365516.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7185..8033
                     /locus_tag="ACGE4H_002121"
     CDS             7185..8033
                     /locus_tag="ACGE4H_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8365517.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
BASE COUNT         2605 a         1481 c         1738 g         2441 t
ORIGIN      
        1 tgaattactt tttctgcaat tattattgta aataaaaaga caactgctaa cattaatgat
       61 agcagttgtc tttttactta cagtactttt ttctcataag atgaaatatc catattgttt
      121 aacaaaggac tttcattatt ggaggtaaaa agggtcaatt catgcatcgc acgtgtacac
      181 atggtgtaaa ggatcaattt gtcttgctga gaagaaaaat gttctgcatc tacatcccac
      241 aagaaaactc ggtcaaactc cagacctttt gctaaaaagg ctggaagtaa aataatggaa
      301 cgcttcataa agtcactctc atttaaaatc agctggactt gttgtttcaa ctcgtctggc
      361 agttgagcat aaagagcttc gcattcttct tgtgttttag ctatgattgc tgtacgccaa
      421 taccgggctt ttccctgagt cgttcgaatg gtatctgcta accaattcaa tttgatatca
      481 tctgttgttc cttggatgag caaaggaagg tctccttttc ttgctgttgt ttctacttga
      541 tgttctttca caataaaatg attagcaaaa tcagtgattt cttttgtgga gcgatagctt
      601 gtcgttaatt ggaaacgagt gacttcacgg tccacgaaaa ggtcttccaa tgatccaacg
      661 atcgtttcat ttccaaagac attttgattt agatctccac agaaagtcaa atttgctttt
      721 gggaacaagg cttgcagcaa tgccacttgt gatggtgcga aatcctgcat ttcatcaata
      781 aagatatagc gagctttgtg tgtaaccgtc actggatatg ttcggcgcat cagtaaaaag
      841 aatagtacag catcctcttg acgcaattct ctttgacgca agtgtgtacg aacagttgtc
      901 aaatgttcct gccacctggt ctcgctgatt ttatgtcgtg ttagaagaga aagtggcaca
      961 gtttgtagaa agtggagata ttgtttcgtt atattgacaa attgataatt catgatccct
     1021 cgatggattc gacggaatct tttacgtacg atttggttgg ccaggttttt acgtaatgaa
     1081 cgctcctttt gttctgtata ttcaagattt ggatctgttg cgtatagttc ttggagctgt
     1141 tcttccgcca gatctttgac ccattgctga cgtgtttcgt ctttttgcag accgcctaat
     1201 tttttcagga gcttcgtttg taatagagaa agtctatgat gtaaaggtaa ttgttcattt
     1261 gtttctttat accattggcg aatcgattcc tttgatagga tcgtacggcc atttattttc
     1321 atatcacgga acaagggacc gtagtttgtg atgccttgga tatagcgatc aatctgatcg
     1381 actaaggtca atccgctttt catcacttgt atcggatctt tttcccctag caaaaaacca
     1441 ctttcttgtt gctgttcatc tagtaaagaa aactctggca gcagctgcac caaataattt
     1501 ttgaatgtct gtgtaggcac gccgctttca cctaatgaag gcaatacagt cgagatgtaa
     1561 tcagaaaaca aatggttggg tgaaaataga agaacgtttt cagcctcgag ccacttgcga
     1621 ttatgataca acagaaaagc aatacgttga agcaaagcgg atgtctttcc acttccggcg
     1681 attccttcaa tcagcattac tttacttgtt gtgtcccgaa tgattgcatt ttgtgctttt
     1741 tgtatcgtag agacgatgtt tttcatatgg ttgcttgatg attcatccaa tatctctagc
     1801 aaaaagtcat cattgattac ttccgacgta tctaccatag ataaaatacg tccttcttgg
     1861 attttaaatt gtcgtttcaa ctgcagatcg acatctattc tatctgtttc agtttcataa
     1921 aatgcgggac caatctcacc ttcataataa agattggcaa ttggtgcgcg ccagtcaata
     1981 acgattgttt cttctgccag atctcttaaa gaagcgatac ctagatataa tgtttctgtt
     2041 tcacggtctt ctttaaagtc gatacgggca aaataaggac tatctttcat gacagacaac
     2101 gtttgcaggc gtttttgctg tgcttcaaga gtatggtatt tcaaaagcaa ttcctgctca
     2161 tgttggcgat attcaacagc agattcataa aaagcttcat tggaaccagc tcgaatcttg
     2221 tgattagctg tgtcttttgt cgaatgattc atttcctgag tcaattcttg ttgttttgta
     2281 tccagcatat tttgttcgat ctggattagt tcgatcgttt catccacgtg cgtttgttct
     2341 agttgtttct cattcatcgc atgtttgctc cttccgccgt aaataacgaa ttaaaagtat
     2401 agcatagttt ttcgaaaaaa ggtacttttt aactgatgaa tcgaaaaagt aatccttgct
     2461 gaatattttt gtttgtagaa ctgatgaatt ttggaaacgc tgatagtgat agttgttttc
     2521 aattaaggcc tattgtttta aaaagtgcta gtcaaggact atgcttaaga tgaaagaggc
     2581 agctggaaat ttattactaa cctctctaag tcacataaaa gaggagaaaa aacaatgaca
     2641 catttcaaaa aatttttact tactttgctt attggtggga tagctttatt aagtgaattt
     2701 gtttttcacc agccggccct tgcatttgcg attgtcgcat ttactggagg gattctcgcc
     2761 ttcttgatgt ttttggaaat gatcaagacg ttacgttcag gaagatatgg cgtagatgta
     2821 ttagcgatta ccgcaatcct tgcaacatta gccgtcaaag aatactgggc tagtttgatg
     2881 atattgatca tgcttacggg cggagatagt ctagaggatt acgcgaataa acgagcgggt
     2941 aaagaactgc agtctttgtt agataacacc ccaagaattg cccatcgatt caaagaagga
     3001 aaattgcagg atatagtaat cgatgaaata aatattgatg accagttact agtcaagcct
     3061 ggcgaacttg tacccgcaga tggtcgagtg accaaaggag aatcttcggt cgatgaatct
     3121 tctttgacag gtgagtcccg cccggaaacc aagaaacctg gagatcagat tatgtcaggg
     3181 tctttaaatg gagacggatc gatccaaatg atcgctgaaa aacgtgccgc agacagccag
     3241 tatcaaatga ttattaaact agtcgaagaa tcaaaagaaa agcctgcccg atttgtccgt
     3301 ttagctgatc gttatgcagt ccctttcacg ttgattgctt atttgatcgg tggtatttct
     3361 tggtggttct caaaagaccc tttacgattt gctgaggtac tggtagtggc atcaccttgt
     3421 ccactgattc tagctgcgcc cattgcttta gttgcaggaa tgagccgttc aagtaaaaac
     3481 gggatcgttg taaaaactgg aacaacgatt gaaaagctcg cacgtacaaa aacgattgct
     3541 tttgataaaa caggaacgct taccaaaggg atattggaag tagaggcgat tcagccagtc
     3601 aatggaataa aaaaagaaga gctacttctg tatgcagcta gtgcagaaca ggaatctagc
     3661 catatcttag ctcgttcgtt agtgaacaac gtgaaaaaca gccaactgta tcctgttagt
     3721 caactgaaag aagtgaccgg tcaaggaatc caagcagtag tcaataatca tgaagtaaag
     3781 attggacgtg cctcttttgt ttcagctgaa gaaatagaaa cgtccaatac agcaatctat
     3841 gtaacaattg atcgatcttt tgcaggaacg atcctatttt cggatcagat tcgcccagaa
     3901 gcagcaaaga caatcaagga gctaagaaaa cttgatgtga aaaatttgat gatgatcacc
     3961 ggggatggat ctgccattgc tcaaacgatt gcagaaaaag tccaattaga ttctgtacat
     4021 gcccgtagct tgcctcaaga taaactgacc attttagaaa gtatccctaa agatcaaaga
     4081 cctgttgcga tggtaggaga cggcgtgaat gacgcaccag cacttgctgc tgcagatatc
     4141 ggtattgcaa tgggggctca tggttctaca gctgctagtg aaagtgcaga tgcggtcatc
     4201 ttgaaagatg atctcttgcg tgtagcaaaa gcagtagtga tttcaagaga tacgatgaga
     4261 atagcgaaac agtctgtact gattggcatc tttatttgta tttttttgat gttaattgca
     4321 agtacgggac taatccccgc attattcgga gctgttttgc aagaagtcgt ggatacggtc
     4381 tctattttga gtgccttgag agcaaaaaaa gatccagcca tttttcctga ttcagtaccc
     4441 aagacaaaca tagtatgata gaatcgagtt gaaaaaagaa cgatcagaaa gaggagagag
     4501 cattatgcca ttcgtacatg tcgaattagt agaaggtcgc agtccggaac aattagaaaa
     4561 tatgatgaaa gatatcacgg aagctgttca taaaaacacg caggcaccaa aagaacacat
     4621 ccatgtcatc atcaatgaga tgaaaaaagg aacatacggt gtaaatgggg aatggaaaaa
     4681 ataagtttag tttcccactt gatttttcta tttattagtg gttaaataaa cacataattg
     4741 attgatgaga aagccaactg aatttgagag tcttcgataa gagaggaaaa gctaggctga
     4801 aacttttcca ggagaaaacc aaaggatgac cactttcgaa acccttgttg aagcaagggc
     4861 ggtcggaccg ttacaacgct tgaggaacgc atttgcgttt gaacattagg tggaaccacg
     4921 aacttttcgt cctagtatct cttttcaaga gatgctagga tttttttgtt tttcagcttg
     4981 atgatctgat caatcaaaac atttcaagga ggaaactatt atgataaaaa ttacttttcc
     5041 agatggtgca gtcaaagaat ttgagtctgg aacaacaaca ttggcaattg ctgaaagcat
     5101 ttcaaaaagc ttagcaaaaa aagctttagc tggtaaagtg aacggaaaac tcgttgattt
     5161 gactcgtcca atcgaggaag atgcaagtat tgagatcatc acaccagacc atgaagatgc
     5221 acttggctta gtccgtcatt cagcagctca tttgatggcc caagccatgc gtcgcctcta
     5281 tccaaatatc cactttggcg ttggaccagc tatcgattca gggttttact acgatacaga
     5341 taatggacaa aaccaagtaa ctgcagaaga tttgccggca atcgaagcag aaatgatgaa
     5401 aatcgtcaaa gaaaatctgc cgatcgaacg ccgtgttctt tcaaaacagg aagcattaga
     5461 aatttttgca agtgatccgt acaaagtaga actgattagt gaattgccag aagaagaagt
     5521 gattactgct tatcaacaag gcgaattcat cgatttgtgt cgtggaccac atgttccatc
     5581 cactggccgt atacaagtat ttaaattatt atcagtggca ggtgcttatt ggcgtggaaa
     5641 ctcaaacaat caaatgatgc aacgtgtata cgggacagcc ttttttgaca agaaagcttt
     5701 gaaagaatat atccgtctcc gtgaagaagc aaaagaaaga gaccatcgta aactaggaaa
     5761 agaacttgac ttgttcatgg tttccccaga agtaggttct ggtttgccat tctggcttcc
     5821 aaaaggggca acaatccgac gcacgatcga acgttatatc gtagacaaag aagttagtct
     5881 aggttaccaa catgtatata caccaataat gggagatgtt gagttgtaca aaacatctgg
     5941 acactgggat cattatcaag aagacatgtt cccgccaatg gatatgggag atggcgaaat
     6001 gcttgttctt cgcccaatga actgccctca tcacatgatg gtctacaaaa acacgatcca
     6061 ttcttatcgt gaattaccaa ttcgtatcgc tgaattagga atgatgcatc gctatgaaaa
     6121 atcaggtgct ttgtcaggtt tacaacgtgt acgagaaatg acgttgaatg atggtcatac
     6181 atttgttcgt cctgatcaaa tcaaagatga atttaaacgt acgcttgaat tgatggtggc
     6241 agtatatgct gacttcaata ttacagatta tcgtttccgc ttgagctatc gcgatccaaa
     6301 caatacagat aaatattttg atgatgacgc aatgtgggaa aaagcccaag caatgctgaa
     6361 agccgctatg gatgaattag aactagatta ctttgaagca gaaggcgagg cagcctttta
     6421 cggaccaaaa ctagatgttc aagtcaaaac agctcttggt acagaagaaa cattatcaac
     6481 tatacaatta gacttcttat tgccagagcg ctttgatttg acatatgttg gagaagacgg
     6541 ggaaaatacg catcgtccag tagttatcca ccgcggaatt gtttctacaa tggaacgttt
     6601 tgtcgcttat ttaacagaag tatacaaagg cgcttttcca acatggttgg cacctataca
     6661 agcaacaatc atccctgttt ctgttgatgc acatggcgat tatgcttatg aaatcaaaga
     6721 acgtttgcaa atgaaggggt tgcgtgtaga agttgatgat cgtaatgaaa aaatgggcta
     6781 taagatacgt gcctctcaaa cacaaaagat cccttaccaa ttagttgttg gagacaagga
     6841 attggaagat gcaacagtga acgtacgtcg ttatggaagt aaagaaacag ctgtagaaga
     6901 tttgaatatc tttattgatg ctatggaagc agaagtcaaa aattacagca gagaaaacta
     6961 ataggtaaat ttcctgtaaa aaagtagtag atagaaagca atccgaacaa tagataacag
     7021 gtattcctca aagacacaga tgctcaatag aatctgtggg attgatggga taagatattc
     7081 tgttgggcgg attgttttta tgttttttat ccagtatacg accgtgaata agggtctgat
     7141 atggtatact tacatagact atcattaaaa gggtggagga acttatgagc gtaattagtc
     7201 gtattgaaag tatgatggaa gattattctt cagcagaaaa aaagttggcc aactacatta
     7261 tcggtaatat tgagaaggtc cctacaatga cagctaatga attagcagaa gcttccgggt
     7321 tgagtgcgcc gacagttgta cgtttttcca aaaaaatcgg ttttcaaagt ctgacggact
     7381 tcaaaatcac gatttcaaca gaactacaga ctggaatcga tgaaggtttt agtgatatag
     7441 aagcaaatga gtccttttac tctatcaaaa acaaacttgg taacaatgca caagtagcga
     7501 taaaagaaac agtagatatt ctagaagaag aaacgatcca aaaagtcgta gagtgtttgg
     7561 aatcggcaga gactgttttt ttatatgggg ttggcgcctc ttctttagta gtagaagata
     7621 ttctgcaaaa atggtctcgt gtcggtaagc caatcatttt tgaaaaagat atccacgtct
     7681 tgctcccgca acttgtcagc aatgagaaaa agaaagtgct ttggcttgtt tcaaactcag
     7741 gtagaagtgc agatgtagtc gcattagccg agctggcgaa aagcatgaat atcgaaatca
     7801 ttgcgttgac gcaatttgga aataacccgc ttagcaagat agccgatgta ctcgtacaaa
     7861 catctagacc aaaagaaatc acgaatcgaa gtgcggcaac taattcactt ttggcccaat
     7921 ttgctacgat cgatatcatt ttttatttct atatggctaa aaacgaaaag cttagcgaaa
     7981 aagtgaaaaa aacacgagaa gcaatccaag aatattttat ggattcgaaa taaaaacctt
     8041 actatttttt atataagttt tttagaataa caaaacgtag ttttccacaa ataaatgatc
     8101 catcaaagtg tttcacgact taagaaaaca taattttcaa aaataagttt attttttaat
     8161 gaaaaaaata gttggatatg taactttaag tgattgctgt gaaacaaaaa gttgatttta
     8221 tcttgaaatt aggtaaagaa tttcttgctc cgcccatttt ttctt
//
LOCUS       DAXWLB010000123         8229 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXWLB010000123 DAXWLB010000000
VERSION     DAXWLB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.123"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACGE4H_002122"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACGE4H_002122"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFF8365518.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE4H_002123"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACGE4H_002123"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFF8365519.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACGE4H_002124"
     CDS             2591..3376
                     /locus_tag="ACGE4H_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8365520.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACGE4H_002125"
     CDS             3389..4240
                     /locus_tag="ACGE4H_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFF8365521.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACGE4H_002126"
     CDS             complement(4364..4816)
                     /locus_tag="ACGE4H_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365522.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACGE4H_002127"
     CDS             complement(5281..5649)
                     /locus_tag="ACGE4H_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFF8365523.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACGE4H_002128"
     CDS             5799..6281
                     /locus_tag="ACGE4H_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFF8365524.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACGE4H_002129"
     CDS             6318..7328
                     /locus_tag="ACGE4H_002129"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFF8365525.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACGE4H_002130"
     CDS             complement(7443..8156)
                     /locus_tag="ACGE4H_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365526.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2441 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtct
//
LOCUS       DAXWLB010000124         8221 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXWLB010000124 DAXWLB010000000
VERSION     DAXWLB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.124"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(450..1232)
                     /locus_tag="ACGE4H_002131"
                     /pseudo
     CDS             complement(450..1232)
                     /locus_tag="ACGE4H_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1297..2133)
                     /locus_tag="ACGE4H_002132"
     CDS             complement(1297..2133)
                     /locus_tag="ACGE4H_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8365527.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2151..3560)
                     /locus_tag="ACGE4H_002133"
     CDS             complement(2151..3560)
                     /locus_tag="ACGE4H_002133"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8365528.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3574..5397)
                     /locus_tag="ACGE4H_002134"
     CDS             complement(3574..5397)
                     /locus_tag="ACGE4H_002134"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFF8365529.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6240..6566)
                     /locus_tag="ACGE4H_002135"
     CDS             complement(6240..6566)
                     /locus_tag="ACGE4H_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365530.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6612..6974)
                     /locus_tag="ACGE4H_002136"
     CDS             complement(6612..6974)
                     /locus_tag="ACGE4H_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFF8365531.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7011..7226)
                     /locus_tag="ACGE4H_002137"
     CDS             complement(7011..7226)
                     /locus_tag="ACGE4H_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8365532.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7353..7688
                     /locus_tag="ACGE4H_002138"
     CDS             7353..7688
                     /locus_tag="ACGE4H_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8365533.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7764..>8221)
                     /locus_tag="ACGE4H_002139"
     CDS             complement(7764..>8221)
                     /locus_tag="ACGE4H_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365534.1"
                     /translation="EKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTE
                     KELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLI
                     YLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2538 a         1738 c         1269 g         2676 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaagaaa gaaacttatt cttgctaata aactacgcga
       61 aaggagtcta acgttgtttg atataactta gactgatcgt taacttattt tttatatttc
      121 tacatagtta tctttagaac catttcagat acatatgaaa caattacata tgattttttc
      181 ataaaaaaac agtactgtct agtagtataa aatatacaaa tttcttaaaa attttttcac
      241 taaaatcact acctgaaaca gtctaaaaca cttatatatc aacgtttaat gaaactatct
      301 ccgtttcatt tcagatacaa ataaatatag tcgggctcca aatccgaaaa aaaatacccc
      361 cagtagctta tcctaagcta ctggagatgt ataagtacgg tcatataaca acggggtagg
      421 gtaaataaat gaaaaagatg gaagaatcat tattcaataa cttttttaaa tacttcgttg
      481 taatcagtta cctgtccgtt cttaaatacc acagaataat ctttttaccc attttcaagt
      541 aaccgaagtg gaatgtcttt accaacttgc tctgtcactg cattactgat acctgttaga
      601 ttagctaata aatctgataa agattgagca tctgtagaaa tctctgtatc agtatttatc
      661 gtgattcctt tatcttcatt tacaagcttc caagtaggtg gtatagctgt gtatgtttcg
      721 atgaatgaat caattctttc atctatcgtc ttcccagtat ctacttctgt ctcattactc
      781 ttagatgaat cgattctttc atccatcgtc tgctccgtaa ctgcttctgt ctcattattc
      841 tcaccggaaa ctctcttcac acccaaactt atatattgtc catctttagt gtgcatagtt
      901 aaaattccca cgcttttact aataaattca ctataaccag ttctttcaat agagacttca
      961 atgtatttca cactatcata ctcaaaagaa aaatagacat gtaaaagcat tttgttgttt
     1021 acatctacat aataatcctt tgactctact tgagaccttc ctatacggaa tacagcttta
     1081 ctgtctgata tctctatacc agcatcctta ttattaatct gccaatctcc aagtagctct
     1141 tctggcaagc tagtagtttc ttgtgaacac tttaccactt cactggtaga ggtcgaaaca
     1201 ttcacctccg cactgttaga agaattttcc aaggaatcag gagccttagc acaaccaaca
     1261 agtctttata tattgtgcag ccgatatttt cttcattcag tacacccttg aaatatgcag
     1321 tgttagataa agcagttcgt cctgatttgc tgaggcaccc atctcttctt gaacgtactc
     1381 cttgattttc aaggccgccc gatactcttt cggaaaacgc tgcttcactg aagccagcaa
     1441 aaaatcatcc ttctccggca actcttcctt cttaatcatt cgggcaataa aaaactgtaa
     1501 atgcgtcaca aatcttaagt aaagcatcga atctttatca accttttttt cgggaaacag
     1561 atatcctagc atagccaaga tatcctttag tgctcttacc gagagcatgg tttcttccat
     1621 atttgcttgt tcactttggg catttacaat gtgaaacgca atgtttccag cctcctcttc
     1681 tggcaactga acattcattt ttttgttgat caattggata gcatagcagc cgatctcaaa
     1741 ttcttttggg taatactttt gaatttcaat cagcaggcga ttttgaaaaa caatgccttt
     1801 ttgactgcgt tcaatggcat gcttcaagtg atcaaccaag gtaaaaaata gacttttatt
     1861 cagcttaaag tcaaacttgg cgttggcata atcgataatt cgcttgacgc aaataattag
     1921 atcatccgga gcagcctcaa acagtttaat gtaatccttt tttagagcat cagaaagtga
     1981 atctaattta aatacttttt ggattttgtc agccggaacc aaatcgcctt tctttagttg
     2041 aaatccaatc cctttcccca tcaaaattac ttctttgcct tgcttatcta agcaaagaat
     2101 taaactgttg tttaacgttt taattactct cattagattc accgttctct ctaagctaag
     2161 ccatttcttg cgattacttt ttgataccaa aagaagcttt ctttttttat tcgttttaat
     2221 tctttaagat tctcatcggt tcggtcaaca tatataaaac cataacgctt attgaagcct
     2281 tgatgggtac taacaacatc aatcgctgac caaggtaaat aaccaaaaac atctacacca
     2341 tctgttaaag ctgcttgaat agccagaata tgtttggcta gataatcaat tcgatcctgg
     2401 tcattaattc gtctatcttc ctcaactatg tcgggtgccc catagccatt ttcagtaatt
     2461 aaaataggta aatcatagcg atcgtacaat ttcctcaatg ttaatcgtag tccagtaggg
     2521 tcaactaccc agccatatgg cgttttatct acataaggat tttcagctgc ccggtagaca
     2581 ccttcttctc ccaacatgat ctgttgatcc cccgctctag cggaaacatc tgatccgtca
     2641 tttttgctgg cagcgatggt ggcagttgaa taataattaa tcgcaataaa atcgggttta
     2701 ccggaggata aaattgcctg atcctctgcc tcaattttag gatataatcc acgatcattc
     2761 aaatagtttt caaacaaata attgtatttt ccccgaactg ctacatctaa gaaagaccag
     2821 cctctcaatg tttcccaatt atgggctgca attgcatctt ctggactgga ggtagcttga
     2881 tacatagccg ttaaattgat tgctggaccg atttttcctt taagattcaa ctgatgatac
     2941 aagtgaatta ctctagcttg agctaataaa acatggtgat taatttgaaa gagttctttt
     3001 tttgaaggca gctctccact tggtggcaaa ccaatcgctc caggatgaag gatcatcgtg
     3061 ttttgctcat taattgtcag ccaatagttc accttgtcac catactcagt aagcaatacc
     3121 tgtacatagt ccacaaaata atcgatagtt gctcgattta accaaccgcc ctgctcctct
     3181 agaaaataag gtaaatcgaa atggtacatg gtgacaatcg gttcgattcc ctgattcagg
     3241 cattcatcaa ttacttgatg ataaaaagaa attccctttt gattggtttt attattttca
     3301 atatccataa ttcgcgtcca agcgatggaa aagcgaaaag cttttaagcc taattcttta
     3361 aacagctgta tatcttcttt atagtgatgg taaaaatccg aggcaacttc aaaattagct
     3421 accccttctg tatgctgata cttgtcaata atagacggtt gtttcccatc ctcctgatag
     3481 gcaccctctg cctggtaggc actaatggag gcaccccata aaaacttctc tggaaatgct
     3541 gaaagctgtt tgtattgcac aaaaatcctc tccttaacta atttgcatca atggttcgtg
     3601 ttcctttaag cttccgcttg cttcactaat tcctgcgtaa ttttcactgt tggtcactac
     3661 cacaatcact gtatcatcgt agccagcgtt tctaatggca gcgaaatcgg cgttgattaa
     3721 taattgtcct gctgacacct gatctcctgc ttttacaaat acttcaaagg gttccccttt
     3781 taaattcacg gtgtcgatcc ccacatggat caataattca ataccttctt tagaacgtag
     3841 accaatcgca tgtttagatt ctaaaatagt aatgacttct ccctcaacag gtgaataaat
     3901 gctgttttct tctggatgaa tggctacgcc ctttcccaat agtcccttag aaaagacagc
     3961 atcgttcact tcagaaatag gacacacggt tccttttacc ggattttcaa caatgactgc
     4021 ttcggctatt gccgtttgct ctgattcatc agaagtatcg tcaaatccaa ttagataagc
     4081 aatgataaag gttgcaacaa aggcgattac acacgtaata attgcgtgaa caatattcat
     4141 tggattttct ccaataaagg ctggtaaggc tgctaagcca ggggaaacaa aggcatatct
     4201 gactaaaccg ctcaagccag catacaaacc agcaattgca ccaccaatca tggaagcaac
     4261 taatggtttt ttcagcttca aggttactcc atagagtgaa ggctcagtaa ttcccattaa
     4321 agcagtgatc ccagtggaca cagctagctg cttcaactct ttgtttttag ttttcgcggc
     4381 tacagcaaag gtagcacctg attgagcaat attagaagcc agcattcctg gcccattgat
     4441 catttcagaa cccgtactgc tcaattgcat agtagcgatt ggcgtcattg cccaagctgt
     4501 acccgtaacc accaaaaatg gctgcaaacc acccatcaaa aatggaatta accaagctgc
     4561 tttgccgtta atccattcag cccccattgc tataagatcg ttcagcaacg ttccaaacgg
     4621 tccaatgaca accaacgcta aaggccccat aattaaaaat accagcatcg gcttagtaaa
     4681 gaatttaata aacgaaggcg aaattcgatc cgcaaagggc tctacatagg acatgatcca
     4741 aacagtaaag ataatcggta aaactgatcc cgcataatct gcagctaata ccggaattcc
     4801 tagaaaagaa acggcatcgc cagtagaaaa aagctgtgaa atatttggat gaagcaaaat
     4861 gccagcaaca gtaatcgcca aaattgggtt gacatcaaac tttatggctg ccccataggc
     4921 taatagtact ggcataaaat aaaaggctgc atcagcaata gtattcaaaa tgatgtaagt
     4981 ttgactactg tctgatagta caccagtcaa agtcaagacg gccaaaatgg cttttaccat
     5041 cccggcacct gttatagctg gaatcatcgg tgtaaaggtg gtagaaatga cactaatcaa
     5101 tcttgaaata atactttctt gattgtctga agaggggccc tttttaattg aagttcccac
     5161 ttccttttga atttccttga aggctgtttg gacttcattg ccaatcacca cttggtattg
     5221 tccaccgcta tccaccacac taattacacc aagtgttttt tctaaggctt tcgtatcggc
     5281 tttcctttta tctttgagtt caaaacgcag tctggtggca caatgggtta agctgaccac
     5341 attttctttt cccccaacct gttgcaatac atttcttcct acttctctgt aatccattat
     5401 tctctctcct taaagcagta aaaaagacca agtcaaacca cgccccaatc aacattgaag
     5461 tgttagtttg tcttggtctt gcctgcataa tcagtaacat gccaataacg ttattttgct
     5521 gtaatccaat aatagcacat tatgatagcg caatcaacag ggaatttaat tttttatctt
     5581 ttcgtttgac cgtatagggt aatataacag ttagatcaca tcaactaggg tatatctgcg
     5641 tatacttact gctaaaataa gtatatctaa tattaactat accagtaaaa ccattcctac
     5701 ataaccagcg ggaaattccc aaaatcgtga aagcatcaaa aatgcatata acggatcaca
     5761 caacttcaac aattctgctc ttgggaaaaa ttgatgaatt tggtgagtaa aggatggaat
     5821 aaacagcatt ccaacaatat aggcaacaat agtgatactt gtttttttgg taaaaatcag
     5881 taagcaatag ccaatttcaa tcattccgag atataaaggt atcaatagaa tgatttgggc
     5941 tagtattctt aaaaattaac tagcaaatgt ttaaactgat ttatttctaa tctacatgta
     6001 gtaagtattt caattccacc aattatcaat aaaaatacca aacatatcca taataatttt
     6061 ttctttttca tttcttttat attgttataa cagtgaataa cataaaaatt cccgacaatc
     6121 attgtaatat ttttttgtca cctgtattag tatttaattt ataggaaact ataaaaactc
     6181 cctagtgaat ttacaccttt gggagttcct gtttggacgg tatgcataat acatttaaat
     6241 caatttaaca ttaacacctc agcatatggt atctccacat atatacggtc tttctttgga
     6301 tagtactcaa tatttatgtt gatagcactt ccattgtcta atatagcttt agcccggtat
     6361 gtgttatctc cattttcatg agtaatcgtc acatcatcac atgatcgatt taagttgtac
     6421 tgttcttgta aaatctgtgt taccaatggt tttgatgact tctctaatcc agatgtacct
     6481 gtcatttttg aaaaaataat acctataata gccaagacaa caatacctat aatgacaaac
     6541 ttcttgttca tatttacttt attcaacttt ttatctccta tctccaaaat aatttgttct
     6601 tatccctctt atcagtacat tgacatccct aaaagtacac caaagattgc tccagctacc
     6661 cctgctatca tcaaaatcgt acccattttc tcatcttttt ctcgatataa gtagcccata
     6721 ataacagcaa cagcaccaaa tacaacagga ataaatgcta acgatactac catagaaatc
     6781 caaccaataa tcaaatacag cttccctgtc tcttgtccat gtacgatttg ttccttcatt
     6841 tcattaattg tctgctcagt acttttcttt actgtgtctg ttccggttgt ctcatctgtt
     6901 tcttgtagtg ctccacaaaa ctggcaatac ttactttcat ctgttatttc tttgccacat
     6961 tttctacaat acatgtcttc tcctcctaaa atcattcaat tgcttattag ttaactgttt
     7021 actttattaa atacatcaga aaacagctca ttcattggta cacttaatat ctcagaaagc
     7081 atcttcattt gatagggttt aggaatcgtt ctccctgttt gccacgaact tacacattgc
     7141 tgtgtaacat tcattttttt tgctaactcc gtttgagaaa gtcctttttc ttttctgtat
     7201 cggctgagat tatttacttt tttcatgatt ctcccctcct gttttattat atacaacaat
     7261 atgatgtatt tcaactcata aaatataaaa aacttcaaat accattacaa atacaacaac
     7321 ttgttgtact atactgagat gggggtgtat taatgaaaca gaaacaatat gcaaaggtca
     7381 ttagagaaaa gcgaaaagct ctcgggtgga cacaaaaaga attagctgaa aaaatcttct
     7441 ctacacaaca ggcagtagca agatgggaaa attctgtaac agagcctaac ctcgatagct
     7501 taacagctct ttctagagcc ctaggaaccc ctgtaagcca ttttctagac aatgttgtcg
     7561 tagattatga ggaagagttt ttggctctct atcgttctct tagcactgaa gatgcagttc
     7621 gaacaattga ctatatgaag ctattaaaaa ggcaagaaaa tgaacgcaat cagctattga
     7681 aagattgaaa aataaataac ccaccgaact ttaacggtgg gtgtattgag atgaaataag
     7741 attaagagct atggataacc tgtctagttt aatacaattg ggtcttttct cttccttact
     7801 ctccgtccat catctcttgt tacacttgta aattgataca gaggaactat cccagctcct
     7861 gataatatca attcttttat tgtttcatct atccctgccc ctttcgctaa atatattaaa
     7921 aaactactac ttgctttcac attgtatagt ttcttaatat ccttagaagc tcgttcaaga
     7981 gtgaatcctt gtttaacaaa gtagcctctg ttttttatta aagcattttt cacgctgtta
     8041 tccccaacat tacgtaggcg ttcacaaaca gtgttaataa gttctttctc tgtcattaga
     8101 tatgtccctt gccacgataa gccctgtaaa acttcatgtg cttcatctaa aatcaattgt
     8161 ttttccttgc tgagttctga tcgggaattt tgaggaaata ctcttttagc ctctttttcg
     8221 t
//
LOCUS       DAXWLB010000125         8129 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXWLB010000125 DAXWLB010000000
VERSION     DAXWLB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.125"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(342..1241)
                     /locus_tag="ACGE4H_002140"
     CDS             complement(342..1241)
                     /locus_tag="ACGE4H_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFF8365535.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
     gene            complement(1262..1535)
                     /locus_tag="ACGE4H_002141"
                     /pseudo
     CDS             complement(1262..1535)
                     /locus_tag="ACGE4H_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            complement(1558..2964)
                     /gene="lacG"
                     /locus_tag="ACGE4H_002142"
     CDS             complement(1558..2964)
                     /gene="lacG"
                     /locus_tag="ACGE4H_002142"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFF8365536.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            complement(3106..4812)
                     /locus_tag="ACGE4H_002143"
     CDS             complement(3106..4812)
                     /locus_tag="ACGE4H_002143"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFF8365537.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            complement(4812..5129)
                     /locus_tag="ACGE4H_002144"
     CDS             complement(4812..5129)
                     /locus_tag="ACGE4H_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFF8365538.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            complement(5157..6137)
                     /gene="lacD"
                     /locus_tag="ACGE4H_002145"
     CDS             complement(5157..6137)
                     /gene="lacD"
                     /locus_tag="ACGE4H_002145"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8365539.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            complement(6140..7072)
                     /gene="lacC"
                     /locus_tag="ACGE4H_002146"
     CDS             complement(6140..7072)
                     /gene="lacC"
                     /locus_tag="ACGE4H_002146"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFF8365540.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            complement(7083..7598)
                     /gene="lacB"
                     /locus_tag="ACGE4H_002147"
     CDS             complement(7083..7598)
                     /gene="lacB"
                     /locus_tag="ACGE4H_002147"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFF8365541.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            complement(7615..8040)
                     /gene="lacA"
                     /locus_tag="ACGE4H_002148"
     CDS             complement(7615..8040)
                     /gene="lacA"
                     /locus_tag="ACGE4H_002148"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFF8365542.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
BASE COUNT         2495 a         1691 c         1398 g         2545 t
ORIGIN      
        1 acgctatatt taagatttca aacaactata ttaataggaa gaaaatttca tttttcctct
       61 tagttctata aaaagtgtgg ctaatttgag gcaaaatatt aaattaaatt tgccacaaat
      121 taatggctcc actattattt taaatcaaga agtagtaaat aatcttattt tttactacca
      181 gactgtcttt agatattagc ttttcgtcac ccactacagt tgacatagac ccacaaaaaa
      241 cttaaaaacc ttgcgatgta agactggcac ggagaagacc aatctatcat cacaaggtaa
      301 gagtattcaa atcttattga agactcaaat aataacaaat tttaattaag gatagtaata
      361 tcatagattt ttctagaagt ttcttgaggt gaaattttcg ttacctgtct tttatcttct
      421 aagaaattac cttcttcaag tgaagtggag agaccactcc aaggttccaa agcaatgaat
      481 ggacttttat tagttgtaga ccacagaatc aagttcggaa aatcatcaaa atctactcga
      541 agacctttac cagacttcct tgaccgaagc gttacgctac gagacttgag ctggtcaagt
      601 gtaatggcat catgggaaaa aagtgagtag tccaaatcga gaattttttg attttccaaa
      661 aaaggtgtcc gatcttgcaa atctaataat cctgtctcag gaaaactctt tggtatcgag
      721 caagactcaa cctcactaaa ttcaatataa taatcctcat agttttcacc ttctactaaa
      781 gaactattga atgctggatg ggctccaata aagtaaggca ttgttttttc catttccaag
      841 ttcgttacct gaaattcggt tctaattgat ttaccattca aggtataaac aactctcagt
      901 tcaaactgat ataggtaatt atcaagcatc tcagcatttg gtttaatact aaaggtcacg
      961 ctattatcat tgacttcttc cagaataaat tcttcttttc gaacaaaacc atgtctacga
     1021 ataagacctg taaaaaaagg tctttcttga ggtctataaa tagcccaatc atttcgtaaa
     1081 ctaccacaga taggaaatag aataggcgcc tgtccattcc aataatttgg atcagcttgc
     1141 cagagatact ctatcccatc ctcgtcttta atcgaagtca gttgaccacc aagtgtttta
     1201 aactgaacag taagatactc gttttttaat tcgatagtca caactaattc ctttcttttt
     1261 attattggaa aggcaaataa tttacctcga agtgaatagt ctcacgtatt tctacatcga
     1321 gtgattgtaa aaattcccat tcaaatgctg ataactctaa atcagaatgg caaatccact
     1381 cttttccagt taaaacatac catttaccac attttagcaa cgagggtagc ttcaagctca
     1441 ggaatcatag gacaatcttc gaaactaaca atatatacct tttcaaagcc tggtctaaat
     1501 aagtgttgta ctttttctaa agtatcggat aacaattttt tacattttca agtaatatta
     1561 ctctatcact tgagtttctg ctactttctt ataccaatga gctgatttct taggatagcg
     1621 ttcttgcgtg tcaaagtcta catagaatag tccataacgt ttttcgtatc cgtttgacca
     1681 tgagaagaca tccataagtg accagatgaa gtaaccttta acatttgcac catccgcaat
     1741 tgcgtctgat aaaacttcca agtgtttctt cacgtaatca atacgaccat catcataaac
     1801 ggtattatct acaaactcat ctttgtatcc aagaccgttt tcagtgatgt aaatcttctt
     1861 gtagtttgga taatcatttt tcaatcgcat gatttggtca tacaagcctt caggataaat
     1921 aatccagtcc caatccgttc taggcacata atctggtgcg attcggcgac caacaccttt
     1981 aatctggtac ttagaacttc ctttttcacc cttaccattg tgaatgattt cagtctcacc
     2041 atcaaaggct tgcatccaat cactcatgta gtagttgata ccaaggaaat cgttcaaatc
     2101 tttagctgct tcaagtgctt ggaagtcttc atcacgaaga tcaagttctc caccattttc
     2161 agctaggatg tggttgaccc cttccatcgt tttatctgaa tagtgtccaa ggtaggttgc
     2221 atccaagata aatttgttat ggatgatatc ttctaactca gcggcacgaa catctgctgg
     2281 attttctgga tcataagggt atttggttgg caacgcatgt actacaccaa tttcgccttt
     2341 ataaccttta tctttataca atttcacagc acgtgcatga gaaaccatca tattgtggtg
     2401 tgattggaag actttggcaa gatcatattg aatgcctgga gggaatttac caaccaagta
     2461 ttgaccatct ccaattgggc caatttcatt gaaagttgtc cagtagttta cttctgggaa
     2521 ctcttcaaaa cagaaagcgg cataatctac aaagtgttct atattttcgc ggtttaagaa
     2581 atctccatct gagtgaagaa cttctggtgt gtcaaagtgg tgcaaagtca cgaatggctc
     2641 aacatgacgt ttgtggcact cggcaaaaag attatggtag aattcaacac ctttttcatt
     2701 tacttcgcca tagccagttg ggaaaatacg agaccaggca attgaaatac gaataccatt
     2761 aacgccatat ttttcagcta attctaaatc aacaggatat ttatggtaaa aatcacttgc
     2821 aggctctgct gtgtaccaat aattgtcttc aagatactta tcccatgcaa ctgggccttt
     2881 accatccgtg tgagtcgcac cttctgcttg ataagcagct gtagcaccac caaaaataaa
     2941 atctttagga agtgttttag tcataactat aaatcctttc aaaaaaagca tttgagacga
     3001 tattctgagt tccggaaacc aaggaaatga aatgcaatga tatggttaac aatattataa
     3061 ggaactgaaa gaataatctc gtctcaaata acttttacaa atttattaat caaactgttg
     3121 ttgaacaaat gcaagagcac cttgtccatc acgtgtcaat ttgatatatt gagctccttc
     3181 agtttttgca agtttaatgc ccaatttatc cgtttcagcc ttcatatcat cgaagtttga
     3241 agcgacctgc ggagccaaga taaccaagtc aaactctggc aacatttcac ggtgagcacc
     3301 gtaactaccc gctgccgctt tgactggaac attgtattct gctgctgctt tgttcaaggc
     3361 attggcaagc aagccacttg tacctccacc tgcacaaaga acaaggacat tagtttcttt
     3421 tgtgatatta ttgtttgctg ctacatcttc tttagcaacg cctgcttttc caagaacggc
     3481 atctgcttta gcagtattga agtttgcagc tactttttct ttaagagcat cgtttgtttt
     3541 accagaacgt tcttcttcaa gaatttgttc atcgtatacc ttaacaaatg ggtaataaat
     3601 gatagtatca acaactacca agagtccggc taagataaat gatagaactt ggaagttagt
     3661 accgagtaca attcccaacg gaccaggagt aacccatgga aggttggcag agaatgagtt
     3721 catgttcaat gtgtcaacaa agaatttaaa gatccaaacg ttgacaatcg gagcgaaaat
     3781 gaacggtaca aagaaaatcg gattcaatac gattggtgca ccaaataaga ttggctcatt
     3841 aaccccaaag aatgttggaa caactgaggc acgtccgatg gcacggttac gttctgattt
     3901 acaaatccac atgaacaaga atggaacaat caatgtagct cctgttccac ccatggtaac
     3961 aataaacatt tgagtaccag aagtgataac tttatctgca tgttgtccag cttggataag
     4021 atgcaagttg gcatcgatat tggcataagt gatagcagcg attgctggtt cgacaattga
     4081 aggaccgtgg ataccaacaa accagaagaa ggcataagca ccaaagataa gtgtaattcc
     4141 aaggtaacca tctgcagctg agaagagagg agcaagaagg gtaccgattg attctgcaac
     4201 ggttacacca agagttccct taacaatgag ttcaaatcca taaaggagaa caaccgctac
     4261 agtgaatgga atcaagtctt taaatacttg agagatattt ggtggaacct cttcaggcat
     4321 acgaatagta acattatttt tcacacagac cttgtaaaca ttaactgtca caaaggctgc
     4381 aataaatgct gtcaaaagac cttttgttcc cgtgaaggct gtcaagaagc ctccttcttt
     4441 tgcaggctca gctgccatca aaaggaaccc taccatagac gctagcatcg tagataagaa
     4501 gttaatctga ttggtcgcag gtaggtcacg gttcattgag tccgtcaaag ctttagcggt
     4561 agtaccacca acaaagaatg ctaggatacc catcgaatag ctataaggag tcatcaagaa
     4621 ggtctcaatg tcttttgacc agtggaatcc ccaagcattt gggacatagg caataaggat
     4681 aaagatagat gagaaaagga tgactggcat accagcgata aatccatcac gaatagcacg
     4741 aagatagatg tttcgagaga ttttctcaaa gaacggtttc cctttctcaa taagttcaat
     4801 gagtttatgc attattttgc tcctctttta taaagttcga tgaggtgatg aatcacatct
     4861 tttaataaga cagtagtcat taagtggtcc tgaccatgca tcatggtaac gctgtatgga
     4921 agttcctccc cagaagcttc tcgagccaac atagctgtct gagaattgtg agcctctgca
     4981 atacagcttc ctgcttctac tacaagacta tctgccttag cgaaatcacc attttcagcc
     5041 gctttaagcg cttctaaaag tttagagcga gcatctccag cataagcaac aatttcaaac
     5101 cctaagagag tcatctcttc tctgttcata acaatatcct ccgttttatg tttttactat
     5161 actttatcag tccatggact agctgtttta acaagaactt tgttaagttc atcaatattt
     5221 tcgaatccag tagtacgcaa ccattcacgt gctgcttttt caccttcttt gatgtaaggt
     5281 tctacagatc ctgcccaagt agcacgtcca caaagcacac cattaaactt ggcacctgag
     5341 tcgtgagcaa attgcaatgt ttcttggaac aatttagcag atacacctgc acttaggtag
     5401 atgtatggaa ggttagtcgc ttcttcttgt gctttaaaga agtcagcagc ttcagcttta
     5461 ctgtaaacca cttcaccatc agcaaaacct tcgacatatt taacgttaac tggtacttct
     5521 actttaagaa catcaatgtt aaagcgtgga tcagagaaaa ctttcatcgc ttcaatgact
     5581 ttgcgaggtt tcacttttgc atattcaaca gaacctgcat ctgagatttc ttcatcataa
     5641 gcaaggattt ccaagaagaa aggaatatct tcagcgacac attcagaacc aacacgttca
     5701 atataagctt gtttttgttg gttaagttca tctgagctgt ctacatcata gtaaagcaag
     5761 aacttaacgg catcagcgcc ttgctctttg atacgttttg cagaccaaac atccaagcag
     5821 tcaggcaaac gttttgtact tgatgtatca taacctgttt tttcataggc aagaagaaga
     5881 cctgcttctt tatccaatgc ttttgttgct ggaagtccat attcagggtc aagcaacatt
     5941 gaagaagcgt attttgtaag ttcatcagca accaaaacct ttagttcttc catttgagct
     6001 acagttggtt ctgtatcttg atactgtgcc atcaaacgtt tcaaagcacc acgttggtca
     6061 aatgccaaag ctgagataac accgttttta tctgaaattt tttccaaaga tttgcgtttc
     6121 tgttctgtaa gtaccatttt tatacctctt ttactccaat ttgtgagttt agtgtttcgt
     6181 aattagtcat attcacatgc cctgtcattg tttcttgagc atttaacata cccaatgtca
     6241 tcgcatgttt taataagtca gcgtcacttt ttttactatt taaagctgat gcaataccag
     6301 ctaccgttga gtcccctgat ccgactggat taacaactgg gatatcagga atatctacct
     6361 tatagaaaac atcaccatgt tttgcaaagg caccgtttcg acctaatgag accactatcc
     6421 attcgatacc actaaaaagt gactctttaa gaacgtcttt gagttcttca atatcttttg
     6481 ttacttcttt tccaagcaac tgtgaaagtt cctcattatt aggtttgatg gcagtaggtt
     6541 tagccgacga ttttagaact gtttcaagag gtgcacctga acaatccaaa acgactgcta
     6601 ctccttcatc cgaagcaagt tggataagtt tttcataata atcgttggga agtcctgaag
     6661 gtaaacttcc tgaaattgtc acaacttctg attgtttaat aagattgcta tagtggtcaa
     6721 gaaagccttc tgcttcttca tgagaaattg ttggtccttg ttccaaaatt tctgtttggt
     6781 taccctcgtg taagatagca atacagtttc tagtgtttcc tgagatttta taaaaagctg
     6841 gactaacagg agattgttct aattcgcttt caataaattc accaatttta ccacctaaga
     6901 aacctgtagc agtaacttta tcaccagatt catacaagac tctagtaaca tttaagcctt
     6961 ttccaccagc tgtcttacta acatctttca ccctattcac tgtatcaatt ttcaatgttt
     7021 ctagaggata agagatgtct actgaaggat ttagtgtgac tgtcagaatc atttgttata
     7081 ccttaatcgt ggtagtcacc acggtcccat ctttcaagga attctgtaaa gaaatcttca
     7141 tctgcttgat gtgcattatg tgtttcaacg tgttcgattt ttgcaatcaa ttttttattt
     7201 tcttctgttg gtttgtactc agcttcaatg aaagcttcaa tgatgtcatt cataagaaga
     7261 ccaccagtaa tctttccacc gaaaccgata acattcgcat ttaactcttc ttttgcatat
     7321 atagctgatg tcatatcacg aaccaacgct gagcggacac ctggaacttt attgactgca
     7381 ttgttaatgc cgacacctgt accacagata catactccca agtctgcttg accacttact
     7441 actgcttcac cgactttttt accatagata gggtagtgag tacgtgcgtg gtcatatgtc
     7501 ccgaaatcaa ggacctcatg tccttttgat ttcaagaatt ctgatactgc cattttttca
     7561 tctgtgacaa tgtggtcaca tccaattgcg attctcattc cttaactcct tttcttaaca
     7621 cattttgtta agcatatcta ctcggatttg atgacgtccg ccatcatatt taccattgac
     7681 gaaaccttta gcgatgtttt tagcaagctc atcaccgacg atttcagcgc caaccgtaat
     7741 cattcgtgaa ttgttatgtc cacgtgtcat ataagctgag cgttcatctg aaacttctgc
     7801 tgcaaccata cctttaattt tagttgctac catgaatgga ccagctccat aagcatcaat
     7861 tacaatacct aagttttgct catctttatt tacttcagag gcaactgcca aggtaacatc
     7921 aacaaaatct tgtccgtcct tagtcacatc aattacctca aaaccttctt ctacaagaaa
     7981 atttttcaca acatctttta atctcgtacc tttgagatct gcaccaacaa caatagccat
     8041 tttgaccacc tcctaaattt tagaaagaaa tgctacgtag aagtagaaaa cattttgcaa
     8101 aatgttcgaa aaaacgtttt catcatttc
//
LOCUS       DAXWLB010000126         7978 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXWLB010000126 DAXWLB010000000
VERSION     DAXWLB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.127"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..275
                     /locus_tag="ACGE4H_002149"
     CDS             <1..275
                     /locus_tag="ACGE4H_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365543.1"
                     /translation="TEFVYNNKVIFILFLIVCIIVFDVYDLLIHKNENSNIGRIIITN
                     LIVITIGVTTLTNKALISSMILIVFDFIIIYSLLFLIKKVTEYLNR"
     gene            442..1005
                     /locus_tag="ACGE4H_002150"
     CDS             442..1005
                     /locus_tag="ACGE4H_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365544.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGKFYSRNKVTTFKNSISGREDLKGINKNSKFYRRNKELL
                     EKISKRVSELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYVAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            1016..1876
                     /locus_tag="ACGE4H_002151"
     CDS             1016..1876
                     /locus_tag="ACGE4H_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HFF8365545.1"
                     /translation="MSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQLDIVRGLKI
                     SFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSLAFSLFIFA
                     LFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHFQNYYNTTT
                     KLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVELFLFKMDSK
                     WDKYIYRSKEVFLTGSYNETLQESLYDTGAIFNNEPMVLKDFFGDYELVWNPKQLSEQ
                     GAEDEFAEVH"
     gene            2374..2628
                     /locus_tag="ACGE4H_002152"
     CDS             2374..2628
                     /locus_tag="ACGE4H_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365546.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            3026..4627
                     /locus_tag="ACGE4H_002153"
     CDS             3026..4627
                     /locus_tag="ACGE4H_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFF8365547.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTIFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTLILQ
                     TSRGRYGSTKIATSKFVEAKILSTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            4861..5160
                     /locus_tag="ACGE4H_002154"
     CDS             4861..5160
                     /locus_tag="ACGE4H_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFF8365548.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            5295..5672
                     /locus_tag="ACGE4H_002155"
     CDS             5295..5672
                     /locus_tag="ACGE4H_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8365549.1"
                     /translation="MNIDGIYNESDFKKGIVDIDRKEYLFPDEIGLFKIVGETVCRYW
                     GKKGNIILLVDTIEGRKLKFSFWSNTKYCCEPVPKREVDVAKESVRFLPDRSKITLTC
                     ERTSNAKFTRIRDILLSTTSSQS"
     gene            5721..>7978
                     /locus_tag="ACGE4H_002156"
     CDS             5721..>7978
                     /locus_tag="ACGE4H_002156"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFF8365550.1"
                     /translation="MAVVILAEKPDQGAKFASALAGKKLSKKSGKYEFKSEIFGDTIV
                     TWGIGHLVGLSLPEKYEWLPNKDKWHLDNLPFLPKFNELKYEVSKGKNQQFSIVKSCL
                     ENADEIIIATDPDREGENIAYNIFKLCKQSIFNKPIKRLWINSLVQKEIIKGFKNLRN
                     SEETISYFKEANARQIADYLVGMNYTELYSLKLQEKGISGVVSLGRVQTPVNTLVVEN
                     DLAIRNFRPEKYKTIECHTTDKQPKAIFKNSLEYFKLEKFEEDTKKFGLDSASTGIIK
                     KIEVSKKQTEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGNYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNINSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENG"
BASE COUNT         3063 a          941 c         1460 g         2514 t
ORIGIN      
        1 caactgaatt tgtatataac aataaggtaa tatttatatt gttcttaata gtttgtatta
       61 tcgtatttga tgtctatgat ttactaattc ataagaatga aaatagtaat attggtagaa
      121 ttataatcac aaacttaata gtaattacta ttggggttac aacattaaca aataaagctt
      181 taatatcaag tatgatttta atagtgtttg attttattat catttatagt ttgctgtttt
      241 taataaagaa agtaactgaa tatttaaatc gttaggcacg aaataaattt taattgaggt
      301 tgaattatag cggtaaatca tacaaaatag aaatgtagtc ttgttaagcg tcttggctat
      361 ctaattttga atgaggaggg ttgtatatag aaaaagggta gttaaattcg tagatcaaaa
      421 ggctacgaag gagaataaag aatgacagct atttataaag cactaaaaaa ttctaacgta
      481 tcagagtacg ttaagtcgag agtattaaat aataatttag attacatttt agaaaatggt
      541 gttgctgatt taatcagtaa gttaagtgat cctatagata tagatcattt agtggtatgg
      601 caagatggta ctttagaagt atcaatatat ggaaaatttt atagtagaaa taaagtaact
      661 acattcaaaa atagtattag tggtcgagaa gatttaaaag gaatcaataa aaatagtaaa
      721 ttctatagaa gaaataaaga gttgttagaa aaaatttcaa agcgtgtttc tgaactggtg
      781 aatccatttt caaaaaaacg tagtaaagaa ctttttggaa ttttttatga tggttttagt
      841 gcaaaaagta atgtagaaaa atttttttat tttaatgatt cagcttatgt tgcggttaaa
      901 gacagtaaaa gaatccctga agcaacttta gaatatttag agccaattaa attgtctgtt
      961 ttttattcac tttatgagaa agaaattgct gatgaagcgg tttaagggga ggaaaatgag
     1021 taaaaaaata ggcaaagagt cgttatcgct cggattgaaa aaagaaattg ttaaatttaa
     1081 aagtatgtat agagagccgg aatttagact catttttcaa ttagatatag tgagagggct
     1141 gaaaattagt tttagttgtt taggttatct ggtctggcta ttcattgcta gagtaggatg
     1201 gagcaatgct tatagctttt ataaactgtt cgccggaact gatcgattaa aagaatcatt
     1261 agaggtgaaa gaagccttaa atattttagg aagtcttgct ttcagcctgt ttatttttgc
     1321 tttatttatc ttacaagtag gaaaaataat gatgtcaaca aatgacaatt atattaatta
     1381 tttgaagcct tttcaaaaag gaataaaaat catttgtaga cagggcacag ttttatttct
     1441 acttagcctg tatgagcatg aaattcattt tcaaaattat tataatacga caactaaact
     1501 tggtgcattt ttactcctat atttagttag taatatagct gaaaaaatgc tgtttaaaca
     1561 tattgtaaag caagtctttt ctgatattca aaaagaaagc tattgtttaa aaacacctta
     1621 tggaaattta aaaaatgttg agctgttttt atttaaaatg gatagtaagt gggataaata
     1681 tatctataga agtaaagaag tatttttaac tggtagttat aacgaaactc ttcaggagag
     1741 cctgtacgac acaggagcaa tatttaataa tgaacctatg gttttgaaag atttctttgg
     1801 agattatgaa ctagtttgga atccaaaaca gcttagtgaa cagggggcag aagatgaatt
     1861 tgctgaagtt cactaacata gtgtacagaa aaacattatc tgaaagaaga acgccttcaa
     1921 aagaaagtat aagaacgatt gggccgtaca gaagactaac gaaagatcgt aaaaaaaagg
     1981 aaagacaatg aaaatgtctt tccttttttt tgactacaag tagcctgaaa atgaaattcc
     2041 aaaagactac aagtagtctt ttgtggcaaa aataaagagc caacgaaagc taccaacaat
     2101 cgttgactcg taagactgtt cgtagtaaga aaaatctttg gcaatccacg aagtctttat
     2161 ttcataattt atataaatga ttatacagtc attttcctga atttgcaagt aaaaaagcta
     2221 agaaatattg tttaagcgtt tccttaaaag aatctttaaa taaatttttt tccaattgaa
     2281 aacatgacta caagtagact atttctttta ttccaagttg actacttgta gtcaaggtga
     2341 tataatatgt atatcaagaa aggagaaaca aaaatgtata acgtaggaga tcaaatagat
     2401 gagatcgtaa aaataggtaa tgttaaactt gttaaaagta agtactatat ttcagaaact
     2461 gaaataattt ttaatactga atcaggatgt attctaattt ggaaactgcc ttttcccaaa
     2521 gacgaaacaa cactgaaaaa aataaatact ttaaaaataa gatattgcat tcatgaaatt
     2581 attgataaaa atacttatgt tataaatcaa gtaaaagtga tggggtaaaa atcttttaaa
     2641 aaaaactctt gttaaaagta attctattag cttataatgt tggtattagt tctgaagcca
     2701 aaggaacact aaaaaattct agtagaaaag ttagttacag actctctact atgtatgaat
     2761 gtaacgaaaa aaatagcgaa aaagataatt aaataagact accaatctta tttaatggaa
     2821 cttctgtagt aagaaaagcg atttggcaat ccacgaagtc caccaagatg agctaccaac
     2881 tcattttaat ttttgcacgt ttaaagttta gcatttaagt gcttactcta aagacatagt
     2941 gtaattttag gactccttat tacggaatct aaagttaccc tatgtctttt ttgctattca
     3001 aaattgatag caaggagaaa gtgcaatgtt gaatagttgc gagtatgcgg acgttttagc
     3061 taaaattctt aaaaatggaa ttaaacagtt taatagcaaa aatctagttg cttttaaaaa
     3121 gaatagcaat aagattggtg ctattgccgg atttacttct aaaacaaaca tgataaagac
     3181 taaaggtctt atatatcgtt ctattgatcg tgtcttggaa gattcagatg tattaacaca
     3241 ctggactcct aacgtgtatt catggcttgg tggcggtgaa aacaaaacaa tttttggtca
     3301 caaagaagct aacttaattc agattaatac ttttgtagca gatattgact atccagtagg
     3361 agaaaagaaa acggatttga atttactagt tctttatcta ttggaagaag ggttattgcc
     3421 tacactgatt gtagatacgc caaaaggtta tcattgctat tttctgattc aaaactataa
     3481 ccaagaaact gattcatttg ataaggcaag ctatatttct aattcaaatg attttaaatc
     3541 tttgagagtt gctaaaagaa tatcagaaaa tatcagaaaa tctatccaaa atcgtttacc
     3601 acaagtcgat atgggctgta atcattttgg tatttttaga tttccaacta aaaaaaatat
     3661 tgtacattat gaacctaatt tcgtagatac gtttgaaggg tatttgaaat ggtcaaaaga
     3721 ctttgaagaa aaagaatatg ctttaaagag gtctaacttg tcattattag ctcctaattc
     3781 tactggcaag aaagggtcta aacgacaaat tgatactaaa tggtacgact ttttaattca
     3841 tacggatatt gctgaaggag atagaaataa aactattttt accttagccc tggcatgtaa
     3901 atcaagtggc ttatctattg gcgagtgtat ggatcaattg gatcaacttc aattcaataa
     3961 tgatttgtcc tattctgaag ttgagagaac aataagaagt gcttatcgtg gcgattacaa
     4021 aggtgctaaa agttcatatg tcaatgaaat tatcgaaaat tatgctacac ctaccgagtt
     4081 tgaaaaatac tcaataaatg taaatagaca agctgttaaa cgtaaaggga atcgtgtcga
     4141 tccgtctaat tgggtgaaat ttgctaaacc tagagaagaa cgaaaatata gtcattttga
     4201 agaaagtaaa gctgatttat tagcttactt agaaagaaaa caaagatcat tgaaaaaaaa
     4261 tgagttgttt ttaagtgtct ctatgggaac tatatctgaa gaaacacaaa ttcctgtatc
     4321 tacgttaaag gtgattttaa aagagcttag aacggcgaat acattgatat tacaaacgtc
     4381 tcgtggtcgg tatggatcga ctaaaatagc aacaagcaaa tttgtggaag ctaaaatatt
     4441 aagtacagtt attcagaata aaaaagataa acaagtattt gtcttagaat tattaggaac
     4501 agcaaacagc cacgtagaaa cgattttaag acgttatgaa gacaaaacag taccgttcca
     4561 tgcgaacata ctgaaagaaa gagaaaagag caaacatgcg ccaattagag gagctaatac
     4621 aggttagagt tctatttatt tttttgattt ttaaatagct aaattgccaa ttaacatatc
     4681 tagtgtttct gcccactcct attcctgttt taacgtatac taatgattag tagttagtac
     4741 tctcttttat gtatggagtg tatagaacta tttttacttg tgtcttttcc cttaatccac
     4801 gtgtacggaa tacagcttat aactgcgtat gcagttatgt ttgaaggagg aagattttct
     4861 gtggtaatta agaaatatgt ttactcacac ccgatcaatg tatttattat cggtaagcta
     4921 ggtttatctg ttgaggtgtt ttgtgagtta tatggattta aacaaggaac tctatcgtct
     4981 tggattacta gagagcgtga ggtagctgat ttgcctattt cttttttgta tgctttgtct
     5041 ctagcttcag gagggaatat gaatgaggtt tatgattgct tgtgtgagta tgaaaaagag
     5101 tataaagagt atttgaaaat ttcagcccca aaacggaata aaaaatatat tgaagattag
     5161 agaaagaaga aaagaataat ttgttttttg actacaagta gactgatagc tactctgttt
     5221 tttgatagtt tgatattggc tgtttttttg ttagaattaa tatatatcag gtaaataaaa
     5281 aaagggggct attaatgaat attgatggga tttataatga atcagatttt aaaaaaggca
     5341 tagtagatat tgatagaaaa gagtatttat ttcctgatga aataggttta tttaaaattg
     5401 taggtgaaac tgtctgtagg tattggggga agaaaggaaa tataatttta cttgttgata
     5461 cgattgaggg aagaaagtta aaattttcgt tttggagtaa tacaaaatat tgttgtgaac
     5521 ctgtacctaa aagggaagta gatgtggcta aagaaagtgt acgattttta cctgatcgat
     5581 caaaaataac tttaacttgt gaacgaacaa gtaatgctaa atttacaaga attagagata
     5641 tattactttc gactacaagt agtcagtcat agagtaagta cttttggtta aaaatcaatt
     5701 gaagaaagtg gaggaagtat atggcagtag ttatattagc agaaaaacca gatcaaggag
     5761 caaaatttgc ttcagcattg gccggaaaaa aattatcgaa aaagagtggg aagtatgaat
     5821 ttaaaagtga aatatttgga gatactattg ttacttgggg aataggtcat ttggtaggat
     5881 tatcattacc tgaaaagtat gagtggctac cgaataaaga taaatggcat ttagataatt
     5941 tgccttttct acctaaattc aatgaattaa agtatgaagt ctctaaagga aaaaaccaac
     6001 aattttcaat agtgaagtca tgtttagaaa atgcagatga gataataatt gctactgatc
     6061 cggatagaga aggagaaaat attgcttata atattttcaa attatgcaag cagtcgattt
     6121 ttaataaacc gataaaaaga ttatggatta attctttggt tcaaaaagaa attatcaaag
     6181 gatttaaaaa tctgcgaaat tctgaagaaa cgatctctta cttcaaagaa gctaatgcta
     6241 gacaaattgc tgactatcta gtagggatga attatacaga gttgtattca ttaaagttgc
     6301 aagaaaaagg tatatcgggt gtggtatcac tcggtcgagt tcagacaccg gtaaacacac
     6361 ttgtggttga aaacgatttg gcaattagaa attttagacc ggaaaaatat aaaacgattg
     6421 aatgtcatac aaccgataag caaccaaaag caatatttaa gaatagtctt gaatacttta
     6481 aattagaaaa atttgaagaa gacactaaaa aattcggcct tgattccgcc agtactggaa
     6541 tcataaaaaa aattgaggtt agtaaaaagc aaacagaacc acaaaaattg tttagcttgg
     6601 gtggtattca agaatacgct aataaaaaat ggaaatattc tgctaaaaaa acagataaaa
     6661 ttatccaaag tctatatgat cgtggatatt tatcttatcc aagaaccgat agtgaattga
     6721 taactacaaa tgaatttgag tatttaaaag cgaatttaga cgtgtataag agtgttttag
     6781 ggatcagtat tgatacacct tgtttagagc ctaaaaagcg atttgtagat aatgacaaag
     6841 tattagagca ttatgcacta ataccaacaa tggatattcc tgatatagca aatctgacat
     6901 tagatgaaaa aaatatttat gagatcgtta ctaggcgtgc gtgtctaatg tttgcagaac
     6961 cttacaagta cgaaaataca aaagtcacat tagatgtaaa cggcttagaa tttaccacga
     7021 gtggtaatgt acctatatct ttaggttgga agcaagtaga tactaattta gatgaatctg
     7081 atgataaaga ggaaacatta cctaaatttc atgaacaaga gacaataaat atagatatta
     7141 gatttattga taaattaact aaggaaccta aaaggttgac tgaaggtaaa ttattaggta
     7201 aaaccggtat tatggcgaaa ttagggctag gaacgcccgc tacacgttct aatattgtag
     7261 acactcttct aaatagggaa tatattaaga tagaaaacac gaatgtttat cctactccaa
     7321 agggatattt attatgggat ttgacaaaga ataaagacct attgattgga aaaccagaaa
     7381 atacagcgat atgggaagaa tcacttggga aaattgcgca aggtaactat acacaggaag
     7441 cttttttaag aaatatctat aactatatca ataagactgt ttcagatttg aaaaaagtag
     7501 aatttacttc ggattattta gaaaagactg taaatcaaaa taaatttgat ataggtagtt
     7561 atcaagtgga agaaaagaaa aaagtttatt ctattttaaa tattaatagt ggtgaacagt
     7621 tcgtgatatt taaaaatata tcaggaaaag caatcaataa aaaagttctt caagaactat
     7681 tactaaataa tcgtacagat aaagtccttt ccggctttaa gtcaaaagaa ggaaaagctt
     7741 ttaatgcaag attacttttc gatccaaatg tgatgaaagt ggtaatggag tttgaaaaga
     7801 acagtccaaa aggcgataaa aagagcaaac acgatacagt aaatggttat gaaatagttg
     7861 aaaaagcaaa agtatttgaa ataaaagaat tagcaacagg tgaaagtttt attttctata
     7921 aaaataatag tgggaaaact ttaagttcaa aaacagtcaa agaattgtta gaaaatgg
//
LOCUS       DAXWLB010000127         7907 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXWLB010000127 DAXWLB010000000
VERSION     DAXWLB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.128"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(794..3943)
                     /locus_tag="ACGE4H_002157"
     CDS             complement(794..3943)
                     /locus_tag="ACGE4H_002157"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFF8365551.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3957..5174)
                     /locus_tag="ACGE4H_002158"
     CDS             complement(3957..5174)
                     /locus_tag="ACGE4H_002158"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8365552.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(5164..6759)
                     /locus_tag="ACGE4H_002159"
     CDS             complement(5164..6759)
                     /locus_tag="ACGE4H_002159"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8365553.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6884..7669
                     /locus_tag="ACGE4H_002160"
     CDS             6884..7669
                     /locus_tag="ACGE4H_002160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFF8365554.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7662..7904
                     /locus_tag="ACGE4H_002161"
     CDS             7662..7904
                     /locus_tag="ACGE4H_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365555.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         2352 a         1587 c         1290 g         2678 t
ORIGIN      
        1 taccatttaa tcaaaaaatt agctcatatg aatatattag aatgtaaatt agactggtca
       61 aaatctggtc aaaaaagagg acgaatacag gtagtcctct tttttgttat tttgatattc
      121 taaatcattt tttattggat tattcccttg ttcgatatag acccaaattg atctatcaat
      181 aattatcaaa ctgagtcata tagagacaaa aatttcaaaa atgacctgtt aaatggtcat
      241 cgaatatcat ttaaaatcaa tacgacggag tcagacacgg agtcaaaaca agcccgctga
      301 gagtaaaaaa tatacagtcc cataggtaaa gtgccacagg actgtatatt tttttatttg
      361 agattacctg aaatgattcc atttaatttt tatctcattt tttcctacta gttagtatca
      421 aatactctcc tattttctca ataaactatt ttgggttaca tggtatctga aacagctaaa
      481 accttgtcat tatagcaaaa aatcacttta tctccgtttc atttcagata catttcactt
      541 aagcggattc caaaaaaaca aaagaaaaag gaacccctag ttagcctatg ctttttattg
      601 gttcctctca aaacacggtc atataacaac ggggtggggt aaatgaatga aaaaggtgga
      661 ggaaacatta ttcaatagct gtcttaaata cttcgttata aaatgtgaac aaataaaaga
      721 catctatccc tattttctac acttccactt gactaccatc tttgaatcgt acaattttct
      781 taactcttct cgtttacctc aattcattct caagtggaat caactcatca tctaacattt
      841 tacgccatgc tcgcttcatt tcttgtgggt atttaaatgg cttcgcttct ggatgtttgg
      901 ctttatatcc ttcgtaatcc ttactgttga taattgcttt catattagga attggatctt
      961 cacctggtag atattgcaga ttagaagcat gtaattcttt ctcagaaaca aaccactcat
     1021 tggaaaagac atgaatcaaa tcatcactcg cttgtgtaaa atagcgacgt ttaatgacaa
     1081 agatatcttc attactatct aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa
     1141 cttctttgac tccttctgtt ttattcaatt tagccaaagc tttttcaatt tcttctcgtg
     1201 catctgaact attcgcagca taagttccta gtaattgatc aatataagta gaatcaatat
     1261 catacaactt cgtggtattt tcactataaa attcgatcgt tgaaaaatct tctccctctt
     1321 tttctggttc ttcctccatc aatgaaccct tcacggtctc gtacacgcca atttgttctt
     1381 ccaaaagttg aatttgattg ttcaaagttg aagattgcac catgtcatca ttgtaatcat
     1441 cataagtcac taacgattca tacgcacgat tcaattcttg aaatgctttg acgtactcga
     1501 tacggatttc aagtggcgta tgttcatcaa tggattccat ggatgaaacc tcttttaact
     1561 taccatatgc ttgcttgaat ctttttttgg attcttggta acttggataa atcaaacccg
     1621 attcttcttt agcttctgaa tacagttttg tcgcatcctc gacattcttc tccattgtcg
     1681 ctggctttcg gaaagtaaca attagtcctt ttgttttttc aggatacgta cggttcgtac
     1741 gtgaaaaggc ttggatcaat cctgcatact ctagattacg atccacaaat agggtttgaa
     1801 tcgtaggggc gtcaaaacct gtcaatagac gatccactac gattactaaa tcgatttgtt
     1861 tcccaaattg tttgaattcc gctctttttc gtgccaaacg attattgatg tctccgttat
     1921 aacgctcaat gtctgctagt gaccaggcag tatcataata ctgattatat tcctcaataa
     1981 ttttttgcat ttcttcttgt tgggcacttg aatctcggct attttcttct aatgaatagg
     2041 taatagcgat tcgtgggaaa tcaggatcct ccatcgttcg tccttcacgg ataggctgat
     2101 tgggaaactc ttttattaac caatcagatt ctttcgtcat ctctttgatg gcccgataat
     2161 actttttggc catatcaatg gaactcgtgg ttaagatcgc agacttcgtt ggacggccat
     2221 ttcgaaaatc aaattttgta taggcattgt ctggacggaa cattttatga atgacttttt
     2281 ggatatgttc ctctgcttca aaaacagtag ggtccaaata actttctttt ttgacagact
     2341 ccatttgatc gatcatacga ttgatctgtt ccggtgaata ctctgcatat gtttctactt
     2401 cttttaattt ccgatagatt ttattctcta atgaagtcgg ctccacggtg ttttcatgct
     2461 ctacttgaaa tcctaaaacc gagccatctt ctaatgcatt cttgatggta taggtgtgca
     2521 agacctctcc atactgatca tgggtcgtac gtgccaattg ccctttggct tgtttccgat
     2581 tctcagggaa aatcggtgta cctgtaaagc caaaccaagt ggatttcgga aacatttttt
     2641 tgatttcttc catattttca gcacttaacg ctcgatgaca ctcatcaact acaaacacga
     2701 tatgttgccc cattaatttt tggaagcgat tggttccttt tttctcttcc tgtttctttg
     2761 catatttcaa cgccgcatct agtttttgac gggttgtaat gatcacaaca ttcgcattgg
     2821 catctgccaa cagagtttca cttaactctt tggcacttcc tgttcccacg atcaagctat
     2881 tcgctttggc attaccagaa gaaaccccag tattaaactc cgaagcaaat ttcgtaaact
     2941 ctgtggtggt ctgattatct aaatctttgc ggtccactaa catgatcgtt cgatcgatcc
     3001 ccgattttcg tgctaaaagc ttagtagata caaaactagt caacgtcttt cctgaaccag
     3061 tcgcatgcca cacataccct gattgatgtt tatttgcggc aataaacaag gcttgaatcg
     3121 catgcacttg ataaggatgt aacaccatga gggttttatt gtcttgatct tcactgacaa
     3181 tcgtataatc cgcaatcaag cgatgggcat ctgggatatt tagcacttgt ttgcaaaact
     3241 catataagtt ttctaccttt ttattatctt tcgttcgcca actaaataaa aacttaggat
     3301 gcaaatcctt cggtaaggca tttgcaaaat accgagtcgt ttgttcattg gaaaccacaa
     3361 acaactgtaa ggtcgaaaag atattgttcc gaaacatgcc ttcttcagca tattttttga
     3421 tttgattaaa cgcctgataa aaaccatctt ttgcagttac ttgttttagc tcgatttgga
     3481 taatcggtaa gccattgatc aataaggtga cgtcaaaacg tcgatcccgc gcttctatgt
     3541 tacttccacg ttttgcaatt tgatggacca cttcataagt agagattcct ccaccaatgt
     3601 cttgattgga ataaaggata agtgagacag aacccaattg cggattttcc cgttcaatcg
     3661 tgatccgagc catcccattt tcacctttta accatttcgc agcatcaaat ggggttttcg
     3721 tccgtagcaa taattcggtt ttgatcgttt caaactcttt atcggtcaaa ggagaatcat
     3781 tcagttctgc ttgattattt gagatgattt tttgacgtaa attgctccaa agatcttctt
     3841 ctgattttag atccggacga tacgtccact gattatgtcc ttccccgagg acacgaatca
     3901 actgttcttc gacttccgct tcctcacggt gactaatttt cattcttctt ccctccctaa
     3961 acaaacatct tttgcaagaa gccttttttc gtttccttca gcaaatctaa cttacgttga
     4021 tgaagagcga tcgtatcatc tagctgcttg aagaaggcac caattttttg ttgttcattt
     4081 atatccggtg gaaactgaaa tgtcctttgt tctataccac ctttagtcac atgtaccatt
     4141 gtcgttccgt tagttgattc tttaattttc tctttatcag tcaatagcca agtatataga
     4201 tactgtttgt ccaattcctt agataaaaat ttaagtttcc aaatatgata atggtagatt
     4261 acacgttctt cattccacat ttcaggaccg aagcttgttg cccataaata taagagatct
     4321 cctttattag catacttgtc ttcctctaat tctaagtttg aataatacca tctatcatta
     4381 gtattaaaat tacctactct taaaacccta tattttccag aatcaagtaa ttcttgttgt
     4441 ttaaaagctc taccattcaa aaaaactacc acttccccca acttccgctc ttcccaatct
     4501 cccgtaaatc ctggaaaacg aacttctggc acttttgctc cgtttttagg gaacattttt
     4561 tgtaagaatc cttttttcgt ttccttcagc aaatctaatt cgtgctggtg aagcatgatg
     4621 gtgtgatcaa gttgtttgaa aaatgaaccg atcttgatct gttcgtcata agttggaagt
     4681 gagataggtg tctttttacc actatcaatt gtataggttc cgacggttcc aatgcctgca
     4741 acgtcacgga tatacttctg aattttgtta tttatgaacg agtagtaaaa gaagtttccg
     4801 tcaattttat gctcattctt gaaccaaatg atgtttccat ccttaaagta aattgggtta
     4861 tcatcagtga ttaataaggg gacacctatt gatccgacac cagttacaag caagtctcct
     4921 tgagaaactt tacctgaatt tttcgaatat tcattatatt tttcttctga aatgtaaaga
     4981 taatcggatg gttcctcatt ttttgcagct gagacaatat cacgagcacg taaaaatcta
     5041 atacctgagt tagtccaatc tgattgatga attcgcttca cagaagtaac atccatcata
     5101 tctcctaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat ttctggtcgt
     5161 gtatcattgc tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg
     5221 tgttgtttgc agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg
     5281 tcgtatcttc ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcgaa
     5341 agtatcgaca tagcgtggga tatttaagtt atagtcattt tctttgattt catcaaaggt
     5401 tgccaaatga gcgtatttct cgacatcttt tctttcagcg taggtttcga ggattttttg
     5461 aatgttttct tccgataatt tattctgatt tttcccttta acaaattcac gactagcatc
     5521 aatgaataat acatctcgag tttgacgatt cttcttcaat acaatcaccg tcgttggaat
     5581 cgatgtacca aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc
     5641 taataatttt tgacgaatga ccccttctgc agcaccacgg aataaaaccc cgtgtggcaa
     5701 gacgatagcc atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa
     5761 atctgctttg gattttggcg ctaatttccc gtagcgatta aaccgagaat catctaagaa
     5821 ggtcgtatct gctgaccaat tagccgaata aggtggattc atcactactg catcaaatgt
     5881 gtaaggttca tctgttggcc agtccttatt caacgtatcc ccattgcgga gattcatttc
     5941 ttcggcatct accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt
     6001 tagttcttgc ccatgatatt taacgttatc tggatgagtt aagtagttac gtacatttaa
     6061 catcaaggaa cctgaaccca tggttgggtc aaagacacta aagaatcgtc gttctttttg
     6121 atccaacgtc acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc
     6181 ggctttcttc cctgcttctg aagcaaattg actgatcaga aattcatacg catccccgat
     6241 cacatcccca tcgtgaccta aaacttccac gtcattcaat ttcttgataa cttcggtaat
     6301 cgtcacattt cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc
     6361 aaataaccca ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc
     6421 gttcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg
     6481 tgagatcgaa taccccaaaa tatcaacgat tgtggcaatc aggtcttctt ttgattcttc
     6541 atctgaaagt aattcacgat acaacattgt ttgtttgctt actgtgtcat attcctctaa
     6601 ggattcatct gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa
     6661 aatcagtccc aataaataat ttttatattc tgacgcatcc attttactac ggagattatc
     6721 tgccgcacta aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt
     6781 atttaaagtt gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa
     6841 aaaagaaaaa atcttattct tgataacaag ctacgtgacg tatatgccta acaaaaatag
     6901 aacttttgaa tcaatcttaa atgaaggttt aaccacagaa ccggcatcca ttatttttga
     6961 tgaagttatc gatgacgttg aagatagtaa gtatcataaa aatttaaaag aactttttta
     7021 ttttgcactc aacaatggac caagtttgtt cactggtata tcagtacctg atagtgataa
     7081 tccattaatt atggcaaaaa tttacttaac aaaatggtgt aataaataca tcagggatag
     7141 gaataaccca gcattaaaaa aacctcttaa aacatttggc gaaaaagatg ctgctttaac
     7201 tactagagta gcagcaaatg ctaatattga tgatcaaaaa gtattgaatg actacttgcg
     7261 tggacatttt ttatatatga gcgctgagaa catgaacgga agtattttag aggaatattt
     7321 agccgaagtg ttagaacctg aaggttggat ttggtgcgcc ggctctgtat atcgagctgt
     7381 tgatttctgt tatttaggaa cctcaccaat tcttttacaa gtgaaaaata aatacaatac
     7441 agaaagcagt tcgagttcag ctataagagt aggaacaaca attagaaaat ggaatcgttt
     7501 aaataagtcg acaaaaattt ctggtttgga tagccctatc cccaattgga aagcattaat
     7561 agaaatgact gaagctagta aggaactggc agctaagtta actgaaaata gttatttagc
     7621 ttatattaat gaaaaatcca cacgagaatt atggacactc gatgactaac aatatcctta
     7681 ttagtatcta tccacgatac atggaaaaaa ttataaacaa acaaaaaaac tttgaattta
     7741 gaaatttcta tcttacctca gatccttata atatcttttg gatttacgaa acaatcccca
     7801 ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat tactaaactt aagaaaaatc
     7861 aacactactt tttaggagac gatgagtttg aacaattagt ttaaaag
//
LOCUS       DAXWLB010000128         7772 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXWLB010000128 DAXWLB010000000
VERSION     DAXWLB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.130"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            69..770
                     /locus_tag="ACGE4H_002162"
     CDS             69..770
                     /locus_tag="ACGE4H_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8365556.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="ACGE4H_002163"
     CDS             complement(1342..2358)
                     /gene="tsaD"
                     /locus_tag="ACGE4H_002163"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFF8365557.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="ACGE4H_002164"
     CDS             complement(2355..2819)
                     /gene="rimI"
                     /locus_tag="ACGE4H_002164"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8365558.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="ACGE4H_002165"
     CDS             complement(2826..3368)
                     /gene="rimI"
                     /locus_tag="ACGE4H_002165"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFF8365559.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3352..4074)
                     /gene="tsaB"
                     /locus_tag="ACGE4H_002166"
     CDS             complement(3352..4074)
                     /gene="tsaB"
                     /locus_tag="ACGE4H_002166"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFF8365560.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4265..5245)
                     /locus_tag="ACGE4H_002167"
     CDS             complement(4265..5245)
                     /locus_tag="ACGE4H_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8365561.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5269..6753)
                     /gene="galT"
                     /locus_tag="ACGE4H_002168"
     CDS             complement(5269..6753)
                     /gene="galT"
                     /locus_tag="ACGE4H_002168"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFF8365562.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6765..7754)
                     /gene="galE"
                     /locus_tag="ACGE4H_002169"
     CDS             complement(6765..7754)
                     /gene="galE"
                     /locus_tag="ACGE4H_002169"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFF8365563.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2167 a         1689 c         1376 g         2540 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg actgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata tttatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactgggc aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgatagat taactagagg aagcgtcttg
      961 tcctcatccg acctagatta ttcgatattc ctcgacaata aacgattaat cagtgagggc
     1021 aatcagagga acggataatg atgaataagc tgtaaaaaat agggctttga caaaagcgtg
     1081 tcacagccct tatttcgatt aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata
     1141 atgttcataa acatgaggag ctgttttttg aaattgttcc ttgtgaatca gagttaaacg
     1201 acaggcttac gaactcttga ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa
     1261 cagccaggtc aaaaacatga gaagccgttt ttcctgggct gttcttatta cttgatgcag
     1321 ggcagatttt tcacaacctc tttattcaga taaaaccaag cttggatcag cattcaactg
     1381 atagctagca aaatttcttt tttgcatttc cacatgtgct gctgctccaa tcattgcagc
     1441 attatctcca caaagtcgaa gagggggaat cactagttct acctcaggga gtttagttgc
     1501 caaagcatcc tgcaatcctt cacgcaaccc ttgattggcc gctacacctc cagctacaat
     1561 cagttgcttg actggataat tttgacaagc tcgaagtgtt ttagtaatca atacatcgat
     1621 cacacttgct tgaaagctag ctgctaaatc atttttgtct agatcttctc cacgctgttg
     1681 tgcattatgc accaaattga taaatgcact ttttaatccg ctaaaactga aatcataatt
     1741 atcttcatgg atcatcgctc gtggaaaatg gtaattatct tttccctgat gagctagctg
     1801 atcgatttct tttccgcttg ggtaagaaag acctaatact cttccaactt tgtcataagc
     1861 ttctcctgct gcgtcatctc ttgtctcccc aatgatttca tatgatccat cttcttgcat
     1921 atagactaat tctgtatgtc ctccgctaac aagaagtgcc atcaatggaa actggaatgg
     1981 tttaaccaga cgggctgcat agatatgccc tgccatatgg tttacaggaa taagcggcaa
     2041 ttggtgtgcc caagcaaaag ccttagctgc agaaatacca atcagcaacg agccgacaag
     2101 tcctggtcca taagtgacag cgactgccga taaatcttct gccgacacac cagcttctac
     2161 cagtgcatct tccaagcata aggtgatttg ttctacgtga tggcggctag ctacttcagg
     2221 aactacgcca ccaaaacgtt tatgactgtt tatttgagaa gcaacgatgt tagataagat
     2281 ctctgttcca tttctaacga ccgctacact cgtttcatca caacttgatt caatcgccag
     2341 aatcaattct tcattcattt catcgtctcc gtttttaact ttctgctcat aatgatcgca
     2401 tcttccgtcg gattgcgata atatgatttc cgtttaccta atatacgaaa cttttctgat
     2461 tcataaagtt ttcttgctgc ttcattagat agacgtactt ctaaaaaaac atgcagcgaa
     2521 tcttttattt gttccgtaat cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt
     2581 tctgaaactg caacagcaat atttgtgatc tcgacttcat ctattacttt cgagtatcca
     2641 ataaagccac agatctggtt atcttcaact aaaagcagat aatcagagtg aggctgttgg
     2701 atgtctgcca aaaactgcat tttcgtccaa ggtgaaccat gtggataagc attttcgcta
     2761 atcatccata cgcattctgc caagtacgct tcatcaaaat ctttttttct gcaaatcaac
     2821 taatctcact cattttctaa aagttttatc atatccaacg catcttcttt tgtttcagtg
     2881 taatatcttt ttttgatcgt tgttgcttga aaacctaact tccgataaag tctttgtgca
     2941 tcttgattac tcatacgcac ttctaatgac atcgtctcac agcgattcat tatagcaaag
     3001 ttctcgattt cgtcaataag cagcgaaccg attcgtttac gctgatatgc cggtcgaacg
     3061 gcaatattcg tgatatggca atctaaaccg aacgctcttg aaccgataaa accaatcatt
     3121 cttccattat caaagatacc tagatataga tgaggaagcg gtgacttcaa ttctgatata
     3181 aaggcacttt ttgtccatgg caattcgcca ttatagactt ctcgttcgac atccagcagc
     3241 tctttgatat cttctgtagt caattctctt agtacgtaac gacgatcgat ttgttcgatt
     3301 gttttttctg tataaatcat tttatcttga aaaaagcttc tggcatacgc tctaaatttt
     3361 ttcaacataa ctttcgtctc caggtgtgtg cgtctccaac catttttctt ctgcttctac
     3421 ccgttttaaa tagcgaggaa gaaaagcttg tttctctgca actggttcag ctaaagcacc
     3481 taattgagcc acagttacac cacttggaat atcccatgct ggaactgtat taatcatcgc
     3541 actaggcaat atttccttta tttcatagaa gaatttgtga caatcactcc cgacgaaata
     3601 aatagcttca ttcagctttt gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc
     3661 ttcaagtaca atttctaatc tattttcttt ccagcggtat gccccagcgt atacattttt
     3721 tcttcgagca tcaaacatgg gcacaataat tttatccaca ccgatgcaat tagccgcgat
     3781 ggttttcaga ctagatactc ctactagctc tttatctaat gtataagcaa tcgtttttgc
     3841 tgtcgttaca cctatacgaa gccctgtata agaaccggga ccatcagaaa ccgcgattcg
     3901 gtcaatgtct tttggactta tttgaagatc tgcaaacaat tggtcgattg caggcatcag
     3961 tgttacactg tggttgcgtt tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc
     4021 taccacaccg atcgccaaag tttggtttgc agtatctatt cctaatgtaa tcatatattt
     4081 gatcctttct gctatgacat tctcgccttc cattgtagca tactttttta ttctgaggat
     4141 gaaaatacca aaggcaaaac tagtacaatg atacatgatt atctgctggc gggcaaattt
     4201 tgccccttcc tgaaacacaa aaaagctgcg gtatcacttc atttccccac agcttttcca
     4261 tcttttattt cgttgattct cgaataatca actctgtttc cacaataatc tttcttgaaa
     4321 ccggtgcttt ggaattactt aattgaagaa tcgtctccac tgctagctct cccatccatt
     4381 cggtataaac ttttacagta gaaagagagg gcgttacata tttcgctaca ctgacatcat
     4441 tgaaacccat cacagaaata tcttctggta ccactaaacc tttttcttga atcgctttca
     4501 gtgcgccaat cgctaatgca tcattagctg caaaaagcgc atcaggaaca tgcggattcg
     4561 tttttaaata ctgcttcact gcttgatatc ctgactcgac agaaaaatct gcttcgataa
     4621 ttggaccttc gtcgtagcct ttttctgcca aaacttgttg aaatgccata attctgggat
     4681 cttttagccg ctgatggttt ttcttcgtat attctgttcc agcaagcata gcgatatgtt
     4741 gcgctttatg ggagacaaaa tgttcaatga ctttttgaac acttgactta aagtcgacaa
     4801 ctagagagtc gattccttga gaagtgccgt cagaatctac caaaagcaag gcattttggg
     4861 atcctttcat ttctaataac tgagcttcgt cgaattttcc tagtgcgatc gttccatctg
     4921 tcggaagatc actaatggaa tcccacgttt ctttcaataa agtaacgttt gcttcttctg
     4981 cctttttttc gattcctaga cgaatagata aataataaag atcttcaagc tcttccgtat
     5041 cgtcgtacca ctggatcagg cgaagcgttt gcttagtatt tgctgcattt ttacgatgtt
     5101 tcgtataatt taagcgttct gctgcttcaa aaattttttg ctttgtggct aagccaacgg
     5161 aaagatcagg atcatagttg agaactcttg aaacagtagc aggagaaact cctgccaact
     5221 gcgcaatgtc tttaattgtt gccatttgtc tctcctcctt ttttacgttt ataaaattgc
     5281 agtaaatcgt tcaaacgctg cctgtccttt ttcatctcgc ttgaatactc cagcgtcttc
     5341 cagtacacgt gcaaaaacct gacctacaga tttttgtacg acatcggtga ccgtatcttt
     5401 cgtcaaatca ggatgttctt tttttaattg ttctgcccac ggacgatgat attcagccat
     5461 ctggttttct tctcctaata agaaatgctc tacttctact agttctggtt tcaaacgagg
     5521 tggtaaaaca gcaagcccca ttacttcgat caacccaatg ttctcctttt tgatatgttg
     5581 gacatcttta tgtggatgaa aaatcccatc aggatgttct tctgaaacat tgttgtctcg
     5641 aaggacaagg tccaactcaa agtaattgtc ccttcttcgt gcaattggcg taatcgtatg
     5701 gtgtggtgta ccatctgctg aaaaagctct gatagatact gtttcatccg aatatgcttt
     5761 ccacttttcc aatactgagt tagctgcttc aatcaacaat tttttctctg ttgattgcaa
     5821 acggatcaca gacatcggcc atttcacgac tcctgcgatg acttgcggat aatctgctag
     5881 tttgataagt ttttctatcg gtgcttttgc cataggaaat tcatgacgtc ctgcctgata
     5941 atgatcatga gaaagaatcg atcctccaac aatcggcaag tcagcattcg aaccaacaaa
     6001 gtaatgaggc aagacttcag taatccgaag cagtctagca aatgtttcct ttgtgatttt
     6061 catcgggcgg tgttgttctg ataaaatgat cgaatgttca ttgtaataag cataaggaga
     6121 atattggaat ccccagcttt caccgtctag attcattcga ataatccggt gattggtgcg
     6181 ggctggatag ttcaagcgac ctctatatcc ttcgttttcc atgcaaagca tgcaggtagg
     6241 atagtctact tttaccgcct cgcgttctgc tgcgatttgt tttggatctt tttctggctt
     6301 agataaattg attgtgattt ccagttcacc atactccgac tctgccggaa atttgatgtt
     6361 ttttgcgatg gctcttgttt tgatgtagtc atttttctta cttaaattaa agaaatactc
     6421 agttgcttcc tctgggcttt tcgaatagtg ctgtgcaaag aatgcattga cgactgaagg
     6481 tggcggtgtg agaaaatcca ttaattgcgc ttcaaaaatc tcttgttctg ctggcaattc
     6541 tgaaagaaca ccattttctc ttgccttagc gacaagctga tccaataagt ctacagattc
     6601 ttccttaact ggtcttactt caatgctttc tagagcatct tctcctacca tccctaaaag
     6661 acgattctgt aaatataagc ggtccatttc catccatccg ccagcttcga tcgcaagtgt
     6721 ggcaaaatca caaatcgttt gactgattga catatctcat tcccctattc ttcataacca
     6781 tttggatggc tgacatgcca atcccatgct gttttgataa ttgctttgat atctgtgtat
     6841 tcaggtttcc aacccagaac ctctctcgct ttttcacttg aagcgactaa tcggctagga
     6901 tctccagctc ttcttggtgc tatttctgct ggaatttctt ttcctgtcac ttctctagct
     6961 gcttctagca tttccttgac tgagtatcct ttgctgctgc ctaaattgaa gaagttgctt
     7021 tcatttcctt ctttcaagta ttccaaagct aaaatatgtg cagcaatcaa atcttctact
     7081 tgaacataat ctctgataca tgttccatct ggtgtatcat aatcgtctcc ataaacagcc
     7141 aatgcttttc tttgaccaag agctacttgt aaaatgattg ggacaagatg cgtttctggt
     7201 gtatgatctt ctccgataga tgcatctgct tttgctccgg ctacattgaa gtagcgcaaa
     7261 gcgacatagc gcatgccata tgcttggtca caccacttca tcattttttc catcatcaat
     7321 ttactttcac catacggatt tttaggatta gctggtgtat cttctgtgat tggagactct
     7381 tttggttctc cgtaagttgc tgcagtagaa gagaacacga ttttattcac attgaactcg
     7441 tgcatgactt caagtaagat ttgcataccg tatacgttgt tgttgaaata catcaatggt
     7501 ttttcgacag attctcctac taaagagctt gcagcaaaat ggattactcc ttcaatcggt
     7561 tccttttcaa atacggaacg tagaaattct ttatcccgga tgtctccttc ataaaaatga
     7621 gcatctggat gtactgcttg tttgtgacct gtgagcaaat tatctaccac agctacttga
     7681 tacccctttt gaactagctg gtctacagcg tgcgaaccga tataaccggc tccgcctagt
     7741 actaaaattg tcataaaaaa cactcctttg at
//
LOCUS       DAXWLB010000129         7698 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXWLB010000129 DAXWLB010000000
VERSION     DAXWLB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.131"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            544..1107
                     /locus_tag="ACGE4H_002170"
     CDS             544..1107
                     /locus_tag="ACGE4H_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8365564.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="ACGE4H_002171"
     CDS             complement(1386..1673)
                     /locus_tag="ACGE4H_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8365565.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="ACGE4H_002172"
     CDS             complement(1663..1992)
                     /locus_tag="ACGE4H_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8365566.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="ACGE4H_002173"
     CDS             2297..2626
                     /locus_tag="ACGE4H_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8365567.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4H_002174"
     CDS             complement(2913..3398)
                     /gene="vanZ-A"
                     /locus_tag="ACGE4H_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8365568.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="ACGE4H_002175"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="ACGE4H_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8365569.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="ACGE4H_002176"
     CDS             complement(4890..5498)
                     /locus_tag="ACGE4H_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8365570.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ACGE4H_002177"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ACGE4H_002177"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8365571.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="ACGE4H_002178"
     CDS             complement(6528..7496)
                     /locus_tag="ACGE4H_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8365572.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2351 a         1532 c         1280 g         2535 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaat
//
LOCUS       DAXWLB010000130         7658 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXWLB010000130 DAXWLB010000000
VERSION     DAXWLB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.132"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..545)
                     /locus_tag="ACGE4H_002179"
     CDS             complement(<1..545)
                     /locus_tag="ACGE4H_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFF8365573.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPE"
     gene            complement(547..1311)
                     /locus_tag="ACGE4H_002180"
     CDS             complement(547..1311)
                     /locus_tag="ACGE4H_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFF8365574.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1319..1996)
                     /locus_tag="ACGE4H_002181"
     CDS             complement(1319..1996)
                     /locus_tag="ACGE4H_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFF8365575.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2033..3418)
                     /locus_tag="ACGE4H_002182"
     CDS             complement(2033..3418)
                     /locus_tag="ACGE4H_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFF8365576.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3628..3712)
                     /gene="ffs"
                     /locus_tag="ACGE4H_002183"
     ncRNA           complement(3628..3712)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGE4H_002183"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3735..4250)
                     /gene="tadA"
                     /locus_tag="ACGE4H_002184"
     CDS             complement(3735..4250)
                     /gene="tadA"
                     /locus_tag="ACGE4H_002184"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFF8365577.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4357..5214
                     /locus_tag="ACGE4H_002185"
     CDS             4357..5214
                     /locus_tag="ACGE4H_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365578.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5295..5630)
                     /locus_tag="ACGE4H_002186"
     CDS             complement(5295..5630)
                     /locus_tag="ACGE4H_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365579.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5697..7067)
                     /locus_tag="ACGE4H_002187"
     CDS             complement(5697..7067)
                     /locus_tag="ACGE4H_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFF8365580.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7145..>7658)
                     /locus_tag="ACGE4H_002188"
     CDS             complement(7145..>7658)
                     /locus_tag="ACGE4H_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8365581.1"
                     /translation="SKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYTHFKSI
                     SGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSL
                     FKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEY
                     LENILKEQSP"
BASE COUNT         2161 a         1636 c         1255 g         2606 t
ORIGIN      
        1 tcttctggaa gtacattgat gattggatca gtaacgggat cgaataacca gtcatcgttg
       61 ttgaaaaacg tttcatgaaa aaggataaaa aagcgatcaa atcctatcat catgaaaaaa
      121 ccgaaaacaa ctggcagtaa catgccgatt tgaaacggac gaatcaaccg ccaaagccga
      181 tcattctttt gcaaatattt gataaacaaa atactcggga tcaaagtgat gaaaaacaca
      241 gcataatcca ctaaaaacaa aattttcact tcataaaagt gatgggctcc gctagcagaa
      301 actgggaaat ctggcaagct gaggatggtt ttgaagggat tattgagata attcattagg
      361 tgatcaaaat ttttcagtaa cgtctcttga tctaaagaag tataatccaa aatttgcaaa
      421 tgtccgatat caaatacgta tagtggtcga aagttgatcg tcacaccaac tgccagactg
      481 atcagtgtca gaaataggct gaacaatcca gcatattcca tccactgcca tctcgaattt
      541 ttcatattag aagctccatt catccagtga ttgtttcaca taggtagctg ggataggcaa
      601 cccaggtaca tcttcttctt ttgtaaaacc tgataaaacg agcaaagtat ctatattatt
      661 gcgtatccca gcttggatat ctgtttcata gttatctcca accatgatta cttcttcttt
      721 ttgcagccct aatacttcaa cggctttatc cataatgatg gcttctggtt ttccgatgta
      781 gatcggcggc gtttgggttg ctgtctctac aaaagagatg actgaccctg ctccaggaag
      841 aaggccgcgt tcagtaggta tattcttatc tgggttcgtt ccaatgaaag tagcaccttt
      901 ttgaatagca agtgttgctt taactacttt ttcataggtc agataattat ctaagcctac
      961 cactacatag tcaggagcct cttcttccca tgtaaatccg gcagctaaaa tcaagtcaat
     1021 caatcctgct tctccgataa catagacttt tttccctttt ccatctgctt tcataaaatc
     1081 gattgttgcc aaagtagctg tgtagactgt ctctggagct acatgaatgt caaactcatc
     1141 tgctagacga ttagctactg tttctggtga cttagtcgtg ttattggtaa caaacaagaa
     1201 aggtaatttt cttttttgca attcttccac aaatcgttta ccggccggta tgggttgtgt
     1261 cccacgataa atcgttccgt ctaaatcaat taaatatcct ttatagtcca ttcatccact
     1321 actcttccct ttttctgatc gtaaatgctg tttttgtttt ttctacttgt ttaggttgtt
     1381 cttgtttttt tactttttca gaggtttttg ctgactctgt ttttcgattt ttaataaccg
     1441 gttttttgcg attagatgcg actggtcctt ttttctcatc gatatacgca cgattatggt
     1501 tttttttctt ttttcggcgc tgagttgtct tttctttttt accgccgatt ctctcgatga
     1561 caaaatacgc acaaccaaaa ttgcaatatt cgtaaagata gtcttccaag gaatcgattc
     1621 gctgatctgg atgcgcccgt cgattatccg cttggaaaaa tccttttagg cgtagttgtt
     1681 cataacccca atctcctacg atataatcat atcgggctag tacatcactg taacgttctc
     1741 caagcttttc tgcattaaat ccttcacgat aatcggacac aagccgataa tgtctttcac
     1801 ctatcaaaaa attactgtca tctaacagtg tcactagctc gcctttgact ttttctgctt
     1861 taggctcctc aacgatttct tccaaagcag ctgtcagttc ttcggtgact gttgtttcct
     1921 gttcagctga tgattttttc ttagcaattt ctgttttatg ctcaacgttc tgctgttcat
     1981 tttgttttgc agacatgcgt tcaccaccta tcttttttat tataccttgt ttttatttga
     2041 ttgggtagtg agctttcaag taatctccta ccacacttcg gataaattca gggaacaaaa
     2101 attcattttt tccagcaatc tcaatcgttg gaaaaaatgg aacaaacatc aaatgatcta
     2161 cggtagtaaa agtataattt ttattcagat cgattggctt gttcttccaa aagacttgat
     2221 ggttaataga atcatatgtg attccattat agaccatctg cccaaaaatc ttaccgcgaa
     2281 atcccattcc cttcatagga tagttacgta aaaagttacg atttttttct atttctaaaa
     2341 ctaagcggat cagatcattt ccacccaatg tgacatttaa caagtgcatc ggatgcggca
     2401 acgcagtgtg aagctgatcc tgattgaccc gcccttttgg aatttcagtt aaaaacagac
     2461 cactgtttaa tattgctgct tccgtctttc ctcgttcttt caccgcctca aaggctactt
     2521 ggataaacga atgctcattg aagatatcta gactcagaga aaatggcaaa tctgctactt
     2581 ctttttcttt taataattca tgcccacgtt ctagataact atttatttct tgttcatctt
     2641 ctgtaaaagc tgtcattgtt tcggtaggga tggctttggc tgaatgctta aggatttttt
     2701 tctcttcatc gatggttaaa tggacttcac cgacatactg cccgtacttt ccagcggctg
     2761 ctatttggac atcattgatt acgtgacctt ctctgaataa atgatgggtg tgcgatccta
     2821 aaataacatc aattgctggt atttcttttg caatttggaa atcatcctga atcccgagat
     2881 gactcatcaa aactaaaaca tccacttgtg aacgtaaatc ttcgactaat tctggcaaaa
     2941 tatcataagg attccgtatg tcccagccat tcggattata agtaagcgga aatggagcag
     3001 taaatgcaat tagtccgatt ttcgtctggt gtttcgttgt aatgatttta tatggtttcg
     3061 tccatgccgg tggctggagc gtatttttat cgaataagtt atccaggatg atatcaaact
     3121 cagcatgatc gtataaatga ttcaactctt ctttggaatt tcctacgcct tcattgtttc
     3181 ctatcgtcgc tgcatcataa ccaacttcat tcatcagcaa gatattttcc tgtccattcg
     3241 ttgcttctga caatggatgc caacgatcca caaaatcacc tagatctact gcaatcgttg
     3301 tcgttttttc tttcttttcg ttttctcgtt tacgttgatc caaaaacctt ctgatccttg
     3361 gccaattttc taaatgagaa tgcagatcat tcgtatgtaa aatcactatt ttttccatgt
     3421 accctctcgc ctctctttat ctttatcata ccataggcag ctttttcttc cattgaaaca
     3481 agctctattt cagagttttt cctgccttga agcaaattgt ataagtacaa tattcgataa
     3541 atcagtctgg ctaaaaaatc aggaacattc atttttacag tactataaaa ataaaaaaaa
     3601 cagacccgaa agtctgttgt cgtttcatta taatcagaca catggcggaa cctgcttagt
     3661 gctgcttcct tccagacctg acacgcttca caagcccacc attgtcctag cctttcggca
     3721 aagaatagta taccttattt ttcattcgtt gaccagtctt tttttaattt cttttttcgc
     3781 tggcggagct ttcggaaaaa gtcagtcaaa atcatcccac attcgtcttc taaaatgcca
     3841 ccttctacat atgcttggtg gttaaaccgc tcgtcctcca aaagattcat caaggtaccg
     3901 gctgttccgc cttttggatc atacgcgccg aaataaactt ctgctacacg agacaaaatc
     3961 atcgcgccgc tgcacatagg acaaggctct aaggtaacaa aaagtgcagc atcttccaag
     4021 cgccagcttc ccactttttc acaggcttcc ttgattgcaa gcatttctgc atgtgtagta
     4081 gcatcttgcg tggtttctct aaggttatat cctcttccaa tcacttttcc atccaataca
     4141 accactgctc caatcggaac ttcatgaagc atttctgctt tctttgcttc tgccaatgca
     4201 aggcgcatcc atttttcttt ttctactgca tcaagatttg tatttaccaa ttacccttcc
     4261 ctgcctttgt tttttcttgt tttactttag catatgataa aaaggctgta actaactttt
     4321 ttttctatgg taaaataaat acaagaggtg aacaagatga aaatggataa attacttgct
     4381 ctttacccac aagcacagaa aaaaaactct ccagcatcag aagcggatat cctttctttt
     4441 gcaattgatg atgcttttat ctggatcaaa caagattcat tatctcaaca agaaaccagc
     4501 cttttgaaag cgttattccc tgtaatcaac gatcaaaaaa aacacccatg gtatcactat
     4561 ctttttaaag atgcttcttg tgttcaagaa aaaagctttc gtttgatcca gcttcacgtc
     4621 gaatcaaaag gagaattctt aaaaaaagaa tggcaagata ctattttgga attatttcgc
     4681 tattctgctg atttcttttt ttatacagac acagatgcgc tcttagtcga acaaataaac
     4741 cgtgattatc ttgatgctac tgaaatcaac ggtatttttc tatctttaga tgctgatttt
     4801 gatacgacaa cgtctgtatt cgttggttct ttccattcac ctggcaccga cctcgtccct
     4861 ttgtttgctg aagaaagaaa aattttttta acagagcaaa acaatcttta tactcacagc
     4921 cgtacttttt ctatgcaaga tgtggcttta cattactata caaaagatac tgtaaaagat
     4981 agtaagttga tcaaaagtta catagaactg atcaaacaaa atgaaatgga agtgatcatt
     5041 caagaattat ggaatgaatt aggcaatgtc agctctgctg caaaaacact gtttcttcat
     5101 cgtaatactc taaaatatcg aatcgaaaaa tttcaagaac agtctggttt caatctaaaa
     5161 aaagccaacg atttactgtt ttgctatctt ttattgcttc atgaacataa ttaaagatag
     5221 attaaagggg ttgtgacaat aattttgtca caacccctta tttcgaatga atagtgttca
     5281 aaagctagtt tctcttacat cattttttca acaaaatgca ttagccggtc ttttacatct
     5341 tcggttgaat ctttgattga atcaccataa acagagattt tcttttttcc tgatttgatt
     5401 tttgctaaca ttcccatcaa agaatccaag tcactatctg aaagaccatc tacgatttct
     5461 agtaattttt catttccatc aaatttgtca tagacgaatt tttttacttt cgtccgagta
     5521 gtcatatgat ggatcttttc cattactttt cccgatacaa cgactgctgt cgctacactg
     5581 gcagcggctg ctacgcttaa tccgattgtt acttttgttg atgttttcat aagtatcagc
     5641 tcctttttat ctatctccat catagcatag acaaaaaaat aaacgaacta atccactcag
     5701 tgaaatctca atcctttagg gaaaaagttc tttactgttc cttttacttg tttcccaaat
     5761 ccgacaggta tcttatctaa tactagcaaa acccatcctt gattcccatc acgttgaaat
     5821 gtttctcctg ctgtgtaaga ttgccattcc ttttgagtga tcggcaagca aggtatattc
     5881 tctatctttt ttgtagcaag cgctaatgca tagctaggtt caaatcgatt ctttttgaaa
     5941 tctcctaaat gcaaacctgt acgtacgact tttaaaccat cgagagaagg ggctaactca
     6001 ggaacttccc aaagatggtc gttgaacact aaaagtcttc cagtagcttc ataatgaaaa
     6061 tcatttgaaa actccgtcca gagtttttcc tgctcttttg tcatttgcac tttacttttt
     6121 tttcgtgtct tcttttcttt atgcatctga ttttgtccat gaaaagtcag ctttgcaaca
     6181 aaatgtcctt caccctgatc tttatgaggc catatacgta ttgttttttc caaaccagct
     6241 acgcttcccc attcagaacg tcctgaacta acggattgag tcaaaggaat ctcctcaatc
     6301 gttactggat aattttctac aagccagcta atgatctctt cattttcttc tggtgcaaac
     6361 gtacaagtcg aataaatcag ttgtccttta ttttttagca tttttattgc tgaagacaaa
     6421 atctcttgct gacgtttttg acagtatagc ggagattctt ctgtccactc ttttattgca
     6481 tttggatctt ttcggaacat tccttctcct gaacaaggag catcaaccac gatccgatca
     6541 aaaaagcccg aaaaatgagg gacaagttct gcgggtgcat gatttgtcac tattgcgttc
     6601 gaaactcccc atcgttctat attttctgat aaaatcttcg cacgtttcgg aaagatctca
     6661 ttcgtcacta ataacccttt ccccttcatt tgagcagcta attgggttga tttaccaccg
     6721 ggagccgcac acaaatccaa aactttttca ccgggttttg ctgcagcagc tgttccaacg
     6781 atcattgcac ttggttcttg gctatattca tacccagctt gatggaggaa agactttcca
     6841 ttgacagtgc caagaaatcc ctcattgctg tagggagcgg gttgtaattc ttcgctatgg
     6901 taagtttgga ccatgtccaa tcctgccggt tttagtggat tccaccgaaa tccttttttc
     6961 acgcttcctt gttcaagcgc agaaaaaaaa tcggatgctt cttcgcctaa aagaagacga
     7021 tattttttga taaattgctg tggtaacgtt gtcgcttcct ccttcattcc aatgtttcgt
     7081 tagacatttt acaatctttt ttacgaaaaa taaaggtggc tgatctactt tttcatgttg
     7141 ctgcctaagg tgactgttcc tttaagatat tttccaagta ttcatccgtc acctcgtttt
     7201 tatcccaaac agcgttcatc atttcaagaa aatcttgaga agaatcatct gagcgcaaac
     7261 ttcccaatgc attaagccat accaattcaa gcgacttttc ttctttgatc ttcgtgtgac
     7321 ctttgacata tgagaaaaat ggccgcttaa atagcgaatc catgtctaaa tcagattttg
     7381 caatgatttc acgaatactt tcatggatag aaaaaaaatc tttcaataag tagacaatgc
     7441 ttaaaacatc atcggaactg cattcagcta agtagttttc aaaataaaca gtgatttctt
     7501 ctaatatctt ctcttttaac ccgctgattg atttgaaatg tgtataaatc gggtatgtag
     7561 aacaaccgca gaactctgct aactctcttg cagtaattga cttcattcca tattcttcca
     7621 cgtataccat acttttgaac agcaaatatt ctttacta
//
LOCUS       DAXWLB010000131         7546 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXWLB010000131 DAXWLB010000000
VERSION     DAXWLB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.133"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE4H_002189"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACGE4H_002189"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFF8365582.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACGE4H_002190"
     CDS             complement(2032..3285)
                     /locus_tag="ACGE4H_002190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFF8365583.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACGE4H_002191"
     CDS             complement(3291..4214)
                     /locus_tag="ACGE4H_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFF8365584.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACGE4H_002192"
     CDS             complement(4435..6927)
                     /locus_tag="ACGE4H_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFF8365585.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACGE4H_002193"
     CDS             complement(6934..7401)
                     /locus_tag="ACGE4H_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFF8365586.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2078 a         1643 c         1212 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaag
//
LOCUS       DAXWLB010000132         7492 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXWLB010000132 DAXWLB010000000
VERSION     DAXWLB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.134"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..243
                     /locus_tag="ACGE4H_002194"
     CDS             <1..243
                     /locus_tag="ACGE4H_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFF8365587.1"
                     /translation="CTSEACDFKLWTTIAKKKLTATQLKEIIQNSRTSQPVKGLKGQK
                     GSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            259..408
                     /locus_tag="ACGE4H_002195"
     CDS             259..408
                     /locus_tag="ACGE4H_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365588.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            482..1075
                     /locus_tag="ACGE4H_002196"
     CDS             482..1075
                     /locus_tag="ACGE4H_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8365589.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1091..1990
                     /locus_tag="ACGE4H_002197"
     CDS             1091..1990
                     /locus_tag="ACGE4H_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8365590.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            1993..2478
                     /gene="ssb"
                     /locus_tag="ACGE4H_002198"
     CDS             1993..2478
                     /gene="ssb"
                     /locus_tag="ACGE4H_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8365591.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2844..3104
                     /locus_tag="ACGE4H_002199"
     CDS             2844..3104
                     /locus_tag="ACGE4H_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365592.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3545..3751
                     /locus_tag="ACGE4H_002200"
     CDS             3545..3751
                     /locus_tag="ACGE4H_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8365593.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3751..4002
                     /locus_tag="ACGE4H_002201"
     CDS             3751..4002
                     /locus_tag="ACGE4H_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365594.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            4017..4454
                     /locus_tag="ACGE4H_002202"
     CDS             4017..4454
                     /locus_tag="ACGE4H_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365595.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            4447..5154
                     /locus_tag="ACGE4H_002203"
     CDS             4447..5154
                     /locus_tag="ACGE4H_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365596.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            5319..5519
                     /locus_tag="ACGE4H_002204"
     CDS             5319..5519
                     /locus_tag="ACGE4H_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365597.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            5535..5714
                     /locus_tag="ACGE4H_002205"
     CDS             5535..5714
                     /locus_tag="ACGE4H_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365598.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            6055..6318
                     /locus_tag="ACGE4H_002206"
     CDS             6055..6318
                     /locus_tag="ACGE4H_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFF8365599.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            6312..6662
                     /locus_tag="ACGE4H_002207"
     CDS             6312..6662
                     /locus_tag="ACGE4H_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8365600.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(6686..7300)
                     /locus_tag="ACGE4H_002208"
     CDS             complement(6686..7300)
                     /locus_tag="ACGE4H_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8365601.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         2691 a         1222 c         1456 g         2123 t
ORIGIN      
        1 tgtacgtctg aagcctgtga tttcaaacta tggacaacga ttgccaaaaa gaaacttaca
       61 gcgacacagc tgaaagagat cattcaaaat agccgaacaa gccaaccagt gaaagggtta
      121 aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc
      181 tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cactacaaaa
      241 taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa
      301 aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca gcaaacgaag actgcagcaa
      361 ttgaagaacc agcagcggat tcaaaaagaa atcgaattag atcgataaat tcctttcttg
      421 ttaatataaa actaacatgt tatagttgtg ttagttttat attaacttta ggggaagagt
      481 aatgaaagac aaaatccaat gtatttttga taaacataat ggcaacctat ccttgaagga
      541 tgctagaagt gaaggattat cgcctatgac gttagatcga ttagccgaaa aggataaaat
      601 agaaaaggta gcaccaggtt tctatacctt aaaggatgcg ttcgtggatg aattgtatct
      661 cgctcagagt atctattcca gagggatttt ttctcatgag actgcgctcg atctttttca
      721 agtaggaaca tatatcccta aaaagatcaa tatgatgttt cctaaaggat acaatgtatc
      781 aaaggagaaa ttagatcagt atgccattca accgcattac actaataaag aaaattttga
      841 cctgggagtc acagaaacag aaagctttta cggcaataaa attttagtct atgatttaga
      901 gcgaacgtta tgtgacatgt ggaatccaag atacaaagca agcgtagagg taaaacaaga
      961 agcattaaaa gaatatatga caagtaccag tagaaataca aataagctaa gaagatacat
     1021 ggatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     1081 gtaaagatga atgacaccga cacaatttaa agagaaaaca agaaatttcg cagcagagaa
     1141 acatttaaac ccacaaattg tccaaagata ttatatgatg gaaaaattat tagaatgtat
     1201 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga ttggatcaaa
     1261 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     1321 aacaaaagaa aagttagaaa ctgttttttc tgaattaaca gctaatccta caaaagatgg
     1381 aattcgtttt gaattaaagg gattaacaga gacgagggaa gccgattatt atccaggttt
     1441 tcaagcaaag tttattgctt atcttgaaaa tgcacgtgta ccttttaaat tagatattac
     1501 ttcaggtgat tctattattc cagcagcatt gatgtatgaa cacaaattaa tgtttgaaga
     1561 taaaacgatt catattccag cttatcctac ggaacaaatt ctagctgaaa aattacatgc
     1621 cacgtttagt tttggaaaag ataattcgcg catgaaagat ttttatgacc tgtatatgat
     1681 tccgaaatta gaacgtatcg atgagaaaaa tttgtataag agtgttcaag gaacattcaa
     1741 ggaaagaaat aatcaatcgg tgtttaagca tttgtatgat caggaaatgc ctcaattggt
     1801 gaaagatgag ggcttgagag aaaagtggaa aagataccaa caagaaaact attttgccaa
     1861 agacatttct ttcgaatcta cactaacttc cgttgattcc ctgatgcaat caattgtaga
     1921 gcaagagaag gcagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     1981 ggagagataa agatgatcaa taatgtaact ttagtagggc gattaactaa agatccagat
     2041 ttacgttata ccgcaagtgg cacagcagtg gcgacttttt ccctagccgt taatcgtaat
     2101 ttcacgaatc aaaacggcaa tcgagaagct gattttatca attgcgtgat ttggcgcaaa
     2161 ccagcagaaa cgatggcgac attagctaaa aaaggcagct tgatcggcgt tgtcggacga
     2221 attcaaacac ggacttatga caaccagcaa ggacaacgtg tgtatgtgac agaagttgtg
     2281 gccgataact tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     2341 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     2401 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     2461 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     2521 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     2581 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     2641 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     2701 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     2761 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     2821 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     2881 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     2941 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     3001 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     3061 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     3121 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     3181 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     3241 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     3301 agacaaaata gacaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     3361 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     3421 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgatttatt ctggccaaca
     3481 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     3541 aaacatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     3601 tcatgcacca catactgttg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     3661 tcgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattacat
     3721 tttgattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta
     3781 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
     3841 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
     3901 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
     3961 acttatgaaa tcgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaatatgg
     4021 ctcaaaatta ctatgacgaa tttgtcaaat tacctttgga taaaatggca caaaaaatgg
     4081 aggatatgac gttcctatac catgaaacac gtgtacccaa aaaacattac aaggaaaaat
     4141 tgtcagtcgc agtggaagaa atgattgaat caggtgtcga aatgaatctc attgcgacgt
     4201 actatcgaac actggaagaa ctgaaaaaac aaaatggaaa atggtttttc caagccttac
     4261 tatgcttgga agtaggtgtc aaaccaagca ctatcaaacc ttccgaatat caagccttag
     4321 aactgactta tgcaaaattc gcacagatta agaaagctaa agcaatggct agcgactggc
     4381 tagatacttt tgaaagtatc gaaaaaaatg gtgcatttta tacgattgaa tcggaggaga
     4441 aagataatga ataaggtggt ttatcagcta acaacagaag atgtttcgca atttttaaca
     4501 agcaaagaac acttgaatga agtaatcaga cggattcaaa aagactttag ttgtatggaa
     4561 gaaatgaagt actttattca aagagtggaa gaacagttgt atatggaaag cgaaacaagt
     4621 caaacgaagg tcacagtcag ccataatcat ttagacgtgt ataaacgtcg tcctgaaacg
     4681 gtaaaaaaag gaacagagct tctacgacta gaaacagaaa aatacatcat gaatgtgtac
     4741 ctagaaagga gtgagctggt cgaaaacttt gtcagctaca cgatttgtag gaaacactat
     4801 tactcagaat cagaatatta ttcaattttc gagagcatgg aattaatgga agaagaaatg
     4861 gaagagctaa acagcaaaaa tgcgacggta gacgattatt accttgtatt tactaggttt
     4921 ttgacgtgtg ggacggaatc tatcgtgtta atgggaatgg attatgaact cgctgtaccc
     4981 gaagcatttt tgagggaata tgtagaagaa gtagataacc caggcgacga ctggtacacg
     5041 actttaactg attttttaga cacctacact tacgacgata cagaagaaat ccaaaatgaa
     5101 cttgaccgta aacgaatgcc atatcaacga tttgaaagga aaaaaggtaa ataaccattt
     5161 ataccgaaaa gtgaggatgt gtgaaataag aacaaaaaac cttgaaatta agggaatggt
     5221 tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg
     5281 gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc attcctctat
     5341 tgttcgtact gtttgttggc gagatactta tgcgtcatgc tgcagctgta catttaaact
     5401 tcgtagtatt cttaggaata ctggtatttt gtttgatctt ttggcgattt gtattagccc
     5461 ttgtgagtct actattcaag atgattgcag ccatattttt catagggtta tttcgatgaa
     5521 aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg
     5581 aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga
     5641 ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag
     5701 cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt
     5761 cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa taactatgaa
     5821 atctttaaag tatccagaag agattatttt tagatcaatt tttttcttac ctgattctaa
     5881 agattttgtt ttcttaaaca tattatgatt tcctttcgac tattttcata taaatgttaa
     5941 catagtttat ggattcatgg aatgttaatc taatttaata gaaaaggata attaagtata
     6001 tacgtttttt cttatgtatg ttatactatg tatatacaga aagaggtgtc tactatggaa
     6061 atgttaccta taaaaaaatg gggaaatagt aatggcctta gattgccaaa gtatattatg
     6121 gaatatcttg gaattcatac agaagacaaa gtgaagataa tacaagaaga agtaaacggt
     6181 cagaagcggc taattatcga agcggctaat atagaaaatg aattaacaat tgaacagtta
     6241 tttgaaaatt acactgatga aagaaatcat gtgacgattc aaaatttagg tgaagcagta
     6301 ggtaatgaaa aatggtgaga aaacctagac aaggcgatat tctattattg aatacagcac
     6361 ctcgatctgg acacgaacag acaggtaaaa gaccttatat tgttttaagt catgatatta
     6421 ttgcggatta tagtaacgtt gttacagttg ctccaatttc tagtacaact agaaactatc
     6481 cgctatatgt aaatataaac tcagaatatg aaatgaagac aacaggaaaa gtattattag
     6541 atcaactaac gacaattgat tatgaagcca gagagtgtat ttttttggaa aaagcgcatg
     6601 atagtttagt tgaggagtta ttgatgaaag ttagaacagt ttttcaaaaa gtaagcaaat
     6661 aaaatagaaa cagcagcatg atcaactatt cgtgattgac catgctgtct ttttttattc
     6721 tatatacggt ttgtctcgta acattgtagt cttttgcaat cttagcgatc gcaacacctt
     6781 tttttagatc ctcaacgatg tttttataaa caagacgacg ttggggatct tttgcattgg
     6841 cactgtatag cttaggacgt cctttataaa caccgttcgc ttttgcaatc ttaatgcctt
     6901 gtgcctgacg tcgtttagat tcggtacgtt cttgttcagc gatcatggcc aaaatttgaa
     6961 tgatcaaatc tttaatgaat ttatccagga gaggattgcc aattgcttcg gccatgatcg
     7021 gtagacttgt gatgatcagc tgcacatttt tcttcttcaa gtaattgact gaagcgatga
     7081 tctcgtcgta atttcttcca agtcgatcaa tagcttccac aataaaaata tcttgatctc
     7141 ggacgaaatc cagtgcttct tgaaaaacaa gtcgatgagt aatcgttgcg ccagattgtt
     7201 tctcaatgaa aattctctct gcaccgaatt ttttcatttc ttcaatctgc cgttgttcat
     7261 tttgatcgat ggatgagaca cgtatgtatg caactctcaa aatttttacc tactttctgc
     7321 aactatttaa aaaacaccct aatcatacac gagaaaacca gatttttcaa tgtcgcggtc
     7381 gtgtctagtt agggtatacc ttatatagac aaaaactaat gatggaggaa atagtatgtg
     7441 tcacttttct ttttaaatgg caatattttt tgtaaataaa tgtcaatcaa ga
//
LOCUS       DAXWLB010000133         7476 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXWLB010000133 DAXWLB010000000
VERSION     DAXWLB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.136"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            135..998
                     /locus_tag="ACGE4H_002209"
     CDS             135..998
                     /locus_tag="ACGE4H_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFF8365602.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1127..2749)
                     /locus_tag="ACGE4H_002210"
     CDS             complement(1127..2749)
                     /locus_tag="ACGE4H_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFF8365603.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2998..3519
                     /locus_tag="ACGE4H_002211"
     CDS             2998..3519
                     /locus_tag="ACGE4H_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8365604.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3705..5435)
                     /gene="spxB"
                     /locus_tag="ACGE4H_002212"
     CDS             complement(3705..5435)
                     /gene="spxB"
                     /locus_tag="ACGE4H_002212"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFF8365605.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5600..6418
                     /locus_tag="ACGE4H_002213"
     CDS             5600..6418
                     /locus_tag="ACGE4H_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFF8365606.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6521..6718)
                     /locus_tag="ACGE4H_002214"
     CDS             complement(6521..6718)
                     /locus_tag="ACGE4H_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365607.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6887..7465
                     /locus_tag="ACGE4H_002215"
     CDS             6887..7465
                     /locus_tag="ACGE4H_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8365608.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2211 a         1363 c         1490 g         2412 t
ORIGIN      
        1 agctgaccac ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa taaaccggtt
       61 aaagtgtgtc tagcgcaaga gatttgctat aatagacgat aagggtaaaa tgtaacgaga
      121 aggggttgaa aaatgtgtat caaattgtaa cagattcttg ttgtgatctg ccataccatg
      181 tattagcaaa agaaaaagtt gattttttat caatgaatct ccaattgaat gatcaagagt
      241 tagtagatga cctcggaaag acattcaatt atgaccgcta catgaatcag ctgaaaaatg
      301 gtgcgatgcc tacgacatca caaatcaatg ttggccgata tttagaattt ttccgttctt
      361 atgcggaaaa acagattcct gttttatatg tggctttttc cagtggatta agcggctctt
      421 acagtagtgc gcttcaagcg gttgaattgc tgaaggaaga atatgaagac cctgagatct
      481 atgtttttga tacaaaagct gcaagtattg gggaagggct tttagtattg gaagctgtaa
      541 gattgaaaaa agaaggtaaa agcatcgaag aaaccctaga gtggctggaa agtaattatt
      601 tgaaagtcca ttcatgggta acagtagacg atctgaaata tctagaacga ggcggtagga
      661 tttccaaagc aagtgctact cttggcagtc tattgagtgt aaagcctatt attacggtgg
      721 ataaagaagg aaggctgacg aatgttgaca aagttcgagg aagaaacaag tcgatccaga
      781 caatcgtcaa tgagacggta aaaacaatcg ttgcaccact tgatcaaatc gtttatgtgg
      841 cttacgctgg ggatctagag agcgcggaga aggcaaaacg gcttttggaa gagcagataa
      901 agatgaaaga tgtgaagctt tatcccttag gaccaacgat cgccagtcac acaggctatg
      961 gctgtatcgc gattttttcg atgggaatca gtcgataaaa gcagtaactc attctgtaaa
     1021 aaagcccaga aattgattgt aacaaaaaca gcgactctta aaagatagat gagcatccta
     1081 tctcttagag gtcgctgttt ttttattttt taagctaaat aggccattat gcccataact
     1141 cgttcaattg tttttgaata tctgcatttt ccaaaaattc atcatatgtc gtatcagctc
     1201 gatcgatcac acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt tggataaatt
     1261 gatggtcatg tgacgcaaat aagattgagc cagtaaatgc catcaagccg tcattcaatg
     1321 ctgtgattga ttctaagtct aaatggttcg ttggatcatc taatacaagt acgttagctt
     1381 tcgaaagcat catttttgaa agcatgcaac ggactttctc tccaccagat aaaacattca
     1441 ctggtttcaa tacttcttca ccagaaaaca acattcttcc taagaagctt cttaagaagg
     1501 tgttatcatc ttcttcttta cttgcatatt gacgcaacca gtctaaaatc gttagatcag
     1561 tatcgaattc tttcgttgta tcctttggta aataagaatt acttgttgtc acaccccaac
     1621 gtactgttcc agtatctggc gtaatttcac ccatgataac tttgaatagc gttgttgttg
     1681 ttatatcatt ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta aaagatatat
     1741 catctaaaat tttctttcca tcaatcgtta cacttacatt ttctacctgc aataagtcgt
     1801 ttccaatctc acgttcaggt ttgaatccaa cgaatggata acgtctagaa gaaggttgga
     1861 tatcgtctaa agtaattttt tcaagcattt ttttacgtga agttgcttgt tttgattttg
     1921 atgcattcgc actaaagcgg gcaataaatt cttggagttc tttgatttgt tcttcttttt
     1981 tggcatttgc gtttgcttga agtttagccg ctagttgact agattccaac caaaaatcat
     2041 aatttcctac ataaagtttg attttaccaa aatctagatc cgccatatgt gtacatactt
     2101 tgttcaaaaa gtgacggtca tgggatacaa cgatcacagt attttcaaaa ttgatcaaaa
     2161 attcttccag ccaattgatc gattggatat ccagaccgtt tgttggttcg tctagcaaaa
     2221 gaacatcagg tttaccaaat agtgtttgtg ctaataacac ttttaccttt tgtccaccag
     2281 ttaattcact catttgctga tggtgcaatt cttcagggat gttcagacct tgcagtaaaa
     2341 cagctgcttc tggttcagct tcccatccat taagctcagc aaattctcct tctaattcag
     2401 ctgctcgaat accatcttcg tcagtgaaat cctctttcat gtaaatcgca tctttttctt
     2461 tcagcacttc atacagacgc ttgtgtccca tgatgactgt ttcaagtaca gtatagtctt
     2521 catagtcgta atggttctgt ttcaatacgg ccatccgttc atctggtccc atagtaacgg
     2581 aacctgtgga aggctgcaga tcccctgata acactttaag gaacgttgat ttacctgcac
     2641 catttgcacc aatcaagcca tagcagtttc cgggggtaaa tttaatattc acatcatcaa
     2701 atagtttgcg gtctgaaaag tgcaaactca catcatttac agtaatcaat ttttttcctc
     2761 acttttatca ttattagttt tgacaagtct tatagaatta tataggggaa aggtaaaaga
     2821 ttcaaggcaa tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa aataaaaata
     2881 aaagcggagc gaaaatagct actataggat ttattagttt gcaacagcat aatttttgac
     2941 gttatttata agagggagta ctataaaact gtaaaagaaa tgaaagaagg attgaaaatg
     3001 gatgaaaaaa gaaccatcga ctggggctca ttgattttgg gaattttatt tgtacttgtc
     3061 tcattgttat catttcaaga ccctgtcgga aatttagttg caattgtagt agtctttgcg
     3121 atttttgcct ttattaaagg gatatttgaa ctgtttgtac ggaacagatt aaaagagcta
     3181 acaggatata aaggaaagat gcccttaata atcgggatca tcgatattct agttggcgtc
     3241 tttttcttat tcaatattgg tgcaggagtc gcagcattgc cttttgtttt tgcagtatgg
     3301 tttattgctg attcagtttt agcgttattt actgctgact tggcaagagg agtaagtgaa
     3361 ggatattatt ggtttacgat catcgtcaat attttaggaa ttttattagg aattttctta
     3421 ttgtttaatc caatttcttc tgcgttgaca ttaagctttt tagttggatt ctattttatg
     3481 ttatttggta tcacacatat cgtctatgca tttagataag taaaatcaaa caaataaaaa
     3541 agccggcttc cgattacagt tttactgttg taattggaag ccggcttttt tatcatggac
     3601 aaatatgcac gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat gtgagaagct
     3661 atatacacaa attttatctt aatactcgaa gcatagcgca gttgtcacaa cctcttttca
     3721 tataattcat tcaatactgg caaatcatgg acttcatatt tttctttgaa tgcttggatt
     3781 tcttcttcag aatattttct tggatctaaa tgcagatctt ctactggcaa tgggcgcttg
     3841 ttattgatct tcacatcgat tactacagga cgatctgatt ttgcagctgc atcaaaggca
     3901 aatcgaagtt gcgagtattc tgtgactgta aagctatttg ccccgagcgc ttctccaact
     3961 ttaccgaaat ctgcatcttc taaaaataca ccaaatttct tttgttccgt atcttcttgt
     4021 tctgcttcga tgaaaccaaa agaatcattc gaaaagacaa cattaataat tggaagctga
     4081 tatttgactt gtgtaatgat atcttgcatg accatagcaa acccgccatc accacttaag
     4141 gtaaatactt gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg cacaccatta
     4201 cccatagttg cgaaccatcc agatgtcgta tgtttttgtt taccgttcat attcaataaa
     4261 cgaatagaat ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc gtcttcagca
     4321 atacgattga tttctttata gacagcctct ggacgaatcg gttcttcctc tcgatcttca
     4381 aaagatttca accaatttac ccatttttct ttattttctt gatttgctag gtaccagcta
     4441 tcagcagggc gagcatcccc taactcgaca agtctttgca aagctgtgtt tgcatcaccg
     4501 aggatcgaca aagatgtttc atggcgtcgt ccaaattttg acgcatcgat atcgatttgg
     4561 ataaattcag cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct accgacaaat
     4621 aagatcagat cggcttctgc caatgcttca tttccgggtt tagtagcgac acgtccagca
     4681 aaaccaagaa agttttcata atcatcagga ataattcctt ttgctagaac ggaagcgaca
     4741 acaggaactg aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt tcgcgttcct
     4801 tgaccaacat aaagaactgg tttttttgct ttttcaagat aaggcaaagc agctagcaaa
     4861 tctttttctt ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga gaaaggttgt
     4921 tcgtcaattt cttcaaaacc aagatcaaca gggatagtca cgatagccac gcctttatgc
     4981 tcataggcgg ctttaatggc ttcatctact acatgcggaa gactttgtgg tgtcatgact
     5041 gtacgattgt atacgctaac atctgcgaaa ataggatttt catttagttc ctggaaagag
     5101 ttatagttca tggatgtaga agcaacttgt cctaagaacg ctactagagg cacatggtcc
     5161 atctgtgcat catacaaacc attgatcaaa tgtgtcgcac caggtcctgc agagccgaag
     5221 acagcgccga ctttccccgt taattttgca tctgcagcgg ctgcgagtgc gccgacttct
     5281 tcatgacgaa cttgaatgta gttgatttcg gttttttctt gatatagcgc atccataatt
     5341 gaattgaaag atcctccggg aatgccgtaa atatggtcga taccccagct ttcaagaact
     5401 tttaccattg cgattcctgc attgattttt gacatggtgt ttcactcctt tagcaatgat
     5461 gatacataaa ttttttgtat ctagagtata gaacttttta tcgaaaatcc caactaattc
     5521 gtcttttcac aaaaatgtag tcatgttttt gggaatatgc tagagtagta catagataga
     5581 ttcaagagag gggaatggca tggttgaatg gctattgaga cttgaacctt ggcagcaagc
     5641 acttgctggg acagctttta cttattttat gacagctctt ggtgccgggt tggttttttt
     5701 ctttaaagaa atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat
     5761 gattgctgca agtttttggt ctttgttaga tccggctatt acaaaagcag aagaaaatgg
     5821 agatattgct tggctagttg taagtattgg gtttggttta ggcggtgtgt ttttatatat
     5881 ggcagataaa acactgcccc atatgcattt tggaccacaa catgaagcag aaggattgcc
     5941 tacccattta aaacggacga tcttgcttgt tttttctatt acattgcata acattcctga
     6001 aggtttagcc gtgggggtag cattcggtgc agcagcaact gcggataatc cgacagccgc
     6061 tatattagcc gctgtttcgg tggcgctagg tattggtatc caaaacttcc cagaaggagc
     6121 agctgtatca atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg
     6181 acaagcttct ggaatcgttg aaccaattgc tggagtgatt ggagcgctac tagtaacgaa
     6241 agtggagctg ctgcttcctt atgcacttgc ttttgccgca ggagcaatga tttatgtcgt
     6301 ggtggaagaa ttgatccctg aagcacagca gacattgtcc agcaaacgtc attttgcagt
     6361 ttttggcgtc atgagtggat ttatcattat gatgattttg gatgtggcat taggataaat
     6421 aaaaaaaata tatatgatat atcaaatagg gccgcgaact tatatcgcga ccctattttt
     6481 ttagtaagtt atatcataat gaagtgaatg gtatcataca ttaataatag ttttttctat
     6541 gtgtatttcc tttgatccag cgaacaagcc agatgataag tgcgattggg attagaactt
     6601 tgaccgcaaa ccataaaagc ccgcttagga cactgacaaa aagactgatg acgaagctag
     6661 ctgcaattag cactaaaaga accatgaaga tatttaagaa catatttttt tgacgcatac
     6721 aaaaccctcc tcttgttcat tctttactat agaaaaagta taacagggat tgtttaaaaa
     6781 cacctccaac tgaagactga tttttacttc atctacttaa atgatcaaac aattgctttt
     6841 tctctggttt atcatctaaa atagagcgga aaaggaggag aatatcatgc ctaaaaatcg
     6901 tcttgaagca tttacagatg ctgtaatcgc gattatcatg actattttag ttttagaact
     6961 tcatgaacct aacggagaca cgtgggaggc gctcgctgca ttaggttacc gattttttat
     7021 ctatattgtc agttttatca ttttagcgat ttattggaac aatcatcatc atttatttca
     7081 agttgtacat aagataaatg gacgggtctt atgggcgaac agtttctttg tttttgcgct
     7141 ttcgcttttt ccgtttgcaa cttcatggat cagtgagtac atcaatagcc tggctccaga
     7201 aatgacttat ggttttgtta ccttgaccgc taatattgcg tattatatct tggcaaaaga
     7261 acttgtcaaa gcaaataaag gccacgagaa agcattgaag gcgatgttga ataattacaa
     7321 gaaatcgtat ctatcgattg gtttgaatgt gttaggaatt gtcttagggt atttagtagc
     7381 gccagtagcc gtgctgatcg caaacatcgt gattctagct gcttggatca tcccaagtcg
     7441 taccatcgaa tcacaatatc ggtaaaatga tccgtg
//
LOCUS       DAXWLB010000134         7429 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXWLB010000134 DAXWLB010000000
VERSION     DAXWLB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.137"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            275..922
                     /locus_tag="ACGE4H_002216"
     CDS             275..922
                     /locus_tag="ACGE4H_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFF8365609.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    1051..1267
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1315..3960
                     /locus_tag="ACGE4H_002217"
     CDS             1315..3960
                     /locus_tag="ACGE4H_002217"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFF8365610.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4270..5583
                     /locus_tag="ACGE4H_002218"
     CDS             4270..5583
                     /locus_tag="ACGE4H_002218"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFF8365611.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5573..6226
                     /locus_tag="ACGE4H_002219"
     CDS             5573..6226
                     /locus_tag="ACGE4H_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8365612.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6281..6973
                     /gene="radC"
                     /locus_tag="ACGE4H_002220"
     CDS             6281..6973
                     /gene="radC"
                     /locus_tag="ACGE4H_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFF8365613.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7123..7323
                     /locus_tag="ACGE4H_002221"
     CDS             7123..7323
                     /locus_tag="ACGE4H_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8365614.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2448 a         1262 c         1627 g         2092 t
ORIGIN      
        1 ttttgtgaaa tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag
       61 gttgcgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
      121 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt
      181 gtctcacacc cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac
      241 taaatatgtg aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc
      301 aaaagcaact gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc
      361 aggtataagt agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac
      421 gatccgcaga gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt
      481 cccttttctt atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc
      541 attagtagga tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga
      601 aaatctaaat atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga
      661 tggagaaatg atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac
      721 agtagcaatc tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa
      781 aggaaatgta accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt
      841 caatgttcaa tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa
      901 ttactcagaa gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg
      961 tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt
     1021 tgactaaaaa aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg
     1081 cacattcatt cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag
     1141 gtagcttggg agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc
     1201 acttgagagg caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt
     1261 cctagaatac gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg
     1321 gaagaaaata acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag
     1381 cattggttag aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca
     1441 atcgtaatcc cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg
     1501 accttgcaag atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg
     1561 cctggtatgg accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag
     1621 caagggattt ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg
     1681 aaagaagagt atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat
     1741 tacagtaggg aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt
     1801 gttaccttat acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct
     1861 caagcacgaa cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt
     1921 tatcatatga gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt
     1981 ccagaaacga tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa
     2041 gcgctcatcg gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca
     2101 gatgaatatg tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat
     2161 ccaaacgact ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat
     2221 gatggtacga tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt
     2281 aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc
     2341 cacagtgtgg gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa
     2401 tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa
     2461 gcagtggaat tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc
     2521 catgattggg taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac
     2581 aaagaaacag gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta
     2641 caagatcctg atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg
     2701 ggctggccag atacggaaag cgaagattat aagcgatact tccctactag cactttagta
     2761 accggttatg acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt
     2821 actggcgaac gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg
     2881 cggaagatga gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat
     2941 ggagcagatg ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc
     3001 ttcagctatg aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca
     3061 cgtttcgtta tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa
     3121 aaatcagtgg ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact
     3181 gagctatttg atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg
     3241 gatgattttt gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct
     3301 gtttcaaaac aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta
     3361 ttgcatccaa tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga
     3421 aattctctag tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct
     3481 tctaaaggga tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa
     3541 gtgaatacac cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc
     3601 gatcaatttt tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg
     3661 acgatatcaa gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca
     3721 gaaatcttct tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa
     3781 aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa
     3841 cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat
     3901 tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa
     3961 gaatagaaaa agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat
     4021 cgttacagga aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata
     4081 aattttgtcg catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga
     4141 tcatgtatca agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct
     4201 ctttgtcata cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg
     4261 gaattgttga tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt
     4321 tcacgtcctg gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga
     4381 aaagtaaaaa cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg
     4441 agaagcctat tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa
     4501 acgttcaatg aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag
     4561 cttgtaaaaa aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt
     4621 attactgaat tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg
     4681 gacgtagcga ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta
     4741 ccaatgctga cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca
     4801 attgaagaaa tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca
     4861 ggaaatattg ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca
     4921 ccgctttatg tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg
     4981 ggagaagtat ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt
     5041 ttagggcaac atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt
     5101 caattggaaa agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga
     5161 tggccaggaa gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat
     5221 aatacgcatg caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa
     5281 atcaacattt tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg
     5341 cagcaagtac caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa
     5401 ttaagtgaag tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc
     5461 ggattagcag atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca
     5521 ctttatttca tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta
     5581 taatgcagtg atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga
     5641 agccacacaa gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa
     5701 attcttaggt gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt
     5761 tggcaaaaca agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt
     5821 ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga
     5881 acatcagatt cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt
     5941 gaagaagaac aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg
     6001 agcaaaacca gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa
     6061 agaaacattg attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat
     6121 cccggtagtc atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc
     6181 tggggttgtt tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt
     6241 tcgtatctta ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa
     6301 agaagtacct actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt
     6361 atcggatcag gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat
     6421 gtcgattgct ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac
     6481 tttgcatgag ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc
     6541 cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg
     6601 taccagctac gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca
     6661 tttggtttgt atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat
     6721 cggttcgctg aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta
     6781 ttgtgcagca aggatcgttt tagcgcataa tcatccttct ggtaatgtta ttccatcgca
     6841 gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt
     6901 actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat
     6961 ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta
     7021 attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt
     7081 tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta
     7141 aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta
     7201 ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca
     7261 gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct
     7321 taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga
     7381 gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtatt
//
LOCUS       DAXWLB010000135         7398 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXWLB010000135 DAXWLB010000000
VERSION     DAXWLB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.138"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            70..153
                     /locus_tag="ACGE4H_002222"
     CDS             70..153
                     /locus_tag="ACGE4H_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8365615.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4H_002223"
     CDS             278..1015
                     /gene="erm(B)"
                     /locus_tag="ACGE4H_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8365616.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1185..1445
                     /locus_tag="ACGE4H_002224"
                     /pseudo
     CDS             1185..1445
                     /locus_tag="ACGE4H_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1608..2273
                     /locus_tag="ACGE4H_002225"
                     /pseudo
     CDS             1608..2273
                     /locus_tag="ACGE4H_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2309..2554
                     /locus_tag="ACGE4H_002226"
     CDS             2309..2554
                     /locus_tag="ACGE4H_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8365617.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2657..3526
                     /locus_tag="ACGE4H_002227"
     CDS             2657..3526
                     /locus_tag="ACGE4H_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8365618.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3507..4241
                     /locus_tag="ACGE4H_002228"
     CDS             3507..4241
                     /locus_tag="ACGE4H_002228"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8365619.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4274..5182
                     /locus_tag="ACGE4H_002229"
     CDS             4274..5182
                     /locus_tag="ACGE4H_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8365620.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4H_002230"
     CDS             5179..5721
                     /gene="sat4"
                     /locus_tag="ACGE4H_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8365621.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5814..6608
                     /locus_tag="ACGE4H_002231"
                     /note="aph(3')-IIIa"
     CDS             5814..6608
                     /locus_tag="ACGE4H_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8365622.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6884..>7398
                     /locus_tag="ACGE4H_002232"
     CDS             6884..>7398
                     /locus_tag="ACGE4H_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8365623.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2592 a         1117 c         1554 g         2135 t
ORIGIN      
        1 tttgtgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
       61 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
      121 cagactaaaa acagtgatta cgcagataaa taaatacgtt agattaattc ctaccagtga
      181 ctaatcttat gactttttaa acagataact aaaattacaa acaaatcgtt taacttctgt
      241 atttatttac agatgtaata acttcaggag tgattacatg aacaaaaata taaaatattc
      301 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
      361 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
      421 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
      481 agaaaaatta aaactgaaca ttcgtgtcac tttaattcac caagatattc tacagtttca
      541 attccctaac aaacagaggt ataaaattgt tgggagtatt ccttaccatt taagcacaca
      601 aattattaaa aaagtggttt ttgaaagcca tgcgtctgac atctatctga ttgttgaaga
      661 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
      721 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
      781 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
      841 gctatatacg tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
      901 aaatcagttt catcaagcaa tgaaacacgc caaagtaaac aatttaagta ccgttactta
      961 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     1021 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gataaattat
     1081 tagatatact actgacagct tccaagaagc taaagaggtc cctagcgcct acggggaatt
     1141 tgtatcgata aggggtacaa attcccacta agcgctcggg accccttgta ggaaaatgtc
     1201 ctaagtgtgg caacaatatt gtattaaaaa aatcgtttta tggttgttca aattatcctg
     1261 aatgtaagtt tactttagct gaacatttta gaaagaaaaa actaaccaaa acgaatgtaa
     1321 aagaattact ggagggaaaa gaaaccctgg taaaaggaat caaaaacaaa gagaaaaagc
     1381 cctacaatgc cgttgtaaaa attggggaaa agggatatat tgattttatc tctttttcaa
     1441 aataaacatg gggctgaccc aaaagtagct cctatatgaa aaatagaggt ctttccattt
     1501 agatagtagt ggaaagacct ctatttatct tttgaaagtg aatacaaaaa taaagcacac
     1561 tctgctataa tataggtgat taatccattt gagagaggtg ctttatcatg tatcaaaatt
     1621 ataccataaa ccaactttgt ttgcctatag atcttgaaat taaattagaa gaaaatgatt
     1681 ttgctcatgc cattgtgcag tttgttgact ctattcccga tgaagtcttt cttccttatt
     1741 atcaatctat gggtagaccc caatatcatc ctcgtatgct actttccatt attctttgtg
     1801 cctatattca aggggtttat tcgggccgcc aaatacaaaa tatgctgata gatagtattc
     1861 ggatgcgcta cttatctcaa gagcaattcc ctaactttcg tactattaat cgttttcgcg
     1921 ttcatccgat tatgaacgat attctagatc attcatttgt tcaatttcgt gagctactgg
     1981 ttcaaagtgg cttaatcagt ggcagtgctc tatacattga tggtacaaaa attgaagcgg
     2041 atgcgaataa atactctttc gtttggaaaa aaagtatttt aaagtataag gatagccttg
     2101 atcaaaaagc ccttgaaaac taccaacaaa tggtggaaac aaaaatctta cctgagttaa
     2161 tagatgattt aaaggatgaa ctttctatag aagagattga aaggattcgt caatctttgg
     2221 aagaaaagga aaatcaatta attgaagcga ttgaccaaac cgaaacagta gaagggggct
     2281 gacccattat taataaggag tggacaacat gattgatgta aatgagctat tggaagaagc
     2341 tattagagaa actgaaaatc taaatgatgg cgaagttttt cttgttaaag atttattcaa
     2401 gggatatgtg gggaatagga tacccagaaa ggatcgactt ctgcttggaa cgttattttt
     2461 aaaccatgtt agcaaaatga atggtaattt aaaggctatt gaaaagactt catctaatca
     2521 gcagaggtat aaaaagacaa ttgataagtt ttaactatct tttgtgaaat taaaaaaatt
     2581 ataaatatgt tgagtagaag caaaagattt ggcttggaat ttttacagca gtgaatcgat
     2641 aaaaggagca tgcttgatgg tagataatat tattaaatca gtagcagaga aattatcctc
     2701 tctgtcttat atagaaggta ttgttttagg tggttcacgt gcaaggggca cccatacaga
     2761 ggattcggat atagatatcg gcatctatta caattcagaa tcatttgaca taaatactat
     2821 taatcaattc gctacaaagc tggatgatga gcatagaaat aaccttgttg tacctcccgg
     2881 agcatggggt gattggatta atggcggcgg atggttagtc ataaacgggt atcatgtgga
     2941 tttaatttta cgtgatatta aacgtgtgga acaaataatg aaagatacag agcacggaat
     3001 tgttactgcc aattatcaga ctgggcatcc ccatggttat attagtgcaa tgtatcgagg
     3061 agaattagcg attagcaaaa tactatatgc taagaatgaa agcttatgcg aattaaaaaa
     3121 acaggcagaa acttatccca atgctttgca gaaaagttta gttaactttt ttatgtttga
     3181 agcagggttc tctttaatgt ttgtaaaagc aaattcggga acagacgata aatattatat
     3241 tgcgggtcat gtttttcgta tagtttcatg tttaaatcaa gtgttatttg catgtaataa
     3301 tgcttattgt atcaacgaaa agaaagctat aaaactgctt gaaacttttg aacataaacc
     3361 tgaaaaatat accgagaagg taaatcatat ttttgaagta ctcggtatct cactttttga
     3421 atgctacgac atgaccgaga agctttataa tgaagtgaat gaaattgtat cggagataaa
     3481 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
     3541 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     3601 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     3661 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     3721 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     3781 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     3841 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     3901 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     3961 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     4021 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     4081 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     4141 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     4201 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     4261 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     4321 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     4381 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     4441 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     4501 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
     4561 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
     4621 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
     4681 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     4741 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     4801 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     4861 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     4921 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     4981 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     5041 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     5101 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     5161 agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat
     5221 aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat
     5281 tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag
     5341 gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga taagataata
     5401 tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac
     5461 cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc
     5521 agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg
     5581 cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa
     5641 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     5701 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     5761 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     5821 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     5881 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     5941 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca
     6001 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     6061 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     6121 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     6181 actccatcga catatcggat tgtccctata cgaatagctt agacagccga ttagccgaat
     6241 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     6301 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     6361 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     6421 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     6481 tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     6541 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     6601 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     6661 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     6721 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     6781 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     6841 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     6901 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     6961 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     7021 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     7081 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     7141 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     7201 atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt
     7261 gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac
     7321 tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc
     7381 ggaaagcaga atgataga
//
LOCUS       DAXWLB010000136         7217 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXWLB010000136 DAXWLB010000000
VERSION     DAXWLB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.139"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..518
                     /locus_tag="ACGE4H_002233"
     CDS             <1..518
                     /locus_tag="ACGE4H_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365624.1"
                     /translation="NGMPKEGEMFNLDKIITWRENWSKNILGELEVGRVYTNKEISEK
                     FKCSKQGGMRRSHQTNTLVLFSDQTGSNVYKDKWLNGILQYTGMGLKGDQVLDKNQNK
                     VLANSKSNFVKIHLFETFKPKEHTYLGEVYLAGQIYTVNEKDSSGNSRKVYKFPLALI
                     NQEQLIEDKDI"
     gene            499..945
                     /locus_tag="ACGE4H_002234"
     CDS             499..945
                     /locus_tag="ACGE4H_002234"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFF8365625.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1288..1611
                     /gene="relB"
                     /locus_tag="ACGE4H_002235"
     CDS             1288..1611
                     /gene="relB"
                     /locus_tag="ACGE4H_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFF8365626.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            1931..2158
                     /locus_tag="ACGE4H_002236"
     CDS             1931..2158
                     /locus_tag="ACGE4H_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFF8365627.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2174..3340
                     /gene="hemW"
                     /locus_tag="ACGE4H_002237"
     CDS             2174..3340
                     /gene="hemW"
                     /locus_tag="ACGE4H_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFF8365628.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3575..4618
                     /gene="hrcA"
                     /locus_tag="ACGE4H_002238"
     CDS             3575..4618
                     /gene="hrcA"
                     /locus_tag="ACGE4H_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFF8365629.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4712..5275
                     /gene="grpE"
                     /locus_tag="ACGE4H_002239"
     CDS             4712..5275
                     /gene="grpE"
                     /locus_tag="ACGE4H_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFF8365630.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5326..7155
                     /gene="dnaK"
                     /locus_tag="ACGE4H_002240"
     CDS             5326..7155
                     /gene="dnaK"
                     /locus_tag="ACGE4H_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFF8365631.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
BASE COUNT         2550 a         1169 c         1521 g         1977 t
ORIGIN      
        1 gaaatggcat gccaaaagag ggagaaatgt ttaatcttga taagattatt acatggcgag
       61 aaaattggtc taaaaacata ttaggtgagt tagaagttgg tagagtatat actaacaaag
      121 aaatttcaga gaagtttaaa tgttccaaac agggtggaat gagaagatct catcaaacta
      181 atactcttgt actgtttagt gatcaaacag gaagtaatgt ttataaagat aagtggttaa
      241 atggtatttt acaatatacc ggtatggggc taaaagggga tcaagtattg gataagaatc
      301 aaaataaagt tttagccaat tcaaaaagta attttgtaaa gattcattta tttgaaacat
      361 ttaaaccaaa agaacacaca tatctgggag aagtatattt agcaggacaa atatatactg
      421 ttaatgaaaa agatagttcg ggcaacagca gaaaagtata taaatttcca ttagctttaa
      481 taaaccaaga gcaattgatt gaagataaag atatataacc aggaagaaaa tcaaactaga
      541 catattcgta atttatctaa tgctaaatta gaggaagaag caagaaaagt tagtaattat
      601 aataaaaaaa tggctgcaaa atatgttaac gaagaggagt tttgcttctc aaaaacaaaa
      661 atatataaac gtaatccctt cgtatctgaa tatgttaaac gacttgcaaa aggtatttgt
      721 caactttgcc aagaaaaagg gccatttata aaagacggtg taccttattt acattgtcat
      781 catatagaat atttgtcgca aggtggaaaa gatgtgattg aaaactgtat cgcgttatgt
      841 cccaattgtc atgctagaat tcatgaactt gagttacctg acgataaaga aaaattgctg
      901 aagatcgttg aaaaaagaga aaatagcttt tctaaaatac cgtaatttat ttttatagga
      961 caaataagct aatgtgagct atgccttcta tataagacta agttctatta taagtaacga
     1021 agtaattctc gttccttcct gtagtaatat tttttaaata acctataggc ttcttcaaaa
     1081 gtacatcctc cagtaaattc taagtactgg aggattcttt ttctagtgtt gctttactca
     1141 tgaaaaagta gttggctact tcatcaaaat ttctgaactt ttttaatgta taataaagag
     1201 ctcgatcatt tactacaagg atgctagctc ctacattttc acagtttttt agcaagtaaa
     1261 tttttctaaa atcacatcag gaaatgcatg agattcttta agagaggcaa ttatagggct
     1321 atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc tttcttatat ggctgaattt
     1381 tatgggatta ctttatctga acttctaaaa aactattcga tggaacaact agaagatgaa
     1441 tacaatcgtc aaacggctga aattgcccat aaacattgga taagaagtgg gaaaacaaca
     1501 gtattcatgg aagaatcctt aatgaatttg gtgggctgga atgacctgcc gtttaaaatt
     1561 cacgcccgaa attcagaagc agctctgatt ggcaaatacg tgtattggta gagatagaag
     1621 ataatcaatt cgttgttatc gctattcaag tagggcaccg aagaaatgtc tatgattaag
     1681 agactgttta tttttacaaa aaaataataa ttggaatcta gttaaaactc ttgaagtgag
     1741 agatttgact agattttttt atgaataaaa taaggacaaa attgtttagg aagaatcatg
     1801 agtgtaaaaa aatcatccaa gaagtcgatt cttggatttt ttagtggatt tgttcgccaa
     1861 atacgtttag tatgcgtata tgagaggttg taagcaaagc gttgtggtct gagcacttaa
     1921 ggaggaatct gtgaaaatag cttttcatat ttttgcagat tttgacttaa tgccgaaggt
     1981 cagctttgcg attaccgttt atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa
     2041 taagcaagca tttttagagc taggtttcct tgattctaaa tatgcggctt atttcaatga
     2101 gttggtgctt gaacgcgatt ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg
     2161 agaaagaatt tcaatgatag aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat
     2221 tcatatccca ttttgcgagc atatctgcta ttactgtgac tttaataaag tttttttaga
     2281 agggcagcca gtagatgagt atgtggaaat gctgctgaat gaaatgagat taatgctgga
     2341 aagtcatcct acagcgaatt tggaaacact ttatgtagga ggcggaacac caacttcttt
     2401 atctgctaaa caacttgatc gactattatc aggtgctaga gagatcctgc cttttcaaaa
     2461 tggtaatgag tttactgtag aagcgaatcc tggtgatttg acccgtgaaa aacttcaagt
     2521 gatgaaaaac tatggcgtca atcgtttatc catgggagtg cagacatttg acaatcgctt
     2581 attgaaaaaa attggacgta aacatactgc agaagatgtg tatcaaacaa tgcaattttt
     2641 agaagcagaa aattttacca atgtaagcat cgatttgatt tatgcattac cgggacaaac
     2701 actagaagga tatcgggata ctttggaaaa agctttggct ttggatctgc ctcactattc
     2761 tttgtactca ttgatcctcg aaaacaaaac aatgttcatg aactgggtac gacaaggaag
     2821 actcgaactg cctgatcagg aaacggaaac cagaatgttt gaagagacga ttgaagcaat
     2881 ggaaaagcat ggacgtcacc aatatgaaat aagtaacttt gggctctccg gacatgaatc
     2941 tcaacataac ctcatgtact ggaacaatga ccactatttt gggtttggag ctggtgctag
     3001 cggttatctt ggagatattc gttaccgcaa caaaggacca atccaacact atttacgccc
     3061 attacgagca aatgaacgcc cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat
     3121 agaagaagaa atgttcttag gactaagaaa aaaaacaggg attactaaaa aacatttcta
     3181 tgatcgctat caaaaaacaa ttgaatcggt gtacggtgat gtgctaaggg aattagagca
     3241 agaagggtta cttgttaatg aagaagaccg tgtttatttg acaccaagag ggactttttt
     3301 ggggaacaat gtgtttgagc gctttttatt tgaaaaatag tttgaagaat atttgttttt
     3361 tatcgaaact tgaaaagaaa aagtttagaa agtttgacaa tgaggtattg aagtattgat
     3421 ataactggat ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa
     3481 tggagtcttt ctcttgacag gagaagggct tcttgctata tttataagtg aattagcact
     3541 tagaatatat gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat
     3601 tttacgtctg atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt
     3661 aatgtctgag ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga
     3721 agatgaagga ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg
     3781 ctatcgttat tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct
     3841 gctgattcgt cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc
     3901 tgctgagata ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa
     3961 agaccgccga ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat
     4021 cgtcgtaaca gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg
     4081 aagtcaggaa ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct
     4141 gatgacggtg taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca
     4201 gacgacagaa gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagaaaa
     4261 ggtttatgtc agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca
     4321 gttcaaatcg atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc
     4381 gatggatagc aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa
     4441 tatgagtatg attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt
     4501 gttagggcct actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag
     4561 agaactagca gctaaacttg ctgattacta ccgctcgctt gacttgtcaa actcgtaatg
     4621 tgaataacga agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca
     4681 gctgactgaa taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa
     4741 agaaatgaca gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg
     4801 tatttcagaa gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga
     4861 aatggaagat aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa
     4921 aaatgagcgt gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc
     4981 atcaattgac aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg
     5041 tttgaaaaaa ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg
     5101 aattgaaaaa atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca
     5161 aactgttcca gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg
     5221 ctacaaatta catgatcgcg tgttaagacc aactatggtc attgttgcac aataattgtg
     5281 cggatataaa acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc
     5341 ggtattgact taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa
     5401 atcatcgcta acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga
     5461 gaaatccaag taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca
     5521 tcaatcaaac gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat
     5581 acaccacaag aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat
     5641 ttaggtgaaa aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa
     5701 cgtcaagcaa caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat
     5761 gaaccaactg cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc
     5821 ttagtattcg accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt
     5881 gtctttgatg tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac
     5941 aaaatcatcg actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa
     6001 gacaaaatgg ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct
     6061 ggcgtatcaa gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg
     6121 catttggaaa tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa
     6181 cgtacaaaag taccagtacg tcaagcgtta aaagatgcag gtctgtcaca atcagaaatc
     6241 gacgaagtta ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt
     6301 aaagaaacag gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt
     6361 gctgctatcc aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt
     6421 acaccattgt cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc
     6481 aatacaacga ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca
     6541 gcagtagaca tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta
     6601 ggaagattcc aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta
     6661 acattcgata ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa
     6721 aaagaacaaa aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt
     6781 atggtgaaag atgcagaagc aaacgctgaa gcagataaag ctcgtaaaga ggaagttgac
     6841 ctacgtaatg atgtagacgc attgctattc tctgtggaca aaacattgaa agaattagaa
     6901 ggcaaagtgg acgaagaaga agtgaaaaaa gcagaaacag ctcgtgacga attaaaagcc
     6961 gctgttgaag caaacaacat cgaggaaatg aaaactaaac gcgatgcatt gaacgaaatc
     7021 gttcaaaact tgactgttaa actatacgaa caagctgcac aacaacaagc tcaagaaaat
     7081 ccagaagcag ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat
     7141 ggcgacgata aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc
     7201 tttttctttt gaaaaat
//
LOCUS       DAXWLB010000137         7158 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXWLB010000137 DAXWLB010000000
VERSION     DAXWLB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.140"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            18..899
                     /locus_tag="ACGE4H_002241"
     CDS             18..899
                     /locus_tag="ACGE4H_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8365632.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            912..2522
                     /locus_tag="ACGE4H_002242"
     CDS             912..2522
                     /locus_tag="ACGE4H_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365633.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2599..3258)
                     /locus_tag="ACGE4H_002243"
     CDS             complement(2599..3258)
                     /locus_tag="ACGE4H_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFF8365634.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3541..4206)
                     /locus_tag="ACGE4H_002244"
     CDS             complement(3541..4206)
                     /locus_tag="ACGE4H_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFF8365635.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACGE4H_002245"
     CDS             complement(4353..4958)
                     /gene="dhaL"
                     /locus_tag="ACGE4H_002245"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFF8365636.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACGE4H_002246"
     CDS             complement(4986..5996)
                     /gene="dhaK"
                     /locus_tag="ACGE4H_002246"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFF8365637.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACGE4H_002247"
     CDS             complement(5971..6366)
                     /gene="dhaM"
                     /locus_tag="ACGE4H_002247"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFF8365638.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6520..6840
                     /locus_tag="ACGE4H_002248"
     CDS             6520..6840
                     /locus_tag="ACGE4H_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFF8365639.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6952..>7158)
                     /locus_tag="ACGE4H_002249"
     CDS             complement(6952..>7158)
                     /locus_tag="ACGE4H_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8365640.1"
                     /translation="IKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTA
                     SVSYSLAGAGVLVAAAYGIIRKKK"
BASE COUNT         1880 a         1508 c         1390 g         2380 t
ORIGIN      
        1 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
       61 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
      121 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
      181 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
      241 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
      301 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
      361 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
      421 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
      481 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
      541 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
      601 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
      661 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
      721 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
      781 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
      841 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
      901 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
      961 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
     1021 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
     1081 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
     1141 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
     1201 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
     1261 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
     1321 gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt tttttccaag
     1381 cggattcgat cactgcaaaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
     1441 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
     1501 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
     1561 ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta
     1621 caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
     1681 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
     1741 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
     1801 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
     1861 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
     1921 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtagtc ttgatttgtc
     1981 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
     2041 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
     2101 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
     2161 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
     2221 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
     2281 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
     2341 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
     2401 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
     2461 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
     2521 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
     2581 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
     2641 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
     2701 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
     2761 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
     2821 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
     2881 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
     2941 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
     3001 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
     3061 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
     3121 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
     3181 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
     3241 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
     3301 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
     3361 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
     3421 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
     3481 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
     3541 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
     3601 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
     3661 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
     3721 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
     3781 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
     3841 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
     3901 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
     3961 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
     4021 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
     4081 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
     4141 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
     4201 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
     4261 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
     4321 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
     4381 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
     4441 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
     4501 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
     4561 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
     4621 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
     4681 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
     4741 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
     4801 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
     4861 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
     4921 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
     4981 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
     5041 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
     5101 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
     5161 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
     5221 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
     5281 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
     5341 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
     5401 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
     5461 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
     5521 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
     5581 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
     5641 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
     5701 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
     5761 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
     5821 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
     5881 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
     5941 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
     6001 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
     6061 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
     6121 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
     6181 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
     6241 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
     6301 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
     6361 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
     6421 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
     6481 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
     6541 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
     6601 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
     6661 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
     6721 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
     6781 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
     6841 gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat
     6901 tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt
     6961 tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact
     7021 gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc
     7081 tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga
     7141 atgcaaaata tcttttat
//
LOCUS       DAXWLB010000138         7056 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXWLB010000138 DAXWLB010000000
VERSION     DAXWLB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.141"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(4..534)
                     /locus_tag="ACGE4H_002250"
     CDS             complement(4..534)
                     /locus_tag="ACGE4H_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFF8365641.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(633..983)
                     /locus_tag="ACGE4H_002251"
     CDS             complement(633..983)
                     /locus_tag="ACGE4H_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFF8365642.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="ACGE4H_002252"
     CDS             complement(1140..2669)
                     /gene="ahpF"
                     /locus_tag="ACGE4H_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFF8365643.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="ACGE4H_002253"
     CDS             complement(2679..3242)
                     /gene="ahpC"
                     /locus_tag="ACGE4H_002253"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFF8365644.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3386..4048)
                     /locus_tag="ACGE4H_002254"
     CDS             complement(3386..4048)
                     /locus_tag="ACGE4H_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365645.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4232..5878
                     /locus_tag="ACGE4H_002255"
     CDS             4232..5878
                     /locus_tag="ACGE4H_002255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFF8365646.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5869..6951
                     /gene="uxuA"
                     /locus_tag="ACGE4H_002256"
     CDS             5869..6951
                     /gene="uxuA"
                     /locus_tag="ACGE4H_002256"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFF8365647.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         2265 a         1331 c         1309 g         2151 t
ORIGIN      
        1 tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat
       61 gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa
      121 caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc
      181 ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag
      241 aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat
      301 caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg
      361 gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc
      421 gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac
      481 agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga
      541 aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa
      601 atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt
      661 ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta
      721 tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga
      781 ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat
      841 tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt
      901 atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc
      961 gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac
     1021 ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt
     1081 gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct
     1141 caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa
     1201 acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata
     1261 ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca
     1321 tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt
     1381 gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg
     1441 ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta
     1501 tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt
     1561 gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca
     1621 atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt
     1681 ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg
     1741 gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct
     1801 ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga
     1861 ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca
     1921 atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt
     1981 cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat
     2041 acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca
     2101 acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt
     2161 ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc
     2221 gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg
     2281 caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg
     2341 attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc
     2401 agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct
     2461 ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag
     2521 attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca
     2581 ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg
     2641 agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca
     2701 aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg
     2761 tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg
     2821 attccatctg cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt
     2881 cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga
     2941 gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat
     3001 gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc
     3061 aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca
     3121 gctggataga agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa
     3181 aattctcctt gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac
     3241 atagtaaaac ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc
     3301 aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa
     3361 aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa
     3421 cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt
     3481 ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc
     3541 atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt
     3601 catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata
     3661 aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt
     3721 ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca
     3781 tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa
     3841 tttttgaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt
     3901 gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt
     3961 cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat
     4021 aagcttgaga ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt
     4081 agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta
     4141 taaatagtaa aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca
     4201 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
     4261 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
     4321 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
     4381 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
     4441 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
     4501 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
     4561 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
     4621 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
     4681 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
     4741 gaagccatca catacaatga gtattcttgc tgcgctactt tataaacgtt ttttatctgg
     4801 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
     4861 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
     4921 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
     4981 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
     5041 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
     5101 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     5161 agtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     5221 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     5281 agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     5341 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     5401 aattactgtg cgcttttcta atccaaatat tcctgatatg cctcagcgaa tcgccacgga
     5461 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     5521 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     5581 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     5641 agactttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     5701 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     5761 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac cgaatgccgt
     5821 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     5881 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     5941 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg
     6001 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     6061 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     6121 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     6181 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     6241 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     6301 cgcatcgaac attccagcaa tgaatttgaa ttgccgggat gggaacctga gcgcttagct
     6361 catatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     6421 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     6481 gtacatccag atgatccgcc atactcgatt tttggattac ctcgaatcgt aaagaatcga
     6541 gaagatttag attggttgtg ccgcatagta gacagccctt ataacggaat cactctttgt
     6601 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgt
     6661 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     6721 ttctatgagg caccgcataa atccgaatac ggttcattag acatgtaccg catactaaaa
     6781 acaatgtatg acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg
     6841 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     6901 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     6961 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttggagagta taaaatattt
     7021 tgtgtaaatg aaaaaatcca tacaaaaaag gaagtc
//
LOCUS       DAXWLB010000139         7019 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXWLB010000139 DAXWLB010000000
VERSION     DAXWLB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.142"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            169..1026
                     /locus_tag="ACGE4H_002257"
     CDS             169..1026
                     /locus_tag="ACGE4H_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8365648.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1019..1921
                     /locus_tag="ACGE4H_002258"
     CDS             1019..1921
                     /locus_tag="ACGE4H_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFF8365649.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1954..2304
                     /locus_tag="ACGE4H_002259"
     CDS             1954..2304
                     /locus_tag="ACGE4H_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8365650.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2353..3276
                     /locus_tag="ACGE4H_002260"
     CDS             2353..3276
                     /locus_tag="ACGE4H_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFF8365651.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3354..4247
                     /locus_tag="ACGE4H_002261"
     CDS             3354..4247
                     /locus_tag="ACGE4H_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8365652.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4285..4848
                     /gene="amaP"
                     /locus_tag="ACGE4H_002262"
     CDS             4285..4848
                     /gene="amaP"
                     /locus_tag="ACGE4H_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8365653.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4862..5050
                     /locus_tag="ACGE4H_002263"
     CDS             4862..5050
                     /locus_tag="ACGE4H_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8365654.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5063..5965
                     /locus_tag="ACGE4H_002264"
     CDS             5063..5965
                     /locus_tag="ACGE4H_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8365655.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5997..6239
                     /locus_tag="ACGE4H_002265"
     CDS             5997..6239
                     /locus_tag="ACGE4H_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8365656.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6495..6866)
                     /locus_tag="ACGE4H_002266"
     CDS             complement(6495..6866)
                     /locus_tag="ACGE4H_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365657.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2565 a         1189 c         1393 g         1872 t
ORIGIN      
        1 ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg ataaaaaaat
       61 tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat gaggaatatt
      121 ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat ggagaagaga
      181 tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta cttgagcgtg
      241 atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt ttatgtgatt
      301 gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa taaatattca
      361 agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga cttcctagtc
      421 agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt attagctaaa
      481 gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga cgatatcgta
      541 aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga tccaggccaa
      601 gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa ttcaggagtg
      661 atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac tatccattac
      721 ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa tgctgtactt
      781 tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct gatttttgaa
      841 cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct
      901 tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga tcatccgtat
      961 accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg cgagatcaat
     1021 gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac cacatttagc
     1081 tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc ggttctatgt
     1141 gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc
     1201 acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta caaatgtggt
     1261 aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg aattgttccg
     1321 cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt tacaagatat
     1381 ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg aagacgctgg
     1441 gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc
     1501 aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt
     1561 agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg cattaaatgc
     1621 tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag gctatatcat
     1681 ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag aaactcgcaa
     1741 caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg attttgaagg
     1801 gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt gtgtagctaa
     1861 attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata
     1921 atagaagacc acaactgaag agggggagta ggaatggaaa ttacaatgtc taaaaatgca
     1981 gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat
     2041 gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc
     2101 attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc attattggac
     2161 atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat ggacttcaac
     2221 aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta taatatcaaa
     2281 ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag gtatacattg
     2341 agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa aaaatataat
     2401 tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga agaattcgag
     2461 cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg gatggagcgc
     2521 ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct agatcagtca
     2581 gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga taatctcttg
     2641 attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa gcatggaaat
     2701 catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta cacccattac
     2761 gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc agctcgtaaa
     2821 acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat cgtatatatc
     2881 gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc tgaaactttc
     2941 atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca gcgacaaaca
     3001 gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct attcaacggg
     3061 atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc attaatcatt
     3121 tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt accgggtaaa
     3181 agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc agtagtttta
     3241 ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa acaggtatca
     3301 aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca gcaatgacag
     3361 aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct aaacaagatc
     3421 aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga gaagaatcct
     3481 atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga gattcaggga
     3541 tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct cttcaattct
     3601 tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagat gcaggacgta
     3661 agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa atcatagata
     3721 aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga cagcaaattt
     3781 cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt atgataaaca
     3841 tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca ggaggagcaa
     3901 tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta gattacgcag
     3961 caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt gcacctaaag
     4021 gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa ctagacgaag
     4081 gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa cgtgcaggtc
     4141 agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca agttatgtta
     4201 cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa gttgatcgtt
     4261 tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg agtgatcgga
     4321 acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac ctatccattg
     4381 ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt gcaacaatat
     4441 gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact tgttttcctg
     4501 ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga gttgacgttg
     4561 gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga attcatccat
     4621 tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt aaaaggggag
     4681 ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga aatcgaaaat
     4741 gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa atacacaggc
     4801 tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg ggggaccaaa
     4861 aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac ttggattgat
     4921 attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct tgatcatggc
     4981 agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag atcactattt
     5041 taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca ataaaatgaa
     5101 caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat caaatggtgt
     5161 gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag tagtccctaa
     5221 cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac ctcatcatcc
     5281 aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt ctaaacctgt
     5341 ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta gttctgacca
     5401 cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa agcatgattc
     5461 tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg tgcaaaaaat
     5521 catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg gtttcttctc
     5581 taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg aaacagaagt
     5641 cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca aagatatcga
     5701 aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata tgacacattt
     5761 agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag aatttgaaaa
     5821 agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg gtgaatttgc
     5881 ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa aagtaaaaga
     5941 aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa cgaaacatgg
     6001 gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga gcaatcacga
     6061 ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt ggatcagcac
     6121 ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca atcgtgccat
     6181 cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga aaactgtaaa
     6241 aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa gaacagccaa
     6301 acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga tgcaagcttt
     6361 tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag cctcttattt
     6421 atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt ctttattgat
     6481 acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat aggaaaggtg
     6541 aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg gatcgtatga
     6601 tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa gacaaggaag
     6661 aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt aaagtaatct
     6721 agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc atgttgctct
     6781 gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac gagtaatgaa
     6841 agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt tcgccctcat
     6901 tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg ttatctaatg
     6961 tatttttaaa taaataatca ttattatcaa aataatgtta aaaataaaaa agaattgtc
//
LOCUS       DAXWLB010000140         6897 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXWLB010000140 DAXWLB010000000
VERSION     DAXWLB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.143"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(735..1076)
                     /locus_tag="ACGE4H_002267"
     CDS             complement(735..1076)
                     /locus_tag="ACGE4H_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8365658.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1076..1306)
                     /locus_tag="ACGE4H_002268"
     CDS             complement(1076..1306)
                     /locus_tag="ACGE4H_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8365659.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1593..1931
                     /locus_tag="ACGE4H_002269"
     CDS             1593..1931
                     /locus_tag="ACGE4H_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365660.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2136..2698)
                     /locus_tag="ACGE4H_002270"
                     /pseudo
     CDS             complement(2136..2698)
                     /locus_tag="ACGE4H_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3430..4011)
                     /locus_tag="ACGE4H_002271"
     CDS             complement(3430..4011)
                     /locus_tag="ACGE4H_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8365661.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4194..4820)
                     /locus_tag="ACGE4H_002272"
     CDS             complement(4194..4820)
                     /locus_tag="ACGE4H_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFF8365662.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4842..6173)
                     /locus_tag="ACGE4H_002273"
     CDS             complement(4842..6173)
                     /locus_tag="ACGE4H_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFF8365663.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6328..6729)
                     /locus_tag="ACGE4H_002274"
     CDS             complement(6328..6729)
                     /locus_tag="ACGE4H_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8365664.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         1992 a         1415 c         1106 g         2384 t
ORIGIN      
        1 atagtgtacc taaaagctct actgacataa gatcgtgtaa tcggtaaagt aattttatta
       61 attatacggg atgtaatcaa ttagagaata aactaatcat aaaaggtact tttacattca
      121 taattgtcgc tttgagattc tactttggtt cactatctga cccaattgca cattttcgat
      181 cattacaagt attggtgtcg ttagaacaac taaataaaaa ctagcggtag atttttcaga
      241 atctatgcta atttctgtat ggatgttcca gtgatggcaa atttctcttt tcattgtttt
      301 tcttgtcgca tgatatgcct ttttgtgtta ttcagttaaa ctatagaaat tctttcctag
      361 acagatcttg atcccaaatt ttacgttttt gagcatcgat atagtttaaa atgacattac
      421 tcatatctaa agagtttaga ttttttagga ctaaaactgt cagttgttct ttttcttctt
      481 tctgttaaag tattgtcgcg acttttcgta tacgttaaga tatgtcgtga aaaaacaagt
      541 tttgtgacac aaaaaaacga atgacttgag gaaatcacct caatcattcg tttaaaatgc
      601 tatatattct ttagtttaag atacggaaat taatgccatc gaccacacta gtagaatgaa
      661 cgacatttac ttggcctttt tttaatttcg ttatgtttag catataaaaa tattatagag
      721 tttacttaca atgcttaaaa catatactgt ataatctgat caattttagc catgtcttgc
      781 aatggtaagt tttctatttt tttgagtttt cgttccttaa aatcaagaga ttttaattgt
      841 gtaataatta cttgtccttt tatatctagc tcttcaggta atgagtaacg tgtcggtaaa
      901 ttctttatag tggaagtaat tggacatatg accgaaaaca ttgttttacg gttaaattca
      961 taacgagaga cgactagtcc tggtcgtctt ttttgaattt cttttcctac ggagggatca
     1021 aaatcaatcc atacaatatc acctttttta ggaatataaa ttctttcttc tgacactata
     1081 aaatttctct cccttcagga gttaaatctt cccattcgtc tttctcataa tactcggcct
     1141 cttccccacc actaaatgga tcttcaattt ttggaactaa cgtaattgtt ccatcatcag
     1201 aatacacaac gatatattct tggttttctg aaggtttctt tccgttatca gaaggtaaag
     1261 ttaccactac agaacttcct tgtaatctag attttgtaac aagcattatc atcactccct
     1321 taataaaagt atactatata acactaagta acgcaaaatt cccctttgta aactatctag
     1381 aaaattgttt tgtttaaagt atatcttatt ggatataaat agaaataaat tgtagccagt
     1441 tcattttttc tttaactaaa aaaataatcc aagaaacatt gaacctatgg atccaaaagc
     1501 tacccaaata ctatagctaa ccccccgcta ccttcaattt tgcagataaa acgctttggg
     1561 agaaaatttc aaagacaaca ggccatccta tcatgcccga ttataaacaa aaagctggct
     1621 attccttttg ctctgacaaa tttcaattgg gaaagttcac gaaactagca ggtgaagaag
     1681 tttcaactgt tcggatcaaa gaatgcccgc ttcaaataga aacattggtt caacgaattt
     1741 caactagaca agactttgct attgtagaac gtgagatcgt agctattttt gtagaagaag
     1801 ggattttaca tgatccaact catatcaatg tggataaatg gcaacctctt atctataaat
     1861 tcagagaata tacaactgcc aaccagtcgt taactttgaa ttttagattc caagaattca
     1921 aagagtcata atgataaaca tcgttaggta taaataaaaa agactcgcag cacgagtaaa
     1981 aaacaaatag aagctataca ttagaaaggt cgactgtgac cctatagatg cagtatatgc
     2041 agcttatgag tatttcttct gttctctttg tttttgggat ctgtgctgaa gtctttttcg
     2101 ttttttgccg ctctttcttt gaaaaatcga ttatgttata ccttctccat tactaattca
     2161 ggcatctgct gagtaatgat ttttcgcgca aaagaaatat ctgttaccca aggttcgatt
     2221 tgttcttcag tcaagacaag cggcattcgg tcatggattg gtgcgactgt gcggttaggt
     2281 tcagtagtca ttatgattga ttctgccatt ttttcatcct gatggctacg ataaaaaccg
     2341 ccaatataca ctattttttc cttatttgaa aacataaatt tgtctttttc ttcattccac
     2401 tcattgggaa aacacaacga ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta
     2461 ctgtctctgc acgggcatta atcatcaatt gtcctttttt aaatccggta aatccccact
     2521 tcatgatttc cggagtgagg atgttttctt cctcttttct taacgtcaca acaagatttg
     2581 acggaaaaac ttctccagct gctaattctg ctgcctgctt cggttgtttc gtctgggctt
     2641 ctcgataata attctgaagg agctctgact ctagatcaaa aaaataacga ccacacatcc
     2701 taatccctcc caatcttttt ttctatcata cactgcaaca agaagttcgc accaacaaaa
     2761 cgctttcaat ttttgtagaa ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg
     2821 gtattctcct gccaactcct cggtcacaag tcacaatatt caacaaacat gagaagctgc
     2881 tttttgaaat tgttccttgt gactcggagt gaaacggcag gcttacgacc tctgggtaaa
     2941 cggtgttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagct
     3001 attttcacaa aatttttctt aatactcgaa actaagcgac tttttcacaa cctcttcctt
     3061 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagc
     3121 agctattttt atttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc
     3181 cactaaacga tgttcaatca gctaaccttc ggtattagct caaaaaacgg caaaatatga
     3241 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa agcgctgatt tcacaacctc
     3301 taatacacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt
     3361 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
     3421 ctctaattat taaagaacct tttgcgaagt taacaatctt tctttgatca attgaaaatt
     3481 ctgcctaagt agtgatagat cctgtgtgac tttcaacaat aagatacttc cctggatttc
     3541 agcaatcaac gctttggctt gacggtctgc ttcttcatcg gacagttttt cttcagaaag
     3601 tgccaggata ttttgtttcg tatacttgac ccattgattg actaattcta gtagcaacag
     3661 acggtattct tcatccactg tactcaactc aataatcaaa ttacctacag gacagccatg
     3721 ataagtgatc tgtgtctcat gatcagcgaa aatccagtcc aacatttctt ctaatgcttc
     3781 ttctggctgt tttccagcat ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat
     3841 atgcgcatgg atcaccgcta gacaaacttc tttttttgaa gaaaagtaat aataaaattg
     3901 accttttcct gtatcagaag ctgctagaat atctcggatt gacgtttcat gatagccttt
     3961 tttataaata agatctttgg tcgcttccaa gatcttttcc tttgttttca atgatattct
     4021 cctttttcat ttctcatagt cctatattat acagtaaatt catcattttt tcttcttaat
     4081 cgtttaaaaa agactgtgcc aaattatctg acacagccct cactataaaa ctgttctcat
     4141 atagctagat gttcattgat tcaagaactg cgaggacaaa agcaattcct atcttatgat
     4201 agttgctcta atacttcctc tgttgtcatc acttgatctg caataaagcc aaagttcgtc
     4261 aacgctgcat ccattgcttc ttggccagga gcaccaattg catctgtcgc taccacaaca
     4321 ttgaatccac tctctaccaa ttcacgcata tgggaatcgc tacacaagtt tccgttcatt
     4381 ccaccaagaa ttactgtgtc gattccattt tttctcaatt ggagcgccaa atcattgctt
     4441 tctggtccaa agattttatg aggagagctg atgatcgtat tttcatccaa ataaggtttc
     4501 aattcttcga taaaatcggc tcctgaacct tcttccactg attcatattg actttttcta
     4561 gcaaacatct gctgctcaag catcatatgt tctcctgcac cgccaaattg ccatttatgg
     4621 tcatgtgggt aaaaataatg aggagaaata aagagtttgt atcctttttc ttttgcttgt
     4681 tttaccattt caatcatatt ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa
     4741 ccaaagccac cgcctgctgg tttcaagaac tcgacttgcg ggtcagtgag aactactgct
     4801 gtatttttat tattgaacat ggtggattct ccattcgtta tttattttaa tacatcattt
     4861 agagcttcac tcattgtcgg atgagaatag atttgatctc tcaataacgt ataaggaagt
     4921 tttccattca tagctagcga aatcaagttg atgacttcat gagaatcttc cgcatagata
     4981 cttgccccta gaatcaagtc agtttcaggg tcgactaaaa ctttgaatac acctttagga
     5041 tcttctaata cttgtgcttt agggacaccg cttgtcatat acttgaataa tttgtaattg
     5101 acgccttgtg ctttggcttg tttttctgtt aaacctacat tggataaagt cggagtgata
     5161 aatacactgt aaggcacagc tttccggtta ttcgttgttc gtttattctc gcccttcaac
     5221 tgatccataa tgattcgata atcatctaga gagatataag tgaactgcaa tccgcctttg
     5281 acgtctccaa tcgcccaaat gttgtctgca tctgtacgaa ggtattcatc cacagcgatt
     5341 gctccgcgat ctgttagttt aacatctgta ttttctaatc caagtgatgc tgtacttggt
     5401 tttcttccag ttgcagccaa aacttttgac gcttggattg ttacttcttg gccatcttct
     5461 gtaaaggtga cggcagctgt tgattcttgg tctgctactt gatctacaga tacacctaga
     5521 tggaattcga ttcccgcatt tgtcatgtct tctaaaatca tttgcgcgat atcttcatct
     5581 tcacgtggaa gaaattcaga atgggcatca agaacgacaa ctttagatcc gtattcattg
     5641 aacattgatg caaattccaa gccaatatat ccagccccga tgatcactaa tttttccggt
     5701 aattgttcct gatccatcgc ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct
     5761 ttgattggta aaatgattgg ttcagaacca gtattgatga aaatccgttc gccttcaaca
     5821 atcacttttt caccattatt cgtcacttcg atttgatggt tagaaagaaa acgtgctgta
     5881 ccgtccatca ctgtcccagt tgcctcatcg ctgatcatat gataattttt ggcgcgaagc
     5941 atgcccgtca atttttcttt ccgagcaaca gcctctgtaa agtcgattcc tctttctcca
     6001 ttgaaaatca atgatttaga aggaatacag ccgatgttga tacatgttcc gccatacata
     6061 cgtgttgact tttcaacgac taaaaccgat tcaccttttg aagctaacgc cttcgctaaa
     6121 gtcttaccgc cctttccaaa accgatcaca atattttgat atttttccat catttttccc
     6181 tcgcttcttt tttacctacc gattggtaca aaaatatgat aaccgataat tgtctattct
     6241 ccaaatcatc tgctttattt gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt
     6301 tagtctggtt cgatttcctt tattcttcta aacaatcgtt acttctacat cgatatttcc
     6361 tctagttgct ttggagtaag ggcagaattc atgcgcatct tctgcaattt tttgggcttc
     6421 ttcttcgctg atgccatcga tatgcacttc taatactaca ccgatctgat agccgccatc
     6481 atctaaatta taaagagaga cacgtgcttt tactgtggac tctgctgtca ttcctgcttc
     6541 tttgatcata tgttgtaccg ccccattgaa acaagaagca taacctgctg caaataattg
     6601 ttctggattt gttccttctg catggattcc tggaggagtg attttcatat gcatcgagcc
     6661 atttggtgcc tcgacttctc cattgcgccc gccatggttg atcattgctg tttcgtatgc
     6721 ttttttcatt gtactgctcc tttcgattga ccatttgcta cttggctata attatagtgt
     6781 atgacaagta aggcgctttc tacaaaaatt ctgcttattc gaataccaga atgccgtctt
     6841 ctactatttt tccaaaaaac aaaaaaatac tactcgtgaa caacagcgtc atatttg
//
LOCUS       DAXWLB010000141         6870 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXWLB010000141 DAXWLB010000000
VERSION     DAXWLB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.144"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            33..656
                     /locus_tag="ACGE4H_002275"
     CDS             33..656
                     /locus_tag="ACGE4H_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365665.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACGE4H_002276"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACGE4H_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFF8365666.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACGE4H_002277"
     CDS             2152..3033
                     /locus_tag="ACGE4H_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365667.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACGE4H_002278"
     CDS             3036..3806
                     /locus_tag="ACGE4H_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365668.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACGE4H_002279"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACGE4H_002279"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFF8365669.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACGE4H_002280"
     CDS             5117..6280
                     /locus_tag="ACGE4H_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFF8365670.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACGE4H_002281"
     CDS             6397..6807
                     /locus_tag="ACGE4H_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8365671.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
BASE COUNT         2308 a         1173 c         1502 g         1887 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg
//
LOCUS       DAXWLB010000142         6615 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXWLB010000142 DAXWLB010000000
VERSION     DAXWLB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.145"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            715..2592
                     /gene="ltrA"
                     /locus_tag="ACGE4H_002282"
     CDS             715..2592
                     /gene="ltrA"
                     /locus_tag="ACGE4H_002282"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8365672.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            2723..3100
                     /locus_tag="ACGE4H_002283"
                     /pseudo
     CDS             2723..3100
                     /locus_tag="ACGE4H_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            3100..4305
                     /locus_tag="ACGE4H_002284"
     CDS             3100..4305
                     /locus_tag="ACGE4H_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFF8365673.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            4321..4965
                     /locus_tag="ACGE4H_002285"
     CDS             4321..4965
                     /locus_tag="ACGE4H_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365674.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            4976..5782
                     /locus_tag="ACGE4H_002286"
     CDS             4976..5782
                     /locus_tag="ACGE4H_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365675.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            5804..6034
                     /locus_tag="ACGE4H_002287"
     CDS             5804..6034
                     /locus_tag="ACGE4H_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365676.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
BASE COUNT         2468 a         1030 c         1414 g         1703 t
ORIGIN      
        1 aaaagctgtg ggttgcaagt agtctaaaaa ctattgtaat aactcttata acgagaagtc
       61 aaatgagtgg gtaacgccac aaagggcttc cactgatact ccgacatgca acaagtagtt
      121 tatgcagacc aacgttgtgt gaagctcggt gaagtcggct gaaggatacc gtaaggtaaa
      181 agaaatagtg aataccacga aagttctcta gaggtaaagg gtgtatccga taagtcgggt
      241 gtccaaaaaa acttaatatg gtgagtatgt acaaacgtac tgacgaacta gcgaatgtac
      301 tgtccgaaag gcatagctcc tagaaatggg agagacagtg agcaaaaaat cactcattgt
      361 gctaacttgt ggttgagtaa aagtcgttgt tatgaaagac catgctgcgt tacaggcgta
      421 ggagaagtta gggggcaaaa gctagcatct aagttaaggc atgtggatag atataaggga
      481 acaggggaag ccatcaacat ggagagtgca tactcgatga agtggtagta agaaatatgt
      541 gaatgacaaa taacttactt tagtgatggt gaaagtagag tcacgactga tgaaggttct
      601 gtaatggaac tggaggaaca ggctcgattc gtctgtaaaa gtcatgaaac ccgttcaacc
      661 atgggattct cgtatgacta aaaaggtcac gagccaattg gagcgtgata aaccatgaca
      721 aaagaaacaa gtccaaaaga taggaagttg cggcatgcag aatattatgg catgacggaa
      781 atcttcgata agatatactc caaaagtttg aagggagata atttcaagaa tctaatgcag
      841 attatcattt ctgataacaa cattgtccta gcatatcgta atatcaagag aaatcacggt
      901 agtgtgacaa gaggagaaga tggagtaacg attaaagata ttgaaaatct caaccgtaat
      961 gaattcgtag aaattgttag gaagcgattc gcacattata accctcggaa agtaaggcgt
     1021 gtagaaattc ctaaatataa tggtaaatta agaccgttgg gtattccatc aatatgggat
     1081 agaatcgcgc agcaatgcat cttacaagta ttagaaccaa tatgtgaagc aaaattcaat
     1141 aatcacagct atggtttccg cccaaataga tcagcagaac acgcaatagc agattgtatg
     1201 gcccgaatga atcaaagtca catggaatat gtggtagatg tagatatcaa aggtttcttc
     1261 gacgaagtca atcatactaa actcatgaga caaatctggt ctatgggtat acgagacaaa
     1321 cagcttctct taatcattag gaaaatgtta agagcacccg tagtacttcc aaatgggaag
     1381 ttgatatacc caacaaaagg aacaccacaa ggcggaatac ttagcccatt acttgcgaac
     1441 atcaatctca acgaatttga ctggtggata gcaaatcagt gggaggaaaa agatgccaaa
     1501 gagctaaaac atcattttag gaaaaatgga gcttgggata aaggtaatat ctatcacaag
     1561 ctcagaaaga gtacaactct aaaagaaata tacctagtga gatacgcaga tgattttaaa
     1621 attttctgta gaaattataa tgaagcgaaa aaagttttct atgcaactaa aatgtggtta
     1681 caggagcgtc taaagttacc aatatctgaa gaaaagtcaa aaatcacaaa tctttcaaaa
     1741 gaagaaagtg aatttttagg ctttacgatg aaattagtga ccaaaggtaa caaaaaggtt
     1801 gttaatacgc atataggtac aaaagcacaa agcatgatta aagtgaaact aaaaaaacaa
     1861 atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct ttatctatat tggagtctat
     1921 aacagtatgg ttattgggat tcataattac ttcaaaattg cgactcattg tagtgtagca
     1981 atgcgaaaaa ttgcaagaga actcagacca agttttgaaa tacgactacg agaacaagga
     2041 atctcaaaag aaggacaata cggaaagtac gaaggataca agccttacct acggtcagga
     2101 caaatacgat atataagagg gtttccaata ttaccagtag ggtatattca acacagacat
     2161 ccgatgtgta aaaaaagaaa tattaataaa tacaccgaag aaggtaggga agccattcat
     2221 aagaagcaac aaaccgtaga agaatggaag atatcgtggt tgtgtagcaa tccagtgctt
     2281 agtaagagag caacgattga atttgcggac aatcgaattt caaaattcat cgctcaaaag
     2341 ggaagatgtg cagtaacagg tgaggagctt atcttatctg aaatgcattg tcatcatatt
     2401 atcccttatc atgaaagcaa aagtgatagc tatgaaaacc tagtgattgt tactgaggag
     2461 gttcatcggg taatacacgc tacacaaagt gaaacgattg aggaacttct aaaatatcta
     2521 aaattaaatc caaaacaaaa agaaaaactc aatgagttga gattaaaagt tggtaacgaa
     2581 gaaatatctt aataaatatt tgtaggacgg gaaaacagaa tgtcttttat agacgaggga
     2641 acgccgtatg cggtgaaagt cgcacgtacg gtgtggactg ggggaaaaga cggagataat
     2701 atcaaactct tacctatcag tatcgattaa tccagatttt attgaaacgg taaattatgt
     2761 cacaaacttt caacgagaaa tgcgtaaagt agaagcaatg actgttttag ctacacaatc
     2821 cccacaagaa atggtgccag aaaatatgtc ttctgataac ttctcgaaac tgaagaaagt
     2881 ctttgaattt tcttctacga agatctttat gcgtatggat gaatctattt tacgtcatat
     2941 agaaggatta gtgggtaaat caatgacgaa ctcagaatta gaatccattc ctcaacaaaa
     3001 tcaaggagac gctgtgatca attttggctc aaaagaaacc attcgtgttc attttagtcc
     3061 gaacaaacgt caattaaaat tgtttgatgg aggaaaataa tggaaaacca aaatgaaagt
     3121 ttaatcaagc agtatgttaa gcgtcgtgca aagcggcgct tatttttgtg gttgtttggt
     3181 acttctgcag gtttgattac tattttgatc actgtgttcg tgaccctatt tctaatactt
     3241 gcggcgggct cgattgataa ttcggattca gattcaagca gtggtggcga agcttttaca
     3301 ggagaatatt cggaaggctt accgatttat aaagaaatca aaggaagagg ccctttttct
     3361 gatgagattg cgcaatatgc cgtaggtgca gcagtgaaat ataaattatt accaagtgtg
     3421 atcctttcgc aatacggtta cgaatcagcg tttggtacat cggcgtccgc aagaaatgat
     3481 ctcaactatt ttgggattac ttggtttgat ggttgtctgt ttcctaaagg aactgcacga
     3541 ggaattggtg gcattgaagg tggctggtac atgaagtttc ccaactcaaa agctgcattt
     3601 tcctattacg gctttatggt agccacacaa tccaatttta atgcttgtgt aggaaataaa
     3661 agccctggcg caagcttatt gatcttagga cgaggcggtt atgccgctgc aggaattacg
     3721 gaagatagtc cgtattatac aggttgtatg tcaattatca cttctaataa attaaccgaa
     3781 tatgatgaat tcgccatcaa acattggggt gaaggtggca ataacaacgg cacgattact
     3841 ggtgaatgga cgaatccatt ccctggcagc agtttagata aaagttcctt cagcggtggt
     3901 caattatttg gaacgaatcc aggcggtgaa ttccgtccga acggcttcca tgatggatta
     3961 gattttggtt cagttgatca tccaggaagt gagattcatg cggttcatgg cggaaaagtc
     4021 gtgtacgttg gcaatcctgg tatttcaggt ttgggcgcgt gtgtgatcgt gattaattac
     4081 gatggcttga atatggtgta tcaagaattt gcaaatagta caggaaattc tcgagtgaaa
     4141 gtaggcgatc aagtgaaagt tggtcaagta attgggatac gagatacagc acacttgcat
     4201 ttaggattta ctcgaatgga ttggcgtcaa gcacaaggac atgcattcat tgatgatggt
     4261 acatggatcg atccgttacc atttttgaat agcagtaaaa aatagatcag gagaaaaaat
     4321 atggaaaata aaacgttaaa aatcgtctta acagctgcaa ttagcgttag tttaatcggc
     4381 aatattgcgt tgttttggca gctgaaagag gaacaagcag caaataccta taaaattgaa
     4441 gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt cctctgaggc acaggcgaag
     4501 atcgacaagt taaatcaaac catcaatgag aaaaaccaag aaatcgatca gctgaagcaa
     4561 caagatggca caaaagcaaa agaggaagta actgaagcac aaagaaagac ggcagaagag
     4621 ttcggtcaat tggccattga taaatcatta agcagaaatg aattaacgga aaaactaaaa
     4681 gatgtcgcaa cccaagaagt gatcgacaaa ttaagtccta cagacgatga tcaccaacat
     4741 gatgattacg gaagttactc ctttacatta ggtgacgtgc aaacttacgc tcaaacaagc
     4801 agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt atacaatcgg caatccgcaa
     4861 ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa ctgtcactga aaaacaagta
     4921 gatggtcaat ggaaagtcat agactttagt tatttcactc gttaggagga agaaaatgag
     4981 taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa agttatctta gatggctggg
     5041 aatctttatg attctcggct ttttgattct tgctagtccg atttatacga atcagaaggt
     5101 agatatacca gatgaggtaa gcacgaacgc ctttttaaac acgaaagagc tgacaatggt
     5161 ttcaaaagaa aaaggagcga accagcaatt agtttttgaa tttgcgatcg atgatgaaga
     5221 tcaaagcttg ttaaaagaac tggctaatct taattatcgc gtagaagtaa agacagctaa
     5281 aggggattat caagcaatac gagccaaagt gattaaggtg tctgatgatt attttgcagt
     5341 gaagctaacg catgtaccag aagaatatat cgccatgcgt ttaaccatta ttcctgaaaa
     5401 aattgatcca aaagtggata tgcaagaacc acaagatctg atttattacg tgcacgaaga
     5461 taaggttaaa gatcaagtga aagatgaaga ttatgaacaa cacgcgatca attacaaagt
     5521 gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa atggaatctt tccaagcaac
     5581 cattgatttg aatgaagaat tagtgaagaa gctaaaagat caattgcctt atcaagtcgc
     5641 agatgatcag gaaaatactg aaaacaagat taatagctac cagcaagaaa ttgaaacatc
     5701 gaaaacgcaa ctgaaagatc aagaagagat cattcaaaaa cttcaagaaa aaattgatcg
     5761 tatgacaaaa gaaaagattt agaaggacag gtggagtgaa aaaatgaagc gaacaaactt
     5821 ggctgacaaa gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt
     5881 aaaagctaaa gaaatatgtc gagaagtgaa tttatctaac acaacaatat ctgagttgaa
     5941 gaatggtcga aagaatttag atactttaaa attcagatca tttgaagctc tatatgtagc
     6001 tgctaaaaaa tattttacag ctgataacaa ataaaaaaca ggagtatttt atgtgaaaaa
     6061 agtatataaa aaatgcttat gaatcagcta tatcacctta accatttgtt aatgagatgg
     6121 gagaaaacat taaccacgat tctaacgcaa acatcaagca cataaaacat ttaacactcc
     6181 tcctttctgg ctgaagattt caaccaaaca ggaagacagc gtatcaatta tagcatacac
     6241 tttttcttaa attcattttt catatgacaa aatatttcct tctaatcgac aaagttagaa
     6301 ggatttttta ttgtctaaat acacaaataa ccttaatttt taaagagcat aaggaaacgt
     6361 gatatcactt tctcacgaaa ggtgttaggt aggctgtgcc taccacgttt ctaagaaaga
     6421 taaatgatat caaaattttg gatttgtgtg tagatttgtg ggtaggatga aagcgaatgt
     6481 gggcagtaaa ataagcgttt gtgtgtagca aaatcaaaaa atgtgtaggg atatgtgtag
     6541 ctacacatat ccctacacat aataaatttg gaggaaaata tggacttatt aattagaaat
     6601 attgaagaaa agtat
//
LOCUS       DAXWLB010000143         6575 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXWLB010000143 DAXWLB010000000
VERSION     DAXWLB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.146"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(55..216)
                     /locus_tag="ACGE4H_002288"
     CDS             complement(55..216)
                     /locus_tag="ACGE4H_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFF8365677.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(213..515)
                     /locus_tag="ACGE4H_002289"
     CDS             complement(213..515)
                     /locus_tag="ACGE4H_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365678.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(508..672)
                     /locus_tag="ACGE4H_002290"
     CDS             complement(508..672)
                     /locus_tag="ACGE4H_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365679.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(677..946)
                     /locus_tag="ACGE4H_002291"
     CDS             complement(677..946)
                     /locus_tag="ACGE4H_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365680.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(958..1707)
                     /locus_tag="ACGE4H_002292"
     CDS             complement(958..1707)
                     /locus_tag="ACGE4H_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365681.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(1710..2396)
                     /locus_tag="ACGE4H_002293"
     CDS             complement(1710..2396)
                     /locus_tag="ACGE4H_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFF8365682.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(2402..3073)
                     /locus_tag="ACGE4H_002294"
     CDS             complement(2402..3073)
                     /locus_tag="ACGE4H_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFF8365683.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVISGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(3066..3407)
                     /locus_tag="ACGE4H_002295"
     CDS             complement(3066..3407)
                     /locus_tag="ACGE4H_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365684.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(3585..4283)
                     /locus_tag="ACGE4H_002296"
     CDS             complement(3585..4283)
                     /locus_tag="ACGE4H_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFF8365685.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4370..4840
                     /locus_tag="ACGE4H_002297"
     CDS             4370..4840
                     /locus_tag="ACGE4H_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365686.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(4883..5062)
                     /locus_tag="ACGE4H_002298"
     CDS             complement(4883..5062)
                     /locus_tag="ACGE4H_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365687.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(5049..5387)
                     /locus_tag="ACGE4H_002299"
     CDS             complement(5049..5387)
                     /locus_tag="ACGE4H_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFF8365688.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(5403..5606)
                     /locus_tag="ACGE4H_002300"
     CDS             complement(5403..5606)
                     /locus_tag="ACGE4H_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365689.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(5621..6367)
                     /locus_tag="ACGE4H_002301"
     CDS             complement(5621..6367)
                     /locus_tag="ACGE4H_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFF8365690.1"
                     /translation="MNTPQIFSFEQHEVRTFLENDIPYFVANDVAKTLGYKNPSKATN
                     DHCKKSIETWGNDSLGRRQKFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT         1732 a         1358 c         1036 g         2449 t
ORIGIN      
        1 attctgactt ggttaattca gcaattcttt tcattgctgc tttcttatcc acgctcattc
       61 ctccaatcga tggatttccc ttcttaaatt ctctatgtgc aaatcgattg cctttctcgc
      121 cgtttcattg accatcactg cctttgttcg ttccagatcg tcaatctcac gctgaaggct
      181 tcgaatacgc atttgaatca cttcttctgt tgtcatgatg attcctccac gtatctaaac
      241 gttctcttct tagcgtctgt gtagccacac cttgctcttt tcctcacgat tttctcgtgc
      301 aagcctgtga gacttgctaa ctgttcagct gtccctatca ctagaagttt gtcaccgtgc
      361 caaattgcga tttttcgtgg tcttggcttg ttgctcttgt ctgcccacat cgctcttcca
      421 agcctcatca cttctgaagc agcttctttg tcattttgcc aatcttctga ataagtcaat
      481 tcgataattc gctgcattgc cgctttctta tccatcccga cattctcctt tcagtaattt
      541 gagtacttga tcaagtgcgc tctcacgtcc gccatggaac gtgttgagcc acttgtcttc
      601 gtacgataca ctttgtctta aagcttcttg atgcattagt tcaatctgtg ctgtaaatgt
      661 ctttaggtcc atctgattac acctgctcaa gttcattaag atgtttttgc aagcctttaa
      721 cacaatcaac aaatagtaat ttggtatatg ctaaatttct taattgtgtt gtatcgatat
      781 aaagtgcgaa atagtatctg agcttactcc aactcgaacg atcattctta attccttcaa
      841 ttccagcttc ttcgagttga tcatatacgt ctctcaggac gtctaattcc tcgccctttt
      901 tataattcgc tatttcatta attagttcta gataatcgat cttcattttt ccacctctta
      961 gaatggtgct tttgattgtc tattagctcg ttctagcgct tttttcttga gataggcttc
     1021 ttggtcgatt gcccattcag gaagcttctc tcgtcttcct gtgcgcttgt atccactgct
     1081 tgcgttctta ggttcacttt tttctttcct tgcccaactt cgaatagttg ccaaatagtt
     1141 tttataagtc ttaccagatg attcacaata ctctgacagt cgttcgattc gctcttggta
     1201 atcattaggg aattctgttt tgagtttctc catctgctca tctgacaaaa gaacattttt
     1261 atactctccg tatttatgac ggacgggctt agccttcgat tttttcgaag gcggtaactc
     1321 tcttatatat tcttttgtat tattaaatgt attattaata gatgtattat tatctttgac
     1381 tttttcgtca ataggggtat tgcgtttttc gtcaataggg tattgattaa ttcgtaggta
     1441 cctattgatt atttgattgg taccctcttt gtaaatgatt tcccgattca agtatccaaa
     1501 cttaatcaaa tcacttaccc atcgcgatat ggtctcttta ttcacaccat ataaatctgc
     1561 aaagtactca ttgcctgccc aacaaaagcc tctttcatta cacaaggccg ttatctctcc
     1621 gtataacaac ttagtatttg gtttaagtct tttgtcgtac cttacgttgg ctggtataat
     1681 cgcataataa cttcgatgtt ctgtcatttt taccctccaa tatttaactt tttgattgtt
     1741 ttctggttta atttgatccc tttgatttga tatttatttt tgaaattaat cacacctatt
     1801 ttgtgcttct ctgtgtgatg gattctgcag agtgctgcaa atgtgtactc tgcatgatca
     1861 acttctttgc gctttcgtct tcctagcgct ttgtcaaagt gatcgatgtc agctcctgtt
     1921 ttgccacaga tgcagcagac tctttttgtg atgcatttgt agaagtaata ttcttgattc
     1981 gcaggtaaaa tctcatagcc ttctttgaaa ggaatatgat gttcaaagat gaaatctaag
     2041 atgatatttg ctaagacatt ggcatcactc acagttgtat tcgattcgtc tttcaggctt
     2101 attttgcgcc ctgtgacacc ttcaaaacga aagtagaaga attccttcca gaagtccgtt
     2161 ggcatgcctg tatcgataaa aatatcgcct atcagcgcat agatgaagtt tcgttgctgt
     2221 acggtgaaac gtctaggatc aataaaacga atttcaatga ctcgatcacc atcgtagccg
     2281 tcatacatcg tctttagtcg atcaatgttc acttcctcat tgatggttgc gcttatgtct
     2341 tttcctttga actttttcag aaccgctgaa tatgaatcga ttaatggttt aaacactcat
     2401 atcacttctt atctaattct tttctcttag ctgctattgc tcgctccatc aaggcacatt
     2461 gctcatagct taactgttca atagtttcaa cgttatcagc taagagccct aatttatctg
     2521 tctgctcatt aacatattcg attaaggttt tggtcatatc tttacccatc tgctcattga
     2581 aagcttctag aatcgtctct agcatgttta atttctttgt atcgattcta ggtggtgttg
     2641 gaatatcttc cccttgaaat acatataatc ccagtccgtg tagagccaat gctttcacaa
     2701 agcatcgctt caatgagtta ttgatttgca ttgcatttgg tttaacaact ggttggtttc
     2761 gataatctaa aacaggaaat aattcggttt ccgtgtgtcc tttaaccgtt actgagacag
     2821 atacataagt cccagtttca tccataagaa aaggtttata ttcctcaaca agaaagtctt
     2881 gatgagttcc agaaataacc ctgtagtgtt tgtactcatt aatagttacc gttgcctgtg
     2941 gatcattctt tttcataatc tcccacgcgt gagcccaaga taaataatca aaatttcctt
     3001 ttttcttgag aattttattt aacttacgac taaaaagttt ttcaaagttc gttgtccctt
     3061 tgatttcact catcaaattc tgcctccatt tcagcaatgt atttcttacc tggtccgtaa
     3121 taagagatat caatcaagtt atctctgtcg tactcttcta gcgcatcaat caagccatct
     3181 tcgatgacgt aaatatattc aggtttattc gaatgcttcg atagatggat aagataaaca
     3241 tgatcccaaa tagttacatg gttgcctaaa tcatcttgat cccaagctag ttcttcattc
     3301 gtcaaaagat ttcgtctgat ttttcgacca cttgtttcct caattttcgg cttgccccaa
     3361 tcaggatcaa tcaaatattg atctagagtg gaaagttctt tttccatatg ctaaaatctc
     3421 cttatgatgt gttttctttg tgactctttg cttgccggcg gagtcacttt tttatttgtt
     3481 gccatgcttt ttgcttgtca atatgttgtt ggcttaggat gattggttta tagtatttcc
     3541 accagcaatt agcaattgcc gtccctattc ttagcgcttc agctctattc attgtcatca
     3601 ccaaaaagtc tctgttgtct gttcagttga tcaatttcca tacggatcgc agtttctggc
     3661 aaccacattt caataaatga aacagcatca tcgaatctct tacgaggtaa ctcgccatat
     3721 cttgggattg aaaaggtacg tttaaattca gaccaaaatt ttgagaatac ttttttgctg
     3781 atttcttcat aagctcggct ttcttttccc cctagaactc ccataacttt catatttcct
     3841 ttttgcttaa tttcaaactc ttgttgtccg ctaattcgca tagtatcttt aagcatggaa
     3901 acatcttttt taacatcttt catttcttct aattgataga tcatcatatc ttcaattgtt
     3961 tgaggaacag tattcttgcg aataacatct tccatttcgt tgaatgcttc aatgtatttt
     4021 tgtttgaagt agatagcttt ctttcctgta aaccccatag ccagcaagaa aaatccatct
     4081 ctactaatga agaaaactcg tcgatttctg ccatatgaat ctggttcatt accttctaca
     4141 aacatctgtc caaaattgga cacatctttt tttagtgcat caatatctct taaaacatgt
     4201 tgatgttttt tctcgaagct ttctgccact tgtaagctcg tagtcacagc ttctttattt
     4261 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat ttagttaaaa
     4321 actggtggtg tttattttga atttttttca cttaacaatt tttcaatgga tgtcaataat
     4381 aagttttata ttgagtacgt ttttaacagg gataaaaata aaaggaatgc gccctcaact
     4441 agaagttgaa cttaatgctt cttacttcgc tgcagatatg atttatacaa aagtaatcat
     4501 ttctaactat tcaacagaac cagctatgtt agttaattta gaactttcat ccgctaactt
     4561 aaaccgcaga tggtcagcta ctccatttaa gaaattaatt gctaaaggag gatcagtcga
     4621 tgataataga atatactctg aagctgtacc gctaaatatt cctcctaaaa gtgctatatc
     4681 ttgttacctt gcatttgaag tagggaaaat taattttaat aaactcctta atcaccaaac
     4741 aaaaatgata tttactctaa atagaaccca aatccacaaa attgttgata tcaaaaatac
     4801 aaacttccca atagagaaat tagtgaaaga attaaactga gtaatagtaa tatacattct
     4861 agtttctctt ttctattcat ttttagtcag ccccctcggt tggctttttc gctctgtact
     4921 cagcttcagc ttcatcaagc cccataaaaa tccaaaccat gtaaacaatc gttcctatca
     4981 acgcttgcct gcttccccaa agtcctaaag cgtagatgat tagcggtgcg ctgaacacta
     5041 gcgctctatt aaattttccc atccgcttgc ctccttaaga tttccgaaaa atttgtttct
     5101 aaaaattcaa gtgttttact tcttaaaaat agatatgtgt ctcttccttc aactggataa
     5161 tagacaaatc catttttgtt tttctcgata tcgataatat ttctatatct tgggttcttt
     5221 aaaactctag aagtaaacca atcatatttt ctgttaatcc gttctagcac ttctggcaac
     5281 gtcatccatc taccagtatc atcagctttt tttaactcct cataatccac ttgggagata
     5341 attacatagc cttctggaat tgggattttc gcttctagat attgcattag ctgttcctcc
     5401 ctttacaatt cgtacatagt aataatcgaa tctatgattc tatttgcttc tgcagaagtc
     5461 tttttaccgt ttaaaattaa agataagtag cttttgctaa ttccaaatct ttcagcaagc
     5521 atggtgtaag ttaagaactt cgaactttcg acatattctt tgattttttc tctatctcgt
     5581 tgagtgattt ctgcaatgtc agtcatacta aaactccttt ctaaccaatt tcctctaaat
     5641 ccatttgagg gtaatagccc tctgcgatta ataagttgta aataaacact cgccctttct
     5701 gtgtccattt ggtattcatt acaactttag tgccgccatc ggctttcgga atctcacttg
     5761 tgtgcgattt tgtgtatcct tggttcatat gttttcggta aagtatccat tgtccgctaa
     5821 ctttatgttg aacacctaac tcgtttagca atttgtttag agcaattgcc gacattccgt
     5881 aatcagctgc aatttgagaa gtagctaccg tatctgtcga tgaaagaatc gtatctaagt
     5941 agctaatctt tggttcgtac tcggcaattt gttgttcgag taattggttc ttttcttcta
     6001 aatctgctgc taaacgcaat gcttgtgcaa aactttgagg tacgtttgaa tagctacctg
     6061 tttttctgat tgttggaagg acttcttcca ttacccaggc ctcaaatttt tcagcgcttg
     6121 gtaagttcga tttaataatc aagcggtaaa catctgattc tggaataact ttgaattttt
     6181 gacgacgacc taacgaatcg ttaccccatg tttcaattga ttttttacaa tgatcattgg
     6241 tggctttact gggatttttg tatcccaaag ttttggcaac atcgtttgct acgaaatacg
     6301 gaatgtcatt ttctagaaac gttcttactt catgttgttc aaaactaaaa atttgtggtg
     6361 tgttcatttt tgttcattcc tttctttgat ataattttct tatcagtaag tggtctactg
     6421 aaataatgca taaggtggtg aataagtatg aataaaaaaa ctgatgaaat cgctgcggat
     6481 ttagttgttg catggctaaa tcacgaatca caagtatcta ctaaaggcga atcaatttcg
     6541 ccaaaagaaa ttgctcaagc ttatttagat attca
//
LOCUS       DAXWLB010000144         6557 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXWLB010000144 DAXWLB010000000
VERSION     DAXWLB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.147"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            113..709
                     /locus_tag="ACGE4H_002302"
     CDS             113..709
                     /locus_tag="ACGE4H_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFF8365691.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            722..1621
                     /locus_tag="ACGE4H_002303"
     CDS             722..1621
                     /locus_tag="ACGE4H_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFF8365692.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1624..2109
                     /gene="ssb"
                     /locus_tag="ACGE4H_002304"
                     /pseudo
     CDS             1624..2109
                     /gene="ssb"
                     /locus_tag="ACGE4H_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2131..2388
                     /locus_tag="ACGE4H_002305"
     CDS             2131..2388
                     /locus_tag="ACGE4H_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365693.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2547..2669)
                     /locus_tag="ACGE4H_002306"
     CDS             complement(2547..2669)
                     /locus_tag="ACGE4H_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFF8365694.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2824..3083
                     /locus_tag="ACGE4H_002307"
                     /pseudo
     CDS             2824..3083
                     /locus_tag="ACGE4H_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3531..3737
                     /locus_tag="ACGE4H_002308"
     CDS             3531..3737
                     /locus_tag="ACGE4H_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFF8365695.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3737..3988
                     /locus_tag="ACGE4H_002309"
     CDS             3737..3988
                     /locus_tag="ACGE4H_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365696.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4004..4441
                     /locus_tag="ACGE4H_002310"
     CDS             4004..4441
                     /locus_tag="ACGE4H_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365697.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4434..5129
                     /locus_tag="ACGE4H_002311"
     CDS             4434..5129
                     /locus_tag="ACGE4H_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365698.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            5294..5494
                     /locus_tag="ACGE4H_002312"
     CDS             5294..5494
                     /locus_tag="ACGE4H_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365699.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            5510..5689
                     /locus_tag="ACGE4H_002313"
                     /pseudo
     CDS             5510..5689
                     /locus_tag="ACGE4H_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5751..5935
                     /locus_tag="ACGE4H_002314"
                     /pseudo
     CDS             5751..5935
                     /locus_tag="ACGE4H_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            5969..6400
                     /locus_tag="ACGE4H_002315"
     CDS             5969..6400
                     /locus_tag="ACGE4H_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFF8365700.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2333 a         1085 c         1338 g         1801 t
ORIGIN      
        1 ggattcaaaa agaaatcgaa ttagatcgat aaaatcttcc taactattgt atacgtataa
       61 acttatgtta tactgttttt ataagtttat acgtatacaa aggagggaga aaatgacaaa
      121 agcaatggta tacaagctgc tagaagaatt taatggaact ttggctttga aggatgccaa
      181 aaaagcgggc atttctcctg taacgatcaa acgtatggta gatcgagggg agctggatcg
      241 agagtatcca ggttttttca ccttacctgg tcaatttcct gatgaattgt ttatggcgca
      301 aaaaaaatat gaacgcggca ttatttcaca tattaccgcg ttagatttgt atgatttaac
      361 agatatgatc cctagacaga tcgatctgac tgtaccttac ggttatcacg tttctgaaaa
      421 aggattgaag gaattcgcag ttgagctaca ctatacaaaa gcggaatggt atgaattagg
      481 taagattgaa ataaaaagtc gctatgggaa tcctattata gcttatgatc cagaaagaac
      541 tttatgtgac atttggaatc cctggtatac cgttgaggat gaaatcaaag taaaagcaat
      601 caaaaactat atggagtcgg acagaaaaaa tcttagaaaa ttgaatgagt atcgacgaat
      661 tttaccaaca gacaaaacga tgcgttctta tattatggct ttaaattaaa ggagaagata
      721 catgacacct acgcagttaa aagcaaaagt gaagaatatt tctagagaaa aagaagttga
      781 tccgcagctt gttttaagac attttatgat ggaaaaattc ttggaaaaaa tctcagagtc
      841 tccttatcgt gaaaattttg tgttaaaagg cggcttcttg attggctcaa agtatggcat
      901 tgaaaatcgc acaaccaaag atattgatac gactttacgg gaaatgaaag taaccaaaga
      961 aacattaaca acggttctaa acgaaatttt ttcaacgccg acgaaagaag gcattcaatt
     1021 tgagattcaa ggaatgaaag aaacgagaga agccgattac tatccaggat ttagcttacg
     1081 tgtgctggca catttggaaa acatgcggcc agattttaag gtagatgtga ctacaggtga
     1141 ctcgatctat cctgcaacga tcactcacag tcataaattg atgtttgagg atcgaacgat
     1201 tcctttggaa tcctatccta cagaacaaat tattgctgaa aaattaagtg caacctttga
     1261 ttttttaaca gataattcga gaggaaaaga tttttatgat ctctatacta ttcctaaaat
     1321 ggagaagatc gatgagaaga ccttgaaaga tagtgtgaga aatacattta cacgtcgagg
     1381 aaaaactgat ccgttaaaga tatactatga aaaggatatg gatgtactga aaaatagtca
     1441 cgagctaaaa gatacctgga agaaatatca aaaaagcaat ccttatgctg cgtcgatcac
     1501 gtacgatgag acaatggatt cgattagtaa tttgatggaa aaagtgattc acgtcgaaga
     1561 aaaagagcga aaactagctt atcaaagaca ccaacagatg aatagagaaa tggagagata
     1621 aagatgatta ataatgtgac attagtagga cgcttaacca aagatcctga tttacgttat
     1681 accgcaagtg gtactgcagt agcgactttt accctagccg tcaaccgaaa tttcacgaat
     1741 caaaacggca attgagaagc cgattttatt aactgcgtga tttggcgcaa accagcagaa
     1801 acaatggcga cattagctaa aaaaggtagc ttgattggtg tagttgggcg gattcaaacg
     1861 cggacttacg acaaccagca aggacaacgt gtatatgtga ctgaagttgt agcagataac
     1921 tttcaactat tagaaagtaa agcagcgaca gagagccgtg cgcacgctga tcaatcgtcc
     1981 accagtccaa gcaccgccac ctttgaacaa cggaatacag cgacgcctaa caacaatgga
     2041 ttgaacgctt cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta
     2101 ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aaattagtct gacgatggat
     2161 ctattgatac ccgtaattgc atttatagac gggatttctt tgggcgtcac tgaaaatcct
     2221 ttggtaaata gaattgcgaa aggatcacta tttcttgtat tcgtgtatct tgtctgttct
     2281 tatcttttaa aaagagcagg aaaggataat tccttttggc gattgactaa agtcacagta
     2341 gcagcgacag ctgttatact tacggctatt ctaccgcttt atatgtagag ttaacgggaa
     2401 atcgttgaac ttcttgcgaa atattgctat aatgaacatg aaaaagagga gttgcgctaa
     2461 caacccctca ttaacagcac cgattaaaga acggtggcag aatattagtt atttggaaat
     2521 aaccgctgac ctgctaaagt agacggttat ttctttttgt catttttaag catatttaca
     2581 atcaaactaa tcaaagcaat ggtaaacata ccaaaaccaa gaatggtttg aattgtctca
     2641 tatgcggaca aaaggcttac tcctttcata gattttagca cttacattca taagcaccac
     2701 cacctttcca gaagtcgcca ccgtcttcaa actttactgt cttgactatt atagcatatc
     2761 taaaatcgaa taaaaattct caattttagc tgtttttgct ataattttag cgaggtgatc
     2821 gtgatggaac aatatattgg tttactcgat aaattagggt gatcaagaca cagccattgc
     2881 ttgtgcgatt tacattacgc acgagtcatg aaccaatcaa ttgtgtagtg gccaatatag
     2941 aaatagcgga taaattgctg attttggaag aaggcaagta tactatttca gtgacaggtc
     3001 acttcaataa acgaaatcaa ctagtcattg aatcaatgca aattagaaac cctgatcatt
     3061 ttacgagatt aatgggcata taaataaaag aagagctgca gtcctaaatg aggattgcag
     3121 ctctttttgt tttacataga aacaagaaaa atgaaaaggc ctttttagga aggcgcaagc
     3181 gccttctcaa ttgaaaaacc tctctctcac tccaaccgcc accgccgttc gagaagaaag
     3241 gaagtgaacg ttgcacgttc actaagataa ggggtcttgc aaagcaagac aaaatagaaa
     3301 aaacttcctc tcaccaaaag gttcgttcct gttcgctatc tctttattga tttgccagac
     3361 cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa tggcacgctg
     3421 cacgcccttg tccttgtcgc ttttagttga ttcattctgg ccaacaaatc aataaagaaa
     3481 gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaatc atgcgaaaga
     3541 aatttgatta ttggggtgtt cccttcagcg aattattccc aaactatcat gcaccatata
     3601 ctgtcgaatg cgattgtgga gaaagagcga aatgtatcaa aagctattgt ttgtatcaat
     3661 gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttatattttg attgatgaga
     3721 aaggaaagaa acgctgatga aaacacaaca caaattattg actgtaaaag aagtcgttca
     3781 aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac ttattccgcg
     3841 gtactggacg atagaaaccc ctttagtaga ggatgcaccg actatgctat ttgaagacct
     3901 agtgaagatt gcaggatatg aactgcatta tcaagaaaac atggaaactt atgaaaacga
     3961 atattggtta accaaattag agaactaaaa aggagaaaaa aatatggctc aaaattacta
     4021 tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg atatgacatt
     4081 cctatacaat gagacacgtg ttccaaaaaa acattataag gaaagattgt cggtagctgt
     4141 ggaagaaatg attgaatcgg gtgtcgaaat gaatctcatt gcgacctact atcgaacgct
     4201 tgaagaactg aaaaagcaaa atggacaatg gtttttccaa gccttactat gcttggaagc
     4261 aggcgtcaag ccaaacacta tcaaaccttc tgaataccaa gctttagaac taacgtatgg
     4321 gaaatttgtc gaaacgaaaa aagccaagac tgtttcaagt gaatggttgg actacttaga
     4381 aaatgtcaac aagtatggcg catgttacat catgaaaaag gaggacaacg aaaatgaata
     4441 aggtgattta tcaactaaca acggaagatg tgtcacactt tttaacaaac aaagaacatt
     4501 tgaatgaagt aatcagacgg attcaaaaag actttagttg tatggaagaa atgaagtact
     4561 ttattcaaag agtcgaagaa caattgtatt tggaaagtga aacgagtcaa acgaaggtta
     4621 cagtcagcaa taattattta gacgtgtata aacgtcgtcc tgacacatta aaaaaaggag
     4681 cggagcttct acgactagaa acagaaaaat atatcatgaa tgtttaccta gaaaggagtg
     4741 aactggtcga aaactttgtc agctacacga tttgcaggaa acactattac tcagaatcag
     4801 aatattattc aatttttgag agcatggaat taatggaaga gctaaacagc aaagatgcga
     4861 cggtagacga ttattacctt gtatttacta aatttttgac gtgtgggacg gaatctatcg
     4921 tgttaatggg aatggattat gaactcgctg tacccgaagt atttttgagg gaatatgtag
     4981 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
     5041 acacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
     5101 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
     5161 ataaaaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
     5221 ccgcccccgc cgcttaggtg gtgcgaaata agcgagcaaa gcccgaaaac ctaagagaaa
     5281 gaaggagatt tatatgaaca aaattattcc cttattatta gtcctatttg ttggagaaat
     5341 acttatgcga cttagtgcag ctgtacattt aaacctcgca gtatttttgg taatactaat
     5401 gttttgtttg atcttttggc gatttgtatt agccctcgta agtctactat tcaagatgat
     5461 tgcagccata tttttcatag gattatttcg atgaaaggag agagtaccaa tgccaattgc
     5521 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagta
     5581 accaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
     5641 gattgcgcaa gaaaaagcag gatttgacat accagcactc aaaaagtgat cgcactttgc
     5701 gcacgtgtta atacgtgtta taattatttt gtaaggaggt atagtgcaca atgccactta
     5761 cgggaaaaga aatgcttaag ttattgaaaa aaatggctgg gttgagcgaa ggcaagaggg
     5821 atttcatcat catctttata aggatggtgt tagaatcact gttcctgttc atgccaatca
     5881 agatcttggc cgaggacttg agcgaaaaat tttaaaggat gcgggattga aataatctcg
     5941 cattcgatta aagaaaggat tgactgaaat gttaagtgag cctttagcaa aaacctatcc
     6001 tgctattttt agcccagaag aaggtggtgg gtactttatt gaatttcctg atgttcaagg
     6061 tgcttacaca ggaattaacg aagacgatat atcttatgga attgctatgg cacaagaggt
     6121 gttaggtatg gttttagcag attatattga gcatgaagat ctacttccag aaccaacacc
     6181 aatcaataag atttctgttg aagatgattc ttttactact ttaattcgtg tagatgttgc
     6241 caaatatttg aaagacacag aacttgtcaa aaaaacctta acaattcctc aatgggcgga
     6301 taagctaggc aaaagggctg gcataaattt ttcagtcctt ctaacagaat caattgcaga
     6361 taaagcagat aacattcttc gttctggaag aaataattag aactcaaaag ggtcagaaac
     6421 ttaaaaatct ttggcttctt tttttactac aaagatggct tctttaaatt atttgcctga
     6481 ttgatttaaa aaaattgcgc aaagactacg agctaattaa gttgctcgtg gtcttttttt
     6541 atgaaaactt tttagat
//
LOCUS       DAXWLB010000145         6486 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXWLB010000145 DAXWLB010000000
VERSION     DAXWLB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.148"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(3..2060)
                     /locus_tag="ACGE4H_002316"
     CDS             complement(3..2060)
                     /locus_tag="ACGE4H_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFF8365701.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2174..2386)
                     /locus_tag="ACGE4H_002317"
     CDS             complement(2174..2386)
                     /locus_tag="ACGE4H_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365702.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2573..3040
                     /locus_tag="ACGE4H_002318"
     CDS             2573..3040
                     /locus_tag="ACGE4H_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365703.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3037..3279
                     /locus_tag="ACGE4H_002319"
     CDS             3037..3279
                     /locus_tag="ACGE4H_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFF8365704.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3297..3599
                     /locus_tag="ACGE4H_002320"
     CDS             3297..3599
                     /locus_tag="ACGE4H_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365705.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3671..5131
                     /locus_tag="ACGE4H_002321"
                     /pseudo
     CDS             3671..5131
                     /locus_tag="ACGE4H_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5238..6317
                     /locus_tag="ACGE4H_002322"
     CDS             5238..6317
                     /locus_tag="ACGE4H_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8365706.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT         2101 a         1155 c         1362 g         1868 t
ORIGIN      
        1 ttttatttga agattggatt cgcactaata aatacattat ctacgccaat tctaccagca
       61 gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata aagtaaattt
      121 gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc atcgtctgat
      181 aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc accgtagcag
      241 cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg actgaagctg
      301 acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt gaattgcatt
      361 ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt ttgacgcata
      421 aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt tttattttga
      481 tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg atcgtcattt
      541 ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa gctatcccct
      601 tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt tacatgatta
      661 acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc agtcacattg
      721 tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc ttgaatagaa
      781 ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc tttcaaaata
      841 ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg atgcacttga
      901 ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt atcattacca
      961 atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc ttttgcctta
     1021 gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac ttgacctgtt
     1081 tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg tttgtgttgc
     1141 aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt ttgagaaacc
     1201 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     1261 tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca
     1321 gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga atctaattta
     1381 tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac tttcttagca
     1441 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt
     1501 cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg tttagaggtg
     1561 tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt
     1621 acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg
     1681 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag
     1741 ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
     1801 agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc ttcccaggcg
     1861 gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt tttgctagag
     1921 gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt agttgaagca
     1981 gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc taaaacagtt
     2041 tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg tttacatgat
     2101 tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt atataatttt
     2161 ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt
     2221 tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa
     2281 ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac
     2341 cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc
     2401 ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt
     2461 tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt
     2521 gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa
     2581 aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc
     2641 aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg
     2701 gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga
     2761 atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga
     2821 ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa
     2881 caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat
     2941 ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc
     3001 agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct ttttatttat
     3061 cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc cgcgtctaaa
     3121 atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct tgaaaaagca
     3181 caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta tcaacgtgaa
     3241 cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat aatcttatgt
     3301 ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca gttgatattt
     3361 ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg
     3421 ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag
     3481 ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg
     3541 ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa
     3601 aaaaacaagt aggaggagta aaaagtacgt ggctactttt tactcctttt tttatgaaag
     3661 gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag agttaataag
     3721 aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc
     3781 tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac aagagaatca
     3841 atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg
     3901 cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt atcctacatt
     3961 gatagattta atccgccttg atgtgtttaa agatacggat gaaactatga gtgtggaaga
     4021 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     4081 aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     4141 ctttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     4201 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     4261 tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg atccagttga
     4321 agctaatgaa gagtcaacat ggactgaagg ggattttcag gaatatattg aaccacttat
     4381 cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat ttgattatat
     4441 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     4501 tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag agagtaaaga
     4561 agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg tccaatcaac
     4621 aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac gtcaacaaga
     4681 aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg agaaagtaaa
     4741 agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa aagtcaaaga
     4801 gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt gtagaaaaga tcaacaattt
     4861 tactaagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa aagaacgtat
     4921 taaagaacga aaagaagagt tctaccgcgg tttacgtaca gataagctaa agattagcaa
     4981 agaattaaat gaacaactga ataagctata tcaaacaacg actgaagttc ttatggaaaa
     5041 acacgaacag atccagcaag aattacaagt acaaattcca aaatggaaaa aagagtatga
     5101 aattcaacaa caagaattga aagaacaaga aggcggattt tataattaac ttagccattt
     5161 ggaaataaaa aaacgccacg tgaattccat gttatattga agttaccaca actctcaata
     5221 gacaaggatg aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac
     5281 acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc
     5341 gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg
     5401 ggaccgttac tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg
     5461 acgtctacga cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca
     5521 gacggccctt atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt
     5581 tggaaaaatg gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc
     5641 cggccattat ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa
     5701 cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca
     5761 accgcccgaa taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta
     5821 cccggaaaac cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg
     5881 atgccgtgct gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg
     5941 ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg
     6001 cgtacttcgg ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac
     6061 tgcacgatgc cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg
     6121 agcgcgggac gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc
     6181 caatcggcaa cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc
     6241 cgcgaaagat tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg
     6301 aacggttgac ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg
     6361 tctggaaagc tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa
     6421 gggcccactc gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatgg
     6481 gtggct
//
LOCUS       DAXWLB010000146         6467 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXWLB010000146 DAXWLB010000000
VERSION     DAXWLB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.149"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(111..566)
                     /locus_tag="ACGE4H_002323"
     CDS             complement(111..566)
                     /locus_tag="ACGE4H_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365707.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACGE4H_002324"
     CDS             complement(720..1928)
                     /locus_tag="ACGE4H_002324"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFF8365708.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACGE4H_002325"
     CDS             complement(2040..2372)
                     /locus_tag="ACGE4H_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFF8365709.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACGE4H_002326"
     CDS             2520..3395
                     /locus_tag="ACGE4H_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8365710.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACGE4H_002327"
     CDS             complement(3477..4058)
                     /locus_tag="ACGE4H_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFF8365711.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACGE4H_002328"
     CDS             complement(4064..5323)
                     /locus_tag="ACGE4H_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFF8365712.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACGE4H_002329"
     CDS             complement(5325..6371)
                     /locus_tag="ACGE4H_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365713.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1804 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataa
//
LOCUS       DAXWLB010000147         6456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXWLB010000147 DAXWLB010000000
VERSION     DAXWLB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.150"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            4..741
                     /locus_tag="ACGE4H_002330"
     CDS             4..741
                     /locus_tag="ACGE4H_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8365714.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            755..1570
                     /locus_tag="ACGE4H_002331"
     CDS             755..1570
                     /locus_tag="ACGE4H_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFF8365715.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1583..2230
                     /locus_tag="ACGE4H_002332"
     CDS             1583..2230
                     /locus_tag="ACGE4H_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8365716.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2246..2902
                     /locus_tag="ACGE4H_002333"
     CDS             2246..2902
                     /locus_tag="ACGE4H_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFF8365717.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3064..3885
                     /gene="racE"
                     /locus_tag="ACGE4H_002334"
     CDS             3064..3885
                     /gene="racE"
                     /locus_tag="ACGE4H_002334"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFF8365718.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3888..5243
                     /gene="rph"
                     /locus_tag="ACGE4H_002335"
     CDS             3888..5243
                     /gene="rph"
                     /locus_tag="ACGE4H_002335"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFF8365719.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5244..5762
                     /locus_tag="ACGE4H_002336"
     CDS             5244..5762
                     /locus_tag="ACGE4H_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFF8365720.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5869..6369
                     /gene="cbpB"
                     /locus_tag="ACGE4H_002337"
     CDS             5869..6369
                     /gene="cbpB"
                     /locus_tag="ACGE4H_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFF8365721.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2116 a         1089 c         1430 g         1821 t
ORIGIN      
        1 tctatggcaa tgattaaatt tgatcacgtg gataaatatt atggcaagtt ccacgcatta
       61 aaaaatatca atctcgaatt cgagaaagga gaagttgtcg ttgtcattgg tccttctggt
      121 tcaggaaaga gcacgatgct tcgctgtatc aatggattag agacgatctc ttcaggtaag
      181 ctgctgatta atgatactga cttgcatgat aaaaagacaa aactgacaga agtccgaaaa
      241 aacatcggca tggttttcca acatttcaat ttgtacccaa ataaaactgt tctggaaaac
      301 atcaaattag caccaatcaa agtgttgaag caagaccagg caactgcagt caaaaacgcg
      361 gagaaatttt tgaaaactgt caatatgtta gataagaaag attcttatcc ttccatgtta
      421 tccgggggac aacagcaacg ggtggcaatc gccagaggtt tagcgatgaa tcctgagatg
      481 ctgctgtttg atgaacctac ttctgctttg gatccggaaa tgattggtga tgtattagac
      541 gttatgaaaa aattggctca tgacggtatg tcaatgattg tcgtgacaca tgaaatgggc
      601 tttgccaaag aagtggcgga tcgtgttatc ttcatggctg atggagaagt attggaagac
      661 agcagaaatg tgagagactt tttcgaaaat ccaaatgaag tccgagcaca gcaatttatc
      721 agtaaagtta tcaatcattg ataggaggaa aaagatgcgc aggttaaaaa aaatacgcct
      781 gattttcctt ttaggcattg cttttcttgc actagccgga tgtaaaggaa atgggttggc
      841 aaatgaagat attttagagc gaagcaaatc taccaatgaa atcatctggg gtgtcaagta
      901 tgatactcga ttatttggaa tgatggatat tgagagcaga actgtacaag gatttgatgt
      961 agatattgca aaagcaatca ctaaaaagat ccttggtgac aatggaaaaa cagaatttgt
     1021 tgaagtcact tctaaaactc ggattccatt attgaaaaac ggaaatatcg atgcaattat
     1081 tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt gacttttcag atgtctattt
     1141 cgatgctggc caagctttgt tggtaaagaa aggaagccaa atcaaaagtg tagatgattt
     1201 gaatgcttct acaacggttc tagcagtcaa aggttctaca tcggctgcga atatccgaca
     1261 gcatgcacca gatgcaaaaa ttttagaatt agaaaactat gcagaagcat ttactgcttt
     1321 acaatctggt cagggagacg cgatgactac ggataatgcg attttgttag ggattgcaga
     1381 tgaaaatcca gagtatgagt tagttggtgg aacgtttact aatgagcctt acgggattgc
     1441 gatcaacaaa gggcaagaaa acttcttgaa agctgttaac caagcgctag aggaaatgca
     1501 tgcagatggc acatacgata aaatctatca aaaatggttt ccaaatgaaa ctgaaggaaa
     1561 agtagagtaa gggggagagg caatgagcga attgatacaa acttatggac caatctttgt
     1621 cgacgggttc aagatcacat tgttttcgag tgtcttagca cttgtgttta gtttgattat
     1681 cggaacgtta atggcaatct tccaattatc aaagaataaa gtagtgagcg ccttagcaaa
     1741 ggcatatgta gaatttttta gaaacattcc attattaatc atcgtgatgt ttttttatgt
     1801 tgtcgtaccg atttattggt ttaatctaga tggattccaa gcaggtacga ttggattaac
     1861 gatctataca tctgctttta ttgctgaaac agtacgttca gggattcaaa ccgtaccaaa
     1921 aggtcaaaca gaagcaggac tatcttctgg ttttacctat tcagaaatca tgcgttatat
     1981 tgttttaccg caagcattca aaatcgttat cccgccatta ggcaaccaat ttatcaacct
     2041 cgtaaaaaac tcatctattt tagcaatcgt agctggttta gatttgatgt atcaaggaga
     2101 cttgatcgct agtgaaacat tcaatacctt cgatacgtat atcatcgttg ggttatttta
     2161 cttagttatc acgctaccgt tatcttactt aatggtttat cttgagaaaa aatgggcggt
     2221 tcgttcatag aaggagggga aatacatgga ttttagcggc gctttttcat ggatgaatat
     2281 ccgtttttta ttggaaggat taaaggttac tgttgaagtt tcattgtttt cgatcatttt
     2341 tagtttcttg atcggtggtc tgacaggtgt gattcgtttt gcgaatatcc ctttcttttc
     2401 caaaatcctt ggtgtagtga ttgatattat acggaatctt ccattattat tgatcatttt
     2461 cttcacttac tttgccttac ctcaaatcgg cattcagatg aacatcttct ggtccgctgt
     2521 cgcagcatta acgatttttg agtcagctat gttgtcggaa attttccgag ctggactcaa
     2581 tgctgttcca aaaggacaga tggaagcagg attatcaacg ggactgactt atgtagaatc
     2641 gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg atcccggcaa ttgtgagcca
     2701 attgatttca ctgatcaaag atacttcatt agcagtaatc atttctttac ctgaactttc
     2761 tcatcaggca cgtattattt atggacagaa caccaattat gtgcttccga tgttcatcat
     2821 catgacactt atgtacttcg tggtatgtta cgcgctttct ctgctttccg gttacttgga
     2881 aaagagacgg tacagttatt aatagatgat tcttttagcg gttgtggcaa actaaagtca
     2941 caaccgcttt tcctctttcc attcgaaaaa aagagatttc gattgtttta agcaagtaag
     3001 agcacttgaa aattcacgct aaaaagtaca caatagggag gatagaattt attgaaaaaa
     3061 aggatgatac gattgacaga taatcgccct atcggattta ttgattcagg tgtcggcggc
     3121 ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg aaaatatttt atttgtagga
     3181 gacacagcac gctgcccata tggccctaga cccgcggaac aggtaatcca gtatacttgg
     3241 gaaatgacgg attatctggt ggagcaagga atcaagatgc tggtgatcgc ctgcaatacc
     3301 gcaactgcgg tggctttaga agaaatcaaa gctgctcttt ctattccagt catcggtgtg
     3361 atccttcccg gtactagagc ggcagtaaaa aaaacacaaa ataaacaagt tggcattatc
     3421 ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag cactgaaaga gaaagtacca
     3481 gaattgactg tgacaagtct tgcttgtccc aaatttgttt cagttgtcga aagtaatgaa
     3541 taccattcat cggtggcgaa aaaaattgtg gcagaaacat tagctccttt aaccactaaa
     3601 aaaatcgata cattgatttt gggatgcacc cattatccat tattacgccc catcattcaa
     3661 aatgtaatgg gagaaaatgt tcaactgatc gattctggag cagaaacagt aggtgaagta
     3721 tctatgctgt tagattattt caatttgagc aattcaccgc aaaatggtcg gacattatgc
     3781 cagttttata caactggctc tgccaaactt ttcgaggaaa tagctgaaga ctggcttgga
     3841 atcggacact taaatgtaga acatatcgaa ttgggaggaa aataaggatg cgtcatgacg
     3901 ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac aaatgttttc aaatatccag
     3961 aaggatcggt agtgatccgt tttggtgata caacagttat ttgttctgca acaatagaaa
     4021 attctgttcc cccttttttg agagaaacag gaacaggatg ggtgacagca gaatatagta
     4081 tgctgcctcg agcaacgagt acgagaaatc gtagagaaag cagcaaaggg aaactttctg
     4141 gtagaacaat ggaaatccag cgtcttattg gtcgttcctt gcgtgcagta gtagacttgg
     4201 aaaaattagg agaacgtagt atcatcgtag actgcgatgt gatccaagcg gatggcggta
     4261 ctagaacagc aagtatcact ggggcatttg tggcattgaa attagccatt gaaaaacttt
     4321 taagagaaaa agaattgtca gaagatccaa taaaagaaca tcttgctgca gttagcgtag
     4381 gaatcttacc agatgggact tgcgtcacag atttggatta ccaagaagat tccgcagcac
     4441 ttgtagacat gaatcttgta atgacagaat ctggaaaatt cgtggagatt caaggaactg
     4501 gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat gctctttttt ggaaaaaacg
     4561 cgattgaaga tttgatcaaa gagcaaaaac atgctttgct aacagagttt gctcaaaatg
     4621 atgagcggat agaagaaaca aaaacaatca tcattgcgac aagaaatcca ggaaaagcag
     4681 aagagtttcg aaatatgttc aaagaagcgg gatatcatgt aaaaacatta cttgactatc
     4741 ctgaattgcc agatgtggaa gaaactggca gcacatttga agaaaatgcc cgattaaaag
     4801 cagaaacgat tgcacaattg cttgatcagc cagtcttagc agatgattca ggtttgaaag
     4861 tggatgcttt aggcggtatg ccaggaatct attctgcacg ttttgcaggg gaacaaaaaa
     4921 gtgatgcggg gaataacgcc aaattattat atgaattgac agatgtgccg gatgaaaaaa
     4981 gaacagcaca attccattgt acgttagtat tcgcggcacc caaaaaagac agccttgtag
     5041 tagaagcgga atggcccggt cgagtagccc gtataccatc tggcgaaaat ggctttgggt
     5101 atgatccttt atttatccca gaaggtaaga aacaaacagc agcagaatta tcgagcgaag
     5161 aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa attgagtgcc gagtggaaac
     5221 aatggctgga aggagagaga tagatgcggt atttagtagt aagtgataat catggagaca
     5281 gagatatcct cgttgactta gtagatcgtt atcgttctga agtggatatc tttttccatt
     5341 gtggtgattc ggaattagaa gcttctgacc cattatggga tacattccaa gtggttcaag
     5401 gtaattgtga ttatggacca ggattcgaac agaaaaaagt gatccaaact ggtcaggata
     5461 cagtattcat gactcacggt catttatcga atgtccgatt tggtttgaca cagttagcaa
     5521 tcgaagctcg agctgcaact gccgatatgg tgttttttgg tcatacccat caaataggct
     5581 gtgagatgga tcagaaaatc ctgtttttga atcctggtag catttctcag cctagaggcc
     5641 cgatccaaat cccatcatat gctcttatcg aaagtttaga tgaccaaatt tctgttcaat
     5701 actataaccg tgcccatcag gcagttgaag agttggcctt ttctttttcg agagagcgct
     5761 gaataaaaaa actctaattt ttaataaaaa taagctttat tttatgaatt ttgcatccgg
     5821 tttcggtaaa atagtagtgt tgaatgaaat agaaatggag gccatcagat gattggacct
     5881 atagtaagag aaatgctgct ggaaaatcag gaaacgtttt tgattcctgc agaaaatgtg
     5941 gcaaatgtta tgtatcatca tccattgtcc catggtttac ttgtgttatc gaaggtcggt
     6001 tattccaaaa ttcctgtatt aggaaaagat gatcactttg taggactagt aagtttatca
     6061 gatgtggtca ataaaatgat tgacttgcaa acaatcagca tggacccgtt agaaggacta
     6121 acggttgctg atgtgacaga aacagatgtt ccgacaattg atgaaaattg ggaactagaa
     6181 gaagtactcc atttgttagt cgattcgcct tttttaccgg ttgtcacaga agatcggatt
     6241 ttcaaaggaa tcattacaag aaaagagctg cttaaagccg taaacttcat ggtgcatgaa
     6301 ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat taaaagaaca aatcgatatc
     6361 gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat aagtagaaaa aacagcgtac
     6421 cagttttgac tttatcttga aaaagaggct gggaca
//
LOCUS       DAXWLB010000148         6420 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXWLB010000148 DAXWLB010000000
VERSION     DAXWLB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.151"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            515..1204
                     /locus_tag="ACGE4H_002338"
     CDS             515..1204
                     /locus_tag="ACGE4H_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8365722.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1191..2399
                     /locus_tag="ACGE4H_002339"
     CDS             1191..2399
                     /locus_tag="ACGE4H_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFF8365723.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2693..3964
                     /gene="serS"
                     /locus_tag="ACGE4H_002340"
     CDS             2693..3964
                     /gene="serS"
                     /locus_tag="ACGE4H_002340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFF8365724.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4513..5997)
                     /gene="guaB"
                     /locus_tag="ACGE4H_002341"
     CDS             complement(4513..5997)
                     /gene="guaB"
                     /locus_tag="ACGE4H_002341"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFF8365725.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1961 a         1203 c         1237 g         2019 t
ORIGIN      
        1 ttttttaaaa gttttttcga tttcgttttt cgaaatcttc cagcaactca tttatcataa
       61 cttatcgttt gttatttgtc aagaactttt ttgaagtttt tttcgataga atcaagcttc
      121 gcaagtggtt cttttttaac gactcagtta tcttatcatg tcatttccaa catgtcaaca
      181 acttattttt tattatttaa taagttattg gtgttctttt gaacaaataa taatatatca
      241 acatcacatg tgaaagtcaa taagaaaatg cttatttatc aacatttcta gcttacatga
      301 tcattggtta gttctggtcc tttttcgttc gtctttataa ctccgagaga cgtaaaatcc
      361 ttttgttttc agtttaatgt tcatgttctt gtcttattca caccaactag atgtctcacc
      421 ttctaaaatt ttccttaaca aagtgctgct tttatctatt tctgcttttt tttgctagaa
      481 taatagtaac aatatgactc tggaggattt atttatgaaa attttgattg ctgacgatga
      541 taaagaaatc gttgaattat taagtatcta tgttcacaat gaggggtatg aagcagtaaa
      601 ggcttatgat gggaaagaag cattatctaa aatcagaaca atccctgata ttgaattact
      661 tattttagat atcatgatgc ctgagatgga tgggatgcaa gtagtgaaag agctacgaaa
      721 agaatcccaa ataccaatca ttatgctgac agcaaaaact actgatatgg ataagatcaa
      781 aggactggtc gctggtgcag atgattatgt gaccaagcca tttaatccgt tagaagtaat
      841 ggctagagta aagtctttat tacggcgtac aaaaatgcag attgccccag atcagcctga
      901 tgtattagaa gtaaatgcat taatcatcaa taaagactct catgaagtta aaacaatcga
      961 tgggaaagaa atccagttaa cagctttgga attcggtatc ctctacttgc ttgctagtca
     1021 tcctaatcga gtatttagcg cggatgaaat ttttgagcgt gtttggaaac aagaaagtat
     1081 tgtctctgct aaaactgtca tggttcacgt tagtcattta agagataaga ttgaagaagc
     1141 aactggtggt gaaaaagtca ttcaaaccgt ttggggagtg ggctataaaa ttgacgcaag
     1201 atagtaaacg agcaactgaa aaacgccgtc ggatcaattt gacttcaaaa gaaatcagcg
     1261 aattgttagc ggaaggaatc atcacgatta tccttttgct tctattaaat gtttctattc
     1321 tggttgttat cagttcggtg atcaacagtt caccttcttt gacgaatgcg atttgggatt
     1381 caaagaatat ttttgcagaa cgattgaaca ctgatttgtt ctggaacggc cggaatttta
     1441 ttattccctt tttctttcta ttagatattg gtgttttgta ttggcgcttg attcgaagat
     1501 atcgccaaat gcaattgcgg catatcatca gcgaacttca ctacatcgca aacggaaatt
     1561 atgatcatcg tatccctttt gaactgagtg gtgacttaag ccgagtggta acaagtatca
     1621 atggattggt ggatagtacc gtagcagcga ttgaagacga acgtaaaatc gaaaaatcaa
     1681 aagatgaatt gatcacgaat gtcagtcatg atatccgtac cccactcacg tccatcattg
     1741 gatacctagg actgatagaa gatggccagt atcactcaga agaagatctt ttaaagtata
     1801 cgcatactgc ttatatcaaa gcaaagcaga tgaaatcttt agtggacgac ttatttgaat
     1861 acactaaggt acgccagcct gctgtcccgg tgaatttttc tgcctttgac atgatccagt
     1921 tgatcgaaca gttagcagct gattttgagt tggaagcttc taaaaaaaat atccaaatac
     1981 ttgttcaatc aaaagtagac tcccttatca tggatggcga taccgaaaaa cttgtacgtg
     2041 tcttcaataa tcttttgacg aatgcgttga aatacggaaa aggagcaacg aagatcgtaa
     2101 tcgaagtaga acgtatcggt tcagaagttg ttgcaacagt caaaaataac ggagcaatga
     2161 tcccacaaca ggcaatcgat aatctattcg accgttttta tcgtgtagaa gaatctcgtt
     2221 cacaagcaac tggaggaact ggacttggtt tagcaatcgc tcaaagcatt gtggctttgc
     2281 acggcggata catttatgca aaatcagata agcaatggac ttctttcatc atgcaccttc
     2341 caatcaagaa aaatgaaaaa ttacctattg atacacagga agttattgac gaaagctgaa
     2401 atattcgcta ttcttattac aattattaga ataaatgaaa aacacagaac ccagtacact
     2461 ttattctttt tcagagagct gatggcaggt gtaaatcagc aaaggataat ttggaagttc
     2521 cagttcgttt acgtgccctt tcgaaataag aaaggtcggt tcaacagccg ttatgtgttc
     2581 aacaagtgca gctgacgctg aacttgggtg gtaccgcgaa tttcagactg attcgtccca
     2641 agaataatgg gatggatcag tcttttttta ttttcaaaaa ggaggaaatc atatgttaga
     2701 tgtaaaaatg atgcgacaaa atattgaaga agtacaagaa aaattgatga ctcggggcgt
     2761 aaaaaaagaa acattagctg aattcatgga actagatgaa aatcgtcgta agctactagt
     2821 caaaagtgaa gaattaaaaa aatatcgaaa tgatgtttct gctgagatcg cccaattgaa
     2881 gcgtgcaaaa gaagatgctt ctgctaaaat cgctgaaatg aaagaagtcg gtggcaacat
     2941 caaagcattg gatactgaaa ttgctgaagt ggatgaaaaa ttgaaacaaa ttgcaactac
     3001 tttgccaaac cttccccatg attctgtgcc agttggaaca gatgaagagg acaatgtaga
     3061 aatccgtcgt tggagcgaac cacgtacatt tgcttttgaa ccaaaaccac actgggaaat
     3121 tgcagaaaat cttgatattt tagattttga acgtggcgca aaagtagccg gtagccgttt
     3181 tgtctattac aaaggattag gtgctcgatt agaacgtgct gtctacaact tcatgcttga
     3241 tcagcatatt tacgaacacg gctatactga aatgatcact ccttatatcg tcaacagtaa
     3301 agcgatgttt ggtacgggac aatttccaaa attcaaagaa gatgttttcc aattggagaa
     3361 tactgatcta acattaatcc caacagctga agtaccatta actaactatt ataatgatga
     3421 aattttagat ggtgcagatt tacctattta tttcactgca ctaagccctg cttttcgttc
     3481 tgaagccgga agtgctggtc gggatacacg aggactgatt cgtcttcacc aattcaataa
     3541 agtagaaatg gtcaaatttt cggatgtaga gcattcttac gaagaattgg aaaaaatgac
     3601 agctgatgca gaagatatct tgaaaaaatt gcatttgcct tatcgtgtga tggccttatc
     3661 aactggagac atgggctttt ctgcagctaa aacttatgac ttagaagtat ggatacctgc
     3721 acaagatact tatcgtgaaa tcagttcttg ttctaactgt gaagatttcc aagcacgccg
     3781 cgcaatgatc cgttatcgcg ataatgatgg aaaaatccac tatactcata ctctgaatgg
     3841 ttcaggactt gcagttggcc gtacagtagc tgccattttg gaaaactacc aaaatgaaga
     3901 tggttctgtc acagttccag aagcgctcgt tccttatatg gggaatctga aagttattaa
     3961 ataaagaggc tgtgaaaaag ccgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
     4021 gctttccaca tttttgcata aattggactt aataccgaga aactggcttt tgaacaccat
     4081 ttatcgagag gttgtgaaat cagcgaatta acctgagtat tagagaaaaa aatggaaaaa
     4141 tagtccctca tattttaccg tttgttgaac taataccgag ggtcagctga ttgaacaccg
     4201 tttagtggag aggtcgtaag cctacccccc agctcctagt tacaaggaca aattcaaaaa
     4261 acagctcttc atattttatg aatgttgtac ttgtgattcg gaggtggcaa gggaacactg
     4321 tttattcaaa aagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac
     4381 gaaaattgct tttcaaattt ttgtgaattt cttcttattt ccgaaggagt tgcttctact
     4441 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
     4501 tctttaatgt ttttaagatt caactgaata gtttggtgct tcttttgtga tttgtacatc
     4561 atgagggtgt gattctttta atccactgcc gctcatttgg ataaattgcg cttcatcacg
     4621 taattgctgt agattggctg ccccaacata acccattcct gaacgcaaac cgccgatcat
     4681 ttggaaaata atatctgcaa cacttccttt ataagcaacc cgaccttcga ttccttctgg
     4741 tactaattta tttgcttcat tgacactacc ttggaagtaa cggtcttttg aacctttttc
     4801 catcgcgcct aatgagccca tacctcggta tgttttgaat cgacgtcctt ggaagatttc
     4861 gaattcacct ggtgattcat ctgtccctgc caacatactt cctagcatca ctgcatgtcc
     4921 gccggcagct aatgctttga cgatgtcacc agaatatttg atccctccgt ctgcaataat
     4981 cgctttgcca tattgacgag caactgatgc tgcatcataa atagctgtga gctgaggtac
     5041 acctacaccg gctaccacac gtgtcgtaca aatcgatcct ggtccgatac caacttttac
     5101 gacatctaca cctgcatcat ataaagcttt tgtcgcttca gccgtcgcaa cgtttcctgc
     5161 aatcaatgtt gcatctgcaa aagttgaacg gatttcttga atttttcgga tcacacctgc
     5221 actatgtcca tgagctgtat cgatgatgat tgcatctgcc cctgcttcta ataaagcatt
     5281 cgcacgctca aatgtatcgc ttgttacacc aacggctgct gctacaagca atcgaccatg
     5341 ttcatctttt gctgcatttg gaaattcgat cactttctcg atgtctttga tcgtgatcaa
     5401 tccgcttaaa cgaccttcgt tatctacgat tggtaatttt tcgattttat gtttttgcaa
     5461 aatcttttct gcatctttta gagaggtacc tactggggca gtcactaagt tgtcttttgt
     5521 catcacatca gcaatcggca tgtgataatc tgttacaaaa cgcatgtcgc ggtttgtaat
     5581 tatccctacc aattttctgt tttcaagtgt ttcaacgatt ggcacaccac tgatacgata
     5641 tttgctcatc aattcttctg cgtctgctac aagatgctgt ggcgttaaga agaacggatc
     5701 aatgattacg ccactttcag aacgtttgac ttttcttact tcatccgctt gctggctgat
     5761 cgtcatgttt ttatggatca cacctagtcc gccttgtctt gccatagcaa tggccatctt
     5821 gctgtcggta acagtatcca tacttgcact gatgattgga atattcaact taatattttt
     5881 agctaattgt acgctcatat ccacttcgtt tggtaacacg tgactttctg ctggtatcaa
     5941 taagacatca tcaaaagtaa atcctttttt cgtgaacttt gtttcccagt tggacatttt
     6001 tagtaccact cctctaattt agtttatcta ttaaaataat tgattttttc atttaagtca
     6061 atgatctact tggaatcaag ttagctttct catagaatac caatataaag cgtttacatc
     6121 tgtgtatatt aaaagagatt gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa
     6181 tcaaaaacag cttctcatgt ttttgaattg gttgggttta ttaccgcaga tgcaaacttt
     6241 tgaacaccgt ttagtggaga ggctgtgaaa aagctgctta gtttcgagta gtaagaaaaa
     6301 ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg agaaactggc
     6361 ttttgaacac cgtttatcgt aaaaagggac tgtgacaaga cttttgtcac agtcccttac
//
LOCUS       DAXWLB010000149         6406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXWLB010000149 DAXWLB010000000
VERSION     DAXWLB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.152"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(167..355)
                     /gene="rpmB"
                     /locus_tag="ACGE4H_002342"
     CDS             complement(167..355)
                     /gene="rpmB"
                     /locus_tag="ACGE4H_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFF8365726.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            625..987
                     /locus_tag="ACGE4H_002343"
     CDS             625..987
                     /locus_tag="ACGE4H_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8365727.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1039..2721
                     /locus_tag="ACGE4H_002344"
     CDS             1039..2721
                     /locus_tag="ACGE4H_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFF8365728.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2838..4874
                     /gene="recG"
                     /locus_tag="ACGE4H_002345"
     CDS             2838..4874
                     /gene="recG"
                     /locus_tag="ACGE4H_002345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFF8365729.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALTGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4923..5924
                     /gene="plsX"
                     /locus_tag="ACGE4H_002346"
     CDS             4923..5924
                     /gene="plsX"
                     /locus_tag="ACGE4H_002346"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFF8365730.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6046..6288
                     /gene="acpP"
                     /locus_tag="ACGE4H_002347"
     CDS             6046..6288
                     /gene="acpP"
                     /locus_tag="ACGE4H_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFF8365731.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2149 a         1110 c         1443 g         1704 t
ORIGIN      
        1 gagtctaatt ttcacaaaaa aataaaattt agaataaata acttcataat ttgttcgtat
       61 aacaaaaata aacgcaaaaa aagggtagga atcaaaagtc ccacccttaa aaatttttat
      121 taaataaaaa accgttcact atctgaacgg tccaagtatc attcaattaa acgcgttcaa
      181 cttttcctga tttcaaagca cgagttgaca cccaaacttt tttaggtttt ccgtcgacca
      241 taacgcgaac tttttgtaag ttaggtttaa cagtacgttt tgttgagttc atagcgtgtg
      301 aacggttgtt tccacttttt gtcttgcgac ctgtaaagta acaaactttt gccatggtaa
      361 tttcctcctt tgccttgatt tggagcaata gaataattca gctcatacca gactaacata
      421 tcatatagaa tggaaatttg caagtacatt ttcatagata caattgacaa ttgagaagaa
      481 taattgtaaa ttaaacaagg ttatacctga aattttgcaa ggtttttgag tactttgggt
      541 aactgccttt tatttcaagt tgcgctatga taaaatatga tagaagaaaa atgggagttt
      601 tttacccgtt aaggaggctc attcatggct gtaaaaattc aaacgtcagc tggaaccatt
      661 gaaattacca atgatgtaat tgcaacagtt gttggtggcg ccgcgacaga tgtgttcggt
      721 gttgtaggaa tggccagcaa aaatcaaatt aaagataata ttactgagat tttgcgtaaa
      781 gaaaattact caagaggagt agttgtccgc caagaagata atggtattgc tgtagatgtt
      841 tatacgatta tcagctatgg tacgaaaatc tctgaagttt cccgcaacgt ccaagaaaaa
      901 gtgaaataca atctagaggc attattaggc gtaactgcca attcagttaa tgtctttgta
      961 caaggtgttc gtgtactgcc agactaggca ggagactggc tggtaactca gcagtcgatc
     1021 tgaaggagga ttatctaggt gagtgtaaca gaaataagtg caagccagtt ccaggaaatg
     1081 gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat acgtcaattc attaaatgta
     1141 ttccctgtgc cagatggcga tacgggaaca aatatgaatc tatctatgac tagtggagct
     1201 aaggcagtag tagattcaac ctcagaaaaa gtaggagaat tagcagcaat gctttcaaaa
     1261 ggattgctga tgggtgcgcg cggaaattcc ggcgttattt tgtctcaatt gtttcgtgga
     1321 ttttctaagc aaattccaga tgttgtctca ttaaatgctt ccgatcttgc tgcagccttc
     1381 actcatggag ttgaaactgc gtataaagca gtaatgaaac cagtagaagg aacgattttg
     1441 acagtagcta gagaagcagc gaaagctgga gaaaagaaag caaaaagtac tgatgatgtc
     1501 gttgaagtaa tgacagctgt tgtaaaaggg gcaaagcgtg ctttagctaa aactccagac
     1561 ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg gagggcaagg actgctgttt
     1621 gtttatgagg gctttttaag tgcattgaat ggagaattcc aagcggatga aacatatgaa
     1681 ccttctccag cagaaatgga tgatatggtc aatgcagaac atcatcgcag tgtccaaggt
     1741 caaatggcga cagaagatat ccattttggt tactgtacag aaatcatggt aaaaatcgga
     1801 gaaggaccta cagtcgatag tacattcgat tacgaagaat tccgtaacta tttagatggt
     1861 ataggagatt ctttacttgt ggtcaatgat gacgagatca tcaaagtcca cgtgcataca
     1921 gaaaacccag gagaagtctt gaattatgga caaaaattcg gttcattagt caaaatcaaa
     1981 gtcgacaaca tgcgcttgca gcatgagacg attttggaac acgatgaaga agtgaacgag
     2041 tttgcgaaaa aagaaacacc acgtatcccg tatggtatcg tagcgatcgc tgctggtaaa
     2101 ggcgtccaag agctgtttga aagtttaggc gctaattatg ttatcagtgg tgggcaaaca
     2161 atgaatccaa gtacggaaga cattgttaaa gcgattcaag aagtaaacgc agataaagtg
     2221 attgtcttgc caaacaataa gaacatcttc atggcagctg atcaagcagc agaagtagca
     2281 gatatcccag tagcagttgt tccttcaaga acgatttccc aaggattgac agctatgctt
     2341 tcattcaatg atcaagcttc tttagaagaa aacaaagaaa cgatgacaga aatgttgtct
     2401 agcgttgtga gcggtcaagt cactcacgcg atcagagata cctcaattga cggtgtagag
     2461 atcaaagaag gagacttttt gggcatgatc gatggaaaaa tcctgatctc taacccagat
     2521 atcacagcaa cagcgttaag tacactagaa caaatgatca atgaggacac agagatcgta
     2581 acgatcttgg caggtgatga aggaacagtt gaacaagcaa atgaatttgc tgagaaacta
     2641 acagaaaaac atcctgattt ggaaatcgag atccaccaag gagatcagcc tgtctatcct
     2701 tatctatttt ctgctgaata atttctgtgt cagaatatct ttctaacaca gtattcattc
     2761 ataaaaggcc ttcggttagt gatcaggtat gatcgctgac caagtccttt ttttgttaag
     2821 ggagggagga aaaatttatg cgttctttag atgatccaat cactatgctg gcaggagtcg
     2881 gagaaaaaag agcagctagt cttgccagct tgggcatcca aacgattgaa gacttactga
     2941 cttattatcc tttccgctac gaagatattc aggaaagaaa tctaaacgag atccaagatc
     3001 aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc tgtgatgaat cgatttggtt
     3061 ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca tgatgtgttc aacgtttctt
     3121 tttttaatca accttatcta aaagaaaaag tcattttatc ggaggaaatc gccgtatacg
     3181 gtaaatggga tgcaaagcgt aaagcattga ccgggatgaa gattttaggt tctcaaacaa
     3241 tggatgactt ttcgccgata tatcacgtca acaaatcaat tcgtcaaacg acattagttg
     3301 atctaatcaa aaaagggttt caagaatacg gcacattgat tgaagaaaat ctgccaaaca
     3361 accttgtgga gaaatatcga ctgcttgacc gagcttctgc tgtaagaagt atgcattttc
     3421 caaaagacca cgaagaaaac catcaagcca aacgacgtgt cgtttttgaa gagttctttt
     3481 tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa agcagaaacg aatggtttgg
     3541 agatccttta tgatgtccaa aaattgaaag aatttactca aaagctacct tttgaactga
     3601 caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga tcttcgcagt ccgcagcata
     3661 tgcagcgact tttgcaggga gatgtaggta gcggaaaaac agtggttgcc gcgattgcgt
     3721 tatacgctac cgttacagca ggtttccaag gtgcgttgat ggtgcctaca gaaatcttgg
     3781 cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc caatgaagta accatcgcat
     3841 tgcttacggg gtcaacgaaa gcaaaagaac gtcgtgagct ccttgaaaaa ctagagcaag
     3901 gggaaattaa catcgtgatc ggcacacatg cattgatcca agatggagtc gaatttcaga
     3961 atttaggtct agttattacg gatgagcagc accgttttgg cgtgaaccag cggaaagtac
     4021 ttcgtgaaaa aggctggcga ccagatgtct tatttatgac agcgactccg attcctagaa
     4081 cattagctat tacagcatat ggtgagatgg atgtatctat cattgatgaa cttccagctg
     4141 gacgtatccc gatcgaaaca cgctgggtaa gaacacctca attggacagt gtactggatt
     4201 ggacgaaaaa agaactggca agaggacatc aaatgtatgt catctgtccg ctgattgaag
     4261 aatcagaagc attagatgta aaaaatgcag tggaaatcta tgaaaaactt cgtgaactat
     4321 ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat gaagaatcag gaaaaagaag
     4381 taatcatgga agccttcaaa gacaaccaga tgcagatatt agtttcgact actgtaattg
     4441 aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat catggatgcg gatcgatttg
     4501 gattggcaca gctgcatcaa cttcgtggac gtgttggacg cggaagtgat gcttcctact
     4561 gtatcttagt tgctaatcct aaaaatgaac taggtgtgga acggatgaaa atcatgacag
     4621 agaccaccaa tggttttgta ctaagcgaaa aagatctgga attacgtggt cctggtgaag
     4681 tatttggctt ccgtcaatct ggtctgcctc aatttgcgat tgctgacttg gtgacggatg
     4741 gaaatgtttt ggaggtagcc agagaagagg ctgcagccct ttggcagata aaggattggc
     4801 agatgcttcc tgagtatcag cctttagcgc aaagcctcca aatagaaaat caggataatc
     4861 gttatttcga ttagcagact gtgctatact ttatactgat gacggaaaaa ggagtggaaa
     4921 caatgaaaat tgccgtagat gcaatgggcg gagatcacgc acctcaagca attgtagaag
     4981 gcgtggcttt agcgcagcaa gattttccag aagtagaatt cttactttac ggaaaagaag
     5041 cagaaatcaa gaaatactta acaaatgaaa aaaacatcac gattatccat acagacgaaa
     5101 aaatcaatag tgatgatgag ccagtaaaag caattcgacg aaaaaagact gcatcaatgg
     5161 tccttgctgc acaagcagtg aaaaacggtg aagcagatgc tgtattttca gcagggaata
     5221 caggcgcgct actagcagca gggttgttca tcgtaggacg tatcaaaaat atcgaacgcc
     5281 ctggcttgat gtctaccttg ccagtagtag ggaaagaagg tgccggtttc gatatgctgg
     5341 atcttggcgc taacgcagaa aacaaaccag aacatttgtt gcaatacggt attttaggtt
     5401 ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg tgtcgcactc ttaaacaacg
     5461 gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc ctatgaactc cttcagaatg
     5521 aaacaagctt gaattttatt ggaaacgtag aagctcgtga gctactcaat ggtgtggctg
     5581 atgtagtggt cacagatgga tttacaggca acgctgtttt gaaatctatt gaaggaacag
     5641 caatgaatat gatgaatcta ttaaaatctg ctattctaaa tgaagggatc aaaggaaaaa
     5701 tgggcgctat gcttttaaaa gacggcttac gttctttgaa agcagaaatg gattattcca
     5761 aacatggcgg tgccgttttg tttggcttga aagcaccagt cattaaaact catggagcaa
     5821 caggccccga tgctgttcgc tatacgatcc gccaaatcca cacaatgctt gagacagatg
     5881 tagttggaca attagtcgaa caatttgaaa aaagcgaaga atagtatttg gatatctgaa
     5941 gcaatccgtt ccattgaaaa aaaggtggac attcctgaaa aatgccagta aaattagcca
     6001 tggtgtaggt aattgacaag tgtatgattc ttggaggtga aaaagttgac tcgcgaagaa
     6061 gtatttaaca aagtagcgaa aattatttca aatcattttg agatagatac cgataaagtg
     6121 acggatgaat tgaacatcaa agatgattta aaagctgatt caattagtat tatggaattc
     6181 gttttagagc ttgaagatga attcggtacg gaaatttcag atgaagacgc tgaacaaatt
     6241 gaaacagtcg gtggcgcagt agattatatc agcagccatc ttaaataatt tcaaataaaa
     6301 gcaaagaagg ataaaaagct gcatacagct ttttatcctt ctttttttaa tctttttgac
     6361 acgtaaaagt catttttctg ataattctgg cgataaacag aaatta
//
LOCUS       DAXWLB010000150         6406 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXWLB010000150 DAXWLB010000000
VERSION     DAXWLB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.153"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..274
                     /locus_tag="ACGE4H_002348"
     CDS             <1..274
                     /locus_tag="ACGE4H_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365732.1"
                     /translation="VVKDIALDLNYTLAKMNSVIQQAESFCERYPEYKLSFLSENKMI
                     KVEFSSQFLLSKVYSILLEGTIGYILLDSLYKGTYQSLENLSQKII"
     gene            319..1434
                     /locus_tag="ACGE4H_002349"
     CDS             319..1434
                     /locus_tag="ACGE4H_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365733.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1460..1900)
                     /locus_tag="ACGE4H_002350"
     CDS             complement(1460..1900)
                     /locus_tag="ACGE4H_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8365734.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2060..2566
                     /locus_tag="ACGE4H_002351"
     CDS             2060..2566
                     /locus_tag="ACGE4H_002351"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFF8365735.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2734..4647
                     /locus_tag="ACGE4H_002352"
     CDS             2734..4647
                     /locus_tag="ACGE4H_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8365736.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4652..5653
                     /locus_tag="ACGE4H_002353"
     CDS             4652..5653
                     /locus_tag="ACGE4H_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFF8365737.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5855..6061)
                     /locus_tag="ACGE4H_002354"
     CDS             complement(5855..6061)
                     /locus_tag="ACGE4H_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFF8365738.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2363 a          978 c         1262 g         1803 t
ORIGIN      
        1 tgtggtgaag gatatcgctt tagatctaaa ctacaccctg gctaagatga attcggtgat
       61 tcaacaagca gaatcgtttt gcgaaaggta tccagaatat aaattatctt ttctttcaga
      121 aaacaagatg ataaaagtcg agttttcttc tcagttttta ctgagtaaag tttattctat
      181 acttttagag ggaacgattg gttatatttt actggattca ctttacaaag gtacttatca
      241 atccttagaa aatcttagtc aaaaaattat ataagtttgc gtacgattca aagaaaatta
      301 agagaactta atactatttt gaaaaactat catttatctt gcagcttgaa aaaaggtaat
      361 ccgatagaag gagaagaata ccgtatccga tacttttttc acttgatgta ttggcagtca
      421 ttcgacgaaa atcattctga tatattatca agtataaaaa ataaacgtca actattagaa
      481 aaatttgcga aacatgcgcc atatacgcgt agtatagata gaaagaaatg gatcagccta
      541 ctaagaattt cgttgcaacg aatgaaacaa ggccactcga ttacgtatgt cccagaagag
      601 atgaacaaat ttgtgcatcc attgataaca aaagaagagt ttacgcatta catactagag
      661 cctttttttg aagcaaactt ctggtcgatg aatcacatca ctccaacaga gcaagcttac
      721 ctttatttta tgtttggtgt aatgaacacc tatttggaag aagatttgtt ggatactcca
      781 gataaagtac aagaaagttg gggctggatt gaatcgtcta ttgtagaaaa ttttgaaaaa
      841 aacttcaaga tttgttttac aacttcagaa aaaaattatc tatatgtcaa tttggcaatg
      901 atccatttgt attcaagggt ttttgggaca aaaaagaagg tggatgcttt tggaaaatca
      961 gcagaagaca gagattttca gcatatattt ccagtgatgt atccaatcat gcaacagttt
     1021 tttagtgggg tagctcgcag aattccagag cttaatactt attatgaaaa gaacagacgg
     1081 ttgatttttc aatactgtat gttagtcagg gatattttta taaaacacga acaacctgtc
     1141 atattctata ttcaatcaaa gtatggtaaa acacaggaat tatgggcaaa gaaaaggata
     1201 ttatctatga ctgaacgccc aataaaatat agtgcatccg ctgatcagtt acctgatatt
     1261 gtcattagcg attatcctat cgacaaaaag gcatatgatc aaacgaatac acagatattt
     1321 tactggaatg gacaacctac aaaaaatgat tgggcgcgtc tgaaagctga aatcataaaa
     1381 atcaggaatg agaaaaatag atccatattg gaaaaattgg atttcgttaa ttaagaaaag
     1441 cagattgttt atgccaaaat taagacattg caaaaagatt attttcttta aaatcgacaa
     1501 gatgaccaga aagcaagagt tcgttgagct cttgctttct tttagtttta gaaaggaaca
     1561 tcctgatttt atcttcgcta aatccagtca aaagttttgc agattggtcg tagataatcg
     1621 gttttttcaa taaagaaggg gatgtacgaa tctcaatata tagttcactc aaactgagtt
     1681 catctattct gtgaacaaga tttttataac taatagaatg agtagagagc atttcggtca
     1741 cgccgttgtt tgtcaatttt agtaagtgga taaattcatt tttggttagt ggttgacgaa
     1801 ttaagttctg ttcgataaac ggtatatcat ggacgataag ccaacgtttt gcgcgtcttg
     1861 aagacgagca gccagaacct gtatacaatt taatcatcat acattcctcc aatagctaag
     1921 tatttatgaa ataataacat gaagaaatag tcgaaaagtg ccatttagag gtgtaaaata
     1981 agaaaatgtt gtcacgtata atggaactag ctagataaga aatactatta aatcaagaaa
     2041 aaagaaagaa ggcacaagta tgtcaaacac agccgacgca atagtagtga tcgatttaca
     2101 aaaaggggta tgttttgatt cttcgccgat acacgaatta gaaacagtga cagcattagt
     2161 gaataaaaga atcaaaacat acgaaacgat tgggaaacca gttattttcg ttcaacacga
     2221 agatgctcaa ttagtgccac aaacaaaacc atgggagcta ttgtcggaat tatctattcc
     2281 tgagcacagt tattttgtga gaaaaaccca tgcgaattca ttttataaga ctaatttgag
     2341 tgttttatta actgaattgg actgtcattc gatcgaatta tgtggggcac aagcagagta
     2401 ttgtatagat acgactgtga agtttgctca tggattagga taccagctta tgatgtgtag
     2461 aggagcaacg acaacttatg ataatccttt tatgaaagca aaggaaacaa tcgcttttta
     2521 cgagaaaatt tgggataaac gatttctgac atttttggag caataagttg tttcttcgtt
     2581 caaaaaaagc agaatggata ctggtttatc cattctgctt tattacgtaa aatgagaaaa
     2641 taatcctgtt ctatttttat gggataaacc atttattttt tgtataaaaa atgtttgcgt
     2701 ttacatataa ttctcctaga aacataaaaa ggagtgaata gcttgagaac aaaacaagta
     2761 ttaaagggag cgttggtcat cttatttgct gtaagtacaa gcgtcccgca atggacagta
     2821 tatggagaga gcatttcttc cgaatcggaa tctcgggagg tagaaaccac agaaactact
     2881 gatgctggaa atgaaacgaa atcgagtact tcacaaacag aaacaaatac tgaaacaacg
     2941 gttgagacat ctgttactag cacagatcaa gaaacgaccg ctaccacaga taaccaacaa
     3001 acagaagaag caacagctac aaaaatgtca aacgacgaat cctcttctgc taaagtaagt
     3061 gacaaagaga atgggacaga aaatgatgat atcggtaaag gtgaaaattt caatggtaaa
     3121 gaatatttga attacttcgg taaacagatg acagaaatac ttaatggacc aacagaagac
     3181 atcaagtcaa aattaataga gttattagtc actgagcttg ctgttaatga ttatggtgta
     3241 gataaagaga ttatcaaaca tctgattgct acttatctat tgtacgatat cgcttttgca
     3301 aaaaacagtg tgttgatgtc gatgcttgga ggtacagatg atgggacaac gcctacagaa
     3361 aacacaggct tctatccaaa tacaaaacaa cttcatcaaa ctgtactaaa caaacaagat
     3421 ggcaaaatga tggatgtata tgcatattat gtcgatcaag gttcagataa aacagtcatg
     3481 atccatggcg gatttcgagg gaattggaat aacgggatcg ttacagaaga atacaatgat
     3541 ttttataagg ccgggtataa tttattattt gttgattcca gagcgactgg taatagcgga
     3601 ggcgattatg tgacctatgg acaatatgag tcagatgacg tcctttactg gataaatcaa
     3661 gaagttcgag agcggccatc tcaaaagatt ttactttatg gtggttcgat gggagcagct
     3721 acgatgatgt ctgtattagc gaaagatatt cctgtcaatg tcaaaggaat tattgaaaat
     3781 tgcggatttg catcaattga tgaacaacta cgttttacgt acagccagac agtagccccc
     3841 gcactaccgg atgccataaa aaatcagtta gacatcattg gggatcaaga gcatgaagat
     3901 ttatttatgg gccttttgaa acaatactat tttgatcagg agatgcattt ggatacaaaa
     3961 gctgctctac ctacgattgg aatgtcagat tcattgccta aattgattat ccatggaaca
     4021 gcagatgatg tagtacctgt gtctaacgcg caaaaattat atgagttatc aggagggtat
     4081 aaagatcttt tattagtaga aggagcaggg catggaaagg cacaggaagt tgaccatgct
     4141 gcctacacga aacatgtaac ggactttttg aaagtagtgt tccatgatca gataaacgta
     4201 aagtatgtgg atgaaaacaa taaaagtcta ctcaaagacc aggacgaaat ccagctttac
     4261 ggagcttatg gagaaaatta tatgacagag caaaagacat tcgaaggata cgaattagca
     4321 aatgttgaag gaccgactga agggattttt aatgagacga taccaactat tattttctat
     4381 tacaaaaaaa tcccagtagc tccaccaaaa aagcaagatc cgacagagaa tgcagacaat
     4441 aagggaaaga atgaacctcg agaggatcaa tcaagtgatg atacgcaacc gattcagatg
     4501 acagtgaaag tacaaaaaaa gacagatgca aaaaaaagaa tgttgccgaa aactggagaa
     4561 aagaaacatt cgcttctcat ttattcaggt ggatttttat ctgttagcac gattatcaca
     4621 tggatttggc gaagaaagag agggtaaatg aatgaaatgg ttcaagcgaa tagtgattgc
     4681 agtaccagtt atcatcgtag ttattttagt cactgtattt ttgattaatc aaatcacacc
     4741 aaagccgatt agcttgcttg ttagaaaaca atttgacaca agtgaaaaag aacaagtata
     4801 tgcccgacca gatgatttcc agcaaaaaat cgaacaaatc gatataaaaa aagaaatatc
     4861 ctatccttca aagtacaaaa ataataatat ggatatctac acaccgaaag tggaaaagaa
     4921 aaaattaccg attcttttct ggatgcatgg aggcgcatat gtaggagggg ataaaaatga
     4981 ttgtcgtgat tatttggaat atctatgttc agacacgcag caaatcatcg taaatataga
     5041 ttatgaacga tcaccggaag caaaacatcc tacaccagtt atccaattga acgaagccat
     5101 tcaagcagct aaaaaagaga taaaagatca agcagattgg tcgaatattt cgattggtgg
     5161 aaattcggca ggtgcacaaa ttagtggaga atatttgtta gcacttcaaa atgaagaaat
     5221 taaaaaagct gatcatcttg atccgacttt ggagaacaag cagattacga agtttatttc
     5281 tttgtctggt ctgttggatc cttctgcact tactcaagta agtgataaaa tctctagctt
     5341 tctttatgaa aaatgtggat gggcttactt tgataataag gactttgaac aatcagagaa
     5401 ggtcaaaagt ttagccttgg tcagacatgc ggatcgttgg acgcaaaaca cattcttaac
     5461 ggatggaaat acaaatacgt ttacgaaaca aatggaagaa gtaacttctg tatttgagaa
     5521 agcgggggtg aaagtcacaa aagtagatta tgcggagaaa aatgctgttt tgaaccatga
     5581 atatcagttt gatttcagca ataaacaagc gcaagaaaca tatcaaaagt tagtaatgtt
     5641 tttcaaggag taaagtctta tgatgcaaaa gaggttgtaa tctgtcactt agctccaagt
     5701 cacaaggagc aatttcaata aacaagcttt tcatggttta tgaatattgt aacttgtgac
     5761 tgaggagtta gagatggcaa ggggcacctt ttattcgaaa tttgggaata tcacacgatt
     5821 attgtcacga tctcttttac gtaaatttta taagtcagat ttgttgtttt gatcgtttaa
     5881 aaaaagcaac aaatccgaag cctgcggagg caagaaggga caaaaagccg agtggtaagc
     5941 aaaagaatgc tggttccact aatattccgt tgttattgat ataaggctgg gaacaaagat
     6001 gaatagcaaa taaaatcaat gcactagcga aaaaaagcaa agaaatatgg atagctttca
     6061 taagcagcac ctcctttttt ataattgtat gataaataga ttaagaatct ataaatgtga
     6121 caagtttgtt aggatgtgaa taaaatgatc atctagcgaa cttgaaacaa agatggctaa
     6181 tgataagcaa ttaatgattc agcaatcaga taggtacaaa gtttaccatc atacgtctag
     6241 accaataata gaaaatatgg gaatataata gaaaagattt aggatacccc caaaagaatt
     6301 tccccaaaaa ttcgatgtat cctaaacttt tttattttac ataacaatca tatcaactgt
     6361 gaaataaaat tacaaatgat aacctaaaat gaggccaaaa gccagt
//
LOCUS       DAXWLB010000151         6399 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXWLB010000151 DAXWLB010000000
VERSION     DAXWLB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.154"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4H_002355"
     CDS             complement(31..633)
                     /gene="nrdG"
                     /locus_tag="ACGE4H_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFF8365739.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4H_002356"
     CDS             complement(948..3137)
                     /gene="nrdD"
                     /locus_tag="ACGE4H_002356"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFF8365740.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            3463..4230
                     /locus_tag="ACGE4H_002357"
     CDS             3463..4230
                     /locus_tag="ACGE4H_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365741.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            4220..6262
                     /locus_tag="ACGE4H_002358"
     CDS             4220..6262
                     /locus_tag="ACGE4H_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8365742.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         1901 a         1131 c         1226 g         2141 t
ORIGIN      
        1 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
       61 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
      121 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
      181 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
      241 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
      301 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
      361 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
      421 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
      481 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
      541 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
      601 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
      661 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
      721 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
      781 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
      841 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
      901 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
      961 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
     1021 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
     1081 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
     1141 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
     1201 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
     1261 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
     1321 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
     1381 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
     1441 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
     1501 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
     1561 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
     1621 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
     1681 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
     1741 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
     1801 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
     1861 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
     1921 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
     1981 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
     2041 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
     2101 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
     2161 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
     2221 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
     2281 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
     2341 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
     2401 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
     2461 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
     2521 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
     2581 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
     2641 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
     2701 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
     2761 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
     2821 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
     2881 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
     2941 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
     3001 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
     3061 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
     3121 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
     3181 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
     3241 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
     3301 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
     3361 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
     3421 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
     3481 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
     3541 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
     3601 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
     3661 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
     3721 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
     3781 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
     3841 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
     3901 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
     3961 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
     4021 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
     4081 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
     4141 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
     4201 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
     4261 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
     4321 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
     4381 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
     4441 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
     4501 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
     4561 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
     4621 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
     4681 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
     4741 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
     4801 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
     4861 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
     4921 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
     4981 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
     5041 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
     5101 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
     5161 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
     5221 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
     5281 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
     5341 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
     5401 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
     5461 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
     5521 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
     5581 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
     5641 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
     5701 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
     5761 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
     5821 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
     5881 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
     5941 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
     6001 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
     6061 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
     6121 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
     6181 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
     6241 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
     6301 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
     6361 tttttatatt ttttaaaagt acgagaaatt ataaagaaa
//
LOCUS       DAXWLB010000152         6380 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXWLB010000152 DAXWLB010000000
VERSION     DAXWLB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.155"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            107..445
                     /locus_tag="ACGE4H_002359"
     CDS             107..445
                     /locus_tag="ACGE4H_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365743.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            531..776
                     /locus_tag="ACGE4H_002360"
     CDS             531..776
                     /locus_tag="ACGE4H_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365744.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            796..2952
                     /locus_tag="ACGE4H_002361"
     CDS             796..2952
                     /locus_tag="ACGE4H_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365745.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            2964..3197
                     /locus_tag="ACGE4H_002362"
     CDS             2964..3197
                     /locus_tag="ACGE4H_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8365746.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3187..4053
                     /locus_tag="ACGE4H_002363"
     CDS             3187..4053
                     /locus_tag="ACGE4H_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365747.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4068..6038
                     /locus_tag="ACGE4H_002364"
     CDS             4068..6038
                     /locus_tag="ACGE4H_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365748.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
BASE COUNT         2453 a          876 c         1247 g         1804 t
ORIGIN      
        1 aaatatcgag acgtcttttt cttagttaga tcattgacaa gtgaacaaat aagatttgtc
       61 gatgatataa ttaacttatt aaaaatatta aaaaggaggt ttttatatgt tcgattctat
      121 taaaaatttg gttagcggaa tgacgcctga tatgaaaact ttagtacaag ttgttttgta
      181 tttcttggca ttcgtttttt cgttgaaacc aggattcact gcaatgaaat tttttggtga
      241 tgcaaaatgg ggagatggat taaaatatgt tggggcgatt attgcaatct ttattattcc
      301 tgtaattaca accgttatgc tttacacaat gggtactaaa acaggtaatg atttgaacca
      361 aaaagctaat atcatatcag cacttattcc agttgttggt atctatctag tttcaaaatt
      421 tccaaaaaat gaactgttag gttaaaagtg aattgaacaa atttggataa tatgccgatt
      481 tgttcaattt actttttacc aaaaaactac gctccaagcg tagtttagaa gtgagggaag
      541 aaagtaaatg taagatggtt tttgatagag aaaaaatgtt agctcacgcc aacgaagtat
      601 taatgagcag tctgaaaggt accgaactgg caaagataat gaatatgaat gttaaccagt
      661 tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc cagacttgaa acgcttatta
      721 aattcgaaaa agcatatgta tatatgctag ataaacagaa aagaactatt gactgaaaaa
      781 aggagtttta caataatggc aaaattcaaa gatattaccg aatctattaa ttatgtggca
      841 gaaacagttc ctgtagttgc actctctttg aagccacaat tactcgaagt gtacaaaaaa
      901 gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg tatggaaaga attcgatgcg
      961 ttgctgacca aagatttacc aaagaaaaat tatatggtaa ttgaagatgt acaaaagctc
     1021 aacagtgagg gagttataga tgaggatgat gctattctcg attctgagaa gtatgtcgtt
     1081 ctattgaagt caagtttgaa ttatgtagct gatctaccag aaaaatttaa agagaagcta
     1141 ttagcaggtg tccgttttat ggatagtagt ggtcaattat tagttattcc ggatgatcct
     1201 aatgtgtttg aatcagaagt tgaagaagct acagacttct atagcgcagt agagcaatta
     1261 gaagtagcta ctaatggatt agaacgaatt gctgagacct tcaactcttc tgaagaagtt
     1321 gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag aagctctttt tgtggatgat
     1381 aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag aagaaacaca aatacaaaca
     1441 gaaacctacc aacaaccaaa aattgatgaa aaacccgaat cccaaagtga caaagaaaat
     1501 tatgtttatg aagctgaaaa aatcacatct gaaagtgatg taccgaaaaa cttagatgct
     1561 gattttttag aaaaaaattt tgaacagatc attgctgaca aatttcaacc tgtagtttta
     1621 gatgagatta atgaccctgt cgatttaaac gaggagatgc tatccatacc tgagttgaaa
     1681 ccattgctgt ttttagctaa agagagtatg aacgctcgca tctctattgc taataataaa
     1741 atcaacagtg aattaaataa gacgaaggag caattgatcc tcaaaggaaa gcaaatgtta
     1801 gctgatgagc ttttgcggat agaggaagca acctcattaa atggtcaaaa tcagtttaca
     1861 aatacactat cggttgctaa agagagctac gaagcagaag catctaaaat tgatgattta
     1921 gtgatggaaa aatccaatat cattcgtaaa gattatgata tggcgaaaca ggcttatttg
     1981 gatgctgaac taaagaaact agagcaacgg tatgatgctg agcatttacc agaagttaaa
     2041 caacaagaag atgaatatag aaaagcaact gaagaaagtc ttgatcaaca gtaccaagaa
     2101 gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc aagaagcaaa agaagttgtg
     2161 gtggaacgaa ttatcaattc attgaaacca gaaattgaaa aatcattaac agacgtgcaa
     2221 gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa atgaagctga gttccatgat
     2281 acaaaagaga aaacaacaga tgtggttgtc aaatattttg cgaacaaaga agaagttgaa
     2341 aagcaggtag ctaacaacac gaaagaatta gaaacaaaac ttgtagagca acaaaaaact
     2401 atcgaagaat ataaagatcg aatgaaagag gtcaaagatt tagcagaggg ttataaatta
     2461 aatagcgaaa agttggcaga acaaagtgaa gctttaagag aagacttatc tcgttcccga
     2521 agtgatctag ctcaaatggc agaaaaaacg aaatcattag cagttgctac agaaaatcaa
     2581 tcaagttcaa aacgtgattt tacaagtaaa aagatggcta ctatcatttc tgtcgctgca
     2641 atggcttgcg gaacgatctt gggatcgtcc tactttatta gtcataataa tcaacaagca
     2701 caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt cgattgggac aaatacgaac
     2761 tctgcttcaa caaatagtag ctatgaagca acaccaacta cagagttcaa agttgggaaa
     2821 gatgttgtca tcagtattga tgggaaaaca actaaggctg aagtgatttc tattgatgga
     2881 gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca aaattcaagc tcacttatct
     2941 gataataact aaggagggct attatgcgag aagaaaaaat agtttatcca gtaattcaaa
     3001 acaatcaaga aaacatgacc tcttctcagc acttacagga agcacagctc gtgtatcagg
     3061 taaatgaact accacaaacg aacgagacaa tgagcaagtc aatatcttta attggcttgc
     3121 ttattgttgc aatagtgata actgtaggtg tttaccgtaa gttgaacgtg aaaggaagta
     3181 tttctgatgg cagataactc aaactatgga tttaaacata acttacatca aaaaccttct
     3241 gaaacaattg tcagagttga atggcttgaa aagcatgtca atagcaccta ttctgttaca
     3301 gcaaaaagat taattgtatc agctatagtt gttgcagtcg ttgttcttgg atcaattgtt
     3361 tacttagttt ctgatacgat taaactgaaa tttatttttg ttccaacgtt attaatgata
     3421 attggatttc ttattctaat tagccagtat atgtttattg atccatctag tggttcattg
     3481 atttctggtt ctattagact gttcaaatca gtagttaatt cagcaagagt atctatgggg
     3541 aaaaagaaac catttcgagg cacagagatt ttaaaagtga aacaagatgg aacaatcgta
     3601 tatcgagatg ggagtgtagc attaatgttc aatctagatg gggctacatc actaaccgct
     3661 tttccagaag aagttatggc tcaggaatcg aaatcaatac aatatcaaaa taccagagat
     3721 cgttccttag ctgaaatcaa aatcacttca tctcaacctc agaatacgga aagacaaact
     3781 cgaaatatga aaatacaaaa acgaaatgca aggagtaaca aagcagtctt tgacataata
     3841 gatcagcaag aacgatttgt atcagaatat attgatggtg tgaaaacaac agtaatccaa
     3901 tatcttatct tgaaatctca atctaagatg gtattggatg aaggtgtgga acgtttagaa
     3961 aactatacga gtagaggact ttattatagt gtcactcagt tgtcagctaa acaaacaaaa
     4021 gaaatattat ctgatattta tgcactgaaa tagaggtgag agattaaatg gaaattatgg
     4081 gtagatcaca actattaaat ttcaaaaaaa agagaaaaac taaaaaagaa caaaaaacta
     4141 gaaattcaaa ggtagtgaat aatacctttg ttagtgattt aacatcttta gttggttatc
     4201 aatttacacc atcttacatt aggacaggta agcggtgggg aacaattgtg aaattagtga
     4261 atacttatgg aatgaataga aattatggat ttggttggat ggttaaagct attcctgaag
     4321 tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga taagccagtg tcacaaaaag
     4381 atcaacaaga aataatgcac gagaatgttg ggggatcaac gaatagttat gaaaataatt
     4441 tagaaggaaa aaattcagat gctgaaatta gcaaaactgt tatgcggatt atggatttga
     4501 atacagcatc tttactagac agcatggcaa ataaaattat tgattggaag ctagaaatcc
     4561 tattagtatc cgattctcct aataaaatca gcgaacagct aattaaactc caacggttgt
     4621 atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg aggagatcaa cgtgatctgt
     4681 ttgatagact tttaagtgaa ccaatcggga atgtctatga gtatacgtct atgagttcgg
     4741 attttgcagg aaatgatcat gcaattcgca aaggtttaga tgatgcgaat ggatgggcta
     4801 tcggcactat gtctactagc tacgcaagtg gtgtagcttt tatggcactg gatgattctt
     4861 ttacaagtaa agttttagtg gcatcacaca aaaacgctac aattaaaggc tatgatgaaa
     4921 aaatctcagc atcatcactt tgggggcaat tgattgctaa taatgcaatg gcaaatcata
     4981 aacgaacttt tcatattgtc ttaaataaat ttgattactt tggtgatcca actggtagaa
     5041 catttgttgc caaaccttcg ttaaacagtg taatagaaaa ggttaatttg gctgaagaag
     5101 gtcttaactc tttggagctt tttggtaatc aaaaggacat tggtgattta tatagacgga
     5161 atctcgataa actaactcag attttttatt tgttagcagg cagaacatta gattctgcaa
     5221 ctagagtttt attgaaaaaa gaattaggta acttttatat aagtcagaaa ctttgggata
     5281 ttaattatga agaacgttca aaacttttgg tgaacagtag tgatacaatg ccagttcttg
     5341 gggattttat tagctatttg actaatgctg aaacaaaagc caaaaaatct ggtactgaaa
     5401 aagaagtcga tcgtattatt gtattaaaag acacattgag aacagcatta gacagcttct
     5461 ctaggatgtt taatcaaaga acgacactga aagaaatcgg taaaagtgac aaattacagt
     5521 tttactataa cttaggtgat ctaagagcag aaaccaatgt tatggaagct caatttttaa
     5581 atacctttga tttcatcaca tcacaagcaa aaaaagatga tattgttatg attcacggct
     5641 tcgataagtt atctattgaa accgttgggc taattaaaaa aagattaaca atgcttgaag
     5701 aagatggaat tcgtcttgcc tttctatttg atacaatcgg ttcaggagaa gacaatgaat
     5761 acaaagaaga tattgaaaca gctgatatat tcaatacaga tggaaaaatt taccaaagtt
     5821 ttgaaagtga ttttaattac actatcattg gaaagatgac cagtggagag ttaatgaagt
     5881 atgaaaaaaa agttgctcct gctagaaaag gagaagatga tcaaaaactt tctgttgatt
     5941 tgagacagta tttgacaaca ggaagaagta ataaatttca gatcaggaga ccagtggatc
     6001 tgactaataa ttttgttatt ggagagttta ttctttaagt tttatctttg attttgagtg
     6061 tataaggaaa ggataagaaa aaacatgtat atttgagact aaaaacagtc ttagagaaca
     6121 tggtacgttt tgtgaaaaga gaactataga aatttcataa agtgaaacct tactgagtga
     6181 aataattata gtggaggtta aataatgaaa ccaaacataa aaattttaga tcgaatattt
     6241 ctaggtagag acacagaagt aatcctaatt caacatgaag aaggatttga agttagtata
     6301 ggtattcaga aacttcaaaa acctcattat tgtaatcagt tgtataaaaa ctttactgat
     6361 gaagaaaaag cgagagtttt
//
LOCUS       DAXWLB010000153         6356 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXWLB010000153 DAXWLB010000000
VERSION     DAXWLB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.156"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(1014..1679)
                     /locus_tag="ACGE4H_002365"
     CDS             complement(1014..1679)
                     /locus_tag="ACGE4H_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365749.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1805..3841
                     /locus_tag="ACGE4H_002366"
     CDS             1805..3841
                     /locus_tag="ACGE4H_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFF8365750.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3845..4483
                     /locus_tag="ACGE4H_002367"
     CDS             3845..4483
                     /locus_tag="ACGE4H_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8365751.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4511..4939
                     /locus_tag="ACGE4H_002368"
     CDS             4511..4939
                     /locus_tag="ACGE4H_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365752.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5396..6307
                     /locus_tag="ACGE4H_002369"
     CDS             5396..6307
                     /locus_tag="ACGE4H_002369"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8365753.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2200 a          965 c         1176 g         2015 t
ORIGIN      
        1 taaaatgaag cgaaaataat ttcttttaaa gagccgagat tatcagctat ataagagtga
       61 atttgatgca aaaaacagag ccgaaacagt cttggtgttc gtagctctgc ttatatgttt
      121 cgaatttaag acaaagagtt ggcaaaagat gttgataagg aagataagat aactgtgtta
      181 tataaactag gtaactaact gtaacaagct aattttaaga gattcttatt agaagacgtt
      241 taggaatata actaagaaaa tgattataca ggtaattgtc atttcaataa tgttttctaa
      301 ttttcgcgtc tttgttttaa gaaaaaggcc agaatcagtt ggaaaaaagt tcgtacgata
      361 gtgaagaggg gcaaaatttc ctactaatat tcccactaac aagagataaa acggccaggt
      421 tggtgtgatt cctctataaa aaaatgccca tgctaccgaa atcccataga ttaacaactg
      481 aacaattttt acaatgaaaa aattgttctg ccattactca aagtctcttc ctccttgtat
      541 cgtttatttg tttcatttta tcattatgat aggcgcttga acaatccatt ttgtatttta
      601 ttgagtactc ttggataaac taccagcgat tatcatattt atttatgtta ctgtaagcca
      661 tcatataaaa gtaaatgagc agatcataga aatataaaaa agaactaaaa ttgatctaaa
      721 ttatgtgcag cttgactacg taccaatcta tattacttac tagattggtc gttaatctat
      781 tcggatcgaa tgagcaaaat atttttgtga atgtccatcc ttgattatga ttccaaaaag
      841 aatcattggg aggctgtggc gtaaatatta tactataagc cagaaatacc tatcattctg
      901 aactttttta tttaggaact ttcatctcaa ggctttttca attaatttgt acccctgttt
      961 tttggataag atcatgtgca ttgatttttt ctatctctac gttcatctcc tacttaatgc
     1021 ctatctggct cctgagggct tctttttaag gcgtcgtcat agtcttggct gaggatttct
     1081 tggtaatctg agggaatggc ttcccaagtg agcaggcgaa cgttcatgac gtgttttcct
     1141 ttgtgtttga ttacagcatg gtttttctct ttttctacct ctccagcgtg aaaagacagc
     1201 catgtaccat ggccaaagat atcattacat acccaacgtt cgatggaatt gtccacgtat
     1261 cctacaggga gtgtggtata gccgatttgt ggtttcaaaa aaggattgaa tcgatcgatc
     1321 gtttcatttt tgtattgata attgataaga aaaagataga ccaaacaaaa gacgagcaga
     1381 aagaaagata gtgtcttttt gagtgagaag ggttgttttg atgaataaga tttcttgtta
     1441 acaagaggtt catctggcaa tgttggttct attgattcgg tagctgattc aatagaagga
     1501 aaatcaatcg gcagacctaa agtatgatgt aaaagtgaca gcatttcaaa gtctggcatt
     1561 gtacgagaat tttcccaact agaaatcgtt ttatcggaaa caccaatttt ttcgcctaat
     1621 tctttttgcg taagcttaca atttctgcgg taagttcgta ttttttctcc ggtgttcatc
     1681 ttcttctcct ttcgattcta cagtatatag atttattcta catgacctat acacttgata
     1741 gatacgtttt tttgatgaaa aacggtatga ttttatcaaa gaatcattta agtaagggag
     1801 agtggtgata atcaaaaaaa agttcacagt tttactcaca atcatagtga ccggtatcat
     1861 gagtggtttt gccttggtta gtataaataa acaggtactc tatcggacaa atgaattttt
     1921 ccatcaaaca gatacttata ctccgattcg attgccacaa cattttattg ggcaagaaga
     1981 atatgagcag ctaatcatgg tatttgaaaa tgtctcatca gaaactgagc tttcttatat
     2041 gaaaaaaaca gtaaatgaat ccaatgaagt agatacatct tggaacttca agatacagcc
     2101 aaagcttgaa atgacattgt attatcaagg ggatgtcaaa tcttctcacg agaaggtacc
     2161 ctattctaat acctcctttc gcgtaacgtc cattgaagaa tcgatcaaaa taaaagattt
     2221 tgaaggtgaa tttttcttga tgacgagaga aaaagaagcc tatgaacggg tcatttcact
     2281 attcactgat cgattcaatc aagcatttca cactgattat gccactaata tttttgaaga
     2341 ctttgaaaat agctattcgt attcggttat ttcaaataca gacgtatata attatcgagt
     2401 gtatttaact attggattga ttcttttatt ccttttgtta tcatcctttt tcttattgat
     2461 gaataaagaa atcagtttat tgttgaataa tgacttttca ttacatcaag tattgtggtg
     2521 tttattgggg aaaaagaaaa tcattattac attcataatt gcaggaatca ttggtggttt
     2581 tatttttatg tattataagg agcaatttct ttatttgttc cagatgtacg ttttcttgtt
     2641 cagcggaata tatggtatct ctttgatttc tcttttatta gttgaaaaaa tcgcttcgtt
     2701 aagagaagcg ttcaactata tccaatacat tctttttaca tttggattgt tgttttctct
     2761 tcttttgacc gttgccaatg tcgaattagc aactatcgtt ttttcttctt cacagttagc
     2821 tgtttcccat gaaccaccaa aagaaatcgc tggtgaggac tatcatgtat tttatccagt
     2881 aaccatcggg aaaaaccacg tggattttat ttattccaat cgttttgcat cagcagcaga
     2941 tcaggaatta tatgaaactt tgaataaaaa cggaagtata ttagtcaatg tatcagatta
     3001 cttgaatgaa gaaaatgaac agtttggcag agggataaaa ataaatctga attatcttaa
     3061 gaaatttcct ttgattgatg tctctggaag gttgatccaa ataaccgaaa aagaaacgca
     3121 ccctgttata ttgatccctg aacgatatag acatacagat ttaaaaaatg acctggcgca
     3181 aataacagaa tattatcaag aaatcagtga aaaagaaccg aaatttctat ttataaaaaa
     3241 caatcaaccc atttatacat ttattccttc tatcccctgg atcgaacatt atccggttgt
     3301 tgaaatactg acattgaaaa acagtatgta tttggaaaga aatattctca gcggagaaat
     3361 ctatccaccg ttaaagatca agataggctc tttgtcgaaa gaaaggttat ttgagctgat
     3421 tgaagaaagt cagttacgtg ataacttgca gttatcattt ccgtatactc agactgaaga
     3481 gatagctgtc aaagtgttaa gcagatcatt tcattacttg atacagatat ttttactcac
     3541 tttcttttct ttcttcttgt tgagctatgt catgttatgc atatattttg tctacaattg
     3601 tcggaaaatc gctatctttc gttcaagtgg ttattcgctc tttgaaacgt ataaagattt
     3661 cttcatgatg aacctcatca aatggggaac gacaagtgta atcttccttt ttcttataga
     3721 aagagagcct aaatatcttt ttaatatttt tttctttagc ttcattgatt ttattctttc
     3781 agttgttttt attttgtcta cagaaaagaa aagtcagtta ttgctgatga atggaggtta
     3841 acaattggag ccgttgatag tgataaaagg tgcgaaaaaa atcattcgaa acaaaacgat
     3901 tcttcatttg gatcgtttca ctgcggatgc tggggatctt gtgactgttg ttggagaaag
     3961 tggagcaggt aaaagtacgt tattgaatat attaggattg aatgatcagt tcagttcagg
     4021 acaattctat ttgttcgatc aagagacgaa acaattaact gggagagaga agcgcttgat
     4081 aaaaagaaat accatatctt ttctttttca agattttggg ttgattgaat atgaaacaat
     4141 cgattttaat ttagatattg gattgaagtt ttcgaattta aatgtttcag ataagcgaac
     4201 agcaaagtat gaggcattga aaaaagtaaa tataaacaag aagctaagta cgcctatcta
     4261 cacattatct ggtggtgaga agcaaagaat cgcctgtgcg agagtcttat tgaagaatag
     4321 caaaatcatt ttagccgatg aaccgactgg gtcattggat aaaagaaata aggataaagt
     4381 agttgaaatc ttatcgcaac aagcttccca aggtacgtta gttatcgtag tcacgcatga
     4441 tccatatctg accaaaatag gaaacaagaa gatcatgctt taaaaatcaa aaaggaaagg
     4501 atgtcgtttt atgaaaaaag cagttattat ttgtgcacta gggataggtt ttgttggagg
     4561 aaacgctatg tcatctgaag tggaagcaaa ttcagttcac ggtgaaatct attacaaatc
     4621 tttaaagcca ggagagattc attttgatgc taaaggtaat attattaagt caaatttacc
     4681 agtagaccgg gaaatttcta gaacagcaat taatcatagc tctggaagat gggtttatta
     4741 tagtgataca acttgtacga gaaaaacagg tcattctaac cactatagtc gtgtctataa
     4801 tcgtcacggt gcaaaagcga aagttggaaa ctctattcga tcagcaaatg caacaaaagg
     4861 agtatgggcc tatgcaagtg cgacaggaaa tagaactgac acattctcat gttggtacaa
     4921 tccaaccggt tggtattaag tatccaaaag tttatataaa atagaaaaaa catataatga
     4981 aacagcgtta aaagttattt ttggtagttt taatatattc atttttagtt gtataaaaat
     5041 atatcataat aaaaacaaca aaaaaatgtc tggaaaatcg aacataaaag tgtctgattt
     5101 gaatatccca gcagcggaag aagaaaaaca aaatcgataa catttgttct aagaattagt
     5161 tacgttaaat acggaacgca agatagactt gaaaaacggt aacaaaagaa gtagtatatt
     5221 aaggtgtgat gtgctacttg acatttattc tgctgagcga actttctgga ttgtgtagcc
     5281 ttatatctat tcaggacgta tcgagcagat tagtaaaggt gttcttgatg attttcatct
     5341 tgaacacctt tgtttttagc aattcgcaaa aaacgaaaag taggagaatt gataaatgaa
     5401 aaaaagattt ttagcacttg ccatcgtact aggcactggc ttattgtcag gctgtacgaa
     5461 tgccggtgaa aaaacagccg ttagttataa aggcggtaca atcagcgaac aagaagtaat
     5521 ggatagcttg aaaaaaatgc aaggagcaga ttccgctgtg caacagttga tcgtttatca
     5581 agtatttgaa gataaatatg gagatgacgt atcaaccaaa gaaatcgatt ctcaatatga
     5641 tcaaacaaag aaacaattgg gtgattcatt tgattcacaa ttgaaatctg caggttacac
     5701 agaacaaaca ttcaaagaca gtatcaaaca aagtctagcc tttcaagaag gtctaaagaa
     5761 acatatcaaa ttgaccgatg aagatttaaa aacggcttgg gaatctttcc atccagaagt
     5821 ggaagctcaa attatccaag tagcaagtga agatgatgca aaagatgtga agaaagctgc
     5881 tgataaggga gacgatttct ctaaattagc caaagacaag tcaactgata ctacgacaaa
     5941 agaagatggc ggaaaagtga aatttgattc cactactaca acggttccgg cagaagtcaa
     6001 agaagcagcc tttaagttga aagatggtca agtttctgat gtgatcactt ctaccaatgc
     6061 ttcaacttat actacggaat attatgtggt taaaatggtg aaaaaccaaa acaaaggcaa
     6121 cgacatggat aaatacaaaa aagaattgaa agaaattgca acagacacaa aattaagtga
     6181 cagtaccttc cagaataaag taattggcga agtattgaaa gacgctaatg taaaaatcaa
     6241 agacaaagac tttgaaaatg ttttgagtac attcacttca gattctagta cagcaagtag
     6301 taaataagcg gtataaataa aaaatatgaa ctatttaaat caataaaact gttcat
//
LOCUS       DAXWLB010000154         6278 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXWLB010000154 DAXWLB010000000
VERSION     DAXWLB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.157"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(48..1178)
                     /locus_tag="ACGE4H_002370"
     CDS             complement(48..1178)
                     /locus_tag="ACGE4H_002370"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFF8365754.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1238..1465)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1827..3029)
                     /locus_tag="ACGE4H_002371"
     CDS             complement(1827..3029)
                     /locus_tag="ACGE4H_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8365755.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3089..3292)
                     /locus_tag="ACGE4H_002372"
     CDS             complement(3089..3292)
                     /locus_tag="ACGE4H_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFF8365756.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3434..4501)
                     /locus_tag="ACGE4H_002373"
     CDS             complement(3434..4501)
                     /locus_tag="ACGE4H_002373"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFF8365757.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4559..4666)
                     /locus_tag="ACGE4H_002374"
     CDS             complement(4559..4666)
                     /locus_tag="ACGE4H_002374"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365758.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4829..5641
                     /locus_tag="ACGE4H_002375"
     CDS             4829..5641
                     /locus_tag="ACGE4H_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365759.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6015..6206)
                     /locus_tag="ACGE4H_002376"
     CDS             complement(6015..6206)
                     /locus_tag="ACGE4H_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365760.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1859 a         1294 c          991 g         2134 t
ORIGIN      
        1 gaaaagccga ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact
       61 tcttttgcga cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg
      121 tttccttctt ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt
      181 acaaattgtt gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga
      241 aacttagaag taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa
      301 ggagtaaaat ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt
      361 gaaaacaact cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga
      421 ttcccgcggc agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta
      481 ttgataatct ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt
      541 gcttctcttt tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa
      601 cggcaacctt tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca
      661 taaaaatcag atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg
      721 atctcttgtc gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca
      781 tctgtttcca ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg
      841 aaagataatt tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt
      901 tgattatatc cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca
      961 agatgccaaa atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt
     1021 tggtcttcca ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa
     1081 agtaagcctt cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt
     1141 ccgacatgat ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt
     1201 ttttgtaaaa aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg
     1261 taccaccttt tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct
     1321 tggtaacgaa agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt
     1381 ttcgacagtc aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt
     1441 ctactttcct tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa
     1501 agtcaatagg gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg
     1561 gaatacaata aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac
     1621 ttccttaaaa tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt
     1681 tcccatcaaa actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat
     1741 ttccgataca tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt
     1801 tgtcttatgg tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga
     1861 atagcgccct cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat
     1921 aagtgaccgt aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata
     1981 gatagcggtt gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga
     2041 agcttaatcg gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa
     2101 taaggtggcg taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt
     2161 tgcaagtcat accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga
     2221 taagcacatt tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat
     2281 gtcttctcaa tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca
     2341 agttctccaa tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg
     2401 gtttgaatct tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa
     2461 aactcaactt tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca
     2521 atcaccccct ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg
     2581 atataagatt ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc
     2641 tggcttagtt caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa
     2701 tgatagaaaa actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt
     2761 ttgacctgtc tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt
     2821 tgattggtag aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac
     2881 ttgtcataag caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc
     2941 tttcttccat tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta
     3001 tcttggtaaa tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc
     3061 tactttaata gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt
     3121 caattactcg ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa
     3181 tccttgcaag atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc
     3241 ccatctctat caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac
     3301 ctcctgattc tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga
     3361 tggttgtgtt atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca
     3421 gtactttgct tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat
     3481 aatactcaac ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga
     3541 tctgtcttgg attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg
     3601 tttgatcgac ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca
     3661 tagttttctc aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc
     3721 taataaaggc tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat
     3781 aaaaacaaat tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg
     3841 aaataatctt tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct
     3901 ttacttttgc ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat
     3961 aaaagcaaaa acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt
     4021 cattgtttat gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat
     4081 ggcctagtat taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta
     4141 aagtaaaatc aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt
     4201 gccaatttgt ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa
     4261 actccatcac agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat
     4321 tccacacgta ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa
     4381 tgggcatttc aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg
     4441 ttagataatc aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca
     4501 caagattcaa tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact
     4561 atctcgaaat ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag
     4621 aaactcgaat ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt
     4681 ctgctattta cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc
     4741 ctgtatttat tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt
     4801 taagaactta tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg
     4861 aattcggcag attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg
     4921 tgatttaccg cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga
     4981 aacccttcac caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga
     5041 tcaagagaat caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa
     5101 gattgaaggt ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat
     5161 ggatcgaatg attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt
     5221 tactttccaa tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa
     5281 ggaagcccag ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt
     5341 taccttctcc aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta
     5401 ttcctatttt gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc
     5461 aattaatcaa ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc
     5521 ttcttatcag tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc
     5581 attcgtgatg gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg
     5641 aggtgacaaa attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg
     5701 tagttgtcac agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct
     5761 gcagtccggc tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg
     5821 cgaaacccga cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact
     5881 gatattctca taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga
     5941 taacccgctg gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat
     6001 atacatctca acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt
     6061 accagtgcat tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt
     6121 ggcggaaatt tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt
     6181 attgcaatga aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta
     6241 tctacctctc aaattatttt tctctaataa gaaaaaca
//
LOCUS       DAXWLB010000155         6268 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXWLB010000155 DAXWLB010000000
VERSION     DAXWLB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.158"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            25..882
                     /locus_tag="ACGE4H_002377"
     CDS             25..882
                     /locus_tag="ACGE4H_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8365761.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            902..1462
                     /locus_tag="ACGE4H_002378"
     CDS             902..1462
                     /locus_tag="ACGE4H_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFF8365762.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2351..3118
                     /locus_tag="ACGE4H_002379"
     CDS             2351..3118
                     /locus_tag="ACGE4H_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFF8365763.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            3144..3689
                     /locus_tag="ACGE4H_002380"
     CDS             3144..3689
                     /locus_tag="ACGE4H_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365764.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYVHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            3690..4256
                     /locus_tag="ACGE4H_002381"
     CDS             3690..4256
                     /locus_tag="ACGE4H_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365765.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            4271..4519
                     /locus_tag="ACGE4H_002382"
     CDS             4271..4519
                     /locus_tag="ACGE4H_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365766.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANGFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4539..4778
                     /locus_tag="ACGE4H_002383"
     CDS             4539..4778
                     /locus_tag="ACGE4H_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365767.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            5149..5430
                     /locus_tag="ACGE4H_002384"
     CDS             5149..5430
                     /locus_tag="ACGE4H_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFF8365768.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            5420..5761
                     /locus_tag="ACGE4H_002385"
     CDS             5420..5761
                     /locus_tag="ACGE4H_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFF8365769.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKTGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2348 a          844 c         1264 g         1812 t
ORIGIN      
        1 tattatattt agatatcata atttatggaa gaaaaattaa ttaaaaagga tgagattata
       61 atggggttac tggttccgag agctaatact tatactatac ctttaacgaa acgccaatta
      121 attgctaaaa gatatcaaag gattactaga gccatcaata gagagttttg gaattctgaa
      181 agtgatactg ctcacagttt atacgtaggt tcttatggga gaggaacggc aattagtact
      241 agtgatatag atataatagt tgaattaccg atggcagaat ttgacagatt caaaaattat
      301 ttatcaaatg gcccgtctaa gttattacaa gttataaaaa acgcatttca ggaaatacta
      361 ccaaatagtg atattagagc tgatggacaa gttgttaaaa taaatttcca tgatggaatc
      421 aaatttgaaa tagttccagc ttttaatgaa aaagattatt ggggtgagtc aaaagggttt
      481 atttatccag attcaaacat gggagggaac tggaaagcta caaatcccaa aaaagagcaa
      541 gaagccatga aattaaagaa tacaaaaagt aataatttat tatatgctac gtgtaaacat
      601 tttcgacatg tacgagatac agaatttacg agttatcatc tttcaggaat agtaattgac
      661 agtttcgtat atgaggctat gggaaattgg aaattcgttg aaaataacag tgggggtcaa
      721 aacattagtt ctgtatctta tgagacagct ttattagaat actataactc acataaagtt
      781 atgggcggtt taaatttata ctctcctgga agcaatcaat ttgttaattc tgatagtagt
      841 atcatatgtt tagaaaaagt attaaaaaaa atagctttat aaagttttat ggaggaacaa
      901 catgggagac gaaaaaaagc gcttggaagt tcaattacgt gaagcatatg gacgagtaac
      961 gtatacttac accactcatt taaagcaagc cgataatatt atttctgaca ataaaatttt
     1021 aaggtgtctt caaattggtt tgtcagccct tactactgga ggggtactta gtaccattat
     1081 tactgataat agtgagttaa cctttgttgc tggattatgt tcaacaattt ctcttggatt
     1141 aaacttatac tttaagaatt ttgatcttgc tgaagaagct aaaaatcatc aaattgttgc
     1201 cgataaatta tggttgatca gagaagaata cgtaagctta ctaacagatt taaatcgttt
     1261 aagtgttgaa gatattgtga aaaaaagaga tcaattaatg aacgaaactc atgaagcata
     1321 taaaaatgcc cctaaaacaa atagtaaaag ctatgaatca gctcagaaag cattaaaatc
     1381 tgaagaagag caatttttta cagaagagga attggataag atgttaccag agcatcttag
     1441 aaaaaagagt tctaaatatt gaactctttt tttatgctta aaattctctt gtaatttttc
     1501 tattcatcta tatttaagaa gaaatatgat gatagaaaca tctcctaaag ccaccgcaag
     1561 ccgttctaag catagcttag cccgacattg ctagatatgc tcatctcacc atgaacgttt
     1621 cctaaagcca ccgcaagccg tcctagccat tgcctagtcc gacatcgcta gattagtgca
     1681 tctcgccata aaagttttct aaagccaccg caagccgttc taagcatagc ttagcccgac
     1741 attgctagat atgctcatct caccatgaac gtttcctaaa gccaccgcaa accgtcctag
     1801 ccatcgccta gtccgatttt gcttgatcgg ttccatttct gtttatttag tgggtcaaca
     1861 ccgtaatctt aaatagccat cgcctattta agattactag gtctgctttg aaggtaaaaa
     1921 gtctttcatt cttcaagact tttaggcttt caagctacgc ctgaaagcat tgaaattcat
     1981 cgctctttat ttggttttgc catttataat tccttacgcc cattatcttt ttcgcctgtc
     2041 ataccttgtc agaagtgttt agagcttatc tcatctttgg attaatacaa gtggcgtaaa
     2101 tttttttcta aacgcaagcg ttacgaaaat aatttactta aacacttgta ttaatccggc
     2161 agataagaac gcattctaat cactcccgac aaggcaagac aggcgaaaaa gataaataaa
     2221 tgaacgaaag gaagaaacat aaaatgcaaa accaaatagc gatgaagaaa aaaccacctc
     2281 cctctaaaac aatccaaaca aaaaagaatt ttgaaaaacc aaaaatcaaa aacagaaaga
     2341 agcgttcaac atgagagata gaattttaga agttaatcaa gaaatggtaa acaagttaac
     2401 aaatatcagt gggaactatg aaaagtacga tggtacttta cttgataact atatttttca
     2461 cgatacacaa aatatcagaa ttggcagaag taaaccgaga gcgttcattc tcatcaaaga
     2521 gaaatatttg aatgaatgga atagcaccta tgaaatggtt atgactgata actttgaaaa
     2581 ggtattggat ttcagaaatg ctttgttggg ttctgatatg ggaaatagca gtcttgaatc
     2641 tattgaagtt gaaaacaaga ccgagaatga aggtgaagga ttgattaaga atgtcttgaa
     2701 aatcgaacaa aaatatatca gtgaagatag aagtttttat agagtcacaa gctcggttgg
     2761 tctagcaaac tttgctacta acttaattgg aaacaatgct agtgaatctt tgctagtgat
     2821 gtgtctgaat acaaagaatg aggtcattgc ttatagcgaa gttttcaaag gtggaattaa
     2881 tcaatcagta gcgataccaa gagagatttt tcaactagct ttgctaaaca attcagcaaa
     2941 aatcgcagta tcacataatc atccgagcgg atatgaattt ccaagtgaaa atgacaaaag
     3001 ttttactacg aatttgaaaa acgcaggaaa aacaattggt attgaattgt tagaccatat
     3061 catcgtaaca agtaaaaaca caagctatta ctcatttcaa gaaaatggtt ctttataaaa
     3121 attagagaaa agaggaaaac aagatgggcg aaagaacaca agcgttaatt acagtcaaat
     3181 atggtaacag caaaacgaaa gcaagcgtgt cgattcatta tcagtggggg tatggtcgat
     3241 atatgctgat ggacgtattg aatcaaggaa tttatttaat caataaagca tacgacttac
     3301 gagataaatc aagagaagaa ttagcaaaag gaatctttga tagaagtaca ggagttatca
     3361 attttgttac ctatgaatct ggagtgagtg cttatgatga aacagggttt ggtgggcgct
     3421 atgaacctta tcaagaaacg attgaagatg tgatgaagtt ttcagataac aatgatggct
     3481 atgttcattt agagatggtg ttcgatgaaa atggatttag tctagatgat aaaaccagat
     3541 tgcgattgtt tgattttgag ggtcaacaga taggactaaa caagttttgt gaaagcggaa
     3601 aagagtttgc tacgaaagat tttattaagg gatacagatt attgctagaa aattatggaa
     3661 taaacctaga agaagagagc gtgaaataag tgtttgaaaa aagaaaagac cagttcccta
     3721 atttagaagc gaaaagtcaa aaagtgattg atggtgaaat gattgaatta ttccaaaaaa
     3781 gttatggaac catagaaact accgcaagtt gcaaatacta ctttcaagga aagagaaaca
     3841 cgtttaaagg ctttgccgat tctgatgaag aattggcaaa gaaaatcatt gattggcttg
     3901 tcagcagtga acacgtagca agggaagata ccgttagaac aacgattaat ggggaagtcc
     3961 taacaggtac tagatgtgtt tgttgtaatc atgaatttgt agaagatgaa ggttgctacc
     4021 gaatcaatga atatggtgat tttatttgcc agagttgcca cgagatagaa tttccaaccg
     4081 aaagaagttg gtttgaatac atgctacaag attatgagat ggaaaaagaa aatggtgaag
     4141 tagttttttg taccaaagag gaattgagta agagaagcga tgatgaaatt gaagattggt
     4201 gtgtgtggtt agtaggggca gatgattgcg ttgcatatta cacaacagca gaataggagt
     4261 gtgaatcagc atggaaggaa tggattttct agatcatgaa gatttggttg attttggtta
     4321 tacatggaaa ggaatggtag gtattagcag aagtcttgct aatggctttt acgaaagaaa
     4381 ttatgcagta tatgttcttt atgatgacga tacagaaagc cttgttgacg aggaatataa
     4441 attggattta gagaatgttt tatatggaat agaaaaggaa gatttagcaa aatatatttt
     4501 ttcatggttg ggtcaataag tgaaaggaag atagaaacat gacaaaattt aaaagtaagg
     4561 gtatgcaatg gtgttcttat tattatgttg gaaatgatga agatttattg agaaaaaccc
     4621 aacgaaaagg ttatccagat aagatattga aacaaatagg ttattacatg ccagaagaga
     4681 ggatagaaaa agaattttgt gaggttcttt ttgatagtga acagctatta gttttgttaa
     4741 cccaaaataa attgatttgc caatttgaaa ataattaaga tgaggtggtg aaccatgaat
     4801 gaagataaga tggtattaag tgaatatgct agattgagaa ctattaattc tttagcatct
     4861 catttaagtt gttctttcct aacaaaggtg ctaaaattaa tttacaaatg tgaagatttt
     4921 gtacaagaga agcacaaaga caagcctact gcgaataggc ttgtcttttt ttcattcatt
     4981 acgattccgt atgtgtgggt tagcgacaat ttgttcaatt accgtatgat atagcatctt
     5041 aatttgatta gattacttgc aatgtagttt attaagagta taatataatc ataaatgtgg
     5101 acaatttgat aaataatgag tgttttatac tcaaaaggag tggatgaaat gaaggtcaaa
     5161 aatccaactg aaaaaagcag aatccaagtc ggaatagaca agaatttaaa agagaacgca
     5221 gaaatgatat tggaagagct aggtttaaat cctacaacag caataacaat tctttacaaa
     5281 caagtagtcg ctagaggtga gtttccagta gagataaagc taagtgaaga ggagaagcaa
     5341 ggaatcagat tgcaacagct aactaaggac atgccagtag atgttctaga tacagatgaa
     5401 aagttagaag agtggttcga tgaagcctaa tgagatagta acggtatatg ttgcatttac
     5461 agataaaaat ggtggtaaaa gaagacctat tttggtggtt agtgataaag aagatagagt
     5521 agaattcttt ggaatcacaa gtcaatatga gaaaaaatca gaccaaataa agagggtgta
     5581 ttttcctatt gcagaatggg aaaaaacagg acttaaaaag caatcatgga ttgacgtagg
     5641 gtctttaaag gctattccca aaagccgaga gaacatatca ttcaaaaagg ttggtatgtt
     5701 atcagctagt gatacgaaaa agctaaatag gtttatcgag aaattacaaa gaaaagtcta
     5761 acaaggcttt tcttttttgt ttatatgtat attaagatat acgtttatac aaacgtattt
     5821 atgtatatga aaatatacgt aaatatatac gtgattataa acattaagaa aacgttaaga
     5881 attgttaaat aatgatattt agtaatatac gaattataat atatatttta tataaaatgt
     5941 atgtaaataa ataaaatgtc tgacggatat tgaatagcaa taaataacaa taattcagac
     6001 gaatgcggtg agtgaagtag cttgctacga acgaacaggc agaatgaaac ggcagtttca
     6061 ttagagcaag tcgcttgcga cgtagcgtta ccgctgatgt aactggcaag ttacatagag
     6121 agagtttgct tgcaaacgaa cgttacctat ttgaatagaa ataaaaaata acagattagt
     6181 gaattttgaa taggagccac aactcaacca accgcttgcg gtgggtgagt cccacggagg
     6241 aaaagacgaa cgaacggtcg cgtagcga
//
LOCUS       DAXWLB010000156         6247 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXWLB010000156 DAXWLB010000000
VERSION     DAXWLB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.159"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            342..2288
                     /locus_tag="ACGE4H_002386"
     CDS             342..2288
                     /locus_tag="ACGE4H_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365770.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQIFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2291..2746
                     /locus_tag="ACGE4H_002387"
     CDS             2291..2746
                     /locus_tag="ACGE4H_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365771.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2760..4088
                     /locus_tag="ACGE4H_002388"
     CDS             2760..4088
                     /locus_tag="ACGE4H_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFF8365772.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPVFNKIDADAETLTK
                     RFGILGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4122..4406
                     /locus_tag="ACGE4H_002389"
     CDS             4122..4406
                     /locus_tag="ACGE4H_002389"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8365773.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4408..4923
                     /locus_tag="ACGE4H_002390"
     CDS             4408..4923
                     /locus_tag="ACGE4H_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFF8365774.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4939..5601
                     /gene="rpe"
                     /locus_tag="ACGE4H_002391"
     CDS             4939..5601
                     /gene="rpe"
                     /locus_tag="ACGE4H_002391"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFF8365775.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5608..6180
                     /gene="hxlB"
                     /locus_tag="ACGE4H_002392"
     CDS             5608..6180
                     /gene="hxlB"
                     /locus_tag="ACGE4H_002392"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFF8365776.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
BASE COUNT         2117 a          878 c         1203 g         2049 t
ORIGIN      
        1 acaggagtct ggacttgact tgttcataga tgagcatttg taattaagtt atattataat
       61 aaaaaaatac gaattttagt gacgccatta tattgttttt atttttcaat tgtaatattt
      121 catattactt ccataataag gagtattttc tattggaata tcttagctct tttaaggatt
      181 tctccttttt tgtttctcat tttttaagat tatttggatt tcctttacaa caaacttcca
      241 caagtattat tgacatagat gtaattgagg gaagggggtt ccagtgctag aataaataat
      301 gaaaagaggt aacaaatgct agcgttaaga ggtgaacaag tttgattgaa agacatgtaa
      361 atttattaaa attattaagt cgacaagaag actttaaacc cgcttctttt tttagtgata
      421 aattggcagt gtcgaccaag accatttatc aagatattga attgttggaa gattatgtag
      481 ttaattacca agtaacaatt gagcgattac cttctttggg tatcaaactc ataggtgata
      541 ggaaaaacgt tgaacatttg atttcatcgg tagaaaatta tcttgaaaaa gatatttatt
      601 ctccagaaaa aagacgtgtc gagatcatta aagaagtttt gtttggtgat aagcctgttt
      661 ctttaactca tttgtctcaa atttttatgg ttagtaaaac atcgttatac aatgacctga
      721 gaattatcaa taaaattatt ggcagtggag aagttaagtt gcagtcatct gcagaaggaa
      781 ttagttttac tggtaatgaa caagcaattc aacgagcaat caagcaactt gtattctatt
      841 atgcgaatga catggaatca aaaacatttt ttcaagtttt aaatatgttg tttgattcaa
      901 aagttattag tgtcattcat gatttgctat tcaatgatta cagtgaactg actgaaaagg
      961 tttctgatta ctatgttcgg tcattgattt ctactttagt tatccaatgt aaccgtttac
     1021 tgaaaggtca tcatttaact gttgaagaag aattcctctt caatagcatt cgctatatgg
     1081 aaacgtacat tgtggcaaat ggtttaattg aagaattgaa ggcaaaattg aatcttccat
     1141 tttatgacgc agataaagaa tatctatgtc gtcaactatt tgcacatcgt gtgacaaata
     1201 atttaaaaac cagcaatgaa gagtacgcta tgagcgttca taaattgatt gaaagaatga
     1261 gtgagattga aaaaattgat ttgactcaaa atgatcgatt atatcaatcc ttattgtatc
     1321 atgtgccagc aatggtcctt cgtttaaaaa aaggtattaa aattcaaaat ccgttattag
     1381 aaaatatcaa atctcaatat tcagaactct ttagcatagt atggtacgcc atgtccatcc
     1441 ttgaagaaga atttgatgtg actttaacgg acgatgaagt ttccttaatt ttgattcatt
     1501 ttcagattgc tattgaagat cagtccaaag caaataatat tatcattatt tgccaatacg
     1561 gtatttcttc tgcgcaatta atttacaata aagttcgacg gtttttacca gcaaaagata
     1621 atgtagaaat atcaacttta gataagtttc atttagctga aattggggaa gttgatttga
     1681 ttatttcctc tctttccttt gatgcaggtg gtataccctt agtaagggta tctccattgg
     1741 taagtaaaac agattatatg aatattatgg aagcttatac taaatattta atagaagaac
     1801 cttcgcgatt tattcaaaat gaactgattc ctaaagcggt taaagttccc accatatcta
     1861 aatttgttca atcatcttta gtaaaaacag gagtacaggt ggaaagcaaa gaagaatgtt
     1921 tggatttgat gatttccgaa ctagaagcca attcattagt tactgaagag tttcgtgctt
     1981 ctatcttctc tagagagaaa atggggaata ctaatttaga aagtggtgtt gctttaccgc
     2041 atgcgaatcc atcaatggtc attgaatcca acatttttat tgtagcatta aaaaatgcga
     2101 ttaattgggg cgatcgaaaa gtcaaattag tcattatggt tagtttagct gaagaagatg
     2161 tatctgagat tagaggagta tatgaagaac tttaccaatt tattgggcaa aaagaaatgg
     2221 tcgatcgctt gttacaagtt catgatagcg aaaaactact aagaattttt caacaaaaag
     2281 gagaataaaa atgtatctag ataaagaatt atgtttattt caattagaag tgaaagatca
     2341 aaaagaattg tttcaagtaa tgtctgaaca attaaaaaac gctggttgcg tgaaagatag
     2401 tttcttagaa ggtatcacca accgtgaaca agaatttcca actggattag aagtaaatca
     2461 aattgggttt gctatcccac atacagattc cgcacatgta aatagctctc aaatctgttt
     2521 tgcctcatta aaggaaccac tggtattttc agatatgaca gataaatctc atgaaatacc
     2581 tgtacgttta gtctttatgt tggcaatgtc acaacctcat gaacaaattg atacactaca
     2641 aaacttagtt agcctatttc aaaatgaaga aaaagtaaat gaattacttg catgtaccac
     2701 gaaagaagca tttattgagt tagtacattc tgcaggagtt tattaggagg aatagagaga
     2761 tggatattgt attaaatgcc tttgaatggt ttattggatt agggtcaaac gtctttttac
     2821 cactgattat ttttgtaatc gggatattat ttggattaaa acctggtaaa gcatttgttt
     2881 ccggcgttac tgttggtatc ggttctattg gtttaggatt agttttaagt ttattatcag
     2941 atggtttagg aacagcaatt caacaaatgg gggaacaata cggtggtggc ctgaatatcc
     3001 tagatattgg cgtaggtgtt ggtggacctt tggcttactc tacatcattg ggaattttaa
     3061 tgattccaat ttcattaatt cttaacttca tatttgtggt tttgggttgg acaaaaacat
     3121 tgaatgttga tatttggaac ttttggttcg caattttcct aggtatgatt actcaatctg
     3181 ttacgggaaa cttttggttt ggcttaattg gcgcgattgt cgcagtaatt ttacaatggt
     3241 tcttagcaga tcgtacacaa aaaactgtta gcgaattttt cggctatcca ggaatctcta
     3301 tcacacatat gatgggatta tcaggagtac tatttgccaa accagtaaat tgggtttttg
     3361 atcgtattcc tgtatttaat aaaatagatg cagatgcaga aacattaaca aaacgtttcg
     3421 gtattttagg tgacacagtt gttattggcc taattatcgg tattgtagtg ggtattatgg
     3481 caggctatga cgtagcagga attggaaact taggaattat gactgcggca gtgatgaaga
     3541 ttatgcctaa aatggtagca atgtttatgg aaggtttaat gccaattgca gaagcggcga
     3601 aagaatttac gaataaaaag ttaaatggtc gccaagtaaa cattggaatg gatgcagcgt
     3661 taactgtggg tcatccaact attatgtcaa cttctctatt gcttgtacca atctcattat
     3721 tgctagcact aattcttcca ggaaataaag tattaccttt tggagattta gcattctttg
     3781 ctttcgccat ctgtttaatg attccttatt ttaaaggaaa tattgtgaga tcattagtag
     3841 gatgttcgtt gtatgttgtt acaacattat atatggcaac atggttggca ccaattatca
     3901 ctgatgtgtt tacattagca agtttcgatg tggggacagc aggattagta actatgttgt
     3961 tatgtggatt atggccagta gggttgtttg ttttattagc atcttacttt ggtgtaattg
     4021 gaataggtgg attaggagtt gttttagtag gtgcttctat ctacatgaac aaagtgaaga
     4081 aagcttaatc ataaaattaa aatacgaaag aagagatcaa tatgaaaaaa ttattagtta
     4141 tttgtggagc aggtcatgct acttctacaa ttgcagtagc aaaattatca aaatggttgg
     4201 aagaagaagg attaacagat caagtgaaaa tttatcaatc taaaattgct gatgagctaa
     4261 ataaaattga tgaatatgat gctgtagtaa gtacaacaat tgtccctgat gtaattaaag
     4321 ctaaagtaac gaatggcttg ggcttattaa caggtattgg tacagatgcc atttatgcag
     4381 atgtaaaaga aagattaggt gtttaacatg cgttttcatc atgacacttt gactattgaa
     4441 acagtttcag ggcgtccttc gtatcatgat attcgaaaag aacttttaga tacagttgat
     4501 acagcaggta ttgagaatgg aatgttagta atttcgagcc cgcatacaac ttgctcactc
     4561 ttttttgaag aaacgatgca tgacacaaac ttttttggtg atgattattt acatgtagac
     4621 atcaacaatg taatggaaac gattattcca aaaatgacga gtgaaaatca atacaacagt
     4681 ccggggccaa aacacattga ttttggaaca agattatctg atcccaatta ccctgcagaa
     4741 aaatgggtca tgctaaatac cgatgcacat ttacgttcat ctatttatgg aaccaactca
     4801 atgactttaa ttgttcaaga aggtaaactt ttattaggag aaatgggccg tgtctatttt
     4861 gtcgattggg atcaactaag agagcgaact agaacaatca atctcatgat tatgggagaa
     4921 taaacggggg aatagagaat gacaattgct ccatcattgt taaatgcacc tatcgcagaa
     4981 gttaaagagt tatttgaaat cttaaaagaa aagaatgttg attatttgca tattgatgta
     5041 atggatggct gttttgtacc agatatggct tttggaccaa gtgttattaa agatattcgt
     5101 ccactaacag acgttgtgtt tgatgttcat ttgatgattg atacaccaga aaaaattgtc
     5161 acaagatttg tagaagcagg agcagatatt gttaccttcc acattgaagc taccaaacac
     5221 gcaatgaagg tcattcagtt agtcaaggaa gcaggagcaa aagtagggat tacttttaat
     5281 cctgcaacac cagtcagttt tattgaacca gtattatctt tagtcgatca agtattagta
     5341 atgacaatta atccaggaac agagaataag cattttattc aagagacagt agttaaaatc
     5401 gaacagttag atgtaatacg caaacaaaat gattatacgt atgatattga agtagatggc
     5461 aagattgata atcaaacgat taaagtttgt tctaaagcag gggcggatat ttttgtatca
     5521 ggcggtttca tcttcaatgg gaaaaatacc ccgcaagaac aaattgataa actaaatcaa
     5581 tcattagagg aagtgaagta aaagatcatg accaataaag accaaaataa aaactattta
     5641 gaagcgatta tcttagaatt acaaaataac tcaaaacgta ttaattctgt agagttagaa
     5701 caatttagtg agaaaattgt tcaagctaaa cacatctttc tagctggtgc aggacgctct
     5761 ggaattgcca tgcaagcatt tacgaatcgc ttaatgcatt taggtttttc agtcagcctt
     5821 gtcggtgaaa ttagttcacc acattcgcaa cctggagatt tattaattat ctgttctggc
     5881 tcaggtgaaa caggtagttt aaaaaattta gcggccaaag caaatcaaag tggtgtaagc
     5941 attgctttag ttaccatgaa aaaagactcg tcgattggtc aattagcaga ttctgttctt
     6001 gtattaccag gtattaccaa agatgaaaat caacgagaag aagatacttt ttcacaacct
     6061 atgggttcaa gttttgaaca attagcattt ctcacctttg atagtttagt gttatcttta
     6121 atggataaaa caggtgaaag ctcagaaacg atgtttgctc gacatgcaga ttttgagtag
     6181 tagattttaa agatattatt tgtcaaatta agcgagacaa aactttccat ttacgtaact
     6241 caaaaaa
//
LOCUS       DAXWLB010000157         5960 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXWLB010000157 DAXWLB010000000
VERSION     DAXWLB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.160"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            12..857
                     /locus_tag="ACGE4H_002393"
     CDS             12..857
                     /locus_tag="ACGE4H_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8365777.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1048..2235
                     /locus_tag="ACGE4H_002394"
     CDS             1048..2235
                     /locus_tag="ACGE4H_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFF8365778.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2241..2597
                     /locus_tag="ACGE4H_002395"
     CDS             2241..2597
                     /locus_tag="ACGE4H_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFF8365779.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2599..2937
                     /locus_tag="ACGE4H_002396"
     CDS             2599..2937
                     /locus_tag="ACGE4H_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFF8365780.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3013..3269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3323..4366
                     /locus_tag="ACGE4H_002397"
     CDS             3323..4366
                     /locus_tag="ACGE4H_002397"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFF8365781.1"
                     /translation="MNGKSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGY
                     SGAGKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSR
                     TVLENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARAL
                     ANDPEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAV
                     MELGKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLT
                     FSERNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFI
                     KEQGVGIEVIHRG"
     gene            4359..5045
                     /locus_tag="ACGE4H_002398"
     CDS             4359..5045
                     /locus_tag="ACGE4H_002398"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFF8365782.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5069..5917
                     /locus_tag="ACGE4H_002399"
     CDS             5069..5917
                     /locus_tag="ACGE4H_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFF8365783.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         1922 a         1088 c         1344 g         1606 t
ORIGIN      
        1 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
       61 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
      121 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
      181 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
      241 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
      301 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
      361 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
      421 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
      481 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
      541 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
      601 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
      661 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
      721 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
      781 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
      841 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
      901 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
      961 tcccaaatgt ctatggaaag actttccatt atctaactgg accgctataa taatcagcag
     1021 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     1081 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     1141 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     1201 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     1261 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     1321 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     1381 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     1441 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     1501 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     1561 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     1621 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     1681 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     1741 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     1801 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     1861 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     1921 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     1981 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     2041 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     2101 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     2161 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     2221 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     2281 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     2341 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     2401 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     2461 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     2521 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     2581 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     2641 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     2701 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     2761 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     2821 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     2881 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     2941 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     3001 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     3061 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     3121 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     3181 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     3241 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     3301 tgaaaaatca taagagaaag atgtgaatgg caagtcatta atccaactaa cgaatgtcaa
     3361 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     3421 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     3481 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     3541 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     3601 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     3661 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     3721 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     3781 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     3841 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     3901 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     3961 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     4021 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     4081 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     4141 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     4201 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     4261 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     4321 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     4381 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     4441 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     4501 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     4561 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     4621 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     4681 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     4741 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     4801 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     4861 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     4921 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     4981 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     5041 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     5101 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     5161 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     5221 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     5281 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     5341 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     5401 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     5461 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     5521 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     5581 aacattcgat gatattgaag aaaaccctaa gaacttgaag ttcaaccata cgatcgaccc
     5641 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     5701 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     5761 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     5821 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     5881 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     5941 aataagtctg taatggctta
//
LOCUS       DAXWLB010000158         5930 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXWLB010000158 DAXWLB010000000
VERSION     DAXWLB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.161"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(55..879)
                     /locus_tag="ACGE4H_002400"
     CDS             complement(55..879)
                     /locus_tag="ACGE4H_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFF8365784.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(876..1631)
                     /locus_tag="ACGE4H_002401"
     CDS             complement(876..1631)
                     /locus_tag="ACGE4H_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFF8365785.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1644..3620)
                     /locus_tag="ACGE4H_002402"
     CDS             complement(1644..3620)
                     /locus_tag="ACGE4H_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8365786.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(3673..4344)
                     /locus_tag="ACGE4H_002403"
     CDS             complement(3673..4344)
                     /locus_tag="ACGE4H_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFF8365787.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4404..4613)
                     /locus_tag="ACGE4H_002404"
     CDS             complement(4404..4613)
                     /locus_tag="ACGE4H_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365788.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(4680..4919)
                     /locus_tag="ACGE4H_002405"
     CDS             complement(4680..4919)
                     /locus_tag="ACGE4H_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365789.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5329..>5930
                     /locus_tag="ACGE4H_002406"
     CDS             5329..>5930
                     /locus_tag="ACGE4H_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8365790.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT         1632 a         1226 c          912 g         2160 t
ORIGIN      
        1 ggctaaaaag gatagattaa aaactaatat tcttattatt ttcatcattt catcctaatt
       61 atcaaaaaat aattgagcaa aaaattccca acaattttca aaaaagttct tctttacacc
      121 agttgacaca acttttttaa tttcttcttt cggctctgat ttctctgatt tagggtataa
      181 ccaaacatcc gtcaaaaatt cacctttttc atttgaaaca ggtgtcaata aaacaatatc
      241 ttcactgttt gaaatgtttt cagggcttga ttcattaacg atatagtatg cttgtttttt
      301 gtttgcaggt acttcaatgg aaaattcaat cacgccttcc tgatcatcaa tcgttttggt
      361 ttctccttca gccacttttt tcaactggtc gtatgaaagt ttttttgctc gatctttcaa
      421 ttttgactca attatttcta cagattgatt tttctcatta tcatcttgtg acatttcttc
      481 taagatattc gtgatatcgt agacaataaa cttcgcgcca ttgatacctt cttgctgggc
      541 tacttgactg ccttgattgt tgatttgatt cgaagaaata attttttgaa tatgaatggc
      601 ttgcttgatc acttgcgtat cttctacgtc ttgcggcttt tctgaagtct ctgaagaact
      661 ttctgacgtt ggattcgttg attcagtaaa gcttatattt gaatctgccg tttcttctgt
      721 agtattctcc gaactgtccg tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt
      781 ggtactacta tcactgctgt cagcgaaaga cagttgcgaa taagctacag tagaaaagag
      841 agctgctaca caaacaaaca gccattttct cttattcatt ccgatctcct ccttattttt
      901 ttataaataa aaataatagg aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc
      961 aatgaaattg atttcctatt ttttgctttt ttctgtagtt ttaaatcttc tttactgaag
     1021 gaaactcgtt caccatacac taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc
     1081 attaacgtga tcctatcttg gtctttttca ggcataagat aatctaaata atttttttgt
     1141 aattcttctg ctttaacttc ttcaattcga taaatttgat aggcatgttg ttcttcattt
     1201 accttaataa agaatttttg cccttttttt actgaaggca aatcatcgaa aagattatct
     1261 ttataaagtc cactgtgccc cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg
     1321 ccagttcctt ccgtaatccc tacaccattc tctaaaattt tgtcggaagt tccgtcataa
     1381 attggtagtt ttaaagaaat acttggaatt gtaagaacac caacaggatc gcctaactct
     1441 attttcgcaa catccaaaga agaatctgtt ttttcaagat tttcaatggc taaaattctt
     1501 tgctgttgtt gaaatttcgc tacatttgtt tccacaactg gaattaaata gactgctact
     1561 gccgacagca accaaaaaaa tatcatcagt ttgtgcatga ctcttctttt tgggggctta
     1621 cgaataatca ttagctgatc cttctaggct ttcttcttaa agtaaacaac tgcagaaagt
     1681 ccccctgctc ctagcaacaa gcaagagatc aatacgtaag attggaaacc gttgctacca
     1741 gtcattggta cggaagcttt ttgattattt ttgatcgtga ttcggttttc ttctttgtat
     1801 gaatctttgt ctactttgaa agagatctct tttgttaatt tttgatagtt ttcaggagct
     1861 tttgtttctt ccagtgagta atctccttca gttaaaccag tgatttcaaa atgaccctct
     1921 ttatctgatt ttaggattgc tgcatttgct acatcgtcta cccaaactgc ttctttgaca
     1981 ccatctttat ctgtatcagt aagtccaccg tagtacttac cttctgcatt cttagctgca
     2041 aattctgcgc cttcaagcgg tgttgctttg atacctgttc caaataggcc gctatcgact
     2101 tttaagaatt ttgcgccata agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc
     2161 tgattaaatg gattattgtt ataaacgaaa tccacgctat ttgttaatgg atttgcgatt
     2221 tcagcactat cattcacaaa catgttataa gtcattttta gatcttgacc atgtaaattc
     2281 gcaactgtat cacttacttg tccattattt acgataaaat cgatcacaaa accactatca
     2341 gaattttcga ttttatagtc agtattttct tttaatacga ctgaaccatc attatttgtt
     2401 actttaatat ctgctttttc agcaacataa gaaagtcctt tacctgctgt atctccccat
     2461 ttaaatacac tgtaaggaga agtttcgctt ttcacaccgt ttactgtgat accgtctgcg
     2521 ccacctgcaa tgtctgttgg aatatgaatc gttccttgat aatttactgc gtcaccaatt
     2581 tggaaagaat gttcttgtcc tgttccattt gttactgttt tatcgatcac tggtgcttta
     2641 tagtttacgt atgaagcttt taagtcactt gtcacgccat cattcgtaat ttcaaaggtt
     2701 aatttattgt gcgcatcgtt gcgagcgtct gcgcctaata aatattgatc tgctgttggt
     2761 tcttgatctg atcccactac aacttccgaa ggaacttcca caaaatagta ttttcctaac
     2821 gtcaaatcag tcgcagtcag ctttccttga tcatccgata caagatcacc caattttgtt
     2881 ccttttccag gttcgccctt gtataactca aattttgccc cttttaaaag ggtttgtttg
     2941 ctttggtctg cgccatcgtc accaaatttc gttaattcaa aactcaactt agaaacaatg
     3001 ttcttaggat acaaatgaat attttttaaa aatcctgatc ctgttttatc tgtcattggc
     3061 gcaatcacaa ccattggttt tgctttttgt gtgactaatc cagcagcgga tttactttca
     3121 taaaccacat atacattccc tttggaattg acataggctg gttgatcagt aaaagtgatc
     3181 tcaccatttt catctacagc tgaagtaatt ttgttggttg cattcttgac gtactcactg
     3241 ttagatccct tgtcttctac atcttgtaca atctcgtcca ctttcgcgtt ggttagatca
     3301 gaatcttctc tcggtaatac ttgatccgta atatttccta acgtaaactc aacatcacca
     3361 taatctgcct tgttgtattt cgttacacct gtaggatatt gatcgtgcgt ataaccatcg
     3421 tttttgatcc cgtcaatatc aacattcaat tgcgttcctt gatcgtacat taatttatga
     3481 accgtgatat tgaccttctc tggtggtgtt ttttgcgtga catcttctgc atgtacttga
     3541 tacccctgaa agcagcctgc aatgattcct aacattccaa caagcattcc agcttttttt
     3601 acataccgat ttattgccat tttttactct cctcatttca tatttcaacg actcgcgttg
     3661 attaaaacct tgttattgta gctctccctg taccacgtat ctttcttttc cttgtgacgt
     3721 ataaacacaa gtaaacaacg ttaataaggg tttctctcct ttctttggca aagacaagaa
     3781 agagttctct tcaggaacgc catcgattaa tttaaagtac ccaggtacga taaatttacg
     3841 cgttaccttg taacgataga cgccatcgtt actaactaag tccatcacct cccctaatga
     3901 caacttttga atatcagaaa ataaaacacc tggcatttcc atgttatgac ctgctaggac
     3961 atagtttcct ttgcccattt cggcatcttc ataaaaataa gtagccgctc caagactcaa
     4021 agtgtattga tttgcccctt taaaaattgg caatgctaaa ccaagtgcag gaatctgaat
     4081 ttgtgcaaat acttgtttat tgacgcccat ttcgattgct tctttttgta tggacaacat
     4141 ttgccccaaa gacatatcat tattgacgtt cgttgcatcc tccttataac tctttgcatg
     4201 tgcattgatt tgatctacgg gcatcttttt ttctatattt tgcttaaaca cctctccttc
     4261 atgtttttgg tagctaacat agccaaacac actgcaaaag acgattaaag tgactcctaa
     4321 aaaaccaaat aaccattttt tcatccatct gcctcctttc agacaaaaca aaaaaacttt
     4381 ttctcatttc catagaaaaa gttttaaaaa tgaattctat aagttcgtct caactgttca
     4441 aagtttttca ttgaacctaa caaacaagct ttctcttttc ctgttaactg catatcattg
     4501 atatattgcg cgacagataa tcttttaagg tgtttttgct tcatcgtttt tagtaacttt
     4561 tcttccattt ctttcgacac tttttttctg ttttcgattt cttcataatt catgagtttt
     4621 cctaccttcc ttgttttttg caaaaataaa aaagcctatg atcacatacg ctctacactt
     4681 cagtcttggg ttatcttttt ctttcgttta attactggcc gtgctggttt caaatccttg
     4741 attccattat gggttctcca ataatcgaat tcttttcgca atctttcttt ttccatatct
     4801 gttcctttag ataaggcttt gaatacttct ttaggtgaat aaccacgagc taacagctgt
     4861 acataccttc cttttgtcaa agtatccata tccatcgcct ctaaaactgg tcgcgccatc
     4921 tactattcct cctcgtaaaa tatcatttgt ctaatagttt ttattcaaaa tgtttctaga
     4981 ttataaaatt tattgaataa cacattccta acggaatttg tttcgatatt gtgtgatttc
     5041 tcagagtaca atggatgtat ggaaaggatc caacgggaat ttgctatttc ctcctgtagc
     5101 gtttgtctac cctgtttagt ctgttacccg aaccactgtt atcactacca actcattagc
     5161 tgtttcgata atggactaga ctttcaaata gtagtttgga aggcagcttc tagctctttt
     5221 atcaccgtat tctattaggc gaggttgtgg ctatacagtg agtttctggg aaatcccaca
     5281 gtatcgaagc attttccaaa atttatatcg aaaggaggtc cttcttccat gaaattattt
     5341 gtcggtattg acgttagctc tgaaaaacta gatgtttgtt ttttaacaga cgacaatcaa
     5401 ttgtctatct tatctgaaat ctctgttgcc aatgacatcg aaggtgcttc attcatacga
     5461 gaaacaattc ttgaattcaa taacagctac cactttgatc aaattgtgat tggcatggag
     5521 tcaacgtcca tgtacagctt ccatccttcc atgttttttc atgaagatga gaaactaaag
     5581 aagctcaaca cacttgtaac gattgaaaat ccttttcggg tgaaacaatt tagtcgcatg
     5641 tttgatgaga ataaaactga tcgaaacgat gcattaagaa tcgctgattt cttgcgtatt
     5701 caacgattca caacttcccc tatcaaagaa gagaaataca tggccttaca gcgtttaaca
     5761 cgtactcgct atcaacttat taagcaattg attcgtacaa agcaacattt tcttgaaaat
     5821 ctaacgtaca aatgtaatac gcttgctcga gaaatgcgag acgaatcgac gagtcttttt
     5881 agtgcaaccc tcatttcact catgacggaa gatttcacat tggatgaact
//
LOCUS       DAXWLB010000159         5911 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXWLB010000159 DAXWLB010000000
VERSION     DAXWLB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.162"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(122..607)
                     /locus_tag="ACGE4H_002407"
     CDS             complement(122..607)
                     /locus_tag="ACGE4H_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365791.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(746..961)
                     /locus_tag="ACGE4H_002408"
     CDS             complement(746..961)
                     /locus_tag="ACGE4H_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFF8365792.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1185..1985
                     /locus_tag="ACGE4H_002409"
     CDS             1185..1985
                     /locus_tag="ACGE4H_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFF8365793.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2004..3872
                     /locus_tag="ACGE4H_002410"
     CDS             2004..3872
                     /locus_tag="ACGE4H_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8365794.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3865..4356
                     /locus_tag="ACGE4H_002411"
     CDS             3865..4356
                     /locus_tag="ACGE4H_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFF8365795.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4344..4898
                     /locus_tag="ACGE4H_002412"
     CDS             4344..4898
                     /locus_tag="ACGE4H_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFF8365796.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4916..5911
                     /locus_tag="ACGE4H_002413"
     CDS             4916..5911
                     /locus_tag="ACGE4H_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFF8365797.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2065 a          945 c         1192 g         1709 t
ORIGIN      
        1 aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg tataactttc
       61 tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata atatcatcta
      121 atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt gacactcgat
      181 aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa gctgtgaaca
      241 aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt tgtaattcgg
      301 aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga agagaaggta
      361 cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata cttgattggc
      421 tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta gaagtacttt
      481 tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca gaattctttg
      541 ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg taaatccctt
      601 tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac ttaaacaaag
      661 aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc atgaagataa
      721 taactttttt aataattata gctttctaat atctttcatc aacttttcta gtaatcattg
      781 ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg atcttcgcaa
      841 aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca
      901 acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct aaagttttca
      961 ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga aacattgctt
     1021 atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt tttgctcttc
     1081 cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt ttttagtgag
     1141 cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg gattggttgg
     1201 atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga agaagtatcg
     1261 tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact gaatgccgta
     1321 tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag
     1381 aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc aataatgcag
     1441 gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag tatgaattat
     1501 cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc
     1561 aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg
     1621 aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa gcagcgatga
     1681 acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg
     1741 ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa gaagctcttt
     1801 cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga acaagtacga
     1861 tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc tattatcttt
     1921 cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt aaaacaagag
     1981 gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa ttattgaaat
     2041 tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta
     2101 atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt attgcaaaaa
     2161 tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt tttgatgaga
     2221 tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa agaatcgctt
     2281 atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat ttgtctgaag
     2341 aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa gagatgatca
     2401 atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata ataggagatg
     2461 agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca gagacgaaaa
     2521 cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag acgaatgcta
     2581 gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat agaatctcta
     2641 gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa tatatccaag
     2701 aattcaatga actgatcaat cagttggaag aaatctatga actaactttg agccaatatg
     2761 aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg atgataagga
     2821 aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt cgacgattag
     2881 atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac ctctctttat
     2941 tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt gagatagaaa
     3001 aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta gaagctatct
     3061 tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg gagttagcat
     3121 ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat acgacaggaa
     3181 aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg gatatacaaa
     3241 taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat ttcgctgttt
     3301 ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat ttgactaatc
     3361 tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg
     3421 ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat
     3481 atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa gaatttgaaa
     3541 aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa tttgcttttc
     3601 ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta ataactgatc
     3661 aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata cctgaagaaa
     3721 tgacggctga agttgagaat gaattatttg agctatacga tatcctattt gaactcataa
     3781 aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt ttaaaactaa
     3841 tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt tttcgcagct
     3901 tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt caatcaaaca
     3961 tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg gaaaatccgt
     4021 tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga agtcagaaaa
     4081 atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc gggtgaagat
     4141 atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt gacacaagca
     4201 gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga aaaaagtgaa
     4261 caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa aagtacgatc
     4321 aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag ggatttatga
     4381 ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc gtaccagttg
     4441 tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa
     4501 cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct
     4561 tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc cttccagaat
     4621 actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc aatggtgtat
     4681 tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa gggatcgaaa
     4741 ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga ctgctgatga
     4801 actttattgg tggttggata actgatttta ctactggatt tgtatgcaga cagcaaggaa
     4861 tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt tttaaatgta
     4921 tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga ttattaggcc
     4981 gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc caatgatcgt
     5041 agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa catcaatacc
     5101 tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat gtggaattta
     5161 tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg gcccattagc
     5221 acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa tacagccact
     5281 aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata aaaagtacaa
     5341 atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact ggatagcaaa
     5401 aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa gagaagcagt
     5461 agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt tgattggtgt
     5521 gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt tagcaggaaa
     5581 tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat ctcctttgct
     5641 tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag agattgggaa
     5701 aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata ctcaaaatgc
     5761 atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga cagtagaagt
     5821 aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag ttatcattgc
     5881 ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAXWLB010000160         5825 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXWLB010000160 DAXWLB010000000
VERSION     DAXWLB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.163"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1451
                     /locus_tag="ACGE4H_002414"
     CDS             <1..1451
                     /locus_tag="ACGE4H_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365798.1"
                     /translation="YIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILHDLES
                     LAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIFTSTE
                     LADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYE
                     NKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKS
                     AETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKN
                     FVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEK
                     EQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSES
                     SVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLET
                     EKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1672..3435
                     /locus_tag="ACGE4H_002415"
     CDS             1672..3435
                     /locus_tag="ACGE4H_002415"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFF8365799.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3519..3977
                     /locus_tag="ACGE4H_002416"
     CDS             3519..3977
                     /locus_tag="ACGE4H_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFF8365800.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            4086..4769
                     /locus_tag="ACGE4H_002417"
     CDS             4086..4769
                     /locus_tag="ACGE4H_002417"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8365801.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4916..5530
                     /locus_tag="ACGE4H_002418"
     CDS             4916..5530
                     /locus_tag="ACGE4H_002418"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFF8365802.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5546..5752
                     /locus_tag="ACGE4H_002419"
     CDS             5546..5752
                     /locus_tag="ACGE4H_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365803.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         2087 a          910 c         1175 g         1653 t
ORIGIN      
        1 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
       61 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
      121 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
      181 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
      241 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
      301 gtacagaact ggctgatctt gaatttattt ctttgagcaa agtatataca atcaaaaaaa
      361 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
      421 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
      481 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
      541 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga ggaatagaga
      601 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
      661 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
      721 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
      781 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
      841 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
      901 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
      961 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     1021 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     1081 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     1141 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     1201 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     1261 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     1321 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     1381 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     1441 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     1501 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     1561 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     1621 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     1681 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     1741 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     1801 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     1861 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     1921 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     1981 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     2041 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     2101 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
     2161 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
     2221 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
     2281 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
     2341 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
     2401 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
     2461 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
     2521 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
     2581 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
     2641 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
     2701 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
     2761 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
     2821 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
     2881 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
     2941 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
     3001 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
     3061 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
     3121 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
     3181 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
     3241 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
     3301 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
     3361 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
     3421 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
     3481 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
     3541 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
     3601 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
     3661 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
     3721 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
     3781 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
     3841 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
     3901 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
     3961 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
     4021 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
     4081 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
     4141 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
     4201 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
     4261 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
     4321 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
     4381 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
     4441 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
     4501 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
     4561 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
     4621 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
     4681 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
     4741 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
     4801 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
     4861 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
     4921 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
     4981 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
     5041 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
     5101 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
     5161 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
     5221 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
     5281 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
     5341 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
     5401 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
     5461 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
     5521 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
     5581 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
     5641 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
     5701 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
     5761 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
     5821 tggtg
//
LOCUS       DAXWLB010000161         5762 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXWLB010000161 DAXWLB010000000
VERSION     DAXWLB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.164"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            196..921
                     /locus_tag="ACGE4H_002420"
     CDS             196..921
                     /locus_tag="ACGE4H_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365804.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACGE4H_002421"
     CDS             911..1420
                     /locus_tag="ACGE4H_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365805.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACGE4H_002422"
     CDS             complement(1490..2938)
                     /locus_tag="ACGE4H_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8365806.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACGE4H_002423"
     CDS             complement(2938..3654)
                     /locus_tag="ACGE4H_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFF8365807.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACGE4H_002424"
     CDS             complement(3635..3985)
                     /locus_tag="ACGE4H_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFF8365808.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACGE4H_002425"
     CDS             4129..4902
                     /locus_tag="ACGE4H_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8365809.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE4H_002426"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACGE4H_002426"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5571..>5762
                     /locus_tag="ACGE4H_002427"
     CDS             5571..>5762
                     /locus_tag="ACGE4H_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365810.1"
                     /translation="MAFKKTSFNIYVPSILPTLFLLYKKNISSYTLFEVNIMLMEGKT
                     QLWFKFDPSNRFIKDFYKVW"
BASE COUNT         1661 a         1135 c         1001 g         1965 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccattgt gagatttcgc cagatttcgc
     5461 tagatgctta aatccttatt ttataagggt ttgagcattt tttgttttcg ctgattttcg
     5521 ttaaatgaat gaaaatggca ttcaaaatgg cattcaaaat ggcattcaaa atggcattca
     5581 aaaaaacatc ttttaatata tatgttccat ccatattgcc aacgctattc ctgctttaca
     5641 aaaagaacat tagttcgtat actctttttg aggtgaacat tatgttgatg gaaggaaaga
     5701 cacaattatg gtttaaattt gatccttcga atagatttat caaagatttt tataaagtat
     5761 gg
//
LOCUS       DAXWLB010000162         5723 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXWLB010000162 DAXWLB010000000
VERSION     DAXWLB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.165"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..268)
                     /locus_tag="ACGE4H_002428"
     CDS             complement(<1..268)
                     /locus_tag="ACGE4H_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8365811.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVID"
     gene            complement(523..843)
                     /locus_tag="ACGE4H_002429"
     CDS             complement(523..843)
                     /locus_tag="ACGE4H_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365812.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(876..1994)
                     /locus_tag="ACGE4H_002430"
     CDS             complement(876..1994)
                     /locus_tag="ACGE4H_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365813.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(2023..2817)
                     /locus_tag="ACGE4H_002431"
     CDS             complement(2023..2817)
                     /locus_tag="ACGE4H_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365814.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2827..3702)
                     /locus_tag="ACGE4H_002432"
     CDS             complement(2827..3702)
                     /locus_tag="ACGE4H_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365815.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3919..4176)
                     /locus_tag="ACGE4H_002433"
     CDS             complement(3919..4176)
                     /locus_tag="ACGE4H_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365816.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(4179..4403)
                     /locus_tag="ACGE4H_002434"
     CDS             complement(4179..4403)
                     /locus_tag="ACGE4H_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365817.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(4576..5619)
                     /locus_tag="ACGE4H_002435"
     CDS             complement(4576..5619)
                     /locus_tag="ACGE4H_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8365818.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1580 a         1214 c          737 g         2192 t
ORIGIN      
        1 catcaataac tgtagcaata tattgagaga tagaaatatt atcttcactt ctatgtcgct
       61 ctataagatt atatatatca gggctgacat gaaacgtact tctctttttt gtttgccttt
      121 ttctaactgt tacgaccact tttggaactt cactatctcc attgatctct ttgtattttt
      181 caataaataa ttttctagct tgttctcgac tttttgccgg aatttcatct acaattttat
      241 cttctaaata aatatagtgt gttctcatac attcgtcctc tatcttcttt actctcaatc
      301 ttcatgatct tagttcttac cttgtttact cttaaacatt gcaaaaaaat ctagtcaaaa
      361 tcaaacttga aatcatcgtc tatcacaaca cccttaactt catctttaga tttcccaaag
      421 gaacccttat tttcaaatcc tttatttttg aatgatgatg gttatggaaa acttcctttg
      481 aaattctgac aagcataact agtcagaact gttttactta gattaagctt tcaaaagata
      541 ctgatgaaga actaagttct cagtaccttc ccattcttca ataaaaaata tttctccttc
      601 ttttacccac tctatagcta atttcttata atcaaataaa ccatatgtgt ttaagccttt
      661 gtctttcatt aaactcaaaa actgatcccc ttttaatctt tctgtatcat ccatcagaaa
      721 ttcaactaaa tctttatcca tgcgtgcaac tttttcgtct tttagtgcca ttgatttagc
      781 atagtcagtt gaatctttta gaataataac acctacattt ccttccacaa ttttcttctt
      841 catatttacc tcctaccatc ttttccaaag agtatttagt caaaatcaaa ctcgaaatca
      901 tcgtctatca caacaccctt aacttcatct ttagatttcc caaaggagcc cttattttca
      961 aatcctttgt ttttaaattc taaagctttt tcgacttctt ctttggatat tgtatgaact
     1021 tttttcttaa ctactccaag tttatacagc tcttcaaact ctggtacttc ggatctgaaa
     1081 tacaatccat cagtacgaat ttttaatagc ccatatgcgc tatgaacgta tttctgttca
     1141 tatctaacca tacgatctct aaatacgcct gctaccatgg aggaatacgc tcgattatac
     1201 atactgtatt tatcgaaggt gcttgtttct ccttctctta atgatttttg tccgatagca
     1261 ccaaatactt tcgttctaag atttttataa agcttcaacg tatcaggatc agtttttaat
     1321 ggctcgattc tttcaaaata ttctttgagt aatccttttt tcgtcaaaaa tgtgtggcta
     1381 tctaggactt ccatctcttt aatatcataa taatcaagga taagtgttag ctcaatctcc
     1441 gataaccaaa taggctcatt agctgttttg ggattttcgc cataatccgc aaacatcgac
     1501 tgtgattttg gattagcata tgttccttct ttggctgttc cttcaaaatt aaccaaaaag
     1561 aacatatacg tttttaaatt ttcatacggt cttacttttg gtctatttat cgctctcaaa
     1621 atagcttgat ctgtattata ttttgctttc aaataagtgt ttgacggcaa aggataattt
     1681 ctcatgatcc tcgaataggc ttcgttgaaa tcatattcga tcatctttgc tccttcctca
     1741 aacaaatcca agttatacat gctaccggag gtactggctc tataggctga acttggaaca
     1801 aagaaaggtg catcttccgg cttatcatag cccaaaaaat gtattcctgt ttgcttgttc
     1861 tgacggtaaa tgtccatcaa aagatatttg atcgtactaa aagaatctaa ttttcctctt
     1921 gtcatccacc ctaatccgac aagtctttta attttattct gcatttcagg tgtgtatatg
     1981 ctattgtttc ccatttatct gctcatctcc ggctttttta ttctagaccc tatatttgtt
     2041 cattacacca cttatctcgt ctacaaaggc tcccagatcg tgtgtaaagg catcatagta
     2101 atcttcaatc tcactcgatt cagttctccg gtttcttccg tttttatttc tgaatagata
     2161 catctgcgtt tctcttaaat cccttgcttt tttcgcaagt gctaataaat ccgtaaaatt
     2221 atcaataacc ataatgtcat tgatcataaa accttcttca tgaccatact tttcagccat
     2281 ctcgataaaa tcattaacat cgaacaactc caataattct gctttatatt tctctaacaa
     2341 tgtatttaaa tagcgatttc tcaattccca tgaactttca ccagttccgt attcaacctt
     2401 gatccctaaa ttcttagtgt agccttttgc tgtgtatcgt ccaatgaagt ctcccttatt
     2461 ccgattgcta tcttcttctt tgaaaaactc acgccaaatc ccctttttct tcttaggaat
     2521 tagacctctg ctcttgaaat catcatattt ttttgaagct tctcttcgtg tatctaagta
     2581 cctttgcttc agctctgtag ctgattcatt cgttccatat tcaacagtga tccttaagcg
     2641 cctgctataa ccttttgctg tatatcgtcc aacaaagtca ccgccacttc gatcactact
     2701 gacttcctta acaaattctc tccatatacc ttttttgtcc tttggtatta atcctcgtct
     2761 tttcatatct tgatattttt cagaagcttc ttttagagta ttcttttttg ctaccatata
     2821 ttccactcac tctttgttat ctagtatttt aataactggt ttcatttttg taatatcttc
     2881 tttttcagct atatacaact ccgttacggt aacgatctta gtatgattta gattaattac
     2941 tttcacttga tcgccaattt tgatttttcc tcgcaaccgt ttgggaataa accagttgta
     3001 taatcgtgat ttattattat ctacataagc ttttattgca ttttcaggaa acaggttatt
     3061 tagtctttca atttttttac ttttaaaaaa ttcattcgca aacttttcat caacaactaa
     3121 attaaataaa gtttcagaat ttacagcttc catatctatt cctttgtaga taggagaaag
     3181 caatttttta tattttgcca gtaatgactt tccagaatta ctaatcttaa tttggttact
     3241 taaatcttct tgataaagaa ccccatatcg aagtaacgcc ttaatataat gttttaatat
     3301 gccgttgata ccagaataaa tatccatgcc gttctcaata tagtagaaag tgtcgtctct
     3361 ccaaaacaat ttgaagctat tattcaaata aagtaacaag atcaatatat gtttgtcaga
     3421 aattctgaca aatctattac tttttagata ctcaccaatg gcttcactta actctttgaa
     3481 gccattgtat tttcccgata aaaatgtcgt aacattatct tctctctttt tcctatggtg
     3541 tgaacttacc ctgaaataat agagtctatt ctcatttaat attgaaaaat aaatactttc
     3601 agttgtttgt gagcattgta tatagttgaa atgatatcca tctggcatga cttttttaac
     3661 tgtattttgt actagctctg accacctttt gttagtagtc atatattttt ttccattaaa
     3721 ccaaacataa atatcttttt taaaaatttt gttccccccc tattgcgttt tctttgacat
     3781 taaagccctt tgttttcatc acattcgtat agccctagcc actcataacc tgatgctttt
     3841 gcgaagttta tttaaaagtg ttttcctttg taatctacct ttcccctcgt cctggtactt
     3901 ttcttggcga ctattaaact attcaacttt tttagaatca ttgtcagctt gaaatccttc
     3961 acgtttcatt atgtcttctg cttttccaat gctgattcct ttccaatcac gccatcctac
     4021 gcttccctta ttttgacgat aggctttaat agctgattcg gatatttgcc atcttgtagc
     4081 caactctttg gctgtgtact tatttgaaaa aagtagcttc tctatcagtg aaatatcaat
     4141 cattttattc tcactcctga aatcttcatt aatcatctct agctagctat atcttcaatc
     4201 aattctaata tgtcggataa accatcaaca gcttcttcaa gttgatcaac ggattcttct
     4261 gctttttcat atctttctga tccctgtaga tttttaggta tattttcttt gtactcttct
     4321 tcttgaatta cttctaactc atcctttagc tcggaaagtc gctcgcttat ttcttctaac
     4381 ctttttcttc ttctttggtt catcaaaatc acctcttcgc ataatctcta cgattttaat
     4441 ataccacttt tgtgtactac attcaatatt ttttgttata gattcgtatt acaaaaattt
     4501 cctctaaaaa aataagagcc tatttaccat ttcagtaatt aagctcttat ttcatttagt
     4561 tgataattct atcaattaac gtacagcaac cgccattcca gtcacattaa atgctctctc
     4621 atacgtgctt gcattgatcc attttgcgcc aaatacaggg tcaacaacat ggtatgcacc
     4681 atttctgaag ccatccacga gaactacatg tccattccat actgcacgac cccaaggcat
     4741 ttgtttgaac tctggcgtag catagttata agtaatccaa gttacaaccg gattaccatt
     4801 gcgaatttca ttcttgattc cattcgttcc tgaaccagtg atattgacgg cattaccgcc
     4861 aacgtttttt gcccatttaa ctactgggtc agccatcatg ccttgatacg ttccattaac
     4921 gttatgacgc cactctcctg agtaaccatg ataaggatta ttgtcattag cttttggcat
     4981 atagtttaca aaactggtta agctttgatt tgtggctaca cctttgtagt gtaaaccttc
     5041 taataaagaa gctccttcgc ataacattgt atttccttgc catacaacag gaacgttcaa
     5101 cactttttca gtgcttggag caggtgtagg atttggtacg ctgtttggca ttgcatacca
     5161 gcctgtacct tcaatcttcc aaccttgttt actaattacg ttcttttcat tttgatctaa
     5221 cgtgtagtga tgccaattca cgtatttgtt atataaacgt gttacaggaa ctctatttgc
     5281 tccgttgtag gaatagaacg caacaccttc gtacgtccat cctgtgtgtg aaatcttatc
     5341 tctttctccg gcatcaactg tgtaaaaatg ttcaccgtta taccgattat acaaacgata
     5401 aacaggagta ccacttgttg gagcttgcca tgcgacacct tcgtaatgcc atccataacg
     5461 tttcagattg tctctttctc caacgcttct agtgtataga tgctctccgc tattgggatt
     5521 atacatacga tacatggaaa tagagctttc tgctgaaact gaaggtgata ataatactag
     5581 ccccattgtg aatagaattc ctactaaaaa ttttttcatt tgattttacc ccttttcatt
     5641 ttttattgtc attattgcat gaaaattatt cgtaagattg ccaattacat aaacgattgt
     5701 tttaagatca taatcgttgt tgt
//
LOCUS       DAXWLB010000163         5689 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXWLB010000163 DAXWLB010000000
VERSION     DAXWLB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.166"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(488..1297)
                     /locus_tag="ACGE4H_002436"
     CDS             complement(488..1297)
                     /locus_tag="ACGE4H_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFF8365819.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACGE4H_002437"
     CDS             complement(1297..2043)
                     /locus_tag="ACGE4H_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFF8365820.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACGE4H_002438"
     CDS             complement(2061..2528)
                     /locus_tag="ACGE4H_002438"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFF8365821.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACGE4H_002439"
     CDS             complement(2528..2938)
                     /locus_tag="ACGE4H_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFF8365822.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACGE4H_002440"
     CDS             complement(2949..3962)
                     /locus_tag="ACGE4H_002440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8365823.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACGE4H_002441"
     CDS             4179..4913
                     /locus_tag="ACGE4H_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365824.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACGE4H_002442"
     CDS             4897..5607
                     /locus_tag="ACGE4H_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365825.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAXWLB010000164         5531 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXWLB010000164 DAXWLB010000000
VERSION     DAXWLB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.167"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            77..895
                     /gene="purR"
                     /locus_tag="ACGE4H_002443"
     CDS             77..895
                     /gene="purR"
                     /locus_tag="ACGE4H_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFF8365826.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1398..2771
                     /gene="glmU"
                     /locus_tag="ACGE4H_002444"
     CDS             1398..2771
                     /gene="glmU"
                     /locus_tag="ACGE4H_002444"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFF8365827.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2865..3836
                     /locus_tag="ACGE4H_002445"
     CDS             2865..3836
                     /locus_tag="ACGE4H_002445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFF8365828.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3842..3944
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4010..4579
                     /gene="thiT"
                     /locus_tag="ACGE4H_002446"
     CDS             4010..4579
                     /gene="thiT"
                     /locus_tag="ACGE4H_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFF8365829.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4660..5433)
                     /locus_tag="ACGE4H_002447"
     CDS             complement(4660..5433)
                     /locus_tag="ACGE4H_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365830.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1817 a          976 c         1164 g         1574 t
ORIGIN      
        1 cataaaatgc tcgaattttt gaagaaaaac ttatataatg taaaacgata aaatttctta
       61 aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca tgacacagta
      121 tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac gttatgaatc
      181 tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta aagaacgagg
      241 gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca tccctgaaat
      301 ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat cagaacaaga
      361 ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac ctgaactgct
      421 gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg atgctgtaat
      481 gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc tgaatgcgcc
      541 attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa gtgtcaacta
      601 tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa gtttgaaacg
      661 tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag tcaacggaat
      721 gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat ttgccgaatc
      781 aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg tcaaagatgt
      841 agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag aataagaggt
      901 tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
      961 ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatctagag
     1021 gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa ttgctcctca
     1081 tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg tttaatgaag
     1141 aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa aacagctttt
     1201 catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca ccggttacca
     1261 aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg ggataaaaga
     1321 aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc agcaaactat
     1381 atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca gggaaaggta
     1441 cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa cctatggtag
     1501 aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg atcgttggac
     1561 acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg ttacaagcag
     1621 aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa ggaaaaaaag
     1681 gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg ctgaacaatc
     1741 tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca caagcagaaa
     1801 acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa aaaatcgtgg
     1861 aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca ggaacttact
     1921 gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac aatgctcaag
     1981 gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat acagttgctg
     2041 cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc gcgttagcta
     2101 aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat ggcgtaagct
     2161 ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca gacacattga
     2221 tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt gtcatcgggt
     2281 cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa aactcagtca
     2341 ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat ttacgaccaa
     2401 aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa aatgctcaaa
     2461 tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg ctgggagaag
     2521 aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac aaacatcata
     2581 cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga cctgtcgagg
     2641 tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt ccagaatatg
     2701 cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa aaactccctt
     2761 atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga ctactattta
     2821 ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca aaacattact
     2881 ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca gagaaaattg
     2941 cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt gatggagaaa
     3001 ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt caatcaacga
     3061 gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca ctgaaacgag
     3121 caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt caagaccgta
     3181 aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt caaaaagctg
     3241 gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt ttctttgata
     3301 ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta gaaaaaggaa
     3361 tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca cgggcacgta
     3421 aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt ccaaaagcaa
     3481 atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt gtactgatcg
     3541 atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg aaagaagctg
     3601 gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct gctttacaaa
     3661 gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat cttccagaag
     3721 atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga gatgcaatca
     3781 aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat aaataataaa
     3841 taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa acccttcgta
     3901 cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac tattttcgaa
     3961 agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta tgggaaaaaa
     4021 tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt cgttgattcc
     4081 gatccagatc ggcagcagtt tttctatttc attagggcag attccattga ctctttttgc
     4141 gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca tcctacattt
     4201 tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt acccgattgc
     4261 ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag gctatgtaga
     4321 cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg gcgcactttc
     4381 cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg cactatgggg
     4441 gatgaattct tggctctttt ctttcgtaat gaatggtgcc agcgggttga gtacagctat
     4501 tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt ttgtgccaga
     4561 taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa tcatgtccta
     4621 gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg gcagataaga
     4681 aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga ttaaagtagc
     4741 gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat cttcaatcac
     4801 atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag aaaaaatgat
     4861 cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt tttcaacagg
     4921 tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga tcgaacttaa
     4981 tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc gattcagctg
     5041 atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg gcgaatgaag
     5101 atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg tcaattcttt
     5161 cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac gcctgttacg
     5221 agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca gccctgtatg
     5281 ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt gaggaaaatg
     5341 cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag cattacttgt
     5401 ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat ttcctcttaa
     5461 tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt taaaattcca
     5521 ttacaaaatc a
//
LOCUS       DAXWLB010000165         5473 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXWLB010000165 DAXWLB010000000
VERSION     DAXWLB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.168"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..202)
                     /locus_tag="ACGE4H_002448"
     CDS             complement(<1..202)
                     /locus_tag="ACGE4H_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365831.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRII"
     gene            295..644
                     /locus_tag="ACGE4H_002449"
                     /pseudo
     CDS             295..644
                     /locus_tag="ACGE4H_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(916..1206)
                     /locus_tag="ACGE4H_002450"
     CDS             complement(916..1206)
                     /locus_tag="ACGE4H_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365832.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1608..1844
                     /locus_tag="ACGE4H_002451"
     CDS             1608..1844
                     /locus_tag="ACGE4H_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365833.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1851..3803
                     /locus_tag="ACGE4H_002452"
     CDS             1851..3803
                     /locus_tag="ACGE4H_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8365834.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3875..4372)
                     /gene="dfrG"
                     /locus_tag="ACGE4H_002453"
     CDS             complement(3875..4372)
                     /gene="dfrG"
                     /locus_tag="ACGE4H_002453"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8365835.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4699..5391)
                     /locus_tag="ACGE4H_002454"
     CDS             complement(4699..5391)
                     /locus_tag="ACGE4H_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8365836.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1769 a          958 c          892 g         1854 t
ORIGIN      
        1 taataattct tgaactccat gtcttataat gttctatcga agcacccaaa tagccaagtt
       61 catctaatct acttgcataa actacgtcat ccgctctcat agtcggcata tacatattaa
      121 agagtaaaaa gataccccaa atccctatta gtaacaagat cagttctttg ttattcgtta
      181 tttttctatt atgtttgttc atttttatac tccttcttat atttcctaat agagtataac
      241 atagctttaa gcattactta aagataactt aaaagttatc ttttaaatac ttaattgtta
      301 aataccttat ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc
      361 cagaaaaatt tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata
      421 aactgccaaa gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt
      481 ttgtctcaaa aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag
      541 gcgaggcttc ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg
      601 gtactctcca ggaacgttta aaatcctaca attccactac ctgagactag taatgactag
      661 tctcttttta tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat
      721 ctgttcaatc tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca
      781 aatacttaaa tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg
      841 ggttcaaatc ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc
      901 tcagccgctt gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag
      961 acttttctca tattctacca caattttggg tttccctaac acatgatagg atctcacaac
     1021 aaattgcttt ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc
     1081 agctaggaag ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg
     1141 gatcaacggt ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt
     1201 ctccataacc ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa
     1261 ctctgtttta tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc
     1321 ctgcgaagat gaaggacatg tactaaaaaa aagaggcagc agtatagatt taagtagttc
     1381 ggttttggag tacaaaaagt gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat
     1441 ttgatactcg ttttttattg ttaaatcaac gtttgttgtt aatttacacc acaaaatgta
     1501 agttaaaatg tatataaaaa tgcgaacgtg aaataattat tgtaggaatg caaattggtg
     1561 tgttaaaatg gttacaattt actaagcaaa aaggggtgca ttttcctatg caatttcttg
     1621 agccaaaaaa taagaacgct aagtctgtgg attgggaaat atcagagcaa gtaagggtga
     1681 tagttaagca atacgctgaa tatgcagagc gtactgaaag cgaagcagtg gatgaattct
     1741 tattgaacat tttagacgat aagaagttta ttgaatggat tgccaacaag cgaagtaata
     1801 agcggattgt tgaaaaaatg ggtatcaaag atagagtggg ttgatagaca atgaccaagc
     1861 ttaaaagatt agccacgaag gaagatgaaa ttgtagatgt taaaattccg atttcaaacg
     1921 aagaattaaa agatagggct aaacaatatg atttattaac tccaaaacga tttgcaacaa
     1981 ggtataacaa aatgttgttc ttgccaacgt cttttaaatg gaacggttct gaatatccca
     2041 ttcaatataa ttactgcatc aatccgtttt gttgtaattt tgggaaagaa cagcacaaat
     2101 tcaaagatgt aaaagggaaa ccaagtcgat ataaaatgac tggtagttcc aaagataaag
     2161 gtcataaagg tatgtactgc aatgacaatc ctattggcag aggtgtatcg caaaactgta
     2221 cagtaacccc tttatctaat tggtctgttg ttgaagaaat taaacgcctt atagaaatca
     2281 atagcatcca agatgttgag ccagattatc aatttcataa agaaggctgc tcggaagaag
     2341 aatcaacccc attcaatgag ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat
     2401 ctcaacgcta tcaatgtaag gcgtgtaaga agttcactaa tgttctacca aaaagagaag
     2461 aaaccactac ttatcaccaa caaaagaaca ccattcttcc aatgttcgct aagatggttg
     2521 ttggaagggt ttcagtcagt cgtacttgtg atattttagg aattggagtt ggaacctact
     2581 atcataagct ggagtggctg tataggcgtt gtttggagtt tttagaacga tatgagacac
     2641 aaccccttca aacaaagaaa tttaacgaga tgtggcttaa tacggacaag atgcactact
     2701 acctaaataa cgtccgtaag aaaggacaag gttcaaagaa atatacgggg tttgaagacc
     2761 ttaatatgca aacgtatata gttgtatctg ctgaggtctt atcaagatat gtgttccgtt
     2821 ctgatgttgc ctatgattgg aatatttcaa tggatgaatt aaatgaagat acaagaaaat
     2881 tcaaagaaga ccatctcaat acttttagtc gtaaaaatga taggttagat tggtcttatt
     2941 atccacaaga accatccgct aacgatagcg aaaataggaa tgcttatctt cacgaattag
     3001 ggaaaattac taataggagt agatttgtcg atggtttaaa tgttgatgct ccttatacta
     3061 caactgctca ctattggtta attaaacaaa tggttaatgc cgacgaatgg cgaatgatta
     3121 gtgatgatga cttctctatt agaaacgcct tttatagagt attcacgaaa gaattaaggt
     3181 tgagcgatgc acatcatttt atttgccaag ttaacaagac gaaaagcaga aaacaatgct
     3241 taaaagaatt cggacaagca aaagcagaac tgctcgattg gggagatatc agaggattca
     3301 aaacaaagtt tctacggact ttggcatctc attatctaac tgaattacta acaagtcatc
     3361 aattccacga agaggctatt agtaaagatg gagaaagata tcgcaaatat gccgataacc
     3421 ctatcaaaca tccattagcc actaaggata aaggatttta ttcagttgat tgtagaacgg
     3481 atttatctgc tctggaacca aacgaaatcg caaagatgct attaaatgtt aatgaccatt
     3541 cgaccaatag ctttattcaa cagataagac gttatatatc atctcttgaa agaccattaa
     3601 caacagctcg tggtgataag aagagctata tttacgctaa cttcaatccg aagtatgctc
     3661 aatttgcgat tacaattcta agaacttact acaacttttg tcgccctttt aaatcagcgg
     3721 ataaaaaggt tttgacacct gctcaaagat tagggattac agataaacaa tttgattgga
     3781 aagatataat ttatttcaaa tgacaaaaag agcttggagt aatcccaagc tcttgtgctt
     3841 tatctttact gttcttcttc aattaacgca cccgttaact caataagttt tttctttcat
     3901 atacatgaaa atagtagtta tacggatttt tatcattctt tatccctttt tgggcaaata
     3961 cctcattcca ttcctcataa ttcacttctg gaaaaaaagt atctccttcg aattcatgat
     4021 gtatttttgt gatgtacatt ttctcaacat aagggaaaaa caaattataa atctgttctc
     4081 ctccgaaaat aaaaatttct tcttcgtttt tacataactc aaaaacatct tctattgaat
     4141 gaacaatttc acaaccatta aaggtaaacc ccttatctct cgtcagaata atatttcttc
     4201 tgtcaggtaa ggctcttccg attgattcaa ggttcttcct acctaatatt atcggatgtc
     4261 cctttgtagt atttttaaca tattcccagt ccttgggaat cctccaagga atgtcattct
     4321 ctttgccaat cactctattc ttatccatcg cagcaatcaa agaaactttc atttataaca
     4381 cagccttttt aactaattta ccatctaatt tcttatttct atcaaaaaac cttttctcat
     4441 tatcctgccc gttagttgaa taagcaattt tttctataag ttatattatc ataataccac
     4501 aaaatcacaa attttcattc aattatcatt ttcaattgca ttttataaat cattttgtat
     4561 gtaatttggt atgtaagtta aaatgaaaaa agtagtccat tttgaagtac attttgtcat
     4621 tttgtactcc aaaactgaac tacttagtat agattactgt tgcttcttta ctaatttagg
     4681 tttacttgtt aagaaagatt atttacgcaa atgttcttca acaatttgct gagatttatc
     4741 agctgtatct tccacttcat cttttgcttt ttctaacttt tgaatatatt gtttcaagct
     4801 tgagttttca gcagttgctt cgtctaattt cttttgtgta tctgttaact gagtttctac
     4861 atttgttttt tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg
     4921 taattctgaa agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg
     4981 attgatttca tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt
     5041 gctttgtagt tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc
     5101 cgattgattt ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc
     5161 ctttgaatct tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc
     5221 ttgctcttta tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat
     5281 atctgtctct ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat
     5341 tagacataga aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc
     5401 tccttaatcc aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt
     5461 ttttaaatta cat
//
LOCUS       DAXWLB010000166         5373 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXWLB010000166 DAXWLB010000000
VERSION     DAXWLB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.169"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(112..1617)
                     /locus_tag="ACGE4H_002455"
     CDS             complement(112..1617)
                     /locus_tag="ACGE4H_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8365837.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1614..2300)
                     /locus_tag="ACGE4H_002456"
     CDS             complement(1614..2300)
                     /locus_tag="ACGE4H_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8365838.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2493..3914)
                     /gene="gndA"
                     /locus_tag="ACGE4H_002457"
     CDS             complement(2493..3914)
                     /gene="gndA"
                     /locus_tag="ACGE4H_002457"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFF8365839.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            4141..>5373
                     /locus_tag="ACGE4H_002458"
     CDS             4141..>5373
                     /locus_tag="ACGE4H_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFF8365840.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT         1556 a         1110 c          950 g         1757 t
ORIGIN      
        1 atttaattgt tcgctagttt ttttataatg acatccctga atttctcact tgcttgtatt
       61 ttgtacctta ttctttgttg gatatataaa aaaacaagta aagcacatgc tttacttgtc
      121 ttctgccttt gcgataggaa taaagattct aaaaattgtc ccttgcccta ctacactttc
      181 tgctaagata cgtcctttat agttttcaac cagttgttta gcgatagaca accccagacc
      241 atttccaccc ttcgttctag ctcttgcttt atccactcga taaaaacgat tgaagatctt
      301 attaaggtct tctttgggta ttccttcgcc aaagtcctga atcgcgatct caaattcatt
      361 cagagtggaa gaaatagaga tatggacttc ttttcgtgta gttgaatact ttactgcgtt
      421 atccaatatg ataatgatta attgttcaaa gtgattacga taaatttgca gggtcacttc
      481 ttttgtcaaa tcatcatcta aagtgatcac atagtctgga tataatatct tgaaattatt
      541 ataaacttga taggtgactt cttttccttt agttgtttca ttcgcataat gaacatctac
      601 ttgttctgct cttgataagt ctagcatctc ttgaactaag cttttcatac ggctgatttc
      661 ttgcatgctg gcgcttaatg attcttctaa tatctcagga tcatctttcc cccaacgatt
      721 tagcagacta agatgccctt caataattgc aacaggtgtc cttaactcat gagatacgtc
      781 ttccacgaat tgctcttgtt gttcaatata caaacgcata cggtcaagca tctcattgaa
      841 tatttcagct aaatctgcca attcatcatt tgtgtcgatt tcaggcatgt ggatatctga
      901 ttgcggatct ttacgtatag tatccattgt atctctgagt accttgagtg gttttaagaa
      961 ataagaagat aaaaagaaac ctaaaatact acttaagatc aaagaaacaa cttctaaaac
     1021 aataagagtc aataatagct tatttcgaat atcatagaaa gaagataatt catagaagct
     1081 ttgcgcatag ccgatctttt ctcttgtacc tttagaatat ataggctgga tagataaaaa
     1141 gccagttttt ccttctagtg taacgatcgt tggaacttta cgtgtagtct gaatcaggtt
     1201 tcgactgttt tcatgggttt taaaaatcaa gttctcgtcc aaatcataaa cataaagatc
     1261 cattgatggc tgccctagct cagctataaa ggaatcgatc ttcattaagt ttccttcgac
     1321 cagtgaagta tgatctacga cctcatctcc gtcatttgtc gctttcgtca gattacgata
     1381 cgtatttact aatgtcagtt cttcatccga gttagccaaa cgagaagcta cttctgaaat
     1441 cgtacgctct acattgtttc tttcttttgc cacaatcaga ttgatagatg atttataggt
     1501 aatgacagcg aaaattgtaa acacaacgaa tatgaagaaa gaactcgcaa aggcccactt
     1561 gattgtcaag gagggccctt tcagttctct tttttccgat tttttcggct ttatcacgag
     1621 cgcatcacat aacctgttcc gcgtacagtt tggatatagc tttcctcccc gggaacgtca
     1681 attttgttgc gaagataacg aatatatacg tccactacgt ttgtttcaac ttctgtttcg
     1741 tatccccaca ctttatttag taaaacgtca cgtgctaata ccacgttcac gttttccatt
     1801 aaagtaagca gcaactcgta ttctcttttt gttagttcaa ttacttcatt tccgcgacgt
     1861 acgacacggt tttctttttc gatagtcaaa tcgcgataag taatagtagt ttgtttagcc
     1921 acatttttat cgccttcaat atcgattcga cgcaacaatg cacgaagacg tgctaataat
     1981 tcttcgatag caaatggttt gacgatgtag tcatctgctc cgtggtcaag tccagataca
     2041 cgatcgatca cagaatctct tgcagtcatc ataatgattg gtgtattttt gacttggcgt
     2101 acacgtctgc agacttctaa accgttcaat tctgggagca tcagatccaa caatatggca
     2161 tcccactcat tatttaatgc cgcatctaaa ccagttcggc cgttgtagtg gacttctgcg
     2221 ttataccctt catgcttcaa ctctagctcg acaaatcgtg ccaagttctt ttcatcttca
     2281 atgattagaa tattgctcat ttattatttt atcctttcta ttctactggc tttttaaaaa
     2341 ctctatcatt ataccgataa tatgcttaaa aatctatagt cattttatca tggatagcaa
     2401 ttttaaaaaa gagctgaagt ctactcataa caatcgactt cagctctttc gtcctctttt
     2461 tattttctca tttcctaaca gccgaaagac tattattctt catggtacca tgagtaatgg
     2521 aagattcctt ctttgtcgac acgctcatag gtatgtgcgc caaagtaatc gcgttgggct
     2581 tggattatat ttgcaggcag acgttctgct ctgtatgaat cgaagtaagc gatggctgat
     2641 gagaaagtcg gtacaggtac gccagcttgg acagccaagg caacgacgtc tctgactgat
     2701 tgctgatatt ttttcgtgat ttcaacgaag taatcatcta gcaataaatt ttcgatctca
     2761 ggatttttgt cataggcatc cgtaattttt tgcaagaatc gagctcggat gatacatcca
     2821 gcgcgccaga tttttgcgat ttcaccaaat ggcaagtccc aatcaaattc ttttgaagcg
     2881 acacgtaatt gagcaaatcc ttgggcataa ctcatgattt tactgaaata caacgcttct
     2941 cggatttttt cgaccaattc ttttttgtct cccgtgaact caaaatcgtt cgtacgagac
     3001 aaaatcttgc ttgcttgtac gcgttcttct ttataagcag aaatatagcg ggcaaataca
     3061 gattcagtga tcaatggaag tggcacacct aagtcaagcg cgctttggct tgtccattta
     3121 cctgttcctt tattcccagc tgcatcaagg atcacatcta ctactggttt acctgttcct
     3181 tcatcgtctt tacgtgtcag aatgtcagca gtgatttcta tcaaatagct gtctaattcg
     3241 ccttgattcc attctttaaa gatatcagcc atttcttcta ctgaaaggtt taaaatattc
     3301 ttcattaagt cataagattc agcaatcagt tgcatatctc catactcaat accgttgtgt
     3361 accattttca cataatgtcc ggctccattt gggccaatat atgtgacaca tggtgctccg
     3421 tcttctgctt ttgcagaaat ttgctctaag attggtgcaa ctagcgcata ggcttctttt
     3481 tgtccaccag gcatgattga aggtcctttt aaagctcctt cttcaccacc ggaaacgcct
     3541 gtaccgataa agttgatgcc agagttggct aactcttcat tacgacgcat cgtatcttta
     3601 aagaatgtat ttcctccgtc aatcaaaata tcgcctttat caaggtgagg aagtaactct
     3661 tggattgttg catctgttgc cggtcctgct ttgaccatta gcaagatccg acgtggtttc
     3721 tcgatagatt ctacaaattc ttcgattgta tatgttgctt tgaaattttt atctgggtgt
     3781 tcttctacta cagcggttgt tttagcccct gtacgattga ataaagccac tgaatagccg
     3841 cggctttcta tgttcaaggc tagattttta cccataacgg ccataccgac aacgccgatt
     3901 tgttgttttg acatatgaac tcctcctaca tacgggattt ctccctaatc tcttacatta
     3961 tagcttaaca cggctacatt gcaaactaaa gaacaacata ttttggctct ataatttatg
     4021 ggactgatga atcagaattg attcttcagc cccattttta ttttaggtat taaaaaacat
     4081 atcgttctct agtatgatta aatcacgaca aaataaacaa ctgagaggac gatatgctat
     4141 atgaatgatt ctatcaaaaa aatgctgcga ttaatagaga aagatttgat gattacagag
     4201 gtctcttacg agacctttca gaagaaaaag acgttgatcg tcgatgctgt tttctcacct
     4261 gctccccata cctgtagaaa ttgtggttct actgtggtag atggaaacgg gaaagtaatt
     4321 gtagtgaaaa atgggaagaa ggaaacgatt gtccgctttg aacaatacaa tcacatgcct
     4381 ttggttatgc gcttaaaaaa gcagcgctat acctgtaaaa actgtcgaac ccattggacg
     4441 gctcaaagtt attttgttca acccagacat tcaatcgcga atcatgtcag atataaaatt
     4501 gcttctttac tgactgaaaa agtatcttta tcttttattg cgaaaaattg tcaggtatct
     4561 ttgaccaccg ttattcgtac gttgaaagag tttaaaagct acttaccaaa gcaatctaag
     4621 aggattctcc ccagagtatt gatggttgat gaatttcgtt cgcacgcttc catagaagat
     4681 aaaatgagct ttatttgcgc agatggcgaa acaggacaat taattgatgt cttgcctacg
     4741 cgtaaattgc ctcgattaac aagctatttc ttaggctgcg ctaatcctga agaagtaaaa
     4801 tttttagtca cagacatgaa cgccgcctac ttccagctta ccaaacgtat tctgccaaat
     4861 gcgaaagtag tgattgatcg gtttcatatt atcaaacaca tgaatcaagc ctttaatgag
     4921 ttgcgtatcc gtgaaatgaa tgaacttcgt aaagtaggac agaaaagtca ggcagaaaaa
     4981 ctgaaaaaga actggcgctt tttgctaaaa aatcgtgcaa acatcaacca ttatgaatac
     5041 aaaacatgga aaagttttcg agcaccaaaa tacccatttc ttacggaagc gatgatgatt
     5101 gatcgattgc ttggcttttc tacgtccctg aaggaggcgt atccctattt tcatgaatta
     5161 gttgaagcct ttcgagacaa agaccctgac ttatttttct ccttattggc agaacttccc
     5221 gaaacgttag atgacggctt tcgggaaaag ctccaaaacc ttctgaccta tgaagaaggc
     5281 atcaccaacg cgatgatcta tccttacacc aatggaaaaa tagaagcgaa gaatacccat
     5341 ataaagacaa tgaaacgagt atcctacgga ttt
//
LOCUS       DAXWLB010000167         5341 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXWLB010000167 DAXWLB010000000
VERSION     DAXWLB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.170"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            212..310
                     /locus_tag="ACGE4H_002459"
                     /pseudo
     CDS             212..310
                     /locus_tag="ACGE4H_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(533..763)
                     /locus_tag="ACGE4H_002460"
     CDS             complement(533..763)
                     /locus_tag="ACGE4H_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFF8365841.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(993..1811)
                     /locus_tag="ACGE4H_002461"
     CDS             complement(993..1811)
                     /locus_tag="ACGE4H_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFF8365842.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1972..2661
                     /locus_tag="ACGE4H_002462"
     CDS             1972..2661
                     /locus_tag="ACGE4H_002462"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFF8365843.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2675..4177
                     /locus_tag="ACGE4H_002463"
     CDS             2675..4177
                     /locus_tag="ACGE4H_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFF8365844.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4190..4666
                     /locus_tag="ACGE4H_002464"
     CDS             4190..4666
                     /locus_tag="ACGE4H_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFF8365845.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4825..>5341)
                     /locus_tag="ACGE4H_002465"
     CDS             complement(4825..>5341)
                     /locus_tag="ACGE4H_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFF8365846.1"
                     /translation="RPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIK
                     PNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP
                     SQRPLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLS
                     YVQEAFYSSLI"
BASE COUNT         1776 a          788 c          959 g         1818 t
ORIGIN      
        1 ttaaggggca gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg
       61 gtgtatgggt tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg
      121 gtgatgaaat tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca
      181 aaacacaaat tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact
      241 ggattatcga acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa
      301 cttaatttga caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca
      361 ataggcagat gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat
      421 caaaaggatc ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca
      481 gtttgtctca taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt
      541 caacgttgtt cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta
      601 gaacaccctt tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc
      661 gtttagattc agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct
      721 taataaattt atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga
      781 taatgagcta cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt
      841 agataaaatc aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat
      901 tagtaaaaaa taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta
      961 aaaaccgtta ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag
     1021 cgcaaaactt tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga
     1081 actaatagat cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga
     1141 aattcttcaa tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata
     1201 tagttagtta tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa
     1261 gtatctttcg ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa
     1321 gaagcgactt gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga
     1381 agaaacatat tttctaaatc aagactagta tttccactag atcccactcc aaatatatat
     1441 atgttttttg attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc
     1501 ctaatagtgg aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca
     1561 ctagtcggat gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca
     1621 gaaaaaccag aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt
     1681 gtgtttttct taatatcact catcgtacta taaataatag ttttcccaga atttaaaata
     1741 aaatcagcga cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag
     1801 atataactca tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat
     1861 aaaaaaaact cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt
     1921 gtttttggag ttattatcca aaaataataa aacattggag cgtttaggat aatggataaa
     1981 aagaggtttt tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt
     2041 gaaccatttt acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg
     2101 gctggagcag ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt
     2161 gtatcattac caatgattgg aattattaaa agagattatc caccagaatc tccattcatt
     2221 actgccacta tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt
     2281 gattgcactc ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata
     2341 aaagaaaaat atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa
     2401 aatgcatata aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa
     2461 agtaaaaaac aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt
     2521 tgtgtaattg ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata
     2581 ggagttgcag gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga
     2641 tttgttaaag cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg
     2701 acgcgctttc atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg
     2761 aggagcgtta acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt
     2821 gcatacaatt ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact
     2881 aatttttgca attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt
     2941 agctggtgga gtagcttatt tagtatatac tgcaactatt agtgggtttc ttgaactttt
     3001 ttcagcaaaa gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac
     3061 tgttgcattt ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt
     3121 tggaggatca agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt
     3181 cttttattta atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc
     3241 ccaaatgggt agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt
     3301 tggactacac cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac
     3361 tgtagctgga tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc
     3421 aaaccacact ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat
     3481 gatgtttggg ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg
     3541 taaaaagaat ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac
     3601 tgaaccagtt gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt
     3661 cttagatgga ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt
     3721 ttcaggtggt ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa
     3781 ttggttaaaa ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac
     3841 attctttatc aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca
     3901 ttctgaaaaa gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga
     3961 agctcttggt ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt
     4021 tgctgttacg aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc
     4081 tgtcttcgaa gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag
     4141 tcaagaaatc aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat
     4201 tttcaaaaag aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat
     4261 cacagaagta aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat
     4321 acctgatggt tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac
     4381 taaacatgct attaatataa aaagcaactc aggcattgat tatttgattc atattggaat
     4441 tgatacagtt gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt
     4501 tactcctgaa acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga
     4561 tacttctgtt atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa
     4621 tggtcgaatt gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa
     4681 aaagctataa aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac
     4741 tgttttcttt ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga
     4801 tccgtaattt aaagttatga tttgtcaaat taagctagaa taaaatgctt cttgtacata
     4861 gctcaaaaat atttcgattg gtgttctgta attcaatgat tttcttggaa tatgattacg
     4921 ttgattgctg acactggaaa taaatgtctg attcacttct ctaaaatcca ttgatttcgg
     4981 cagtccatta cgacgcagaa tcccgttaga attctcgttt aatgggcgtt gagaaggtgt
     5041 tccagggtcc gcaaaatata tatcaatatc atgttggtta ctaatggctt tccagttaga
     5101 aaattctttt ccacagtcaa acgtaataga tttaaagaag tttttaggga agcgagaaaa
     5161 ccattgatta agggcagttt caatatctaa tgccttacgg ccgttgggtt taatcgtgat
     5221 aatgacttta gataatcttt caactaaagt aatgacggca cttttatgat gaatgccaac
     5281 gatcgtatca ccttcaaggt gaccaaactc agaattgaaa tcaggataat tatcaggtcg
     5341 a
//
LOCUS       DAXWLB010000168         5310 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXWLB010000168 DAXWLB010000000
VERSION     DAXWLB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.171"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(60..1199)
                     /locus_tag="ACGE4H_002466"
     CDS             complement(60..1199)
                     /locus_tag="ACGE4H_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8365847.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(1508..2221)
                     /locus_tag="ACGE4H_002467"
     CDS             complement(1508..2221)
                     /locus_tag="ACGE4H_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFF8365848.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(2475..3176)
                     /gene="nagB"
                     /locus_tag="ACGE4H_002468"
     CDS             complement(2475..3176)
                     /gene="nagB"
                     /locus_tag="ACGE4H_002468"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFF8365849.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            3410..4942
                     /locus_tag="ACGE4H_002469"
                     /pseudo
     CDS             3410..4942
                     /locus_tag="ACGE4H_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(5133..>5310)
                     /locus_tag="ACGE4H_002470"
     CDS             complement(5133..>5310)
                     /locus_tag="ACGE4H_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365850.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1748 a         1079 c          892 g         1591 t
ORIGIN      
        1 aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct tttttatgct
       61 tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac atgttccatt
      121 cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac tgcatagaca
      181 ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat caataccaag
      241 atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt gatcaaattt
      301 ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg aaatggagag
      361 ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac atacgggata
      421 atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc gaatattctc
      481 attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact gctgatatag
      541 gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg aatcgctttc
      601 ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat aaatagcaaa
      661 ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat gatttttgag
      721 acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga taaatttgct
      781 gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc caattcattt
      841 aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg aattgtctgt
      901 tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat tacgaccaaa
      961 aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa aacaacgatt
     1021 ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaat tgccgaaagc
     1081 attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac gccgatgatc
     1141 gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg tgattgcata
     1201 catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag ttacgcgctg
     1261 agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga gtagacgagt
     1321 acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc cctttttcca
     1381 aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg aaaaatataa
     1441 ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt ttacaacctc
     1501 agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg tgtacggaca
     1561 actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta tagatatttt
     1621 tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg attacgatat
     1681 ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg ataaattgga
     1741 tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg agtgcaatag
     1801 caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg gcgatcccta
     1861 atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat tgtttcgtga
     1921 cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca atcagccttg
     1981 cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct cttaatgcac
     2041 cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa actaaaatat
     2101 gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt gctccgccaa
     2161 atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc atatcattca
     2221 tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa aaagtgcaat
     2281 ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct ttttatgaat
     2341 aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa tatggggagc
     2401 tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca acctctttag
     2461 cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac aacaacattg
     2521 tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc aatcatccct
     2581 ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt acttttcata
     2641 attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt atcgaagaat
     2701 cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt tccgtcaaat
     2761 gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa gatttggata
     2821 tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc cagatcttct
     2881 gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa ttggacatcc
     2941 atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc atctaagttt
     3001 acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata taaagtaact
     3061 ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc ttctttgatg
     3121 atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat ttccatagtg
     3181 attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct ttttacaata
     3241 attagtatag accaaattgc atttttttca ttttcttgac aataaatagg aatccgtgtt
     3301 aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa gcaagtgatt
     3361 cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta tgagtatact
     3421 gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag gtgtcgatct
     3481 gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg gtaaaagtac
     3541 attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc attggcaaaa
     3601 aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat taacaatcga
     3661 agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa ttgcttttct
     3721 ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg ggaaactcca
     3781 aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg agatgagcag
     3841 cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa gtggtggaca
     3901 acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc tgatcttaga
     3961 tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt ttcttcagga
     4021 ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc agatcaccac
     4081 ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc taaaacaagc
     4141 attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg ctcaacaaaa
     4201 acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc gatcaacgat
     4261 ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc cagcttcagc
     4321 aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag cagtggaaac
     4381 aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt tgaccatccg
     4441 ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta ctttattaaa
     4501 aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag ataacacaaa
     4561 aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac cactacaata
     4621 tttaagcagt taatttccaa aagcgactat caaagaatta cgtcgccaac tagctaaagc
     4681 agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg gggaacaaac
     4741 taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc tcgatgaacc
     4801 gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac agaaatttcc
     4861 aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg tgcaacgagt
     4921 cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg agctgtgaca
     4981 aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg accttcggta
     5041 ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc ttattacttg
     5101 atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag atttctcttt
     5161 cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag cccgctaacc
     5221 gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga accagccgca
     5281 cctccaataa agtacataaa cataaataca
//
LOCUS       DAXWLB010000169         5246 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXWLB010000169 DAXWLB010000000
VERSION     DAXWLB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.172"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1567
                     /locus_tag="ACGE4H_002471"
     CDS             <1..1567
                     /locus_tag="ACGE4H_002471"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8365851.1"
                     /translation="SAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLEQVVLLADESL
                     EEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQL
                     NDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHD
                     GDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMG
                     SGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKD
                     WPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHL
                     KETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLK
                     KNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKE
                     NDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEM
                     WLQGALEVFKHEQ"
     gene            1557..2756
                     /locus_tag="ACGE4H_002472"
     CDS             1557..2756
                     /locus_tag="ACGE4H_002472"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFF8365852.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            2769..>5246
                     /locus_tag="ACGE4H_002473"
     CDS             2769..>5246
                     /locus_tag="ACGE4H_002473"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8365853.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNR"
BASE COUNT         1843 a          893 c         1096 g         1414 t
ORIGIN      
        1 tagtgcggca gataatctcc gtagtaaaat ggatgcgtca gaatataaaa attatttatt
       61 gggactgatt ttctataaat atttatccga tcgattgttg gaacaagtgg ttcttttagc
      121 agatgaatcc ttagaggaat atgacacagt aagcaaacaa acaatgttgt atcgtgaatt
      181 actttcagat gaagaatcaa aagaagatct gattgccaca atcgttgata ttttgggata
      241 tgcaattgca ccagagtatc ttttcaacgt cttagcggat caagcaaaac aagcaacgtt
      301 ccaattgaac gatctaaaca aagcctttgt gcaattggca tctacctaca atcaatttaa
      361 tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg agcaacaacg
      421 aaatgtgacg attaccgaag tcatcaagaa attgaatgat gtagatgttt tagaacacga
      481 tggggacgtg atcggggatg cgtatgagtt tctgatcagt caatttgctt cagaagcagg
      541 gaagaaagcc ggagaatttt acacacctca tatggtttct gatatgatgg cgcaaatcgt
      601 aacgctcgat caaaaggaac gacgattctt tagtgttttt gatccaacga tgggttcagg
      661 ttcattgatg ttaaacgtac gtaactactt aacacaccca gataacgtta aatatcatgg
      721 gcaagaacta aatacaacga catacaatct agcaaaaatg aacttgattt tacatggtgt
      781 cgacgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggact ggccaacaga
      841 tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta attggtcagc
      901 agatacgact ttcttagatg attctcggtt taatcgttat gggaaattag cgccaaaatc
      961 caaagcagat tttgcgtttc ttcttcatgg gttctatcat ttgaaggaaa caggtaccat
     1021 ggccatcgtc ttaccacacg gagttttatt ccgtggcgct gcagaagggg tcatccgtca
     1081 aaaattacta gaagatggca gtatttatgc tgtgatcggt atgcctgcga acttgttctt
     1141 tgggacatcg attccaacga cggtgattgt gttgaagaag aatcgtcaaa ctcgagatgt
     1201 attattcatt gatgctagtc gtgaatttgt taaagggaaa aatcaaaata aactatcgga
     1261 agaaaacatt caaaaaatcc tcgaaaccta cgctgaaaga aaagatgtcg agaaatacgc
     1321 tcatttggca acctttgatg aaatcaaaga aaatgactat aacttaaata tcccacgcta
     1381 tgtagatact ttcgaagaag aagaaccaat tgatatggtt catgtcggaa atgacatcaa
     1441 gaagatacga caagaacagc aagtgctaga aaaagaactt ttagaagcta tctcttccct
     1501 gcaaacaaca cctgaaaacg aaatgtggtt acaaggtgcg ctggaggtgt tcaaacatga
     1561 gcaatgatac acgaccagaa atccgtttcc caggatttac ggaagattgg gaagagcgta
     1621 agttaattga tttagttgtt agacttaata aaagtacaaa ctccagtaga cttccaaaag
     1681 ttgaatttga agatatcgtg gcaggagaag gtagactgaa taaagatgtg agccagaaat
     1741 ttgataatag aaaagggatt gaatttttac caaatgatat tctatatgga aagttacgtc
     1801 catatttaaa aaattggctc aaggcaactt ttacaggcgt agcgattggg gatttctggg
     1861 tttttagagc taagaagagc gactcggact ttatttattc tcttattcaa gctgatagat
     1921 atcaaaaggc tgcaaatgat acatctggaa caaaaatgcc tcgttcagac tggaaaaaag
     1981 tttcagatac agtattctat attcccaatg atttaaaaga acaacaaaaa atcggcatat
     2041 tcttcaaaaa actcgacgac actatcgctc ttcatcaacg taagttagat ttgctgaagg
     2101 aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa cggagcaaaa gtgccagaaa
     2161 tccggtttcc tggttttacg gaagattggg aagagcgtaa gttgggagaa ttaggtaaaa
     2221 ctcaatcggg aattggattt cctgatgccg agcaaggtgg ttctgaaggt actccatttt
     2281 ttaaagtttc cgacatgaat aacattggta atgagtatga aatgagaaat gcaaatcatt
     2341 atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc aataaaagat gtgccagcga
     2401 ttatatttgc taaagttggc gcagcaataa tgcttaatag aaaacgtttg gtcacaagcc
     2461 ctttcttaat agataacaat accatggctt atttatttaa taatacttgg gatatctatt
     2521 ttggtaaaat attgtttgaa actataaatt tgccaagata ctcccaagta ggggcattgc
     2581 cttcttataa cagttctgat attgagaata tcagtgttaa ggttcctgta aaggatgaac
     2641 aacaaaaaat cggtactttc ttcaaacagc tagatgatac gatcgctctt catcaacgta
     2701 agttagattt actaaaagaa acgaaaaaaa gcttcttaca aaagatgttt gtctaggagg
     2761 gaagaagaat gaaaattagt caccgtgagg aagcagaagt cgaagaacag ttgattcgtg
     2821 tacttggaga agggcataat cagtggacgt atcgtccgga tctaaaatca gaagaagacc
     2881 tttgggtcaa tttacgccaa aaaattatct caaataatca agcagaactg aatgattttc
     2941 ctttgaccga taaagaattt gaaacgatca aaacggaatt actgctgcga acgaaaaccc
     3001 catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat ggctcggatc acgattgaac
     3061 gggaagattc gcaattgggt tctgtctcac ttatccttta ttccaatcaa gacattggtg
     3121 gaggaatctc tacttatgaa gtggtccatc aaattgcaaa acgtggaagt aacatagaag
     3181 cacgggatcg acgttttgac gtcaccttat taatcaatgg attaccgatt gtccaaatcg
     3241 agctgaaaca agtaactgca aaagatggtg tttatcaggc atttaaccaa atcaaaaaat
     3301 atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg tttgtggttt
     3361 ccaatgaaca aaccactcgg tattttgcaa atgccttacc gaaagacttg caccctaagt
     3421 ttttatttag ttggcgaacg aaagacaatg aaaaagtaga aaacttatat gagttttgta
     3481 aacaagtgtt aaatatcccg gatgctcatc gtttgattgc ggattataca atcgtcagtg
     3541 aagatcaaga taataaaacc ctcatggtgt tacatcctta tcaagtacat gcgattcaag
     3601 ccttgtttat tgccgcaaat aaacatcaat cagggtatgt gtggcatgcg actggttcag
     3661 gaaagacgtt gactagtttt gtatctacta agcttttagc acgaaaatcg gggatcgatc
     3721 gaacgatcat gttagtggac cgcaaagatt tagataatca aaccaccaca gagtttacaa
     3781 aatttgcttc ggaatttaat actgggattt cttccgggaa cgccaaagcg aatagcttga
     3841 tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct gttggcagat gccaatgcaa
     3901 atgttgtaat cattacaacc cgtcaaaaac tagatgcggc gttgaaatat gcaaagaaac
     3961 aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat gggccaacat atcgtgtttg
     4021 tagtagatga gtgtcatcga gcgttaagtg ctgaaaatat ggaagaaatc aaaaaaatgt
     4081 ttccgaaatc cacttggttt ggctttacag gtacaccgat ttttcctgag aatcggaaac
     4141 aagccaaagg gcaattggca cgtacaaccc atgatcagta tggagaggtc ttgcatacct
     4201 ataccattaa gaatgcgcta gaagatggct ctgtcttagg atttcaagta gagcatgaaa
     4261 acaccgtgga gccgacttca ctagagaata aaatctatcg gaaattaaaa gaagtagaaa
     4321 catatgcaga gtattcatca gaacagatca atcgtatgat tgatcaaatg gaacctgtca
     4381 aaaaagaaag ttatttggac ccagctgttt ttgaagcaga tgaacatatc caaaaagtca
     4441 ttcataaaat gttccgtcca gataatgcct atacaaaatt tgattttcga aatggcaggc
     4501 caacgaaatc tgcaatctta accacgagtt ccattgacat ggccaaaaag tattatcggg
     4561 ccatcaaaga gatgacgaaa gagcctgatt ggttaacaaa agagtttccc aatcagctca
     4621 ttcgtgaagg acgaacgatg gaggatcctg attttccacg aatcgctatt acctattcat
     4681 tagaagaaaa tagccgagat tcaagtgccc aacaagaaga aatgcaaaaa attattgagg
     4741 aatataatca gtattatgat acttcctggt cattagcaga cattgagcgt tataacggag
     4801 acatcaataa tcgtttggca cgaaaaagag cggaattcaa acaatttggg aaacaaatcg
     4861 atttagcgat tgtagtggat cgtctattaa caggttttga cgcccctacg attcaaaccc
     4921 tatttgtgga tcgtaatcta gagtatgcag gattgatcca agctttttca cgtacgaatc
     4981 gtacgtatcc tgataagaca aaaggattga ttgtgacgtt tcgtaagcca gcgacaatgg
     5041 agaagaatgt cgaggatgcg acaaaactgt attcaaaagc aaaagaagaa tcaggtttga
     5101 tttatccaag ttaccaagaa tccaaaaaaa gattcaaaca agcatatggt aagttaaaag
     5161 aggtttcatc catggaatcc attgatgaac atacgccact tgaaatccgt atcgagtacg
     5221 tcaaagcatt tcaagaattg aatcgt
//
LOCUS       DAXWLB010000170         5152 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXWLB010000170 DAXWLB010000000
VERSION     DAXWLB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.173"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            44..1333
                     /locus_tag="ACGE4H_002474"
     CDS             44..1333
                     /locus_tag="ACGE4H_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFF8365854.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1459..2538)
                     /locus_tag="ACGE4H_002475"
     CDS             complement(1459..2538)
                     /locus_tag="ACGE4H_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFF8365855.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2647..3087)
                     /locus_tag="ACGE4H_002476"
     CDS             complement(2647..3087)
                     /locus_tag="ACGE4H_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFF8365856.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3144..3554)
                     /locus_tag="ACGE4H_002477"
     CDS             complement(3144..3554)
                     /locus_tag="ACGE4H_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFF8365857.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3735..4169)
                     /locus_tag="ACGE4H_002478"
     CDS             complement(3735..4169)
                     /locus_tag="ACGE4H_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFF8365858.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4269..5117)
                     /locus_tag="ACGE4H_002479"
     CDS             complement(4269..5117)
                     /locus_tag="ACGE4H_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFF8365859.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1893 a          996 c          871 g         1392 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 agacccagaa gaaaaaccgg atgtgactcc agaaccagat acggattctg gaaaccaaac
      781 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
      841 agccccagaa gaaaaaccag atgtaactcc agaaccagat acggattctg gaaaccaaac
      901 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aactagaggt
      961 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
     1021 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
     1081 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
     1141 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctagtcc
     1201 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     1261 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     1321 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     1381 ccagaaaacg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     1441 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     1501 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     1561 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     1621 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     1681 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     1741 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     1801 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     1861 cgtgtttaaa agagcgataa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     1921 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     1981 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     2041 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     2101 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2161 aacaggatct gcatcttttg gctgatgctc gtaaaaatca ataacggact ggatcatatc
     2221 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     2281 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     2341 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     2401 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     2461 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     2521 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagaa agagcaaaaa
     2581 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
     2641 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
     2701 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
     2761 accttctgct tgtttttgca acgcagcacc ttgaggagtc agcgtaacat ttacgattct
     2821 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
     2881 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
     2941 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
     3001 gaggagggaa gtgtagtgtc taatgatttc cttagaggcg gcatataaag gaaaacatac
     3061 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
     3121 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
     3181 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
     3241 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
     3301 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
     3361 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
     3421 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
     3481 gtacgatcgg gattatgaac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
     3541 tacatttttt tcataaaatt accaccttta attatatttt atacaattga attttacaca
     3601 atataattgt ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga
     3661 aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa atatcagaga caaaaactaa
     3721 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaacttact
     3781 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
     3841 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
     3901 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
     3961 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
     4021 gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
     4081 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
     4141 acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
     4201 tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata gcggatttac
     4261 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
     4321 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
     4381 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
     4441 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
     4501 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
     4561 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
     4621 aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
     4681 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
     4741 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
     4801 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaaac
     4861 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
     4921 aacataacga tattcaacac taaaataact atagctcggt caaatcctaa aacagcttcc
     4981 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
     5041 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
     5101 tgtctcgttt ctttcattta tccacttcct taattcatta ataatactct ta
//
LOCUS       DAXWLB010000171         5076 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXWLB010000171 DAXWLB010000000
VERSION     DAXWLB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.174"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            202..1476
                     /locus_tag="ACGE4H_002480"
     CDS             202..1476
                     /locus_tag="ACGE4H_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365860.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1530..2957
                     /locus_tag="ACGE4H_002481"
     CDS             1530..2957
                     /locus_tag="ACGE4H_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFF8365861.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2970..3842
                     /locus_tag="ACGE4H_002482"
     CDS             2970..3842
                     /locus_tag="ACGE4H_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFF8365862.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3937..4932
                     /locus_tag="ACGE4H_002483"
     CDS             3937..4932
                     /locus_tag="ACGE4H_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8365863.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1751 a          699 c          991 g         1635 t
ORIGIN      
        1 aaaaatagtg ccattaaaaa agaaaagcga cacaccaata aaagtcatat cctgtgacat
       61 tatatggcat tatccaaata gaataaccgg ttgacatgta agcggatttc ttttataatt
      121 ttaggagtac aagtcctttt ctaaaaatgt taaccggtta tcatgaaagg ggatacaaat
      181 atatcgttgg aggataaaaa tatgtcaaaa gttaacgtat ttaaaaatca atcttattta
      241 caaagttcag ctacattatt actatttttt gcttcttggg gtgtttggtg gtcatttttt
      301 caactttggc taacatctga atcaaatggt ttagggttat ctggcagtgc tgtaggaaca
      361 gtattctcgg caaattcgtt agttacctta attttgatgt ttatttatgg aacattacaa
      421 gataaattgt atattaaacg aaatttatta atttttgctt ctgtattagc gacacttgtt
      481 ggaccatttt ttatatggat atatgggcca ttgctagata acaattttaa tttaggcatt
      541 attatgggag cgctattttt gtcagctgga tatttagctt ctgtaggagt ttttgaagct
      601 gtgtcagaaa ggtttagtcg tttatttggc tttgaatatg gacaagcaag ggcgtgggga
      661 tcatttggtt atgccttggt agcgcttttg gcaggatttt tatttgtaaa aaatcctcat
      721 ttaaactttt gggcgggatc tttctttggt tctttactat tgttaaattt attattttgg
      781 aaccctaaag ttgaacggga agcaaatcaa aattttaatc aagaacaagc tgaatcaaat
      841 agtattcctt ctttaaaaga aatgtttgat ctaatgaaac tgcctcaatt atggacgata
      901 atcatcttta ttgtttttac atggacattt tatacggtat tcgatcaaca aatgtttccg
      961 ggattttata ctggtttgtt ttcaacatca gctaatggtg aaaaaatata tgggacattg
     1021 aatgctattc aagtattttg tgaagcgtta atgatgggaa ttgttccaat cattatgaga
     1081 aaattagggg ttcgaaatac tttgttatta ggtgtaacca ttatgtgtgt acgaattgga
     1141 ttgtgcgggt ttgcctcgac accattatct gtttcatgca taaaaatgtt gcatgcttta
     1201 gaagtaccat tatttacatt accaatgttt cgctatttta cacttcattt tgatacaaag
     1261 ctatcagcaa ccctctatat gataggattt cagatagctg ctcaaattgg gcaagtgatt
     1321 ttatcaacac cattgggaat attaagagac aacgttggct atcaaccaac atttaaaatt
     1381 atttctctta ttgtattact agcaggcata tatgcattct ttattcttaa acaagatgat
     1441 agagatgttc aaggggatcc atttattcga ggataatgga aaccaacaga ataaaaaaca
     1501 attttatcta gaaagtgagc gaatatatta tgacaaaaag agctatagaa ttaacaaata
     1561 cacgttatcg tttgggatat catgtgtctg caccatcagg atggattaat gatccaaatg
     1621 gattttgcta ttttgatggg tattatcatg tgttttatca acatcatcca tatagtgctg
     1681 aatggggacc aatgcactgg gcacatgcta gaagtaagga tttagttcat tgggaatctt
     1741 taccacttgc tttgactcct ggagatcaag aagatgaagg tggttgtttt tcaggaagcg
     1801 ctattgaaaa aaatggtgtg ttgtatctgt tctatacagg acatcactat tatggagaca
     1861 atgatccaga tcatttttgg caaaatcaaa acatggcgta tagtacggat ggcattcact
     1921 ttactaaata tgaaaaaaat cctgtaatag caaaagcacc agaagataac acacatcatt
     1981 tcagagatcc aaaagtatgg aaacacgatg aaaaatatta catggttttg ggtagtcaag
     2041 aaaaagatgg attaggtcgt gctattgttt atcagtcgtt agatttatta aattgggatt
     2101 acattggacc aatttctaaa gcgaaggaag tacttacaga aggatttatg tgggaatgcc
     2161 cagacttttt tgaattaaat ggaaaatcta ttcttttatt atcccctcag ggaattgaag
     2221 caaatggaaa agattattta aatctatatg aaacaggcta ctttatcggt gattatgatt
     2281 atgagacagc aaaattcatt agaggaaatt tttatgaatt agataaggga cacgatttct
     2341 atgcaacaca aacaacttta tctccagatg gtagacgtat tgttatggcc tggatggata
     2401 tgtgggaaag ccctatgcct gaaaaagtag acggatgggc aggagcatta acgcttcctc
     2461 gagaattaga gttaattgga gatcgtttgt atatgaaacc tgtgagagaa ttagaacaac
     2521 ttcgagttgg cagcggcgtt gaaacgacag ttaatttggc aggagaaata ataatcgctc
     2581 aagatgcttc attcaaagaa gctctaattg atgtactatt gactaaatca aaaaatcaag
     2641 aagtcaaatt ttcattaaaa acaaaagatg aagagttaat caatattttt tattctaagg
     2701 ataaaaatga atgtacctta actcgtaacg gcaaaaatga tccaaggtat ggaaccattc
     2761 agatgtgtga tagattatca cttcgagtgt ttatcgataa aagttcggca gagattttta
     2821 ttaatgatgg cgagcttgtt tttacagagc gttattatac agaaaatcaa ccaagtatgc
     2881 atttgagtgt tagtgaagaa gcttttgtct cgagtacaat ttatcatcta aaaaaagatg
     2941 caatttctta cgagtaagga ggttttatga tgttaattgg tagcatagaa gcaggtggga
     3001 ctaaatttgt atgtgccgtg ggagacgagg attaccgagt acaagaccaa ataatttgtc
     3061 caacgaccac ccccagggaa acattgaaaa aaactgttga ctattttaaa cagtttgata
     3121 taaatgcatt aggtattgca tcatttggtc caatagaaat tcgaaaaaat tcaccaaaat
     3181 caggatatat tacgtctaca ccaaaggtgg gatggcgaaa tgttaatgtg ttaggttatt
     3241 tacaagatga gctgaatgtt ccaatgtctt ggacaacgga tgtgaacgga tcagcttatg
     3301 gagagtacat catgtccact ttatcgaatg aaaaaataaa ttcattagtt tattacaccg
     3361 ttggtactgg cgttggtggt ggagctgttg ttgacggaaa actattagga tcgtttggtc
     3421 attcagaaat gggccatacg tttgtaaaac gccatcctga tgatattgat tttaaaggaa
     3481 tttgtcctta tcatggtgat tgtttagaag gcttagttgc gggaccaacg tttgaagcac
     3541 gttttggaaa aaagggtgaa gacgtttctt taacggatcc tatttgggat gtaatggcct
     3601 attatctagc acaagctgtg attcaaacaa cattgattat acgaccggat aaaatagtat
     3661 ttggtggaag cgtagtaaat gaaaaattgt tagaaaaaat tcgtgttaat gttaataaat
     3721 tattggatga ttacgtggaa ataccaccgt taagtaaata tattacgcgt cctttaatta
     3781 aaaataatgg atcggcaaca ctaggaaact ttgctttggg cttgaatcaa ttacaagaat
     3841 aaaacataga aaaaatatcg attatcaggt atagtagttc tatagagttc tttttttcga
     3901 tagttaatta ttgcattatt taggagagac aagagaatgc gcccaaaatt agaagatgtt
     3961 gccaaaagag caaatgtatc aaaaacaact gtttcacgtg tattaaacaa tcgtggctat
     4021 ttaagtcaaa aaacgattga taatgtttat aaggctatag aagaactcaa ttatcaaccg
     4081 aacgttgtag cgcgtcaatt atttcaaaag aaaactaaca ttgtcgggtt actatttcct
     4141 acagtagcca atcctttttt tagtgaactc gttgaggcat tggaaaaaaa gttatatgaa
     4201 attggttata aggtattaat aggcaattcg atgaataata aagaaaagga aacaaattat
     4261 ttaaatcagt tattgtctga tcaagtagat ggtttaattg ttggtacaca taaccaagga
     4321 attcaggaat ataaatatca gaatttaccg attgttgcaa ttgatcgagt gatgaatgaa
     4381 gatattccag ttgttgaatc cgataactac aatggtggaa aacttgcaac caagttatta
     4441 attgcacaag gtgcaaaaaa tattattcat acaaacggtc caattgactt acagactccg
     4501 gctaatcgtc ggagactagc ctatgaagat actatgaaag catatcagtt aattccacga
     4561 actgtgacat tagatttcaa tatttcttat gtaaagaaaa aacagatttt tttccaaatg
     4621 tttgaggatt atcctaaaat agatggtatt tttgcttcaa atgacatcga tgcggcccta
     4681 atattacaag tagcaaaaga aaaggggtta aatgtaccag ctgatttatt agtagtcgga
     4741 tatgatggta ctttaatgac tcggagtata ttaccagatc tgacaacagt aatacaacca
     4801 attaatgata tagcagacac agctgttgct attttgatga aacgaataaa taaagaagaa
     4861 acaaaaaagg aatatatttt acctgtcaca ttatggatgg ggaagacatc agtaaagaaa
     4921 aagaacaatt aaatttataa agactatcaa tgataatttc attattaatt cgtgatataa
     4981 aaaattgtta aatctcttat aatagactat gatacaaaat catgctttta atctgtgtag
     5041 atgttgccaa atatttgaaa gacatagaac ttgtca
//
LOCUS       DAXWLB010000172         5006 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXWLB010000172 DAXWLB010000000
VERSION     DAXWLB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.175"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..376
                     /locus_tag="ACGE4H_002484"
     CDS             <1..376
                     /locus_tag="ACGE4H_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HFF8365864.1"
                     /translation="INSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPKDSGSE
                     VADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAVVQTSS
                     EDTYMINFDPYPEQIERFDGGQ"
     gene            398..1534
                     /locus_tag="ACGE4H_002485"
     CDS             398..1534
                     /locus_tag="ACGE4H_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFF8365865.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            1549..2199
                     /locus_tag="ACGE4H_002486"
     CDS             1549..2199
                     /locus_tag="ACGE4H_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFF8365866.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATSSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDST
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            2213..3109
                     /locus_tag="ACGE4H_002487"
     CDS             2213..3109
                     /locus_tag="ACGE4H_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365867.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMMIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            3145..3375
                     /locus_tag="ACGE4H_002488"
     CDS             3145..3375
                     /locus_tag="ACGE4H_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365868.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            3726..4022
                     /locus_tag="ACGE4H_002489"
     CDS             3726..4022
                     /locus_tag="ACGE4H_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365869.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVKKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            4019..4486
                     /locus_tag="ACGE4H_002490"
     CDS             4019..4486
                     /locus_tag="ACGE4H_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365870.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKANMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSIHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEKESSRLGDGTMFSSPSLRFTTD"
     gene            4568..>5006
                     /gene="mobL"
                     /locus_tag="ACGE4H_002491"
     CDS             4568..>5006
                     /gene="mobL"
                     /locus_tag="ACGE4H_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFF8365871.1"
                     /translation="MTEHIFNRKSASIILKAKFETGKNKKKNHMVELQKFVDYISRQE
                     AIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTRKKAIL
                     ENIDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQD"
BASE COUNT         2060 a          601 c          921 g         1424 t
ORIGIN      
        1 tatcaattca aataacaagt atgcttgtga atatgtacta agttttgagc gagaaatgcg
       61 aaaaatgcgt gcaggtgttt attttgcaac tcaaacgcct gaagaaatta tccctaaaga
      121 ttctggatca gaagtagcag atgtgattag aaacattttt aatctatgta catttaaatg
      181 cttctttaat ttagattctg cattgttgga agacataaaa aaagttttag gaaatacaat
      241 tactgataca gaaattatgc ttttaccaga attagaagta ggacaagctg tagttcaaac
      301 ttccagtgaa gacacctata tgattaattt tgatccatat ccagagcaaa ttgaacgatt
      361 cgatggtggt caataataga aatcggaggt gaatttaatg gagcagaaca aaaataataa
      421 tgttgcttca acactcgctc gtcaagggat gaagcaagta tcaaagaaaa taggtcttgc
      481 tttaaaaata aaaatagggg tagttatagg actgataaca ttggttggcc ttttagttat
      541 aggttcttta gctacattac aaggaacaca aactgcaact aatacaggag gtgcagaagt
      601 cattggtggc aacaaaccgt tgtcacctga agttcaaaaa tggcaaaaaa cggtagaaaa
      661 atatgctgaa aaatacggaa ttaaagaatt agtaccttat gctttagcaa ttatgcaatg
      721 tgaatccgga ggtaaattgc cggatttaat gcaaagtagc gaatcagccg gattgcctgt
      781 aaacacactt caatatgaag aaagtatcga tcaaggactt aaacatttga gtaatgtgta
      841 taacattgct aaaagttatg gcatggaaag taactatgat ggaatagttc aagcttacaa
      901 ttacggcata gcttacatgc actacttagg aaaaaacaaa aaaacacata ctatcgaaac
      961 ggctgaagcc tactctaaaa atgttgttgc tccaagttta ggaaatacta caggacagac
     1021 ttattcatat gtaaatgaag tatcaattaa atatggaaaa acatatttgt atttaaatgg
     1081 tggtaatttt tactatgcag agttagttaa acagtatatc ggtgggggta gtggtaatcc
     1141 ggctaatcct tcagaatttt ggggaaaaat gaaaacagaa atggaaaaat atcaaggttg
     1201 gccttatgtt tggggcggta aaaatccaac gacaggtttt gattgtagcg gattaacagc
     1261 ttatctttat caatttagcg ccggaattac aatagatagt tatacagtaa cgcagtatga
     1321 agcaactgaa gcagtatcaa ttaaagaagc aaaagctggt gatctgattt tctttagagg
     1381 aacatatgga gcgcctgatt tcatttctca cgtaggtatt tatgtcaatg aaacaactat
     1441 gtatgacagt aattcaagcg gaattggtta tcacaattgg caagattcat attggcaatc
     1501 acatagtcca gagattcgta gagtcaaaaa ataggaggaa gaaaatagat gattaagaga
     1561 aaaaatgata ataaatggaa atatatttgt ggtgttttaa ttattatttt agttttaggc
     1621 ggattgttat ctcaacttca tacaagtaac attcaagcaa aaaataaaaa attggatcaa
     1681 gaagttacac aactagaaga acaaaatagc aagttaagta aaaatattag tgatctaaat
     1741 gcgaatgtag aagcgaactt aactaatgat gatggtagtg aattaaataa acaaattgct
     1801 gaagttgcta agaaatttgt tgagctatat ccggtatatg atattgaaaa agtagaagaa
     1861 aagaaagctg atttagagaa aatagctact tcttctgtag ctgattctat cgtgccggat
     1921 gacatgatta caacgagtaa aaagacttta gaaagtaaat ctactactca aaaaggggaa
     1981 gcatattcaa gcgatccaac atttaaaagt aaatataaag attcaacatt atatacgaat
     2041 tatgttaacg ccaatactat taattatttt gctgaagtca attataaaac agaatcttct
     2101 agtggagata cagaaaatac agtctatatg ctttttgaag taaccaataa agacggccaa
     2161 gtgttggtag ataattatga aatcaagtac ttaaaatagt gaggtgaagt atatgaataa
     2221 gaaatattcc attcataata agaaggaacc aaataaaaaa aataaggcac aaattgacaa
     2281 agtatataaa aaaacgaaag gagtaaaatt atatttaggt gctatacttg gatttttagc
     2341 aataggaatg atgatcgttt tttctcctta tgtgatgggg aaaagttatg attttgaaac
     2401 tgtaaaaatg agttcagccc aaaatgtaac gtcttctttg aatatgtatg ttgatgatat
     2461 ggaaataaat agggaagccg gactgtttaa attagtgttg aggtatcaag atgatacagg
     2521 tactaaatca ctaagtaata ttaaaagtga ctataaatta aattttataa cgaacaaaaa
     2581 taatgaagat gttaagacaa aagttgtaaa attgtcagat gaatacacgg taatttatta
     2641 tcaaaatcta cctaaagatt ttggtgtaat ttctgtgaca gttaacccta gatatattta
     2701 tcctgaatta gaaccttcaa atgacttaaa agaaaaggaa ataaaattct atgcagtaga
     2761 caaagatatt aaatcaaatg ctaaattgga agttgaaagt acttctgatt tgcaaaaaga
     2821 taattttaaa tatcagatta aagctataga aaattctata aaagatgaga aaaaatcaat
     2881 tgaaaaaatg aatttagcaa ataaagtagt gcaaaaagat attgataaag ctaatcaaaa
     2941 cttagaattt caaaccctag acgaacaaac agatacaaag aatgaaatca gttctatgca
     3001 gacgactatc gaaaataata aatcttcaat agaggaaagt gaaaataaaa tcaaacaatt
     3061 tgaggaaaaa ataaagcaac tagaaaagtc tgcaagccga tttgagtgat cgtgtttaaa
     3121 ttattttaag ggagtttaga gagaatgtgt aattctattg tcactattca agagaaagct
     3181 gaagaagaat taatgagaat tgaagcggaa tataaagaaa agagaaaagc aattaagcgc
     3241 aaaaaaaatg cagagcttaa aaaatataaa gaggaacaag cgttaaaatt aggaatgatg
     3301 ttgtgtaaat attatgattg ttatgatata aacgaagtgt taaaaaaagc gaatttaaac
     3361 caattttgtg aatagaaaag agagtggcaa ggtaaaactt gtcactcttt ttttgtgcgt
     3421 aaaagcttta gttactatga ccaaaactac ggacgaaact atgaccaaaa gtaggtacta
     3481 tattatgacc gaaaataggt cgaaaatgga tttatttgtg atagcaaaaa atatgaagtg
     3541 attttgaaag aaaaccagtt ggcacagcca acttagccac cttcctttag gaaggtgcta
     3601 tcagttttcc cttatgctct ttgcattaat tctttggttt cgtctttaaa tctttaattc
     3661 tcctttagac tttgatataa taatatatac cagatagata aaaactattt gaatgaggtg
     3721 tatatatgga cgtttcaatt agagggattg atccgcaata tgtaaaagag attgataaaa
     3781 aagttaaaaa aattcaagaa cgtaccggaa aaaagtttag taggaatgac tattttttag
     3841 caatcattcg ccaagatagt gagattgatt tgattgatta taaaaagtca gaatttgatt
     3901 tagtagttga aaaactatta ttgtcaaatg agcaaatggt agaaagtatt agcgagttaa
     3961 cttcagttta tgaacgctta ttcaattttt taattagcga aaggagtgat ctaaataaat
     4021 gaactaccct tatttcaaag tatcagcaac agaagatgta aaagaaaaat tcaataagtg
     4081 ctttgaagta aacaagtcag tatttcaaac aaaagctaat atgttccgat atgttgtttc
     4141 aaatttacca atgttaacaa aagccaatca aaatttccgt gatccagaaa aagaagagtt
     4201 agctaaaaac ttagaagaag caaaattaaa aattagcgaa cttgaaacga taatagaatt
     4261 gcaaaaacag caacttgaaa actttaataa aaaagaagac agtatacaca aagattatag
     4321 tgaacaatta aatagtatag aagagtctgt ctataaacta ttaattaata gtggattagc
     4381 tagtaaagaa ttagaagaag agcgtgctga accagaaaaa gaaagtagtc gtttaggaga
     4441 tggaacaatg tttagttcac cgtcattaag atttacaacg gattgaacac taaaaaattc
     4501 taatgcaact tagcattaag tacaaaaccg cctgtagaaa aaaattttga aaatggtggg
     4561 tgatgatatg actgaacata tctttaacag aaaaagtgcg agcattattc ttaaagcaaa
     4621 atttgaaact ggaaaaaaca aaaagaagaa tcacatggta gaacttcaaa aatttgtaga
     4681 ttacatttcc agacaagagg ctattaggca agataaatta gatcatgatt attccgaaga
     4741 tgaactaaat gaattagcta gaatagaaaa agctttggaa aaaatagaac aggaaacaga
     4801 tcaaccagtg attaaagatt tgcgtgaaat ggataaatat attgattata tgacacgtaa
     4861 aaaagctatt ttagaaaata tagataatga aatagtaaac ggagctttta gtaatacaaa
     4921 aagatatatc actaaaaaag acatttcaaa aataaaagaa tcggttatag aagcaaaaaa
     4981 taatggatcg gtgatgttcc aagatg
//
LOCUS       DAXWLB010000173         5001 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXWLB010000173 DAXWLB010000000
VERSION     DAXWLB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.176"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(179..763)
                     /locus_tag="ACGE4H_002492"
     CDS             complement(179..763)
                     /locus_tag="ACGE4H_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8365872.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1094..1327)
                     /locus_tag="ACGE4H_002493"
     CDS             complement(1094..1327)
                     /locus_tag="ACGE4H_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFF8365873.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1342..2292)
                     /locus_tag="ACGE4H_002494"
     CDS             complement(1342..2292)
                     /locus_tag="ACGE4H_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFF8365874.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2289..3131)
                     /locus_tag="ACGE4H_002495"
     CDS             complement(2289..3131)
                     /locus_tag="ACGE4H_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFF8365875.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3133..3852)
                     /locus_tag="ACGE4H_002496"
     CDS             complement(3133..3852)
                     /locus_tag="ACGE4H_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFF8365876.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            4400..4702
                     /locus_tag="ACGE4H_002497"
     CDS             4400..4702
                     /locus_tag="ACGE4H_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365877.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            4774..>5001
                     /locus_tag="ACGE4H_002498"
     CDS             4774..>5001
                     /locus_tag="ACGE4H_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8365878.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFY"
BASE COUNT         1724 a          871 c          654 g         1752 t
ORIGIN      
        1 gaaaaaaaat atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc
       61 caaaaaataa gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac
      121 agtgcgatct tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt
      181 aactcagtaa ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca
      241 tagtcttttc ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt
      301 aaatgtagaa gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag
      361 tctttaactt atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa
      421 caatatcttg atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat
      481 tctcaaaaat ataattaata aatggatcta attcattgaa gttctctctc caacttttct
      541 ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa
      601 taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat
      661 gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt
      721 gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta
      781 taatataatc tctggtttct atgggtactt cctcctaata agatgaatta actttattat
      841 cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc
      901 taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag
      961 actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat
     1021 ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta
     1081 aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat
     1141 cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac
     1201 ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt
     1261 ttctttttga taattcctga attgctagta aataaattag tatagtagtc gttttaattt
     1321 gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca
     1381 tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa
     1441 aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc
     1501 cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg
     1561 ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg
     1621 cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt
     1681 atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc
     1741 aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa
     1801 ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg
     1861 atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc
     1921 tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc
     1981 atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt
     2041 ttgaacatct tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact
     2101 gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt
     2161 aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga
     2221 acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca
     2281 ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga
     2341 aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta
     2401 aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata
     2461 aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac
     2521 attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat
     2581 agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat
     2641 ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa
     2701 accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat
     2761 aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct
     2821 aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt
     2881 ataaaagtaa taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta
     2941 atacctaaaa tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc
     3001 ataagagaca ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata
     3061 actacagatg taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa
     3121 ttttgaagca tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa
     3181 attttcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
     3241 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
     3301 ttttccctga tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc
     3361 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
     3421 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
     3481 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
     3541 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
     3601 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
     3661 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
     3721 aagcaatgtg gattttccag caccatttgg accaataata cctgttaaag caccttcttg
     3781 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
     3841 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
     3901 aaaaattata ttttagtcaa ccctaaattt tataatttag atttttttct gttgctgaag
     3961 attagaatga caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa
     4021 aaaaggagcg ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa
     4081 attatacttg aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa
     4141 attattcaag actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac
     4201 ttttttcgta gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa
     4261 actatttttg atcattgaca attaaataca atttttgaat aacgatccct gctattcgag
     4321 ctagaaactc aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt
     4381 tctgggaaaa caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc
     4441 cgtaaactgg ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat
     4501 aactactatt ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac
     4561 ctgtgcattt ctaggaatcg ataataatac tctagataag tggattcgta tatataattt
     4621 cccagtaata agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat
     4681 gaatacattt aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat
     4741 atactgcctt acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa
     4801 tataaaacta atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa
     4861 aagtatcaaa aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag
     4921 aagaaaatag aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa
     4981 ataacgttta aaaaatttta c
//
LOCUS       DAXWLB010000174         4980 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXWLB010000174 DAXWLB010000000
VERSION     DAXWLB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.177"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..194
                     /locus_tag="ACGE4H_002499"
     CDS             <1..194
                     /locus_tag="ACGE4H_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365879.1"
                     /translation="KFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSDKKIAYPL
                     LLLLFSFYQVQKLFCFVIC"
     gene            complement(171..1781)
                     /locus_tag="ACGE4H_002500"
     CDS             complement(171..1781)
                     /locus_tag="ACGE4H_002500"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFF8365880.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2003..2248)
                     /locus_tag="ACGE4H_002501"
     CDS             complement(2003..2248)
                     /locus_tag="ACGE4H_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365881.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2974..4314)
                     /gene="glnA"
                     /locus_tag="ACGE4H_002502"
     CDS             complement(2974..4314)
                     /gene="glnA"
                     /locus_tag="ACGE4H_002502"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFF8365882.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4342..4734)
                     /locus_tag="ACGE4H_002503"
     CDS             complement(4342..4734)
                     /locus_tag="ACGE4H_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFF8365883.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1478 a         1064 c          812 g         1626 t
ORIGIN      
        1 agaagttttt tgcaaaaaat aaaatatcca atagcgcttt aatcgaaaaa agtcttttgc
       61 tttctatctt ttttcctaca aataataaca aatcagaaaa agagaaaagt gacaagaaaa
      121 ttgcttatcc ccttctcttg ctacttttct ctttctatca agttcaaaaa ttattttgtt
      181 tcgtgatctg ctaatgatgc tccgacaaat gctttgatta atttttgcgg gcgatttgga
      241 cgagaaatca attctgggtg gaattggcat cccacaaaga attttttctc tggcagctct
      301 acgatttcta ctaaacggtt atctggtgag acacctgaaa agactagccc ttgttcttca
      361 aacaattgac gatatttgtt attgaactca tagcggtgac ggtgacgttc ttgcacaaca
      421 tcggctccat catatgcttc agccgtcttc gttccttttt tgattttaca tgggtataat
      481 ccaaggcgta aagttccacc caaattctcg atgttttcct gatcagccat taaatcaata
      541 atattgtttg ctgtatctgg attcgtttca gctgatgacg catcttctaa tccaacgacg
      601 ttacgtgcaa attcgacaca agccatctgc ataccaagac aaatacctaa aaacggaacg
      661 tcattttcgc gagcataacg aatcgcttcg attttccctt ctactccgcg atcaccaaat
      721 ccaccaggaa ccaagattcc atctgcgtct ttcaaacgtt cttctacatt ttctctcgtt
      781 aattcttgtg ctttgatcca atcgatctcg atgtctgaat tataggcgaa accagaatgt
      841 ttcaatgctt caactactga gatatatgca tctggtaact cgacatattt accgacaagc
      901 gcaatccgta cttttttctt caagttcaag acacgttctt ctaattcttt ccattctgtc
      961 atgtctgctt ctggtgtatc tagttttaaa tggtcgcaaa caatttgatc cattccttgt
     1021 gcttgtaaaa ctaatgggat ggagtaaagt gtatctacat ctggcgactc gataactgct
     1081 tctggttcga catcacaaaa ttgtgccaat ttgtttttca cgctttgaga aacaggttgc
     1141 tctgtacgta ccaccaaaat attcggttgg atacctaaac cacgtagctc ttttacactg
     1201 tgctgcgttg gtttagtttt catttctcca gcagctttca agtaaggaat caatgttgta
     1261 tggatataca taacattatc tgtaccgaca tctgctttca tttgacgaag tgcttcaagg
     1321 aatggcaaag attctatatc ccctactgtt ccccctactt ctgtaatgat cacatctgaa
     1381 tcagtcattt tagctgctcg catgattttt tctttgatct catttgtaat atgaggaata
     1441 acttgaacag ttgctcccaa gtattcgccc ttacgttctt tacgcaaaac ttcagaatag
     1501 atttttcctg tcgtcacgtt ggagtatttg ttcaaattga tgtcaataaa acgttcgtag
     1561 tggcccagat ccaaatctgt ttcagcacca tcatctgtta cgaatacttc tccgtgctga
     1621 tatgggctca tcgtccccgg atccacgttg atgtatggat caaatttttg gatagttact
     1681 tttaagccgc ggtttttcaa taagcgtcct aaagaagctg caacaatccc ttttccgatt
     1741 gatgaaacga caccgccggt cacaaaaata tattttgtca taagttcaaa aactcctttt
     1801 taatttatgt gcaggaaaag tctcagacaa taaacaaagc tccctattct tcaataagaa
     1861 tagggagccg aaatttctgt aaacttctga ccctctaaaa gggtgcccaa ggtgtataat
     1921 acaagctgac taagggaaag tcaagtactc aaatgaatac tatccgctta caattttttc
     1981 tttttcgata tcctttgaga aattatttct taagtccttt atgagtgcaa aaaaaattta
     2041 acccacataa taagcttgat ataaacatag ctaacgttaa acctaagcaa atcagaaaat
     2101 cgttactgaa aaattttaag tgaaataata gataaaaaaa taaaacaaac attatgatag
     2161 ataatatccc acctaacaaa gaactacttt ttcctacttg cagcaacagt ttatatagct
     2221 tatcgtagaa acaatctttt tctttcaccc ttatttacct cctttacgta aaaaacgtag
     2281 tccacagttc tcggattttt ggcatatctt ttctattaaa taatatcaaa aacaaaacaa
     2341 ttccactaat tttttcaaag atgacctttt tttcaacaaa agtgccttgt agggattcat
     2401 tgaaggtaac gaatcacata agtaaaaaat aaggagatgc gacaaaagtc gtgcctcctt
     2461 atttctaaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccatca
     2521 aacatgagaa gctgtttttg aaattatttc ttgtgattcg gagttaaatg gtaggctcac
     2581 aacctctgga taaacggtat tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     2641 aaatgtagaa agccattttc acaaaatttt tcttaatact cgaaactaaa cgtctttttc
     2701 aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt tcagccaatt
     2761 caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca gggctgcttt
     2821 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
     2881 cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt caaaacgctg
     2941 atttcacagc ctctaattca cggtattcct gttttaataa agttctagat attgttctct
     3001 ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg cttcaacaaa
     3061 atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac gcaattcttt
     3121 gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc tttcttcctc
     3181 atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat tacggatacc
     3241 gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag atggatcaac
     3301 tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta ctaatggcga
     3361 acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa cgagacgttt
     3421 ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt tcagcaaacc
     3481 acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat catagaaaac
     3541 atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat tgatcccaaa
     3601 taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg ttttgacaac
     3661 gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt taaagtcaat
     3721 ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc ctagactttc
     3781 taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg caaaatcaaa
     3841 ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta atttaaataa
     3901 gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc gcatatcttt
     3961 caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat ccggattata
     4021 gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga agatcatcca
     4081 agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa atccttcaat
     4141 cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc tcactggaac
     4201 ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa atcgtacgtt
     4261 ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta ccatggtatc
     4321 cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt gcgactgata
     4381 atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa tttgtcgaac
     4441 gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct gtgccatttc
     4501 gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt taatctcaag
     4561 caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc gttccggatg
     4621 aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag tcagcttcat
     4681 aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc ccatcagacg
     4741 tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat tgtcaactgc
     4801 atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa aaaataagaa
     4861 cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt attatactgt
     4921 actatccttt attagtgttt ttattgcgcc gtttgtataa ttaagcgaga caaataaaaa
//
LOCUS       DAXWLB010000175         4977 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXWLB010000175 DAXWLB010000000
VERSION     DAXWLB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.178"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..182)
                     /locus_tag="ACGE4H_002504"
     CDS             complement(<1..182)
                     /locus_tag="ACGE4H_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFF8365884.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACGE4H_002505"
     CDS             517..951
                     /locus_tag="ACGE4H_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFF8365885.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACGE4H_002506"
     CDS             974..1294
                     /locus_tag="ACGE4H_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365886.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACGE4H_002507"
     CDS             1435..1626
                     /locus_tag="ACGE4H_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365887.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACGE4H_002508"
     CDS             complement(1697..1954)
                     /locus_tag="ACGE4H_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFF8365888.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACGE4H_002509"
     CDS             2112..2375
                     /locus_tag="ACGE4H_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365889.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACGE4H_002510"
     CDS             2399..2587
                     /locus_tag="ACGE4H_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365890.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACGE4H_002511"
     CDS             complement(2670..2855)
                     /locus_tag="ACGE4H_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365891.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACGE4H_002512"
     CDS             3070..3441
                     /locus_tag="ACGE4H_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFF8365892.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACGE4H_002513"
     tRNA            complement(3591..3663)
                     /locus_tag="ACGE4H_002513"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACGE4H_002514"
     CDS             complement(3709..4137)
                     /locus_tag="ACGE4H_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365893.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACGE4H_002515"
     CDS             complement(4197..4955)
                     /locus_tag="ACGE4H_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFF8365894.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1736 a          781 c          977 g         1483 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctg
//
LOCUS       DAXWLB010000176         4959 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXWLB010000176 DAXWLB010000000
VERSION     DAXWLB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.179"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            96..809
                     /gene="treR"
                     /locus_tag="ACGE4H_002516"
     CDS             96..809
                     /gene="treR"
                     /locus_tag="ACGE4H_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFF8365895.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4H_002517"
     CDS             886..1332
                     /gene="msrB"
                     /locus_tag="ACGE4H_002517"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFF8365896.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4H_002518"
     CDS             1502..2104
                     /gene="ruvA"
                     /locus_tag="ACGE4H_002518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFF8365897.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4H_002519"
     CDS             2117..3118
                     /gene="ruvB"
                     /locus_tag="ACGE4H_002519"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFF8365898.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            3147..3725
                     /locus_tag="ACGE4H_002520"
     CDS             3147..3725
                     /locus_tag="ACGE4H_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8365899.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            3777..4259
                     /locus_tag="ACGE4H_002521"
     CDS             3777..4259
                     /locus_tag="ACGE4H_002521"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFF8365900.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            4376..4750
                     /locus_tag="ACGE4H_002522"
     CDS             4376..4750
                     /locus_tag="ACGE4H_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365901.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         1702 a          850 c         1082 g         1325 t
ORIGIN      
        1 ttaaaataag cttttcttaa aaataggaac attgaaatac aaaaagagct gtagtatagt
       61 gaagaaatga tggaagaacg agtgagggaa aaaacatgaa taagttcaat gaaatttttt
      121 tagatttgga acagaaaatt ttagcgaaag aatatcctcc gcattcgctt ttacccagtg
      181 agaaccaatt gatcaaaatt tatggtgttt cacgagaaac aattagaaaa gcgctgaatc
      241 ttttgactac ggaaggttat attcaaaaaa aacaaggaaa aggttcgatc gtactggatc
      301 ggaatcgctt cgactttcct atttctggat tgactagcta taaagagcta caggagaccc
      361 aacgtattcc aagtgaaacg atcatccata cgttagaaga aacagaagtg acaaaagcat
      421 tgaacgagat cactggctgg gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa
      481 aaatcaatgg cgaagtagtt atattagaca ccgattatct attaaaggaa atcgttccta
      541 ttttaactcc agcacaagca caaggatcga tctatgaata ttttgaaaat gagctttctt
      601 tgacgattgc ttacgcgcaa aaggagatca cagtagaaga agtaacggaa atcatgaaaa
      661 cagcgatgga tcttcacgat gacacttatg cagtagtagt cagaagcctc gttcatctgg
      721 aagatacccg ctgtttcgag tattctgagt cggttcaccg attagataaa ttccgtttcg
      781 ttgagtttgc ccgacgccga aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc
      841 tgtccacttt ggcgctatga taggatcaca aagggggcat ttttaatgga taaaccaata
      901 agaccagaaa aagaagaact gaaagaaaag ttatcaccca tggcttatgc agtcacacaa
      961 gaaaatgcaa cagaacgacc atttagcggg aaatatgatg atttttacga aaaggggatc
     1021 tatgtagata tcgtcagcgg tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt
     1081 tgtggctggc catcattcag caaaccaata acgaaaagac aagtgcgcga aaaagccgat
     1141 ttttctcatg gcatgcatcg aatagaagtt cgaagcaaag aagcagattc tcatctcgga
     1201 catgtgttta ccgatggacc agtagatcaa ggtggtctac gttactgcat caattctgcc
     1261 tcactgaaat tcattccata tgaaaagatg gatgaattag gttatgggga attcaaatca
     1321 cttgtagaat aatggaaata cacaaagagc ggtacaatac ggggatttac tcgatcgtcc
     1381 gctctttttt tattcaagcg attgctattt gaaagaaacg ctccccagac tccttttttc
     1441 acaaggtgaa atgtgctaca ataaagggaa cgtatgtgtg caaaagaaag aggactagaa
     1501 tatgtatgaa tatctgacag gtgtggttac ctttatcaac ccctattaca tagtagtaga
     1561 aaccaatggt atcggctacc agatcgcact aggaaatcct taccgctaca gtagcaaact
     1621 aaatcaaaaa ataaaaattt atgtccatca ggtaatacgt gaagatgcgc atcttctgta
     1681 tggatttgat tcgctagaag aaaagcaact gtttttgcga ttagtaagtg tttcaggaat
     1741 cggtccgaaa agtgcccttg caatcatggc aagcgaagac cactccggtt tgattcaagc
     1801 agtagaatcc agtgatgtca cttatttgac gaaatttcca ggagtaggga aaaagacagc
     1861 acaacaaatg atcttggatc taaaaggaaa attcggtgag ctgagtatcg atacaccttt
     1921 tagtctattt gatgaagcaa acacaaaaga cgctacagct ctatctgaag caatggaagc
     1981 tctctcggct cttggttaca gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga
     2041 agtagataat cagacaacgg atgagtatct acgccaagca ctcaagctaa tgatgaaaaa
     2101 ataaggagga gaccaaatga cagatgaata cttgttatca ccagaatctg gcgaaaatga
     2161 actgagtttg gaaaaatctc ttcgaccaca gctgctttct caatatatcg gacagagaaa
     2221 agtcaaacaa gaacttcaga tttacatcga agctgccaga aatcgggaag aacctttaga
     2281 tcatgttctg ttgtacgggc ctcctggatt aggaaaaacg acgatggcca tggtcattgc
     2341 taatgagatg accgtaaata ttcgtacgac gagtggccct gctatcgaac gtccgggtga
     2401 cttagtagca atcttgaatg aactggaagc aggggatgtc ctgtttatcg atgagatcca
     2461 tcgattacct agagtggtag aagagatgct ttattcagct atggaagatt tctatgtaga
     2521 tatcatggta ggacaagggc caaccgcaca tccggttcat ttccctcttc ctccctttac
     2581 tttgattggc gcgacgacac gggcaggtat gctttctgca cctttacgag atcgatttgg
     2641 catcatttct catatggaat attatgacga acaggatctg caggagatcg ttttacgttc
     2701 ttcggatatt tttcaaacag agatcattga agaaggggca ttggaaattg ctcgccgatc
     2761 acgaggaacg cctagaatcg ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt
     2821 ccaaggagac gggaaaatcg atcgtcctat agcggatcag gcacttactt tgctacaggt
     2881 cgatcaggct ggattggatt atgttgacca gaaattgttg cgaacaatga tcgagttata
     2941 tggcggcgga ccagtagggt taagtacgct ttcagtaaac ataggagaag agcgtgagac
     3001 ggtagaagat atgtatgaac cgtatctgat ccaaaaagga tttttaaagc gtacacctag
     3061 agggcgaatt gctactgctt atgcctatga acattttggt tacgactatc atatatgatt
     3121 tttgctttta gattggagga aaatgaatga gtgatacaac acgtacgaaa caattgatca
     3181 aaaacgtatt gattgatttg tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa
     3241 tcgcaaatga agcaaaaatc aatcgacaaa cattttatta tcattttaca gataaatacg
     3301 atttgcttcg ttgggtctat tgtgaggatg cattgcatta tttagatcca caatgtcttt
     3361 cacttgataa ttgggaagag caagcattga aagtgctgaa agcaattgct gagaagaaag
     3421 aattttatgc aaatactgtg ctttctgacc cagaaatttt acaaaaagag ttttctcaac
     3481 tgatccaaaa atcatttatc aagatttttg aacaaatgga tgaagaaaga caactttctg
     3541 caagagacaa actattttat gctcgttttt tttcttacgg ttgcagtggt gtattagtca
     3601 attggatcac gaatgaatat gaagaaacac cgatggaaat cgctatgcag cttttccgtt
     3661 tagccaagga tacggaatta tattcttatc gtctttatgc aaaagaagag ttggaagaac
     3721 agtaatagaa aaattttttg ttggaaaagc aataatcgct aaaaggagat ggggaaatga
     3781 caaaaatttt atttgtttgt ttgggaaata tttgccgttc accgatggca gaaggcttgc
     3841 tgagaaaacg aatagcaata gaaggaaggg aagcagaatt ttttgtggat tctgctgcta
     3901 caagcacgta tgaagttgga aagacaccgc accctggaac gaaaaaaata ttaaaccaag
     3961 aaaaagtaga tatgacgaat atggttgctc ggcaaatcac accccacgat tttgaaactt
     4021 ttgattggat cattggcatg gatcaagaaa atgtagaaga actgaaacga agagcaccac
     4081 gctcagcgca ggggaaaatc catttgtttt taagtagcat tcctggaaaa gaaaaagaaa
     4141 atgttcccga cccatactat accaataact ttgatgagac atatcacttg atcacagaag
     4201 gcctgaatca ttggtggaat atttggattg acgaaaataa aataacaaac aatttgtaaa
     4261 gttgacgctt tctttttaag gcgatgttag agcatttcct tgtttttttg taaaatgtta
     4321 ggtaaaataa aattaattaa gtaacacttt cttagctaaa ggaggagtgc attcgatgaa
     4381 cgaagagaca gtatcgccaa tgaagaagct tacgaagtta gttactcata cttcgtatca
     4441 actttttgat tcttttgtaa atgacactga agaacatgct aagaatgtac gattgcaagg
     4501 aaagtttatt cttgagctgc aaaaagctgc tactgaacat agtctagtta tcttacaagt
     4561 aagagcgaat cgtttgagta gcaagtatga aacgatctca ggctgggtgg cgacaaaaac
     4621 ggtcgaaaaa gaccgagtga tgatccgcct acaaaacgaa gagcagcaga tccggatggt
     4681 tcctgtagaa caaattgaaa aaataacaat cctttcacca gcaggggaac aggaacgatt
     4741 atcaaaataa tttataccag atagaaaagc acgtatgagc ttgtaccatc aatcattact
     4801 aatcaaaaat aaccgaatgc tttaagctga tatcagtaaa acggaacgtt ttaggatgag
     4861 tatcacagca tgacagcatt gggttgatta gttgaatcga ttggatggta caagtttttt
     4921 tgataaaaaa aggtcctgct agccggggag ctagcagga
//
LOCUS       DAXWLB010000177         4949 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXWLB010000177 DAXWLB010000000
VERSION     DAXWLB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.180"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(128..1936)
                     /gene="pepF"
                     /locus_tag="ACGE4H_002523"
     CDS             complement(128..1936)
                     /gene="pepF"
                     /locus_tag="ACGE4H_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFF8365902.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2112..3110)
                     /locus_tag="ACGE4H_002524"
     CDS             complement(2112..3110)
                     /locus_tag="ACGE4H_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFF8365903.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3336..3989)
                     /locus_tag="ACGE4H_002525"
     CDS             complement(3336..3989)
                     /locus_tag="ACGE4H_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFF8365904.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4321..4719)
                     /gene="spxA"
                     /locus_tag="ACGE4H_002526"
     CDS             complement(4321..4719)
                     /gene="spxA"
                     /locus_tag="ACGE4H_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFF8365905.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1470 a         1028 c          762 g         1689 t
ORIGIN      
        1 aaaaagatgt tggtgaagtt caccaacatc aaaaagtata gagccttaaa aaaatacgtc
       61 gactccctta caagagagcc gacgtatttt tttcgtttct attcgagaaa atcaaaaata
      121 gaaagcctta taaacgatcg atcaattctt ctaattcatt tagacgttgt tcaaacatcg
      181 acattgcatc ttcaatatat gcagcctgtg tcatatcaac tcctgctttt ttcattactt
      241 ccacaggata atcgctgtta cctgctttta ggtaagccaa gtagttctct agtgcctctg
      301 gttcctggtt tagtattttc ttcgccagtg ctgaagcagc agaaaagcca gtcgagtatt
      361 ggaaaacata gtaattataa taaaaatgcg gtatccgtga ccattcgaat ttaatttctg
      421 ggtcttcttc gactgctgga ccataatatt ttgcattcaa tttaccataa ctatcactta
      481 ggtattcact agtcaatggc acaccttttt catcttccgt atgcatgaaa tgctcgaatt
      541 cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc atccaagtaa tggttgagta
      601 cataagcccg tacacgaggg tctttttctg tttccaataa atactccgtt aggatatttt
      661 cattcgttgt cgaagcaatt tctgctaaaa agatggagta gtcgccgtac acataaggct
      721 ggttcgaacg agtgaaataa ctatgaacac tatgtcccat ttcgtggaca agcgtaaata
      781 gctgatccag cgtatcatgc caattcaata aaatatatgg atttgtgtca tagcttcccg
      841 aagaataagc accgcttcgt ttccctttat tttcgacaac atcgatccaa cgttcagaga
      901 atgctttttc tacgatggcc atgtattctt cacccattgg tttcagtgct tctaaagctt
      961 tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc ccctaagacc ggtgtataaa
     1021 gatcatacat gtgcagtttt tccacttcta ataaacgttt ccgtaattcc atgtatcgat
     1081 gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt atcgtataca ctttcaggaa
     1141 tatgattatt gctcaaagac gcttctctgg cagagctgta attacggact ttcgctttaa
     1201 aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa tgtatttcta aattgttcgt
     1261 aaacactgta caacccctta aatgctgctt cgcgcaccct tcgatccgtg ctttctagca
     1321 actgaccata cacgccatga gataattgga ctatttcacc attttctcct tcaatcgttg
     1381 gaaaaactag atctgcatta ttcaaaacag caaatgtatc acttgaagca tcaaagattt
     1441 cacctgctcc agcaagaaga gattcctgct cagcagataa gacatgggct cgattatcta
     1501 ctatttgctg gatataatgg cgatagactt ccaattttgg ttcttctttg aaatattgcc
     1561 aaatctggtc atctgacaat tgcaatattt ctggttcaaa ccaggaaacg gcttcactaa
     1621 ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg gtaatctgta tttccagtgt
     1681 cttgatcgtt tttaaggtgc gcatatacat aaatgacttc agtttggcga taaaccctca
     1741 atacgaattc aatcgcattt aaaaattgag aagcgccttg atcaagtgtt cctttgtgtt
     1801 tttcagattg ttttaactct tctgataatt ccaaatattt ctcatcaaac tcttggtcgc
     1861 ttgaaaagat cttggtcaag tcccaagtta aattttcagg caattcttct cgttttggca
     1921 actgctttac ttccatgaaa tccactcctt ttcttccctt agctctatct atcaaaatag
     1981 atcatagtct aacatccaca tgtcacagca tctaactttt ctactcattc atgtgtctgc
     2041 ttccatcaat tttagcataa ctaaatcttg ttttacatag aaaaccgatt cctagcctat
     2101 ttttatactt actatttttt caaagaagac aagtgtatgc attcgttaaa aaactgctga
     2161 tagaccatct cttcatcaat cataggaaaa agccattccc ttttatgttc ttttatttgg
     2221 caaagccatg agttgaaatc aggaactttc tggttttcca aggcttcttc gtacaacatt
     2281 cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc gacttttttg atagatccac
     2341 gatgatagat gcaaaagatg tttatgacgc aagtagcatt gttcttgtat ggatactgta
     2401 ttcttttgct tgtgaataag ttggcgtgtc agccatttct tcccatcttc actcactttt
     2461 tctatttttt tcatttggtt cattttaatt tcgttactag tgaatatttc tttaaggtcc
     2521 ctactagaaa aagtccagca gctactgcaa aaggaaatat gccctgatac agtctctgta
     2581 atatgatgat acaaataaag cttttgtttc ttgagatcag ctttccagca atgcactcct
     2641 ctttttctat tatagtagca gcaacttttt tctatcaatg agaattgcga ttgtccaaga
     2701 aaagaattac caagtatcca ccatacttgg taactgttat tcaaataatt ttgggttctt
     2761 tcaataaatc tctggtgaga cagtctagaa cattggattt ctattgctag attcgccaac
     2821 aaaatatccg gcctttgccg caacctcggc aaataggctt ccaaaaatac tggttctact
     2881 gactgtccca gccaatccat gaagcattcc tttaactgta agtgctcttt tgattctctt
     2941 tcactaaaga ccgaacagtt atctgatttt tgatgggcaa aatgagcaca ttttttcttt
     3001 ccctttttca aaatcactct ttttcgacag ccacgacaat aatatttctt tcctttttct
     3061 gcatgtttag caaaacagca cttattatat tcattcattg caagcagcat ttttctccct
     3121 ctgaacttat ttacgtctaa gaatttattt acacgaaaaa agagggcgtg aaaaagctat
     3181 tctgcatcaa gtaataagaa cagctaaaca aaaatagttc ccaatatttt tcgtttggtt
     3241 gggcttgtta ccgcagatgc aagcttttga acacatttat acgaaaaaaa gactgcgaca
     3301 caatttttgt cacagtctct tactataatg ctgatttata caaaataaaa tttcgttcgc
     3361 tcgatagcat tacgcgccat cagacattgt ccatattcat tcaatgtttc tggtgtacga
     3421 tcgctctttt cagcaaactc caaaattcga gcaatctgat tctctacatc cgcttggttg
     3481 agattttcca tccagaagtg aacgactagg taatagcgct cttcgaataa atacaaatca
     3541 gtccaagcag actctagttc gacatctgtt gcaagttgga tcacttgttc aaaatcttca
     3601 aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc ttccagataa ttcttcctcc
     3661 ggatcgctgc caataatctg ctgacgaaga aagtcactca catcttctga atcaacagta
     3721 gaatcttctg attgagaaat ttcttcatcc ggaagatttt tactgataaa cagttctaat
     3781 ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc ttcctcggaa ctcctcttcg
     3841 atatcaactt cttccaaaat actataaaag aaattttcga tttctttgtg attgccaagt
     3901 aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat gaccaattag tacacggatc
     3961 gtattttcat taatatgttc catttccatt ttgctacacc tcactttgcc acaatgttta
     4021 atatagattc attgtagcga aatcagctta aatgtaaaga aaaaaaatct cgctgttaag
     4081 cgaatttaat aaaaccagcg gatttttcaa cctttcaagc tatcttattt aactataaca
     4141 tttttggcag acgaatacta tatagccgac ttttcagcta attttccctc tctatttttc
     4201 gtgcaatcat catcttctac taccttttta tacatctaat caagaatact ttttgtaaaa
     4261 acaaaaaaac ttggaaaaac tatctttgta atagtctttt ccaagtttcg tattatcctt
     4321 ctataatcca gccatcaatt gtgcttgacg taattctaat tgtcgtacat cgcgcggtag
     4381 gaaccgacga atttcatctt cattaaaacc aacttgcaga cgtttatcat caatcatgat
     4441 tggacggcga agcaatccag gattgttttg cacaagttcc aataattctt gtaacggcag
     4501 atcatctaaa tccatgttca gtttttggaa tactttggaa cgtgtcgaga tgatttcttc
     4561 tgttccgtct tctgtcattt gtaaaatcgc ttttaattca gaactgttca atggttctga
     4621 aaaaatatta cgttcaacaa atggaatctg atgttcttgc aaccacgcac gagcctttct
     4681 gcaggaagta caacttgggg aagtatataa tgttaacaac gtgcaccact ccttttcgtg
     4741 ataagaatca cttttttgta acagaagatc ataataatct cccatctgtt tctattatac
     4801 ataatcaact tttaaatagc tacctttttt tcataatttt tttaaaaaaa tgaaagttat
     4861 ctaaggttaa tatacagtaa aaccagcatt ctcgcttttt ttatcattta aaaattagag
     4921 ttttcaaatc taattcaaat tttctaaac
//
LOCUS       DAXWLB010000178         4857 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXWLB010000178 DAXWLB010000000
VERSION     DAXWLB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.181"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(29..1177)
                     /gene="nagA"
                     /locus_tag="ACGE4H_002527"
     CDS             complement(29..1177)
                     /gene="nagA"
                     /locus_tag="ACGE4H_002527"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFF8365906.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1253..2089)
                     /locus_tag="ACGE4H_002528"
     CDS             complement(1253..2089)
                     /locus_tag="ACGE4H_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8365907.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2273..3490)
                     /locus_tag="ACGE4H_002529"
     CDS             complement(2273..3490)
                     /locus_tag="ACGE4H_002529"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFF8365908.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3639..3800)
                     /locus_tag="ACGE4H_002530"
     CDS             complement(3639..3800)
                     /locus_tag="ACGE4H_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365909.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3873..3956
                     /locus_tag="ACGE4H_002531"
     tRNA            3873..3956
                     /locus_tag="ACGE4H_002531"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3907..3909,aa:Leu,seq:gag)
     gene            complement(4196..>4857)
                     /locus_tag="ACGE4H_002532"
     CDS             complement(4196..>4857)
                     /locus_tag="ACGE4H_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365910.1"
                     /translation="FPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTY
                     RKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGT
                     KLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSP
                     TVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVIN
                     R"
BASE COUNT         1480 a         1006 c          782 g         1589 t
ORIGIN      
        1 tttagtttta aggttaactt aaattatatt atgcgtggaa acgttccacg ccatctagat
       61 aagttgcttc caatgtcata tcagggttca atacgataaa gtctgcatcg cgtccttgag
      121 cgatttttcc acatgtatca tcgattttac agctgattgc tggaatcaga ctagccatca
      181 tgactgcttg ttctggtgtt gctaatcccc agtctacaac atttttaatt gcttctttca
      241 atttcaatat acttccagct aagttgccag attctagacg tgctgttcct tcttttacta
      301 ctactggaaa ttcgcctaaa ttgtaatttc catcaggcat cccaccggcc atcatacagt
      361 ctgtaattaa tgcgacatga tcatgtcctg ctttttccat taggattccc gcagcatttg
      421 gatgtacatg atggccatca caaatcaatt cagaaaagac atgttctaaa gataataacg
      481 cacccaccat acctggttcg cgatgattca atccacgcat accattgaaa gcatggacaa
      541 atacacttgc gcctgcttca acagcttttt gcgcttcttc tagtgtagca ttactgtgtc
      601 ctagagaaac aactactcct tcttcagtca cttgtttgac aaactcttct acaccttgac
      661 gttccggtgc taaagcaatt tttttgatta aaccacctga agcttcttgc cattcatgga
      721 aagtatccaa atcaggatcc ccgaaataac ttgggttttg tgcccctttg tgttcttctg
      781 tgaagaaagg accttcaaaa tagattcctt tgattttagc tcctggtgct tcctgataca
      841 ttttgccgat tgtctcagct acatctttta aacgttcttt gcttgacgtc aatgttgttg
      901 gtaaaaatga agtcactcca caagataaca gaccttcaga catcactttg atgccttctg
      961 catcattatc cattacatca tgattcatat agccgtgaat atgtgtatct accaaaccag
     1021 gagcaatcca tttgccgctt tggtcaatga tgttgacttc tccctcaggt agttctttgg
     1081 tgtattcacc aaaaagtcca tcggtaattt caagatagcc tgcatgtttg acacctgacg
     1141 ctaaaaagaa tttgtcagca tagataaaag ttctcattct attctctccc tttgtttttc
     1201 tacctctaaa gtactcttat ttagagaaga agtcaagaat ggtctacacc atttacaaat
     1261 tttttgaaat ttgtttcaca gcttgtgcta agccttcata gaatgctgct ctttctggtt
     1321 cttctttacg cttgtatatt aagcttttaa gcacaaataa atcattgagg taatagtagc
     1381 tattcttctc ttgagcccaa tagattcctt gattcacctg ttctaacgct tcgtcgattt
     1441 cattttgatg gaataagaaa tttccataat tataaaaaat ttgcgaaaat tcactttttg
     1501 atcgttcatc agctgattga tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt
     1561 cagccgtttt tcctgccaca ccatatattt ttccgataca actaatcagt tgtatttctg
     1621 tgtcagaaac atggattctt tctttttggc tagagtagct taatgcttct ttcaaataat
     1681 aaagggactg agagatattt tgacgcaacg tgaattcgca aacaccgaga taataataat
     1741 agcgttgaaa gtcagttgct tgatacagat tttcagaaat atcagaagat tttagtactt
     1801 gttctaactt ccgataatca cgtttttgga aacaactatc caacagttca aataatttat
     1861 cgtttgtccg agtagttttt cgagagagat tcatcacatg atcaatcgtt acccctagcc
     1921 gttgacaaat ctgcatcatc accaatacat tcggcagttc ctcattattt tcgatccgac
     1981 ttaatacact ttgtgaacaa atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt
     2041 ttcgaatctc tttaatgatc gttccaaagg actcgattaa cgtttccata agtatcctcc
     2101 ccaaaatatg cgtattcgca ttctattcgt attttacttt aggaagtcgc aaacttcaag
     2161 aaaagacttg caactaaata atatacttta gttatttaag aaaactccac tagaaagaga
     2221 gcctcagtgt gtttgtctgt ggccctccta ctgaaaaatt tggtttttaa aatcattttt
     2281 ggcgataggt tgctaggaat tctctcattt tcttagctgt ttgtttcaaa tcatccattt
     2341 taggaagata aacgattcga aaatgatctg gctggttcca attaaatcca ccaccatgta
     2401 caagtagaat atgatgttca tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat
     2461 tgaatcgctt tgtatcaatt tttgggaata tataaaaagc agcttttggt tttacggctg
     2521 aaagaccagg aatatcattg attgcattat agatatattc tctttgttca tagatccttc
     2581 cgcctggcag gagcagctca tcgacacttt gataaccgcc tagcgcggtt tggataattt
     2641 gctgagacaa tacattggaa cacaaacgca tggaggacaa catattcagc ccttctatat
     2701 agcctttgac gtgtgactta tctccactta ggaccatcca gcctactcga aatcctgcta
     2761 cacgatgaga tttggataga ccgttcagcg taacaacgaa caaatcaggc gctaatgtag
     2821 caatcggaat atgttctaga ccatccatca ctaagcgatc atagatttcg tcggaataaa
     2881 taatcaggtt attttgtcga gctacttcaa cgatttcttc taataattct tttggataaa
     2941 gtgccccagt aggattatta ggattaatga taacaatagc ttttgtccga ctcgttattt
     3001 ttgatttgat atcatcaata tcaggatacc attctgcctc ttcatcgcaa atataatgaa
     3061 caggtgtgcc tccagctaat gcaatagaag ccgtccacaa cggatagtcc ggcattggaa
     3121 ccagtacttc atctccattg tccagcaacc cctgcataca catcgtgatc aattcgctca
     3181 ctccgttgcc ggtgtagata tcattgatcg tcacattcgg aaaatgtttc agttggcaat
     3241 attgctcaat tgcttttcta gcagaaaaga tccccttgga atctgagtag ccttctgagt
     3301 cacgggcatt catgatcaaa tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg
     3361 cgggatttcc cgtgttcaat tttaaaatac taatgccctc ttcttgcatc cgctccgctt
     3421 cttctagaac gggaccgcga acatcgtaac tgacaccttc taatttgttg gatttttcaa
     3481 attctctcat cacttagcca atccctcatt tcaaaaagtt agaactttcc aaattttttg
     3541 ttatttacta tttcaatatc atactccatc ctagaaaatt ttgccactta tttaggaaaa
     3601 acgtaagtat taaaaaaaca caaacaagcg ttgagaactt aattgtcatg atagtcattc
     3661 catagattcg ctgcccaatc cattgcaatt ccacaaatga ttcctagcat aaaaccaagc
     3721 aaaaaacgtc cgaatgcaag tccaatcaat acacctaaga tcattcccag tcctgaaaat
     3781 tctccgtcat atttctccat cttaataagt cctcccattt ttgagcttga caactatgta
     3841 aaacctcgct ataattagtg tgttattatt acgcagatgt gttggaattg gcagacaagc
     3901 atgattgagg gtcatgtgag cgttagctcg tgtgggttcg agtcccatca tctgcactac
     3961 aactcgtgaa gttaattttg cttcacgagt ttttttgttt gaacgtttct ttatgccatt
     4021 atatacatat cactgtatcc tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt
     4081 gagtatatga aggtcggaaa caagctactc gtagaaactt gcaagtgtta aaacaattaa
     4141 tcgaaaaacg agtaaaatcc ctcacctaat actaaagcga gggatttttt gtatattatc
     4201 tgtttatcac cgttattata aaggtacaaa taccaagcac ggttaatcca aattgcataa
     4261 attcattttt ttgtacactc atgcttttca ttagctcttt aactgccatt agtactaaga
     4321 tggcgataac aacataaagt atatagtacg tccgcattcc ggaagcccat actgtgggag
     4381 aaaatcccat aattaacctt gaacaaaatc ccattatcat caaaataact aaaaaaatag
     4441 aagttttctt attgtcaaaa cttaaataaa ttccaacaat taaacatagt aataatgcca
     4501 agactactaa agtagctatc caagtacctg gataaaaaag tgacagtttc gttcctaact
     4561 ctgtaaagag attattcagg ttactactac tccaaatcat tgccctttta tttccattga
     4621 cgtaagtaaa gctttgaccc attgtattac caaaataaat aatcaggttt aagaaaaacg
     4681 gtaatgcagt caatatacgc acataatagt tttgacattt cctatatgtt agaaggaaaa
     4741 ttaagaaaaa tagcaataaa aataagatat tcatgtctaa aaaaaacggc tttccaaatg
     4801 atgaaatacc taaatctaac ttattaagaa agttgaaatt tttatattca ggaaacc
//
LOCUS       DAXWLB010000179         4791 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXWLB010000179 DAXWLB010000000
VERSION     DAXWLB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.182"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            2..409
                     /locus_tag="ACGE4H_002533"
     CDS             2..409
                     /locus_tag="ACGE4H_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365911.1"
                     /translation="MALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDHSERLV
                     ITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKKQLDY
                     TFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1581..2549
                     /locus_tag="ACGE4H_002534"
     CDS             1581..2549
                     /locus_tag="ACGE4H_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8365912.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            2559..3029
                     /locus_tag="ACGE4H_002535"
     CDS             2559..3029
                     /locus_tag="ACGE4H_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8365913.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3045..3215
                     /locus_tag="ACGE4H_002536"
     CDS             3045..3215
                     /locus_tag="ACGE4H_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365914.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            3625..4779
                     /locus_tag="ACGE4H_002537"
     CDS             3625..4779
                     /locus_tag="ACGE4H_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFF8365915.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
BASE COUNT         1825 a          773 c          793 g         1400 t
ORIGIN      
        1 cattgcttta gttatgggtc tagtgttagt tcttttctta actagttgtt caaaaaaaca
       61 agaaatacaa aagactgatc tgattccttt tctaggaact tggcaaacta aagatcattc
      121 agaacgtcta gtaatcacta atttaaatac ttcaaaaaat atagaacaat ataccatctt
      181 agtaaaagag aacaatcata aaattcaaat cattgataaa aatttaaata taaatgaaca
      241 agttttcatt tacaaagcaa caaataattt actaacactg cgctctaata aaaaacaatt
      301 agattacact tttagtttgg attctccaaa aaagctcgta tttattttga caacgaatga
      361 tgggacagaa gggacagcta aaccaattga attttttaaa gctgattgat gaatagttgt
      421 ttgatattac aaaacaataa tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg
      481 gctagcattt gctagccttt tttgtattgt ggagcgtggg ttttgagggg gattcaaggg
      541 ggagcttgcg cccctttgaa aagcggttat ttgcaccaaa tacgtaggta tttggctagc
      601 aaatatagag cttgccaaac ccttttagtg agttttgtag agagtgacct tataggcact
      661 ctcacattac gaacgatacg tccctcggac gtactgaaag ggtgggactt atgagcgaac
      721 taatgcaaaa tttggctagc aaaaaagcga aaaaatatat gctaataatc gagcgaaatg
      781 aacacttttt caagtgagcg aatccgccat gacaaagctg aaacagttta tttcagcttt
      841 gtcatggcgt gtgccgactt ttttaaaagt ctaggcacac gagcaaatat ctttatcaag
      901 ctctaatttg cgttttaagg cgtttcaaat gtctaagtat ataaatatat aagaatagag
      961 ttaagagtgc ttaaatcgaa ttttagaggg aattcagaca attttagggt tgttagggct
     1021 tgccctgacc gcctgtaagg cgctttaaga acaagataag ggatttatac taagactgtc
     1081 ttaaaacgtc tcaaacgaag gctcatagct tctcaaagag ttccttatga acagttttag
     1141 caaaggagcc ggaggcgact gcgcagtata atgtgagagc agagcgatca caccttttta
     1201 agcaactata gggagtaaga ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa
     1261 aagaagtggt tttttaggcg ggaaattttg tccccctctt ttataatctc tttttaaacc
     1321 tctttttaaa cctctttacc gtcggctgta tccttactct cccaagggaa tgacgctttt
     1381 atctacatcg ttttgtctgt ttatctacat cgttttgtct gtttatctac atcgttttgt
     1441 ctgtttatct acatcgtttt gtctgtttgt acattgaatt agtacatcga cgatgttaat
     1501 ataagatgta gataaacaga cattttgtat ttcataacgt ctgtatctct tgtgcgagta
     1561 agaaaggagc tattttaaat atgtctaatg aaatcgtaaa gtatcatcat gaattaaata
     1621 caattccgtt gcgaaaattt acaccagtcg aaatgaattt gttcttctct atcgtatcaa
     1681 gaatgcgtga tgtaggtgat aaaaaagtac aatttacatt cgagcaatta aaagatttaa
     1741 gcaattataa agcaactgca aatgttcgat tcatagatga tttagaaaca acgtatgaca
     1801 aattaatgga tttacgtttt ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg
     1861 ttttatttaa tcaatttaaa atcgacggaa aagctgatat tccttttgct gaaatacaag
     1921 ttcatgaaaa agccttaccc ttacttaata atcttgaaga atgggtgcgc tattctctcc
     1981 aacaattcaa tgaattagaa agcagttatt caaaaacaat gtttcgatta ctaaaacaat
     2041 acagaaccaa aggtttcgct tatttttcaa aagaagactt tcatgaatta cttgatattc
     2101 ctaaaagtta caaccaaggt acgatcgatc aacgtgtgtt caatccaata cgacaagaac
     2161 taaccgcaat ctttaaaggt ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc
     2221 ctgtcattgg ctaccaattc acatttaaac cagaaatcaa aaaagcagac gactttaaca
     2281 aacatgcacc aaccaaagaa gaacaaaatc gggctttaga actagctaat cgacaaactc
     2341 aacacgaaaa agaaactact tcattacaag aaagaattaa ggaacaaaag cgaaaattag
     2401 aacttttaca acagttagaa gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg
     2461 ataaacaaaa gttagacaac aattccgata ttcctctaga actaagaaaa gacttacttg
     2521 ataatcttca aaacttattt aaagactaag gagtattcat gacagataaa tcaataaaag
     2581 aatttgcaga agaactaggt gtcagcaaac aagccattag aaagtatttt gataaactgc
     2641 caaccaagtt gacacctaag ttgataggtg gaaaatacgt aatatcagca aaagctcaag
     2701 agtttataaa aaatagtatc aaccaaataa atgaagttga tactgaagtt gacagtttag
     2761 ttgatagtat tttaattgca gaattaaaag aaaaaaacaa acaaattgat catttacaag
     2821 gggtcataga aatacaacaa aaattactcg accagcaaca acaactcacc ttgcaggcaa
     2881 atcaacaaat cgacaaacta caaaaacggt tacaattagt ttatacggaa gaaacttcag
     2941 aaaatgaatc aactactctt tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga
     3001 agcaaaaatg gtggaaacta tggaaataac tggaggaaac attcatgaca agaaaaaagc
     3061 tcatatttgg ttctatttta ctaggaattc taggcttcag tccgatgtta ttgtcagtga
     3121 tcaataaagg aaataaacat ggtgaattga aattgaatcc aaattattac aacagtccta
     3181 acgaagagtt atttgtaaaa gaaacggata aataataaaa aaagactgct ctaacgagca
     3241 gcccaaaatg agattatcct agtttagcaa cataaaccat ttattagatg aaaatgagat
     3301 acaaacaaag cgtatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     3361 aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa agttgctttt
     3421 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     3481 ggcatagtgt acattatgtt aactaacgct ataaataacg attatgttcc caaaacaata
     3541 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat
     3601 accacttaaa tttaacacaa aggcgtgatt attttgaaac ccaaaaagaa aatttatcta
     3661 ggtctcctct tttccttgat gattggaatt tttagtatag gaattacaaa tacctatgct
     3721 tcagaaaatt ctaatttcaa tatcaactcc caaccaattg aagaacaagc aattaatttt
     3781 gcgaaaactg attttgaaca ctccataact tcaattcttt taatggaaaa taacaccgaa
     3841 actgatatta actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca
     3901 aatagttcta ttttctttcc agtattttca gacgctgata atacgattaa atatatttat
     3961 accgtcaaaa aaaatacaaa tggagaatat tcaggaagta tttcacaatt tttagcaaaa
     4021 gaacttcaaa atctagtaga tagtaatata acagatacaa ccttattttt tcaagataat
     4081 agcatttatt attccgataa tggacaaatt aaactacttt ggaaatcacc tttaagtaca
     4141 gaaaaaaatg gtgtttcttc ctttacagaa aaaaatgatc ttatatccgt aaatataaaa
     4201 aataaaatta aaaatttctc taaaaaacaa aattttcctg cgattaaacg tgtaaaaagt
     4261 gtctcctccg gagataactt tattatgatt aattggcgta tagcagaaac ccaaacaagt
     4321 tttccgtggt gtgctgctta cgtagaagct gctatcttat gtaacaaaac agatgtacat
     4381 gctacttcag ctagagccat tatgcattat acctatccta atttaaacaa cagtcaaatt
     4441 gaatcaaaaa gtcttaatca agatcaaata ataaaatatg caaattactg tggtgtttat
     4501 cctaaaaaag tagcacgaat actaaacctt tcagaggtcc aaacacaact aagaaaagga
     4561 aatgccattt atatgggaac tgctggttat ggaaattata gtaacagccg tcatgcactt
     4621 gcattatttg ggtgggtcaa tcaaggtggt tatcaaactt actatgtgtg gaatccttgg
     4681 tataactacc caactgtaat aagtgcaaat gcaggaccag ttacaattcc tgtacctgga
     4741 ggtggtttta cttgggacaa taccattaca aattggtaaa taaacccatt g
//
LOCUS       DAXWLB010000180         4760 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXWLB010000180 DAXWLB010000000
VERSION     DAXWLB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.183"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            107..349
                     /locus_tag="ACGE4H_002538"
     CDS             107..349
                     /locus_tag="ACGE4H_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFF8365916.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(437..583)
                     /locus_tag="ACGE4H_002539"
                     /pseudo
     CDS             complement(437..583)
                     /locus_tag="ACGE4H_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            640..954
                     /locus_tag="ACGE4H_002540"
                     /pseudo
     CDS             640..954
                     /locus_tag="ACGE4H_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            983..2242
                     /locus_tag="ACGE4H_002541"
     CDS             983..2242
                     /locus_tag="ACGE4H_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFF8365917.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            2295..3191
                     /locus_tag="ACGE4H_002542"
     CDS             2295..3191
                     /locus_tag="ACGE4H_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365918.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPESAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            3202..4071
                     /locus_tag="ACGE4H_002543"
     CDS             3202..4071
                     /locus_tag="ACGE4H_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8365919.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            4081..4756
                     /locus_tag="ACGE4H_002544"
                     /pseudo
     CDS             4081..4756
                     /locus_tag="ACGE4H_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         1509 a          730 c          992 g         1529 t
ORIGIN      
        1 atccagaata ttctaacata aatggagtat tcttagaatc aaaaggagaa agaagatggt
       61 ttatacttat acggagcaag aaaaaatgaa agtttgagac acattcatga atgatggaaa
      121 agtaaaacaa gtaccaagtt caacaaaaaa gaaaaacatc ctattgaaag aagtcttgaa
      181 acgctttgac catggagtga cttatacaga aacagaggtt aattcgatac tgctgaacgt
      241 attttctagt ggcgattatg tcgaacaaag acgttatcta atcacatttg gctttttcaa
      301 aagaagttca gacggtcggg catatcagat gatgggaatt gaaaattaga agttttaatt
      361 tatactcatt gaaaaaagtt caagtatgcg atattggttc tacaccgcat acttgaactt
      421 tttttgtcat ctgtatctat aatacaaagc caagaacgtt attcaaaata tggatagcta
      481 tgataaaagc taatgagcga ttcgcagtta aataagcaat cgtcagtaaa atcgttccag
      541 gagcgtacat gaaaaaatca attggatgcg ttacatgaat aaagataatt ctcttttatt
      601 tgtacgaata aataccaaaa cgtacctcct aaaattagat tcaaagaaga tagcaaagag
      661 gaaaaagata gttctgcagc agctatagcg gcatgtggat tgctagaact ttccaaacaa
      721 ctgcctgttt cagatcctcg tcatttcgat tacgaagaga tagcaatcaa aatcttggaa
      781 gaattacaga aaaactatac aacgaaacga gttaacggat caaacggtct gcttctccat
      841 gccgtttatg ataaaaacag cttaaaaggt gtagatgaat gtgtgatctg gggcgattac
      901 ttttatgtag aaggaatcac tcgtctagct aaaacatggt actgttactg gtgatgtata
      961 agagaagagg aggattcata taatgaagcg atggaaaaaa atgatgattg gatcaatagt
     1021 tttaggaatg tcactcgtat ttggtgcttg tggtaatcaa gaagacagta gtgaagaatc
     1081 agcaaatgaa attaccgttt cgacatggaa ttatgaaaca acacctgaat tcaaagcgct
     1141 ctttgaagca ttcgaaaaga aaacgggaat caaagtgaaa gcagtagata ttgcttcaga
     1201 tgattatgat acgaaactca ccacgatgct ttcttctgga gacacgacag atgtattgac
     1261 gatgaaaaat cttttgtcgt attcaaatta tgctttacga gatcagttag tcgatcaaac
     1321 agaacgaatt agagaattag acactgatgc tgctgaagaa acatatgaaa tgtatgatat
     1381 cgatggaaaa acatatgcat tgccatatcg aacggacttt tgggtactat attacaataa
     1441 gaaaatgttt gatgatgcag gaatcgaata tccaaaaaat ctaacttggg acgaatatga
     1501 agatttagca aaaaaactta gtaaaaatga cggacaagtt tacggagcat atcaacatat
     1561 ctggcgttcg acaatccaag ctattgcagc tgctcaaaac gaggcgaatc ttgttgaacc
     1621 agattatcaa tatatgactg attattatga tcgagcattg aggatgcaaa aagaaggcgc
     1681 gcagatggat tttggcacgg ctaaatcaac gaaagtaacc tatcagtctc aatttgaaga
     1741 acaaaaagca gcaatgatgt acatgggaac ttggtatatg gctggcatac tcgcaaacaa
     1801 agaagcaagt aagacagaag tagaatgggg aattacagct attcctcaga agaaaaaagg
     1861 agaatcggtc acgacgtttg gctcaccaac agcatttgct gtcaataaaa actcaaagaa
     1921 acaaaaagcg gcgcaagaat ttattgaatt tgcagccagt gaagaaggag caaaagtatt
     1981 agcaggagta ggagtagttc cttcatatcg tacggatgag attgatcagc tttattttaa
     2041 tctcgctgga atgccaacgg atgaaatttc taaaaaagct ttttctcctg atgaaatcaa
     2101 acttgaattt ccgattgata cgtatgggcc tgcaatcgat aagattttac aagaagaata
     2161 tgacttagtt ttggttggag atgaaacgcc ggaaaaagga acggtaaata tggagaaacg
     2221 tgtagctgcg gaaaaagaat aatcatgaaa ggagagaagc caagagataa aacactcttg
     2281 gtttcgtcat cgaaatggag aatagtataa ataagaaagc tacaaatcat ttgcgtagaa
     2341 aacgaacgtg gatcgcttat tcgtttatcg ccccgaattt cattggtttt ttcgttttta
     2401 cattaatacc aatcctgttt tcgcttgtac tagcatttat gaaatgggat tctttttcaa
     2461 cgccagaatc tgctggacta gataactttc gtaaaatggc caacgatgag acattttgga
     2521 tttctttgaa aaatactttt ttatataccg ctgctgtagt gcctggcaca cttgtttgtt
     2581 cgttagggct agctttacta ctaaatcaga aaattagagg gatgaagctt tttcgtacga
     2641 tatttttctt tccgtatgtt acttccttag tagcaattgc tgttgtttgg aatatgttgt
     2701 ttcatccaac agtgggacct attaatatct ttttaagacg atttattgaa catcctccag
     2761 gttggacttc aagttcaaat tgggcgcttt gggcaattgt aattgtcagt atttggcgag
     2821 ggatgggtta ttacatgatt ctataccttg caggactaca aagtatccaa aaagaactat
     2881 atgaagcgtc ttcgctagat ggagcaaacc gttggaaaca atttttgaat gtgacattac
     2941 cttcgttgag accaacaacc ttctttgtaa cgatcatgct tgtcatcaat tgtttcaagg
     3001 tgtttgacct tgtgcaagtg atgacggatg gcggccctgg tcgagcgaca aatgtgctgg
     3061 tttaccaaat ctacaatgaa gcattcaaaa aatttgattt tggttatgct tcagctattt
     3121 cacttgtttt gttcgtaatt gttttgggcg ttacggttgt tcaattttct tggaataagc
     3181 gtcgtactta ggaggtgtga tatggaagag agtgttcgaa atgaaagaaa aaaagcaaaa
     3241 attcgttcaa aaaacatgcc ctttattcaa tggttgggta tgattctgct gattgtatta
     3301 gctttattga cgatcattcc ttttttatgg atggtttctg cttcattcaa agcaaataat
     3361 gaagtgttaa cgattccgat tcaatggatc cctaaaactt ggcatttaga gaattatctg
     3421 gttatttggt ctcgtattcc tttagtgaca ttttttaaga atacagcttt tttaagcgtt
     3481 gtgatcacgt tgattcagtt gtttacttct agttttgctg tttacgggtt ttctgaacta
     3541 aattttaaag ggcgcgatat tttattttta gcatatatcg gcacgattgc tgtcccttgg
     3601 cagtcttata tgatcccgca gttcattatg atgagaaaag tgggtttaac agatacgttg
     3661 tggtcgttgg tgctattaca agcgttcaat gcttttggtg tgttcttgtt gaagcaatat
     3721 tacagtagta tcccagattc tttatgtgag agtgcgcgga tcgatggctt atcagagtgg
     3781 gaaatctatc gcaagatcat tttacctctt acaaaaccgg cgattgccag tttgacgatc
     3841 atcacattcg ttaatacatg gaacgattat atggggccat tcatttattt gtcttctacg
     3901 gaaaataaaa caatccagtt agggttgaaa atgtttattg ggctatttga tgcagagtac
     3961 gcattaatca tggctgcctc agttgtttct gttcttcctg ttgcttttat atttttagcg
     4021 atgcaaaaat attttgtaga agggatcgct tcatcaggaa tgaaggaata ggaggtgatt
     4081 atgaaagagc agcgttttga caaaaatgtt tatatggaat tcttttattg gttgtatcaa
     4141 tcattggcaa gtagcctttg tttttggtgt gtcaatatct tgtatatagc agctgcgttc
     4201 cttttgtcga tagatggaag gaatgtcttt tcctttattt tttctttact ttttattggt
     4261 ccaggttgca tcgtgatgat aggtatgaca gaagaattag caaaagaaaa aagtttttca
     4321 ccagtacgtt tgttcataaa gacgtttcgc caattttggc taaagggatt tttttattgg
     4381 ctatttgctt ggatagttag tgtgataatg atttttgatt gtttcttttt tatacgtttt
     4441 tcttatggaa aatggttgat tccgttgttt gttttacttg cttgttttac ttgcttgttt
     4501 atcagtttcc tttagcatca actgctggta ttttcaggtg agaaatccag cgtcgaaacc
     4561 aaatcaagtt ctacggatag cgttctacta tacgttgaaa aaatggtatg tgagtttact
     4621 agatttttta ctattgacaa gtttgtttct cttctttttc gtaaagcccc aatggtgtat
     4681 tctactaggt ccttctatcg tatttggttt gatttacttc aacaatcgta aattgatgag
     4741 aactatggat ctgtagactt
//
LOCUS       DAXWLB010000181         4745 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXWLB010000181 DAXWLB010000000
VERSION     DAXWLB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.184"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            16..2685
                     /locus_tag="ACGE4H_002545"
     CDS             16..2685
                     /locus_tag="ACGE4H_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFF8365920.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2776..3390)
                     /locus_tag="ACGE4H_002546"
     CDS             complement(2776..3390)
                     /locus_tag="ACGE4H_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFF8365921.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3508..3909
                     /locus_tag="ACGE4H_002547"
     CDS             3508..3909
                     /locus_tag="ACGE4H_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365922.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            4044..4382
                     /locus_tag="ACGE4H_002548"
     CDS             4044..4382
                     /locus_tag="ACGE4H_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFF8365923.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1467 a          811 c          884 g         1583 t
ORIGIN      
        1 tggtggaaaa taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac
       61 gcagcagcta gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg
      121 atctatcctg gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat
      181 tttcatgcca gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat
      241 gcttctctag gattgaatta tttatcttta atttcttact atctaggcgg gatcttcaca
      301 ccactggtgt tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg
      361 cttaaggtcg gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct
      421 cgctgggggc atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat
      481 tcggagatca tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt
      541 catcgtgtta tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt
      601 ttatccagtt tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc
      661 cgtctattga caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct
      721 ttactggcag gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca
      781 aatggcgagg cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat
      841 ctaatcatga aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc
      901 gtttacgcag gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta
      961 ccgttgaaaa ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat
     1021 cttgttccat tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt
     1081 tatgcctatc tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata
     1141 gaacaagacg atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg
     1201 gtggcatggg gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta
     1261 ttgacagtgg ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa
     1321 ctttcattca atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc
     1381 aatacgaaca gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta
     1441 tatactgatc cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac
     1501 tcgttttacc gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt
     1561 tacagtggta tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat
     1621 aaattaggtt tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg
     1681 ctaatggatg ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt
     1741 aaatatggct tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat
     1801 gcattgccac tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca
     1861 gataatttga ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat
     1921 tttacattct atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg
     1981 actggcgtca cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc
     2041 catgtgcctg caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta
     2101 gaaagttcta gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt
     2161 tctggtcaat actatgatct tggctattat cctcaagaga caacagtgac atttactgca
     2221 agtttttatg gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt
     2281 gtagcctatc aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact
     2341 ggcagaaaag cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt
     2401 ccttatgaca aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc
     2461 aaagacgcat tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac
     2521 ttgcctgaag gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt
     2581 ctttatgtct ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata
     2641 gaagaaaaac cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc
     2701 tgtgtcactc ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg
     2761 atttttggtg gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc
     2821 tacagtgatc ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg
     2881 atgaataagc ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc
     2941 agaaaaatct tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg
     3001 atgtgtttcg tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag
     3061 ctgcggataa cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag
     3121 atcatttcta tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat
     3181 ttcatcgaca ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg
     3241 attgtatttt agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt
     3301 aatatccgaa ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac
     3361 acttaatatt tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa
     3421 taacggtgta catttttaaa aggagatgta taatcgagat ttagaaagcg ttttcctaaa
     3481 aatttaatga attaggaagt gattactatg aaaaaattat ttagcgcact ttttattctg
     3541 ttatttggct ttattggtat tgggacttct ggaaatatag ttaaagctag cacgatggtt
     3601 gaacaaacaa gtgaaatggc aaaaactttt agtcaattaa caccagaaga acaaagttac
     3661 ttcaaatcca aaggatttag tgataaagat actttttaca ctcaaaagat agagcctcaa
     3721 gatccgaaaa cacgtttttc tgcaaatgta ttgcggttaa ccgcatctac taaaaaaatt
     3781 actaatacgc aggggcggac agaatatatt ataacaagtc ccaattcggc gatgtatcgt
     3841 atttctacac gcttgagttt aggtagtgca aaatcatata ctcaaacagt aaatctagct
     3901 aattcttaag gttatggtgg aggaatgtat tttacattta ctgggaaaag acaatattac
     3961 agtgtaaaat tagctatgca agtttataat acatttggtg tagggtcagt ctcttgcact
     4021 gcgggaggat tgacaatagg aaaatgataa tttcttacta tgtcttaaat agaagtgatg
     4081 atgaaggact cttcgttttt caatcattac atgaggatac gatgctaaaa gagttttata
     4141 atcggattaa gaataaacag atagaaacaa atttatattt tttaagtgac tctatcttgc
     4201 cttttattag caaccctaaa attcttaact tgttgcagac aaaagatttg aaaagtttac
     4261 cgattattgt cattgatgag gaaattgtaa aatatggcgc ttatctaagc gtagaagaat
     4321 tagaaaaatt aactaacatt ggaatgagta ttcaggagga taatggggta catggaacat
     4381 agaatgaata tgcacagttg agaacggaat ttgacattta tttagaccaa cagggtatac
     4441 cctgttggtc tttttgtgcg ttcatgatat acttggtgtc atttttatat ttaaatggca
     4501 caaatatttc taattatttg tcatttaacg ctgaaaattt ttgctattta tctaaaaaag
     4561 tgacaggata ctcatttggc cttcatagaa ttttctaaat atgaatcatc aatgaattta
     4621 attaaaaaaa taagtagggc aaaatataaa tgtttttgcc ttacttgttt ccatcagttt
     4681 agttttaaat tgataagtta tatagttatg gttgaatgtg tgtctcgcat attagtattt
     4741 ttacc
//
LOCUS       DAXWLB010000182         4744 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXWLB010000182 DAXWLB010000000
VERSION     DAXWLB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.185"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            25..696
                     /locus_tag="ACGE4H_002549"
     CDS             25..696
                     /locus_tag="ACGE4H_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8365924.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACGE4H_002550"
     CDS             755..1195
                     /locus_tag="ACGE4H_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365925.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACGE4H_002551"
     CDS             1207..1875
                     /locus_tag="ACGE4H_002551"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFF8365926.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACGE4H_002552"
     CDS             1939..2562
                     /locus_tag="ACGE4H_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFF8365927.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACGE4H_002553"
     CDS             2618..3004
                     /locus_tag="ACGE4H_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFF8365928.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACGE4H_002554"
     CDS             3217..4608
                     /locus_tag="ACGE4H_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFF8365929.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DAXWLB010000183         4689 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXWLB010000183 DAXWLB010000000
VERSION     DAXWLB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.186"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(2..472)
                     /locus_tag="ACGE4H_002555"
     CDS             complement(2..472)
                     /locus_tag="ACGE4H_002555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFF8365930.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(631..1089)
                     /locus_tag="ACGE4H_002556"
     CDS             complement(631..1089)
                     /locus_tag="ACGE4H_002556"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFF8365931.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1260..2405)
                     /locus_tag="ACGE4H_002557"
     CDS             complement(1260..2405)
                     /locus_tag="ACGE4H_002557"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFF8365932.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2418..3335)
                     /locus_tag="ACGE4H_002558"
     CDS             complement(2418..3335)
                     /locus_tag="ACGE4H_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFF8365933.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3763..4326
                     /gene="efp"
                     /locus_tag="ACGE4H_002559"
     CDS             3763..4326
                     /gene="efp"
                     /locus_tag="ACGE4H_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFF8365934.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4427..4597
                     /locus_tag="ACGE4H_002560"
     CDS             4427..4597
                     /locus_tag="ACGE4H_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365935.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1464 a          850 c          881 g         1494 t
ORIGIN      
        1 gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc
       61 gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt
      121 tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc
      181 catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt
      241 ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga
      301 taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg
      361 ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca
      421 atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt
      481 ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc
      541 cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata
      601 ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg
      661 ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca
      721 gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt
      781 tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa
      841 gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag
      901 ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg
      961 ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc
     1021 agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt
     1081 cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag
     1141 aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc
     1201 aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc
     1261 tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca
     1321 attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt
     1381 ggaatagacg catgagtcat gacagaagga atttccacta aactttctac gccacctaaa
     1441 ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt
     1501 tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg
     1561 agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga
     1621 aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg
     1681 attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg
     1741 taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca
     1801 tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct
     1861 aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt
     1921 tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt
     1981 gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta
     2041 ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg
     2101 aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa
     2161 aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc
     2221 aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca
     2281 tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca
     2341 ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg
     2401 tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct
     2461 ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct
     2521 gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta
     2581 gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa
     2641 agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca
     2701 ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct
     2761 ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta
     2821 tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca
     2881 ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt
     2941 tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt
     3001 gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa
     3061 accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca
     3121 gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc
     3181 aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca
     3241 taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg
     3301 gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata
     3361 aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat
     3421 aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag
     3481 tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt
     3541 catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca
     3601 aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca
     3661 aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata
     3721 caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat
     3781 ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc
     3841 caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca
     3901 ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac
     3961 acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa
     4021 acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta
     4081 gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca
     4141 acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact
     4201 ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc
     4261 gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca
     4321 aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca
     4381 gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa
     4441 agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac
     4501 tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc
     4561 gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta
     4621 tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt
     4681 tttaaaaaa
//
LOCUS       DAXWLB010000184         4469 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXWLB010000184 DAXWLB010000000
VERSION     DAXWLB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.187"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..253
                     /locus_tag="ACGE4H_002561"
     CDS             <1..253
                     /locus_tag="ACGE4H_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFF8365936.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNPVGSGDATVAGLTAAIQQGLSDE
                     AVLKQGNVLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            266..1252
                     /gene="lacD"
                     /locus_tag="ACGE4H_002562"
     CDS             266..1252
                     /gene="lacD"
                     /locus_tag="ACGE4H_002562"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFF8365937.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            1708..2376
                     /locus_tag="ACGE4H_002563"
     CDS             1708..2376
                     /locus_tag="ACGE4H_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFF8365938.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            2367..3500
                     /locus_tag="ACGE4H_002564"
     CDS             2367..3500
                     /locus_tag="ACGE4H_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8365939.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3795..4220)
                     /locus_tag="ACGE4H_002565"
     CDS             complement(3795..4220)
                     /locus_tag="ACGE4H_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8365940.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWSYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT         1422 a          761 c          977 g         1309 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaacccagtt ggctctggtg atgcgaccgt tgccggattg accgctgcga ttcaacaagg
      121 tctttcggat gaagccgtgt taaaacaggg taatgtttta ggaatgctga atgctcagga
      181 agcgacgact ggctatgtcc aattagccaa ttatcaaaaa ctatatgatc aaatcttagt
      241 ggagaaagtg taggaggata cggaaatgaa cgaagaaaaa cgtcaatata tcaagcaact
      301 atgcaatgaa gatggagtga ttggggcctt agcaattgat caacgcgggg ctttgaagaa
      361 gatgatcaca aaattcaagg gcagcgaagc agaagaatca gaaatcgttg aatttaaaga
      421 aattgtttct tcacaattaa cgaaatatgc ctcatcgatt ttattagatc ccgaatatgg
      481 cttgccagca gctgacgtta gagcaaaaaa tgccggcttg ctgttagctt atgaaaaaac
      541 tggttacgat gcgtcaactc ccggccgttt accagattta ttatcgatct ggtcagttaa
      601 acgtttaaaa gaagcaggcg ccgatgcttg taagttccta ctttactatg atgtcgatga
      661 agcagaagaa atcaataatc aaaaacatgc ctttattgaa cgaatcggct cagaatgtca
      721 ggctgaaaac ttacccttct tcttggagct tgtttcttat gacgcgaaca tctctgatgc
      781 gacttcgaaa gaatacgcca ttaagaaacc tcataaagtc aacgatatga tgaaggaatt
      841 ttctgatcca cgttatggcg tagatgtgtt aaaggtggaa gtacccgtca atatgaatta
      901 tgtggaaggc tatgggacag acgaagtcgt gtacacaaag gcacaagcgg ctaacttctt
      961 caaggaacag gctgaagcca ctaacctgcc atttattttc ctaagcgccg gtgtaaaagc
     1021 agagttgttc caagaaacac tgcgcttcgc gaaaaatgcc ggctcaacct tcaatggtgt
     1081 actttgcggc cgtgcgactt gggcagatgg tgtagaaagc tatatcgttg atggaaaaga
     1141 agcagcgatg gagtggatga atacgcaagg gcgtaaaaat attgaagaat taaatgccgt
     1201 attgaaagaa actgctacac cggtggaagt gttaacaagc tcactatcat aagaaatcag
     1261 actgtgaagc aaaattgctt gcagtctttt tttatacata aagcgtaaaa tcatgctgca
     1321 ttattagggg aacgaattct aaaatagtaa gtgagttaaa acgaaggggt ttttctttag
     1381 agagattttc ttacatgtgg tacttaatga cttgatggca attttcagta ttctaacgag
     1441 gcttattggt cataaaccaa cgaactttga aaagtatata caagaaaacc aagcaaatag
     1501 aaagtagtga gactggagga aaggttccag tctttttcta ttctaggtat tttcaaacag
     1561 taaaatcatt cttaattatg tgttttttaa cagaatgtat gaatttcttc aaatcttcac
     1621 aaaaagaacg tggactttga agaagagcgt gtactatgct aaaaagcata agtgtaatta
     1681 ctaaatccgt atgaggagtg aatatacatg cgtgtactgg tggttgagga caatcgtgag
     1741 ctggttttat ccgtaaaaaa gggcctagag caggagaata tttctgtaga tgtagcttat
     1801 gatggcgagt caggagaaga gaaggccttc atcaattcat acgatgggat actactagat
     1861 ttaaatctgc ctaaaaaaga tggattatct gttttacaaa gtctccgtgc tgaagaagtc
     1921 gaaagtccag ttatcattat tactgcacgt gatgagattg aagaacgagc aaaaggcttg
     1981 gattttggag cagatgatta tttagtcaaa ccctttgagt tactagaatt aaaagcacga
     2041 atacgcgcgg ttattcgtcg ttatcatgga cgtgcgctgc cgcagatttc aattggtgct
     2101 ttgaagattg atccattagg tcgaagggtc agctatcaaa agacagaggt ggtcctgaaa
     2161 cccaaggaat ttgatatctt actgtgcatc gctcagcgtc atccagcggt tgtttcgtct
     2221 gaggaaattt ctgaacatgt ctacgatgaa gattttgatc ctttttcgtc ggttctgcga
     2281 gtgcatctgg ctcgtctaaa aaagaaactg gctgaagcag ctgaaaaaga ggtcttaaaa
     2341 accattcgag caaaggggta ccagctatgc tattaaattt taaaatgacg ttaacgatgc
     2401 tggctttttc actgttggtg acaggcgtga ttttagtgag tttcttcttt ctgagaaata
     2461 atttggaggg aatcagtatc gcatctacct taggtgaaga tgtttatttt atggaagagg
     2521 gtaagacggt tcaagcagag acgcagatgg aagcttcgaa atcttccaac aatcaattta
     2581 tgactatttc gatgatagat aaagcttacc aggacgaact atttaaatat ttaccagcga
     2641 tggtgctggt tatttgttct gccgtcatgt tattaacgtt tattctttgg ttggttttac
     2701 gcaaaattta cagtaatcaa atcttagcga ttacgcgtga tttaacgttt attgatcaag
     2761 ttcctaaaaa tactgtgaag gacgaggcat taaatgcggc ttttaataag ctaaaggata
     2821 agttcgatgg tcatttggag gattatcgac gattaaatag ttatctgacc cacgagcaga
     2881 aaaatgccat cgctattttg cgtacgaatc tggaaataga acggcaacat gccgatcaag
     2941 gaaatttaat gcttttagat cggatgaccg acagtattga cgatattttg acattatcca
     3001 ataactcaga ggatcttagc acggaagtcg tagacgtttc actgatctgt gcggaagttt
     3061 gtgatgccta caaaatgaat tatccgaatc ttttgtttgc gtttactgaa gatcaaccaa
     3121 ccctgattca tggacgagat cgttggattt atcgggcggt ttcgaattta gttgacaatg
     3181 caatcaaata tggtgaagga aaactggtaa ttgtcaccat ttcaaaccaa aagggtagcg
     3241 tgattattga agtaaaagat cagggaattg gcattcctca ggagaaacag gcatcaattt
     3301 tttctcatca gtaccgagtg catgggctca agcaggatgg cttacctaaa acaatggact
     3361 ttagggacac ggatgaaacc tttattcaat ccgttgcctc gaaaagaaac catattccaa
     3421 gaaaatcatt aaattatcga acaccattgg aagtattttt gagctgcata gataaagaca
     3481 tattgtctag cttaatttga caaatcagat gtctataaaa ttcgttagaa aatacaattt
     3541 atagatatcc aattgatttg taaacaaaaa ggagttgtca actaagccac tcctttttgt
     3601 tgtaattttt cccaataaga aagtctacta ctttaggaag ataatagtaa gatagaaatg
     3661 ttgcccccaa aattattatc cagctggata taattaatca aataaaaagg atcaaaaatg
     3721 tagtgacccc caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct
     3781 ctaaaatgat atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc
     3841 cagtttaatt tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct
     3901 gattttaatt cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag
     3961 ccaaaaaaat tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga
     4021 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga
     4081 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
     4141 ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg
     4201 gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg
     4261 gtatgtaacg gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg
     4321 tagggcaaac aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta
     4381 gggtacaact atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa
     4441 gtcaaaatat tttttatgag ttatttata
//
LOCUS       DAXWLB010000185         4419 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXWLB010000185 DAXWLB010000000
VERSION     DAXWLB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.188"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            60..914
                     /locus_tag="ACGE4H_002566"
     CDS             60..914
                     /locus_tag="ACGE4H_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFF8365941.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1011..3260
                     /locus_tag="ACGE4H_002567"
     CDS             1011..3260
                     /locus_tag="ACGE4H_002567"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFF8365942.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3263..>4419
                     /locus_tag="ACGE4H_002568"
     CDS             3263..>4419
                     /locus_tag="ACGE4H_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFF8365943.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          626 c          807 g         1430 t
ORIGIN      
        1 tgtaataata taaacataat aaaacaaaaa ttatccaaat ctaacatagg agggtggata
       61 tggaaaattc tatatattta gtaacagaac ctaacactca gctgaaagat tgttacttta
      121 atttttgtgg gtattcaaaa acagaaccta gacatagttt tggaccagct attcgagaac
      181 aatacttaat acacatagtg ctagaaggtg aaggctatta ttcaattaat gatcaaaaat
      241 attttctgaa gaaaggccaa ggatttgtta tcccaccagg agtatctact ttttatcaag
      301 cagatgaaaa aaatccgtgg tcatatgtat ggttgggttt aagtggcaat cttgtagaat
      361 actatctaaa agaaattggt gttggaaaga atcatcttac ctttaaagtc gaaaatttaa
      421 attcatttaa aggattaatt tttgaaactt tatcgtatga tcaggacaat ctagtcagtg
      481 aattgtccag acaaaaacaa gtttataaat ttttagaact cctcttaaat tcagctagtt
      541 atctgaacga agggataatt actaaaaaga tgaatccttt tgttcgtcaa acaattgaaa
      601 taatacgtga gaatgcgtat caaaaattat ctgttaaaga tattgctgtc agtctatcga
      661 tacacgaaaa ttatttatca cgattgttta agaatgatat gggaatatca ataaaagagt
      721 atattagtga aatcaaaatt tctattgcaa acgatctgtt atcatcaact gaatattcaa
      781 tccagcaaat cagtgacaag ataggattct caacagtaca atctttttct aaagccttta
      841 agaaagcaaa tggtgtttca ccgggagtat atcgaaaacg tagattaggt ctaggaatca
      901 ttaatggtga ataaactaaa catttgattt tgataaatat tcgttacttt aaacgcttac
      961 tatttatgtg agcgtttttt ataatgcaat aaagaaacgg aggagagctt atgtataagg
     1021 ataatgaaat gaatattatt tatcgatcag aaaaccaaca atttcatttg tttaatcagg
     1081 agataagtta catttttaag ataccggaag atggtaaatt attgcattta tattatggtg
     1141 agaacttacc agataatgac tatagtcatc taattgagat gcatcatcgg cctatgacaa
     1201 cctatagaaa tgaaaacgat ttgctatatt cgttagagca cttaaaacaa gaatttcctg
     1261 aatatggtac aacagatttt agacatcctg ctatttcatt actacagaaa aatggctctc
     1321 gcttatcaga ttttgtctat caagggtacg aagtggaaga aggaaaacct aaactagaag
     1381 gtttacctgc tacctatgtt gataataatg atgaaagcaa aacattgaaa gtttatattt
     1441 tagattcagt tacaaatgtt caagtagaat tattatatac aatttataga gattatcctg
     1501 ttattactcg tagcgcgcga gttatgaata aaggaaaaga tattcaaata atcgagaata
     1561 ttagcagttt atcattggat ttacccgatg caaattatga atggcaacaa ttatctggtg
     1621 cttggggtag agaaagaact attaagtcgc gtactttaca acaaggaatt caatcaatcg
     1681 aatctactcg aggtatttca agccatcagc ataacccatt tgttacttta aaaagaaaaa
     1741 atactgatga aaatcaagga gaagctattg gtgcagcact agtttactca ggaaacttct
     1801 taattcaagc agaagtagat acatgggatg ttactagact acaagttggc atcaatccat
     1861 ttggatttaa ttggaaactc aatccaggag aaatatttac agcaccagaa gcgattctgg
     1921 tttattcaag taagggttta aatgccatga gtcaaacttt tcatcaacta tttagaaaac
     1981 gcttagctcg tggtaagtgg cgagaaaaag accgtcctgt cttaattaat aattgggaag
     2041 caacatattt tgattttgac gaagaaaagt tggtaaatat tgctaaaaaa gcatttgatg
     2101 ttggtattga attatttgta ttagatgatg gctggtttgg agaaagagaa aacgattgtg
     2161 caggattagg agattggcat gtaaatccta aaagactacc tagaggaatc ggcagccttt
     2221 cagaaaaaat tcgtagtttt ggtatgaaat ttggtttatg gtttgaacct gaaatggtga
     2281 ataaggatag tgagttatat cgcacacatc cgaattggat tatttctaca cctaatcgaa
     2341 aacctaatca tggtaggaac caatatgtat taaattttgg gttagatgag gttgttgaaa
     2401 atattttcga tcaaatgtgt aaaatcattg atgaatcaaa cttagattac attaagtggg
     2461 atatgaatcg tccattgact gatgtatttg acagtcactt gtcagcagat caacaaggag
     2521 aagtattcca cagatatgta ctaggcgtat atcgcttata tgaaaaactt ataacaaaat
     2581 atcctaacat actatttgag tcttgttcat caggtggtgg gcgctttgat cctggaatgc
     2641 ttcactatgc acctcaagct tgggctagtg atgattcaga tgcaattgaa aggttaaaaa
     2701 ttcaatatgg aactagtatg ctgtatcctc tttcgtctat tggtgctcat gtatcaattg
     2761 ttccaaatca tcaaacaaac cgtataacac ctataaaaac tagaggaaat gtagcttttt
     2821 ttggtgcatt tggatatgag cttgacttaa atgaattaag tgaaggcgat ttgaggattg
     2881 taaaagaaca aattgccttt tataaaaaat atagaaccat aattcataat ggtacttttt
     2941 atagactatt atcaccattc gatgatgata atcaaaccgc gtggatggtt gttagtcatg
     3001 ataagaaaac ggctattgtt gctcattata aaacattaaa tgaggtaaat gctccttacc
     3061 gccgactgaa attaaaaggg ttagataatg acggacttta taatgtagga caaaatagtt
     3121 tgcgatctgg aatggaattg atgagagcag gactagtctg ttcagatgca tctagtggac
     3181 aaataaatga tcagaatcga cgcccagaaa catttgattt tgattccaag atttgggtta
     3241 taacacagga ggaaaagtag agatgaataa ccaaaaaagc ttacttcaaa aaggagcaat
     3301 cttaagtatt tcactaatgt taacaagctc tcaagcaatt aatggtgtta ttccacaaat
     3361 gaaacagtct ttggggatat cacaaagtca aagtgaactt ttaggaactg caccaagtat
     3421 aactgttatt ttgtttatct tattatctag ttactttgcc aaaagaatag gaatgaaaaa
     3481 gacaataatt ttaggtttat tattagctgg tataggagga attattccag tatttacagc
     3541 taccttccaa accgttctta ttagtcgagt aatattaggt gccggtttag gagtatataa
     3601 ttcattggct gtgacatata tcagctcttt atactctggc aatactagag caacgctatt
     3661 aggtattaga aattcaatgg aagctttagg tcagacaatc cttatttttc ttgctggagt
     3721 tttagtgaac attaattgga cggcttcgtt tttagtctat gcaattgcgt ttcctgtagc
     3781 tctcttattc gcgctaaaag ttcctgaaat tagagatgaa aatagtgaca tacctgaaaa
     3841 tcatgtaaaa gaaaagatga atccaatggt ttttgcccta gttctatttg caatcttact
     3901 cgtaatgaat agtattgcta tttctgtacg atttgcatca attgcaactg aaattaaagg
     3961 ggtaaacttt aatgctagta actatttagc tctaatgcct atcttaggaa tactagcagg
     4021 atttatgttt ggaccaataa ataagtggat aggaaaagga accctatact tgggaattat
     4081 attctttata atttcaaatt tgttaattgc tgtgtcaaac ggtaatatga cgttcctgct
     4141 aacgggttta tttttatcaa gtataacagg atcatggtgc ttcccattta tctttagtaa
     4201 cttagataaa gtcactacaa agaatacaat taattttgct acttcattga tttttattgg
     4261 ctgtaatatt ggtaatttta tagctcctat tgctatgcaa ttagcacaat ttctaacaag
     4321 aagtacaaat ttgactgctc cattttttgt attcgcagga atatttattg tgatcctatt
     4381 tgtaacattt tttgcgacaa ataaaaaaaa tactagagt
//
LOCUS       DAXWLB010000186         4402 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXWLB010000186 DAXWLB010000000
VERSION     DAXWLB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.189"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     regulatory      65..231
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            379..936
                     /locus_tag="ACGE4H_002569"
                     /pseudo
     CDS             379..936
                     /locus_tag="ACGE4H_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            994..3033
                     /locus_tag="ACGE4H_002570"
     CDS             994..3033
                     /locus_tag="ACGE4H_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFF8365944.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLYAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3142..4293)
                     /locus_tag="ACGE4H_002571"
     CDS             complement(3142..4293)
                     /locus_tag="ACGE4H_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFF8365945.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1596 a          813 c          860 g         1133 t
ORIGIN      
        1 catttcaaat tccattgcct aatcattagg aaaagtctat catgaactcg taaatagaat
       61 atagtaataa ccttgttagg tgaggctcct atacaaacat aggctgttgc ccagaaacgt
      121 cgaaagtcgc caatgggtaa aacagaaact gttggattaa ggctttttcc aatagctaag
      181 aatgttttct ttacgttgta tagtgctaaa actcggacga tgagatgatg ctaggtcaga
      241 attcttgttc gaaaaggttt ctgacctttt ctttatattt ttgtgactga cttatttgat
      301 gagacaaact cgctcctcac ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa
      361 aaaagatcga ttggtggagg tcgctcggat tcacttctgg ttgcacaata caaccagaaa
      421 acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac
      481 ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca
      541 ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt
      601 atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta
      661 gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc
      721 cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag
      781 caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc
      841 aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg
      901 gattgtggta tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa
      961 taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa
     1021 aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt
     1081 tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt
     1141 gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca
     1201 aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat
     1261 atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa
     1321 gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg
     1381 aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg
     1441 caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa
     1501 gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa
     1561 ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat
     1621 atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca
     1681 caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca
     1741 gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa
     1801 gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat
     1861 ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag
     1921 gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa
     1981 aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg
     2041 aaggcacaaa aaatagcttt tgacagtcta ggaggaaaag ctgcatcaac tgttgcgaca
     2101 acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa
     2161 atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca
     2221 ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat gattacagca
     2281 gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt
     2341 aaatggcaaa aagacagttc ttggggatct tttaaaacaa cgcgtgtcag tagtgatgta
     2401 tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa
     2461 acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa
     2521 gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac
     2581 tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat tactcctatc
     2641 cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg
     2701 attgcagata aagagacaaa agataagaag aatgtcatca gcgaaacagc agtacaaacg
     2761 atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca ttctctttct
     2821 gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat
     2881 gtaaaggggc aagagaacag tttcttgtat gctttcaatc ttgataacca aggttatatg
     2941 atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg agcatcggaa
     3001 ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt acagtaaaag
     3061 gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt
     3121 cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac
     3181 aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg
     3241 ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga
     3301 aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc
     3361 aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag
     3421 tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg
     3481 tataaatact gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt
     3541 ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc
     3601 cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc
     3661 gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat
     3721 aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc
     3781 ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac
     3841 cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa
     3901 tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc
     3961 atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat
     4021 cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact
     4081 ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag
     4141 aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg
     4201 tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat
     4261 caatatcagg cataaaatac caataaattc cattctcatc attcctttgt ataaaatata
     4321 gcccgaactt agcataacat acattattga aaaaatagga aaatatactc acaaaaaaac
     4381 caacaatcca aaagattttt tt
//
LOCUS       DAXWLB010000187         4282 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXWLB010000187 DAXWLB010000000
VERSION     DAXWLB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.190"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(37..1257)
                     /locus_tag="ACGE4H_002572"
     CDS             complement(37..1257)
                     /locus_tag="ACGE4H_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFF8365946.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACGE4H_002573"
     CDS             complement(1277..1888)
                     /locus_tag="ACGE4H_002573"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFF8365947.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACGE4H_002574"
     CDS             complement(1931..2863)
                     /locus_tag="ACGE4H_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFF8365948.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACGE4H_002575"
     CDS             complement(3284..4144)
                     /locus_tag="ACGE4H_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8365949.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAXWLB010000188         4236 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXWLB010000188 DAXWLB010000000
VERSION     DAXWLB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.191"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            229..1200
                     /locus_tag="ACGE4H_002576"
     CDS             229..1200
                     /locus_tag="ACGE4H_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8365950.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1232..2629
                     /locus_tag="ACGE4H_002577"
     CDS             1232..2629
                     /locus_tag="ACGE4H_002577"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFF8365951.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            2788..3411
                     /locus_tag="ACGE4H_002578"
     CDS             2788..3411
                     /locus_tag="ACGE4H_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365952.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1495 a          682 c          909 g         1150 t
ORIGIN      
        1 tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat taaaaatgaa
       61 ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc tactgaagac
      121 tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt catcataatc
      181 gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat ggcatcgatc
      241 cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta cttgaatggt
      301 cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc gttggactat
      361 aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat tggacttttg
      421 ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga aagagtagct
      481 gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga aaaaattgaa
      541 caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt cttatccgaa
      601 gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat cgcattgaat
      661 tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga aagtgtagca
      721 aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat tgctgccact
      781 caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt ccaaaatgat
      841 ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg ctattacggg
      901 gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta caatatgtcg
      961 attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga tattgcgttc
     1021 agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat cacgatcacg
     1081 ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg aatcaaaaaa
     1141 gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg aatcaactaa
     1201 aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc cagcaaattt
     1261 ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag gcgatggaaa
     1321 aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct ttgatcaagt
     1381 aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac agttgatgaa
     1441 acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga tacctgatga
     1501 atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg atgagttgat
     1561 tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga tgtatctgca
     1621 agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat atgccaaaag
     1681 ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg aaccaatcgt
     1741 tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag atatgaaaaa
     1801 agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca aatcttacca
     1861 tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa gctatccaag
     1921 agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt tcttcaatcg
     1981 ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg agatagtaaa
     2041 agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca aagaaaatac
     2101 agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa aagaaacacc
     2161 aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg aaatgcctgg
     2221 tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct atgatatttt
     2281 gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa acggtatggg
     2341 tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg attatcggat
     2401 cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag gtgccaattg
     2461 tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg catataagaa
     2521 tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa tcaaaaaatc
     2581 gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag aaagaatagt
     2641 tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa gttcaattat
     2701 ttacgatctt tttcaacttt tttgtctttc tatttcattt tttgtatata atagaatagt
     2761 caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct tctcttttgg
     2821 ttattcggtt ttttcttact gcttatttta tccgcagcat tttcaggaaa aatgacagca
     2881 gatcttgctc agaaagaaac agaaaaaatc gtagcaagca aagaatcagt agataatttc
     2941 aaaaaagaat tctcaactta ttggtgggcg gagtataaaa taactgccaa ttacgaaaaa
     3001 ggcgaattgg acatttattt gccaaaagga aaagaagaaa atcaatttat taatatcgat
     3061 gacgtactta gtgcgatttc tctcagacaa tatcaattta aagacaagca tttgaccgtt
     3121 ttgcttttga ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa agtgatcacc
     3181 gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga atggaaaaag
     3241 acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc gacaggaaca
     3301 agtccttcag attctggcag tggacaaacg ataacgggaa gtgaagaaaa tcaagcctca
     3361 attattacga atgagtcatc acaaggacaa tatcaatcct atggacaatg aaaaacagag
     3421 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca
     3481 tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg tgtatcaaga
     3541 ggtcttgagc ctgccgttta actctgagcc acaaggagca atttcaaaaa atagctcctt
     3601 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca
     3661 agaggttgta aaattagcgt cctgacataa tctaataccg agggtcagct gattgaacac
     3721 cgtgtatcaa gaggtcttga gcctgccgtt taactctgag ccacaaggaa caatttcaaa
     3781 aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg gcaggagaac
     3841 accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag gagctgcgac
     3901 aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct tttgaatacc
     3961 gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc gcttacatcg
     4021 cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata aaaaaatctt
     4081 aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga aaaaatgtga
     4141 atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc gtttagcaat
     4201 cgttttgaca gctgttttct ttttctggct gaagac
//
LOCUS       DAXWLB010000189         4213 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXWLB010000189 DAXWLB010000000
VERSION     DAXWLB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.192"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            478..1446
                     /locus_tag="ACGE4H_002579"
     CDS             478..1446
                     /locus_tag="ACGE4H_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8365953.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1514..2272
                     /locus_tag="ACGE4H_002580"
     CDS             1514..2272
                     /locus_tag="ACGE4H_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFF8365954.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2340..3050
                     /locus_tag="ACGE4H_002581"
     CDS             2340..3050
                     /locus_tag="ACGE4H_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFF8365955.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3086..>4213
                     /locus_tag="ACGE4H_002582"
     CDS             3086..>4213
                     /locus_tag="ACGE4H_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8365956.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1411 a          631 c          892 g         1279 t
ORIGIN      
        1 ataaattgta ttaaaatcaa tgtatcactt tttaatcagt tttaagacca taaattgcgt
       61 actaaaagaa attctgcaat tcaaaataaa gcattttaag ccattttcaa atttaagtgc
      121 acccatctac ccgtcaaatc actttatttt ccactccagt agtttcctaa ttcaactgtg
      181 tgcaaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca tcccagcacc
      241 ataattattt aaatcatagg catttttcga aattagtata tgtactcaag tttcactgca
      301 gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa agaaagcgga
      361 tacaatgcaa ttatgtcaca tgacgttata tgacgttatg tgatatcttt acatttaatc
      421 agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga aatgttgatg
      481 gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat ttctggaatt
      541 tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta ttttcttaaa
      601 gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa ccatgtaacc
      661 ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg aaatacagca
      721 gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt tggtctgact
      781 cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta cgaatgggga
      841 gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac tgtatctttg
      901 gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat gaaggttatc
      961 gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt gtttgctgat
     1021 aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata tggacatgct
     1081 tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac ttctgttcat
     1141 tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa ttttattctc
     1201 ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt ccttgataaa
     1261 tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat gatatcagat
     1321 gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga atatcgttca
     1381 gctttagcag atgtacgcaa tcatgattta gatgttcgtc gctatatggg gaaagtggag
     1441 tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg ataaatatga
     1501 aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt ttgcaaaaaa
     1561 actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg cagctcatgt
     1621 tttatcagca ataaaagaaa taggactgga caacagtcac tctcgctttg aaaaaggcga
     1681 gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg gcagcaattc
     1741 gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag aatatttaaa
     1801 ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat tagaagagtt
     1861 acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg aagaagaggt
     1921 tcagcgggca attgataaag ttgattttgg cttcttttct atagaagact tttccgatga
     1981 agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat tgattgcaac
     2041 ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag tgaaccccgt
     2101 gctagaagaa ccagttgata caatggcagc gggagattct ttcttaacag ctttcctact
     2161 ttattttctc gaaggggacg atatagtcac cgcgatgaaa aaggggaatg aatttgctgg
     2221 gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt gagcttgttt
     2281 taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag gttaaaaaaa
     2341 tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata ttaaaaaata
     2401 aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca gagctgatag
     2461 aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg gtagaagaag
     2521 ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa gtttttcgta
     2581 aaattgaata tattatgggt ttcagtgaga gttgtaaatc taatggtttt acaccgacta
     2641 gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa aaactcgata
     2701 tcgctcaagg tgatgatgtt ttatatatcc agagaaagcg aatggcggat aacatcccca
     2761 ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg gaggaagagc
     2821 tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc aatcctgggg
     2881 aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg gaagtagctg
     2941 ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac ataccaattc
     3001 atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga tttcctgttt
     3061 ggatttcaag gagtagattt tatttatgaa gcaaatattg cggtcaaaaa tgatttttga
     3121 tggagagcag ccacctttca aaggttatat tgtaatcaat gaagggatca ttgaaaaggt
     3181 ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa ttgcttatga
     3241 tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg gttatttggc
     3301 tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg tagagcgtat
     3361 taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg gctgggatgc
     3421 tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc actttggtcc
     3481 aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg ctaagcagac
     3541 ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa ttaaggaaat
     3601 gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat tactgctttc
     3661 aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc tttctaatat
     3721 acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac ctctacagca
     3781 tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct ttggtggagt
     3841 gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg gtgtttatca
     3901 gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag ttgctgcatt
     3961 caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg gttataaagc
     4021 tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta attcgattag
     4081 tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac aagtagtagc
     4141 agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt acatgtcaac
     4201 tgttataaaa aat
//
LOCUS       DAXWLB010000190         4185 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXWLB010000190 DAXWLB010000000
VERSION     DAXWLB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.193"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            25..1374
                     /locus_tag="ACGE4H_002583"
     CDS             25..1374
                     /locus_tag="ACGE4H_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8365957.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1502..2092)
                     /locus_tag="ACGE4H_002584"
     CDS             complement(1502..2092)
                     /locus_tag="ACGE4H_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFF8365958.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2239..3591
                     /gene="brnQ"
                     /locus_tag="ACGE4H_002585"
     CDS             2239..3591
                     /gene="brnQ"
                     /locus_tag="ACGE4H_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFF8365959.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1206 a          779 c          802 g         1398 t
ORIGIN      
        1 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
       61 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      121 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      181 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      241 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      301 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      361 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      421 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      481 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      541 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      601 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      661 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      721 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      781 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      841 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      901 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
      961 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1021 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1081 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1141 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1201 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1261 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1321 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1381 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1441 aaatttttgt gaaatttcgg cttatttctt ggagttaaaa gcttctgtcc cagcctcttt
     1501 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1561 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1621 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1681 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1741 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1801 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1861 tcgaccagac cactcggtag ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1921 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     1981 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2041 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2101 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccgtcgtttt
     2161 atataggaaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2221 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2281 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2341 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2401 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2461 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2521 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2581 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2641 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2701 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2761 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2821 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2881 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     2941 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3001 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3061 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3121 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3181 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3241 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3301 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3361 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3421 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3481 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3541 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3601 ttgtgaaaga gacgttcagc cttattgccg ataggctgtc tcttgaacac tgtttattga
     3661 gaggtcgtga aaaagccgct cagtttcgag tattaagaaa tattttttga aaatggcttc
     3721 tcacattttt gcataaattt gacttaatac cgagaaactg acttttgaac accgtttatt
     3781 gagaggtcgt gaaaaagccg cttagtttca agtattaaga aatattttgt gaaaatggct
     3841 tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga acaccgttta
     3901 ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt agctccatat
     3961 actttgccgt tttgttttga actaataccg aagagaagct gatcaaacaa catttctttt
     4021 ataatataga ttttagacat atatatgata taaaaatagt tttcgcaaaa aaacttaaca
     4081 actttcgaaa actttcgtca caaaatattc acaactagtg actattatat aagcatacca
     4141 acaaacaacc ctaacaacac tttttcattt ttaactcctt atcct
//
LOCUS       DAXWLB010000191         4075 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXWLB010000191 DAXWLB010000000
VERSION     DAXWLB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.194"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            3..455
                     /locus_tag="ACGE4H_002586"
     CDS             3..455
                     /locus_tag="ACGE4H_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365960.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            469..1158
                     /locus_tag="ACGE4H_002587"
     CDS             469..1158
                     /locus_tag="ACGE4H_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365961.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1283..3634
                     /locus_tag="ACGE4H_002588"
     CDS             1283..3634
                     /locus_tag="ACGE4H_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFF8365962.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3714..4075
                     /locus_tag="ACGE4H_002589"
                     /pseudo
     CDS             3714..4075
                     /locus_tag="ACGE4H_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1415 a          524 c          830 g         1306 t
ORIGIN      
        1 tcgtgaaaaa aacgatacta agtatttttt cagtatttac aatattaatt gggggctatt
       61 ttcttttgaa tttagattct gttaattcaa acgctcaaat aaaagatcaa agagtagctg
      121 aacataataa taaaaaaatg agtttgacca aaaatatcta ttctatgtca gatgaagaat
      181 tgtataaaga atttggtgat ttatccgggg aacagttaga agaaagtatt ccaggaatta
      241 gtgactcaga taaaattgtt gtcttgtctg ctggtgggca attaatggga gatggcaaat
      301 ccgaattagt tagttataaa caaaatcaca gacagtttat tgatgttaat actgattcca
      361 acgctatttc gattaaggat ctaaaagagc ttttaaatag taaaagaaat gaattaaagc
      421 agataaaaga aactaattca gtggaattag agtaaaggaa gattcattat gaaaaagatt
      481 tttggtgttt ctgttgggct acttatgatg ttaacatttg gaaatgcagt atatgctgct
      541 gagaaagaag ttatttcaga tccagagcag tacgcctcac gatactttgc tgattttgaa
      601 gaagaagata aagtacttgc attgccagaa ggtggttttt taaaagggga agctaaaatt
      661 gtatctatgg ataatccaga tataattcaa ggttcatata attcagaaac tgatcctgct
      721 tcagtttcag tgacagaagc aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa
      781 actactccaa ttatgcctcg aggagcattt ccacccgatg ttggtactag cggacacctt
      841 cttgtactag caggaggagg aagctataaa tcacagccat ttagtggtgc gggatggcgt
      901 tttggaggta tgggtgtaaa accagctgat ggtacaggca actttttacg ttggaaatcc
      961 tacattgacg atggaagagt aggaaatgta aatcaagcca acgcaactaa atctggttct
     1021 attcaaggaa tggcattgta tgctcgtgaa ggattcaaat actttgcacc aaacgggcct
     1081 ggttggacaa tgaatatagg tatttattac acttataatc ctgttgcagg tacgtattat
     1141 gttgttcaaa atatttaata aaacagcaca aagagatttc tttgtgctgt tttattattt
     1201 ttagttgttg acaaacacta aaatataaaa tataatcaac acataaacga acgtatgttc
     1261 ggcaagaaag caggggattt ttatggatga gatggtttta agtactcaga aatggctaaa
     1321 caagaaatac agtaatgtta cagggtttga taaggttcca gaaaatgggc gaacagggtg
     1381 gcctacaatc tatggactta ttgaaggact acaagtagaa cttggtatta ctaatctagt
     1441 agctaatttt ggacctacca ctgaaaaaat gtacgataat caggttactc ctaaatgggg
     1501 caaaaatcta cctaaaaata ttgtatttct tattcaaggt gccttttggt gtaaggggat
     1561 taatccaggt ggcttcgatg gggtatatac tccatattta gatactgctg taaaagagtt
     1621 gcagactgat gcagggttta atggaagcgc tgtaactgga gttttggatg cgaaatgggc
     1681 caaagcgtta tttgatatga gtgcttttgt tcttgttccc ggcggagata gtaaaattag
     1741 aaaaatgcag caatggttaa atgtaaatta tttagaatac acgggaataa tgccttgtga
     1801 tggtatttat caaagaaatt caaatcaagc attaattttt gcattacaag ctgaactagg
     1861 atattctccc agtgaagcaa cagggtctta tggtaatggg acaactagta aaacttatcc
     1921 tgtgtctgaa ggtaactcgg gaaactatgt aagaataatt caatatggat tatatgttaa
     1981 tggttatttt gaagacggta cattcgatgg tattttcact aaatatatgg gtttagaagt
     2041 actggcattc cgaaaattta tggtattgcc tgaatatact gaaatagcag atgttgttgt
     2101 aataaaagga ctattgtcta gcgctggaga tattagaaga tctgcagatg gcgcagatac
     2161 ttctacgcaa ttaactagat ctcaaatcca aacattggtt gataatgata taaaaatagt
     2221 tggtagatat cttactggta ctgttggaat tggaaaagat gaacgaaata agtatctaac
     2281 tacagaggaa ttgaataata ttttttctaa aaatttatct gtgtttccta tctatcaaga
     2341 tggaggtgca gcattagctt attttactta tgatcgtggc ttatcagatg gtaagaaggc
     2401 tatagatgca gcaaaaaatc taaatatacc attaacaaca gttatttatt ttgctgttga
     2461 tctagacatg ttaggggaag aaattcttgc atatgctgga gaatatttta agggggtatc
     2521 tgctgtaatg tcatatagcg ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc
     2581 tcaaataatc aataacgggt atgcttcgtt tgcatttgtt tcagatatgt cgactggtta
     2641 tagtgggaat ttaggttttc ctatgcctag acaatggtct tttgatcaat ttgttgaatt
     2701 taccatagga agtggcaacg gagctgttgg tatcgattta atcgctacat cgggtagaga
     2761 tagcggattt aatgagttat caaatgatac taataatgat aattacattg ccaaatacaa
     2821 tcagaaggtt ataaatcaga tggtacgtgc gtatcaatat ctatcacaag ctggtcttga
     2881 taatccttgg aaccctcatt tgacatttta tagatatgta aattattctg gtttgtcatg
     2941 ggacatcatt tcttctcctg taacagagca tgatagaaaa atttatgatg aatataggaa
     3001 taaattaact aatagcgagg gcctgtataa ttattttata gatcctaata ctggttcaat
     3061 aataggtttg cctcacttaa ttgtgacgtt acaatcacag atgtttctag ccgacacaat
     3121 caatgatagt gtttctgact ttgcaggttg gttaggagat ttaatgactt gctggggtga
     3181 agttaagaaa ttaggaatac aaatggagca aggtgtattt gcattagtag gtacggctgc
     3241 aggtggaaca tttagtctag aagatttata ccaagatgtt gacgcggtaa atgtttatac
     3301 cttagcaaaa agaaattctg attcaacagc tttatcggct ttaaaaacgt actattgtga
     3361 taagggtgct attgatagat ttaatcaatt tgttaagaac agatttggtt cttcagataa
     3421 aatcgcttca gtaacaaata gtttattaga aaatgacgaa acagttgata tcttaggtgt
     3481 agggataatt tcatttttca ttgataaaat agttaataat tcacttggga cagattttac
     3541 ttatcttgat tttattggag atgaggggaa agttgcaaat ggattttctg aaaaaattat
     3601 tcaatttcaa gaaaatagaa gtaaacttaa ttaagaatcg tttactattt aaacagtgtc
     3661 cttttccttt ttaaatatat acgataaaat aagtatatga attgtaaaag gacatgatga
     3721 ttatggaaga aatagaatgg aataatctga ctcaaaataa taaaaataat tctggatgtg
     3781 gaattatctt tatgtttgtt atagttttgg gaatagtagt acttttaata actgtcaaga
     3841 tagtagatta ctttgcgaat actcctgaag aacaaataca gagaaaactt cctgagtttg
     3901 aaagagtacc accttatgat ccaagagata attcatttca agtaaaaaaa ttaaaagagt
     3961 ctccaaatga aatgtggttt gaagttacag tttaatcaag cgacttcaaa gaaaaaaaga
     4021 aagaatacaa ttatattgta aaggcaaaag tatcaaaaag atttattttt aaccc
//
LOCUS       DAXWLB010000192         4071 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXWLB010000192 DAXWLB010000000
VERSION     DAXWLB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.195"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..379
                     /locus_tag="ACGE4H_002590"
     CDS             <1..379
                     /locus_tag="ACGE4H_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365963.1"
                     /translation="CSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKG
                     GHGRKRKLDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGE
                     WAYYQLKEQLVNKGKRYGIEVRS"
     gene            976..1371
                     /locus_tag="ACGE4H_002591"
                     /pseudo
     CDS             976..1371
                     /locus_tag="ACGE4H_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            1449..2488
                     /locus_tag="ACGE4H_002592"
     CDS             join(1449..1946,1949..2488)
                     /locus_tag="ACGE4H_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFF8365964.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            2501..3439
                     /locus_tag="ACGE4H_002593"
                     /pseudo
     CDS             2501..3439
                     /locus_tag="ACGE4H_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            3432..3779
                     /locus_tag="ACGE4H_002594"
     CDS             3432..3779
                     /locus_tag="ACGE4H_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365965.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            3776..3958
                     /locus_tag="ACGE4H_002595"
     CDS             3776..3958
                     /locus_tag="ACGE4H_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365966.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
BASE COUNT         1460 a          667 c          823 g         1121 t
ORIGIN      
        1 gtgtagcggt gaatgtttaa ttaatgataa atctgtaatg tcttttggtg atagccaata
       61 tataagaaac ttcaaaagta aaattttagc aatgaaatca aaagaacaaa gccgaggagt
      121 cttttctaag ggcggtcatg gtcgaaaaag aaaattagat caagatcgat gggaagcaat
      181 aaagaaaaga gaaagtaatt ttgttaaaac ttataataat caatgttctg caaaaatcgt
      241 taaaactgct attcgcaata aagctcaaat tattcgtatc attgaaccag ataaggctta
      301 tggcgaatgg gcttactatc aattgaaaga acaactagta aacaaaggaa aaagatatgg
      361 gatagaagtc agaagttagt aaattaaaca atcgttgatt tggcaacgaa atagtgaggg
      421 aaaatccact cactgaaggg taaactccgt caagttgtgt gaccagacgg aaggacgaat
      481 gttgttgagg ttttagaacc atataaaaca gcatgtttct caaattttta ggataaggga
      541 aattgctagc agtttgtttt agaaccatat aaaacagcat gtttctcaaa tgagtacttt
      601 aaattaattg atggcgttcc tgctttagaa ccatataaaa caacatggat ttaaaacaag
      661 aagtaagtca gttctccttt tactttagta aggaaaaata aagtattctc aataaaactt
      721 aaaataagca aacctttgtt ttaaaatgat tgtcaagtta aaaaaatctg ggatcatata
      781 aaatagtata gttctcaaat atgattagaa accctattaa agagcaatat taagccaact
      841 tacttaaatc gtttaattaa gaacaataat ctcttttagt cagactttta gtctgacttt
      901 tttgtatttc caaaatttat tctttacaaa aacgaacata cgtttgtata attacttgtg
      961 aatgggggaa taaaaatgtt tgattacagt agagaaccgg tgaatgacta ttttttaatc
     1021 gacatgaagt ctttctatgc tagcgtggaa tgtattgaac gaaatttaga cccaatgact
     1081 acaccgttaa ttgtcatgag tagatcggac aacactggtt caggtctgac tctagcttct
     1141 tcacctactg caaagaaaaa atatcatatt actaatgtta gtagagctac tgatttacct
     1201 tatccattac caaaagaact tcatattgtt ccaccacgaa tgaacttata tataaaaaaa
     1261 aatatggaag ttaatcagat ttttagaaag tatgtcgcag atgaagattt attgatttat
     1321 tcgattgatg aatcaatttt aaaagtgaca aaatccttaa atttatttac tatataaatt
     1381 gagttaaatt gtattcgttt ttcctcctat aggcgtatac taaagaaaac acttgaggag
     1441 tggggaacat ggaaacagaa cgccaaatcc gtgaattgga aaaagcgatg aatacgacag
     1501 acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt ggttcttgaa ggccacaccc
     1561 ggaaagaagc cgggcacacc atcggccgcg acgagcatac tgtcagccac tatgtgaaaa
     1621 actactgcca taatggaatc caaggtttag tctccaaaaa acaaagcgga cgaccttcac
     1681 ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt cgcctaccac actccggagg
     1741 aagtaggttt caaatcccgt gccaattgga cattggcgat tgtgtgtgac ctgattctcc
     1801 gggaatggag ttatggatat acgcaaaaag gcgtggcgga cctccttcac cggctaggac
     1861 taagctggac ccgtccgacg tataccctca aaaaagcgga ttccaagaaa caacaggtat
     1921 ttttggaaga aaccttcccg agcctaaaaa aaaattgctg aatagcgata ttgaccacct
     1981 cctattcgag gacgaaacca tgatccggga ttaccaagcc attggctgca cctggtttct
     2041 ccgcggccac cagcgaaaaa tccccactta tggacaacac aaaggggtaa aattgatagg
     2101 caccttggat tatgaaaccg gtcggatcat ttgtacagaa gaaggggcat acgacgcagc
     2161 ggccttcctc cgttttctgg agcggacgtt gcgggaatac cccaccggaa aaatcgtgat
     2221 ggtattggat aatgccctta tccaccacgc caagctgatc cagtcttttc ttgaagaaaa
     2281 cgctaggcgc ctggcactca tcttcctccc tccctacagc cctcagttga acctaatgga
     2341 aggcgtttgg aagtggctca aggaatcggt catcaacaat gttttttttg accacgtcca
     2401 gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta aacgagcgtc cccttgaggt
     2461 gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc aacctatata gttcaggaac
     2521 aagaagcgag agaagaagag aattagctaa aataatcaaa gatgagattc agaaagaaac
     2581 aggtctaata gcaacagtcg gaataggaga taacccatta ttagctaaac ttgcactgga
     2641 caatgaagcg aagagtagta aagaatttat agctgagtgg acttatgaga ctgtacctga
     2701 aaaaatatgg aaaataaacg agatgactga cttttggggc attggatcac gcatgaaaaa
     2761 aagattgaat catatgggaa ttatgagtat agaagattta gcaaattgga atccctacac
     2821 aattaaaagt cagttaggtg taatcggact ccagttatat ttccatacca atggaattga
     2881 tcgtacggat attgctattc caccagaacc aaccaaagaa aagtcttatg ggaattcaca
     2941 agttctacca aaagattata cgaggagaga agaagtagaa ctagtagtca aggagatggc
     3001 agagcaagta gccattcgaa ttagacaaca caattgcaaa actggttgtg tacgcttagc
     3061 aattggtacg tcaattttgg aagaaaaatc aggtttctcg caccagatga aaataccaat
     3121 tacagataat acgaaagaat tacaaaatta ttgtcttttt ttattcaata agttttatga
     3181 ggggcaggaa gtacgacata taggagtaac ttatagcaga ttagtataca cagattccct
     3241 acaattagat ttatttagtg atccaagaaa aaagattgct gaagaaaatt tggataggat
     3301 tattgataag attagacaaa aatatggatt tacagctatt atacatgctt catctatgtt
     3361 agaaggtgca agaagtatta cgagaagcac tttaatagga ggacacgcag gaggattagg
     3421 aggtttgcat aatgattgaa tataacgata gaggacgttt aaaatggtcg ggattttatt
     3481 tgtctgatca tactgaaaga atcaatgcag acaatgtaca acgtgctaat cataatttgg
     3541 caaaagaaca aatgacgact gaagagattg cgaaaatact gagaactgct gtcataaaaa
     3601 atagacccgt ctgcattcaa aaagaggaac gaaacgctga aggttactat ccagatgatg
     3661 tagtaggaaa aattattgga tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc
     3721 actatgatga aattaggaat gtttctttct cggaattatt aaaatggagt cagatatgaa
     3781 cattattaaa aaatacgaat tcggttatat tacgtatgaa cagctaatag aggaattatg
     3841 gggatatggt caacaattaa ttaatgaagt ggggattgat tgcttttgtt tctatattga
     3901 atccggagca ggataccata agaacagata ctatatattt ccatatccac caaaatagtc
     3961 tcttagatat ttttaaattt tctccaaata ctttcaatta ttgtgaaata tttcccttta
     4021 attaatcaaa ttttttataa aagtaatata taaaataaat tgcttgaagt a
//
LOCUS       DAXWLB010000193         4044 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXWLB010000193 DAXWLB010000000
VERSION     DAXWLB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.196"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(186..1112)
                     /gene="glsA"
                     /locus_tag="ACGE4H_002596"
     CDS             complement(186..1112)
                     /gene="glsA"
                     /locus_tag="ACGE4H_002596"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFF8365967.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1135..2562)
                     /locus_tag="ACGE4H_002597"
     CDS             complement(1135..2562)
                     /locus_tag="ACGE4H_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFF8365968.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2593..3807)
                     /locus_tag="ACGE4H_002598"
     CDS             complement(2593..3807)
                     /locus_tag="ACGE4H_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFF8365969.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1272 a          862 c          723 g         1187 t
ORIGIN      
        1 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
       61 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      121 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      181 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      241 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      301 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      361 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      421 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      481 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      541 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      601 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
      661 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
      721 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
      781 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
      841 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
      901 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
      961 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1021 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1081 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1141 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1201 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1261 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1321 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1381 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1441 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1501 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1561 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1621 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     1681 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     1741 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     1801 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     1861 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     1921 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     1981 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2041 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2101 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2161 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2221 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2281 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2341 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2401 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2461 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2521 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2581 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     2641 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     2701 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     2761 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     2821 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     2881 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     2941 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3001 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3061 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3121 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3181 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3241 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3301 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3361 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3421 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3481 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3541 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3601 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     3661 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     3721 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     3781 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     3841 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     3901 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     3961 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     4021 ttaaagatat tttttcataa ggaa
//
LOCUS       DAXWLB010000194         3886 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXWLB010000194 DAXWLB010000000
VERSION     DAXWLB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.197"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..912
                     /locus_tag="ACGE4H_002599"
     CDS             <1..912
                     /locus_tag="ACGE4H_002599"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFF8365970.1"
                     /translation="AINELEKRNLEIHELNQKNNSILGMKIRNKINQLNQMKKRDNLL
                     PAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIKEP
                     DFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYDFT
                     LYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIEQI
                     SKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVLWK
                     SGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1164..2174
                     /locus_tag="ACGE4H_002600"
     CDS             1164..2174
                     /locus_tag="ACGE4H_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFF8365971.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2542..>3886
                     /locus_tag="ACGE4H_002601"
     CDS             2542..>3886
                     /locus_tag="ACGE4H_002601"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8365972.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFS"
BASE COUNT         1320 a          520 c          695 g         1351 t
ORIGIN      
        1 gctatcaatg aattggaaaa aagaaatctt gaaatacatg aattaaatca aaaaaataat
       61 tctattttag gcatgaaaat acgaaataaa attaatcaac ttaaccaaat gaaaaaacga
      121 gataatctgt tgccagcaaa atataccctt aaatcgatga ccggtaaggg gcctattaaa
      181 gactattcaa acagagagaa tcgcagtgat gaactaagaa aaaaagagtt aactaatcaa
      241 ggaacaccta aaaggattgc tgtgtatagt tgtatcactg gtaattatga ttctattaaa
      301 gaaccggatt tttctcatcc attggtagat tactatttgt atacagataa tgtgattaca
      361 agtgagcatt ggaatattag agagatacct aaatatatat cggaattaaa aaataatatt
      421 ttgatcaata gatatattaa atttcatcct aatgagctat ttactgacta cgattttact
      481 ttgtatgttg atggtaatgt aacaataact tctgatatta ccggattact gaacagttct
      541 tccaaacttg ggttaagtat gcataaacat agtgaaagaa aatctttata ttctgaggca
      601 gatgcattaa ttgcagttgg caaaggtaat aaggaaagta ttatagagca aattagtaag
      661 tacagagaag aaggatttcc tgataatttt ggattggctg aagccacggt tattttagtt
      721 gatttaaata attcaatcgc tgagaatctt ctagatttgt ggtggagaga gtatttgaac
      781 tcagaaagtg ggcgagatca gctcgcattt ccttacgtgt tgtggaagtc cggacatgca
      841 atgaaggaag tgactacctt aggcaataat gtatataaaa gcgctaaatt aagaatcata
      901 agtcattatt agccctaaat gatttctaaa ctattctttc aaatcctagt aaatatttgg
      961 agatagaaga tttccttttt aaagtcataa aaaattaagt tatatttttt gagtgaatgg
     1021 agaagacgca tagtatcata aacttcgtta tatcttaaat tatggggtat gcctaaataa
     1081 taaatgtaat acgagtataa agaagaaagc aattttgggt ttaaccctat tggttccttt
     1141 cagaaaaagg tggatgaata aaaatgaaaa tattaataac aggtggagca ggatttattg
     1201 gttttcattt gtctagaaaa ttactcaagg aaactgaaaa tcaaattgta gcgattgata
     1261 atctcaatga ttattatgat gtccaattaa aagaagatcg attaacgata ctgtacgatt
     1321 ataataattt taaatttatc agaatggatc tattagagaa agaaaaatta gattctctat
     1381 ttgaaagtga aggttttgac atagtaattc atttagctgc tcaagcaggt gttagatatt
     1441 ctattgaaaa cccttatgct tatattgatt ctaatattat cggttttctt aatattcttg
     1501 aagcctgccg aaacttccct gtaaagcatc tattatatgc atcatctagt tcagtatatg
     1561 gtgggaacac gaaagtaccg ttttcaacag aagattcggt ggatcatcca gttagccttt
     1621 atgcggctac caaaaaatcg aatgaattaa tggctcatac ttatagccac ctttataaaa
     1681 taccaacaac tgggctgaga tttttcactg tctatggtcc atttgggcga ccagatatgg
     1741 cctatttctc ttttaccaag aatattttat ctggaaaaga aattaagatt tttaaccatg
     1801 gaaagatgga aagagatttc acttacatcg atgatatcgt agagggaatc actagattaa
     1861 tcaataaagt acctgaaccc aactccgact ggacagacat cgatgatttg agtagtgaaa
     1921 gttgggcacc atataagata tacaatattg gaaataataa gccggttgca ttgttggagt
     1981 ttgttgaaac aattgaaaag tatttaggtg ttaaagcgaa caagattttt ttggacatgc
     2041 agccgggtga tgtcgttaga acttatgccg atattgaaga tttagaggaa aaaatcaact
     2101 ttaaaccgaa tataaccttg gatgagggca ttaaaaaatt cgttgaatgg tatacagtgt
     2161 attatttgag ttaattttga aaggaatatt gaagatgggt aattcatacg atttttttag
     2221 ctttgttagg tattttagga aattctactt tcaaacgtga cgaactttct cgccgaatag
     2281 atattttcta taactaaaag aatttctata aaacaaaatt gtgtatcaag atgtgtgaac
     2341 tggattctga ctgccaatat tgattgatga gattgaagaa atatatagtt gatcatggat
     2401 atctttattt gaaaagatct tagctattca catattcaaa aaaattttta ctacgaggac
     2461 tcacacgata cagaggaatt taggtagttc taattaaacc agaaagtcat ctatagatgt
     2521 gagttgaggg aggtcatacg gttggatagt agcagtaata aatcacattc aatagctaaa
     2581 aaagctttta tttacgcaat aggaacatta tcatcaaaaa tgatatctgt cattataatc
     2641 cctatctatg cagtatatat ttctgttgcc gatttaggaa gctttgatta tcaaaataca
     2701 attgctcaat ttctttctcc agttattttt ggagcaatat gggaagcaat tttacgcatg
     2761 ggattaggca aagaaaaaaa tattcagaaa aaagtagtga cgaacagtat ttgttttgtt
     2821 atagcaaata cagtgatatt agccattttt ttaccaataa tatatagaat gattttcccg
     2881 aatttagagt attatcaatt gtttgttttt caattaattt tcactcctat cctaagtgtt
     2941 tttcaatata ttgtaagagc actaggtgaa tcaaagattt ttgtgatgag tggtataatc
     3001 gctgcgggta tcaacctgtt actccttact atttttgttg ttacaatggg tatggggata
     3061 cttggtctct tactctctat cataatttct aatgttataa atttgatttt tttgattttc
     3121 agggggcaga tctttcaata tttcgcaacg agttcttttg attttttatt tttaaagtcc
     3181 atgctgaaat actctatccc actcactttg aatctcactt ttggatggct aatagttggt
     3241 ttttcaagat tttatattgg aacgtacatc ggagtaacag aaaatggtat tttttctttt
     3301 gcgatgaaat tttcatctat aattttaact cttacaagta ttgttagtat gtctatgata
     3361 gaggatgcgg ttatcacgtc tgagaatgat gatttcatcg aaagattcgt tgagaaaaat
     3421 acctatattt ttaaaatgta cattaggtta gcgactcttt ctattcctat catttgctgt
     3481 gcatactctt ttataaccaa ccctgattat aaaaaatcat tagttctagt tccaatatta
     3541 gttattatgg catgtttcag tgggatgaca acaaatatcg gaaatatatt tgcagtatac
     3601 aataaaacaa atcagttatt aatgatttca gtagtgtcag gtctattgag tgttgttggc
     3661 acctatttat taggctattc ttttggagta attggtgtct catttgctac tttgctcggt
     3721 actttagtat tatttggact tagatattat tttggaagga aaattgctta ttatgcattt
     3781 gattggaagc gaatatcgct acttttagtt ctctattccg caatcgcttt tgtatgcatt
     3841 catagaggct tctattttaa tcttttctta acgattattt ttagtg
//
LOCUS       DAXWLB010000195         3862 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXWLB010000195 DAXWLB010000000
VERSION     DAXWLB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.198"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..665
                     /locus_tag="ACGE4H_002602"
     CDS             <1..665
                     /locus_tag="ACGE4H_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365973.1"
                     /translation="AYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNL
                     TMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGF
                     ILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVT
                     VKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKV
                     VI"
     gene            709..978
                     /locus_tag="ACGE4H_002603"
     CDS             709..978
                     /locus_tag="ACGE4H_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365974.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1210..1506
                     /locus_tag="ACGE4H_002604"
     CDS             1210..1506
                     /locus_tag="ACGE4H_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365975.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1508..1930
                     /locus_tag="ACGE4H_002605"
     CDS             1508..1930
                     /locus_tag="ACGE4H_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365976.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1949..3541
                     /gene="mobP2"
                     /locus_tag="ACGE4H_002606"
     CDS             1949..3541
                     /gene="mobP2"
                     /locus_tag="ACGE4H_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8365977.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3562..>3862
                     /locus_tag="ACGE4H_002607"
     CDS             3562..>3862
                     /locus_tag="ACGE4H_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365978.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCK"
BASE COUNT         1517 a          553 c          756 g         1036 t
ORIGIN      
        1 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
       61 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
      121 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
      181 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
      241 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
      301 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
      361 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
      421 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
      481 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccaaa
      541 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
      601 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
      661 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
      721 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
      781 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
      841 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
      901 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
      961 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
     1021 cgtgacccca cttttaaaaa aagtggttgg ataggctgtg cctatcacgt ttctgaaaaa
     1081 aataaatgac cccaaaatac caatatatag gatagtacaa ggatagcaaa ggatagtaaa
     1141 aggatagagg taggatagcg aaaaggatag gatccttttc actatcctat attatattgg
     1201 aggatattta tgaatgttaa aattagagat ttagatccaa aattcatttc tgaaattgat
     1261 cgaagatgcg ttgaactaag taatcgtaca ggaaataaat ggagtcgtaa tgattattta
     1321 aagctactcg ttgaaaatga ttttgatcgt cctttgatgg aatacaaaca agaaaagttt
     1381 gaccagttat tagaaaagtt ttcagaaatt caaagttata acacaaagat attagaagaa
     1441 tatgttagcc aaaataatcg tattatagaa atcttgattg agcaaaatag aggaagtgaa
     1501 ctttgatatg gcaaaggtta tagacaaaca catttacttt caccaaacac atatcgatgt
     1561 attggatgaa actatttcta ctcatgcaga ggttaaaaac tatagtgaag ctgttcgctt
     1621 tttatgtatg aactatgatc cagaagtgga gaaaaaagta aattccaatc aagcaaagtt
     1681 gaatggaatg agtaaagaaa tttctatttt aaccgaaatg gtttcagaat ttgcggatct
     1741 tcatttgaaa gatacaggaa tattaattgg taaaaattca actgtttatc aagaagcaaa
     1801 agaacgagtg gaaggaaaaa ttaaatccaa tcaaacaaaa aaatattccc ctcagttgaa
     1861 gaaacctgtg aatgaatctg ttcatgccag agtagatcca tttgatccaa cgaagattct
     1921 aaaaagataa aaatgaagga ggataataat gactcaatcg ccttcagtta ttctcacttg
     1981 tcaatttact ttgccgaata aaaagtcatt ttctacttat attgactata ttacaaggga
     2041 acaggctctt gaagaaaagg aacgacgtac gcccgaagaa gaacaggaac ttcaacgaat
     2101 caagaatgct ttagaggagt tttatattcc tgaaggagaa actttttcgg agagtaagac
     2161 gggtaaagaa ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa
     2221 ttttgacaag ttagaggaac aagattttac caattatctt tcttatatga cacgtcacta
     2281 tgcgttagaa caaaagaaag aactgacgca aagtgaacaa aaagaatacc aacgcttgaa
     2341 ccaagcaatg aaaaaatatc aaacagaacc ccataaaaaa gcttcaggaa atcttcctgg
     2401 agttttttct atagcttcag acgaagtgaa aggaagtgat ctgaaggaga ttaagcaaat
     2461 cttccaaaga ggacaacaaa aaggctcaat catttatcaa gatgttgttt ctcatgataa
     2521 ttcgtatctt gaaaaaatag gattgtacga tcccaaaaca gatactttag atgaagaagg
     2581 gttaaaagca gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga
     2641 aacaggttac tggatggcaa cgattcaccg taacactaaa catatccata ttcattttgc
     2701 agttgttgaa aaagaaaata cgcgtaaacc ttacacggag gttgaaaatg gtgtgagtta
     2761 tatcgtaccg aaaggtaaga gaagacaagc aacattagac gcaatgaaga ccaattatgt
     2821 acatgagtta gaacgttttt cttatgaaaa ggatcgaatg attggtatgg agaaacgaaa
     2881 tttattgacg cggaaaagtg atttgcgtaa caccttaaca aaagtggtaa aagagccagc
     2941 gcgttacgat cgacacgcga tgaacttatt aaatgaagta tataaaaccc tacctgacaa
     3001 acgttcggaa tggaattacg gggatgaaaa acgaaccaaa ctttcaccat acacaagaga
     3061 gaaactcgat gagttaacca aatatgtttt aacaaaagaa cctgaatatc aagaatatat
     3121 acagatttca aaagcattga aagaagaagc aaaaagtctt tatggggaaa gcaagagaga
     3181 atcaaaggat gcagagaaaa atgctctctt tgatttaaaa aagcgcacag gaaatgccat
     3241 cctaacagag ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgttgatca
     3301 gatgcaatct atttacaatg aacgatcctt tcgaacgctt aaacaattgc caacgaatga
     3361 atacaaatat agttacgaag aattcatgaa gcgcaggaaa caattccaac aaagagttgt
     3421 cacgcagcgg agtttgcgtc aattaaatcg aacgattaat gcccatgaag aaaaatacca
     3481 cgcaagtaaa gcatacgaag agcaacaacg aagaattcaa atggaacaag aaagacagta
     3541 aagacagtag gaggaatagc aatgaaacaa tatttagatc aatggaaagt tatagaagga
     3601 tcattaagag aagaacgaat cgaacaactt cctgattgtt tagaaaaaga gcatttgttc
     3661 cagataagag aaatgttgag aaacgaacag tttgatccaa atcaattttt agtcgttgag
     3721 tacccagcta ctggtgtgta ttgttgtaat catgtgaatg gtgaaaaata ttttatcatt
     3781 caagaatatg agggcaagtt agcaccttat tatactactt gggaaatgaa tgaagaggga
     3841 atcaataact ttccatgtaa aa
//
LOCUS       DAXWLB010000196         3776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXWLB010000196 DAXWLB010000000
VERSION     DAXWLB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.199"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4H_002608"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACGE4H_002608"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFF8365979.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACGE4H_002609"
     CDS             complement(1172..1867)
                     /locus_tag="ACGE4H_002609"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFF8365980.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACGE4H_002610"
     CDS             complement(1880..2596)
                     /locus_tag="ACGE4H_002610"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFF8365981.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="ACGE4H_002611"
     CDS             2926..3531
                     /locus_tag="ACGE4H_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFF8365982.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1078 a          774 c          669 g         1255 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctc
//
LOCUS       DAXWLB010000197         3763 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXWLB010000197 DAXWLB010000000
VERSION     DAXWLB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.200"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..523
                     /locus_tag="ACGE4H_002612"
     CDS             <1..523
                     /locus_tag="ACGE4H_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365983.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2053
                     /locus_tag="ACGE4H_002613"
     CDS             584..2053
                     /locus_tag="ACGE4H_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8365984.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2064..>3763
                     /gene="topB"
                     /locus_tag="ACGE4H_002614"
     CDS             2064..>3763
                     /gene="topB"
                     /locus_tag="ACGE4H_002614"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8365985.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1392 a          638 c          832 g          901 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagctgcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gaacaaaaga atattgttcc
      241 tgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatcca cagcataatc aagtaatttt
      361 aaaaggaaaa agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaattca ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 gacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgct gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg
     1081 tttagatcat gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc
     1141 aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca
     1201 cattgacaag aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca
     1261 tttaaaattc tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa
     1321 actacaagat gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta
     1381 cgttgaagag tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat
     1441 atgtgtggac aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat
     1501 cgttgatcct tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca
     1561 agtacctaaa gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc
     1621 tgaagcaatt atggccatac acaaaactga aactaattta cagctgacaa atcaattggt
     1681 gtctgctcat gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga
     1741 aacaattgat ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg
     1801 cgcagatgga acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt
     1861 taatgcagga atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca
     1921 tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat
     1981 ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca
     2041 agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag
     2101 cgaaggctta tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc
     2161 aagatcgatt gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt
     2221 tagattcgcc cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta
     2281 ttttccccaa tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg
     2341 tcaaacgaca gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag
     2401 gggagctgat tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta
     2461 aaagactttg gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac
     2521 gagacgcaga agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt
     2581 ggttaattgg gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac
     2641 caggaagttt ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc
     2701 aggaaaagat tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg
     2761 ctcaaaatgg cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag
     2821 catttgaagc ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc
     2881 ttcaagtcga aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc
     2941 ttcagtctaa aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc
     3001 aagggttgta tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg
     3061 aaggggaata tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg
     3121 aagcgattcc gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc
     3181 aggaacatta cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac
     3241 taagtccact gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg
     3301 cggaaaagta tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa
     3361 aggcaatcga aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg
     3421 acggaaaaga gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga
     3481 cggtggatct acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta
     3541 cgttgatcac tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca
     3601 tattgaaaga agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct
     3661 tgaaacaaaa ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga
     3721 ttttgctttg ccaagccgtg gaaaaagagc cgctgcttac gag
//
LOCUS       DAXWLB010000198         3754 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXWLB010000198 DAXWLB010000000
VERSION     DAXWLB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.201"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..523
                     /locus_tag="ACGE4H_002615"
     CDS             <1..523
                     /locus_tag="ACGE4H_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8365986.1"
                     /translation="EKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTIS
                     NEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTL
                     HLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNE
                     IYYEQQLSQKERV"
     gene            584..2044
                     /locus_tag="ACGE4H_002616"
     CDS             584..2044
                     /locus_tag="ACGE4H_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFF8365987.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2055..>3754
                     /gene="topB"
                     /locus_tag="ACGE4H_002617"
     CDS             2055..>3754
                     /gene="topB"
                     /locus_tag="ACGE4H_002617"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8365988.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     "
BASE COUNT         1382 a          648 c          825 g          899 t
ORIGIN      
        1 ggagaaagct attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa
       61 ggaagcggcg gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat
      121 tcaaaccatt tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc
      181 agaagtgaag caggagcata aaaatactgt agaaacggca gagaataaga atattgtgcc
      241 cgttggttca ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg
      301 aacgttacat ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt
      361 aaaaggaaaa agttatggaa aagagattca aacggcgtta ccttcagaag aatttgcgct
      421 aggaaatcca ttccgatttt cacttgaaac agtgtcagaa gtggccaaac gaaaaaatga
      481 aatctattat gaacaacaat tgtcacaaaa agaacgcgta taaaacaagt aaaaaagact
      541 atctgaaaaa cggatagtct tttttctttt gggaggtttg aaaatgaccg aaaaaaaaat
      601 gagtcttatt gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat
      661 ggctgtagtc aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga
      721 tcgaagaaaa caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt
      781 agctaaactt tttttcatag acaaagagat ccaagactct aagcaacaat ttaaagcagc
      841 cactgatttt attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga
      901 aacagaaaag tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa
      961 ttatctgaag gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg
     1021 gttgattgcc gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg
     1081 tttagatcat gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc
     1141 gagttatcaa ggaacgttca ttgattatga acgttttggt gagagaggca catataagca
     1201 tatagataaa aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca
     1261 gttaaaattt tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca
     1321 gttaaacgat acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta
     1381 ttttggtgag gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat
     1441 ttgtgtggat aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc
     1501 agtcgatcca tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca
     1561 agtgccaaaa gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc
     1621 agaagctatt atggccatac acaagacaga aaacaatctg caattaacga accaactggt
     1681 ttccgctcac aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga
     1741 agccattaat ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg
     1801 cgtggatggt acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca
     1861 gattcttttc tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga
     1921 atttatacct gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca
     1981 agaaatcaga aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga
     2041 gtgaaatcgt catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt
     2101 atgccgatag cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat
     2161 tgtttcctgg tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc
     2221 ccgatatgta cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta
     2281 cacattatca ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc
     2341 aacttcaatc ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga
     2401 tcgcttggac gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt
     2461 ggatcaactc cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa
     2521 aagagaccta tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg
     2581 ggatgaacgg tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct
     2641 tttccttagg cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga
     2701 ttagaaattt caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg
     2761 gcgcatttga tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag
     2821 attatttaaa agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg
     2881 aaaccgagaa aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta
     2941 aaatgaatca gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt
     3001 atgaagggaa atatctaagc tatccacgaa cagacacccc ttatattacg gaaggggagt
     3061 atgcgtattt gttggatcat ttagatgaat acaagcattt tttaaaggcc gaatcaatcc
     3121 caacccctat tcatacgccg aatagtcggt atgtgaataa caagaaagtc caggaacatt
     3181 acgcgatcat tcctacaaaa acggttatga cagcagcggc ttttgaacaa ctaagtccac
     3241 tgcagcaggc catttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaaat
     3301 atacctatga agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg
     3361 gaaaagtacc gatagatcta ggttggaaga agttatttgg caaagagaac gacggaaaag
     3421 agaaggaaga agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc
     3481 tacaagtcct tgaaaaagaa accaagccac cacagcctta taccgaaggt acgttgatca
     3541 cggcgatgaa aacagctggg aaaaccgttg atagtgaaga agcgcaatcc atattgaaag
     3601 aagtggaagg aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa
     3661 aggaatatat caaggtagag aaaaataagc tcgtggtgac gaataaaggg attttgcttt
     3721 gccaagccgt ggaaaaagag ccgctgctta cgag
//
LOCUS       DAXWLB010000199         3713 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXWLB010000199 DAXWLB010000000
VERSION     DAXWLB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.202"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE4H_002618"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACGE4H_002618"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFF8365989.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACGE4H_002619"
     CDS             complement(2261..2512)
                     /locus_tag="ACGE4H_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFF8365990.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ACGE4H_002620"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ACGE4H_002620"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFF8365991.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ACGE4H_002621"
     CDS             3462..3680
                     /locus_tag="ACGE4H_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365992.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DAXWLB010000200         3677 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXWLB010000200 DAXWLB010000000
VERSION     DAXWLB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.203"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(99..497)
                     /locus_tag="ACGE4H_002622"
     CDS             complement(99..497)
                     /locus_tag="ACGE4H_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFF8365993.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(494..730)
                     /locus_tag="ACGE4H_002623"
     CDS             complement(494..730)
                     /locus_tag="ACGE4H_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8365994.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(890..1174)
                     /locus_tag="ACGE4H_002624"
     CDS             complement(890..1174)
                     /locus_tag="ACGE4H_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8365995.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            complement(1345..3573)
                     /gene="topB"
                     /locus_tag="ACGE4H_002625"
     CDS             complement(1345..3573)
                     /gene="topB"
                     /locus_tag="ACGE4H_002625"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFF8365996.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSNLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT          981 a          726 c          597 g         1373 t
ORIGIN      
        1 ttttatgaaa aaactatttt gtatgattaa acataaaaaa atcagcatat tccttaatga
       61 catgctgatc taattgagaa ataaagtttt taattcgttc attttattgc ggtttgtcgt
      121 aaataactgg ttatcttgga ctttagtgaa tcgaaatcca atgaactagt ggtaatcatt
      181 atgataaaat caacagcttc ttgacgagaa aaagcaactg gacaattatt caattgaaga
      241 aacaacaaca tggcaacaag agcagttcgt ttattgccat tatgaaaagg atgtttttta
      301 gctaaattaa ttgctaaaat cgccgctttt tcatagattt caggatagag ttcttgattg
      361 aatactgctt gtgaaggctg tttgacggcc atatctaatg catttgcatc ttttacaccg
      421 atcatttcgc ctggcgagtg acgtaaaata actttcgcgt tgattttgat aatttcttta
      481 gtcgaaagat acctcataaa tcaaccaaat cttttaacgc gtcttcatat tgattcatga
      541 tcgaatcaac taatgaatca atatcaggtt cttcttgtgc tttgatcatt cgtacagaac
      601 catcagattg aaaaacataa ctgattgcgt cacctgtctg cacacccatg aattctttta
      661 cttctggtgg gatagttgtt acatcgctat tgccagattt tctaatttta tactttttca
      721 tttttttcat atttcgccac cctctatacc attctcattc tatagtaagt acattatatc
      781 caagtatata cttttaatca atgtatatac ttttattttg ctttttctta cgaaaaaatg
      841 acaaagaaaa aagtttgtga catgactttt gtcacaaact ctcgtattct taaccaaaat
      901 attgatgaaa tacttgtgta ataactggta gaaaataaat aatgatcata gccgctacca
      961 ccaccaacaa ttgttttcgc tgtttctttc gctttagcat gcttgtcgtt ttatgaaaaa
     1021 aataagtagc gcttttagct cctgcagtca aagtcgataa aatcaacgtt ggtgaatatg
     1081 ctgtttgatt gtgcgtcaat gacaacaact tcgtcccgta atcaggatac tttagaaatg
     1141 cttggtacac agccacaatc gtgattgctc ccataagata atttaaaaat gaatgcactt
     1201 ttttcaaata aaaaaactcc ttgctatcta tataaaatca gcctacccca acgtttttgc
     1261 ttgattcact gatatttttt agttttattg gtatttatat tctcactttt cggaataaat
     1321 attccgctat ttcattgttt tcatttactc tacttcgttt tcattatttt ttcttgatgt
     1381 ttctttgttt ccatcaactg cttttgttcc actgttttcg atccatggaa aagaaaaacc
     1441 gattgtcata tcttctttta atactaaaac tgcatcgaat ttcccttttt taccagtaaa
     1501 acctttgaca actttacttg tctttccttt tgaaagcaaa tctctgagat tcgttttagt
     1561 cagcttcttt ttagctactg ttggccacaa tttgaagtcg cattccggat tcaagcatgt
     1621 agcgatttta ggataaagca tgactattcc ctttttacat tttggacatc ttcctaattg
     1681 atcgtttttc ttctccatcg cttttatctg acgtgtcccg ctaatctcat ctgaaaagtc
     1741 taccttgtcg atttgattag gaacttctgc caataaatgt aggatgaatt ttttgatatt
     1801 ctctaaaaaa acagatggtg tcccctcttg tttaccaatt tttctcaagt acccttccca
     1861 gcgcgcagtc atttctgcgc tcgtcagtaa tggttcattg gctactgcct tacaaagagt
     1921 gattcctttt ggcgtaacag ttatttcgtt tttattgact tcgatgtaat gcttctgctt
     1981 aagattttca atgatattgg ctcgtgtagc ttctgtccct attccttcta cttctttgag
     2041 aatttcttgc gcttcatcat tatccagcgt ttttcccgca gttttcattg ctgtgatcaa
     2101 cgttccttcg gtatagggtt ttggaggttg tgtttctttc tccatacttt tcagttctac
     2161 ttctgcttct tcattttcta cgataggagg aagagtctga gcttctttct tttttgactt
     2221 gatgcgtaag atttcctgcc aaccttgctg taatgggatc tttccgatgc tttttagctt
     2281 taattgctgt acgcccgtca ttacggtcgt ctcttcgtag atataatctg cagcaaacat
     2341 agccacagta gtgcgcaaaa cttgcaaata gattgcttgc tgtaatttgg aaagcttcgc
     2401 aaagcgtgca ggagtcggaa tcgtctttgt cggaataatt gcatgatgtt cttgaacttt
     2461 tttcgcatcg acatatcgtt tctttgcttt gatctcagtt gtctgtactt cttctattcc
     2521 taaaaatttt ttataatttt gcagatgttt aaccagatac tgatgctcat tttctgtaat
     2581 gtaaaaggaa tccgttcttg gatagcttag aaacttgctt tcgtacaatc cttgagcagc
     2641 atcaagtgta gctttggcac ttgctttcat tagctgattc atctttgtct gcaaattaga
     2701 tagcgaaaat aaacgtggac tatgcaacct cttttccttt ttcgtaactt caagaatccg
     2761 tccgttttgc ttacctaaat gcgctccaag tttttctatt tccttcgtta actcttcttg
     2821 ggttttaaat gctttttttg gtacgatttt tccagaaaac tgccctttgt ttgtcgtaat
     2881 taaaccttct ccttcaaagt acgtttcttt tttgaagtgt ttgatttctt cttgcagctg
     2941 ataaatcata tagagtgtcg gtgtttgtac acgaccaaga gaaaaactgc catcaatccc
     3001 ttttcgttga agtagcaagc tatataatgg acttccattc attccaatca gccaatcagc
     3061 gttctgcctt gcttgcgctt ctttatattt tggaaaatag ttctttccag gttggagatt
     3121 cttaaaacct tcatagattg cttctttttc aagagaattg atccataaac gtaaatagtt
     3181 cttttcctta gaaaatgcgt ttgcttgtcg aataatcgac cacgcgatgg cttcaccttc
     3241 tcgatcacca tctgttgcaa taatgatggt atcggctgac tgcaattctc gttttacgat
     3301 cgaaaattgt gcacgtttgt ccattggaac ttcgtaacgg aaggtttctg ggaaaatagg
     3361 caaattttct aaagaccatt ttgcccaacg ctcttcataa gctcctggag caaccatatc
     3421 taccaaatgt ccaaaaccat aagtgatggt tacttcacca gcaaacaatg gatcgctgat
     3481 ttcataatac ccttcatgcc gcttttgctg ttggaaggtt tcggcatagg cttttgcttg
     3541 tgaaggtttt tcagctagta tcaccgtctt catcttctga cctccgttac ttaatttttt
     3601 gcggacttct ctattatgga acatattttc gcatctggca ataaaaaaaa gataccgtat
     3661 agtcgaaata gacacaa
//
LOCUS       DAXWLB010000201         3662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXWLB010000201 DAXWLB010000000
VERSION     DAXWLB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.204"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..334
                     /locus_tag="ACGE4H_002626"
     CDS             <1..334
                     /locus_tag="ACGE4H_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFF8365997.1"
                     /translation="FYKDKKAIVIEGPLVRTFRHCLFCKESAKQIEKNGKKISMIILN
                     RSGNKQTYLRLRKQHYHCQSCGKYFTAHTYLVAPSCFISKQVHYTILEELTERQALKT
                     IAKRCDFQ"
     gene            491..748
                     /locus_tag="ACGE4H_002627"
     CDS             491..748
                     /locus_tag="ACGE4H_002627"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8365998.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            835..2073
                     /gene="istA"
                     /locus_tag="ACGE4H_002628"
     CDS             835..2073
                     /gene="istA"
                     /locus_tag="ACGE4H_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFF8365999.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            2063..2803
                     /gene="istB"
                     /locus_tag="ACGE4H_002629"
     CDS             2063..2803
                     /gene="istB"
                     /locus_tag="ACGE4H_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFF8366000.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            3222..3377
                     /locus_tag="ACGE4H_002630"
                     /pseudo
     CDS             3222..3377
                     /locus_tag="ACGE4H_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3444..>3662)
                     /locus_tag="ACGE4H_002631"
     CDS             complement(3444..>3662)
                     /locus_tag="ACGE4H_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HFF8366001.1"
                     /translation="IKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKS
                     IMIFIIYLPFVYFLFPDIRNEVRKIIRK"
BASE COUNT         1199 a          722 c          705 g         1036 t
ORIGIN      
        1 tttttataag gacaagaaag ccatcgtaat cgaaggtcca ttagttcgca cttttagaca
       61 ttgtctattt tgtaaagaat ctgcaaagca gatcgagaaa aacggcaaga agatttcaat
      121 gattatctta aatcgcagtg ggaataaaca aacctatttg cgtcttagaa aacaacatta
      181 ccactgccaa tcatgcggga aatattttac tgctcatacg tatcttgtcg ctccttcatg
      241 ttttatttcg aaacaagttc attacacgat attagaagag ttgaccgagc gtcaagcatt
      301 aaagacaatt gcgaaacgtt gtgattttca gtaacgacag ttcaacggac attatctgct
      361 ctatcggcaa cgctgcgaca gaagctagac tatttacctc attgtttact attcgacgag
      421 tttcgctctc tttcaatttt ccatgggaag ttctcctttt cttgtatgaa ttgagagaca
      481 gggaagctct ttgacatttt gtccagccgg agaaaaaaaa gatttgattg cttatttcat
      541 gcgttttaca agaaaagctc atatgggggt tcgatacatt gtcacagata tgaatgcccc
      601 ttatttttca ttagtcaaag aacccttccc aaatgcacaa atcatcattg attgctttca
      661 cattgggcaa cacttgaaca agtttttcga tcgtgttcga aaacgtgtga tgaagcagtt
      721 gaatcaaaaa tgtcaacggc acctttaaaa tgtaggaaaa cggcaattta aaattgttgg
      781 tttacctaca tttttgtatg ctcttcttat cccaaatgaa atggaggctc agggatgaga
      841 aaagacatac gcgaaggagt gatgatctac gtgatcaatg aaatcaaacc caattatgct
      901 gcactagcaa aacaatacga ctgcgactac cgaacagtta agcatgctta cgaagaagcc
      961 caagttaaag aatcaaaacc tccagaacgt aagaagcgtc caagtaagtt agatccctac
     1021 agagaaatca tccaagacaa aatcaaagac caatgcagtg cttacagcat tttcaagttt
     1081 attgagcaca aaggatttgg aggaagctac agtttagtaa agacctattg tcgccagatt
     1141 aagcaggcga aagtacacaa ggcaaccgtc cgagtcgaac atagccctgg gctttctgct
     1201 caggttgact ggaaggaaga tatgcgacta atcagtaatc agggcgaaat ttttgagttc
     1261 aatatctttt tgtacgtctt gccatattcc aagaaaaaat atgtcacact aactttcgac
     1321 cgaaaacaag acacactctt tgagtgctta gatgatgcgt tttatcaaac cggtggcgtt
     1381 cccaaagaaa tctggttcga taatatgaag acagtcgttg accattctcg tagtcagttt
     1441 tccaaaccgc atttcaacaa acgtttttat gctttcagta aggatgccgg cttcacacca
     1501 attgcctgtc ggccatttcg tccgcaaaca aagggctgtg tcgaagcttt agccagaact
     1561 gttgaccgcc tacgggtcta taatcatgaa ttttatgatg ccgttgagct gtgtcattta
     1621 gtgaataacc tttgtgttga gttaaataac gaggtttctc aatcaactga tcaaacacca
     1681 gatgaacgtt ggcagacgaa cgaaaaagag cacctgcatc cataccaaaa aaaccttttg
     1741 aatccctatt ttgaagactt tattcgccgt aaagtcacca aggaagaaat ggtcattttc
     1801 cgtaaatgca ggtactctgt ggcgcctcgc tatatcggta aagaagtcga aatcgaactc
     1861 tccgataaag aagaccaagt acaaatctat tataacggag aactgattcg gtcgcatcca
     1921 ttaacgacaa aacagctgaa ctaccacgag gaagactatt tccaaatctt gaaatccgat
     1981 gtcatgagtc acaaagaaga cgatggaatc agagcgtata ctcgtgaaag tctcaagcaa
     2041 tatgatctgg tggaggttgc cgatgaagaa tagccattac caagaactga tgaccaactt
     2101 agagactctt ggtttgaatc atatgcggga atatgttcct aactacatcg aagtggccaa
     2161 tcgagaggag ctttctttaa cggaagctct tctcgagctg acacgcagag aagttgatca
     2221 tcaaaataag caaaaaatga atcgtgtggt ggcacgcgcc cgcttttcca agagaaccag
     2281 cctggaagag tttgattttg ccttccagcc aagtatcaat aagcgcgaag tgctagactt
     2341 aaggcacatg ggctttgtgg aaaaacatga gaacttggtt tttatcggca gtcctggtgt
     2401 aggcaagaca catctgacga ttgcattggg aatcgaagcc tgtcgtcaag gctatcggac
     2461 actattcatc acctgtcatg agctgctttt gcgtctgcga acagcctatg aaaaaggtac
     2521 cctagagcgt gttctcaaac gatacgctcg ctatgacgtc ttgattattg atgagattgg
     2581 ctatcttccg attcaaaaac aggaagctga tttattcttt cagcttcttg ccatgcgcta
     2641 tgaacaccgc tctacgatca ttacgacgaa cattgtctta tctcgttggg gggagttgtt
     2701 ccaaagttct gagatcgcag ctgccatcct tgaccgttta gtacatcacg tcaaggtgtt
     2761 caagatcaca ggcaagtctt atcgtatgag agacaagatc taaagtgtcg attgtcgaca
     2821 attactattg ccgaaaacct acatttttat cctgcccaaa acctacaatt ttaagctgcc
     2881 cttgacatca aaaagacact tcacaagcta agtgctatcg acaattgaaa cctttgtata
     2941 aattacttct ataatcagat gacgagttgg attatacaac attcaagaaa tggcagaatt
     3001 ttcaatggcg ctatttaaca gaaagtgaag tagttgattg tctaccctca atctcttatg
     3061 aacttaaaat ggcttagcag tattatcaaa agctgcttaa caattatcac aagaaacagc
     3121 tagctacttc gagaaatgac caaagaagtt ccctcgaaac ttcactatct taaaaagcct
     3181 ttttcatatc tgaaaaaggc ttttaactag cattttcgaa aaagtatttt aatacaaaat
     3241 tagagaatct tcgcacacat atcaaaacat tgaaacgatt tgcttatgga ttttgcagtt
     3301 ttaccaatat gcgcttgtgg atttttctaa tcaacgggtt aataagctat gcattaaaag
     3361 ctagtaaatc ttactaactt tgttgatcat aaatgacctt atcagcccga tttgacaaag
     3421 aactactttt ttacaacatt attttatttt cgaataatct ttcttacttc atttcgtata
     3481 tctggaaaaa gaaaataaac aaacggaaga taaataatga aaatcattat tgatttaatt
     3541 gccatatcta aaaatgaaga agagaataat tgtggaagaa agtaataggc cgaagtagtc
     3601 aaagtaagat tgactataaa aaataagaca acgttcttcc caaagaaata ggatattttt
     3661 at
//
LOCUS       DAXWLB010000202         3633 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXWLB010000202 DAXWLB010000000
VERSION     DAXWLB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.208"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..355
                     /locus_tag="ACGE4H_002632"
     CDS             <1..355
                     /locus_tag="ACGE4H_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFF8366002.1"
                     /translation="SYNLYLSVQIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYT
                     ISVPNLLKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMT
                     THWREELDKQLESYE"
     gene            348..2072
                     /locus_tag="ACGE4H_002633"
     CDS             348..2072
                     /locus_tag="ACGE4H_002633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8366003.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2069..3520
                     /locus_tag="ACGE4H_002634"
     CDS             2069..3520
                     /locus_tag="ACGE4H_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFF8366004.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         1317 a          586 c          715 g         1015 t
ORIGIN      
        1 gagttacaat ttatatttat ctgttcaaat caaagggctc atttttttag ctattgattt
       61 tattttactt tttgccttgc tctgcctatt tgctacttat caatacagta tgattttaga
      121 tagtgaatat acgatctctg ttcctaatct gctaaagcta agttttattt cggttttttc
      181 cagcttttcg tcttttctaa aaataattat tggtagcggt ataattttag gcgtgacttg
      241 gcaattcaaa ggattgatcc tgtttggtgt cattggtctt ttgacggttt ggaatggtac
      301 gatgacaaca cattggcgtg aagaattaga taaacagttg gaaagttatg aataagtttc
      361 ggattctatt ttcaaaagga accttactaa atcgtctgtt gcggaaatac gcggttatca
      421 tgatcggtgt aacgatgact gctaccgtta tttttagtat ccatacttgg gaacaaaatc
      481 aaaaacaggc agaaaacatg acaagtgatg ccgttcaaag cacaagcaga atgctgaatg
      541 ataaaacaac cttgagtagg atcatcaaaa accagcttgt agggaattcc gaaaagatcg
      601 aaaatgtgac gacctactta acaaaaccca tagatcagta tctgatgtat gtatacgaac
      661 agcaaaacag taccgatgaa ttagtttctt tcccaaatca aatcaaagat ctctacgcaa
      721 attatgaaga actaagtgcg atatatatcg tgttgaatca actaccggag tattatgaat
      781 cttctagaga aaacaaaggt ggggaaataa aaaaggggac accaaaatta ctagacgcct
      841 tttatatcaa gttgcctatc acacaaggag gagctgagtc aggaagtatt tttattggtt
      901 ttcaaaagaa agaactagat atgattttag aaaatctaac gtcattcaat ggactctctt
      961 tatacatgat ttctggaacg acaaatcgac tatacacatt ccatgataat aaaatagcta
     1021 aagaaacgtt tgaaagacaa gaaatgatca tcacagagag tctgaagaaa aattcacaat
     1081 tgccgattga cgatctggaa aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt
     1141 atcgtatatt agctgtacta gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg
     1201 ccccgctgat cattggtatc attattttag acaatatact gcttatcttt ctttataaaa
     1261 cattcaaacg atattcacag caagtatcta tcgtcatgca ggccatggat caaacagcag
     1321 cagggaattt agagacgcgg atcgacataa caaacacgga atatgaactg aaggaacttt
     1381 caatcggtat caacgaaatg ttggatagca tcaatcagtt tgtagaagac atctacaaat
     1441 tagaaatcaa acagcaagac gcacatatgc gggcgcttca agcacagatc aatccacact
     1501 ttttatataa tacattagaa tatatccgaa tgtatgcaat cagcgaagga agtgaagaat
     1561 tagcagatgt tgtgtatgcg ttttccgctt tgcttcgaaa caatacgaat caagagaaaa
     1621 cgatcacttt aaaagaagaa ctggactttt gcgaaaaata tgtctatttg tatcaaatgc
     1681 gttatcctaa tcgagtagcc tatcatttta tgatcgatcc tgatttggaa aagatcgaag
     1741 tacctaaatt tgtgatccag ccattagttg aaaattattt taaacacggt attgatttta
     1801 ctcgattcga taatgcgcta agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca
     1861 tcatcaaaga taatggaaaa gggatgactg aaaaacggct aaaacagatt gaagaaaaac
     1921 tgtctcatcc taaagtggaa cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt
     1981 tacgtgccag cttcggcagc agttattata tgtcgcttga gaataatgag acaggtggac
     2041 tgaccgtttc tattacattc aaggagggat gagcgtgtat caagtgttat tggtagatga
     2101 tgaatacatg attttgaatg ggttaaaaaa gataattgat tggcaaagtt taggctttca
     2161 aatagtagca acagcagaga atgccaaaga aggactagct gtacttgaac agcgacagat
     2221 tgatttagta gtaacggatg tgacaatgcc agaaataaat ggactggaat ttattgaagc
     2281 ggctcaaaaa gaacgtcata attttgaatt tatgatttta tcaggatacc aagaattcga
     2341 ttatttgaaa ggaggtatgc aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa
     2401 gttcgaattg gttgaaagcc tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca
     2461 aaaaaatcaa caagaaatct atcaagaact gttattcagt caatggttga atgaagaatt
     2521 ggatgaaata agtgaagaag aaataatagg acaaataagt gagaagcagc ataggatagt
     2581 attgattcaa ctggcaagaa ctcatgatac tcttatcaat agatggttgc aagaacggca
     2641 acaaatcttt tactatcaac gaaattacgg agaccgtgta ttgatcacgt tgttgttgga
     2701 ggcagatgaa gttgatggct tctgttgttt tgttcaagaa aactttcctg atcaggaatg
     2761 gctgatcagt atcggggaag agacagattc tttggacaaa atccctgaaa cattccaaca
     2821 agcgaaagac aatctacagt tgcatcaatt ttatggagat aaagacgagc atgtttttta
     2881 cgcagtacaa gcagaaacaa aagatcaaac catcgatttt tcaagtttca gacgcgtgct
     2941 gcaaaataaa caattggaag tcgctcaaaa aatgatcact gatttttttg aacagtttca
     3001 gttagcagcc atgatgccgg aagatatacg atattccagt tttttactgt ttatggagat
     3061 tcaacgggaa ttgattgatc tggaagatga ggaatattta caaggaatcg aaaaaatcca
     3121 tcaggcaaaa acggtgcaag aacttcagca gctacttctg tcattcatca aaaagcatca
     3181 gagacagaaa aagtacagtg acaatgtgga aaaagtaatc gtaatcctgc accagcatta
     3241 tcaagaacca cttacactca aagaagtgtc agaaaacctg catttaaatg tcatgtatct
     3301 aggacagctg ttcaaaaaag aaacgaaaaa aagtttttca gcttatttga accatttacg
     3361 tatggaaaaa gcgaaacaac tgcttttgca cagtaatcaa aacatcaatg agatagcaag
     3421 tgagattggc tacaacaaca ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag
     3481 atcccctaaa gagtaccgag aaaacagtgg agaatcataa aagaaaaagc caatgtaatg
     3541 aaataactta taaaaaattg tctagaaatc taattttggg ctgttcttat tacttgaggc
     3601 aaggcagctt tttcatgacc tctcgataaa cgg
//
LOCUS       DAXWLB010000203         3631 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXWLB010000203 DAXWLB010000000
VERSION     DAXWLB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.209"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            74..2272
                     /locus_tag="ACGE4H_002635"
     CDS             74..2272
                     /locus_tag="ACGE4H_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366005.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(2730..3602)
                     /locus_tag="ACGE4H_002636"
     CDS             complement(2730..3602)
                     /locus_tag="ACGE4H_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFF8366006.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
BASE COUNT         1307 a          596 c          703 g         1025 t
ORIGIN      
        1 tcatttaatt agatgagtat caattttaca gaaaaatgaa agccttttat tttaaaagga
       61 ggagaggaag aaaatgaaga aaagtacagt attttataaa atatttttac cgatgttttt
      121 attaggagcc gtacttgtca taggatttag tttattcatt taccagaata cctatgaatc
      181 gatcgaatcg agttatttaa cagacaaaaa gaatttgctc aggcaagtta aaactaacgt
      241 tgaatggaaa attagaacga ttgaatattc gttttctacg tatggttcga ccaaaaattt
      301 ctcggacata ttcaagcatc cgctgacata tacagattac tccacttatt ctgaaatcag
      361 gaaagaacta aattttatag aaacgattgt tatggatgat aataattatg atttagtcag
      421 tttggctggt aaatggggag taatcaatgg atcgttgagt caactgaccg aagaggaaat
      481 tgaaaagtat caacagaagt acataaataa caaaaataat ttattttggc aaaagaaaaa
      541 caaaggaatc gaaatggtga tttctttgcc aatgtttgaa aaagaaaaat atgcattggg
      601 aattgctacg atagagaaac atacaataga acaagttgtt ggtgaaagtg gagaagacat
      661 actttctatt gaaaacgaag ttggcaacct ttttactact cagacagtaa aaaataaaga
      721 gcaaccaact gtattaacgg acgtggatct agattacaat cagctgaaag tgataagaga
      781 aaaagataaa acgtatatag tactgaaatc agattataat aattggattt acagattaga
      841 agttgactct agtgcaattg gcgcaatgat ccgcaatctg agaatcgggc tagttgcagt
      901 gtctttaaca ctggtaggac tcattggtct tctttcctac ttattttcgg acagatttgc
      961 ccgtccgatt tctcaaatac aggaacgtct gaatcttaaa tcaaaaggat tcagtgggaa
     1021 agaactagat ttggtcgctc aatcagtcag tcggatcata ggagaaaaag aagcgttaag
     1081 tgcacactta gttacccaaa agccacagct ggaaacgcta tttgtactta gtttatttag
     1141 aaatcgagta gaaaggcgag aactagatca gcggttgcag caatttggtt atagttctca
     1201 aaatgactgc tattatacag ctttggttca aatcgatctg ttagaagata gtcacggtgg
     1261 ggagcgtgat ttgctacttt tagcgatcaa caacatgatc acggaaattg tgccaaagga
     1321 aaatcgaatg ctgccgatcg ttttgaatga agaaatgcaa gccactattt atcgaatgga
     1381 taaagatgat gcagaagcaa gcaaaaaagt catggagtat tgccgactca ttcaaaaacg
     1441 tgtaaaagaa tacttgaatt taactatcag tataggcatc agtaaccggt tcgaaacgct
     1501 gaatgaaagc aagcaatcgg ttgatcgggc aaaagaagcc ctgtattatc gtgtaaatac
     1561 tggtccaagt tcaattattt tttatcagga aatcgttcct gctgctcatg aaaaaacact
     1621 tattagatat ccgatagaag aacaaaatcg attgtttgaa gccattcgtt ctggaaatca
     1681 ggaagtgaag aatttagtac atgaattgat tgatgcattg tttgcacaaa ataaaaatcc
     1741 tttgagtcgg gaagtagttt cggtccgctt gatcaatgaa ctcgttcaat taggacagtt
     1801 attgggagta gattcaaaaa actttgataa catgaaacaa atttatgtaa aagcactgaa
     1861 caattatcat cccaaagaac tagaaaaact tgttttagaa caattagttc tgccaatcac
     1921 agaatgctct caagcaatca ctgatcatga atttaaaaat ttgtcggaga atattatgca
     1981 tatcgtctac acagaatatg atcgtgatct gtctttagat gtgatcgctg ataggttgca
     2041 ttataaccct aactatttaa gtaatgtatt taaaaaggaa acaggagaaa aatttggtga
     2101 ttttgttcaa aatcatcgtt tagaggtagc aaaaaaatgg ttgaaagaga ctaacctctc
     2161 cgtaaaagag attgcagaaa gattgcaata tcgaaatccg caaaatttta ttcgtttctt
     2221 taaaaagaag gaatcgatta cgccgggaga atatcgaaaa aaatatttgt gaatcaagag
     2281 gctgtttttc ttgtggactg attcaagcaa taaaagaagt tagaataacc ctagtatttg
     2341 tatttatgga tagttaaatt tcaaggctaa gcacgcaaaa agaactgtga caaaagtctt
     2401 gtcacagttc ttttttacga ataaatggcg ttcaagagac aggctatcga caataaggca
     2461 taattttgtg aaatatggga agctattttt caaaatcgct tcttattgct cctgtgggtt
     2521 actcgtcgca atgtttgtga gagtttttat catcgctcaa ggctgaacgt ctctttcaca
     2581 acctctgata cacggtgttc caggagtaga ttcttcgaaa aataagccga aatttcacaa
     2641 aaatttaaga agcaattttc gtaaatgcct tcttatttct tgaagctgac cacttctgac
     2701 acaactacca ttttgcgttc agtacataat caccctttga cagaacctac gagtgctcct
     2761 ttgacaaaat atttctgaac aaaaggataa acaatcagca taggcactgt tgcaataacg
     2821 ataacagcca ttttaactgt ttgtgctggt ggaatcacat caacggcact actttctgct
     2881 tgcatcccac ttgatacaat gacgatttgg cgtaaaagaa tctgtatcgg ccatttagat
     2941 gaatcgttga tatacaaaat agcagtcata taagtgttcc agtaagtaac agcataaaaa
     3001 agcgagatag tagcaataga cggtaacgct aatggcagca taattttgaa aaatacacca
     3061 aaatctgtgc aaccatccat ttttgctgat tcttccaaac tatccggcaa ggcttggaaa
     3121 aagtttctca taatgatcat attcattgca ttaacagcca caggcaggat catggcccag
     3181 taagaattga ccagccctag agaacgaaca actaagaacg ttggaatcat ccccccgctg
     3241 aacaacatag aaaatacaac aagaaagttg acgaaacctc gaccaaacaa atactttctg
     3301 gaaagaccgt aggccatcag tgaagttgca cacatactga cgatcgttcc gacacctgta
     3361 acaccaatcg aaacagcaag tgctctaaaa atggtcggcg tagaaagaat gtagcgatac
     3421 gcgtctaaag aaaaggtagt aggaaaaagg ataaactttt tcgcgacgac ttcttgtgta
     3481 gaagcaaaag atgtagcgat gatatttaaa aaaggaacga tacagatcaa agctagcaaa
     3541 attagaaacg tattgttgaa aagtgtaaaa cttctttctc ctatagagac tttctttttc
     3601 atatagatag gctcctcctc aaaataaatt a
//
LOCUS       DAXWLB010000204         3565 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXWLB010000204 DAXWLB010000000
VERSION     DAXWLB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.210"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            118..1857
                     /gene="yfcC"
                     /locus_tag="ACGE4H_002637"
     CDS             118..1857
                     /gene="yfcC"
                     /locus_tag="ACGE4H_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFF8366007.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2014..3198
                     /gene="iadA"
                     /locus_tag="ACGE4H_002638"
     CDS             2014..3198
                     /gene="iadA"
                     /locus_tag="ACGE4H_002638"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFF8366008.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3309..3512
                     /locus_tag="ACGE4H_002639"
     CDS             3309..3512
                     /locus_tag="ACGE4H_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFF8366009.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1136 a          596 c          824 g         1009 t
ORIGIN      
        1 aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg tataattcat
       61 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
      121 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      181 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      241 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      301 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      361 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      421 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      481 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      541 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      601 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      661 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      721 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      781 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
      841 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
      901 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
      961 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
     1021 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1081 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1141 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1201 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1261 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1321 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1381 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1441 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1501 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1561 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1621 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1681 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1741 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1801 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     1861 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     1921 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     1981 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     2041 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2101 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2161 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2221 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2281 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2341 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2401 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2461 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2521 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2581 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2641 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2701 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2761 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     2821 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     2881 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     2941 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     3001 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3061 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3121 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3181 ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt
     3241 ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag
     3301 gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg
     3361 aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag
     3421 cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag
     3481 acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc
     3541 aaataaaaaa tccgaaccat atctg
//
LOCUS       DAXWLB010000205         3456 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXWLB010000205 DAXWLB010000000
VERSION     DAXWLB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.211"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(264..1238)
                     /gene="sgrA"
                     /locus_tag="ACGE4H_002640"
     CDS             complement(264..1238)
                     /gene="sgrA"
                     /locus_tag="ACGE4H_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFF8366010.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1434..2273)
                     /locus_tag="ACGE4H_002641"
     CDS             complement(1434..2273)
                     /locus_tag="ACGE4H_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFF8366011.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2458..3345
                     /locus_tag="ACGE4H_002642"
     CDS             2458..3345
                     /locus_tag="ACGE4H_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFF8366012.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         1155 a          708 c          522 g         1071 t
ORIGIN      
        1 attccgacag ctacatgaat aacgtacaaa tagattcgtt actgattttt cagagatact
       61 accatgcgca gtgtgcattg tcaattattc ttgctcttat tttccatttg tgttcctgtt
      121 atttcctgtt tctcaaatat gttagtaaac aaaaatagat ctggcaaagc tacaacttcg
      181 ccaggcaata cagcactctc gaatgaaata ttcactcaaa aatctatcgt tttattcaat
      241 tttagatctg ttgcttttat tctttatttc actcgatgga agagagaacg aaaatagctt
      301 gctcctacaa tcaaaatgag taacacaccc atccatgtaa ttagcttgtt caaggttcta
      361 ctaccagttt ctggtaaatc gctttttcta ttatctgttt ctttcgcttt ttcttgatga
      421 tcaattccac gagaaacaaa atcaggctgt tttagcacac tttttaggtt gctccctgca
      481 tttctctttt gttccttagt tggtatgata ggtaccaaat caagttcatt atttactttt
      541 cctgtgctgc tttcgctgct cgtgcttcct gtgctactct cactactcat gcttcctgtg
      601 ctactttcac tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt
      661 tcactactcg tactctccgt actgctttcg ctgctcgtgt tttctgtgct aatttcactg
      721 ctcgtactct ctgtactgct ttcaccgctc gtgttttctg tactgctttc actacttgtg
      781 ctatccgtac tgcttggcgg tccaacaact gtccaactgc ttggtttcaa attctccgtg
      841 atcgtcatct gtccatcata aaaaacaatc aaatcaactt tatctttact cttgatctca
      901 tcaatcgata gttctgcaga aatcattatt gtatactcaa taccagcggt ccattgtacg
      961 tcttttaaat aaacttcccc aataactttt tctgaacttc cattaccatc ccaaccatat
     1021 gttagaacag catgtacatc actagcattc acgcttttgt ttggtttgac tggaatagta
     1081 atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa tgaaagattc
     1141 tcatttgccc gttcatttgc agaagtactg attccaatca ttgctgcaat tatactcagc
     1201 ccaagaataa caaataatac ggtcgctgtt ttcttcattt ctatcactct ccatataatt
     1261 accttctatt tgataaagga attatataaa atgaaaatga tgagaacacg acagtcagtt
     1321 gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat
     1381 caatgacaga cttaaaaaag caagaccgat atcagccttg ctttcttcat cttttattga
     1441 taaacagcag tgtatttctt catccgcttt gttttatatg catagagtaa caaaccaaca
     1501 atcaagacta acaagaataa tgtagttgat atagatgctt cagccccaaa gtttccccca
     1561 gtaattattg tattttgcga ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg
     1621 ctgactggta tacccaaaat agctccttgt gtccaattcc aagtaatatg agcaccaata
     1681 gccgtaaata aacttcctgt actgtagata agtgcgctga aaaagcaacc tgctaaaaag
     1741 atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat
     1801 gcagcagatc caatcattgc tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag
     1861 ccacggaata aaagctcttc tgatgccccc actgcaataa ataaaataaa actcgttatc
     1921 agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa atataatgga
     1981 ctagtaataa gcagaaacac tccaattcca acaacgagac ctcccgctaa atacccccag
     2041 tcttttcctc gaacagaaac accataatct tctattcttt ttccttcgac cttactagca
     2101 agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat tgctccaatc
     2161 cattcactat ttccaataaa catatccgca agtgcattat tgacaagaat tcctaaaaca
     2221 aaaccaacaa agactattaa aacagatcca atcaaccgaa taatcctgat catctttcat
     2281 ccctccttta tattaagttt aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa
     2341 tgttctccat catagagaga atagattttt acgaactaag aatatctaca gcaaccttac
     2401 gaaaatgaaa gactgtctcc ctaattcttc tgttagactg tttttaataa ggaaaggatg
     2461 ggaaattttt tgaaaaaaca actactaatt atgtgccttt cagcatgtgc aattttacta
     2521 ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg aacacttaca
     2581 gttcaggatt tttatgataa cccaaaagtt aaatcaaata atcaaacact attaaaaaaa
     2641 atgattgtct tcaaagcatt tgatgatgta tacggaaaag agatttcttc aaaggaagta
     2701 acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa actacaagga acaattaaaa
     2761 gcagttggcc aaacagaaga gacatataag aaactcttta aacagatgct ggctttccaa
     2821 tacggtttga aagccaatgt aaaattaaca gataaagatc tagacactgc ttggaaagaa
     2881 ttctatccag aagttagtac acagatcatt ctattttcga ctaaagaaga agcagataaa
     2941 gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc ttgttcaagc ttatggaaaa
     3001 aataaaacat taaaggaaac tgatggaaaa atgaattttg attcaaccaa tccagagatt
     3061 cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt
     3121 cctgtaacag atccaacgac ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa
     3181 caggataaag gttctaataa agacaaatac aaatctgagc tagaaaaaat tgctaccgaa
     3241 gcaaaaactc ttgatacgga attcatggat aaaacaatcc gtaaagtgat gaaaaaagac
     3301 aatgtaacta ttaaagatcc ttatgtaaaa aatatattca aataaacaaa tttgacccaa
     3361 ttggggatgt ggcaaaattt gagaagtgat ttttggaaat tccttcttat ttttgaggca
     3421 aaccactttt gccacaactt catcggggcc tttttt
//
LOCUS       DAXWLB010000206         3453 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXWLB010000206 DAXWLB010000000
VERSION     DAXWLB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.212"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..2232)
                     /locus_tag="ACGE4H_002643"
     CDS             complement(<1..2232)
                     /locus_tag="ACGE4H_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFF8366013.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACGE4H_002644"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACGE4H_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          968 a          762 c          548 g         1175 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaa
//
LOCUS       DAXWLB010000207         3400 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXWLB010000207 DAXWLB010000000
VERSION     DAXWLB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.213"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..276
                     /locus_tag="ACGE4H_002645"
     CDS             <1..276
                     /locus_tag="ACGE4H_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFF8366014.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="ACGE4H_002646"
     CDS             419..640
                     /locus_tag="ACGE4H_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366015.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="ACGE4H_002647"
     CDS             656..1498
                     /locus_tag="ACGE4H_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366016.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="ACGE4H_002648"
     CDS             1561..3363
                     /locus_tag="ACGE4H_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFF8366017.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga
//
LOCUS       DAXWLB010000208         3347 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXWLB010000208 DAXWLB010000000
VERSION     DAXWLB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.214"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..572
                     /locus_tag="ACGE4H_002649"
     CDS             <1..572
                     /locus_tag="ACGE4H_002649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8366018.1"
                     /translation="LDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELSKKGY
                     YDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMANSGPN
                     TNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVLEGMD
                     TVEEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(649..1824)
                     /locus_tag="ACGE4H_002650"
     CDS             complement(649..1824)
                     /locus_tag="ACGE4H_002650"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFF8366019.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1906..2286
                     /locus_tag="ACGE4H_002651"
     CDS             1906..2286
                     /locus_tag="ACGE4H_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFF8366020.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2360..2722
                     /locus_tag="ACGE4H_002652"
     CDS             2360..2722
                     /locus_tag="ACGE4H_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366021.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1143 a          544 c          637 g         1023 t
ORIGIN      
        1 aactagattt aaaaagtgaa aaaggaccaa aagctgttat caaaacaaac cgtggtgaca
       61 ttactgttca attattccct gaactagcac cgaagacggt tcaaaatttc attgaacttt
      121 ccaaaaaagg ttattatgat ggcgtgattt ttcaccgcgt tattcctgat ttcatgatcc
      181 aaggcggcga tccaactgga acaggaatgg gtggcgaaag tatttatggc gaaagttttg
      241 aagatgaatt cagtcgtgaa ctgttcaacc ttcgcggagc tttatctatg gcaaattcag
      301 gtccaaacac aaacggaagc caattcttta ttgtaaataa cacaaatgtg ccagctaata
      361 tgcttggaca actagaaggt gctggattcc catcagaaat cattgaagcg tataaaggtg
      421 gcggaactcc ttggctagat ttccgacata cagtctttgg acatgtacta gaagggatgg
      481 acacagtaga ggaaatcgca aatgtacaac gtggtccaca agaccgacct gtacatgatg
      541 tagtgattga aacaattgaa atttcagaat agtttggtat ggtacggagt aattatgaaa
      601 aaggacgaaa cctagtaagg tctcgtcctt ttattttgaa taaatgattt aatcgattgt
      661 ctctaaagca tacttgattc gattaagtcc ttccagtaaa gtttcttctg gacaagcaat
      721 attcaagcgc atatgcccat gaccggaagg accaaacgtg atgccaggat ttaatacgac
      781 ttttcctttc cgaacaagag tgttctccaa ttcttggtcg tctgaaatat aggcggtaaa
      841 atctaaccac attaagtaag ttccttcagg gttcattatt ctcactttcg gaagatactg
      901 ttcaaaaaac tgattaactg tttgaacatt tttttctaga taaggaatca gttggcttaa
      961 ccattctcca cccgtctcat aagccgcttg ggttccaatc agtccaaaag tgttgatttc
     1021 gtgctgttgg ttcatcacta aatgtttttc aaaatcagct ttcatctctg gatttttgat
     1081 aaataccatt gaatttttga ttgcagctag attgaaagtc ttggttgcgg aagtaaaagc
     1141 aactagtaga tcatctaaat taggatcaat cgttagcatg gaagtaaatg tatgatcgaa
     1201 taaagttaaa tcttgatgga tctcgtcact aaacagaatc acttgatgtt ttaaacacaa
     1261 ttcgcttaac tgtttcaatt cttctttgct ccaaacacgc cctccaggat tgtgaggatt
     1321 gcataaaatc attgctttta cattgttttc ttcgattttt ttctccatgt cagacaagtc
     1381 catgatgaaa tgattatttt tttctagcaa agagcttcga acgatgtgac gttgatttgt
     1441 ttctataata gaggaaaatg gaggataaac tgggtcatga atcaaaatag aatcgcctgg
     1501 ttttgtaaaa gtttgtactg ctgcagctaa actggttaac acaccacttg taaaaacgat
     1561 attttctttc gtcaactcca cagcatggtg ttggcgttcc caatgaatga tagcctgata
     1621 tagtttatca gacagagtgg cataaccaaa gattccatgt ttgatgtatt cactaaacgc
     1681 tttagctacc ccaccgggag acaagaagtc catatctgct acccacaaag gaagtaagtc
     1741 atccatttga tatgtctcag caattgcgtc ccattttaca ctttctgtat cttttcttga
     1801 aatctgttta tcaaattcga tcatcttaag acctccagtc attttcgtcg cttctttttt
     1861 tgagctattt acagtataat gataaatgcg aagagaggga agtagatgac ttataaaatt
     1921 ggacaaataa ttgaaggaaa agtgacaggg atacagcctt atggcgcatt cgtttctctt
     1981 gataacgaga cgcaaggact tattcatgtt tcagaagtac aatccggtta tacgaaaaat
     2041 atccattcat tgttaaaagt cggacagcca gtaacagttc agatcatcga cattgatgaa
     2101 tattccaaaa aaatcagttt atctttaaga acgttagaaa aaacaaatcc agctgttcca
     2161 tatcgcagaa aaaggtattt cacgaataaa aatcgtaaga tcggatttac cactattgcg
     2221 gagcagttac ctatttggat agaagaagca ttatctgaac tagaacggaa agaaaataag
     2281 aaataaaaag tgtatttttt ctgaaaatgt gctagaattt ttatgtaagc catagaacaa
     2341 gttgatcgag gtggaaattt tggcaagcaa aagagttgct ggtacaatta tgttgcattt
     2401 ggaggatggt actaaaaaat ttttggtcca ttcaattgat gacaagttgg aatttgcgtt
     2461 agcggaactt tctgaaggaa agactggttt agcgaatata cttaatttct taaaagaaat
     2521 agttcatatc gatgttagca agattagttt gatggaattg acaaatacac atattaaaga
     2581 ggaaaatgtt ccgttgtttg tctttgaaac tgaacaaaag aatcaaagag aagaattagg
     2641 cgaaggatat atttgggaag aaccaggtca aatgcgctct gttcttggaa cttacgaagt
     2701 agagggagtg cctttcttct aaaatacaga ttaagcgaac aaagaagttt gaattttttt
     2761 caaacttctt tttttattgt ttggtccaaa aaatacaatg aaatgttctg ctaaagatag
     2821 ttttttagtg cattttattt tcaaaatatg ttgaaaacgg ttgtataacg aataataata
     2881 attgatcgaa aaatcatttc acaaagtaat gcttaattta tagttgacca aggaagaaat
     2941 acatgataaa tttaacagcg ttgattctga agtggatgtt atatcacatt tctaaaaaat
     3001 ttaaaaaata gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
     3061 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
     3121 acaaacgata agttatgata aatgagttgc tgaaagattt cgaaaaacga aatcgaaaaa
     3181 aacttttaaa aaagagttga caatcgtttg tctctctgat atgatataaa agttgctacg
     3241 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
     3301 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaa
//
LOCUS       DAXWLB010000209         3287 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXWLB010000209 DAXWLB010000000
VERSION     DAXWLB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.215"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..370
                     /locus_tag="ACGE4H_002653"
     CDS             <1..370
                     /locus_tag="ACGE4H_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366022.1"
                     /translation="FCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTDGLILSVF
                     FFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFILFGTLNLF
                     QTTVGAWLLWRSGNLMKIGE"
     gene            374..1138
                     /locus_tag="ACGE4H_002654"
     CDS             374..1138
                     /locus_tag="ACGE4H_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8366023.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1267..2214
                     /locus_tag="ACGE4H_002655"
     CDS             1267..2214
                     /locus_tag="ACGE4H_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFF8366024.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2344..3084
                     /locus_tag="ACGE4H_002656"
     CDS             2344..3084
                     /locus_tag="ACGE4H_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366025.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1081 a          547 c          695 g          964 t
ORIGIN      
        1 gttttgtgcc agccacgtgc taagtctcct tctcagtata tacttaggtg tctattcgat
       61 tcaacagcca attgaggtag agaacattgt ttttcagatg tcgctgacag atggtttaat
      121 tttaagcgtt tttttctttt tgatactgtt tattggtaat attttcggta gcgttgccag
      181 ttttttgaat ttttctattt atccactcct ttcgctagca ttaggagtgg tgggactttg
      241 ttctctgtgc ttatttcccg agccattcag tcctgcattt atattatttg gtacactgaa
      301 cctgttccag acaactgttg gagcatggtt gctatggcga tcaggaaatt taatgaaaat
      361 aggtgagtaa caattgaatc caattatatt gaaacgttct gtccatcata ttccagtgtt
      421 agaagttgtt ctgaaagaat taagagagga acgtattccc gttgtcatct attatcacgg
      481 atggcagaca agtaaggagt tagttttgac gcaaggaaga aaaattgcgc agcagggcat
      541 tcgtgtgatc ctgccagatg cgatgaatca tggggagcgc aaacagccgg tttcttctat
      601 tccatcctat acattttggg atagtatcta cggaaatctg tttgaatttg acacacttat
      661 caagcattta gaaaaaagag gtcttctaca tgatcagctt gcagttggcg gggtttcgat
      721 ggggggaatg acaacatgcg ccttgttggc taaacatcct catatagtag cgggcgtgtg
      781 tttgatgggt tcccctgcac ctttggaata tggaaaagaa atcgtaaggc gcgcatcgga
      841 atataattat gtcgtaccaa aagactattt tgatttgatt tcatgggtta agagctatga
      901 tttgtcattg cagccggaaa agttagctgg acgtcctctg ttcttttggc atggacaagc
      961 tgacgaaaaa atcccttata tacaaacgga acgatttgta gaaaaaaatc ggcataagtc
     1021 ttacggaaaa gaaattgttt ttcgttcctc aaaaacacag cgacatatgg tacaaatacc
     1081 actaatggaa gaagcttcca cttttttagc aaaaaatttg ataaaaaagt ataaataatt
     1141 gaaacggaaa atcactcatg acgatagagg attttccgtt ttttattttg ggctataata
     1201 aactctgtta ggaaaatttg gtataattga tagtaagaat gacaacatgt tagaaaggaa
     1261 gagagcatgc ccactttagc agatgttgca aaacgtgcca atgtttcaaa aatgacggtc
     1321 tctcgagtca tcaatcatcc ggaacaggtg actgacgaat tgaaagagtt agttttctca
     1381 gcaatggctg aactggatta ccgtccaaat attgctgcaa aagcacttgt cacgaatcgg
     1441 tcgcagatca tcaaattatt tatactggaa gaaatcgaca caactgagcc ctattacatg
     1501 aatttgctga tgggaatcgc taaacggatc ggtaaagcgc attattctct ccaattagtt
     1561 acaaatgatg ccttcgacgt cggttcttgt gatggataca tcatcacagg tgtcagaaaa
     1621 aaagacttcg attggatcga ccgtttggaa aaaccagttg tcttgtttgg ggaaaattct
     1681 gaaggatatg attatgtaga cagcgataat gaatatggta cagcaaaagc aacgcaatat
     1741 gccttagaaa gagattatca aacgattatt tatatcggta tcgaagtgga cgagcctttt
     1801 gaaatttcaa gggaaactgg ttatttatct gtgatgaagg aagttgatct tcctagtaaa
     1861 atcttgcggt ttccaaatca ttcgacagaa gtagaaagat atatgacaga aaactgggca
     1921 gattttacag ataaaacttg ttttgtatgt agttcagatc ggctagcact aggagcagta
     1981 agagggatta taaatgctgg aggacacttg cccgaagaat ttggtgtgat tggatttgat
     2041 ggggtatttt tagaccaaat cagttcacct aaattgacaa cgatcaagca agatattata
     2101 cgtatgggtg aagtatgtgg agaaatgttg ttaaaaaaaa tagaagaaaa aggggcgagt
     2161 caaggttatc gtcgtttttt tcctgaacta gttatcagag agacaacgaa ttaagtctta
     2221 ttactatata tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct
     2281 actgaaaaca atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt
     2341 tgaatgaaag aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc
     2401 gtaaaattaa ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact
     2461 gaacagaatc agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc
     2521 gagaaaatcc gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa
     2581 aatttggaaa agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc
     2641 acatcgacag gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa
     2701 acaaggacag agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag
     2761 cgttcttatt ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc
     2821 aaaatttctg aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg
     2881 ttcgatacag aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca
     2941 ttcaatcgaa caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa
     3001 agccatggta tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg
     3061 ggagaaaata gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa
     3121 ggaaacgctg cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca
     3181 aatttttttg agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata
     3241 aaaaaacaaa ctattttcgc agatcgtttg caaaaatagt ttatttt
//
LOCUS       DAXWLB010000210         3281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXWLB010000210 DAXWLB010000000
VERSION     DAXWLB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.216"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..359
                     /locus_tag="ACGE4H_002657"
     CDS             <1..359
                     /locus_tag="ACGE4H_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFF8366026.1"
                     /translation="SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGA
                     GMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVI
                     SWLVTLNVYKHSQRDF"
     gene            923..2185
                     /gene="mobV"
                     /locus_tag="ACGE4H_002658"
     CDS             923..2185
                     /gene="mobV"
                     /locus_tag="ACGE4H_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFF8366027.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            2430..>3281
                     /locus_tag="ACGE4H_002659"
     CDS             2430..>3281
                     /locus_tag="ACGE4H_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFF8366028.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1208 a          436 c          676 g          961 t
ORIGIN      
        1 cttcctttct tttagaaaca acatcatggt tcatgacaat tataatcgta tttgttttag
       61 gtgggctttc gttcaccaaa acagttatat caacaattgt ttcaagtagc ttgaaacagc
      121 aggaagctgg tgctggaatg agtttgctta actttaccag ctttttatca gagggaacag
      181 gtattgcaat tgtaggtggt ttattatcca tacccttact tgatcaaagg ttgttaccta
      241 tggaagttga tcagtcaact tatctgtata gtaatttgtt attacttttt tcaggaatca
      301 ttgtcattag ttggctggtt accttgaatg tatataaaca ttctcaaagg gatttctaaa
      361 tcgttaaggg atcaactttg ggagagagtt caaaattgat ccttttttta taacaggaat
      421 tcaaatcttt ttgttccatt aaagggcgcg attgctgaat aaaagatacg agagacctct
      481 cttgtatctt ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata
      541 aaaattttat tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa
      601 attggcaagg gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt
      661 cccttgctga tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg
      721 gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt
      781 tttggtcggc actgccgaca gcctcgcaga gcacacactt tatgaatata aagtatagtg
      841 tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac atttgtgcca
      901 cctaaaaagg agcgatttac atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc
      961 agctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta gaacgaatga
     1021 tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata aaaatattga
     1081 ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa gaaaaacgag
     1141 gaaagatgct gttcttgtaa atgagttgct agtaacatct gaccgagatt tttttgagca
     1201 actggatcca ggagaacaaa aacgattttt tgaggaaagt tataaattat tttccgaacg
     1261 atatggcaag caaaatattg cttatgcaac agttcataat gatgagcaaa cccctcacat
     1321 gcatttaggt gttgtgccta tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg
     1381 tcaagaactg ttatggctac aagataaatt ccccgagcat atgaaaaaac agggttttga
     1441 gttgaagcgt ggtgaacgtg gctctgaccg taaacatatt gagacagcta aatttaaaaa
     1501 acaaactttg gaaaaagaga ttgattttct agaaaaaaat ttagcagtta aaaaagatga
     1561 atggactgct tatagcgata aagttaaatc agatttagaa gtaccagcga aacgacacat
     1621 gaaaagtgtt gaagtgccaa cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat
     1681 gaaaacagaa aagaaaccaa ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt
     1741 agtgactgct gcgagagata acgataggtt aaaacagcat gttagaaatc tcatgagtac
     1801 tgatatggcg agagaatata aaaaattaag taaagaacat gggcaagtta aagaaaaata
     1861 tagtggtctt gtagagcgat ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga
     1921 aaacaagtct ttaaagtcta aaataagcga tttaaagcgt gatgtgagtt taatctatga
     1981 aagcactaag gaattcctta aggaacgtac agacggctta aaagccttta aaaacgtttt
     2041 taaggggttt gtagacaagg taaaggataa aacagcacaa ttccaagaaa aacacgattt
     2101 agaacctaaa aagaacgaat ttgaactaac tcataaccga gaggtaaaaa aagaacgaag
     2161 tcgagatcag ggaatgagtt tataaaataa aaaaagcacc tgaaaaggtg tctttttttg
     2221 atggttttga acttgttctt tcttatcttg atatatatag aaataacgtc atttttattt
     2281 tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg ttttaaagta ttgaaaaccc
     2341 ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt ataagtgacc aaacaaataa
     2401 ctaaatagat gggggtttct tttaatatta tgtgtcctaa tagtagcatt tattcagatg
     2461 aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa agtgagacca tggagagaaa
     2521 agaaaatcgc taatgttgat tactttgaac ttctgcatat tcttgaattt aaaaaggctg
     2581 aaagagtaaa agattgtgct gaaatattag agtataaaca aaatcgtgaa acaggcgaaa
     2641 gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg tccaatgtgc aactggagga
     2701 gagcaatgaa acatggcatt cagtcacaaa aggttgttgc tgaagttatt aaacaaaagc
     2761 caacagttcg ttggttgttt ctcacattaa cagttaaaaa tgtttatgat ggcgaagaat
     2821 taaataagag tttgtcagat atggctcaaa gatttcgccg aatgatgcaa tataaaaaaa
     2881 ttaataaaaa tcttgttggt tttatgcgtg caacggaagt gacaataaat aataaagata
     2941 attcttataa tcagcacatg catgtattgg tatgtgtgga accaacttat tttaagaata
     3001 cagaaaacta cgtgaatcaa aaacaatgga ttcaattttg gaaaaaggca atgaaattag
     3061 actatgatcc aaatgtaaaa gttcaaatga ttcgaccgaa aaataaatat aaatcggata
     3121 tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat tttatgaccg
     3181 atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta caccgtaaaa
     3241 ggttaatctc ctatggtggt ttgttaaaag aaatacataa a
//
LOCUS       DAXWLB010000211         3275 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXWLB010000211 DAXWLB010000000
VERSION     DAXWLB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.217"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(108..605)
                     /locus_tag="ACGE4H_002660"
     CDS             complement(108..605)
                     /locus_tag="ACGE4H_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFF8366029.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(735..1445)
                     /gene="budA"
                     /locus_tag="ACGE4H_002661"
     CDS             complement(735..1445)
                     /gene="budA"
                     /locus_tag="ACGE4H_002661"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFF8366030.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1458..3122)
                     /gene="alsS"
                     /locus_tag="ACGE4H_002662"
     CDS             complement(1458..3122)
                     /gene="alsS"
                     /locus_tag="ACGE4H_002662"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFF8366031.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT          915 a          719 c          535 g         1106 t
ORIGIN      
        1 cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga aatgcccact gtctttccgg
       61 ctctttttat tcttgatttc tcttcttttt tgtttcaata gacgatttta gttattaata
      121 aatgaaaaag catttatatt aaaatttgct aataaaagat ctacatattt ttctagaaat
      181 gcttgatcga tttcatcata ccctttcgtt actccgcaat cgatatctaa aacaccgatt
      241 aaattacctt cttttaccat tggcactacg atttctgatt gtgcggctga atcgcaggaa
      301 atgtaatttg catgggtttt gacatcatca acgatcaatg tttcttgttt ttcagctgat
      361 tctccacaaa ctccttttcc catcttgata cgtgtgcaag aaactcgccc ttggaacggt
      421 cctaaaatca attcattacc atcgaataaa taatatcctg caaagacagt ctcaggcaaa
      481 gtctcattca acaatgcaga tgaatttgct aaatttgcta accagttatc ttctgcttct
      541 aataatcctt gttgctgtaa taataaaatt tcgtatgcat cttttttctg tttttctgtc
      601 cacatgataa aaaccgctcc tttttgatct atggagaaga ttatagctca gtttttttgt
      661 aaacgctagg ttttctcctc aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat
      721 ctcgctgtct ttttctattc tgcttgttgg atttctgctg ctaaatcagc ataatcaatc
      781 gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct gtacaaggga cgagatgcgg
      841 ctaatctcaa cttttccttc agccacttca aaatccatta catgtccgcc aaatgtatgt
      901 gtatcatcga taaaatgcag gtgaaatccc gctgcagcaa ctccttggaa taattcaggc
      961 gtaaagaacc cgactaatgt tccttggaca ttgctttgtt ggaattctgg ttgagactct
     1021 gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata
     1081 tttttgaata atccagttat cttcactgcc aaaaataggt tttccccggc ttctcttact
     1141 aatatgtctt ttttcaattg ttctccatct gcagtttttg agacagaaaa actagtatct
     1201 ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac ttccatctaa ttgggcaact
     1261 gagccatctg atcttccttg ataagctacg ccatctaaaa agatgacttc accatcaagc
     1321 ccatgcaatg ttcccaatcc cagagttcct ttttctaata tatgagcaat cgtttctgtt
     1381 ccatccatca agccggccat caaagctcct aatgtcccgt gctggtataa gattttttca
     1441 gtcatgtcga cacttcctta atagaattga tctggtaaga ttgtttctcc caatttctca
     1501 ttatcacggt aatcaatcgg aatatcaata attacgggtc cttctgtcgc aaatccttct
     1561 tctaaagctt tttctaattc ctctacactt gtagctcgga ttcctttagc gccaaaagct
     1621 tctgcatatt tcacaaaatc aactggacca aagtctacac ctgacgcccg ctgatatttc
     1681 attttttctt ggaactctac catattgtag tgtccatcat tccaaatcaa atgaacgata
     1741 ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag agaataaaaa tcccccatcg
     1801 ccggaaactg atacgatctg tgtatttgga cgcaccaatg cagcagaaat cgcccatggc
     1861 aatgcaacac caagcgtttg cataccatta ctgaataaca aatgacgagg ttcataactt
     1921 ctaaagtggc gtgccatcca aatataatga ctacctacat ctacggtaac agtcatatca
     1981 tctgttactt ttgactgaag cgtgttaata acttctaaag gatgcaagat acctgcttct
     2041 ccttttgatt gaacaatgtc ccgctccgtt aattttgctt gaagagatgc taaatattct
     2101 tttgcatctt ctgacacttg ttgaggctct aatgaacctg tcaataaatc aagagtggct
     2161 gagatatcac cgatcaattc tctttcaggc tgcataaaag gatcgatttc agctggcgct
     2221 tcatcgatca caatgatccg cgcatctttt tccgcattcc agtttcttgc ttcatattcg
     2281 attggatcat agccaattgc aataactaga tcacttcgtt tcaaaagcat atctcctggt
     2341 tggtttctaa ataaacccac acgaccaaag aaatgcgctt ctaattcacg tgagatcact
     2401 ccagctgctt ggaatgtctc tacaacaggc aatgctgtct tttcaactaa ttgcctgatg
     2461 gctaacgttt ctttttcact tgatcctcgc atacctacta atagtaccgg tagttttgct
     2521 tctctaatgc gttcagctag gtaacggata tcatctgcag aagcagaacc taatttagga
     2581 tcgctcaatg gcttgatgac attaccttgg acaggtgcat ccactacatc ttgcgggata
     2641 ctgataaagc tcgcgccttt ttttgaactc ttagccatac gataagcatt ggcaatcact
     2701 tctgaaattg tttctggatc ttggatttct gcactgtatt ttgtgattgg ctggaacagt
     2761 gctgcattat ccatgctttg atgggtcaac ttcaatagat cgcttcgttt tacttgtccg
     2821 gcaattgcta aaactgggtc accttctgct gtcgctgtta ccaaacctgt agctaagtta
     2881 ctagcaccag gaccacttgt tgcgataact actcctggct ctcctgtaat acgtccgatt
     2941 gcttgtgcca taaatgcagc attttgttcg tgacgagtca cgattaattc aggaccttga
     3001 tcctctaatt cattaaatac accatcgatt tttgcacctg gtataccgaa tacataatca
     3061 acatgatgat taatcaaact ttctactaca gatttcgaac cttgtttcat tttttcagcc
     3121 attgaacatc gtccttttta tgctttattt ttaattatta gttagaaata atcacaaata
     3181 agtgatacag tttctaaacc tgtttcattc aaaagtatag catgttttat ttcatttgtt
     3241 tagccatatg tttcaaaaaa tgaaaaagaa tgaca
//
LOCUS       DAXWLB010000212         3218 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXWLB010000212 DAXWLB010000000
VERSION     DAXWLB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.218"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1438
                     /locus_tag="ACGE4H_002663"
     CDS             <1..1438
                     /locus_tag="ACGE4H_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFF8366032.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="ACGE4H_002664"
     CDS             1776..>3218
                     /locus_tag="ACGE4H_002664"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFF8366033.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAXWLB010000213         3203 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXWLB010000213 DAXWLB010000000
VERSION     DAXWLB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.219"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            680..766
                     /locus_tag="ACGE4H_002665"
     CDS             680..766
                     /locus_tag="ACGE4H_002665"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFF8366034.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1068..1292)
                     /locus_tag="ACGE4H_002666"
     CDS             complement(1068..1292)
                     /locus_tag="ACGE4H_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366035.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1658..1942
                     /locus_tag="ACGE4H_002667"
     CDS             1658..1942
                     /locus_tag="ACGE4H_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366036.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1945..2361
                     /locus_tag="ACGE4H_002668"
     CDS             1945..2361
                     /locus_tag="ACGE4H_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366037.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2498..>3203)
                     /locus_tag="ACGE4H_002669"
     CDS             complement(2498..>3203)
                     /locus_tag="ACGE4H_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFF8366038.1"
                     /translation="ELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFS
                     FEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIE
                     EFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSS
                     LTSDLPKISLRNWLEE"
BASE COUNT         1065 a          599 c          499 g         1040 t
ORIGIN      
        1 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
       61 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
      121 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtactcagat
      181 aaaaagttgt tagtaccctt ctgtatccgt tgggagagta aggctcaact gccccctaaa
      241 aagggttcta aaaaatcttt aaaaaataaa taaaataaac gcgcgcgcgc gagcaaaatt
      301 tcccgccaaa aaatcaccca ctttttaaaa taaacaggtt cgcgcatgat attaaactgc
      361 caatctagct ccctatggtc gctgtaagaa ggagtgatcg ctacgctctc acattttact
      421 tgcagcagtc gcctttaggc tccttcgcca aaaataattc attagggact cctttgtggc
      481 gtcctgagag ctcgtctaag ccgttctaag acaagattcg ataaaaacta tgtccttcgt
      541 tctaaagcgc cttacaggcg gtcagggcaa gccctaacaa ccccaaggca ataaaaaact
      601 taggcttatt tttgcttttt tattgtataa ttgattgtcg cctatccgac agtcttgcga
      661 ctggaaggag gtgtgtttcg tgttcactaa ctttatttgc tcccttcttg ttgggctttt
      721 aagcgctttg ttcagttact ggttgaataa ccgaaacaag aagtaaaact taattaaggc
      781 gactcagccc acaaaaaaag ccccagagaa tccgacctct ctggggcttt cgtgtgtttc
      841 gtgttcactt atttgcaatc aaattataac agaaataaat atttatttca aaaatatcct
      901 acaaaagtct aaaatccgta attatttctt atatgcaaca tttacaaata aagtcgctct
      961 gagagctcgt atttttcgat ttaagacgtt ttatttagtt tttggtgtat ttcattggtc
     1021 caagctaaaa aactcttaga agcgatttta gagccattta aacccactta attatattct
     1081 ccatgaaatt cgctccatgc gtcaaaagct gcgtctgaaa ttccgccgtc ttcgttcact
     1141 agttgcatta atttttccaa cgatcgtttt ctttcgggtg tcatattttt atattccaac
     1201 aatttttcgt ctgttaaatc gaacttgatt gcttgaattg cttgctctaa tttttctaag
     1261 ttttgaaaat attcgtaagg aaatttttcc attttttctt tctccagtct cacaattttt
     1321 ctagaaataa aatcacttct ttgccgtcta tactctcaaa aaaaatgtcg tttgaaaaac
     1381 taaataaatt tttgatcgtg catttttaaa tttggttatc gattacacgt gcagcactcc
     1441 cccccgaatt ttaaagagca taaggaaacg tgtacccact ttctaaaaaa agtgggtaga
     1501 taggctgtgc ctatcacgtt tctcagaaaa aaatttgata tcactttttc gccatcgtgg
     1561 gtagcttatg tatagcattt tggcaaaaaa cagctataca aatatagtat ttgtatggta
     1621 tacataaatt atacataatt gcaaaaggag attttttatg gacattttag ttcggaatat
     1681 tgatccaaag tttgttgcaa caatagacaa acgttgccaa gaactcacga ataaaacagg
     1741 agaaaaatgg agtcgaaata aatatctaaa agttttaatc gaaagtgatt ttgaccgtca
     1801 acttacgact tacaaaaaag accaattcga tttattgctc gataaattta ttgctatcca
     1861 aacagaaaat actcaaatat taagcgagta tgtctacaca aataatcaaa tcattgaaat
     1921 gcttatcgga ggaatgatat aaccatgaga aaaacttctg tccaaaaaca tgttactttg
     1981 taccctagtc atgttgacgt tttagattcc atcatcgaaa aaaatccaga tatcaataac
     2041 tactcggaag ccattcgctt tttatgtatg agttatgaac ccgataccaa cacgaccact
     2101 accagcaatc aggcaaaact aaatgctatg ggaaaagaaa tttccatcat gactgaaatc
     2161 atcgcagaat ttgctgattt atatctaaaa gatacaggcg ttcttattgg cacaaattct
     2221 aaagtatatc aagaagtgaa agaaaaaatc gaagctaaac taaatccatc aaatcgaaat
     2281 cgaccaaaga atcatcaaaa agaaaccagt caaacagtca cttctagaaa tttcgaccca
     2341 acaaattttc taaaacgata aaaaacagat aggagttatt cctatctgtt ttttatttag
     2401 aagccccata caatatgcta gaatgtacta gcaatcttag tcggttgtaa gggacttgtc
     2461 tacttgctta atgtaagggg ggcgagttct tttttattta ttcttctaac cagttacgca
     2521 gagaaatctt tggcaaatct gacgttaggc ttgaaaaagt aaattctaat ttggcgattt
     2581 tattgccctt ttttgctttt atttttgtga ctttaagatc attgaagtat ttttttagtt
     2641 ctcgtaatgc aggatttaat actttttgat caatgttacc catttgatag tattctggga
     2701 tatccattaa gctcctaaac tcttcaatac gaactacata ataaccagta gaacggtact
     2761 gcataagcag tcggaataga gtttttgcat acgtagaacg caacgacgtg aaagcagata
     2821 attcaaatct actaaattca gctgttaaac cattaataat atattctagg tccggattaa
     2881 cactgacttc aacaaacttt tttgttttat tgatttcaaa accagtaaac aaaacaaagt
     2941 agcgaataga atcctcatct tcagtacggt aagtaagatt aagcatatct ttatagagct
     3001 tttctaaatc accgacaaag gcttttgtag cagtcatctt ataatcactt aactctttta
     3061 attcctcgaa agaaaatcta acttttttca aaccacgatc tttcatacga gcgcataatg
     3121 caaaaaataa atccatctgc ttagcattga aatttttcag cggtactaaa ttcaattcat
     3181 tttgatattt gactgttaat tcc
//
LOCUS       DAXWLB010000214         3069 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXWLB010000214 DAXWLB010000000
VERSION     DAXWLB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.220"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            52..807
                     /gene="fabI"
                     /locus_tag="ACGE4H_002670"
     CDS             52..807
                     /gene="fabI"
                     /locus_tag="ACGE4H_002670"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFF8366039.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(917..1969)
                     /locus_tag="ACGE4H_002671"
     CDS             complement(917..1969)
                     /locus_tag="ACGE4H_002671"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFF8366040.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2095..3015
                     /locus_tag="ACGE4H_002672"
     CDS             2095..3015
                     /locus_tag="ACGE4H_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFF8366041.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          893 a          619 c          587 g          970 t
ORIGIN      
        1 tctatttttt tacgactatc aaatttttat cagaaacaaa ggagagtcat tatgtcattt
       61 ctagaaggaa aaaaaatcgt cattatgggc gtcgctaaca aaaaaagcat tgcttggggt
      121 tgtgctgaag caatgttgaa acaaggggca gaaattatct atacttacca aaatgaacga
      181 atgaaaaaat cattagtcaa attggtcggc gaagaagcat tcactgtaga atgtgatgtt
      241 tctagcgacg aaagcatcga aaaagcaatg aatgccatcg gtgaatatgc tggggagatc
      301 cacggccttg tccatgcagt tgcttacgcc aacaaagaag aattgtccgg aaatgtgtct
      361 gatatttcca gagacggcta tcaattagct caagatatca gcagctactc tttaattgct
      421 gtatcaaaat acgcacaacc gtttcttgca ccaaacagcg ggatcgttag catgacttat
      481 ctaggttcag aacgcgcaat cccgaattat aacatgatgg gtattgcgaa agcttctctt
      541 gaagctgcta tccgttattt ggcagctgag ttctcgccta aaggaattcg ggtcaatgga
      601 atctcagctg gggcaatcaa aacattagct gtcacaggtg ttaaagacta tcaaaaactg
      661 atccagttat ctgaatctcg tacgcctgat cagcaaggtg tgacgatcga agaagtcggt
      721 aatgcttgtg ctttcttaat cagcccactt tccagcggaa tcatcggtga tattatctac
      781 gttgacaaag gtgtccatct ttcataaact tttgttgcat tctttaaagt aaaacatata
      841 aaaatcagga aaaaactttt taaagtctct tcctgatttt ttctttttac gtttttttca
      901 actcattact ttgaaattat tccacgactt caatccagcc ttctggtgct tctatgtctc
      961 caaattgaat acctgtcaat tcatcataca accgttttgt tactggtcct acttccgttt
     1021 cactataaaa tacatgaaga ttgttattgt gataaatccc accgattgga gaaataacag
     1081 ccgctgtacc acatgcacca gcttctgcaa attgatctag atcatcaatg aaaatatctc
     1141 cttcaattgc ttgcagtcct agtcgttctt cagctaactg caacaaggaa tatttcgtga
     1201 tacttggtaa gatagagggc gattgcggag tgatgaattt tccgtctttt gtaattccaa
     1261 agaaatttgc tgcacctact tcttcgattt tcgtatgtgt ggctggatca agataaacac
     1321 aatctgagta atccagttct tttgcttctg ttcctggaag taaactcgct gcgtaatttc
     1381 cgccgacttt tgctgcacca gtccccttag gagcagcacg atcgtaatca gatacaataa
     1441 aattcgttgg ttttagtcca cctttgaaat acgctcctac tggcgtacag aagacgacaa
     1501 ataaatattc tggagctgga tggacaccaa tgttttcacc gaccccaatc agtaaagggc
     1561 gcaagtataa tgtagcgcct gttccatagg gcggcacaaa atcttcattt gcttttacca
     1621 cttgcttgac agcatcaacg aacatctttt cagggaccat tggcatcagc aatcgtctag
     1681 cactttgatt catccgtcga ctattttgtt caggtcgaaa gagattgacc ttgccgtctt
     1741 ttcgacgata agcttttaga ccttcaaaac attgctgacc ataatgaagc gctggagagc
     1801 cttcatgaat cgtcaggtaa ttatcttctg tcaattctcc ttcttcccat tgtccgtctt
     1861 tccatttggc aataaaacgc caaggagttt tgatataaga aaaacctaga ttgttccaat
     1921 cgatttgcat atacttacca ccttcgttat tttctccaca ttttatcatt atccatatta
     1981 tttgtcattt tattttcagc ttttttcctt cattatagtt atttttgcta tttccttttt
     2041 tgaaaacttc ttggtacaat acagaaagaa tcaaatttta gaaaagagga ttttatgttt
     2101 tcattattag caactgccgg tacgacagac tcttctggcg agacattgac agatcaagct
     2161 gtcagcaaag taaatgcgtt tcagcggttt tgggacggga ttgattggga cgccattttg
     2221 gctgccttta tccaaaaaag tttatatctg atttttttga tcgtcctttt ttggattctt
     2281 caccgtgtgg gaaaatatct gattgataag tcttttaatc agtattctaa aaaagccatt
     2341 ctaaacgaga gccgcttaaa gactttacat agtcttttaa atactatttt tcattatacc
     2401 cttggtttct tttttatcta tgcaatttta tctgctatcg gtgtgccgat cggatcactt
     2461 ttggctggtg ccggaattgc aggtgtggca atcggtcttg gtgcccaagg gttcatgagt
     2521 gatgttatta ccggattttt tattatcatg gaacaacaga tggatgttgg ggactacatc
     2581 aagctagcaa acttaacgat tgaaggaacc gtcgtatctg ttggtatccg tacccttcag
     2641 ctgaaagcaa cggatggtac tgtccatttc atcccgaatc gaaacatcac cacgatcagc
     2701 aatctctctc gtgcaaatat gcaagtactt ttagatattc ggattgttcc tgaagaagga
     2761 tatgacaaaa tctatgagat cattgatcga gtaaatcgta cattagctga aaaataccaa
     2821 gaagagctcc aaactgaacc ttctatcttt ggattagtgg atcttggaaa cagcaatttt
     2881 gcgattcgta cggtctgcta cgcattgaac ggaaaacaat atgccttaaa agaagagttc
     2941 ttgagctcct atgtcaaaga attgacagct tctggtttca cgattccgaa cagtccaatt
     3001 gctgtcggaa aataatttgg acaaacgatg caacgaatct cactaaaaag agcgtgggac
     3061 aaaagtaaa
//
LOCUS       DAXWLB010000215         3019 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXWLB010000215 DAXWLB010000000
VERSION     DAXWLB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.221"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(239..853)
                     /locus_tag="ACGE4H_002673"
     CDS             complement(239..853)
                     /locus_tag="ACGE4H_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFF8366042.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1459..1836)
                     /locus_tag="ACGE4H_002674"
     CDS             complement(1459..1836)
                     /locus_tag="ACGE4H_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366043.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2136..3017)
                     /gene="galU"
                     /locus_tag="ACGE4H_002675"
     CDS             complement(2136..3017)
                     /gene="galU"
                     /locus_tag="ACGE4H_002675"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFF8366044.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          916 a          631 c          467 g         1005 t
ORIGIN      
        1 ttatttttct gacactacat attcttataa ctcatttctc ataactgtga aacaacttat
       61 aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat cttaaaataa
      121 ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct ttttttcaaa
      181 cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat gtggataact
      241 attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact tgctgcagca
      301 aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt ggcaaagtca
      361 agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc
      421 gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca gtaacaatga
      481 ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc gggtttcttc
      541 cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga tcaataattg
      601 cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca atcaatatac
      661 ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt agtgtgcggc
      721 tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa gtgccagctt
      781 gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa tagtcaaaat
      841 aacataaact catttatctc tcccgcctag ctatacttga catgaataga aacagctgcc
      901 taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg gtctttgctg
      961 tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata gcaaataaaa
     1021 caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct agattaggta
     1081 caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt
     1141 gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc ctttttttat
     1201 tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca agcaagaaaa
     1261 tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa ggaatggctt
     1321 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
     1381 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
     1441 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
     1501 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
     1561 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
     1621 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
     1681 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
     1741 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
     1801 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
     1861 attaaaaaat tcgctttttt attctactat gaatcttctg gcttggcttt catttctctt
     1921 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
     1981 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
     2041 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
     2101 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
     2161 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
     2221 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
     2281 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     2341 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     2401 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     2461 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     2521 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     2581 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     2641 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     2701 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     2761 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     2821 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     2881 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     2941 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     3001 gcttttttga ccttcactt
//
LOCUS       DAXWLB010000216         3015 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXWLB010000216 DAXWLB010000000
VERSION     DAXWLB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.222"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            59..481
                     /locus_tag="ACGE4H_002676"
     CDS             59..481
                     /locus_tag="ACGE4H_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366045.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            500..2092
                     /gene="mobP2"
                     /locus_tag="ACGE4H_002677"
     CDS             500..2092
                     /gene="mobP2"
                     /locus_tag="ACGE4H_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFF8366046.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2104..2439
                     /locus_tag="ACGE4H_002678"
     CDS             2104..2439
                     /locus_tag="ACGE4H_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366047.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2586..2780
                     /locus_tag="ACGE4H_002679"
     CDS             2586..2780
                     /locus_tag="ACGE4H_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366048.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2812..>3015
                     /locus_tag="ACGE4H_002680"
     CDS             2812..>3015
                     /locus_tag="ACGE4H_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366049.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQS"
BASE COUNT         1235 a          442 c          588 g          750 t
ORIGIN      
        1 gtttgttaat tgaataataa aaatagtatt gtgaatttat aaaaggagga aatcaatttt
       61 ggcaaaagca acaagaaaac atgtatatct ttacgatatg catatcgaag tacttgatga
      121 aataatttct actcatgaag atatatctaa ttatagtgaa gcagtcagac atttgtgtat
      181 gatgtatgat ccaaaaatcg aagaaaaaat aaatccaatt caaattaaat tgaatgcaat
      241 gggaaaagaa atttctattt taaccgaaat ggtttcagag tttgcggatc ttcatttgaa
      301 agatacagga atactaattg gtacaaattc aactgtttat caagaagcag aagaacgtgt
      361 ggaaggaaaa attaaatcca atcaaaccaa aaaatattct tctaagttaa agaaaccagt
      421 gaatgaatct gttcatacca gagtagatcc atttgatcca acgaagattc taaaaagata
      481 aaagtagaag gagaacgtga tgaataaatc gcctgcagtt attcttactt gtcaatttac
      541 cttgcccaat aaaaaatctt tttcaacata cattgattat atgacaagag aaaaagcact
      601 ggaagaaatg gaacgacgta cgcccgaaga agaacaggaa ctgcaacgaa tcaagaatgc
      661 tttagaggat ttttatattc ctgaaggaga aactttttcg gaaagtaaga cggataaaga
      721 attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca attttgacaa
      781 gttagaggaa caagatttta ccaaatatct ttcttatatg acccgtcact atgcgttaga
      841 acaaaagaaa gaactgacgc aaagggaaca aaaagaatac caacggttga accaagcaat
      901 gaaaaaatat caaacagagc cacataaaaa agcttcagga aatcttcctg gagttttttc
      961 tatggcttca gacgaagtga aaggaagtga tctgaaggag atcaaacaaa tcttccaaag
     1021 aggacaacaa aaaggctcaa tcatttatca agatgttatc tctcacgaca attcgtatct
     1081 tgaaaaaatg ggcttgtacg atcccaaaac agatacctta gatgaagaag gtttgaaagc
     1141 agctggaaga aaaatgatgg agacattatt cgagaaagaa caattgaatg aaacaggtta
     1201 ctggatggca acgattcacc gtaacactaa gcatatccat attcattttg cagttgttga
     1261 aaaagaaaat acgcgtaaac cgtatactga gattgaaaat ggtgtgagtt atatcgtacc
     1321 gaaggggaag agaagacaag caacattaga cgcaatgaaa accaattatg tacatgagtt
     1381 agaacgtttc tcttatgaaa aggatcgaat gatcggtatg gagaaacgaa atttattgac
     1441 acgaaaaagt gatttgcgta acaccttaac aaaagtagta aaagaaccag cacgttacga
     1501 tcgacacgcc atgaacttat taaatgaagt atataaaacc ctacctgaaa aacgttcgga
     1561 atggaattat ggagatgaaa atcgaaccaa gctttcacca tttacaaggg gaaaacttga
     1621 tgagttaacc aaatatgttt taacaaaaga acctggatat caagaatata tacagatttc
     1681 aaaagcattg aaagaagaag ccaaaagcct ttatggggaa agcaaaagag aaacaaagaa
     1741 tgcagaaaaa aacgccctct ttgatttaaa aaagcgcaca ggaaatgcga ttttaacaga
     1801 actgaagaga caggatagtc aaataaaacc attaatagaa agtgtcaatc agatgcaatc
     1861 tatttacaat gaacgatcct ttcgaacact caaacagttg ccaacaaatg aatacaagta
     1921 tagttatcaa gaattcgtaa agcgtaggaa acaattccaa caaagagttg tcacgcagcg
     1981 aagcttgcgt caattaaatc gaacaattaa tgcacatgaa gaaaaatacc acgcaagtaa
     2041 agcatatgaa gagcaacagc gaagaatcca aatggaacaa gaaagacaat aggaggaaga
     2101 gaaatgaaac aatatttaga tcaatggaaa gtcatagaag gatcactaaa agaagaacga
     2161 atcgaacaac ttcctgattg tttagaaaaa gagcatttgt ttcagataag cgaaatgttg
     2221 agaaacgaac agtttgatcc aaatcaattt ttagttgttg agtacccagc tactggtgtg
     2281 tattgttgta atcatgtgaa gggtgaaaaa tattttatta ttcaagaata cgaaggtaag
     2341 ttagcgcctt attatactac ttgggaaata aatgaagagg gaatcaataa tttcccatgt
     2401 gaaagcattg aagagtctat ttctctcacg gaatgttaaa taaatagaga cgtaaaagag
     2461 tcctgaacgt gattgtccag gactcttttt caatgaattg ttttccgcca attcactgag
     2521 tggtgcgatt caattttatt atatcaaaag aaagtgagat tccaaagtga agggaggaag
     2581 aagaaatgac tcagacaacg attggtgttg tggttgaaaa aagcagggat aatctcgttt
     2641 ttgtgacaga aattcaaaca ggtagagctt ttgtagtcac agacaaagct gcaaaagcgt
     2701 atcaaaatgg caatatttta acgttaaatc tgactacgaa gatgttcgtt gatgcagtag
     2761 aaaattatcc atttgtttag acaaacgata ttttacaagg aggaaacgga catggcagaa
     2821 gaaaaagaac cactagataa tagtcggttg ggaaagtcca aaagaaaatt agtacggttg
     2881 caaaacgaat taaataatca atttcaaaag ctatttgatc accaaaaatt gacaaatggg
     2941 caaccaatga atgacaaacg aaatggtcgt tcatggttta accagcaaga aaaaatagaa
     3001 aataaaatac aaagt
//
LOCUS       DAXWLB010000217         2996 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXWLB010000217 DAXWLB010000000
VERSION     DAXWLB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.223"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(41..2404)
                     /locus_tag="ACGE4H_002681"
     CDS             complement(41..2404)
                     /locus_tag="ACGE4H_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFF8366050.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2569..2996
                     /locus_tag="ACGE4H_002682"
                     /pseudo
     CDS             2569..2996
                     /locus_tag="ACGE4H_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
BASE COUNT          796 a          630 c          552 g         1018 t
ORIGIN      
        1 aatgtaaaaa attctgtttt gcgggatcga ttttatcatt ttattggtca agatttttcc
       61 atttccaatc caatacttct ggcagatcaa taccattttc acgaatatag ttttgatgtt
      121 cttgtaattt ctgatccatt ttttccgcaa aatcttctgc ttgacttcct ttgattttta
      181 aagctgcgtc ttttgccaaa tgataacgat caagttcatt tactacacgc atgtcaaatg
      241 gtgtcgtgat atctccattt tctttgtagc catgaatcat taaatgatgg ttatgacgat
      301 caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa agcaaataag atcggtttgt
      361 cttttgtaaa atattgatca aattcttcat cgctcaaccc acgcggatct tgacttggat
      421 gacgtaattt caataaatct acgacattga tgaaccgaat ttttagtttt gggaattctt
      481 ccagcagcaa agttacagct gctaatgctt ctaagttaga ttccgttcct gcagcagcaa
      541 tgaccaattc aggttcctcc tcttttgctg tcgaagccca atcaatgatt ttcaagcctt
      601 cattcactaa gacggctgct tcttctgctg agtaaaattg agcacgcgga tgtttggaag
      661 agatgatcaa attgattttt tctgacgagc ggaatgcttt atccatgact gcgagcaatg
      721 tatttgcatc cgcaggcaga tattcacgga tgaactccgc cttcttttct gccaaatgtg
      781 tcaatattcc tggatcttgg tggctatatc cattgtgatc ttgctggaag actgttgagc
      841 ttgcaatgag attcagcgaa ggataatctt ttcgccaaga aaggtcatgt gatttccgta
      901 tccatttgaa gtgctgagtc aacatagagt caaccactcg caagaatgat tcgtaacttg
      961 caaaaaaacc atgccgtcca gtcagaacgt acccttctaa gaacccttca tcttggtgtt
     1021 ccgataattg actgtcgatg acgcgaccag atggcgaaag ccattcatca ttttctggtt
     1081 cgattttttc catccattgg cgatttgtcg ttttgaaaac ttgattcaat cggtttgatt
     1141 ttgtttcatc tggtccaaat atacggaaat tttctggatt tttctcaatg atctcgcgaa
     1201 cgaatttacc aagttcggtc atatcttctg ctttaacgga gcctggtttt tcaaattgca
     1261 aagtatattt tttccaatct ggcataataa gcggtcttgg atcgattcca ccattggtga
     1321 taggattttt tgccattctt tggtcaccag taggcgcaat ttctctgatt tcttctaaaa
     1381 tacgtccttg tgcatcaaat aatttttctg gttcatagga tttcagccaa cggagtaaag
     1441 catctgcatg atccatatgt tcttgatcta caggaatcgg tacttggtgt gctcggaagg
     1501 ttccttcaat tggttccccg tcccattctt ttggaccggt ccatcctttt ggtgtgcgta
     1561 cgatcaatac tggccatttt ggcatcacga catctgttgc tgtcttcagg cgagcttctt
     1621 tttggatgga tttgattttc tcgattgctt gatccatttt ttcagccata acaggattta
     1681 atttttctgg atcatttcct tcaataaaga aaggctccca gccaagtccg ttaaagtaat
     1741 ttgctaattc tccatctgat ttacgtgcca agatcgttgg gttggctatt tttgcaccat
     1801 ttaaatgaag gatcggtaaa acagctccat ctatcactgg attgataaag acatttgaga
     1861 accagcttcc agctaaagga ccggtttctg cttcaccatc accaatcacg acagcagaga
     1921 ttacttcagg attgtctaat acagctccga ctgcatgtga aagagagtat ccaagctctc
     1981 cgccttcatg gatagaacct ggtgtttctg gtgctgcatg agaagcaatg cctccaggaa
     2041 atgaaaatcg cttgaataat ttttgcattc ctgtctcatc ttccgttact tctggataga
     2101 tctctgtgta acttccatct aaataagcat tcgataccat gacttgtccg ccgtgtcctg
     2161 gcccttcgat ataaaacatg ttcaaatcat atttattgat gactcggttc aaatggacat
     2221 aaataaaatt ttggccagga atcgttcccc aatgaccgat tggatgtttt ttgacatctt
     2281 ctggtttcag tgtacgtctc agtaaggggt tatctttaag ataaagttgc ccaacagaaa
     2341 gataattagc agcacgccac caagccgtca ttttatcaaa gtattcttta ctagaataat
     2401 ccatacataa cactcctttt cattattact cttattctag cgcagatact cccaattgaa
     2461 acctaataag cacttctgtt catttgtttg gattttatat ctagccaaat gaacaggtat
     2521 aaaaaaccac gaaatttttc ctttcgctga cgttttttac attgatccaa aaaacttgtc
     2581 gttatcacaa tggctgttcc gcagttcatt tcgctcctca tcatgcataa tttgctgaat
     2641 gacgctaggc cagttaacac attgttggaa aactggggct ggatcgagca attcctttcc
     2701 tgattgatcc gttttatgta aaagtctctg ttatctttgt caatatgtga attggtatcc
     2761 cagctactat gctaatttct acagggatta tccaaaattt ggatcgtgac caaatcgaag
     2821 cggcgtaatc ggaaatatca ataacttcaa cgtgattttt cttttaactg tcaacggacc
     2881 gacaaactca aacttttatg gtgcaggttc cactgatctt ctagttactt ggctatacct
     2941 gactgtcact aaaatagatt acaatctagc ttccgttatc ggtatcttga tcttta
//
LOCUS       DAXWLB010000218         2786 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXWLB010000218 DAXWLB010000000
VERSION     DAXWLB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.224"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(74..1168)
                     /locus_tag="ACGE4H_002683"
     CDS             complement(74..1168)
                     /locus_tag="ACGE4H_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFF8366051.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="ACGE4H_002684"
     CDS             1357..2118
                     /locus_tag="ACGE4H_002684"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFF8366052.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACGE4H_002685"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACGE4H_002685"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFF8366053.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          887 a          551 c          469 g          879 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaa
//
LOCUS       DAXWLB010000219         2622 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXWLB010000219 DAXWLB010000000
VERSION     DAXWLB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.225"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(63..1106)
                     /locus_tag="ACGE4H_002686"
     CDS             complement(63..1106)
                     /locus_tag="ACGE4H_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFF8366054.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1483..2448)
                     /locus_tag="ACGE4H_002687"
     CDS             complement(1483..2448)
                     /locus_tag="ACGE4H_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFF8366055.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT          931 a          482 c          497 g          712 t
ORIGIN      
        1 aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt ttcatgttca
       61 ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag aagcccgaca
      121 gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa gtctaaacgc
      181 agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg tgccgattgg
      241 tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac aaaccctaat
      301 acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt ttcaaacaga
      361 gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac gagcatcata
      421 atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag taagcttgcc
      481 ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact tttttcaatg
      541 agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat cacagtaccc
      601 agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg gatggaaggg
      661 gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat catcaaagaa
      721 gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat agtgactgac
      781 atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt ttgagctgtg
      841 acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc aaattttgtg
      901 ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc caataaaata
      961 gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct gaaggcagcg
     1021 atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc ctttacagat
     1081 tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa atactaaaag
     1141 catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga ggtcgtgaca
     1201 aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc ttctacggaa
     1261 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     1321 gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     1381 gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc gttcttatta
     1441 cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga tttttttaga
     1501 aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat gagcaagaac
     1561 gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat ccaatacgct
     1621 actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg ttgcagagat
     1681 aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga acaaacgtgg
     1741 aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg ctacgacaag
     1801 ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag aaccaggtgc
     1861 tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga aaaagacacg
     1921 tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa agaaaatttt
     1981 tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc caacgtaaat
     2041 gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa catggtgata
     2101 attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa tgaatttgac
     2161 aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga gtgacatcgt
     2221 aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg tgtgttcgaa
     2281 taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac cgccagttag
     2341 attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag ctaacccaag
     2401 gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat ataatattct
     2461 cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa atgaatagga
     2521 atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact agttggatca
     2581 gcaactgttt tgttgtacat ttctattata gatggttttt tg
//
LOCUS       DAXWLB010000220         2572 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXWLB010000220 DAXWLB010000000
VERSION     DAXWLB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.226"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            78..263
                     /locus_tag="ACGE4H_002688"
     CDS             78..263
                     /locus_tag="ACGE4H_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366056.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            768..962
                     /locus_tag="ACGE4H_002689"
     CDS             768..962
                     /locus_tag="ACGE4H_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366057.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1153..1402
                     /locus_tag="ACGE4H_002690"
                     /pseudo
     CDS             1153..1402
                     /locus_tag="ACGE4H_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1646..2476)
                     /locus_tag="ACGE4H_002691"
     CDS             complement(1646..2476)
                     /locus_tag="ACGE4H_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFF8366058.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
BASE COUNT          772 a          448 c          503 g          849 t
ORIGIN      
        1 attttgagtc actttttgtc aatattatta tttacaatac cttctataat aaaagttatt
       61 taaggaagtg tctatgaatg agtaaaaatt ctaaaggatt cctaaccatc ttattagctt
      121 ttatagggta tatgttggtt gggctgctaa aatcatacag taatgagttg ctgaattttt
      181 caacatttat taatgataca ttagttccaa gtttgttttt cattgtattt tttgctgttg
      241 gttattttat aataaagata tgatgactaa tttagctcaa ctattccagc gtcagctgaa
      301 gatattgcaa ttcccgattc cttaatcatt tatctggatg agacagaaag tgaagttgca
      361 acagatgtgc atgtagttga ggataaaaat gatagtttaa aaattaatag ttcagatact
      421 ttactaagag taagtcctat cttttcagct gttatttaga tgcaagtagc aactaagtta
      481 ggtagtactc ctgcaatgga taggtatttg tgtgttcgat atataagtgg tcgaagtatc
      541 aaggttatgt atactggatg ggagatagaa gagttaaaaa ttggaggtca ccgccacaaa
      601 tggaatttag atttagaggt accttagatt tggtatagtt attaaacaca agatggcctg
      661 gtttgtaggg aaagtcgaag agtatttttc cacttctgcg cccctttaat ttttctagta
      721 atgccgtgaa tttgtgatta attggaaata tgaagaaagg agagtctatg aaaacaacac
      781 tagttctatt ctataaaaag catccttatt ttacacttct gataaatata ttgttggcta
      841 gtgttatcgg gatttctgtt gaatatctaa taaataaaga tttcataggt tcttgttttt
      901 atacggcttt atttcttgga cttcttgaag cattttccat ttataaaaaa tcaaaaaaat
      961 aaaatagatt gccgtaagag atctttttca tttcattttt ttctgaaaac ccgttgtttc
     1021 tgtcagtgaa gcagcgggtt tttagattta ttgtctttag acctaattga gcgcgccgag
     1081 cgattgaaat acaaaaccac ttgctatgcg ggtggagcaa taaagggaaa tagtttagcc
     1141 cgtacaaagt agatgtgtaa ataccacatt gtattcacac caaagtatag aagaaaaata
     1201 atttataatc aatatcgagc caatattcag gagatcatca agagattgtg caaatctaaa
     1261 atataaattt gggtatcgac acttttgggt ggaaggatac tatgtgacaa aagtaggaca
     1321 aaatgaagcg accatcaaaa agtacattca agaacaagaa aaacatgata gtgcgttaga
     1381 taagctgagt gtacgagagt aagacgatcc ttttaagggc taaggagtag tacataaacc
     1441 ttttaagagg tgggcaaaac tggcaaaggc aatatggctt gaacataaaa tatgaaagcc
     1501 agcgtcttta ggcgctggcc ggtgatttga gcttatagct ctagagaaaa ccaccctttt
     1561 gaagggtggt tatgattaat gactttagtc agttttgctc acaaagcgtg tggaacaaaa
     1621 aacgggtgtt tctacgggta gatatttatt tcttgaatgg aggtgtccca tacatttttg
     1681 gtgttactgg ttctaatttt ggtgcagcgc cttcgatctt aggttctttc aaccattgat
     1741 attcgccttc gccatccagt gctttgccac tagcccaaga accttcacta ctttggtctc
     1801 cctctgagaa gttatagaag tcataagcaa tgtcttttcg ttctttgtca cgcgggaaag
     1861 tacttgggac taagattgaa ccttcttttt cttctaattc tttcactgca cattcccata
     1921 gattttgatg atagctatct cttgcaagga gaacagacag catatctttt acgccacgat
     1981 catcgatcat ttcgtaaata cgtgctactt gaagtcttcc ttgtgattcg gcatttaaat
     2041 tagctctaaa gtctgctaac aagttgccac tcgcaatgat ataggcaccg ctccatggat
     2101 tgcccacgct gtcagcagga cgaggtccta agccagctac gatcgcgtgc tgtggattca
     2161 ttccagagat aatggcaccg attgttggat ctttttgcat cgctgcttct tgatcttcaa
     2221 caggcgcacc ctctaataaa cgagcaacca ttgtcgccaa catttccacg tggccgattt
     2281 cttctgttgc aatgtccatc agcatatctt tatatttttc ttctcctcgg cagctccagc
     2341 cttggaacag gtactgcata gctacactca tctcgccata ttggccgccg atcagctcct
     2401 gcagatattt cgcgattaat ggattcggac gatctggttt tgcttcaaac tgcaattctt
     2461 tctggtgtct aaacatcaaa attcctccta atctattttt aatacattct cattctgcta
     2521 cgaaagatta aaaaattcaa agaaagcgct cgcgaattat ataaagtatt gg
//
LOCUS       DAXWLB010000221         2571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXWLB010000221 DAXWLB010000000
VERSION     DAXWLB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.227"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(69..971)
                     /locus_tag="ACGE4H_002692"
     CDS             complement(69..971)
                     /locus_tag="ACGE4H_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFF8366059.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(968..1660)
                     /locus_tag="ACGE4H_002693"
     CDS             complement(968..1660)
                     /locus_tag="ACGE4H_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFF8366060.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(1703..2344)
                     /locus_tag="ACGE4H_002694"
     CDS             complement(1703..2344)
                     /locus_tag="ACGE4H_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFF8366061.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(2382..>2571)
                     /locus_tag="ACGE4H_002695"
     CDS             complement(2382..>2571)
                     /locus_tag="ACGE4H_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366062.1"
                     /translation="NFYRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGA
                     VSKMVKSGNVANISYLMI"
BASE COUNT          711 a          573 c          423 g          864 t
ORIGIN      
        1 aaaattctgt cacaacccct cttttttgat caacggtgtt caataaacag cgttctcatc
       61 tgcttttctt agctctgctg gtccagtaac cgagtaagct ctttgataaa tctttgagcg
      121 atttcactga gcggacgttt cgccgaataa atgatataaa attcatttag ttcgccttct
      181 gccaaaggga ttaaccgcaa gtgctcgcga atccctccaa tcacaatacc agatcctgaa
      241 gcgtaagcat cagtttcaag aacgatattc atcaaagtac ctcgatcact cgtataaatc
      301 actgtttctt gatcagggat ctcgataagg tcttcataaa aatacgggaa attatttcca
      361 tcctgtgtaa aacgaacttg aggataagat aacaattctt ctttggtgat ctctgcgtgt
      421 tcagctaatg gatgtccttg ccttaaaaaa atatgcgttt tgaattttcc aatgctgcgg
      481 ccacttagct ctccttgttg taaatatctt ctgataccag cttgattatg ctcattcata
      541 taaataatgc cgatctcact gtgctgctct ttgacttctt cgatcactct cgctgttgtc
      601 gtctccatga tccgaaattg tttgatctcg tctttgaatt cctcgagtag ctgtgcggtc
      661 aaaggaccta agaaatcata atgctgggca gcgatcgaaa aattgtctct cttttcactc
      721 gttagatagt gagtttctaa tgtatcaagc tggaccaata ttttctcggc acgatttaag
      781 aagtcttgcc cttcttctgt taaatatgcc cctcgattcg ttcgagtaaa cagcgtgatc
      841 cctaactcgg tttctaattc tttgatacca gctgaaagac ttggctggga gacaaacatt
      901 ttctttgccg cttctctgaa actaccattg tttgctacag cgaccacgta tttcatctgc
      961 tgaatattca ttgctcaaac tcctcgaaat aataaatttc tcttccattc aagggctgga
     1021 ttggtcggtt gttcggtcga gcatactgac catcgaaggg gagaagaaac tcttggttca
     1081 gttttcctac atgatccatt acaaaccatt ggattggccc gctggtaggc ggagttgtca
     1141 atgagccaat ataatggaaa tggctttttt gctccggaaa gaacacatcc ggatttaccc
     1201 aatgctcatg tttttctaag ttccaacttt ccccattttc ttgattatac agccaacctt
     1261 cctgtaccat tagctggtac atgacaccca caactagatt ctcttttttg ctgttcatat
     1321 gaacaaaatg aaattcaata tcttcctgtt ttccgttgat gatatgttca gaaggcaagt
     1381 ggaaatgaat atctgtcaaa taatagcgtt ctttcttgaa ctctacataa ctgagttggt
     1441 caaaaggtac taaatgaatc gtgtttttga attccttgtc agtaaaccgt tgcttttgat
     1501 aatgaaagga aagcgtgtct ccttctattt tggtttcttc ccccttggtc aaatgaatag
     1561 gcgactgcaa tggaaattcc gcacctctcg caaaccaatc acataaactc gcccaatttt
     1621 ccggaccttt gtctccagaa tagccccatt cgacatccat attctttttc aactatactt
     1681 ccccctgcat ctcatatcat ttttattttt cttctttctt tacaatcgtt gagacggttt
     1741 ctaaaaacgc ctcttttcca taaaattcct ggttcaatag ttccggaaat atctctttaa
     1801 agaaatactg aacagaaggc cgatgttcaa agcttttaat cgtagtcact gcctcaatga
     1861 acatctcctt ataaactttt tccgggttat ttttgatgat ttcatcaaaa gattcataaa
     1921 gaaggtcaac cttatctgaa atggctaata gttctccttc tacggtttca tcttttcctt
     1981 cgaacaaccg acggcgataa acttcttgga actcagctgg aatctcttgg ttgatgaatt
     2041 cgtccgtcat cttttcttca actgtttgca gcatccctct taactctttg cttgcgtatt
     2101 tcaccggcgt tttgatatca ccaataaaac gctctgtgta gtcatgattc aatgattttt
     2161 catagagcag tttccaatcg atcggttgtc cattcgcttc ttcaatgtct cctagaactt
     2221 gcgcaataga tgctacacga tacgaatgtg ctgcaactgt atgctctgta tatttaaaaa
     2281 atccaggtgc ccttgtgatc gtctctaaat tgttcaatcc taaaataaat tcattcaacc
     2341 ccataaaagt tacctccctt tgagatttct ttttccgaat atcatatcat taaataactg
     2401 atattcgcaa catttccgct ctttaccatt tttgaaacag ctcccttacc aaaaaataga
     2461 gaaaatctat atataataga aaaaagagaa gagattattc tcttctttct gctaaccagt
     2521 cttagaaaaa tgaattttaa aaaaatcaaa aactgatcac gatagaagtt t
//
LOCUS       DAXWLB010000222         2563 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXWLB010000222 DAXWLB010000000
VERSION     DAXWLB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.228"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            48..659
                     /locus_tag="ACGE4H_002696"
     CDS             48..659
                     /locus_tag="ACGE4H_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFF8366063.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            732..1661
                     /locus_tag="ACGE4H_002697"
     CDS             732..1661
                     /locus_tag="ACGE4H_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFF8366064.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1798..2556
                     /locus_tag="ACGE4H_002698"
                     /pseudo
     CDS             1798..2556
                     /locus_tag="ACGE4H_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          894 a          358 c          478 g          833 t
ORIGIN      
        1 ttatatgaag ttataaaatt taatttatat tggaaggagg aaaataaatg tattttatac
       61 tacctacact ttatagcctt ttatgtgtaa tggcaccatg tattatttat atggtatttt
      121 tttgcaaaaa aaataccctg aaacacaata tcctgatttt gatttttatg ctgtactgtt
      181 ggaaagtttt tgaattgact ggtacaggtg gactaacaga tattttttat agacctgata
      241 caggtaggaa attttctcta gtacagggca gtgttaatct tgtaccattc agtggactaa
      301 acattagctt tatattaaat atagtaatgt gtgtgccctt aggattctta ctaccaatca
      361 tatggaaaag ttataggaaa atagttcctg ttttcttggc tggggctcta ttttcactaa
      421 ttattgaagt ctcacaaatt ataactacaa gagcaacaga tattgatgat ttaatagcaa
      481 atacctttgg tgcggttata ggctttatac tgtgggctgt tttaagtaga gtggtgtcct
      541 tgaaggacaa gtcatctaca gtagattatt tagaacctta tatttatata ttgattggtt
      601 ttaccggaaa attcttactg tattttcctt tctggtttgc ttttgaagta ttatcctgat
      661 aattccatag aaaataatga gtttgagttg agaaagaata ttcttgtaca caactctgga
      721 aaggaggaag tatgaaaaga ttcttaaaat gggggcttgt tcttttagcg tctttgattg
      781 tgattctaat gtgtacagcg gttgttattt cttatgtgaa tcggccgtat gcaagagctg
      841 tttatgatag gattaaacgc attcttgaca aagattttta tgaccatcta tatctaggtt
      901 cttcatttga aataataaac cttgatacaa tggatattca tagctatatg aaaaaagacc
      961 aagaagaatt acccgcctca ttgagcaaat tatttacgat tgattatgcg cttagtgtcc
     1021 taagtttaga tgaacagatt acaattagtc ctactgcact tgacatgcag aaagctgact
     1081 catctactgc acatcttcaa gaaggagata ttttatctgt ttatgacatt atctgtgcta
     1141 tggtcttacc ctcaggaaat gatgcagctt atagtttggc aatttataca ggtggaaaac
     1201 ttgatcctga tgcaaagaat gatgaagaac gctatcaaag cttcatgaaa aacttagatc
     1261 gttatctaag tgagcaaggc tataaacata ctcattttac tgacgctgct ggctatgact
     1321 ccaatgctac aacatcagcg gaagatttga caaaagtagc tgcaaagcta ctaaagaatg
     1381 ataaatttgc agaaatagtt gccaaggaaa cttatagagc aaaatcaatt aagggaaatg
     1441 tttataactg gaatactacg aatcaattta tgcaacaaga taatagatac tttactgaag
     1501 gtgtaaaagg tgtaaaaact ggatcgtttg atgggagata caatctatgt gtgtattata
     1561 catctagttc tgataaccac tatttacttg taagttttgg ttcagagagt gatagtaggc
     1621 gttatgtaga tgttcaaaat ctattgcata atctgccttg aaatttcgtg cctttaagcg
     1681 cgtatctaaa gtttctttga aaagtttgag aatgaagaaa gtgagcagtc gaaaaacggt
     1741 tgctcacttt tttgccctaa aatcgaaaaa atataaaaaa gtcctttaca acacgtctca
     1801 ggaaaaactg ctaagtcaat tctaatatct tgtggagcaa gacttgcacc ttttgatata
     1861 agagagctta gagaactaat gagcgaagat gaactagaac tcgataaaat tggagataga
     1921 aaaactgcct tgtttgtaat aatatcagat acagatgata catttaattt tgtagtttca
     1981 ataatgtatt ctcaattatt taatctacta tgtgataagg cagatgatgt gtatggtgga
     2041 agacttccag ttcatgttag gtgtctactt gatgagtttg caaatatcgg tttgattcct
     2101 aaatttgaaa aactaatagc gacaattcgt tcaagagaaa tatcggcaag tataatactg
     2161 caagcacaat ctcaattaaa agcaatttat aaagaccatg cagatacaat agttggtaac
     2221 tgtgactcta cactttttct aggaggaaaa gaaaaaacaa cagtaaaaga attatcagaa
     2281 accttaggaa aagaaaccat agacctatat aacacatcag aaacaagatc aaatcaaaaa
     2341 tcttttggtc taaattatca aaagactggc aaggaactta tgagtcaaga tgaaataact
     2401 gtaatggatg gtggtaagtg tatttaccag ctaagaggag caagaccttt tctatctgat
     2461 aaatttgata ttacaaagca taagaattac aaattgttgg aagattatga taagaaaaac
     2521 ttgtttgatg tagaagagta tttaaaacaa agatagcgta aaa
//
LOCUS       DAXWLB010000223         2533 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXWLB010000223 DAXWLB010000000
VERSION     DAXWLB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.229"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..331
                     /locus_tag="ACGE4H_002699"
     CDS             <1..331
                     /locus_tag="ACGE4H_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8366065.1"
                     /translation="ESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILA
                     DEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVN
                     KMNLEVE"
     gene            331..1707
                     /locus_tag="ACGE4H_002700"
     CDS             331..1707
                     /locus_tag="ACGE4H_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8366066.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            1837..2250
                     /locus_tag="ACGE4H_002701"
     CDS             1837..2250
                     /locus_tag="ACGE4H_002701"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366067.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(2275..2533)
                     /locus_tag="ACGE4H_002702"
                     /pseudo
     CDS             complement(2275..2533)
                     /locus_tag="ACGE4H_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          933 a          353 c          458 g          789 t
ORIGIN      
        1 tgaaagtatc ttgtttgaac taggtttaga taaaaaacaa ataaaaagaa atgttatgaa
       61 attatctggt ggacagcaac aaagagtagc tattgctaga gcattggtat cagatgctcc
      121 aataatacta gctgatgagc ctactggtaa tctagacagt gttactgctg gagaaataat
      181 taatatatta aagacattag ctaaagatag aaataaatgt gtaatagttg taacacatag
      241 taaggaagta gcagattctg cggatatcat tttagaacta agtggtaaaa agttgaaaaa
      301 agtaaataaa atgaatttgg aggttgaata atgataaaaa atgcatttgc ttatgtaact
      361 agaaaaagtt taaaatcatt aattatcata ttagttattt tatctatgtc aactttaagt
      421 ctcattagtt tatctattaa agatgctacg gacagagctt caaaagaaac atttgctaat
      481 ataacaaata gtttttctat ggagataaat agacaagtaa atccaggaac acctagaggt
      541 gggggaaatg taaaaggtga agatattaaa aagatatctc aaacagatag tatagactcc
      601 tatgtaaaaa gaataaacag tgttgcagat ttagttgatt atgatattat tgaaactcaa
      661 gagactcttg cgaatcaatc acctgaaaga gcaaagaatt ttaaaagaac agttatgtta
      721 actggggtta acgactcaac aaaagaaaca aaatttgtat cagaagcata taaattagta
      781 gaaggaaagc atttagaaaa tgaagataaa aataaaatct taatgcataa agatttagct
      841 gaaaaaaata accttaaagt tggagataag ataaaaataa aatctaattt atttgatgct
      901 gataatgaga aaggtgcaga tgaaacagta gaagtagaaa ttaaaggtct attcgatggg
      961 cataatagcg gtgtagtaag tgcagcacaa gaactatacg aaaatacatt aatcactgat
     1021 attcatacag ctgctaaagt ttatggtaat accgaagata cagctgtata ccaagatgca
     1081 acattctttg taaaaggtga taagaatctt gatagtgtaa taaaagatct tgggaaatta
     1141 gatataaatt ggagagaata taatttgatt aaaagttcat caaattaccc tgcattacaa
     1201 caatctatat caggtattta ctcaatttca aataaattat tttttggctc attaatattt
     1261 gcaggagttg tagtttcatt attattattc ttatggatga atgctagaaa gaaagaaata
     1321 gcagtattac tatcattagg tatatcaaaa ttagaaattt ttggtcaatt cctaattgag
     1381 atggtattta tatcaatccc cgcattcgtt ggttcctatt tcttagctca atatacagct
     1441 gacaagcttg ggaataatat attgaataag gttactggtg atatagctaa acaaatagct
     1501 aaacaatctg catctagcca attaggtggt ggggcagaag ctgaaggatt taacaagaca
     1561 ttatcaagtt tagatatcaa tgtattacct aaattcatga tttatgtagt tatatttatg
     1621 agtttagtac ttcttgtatc attgattatt tcttcattca atatattaag aaggaatcca
     1681 aaagaattat taattgacaa taattaaaat tttcggtgct ttttagcacc aaaaatgaga
     1741 gtgtaatata aaccgtgtaa gtaaccatca atcctaggcg ggtggttgct tcacggtttt
     1801 tatttttcta aaataagatc aagaggtgat tacacgatgg ctagaaagaa aagaaatcct
     1861 gatgctgaga agttagctga atccattttg aatacttatc aacccgaatc tgtagaggat
     1921 atgcaagatg ttttaaaaga tgtgtttggt cccttatttg aaaaaatgct tcaaggagaa
     1981 ttgaataacc atttagggta tgacgcccat tctaaagagc caaagaaaca tgacaatcga
     2041 cgaaacggtt atggcaataa gacgcttaaa acgagctttg gtaaagtaaa cattgatgtt
     2101 cccagagacc gggaggcttc ttttgaacct gagttgattc ctaatagaaa acgagatgtc
     2161 tccgacatcg cagactgtag acaaaagctg ttccaaactc gtaactcgag cttggaacag
     2221 ctttttatct acttcgtttc gaatttttga acaaaaaaat aaaggctcct tgcctttcca
     2281 tagccaggtg gctaatttct ttaaattcat tgcagcaaac actagcatcg tatgcgtcgc
     2341 gactttttcc aagcctcgat acttggtcca acgcatccca tgcttttctt ttgcatccgc
     2401 aaatattcgt tcaatcgttt gtttccgttt tttgtaaatt gatttattca gatctgttag
     2461 gcgaagatgt tccacttcat ccatcagatc tccccaaaca tgtctcgtga tgactcgttg
     2521 atgattttta ctt
//
LOCUS       DAXWLB010000224         2475 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXWLB010000224 DAXWLB010000000
VERSION     DAXWLB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.230"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..418
                     /locus_tag="ACGE4H_002703"
     CDS             <1..418
                     /locus_tag="ACGE4H_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366068.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE4H_002704"
     CDS             632..1327
                     /gene="vanR-A"
                     /locus_tag="ACGE4H_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8366069.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1305..2459
                     /locus_tag="ACGE4H_002705"
     CDS             1305..2459
                     /locus_tag="ACGE4H_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8366070.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          854 a          443 c          521 g          657 t
ORIGIN      
        1 acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta gtacacaaga
       61 tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat cactaaaaga
      121 tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta ctgtaatggc
      181 tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag ggattgaatt
      241 ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa atcacgcagg
      301 aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc aaatttgtga
      361 aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga ataattagcc
      421 attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta taaaatatta
      481 acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca tcttaagaaa
      541 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      601 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      661 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      721 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      781 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      841 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      901 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      961 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
     1021 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
     1081 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
     1141 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
     1201 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
     1261 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
     1321 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
     1381 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
     1441 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1501 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1561 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1621 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1681 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1741 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1801 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1861 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1921 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1981 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     2041 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     2101 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     2161 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     2221 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     2281 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     2341 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     2401 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     2461 agatgtatat aattt
//
LOCUS       DAXWLB010000225         2413 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXWLB010000225 DAXWLB010000000
VERSION     DAXWLB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.231"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..337
                     /locus_tag="ACGE4H_002706"
     CDS             <1..337
                     /locus_tag="ACGE4H_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366071.1"
                     /translation="YPTETVNQAIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYD
                     NYERVDLVSTLPELMFVICVYGKTQKEVKNHADVMKQAGGRELDLQRITEGDLINVIT
                     KLMNPNTSL"
     gene            354..821
                     /locus_tag="ACGE4H_002707"
     CDS             354..821
                     /locus_tag="ACGE4H_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366072.1"
                     /translation="MLKHCRKGFCKVIKGKNSADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEENSNSKSAFFSEKNAFLHSI"
     gene            837..>2413
                     /locus_tag="ACGE4H_002708"
     CDS             837..>2413
                     /locus_tag="ACGE4H_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFF8366073.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECH"
BASE COUNT          890 a          311 c          477 g          735 t
ORIGIN      
        1 ttatcctact gaaacagtaa atcaagcgat ttattggaag catagaatac aggaggcaaa
       61 gagaagttta ttgagtaata atgatcccaa tttagaaacg tatctgaagt ggaatctaaa
      121 gctagcttac gataattatg aaagagttga tctagtatcg actttaccag agttgatgtt
      181 cgttatttgt gtttatggaa aaacacaaaa agaagtgaaa aatcatgccg atgtaatgaa
      241 acaggccggt ggtagagaat tagatttaca aagaattact gaaggggatt taatcaatgt
      301 gataactaaa ttgatgaatc ccaatacttc tttataaggc ggtgcttatt attatgttaa
      361 agcactgtag aaaaggattt tgtaaagtaa ttaaaggtaa aaattctgct gatttaatct
      421 ttgaatggaa agaatctaat ttaattaatg tacgtgggaa aaatggaagc ggaaagacag
      481 aaagagtgat ggctagtatt gataattcaa ggcctagatc aagagcatta gtaataactg
      541 atgaaaaaaa tcactataaa ggcatacgaa cgatagaaaa aaaatattta actaacaatg
      601 atttaaaagt agtagatgat attttaaata tttatcctga ttttaattta gttgtttttg
      661 atgaaacaat tcctgtgaca gaaataaaag gattctatca aagcttttcc aatctagcaa
      721 ataaatatga ggaagttctg tttgtttgga ttacagaaga aaatagtaat agcaagtcag
      781 cgttctttag tgaaaagaac gcttttttac attctattta aaaaggagaa ttgaacatga
      841 ttgtaagtaa aaaattagca agaaatctag taagtgaagg gtatgactta agattagtag
      901 aaaagtatca accgagcggt ggaattaaat ttgatgagcg ttatatttct actggtgatg
      961 gatatgtaac ttcgattgct gttattgagt ttgcagaaga tgtaaatagt cgatggttaa
     1021 cggatataat ggcaattccc aatactattg caactttcga tgtatgtacg gcggataaag
     1081 aagacttatt acacaaaatc aatacgtcta ttggagaatt agattaccga tcaacaaatg
     1141 agaatagaac aactgacaaa acagacgctt tactagataa atacaattta gaagattttg
     1201 cacaagaaat tacgaaacgt ggtgaagttg tcaaaactgt tcatgctcgt atttttgttt
     1261 atagcgattc tttagaagaa ttagagaaaa gaattagtga gattcgtaaa gatttggcta
     1321 gtgtcaatca caaggcaatt gttccgcaat ttagaatgaa ggatgaatgg atgtctttat
     1381 ttcaagatgg catggaacaa gaatcaaaat tatttgccaa gaaaggctta actgttccgg
     1441 caaaaaatat tggtggcggt attccatttc accacaagag tttgaaagat ccacgtgggt
     1501 tttatttagg cactacgtca actggtggtg cttttatatt tgatccattt tattctacga
     1561 agcaaagacg gtcgtttaac ggctttattt taggaaagat gggttttggt aagtcaactt
     1621 taattaaaca gctacaggaa ggcctacaag cgaagaattg ttttattcga ggatttgaca
     1681 agtcgggtga atttgaaggc atggtgaaga aagcaggcgg tgtatacgtc gatttaggcg
     1741 gtggtgatat ggtagtaaat ccattgcaaa tttatgcaac agtcatgaaa aaagatcaat
     1801 ttggaaatga tgttgtagat gaatcaaaga gttttcaaaa tcatatcact aagtgcagta
     1861 atcaaattcg ctttttagaa cctgaaattg atacggcaac tgtacgtgaa tatactttga
     1921 tgctacaaga cttttatatt cattatggaa ttttgccaaa agaatggaga gaagcgagag
     1981 acgacaacga acgaaatcaa ttaaatacaa atatttgtaa cttgcctaat gattcatatc
     2041 caatctatgg agagtttctt aaatttattg aaaatcaaca attcaaaatt aaaaatgcta
     2101 ctcctgaacg tgtccgttct attgagctaa ttaagcttat cgttacggat atgtgtacaa
     2161 cttatccgca aattttcaac ggagtaacaa caatgccaaa ctttgataca acaaaaattg
     2221 tttactataa cattgattct attagtaaaa tgagtgcccc agtattccat tgccaattat
     2281 ttacagcgtt aacgactatt tggaacagtg ccttgcaaaa cggaaagatt caaaaagaaa
     2341 actttaaaaa atatggcagt gattattata agtatgcaca agatttcatg gttttgatcg
     2401 atgagtgtca caa
//
LOCUS       DAXWLB010000226         2403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXWLB010000226 DAXWLB010000000
VERSION     DAXWLB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.232"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            26..238
                     /locus_tag="ACGE4H_002709"
     CDS             26..238
                     /locus_tag="ACGE4H_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366074.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(516..2315)
                     /locus_tag="ACGE4H_002710"
     CDS             complement(516..2315)
                     /locus_tag="ACGE4H_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFF8366075.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          840 a          440 c          450 g          673 t
ORIGIN      
        1 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
       61 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
      121 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
      181 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct actgaattgg tttcttaaac
      241 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
      301 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
      361 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
      421 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
      481 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
      541 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
      601 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catggcatct
      661 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
      721 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
      781 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
      841 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
      901 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
      961 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
     1021 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
     1081 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
     1141 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
     1201 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
     1261 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
     1321 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
     1381 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
     1441 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
     1501 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
     1561 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
     1621 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
     1681 aatgaccagc tttcccgaat ggctgctcga aaagaacgat ctcgtagaat catttccggt
     1741 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
     1801 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
     1861 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
     1921 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
     1981 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
     2041 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
     2101 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
     2161 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
     2221 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
     2281 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattgta
     2341 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
     2401 aac
//
LOCUS       DAXWLB010000227         2392 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXWLB010000227 DAXWLB010000000
VERSION     DAXWLB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.233"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(326..523)
                     /locus_tag="ACGE4H_002711"
     CDS             complement(326..523)
                     /locus_tag="ACGE4H_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFF8366076.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
     gene            complement(878..1963)
                     /gene="xerS"
                     /locus_tag="ACGE4H_002712"
     CDS             complement(878..1963)
                     /gene="xerS"
                     /locus_tag="ACGE4H_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFF8366077.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            2312..2392
                     /locus_tag="ACGE4H_002713"
                     /pseudo
     CDS             2312..2392
                     /locus_tag="ACGE4H_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
BASE COUNT          772 a          357 c          378 g          885 t
ORIGIN      
        1 ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttcattgt gatccacctc
       61 ttttgtttat tagtttaaac catcaaacca tttttaataa aatatgtaaa ttcctttgct
      121 caaaaaagta gcttctttac gattcgacaa tttcaaataa agcaggtaaa aactggctgt
      181 cagaaaatag ccaaactgaa gcaaaaaata atgttaaaat taattaatga acgaaaaaaa
      241 tccctcacct aatattacgg tgaggaattt tttctaccga cataaacaaa aaaaccacct
      301 catatgaggc ggttaacgtt caacattatt ttacgatatt tactgcttga ggaccacgtt
      361 gtccttcttc aatatcataa gtaactgctt ggccttcatc taaagactta aagccttctc
      421 cttggattgc tgagaaatga gcgaatacgt catttccatc ttcaccagtg atgaaaccaa
      481 aacctttatc tgagttaaac cattttactg taccgttatt catatatatt tcctcctgat
      541 acgtatataa tacgtgtttt attgcaatta atattacttg gtaaaaggag tttttatagt
      601 gtgaatatat cgctcagatt acgtttcaaa ttagattact tatttactat aacacggatg
      661 taatgctaac acaaggataa tcactcgata tacggtttaa atattattat ttgtcaaatt
      721 aagcgagaca aaactttcca tttacgtaat tcaaaaaaac ttctaatggt gtttggtaat
      781 ttagtaatat aaaaatgata atgaagcttg cctttaaagt tgggctttta tctaatttta
      841 gagacaggct tcattattat actatttttt actacattta caaagcgttc aaagcatcaa
      901 ttgtagctgc attgtctata tgagtatata aagttgttgt atttgtactt gtatgtccca
      961 attgttgggc agttaatacc aatgaatttg tctgttgata gagtctagta gccaaagtat
     1021 gacgtaattt atgtggactg acttggactc tgaatccttt tgaatatttt gctaccatcc
     1081 gttcgattgc aataccggat atacgcttgg cttctccttg ataaattgct agaaataaag
     1141 ctttttcgtt ttctgttact ttgtatcgtt ctttccgaat agctagataa ttagctaaat
     1201 attccattgc aaatgctgct atatttactg cgtctctctt atttcctttt ctagttacta
     1261 ccacattatt cttttctaaa gataggtcct ctaaattaat atttacaacc tcggagagtc
     1321 ttaatccaga tcctaaaatc aatgcattga tcgccaggtc tctttctaca tctcgttgat
     1381 aatagatcaa agctcgtttg ctcaaagttt ttttatattc attggcaata taatctagat
     1441 aattttgtgt atcttcgtct agaaataatt ttttctcgat tgcgtttgca cgggcagcta
     1501 acgtagtttt atctgtgata gttgctactt gatccatagg attattttct aagtatggtt
     1561 ttcctaaagg attattggaa cttttcgaaa gatatttaaa tagaaccttt aatgctgtga
     1621 tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg agcttagttt
     1681 ttgttgtctc tttttttaca ttttcctttg tgcgacgttt caaatgagat ttgtacaagt
     1741 ttagatcata ggcagttaac tgtcttatcg aggtgattga agtatcggga acgatagact
     1801 cactcagaag ccattcataa aagagcttat aattttttag atattctaaa gtagttcgag
     1861 gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg ggcatatgaa
     1921 gtatgattgt atctattttt tgatatattt ctgtattttt catgttattc atcctttcga
     1981 taagtagggg agagaagtat ctctataatt ggactatacc aattatagaa tgttttgtca
     2041 attggtatac tctgaaattt attaatttat ttcaaaaaag cagataaata ggtatagatt
     2101 taataattta aaataaatgg tatacatgaa taggaaagga aacagaagga ggcaatctta
     2161 attttttaaa ttgtctaaat tatatagaaa gaacaatatt tctatataat tagacataag
     2221 agagactacg gttgatttga tattaacaag cgtatatgaa tgtatataca aataaaaaag
     2281 gggagggaga agttaaagaa aattcagaga agtggggcac tcagtagtat taacaattta
     2341 aaaggaattt cttaaagaaa atgggttgca tcctggagat acaatttttt ta
//
LOCUS       DAXWLB010000228         2389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXWLB010000228 DAXWLB010000000
VERSION     DAXWLB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.234"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..209
                     /locus_tag="ACGE4H_002714"
     CDS             <1..209
                     /locus_tag="ACGE4H_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366078.1"
                     /translation="AATILCCGLIFSQLSIYPRYQRLFIHFLNIQCASMPLEVIKKLL
                     INPIFMVSLVLLTGLVTWVLGKKE"
     gene            252..2351
                     /locus_tag="ACGE4H_002715"
     CDS             252..2351
                     /locus_tag="ACGE4H_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFF8366079.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
BASE COUNT          867 a          367 c          466 g          689 t
ORIGIN      
        1 tagccgcaac aatattgtgt tgcggtttaa ttttttctca attatcaatt tatccaagat
       61 atcaacgatt gttcattcat tttctaaaca ttcaatgtgc ttccatgcca ttagaggtga
      121 ttaaaaagct cttgatcaat ccaattttta tggttagttt ggttcttctt acaggattgg
      181 taacgtgggt attaggaaaa aaagaatgaa aagcttacga tcgtttaaaa aaatattaga
      241 tggagggcat tatggaaaat aaaattcatt gcactacggg aagagtaatt actttagtgt
      301 tgtttttgtt ccttcttctt tttgcgaggg gaactgcagt ccaggcctta gaaaaagttg
      361 atccgagtca aatcaaagaa aataataatc tggtgcttta tcatgatagt gattcattga
      421 gaaatatcga agtaactggg atcaatggat ttaagatact agatgagtat tcaactaaaa
      481 aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa aagtacgtca aaaatcgttg
      541 cccattatca aaaagtaggt acttatctag gaaaagaaat tgattgtgac gtgacttttt
      601 ctaattttgt agcgcacgct ggggttaacc aagacggatt agctaaaaca attaatggca
      661 aaaagtatta taattatttg ctctttgata aaaatattta tcgaggattt ttcatgcagt
      721 caatcaatgt catgaatgca aaactagaat ttaagtatgc aggaacaaaa acaaaagtag
      781 ctttgagtga accacagcca gaaattcaat caaaaatgag atcgatgaga gcagctggta
      841 ttgatttaaa cgatacattt ctatccattt acagtttaaa tggacagtat cagcaacgtg
      901 tgtcttggta taatgacaat aatgaatctg tcggtgaacg taatattgat atgagagaat
      961 ttgttgggta tgaaaaaatg ggtagcttac cttattttgt cacaacagat acagcatgtg
     1021 cagaatacaa agctcctgcg ttatcaacaa acaatttaac ttcaaaagta gtgggaggac
     1081 gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga tgttgtagga gctgatactt
     1141 atcacagaag tggtgtaacg tatacgcttc aaggcgcttc cccaacattc atgattggcg
     1201 caaatacgaa tagtatgatg tttagctttg atactgcatt gctatggaca ccacaaccat
     1261 cgaagcctac aaaagaagtg tttaacaaag ctaatactga agaggcagca cacaatattg
     1321 acaaaaaagt gattccacaa ggatcagatg tttactatca tattcaccaa aagtttgatg
     1381 cattaacagt caacacaatg aacaaataca aatcatttaa aatcactgat acctttgaca
     1441 gcaaaaattt tgatatggta tcggatggga aaaactatga tggcgcattg tggtatgttg
     1501 aagcgaatgg tacttggaaa aaagctgata caggaggaaa gttaactgta tcaggtaata
     1561 cagtcactta tactgcttct gacagctttt tagctaacgc tgcaaattac ggtagggatt
     1621 atattttgca gctccatatg agagcaaaaa atgatgcaaa aggaattgca ccaaatgtgg
     1681 ctactgtaac gtttaacaat gatccgaaca aaggaacaca aaaaacaaat acagtagaaa
     1741 actatttctc tcttaaacca aataaaaaag tggaacaaga tgggaaagat gttgatggtc
     1801 gaaatgatgg taaaaaagga acgcctacag ctcctttaaa tgcaggatct gaagtacaat
     1861 atctagtcac acaaaaatgg catacaaaag gtgtagacat tgcctcagat cattacaagc
     1921 aatttagtat tcaagatcca gtggaagatc gtttgacgta taaagaaggt tctgcgcgag
     1981 tgatagatat gagtacagga aaagatatta cttcagaagg tacactaacc tatgatagca
     2041 attcaagaac gctgaagtgg gaagcttccg ctgatttctt aagtaaaaat cttttagatg
     2101 gacgagaaat tcagctgata tttacagcta aaactccttt gaatgaagaa ttggatatcg
     2161 acaaccaagc aaaagtttca gtagaaagta ttgagaaagc aactaatgta gtaacgattg
     2221 gggtaaaacc taacttacct cagatcattg tgccaaaaac tggatcgaat catttagtgc
     2281 taactgttat tggaagtata ttatttctta ctttagcatc attgggctat gtagttttaa
     2341 aactcaaata aatataaaaa caaggctgag aatcagcctt gttttcttt
//
LOCUS       DAXWLB010000229         2335 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXWLB010000229 DAXWLB010000000
VERSION     DAXWLB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.235"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(51..1598)
                     /locus_tag="ACGE4H_002716"
     CDS             complement(51..1598)
                     /locus_tag="ACGE4H_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFF8366080.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACGE4H_002717"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACGE4H_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFF8366081.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACGE4H_002718"
     CDS             complement(2043..2237)
                     /locus_tag="ACGE4H_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366082.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          653 a          484 c          420 g          778 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaact tatacataga agacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttaaaaatgt tttgc
//
LOCUS       DAXWLB010000230         2302 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXWLB010000230 DAXWLB010000000
VERSION     DAXWLB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.236"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..665
                     /locus_tag="ACGE4H_002719"
     CDS             <1..665
                     /locus_tag="ACGE4H_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366083.1"
                     /translation="LLKDVDKKYYILTLLCALFSIFNVYNLMYDQSWHWNTNDPTAAG
                     NNKSSMVEKDPQKLREAFYNKDLNIALKAIQKGTPDYLPVQKNVEPSDVLKQNPYELY
                     TNQIINNNVHFKKTVTSDSKLRLTWTNNSNEESDIQLPIIIYNHSTVTLNGKKLTPNE
                     IKTTQIGAAIVTSSPGKNTLVIGYKPFVLFKIAFPIKILSILSTIIYVIYKYKKTKII
                     EI"
     gene            791..1933
                     /locus_tag="ACGE4H_002720"
     CDS             791..1933
                     /locus_tag="ACGE4H_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8366084.1"
                     /translation="MAKFEQYKKKNGDKAWKFQAYLGINPETGKPVKTTRRNFKTQRE
                     AKLALARLQSEYEDNLLTKEKPKTYKDVYDLWMTEYKRTVRGSTLLKTERIFKNHVLE
                     ELGDIYISEITPIKIQKLMDKWANKYDTAPKMMNYTGLVFKYAVRFGMIESNPTDAIR
                     KPKRRKKATVEEPFYDKKQLKLFLDELYNQPNLKIQAFFRLLAMTGMRKQEAGALEWR
                     DIDFKAKTVNIYKAVTRTANGLEIDTTKTVGSSRIISIDQGTLDKLLEWKKVALPPSD
                     EWLIFGHSSAKKPHDIMSLDTSRKWLLSIQDKMDKKQKKKLPRITVHGFRHTQASLLI
                     EMGASLKEVQFRLGHEDIQTTMNTYAHVSKLAKEQLADKFNKFIDF"
BASE COUNT          915 a          350 c          363 g          674 t
ORIGIN      
        1 aactacttaa agatgttgac aaaaagtact atatattaac tttactgtgt gctctatttt
       61 caatatttaa tgtatataat cttatgtatg accaatcttg gcactggaat acaaatgatc
      121 ccaccgctgc aggaaacaat aaaagttcta tggttgaaaa agatcctcaa aagttaaggg
      181 aagcctttta taataaagat ctgaatattg ccttgaaagc aatccaaaaa ggaactcctg
      241 actatttacc tgtacaaaaa aatgtagaac catcagatgt attaaaacaa aacccttatg
      301 aattgtatac aaaccaaata ataaataaca atgtacattt taaaaagact gtaactagtg
      361 attctaaact tcgtttaact tggactaata attcaaatga ggaatccgat attcaattgc
      421 ctataataat ttataatcat agtacagtaa ctttgaatgg taaaaaatta actccaaacg
      481 aaattaaaac tactcaaatt ggagctgcta ttgttacatc gagtccagga aaaaatactt
      541 tagttatcgg ttataagcca tttgttttat ttaaaatagc ctttccaata aaaatattgt
      601 ctatactttc aactatcatt tatgtcatct acaagtataa aaaaacaaaa ataatcgaaa
      661 tataaaaatt acaaaacatt aatatgttat gtgatgcaca tccatttaat aacagcagaa
      721 tattataatg gatgtgtaaa aatcactgag attttttagt cattattacc agaaaagtga
      781 gttgattcaa ttggctaaat ttgaacaata taagaaaaag aacggggata aagcttggaa
      841 gttccaagca tatttaggaa tcaaccccga aacaggaaag cctgttaaaa ctactcggcg
      901 aaattttaaa actcaacgtg aagcaaaatt agcactcgca agattgcaaa gtgaatacga
      961 ggacaattta ttaacaaaag aaaaaccaaa aacatataaa gacgtatatg atttatggat
     1021 gactgaatac aaaagaacag tacgaggatc tacattgtta aaaacagaaa gaatttttaa
     1081 aaatcatgta ttagaagaac tcggcgacat atatatttct gaaatcacgc ctatcaaaat
     1141 tcaaaaatta atggataaat gggcaaataa atatgataca gctcctaaaa tgatgaatta
     1201 cacaggacta gtttttaaat acgccgttcg atttggaatg attgagtcca atcctacaga
     1261 tgccatacgc aaaccaaaga gaaggaaaaa agcaactgtt gaagaaccat tctatgataa
     1321 aaaacaattg aaattgtttc ttgatgaact atataatcaa ccaaacctaa agattcaagc
     1381 tttttttaga ttactagcta tgactggtat gcgaaaacaa gaagcaggcg ctcttgagtg
     1441 gagagatata gatttcaagg ctaaaacagt caatatctat aaagccgtta ctagaactgc
     1501 aaatggacta gaaattgaca caactaaaac ggttgggtct agccgaataa tttcaatcga
     1561 tcaaggtact ttagataagc ttcttgaatg gaaaaaagtt gcccttcctc catctgatga
     1621 atggctcatt tttggacatt caagtgcaaa aaaaccacat gatataatga gtcttgatac
     1681 ctctcgaaag tggcttttga gtatacaaga caaaatggac aagaagcaaa agaaaaaact
     1741 acctagaatt actgtacatg gatttagaca tactcaagca agcttgttga tcgaaatggg
     1801 agcatcgctt aaagaagtac agtttcgttt aggtcacgag gatattcaaa ctactatgaa
     1861 cacgtacgct catgtatcaa aacttgctaa agaacaatta gcagataagt tcaataaatt
     1921 tatagatttc tagctatgtg gcattcaaaa tggcattcaa ttaaatttga gcattgatat
     1981 aatagtattt aagcaactcc cgccgtctcc atatatgtat ggttgagaca gataaacatg
     2041 tatgaaaaac gttgttaaat caacgttttt ttattttggt caaggagaga aaagtagaga
     2101 aatacaaaat tttttgcatt gattttgcat tcaaaaaaac attcaaacat ttagaaatcc
     2161 ccaatacgta ttcaggaaaa tctattttaa ggtttacaaa aagaactttt gttcgtacac
     2221 tctttttgag gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaagcatg
     2281 ttccttcaaa taagtttttt ta
//
LOCUS       DAXWLB010000231         2281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXWLB010000231 DAXWLB010000000
VERSION     DAXWLB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.237"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..421
                     /locus_tag="ACGE4H_002721"
     CDS             <1..421
                     /locus_tag="ACGE4H_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFF8366085.1"
                     /translation="LISGKYDYAFDFEGEKDYKSVAVFQSDFEKLKNYAGRRNVNFAR
                     LLEEFYSEELGVGNEYKRETYAKLSHKLSQALKEVTEVKAELDAKLEEVKKYQTERKN
                     NMKNQVEFDEQKEFEKARKRYNKIMNELSYEHLTVHE"
     gene            422..625
                     /locus_tag="ACGE4H_002722"
     CDS             422..625
                     /locus_tag="ACGE4H_002722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366086.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            645..2273
                     /locus_tag="ACGE4H_002723"
     CDS             645..2273
                     /locus_tag="ACGE4H_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366087.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
BASE COUNT          911 a          267 c          432 g          671 t
ORIGIN      
        1 attgatttca ggaaagtatg actatgcttt tgattttgag ggtgaaaagg attacaaaag
       61 tgttgctgtc tttcaatctg attttgagaa gctaaaaaac tatgctggca ggagaaatgt
      121 taattttgca agactactag aagagttcta ctccgaagag ttgggagtag gaaatgaata
      181 caagagagaa acttatgcga aattgtctca taagttatcc caagcgctta aagaagtgac
      241 agaagttaaa gcggaactag atgctaaact tgaagaagtg aaaaaatatc aaacagagag
      301 gaagaacaac atgaagaatc aagtggaatt tgacgaacag aaagaatttg aaaaagcaag
      361 aaaacgctat aacaaaatta tgaatgaact tagctatgaa cacttaacag ttcatgaata
      421 attgagtgag ggtacagaaa aatatacgat cgcagatatg gttaatgaag tggaaactct
      481 tttagcttct ttctttgaaa atggtcacgt aaacgatgaa tggcgaaaag aagaacctaa
      541 aagggttaac gcttatattg ctcgattaaa aaggtttatt gataaatata aggagaatag
      601 tgacaaattt tctaaagata aataaggaga gagtgtgatt acatttgcca gaaatcgaag
      661 gaatattttc gccaaaaata ggtaaaaaac agttagattt cagcagtttt ccgagcgtaa
      721 atagagatta taatctaggt gatgaggtac agatactaaa tattgatttc aaagaactag
      781 acttcgctgt tgctgtttcc aacaaaccca aggcattgga acgagtgaaa aaaatatacc
      841 aagagaatga tcaaaatatc aaaaatatga tcaaaaagta tagacgagat aattcaaaag
      901 ttgaagacgt tttattcaac ggtacggcaa aacatgattt gaatgtttat atggcattag
      961 gagtggcttt gtactgtaat tcaattaatc agacagatat tttggttaat atcgtgagga
     1021 atagctatca aaaaatatta aaaataagta gcgaaacaaa cccttattat gcaatgaaat
     1081 atcttgggga taatttatta aatgataaaa aattgatgca caacactgaa gagctgttat
     1141 ctaatttggc tgcgattaac tttctattca aaacaggttc tgaagattat tttatagata
     1201 acgtactaaa aggtgcttac ggaacagcat tttatccgct ttacaatatg aaacagcata
     1261 aagccgaaca aaaggataac aaacttgaaa ttaaaaagat gaaagaacta ttaggtatag
     1321 gatttgtaaa acccaattca aaagtcacta ttgcttcgtt agcaactact aacaatgatg
     1381 ggaaaatagt aaatgggttt gacgattcag atatagatat attgtcaaaa tcatattttt
     1441 ctttgtatgg agattttgca gatttgatgg gtattgatag ttacttagtc aataattttg
     1501 agatcaaggg tagcgagctg aatgatattg tgtatttcgt tttaatctct ctatatgcaa
     1561 ttgatcagaa taccgagtac cgacaaattg actatttgct agttttttat agtttgttat
     1621 atcagttcgt tttaactcgg ttgtttaaaa atgctaagga agaattgatc ctgaattctg
     1681 attatgaaat agcaaaacaa attgcaagta ttagaaaaga aaacaaatct gaagtaatca
     1741 aattaagaaa agaattaagt attgcgagta aagaaaacgg tcggttagaa tctttagtca
     1801 aatcggcaaa aaaagaagag gaacttttga agcaagagat taaagaatta aaaggaatca
     1861 gaaaagaact tgaacagctg aggtttgaaa ataaagtatt aaagcaattg caggagcaag
     1921 aacatagaca atcattaaat ggtaaaactg aagagccttc actgtctgaa atgatagctt
     1981 atctaaaaca taaaaacatt cttttagtgg gaggacatgt aaattttcaa aataaattaa
     2041 ctgaactatt accaaatatt aaaatgtttg atgtaagcga aaataatgga aagatactta
     2101 aaaatttgaa tacgtgtgat gcgatttttc tatatacaga ttacttgaat catggtctat
     2161 attatcgtgt tatgagtgaa atttctaata aagataagat attttatttg gataatcatg
     2221 tgaatatgaa cttaacaatt aaacgtattt acgatctttt atcacttata taaagagaga
     2281 a
//
LOCUS       DAXWLB010000232         2271 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXWLB010000232 DAXWLB010000000
VERSION     DAXWLB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.238"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            192..446
                     /locus_tag="ACGE4H_002724"
     CDS             192..446
                     /locus_tag="ACGE4H_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8366088.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACGE4H_002725"
     CDS             447..764
                     /locus_tag="ACGE4H_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8366089.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="ACGE4H_002726"
     CDS             1323..2258
                     /locus_tag="ACGE4H_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366090.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DAXWLB010000233         2269 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXWLB010000233 DAXWLB010000000
VERSION     DAXWLB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.239"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4H_002727"
     CDS             complement(<1..1299)
                     /gene="ltrA"
                     /locus_tag="ACGE4H_002727"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFF8366091.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHI"
     gene            complement(1868..2245)
                     /locus_tag="ACGE4H_002728"
     CDS             complement(1868..2245)
                     /locus_tag="ACGE4H_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366092.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          548 a          511 c          413 g          797 t
ORIGIN      
        1 aatatgactt ttggagactg accaaatacc ttttcttgta tttgaccatt catacgcttt
       61 cgctttatcg atccctaaac gaatcagatt ttttcttttg gttcttggat tcttccattg
      121 tttccaaata tattgtctga ttcttcgctt caaccaaccg tttaactccc tcatgaattc
      181 tttcatctca ccaattccat agtagttgat ccaaccagtc attagttgct tgatttcttt
      241 taaaataagt tggatacttc ttccacggtt tcttttagtg agttgtttga gcttgctttt
      301 cacacgctgt tttgcttttg cgtgagggcg aattttaata cctttctttg ttggcagaat
      361 gcagaatcca agaaactttc gtttcaatgg acttcctacc gcactctttt ctcgatttac
      421 cgtcaacttt agttgttctt cgagaaattg aatggatttc ttcatcacac gctgtcctgc
      481 tcgtttgctt ctaacataga tattgcagtc atcggcgtat cgaacaaatt tatgtcctag
      541 attttctagc acttgatcaa attcgttcag gtagatgtta cttaacaggg gagagagatt
      601 gcctccttgg ggcgttcctt cttcagttgt tgtgatgagt ccattttcca ttacaccact
      661 cattaagtat tgacggatca gttttaggac ttgtttgtcc tttactttcc gctcaatgaa
      721 atacatcagc ttatcgtgat ttacggtgtc aaagtaggct ttcatatcaa tgtccaccac
      781 atatcggtaa ccttgttcat aataagattt tgctttcttc actgcgtcat gtgcgctgcg
      841 atttgggcga aacccataac tattatctga gaattctggt tcaaatagcg gttgtaacac
      901 ttgattgatt gcctgttgaa gcatacgatc aataaccgtg ggaattccta gttttcttac
      961 accgccgttt ggtttcggta tttctactcg caaaacaggc tgaggtttat agttcccttg
     1021 gcgtaaattg gatagtaatt cctcgcggtg ctctttcaag taaggaagca gttgatcgac
     1081 ggtcatccca tctgcaccag ccgccccttt atttctgact acttgaagat acgcttgatt
     1141 gagattattc cgatctagaa ttctcccaaa cagattttct acaccatctt gtttcctctg
     1201 ttcaccgcaa gaagtgctgc gcacttcatc atacccttcg tgttccacac tagctctttg
     1261 atgatagcca agttttcttg ttttctgcgg tcgtcgcatc ttctcacacc tcctaaatta
     1321 ttcaagattc ttattgttca gtccttcgta acggtcagtg tcgttactac tatgacctcg
     1381 gctgacttct catgattcac catcatgtcg ccatgatcgt tgtgctggtt tgttttcatt
     1441 caacgcccac aacaccctca tgagatctcc cccggtaaga gtgaaaactt tcctcccatg
     1501 taactgctag atttactgta taggtttcgg gcagtattgg actttgtctt gtgatgaaga
     1561 cttatccgac ctaattcagc cttgtatcta gtttctgttc gtcagttcag gagtttgcgt
     1621 ccgacttcct tcagccacaa cctcacgatt gccgccttgt ctttcgctaa cagttcctac
     1681 tgccaagcct gtagtggact ttcaccacca agttttcacc catgcagggc gcacaccaaa
     1741 aaagaagcag atccgttagc ggttctgctt cttttagttt attatatttt cttatcaata
     1801 ataggaacct gattttaaaa gcggctacct ctttaccgtg cctctttaca tacaaaaaat
     1861 agaggaatta agcgtaaatg gcttggctat tataactaaa atgctggtta tggagatgtt
     1921 gcagcaagtt ttcgagaaaa gcgtattcca tctcttgtcc attgaataag atctggatac
     1981 gataatgatt tttgaaacgc ttctgttcat gaatctgact gattgaaagt tcgcctttgt
     2041 gttctgtctg ataaagaaaa gtccttttac ggcattcagc acagcgcttg gttgctcggc
     2101 ggatttttcg gaatgtataa aaactagtat ctttattgca taaataaacg tctttgatcg
     2161 acagcaaatc attatataga caggaacgtt cttcagctgt taattctgat aaaaaaagct
     2221 gaccttgttt tattgcatct tccatcggca agtgcctcct cgaacccat
//
LOCUS       DAXWLB010000234         2238 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXWLB010000234 DAXWLB010000000
VERSION     DAXWLB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.240"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            715..1047
                     /gene="traE"
                     /locus_tag="ACGE4H_002729"
     CDS             715..1047
                     /gene="traE"
                     /locus_tag="ACGE4H_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFF8366093.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1666..2007
                     /locus_tag="ACGE4H_002730"
     CDS             1666..2007
                     /locus_tag="ACGE4H_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFF8366094.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          784 a          295 c          462 g          697 t
ORIGIN      
        1 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
       61 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
      121 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
      181 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
      241 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
      301 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
      361 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
      421 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
      481 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
      541 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
      601 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
      661 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
      721 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
      781 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
      841 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
      901 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
      961 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     1021 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     1081 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     1141 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     1201 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     1261 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     1321 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     1381 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     1441 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     1501 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     1561 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat
     1621 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     1681 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     1741 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     1801 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     1861 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     1921 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     1981 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     2041 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     2101 ttatttagtc taatggtgat aagtcaagat gaaatttaag attttcttta ttgagtaata
     2161 tgttttgaag cgcacgaaac tagacccgat caaaatcaaa tttttttcgg gttgattcaa
     2221 ggatttgcgt caataacg
//
LOCUS       DAXWLB010000235         2218 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXWLB010000235 DAXWLB010000000
VERSION     DAXWLB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.241"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(593..1696)
                     /locus_tag="ACGE4H_002731"
     CDS             complement(593..1696)
                     /locus_tag="ACGE4H_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFF8366095.1"
                     /translation="MANKNGYDIEMSYVVAPGETLKEVAEELGLSHKDLAERLGITPK
                     TISKIVNGTAPITPETALKLERVLGISSNFWNQLELNYREKLTEIEAEAKLKDEIEIL
                     KEYIPYNEIANAGFVPKTRNLKEKVENTLNYFGYSSLKIFIESVNDNQLLAGAYRIKE
                     VDKVNKLALLAWIRAGELEAQEIEAEIFSKEKLKNSLSELRNLTLQSNPSIFIPKLQK
                     ICASFGVKVVFIPEVKGSKVCGLTRWLTPKPQAIIQLSLRYKSNDNLWFTFFHELGHI
                     LKHNKIPFYTSSNEYIDSIEEQEADEFSANTLIPRDSYNDFVEQGCFTRNSICNFAEM
                     ISIHPGIVLGRLQKEKYVQWNQYNDLKTWYSWK"
     gene            complement(1686..2015)
                     /locus_tag="ACGE4H_002732"
     CDS             complement(1686..2015)
                     /locus_tag="ACGE4H_002732"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8366096.1"
                     /translation="MIVRARKRKLQKILADSKLINLEFGKQIGDKVKQRIAELLAAET
                     LEDISHLPPTKLHSLSGEYEGCFAVKLTGNFRLVFKAYTCEEEFTTQKSNTEIIVIEE
                     VVDYHGK"
BASE COUNT          687 a          422 c          308 g          801 t
ORIGIN      
        1 agtggttttt ggggaacgct ttaaactctt ctttattatc tcacaatata acccaaatgt
       61 ctttctattt taaaagtcaa agtaaaactt tttaaatata cagaagtata actatgtgaa
      121 acatcctttc attaacccat aaaaggatac ataaaaaaag ccactcattt gagtggcaat
      181 gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga acaattcaat
      241 tggctacatt aatgtaccct gtaggactcg agcctacgac cggacggtta tgagccgtct
      301 gctctaacca actgttaatc agtgttccaa agggagtgat tatactctgc tattttcaaa
      361 ctttcttaaa tcgtctatga taatctcagt ttatggtggc aaaatggtgg caagaaaaaa
      421 agtgagtatg cccgctactt gcgcaatcgg agtactgctc gatcatttat ttggaaacag
      481 gctacacttg agggtttgga agagtcaaag cggttgagcg gtgaacggaa aaagaactta
      541 actacgtata gttaggttct tttcgtttat gttttatcgg tagaataatt ttttacttcc
      601 aagaatacca agtcttcaaa tcattatatt gattccattg tacatacttt tctttttgca
      661 accttcctaa aacgatcccc ggatgaattg agatcatttc cgcaaaattg caaattgaat
      721 ttctagtgaa acaaccttgt tcaacaaaat cattatagct atctctaggt attaatgtat
      781 tagcagaaaa ttcatctgct tcctgttctt ctatactatc aatatactca tttgaactag
      841 tataaaaagg aattttatta tgctttaata tgtggcctaa ctcatggaaa aatgtaaacc
      901 ataaattatc attagatttg tatcttaaac taagttgtat tatagcttgc ggtttggggg
      961 ttagccacct tgttaaccca cacactttac tcccttttac ctctggaatg aatactacct
     1021 tcactccaaa actagcacaa atcttctgta atttaggaat aaatatacta ggattggatt
     1081 gcaaagtaag atttctcaat tcagataaag aattttttaa cttttcttta gaaaaaattt
     1141 ctgcctcaat ctcttgagct tctaattctc cagctctaat ccacgccaac aaagcaagct
     1201 tatttacttt atctacttct ttaattcgat acgctcctgc taacaattga ttatcgttaa
     1261 cactttcaat aaagatcttt aatgaagaat acccaaaata attaagcgta ttttcgactt
     1321 tttccttaag gttcctagtt ttaggaacaa atcctgcatt tgctatttca ttatatggta
     1381 tatattcttt caaaatttcg atttcatctt tcaacttggc ttctgcttca atttctgtaa
     1441 gcttctctct gtagttaagc tccaattgat tccaaaaatt tgaagatatg cctaaaactc
     1501 tttctaattt taaggctgtt tcaggtgtaa ttggcgcagt tccgttaacg attttagaaa
     1561 tagttttcgg tgtaatccct aacctctcag ctaaatcttt atgagataac ccgagttctt
     1621 cagctacttc tttcaatgtt tctcctggag caactacata ggacatttct atatcgtacc
     1681 catttttatt tgccatgata atccaccacc tcttcaatta ctattatttc tgtattactt
     1741 ttttgtgttg taaattcttc ttcacatgta taagccttaa aaactagcct aaaattaccc
     1801 gtcaatttta cagcaaaaca cccctcatat tccccagaga gagaatggag tttcgtgggc
     1861 ggaagatgcg aaatatcttc taatgtttct gccgctagca attcagcaat tctttgctta
     1921 actttatctc caatttgttt tccaaattct aaattaatta gcttggaatc agctaatatt
     1981 ttctgtaatt ttcttttcct cgctctaact atcatccgac ttaacctttt aggttaacaa
     2041 ctctaaatat agtataccac aataatttgt ttttcaagca ctttatgatt tacttaaaga
     2101 ttaacctaaa ttctttcgct tatagtagtt tctctttata gattatcaaa agttgtaaag
     2161 attttaaagt caattttgta taaaaaaaac ttttttcgtt ctatgtactg cccctcaa
//
LOCUS       DAXWLB010000236         2213 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXWLB010000236 DAXWLB010000000
VERSION     DAXWLB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.242"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            46..609
                     /locus_tag="ACGE4H_002733"
     CDS             46..609
                     /locus_tag="ACGE4H_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366097.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(670..1035)
                     /locus_tag="ACGE4H_002734"
     CDS             complement(670..1035)
                     /locus_tag="ACGE4H_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFF8366098.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1111..1356)
                     /locus_tag="ACGE4H_002735"
     CDS             complement(1111..1356)
                     /locus_tag="ACGE4H_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8366099.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1426..1680)
                     /locus_tag="ACGE4H_002736"
     CDS             complement(1426..1680)
                     /locus_tag="ACGE4H_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366100.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1844..2167)
                     /locus_tag="ACGE4H_002737"
     CDS             complement(1844..2167)
                     /locus_tag="ACGE4H_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366101.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          827 a          368 c          384 g          634 t
ORIGIN      
        1 ccgttattgt agagtgattt agcattctta tttttaaaaa taactgtgtt atatattaaa
       61 aaaggcataa tagttataag cttttttaaa gaatatttag aaattattaa ttttcgtctg
      121 aaaacaaaaa aagaaagcgt tattatttta gtctattttc agggggggaa ccttatgata
      181 aaaaaactat ttttactatc aatattaacg ctagcaatgg caggttgttc tgactctggc
      241 ttagcaactg aactgatcac aaaatcaact gaagccacga cagaggagtt aaaatgtgga
      301 aacgatcaca gtgactacag aggcttaaat cgacgatgca tgggaatatt aggcagaagc
      361 aattgtaagc atcgatgtag taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa
      421 agaaagatca aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga agatgcggta
      481 cttttaaacc acgtcaggag atgttcattt atgtctaggg aagttggcga tgaaccaaaa
      541 ctagagtacg cttttagttt tgcacgtgag gatgaagata accctaaaac ttacgacatt
      601 ttagcttgat ctaagatggt taaagctttt taatgaagta agaggagata gaaggctttt
      661 ctagttatat taggtaagat aaattccttt ttgctccaaa tttgctaaac attctgctag
      721 ataaacttca tcgtgttccg gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa
      781 aaatgcttcg catgatttgc cacgatacat aggatttttc cactctggtg ctgggctgta
      841 accacgtttt tccatttcat ccatgacaag ctgatgatat tggaataatc tataaggaga
      901 atagtgaaaa acataatcca cagtagcatg tttctttccc cagccatttc ctcttaatgc
      961 acagcattcc cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa tcaatttttc
     1021 atgccataat cgcatagaaa tgtctccttt atatatagat tgttaacctt agacgttaaa
     1081 tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg
     1141 accaattagg taatcaacgg aaatatcgta aaagtcagcg atatagatta attcaatgat
     1201 gcttggaatt tcttctcctt cttcgtactt acgatatttg aataaaggaa tacttaacgc
     1261 gctagaaaca gctacttgag ataaagatcg gccagttctt aagaaattca gacgacaacg
     1321 taaagtatcc agtaaaatag cttcttcgat actcatttaa tcacctttca ttatagtaga
     1381 atagttataa tgactataga aagctacaca tgcaatcaat agtactcaat gttttttatt
     1441 taaacgtatc aaataaagaa tgacgatcaa taaagacaaa accagtcctg ataattcttc
     1501 tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa actttccaca taaagtagat
     1561 agccggaaga gtcaaaatag ctaaataaaa ccgttctttg ttccaattca taagataaac
     1621 aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa actctcacca acgtaatcat
     1681 gtaacggact cctcaacgat tcatataata aaagataaca tgttattgca tacatcacaa
     1741 atgaaatgct aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt
     1801 tttctgctga taagcaggaa ctgttccgct gaatgtgtct tatctatcga gaaaaaatca
     1861 atggattgat atctaagtaa ttcaatacat ataaaaagag gacgatcgca ttcactaaaa
     1921 aggaaagaat gatgatccca tagcgaatgg aaacatattt aacaaatact tttgtttgaa
     1981 tacgggcata tctttttcct ttatagctga gcatcatgac aagaagcaaa agggtaatca
     2041 caccttgaaa aatcattcca ataaacagaa ttcctgaggt ttgataattt aattggagaa
     2101 aataagctgc tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa ataatcactc
     2161 ctaacattga gaataaataa aagaataaca ttttttctaa aataagcaaa taa
//
LOCUS       DAXWLB010000237         2183 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXWLB010000237 DAXWLB010000000
VERSION     DAXWLB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.243"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            19..1632
                     /locus_tag="ACGE4H_002738"
     CDS             19..1632
                     /locus_tag="ACGE4H_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFF8366102.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          682 a          387 c          437 g          677 t
ORIGIN      
        1 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
       61 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
      121 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
      181 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
      241 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
      301 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
      361 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
      421 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc
      481 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
      541 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
      601 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
      661 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
      721 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
      781 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
      841 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
      901 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
      961 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
     1021 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
     1081 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
     1141 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
     1201 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
     1261 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
     1321 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
     1381 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
     1441 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
     1501 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
     1561 ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
     1621 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
     1681 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
     1741 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
     1801 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
     1861 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
     1921 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
     1981 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
     2041 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
     2101 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
     2161 tttttttgtt cctatttatt taa
//
LOCUS       DAXWLB010000238         2135 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXWLB010000238 DAXWLB010000000
VERSION     DAXWLB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.245"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..788)
                     /locus_tag="ACGE4H_002739"
     CDS             complement(<1..788)
                     /locus_tag="ACGE4H_002739"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFF8366103.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYEKKVISL"
     gene            complement(760..1656)
                     /locus_tag="ACGE4H_002740"
     CDS             complement(760..1656)
                     /locus_tag="ACGE4H_002740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8366104.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1646..>2135)
                     /locus_tag="ACGE4H_002741"
     CDS             complement(1646..>2135)
                     /locus_tag="ACGE4H_002741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366105.1"
                     /translation="AINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVD
                     CGVIYDNAEKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIV
                     KRYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMND
                     EK"
BASE COUNT          736 a          362 c          255 g          782 t
ORIGIN      
        1 cttaagctga taactttttt ttcgtaaata ttcatttttg ataatttatg aactaaaaaa
       61 agatttgctg aaaaaaagag agcaaacatc actacgtgag aaatcgatgt actgtttcta
      121 tcaaaatatg catcatactt cgtatttata aatattgaac tgatataact ttgtccaata
      181 gagaatagga gcaagctaag gattgtaaca tatccagtaa tctttttatt aatttttaca
      241 ataaaaaaag ggagataaaa atatgcaaga ttatggaaca gtccagccac taagataaaa
      301 aaaatatact taaatatagc tttgctgcct cgcaatttag ttagatgata aaaagctagt
      361 aatatgaata tcaatgcgct aaacatacgt acttgtattg cttgtgaagt aaaaccaaaa
      421 ataaaaaaca agaacaaaac taaaaaaaca ttagttgtga ataatctgat gacatacaat
      481 aaagctgaga gtaagacagt agaaataatc aataaaaaaa tctgatactg actggaaata
      541 aaggtacata cgtacataac agctctgaat cccggttcaa tactatctga actaaagtcc
      601 ataccaaatt gtgctatccc attatacaag acagaatatg cataataatc agcgttttgt
      661 gtgttaaatg ccatgatgat ccacattatc aaaagtagca ccccagacaa aatcttgttc
      721 gatgtactcc attttttttg tgaaacactc ttttcttgat taatctccaa tgacgcctct
      781 gtatccatct cgaacccccc ttatagccgc tctaaatcta ctgagcttat cagtctcaaa
      841 agctattgca ataaaaaatt tcaataatct tttattcata aggcgggaaa attccttatc
      901 acttttatac attcttttca ccaatgttga atttcttgca ataaaatact ttcgtgttga
      961 attatgattg tctattttaa ttttttttga tcctattttt aaattttctc catctccaac
     1021 gtgatgataa aaatagactt gatttgttac ataaatgtta tagccattat ttgcaaccct
     1081 tttacaaaat tcaacatcaa tctgatcaat aaaaaattca tccttcatat ttccaacatc
     1141 gtcaattaat tcacttttga aaaacattcc agaggaattt gcaaatgcta ttttcatgga
     1201 accactagtt gaagcaaagt gtttttttac gtatctccct ctctcatttt tgcaggtaaa
     1261 agagacagat tcatcattgc tattctcaaa gtaattggga acaataatcc caatgtcctt
     1321 atctttcgct tcaatattgg taaactcttc taataatttt aaagaaagat tcttttgtat
     1381 gatggtatct tgatctagta aaaggatata atcacatttc tcttttttgg caatttcaat
     1441 acctatgttt tgagctgtcg caagcccaat gttctcattc atttttttta aaattaagtt
     1501 ttccatcatt tcaaattgaa aagagttaat attatctgag ccgttatcca cgataataag
     1561 tttgttctgt ttctcttgta ttaacgtatc cactaataac tttatgttat tatctggatt
     1621 gtaggtaaca ataattatcc caattttatt tttcatcgtt catcaccttg tacacttcat
     1681 tccatttcat tttcaatact tgctcagaga atatagtggc gtaaattttt ctaacatttt
     1741 catttatgtt tttattacac tgataaaatt tcaacatctc attttcatca acggtactat
     1801 tataattgat tccgacatta tatcttttaa ctatttcttt cgtatctcct gataaatttg
     1861 aaattatagg taatccaaac ctaaaatact caatcgattt cagacttaaa ccaattatgg
     1921 tatttgactt aaataaattt agtccaaaat gacatttatc gagtataact tttttttctg
     1981 cattgtcata tataacaccg cagtcaacaa attcaatatg atttttttca caaatatcag
     2041 ctaatttagt tttgctttca ccttttccaa taaagtaaag atttattttg tttccaaatt
     2101 ttcttttgca tgttttcaat aattcattaa tcgct
//
LOCUS       DAXWLB010000239         2135 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXWLB010000239 DAXWLB010000000
VERSION     DAXWLB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.246"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            18..536
                     /locus_tag="ACGE4H_002742"
     CDS             18..536
                     /locus_tag="ACGE4H_002742"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFF8366106.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            664..1347
                     /locus_tag="ACGE4H_002743"
     CDS             664..1347
                     /locus_tag="ACGE4H_002743"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFF8366107.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1656..1729
                     /locus_tag="ACGE4H_002744"
     tRNA            1656..1729
                     /locus_tag="ACGE4H_002744"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1690..1692,aa:Arg,seq:tct)
     gene            1888..>2135
                     /locus_tag="ACGE4H_002745"
     CDS             1888..>2135
                     /locus_tag="ACGE4H_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFF8366108.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSML"
BASE COUNT          722 a          334 c          434 g          645 t
ORIGIN      
        1 gaaagaagtt gaaaacgata cctgattaca aaaaaataac ttttataagt tctataacgt
       61 tgattatatt tttaattctt attcttctcg tcgtcttggt tccccgaatt tttggtttga
      121 ctccttatat tattgaggac aaagaaatga gtccatatta tgcacaagga aatctgatat
      181 atgtacgacc gaaaaataca aatgagattt tagtcggaga tattattaca tattatgaaa
      241 attcaggaaa aaaaatctcg acgcgtcgcg taatagccgt tgaaaacaac cacgaagatt
      301 tttatacaaa agctgataca aagacttata ttgagcctgg agtagtaaaa aagagaaata
      361 ttatcggtag tcccatcttt cagattccat atattggact agtcctttca aaaacggcat
      421 ttgcatggat tcaggggtta ttctttacga ttgctttttg cctaacagga atcacagcat
      481 ggaatacctt tgtagagttg aaaaagaagc ggagacgaac acagaaattt ttgtaagaat
      541 gttaaaaaca taaatgtatt ccagagatcc aacgactctt ctgaacatga aacacgagaa
      601 aacactcatg cagcttgtag atttagtcag ttgttcgtat aatgtgtgaa gaggaggagt
      661 agtatgcgca atgaaatgat gcatcgtcct gtagtcaaag aagaactatt ggattttatg
      721 cgtacccagc aaaaaaaatt accgggtgag ctaggcaaac tggaagaaga agctcatgta
      781 gcagaagtac cagttattcc ccatgaaaca gttgtatttt tgaaattttt acttggacag
      841 cttcagcctg aacgaatctt ggagatcgga gcagctatcg gtttttcatc aagtgtcatg
      901 gccacgacaa tcggagaaaa cgctcatgtt acgacaattg atcgtttcga tgtcatgatc
      961 gagaaagcta aaaaaaacta tgaacgtcta ggactgacag ataaagtgac tttgcttgaa
     1021 ggtcaagcag cagatatctt gccggagttg tctggtccgt atgactttat ttttatggat
     1081 agtgcaaaat caaaatatat tgaattttta cctgaatgct tacgtttgtt aagaaaaggt
     1141 ggcgttttga tggtagatga catcttccaa ggaggaacga tcttgctccc agatgaagag
     1201 attccccgtg gcaaacgagc tatccatcgt aaattaaatg aatttcttcg tgttgtgatg
     1261 gatcatcccg atttgacatc gacaatcttg ccattaggtg atggtgtgat cttaatgaca
     1321 aaggaatcag aaacaatcga gttgtgatac atgattatgg cagcctgtag ataatcgatt
     1381 gaatcgaggg ctcaattcga acttgatgta aaagtttcta tatttgtaag tcaattaaag
     1441 gatacaggaa atctaaataa acaggagtag catgaagtca atgtagctcc tgttttcaat
     1501 tgataacctt agaacagctg aaggagaatg tctcgtttta aaagattgat ttagtagttg
     1561 ggccaaaata attcattatt tgctaattaa ataactattt cgtattgaca gaagcaattt
     1621 aattatctat aattattgat gttccgctga ttcttgtcat agtagctcag ctggatagag
     1681 catccgcctt ctaagcggac ggtcgggggt tcgaatccct cctgtgacgc taaaaaaccg
     1741 ttagaaactg aggtttctaa cggtttttct ttgttttttg tgccaatatt gtttgttttc
     1801 aaaggcaaat aaaataaaaa aacatcctta tcgagtacaa ttataaggag caaaaaaata
     1861 aaactcatca gaaaagagga agaagatatg acgatactat ctagaatttt agtgactcta
     1921 gttgcaattg aattttttta tatcatgtat atcgaaacgg tgagaactga ttctgacacg
     1981 acaagtaggg tttttaaaat gagtaaagaa gagttaagca gaaaatctgt tcaaacattg
     2041 tttaagaacc aaggagtgta caatggacta ttaggagtgg gactacttta tggagcttat
     2101 ctttcttccg catctaaaga aatcacaagt atgct
//
LOCUS       DAXWLB010000240         2110 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXWLB010000240 DAXWLB010000000
VERSION     DAXWLB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.247"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            121..2109
                     /gene="treP"
                     /locus_tag="ACGE4H_002746"
     CDS             121..2109
                     /gene="treP"
                     /locus_tag="ACGE4H_002746"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFF8366109.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
BASE COUNT          613 a          409 c          461 g          627 t
ORIGIN      
        1 aaaacgatat cattttctct cgtacttttt aataaaacgc ttgcaactct ctccgaaaaa
       61 aagtatagta aatgaagaaa caacttgcat agacaagtta agagaaaggg gtttattgat
      121 atggcaaaat atcaagcaga tgccgagaag ctgttacatg atatcggcgg aaaagaaaat
      181 attgctgcag ttagtcattg tgcgactcgt atgcgttttg tcctgaatga tccaggaaaa
      241 gcggacgaaa aagcgatcga agatatacct agcgtaaaag ggatgttcac gaatgcagga
      301 cagttccaag tcatcatagg aaacgatgtt tctacttttt ataacgactt cgttgcagta
      361 tctggagttg aaggggtttc caaagaacaa ggaaaagcag ctgcaaaaca aaaccttcat
      421 cccgttcaac gtgctattgc cgttttagca gaaatcttta caccactgat tcctgcaatc
      481 attgtcggtg gattgatcct tggattcaga aatgtacttg aaggtatcca gtttgaaagt
      541 cttggtggaa caatcgttga acactcgaag ttttggaatg gtgtcaatgc ctttttgtgg
      601 ttaccgggtg aagcaatttt ccattttctt ccagtaggta tcacttggag tattgcaaaa
      661 aagatgggta ctacacaaat attaggaatc gttttaggaa ttacgttagt ttctccgcaa
      721 ttattaaatg cttatagtgt agcttccaca gccgcagcag atattccatt ttgggatttt
      781 ggttttgctc aagtccagat gattggttat caagcgcaag ttattccagc catgctggca
      841 gggtttatgt tagtgtatct agaaatcttc ttccgcaaat atatccctca gtcgatctcg
      901 atgatttttg ttccattgtt ttcattgtta ccaactgttt tagctgcaca cgttatttta
      961 ggtcctatcg gctggacagt cggaagctgg atctcaaata tcgtaaatac tggattgacg
     1021 tcttctatca gctggttgtt tagtgccgta ttcggattcc tgtatgctcc tttagtgatt
     1081 actggtctcc accatatgac caatgccatc gatatgcagc tgattgcaga tttcggatca
     1141 acgaatttat ggccgatgat tgctttatca aatatcgccc aaggctctgc agtcttagcc
     1201 atcatcttct tacatcgtgg gaataaaaaa gaagaacaaa tctcgattcc agcaatgatt
     1261 tcttgttact taggcgtaac tgaacctgct atgttcggaa tcaatttgaa atacgtctat
     1321 cctttcgtcg ctgcaatggt cggctctgga ttagctggca tgtttgctaa cttgatgggt
     1381 gttcgagcta acgcgatcgg agtaggcggt ttgccaggaa tactagctat ccaagcagaa
     1441 acatgggtac ctttcatcat cgcaatgatc attgctgtca tcattccatt tggcttgacg
     1501 atcatcttta gacgtcaggg aattttgaat aagattgatc ctgctgttcc tgaaaatgct
     1561 gctgatgtac aacttcaaac agcaaatggc gcaacagcaa cgccacagtc tttcgaacct
     1621 gtttcagcaa ctggtacggc agttgcaaca aaagaaacat tattcgcagt cgctgcaggg
     1681 accatcaagg aaataactga agtaaatgac ccagttttct cacaaaaaat gatgggtgac
     1741 ggttatgctg tcgaaccgtc aaacggcaaa gtctatgcac cagtaaacgg taaagtgacg
     1801 agtgtctttg aaacaaaaca tgccattggt attctttcta atgaggggct ggaagtactt
     1861 gttcacatgg gattagatac tgttgaacta aagggtgttc ctttcaatgt attcgtgaaa
     1921 gaaggatacc ttgtcacacc agagaccctg attgctgaaa tggatttgcc agaaatagaa
     1981 caagctggca agaaaactga cattattgtt gctttgacca ataatgaaaa agttgcagga
     2041 ctttctcttg accaatcagg acttgttcgc ccgggcgaag cagtcggtaa agctgaagta
     2101 aaatcttagt
//
LOCUS       DAXWLB010000241         2086 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXWLB010000241 DAXWLB010000000
VERSION     DAXWLB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.248"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            84..407
                     /locus_tag="ACGE4H_002747"
     CDS             84..407
                     /locus_tag="ACGE4H_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFF8366110.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            589..1959
                     /locus_tag="ACGE4H_002748"
     CDS             589..1959
                     /locus_tag="ACGE4H_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFF8366111.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT          587 a          385 c          427 g          687 t
ORIGIN      
        1 cctcgttgaa gtaagtattt ctagtttatc ctaggattaa ttataaaaat aaaacattcg
       61 ttgattcgaa ggaggttaaa cagatgtttg aaagttttga tagcagccgc aaccgttacg
      121 cttcagttgg tgtcgtatca agtttgccag atgaattgat tgacagtata tggtttatta
      181 ttgacctaga tcttaaggga gtcattccat tagaaaatat attaaccttt gatttgatcg
      241 aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg aagcaatgtg gaaatggata
      301 ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca agtatacgcc tatgatgacg
      361 gtacgagaga aacgatcctt ttaccaagtg agattagaca atattaaggt gatagataaa
      421 cgtggatgtc ccttatttta cagaccaggc caagttgttg attctttgac tcaattcttg
      481 gcttcttttt atggagtttc ataaattatc attgatttct aattggtatt ctcaggataa
      541 acgctatata ctactaacta aattttaaag atataaggag tttaaaagat gcgcgattta
      601 acaaaaggaa gccccgctaa actgattttg atgttcactg ttcctttact gatcggaaat
      661 gtttttcagc agttctacaa tatgatcgat atgatcatcg tgggtcagac gctaggtaaa
      721 aatgcgctgg cagcggttgg tgcaactgga agcctgacat ttttgatcat tggctttgcc
      781 caaggtttaa cagctggttt agccattatt accgcacaac gttacggtgc aaaagattat
      841 cggggtctga agaaaagttt tgctgctagt gttgtcatta gtcttgtcgt cactgtgatt
      901 ttaactgtcc taagtctcct gtttattcgt ccgatgcttc aattgatgca aactcctcct
      961 gaaatacttg atcaagcaca aacatttatt tcaattattt tattagggat ctttgcatca
     1021 gtcagtttca atttgctttc taatgtcatt cgcgcacttg gtgacagccg cacaccacta
     1081 ttctttctga tcattgcggt catcatcaac gtcgtacttg atttgatctt tattatctat
     1141 tttggcatgg gtgtggaagg tgcagctatt gctacagtaa tcgcacaagt ttcttccagc
     1201 gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt tacagttacg caaaaaagat
     1261 ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg ctgctttgcc aatggcgttt
     1321 caatcttcga tcatcgctgt tggtgctatc gtgttacaag cagcactaaa tagtctaggg
     1381 acagatgtcg tagctgctca agccgctgct ggtcgtatcg accaatttgc aaatcagccg
     1441 atgatgtcat ttggtattgc aatggcaact ttcactgcac aaaactatgg tgcgaaagaa
     1501 tatggccgaa tcttgcaagg ggtacgtcaa actttactga tgagtgtcgg atttagtctc
     1561 ttagcaggtg ctatcgtcat tttcttcggt catttctttg ttcaattgtt tgttagccca
     1621 tcagaaacac gtgtatttga attagcacaa acctacttca atatcaatgg gtctctttac
     1681 tggatcttag cagtcctgtt cattttacgc tacacacttc aagggcttgg acaaagcaag
     1741 atccctacga tcgccggtat gatggaattg cttatgcgtt cttttgcagc tatcgttctt
     1801 acaggaatgt ggggataccc aggagcagca gcagcttctc ctttagcttg gatcggttct
     1861 gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac aattgaaaca catgggtgaa
     1921 gaggaacatt tcacattaga acatgcagaa gcaagatagc cttgattaaa agaaatcgtt
     1981 gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc gtttaccgaa aacaggtttt
     2041 ttagataaca aaaaggctgt gacaaaaaat gtcacagcct tttttc
//
LOCUS       DAXWLB010000242         2051 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXWLB010000242 DAXWLB010000000
VERSION     DAXWLB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.249"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>2051
                     /gene="acm"
                     /locus_tag="ACGE4H_002749"
     CDS             <1..>2051
                     /gene="acm"
                     /locus_tag="ACGE4H_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFF8366112.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     L"
BASE COUNT          804 a          338 c          404 g          505 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg a
//
LOCUS       DAXWLB010000243         2047 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXWLB010000243 DAXWLB010000000
VERSION     DAXWLB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.250"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1995
                     /locus_tag="ACGE4H_002750"
     CDS             <1..1995
                     /locus_tag="ACGE4H_002750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFF8366113.1"
                     /translation="FSLGVEGTIQYFILWINPIATTLLFFGLSLYIKKPKPALAILLI
                     IDILNTLLLYLNIIFYREFTDFITVKSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIA
                     VFIGLLVWLKVKKIPIKSNPVGKPMAIAVTCLSGLIFSGNLALSEANRPQLLQRTFDR
                     SYIVKYLGIDAYTIYDGIKTGMTSSVRAHASSNGIDEVLDYTKKHYAEPNPETFGIAK
                     GKNVIVLHLESFQQFLINMKVDGQEVTPFLNSIFQNQATISFDNFFHEVGQGKTSDAE
                     NMLETGTFGLPQGSLFTELGSDNVFQAAPAILGQKQGYTSAVFHGNVASFWNRDHVYK
                     NLGYDNFFDRSYFDESDETLGYGILDKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYY
                     TDDKNFDFPSLNTGNSTVDNYVRTAHYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFG
                     ISNTDNKDLASALGKDPDTWDEFDNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPT
                     LLHLLGIDDKDYLHFGTDLLSSQHDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDT
                     KAAGIDEEISKDQEKVKTALSLSDKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNE
                     AIEKQKGEHSTSVFSKNGNKTTTNLYPVTRTDVEQQKNNQ"
BASE COUNT          672 a          365 c          407 g          603 t
ORIGIN      
        1 ttttcattag gtgtggaagg tacgatccag tactttatct tgtggatcaa cccaattgcg
       61 actaccttat tgttttttgg cctttctttg tatattaaaa agccaaagcc agcgttagct
      121 attcttttga tcattgatat tttaaatacg cttttgttgt acttgaatat tattttttat
      181 cgggaattca cagatttcat tacagtcaaa tctgtattag gcttttcgaa agtgtcgcaa
      241 ggattgtcag gaagctcctt ttctttgatg aagccccatg atgtcatcta ctggctggat
      301 atcgctgtgt tcattgggct actggtttgg ttgaaggtca agaaaatacc tatcaaaagc
      361 aaccctgtcg gcaaaccgat ggctatcgct gttacctgtc tttcagggct tattttttca
      421 ggaaatctag cgctgagcga agccaaccgt ccacaattgc tgcagcgaac atttgaccgt
      481 tcatatattg tcaaatatct aggaatcgat gcgtatacta tttatgacgg aatcaaaaca
      541 ggaatgacta gcagtgttcg cgcgcatgca agcagtaatg ggattgatga agttttagat
      601 tatacaaaga aacattatgc agagccaaat cccgaaacat ttgggatcgc taaagggaaa
      661 aatgtgatcg ttctccattt agaaagtttc cagcagtttt tgattaatat gaaggtagac
      721 ggacaagaag tcacaccgtt tttaaatagt attttccaga atcaagcaac gatcagcttt
      781 gacaatttct ttcatgaagt aggacaggga aagacaagtg atgctgaaaa tatgttggaa
      841 actggcacat ttggtttacc tcaaggctca ttattcacag aacttgggtc agacaatgtc
      901 tttcaagccg caccagcgat ccttggacaa aagcaaggct atacaagcgc tgttttccat
      961 gggaatgtag ctagtttttg gaatagagat catgtatata aaaacttagg ttatgacaat
     1021 ttctttgatc gatcttattt tgatgaatcc gacgaaacgt taggttatgg gatattagat
     1081 aaagatttat tcagagaatc tgcccaatat cttgagcatc tacaacagcc gttttacacg
     1141 aaatttcttt ccgtaaccaa ccacacgcct tattatacgg atgacaagaa tttcgacttt
     1201 ccatctttga atactggcaa tagcacagta gataattatg ttcgaacggc acattatctg
     1261 gatcaatcat tggaacaatt ttttacgtat ttgaaaaaat ctggtattta ccaaaattca
     1321 atctttgtga tttacgggga tcattttggg atctcgaata cagataataa agatttagca
     1381 agcgctcttg gtaaagatcc agatacatgg gatgaatttg acaacgcaca gatgcaacga
     1441 gtgccattga tgatccatat gcctggttat actaaaggga cagtcaatca tgaatacggt
     1501 ggagaaatcg atgttttgcc gacattacta catctattgg gaatcgatga taaagattat
     1561 cttcattttg gtacagactt attatcttct cagcatgatc aagtagttgc ttttcgaaat
     1621 gggaactttg tgacacccaa atacaccgtc ctaggaggaa aagcttacaa taatcagaca
     1681 ggagaaatca tcgatacgaa agcagcagga atcgatgagg aaatctccaa agatcaggaa
     1741 aaagtcaaga ctgcgttaag tctttcagat aagctgaatc aggaaaatct cttgcggttt
     1801 tatgtgccaa acggatttga gacgattgat ccgaaaaaat atgactataa aaatgaagcg
     1861 gaacgtaatg aagcaatcga aaagcaaaaa ggcgaacatt cgacaagtgt cttttcaaaa
     1921 aatggtaata aaacaacaac caacttatat ccagtaacaa gaacagacgt tgaacagcaa
     1981 aaaaataatc aataaacaat caaaccaacc ttcagtagtt agacaacatt ctaatgcttg
     2041 aaggttg
//
LOCUS       DAXWLB010000244         2042 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXWLB010000244 DAXWLB010000000
VERSION     DAXWLB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.251"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(1..1392)
                     /locus_tag="ACGE4H_002751"
     CDS             complement(1..1392)
                     /locus_tag="ACGE4H_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFF8366114.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            1518..1928
                     /gene="tagD"
                     /locus_tag="ACGE4H_002752"
     CDS             1518..1928
                     /gene="tagD"
                     /locus_tag="ACGE4H_002752"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFF8366115.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
BASE COUNT          783 a          327 c          285 g          647 t
ORIGIN      
        1 tcaattagtt actccttctt ctttagatat agttttttgg atagaaccca gatatcccca
       61 aaaaataaat gaacctatcg tactaaccaa aatgagttct gtgataaata atgccgaaac
      121 tgctattgta agaatcatta aaatagcaaa aaagtgctta tcttttacta gatttggttg
      181 tgtgaaacaa taacgcaagg ctttaaagca ttggataaat aagaaggtta acaatgtcaa
      241 tagacctaat cctcctgtag cagccatcac taagaaaata aaattatgag cagttaaagt
      301 atcttttccc attaatgtat atggtagttt atcatgtgca tattcaatat agtagcctgg
      361 agaacctccg gatatcggct tactcttgaa tatttcaatt gcacttttcc aaagttcaaa
      421 ccttaaatta gaaacatccc cgctatcagt gtcagggcgt tttaaatcaa ctttattgct
      481 gctagttgtt cctgatgaat cagatgtatc tttttttact tctgtaatat gcggaacatg
      541 tgcccatgca tgagcagtta tatctgttac ataatacatc gcaattatac ttagcaatcc
      601 tactactaat gcagttagcc atctttttaa agtattagtt ttaagaaaac gactactata
      661 acttgtaaag aaactaaaaa caaaagttgt tattaataaa caaactaatg tggtgcgtga
      721 acccgataaa gcaataaacg agaattgaat aaaaatatta attacaaaaa atattttaaa
      781 tagaagattt aaatgcttgt aagtgaccaa ataatatata gcaataatga tgacgattaa
      841 tgatataacc gcaccaaaat ttggatctgt atatacacca aataaacggt tgcctaacca
      901 ccctaaacga actgggtaat acttatattt tattggaatt tttatgccat attgaatgac
      961 aaaccagtat aatgatacta aattagctaa aaaccatacc aatgaaagta tccaggtaaa
     1021 tactttgaaa aaaaagttac tactatagtc tttgccaaac tgataaatca taaagaaaaa
     1081 gattaattgc cacatgatta acttaatatt ttcgctatat ccatatttta aattcattaa
     1141 actgcttact agcatcgcgc caataaaagc aattaatagc caatcatatt tttttagatt
     1201 tttttctcta aatgcaataa ttgcatctat aatgatcaag acaactcccg ctattccagc
     1261 aggccaaaaa agtaaagtat catatctagt aggtaatagt aaatacgggc ctattgctcg
     1321 taacattaaa ataaaaccaa aataaataaa ataccaacct ttcacatatt ttaaaaatct
     1381 ttcattcaac atttttcctc catccaccta tttctcataa atatttctta tcctttttag
     1441 gataaactgg tcctttcaat tctaacaaaa aaatgctaaa ctaacaatgt atattaatga
     1501 atttcgagag gtgaaagatg aaaagagtaa ttacttacgg tacctttgat ttgcttcatt
     1561 acgggcatat caatttactg aaacgagcaa aacaatacgg agactatctt attgttgccc
     1621 tttctacaga tgaattcaat tggaatgcga aacaaaaaaa atgttatttt agttatgaaa
     1681 aaagaaaaca gttacttgaa gctattcgct acgttgattt agttattcct gaagaaaatt
     1741 gggaacaaaa agtaaaagat ataaaagaat accatgcaga ttatttcgtg atgggcgatg
     1801 attgggctgg acattttgat gaacttgaac aactaacaga tgcacaagtg atttatttac
     1861 caagaacacc tgagatctct acaactcaaa taaagaacga acttaattct ttaaatagtg
     1921 ataaataata ttttttaaac aataaaagaa caatatatct ggataagacg taaaaaaatg
     1981 gctctataat ttctaggact gatagaattt tctgtcggtc ctagattttt tgtaaaaaaa
     2041 aa
//
LOCUS       DAXWLB010000245         2041 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXWLB010000245 DAXWLB010000000
VERSION     DAXWLB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.252"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1166
                     /locus_tag="ACGE4H_002753"
     CDS             <1..1166
                     /locus_tag="ACGE4H_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFF8366116.1"
                     /translation="KISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISPI
                     EDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIEK
                     ALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIVG
                     ECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYAE
                     FRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAIV
                     DANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVVY
                     EPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT          700 a          292 c          385 g          664 t
ORIGIN      
        1 tgaaaatttc agttttcgga ttaggctatg ttggtctagc aaattcacta ttattagcac
       61 aaaaagagca ggtcaaagct tatgatatcg tggaagaaaa aatcacgatg ttacaaaatg
      121 gcatctcacc aatagaagat aaagaagttc atgaattttt aaaacgggat gacttaaatg
      181 tcgaatttac gtcaaatttt actgatgcag taaacttcgg tgattacttg attattgcaa
      241 ctccaacaga ttatgacgaa aagaaaaact attttaatac atcaacagta gaagatgtaa
      301 ttgagaaggc tttagcaatt cgtccagatt caacattcat tattaagtcg acagttccag
      361 ttggctatgc aaatgaaatt aaagcgaaat ttggtacaga aaacatcatt ttttcacctg
      421 aatttttgcg tgaaggaaaa gctttgtacg acaacttata tccatcaaga attatagttg
      481 gagaatgttc tgctcgtggt cgcgagattg caactatgtt tcaaagaaac gcatttaaca
      541 aagatgcatc agttcttttg actcactcaa ctgaagcaga agctattaaa ttattctcga
      601 atacatattt ggcattgcga gtggcgtatt tcaatgaatt agatacgtat gcagaatttc
      661 gtgggcttga tagtaagcaa attattgaag gtgtcggttt ggatccacgg atcggttcgc
      721 attataataa tccttctttt ggctatggtg gatactgttt acccaaagac acaaaacaat
      781 taagagccaa ctatgaaaaa gtaccaagca atatcatcag tgcaattgtt gatgcaaata
      841 caacaaggaa agattttatt gctggacaga ttttggcgaa aaaaccaaat gttgttggaa
      901 tctatcgttt aacgatgaag gccggttcag ataattttcg atattcgtcg atccaaggaa
      961 ttatgaaacg gattaaagcc aaaggaattg aagtggttgt ttacgagcct acattggatg
     1021 aagatgaatt ttatcattca caagtattga aagatattaa tgaatttaaa aatatttctg
     1081 atgtgatcgt atctaatcgg tttgaagata ttttaaagga tgttgaagaa aaagtttata
     1141 ccagagatat ctttggtgat aactaaataa taatgaacgg gtacatggag gaaaatattt
     1201 tagatggtga ctcgaaataa atgtttgttc tcattgcatc tgtatctata ccgataagtt
     1261 taaaaattcc gattaggaaa atgaagttgg ctatccttgc gaggaattta ttgctggatt
     1321 attttatgaa ctaaagcaag acatcatcag aaaaaaatcc aaaatttgac aatgggattg
     1381 atcgtttacc agctgtaagc gaagtatctg acacgaaaat ggaaaatgca attactgtta
     1441 ttaccaatag ctttttttca tcgatcaagt atttgaaaac attatttgtt tgccaagctg
     1501 atcaatgttc tttcagactt ggaagaacat tgatcagctc acaagtaatg attgaagttt
     1561 aggttatggg ataaaggata agtgaaataa aagtcgtgtc tgcatctttg ttagtaccag
     1621 taaatttttc tactacggcg ctagtcacga cagtccaggc atctgaaatt gaagatgcaa
     1681 atagagtttc tgctagttta ggtattgatg ttactgtatt agcattaatc ttagagccag
     1741 tagcatccaa taggtgtagc tatagttcta atagtataaa acaatgcaga ggatatgatt
     1801 gctttatgtc tcggtgtgtc aggtcaaaat agagttccat atggggcgat ttctcaatga
     1861 ataatcttat agatataatc ttgtttgcta tttcgttatc acttttactt ttcttcgatt
     1921 atttcaaaaa taaaaatcca aaatatttta agttcttact atttatcatt actgtactag
     1981 ctgttttatc gatgacagat tttatttaac ttatctcaaa aagtagggaa ccatatcatt
     2041 t
//
LOCUS       DAXWLB010000246         2019 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXWLB010000246 DAXWLB010000000
VERSION     DAXWLB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.253"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..540
                     /locus_tag="ACGE4H_002754"
     CDS             <1..540
                     /locus_tag="ACGE4H_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFF8366117.1"
                     /translation="RKIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFR
                     MLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESL
                     KMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMS
                     LLDYIILFSVTIFFLSILI"
     gene            551..1966
                     /locus_tag="ACGE4H_002755"
     CDS             551..1966
                     /locus_tag="ACGE4H_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFF8366118.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
BASE COUNT          746 a          244 c          344 g          685 t
ORIGIN      
        1 cgtaaaataa agacatgtat ttatttcatc ttgatttatt cagttctgtt tttagtaatg
       61 aaaattattg atgctatcaa gatagagttt tttactatat tttttgcgac atccttatat
      121 attgtatttc gaatgttgcc ggtcgtaatg atctcatggt tgttggttag tgtctatact
      181 agcaatgaat tattatcctc tttagaaaaa atacatttac cgaaaaaaat aatgttaagt
      241 attacagtag tgatcagatt ctttccgact tatcgttctg aaataagaat gattaaagaa
      301 tctttaaaaa tgagaaacat tcgcttgtct ttttttcagc ctctgcaata tttggaattt
      361 ctgttagttc ccgttcttat acgtgcaact atgattgctg aagaaatgac tgctaatgca
      421 ataacaaagg ggatagaatc tcctgtgaaa agaagttctt actataaaac aaacatgagc
      481 ttattagact atatcatcct gttttccgtt actatttttt tcttaagtat tttgatttga
      541 ggtgaaagaa ttgtttgaat ttaacaatgt ttctttttca tataaggatg ttaaggaaca
      601 agcgcttgtt gaccttaatt tttcaataaa aaaaggcagt cttacattga ttactggttc
      661 ttctgggagt gggaaatcca cactattaaa gttaatgaat ggacttattc caagacttta
      721 tgacggaaaa ttggttgggg aaattttatt tgaaaataac tcgctacaat ccttaacaca
      781 agaaagtata tcaaaaaata ttggctacgt aagtcaagat cctagaggac aattctttac
      841 aacaaataca acgagtgagt tagtattttc gatggaaaac tttggattta caaaagagca
      901 tatggataaa agaatcaatg aattagctga attattagat ctttcaaata tattagataa
      961 aaacattttt tcgctatcaa gtggtgaacg gcagaaaatt tctattggat gtagtttatc
     1021 gctaaactct aaattaatta tattggatga accgtcttca aatttggact tcaaagcgac
     1081 taagaaactt gctgacttac tttgtgattt aaaaaataaa ggatttacta ttgttatagc
     1141 tgaacatcgt ttttactatt tgaaagaatt aattgatcaa gttctatttg ttaaaaatag
     1201 aactgtaaaa actacaacta ttcagttgtt aaaaactgaa aacaataacg cattaaggaa
     1261 atttgacatt tttaatagtt cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt
     1321 attaaaagtt gaagaaatta cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa
     1381 agttggagat gttattggtt tggtagggaa aaacggtgtg gggaaaacaa ccttactaag
     1441 aatactcatg aagattattc gccctaccaa aggaaagata atcgaaaata aaaaaaattt
     1501 gtcatcgccg tttttagtaa tgcaagaaat ggattaccaa tttttcacag aatcagtgag
     1561 gagcgagtta gccctgggga atgaaatagt tagtaaagat cagcaagaaa aaattttaaa
     1621 aaaaatggaa ctttataaaa tgaaagatca attaccgttt gatttatccg gtggagaaaa
     1681 acaaagatta ttggtttcta tagcttcatt gtcaaaaact aatttatttc tatttgatga
     1741 acctacaagt gggttggatt atattaatat ggaaaggatt gctgacctta ttaaagactt
     1801 aaaagaaaag ggtgcagtag ttatagctac tcatgatccg gaatttcttt ataaaacatg
     1861 caatagaatt atttatttaa aaaatggtgg aataaaaaaa gatataaaac ttagagtaga
     1921 agattcaact ttgatagaac agatttttaa tgacataacg aaatagaatt gaggagaata
     1981 tatatgaaaa caaaagatta tatttttatt ggtttagct
//
LOCUS       DAXWLB010000247         2010 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXWLB010000247 DAXWLB010000000
VERSION     DAXWLB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.254"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(60..761)
                     /locus_tag="ACGE4H_002756"
     CDS             complement(60..761)
                     /locus_tag="ACGE4H_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFF8366119.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(777..1217)
                     /locus_tag="ACGE4H_002757"
     CDS             complement(777..1217)
                     /locus_tag="ACGE4H_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFF8366120.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1217..1792)
                     /locus_tag="ACGE4H_002758"
     CDS             complement(1217..1792)
                     /locus_tag="ACGE4H_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFF8366121.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          582 a          420 c          311 g          697 t
ORIGIN      
        1 gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt
       61 tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt
      121 ccttccaatt cagctaattt ttcttggatc tgacggatca ccttttttgc atcttctgga
      181 ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct
      241 tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg
      301 ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa
      361 gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg
      421 tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct
      481 aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg
      541 tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc
      601 aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg
      661 acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc
      721 ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat
      781 aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct
      841 tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc
      901 tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc
      961 gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt
     1021 gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca
     1081 taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc
     1141 aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct
     1201 aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta atagatactg
     1261 ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt
     1321 ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat
     1381 aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag
     1441 aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat
     1501 ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag
     1561 taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt
     1621 ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac
     1681 cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt
     1741 tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac
     1801 actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag
     1861 attttgtagt tttttagtgg tatataggat agacgccact atatatagta tttagaaaaa
     1921 aatcattttt cagccatttt atagataaaa caaaaataaa taacgtgttt tcatcgatat
     1981 gaaatgactt acttatagaa gatactggga
//
LOCUS       DAXWLB010000248         2003 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXWLB010000248 DAXWLB010000000
VERSION     DAXWLB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.255"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..378
                     /locus_tag="ACGE4H_002759"
     CDS             <1..378
                     /locus_tag="ACGE4H_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFF8366122.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACGE4H_002760"
     CDS             426..1826
                     /locus_tag="ACGE4H_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFF8366123.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAXWLB010000249         1999 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXWLB010000249 DAXWLB010000000
VERSION     DAXWLB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.256"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            310..927
                     /locus_tag="ACGE4H_002761"
     CDS             310..927
                     /locus_tag="ACGE4H_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366124.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            968..1267
                     /locus_tag="ACGE4H_002762"
     CDS             968..1267
                     /locus_tag="ACGE4H_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFF8366125.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1301..1468
                     /locus_tag="ACGE4H_002763"
     CDS             1301..1468
                     /locus_tag="ACGE4H_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFF8366126.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1513..>1999)
                     /locus_tag="ACGE4H_002764"
     CDS             complement(1513..>1999)
                     /locus_tag="ACGE4H_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366127.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNPSLDRLGRDYEDIKNTVAFMKQK
                     KVALKILDAQFLDFNTGNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKA
                     GRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKAND
                     L"
BASE COUNT          663 a          324 c          393 g          619 t
ORIGIN      
        1 tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa aatataccat ggaagacata
       61 aaagaaaaac tggataacta gtttatttaa tggcagaccg ttaaggcctg ccatttttat
      121 gtattttagc ggaataaata taatcatgcg ggagtgtaaa cttactgttt caaaaagtat
      181 actttttgac atgccttgat tttctttgtt tatgtatggc ataataacca cataatacaa
      241 tttgaaacgt ttctgattta atctgattgt tttgaaacaa ttttagtgaa aaaagggtag
      301 ataaaaacga tgagaaaatt tttttatatc cgggtcagta gttatgatca atcgatcgaa
      361 cgacaactga tttccgcgaa ggaattagga atccctgaag aatatatatt tgtagaaaaa
      421 gccagcggca aagattttaa acgccctgaa tatcaattaa tgaaacggat gtttcgaaaa
      481 ggagatttac tctatctaca atcattagat agactaggca gaaataaaca aatgatttta
      541 gaagaatggc gagaattaac taaggaaaag cagattgata tttctgtgtt agacatgcct
      601 ctgttagata ctgctagtta caagcaacta gatgggttag agacgcttat ctctgactta
      661 attctgcaat tactcagcta tatggccgaa gacgagcgga aacgaattaa tagccggcaa
      721 agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat tcggcagaaa gaaaattgct
      781 ttacctcctg aatttcctgc gatctattct gactggaaag caggaaagat aactgcagtc
      841 tatgctatgg atcaattggg catgaaacga aatacctttt atcgacgggt gaaagaatat
      901 gaacatacct tgcaccactt ttcttgatag atataacagc tattagacgg gaagggagag
      961 tttgaaaatg gacttaacga atcaaagggt cgtttttact ggaacgctgc agcaaatgac
     1021 acgaacccaa gccatcggtt tggttcactc tttaaacggc cattgtcaat caagtgttac
     1081 ttcaaaaaca aatttcctgg tttgtggtca gtattctaag tcgttgtttg atgattctct
     1141 ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg ttaggtcatg agattaccat
     1201 tttatcagaa gtcgaatttt atgcgttaat tagtcaacag ttgaaagaat ggcaacggta
     1261 tttataatcg ttagtaaaat ttacaaggag gaaaattttt atgaagcgaa aatcttatga
     1321 tcgttggggc attgaaataa ctagttttaa tcgaaatatt gcttttcacg tggagtgtga
     1381 ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac ttttactgtc ctaaatgcca
     1441 tcgccgatac ggggtacatg gaacatagaa acgaaatagt gcggtagaaa agcgaatgaa
     1501 tgtagtatat ttctacaaat cattcgcttt ctttatttta taaatagttg gtcgagagat
     1561 cccgttttct tttgcaatct ctgcaactgg aatgccttgc tttaacatgt ccaccacggt
     1621 tttaaaaact aagcgttttt gcggatctgt tgagttaata gaatactcga ttggtcgtcc
     1681 cttatatcgg ccggcttttt gagccaataa tactccttgc cgctgacgtt cacgaatttt
     1741 ctcacgctcg ttttgtgcaa tataacttag cagtgataaa aacatatcga acatagcggt
     1801 atccaggagt tcatttccag tattaaaatc taaaaactgt gcatctaata tttttaacgc
     1861 tacttttttc tgtttcataa aagcaactgt attttttata tcttcataat ctcttcctaa
     1921 acgatctagt gatgggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc
     1981 caaatatctt aggctgtta
//
LOCUS       DAXWLB010000250         1988 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXWLB010000250 DAXWLB010000000
VERSION     DAXWLB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.257"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            271..837
                     /gene="amaP"
                     /locus_tag="ACGE4H_002765"
     CDS             271..837
                     /gene="amaP"
                     /locus_tag="ACGE4H_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8366128.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            850..1038
                     /locus_tag="ACGE4H_002766"
     CDS             850..1038
                     /locus_tag="ACGE4H_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8366129.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1051..1608
                     /locus_tag="ACGE4H_002767"
     CDS             1051..1608
                     /locus_tag="ACGE4H_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8366130.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1640..1882
                     /locus_tag="ACGE4H_002768"
     CDS             1640..1882
                     /locus_tag="ACGE4H_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFF8366131.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          307 c          406 g          553 t
ORIGIN      
        1 taaatagaat cttgtaataa taactgagca taatctttgc aatttttaat cttgaataat
       61 atcctttaag tatagccagc aatcaaaagt aaatagagaa taagtaagca attgttattt
      121 cagtatcaga ctggcaataa aacgccgtta tgatccagca atgtttggta gcgctttatt
      181 tactgatgct atgctaactg ttcgctatct atactgtgat tgtcggtaga gttatttaca
      241 gggtaaaaat aagtttatag gaggtggaaa atgaataagg ggttaaaaat aatcggtgtt
      301 atcatgagtt tactgctact ttcagtattt ctagcgattg cgttgatcaa tgcgccgtat
      361 attatgcctg aacaattgga acgatttcgc ttctttactt taacgaatta ctacatgcaa
      421 caatatattt tctgggcagc tgtagtattt gccgttcttg ctattatcat cttgctagtc
      481 gttcttttct atccaaagtc aagaggaact ttcgttatga aacgggaaga tggcaaacta
      541 acaatcgaca aaaaagcgat cgaagggttg gtacgttccc atttacacga agaagagttc
      601 attcattcac ctaaagtacg gatacgttca acgaaaaatc gaatccatat ccatgtcaac
      661 ggagacttga aacgaacttc ttcattagta gggaaaacag gtgtattgat gcaagatatc
      721 gaagaagaag taactaaagt actcggaaca aaagaaacgg tcaaagtggc agtcacatac
      781 agcggttatg aagaacaaga agatacaaga gactacaagc actctcgagt ggaataggag
      841 ggccaaataa tgaaagaact attttcagca tacaagctcc ctattatctt tgggggtctt
      901 ggactactgc tagctgtttt atggttaacg attggatttt tcaagacatt gttgatcata
      961 atcatgacag caattggcgc aggaatcggt ttttatttga aacagtcagg aatagttgat
     1021 caatttttta aatcatagga ggaaacaaag atggaaaaca aaaacatgaa tacaacaaat
     1081 ggatcagttt tggcaaatga aaaaggagaa aaccgcatga tgaatggtga attgacttat
     1141 gaagacaaag tcatccaaaa aatcatcggt tatgcattga aagatgtaga tggtctattg
     1201 acattagatg gcggattttt ctcaaatatg gctgaaaaat tagtcaacac agataacgta
     1261 acagcaggta tcaatacaga agtaggtaag aaacaagtag ctgtggatct agatgtgatc
     1321 gtagaatacg gtaaagacat cgaagatatt tacaaccaaa tcaaagaatt gatcagtact
     1381 gaagtaaaca atatgacaca tttagatgtt atcgaagtga atgtgaatgt tgcagacatc
     1441 aaaacacaag aagaatacca aaaagatcaa gaaacagtac aagacaaagt aacagaagca
     1501 gcaaaatcca caggacaatt tgcttcacgc caaacagata aagcaaaatc tgctgtaggc
     1561 aaaggtgcac aaaaagtcaa agaaaataac gaaccacgtg tacaataaga agtaagaagt
     1621 cagttaggag gaatttaaaa tgggatttat ttgggcatta atcgtcggcg gtatcattgg
     1681 agcaattgca ggagcaatca cgaataaagg atcatcaatg gggatcatcg caaacatcat
     1741 tgctggttta gtaggttcag caattggaca agctttacta ggaacttggg gaccaacaat
     1801 tgcaggaatg gctatcattc catcattgat cggtgcaatc attttagtat tagtcgtttc
     1861 attctttgca cgtaaattat aagcaaattg aagaaaaact tatgttgaaa tgaagaacaa
     1921 atttgagaac cgtagcgaca ctcgctgcgg ttctttttta tatattttat atgaagtatt
     1981 ctttattc
//
LOCUS       DAXWLB010000251         1955 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXWLB010000251 DAXWLB010000000
VERSION     DAXWLB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.259"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4H_002769"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACGE4H_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFF8366132.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          700 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaa
//
LOCUS       DAXWLB010000252         1950 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXWLB010000252 DAXWLB010000000
VERSION     DAXWLB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.260"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            286..882
                     /locus_tag="ACGE4H_002770"
     CDS             286..882
                     /locus_tag="ACGE4H_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8366133.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(940..1422)
                     /locus_tag="ACGE4H_002771"
     CDS             complement(940..1422)
                     /locus_tag="ACGE4H_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366134.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1528..1725)
                     /locus_tag="ACGE4H_002772"
     CDS             complement(1528..1725)
                     /locus_tag="ACGE4H_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366135.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          677 a          377 c          286 g          610 t
ORIGIN      
        1 aaaagcaagt aatttttaat ggcaatatca taaaaaaaag aagatagaga tttaataatt
       61 atatatcagc tgtttttttc aaataagcaa aaattcctaa aacacaacaa tgaaagcgat
      121 aactaagaaa atccttacat aacgattttt cttattaaaa ttgttttttg aacttcgctt
      181 attttttgtg agcatatctg tttacttact ttttgtaagg tgctatactt catttcgtaa
      241 tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt ttattatgac aacttttact
      301 gacactttga aaaaccgccg ttcaatctat catctaggac gtaatgtttc actttctaat
      361 gaagaattaa caactttgat caaagaagca attaaagaaa gcccaactgc tttcaatgct
      421 caatcaacac gcgcagttat ccttttcgga gatgcacatg aaaaactttg ggaaatcact
      481 gaagaagcac ttcgcccatt gactccagca gaagctttcc caaatacaca aaacaaatta
      541 gctggtttca aaaatggtta tggtacagta ttattcttca aagatactga cgtggtaaaa
      601 ggtctacaag aacaatttga attatatgca gacaacttcc cagattggtc agaacaatca
      661 aacggaatcg caacagctaa tacttgggta gcattagttg ataaaggatt gggcgcaaac
      721 ttgcaacatt acaacccagt tattgacgaa gcagttgcaa aagaatggaa tatcccttca
      781 aactggaaac tacgttcgca attagtattc ggttcaccag aaacaccggc tggggaaaaa
      841 gaatacatga acgatgccga tcgcttccgc gtatttggat aattttattt gaaataaaaa
      901 acagaggttg tgacaaacat cttgtcgcaa cctctgtttt tattctggat caatcataat
      961 atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat ggaactaatt cttctaatga
     1021 tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg aaaaaattca tttttacgat
     1081 tttagaacct tttggctgat ataatacaat tttattgatc tcagaaaaag gaacaccttt
     1141 tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt cctctactgt ccaacaaaaa
     1201 actaataaaa atcattgctg acaagatccc ttttgccaat tcatctgctg taggatgact
     1261 gatgagtgta taccaaatga ctagtgacac taagaagggt atcaccaagt agacccatcg
     1321 atttcgcaca gtccgataaa taatttcttt tcgaaccagc caaagaaata aaaacatcac
     1381 aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc atgacaactc gcctcgcttt
     1441 ttcttctcca gaaaatagga aaagaatact gatttacctt ctcaaagaaa ttggacttgc
     1501 agcacgccgc acgtccaatt gttttcttta ttcaaatgta tcatattttg caacaaccat
     1561 gcttgctttt tcatctaata attcttggtc ctctgcagca cattgggcaa ctttgaccac
     1621 ggcgcagttt gtcatttttg atacaacttc accaatgacc gtttttgtaa aaccaattgg
     1681 tttgcatgca tattgttctc ctaaattaat catttcttcc gtcatcaaaa tcctcctaaa
     1741 ctgctggaac ttttctccac attaccactg ctatttcttt ggagatttaa ctcattgatt
     1801 tttatttaca gttaaaaatc aaccttacag gcattatagc gtactgtttt cgttttcaca
     1861 attaaaacag ctgttgtatt tagaagaatt tttttcaaaa aattaaaaca tattccttaa
     1921 aaactaatta tttcctacat attaaaatat
//
LOCUS       DAXWLB010000253         1950 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXWLB010000253 DAXWLB010000000
VERSION     DAXWLB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.261"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(50..241)
                     /locus_tag="ACGE4H_002773"
     CDS             complement(50..241)
                     /locus_tag="ACGE4H_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366136.1"
                     /translation="MNTLKDDFILAKAGSEEAIESILKRFSSLMHQQSWRNGRYDQDC
                     YQECMIAVYLAISKFEIKE"
     gene            complement(315..701)
                     /locus_tag="ACGE4H_002774"
     CDS             complement(315..701)
                     /locus_tag="ACGE4H_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFF8366137.1"
                     /translation="MSENDERVNAQLICFFEKVIKNTAYSYFERQKKIRELEQFDQFS
                     QHFFTENNIKIKQPVTILDVTFLIEDSRLAEIIPLLKEREQLFLIEKFVFDKTDKEIG
                     ESFGITRQGITNLKHRLYRKIQKLLI"
     gene            complement(762..1223)
                     /locus_tag="ACGE4H_002775"
     CDS             complement(762..1223)
                     /locus_tag="ACGE4H_002775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366138.1"
                     /translation="MKLEEEKDAIYTQMRMILEERRRLSKDYYELKARLFALDNTNQP
                     NSLIRNEKLAKKSSTNISEEALYQQYICERGNKYRQLIPYSTISLTISSILKDAGRPL
                     SNKEIYKLLISDHKLNISYKNLTHNILPKMNVDNSVNVERAYRGYWQYRLH"
     gene            1447..>1950
                     /locus_tag="ACGE4H_002776"
     CDS             1447..>1950
                     /locus_tag="ACGE4H_002776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366139.1"
                     /translation="MFNQKKEKQFKYAIKKKKGGGGAASFVIGAVFLGIGLISYPTLS
                     FASETTEINQSSEIQSSLTNSVLKESNSEEKQNIDMSEDSSINNDLELDSKATVSGSE
                     NSFDETTNNLETTNLSEDSIEVQSESIIQNEDSTDNDAVQELEMNPHEDNGDDISVEN
                     QMTEATEE"
BASE COUNT          668 a          288 c          295 g          699 t
ORIGIN      
        1 tttttaaaat cgataagaaa gagagaagca aaaggcctct cccttgattt tattctttga
       61 tttcaaattt tgatattgca agatatactg caatcataca ttcttgatag caatcttgat
      121 catatcttcc atttcgccat gattgttgat gcatcaaaga actaaatctt ttaagaatag
      181 actcaatagc ttcttcactt ccagcttttg ccaaaatgaa gtcatctttc aaagtattca
      241 tagaccgtcc tcctttccta aatttaaagt tatttagagg aggtggtcgt atacaaataa
      301 tcgttaagtg gcttttaaat aagtaacttt tgaatttttc tgtaaagacg atgttttaag
      361 tttgtgatgc cttgtctagt gatcccaaaa gattctccga tttccttatc tgttttatcg
      421 aaaacaaatt tttcaataag aaatagctgt tctctttctt ttaataaagg aataatctct
      481 gccaatcttg aatcttcaat tagaaaagta acgtctaaaa tagtaactgg ttgtttgatt
      541 ttgatattgt tttcagtaaa aaaatgttga gaaaattgat cgaattgttc cagttcacga
      601 atcttttttt gcctttcaaa ataagagtac gcagtatttt taataacctt ttcaaaaaaa
      661 caaataagct gtgcattcac gcgttcatca ttttctgaca ttctgctcac ttcttccaat
      721 aaatatttaa aaggaaaagg gacagaaagc gaaaaaagaa gctaatgcaa tctatactgc
      781 caataacctc tataagctct ttctacattg acactattgt caacgttcat tttgggtaga
      841 atattatgag ttaagttttt gtaagaaata ttaagtttgt gatcgcttat taatagttta
      901 tatatttctt tatttgataa tggtcgacca gcatctttta gaattgagga aattgtcaaa
      961 ctgatagttg agtatggaat caattgtcta tatttatttc ctcgctcgca aatatattgc
     1021 tgatacagcg cttcttcaga aatatttgtt gacgattttt tggccaattt ttcatttcta
     1081 attaaactat taggttgatt agtattatct aatgcaaaaa gtctggcctt tagctcataa
     1141 taatctttac taagtctcct cctttcttcg agtatcattc tcatttgtgt ataaattgca
     1201 tctttttctt cttctagttt catctacttc tcctttcatg attaacaact aaaaatattt
     1261 ttaaaaggac acaaaatagt atattattac ataattaaaa atgagcgctc atattgaaat
     1321 tttttctaaa ctgatatact attaatagta tatgacgaag caagtttatt ttctttgttt
     1381 tcataaaatg aacgattatt tcgtctcttt ttgattttta tactgtaata aataaggagg
     1441 aaaattatgt ttaatcaaaa gaaagagaag cagttcaaat atgcaatcaa aaagaaaaaa
     1501 ggtggcgggg gagcagcttc ctttgtcata ggtgcagtat ttcttggaat agggttaatt
     1561 tcttatccta cattatcttt tgctagtgaa acaacagaga taaatcaatc aagtgaaatc
     1621 caatctagtt tgactaattc tgttctaaaa gaaagtaatt cagaagaaaa acaaaatatt
     1681 gatatgtcgg aagatagttc tataaataac gatctagaac tagattcgaa agcaaccgtt
     1741 tctggatcag aaaattcatt tgatgaaact actaacaatc tagaaactac aaatttatct
     1801 gaagatagta ttgaagttca gtcagagagt attattcaaa atgaagactc gactgataat
     1861 gatgctgttc aagaattaga aatgaatcct catgaagata acggtgatga tatttcagtt
     1921 gagaatcaga tgacagaagc tacagaagaa
//
LOCUS       DAXWLB010000254         1932 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXWLB010000254 DAXWLB010000000
VERSION     DAXWLB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.262"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(312..385)
                     /locus_tag="ACGE4H_002777"
     tRNA            complement(312..385)
                     /locus_tag="ACGE4H_002777"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(349..351),aa:Ile,seq:cat)
     gene            complement(574..1593)
                     /locus_tag="ACGE4H_002778"
     CDS             complement(574..1593)
                     /locus_tag="ACGE4H_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8366140.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1604..1810)
                     /locus_tag="ACGE4H_002779"
     CDS             complement(1604..1810)
                     /locus_tag="ACGE4H_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFF8366141.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          568 a          394 c          328 g          642 t
ORIGIN      
        1 ggtatgcctt ttaatagctt caaatataaa agagtttaaa gcgtaacaca cttatgggga
       61 agtggcttgg ggtgcgcttt aaactcttct ttattatcat ctcacaattt aacccaaatg
      121 tctttctatt taaaaatcaa agtaaaactt ttcaaatata cagaaatata actatgtgaa
      181 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
      241 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaaat
      301 ggctacgtta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      361 ctctgaccaa ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc
      421 agtagaatgt gtggcaacaa acctgttatc gcatagcttg gagtgtgact attatgggtg
      481 atagtgaaga tatgcgataa catcactatt ttatcgaatg atttttatag ttgtcaatat
      541 agttatgtaa tgctcctcaa cgaggagcta tttttatcgt ttaggaatat ttaaatacca
      601 acgtttgtca tggaaatctt gcgcaccgcc tttagtgttt ccctctggat cattcgttgc
      661 ccgcatcatg acatagactt tcttattagg gaagttacgc atgttaaaag atacatgata
      721 gccaacattt ccagaagtat tataagcttg atttacatct gatctataaa ttccatcagc
      781 tcttactcga gctaattctt tcccagtatt gtaatccata atgaagatat actcgtattt
      841 atagttagca atgtgccatc cagctacatg caaatttgca ttttcgattt ctccaaactg
      901 atcaatgtgg gcgtaattcg ttccatctgt cagtgtagga ttagctgcac cagctcgtgt
      961 tggatcaatg acaggcttgt tttcagaagt tgttggattt tcatcggtaa atccatgagc
     1021 taaatcatat gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc
     1081 tgtatgatcg ccccagatgt tttgtgttac ccataaatgc gatttgattc ccggttggtt
     1141 ataaggagtg tccaacgtta atggaatacc atatttcatt gctgaatctc tagccaattc
     1201 aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt gtaactcaat
     1261 ctgcacagga ctgttggcat tagcatacga accagcaccg tactgtacat aaccaggttg
     1321 accgacttga taaacaattc cgccgtctcc cacaatataa gcagtataag cgctagtcca
     1381 tgaacgttgc atatactgcg cttcattgcg tcctgttgct gtttcattag ccgtttcatg
     1441 cagtaaaata tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa
     1501 ttcattgttg atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag
     1561 gctcatcgca gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa
     1621 ttttcttata cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg
     1681 taataatcat caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga
     1741 caaaagtcgc taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc
     1801 tattgttcat actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg
     1861 ttttcttcta tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt
     1921 tcaattttat ct
//
LOCUS       DAXWLB010000255         1881 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXWLB010000255 DAXWLB010000000
VERSION     DAXWLB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.263"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            129..1853
                     /locus_tag="ACGE4H_002780"
     CDS             129..1853
                     /locus_tag="ACGE4H_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFF8366142.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          740 a          233 c          334 g          574 t
ORIGIN      
        1 tcagtttgta acaaacatat attttttata ttacaatatt tcaacaaatt aaaaaaaatt
       61 atatcttctt aaacaatgat aatattaaaa tgcaagcgct tgtaaaaaat aaggagaaga
      121 ggtaaaaaat gaagaaaaaa ctattaactt tatttcttgt gatgtctttc tcaatcgttt
      181 tgagtgtgta ctacaatact attgtttttt cagaacaaat aaactacaca gaagaacagt
      241 ataatataga aaaagaaaaa gctttaaaag ttggttatag tgaagaacag tttaagcaga
      301 ttatggaaat acctacaaat ttatcaaatg aaaatagtga aacaagaata gtaaattata
      361 ctatgacttc taatcaaacc aaagtcatta ataaagcaat ggagcaaatt ggaaagccat
      421 acgaatgggg agcatcggga ccaaattctt ttgactgtgg tggtttagtg aaatatgttt
      481 acaaacaagc tgtaaatatt gaactgccta tgggaaccac caatcaagag caatacggta
      541 cagaggtaag tttaaattca cttaagcctg gtgatttatt attttacggc aatcgaggtg
      601 caacttatca tgtaggtata tataaaggta acggtgtaat gattcatgca ccgcaacctg
      661 gagaaactgt aaaagaggtt aacatacaat atttttatcc taactttgca aaaagaattt
      721 tgcctgatga acctgactac ccttatattg actataataa aatggtaact gttacaaaag
      781 cttggtcaat atggaatgat ttacaattta gtcatgaaat taagaaagct attattggag
      841 ataattacaa gatagggaag gtttatacta atccagaaaa taataataaa tatggagaaa
      901 tattagtaag taataaagta tatggataca ttaattttta tgctgttaaa gagttgacct
      961 ctgttcaaat aaacagatat ttaacttcta aagatagtgg tcaaccaact tggggaaatc
     1021 tagagtgtac tataagtaaa ggacaaacta caaaagataa aatatatttt gttaaaggag
     1081 catataattt aggggatgga aagtatctat attctattta taaagatcag gattcctctg
     1141 aatggttagg gtatttaaaa gcacatgtta gtttagccta tactcctatt gaagaaatta
     1201 ataaaaacgt aactgttact aaaaattggt cgatttggaa taatctacaa tgggaaaagg
     1261 aaattgaaaa gcctcagatt ggttctgtat tttcagctag attaaagtta accaatgtat
     1321 cgaataatgc tgtctattac aagctttata aatctggaaa attttatggt tatatcaatg
     1381 ctgaggctgt caaagacctt acaacaacaa agcttaataa gtatgttaca tttagtgtta
     1441 ataatgaaga tttttggtct tctttagatg taaattattc aaaaggaaaa actgaaagag
     1501 gccgagtatt ttacattagt atttcatata ataccgcaga taaccaaccg atttattcag
     1561 tatatacaga tgaaacatgt acggaatggc gagggtatta taagggaaac aactttgaag
     1621 acactcaaat tactatgctt gagaataaaa gcgtgaaagt aacaaaaagt gggtatacag
     1681 tatggggaga cttgaatttt tggactaagt caggaatatc gaacacagga gatatataca
     1741 ccactgaccg aaaattttat aattttacca acaatgctta ctattatgaa ttgaaaaaag
     1801 atgggagggt ctatggctat ataaatagtg aagcagctgt agagatgaac taaaatattg
     1861 ctgtccctta atcttatggg g
//
LOCUS       DAXWLB010000256         1847 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXWLB010000256 DAXWLB010000000
VERSION     DAXWLB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.264"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(95..604)
                     /locus_tag="ACGE4H_002781"
     CDS             complement(95..604)
                     /locus_tag="ACGE4H_002781"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFF8366143.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(716..1714)
                     /locus_tag="ACGE4H_002782"
     CDS             complement(716..1714)
                     /locus_tag="ACGE4H_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFF8366144.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT          560 a          356 c          328 g          603 t
ORIGIN      
        1 ttgctgtttt tcttttctta ggaaaaaata gcttgaaaca aacaaaaaag aaagaactcg
       61 tctaaataga cgttttcttt ctttttttgt tgaattattg agtaaggcga cttctttttt
      121 ctggttcttt gtgtgctaat cgactggcag cagcagcggc gatcgctccg acgatatcat
      181 ctaagaaggt atggacatga acgccatcgt ggctgtttag ttcttttaaa ataccaggtt
      241 tcaccttgtc gatataacca taattcgtaa agccgatcgt accgtaaaca ttcacgattg
      301 acaaggctag gatctcatcg ataccgtaaa ggccttcgtc atcttcgata atatgttgca
      361 acggactcat caattttttc ttttctgcta atatatctaa ttggattcca gtgataatgg
      421 cgttatgtac ttcacgcttt gttagcacgc tgttgacact ttcagtacaa tccgccaaag
      481 tcaaatcaga aatatagtct ttttgtaaaa acatgacaag ttgtgcgata tcttctacag
      541 ttacgccacg ttccttcaat agttctcttg ccttttcttc taaagtctca gttttgacga
      601 ccatattatt gctccccctt tattttttct ttctctattt catgttaacg gaacatgaaa
      661 aaaatgcaag aaactgcttt cgggtttaac ttgaaatgca aagagatata aaaaatcaac
      721 taggacgaag atttttatcg tataagcttg tgctgtgacc aggctgagtt cgttcctttg
      781 gattaatata gtgtaaagcg ttgttcactg cagtaggtgc ttcaccgagc ccagtagcaa
      841 tcagtttcac ttttccttca taggtacaga tgtcaccagc tgcataaacg cctggaatag
      901 aagtagacat atctgatttt acagtaatgg catttctggt actatcaagt ccccaagaag
      961 aaagatgttc taaattagaa gagaatccat aattgacgat cagagaatca atcatcaaat
     1021 caatcgtctc gtcacttttc acttttttta accggatatc tgtcagttcc ccattattgc
     1081 ctgataatcc atcgatgaga tagggagtaa gcaagttgac agatgaagat ttcaagcgtg
     1141 atacactatg ttcgtgcgca cgaaattccg ggcgacgatg aatcaagtaa acttcgcttg
     1201 caataggctc taacatcaac gtccaatcga tagcagaatc accacctcca gcaatagcga
     1261 ctttttttcc agcatacttc atcaaatcat tcacgaaata atccaatccg tggttttcaa
     1321 atgattcagc attatctaaa ttcaattttc ttggctggaa tgaaccattt cctaaagcta
     1381 aaatgactgc tttggaataa tggatgcctt tatttgtagt aatctcgata acttcgtctt
     1441 cacgagtaag cgagagaact tcttctttta aatggaatgt atggttaaaa gtagttaatt
     1501 gtttttctaa ttgatcgatt aattcacttg cttttattgc tggataccca ggaatatcgt
     1561 aaatatattt ttcgggatac aaagttgcta attgaccacc aagttgaggt agactgtcaa
     1621 tgatttttgt tttagcttgg cgcattcctg cgtaaaaagc ggcaaacatc cctacaggac
     1681 ctgcaccgat gattgtaatg tcatagattt ccataaaaat tcctcccttt tcacactata
     1741 gcagaattta attgagaatg cttctcaaaa atgtttgaat tcgaaatcga aacggtaagt
     1801 tatagaatgt atctagtgat gaaaaataaa agaaaacaaa tctagta
//
LOCUS       DAXWLB010000257         1835 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXWLB010000257 DAXWLB010000000
VERSION     DAXWLB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.265"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..722)
                     /locus_tag="ACGE4H_002783"
     CDS             complement(<1..722)
                     /locus_tag="ACGE4H_002783"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8366145.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="ACGE4H_002784"
     CDS             complement(727..1494)
                     /locus_tag="ACGE4H_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFF8366146.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
BASE COUNT          591 a          290 c          308 g          646 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaac
//
LOCUS       DAXWLB010000258         1805 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXWLB010000258 DAXWLB010000000
VERSION     DAXWLB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.266"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>1805
                     /locus_tag="ACGE4H_002785"
     CDS             <1..>1805
                     /locus_tag="ACGE4H_002785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366147.1"
                     /translation="IKSNTQEVDKDATVLSDTPRDLVNLEDTSTDIENVTPQKIGKKD
                     STSLSEENLSSPASSILRRRTRSLDVQTGETFLEDWALPDNSNISGSLITQTGITYVG
                     VKTDDPTQLHLKMAYRAAATPAAIDWIYIRVDPELTKYIEEINIKNTVNIGAQVGYQR
                     YFEKVDANTLSDVNTFGTPTGVSDAYAQVDQTTGGIYRVLLDKFFTRIPGVKPKERGA
                     LSGNWGVFPSVPLEAERYEANIYIKLSKDVAELQQELGRDKFGVQLRLQGQSSSGVAT
                     IAKESLNKNTIIDLSKQESIENNSNWLNSPSVHTITTTYEGKQMGSDGQFSNTLEGTD
                     KSLIRVQNTIMNNLLYGTALDLGRPVSFNIEIDPQILNLVSDDDYITIWQVYRENSIN
                     HHDGGSNAPVMLKKNMFVDNKIKIEVNKDKAGQYENGVYYVNAVESFMNTVSSSPFVG
                     ANFIDIPVMPKPLIEDEGQLVSAEIKTWFTDKKNDQERVVRNSLASSYINLFPTTINF
                     YDYSTKDTEQLLKLRESTYLVSTFGQEINGMDMGETYEISNYEFVTADPESLIITQKD
                     GNVINLYYKKLSSDAEFYEPEVEKEEVNYGGTIDLT"
BASE COUNT          656 a          253 c          353 g          543 t
ORIGIN      
        1 ataaaaagta atactcaaga agtagataaa gatgcgacag ttttatctga tacaccaaga
       61 gatttagtta atttagagga cactagtaca gatatagaaa atgttactcc acaaaaaata
      121 ggtaaaaaag acagtacgtc tttatcagag gaaaatcttt cgtcaccagc atcaagtatt
      181 ttacgcagaa gaactaggag tttggatgtt caaactggag aaactttttt agaagattgg
      241 gcactaccag acaattctaa catttctgga agtttgatta ctcagactgg aattacttat
      301 gtgggtgtaa aaactgatga tcccactcaa ttacatttga aaatggctta cagagctgca
      361 gcaacaccag ctgcaattga ttggatttat attcgtgtgg atccagagct tacgaaatat
      421 attgaagaaa ttaacataaa aaatactgta aacatcggcg cgcaagttgg ttatcaacga
      481 tattttgaaa aagtagatgc taatacattg agtgatgtaa atactttcgg aaccccgacc
      541 ggtgtaagtg atgcttatgc acaagtagat caaacaactg gcggaatcta tcgtgtgtta
      601 ttagataaat tttttacacg tattccaggt gtaaaaccta aggagcgagg tgcgttatct
      661 ggaaactggg gtgtatttcc atcagttcct ctcgaggctg agagatatga agcaaatatc
      721 tatataaaat tatcaaaaga tgtcgcagag ttacagcaag agcttggaag ggataagttt
      781 ggtgttcaac ttcgtttaca aggtcaatca agttctggtg ttgcaactat tgctaaagaa
      841 agtttaaata aaaatactat tattgatcta tcaaaacagg agtcaattga aaataattca
      901 aattggttaa actcaccatc tgtgcataca attactacta cttatgaagg aaagcaaatg
      961 gggagcgatg gtcagttcag taatactctc gaaggaactg ataaaagtct gattagggtt
     1021 cagaatacaa tcatgaataa tcttttatat ggaacggcac ttgatttagg tcgacctgtt
     1081 tcatttaata ttgagattga tccacaaata ttaaatttag tttcagatga tgattatatt
     1141 acgatttggc aagtatacag agaaaatagt atcaaccatc atgacggtgg atcaaatgca
     1201 cctgtcatgt tgaaaaagaa tatgtttgta gataataaaa ttaaaattga agtcaacaaa
     1261 gataaagcgg gtcagtatga aaacggagtt tactatgtga atgcggttga aagttttatg
     1321 aatactgtga gttcctctcc atttgttggt gcaaacttta ttgatattcc tgttatgcct
     1381 aaacctttaa ttgaagatga aggtcaattg gtatcagcag aaattaaaac atggtttacg
     1441 gataaaaaaa atgatcaaga gagggtagta agaaactcac ttgcttcaag ttatattaat
     1501 ttattcccta ctactattaa tttctatgat tattcaacca aagatacaga acaattattg
     1561 aagttacgtg aatcaaccta tttagtttct acgtttggtc aagaaattaa tggaatggat
     1621 atgggggaaa catacgaaat tagtaattat gagtttgtga ctgccgatcc tgaaagttta
     1681 ataattacac aaaaagatgg aaatgtcatt aatttatatt ataagaaact ttctagtgat
     1741 gctgaatttt atgaaccaga agtagaaaaa gaagaagtca actatggagg aacgattgat
     1801 ctgac
//
LOCUS       DAXWLB010000259         1779 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXWLB010000259 DAXWLB010000000
VERSION     DAXWLB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.267"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            152..982
                     /locus_tag="ACGE4H_002786"
     CDS             152..982
                     /locus_tag="ACGE4H_002786"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFF8366148.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            954..>1779
                     /locus_tag="ACGE4H_002787"
     CDS             954..>1779
                     /locus_tag="ACGE4H_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8366149.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQK"
BASE COUNT          652 a          225 c          360 g          542 t
ORIGIN      
        1 cttcaaacaa tcaaaatctt atttgataag atgtcctcac aaggactaga tgaagatgaa
       61 aatatcagtt cattagcaga aagtgcattt ccaaaagaaa aaacgtatcg ttagattgga
      121 aaaattttga gcaaagaagt aggagagaac attggaaaat aaaaatataa gtatactgct
      181 ttctttgtat gataaagaaa agccggaata tttaaaggaa gcattagcta gtatctttga
      241 gcaaacggtg atgccggcag aaatcgtatt ggtttatgat ggccctataa atgagaagct
      301 tcaggcagtt gtcacttatt tccagcaaaa agttcctcac ttgtttacag ttgtaaaatt
      361 agaaaaaaat caaggattgg ggattgcttt agcaactgga ttagaacata ctaaaaatcg
      421 cttagtggcc agaatggata cagatgacat catggaacca cacagattgg aaaaacaatt
      481 agagatcttc caagaatatc cagaaatagc gattgttggg agtaatattg aagaatttac
      541 tggcacactt agcaatgtta tcggaagaag gattgtacct gagtataatg atgaaatccg
      601 tgtgttttcg aaaagaagaa acccttttaa tcatatgaca gtcatgtttg ataaaaatgc
      661 tattttagaa gtgggaaact atcgcccatt aattggattt gaagattatt atttgtgggt
      721 aagactattg aaggcgggat ataaaggata taatatccaa gagattctag tacatgctag
      781 agcaggtgct gacatgtatg ctagacgtgg aggaagtaaa tatcttttac caggaattaa
      841 aggccgatat cgcgtatgga aagatggtct aggatcacta aaagattttc tgatagttat
      901 ttgcggacat cttgtaatta gtttattacc gaataaaatt aggggaaaag tatatgaatc
      961 aaaacttaga aaaacaaact aaactttcta taatcatacc tttttacaat gcaaagaata
     1021 ctttaagaag agctgtagac agcgtcttaa cgcaaggtta tcccaatatt gaaatcttgt
     1081 taattgatga tggatctaca gatggtagta gtgacattgg tgacgaaatt gctaatctag
     1141 agagtaaagt agcatgttat cacttggaaa acgctggtcc atcagttgct aggaactatg
     1201 ggataaaaga agcaactgga aatttggtag gatttttaga tagtgacgat tattttttac
     1261 ctgattgttt taataatgtt ttgaaagact accataggaa tcaccccgat atcattgctt
     1321 ttggattaag taaaggaaaa aatatagaaa atgcaaattt atttgttccc gaaaagaaga
     1381 ttggttgttc aagtgaagaa gcaattgagc aaatgtttag aagtaaggca gtggactttt
     1441 acgtatggaa taaatttttt aaaagggaat tattcaatca tatagttttt ccagaaaata
     1501 aattatatga ggatatggtt cctatgtatg aagcttttag ggcggctagt aaaattgata
     1561 ttttgccatt ttcaggtatt ttttattatc aaaataacga tagcatcgtt tatcagtctt
     1621 ttaattcaaa acagtatgat aatattttgc agagaaaaat attagttgat gaagtaaaga
     1681 aagattatcc tcgacttatt aatttggcca atgctcggtt agtagacgga tatctttcaa
     1741 cagggtttaa aattactggc aaaaatcaaa atcagaaag
//
LOCUS       DAXWLB010000260         1743 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXWLB010000260 DAXWLB010000000
VERSION     DAXWLB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.268"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(112..1152)
                     /locus_tag="ACGE4H_002788"
     CDS             complement(112..1152)
                     /locus_tag="ACGE4H_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFF8366150.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          582 a          266 c          217 g          678 t
ORIGIN      
        1 ttctattaaa atagctatta ttttgtaata ttcgtaatat aattagaata ataaaaaatt
       61 tttaatttta atgttactcc atgaagcaac aatcactttg tcagtaaagt ttcactctaa
      121 ccagtctttc gtaatagtgc ttggggagct tgcataattc aaacgttttt cacttgatac
      181 tttagcagct attggttttt cgataaacca attttttaca tagacaaaaa tcaggttctg
      241 gatattttcg actttttcgg tttttaattt ttgataggct tttaacagtg tcgtgtataa
      301 ttcttgttct gcattgacat aataactatc gagttcttca aaaactatcg aaatatcact
      361 ttctatttca gcttttttct ttgcattatg aatagtctgg acggtttttt gagcttcact
      421 gtaacttgaa gaaaatgttt ttatcaaatt taatactcgt tcagggacaa acgtagcaat
      481 gctgtcgttt gtcataattg tctcaaaatt gtctaacaac aattgatctt gtaggcggtc
      541 tttttcagtg tctatattgt gtctattagt gtctaatata ttattattaa gttctggtat
      601 gattttcata ccctctgatt tatcaacgtt ttggcacggt atgattttca taccctcttg
      661 agccccaagg gattcagcgt ctgatttttc gtcgagggtg ggtatgattt tcataccctc
      721 tgatttatca acgttttccc tattctctct atcaaatttt tcgagtaagt agatatcatt
      781 ttctgagact tttaattctg ctagatatag tctattagga ttatttttcc ctaactgttt
      841 gttaaatccc atctgctttt gtaataataa accaaaggat tccaactctt ttttgattgc
      901 tatcgctttt tgtttttggc agtttaatac tctacatagt tcatttgtcg tataagtaaa
      961 ataaacatta ccttcttcat cgatttgccg tttgctgatt gcgatctcta atcttgcttt
     1021 taaaagcata taggcaatct tagttgaatc tttcattttt ttgtattcgt cagaaacaat
     1081 aaagactttt ggaaattgat aaaataattc gttgtaaact ctatccgcct taaaaaattt
     1141 aaaatcagtc attgtagatc ctctactttc acgttgtttt atttatgtaa aattacctac
     1201 tcttttcgaa aatagcaaag ataaataaaa aatgcaaacg cattaaaact gttttttaat
     1261 taaatactac ctaaaatcag aaataaaatg aattatgtac tacctttaaa gtcattaaga
     1321 actactttaa ataaatatta tttataatta aatactacca gaaaaacgta tgaataaaat
     1381 ttagttttaa aattaaagat aattaagtac taccagtgtc aaaacaaaaa ataaattaag
     1441 aactacctta tgcatataaa acatactacc attactagta atatatacta ccttatttca
     1501 gtagtatata ttatattatt cttataagac aaggctttag gctagaaaac aatagattat
     1561 atactactaa aaaatacaat aagtactacc aatgtcagat atatataata attatgtact
     1621 accattaaaa ttaacttatt taataagata ctactgtgtt aatctgaaaa aaattacttt
     1681 aaagtagtta catactacct gttaatttag atactaccat taatacttaa attagatact
     1741 acc
//
LOCUS       DAXWLB010000261         1712 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXWLB010000261 DAXWLB010000000
VERSION     DAXWLB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.269"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..506
                     /locus_tag="ACGE4H_002789"
     CDS             <1..506
                     /locus_tag="ACGE4H_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8366151.1"
                     /translation="LQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTIIQVPEIVMT
                     LLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRINSIKKRHPH
                     LVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLVDKAVFEAFQ
                     RGLQKSN"
     gene            complement(664..1281)
                     /locus_tag="ACGE4H_002790"
     CDS             complement(664..1281)
                     /locus_tag="ACGE4H_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366152.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT          566 a          314 c          244 g          588 t
ORIGIN      
        1 ctttgcaatg gaaatacatt gattttgaaa accaaacagt ccgtcttaaa tatgccttgg
       61 ataaacacac aaaaaagaaa tttacaaaag gtcgaaaaga tactattatc caagttccgg
      121 aaattgtaat gacactattg caagaatgga aatcagtgca agctgaacaa ctattgaagt
      181 tgaaaattaa acagacacct gaccaatatc tttttagtta ctccaaacct tctggtgaag
      241 taaattgtcc agtccatact gattacttaa attatcgaat taattctata aaaaaaagac
      301 atccccattt ggttcattta actcctcata aacttcgaca tacttacgca actttagcta
      361 gacaaggtgg ggctgatatg aatcaaattt ctaacgcttt gacacattca ggtatttcaa
      421 ctacaaaaat ttatgtgaat acacctgatt tagttgataa agctgtgttc gaagcgtttc
      481 agcgaggatt acaaaaatct aattaatgtg ttctagaatg tgttctaaat ataaaaaaaa
      541 gacactttcc aaataattgg aaagcgtctt gaacgttgat atataaagga tattaacgtt
      601 ttgagaattg agatgcttta cgagctttct taagacctgg tttcttgcaa aattatatta
      661 gcctcaactg aaagatttat ttttttgctc cctaacttca ttagtaatac catgtttcat
      721 tcgatatctt ctaaacgttc tacgattaat ccctgttagc tcttcaactt ctttatctgt
      781 agatccagtc aaaaacaaat taaatgctaa ttgaagtcgc ggatcattct cacgatattt
      841 tagcgtacct cccttgtatt tcccttttgc tttagcgagt gcaattcctt gttgttgcgt
      901 ttcgcgaata taccgacgtt cttcttcggc ctggtacttg tatagttcga taattaagtt
      961 attaagtaat cgacgtaaat tatcatcagt gatacctgtt aacgctggca aattcaaaac
     1021 ttccagtgta gctttcttca gctgaattaa attcatcgtt tctgttagct ctttattatt
     1081 tcgtccaagt cgttttaatt ctgtcacaac gacaatatca ccttcacgta tgaatttcat
     1141 catttcttga aatccttctc gattggtatc tttcccactt gctttatcac taaaaatttt
     1201 atcacactct gataataatt ctaattgtcg gtctaaattt tgatctaagc tgcttactcg
     1261 agcataccca attttcgcca taataaaact cctttaaaat tctgaaatcc ggatcatttt
     1321 taaattctgt tgcccaactc tgttgtcatt tttctagata aatggcataa attttcagcg
     1381 ctaagtgaca aaagcaacta atttttcatg atagattttt aaatatctca atctatctcc
     1441 aaaattagtt gcttttgtag ctatagttga ctatgaatat gacaaataat tagaaaaaac
     1501 tgtgccaatt aaatataaaa atgacactca tttacattca atagaacacc aagagcccaa
     1561 cagggtatac tctggtgtcc ttttaaaaaa gtaactttct gacctaatta attgagctac
     1621 taaaaaagct atgaaatgag gaataaaaaa gggatcaaaa acttttaagt ttctgaccct
     1681 tttgagttct aattatttct tccagaacga ag
//
LOCUS       DAXWLB010000262         1709 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXWLB010000262 DAXWLB010000000
VERSION     DAXWLB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.270"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            19..828
                     /locus_tag="ACGE4H_002791"
     CDS             19..828
                     /locus_tag="ACGE4H_002791"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFF8366153.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            868..1365
                     /locus_tag="ACGE4H_002792"
     CDS             868..1365
                     /locus_tag="ACGE4H_002792"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFF8366154.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          601 a          303 c          325 g          480 t
ORIGIN      
        1 agaaagtagg aagataaaat gaaactagca gcgattgatt tagacggtac cttgcttgat
       61 cgaaatggaa agataccaga agaaaatatc caagcacttc ggaaatttga tgaaaatggc
      121 ggaatcgtaa ccgtcgctac cggaaggaac agcatttctg ctaaagatat tttttccgaa
      181 ttaggaatcg gtggctattt gatttcttcc aatggttcac tgattgctga aatgaaggat
      241 ggaaaaatgg cgaaagttct cagacgttca aaaatagata gcgatacact gagaaaagcc
      301 ttcttctatg ctcaagaagc gaaaatatcc atcattgcta gccgagaaac ccaagacgat
      361 cagctgacat tcgatgaatc aaatctagtc aaagatgacc cttattatga acatttcaat
      421 ttacagaacc attcttttga tcaaatttta aatatgttgg aagatccttc tcttagctat
      481 cttaaattag cattaacaga taagagtgaa gaaaaactac agaagctaca aagagatctg
      541 caagcagatg gtatccaatc agtattctct gatccgcatt tcttagaaat cacgcctaca
      601 ggtatcacta aagctcattc acttgttttt ctggcggatt accttggaat cgaatcgagt
      661 gacatcatgg cttttggcga tcaagaaaac gacattgcta tgttgaatta cagcgggttg
      721 agtattgcga tggggaatgc acagcgacaa gtaaaagaaa ttgcaaacca agtaacactg
      781 acgaatgaag aagctggtgt tgcttatatt ttaaaaaact tctattaaaa agaataaaat
      841 gacgatatga atgtaggagg aaagaaaatg ttagttatta ttgatatgca aaatcatatt
      901 cttgatccaa acagcgaatt ttatattcca gagtcagatc aattggcaga acgtattgct
      961 caaagattag ctaaagcacg ccaaaataat gaatatattc tcttcacacg ggatatacca
     1021 gttgaactga aggacaaaga agaagaacga gaagatttgc aattgatccc tcttctttct
     1081 ccacaagaaa atgaacgagt gattaaaaag tattacttta cgattccacc tgaaacttta
     1141 gctgaaatcc aggaaacttt atttgaaagc aaagaagaac aaaaagagat cgaagtagtc
     1201 gggattgaaa caaacttgtg tgtactctct aatctgatag gcctgcagag tgcttttcct
     1261 gaagcagact tttttgttga tccaatgtta gtctccagta gaaaacacgg agaatccgct
     1321 ttagagctgt tgaaagattt caacgtctct atcgtcgaat cataaaaata tctatcttca
     1381 tttacgatat ttaacacaca ataaaaaaag cacgcagaac tgttttagtc ctacgtgctt
     1441 tttctatcaa agtagattgg cttttttatg acacagagtg aaacggcagg ctcacgacct
     1501 ctttcttaaa tggtgttcaa tcagctgacc ctcggtatta gcttaacaaa cggcaaaata
     1561 tgaggaacta tttttccgtt tgttaagcta atactcaggt caaaacgctg atttcacaac
     1621 ctcttcctta aacggtattc aaaagcttgc atctgcagta ataagaacag ccaagtaaaa
     1681 acataagaag ctgtttttac ttggctgtt
//
LOCUS       DAXWLB010000263         1679 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXWLB010000263 DAXWLB010000000
VERSION     DAXWLB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.271"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..861)
                     /locus_tag="ACGE4H_002793"
     CDS             complement(<1..861)
                     /locus_tag="ACGE4H_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFF8366155.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYI"
     gene            complement(1020..1286)
                     /locus_tag="ACGE4H_002794"
     CDS             complement(1020..1286)
                     /locus_tag="ACGE4H_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8366156.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1276..1632)
                     /locus_tag="ACGE4H_002795"
     CDS             complement(1276..1632)
                     /locus_tag="ACGE4H_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8366157.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          481 a          322 c          204 g          672 t
ORIGIN      
        1 aatatagcgt gatggatact catcaaaaga agaatcgtat tcttccttta aaattcccga
       61 tttacttaat ttttctagta catcaaatac tctatcactc aagtaactcc ttaaattgtt
      121 atcactttcc cattgaacta cttttctata agttgaatta tcagttgaaa agatagaatt
      181 ttcatccata aacgcatata ttagcagcaa tctttcatca ttgtcaaatt ctattggatc
      241 aaaaatatct aagttgtcat caaaatcttt atcagttccg gtaacttttt tgttaattac
      301 tgaaggtgtc ttgtaagaaa tagaatctaa atcaaagcgt tttgattcta aattattttg
      361 tgctttctca attaaataat tcactttttt tgtgagaaac cttctgatat tgtctttgct
      421 atatgttcca taaaaaccct tatcttctaa agagtccctg tacttcttaa cttctatata
      481 ctcttcagcc aaaacttgtg gaactggttc atctaagaaa aaaaccccta cctcgccttt
      541 gctattttta taaaagtaat caatttctgc tatagttccc gatctataat tatcaactgg
      601 agaacccaac ttatggccaa aaatcgctaa taatatatca ctattttcta caatttgttt
      661 attaataaca ctttgcccag agctattatt tatataagta ggaatcgcat tcgtttccca
      721 tcgaattggc atcaaaatta cttttgtatt atctgtattt tctatattcc atctatatat
      781 ggtttcttct acttcattcc tctttgctgc tatatcacct ggtgatgcta taaatacttt
      841 tattagattt atggttttca tttattgtac ctttctcttt atgtctcttt aaagtaattt
      901 ttgtataaaa tttattgact tttacactat aggacaactt ttatctattt agtacaaaaa
      961 ggcccactaa actaattgat tagttaggag cctattacag gtatattttc aactcgcttt
     1021 taagcaaatt ctactaccag ctttttgcct aatgccctag cgatatcatc aagtttatcg
     1081 aaagatacgt tatcccctct ctcaattcga gcaatagtag attgagttgt atgtgctttt
     1141 tgtgcaagct ccgcttgagt taggcctgct tcctcccgtg catgataaag agcaaccgca
     1201 gtttcgagtt ttcttccttc acggtcatat gcttctgcaa aatctttgtc tttttgcttt
     1261 tcttcaatca attttttaat catcatttgt gaaccactcc tctctaagtt cttttgcatg
     1321 tttaagttca cttattggtg ttttctccgt ttttttagta aatccatggg taatgacata
     1381 ccttccattg accacatgga aatagatagc tctttggatg tttgatccta ccttacttct
     1441 taattcgtac aaattagtgt ctaatttttt cacccatttc aaccgttgag ctactagcat
     1501 cccattcttt tctgtttctt ctattgtacg taaaagcttc gcactatctt tttttggtaa
     1561 tgtttccaac cattctaaaa attcatcatg accattaggt cttttaaatg tttcaaaaga
     1621 tattttttcc atacccaatt atatcatata ttgattttat attatagtat ataacataa
//
LOCUS       DAXWLB010000264         1659 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXWLB010000264 DAXWLB010000000
VERSION     DAXWLB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.272"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..248
                     /locus_tag="ACGE4H_002796"
     CDS             <1..248
                     /locus_tag="ACGE4H_002796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366158.1"
                     /translation="NNIKKKFKQQFKKILTAEIDVAISQSSTKYDPAFQKLIDKCLFI
                     IKIKEKFMSAFFIKVVMIIRLLIVFFRKYGIIVLTKR"
     gene            complement(968..1477)
                     /locus_tag="ACGE4H_002797"
     CDS             complement(968..1477)
                     /locus_tag="ACGE4H_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366159.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          558 a          278 c          248 g          575 t
ORIGIN      
        1 caaataatat aaagaaaaaa ttcaagcaac aattcaaaaa aatcttaact gctgagattg
       61 atgtagcaat atctcaatca tcaacaaagt acgatcctgc ctttcaaaag cttattgata
      121 aatgtttatt cattataaaa ataaaagaaa aatttatgtc agcttttttc atcaaagtgg
      181 taatgataat tcgtctatta attgtatttt ttaggaaata tggtataatt gttttaacga
      241 aaaggtaatt catgatacac caaaaaccac tagctggcgg gctagtggtt ttcttttacg
      301 ttctattcag attaacgggt aggcttactg tcttttttgg caataatttt gcaatcaagc
      361 catttacaga tgtaatgcgc ggatacttct gctaagattg caaaaaacat atcgaaaagg
      421 taattcataa tacaacctcc tccccttgcc ataataggcc ggggtagcag acagcataaa
      481 tattatacca taactgaaaa tttcatttta gtacatttca atatattgat gaaccagcac
      541 aataaaacat gccatgacta acagaaaaaa acttattaaa ccagatataa cttgtaaaca
      601 tccaggtata tagaaaacgt aaattttcta tatacttgga tatgctggaa tgtttaagag
      661 aataattaca tttctagaaa atacaataaa aattacagga taattagcca tatcctacct
      721 cctctagcag ttgctcaaaa gttagcacta agtaacttct tttttgttgc tggtaacaat
      781 ttatcaatac taaaatagcg tttaagtatc taataaatac taattttaaa gctcttaatt
      841 attatgaaac acgatataac aatattgcta tatcgtgcta ttgtaatatt atacatttga
      901 agacagcgta ttcttgttct aatatcagga attttttata aatatcagtc atattaagac
      961 tcaaaattta ttggtatgtt cctctgtata gagtttgacc gtctaccatt atatagcaat
     1021 tattatcatt gttgtcttgc accaatatac cgttaagggt agcactagga gtagtaacaa
     1081 tcactggaaa ctcatgagaa agctcggtca tttttcctgt tctgctagaa tcgtgttcta
     1141 ttgtcaagcc atttactata aacgtgaata ctttacttcc tattgttata gtagcttttt
     1201 gagaaacagt ttctgtagct ggagctgtgc ttctttccga atttgttagg gtttcactgg
     1261 attcattgct ataaacaatt gtagaggatt ctacggcttc ttgagtgtcc acgttggtgc
     1321 tgctagcttc atctagcttc cttttgtatt gttcgttact gattgctaag ctttcccgtt
     1381 ggtcatcaag cttattcatt tctgtaatgg tataaaataa tcctcctgct agtgctatat
     1441 taacaactaa actaaccgca aataatacct ttttcattct tccttctctt ctttctcttt
     1501 ttgtaattcc ttacttttgg ataggctaca attaactaga cagaaaaatt aaggtgtgta
     1561 gactagaaga aaacatacca ggaggaattt ttatgtctaa gagaacacga agaacttttt
     1621 cacaagaatt caagcaacaa atcgtcaatc tttacttag
//
LOCUS       DAXWLB010000265         1604 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXWLB010000265 DAXWLB010000000
VERSION     DAXWLB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.273"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            92..1558
                     /locus_tag="ACGE4H_002798"
     CDS             92..1558
                     /locus_tag="ACGE4H_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFF8366160.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          446 a          292 c          334 g          532 t
ORIGIN      
        1 agtttatatt tcttaaataa gttttttagt taaatttttc ggtaaagatg tataatattt
       61 tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt
      121 cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta
      181 ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact
      241 tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat
      301 gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt
      361 cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag
      421 tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt
      481 ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc
      541 gattttttac atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat
      601 cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga tgttctttgg
      661 attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc
      721 gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt
      781 tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt
      841 tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct
      901 gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt
      961 ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt
     1021 tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca
     1081 atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa
     1141 acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg
     1201 attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag
     1261 tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc
     1321 agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc
     1381 tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta
     1441 cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg
     1501 tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc
     1561 aatgaacata aaaacaggaa ttgcttattt gcagcagttc ctgt
//
LOCUS       DAXWLB010000266         1601 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXWLB010000266 DAXWLB010000000
VERSION     DAXWLB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.274"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            1..1601
                     /locus_tag="ACGE4H_002799"
                     /pseudo
     CDS             1..1601
                     /locus_tag="ACGE4H_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          638 a          262 c          346 g          355 t
ORIGIN      
        1 aagaaaaaca agtagaaaaa cttgaaagac aagaagagat aaaggaaatg ggctataaca
       61 agtacggcgg actggatatg acgatcgaga atattccaag aatcaaagaa gaaatcgaac
      121 gctttgaaaa aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat
      181 tggaaacatt agaacagctg aaagaaagat cggaaaaagg aaaagaaaac cttcttcctg
      241 aagtacaagc aatcattgac agcggacgag tgacgcaatg gaaaaaaatc cgaccattta
      301 ttttctgaaa ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga
      361 atctaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga
      421 agaggctgta ccaactgatt ttttgaagga gaaagaaaac gatcattttt atcgagtaga
      481 attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt
      541 cgaattgatc gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta
      601 taaattctat tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct
      661 aggggatggc gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca
      721 agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat
      781 gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat
      841 taaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcatactca
      901 ttctcctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt
      961 tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc
     1021 cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt
     1081 cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaatagaagc
     1141 tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaaactta
     1201 ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc
     1261 gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact
     1321 tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga
     1381 aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt
     1441 tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac
     1501 aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac
     1561 taaagaattg caggcagaaa tgacttctta tattgtgtgc a
//
LOCUS       DAXWLB010000267         1595 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXWLB010000267 DAXWLB010000000
VERSION     DAXWLB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.275"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..257
                     /locus_tag="ACGE4H_002800"
     CDS             <1..257
                     /locus_tag="ACGE4H_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366161.1"
                     /translation="NETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDVD
                     RNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(619..858)
                     /locus_tag="ACGE4H_002801"
     CDS             complement(619..858)
                     /locus_tag="ACGE4H_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366162.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1144..1458
                     /locus_tag="ACGE4H_002802"
     CDS             1144..1458
                     /locus_tag="ACGE4H_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366163.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          547 a          219 c          279 g          550 t
ORIGIN      
        1 ataacgaaac tatcatttta ggaaaaaaat atatttgcca acctattggc ttgaaacaaa
       61 aagtgattgg agaagtaatc agtaaattag aaaattgtgt tgtgatctgc gtagacgaat
      121 atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg aaaagtagtg gcaaaatata
      181 ctcacgttta tgagttggaa acaacagaat atctatataa ctatagcaac aaatatgaac
      241 atactttagt ttcataaaga tttaaataaa gtgccttcaa aagcgagaag accaagcttt
      301 tgaaggcact ttatttagtt ggcagtgatt gttcatgatt tttgatttat tcaaaaggtt
      361 cgcctagaga atctgtctct gtaacgaggc ctggtggaga aactcgataa aaacctatac
      421 tgccagaact gtcctatttt cggatagaca aactagtagt tttaaaaaga tcatccgatc
      481 caatcatttc aaaaaatgca aaactgatat caggatcatt tagctcaggc ttgttatcgt
      541 aaagcatttg taaaggttgc atttagttag gagtaaacgc gaaactatca tcattatttg
      601 ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa taaaggagac gtgctgacag
      661 tttctttacc ctgttttttg cagaggtaga aacttttata tcggaagttt ctaccggaga
      721 atttttctca tttgtagaaa ttgttgaatc gatactattc gaaatttgcg tatctgaagg
      781 tatgttcttt gatgcagaag tcccacaagc agtaagtaac aataaatttg ataaactgat
      841 aaacagaatt cttttcatta tgtacacact ccttatagta tcgtttagca tacacctatg
      901 ctcagtatta ggcaaaatca atgcaaaact atcaagtctt ttatagtatc tattagtctt
      961 agtaatacat atatggagag ggtgatatcc cagttcaaac cagatacatt ggttaccatc
     1021 tgtattaacg attatgcctc taaacgcttt ttttatgaaa aaatttataa gtgaaaacgt
     1081 aggtatgata ttatctagag gtaatttttt tgggtattta aatagttgct aggaggatag
     1141 cttttggatt tttacagtaa ttttatctta ataatagcaa tattattact tctaaatatt
     1201 tggttttttg ataagagtag aaatgcaggg ataggtttta gaaccaaacg aagtacgtct
     1261 tctgaaaaaa agtgggtata ttcacagact attttttacg gaggggttat ttcaatcagt
     1321 ttactttctt caacacttta ttcttttaat gttatagacg tttccatgtc aaattttata
     1381 tctattattg gaatccttat ttctgcaatt attacacaac tacttcttgt gtttgaagag
     1441 aagtcaaaaa ataattaaag caattatgcc ttttagtaca ggaattatta cttcttacaa
     1501 atgtatagcg aatgtgaagc ctgctgtttt aattgttcta gaaaaacttt tgagtgaaca
     1561 attattttat gttaggatga attaatttct ctaga
//
LOCUS       DAXWLB010000268         1578 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXWLB010000268 DAXWLB010000000
VERSION     DAXWLB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.276"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1518
                     /locus_tag="ACGE4H_002803"
     CDS             <1..1518
                     /locus_tag="ACGE4H_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366164.1"
                     /translation="KKKSNTKLDIFLFLSEHRFFITTQYLADHFHMSESNFLLYIKEL
                     EQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESVSYQILTSLFSCQ
                     TNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSFSGKEVQIQYAAL
                     DIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFP
                     ATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILARLSITKIESEET
                     SLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKLYNGFLNF
                     NQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLV
                     HLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDC
                     PLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT          568 a          256 c          245 g          509 t
ORIGIN      
        1 aagaaaaaaa gcaatacaaa attagatatt tttcttttcc tatctgaaca tcgtttcttt
       61 attactacac aatatttagc agatcatttc catatgtctg aatcaaattt tttactatac
      121 atcaaagaat tagaacaaga ctttgagcgt ctcaatctaa cagaactgca tattgataag
      181 caaaaacctt ttttaaagtt gaattttgaa ggaattgatc cggcttactg ctattatcgt
      241 ctatttggaa gatactgcaa cgaatcagtc agttatcaga tcttgacttc tttgttcagc
      301 tgtcagacaa attcgattat cagtttaagt cagcaaacaa actatagtgc ctcttatttg
      361 tatacgaaaa tgaaaaagat taatgcattt ttagcattgt acggtttgtc tatctccttt
      421 tctaataatg gcagaaagtc tttttctgga aaagaggtac aaattcaata tgcagcatta
      481 gatatttatt ggaacatctt ttcaagtact gctacagaat tttatgatga agatccgcaa
      541 gtagtggctt ttatgatgaa tacttttttt aaaaaagaag tacttgaccg actaaatagc
      601 ggtatgatag ataaattata ttttctatta aaaatatgta aagagaattt tcctgcaact
      661 tctgtgaaag acgtacaaaa agaattcaca gagtatgaat atattgactt ttttattgat
      721 ccctctgtag atattttgcg acctaatctg ccaatttgtc gcgaacaacg catcttagtg
      781 aatattttag ctcgactttc tatcacgaaa atcgaatcgg aagaaacaag cttggagcaa
      841 tacaaacttt taaaaaaagc aaacgttcct catgttcttt attcaagaga aatggtggag
      901 tccttttgta ctgtatttga tttgtttata cctgaaaaag aaaagattct ttactcattg
      961 gcattcgcac gaaataaact ttataatgga tttcttaact tcaatcaacc aaatacgccc
     1021 cttcctactt tcatgcttta tcaagaacat agcgtgttga aagaagtcca aaccgccatt
     1081 gaacaatttt atatggattt cagagaacaa aatcaacact tgttaccagt tattttagaa
     1141 aaggaaaatg tgcaatggat ggttgaagac ctagtacatt tgtatgatcg ctacaaaaaa
     1201 cagccgcgaa ttgtgattgg tgtgaactat acgcgtgatt tttatatcag taaagatctg
     1261 atcgttaaga tagaacaaat tttttcaaat gaatcgatca aaatacaaaa tgattttatg
     1321 gagaactgca acatcgttat cagcgactgc cctttgaaac aattgccttc tcatatcaaa
     1381 aaaatctata ttttaaaagg aatcatcaca ccagaagact ggaaaaaagt gattatgagg
     1441 atctcagaat atatctttga attaaaagaa gaagcactca tggatcaatc gaaaaaagaa
     1501 aatcaagtta cttcctagac caaataagat acataaatcc gaatgcttat taacaacctc
     1561 cggatttatg tatcaaga
//
LOCUS       DAXWLB010000269         1571 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXWLB010000269 DAXWLB010000000
VERSION     DAXWLB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.277"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(173..1030)
                     /locus_tag="ACGE4H_002804"
     CDS             complement(173..1030)
                     /locus_tag="ACGE4H_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFF8366165.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1316..1570)
                     /locus_tag="ACGE4H_002805"
     CDS             complement(1316..1570)
                     /locus_tag="ACGE4H_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8366166.1"
                     /translation="MKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSS
                     GFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          460 a          316 c          217 g          578 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccaatc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
     1261 gaatcagatt ttcgataaaa aagaagccgt ccttcttaag aacgacaaca aatcactaga
     1321 ttaatttttt caactgttca taaaagtttt catgcgttcc acataacaat actggaataa
     1381 ccttgttttc tataatctcg taagctactc tatatgttgt gcccgcatat ttgaacccac
     1441 tagaccaaat acctgataaa tccccagttt tttgttcacc cttatatgga tcaatcgcaa
     1501 tttcatcata aattaaattc aaaaattttt ctttcaatgg cttctctttt agctttttta
     1561 aatacttaat g
//
LOCUS       DAXWLB010000270         1562 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXWLB010000270 DAXWLB010000000
VERSION     DAXWLB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.278"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            132..506
                     /locus_tag="ACGE4H_002806"
     CDS             132..506
                     /locus_tag="ACGE4H_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFF8366167.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACGE4H_002807"
     CDS             complement(620..1249)
                     /locus_tag="ACGE4H_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFF8366168.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="ACGE4H_002808"
     tRNA            complement(1383..1455)
                     /locus_tag="ACGE4H_002808"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          535 a          232 c          271 g          524 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 at
//
LOCUS       DAXWLB010000271         1561 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXWLB010000271 DAXWLB010000000
VERSION     DAXWLB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.279"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            39..410
                     /locus_tag="ACGE4H_002809"
     CDS             39..410
                     /locus_tag="ACGE4H_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366169.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            495..695
                     /locus_tag="ACGE4H_002810"
     CDS             495..695
                     /locus_tag="ACGE4H_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366170.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            886..>1561
                     /locus_tag="ACGE4H_002811"
     CDS             886..>1561
                     /locus_tag="ACGE4H_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366171.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQS"
BASE COUNT          629 a          155 c          286 g          491 t
ORIGIN      
        1 taaataggac acaaaatttg gaaagtaggt aagtcactat gagagaacta actttatctt
       61 ctaaggaatt agaaaagaaa actgctttat taaacgaaat atttagtgct gattgtataa
      121 tccaaagttt aaaagaaagt tcattttcaa aactaacaga tcaggaaaaa atttcttata
      181 tgctaagttt agataatcta ggaactaaat atgatgaatc agcaagtagc gataagcttt
      241 tgattgaatt agtatcaagt cttagtagtt ttggaaaaat gaaaagattt aatgtgatat
      301 tagaaatttt tgcttggtta atcccttcat ttatttggtt tttttatatt ttcatagttg
      361 aatctgagtt aatagccaag gtctctcttg gactgttaat tccgatataa ataggttcag
      421 caatttactt taataacttg tatacaccta ttcaaataaa aaagcagtta ttacatgaag
      481 gattaagtgg taatatgtta aaaagaattg catgggataa agataataaa acagatattg
      541 agcttaaaga atataaatac aattatttct cgagatgtat agatagaaag ttactgttta
      601 aaaaagaagg attagaattt agttttgatg aagatgaatt tgaaaaatta gtgatggact
      661 ggcgtaagaa tttactggat acggtacaat tctaaaagca attgaggtcg aaattccgat
      721 acttgaatca tgatctatat tatcgtgtta tgagtgaaat ttctaataaa gataagatat
      781 ttcatttgga cagtcatgtg aatatgagct taacaattaa acgtatttat gatcttttat
      841 atgtttataa acattaatga ggtgctaaaa ttgacaacaa taactatgac aaaaaaaata
      901 gaagtatatg ttgatggatc acaagaagaa agggattcct tttttgattt tttttggaaa
      961 aatagtagag aatatgtaaa agcgttgaat ctggtatata aacagctaat gtttgaagag
     1021 ttgataaaag atgcaattaa agaaatggat caaggttatg ctgagagaga agaaagtatt
     1081 caaaatcgga ttgaaaagtt agcatatact gatccaatag atcaaaaaaa acttgataaa
     1141 gcaaaagctg aattatctaa attgagaaat gaaaaaaata aagaagcgag agaaatatta
     1201 acgcaagcga tagggttgaa gagtcaaacg agaacacgag atgtagcaaa aaaattagac
     1261 tttgactttg gagattttgt tgattccgca aatatgaaag ctagtaaaga ttttggcaat
     1321 gattttatcg gaattgtgac cggacaaaga acgcctagaa tatataaaaa tagaaacaaa
     1381 tatggattat taccttttag aggtcgagat attaaaatcc ataaagatga taagttttat
     1441 atttatttac ctaaaggctt taggtttaaa gtgaatttag gtagcaagcc aagaaaagca
     1501 agagatgttg cttcagtttt acaacacatt atggatggcg attacaaatt aaatcaaagt
     1561 a
//
LOCUS       DAXWLB010000272         1517 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXWLB010000272 DAXWLB010000000
VERSION     DAXWLB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.280"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..486
                     /locus_tag="ACGE4H_002812"
     CDS             <1..486
                     /locus_tag="ACGE4H_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFF8366172.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(693..1217)
                     /locus_tag="ACGE4H_002813"
                     /pseudo
     CDS             complement(693..1217)
                     /locus_tag="ACGE4H_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1321..>1517
                     /locus_tag="ACGE4H_002814"
     CDS             1321..>1517
                     /locus_tag="ACGE4H_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366173.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTV"
BASE COUNT          489 a          286 c          294 g          448 t
ORIGIN      
        1 ttaaaggaag cgatcgatca aatcaagacg gctgtcttgt atcctcataa cggattaccg
       61 attttattga tgggtgtaag cggttcagga aaaacctttt tggcagaaaa aatttatgaa
      121 tacgccaagg aacaacaggt gatagaatca aatgctccat ttatcgccta taattgtgct
      181 caatacttta atttctcgat taacaagatc gtagaaaatt taacgatcct tgatgcaatg
      241 aaggttttga gattggtaca aaaggccgtc aaagaactcg aaaaactaat ggggatcgaa
      301 ttttcaaaca atcaattgtt cttgctttac atgcactgct gctcaatgat cgaacgtatt
      361 ttacgcaaag aaagtgtaga tgaacaagca gatatcaaag aatatattca aaaagaggga
      421 cataattatg aaatatcaaa atacccggct tacaaaacga agtttgaaag tactgtaata
      481 aagtaagatt taaataagca taaacaaggt aagaaccata ggggctgtgt caagtccaaa
      541 atcttgtgta aaaaggaaat ctcaaagtac gatgttttgc cttgttgagc ctagctacgg
      601 tccaatatgc tggtaagaag tgtcaagagc aaaaagcgaa gcgtctcttg atacttctca
      661 ccagtatatt atactattgg cggcttaatg tgctaaatct cgttacgatc aaattcaaga
      721 tattttctag gtgcagaagt ccattcgtca tccttttcca tcaataaagc accgatgata
      781 cgaataacag aatcacaatt tgggaaaatt cggacaacac gttcacgacg gcgtacttct
      841 tcatttagtc tttcaagaac attcgttgtt ctcaatcgtt gacggatatt ttctggtaaa
      901 accattactt gcatggcatc ttcgaagccc tcatccaata ttttacaggc agttgcgact
      961 ttcttgtctt ccccatattt atccataata agttgtttac gttctccagc cagctcgatg
     1021 cttgccgttt tgaagatggc tttaaagtct tctttgatat cgcttgagat tttcttggag
     1081 aatttgtcgg aaatgttgcg aaggagatgt gcttggcatc tctgctagct cgcacaaaga
     1141 aggtattctt tcacagcttt tgtgagccct atatgcgcat ccgagatgac catctttact
     1201 cctgaaagac cgcgttcttt caatccgaga gtgtaaaata ttttgtgtaa atagaaaaaa
     1261 ggaagtccct tcagtagaat agagttacca caacacattc acagaaaaga ggacttccat
     1321 atgaacgatt ttactacaga aattctaaag actctagcga ataaaggcga tttgaatgaa
     1381 ttattccgtg tccatttgga aaaagctgtc aatacgcttc tcaaaacgga gttaacggct
     1441 ttcctcgatt acgaaaagta cgatcgcatt ggttttaaca cgggtaattc tcgtaacggc
     1501 tcctatgacc gtacggt
//
LOCUS       DAXWLB010000273         1515 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXWLB010000273 DAXWLB010000000
VERSION     DAXWLB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.281"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(192..1484)
                     /locus_tag="ACGE4H_002815"
     CDS             complement(192..1484)
                     /locus_tag="ACGE4H_002815"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFF8366174.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          412 a          342 c          271 g          490 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcct
//
LOCUS       DAXWLB010000274         1507 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXWLB010000274 DAXWLB010000000
VERSION     DAXWLB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.282"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(217..>1507)
                     /gene="cls"
                     /locus_tag="ACGE4H_002816"
     CDS             complement(217..>1507)
                     /gene="cls"
                     /locus_tag="ACGE4H_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFF8366175.1"
                     /translation="FLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTG
                     QVDVKQLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRS
                     DALGEEVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPY
                     INPRINYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQ
                     NRFLMDWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLA
                     KREILIQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEY
                     GARIETYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKA
                     FFEDSKISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT          433 a          326 c          259 g          489 t
ORIGIN      
        1 taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca tatattagct
       61 tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc tcatgcgaga
      121 cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg acacgagatc
      181 tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg aaatcaatct
      241 tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt cttttgtgag
      301 atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag ccattcgttc
      361 atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat cgatatttgc
      421 tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa taaatccatt
      481 ctcatatgtt tcgatccttg ctccatactc gattagctct gcagcgaaag aatacgttgc
      541 ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt ttacgcctga
      601 aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat aaggcgtttg
      661 gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt tgatttgttc
      721 atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag tagagtgaat
      781 ggacggaaaa aatttgggag aatagcctac ttcttttgta tgctgagaat tccagtccat
      841 caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat gattgtctcg
      901 ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga acccacctgt
      961 atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat tgatccgagg
     1021 attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg cttttttcaa
     1081 attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa ggactctgac
     1141 ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac ctaatgcatc
     1201 actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga tatcttcaat
     1261 cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt tagtcgtata
     1321 gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta cttgtccagt
     1381 aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa tttccaggtt
     1441 cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa tccctcttcc
     1501 taaaaaa
//
LOCUS       DAXWLB010000275         1504 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXWLB010000275 DAXWLB010000000
VERSION     DAXWLB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.283"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            162..1463
                     /locus_tag="ACGE4H_002817"
     CDS             162..1463
                     /locus_tag="ACGE4H_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFF8366176.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
BASE COUNT          538 a          268 c          271 g          427 t
ORIGIN      
        1 aattccgaac attttatttt caattattgt taaaagaaat aactttttct gtattttttc
       61 tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga
      121 atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa
      181 ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg
      241 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
      301 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
      361 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
      421 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
      481 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
      541 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
      601 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
      661 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
      721 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
      781 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
      841 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
      901 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
      961 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
     1021 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
     1081 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
     1141 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
     1201 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
     1261 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
     1321 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
     1381 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
     1441 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
     1501 attt
//
LOCUS       DAXWLB010000276         1483 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXWLB010000276 DAXWLB010000000
VERSION     DAXWLB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.284"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..130
                     /locus_tag="ACGE4H_002818"
     CDS             <1..130
                     /locus_tag="ACGE4H_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFF8366177.1"
                     /translation="TASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVLMGITA"
     gene            270..809
                     /locus_tag="ACGE4H_002819"
     CDS             270..809
                     /locus_tag="ACGE4H_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366178.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          548 a          202 c          249 g          484 t
ORIGIN      
        1 tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg
       61 aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggaat
      121 aacagcctaa gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag
      181 aaccgagaac cttgggtttt caagtgcttt ttggttcatt aatcttaacc atagtgtttt
      241 taaagtggaa aacaagcaac aaaaaaagta tgtggagcct tgtaggaata gttcttatta
      301 tttttactgt gactgcagca acagaagcaa attattcgca taaaattcag tcgtataaag
      361 gtcgtttagt tatgaccaaa aaagatgtta cacgttatac atcctcaaga actggaggcc
      421 tatatgacta tgcaccatta gatgcattga aatataaaga tcatttaaag aaacatgaag
      481 taaaagttgg tgatcggtgg gagaatgcat ctcataaagc atataattcc aaaatagtct
      541 atacagtaga agatgccgat gagcaaaata tcacattacc tgtattcaat tattggggaa
      601 cagttgtgaa agtaaatggg aaaaagagca ctatagaaaa tgacaatggt ttagtgtcta
      661 ttaaaggtac ggggaaaaga actatagttg aggtttcatc tacgtatcca actacgatga
      721 ttctatcttt attaattagt tcaataactt ttatattctt atttacacgt tatttaataa
      781 gatgctgcgg tgaaatgaaa atagattaga tagatattat aaatatgttg attctatttt
      841 catagcaatg gagaaaatac attagcaata tataaaagaa ggtatcaaga gtcacagtga
      901 atttattccg aaaagtgaaa agagcaaata taaaatttaa tgaaaatata gaactaaata
      961 ggaaaacgta gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa
     1021 attatataaa ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata
     1081 atttttaggt ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc
     1141 aactctttcg tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac
     1201 gatactagta tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata
     1261 gcattcctaa aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac
     1321 ttgaacatga aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg
     1381 cgcaacattt attgattgca aaattatgtg acaaaaaaaa ctagtagaag aaagaataat
     1441 ggcttgataa taaagtacta cctattaaag gtagtacttt att
//
LOCUS       DAXWLB010000277         1472 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXWLB010000277 DAXWLB010000000
VERSION     DAXWLB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.285"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            5..403
                     /locus_tag="ACGE4H_002820"
     CDS             5..403
                     /locus_tag="ACGE4H_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFF8366179.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1081
                     /locus_tag="ACGE4H_002821"
                     /pseudo
     CDS             387..1081
                     /locus_tag="ACGE4H_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          557 a          213 c          296 g          406 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcagcgata acgttaggta aagagctact
      961 agatcaagta ttagagatgc cctcgattga taaagcagtg aggggcttcc aagaagattg
     1021 ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa aaggtcgctg attacttctg
     1081 aatcaatttc tccataaaat aaacgatagc cttctcgaaa actcgaggag gctatctgac
     1141 gattcaatat tcattaattt aaagtttggg tgactattac taactattac ttatttctca
     1201 tttaaacgat gtattcccta acctctaata catccttgta actctgaagt tacgccagtt
     1261 ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt tcgtgtttct
     1321 ctagtataac agaataagaa gaacaagtcc agaagggata tttttagtct tttttaaaat
     1381 aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg gcaagtgaaa
     1441 aaatagggaa gcatgaaaga atctacttaa tg
//
LOCUS       DAXWLB010000278         1421 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXWLB010000278 DAXWLB010000000
VERSION     DAXWLB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.286"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            96..1388
                     /locus_tag="ACGE4H_002822"
     CDS             96..1388
                     /locus_tag="ACGE4H_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFF8366180.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          462 a          291 c          257 g          411 t
ORIGIN      
        1 accagcatct tttttatttt ccaaaacata tagaaaagac accttgaaat cctttagaat
       61 taatttggcg aaccaaccca aaaggaggaa tcaagatgtc ttacactcac cttatcaaag
      121 aaacattaga tattctagac ttatcagtta cttttaacga aaattgtctc acaaaagaaa
      181 aatacaaagg gcaaatctgt catatttatc gtggaaactt gatttataca gcccaagaat
      241 gcatccactg taaacaccag attgcttcag atattgtgcg ttggggtaca acaaccgtcc
      301 ggttgttaat gaacgatgtt tctgaatacc gtacctacct cgaattgaaa aagcaacgct
      361 tcaaatgcaa agcttgtcag cggacctttg tcgcagatac ttctgttgcg aaaaaacatt
      421 gtttcatcag tgagaaagtc cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa
      481 tgacagagat cgccgctcag aaaaatctat cggtctcttc tgtttaccgt attatgaagc
      541 gattttatcg tccgttgaat ccttttcgga caccgttacc taaggtactt tgttttgatg
      601 aattcaaatc agttcggggt gtttcaggtg ctatgagttt catcatgatg gatggacaaa
      661 cacagcgctt attggacatt gtcgagaacc gacaactgcc ttttttaaaa cgttatttta
      721 gtcatttttc acgagaaatt cgtgaagcgg tcgaatggat cgtgattgat atgtacgctc
      781 cttatgtatc gctcgttaaa aagctgtttc caaaagcaca gctgattatt gaccggtttc
      841 acatcgtcca acatattggt cgtacctttc gcaatcatcg aatcaaagaa acgaaccaac
      901 tattaaaaag caaagaacaa aaacactatc aattaggtaa gcaattaaaa agatactgga
      961 aacttcttca aaaagatgaa cgcaagcttg attatactcg acgcctttgg cgacctggct
     1021 tcaaagctca tttaacagag accgatattg tggatcgctt actgaaaggg agccctgctt
     1081 tgcgtgtagg ctatcagtta tatcaagact ttctttacgc tgtaaaggaa cgagactacg
     1141 tatcgtttga agaactactt accaataata tcatgcttcc cgaaggctat cagacgacct
     1201 tgagaacgtt ccagaaattt ctgccacaaa tcaaaaatgc tttacaacaa agttattcaa
     1261 atggcccact tgaatgtctg aacaaccata tcaaagtgtt aaaacgaaat gcatatggct
     1321 ttcgtagctt ttataatttc aaattgcgca tcatgattcg tcatggcaac gcattgattt
     1381 tcaactaaaa aagatcaaga ggaactgatc cacttgatct c
//
LOCUS       DAXWLB010000279         1417 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXWLB010000279 DAXWLB010000000
VERSION     DAXWLB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.287"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(150..1337)
                     /locus_tag="ACGE4H_002823"
     CDS             complement(150..1337)
                     /locus_tag="ACGE4H_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFF8366181.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAXWLB010000280         1403 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXWLB010000280 DAXWLB010000000
VERSION     DAXWLB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.288"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(1..115)
                     /locus_tag="ACGE4H_002824"
                     /pseudo
     CDS             complement(1..115)
                     /locus_tag="ACGE4H_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            204..1382
                     /locus_tag="ACGE4H_002825"
     CDS             204..1382
                     /locus_tag="ACGE4H_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFF8366182.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGAKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
BASE COUNT          470 a          271 c          259 g          403 t
ORIGIN      
        1 ctttttcttt tgcataacct tttttcttat tagtactaaa ttttgtttta aaaatgtatt
       61 cattattaac taaatatgcc acactatcat aaccactacc gattatttcg agagtgtaaa
      121 atattttgtg taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca
      181 ttcacagaaa agaggacttc catatgaacg attttactac agaaattcta aagactctag
      241 cgaacaaagg cgatttgaat gaattattcc gtgtccattt ggaaaaagct gtcaatacgc
      301 ttctcaaaac ggagttaacg gctttcctcg attacgaaaa gtacgatcgt attggtttta
      361 acacgggtaa ttctcgtaac ggctcctatg accgtacggt taagaccgag tacggggaac
      421 ttcatctcca gattccgcgc gaccgcaacg gcgagttcaa gcaacagact gttcctgctt
      481 atagacggac gaatgatacc ttagaggaga ccgtcattca cctcttccga aaaggtatta
      541 ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg gcatcactac acgccccaaa
      601 ccatgtccaa catgactaaa gtgctgactg aagaagtaaa tgcatttaaa gttagaactc
      661 taaatgataa gtatgtcgct attttcatgg atgctactta tattccttta aaacggcaaa
      721 ccgtctccaa agaagcaatc tatattgcca ttggtatacg agaagacggc gctaaagaag
      781 tactaagtta tgcgattgct ccgactgaat cgacctacgt ttggaatgag ttgttacagg
      841 acatttactc cagaggggtt catgatgtct tactgtttat taccgatggc ttaaaaggca
      901 tgaaagatac gattcaccag atttatccta aagctaaata ccaacattgc tgtgttcacg
      961 tttcccgtaa tattgctcat aaagtacgtg tcaaagatcg aaaagaaatc tgtgatgatt
     1021 ttaaggctgt ttatcaagct agctcaaagg aagaggcaaa taccttttta gggagtatga
     1081 ttgagaagtg gcagaaaact tatcctaaag tgacgcagtc actgataaaa aatcaagatt
     1141 tattgacttt ttatgagttt ccgcctggta tccgccgaag catttactca actaatttaa
     1201 tcgaatcttt caataaacaa atcaagaaat acagccgcag aaaagagcag tttcaaaatg
     1261 aagaatcact agagcgtttc ctagtatcca tctttgatac atacaatcaa aaattcttaa
     1321 atagaagcca taaaggcttc caacaagtga cggatacatt agcttcaatg tttactgagt
     1381 aacccattat tttgcagaag gac
//
LOCUS       DAXWLB010000281         1400 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXWLB010000281 DAXWLB010000000
VERSION     DAXWLB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.289"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            675..>1400
                     /locus_tag="ACGE4H_002826"
     CDS             675..>1400
                     /locus_tag="ACGE4H_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFF8366183.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYAL"
BASE COUNT          518 a          200 c          223 g          459 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg
//
LOCUS       DAXWLB010000282         1377 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXWLB010000282 DAXWLB010000000
VERSION     DAXWLB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.290"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            42..1343
                     /locus_tag="ACGE4H_002827"
     CDS             42..1343
                     /locus_tag="ACGE4H_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8366184.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          449 a          275 c          254 g          399 t
ORIGIN      
        1 tagaattgaa tcaccacaac acaatcaaag gagcgttctg tatgcctgat cttcatcata
       61 tcaaactact acttggaatc aaagataaaa acattttcat cacaaatgtg gaatcgaaga
      121 gcattaagaa tatcaagagt cttgttgttt ctgcgacgtt aagtaaagaa attcgtcgct
      181 gtcctttgtg taaacagatg aatcatgaag gcatgatcgt taagaacgga aagaaaaaat
      241 cgctcattca acttaataaa tgtgcgaacc aactgactta tcttgcttta gcgaaacaac
      301 gctaccactg tcgtggctgc cacacctatt ttacggcgaa tacctacatt gtcgatagaa
      361 actgctttat cgctaaacaa gtgcgctata aaattttaga agagctaact gaaaaacaag
      421 caatgacgac aattgctaag cattgcggcg tttcttggtc gaccgtttct cggacactcg
      481 cttctctgat ccccatgaca aaagtaaaaa gaaattggtt accgcgttgt ttattaatgg
      541 atgaatttcg ttcactgaaa aatcaagtcg gtccctactc cttttcttgt atggatggcg
      601 atactggaaa attactggat attcttcctt cacggaagaa aaaggctctc gtttcttatt
      661 ttatgcaatt tgaacgacgc gcacgattga aggtaaaggt catcgtgacg gacatgaatg
      721 tttcttatgc ctctctgata aaggaatgtt ttccaaaggc aaaactcgtt gtggatcgtt
      781 ttcacattgt caaacatctc attcgtaaat ttgaggatat tcgcgtacga atcatgaaaa
      841 gctttgatcg aaacgatcca acacaagcca aatattatcg acaattaaag gcgttgtctc
      901 ggcttctgat caaacgacag gacaagcttg tttatgacaa atggaccaaa tggcgcaatt
      961 ttggttgggc gtatctcacc gaaagtgaag tggtggatcg tctgctttcg atctctgacg
     1021 aattacgaat cgcctatttt tactatcaag aaattcttca agcgtttcac gacaaagagg
     1081 cggacacttt ctttaaactt gtaagaacga tgcccaattc cgttccaaaa gaactgcatt
     1141 ctataaaaaa agcttttata aactatgaat ccggcattcg tttagccctc gaattgcctt
     1201 attcaaatgc caaaatagaa aatctgcata cccacatcaa agcattgaaa cgagtcgctt
     1261 atggctttcg aagctttcgg aaaatgaaga ccagaatttt cttactaaat aatctgatta
     1321 cctatgaatc aaaaaacatc taaagtagaa aaggaattct accttagatg ttcttat
//
LOCUS       DAXWLB010000283         1355 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXWLB010000283 DAXWLB010000000
VERSION     DAXWLB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.291"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(296..903)
                     /locus_tag="ACGE4H_002828"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACGE4H_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
BASE COUNT          457 a          238 c          226 g          434 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaat
//
LOCUS       DAXWLB010000284         1349 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXWLB010000284 DAXWLB010000000
VERSION     DAXWLB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.292"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            1..409
                     /locus_tag="ACGE4H_002829"
                     /pseudo
     CDS             1..409
                     /locus_tag="ACGE4H_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            409..1206
                     /locus_tag="ACGE4H_002830"
                     /pseudo
     CDS             409..1206
                     /locus_tag="ACGE4H_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
BASE COUNT          513 a          217 c          269 g          350 t
ORIGIN      
        1 caaaatgaca aattgatgaa tcaaattcaa attaaggggg gcaaccctgg aagtcttaaa
       61 tttaccctca atgaatggta ttgaagatga aaatttaagg cgtttgatta atagccttgt
      121 cattgaattg tacaagtatc aagcagaatc agaacgaaaa aaaattaagg aacgtcaggc
      181 acaaggaatc gaaattgcta agaaaaaagg caaattcaaa ggtcggcaac tgaaatttaa
      241 aaaaaatgat ccacgtttaa agcatgcttt cgatttgttt ttgaatggtt tatccgataa
      301 agaagttgaa gaacaaactg ggatcaatcg ccgaacgttt agaaggtatc gagcaagata
      361 taacgtgaca gtagatcaaa gaaaaaacaa tgaaaagagg gatagttaat gagtacggtt
      421 attttagctg aaaaacaagc caggcattag cctacgcaag tgctttaaaa caaagcacca
      481 aaaaagacgg ttattttgag atcaaagacc cactatttac agatgaaacg tttatcacct
      541 ttgattttgg gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa
      601 attggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
      661 ataagggaaa gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg
      721 ttgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataaagcaa
      781 atgccttttc aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg
      841 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
      901 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata
      961 cgttaaattt acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc
     1021 ccaccttata ccttattttt cagcgccagg aagctagtat aaaagtaaaa caagggtcat
     1081 ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca agaggagctt ttttgctttt
     1141 gtttcttcta aacaagctaa aataggcaat caagagggga taattgctga tgttcaaacc
     1201 aaagagatgg ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga
     1261 aaatatacca ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt
     1321 caagcaacaa atcgtcaatc tttacttag
//
LOCUS       DAXWLB010000285         1344 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXWLB010000285 DAXWLB010000000
VERSION     DAXWLB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.293"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(156..1297)
                     /locus_tag="ACGE4H_002831"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="ACGE4H_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8366185.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          331 a          269 c          283 g          461 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc ttttcttagt
     1321 agttagattc taccaaatcg tgtc
//
LOCUS       DAXWLB010000286         1338 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXWLB010000286 DAXWLB010000000
VERSION     DAXWLB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.294"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..134
                     /locus_tag="ACGE4H_002832"
     CDS             <1..134
                     /locus_tag="ACGE4H_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFF8366186.1"
                     /translation="DESQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            261..845
                     /locus_tag="ACGE4H_002833"
     CDS             261..845
                     /locus_tag="ACGE4H_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFF8366187.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            959..>1338
                     /locus_tag="ACGE4H_002834"
     CDS             959..>1338
                     /locus_tag="ACGE4H_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFF8366188.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTV"
BASE COUNT          536 a          199 c          240 g          363 t
ORIGIN      
        1 ctgatgaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt cagaataagc
       61 tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat tcttacgcaa
      121 ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca aaacattaaa
      181 aaagcccgtg ctggcacacg gactcatacc tcatttctga gatcgcaaat atattataac
      241 aagaagtgag gaatatttaa atggcaaaaa aagttatggg tcaagacgga aaaatgtata
      301 aagttagtaa acctttttac aaaaaagtat ggttttgggt attagcagta attttaatta
      361 taattattgg ttctgcttta aatggtggat cagatagtaa taaagcaagt gataatggtg
      421 gcgaaaaagt aactaaatct tcaacctctg cttcatcttc taaagaagaa aaaagcgata
      481 ccttctataa gattggtgac actgtaaaag ttggtgatgc tgaatataca ctaaatagtg
      541 tagaactaac tgatgaaaga aatcaatttg aagaaaacca acctgcacaa gtagtaaaaa
      601 ttacttatac cgtaaaaaat gatggcgatt cagatatccc tgtaggtaca gatgtggaag
      661 tatatggacc agatgacaaa aaatcagaga catatgccaa cgaaaataca atgggatctg
      721 ttgctccagg aaaacaaatg gatgtaactg ctcatttcac gttaaaccaa aagggagaaa
      781 tagaaatcca cttctctcct ttagtatcat ttgaaaaagc agctattttt aaagcaactg
      841 tataataaat aaaaaacacg cccaccgtcc aaagcaagcg tgttctaaga aaaacaaacc
      901 tatacagtag gcttctttat agttcctatt gtatcagaga aagagagctg attcaattat
      961 ggcaagatta gtcaaacgtg gaaatagttg gcaatacgaa atttcataca aaaaagatga
     1021 cggaaaatac acgaagataa gaaaatcagg atttaagaca aaaggagaag caaaagatgc
     1081 cgccaacgaa ttagaatata acttgaacaa aggccttaaa ggggatcgca aaaatctatt
     1141 attatcagat tactttgagg attggatgca actttataaa gaaggaacag tatctcctat
     1201 cacttataga aaatacgaag atacgttaat gaacataaag aaatatatgc cagcggtatt
     1261 gatttctgat ttagatagag tgggatatca acgcttttta aataagtatg ctaaagacca
     1321 tgtaaaatct accgttat
//
LOCUS       DAXWLB010000287         1330 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXWLB010000287 DAXWLB010000000
VERSION     DAXWLB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.295"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(18..347)
                     /locus_tag="ACGE4H_002835"
     CDS             complement(18..347)
                     /locus_tag="ACGE4H_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8366189.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(424..696)
                     /locus_tag="ACGE4H_002836"
     CDS             complement(424..696)
                     /locus_tag="ACGE4H_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFF8366190.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(696..959)
                     /locus_tag="ACGE4H_002837"
     CDS             complement(696..959)
                     /locus_tag="ACGE4H_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFF8366191.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1193..>1330
                     /locus_tag="ACGE4H_002838"
     CDS             1193..>1330
                     /locus_tag="ACGE4H_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8366192.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          424 a          256 c          172 g          478 t
ORIGIN      
        1 gttttgaagg aacgatttca taagccaatt tctgttgcca actcctcttt agtcattact
       61 ttagaattag taagtgtatc ttcagcaata tctctaaagg aagaatgcat tttgtttttt
      121 ctattcttaa attctacaag aatctcatct ttatcgtagc cttctgcaat tatatcagac
      181 aaaatatctt cgctaaaatc ataaaattct ttttgtggtt caacaaattc ataaagaata
      241 ccattctcta ccaactttgc ttcaacttcg actccattag gaacattaaa atctttaggt
      301 actgtcaaca tgattgaatt gccttgtttt cttgtttttg taatcattta aatccctcat
      361 ttctaacagt atgtaattac ttagtaaata cattatacca cgagataatg tatttacaag
      421 taattactgt gactcgtaat gcgtccttgc tgaataaatt tcaaccacgt gttcatcttc
      481 atctacctta taaacaaccc ggtgttgtct attcaatctt ctagaatacc taccttcatg
      541 tgtaggtttt aacttctcaa atgattgggt agggagatag ggatcttcct ttaaagtctg
      601 aataaccttc tcaaattgag gtttcaaatt agactgtttg attttcttta aatcagcttt
      661 agccgagttt ttaatcatca cagaatagtt attcattata agttatccca atcaatttca
      721 tcaacattag taaatccact atcatctttt tctcgcttac gaacaacacc caaagtgcct
      781 atttgctcaa gtaatagcgt ttctttgatt gctttccaat catccaaacc aataagcacc
      841 gcattatttt cactgcgctc actaatgatt tcaatttccg tatgattctc atttacatct
      901 ttcaacaact tataaaaatt tttccgtgct gagctaggat ttaagactgt ttttgccata
      961 tctactcccc ctctaattta tatctccatt ctaacgtact taattaagta cgtaaattcg
     1021 attgttttgt ttaaattgga gtaccaaaac agcattgtaa agtgttatcc gtttccttta
     1081 tacccgtggt gctagttaat tttttcctag aggaaaatgt ctaaacatgt tggtaaatca
     1141 acaaaaaaac gcatagagta gtctcaaaac tcccaagaaa tgaggactac atatgcgttc
     1201 atcaaaatat actgaacatt atacagatac ttttataaca ttcaaggaaa ttatgagaac
     1261 tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa gaaatactga
     1321 tcaactgaag
//
LOCUS       DAXWLB010000288         1324 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXWLB010000288 DAXWLB010000000
VERSION     DAXWLB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.296"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            278..820
                     /locus_tag="ACGE4H_002839"
     CDS             278..820
                     /locus_tag="ACGE4H_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366193.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACGE4H_002840"
     CDS             976..>1324
                     /locus_tag="ACGE4H_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFF8366194.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAXWLB010000289         1298 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.297, whole genome shotgun sequence.
ACCESSION   DAXWLB010000289 DAXWLB010000000
VERSION     DAXWLB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.297"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(159..>1298)
                     /locus_tag="ACGE4H_002841"
     CDS             complement(159..>1298)
                     /locus_tag="ACGE4H_002841"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFF8366195.1"
                     /translation="SHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSA
                     KDILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYH
                     AEHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYEC
                     DLACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIH
                     DIDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDL
                     AQMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIED
                     VCFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT          376 a          283 c          203 g          436 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgact
//
LOCUS       DAXWLB010000290         1293 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.298, whole genome shotgun sequence.
ACCESSION   DAXWLB010000290 DAXWLB010000000
VERSION     DAXWLB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.298"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            114..587
                     /locus_tag="ACGE4H_002842"
     CDS             114..587
                     /locus_tag="ACGE4H_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFF8366196.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            596..823
                     /locus_tag="ACGE4H_002843"
     CDS             596..823
                     /locus_tag="ACGE4H_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366197.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          410 a          202 c          211 g          470 t
ORIGIN      
        1 actctatcat tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt
       61 tacaataatt attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat
      121 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
      181 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      241 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      301 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
      361 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
      421 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
      481 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
      541 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
      601 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
      661 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
      721 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
      781 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
      841 agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct
      901 attattcaga ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg
      961 actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt
     1021 ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac
     1081 tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac
     1141 cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt
     1201 ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa
     1261 catttactat gtagttttga taaaatgatt tga
//
LOCUS       DAXWLB010000291         1275 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXWLB010000291 DAXWLB010000000
VERSION     DAXWLB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.299"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(31..1203)
                     /locus_tag="ACGE4H_002844"
     CDS             complement(31..1203)
                     /locus_tag="ACGE4H_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFF8366198.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          420 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc tttta
//
LOCUS       DAXWLB010000292         1266 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXWLB010000292 DAXWLB010000000
VERSION     DAXWLB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.300"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            34..>1266
                     /locus_tag="ACGE4H_002845"
     CDS             34..>1266
                     /locus_tag="ACGE4H_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFF8366199.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFR"
BASE COUNT          389 a          253 c          250 g          374 t
ORIGIN      
        1 taatttggct accaattcat aggaggattc aagatgtctc atactcagct tatcaaagat
       61 accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac taaagaaaaa
      121 tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc agaagagtgt
      181 tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac aaccgttcgg
      241 ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa acaacgattt
      301 aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga aaaacactgt
      361 ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa tacttccatg
      421 acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt catgaagcga
      481 ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg ctttgatgaa
      541 ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc
      601 catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg ctatttttct
      661 cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat gtatgcgcct
      721 tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga tcgtttccac
      781 atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac cacggtacga
      841 cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg ctattggaaa
      901 ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg tgccagtttt
      961 aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg tcccaactta
     1021 cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca
     1081 ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta tacaaccact
     1141 ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca
     1201 aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc
     1261 tttcgc
//
LOCUS       DAXWLB010000293         1265 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXWLB010000293 DAXWLB010000000
VERSION     DAXWLB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.301"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..358
                     /gene="amaP"
                     /locus_tag="ACGE4H_002846"
     CDS             <1..358
                     /gene="amaP"
                     /locus_tag="ACGE4H_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFF8366200.1"
                     /translation="LIIVLFYPKANNYIEKKTASGKIAIQKKAIENFVLIAAKEEPFI
                     VDPTVKSVIKKKKIKIYIRGKIRKAFQTQERQTAFIRKVNTNLNELFGAEQTILTEII
                     FKDYHEKNTNTKSKVL"
     gene            368..550
                     /locus_tag="ACGE4H_002847"
     CDS             368..550
                     /locus_tag="ACGE4H_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFF8366201.1"
                     /translation="MAEFISKYKYPFIFGCLGLLLGILIFTLGFIKTVILTVLTICGF
                     YLGNYLEIVGFFDKQN"
     gene            606..1184
                     /locus_tag="ACGE4H_002848"
     CDS             606..1184
                     /locus_tag="ACGE4H_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFF8366202.1"
                     /translation="MENKTNNTKTEIKKNDVAKTSEIIKGELVFEDKVIQKIIGIALD
                     EIDGLLTIDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKEVISKEIKKMTGLDVIEVNVDVVDVKTKEQHENDSVTLQDHLSDAASTTGEFASK
                     QFEKSKEALGVASEKVSDGVENVKEASEPRVE"
BASE COUNT          523 a          146 c          219 g          377 t
ORIGIN      
        1 tctaattatc gtgttattct atcctaaagc gaataattat atagaaaaaa agacagctag
       61 tggaaaaata gcaatccaaa aaaaagcaat tgaaaatttt gtattaattg ctgctaaaga
      121 agaacctttt attgttgatc caacagtaaa atctgtaatt aagaaaaaga aaatcaaaat
      181 ctacatacga ggtaaaatac gtaaagcttt tcaaacgcaa gagcgccaaa cagcatttat
      241 aagaaaagtg aatactaatt tgaatgaact atttggtgca gaacaaacaa tattaaccga
      301 aataattttc aaagattatc atgaaaaaaa tacaaatact aaatcaaaag ttttatagga
      361 gaaagcgatg gcagaattca tatcaaaata taaataccct ttcatttttg gatgtttggg
      421 actattgtta ggaatattga tttttacgtt aggatttatt aaaacagtaa tattaacagt
      481 gctgactatt tgtggttttt atttaggtaa ctatttagaa attgttggtt tttttgacaa
      541 gcaaaattag ttaataaccg tttttacggt aagaaataaa tttctaattt ttaggaggaa
      601 aaataatgga gaacaaaaca aataatacaa aaacagaaat caaaaagaat gacgtggcaa
      661 aaacttccga gataattaaa ggagaactag tttttgaaga taaagtaatt caaaaaataa
      721 ttggtattgc attagatgag attgatggac ttttaacgat tgatgggggc ttcttctcaa
      781 atatagctgg aaaattagta aatacagata acacaacttc tggagttgat gttgaagtag
      841 gaaaaaaaca agtcgcagta gatctttcaa tagtggctga atatggcaaa gatgtaacta
      901 caatttatga taaaataaag gaagttatct caaaagaaat taagaaaatg actggcttag
      961 atgtaattga ggttaatgta gacgtcgtag atgtaaaaac gaaagagcaa catgaaaacg
     1021 attcagttac actacaagat catctttccg atgcagcttc tactactgga gaatttgctt
     1081 caaaacaatt tgaaaaatcg aaagaagctt taggagttgc aagtgaaaag gtaagtgacg
     1141 gtgtagagaa cgtaaaagaa gcatctgaac ctcgcgtaga ataacaaatc ttttttattt
     1201 aatgtacaat ctataaaatt aaaagactgt atctattgtt cttctatata tagtgttaat
     1261 ataga
//
LOCUS       DAXWLB010000294         1246 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXWLB010000294 DAXWLB010000000
VERSION     DAXWLB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.302"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            18..>1246
                     /locus_tag="ACGE4H_002849"
     CDS             18..>1246
                     /locus_tag="ACGE4H_002849"
                     /inference="COORDINATES: protein motif:HMM:TIGR02331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFF8366203.1"
                     /translation="MDNRSDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVN
                     DVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKIPVEVVDNRSDAEKYTPEVEKEEVN
                     YGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDTTQPGEYEGTIEVTYPDGTKDTVKV
                     PVEVVDNRNDAEKYTPEVEKEEVNYGGTIDLTDNVTNLEELPEGTKVNDVTPEGVIDT
                     TQPGEYEGTIEVTFPDGSSTTVKVPVTVKDKVDWVEINPIPTIDPEQIVPEEVHKGEE
                     ADLTDNILNLPEGSKVEVVTPVDTTQVGEHTGEVKVTFPDGSSVVVKIPIIVKNMDDM
                     ITNNQMTRVEKNSVVLDVINKSKSLSSSKIFGKSDNMYVETSNKELPKTGEENTTILS
                     ILGMLSISFCVLLFRLF"
BASE COUNT          489 a          188 c          292 g          277 t
ORIGIN      
        1 aaattccagt ggaggtagtg gacaatcgaa gtgatgctga aaaatatact ccagaagtag
       61 aaaaagaaga agtcaactat ggaggaacga ttgatctgac ggataatgta actaatctgg
      121 aagagttgcc agaaggcaca aaagtgaacg atgttacacc agaaggtgtc attgatacga
      181 ctcaaccagg tgagtatgaa ggaacgatag aagttacgta cccagatgga acaaaagaca
      241 cagtaaaaat tccagtggag gtagtggaca atcgaagtga tgctgaaaaa tatactccag
      301 aagtagaaaa agaagaagtc aactatggag gaacgattga tctgacggat aatgtaacta
      361 acttggaaga gttgccggaa ggcacgaaag tgaacgatgt tacaccagaa ggtgtcattg
      421 atacaaccca gccaggtgag tatgaaggaa cgatagaagt tacgtatcca gatggaacaa
      481 aagatacagt gaaagttcca gtggaggtag tggacaatcg aaatgatgct gaaaaatata
      541 ctccagaagt agaaaaagaa gaagtcaact atggaggaac gattgatctg acagataatg
      601 taactaatct ggaagagttg ccggaaggca cgaaagtgaa cgatgttaca ccagaaggtg
      661 tcattgatac aacccagcca ggtgagtatg aaggaacgat agaagttacc ttcccagatg
      721 gatcaagtac aacggttaag gtgccagtaa cagtcaaaga taaagtagat tgggtagaaa
      781 tcaatccaat tccaacaatc gatccagaac aaatcgtgcc ggaagaagtt cataaaggag
      841 aagaagctga cctaacagat aacattctca accttccaga aggaagtaaa gtggaagtcg
      901 tcacaccagt ggatactaca caagtaggcg aacacacagg cgaagtgaaa gttaccttcc
      961 cagatggttc aagtgtggta gtaaaaatcc caataattgt gaaaaatatg gatgatatga
     1021 taactaataa tcaaatgacg agagtagaaa aaaattctgt agtactcgac gttataaata
     1081 aaagtaaaag cttatcttca tcgaagattt ttgggaagtc agataatatg tatgtagaga
     1141 caagcaataa agaacttcct aaaacaggag aagaaaatac tacaatttta tctattttag
     1201 ggatgttatc aattagtttc tgtgttcttt tatttagatt attcaa
//
LOCUS       DAXWLB010000295         1242 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXWLB010000295 DAXWLB010000000
VERSION     DAXWLB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.303"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            171..785
                     /locus_tag="ACGE4H_002850"
                     /pseudo
     CDS             171..785
                     /locus_tag="ACGE4H_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          454 a          199 c          192 g          397 t
ORIGIN      
        1 tcaccccatt ctttttcttc tattataaac tatttaactt ttagaattca aaaaccgttt
       61 gtctatttag agtttaccct aaatagacaa aaatataaca ttgaaaaatt tagagttcta
      121 acgtatgatt agggtgtatt ataaacgtta tagaaaagta gggaataaat ttgaaaattg
      181 cttatattag agtttcatcc atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa
      241 agtttggcgc tgaaaaaatt tttatcgaaa aacaatctgg agcgacgatt agtcatcggc
      301 cgattttcca ggaagcttta gattttgtca gagagcaaga tatttttatt gtagaagcca
      361 ttgatcgact tggacgcaat tatgacgaga ttatcgattc agtgaattat ctaaagaaga
      421 agaatgtgcg acttattatc accagtttac cgatcatggc tgaagcaatt ggaaatcctc
      481 tcctagataa attcatcaaa gacttaatca tttaaatctc agccatgatt gctgaacaag
      541 aacgtactga atcgaaacga cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg
      601 tctataaagg tcgtcctaag ttatacagtg cagacaccaa agatcctcaa cgtcgccttg
      661 tctataggag tatcgttcag gatcttgaga atggggttgc tatctcaaag attgcaacag
      721 actacaatgt gactaggcaa acaatttacc gaataaaaaa agaaatcgac caactaattg
      781 tatgatttct tttttgaatc acgtaattat taattagctt tgtcatcaat acagacaatc
      841 attttagatt catctagcta agttgattac atttatgtta aaaagaattt agttaacata
      901 aatataattg atgcataaat cgtccttatt taaaggcatt taacattgac aacttagtat
      961 aatgtatctt atgtaaacta ataagatttg aattttattt tatacacact cgataataga
     1021 tcaattttat ggtcccccgc atttacgatt gcacaagaga aacataaagt cgacctatta
     1081 gcatttcttt tcaattcaaa cattaaaaaa gtttatacct ctgctactaa aaaatgaaat
     1141 tatctattta aaatttaaaa cggtaaatct gattcatcca aattggaaaa tggatcagcc
     1201 tgtattggag ctttgtattt ctccataata acagatatct tt
//
LOCUS       DAXWLB010000296         1222 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXWLB010000296 DAXWLB010000000
VERSION     DAXWLB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.304"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(1..289)
                     /locus_tag="ACGE4H_002851"
                     /pseudo
     CDS             complement(1..289)
                     /locus_tag="ACGE4H_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(611..832)
                     /locus_tag="ACGE4H_002852"
     CDS             complement(611..832)
                     /locus_tag="ACGE4H_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366204.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(930..>1222)
                     /locus_tag="ACGE4H_002853"
     CDS             complement(930..>1222)
                     /locus_tag="ACGE4H_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366205.1"
                     /translation="DKYYRLLLLTAEAGWMDFPEVVKEVQEIGATISDSRSSPMNYQD
                     AHGIKIIDQERNVRKYTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          368 a          236 c          163 g          455 t
ORIGIN      
        1 cgtccgcatc aattgcacga taaagataat gccaacgtcc cttaattttg atataggttt
       61 cgtccatttt ccatgaatag aaggattgtc tatttttctt cttccaaaga taatagagga
      121 ctttgctgta ctcttatacc caacgataaa tcgtagtatg acaaacattt attccacgat
      181 catataacaa ttcctgaact tcacgatagc ttagattgta acgcaggtag taaccaacag
      241 cgacaataat gacgtctttt ttgaattgtt tgcctttaaa atgattcatt actctgtcct
      301 ctctgtcttt tttctcaatt ttacactaaa atagattttt tggaaaactt tgcaacagaa
      361 ccgtttttac tatatacttc ttcccagaac ttgtccgtct ccttcaaatt atctctacct
      421 ctcgagttca ttgggtggtt tgcccattta ttaaacatgg aatcaccttt ctcatactca
      481 tatatttatg tcctatttca attatattta tatatgctta aataaatttg tatcctgaaa
      541 tttattgtaa tatgaagctg tattttacat atagtaacca aagagttaat aaaataaaaa
      601 gagcgaacca ttatagatca gctctttttt cattgctgtt tttaatctta tctagaatca
      661 cttcaaatgt ttcaaaggta tttacatcta actgacataa ccaaagcaag tacttctttt
      721 ctttgtaagt ccaatcaata ttacggtaag aggacattag attatggtac cttgctcttt
      781 gttcaactga agccaattcc cagcgttttt caaatatttt ttcttcttcc attattgatc
      841 cctcgcaaga cgctacctca aacttaaata atgggcagcg aaagtctaat tccttagatc
      901 taagtatagc aaacaaatat gaaaaaaact tagaaggtag taaaagttct tccgtctttt
      961 gctttgcttc tattcttaat gtgtttcgtt atcggtgccc tacaaagatt ttcattaatt
     1021 acacactcat ttattgttgt gtattttctg acatttctct cttgatcaat tattttaatt
     1081 ccatgggcat cctgatagtt cataggagag gagcggctat cagaaatagt tgcacctatc
     1141 tcttgaactt cttttacaac ttctggaaaa tccatccatc ctgcttcagc tgtcaaaagt
     1201 aataatctat aatatttatc tt
//
LOCUS       DAXWLB010000297         1206 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.305, whole genome shotgun sequence.
ACCESSION   DAXWLB010000297 DAXWLB010000000
VERSION     DAXWLB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.305"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(363..524)
                     /locus_tag="ACGE4H_002854"
     CDS             complement(363..524)
                     /locus_tag="ACGE4H_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366206.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            complement(517..1191)
                     /locus_tag="ACGE4H_002855"
                     /pseudo
     CDS             complement(517..1191)
                     /locus_tag="ACGE4H_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
BASE COUNT          375 a          212 c          170 g          449 t
ORIGIN      
        1 tgtgaatttt tactattctt accttagcat attctttata ggtatatcag agctaatcaa
       61 actaaaaaat acccgaagtc ttcttatgta aaaacaccgg ttattactta aatatgactt
      121 aggtaaaaca atgagtgatc caattataaa attttatggc gaataattta gtaagcgcta
      181 catattgaat gatttagaaa ttttgaaaaa gtagctagtt ccttttatac cagaaaagta
      241 aacctaataa aaaacacaac taatttttag ctgtgttttt taagttacaa taattatcta
      301 tttaatagtc ttaattcatt aaaataaaaa actcaacttt acaaggatat ggtttaaaaa
      361 atttatttat ccaacccaac tagttttcgc tgcttacgat ttgcttcatc tgctacgtaa
      421 ttcattactg ttttaaattc tttcgctgag gcgttttctg ataaatcagc gatactttta
      481 ttatacttct tcacgatttg ttgtacttgt tctttctcag tcatgtaatt cctcctccaa
      541 ctcttttgaa gtatattgta ttggtagtac taattgaata ttatctgctg cataatgcac
      601 ttcagaaatg tcgttttgat aatccttaat cattatgtga aggactactt caggatgtct
      661 atttttgact ttttcaatat tctctctgga cttaaaaaac gcattgataa acatttcttt
      721 tggcacatga cgtccttcag ctagttcacg ttttcgagtg aaatcccagg caactttggg
      781 atcttggtaa acatagaaaa tcgtaacgcg aaagttattt ttaagggctc gaataatatt
      841 tttctctgca ctaaggatag caaaagtatc ctctagtatg aaagaatagc catgttccag
      901 tacatatttt aatgattgtt caactaacca agaagaagct ttctgaaaaa cacttgagtt
      961 ctttccattg tattctggaa attgtgctct aaaatagtcc gcatcaatca caacatggtt
     1021 gtcataattt taagctaaac ctagtgcaac ctctgtttta cctgctcctg ggcttccagc
     1081 catgaatacc gcatcttttt catcatcagg gatttttccc ttgatgattg tttcaataaa
     1141 tttatcttta ttgaccctag cgtaatctaa atacttgtct ttaggttcca agattgcacc
     1201 ttcttt
//
LOCUS       DAXWLB010000298         1198 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXWLB010000298 DAXWLB010000000
VERSION     DAXWLB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.306"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            82..>1198
                     /locus_tag="ACGE4H_002856"
     CDS             82..>1198
                     /locus_tag="ACGE4H_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFF8366207.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQ"
BASE COUNT          398 a          231 c          232 g          337 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaa
//
LOCUS       DAXWLB010000299         1168 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXWLB010000299 DAXWLB010000000
VERSION     DAXWLB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.307"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1081
                     /gene="mobL"
                     /locus_tag="ACGE4H_002857"
     CDS             <1..1081
                     /gene="mobL"
                     /locus_tag="ACGE4H_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFF8366208.1"
                     /translation="ISFDNDFLVREGYYNPETNELNENILYEATKSMMGKMQEKEELV
                     DPFWFATIHRNTEHIHIHVTAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSK
                     ILDRKNEFEKISKLRKEVPLELKQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPS
                     TTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYY
                     LNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTK
                     YAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKN
                     DYEVAQRMIEQEKMRQAHEFEIGMN"
BASE COUNT          533 a          127 c          204 g          304 t
ORIGIN      
        1 aatttcattc gataatgatt ttttagtaag ggaaggatat tataatccag aaacaaatga
       61 attaaatgaa aatattctat atgaagcaac aaaatctatg atgggaaaaa tgcaagaaaa
      121 ggaagaacta gttgatcctt tttggtttgc aacgattcac agaaatactg aacatatcca
      181 tattcatgta acagctatgg agcgtaaaaa tactcgtgaa ataatggaat atgatggtgt
      241 tttacaagct aggggtaaga gaaaacagtc tacgttagac gatatgattt ttaaattcgg
      301 tagcaaaata ttagatagaa agaacgagtt tgaaaaaatt tctaagctga gaaaagaagt
      361 tcctttagaa cttaaacagt ctgtaaaaga ttctttgatt caattatatg tagaaaataa
      421 ttcaagatat gacaaagaat tagtaaagta cctaaaggaa ttaaaaaaag aaatacctag
      481 tacaacacgt ggctacaatg agcttcctga agaaacaaaa gaaaagattg atgaagtcac
      541 aaattatatg acaaaggata atccaaaaag aaaagaatat gatcgtatga ctaaagaaat
      601 tgatgagcta tatcaaacaa cctacggtaa acgctacaat ccacaagaat attatttaaa
      661 tagaaaaaaa gattttaatg ctcgaatggg taatgctgta gtaggacaaa tcaaactttt
      721 aaagcaatct gaagaaaaaa atttgaaaag taatgaactt aaaaatctat tagacggaaa
      781 aaaattaaac gttaagtttg atagagaaaa accagaattt aaaacgaaat atgctaaaca
      841 aagttatgat gatttccaaa aaagattaga agaacgacaa ttagcacgtg aaaaaaatga
      901 agtccacttc aagaagattt ctgaaaaatt taaagataaa aagcttatta gaaaaattga
      961 tagagtaatc aatgatgatt taaatatgta tcgtgcgaaa aatgattatg aagtagcgca
     1021 acgaatgata gaacaagaga aaatgagaca agcacatgaa tttgaaatag gaatgaatta
     1081 gtagaaacgg agaatagaaa ttatgaaaaa cttcgatata gtgtgcagta atacaaaaaa
     1141 tatatattta cgtgagctac ttaacagt
//
LOCUS       DAXWLB010000300         1165 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXWLB010000300 DAXWLB010000000
VERSION     DAXWLB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.308"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(82..171)
                     /locus_tag="ACGE4H_002858"
     tRNA            complement(82..171)
                     /locus_tag="ACGE4H_002858"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(195..268)
                     /locus_tag="ACGE4H_002859"
     tRNA            complement(195..268)
                     /locus_tag="ACGE4H_002859"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Ile,seq:cat)
     gene            complement(282..355)
                     /locus_tag="ACGE4H_002860"
     tRNA            complement(282..355)
                     /locus_tag="ACGE4H_002860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Met,seq:cat)
     gene            complement(383..455)
                     /locus_tag="ACGE4H_002861"
     tRNA            complement(383..455)
                     /locus_tag="ACGE4H_002861"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Ala,seq:tgc)
     gene            complement(475..548)
                     /locus_tag="ACGE4H_002862"
     tRNA            complement(475..548)
                     /locus_tag="ACGE4H_002862"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Pro,seq:tgg)
     gene            complement(558..631)
                     /locus_tag="ACGE4H_002863"
     tRNA            complement(558..631)
                     /locus_tag="ACGE4H_002863"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Arg,seq:acg)
     gene            complement(642..727)
                     /locus_tag="ACGE4H_002864"
     tRNA            complement(642..727)
                     /locus_tag="ACGE4H_002864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Leu,seq:taa)
     gene            complement(744..815)
                     /locus_tag="ACGE4H_002865"
     tRNA            complement(744..815)
                     /locus_tag="ACGE4H_002865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Gly,seq:gcc)
     gene            complement(827..899)
                     /locus_tag="ACGE4H_002866"
     tRNA            complement(827..899)
                     /locus_tag="ACGE4H_002866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Thr,seq:tgt)
     gene            complement(917..998)
                     /locus_tag="ACGE4H_002867"
     tRNA            complement(917..998)
                     /locus_tag="ACGE4H_002867"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:tag)
     gene            complement(1006..1078)
                     /locus_tag="ACGE4H_002868"
     tRNA            complement(1006..1078)
                     /locus_tag="ACGE4H_002868"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Lys,seq:ttt)
     gene            complement(1082..1154)
                     /locus_tag="ACGE4H_002869"
     tRNA            complement(1082..1154)
                     /locus_tag="ACGE4H_002869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:tac)
BASE COUNT          303 a          331 c          278 g          253 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catatgaaac
      181 ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt tatgagccgt
      241 ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg aggggatcga
      301 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgcgaaaaa
      361 gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac ctcctgcgtg
      421 caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata attatcggga
      481 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      541 acttcccgtt taattaacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt
      601 caaacactct atccagttga gctatgggcg catatttgaa atgccgagga ccggaatcga
      661 accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      721 ccccggcgcg attgctttgg caaagcggaa aacggggttc gaacccgcga cccccacctt
      781 ggcaaggtgg tgctctacca ctgagctatt tccgcgtgtt taaagatgcc ggctaaagga
      841 cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc taagccggca
      901 tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa ggcgctagat
      961 cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc cgtacagggc
     1021 tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct aacggctcat
     1081 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
     1141 tgagctaaac ctccatggat aactt
//
LOCUS       DAXWLB010000301         1147 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXWLB010000301 DAXWLB010000000
VERSION     DAXWLB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.309"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..254
                     /locus_tag="ACGE4H_002870"
     CDS             <1..254
                     /locus_tag="ACGE4H_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366209.1"
                     /translation="QQKKLIYLINSKNFNTTLSNLIEMDAKISDYLFFLINCRFENDK
                     EVIECVESVEKDGLRDYYCGLNFSDNINNYKLIVHTTKY"
     gene            818..1054
                     /locus_tag="ACGE4H_002871"
     CDS             818..1054
                     /locus_tag="ACGE4H_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366210.1"
                     /translation="MDKGVADIAKIKQVLKQESIKSLVEGTGLSKSTISSLKSGTRKV
                     EKLNLFAAIKLTEYSDQVFKPIIEIWGKELKKQL"
BASE COUNT          453 a          118 c          184 g          392 t
ORIGIN      
        1 agcagcaaaa aaaattgatt tacttaataa actcaaagaa tttcaatact acactaagca
       61 atttaattga aatggatgcc aaaatttctg attatctttt tttcttgatt aattgtagat
      121 ttgaaaatga taaagaagta atagagtgtg tagaaagtgt agaaaaagat ggtcttagag
      181 actactattg tgggttaaat ttcagtgata acataaataa ctataaattg atagttcaca
      241 caactaaata ttaatttttt tagaacgatg ttaaaagatg gatgatatag gggattagtt
      301 attttaacta gtatttattt aggagaaatt ttagaaagaa aagatcgatt atttatcctg
      361 agttagaagt agtaaccttt cggtttaatt ttaatgataa agtgatattt actcaaaatt
      421 taaaaacaaa taatgaaaat gaaaagcttt atgaaccaac aaatattgat acccaagatg
      481 aaaaaactaa taatacaaca aaagtttatg acggttggta ttatttaggc tctttgtcaa
      541 ataggactga tagagtgata taagaacatc ggctctataa ttatgagtat tatgctggat
      601 tagacgtcca tcaaaataat tttttgtttt gaaaggttcc tttattcaga taaataggat
      661 gttggagtat atctatgttg cttatgtaat acaatttatt tttattgtat atttgttccc
      721 ttttcgtact tttaaacttt tactaccaga gcatactaaa atatgctata tttttactac
      781 agtagttata aatttgagat aaaaaaggag tctttttttg gataaaggag tcgctgatat
      841 tgcaaaaatt aagcaagtgt tgaaacaaga atctataaaa agcttagtag aaggaactgg
      901 tttaagtaag agtactattt caagtttaaa atcaggtact cgaaaagtag aaaaattaaa
      961 cctttttgca gctattaaac taaccgaata ttcagatcaa gtttttaaac ctattattga
     1021 aatctgggga aaagaactaa aaaaacaact atagttatcg aataaaacta atagatttaa
     1081 aaatagaaga actagatatt tttcagatga atgcttgttt agctctacaa tgggtagaac
     1141 tgaaaga
//
LOCUS       DAXWLB010000302         1125 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXWLB010000302 DAXWLB010000000
VERSION     DAXWLB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.310"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..547)
                     /locus_tag="ACGE4H_002872"
     CDS             complement(<1..547)
                     /locus_tag="ACGE4H_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8366211.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKL"
     gene            882..>1125
                     /locus_tag="ACGE4H_002873"
     CDS             882..>1125
                     /locus_tag="ACGE4H_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366212.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT          414 a          209 c          170 g          332 t
ORIGIN      
        1 tcaatttttt tgtattttcg taataattat cgattgccac tgcttcaacc ttatgtttat
       61 tgtttttctt agattgaaaa ttttggatag ctggtgcgac atctttctcc aaacccacga
      121 taaggacttt cttatcacga ctgactctta agtacagttt gtaaacgact atccggtaaa
      181 cactaagtaa tatcgtacct aggataaacg agatcaaaat gacgccacgc ggaaatgcaa
      241 accatcggcc agcaaaagta actaccatga tccccaatgc tattagtact tgtccaataa
      301 cagtaatgaa gacaatatca ctgatcattc ggttatagaa tacataagct cctaacaaaa
      361 tattcaacgc aataaagatg atggaaataa aaattgctga atgctgaaag gtttctaagt
      421 ttctcactgg tacttctccc tggaatttca gcaaaaaact aagatagatc gatacattta
      481 atacaagtac atccataaga ataatgaata ttctgcgtgc cgcattccac tccccatttt
      541 tattcatata tacactccta accataaaat aatgcctcta tcatcttacc atagcaaagt
      601 ttaaaaacct agtgaataac caaattgtaa aggaaattaa aagaaacggg gaaaagcgtt
      661 tattgtctgt ttatatgtgt atttctgcat aacagaaagt tgtctcagaa gtcccatcac
      721 ttcctttatg ctgaactagt attctcctgc taattcctta ttcacaagtt acaatattca
      781 tataacgcga attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa
      841 ttgtatttcc gacgaaagaa aacaaaggag tgtaaccata aatgacctac aaacatctta
      901 ccatagacga actgacaatg atagaatcat attatcttca acataataag ccggttgaaa
      961 tcgctaaccg aatgggacgt gctatacaaa ctatttataa tgtagtcaat aagttcaagc
     1021 aaggcaagac tgctcttgat tattggcacc agtataaaga aaataagaaa aaatgtggta
     1081 gaaaagtcat tcaattacct gctcatgaag tagattacat taaag
//
LOCUS       DAXWLB010000303         1119 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXWLB010000303 DAXWLB010000000
VERSION     DAXWLB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.311"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(34..405)
                     /locus_tag="ACGE4H_002874"
     CDS             complement(34..405)
                     /locus_tag="ACGE4H_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366213.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(407..808)
                     /locus_tag="ACGE4H_002875"
     CDS             complement(407..808)
                     /locus_tag="ACGE4H_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366214.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(822..>1119)
                     /locus_tag="ACGE4H_002876"
     CDS             complement(822..>1119)
                     /locus_tag="ACGE4H_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366215.1"
                     /translation="IPTALSFSVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVS
                     VSLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          350 a          205 c          141 g          423 t
ORIGIN      
        1 aaaaaaataa caaacaaact cgcttgaaat tcattaggtg agtttgttta ttattttttc
       61 tgcttctcga ttcatcagcg ccatatctgt tttcgtaatg gtttcattag gaatatagtc
      121 agcacatttt cttttctcta acattctata cccactatta tgtattccta aggtattcaa
      181 ttccttaact gttgaaaaca aattccaaac ggtttggtgg cttccttttc cgctacttcc
      241 attataaata atatcataat tttctttcaa atactcatca gattttttaa aagcactgta
      301 gtatgctctt gaaacagcag ttcttctata agattcttta gaattatatt tatcatctga
      361 ttcaatttgt ttggaaatag ataaatattc attaaagtca aacattttat tcccccaaag
      421 ttgctactac agatttcccc atatatttat agtcagataa tacattatcg agagtatcca
      481 aaacatcgta atctgaggaa aacaattcag atgttgtttt gataacaaag aaatacaagt
      541 ttaaatcttc atcatatttt atattgattt cagcttttct ttttaaaacc gtatttatca
      601 catcaaaaat tttgccatca ttacttgaaa ttgtgttaat ctcatttttt gtagtaatta
      661 caagttgatt atcattttta gttttcggag tagaacttgc atccacaaca actggagatg
      721 ctagaattac cgatgcgcaa gcaaaaatcg cagcaaaatt tattaagcta ccactccgat
      781 gtgtatttga tggtactata tcaagcatcc gtatcgtctc cttaattttt ttccactggt
      841 ttgttgacct tgatatcaaa gatttgcctt ccaatctcag ttccattaaa tttaacaaca
      901 gcataatgct ctcctgcttc ttcaaccaag acatttctgg cgtttatatt aaagtttata
      961 tcgttaactt gaggaggcaa gcttaccgat acttcacccg tagaaaaaat ttgtttttca
     1021 ttcttgttta aaacttcaat ttctactatt tttatctttt ctgtatcctc tatctcagac
     1081 accacagctg ttacagaaaa tgataaagca gtaggaatt
//
LOCUS       DAXWLB010000304         1103 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXWLB010000304 DAXWLB010000000
VERSION     DAXWLB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.312"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            2..1084
                     /locus_tag="ACGE4H_002877"
     CDS             2..1084
                     /locus_tag="ACGE4H_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8366216.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          401 a          192 c          213 g          297 t
ORIGIN      
        1 tatcaatgat gatggctcaa gaaaaaaagt ggagaaagtt ggcggagaca cccgaccaga
       61 ggccgaagct gctttacgaa aagttttatc agatattgac gaaacaggac aatacttttt
      121 aggtacggat actcgagtaa aacaatacct tgatttttgg atggaggaat acgttaaact
      181 aaatctaaaa tacaatacct atgaaaacta ccgatttacc atcaaaaatc atataaacgg
      241 ttatttagga aagaaaaaac ttacggatct ctcccctgct cttttacaaa atttcatcaa
      301 tgctgaattt aaaaagggtt actcgaagaa aacaatgact attactcact ctgtccttaa
      361 gaatgcgctg aatatggcgg tttatccttg gggattaatc aagcaaaatc ctatgctgta
      421 tgtaaaaata ccaaaatatg aagcacgacc aacgactaaa aaagatctaa aaatcatttc
      481 tcttgaggac tttgatcata tgctagaaat tactcctgaa ggacatcctt tctatattcc
      541 tttgaatatt ggattttata cgggaatgcg cgttggcgaa gtttgtggtc tgacgtggga
      601 tgatgtcgat ttttcaaatg gaacaattac tgtagagaaa caaatggtaa agaatgacgg
      661 cgcatgggta tatggtacgc caaagacaag cagttccaat cgaacgattt ttattggaca
      721 aaccttgcta gcaattctga aaaaacataa gaaacaacaa ttagaaaatc gaatgaagta
      781 tggaaagctc tacattgatt caaatgcagt atgtacgaag gaagacggtg agctagttac
      841 gccaagtgta gtgaaatgga atactaggag gatatcgaat gcactctccc tctcttttaa
      901 cttccattct ctcagacata ctcatgctac acttcttctc gaaaatggcg caaaaatgaa
      961 agaaatctct gaacgattgg gccacagcag aatttcaatt acgatggata cttactcgca
     1021 tgtaacagat aagatgagaa atgaaacggt cgatatcatg gaaaatctta gaaagaattc
     1081 ttgatttttg ccaccgaaaa aaa
//
LOCUS       DAXWLB010000305         1101 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXWLB010000305 DAXWLB010000000
VERSION     DAXWLB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.313"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1074)
                     /gene="dnaN"
                     /locus_tag="ACGE4H_002878"
     CDS             complement(<1..1074)
                     /gene="dnaN"
                     /locus_tag="ACGE4H_002878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFF8366217.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLE"
BASE COUNT          340 a          205 c          203 g          353 t
ORIGIN      
        1 ttccaatgta aatggacgaa tagcagagat aaatttcact ttgatgctca tatctccaaa
       61 tgcacgcaac gctgctttca tataatccgg attgaaagaa atatctaatg gatcgccact
      121 gcttgctgta taactcaagc tttcttctac tttcccgatt tctggcgaat ttccgtataa
      181 aacaaccgca tctggacgaa tagataaacg aacgatgttg ttacggcctt catgagaaag
      241 taaagaagcc cgttcgatcg ccgctaaaaa gctaggaaca gaaaattcaa cctctgtatt
      301 aaaacttgaa ggaatcaaac gattggtatc tggatagttt ccttctagca aacgggaata
      361 gaaatacatc gtttctgttt tgaacaacac ttggttttcc ataatgctga tttcgacgat
      421 ttcttcttca tttgttaatg agcgagataa ttcgatcaaa ctttttccag gaataacaat
      481 atcaaaatga tcagccgctt gttctactgg aatcacgcgt tgacttagac ggtgagaatc
      541 agtagctaca gctaataaag aattatcaga taaaatgaaa tgcacacctg tcaagattgg
      601 acgactctca tgttgtgaga cagcaaaaac tgtttcgttg attagtttag tcaatacgtg
      661 tacaggtaat ttcatctggt tatggctttc gacaacagga agatgaggat agttatctgc
      721 atctaatcca tttacgataa aattcgcttt tccagaagtg atcgctactt gtttattttc
      781 taaaacttct aaagtaaatg tttcttcagg aagtctccga atgatttcgc taaagaaacg
      841 tgcttgtaaa acaatggaac cagtagattc gatttgcata tttgcttttt cgttttcaac
      901 agacaaaaaa gtttcaattg atatatcagc gttgctcccc gtcaaagtca aaccttcttg
      961 tgtcagtgtg atttttacac ctgtcaaaat agggatcgtg gttttgcttg aaatagctcg
     1021 ttgaacagtt tgcaattcct gcataaagct agctcggttt aaagtaactt tcatgatgta
     1081 tctcctttga atgtttgttt a
//
LOCUS       DAXWLB010000306         1076 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.315, whole genome shotgun sequence.
ACCESSION   DAXWLB010000306 DAXWLB010000000
VERSION     DAXWLB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.315"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(61..285)
                     /locus_tag="ACGE4H_002879"
                     /pseudo
     CDS             complement(61..285)
                     /locus_tag="ACGE4H_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(839..925)
                     /locus_tag="ACGE4H_002880"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ACGE4H_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          373 a          185 c          156 g          362 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gctttt
//
LOCUS       DAXWLB010000307         1073 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXWLB010000307 DAXWLB010000000
VERSION     DAXWLB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.316"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>1073
                     /locus_tag="ACGE4H_002881"
     rRNA            <1..>1073
                     /locus_tag="ACGE4H_002881"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAXWLB010000308         1070 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXWLB010000308 DAXWLB010000000
VERSION     DAXWLB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.317"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(75..959)
                     /locus_tag="ACGE4H_002882"
     CDS             complement(75..959)
                     /locus_tag="ACGE4H_002882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFF8366218.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
BASE COUNT          294 a          253 c          169 g          354 t
ORIGIN      
        1 caaaagtctt gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg
       61 tcacaacctc cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca
      121 ggtaatttat ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat
      181 gacacaacta attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct
      241 tttaataggt ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc
      301 aaatggagct taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact
      361 gagttagtta gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact
      421 tctacgtgaa acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa
      481 gctagttttc caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata
      541 gagccaattg cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc
      601 ttgactgatt caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat
      661 gctcgagcca agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct
      721 tgttcagcga ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca
      781 ctatgatacc cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt
      841 gtatgcaaga cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca
      901 aattcctttg cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc
      961 actttctttc ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag
     1021 gctgtgatga agtactgttc atgaaacatt catttatttt ttgacaagat
//
LOCUS       DAXWLB010000309         1068 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXWLB010000309 DAXWLB010000000
VERSION     DAXWLB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.318"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            84..1028
                     /locus_tag="ACGE4H_002883"
     CDS             84..1028
                     /locus_tag="ACGE4H_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFF8366219.1"
                     /translation="MDTILITGGAGFTGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
BASE COUNT          365 a          156 c          216 g          331 t
ORIGIN      
        1 tcaacaaatt ttatagtttt tacaatataa aaatggtaaa ctaatattat ttgatacgaa
       61 aatgtgcaat tggagtgaat ttgatggaca ctatattgat tactggcgga gctggtttta
      121 ccggttcaac gctagcgaat tatttgggaa aagaaaacaa aatcgtagta gttgacgatt
      181 tatcaatggg aaaaaaagaa aacttggata tggataaaca agtgactttc attgaggggg
      241 atgtatcaga ttctcaattg atggaaagaa tcatgaaaga atatcagttt gcctatattt
      301 ttcatctagc tgctgtagct agtgtggcag attctgtaga acggcctgta gaaacacatc
      361 gtgtaaattt tgacagtgcg cttttattgc ttgagttagc acggaaatat caatctaatt
      421 taaaacgatt agtatttagt tcatcagctg cagtttatgg tgatgaacca acactgccaa
      481 aaaaagaaga atcagtcatc cgtccattaa caccatatgc tatcgataaa tttgcagcag
      541 aacaatatgt tttggattac tgtcatttat atgatgttcc gactagtgct gtccgatttt
      601 ttaacgttta tggtccaaat caaaatccaa actctccata tagcggagtc atttcgattc
      661 tagtggatcg ttacaaaaaa caattggcag gagaaaaagc ggaatttact ttatttggcg
      721 atgggagtca gtctagagac tttgtttata tagaagatgt tatacaagca cttttacttg
      781 ttgccaagga agaaaaagct ttaggacagc aattcaatgt cggtacagga aaaagcacta
      841 cgctacttga attgatccat tcaattgatc aaatcttagg aacagagcta gcgcttaaat
      901 atgaagcgga gcgttcaggg gatattcgtg attccttagc ggatatttca aaaattcgtt
      961 ctttaggcta tcagcctaaa tttgatatat tgagcggaat ggaacggtat ttaaagacag
     1021 aaatttgatg aaaaaatgat tgcatctttg gataaataat tctaaaaa
//
LOCUS       DAXWLB010000310         1053 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXWLB010000310 DAXWLB010000000
VERSION     DAXWLB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.319"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            110..964
                     /locus_tag="ACGE4H_002884"
                     /pseudo
     CDS             110..964
                     /locus_tag="ACGE4H_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          376 a          165 c          202 g          310 t
ORIGIN      
        1 taaaagatag cgtaaaatat tttgtgtaaa tagaaattta gggtagaaaa aaaagtccat
       61 tctgtaaaat taaagttacc acacagaaat tttataggag gactttctca tgactaaatt
      121 tactatagaa attatgcaaa cactactcaa taaaggggat ttagatgaat tatttcagga
      181 tcatttagag cgagcgatta attcacttct acaagcagag ctaactgctt ttttagatta
      241 cgaaaaatat gatcgaaatg gattcaattc aggaaattcc cgtaacggga attattcacg
      301 tacatttaaa acagaatatg gcgagttgaa tttaactatt cctagagatc ggaatggcga
      361 attttctcaa caaacactac cagcatataa gcgaaccaat gattcattag aaacgaccat
      421 tatccaatta tttcaaaaag gcattactat ggctgaaatc tccaaattaa ttgaaaaaat
      481 gtatggtcac cactatacgc cacaaacaat ctccaacatg agtaaattgg tggctgaaga
      541 tgttttagct tttaaagaaa gaacgttaga agccaattat tccgttatat ttatggatgc
      601 gacgcatatc cctgtgaagc gacagactgt ttcgaaggaa gcagtgtata ttacgattgg
      661 tattcgtttg gatggaacca aagaagttct tgggtttact attgccccaa ctgagtctgc
      721 ttatatttgg aaagaagttc ttcaagatct tagaaaacgt gggttagaag aagttttatt
      781 agtagtgaca gacggattaa gctgtattga agaaagtatc catagtgtgt atccgaatgc
      841 ccaatttcaa caatgttgtg tgcatgtatc tagaaatatc gctcataaag ttcgtgttcg
      901 aggtcgaaaa gaaatttgtg aggatttcaa attggtttac caagcgaatt caaaagaaga
      961 ggcgtaagcg ccccatagaa cagtacctta ctaacaaatg cacctcaaga tatacttgag
     1021 ctatcaagaa gtctggaggt tttctatgta taa
//
LOCUS       DAXWLB010000311         1042 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXWLB010000311 DAXWLB010000000
VERSION     DAXWLB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.320"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(58..933)
                     /locus_tag="ACGE4H_002885"
     CDS             complement(58..933)
                     /locus_tag="ACGE4H_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFF8366220.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT          319 a          205 c          148 g          370 t
ORIGIN      
        1 catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc ccttatttta
       61 atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa tggcaagttg
      121 aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt tgctatgttt
      181 aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat aaatcaagcg
      241 tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt ctttatcata
      301 aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt ctactatttt
      361 taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca tagtgatgaa
      421 tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct cgaccacttc
      481 tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca taattgctgt
      541 gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg tcaatgtaat
      601 atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa tcacttcttc
      661 cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc gtttgatctt
      721 ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg gcttactcac
      781 tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat cgtcatcgat
      841 caatacagca ttatgattca gctttttcat cacatacacc gttaattcac ctcctctata
      901 tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca aacaattttt
      961 aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat tgaatagtag
     1021 caccctacat aatttacaaa aa
//
LOCUS       DAXWLB010000312         1025 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXWLB010000312 DAXWLB010000000
VERSION     DAXWLB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.321"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..890)
                     /locus_tag="ACGE4H_002886"
     CDS             complement(<1..890)
                     /locus_tag="ACGE4H_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFF8366221.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPK"
BASE COUNT          325 a          177 c          125 g          398 t
ORIGIN      
        1 aattttggtg aaaagtcggg atcaattttc acttcttttt caaagcgttg taaaatattt
       61 atagaacgta aataatcttc tttattggta tagttatttt taatcattgt catcatcatc
      121 cttattgctt catagacata gcgagttcta acattaattg ataattccgg ataaaactta
      181 tctatgaata ctagtatatc ttcatggact tcaaaagaat caaaaattct aggattgaag
      241 cttcctctca gtatactttt agggctttgg taatagtgat atttagcaga tgaattgaca
      301 gcaacttttt cggaatgatc gatcaacaaa taagtagtag ccttatcttc cattaattta
      361 cccagtggga atcttatatt atcaaataaa tttctattga ataatttatt atttgcagaa
      421 tagccaattt tattatttat aaataagttt ttcattgctt gttctctatc aaatatttcc
      481 cattttctct cagcaattgt atgttctttt agttgttcta aacttgaaat cttttttatt
      541 tttagcaccg agatatctgc tccataagtt tgcgtaccaa aatatagtaa ctcaagatag
      601 tctggttcta tccaatcatc gctgtcaata aagcacacaa aatctcctgt tgcagaatca
      661 agaccagaat tcctagccgc acttaaacca ccattcactt gatgtatcac ttttaatcgc
      721 atatctttct ttttatattc ttctgcaatt tccccacttc gatccgggga accatcatcg
      781 actactatta tttcaatatt tgaatgtgtc tgattggaaa tagattctaa gcagttttct
      841 aaatagtttt ctacgttata tgcgggtact ataacactaa ttttatgcac tctcttcact
      901 tcctaaaaat catctttttt atttttacaa atgtatcatt accagtaaca attttagtct
      961 tccgaagtta ataaacataa aaaataacat atcgaaaaaa actttatttt tttagaactt
     1021 tttta
//
LOCUS       DAXWLB010000313         1023 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXWLB010000313 DAXWLB010000000
VERSION     DAXWLB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.322"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(178..882)
                     /locus_tag="ACGE4H_002887"
     CDS             complement(178..882)
                     /locus_tag="ACGE4H_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366222.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          291 a          183 c          187 g          362 t
ORIGIN      
        1 cttcaggtag aagagaagtc gcctaacatt gtaggatcat aggtgatttg ttggacaggt
       61 caagctgata tttgagcctg atgttagaag gaatttcttt ttacttgctg tagaaattat
      121 tatatgacta aaaaggctgg gattttaaat cccagccttt tatgacaaat agaattttta
      181 tcgatcagta atcgctgtcg ctgcaaattg ttcgatcaaa ggaatcaaag tcaatttgac
      241 tttgtatttc cgttcgagtg ctttttcgat aacgaactca ttttcagtag tcatagaaat
      301 agttgctaat ttacgaggtt catcagcatt tttttcatta ataactactt catgttcatt
      361 ttgttctgtg taaaatttag gatactcagc aacggctttt gaaaaatcag caaaacttaa
      421 agcggcattc gtatcagttg agcgtgtatt ttcagttgtg tccgcttttg gtttttccgt
      481 tttttcttct ggcacactga ctgtttcttc tgtaactggt gtcgcaacag cttgcgcagt
      541 tggttcatcg tttttcaatc gttgatcatt ccagtaagac acgatttcaa taagaagatt
      601 gtcacggaat cgttcgaaaa tcccgttttc tcctgaacga attctcttta atacagtatt
      661 gaagttgtca tcgattggca cgttgatccg tgtatcttca tttgttttcc cagctaatcc
      721 gatcgttaag atttgacggt ttttgggatc aagcatgact tgtaacggca aaccataacg
      781 tcgcaagctc tcttcttcta gagaacggaa taattgtcct cttacaccat ccaatgaagg
      841 acggaattga tttaataatt tttgtttctg ttttttcttc atatgttctc tccttcgctc
      901 ataccctttc gattataaga aaaaaacagc caaatgaaaa ggagtaatgc aaaaaaactt
      961 gcgtatacgc aaattacaac agaaaaattt gcgtattttc ccgttttttg aaagaaaaat
     1021 aaa
//
LOCUS       DAXWLB010000314         1012 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXWLB010000314 DAXWLB010000000
VERSION     DAXWLB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.323"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..1005)
                     /gene="tet"
                     /locus_tag="ACGE4H_002888"
     CDS             complement(<1..1005)
                     /gene="tet"
                     /locus_tag="ACGE4H_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8366223.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     S"
BASE COUNT          369 a          207 c          152 g          284 t
ORIGIN      
        1 gctaacagaa agaaatgtaa ctccgatgtt taacacgtat aaaggacctc ttctatcaac
       61 aagtatccca ccaatgtagc cgaaaataat gacactcatt gttccaggga aaataattac
      121 acttccgatt tcggcagtac ttagctggtg aacatctttc atcatataag gaaccataga
      181 gacaaaccct gctactgttc caaatataat tcccccacaa agaactccaa tcataaaagg
      241 tatatttttc cctaatccgg gatcaacaaa aggatctgtt actttcttga tatgttttac
      301 aaatatcagg aatgacagca cgctaacgat aagaaaagaa atgctatatg atgttgtaaa
      361 caacataaaa aatacaatgc ctacagacat tagtataatt cctttgatat caaaatgacc
      421 ttttatcctt acttctttct ttaataattt cataagaaac ggaacagtga taattgttat
      481 cataggaatg agtagaagat aggaccaatg aatataatgg gctatcattc caccaatcgc
      541 tggaccgact ccttctccca tggctactat cgatccaata agaccaaatg ctttacccct
      601 attttccttt ggaatatagc gcgcaactac aaccattacg agtgctggaa atgcagctgc
      661 accagcccct tgaataaaac gagccataat aagtaaggaa aagaaagaat ggccaacaaa
      721 cccaattacc gacccgaaac aatttattat aattccaaat aggagtaacc ttttgatgcc
      781 taattgatca gatagctttc catatacagc tgttccaatg gaaaaggtta acataaaggc
      841 tgtgttcacc cagtttgtac tcgcaggtgg tttattaaaa tcatttgcaa tatcaggtaa
      901 tgagacgttc aaaaccattt catttaatac gctaaaaaaa gataaaacgc aaagccaaat
      961 taaaatttgg ttgtatttaa ccctgttaca ttcattacac ttcataatta at
//
LOCUS       DAXWLB010000315         1010 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXWLB010000315 DAXWLB010000000
VERSION     DAXWLB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.324"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            73..>1010
                     /locus_tag="ACGE4H_002889"
     CDS             73..>1010
                     /locus_tag="ACGE4H_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8366224.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSI"
BASE COUNT          352 a          173 c          187 g          298 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataatattcc tagaaaatca
      961 ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga
//
LOCUS       DAXWLB010000316          999 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXWLB010000316 DAXWLB010000000
VERSION     DAXWLB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.325"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            10..420
                     /locus_tag="ACGE4H_002890"
     CDS             10..420
                     /locus_tag="ACGE4H_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8366225.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="ACGE4H_002891"
     CDS             434..790
                     /locus_tag="ACGE4H_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFF8366226.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          340 a          157 c          194 g          308 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
      961 tatattgaca tgtgtaaatt tacatgatat attcatttc
//
LOCUS       DAXWLB010000317          978 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXWLB010000317 DAXWLB010000000
VERSION     DAXWLB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.326"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..536
                     /locus_tag="ACGE4H_002892"
     CDS             <1..536
                     /locus_tag="ACGE4H_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFF8366227.1"
                     /translation="SIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSET
                     PKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFH
                     ILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSH
                     KLISYPIIEKQQIKYIN"
BASE COUNT          339 a          126 c          157 g          356 t
ORIGIN      
        1 aatcaattgt cattgaaagt atgaaagatg gacttcatat atatgataac cctgtgggcg
       61 ttcttaccaa taatccttca tttgactatc aattatttaa tttaaacaat tatcgtgtct
      121 tatcgagtga aactcctaaa aataattttt caaatcaaat aagtttgaat gcctatagcc
      181 gcggtatggg agggataggc ttgcctggag atttatcctc agtatctcgt tttgttaaag
      241 cgacttttac gaagctgaat tctgtatctg gagattcaga gtcagaaagt attagtcaat
      301 ttttccatat cttaggttca gtagaacaac aaaaaggttt gtgtgatgtt ggtgatggaa
      361 aatatgaata tacaatttat tcttcttgtt gcaatgttga caaaggaatc tattattatc
      421 gaacatatga agacagtcaa attactgcaa ttgatatgaa taaagaagac ttagatagtc
      481 ataagttaat tagttatcca attatagaaa aacaacaaat taaatatata aattagttaa
      541 tgtgttgtga ttgattattt aatatagata taatacaaaa aggcaaagat attttcaaaa
      601 cattttttct tgatgtgata tccttgtctt ttttgtaatg tttttaactt attgcttttg
      661 caaaatttat ttacctgttt tctggatcaa cgatagtgta tattatgcta tccttgtttg
      721 gattttagga cgtttaaaga ggcacttttt tacataactt aatccataga ccttttaagc
      781 atgctgtgag ataataataa agaagagttt aaagcgcacc ccaaaccact tccccataag
      841 tgtattacgc tttaaactct tttatatttg aattcactaa gaagcatacc atatttttga
      901 aaaaaagtga gaaaaaaggc ttataattag gatttagagt aattaattag atgtttattt
      961 tataggactg atactata
//
LOCUS       DAXWLB010000318          961 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXWLB010000318 DAXWLB010000000
VERSION     DAXWLB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.327"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            263..496
                     /locus_tag="ACGE4H_002893"
                     /pseudo
     CDS             263..496
                     /locus_tag="ACGE4H_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(544..663)
                     /locus_tag="ACGE4H_002894"
                     /pseudo
     CDS             complement(544..663)
                     /locus_tag="ACGE4H_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          349 a          195 c          126 g          291 t
ORIGIN      
        1 tgcccaacaa attcatagga ggattcaaga tgtctcatac tctgcttatc aaagataccc
       61 taaatattct agacctaaat atcaatatcc atttcgagga aaattgttta actaaagaaa
      121 aatacaaagg acaaatttgc ttgatttaca aagggactct tcagtacaca ccagaagaat
      181 atccacatct tccaatatat taaaagaacc ttcttgaatc attgagtgaa agaaaagaga
      241 ttcgtcacta tttgaggact gtttaactag agacattagc gatctgcctg aaacctatac
      301 aaccacttta cgaacgttta aaaattttct gccacagatt caaaatgcgc ttaactgcag
      361 ctattcaaac agaccattgg aatgtttaaa caaccacatc aaagtattaa aacgcaatac
      421 gtatggcttt cgcagctttt acaatttcaa actacggatc atgattcgtc atggcaaaac
      481 atttttaacc aaataaacag aagaatgtgg gataaatcta aaaatcagtt ttgctacaga
      541 ctctcgagta aacggcggga gcagaagcaa ctccttcgga aataagccga aattcacaaa
      601 aatttgaaaa gcaattttcg tgaatttctt cttatttctc ggagttaaac acttctgtcc
      661 cagcctcttc tgtccaaatt attctaagat ttctctttac caacatcatt tgacaaagag
      721 cttctttttt gaatactata ttcgttctgt ttttcatctt caaatacgaa ctcatccgca
      781 gaaatatttg tcacaactcc atgaatcttc ttttcattac cacctatata ccacaggatt
      841 cctacacaga aacaacaaaa aaatagcata aaataggttt tctttatttt atatccctcc
      901 ttttagatta aaagctcaca gaaaacgctt tctaaaaaga caatagaata atcataattt
      961 t
//
LOCUS       DAXWLB010000319          936 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXWLB010000319 DAXWLB010000000
VERSION     DAXWLB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.328"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..436
                     /locus_tag="ACGE4H_002895"
     CDS             <1..436
                     /locus_tag="ACGE4H_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFF8366228.1"
                     /translation="DRLNTLKKIKAFDINICSGFICGMGETDTQLVDLAFELKEQEPF
                     SIPVNFLLPIKGTKLEKYNELTPIKCLKILIMLRLLFPKTEIRISAGREFHLGSLQQF
                     AILIVDSIFLGNYLTEKGADVTEDYQLLKELELIVEGDYNEY"
     gene            426..>936
                     /gene="bioD"
                     /locus_tag="ACGE4H_002896"
     CDS             426..>936
                     /gene="bioD"
                     /locus_tag="ACGE4H_002896"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFF8366229.1"
                     /translation="MNISMVICGIDTNCGKSVASILIYEYLKLYFSVNPIIVKPVQTG
                     QELDTIFYQQCDIPQKNIFNFYSLDIEASLHTAGNLTNQKIDLQSLLDQSKALLSDNR
                     PKIFELAGGILSPINDKETMLDFVKLLNLPIILVVDNYLGSINHTLLTIHLLESKNIE
                     ILGLIYNNTE"
BASE COUNT          354 a          129 c          137 g          316 t
ORIGIN      
        1 cgatcgttta aatacgttaa aaaaaatcaa agcttttgac ataaatatct gctctggctt
       61 tatatgtgga atgggagaaa ctgatacaca attagtcgat ttagcttttg aattaaaaga
      121 acaagagcct ttttcaattc ctgtaaattt tttattaccc attaaaggaa ccaaacttga
      181 aaaatataat gaactaaccc caataaaatg tctaaaaatt ctaataatgc ttagactatt
      241 gtttccaaaa acagaaatta gaataagtgc tggtagagaa tttcatttag gcagtcttca
      301 gcaatttgct attttaattg tagattctat atttttagga aactatttaa cagaaaaagg
      361 cgcggatgtc acagaagatt atcaattatt aaaagagtta gaacttatcg tagaggggga
      421 ttataatgaa tattagtatg gtgatttgtg gaatagatac aaattgtggt aaatcagtag
      481 ccagtatttt aatttatgaa tatctgaaac tctatttttc agttaatcca attattgtaa
      541 agccagtgca aacaggacaa gagcttgata ctatttttta tcaacagtgt gatatacctc
      601 aaaaaaacat ttttaatttc tattcattag atatcgaagc ctcactacat actgcaggga
      661 atttaacaaa tcaaaaaatt gacttacaat ccttgttaga tcaatcaaaa gctttactaa
      721 gtgacaatcg acctaaaata tttgaattag ctggtggaat tttatctcct atcaacgata
      781 aagaaacaat gttagatttt gtcaaattat taaaccttcc aattatctta gttgtagata
      841 attacttagg ctcaattaat catactttac taactatcca tcttttagaa agcaagaata
      901 tagaaatttt gggattgatt tacaataata cagaat
//
LOCUS       DAXWLB010000320          927 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXWLB010000320 DAXWLB010000000
VERSION     DAXWLB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.329"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE4H_002897"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACGE4H_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8366230.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          270 a          192 c          139 g          326 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatga
//
LOCUS       DAXWLB010000321          910 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXWLB010000321 DAXWLB010000000
VERSION     DAXWLB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.330"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..355
                     /locus_tag="ACGE4H_002898"
     CDS             <1..355
                     /locus_tag="ACGE4H_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366231.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACGE4H_002899"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACGE4H_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAXWLB010000322          906 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXWLB010000322 DAXWLB010000000
VERSION     DAXWLB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.331"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            189..773
                     /locus_tag="ACGE4H_002900"
     CDS             189..773
                     /locus_tag="ACGE4H_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366232.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          288 a          129 c          181 g          308 t
ORIGIN      
        1 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
       61 tctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
      121 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
      181 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
      241 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
      301 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
      361 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
      421 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
      481 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
      541 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
      601 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
      661 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
      721 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
      781 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
      841 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
      901 tattaa
//
LOCUS       DAXWLB010000323          903 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXWLB010000323 DAXWLB010000000
VERSION     DAXWLB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.332"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..>903)
                     /locus_tag="ACGE4H_002901"
     rRNA            complement(<1..>903)
                     /locus_tag="ACGE4H_002901"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          193 a          277 c          203 g          230 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 ggg
//
LOCUS       DAXWLB010000324          889 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXWLB010000324 DAXWLB010000000
VERSION     DAXWLB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.333"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..478)
                     /locus_tag="ACGE4H_002902"
     CDS             complement(<1..478)
                     /locus_tag="ACGE4H_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFF8366233.1"
                     /translation="MMNTINTKMTKSDCLEILSSKENIWFYYSQALYTKTKNMKNRIR
                     LNTLLNAKSGLCNEDCGYCAQSKKSKATIQKYSLLPQDEIIRKALIAKKNNSSVFCIA
                     LSGTRPTQKEVLVLGETIKEIKRIMDIEICLSIGLITDNQIRYLKKCGIDRLNHNLN"
BASE COUNT          298 a          119 c          118 g          354 t
ORIGIN      
        1 tatttaaatt atgatttaat ctatcaattc cgcatttttt taagtatctt atctgattat
       61 ctgtgattaa tccaatagat aaacaaatct cgatatccat aatgcgctta atttctttaa
      121 ttgtttcgcc gagaactaat acttcttttt gagtaggtct agttccactt aacgcaatac
      181 aaaaaacact tgaattattc ttttttgcta tcaatgcttt acgtataatc tcatcttggg
      241 gtaaaagact gtatttttga atggtcgcct tacttttttt agattgggcg cagtaaccac
      301 aatcttcatt acataatcca cttttagcat ttagaagtgt atttaacctt attctatttt
      361 tcatattttt agttttagta tatagggctt gcgaatagta aaaccaaata ttttcttttg
      421 aacttaaaat ttctaaacag tcagattttg tcatttttgt attaatagta ttcatcatga
      481 tagtaattct ccttgtagtt ggttaaccaa ttatattata caggttaacg aaaatgaagg
      541 ggtaaattac tgataatgtt taaattttat taataaagga taagcttatc agtttaggga
      601 catagttaaa tgaaaacatt tggaaagctg ctaattcttt aattaaaaaa attttgttag
      661 ttttatcttt atcaagagtt actagcattc aatatattag tttttatcaa ttgctaccta
      721 ctaaaaggcc atttagttaa gagttctagg agatgaatat atcccttttt ctccctaatt
      781 aatcatattt atccgctatt ttaaaaggca aatagtattt aataaagcat acttaaaacg
      841 aagtacattt gctgttagaa taattttgga aatttaagga agaaaaaat
//
LOCUS       DAXWLB010000325          883 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXWLB010000325 DAXWLB010000000
VERSION     DAXWLB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.334"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..861
                     /locus_tag="ACGE4H_002903"
     CDS             <1..861
                     /locus_tag="ACGE4H_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFF8366234.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          377 a          116 c          145 g          245 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gttcaacttt tggggttcag ttc
//
LOCUS       DAXWLB010000326          868 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXWLB010000326 DAXWLB010000000
VERSION     DAXWLB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.336"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(55..127)
                     /locus_tag="ACGE4H_002904"
     tRNA            complement(55..127)
                     /locus_tag="ACGE4H_002904"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:His,seq:gtg)
     gene            complement(130..203)
                     /locus_tag="ACGE4H_002905"
     tRNA            complement(130..203)
                     /locus_tag="ACGE4H_002905"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(169..171),aa:Trp,seq:cca)
     gene            complement(210..290)
                     /locus_tag="ACGE4H_002906"
     tRNA            complement(210..290)
                     /locus_tag="ACGE4H_002906"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(254..256),aa:Tyr,seq:gta)
     gene            complement(292..367)
                     /locus_tag="ACGE4H_002907"
     tRNA            complement(292..367)
                     /locus_tag="ACGE4H_002907"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Phe,seq:gaa)
     gene            complement(403..475)
                     /locus_tag="ACGE4H_002908"
     tRNA            complement(403..475)
                     /locus_tag="ACGE4H_002908"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Asp,seq:gtc)
     gene            complement(485..557)
                     /locus_tag="ACGE4H_002909"
     tRNA            complement(485..557)
                     /locus_tag="ACGE4H_002909"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Val,seq:tac)
     gene            complement(573..644)
                     /locus_tag="ACGE4H_002910"
     tRNA            complement(573..644)
                     /locus_tag="ACGE4H_002910"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Glu,seq:ttc)
     gene            complement(659..748)
                     /locus_tag="ACGE4H_002911"
     tRNA            complement(659..748)
                     /locus_tag="ACGE4H_002911"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Ser,seq:gga)
     gene            complement(788..861)
                     /locus_tag="ACGE4H_002912"
     tRNA            complement(788..861)
                     /locus_tag="ACGE4H_002912"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Asn,seq:gtt)
BASE COUNT          242 a          238 c          199 g          189 t
ORIGIN      
        1 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa aaagggcgaa
       61 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
      121 cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg gagaccgctg
      181 ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac cactgaaccc
      241 taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc atggtggcgc
      301 gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc aactgagcta
      361 ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg acgggaatcg
      421 aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg gaaccagttc
      481 attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag atgctctccc
      541 agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg aacccgtgtt
      601 accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg
      661 gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat ttccaatcat
      721 gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact ctttctcaaa
      781 atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt catgcgctac
      841 taccaactga gctatgccgg aataacat
//
LOCUS       DAXWLB010000327          844 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXWLB010000327 DAXWLB010000000
VERSION     DAXWLB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.337"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            269..589
                     /locus_tag="ACGE4H_002913"
     CDS             269..589
                     /locus_tag="ACGE4H_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFF8366235.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            607..>844
                     /locus_tag="ACGE4H_002914"
     CDS             607..>844
                     /locus_tag="ACGE4H_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFF8366236.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFE"
BASE COUNT          309 a          114 c          147 g          274 t
ORIGIN      
        1 cgtaaacaaa tttaacaact tttaaaaaaa ctgcgttgac taaattaaca tacagtgtta
       61 aaatcagaac atagttaaat aagacatata aacattgatt ttaaaagctt tcttggcggt
      121 tggcatttat taatcaatag tgttttttgt tgtcttttta gttgttaaac ttgtttacaa
      181 gaatgatatt aacacctttt gttaaaatag tcaacgaatt ttaacaaata aagttaaata
      241 ttttttcttg aacgaaagga atattgatat gataacgttt gagagaataa aagagttagc
      301 gaaaaaacag ggaaaatcat taaataaagt tgaagaagac ctaggctatg gtaaaaacgt
      361 cttatataga cttaagaact caaacccctc tacagaacgt ttgcaagaaa ttgctgacta
      421 tttcgatgtc tctgtagact atcttctggg gcgcgaagaa aaagaaaccc ctaaacatgt
      481 ggatttatca gaaaacgata ctgttttttc ttttgatgga aaagaaatat ctaaggagac
      541 aatgcgtaaa gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg
      601 ggttgtatgt atttgaaatt gaaagaaatg ttgagcgaat acaatttgaa agtagtttac
      661 atggaaatga aagagcctgg cttctattat ccgaagccta gaatcatttt tttgaatgaa
      721 aatctatatg gcgaaactgc agaagccttt catttatctc atgaacttgc gcatttcacc
      781 gcttctcatt ttgaatattc agttttgtac gatacttcta caacttttca ctcaaaattt
      841 gaaa
//
LOCUS       DAXWLB010000328          830 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXWLB010000328 DAXWLB010000000
VERSION     DAXWLB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.339"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..830
                     /locus_tag="ACGE4H_002915"
     rRNA            <1..830
                     /locus_tag="ACGE4H_002915"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          178 c          262 g          186 t
ORIGIN      
        1 catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag gataggtagg
       61 agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact acccctgcgt
      121 tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga tgggcagttt
      181 gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt
      241 tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac caacaagtcg
      301 agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg ccatcgctca
      361 acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc acatcgacgg
      421 ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg
      481 ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg tgagacagtt
      541 cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag tacgagagga
      601 ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc tgggtagcta
      661 tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca agatgagatt
      721 tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag gtcaggagtg
      781 gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
//
LOCUS       DAXWLB010000329          820 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXWLB010000329 DAXWLB010000000
VERSION     DAXWLB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.340"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            35..532
                     /locus_tag="ACGE4H_002916"
     CDS             35..532
                     /locus_tag="ACGE4H_002916"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8366237.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..>820)
                     /locus_tag="ACGE4H_002917"
     CDS             complement(569..>820)
                     /locus_tag="ACGE4H_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366238.1"
                     /translation="KNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGH
                     YLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVPLPV"
BASE COUNT          292 a          129 c          146 g          253 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ttttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt
//
LOCUS       DAXWLB010000330          811 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXWLB010000330 DAXWLB010000000
VERSION     DAXWLB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.341"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..672
                     /locus_tag="ACGE4H_002918"
     CDS             <1..672
                     /locus_tag="ACGE4H_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFF8366239.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAXWLB010000331          810 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXWLB010000331 DAXWLB010000000
VERSION     DAXWLB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.342"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..706
                     /locus_tag="ACGE4H_002919"
     CDS             <1..706
                     /locus_tag="ACGE4H_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFF8366240.1"
                     /translation="ELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKV
                     RREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVI
                     RENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDV
                     HAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTK
                     LAKAEQYRRSLELSKD"
BASE COUNT          354 a           82 c          143 g          231 t
ORIGIN      
        1 tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc attactctta
       61 tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac agatgataga
      121 agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata aagaacaacg
      181 tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta atgcagatgt
      241 ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa aagaattaga
      301 agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt atgtgtatgt
      361 aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga ctagaagatt
      421 agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg aatttgacgt
      481 tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc ataatcattt
      541 tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag tttcactaga
      601 cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta caaaattagc
      661 taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat ataaaaagcc
      721 ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat agaaataatg
      781 tggttagaaa aaataaatga caaaaaattt
//
LOCUS       DAXWLB010000332          806 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXWLB010000332 DAXWLB010000000
VERSION     DAXWLB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.343"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            25..450
                     /locus_tag="ACGE4H_002920"
     CDS             25..450
                     /locus_tag="ACGE4H_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366241.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT          295 a          140 c          146 g          225 t
ORIGIN      
        1 atcctatcaa atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg
       61 ggacttttga cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa
      121 acaaccgatt cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg
      181 ctaaaagtac gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa
      241 atggaatgga cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa
      301 aaaacagtgc tgacagatat caagatcact accaaagatg acgaatatat tatttcagga
      361 aaagaaaata aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag
      421 gatgaggatg gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc
      481 cccttctttt ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat
      541 cgatagtcct gattattgta catgtaataa caacaaaaat atccatacag aatttaatga
      601 ccaaggaagc ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac
      661 atttgaatct tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag
      721 caaaactcac tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc
      781 ttcatcagta aaacaaacag tggatc
//
LOCUS       DAXWLB010000333          805 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXWLB010000333 DAXWLB010000000
VERSION     DAXWLB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.344"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(138..428)
                     /locus_tag="ACGE4H_002921"
     CDS             complement(138..428)
                     /locus_tag="ACGE4H_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366242.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..>805)
                     /locus_tag="ACGE4H_002922"
     CDS             complement(546..>805)
                     /locus_tag="ACGE4H_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366243.1"
                     /translation="GVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMIY
                     FIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          339 a          127 c          119 g          220 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tccca
//
LOCUS       DAXWLB010000334          795 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXWLB010000334 DAXWLB010000000
VERSION     DAXWLB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.345"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            136..309
                     /locus_tag="ACGE4H_002923"
     CDS             136..309
                     /locus_tag="ACGE4H_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366244.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          262 a          128 c          101 g          304 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttctt
//
LOCUS       DAXWLB010000335          791 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXWLB010000335 DAXWLB010000000
VERSION     DAXWLB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.346"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..702
                     /locus_tag="ACGE4H_002924"
     CDS             <1..702
                     /locus_tag="ACGE4H_002924"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFF8366245.1"
                     /translation="LNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          344 a           54 c          129 g          264 t
ORIGIN      
        1 ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt
       61 gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt
      121 tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta
      181 agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga taataaacaa
      241 aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata
      301 gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt
      361 aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat
      421 agattaactg gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt
      481 atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta
      541 agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa
      601 tattatccta ttgaaactat tgtttatgga attaaaaata ttaaacagga atttatcgaa
      661 aatggtagaa aagaaattta taaaaggact tataaagatt gattatataa tatatgaaaa
      721 gctattataa aagacattag tattaaatag tttaaaaaaa tgaaaaataa taaaggaagt
      781 gagtcaattc c
//
LOCUS       DAXWLB010000336          784 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXWLB010000336 DAXWLB010000000
VERSION     DAXWLB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.347"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>784
                     /locus_tag="ACGE4H_002925"
     CDS             <1..>784
                     /locus_tag="ACGE4H_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFF8366246.1"
                     /translation="DKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFK
                     NKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVD
                     SLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENV
                     YIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVK
                     FDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIF"
BASE COUNT          351 a           53 c          113 g          267 t
ORIGIN      
        1 gataaaatga tgaaacttga taaaaaaatg agtttaaaag ttagctctaa tgatgaagtt
       61 ggagaattaa aacaacaaat taatgatttg tattctactt tattaagaac aattgacgat
      121 ttggaattta aaaataaaga aattttaaaa ttagaaaagt taaaatatga cttttttaaa
      181 ggtgcatccc atgaacttaa aacccctctt gctagtctta aaataatact ggaaaacatg
      241 aaatacaata ttggaaaata taaagaaaga gatatttata ttaatgaatg tattgaaatc
      301 gttgatagtt taacaaaaaa tatttctcaa atattatcag ttcattctat agaaaatttg
      361 aataatgatg aagaatattt gaaaataaat gatacattag aagacatatt aaaaaagtat
      421 gaaatattag caaatcaaaa gaaaataact gtcaataatt atatattaga cgagaatgtt
      481 tatataggaa aaacagcttt aaagattatt ctatctaatt tgattagtaa tgcggtaaaa
      541 tatactgatg taaatggggt aattaatatt gggatagtta atgattggtt atatatagag
      601 aattcatacg gtaacaataa aatttcgaat atggataaaa tatttgatgt taaatttgat
      661 ttaaataagg aaaatagtaa tggattaggt ttatatattg taagtaatat tctgaataat
      721 tacaatatag aatataaagt attgcaggat gaagaatttt ttatctttaa aataaaaata
      781 tttt
//
LOCUS       DAXWLB010000337          782 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.348, whole genome shotgun sequence.
ACCESSION   DAXWLB010000337 DAXWLB010000000
VERSION     DAXWLB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.348"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..525
                     /locus_tag="ACGE4H_002926"
     CDS             <1..525
                     /locus_tag="ACGE4H_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFF8366247.1"
                     /translation="IWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVI
                     DPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKEL
                     ARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQ
                     DFHDKRWYLNIPKR"
BASE COUNT          255 a          150 c          159 g          218 t
ORIGIN      
        1 atctggggcg atcatacaga tccttacggt tatctttctg aaatgggcgt aagtaaagaa
       61 aaattagcat atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      121 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      181 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      241 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      301 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      361 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      421 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      481 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      541 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
      601 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
      661 tgttgccaca cattctactg gttgattgtt tatggcttta tgtggcaaca accagtaccc
      721 ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
      781 ag
//
LOCUS       DAXWLB010000338          780 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXWLB010000338 DAXWLB010000000
VERSION     DAXWLB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.349"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(119..>780)
                     /locus_tag="ACGE4H_002927"
     CDS             complement(119..>780)
                     /locus_tag="ACGE4H_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFF8366248.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          214 a          179 c          148 g          239 t
ORIGIN      
        1 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg
       61 cccttgacaa tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt
      121 acgctgcctc tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc
      181 tctgttgtcg aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt
      241 tccaccaatg cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt
      301 attgatgcac gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat
      361 acggacaacc cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa
      421 tggtttggtt atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag
      481 aatacggtat ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg
      541 aataaccaat aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt
      601 tccccacgcg aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa
      661 aggtccgatc taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa
      721 aatgagcgat cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc
//
LOCUS       DAXWLB010000339          768 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXWLB010000339 DAXWLB010000000
VERSION     DAXWLB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.350"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            290..703
                     /locus_tag="ACGE4H_002928"
     CDS             290..703
                     /locus_tag="ACGE4H_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFF8366249.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          267 a          115 c          129 g          257 t
ORIGIN      
        1 ttttggtatc tacttcattt tcggtcaact gagttaatga aatttattgt cgggcggttt
       61 tctagattca atttttctat atttggatag aagaatttac ttttttacct tttgacatat
      121 tttcttctta ttattttaat gatccctttt tcaaattaga aagatagcct ttgtatcgta
      181 ttagatgaaa tattcacaaa gctaaccatt atacttgcat ttttttcaaa atagagttat
      241 tacgtaaata gggataaata aaaaatactt attgaaagga tgatcaaaaa tggcaaatgt
      301 acctagcatt cgcgacatgt tccctgattt caatgacgtg tttagtccag cattcaatga
      361 ttttttaggt gtttcaagtt atccaaaagt agatttagtc gaaaatgaaa aagaatacaa
      421 actaactgct gatatgcctg gctgtgacaa agaagacacg acagttgaat attcagataa
      481 tactttgacg atctccgcca atcatgagtc ccatacagaa gataaagaag atggcaacta
      541 tgtacggaaa gaaagacatt cggtctctta caaacgttca ttctatcttc ctaatgttga
      601 tgaagaaaaa atcactggaa cctttaaaaa cggtgtgctg aaattggtat tacctaaaac
      661 agcttatcag ccaaaagaaa caaaaaaaat tgagttaaat tagaacttat cgcctctcga
      721 tgaatgaggg gcgattttta tatatttgga taagaacgag tatgttta
//
LOCUS       DAXWLB010000340          761 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXWLB010000340 DAXWLB010000000
VERSION     DAXWLB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.352"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            51..731
                     /locus_tag="ACGE4H_002929"
     CDS             51..731
                     /locus_tag="ACGE4H_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFF8366250.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
BASE COUNT          270 a          127 c          152 g          212 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
      661 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
      721 tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAXWLB010000341          753 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.353, whole genome shotgun sequence.
ACCESSION   DAXWLB010000341 DAXWLB010000000
VERSION     DAXWLB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.353"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            363..731
                     /locus_tag="ACGE4H_002930"
     CDS             363..731
                     /locus_tag="ACGE4H_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFF8366251.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          117 c          157 g          235 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt ttt
//
LOCUS       DAXWLB010000342          748 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXWLB010000342 DAXWLB010000000
VERSION     DAXWLB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.354"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            330..>748
                     /locus_tag="ACGE4H_002931"
     CDS             330..>748
                     /locus_tag="ACGE4H_002931"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8366252.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELE"
BASE COUNT          283 a           83 c          124 g          258 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagta
//
LOCUS       DAXWLB010000343          734 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXWLB010000343 DAXWLB010000000
VERSION     DAXWLB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.355"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>734
                     /locus_tag="ACGE4H_002932"
     CDS             <1..>734
                     /locus_tag="ACGE4H_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFF8366253.1"
                     /translation="TVIKLSSVLLIILFVSTKDDLWIYVFITSITNFLGSLVFWLSIH
                     GKVGKPLFNSNVFKQLWKPAFIILTPQLFMQVYTTLDKTIVGYFVDPTALSYYDQSQK
                     IARIILAVLTSITIVMLPKISKAQAEGKSENILVYTKKSFDYTIIIALILFSVVFINT
                     KEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQFAIAIEKDKEYAYPMIIGAI
                     VSIIGNVAFVPTYGAVAATIVLVVVE"
BASE COUNT          224 a          102 c          130 g          278 t
ORIGIN      
        1 tacagttatc aaattatcat cggtattgtt aataatcctt tttgtttcaa ctaaagatga
       61 tttgtggatt tatgtgttca ttacttcaat tactaacttt ttaggaagtt tagttttctg
      121 gttgagtatc cacggaaaag taggtaaacc actctttaac tctaatgttt ttaaacagtt
      181 gtggaaacca gcttttatta ttttgactcc tcagttgttt atgcaagtgt acacaacttt
      241 agacaaaacg atagttgggt attttgtgga tccaacagca ttatcttatt atgatcagtc
      301 tcaaaaaatt gctcgtatta tattggcagt cttaacatca attactattg ttatgttgcc
      361 taaaatatct aaagcacagg cagagggaaa atcagaaaat attttagttt acacaaaaaa
      421 atcttttgat tacacgatta ttattgcatt aattcttttt agtgtggtgt ttatcaatac
      481 aaaagaattt gttccatggt tttttgggga gaatttcaaa ccgatgacaa taaacatgct
      541 tatttcttgt ctaattatag ttgttagtcc aattggtgga gtttttgcta accaatttgc
      601 aattgctata gaaaaagata aggagtatgc ttatccaatg atcattggtg cgattgtaag
      661 tattattgga aatgtagctt ttgtgcctac ttatggcgct gtagcggcta caatcgtact
      721 cgtggtagta gaat
//
LOCUS       DAXWLB010000344          720 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.356, whole genome shotgun sequence.
ACCESSION   DAXWLB010000344 DAXWLB010000000
VERSION     DAXWLB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.356"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            97..>720
                     /locus_tag="ACGE4H_002933"
     CDS             97..>720
                     /locus_tag="ACGE4H_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366254.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPET"
BASE COUNT          236 a          113 c          160 g          211 t
ORIGIN      
        1 gatttatttt cttttctaat tcaacctgtg gtatgatgag agcataagta tcatgtgaaa
       61 tgatatgaag tatgtggaag atggacgggg aagaatatgg aagcaaaaat cactttagaa
      121 ccttttgaac gtatcttaag cggttaccgc aaagtagaag agttagctgt gaacgtaact
      181 gattgttcaa aattagcgca aaaatacgca agattcggcg ttgaaggcta tcgattagga
      241 aactatgtcg gtacagggta tctgaaccgt tatttagagt gtatggtgga ccgcgcacca
      301 atgctgattt atagacaaaa ttatctgatt ccgctgcttt ttcgtcgttc tgattcagcc
      361 tttcggttat ttgaagaaga gtatcggatg gaggctttct ttctactatt agagtggagc
      421 ttgaaacatc gtcctgaaaa aattcttatc gaaagaaatg agaagataga cacgaaaaaa
      481 aataaagtga tcgatagtgc atacctagca tttcgtgtat cagagatttt ggattgtggc
      541 ggctatccaa tcagcaattt ccaatcaatt gaccaattca ttgaatggaa tcgaatttac
      601 cgattgatcg acaatggcgg gattggccgt catagcaagg tatttgaccc agaatatcca
      661 gaaaacatgg aagaactgaa aatgattatt tcgcttgtca aattaaaata tcctgaaaca
//
LOCUS       DAXWLB010000345          717 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXWLB010000345 DAXWLB010000000
VERSION     DAXWLB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.357"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..96
                     /locus_tag="ACGE4H_002934"
     CDS             <1..96
                     /locus_tag="ACGE4H_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8366255.1"
                     /translation="PRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            97..>717
                     /gene="aac(6')-Ie"
                     /locus_tag="ACGE4H_002935"
     CDS             97..>717
                     /gene="aac(6')-Ie"
                     /locus_tag="ACGE4H_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFF8366256.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEI"
BASE COUNT          302 a           55 c          130 g          230 t
ORIGIN      
        1 cctagagctt gccatgtata tgaaaaatta ggtggtaaaa atgattataa agatgaaata
       61 gtatatgtat atgattatga aaaaggtgat aaataaatga atatagttga aaatgaaata
      121 tgtataagaa ctttaataga tgatgatttt cctttgatgt taaaatggtt aactgatgaa
      181 agagtattag aattttatga tggtagagat aaaaaatata cattagaatc attaaaaaaa
      241 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
      301 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
      361 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
      421 agtaaaggaa ttggtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga
      481 aatgctaatg cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac
      541 caaaaatctg gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa
      601 aaagaagatt gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca
      661 atgaaatatt taattgagca ttactttgat aatttcaaag tagatagtat tgaaata
//
LOCUS       DAXWLB010000346          696 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXWLB010000346 DAXWLB010000000
VERSION     DAXWLB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.358"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(54..629)
                     /locus_tag="ACGE4H_002936"
     CDS             complement(54..629)
                     /locus_tag="ACGE4H_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFF8366257.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAXWLB010000347          691 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXWLB010000347 DAXWLB010000000
VERSION     DAXWLB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.359"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            304..>691
                     /locus_tag="ACGE4H_002937"
     CDS             304..>691
                     /locus_tag="ACGE4H_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366258.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHY"
BASE COUNT          258 a           86 c          109 g          238 t
ORIGIN      
        1 tattatataa attaataata tactaccatt tgttggtagt ataatttaaa ggtaataagt
       61 tattctttta ttactatgtt tgggttttat agcaaagaaa gtttaataag agtttggacc
      121 tagcaaacaa tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat
      181 ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa tagatattta gggtagaaaa
      241 aaagaaagtc cattctgtaa aattaaagtt accacaaccg aaattttata ggaggacttt
      301 ctcatgccca attttactac agaaattatg gaaacactaa tcaataaagg tgatttagat
      361 gaattatttc gatgtcattt agaattagct gttaattcat tattgcaggc tgaattaaca
      421 gcttttcttg attatgaaaa atatgatcgt gctgggttca attcaggtaa ttcccgtaat
      481 gggaattatt cacgttcatt cagaacagag tatggagaat taaatttggt aattcctaga
      541 gatagaaatg gtaagttttc tcaacaaact ttacctgctt ataaaagaac caatgactct
      601 ttagaaacca ctattattca gctatttcaa aaaggaatta ctatgtctga aatctctgaa
      661 ttgattgaaa aaatgtacgg gcatcactat a
//
LOCUS       DAXWLB010000348          690 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXWLB010000348 DAXWLB010000000
VERSION     DAXWLB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.360"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            269..>690
                     /locus_tag="ACGE4H_002938"
     CDS             269..>690
                     /locus_tag="ACGE4H_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HFF8366259.1"
                     /translation="MRSSKYTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVL
                     KGIEIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFA
                     QLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQF"
BASE COUNT          228 a          118 c          111 g          233 t
ORIGIN      
        1 cgttaacttt attctacaga agcgacttcc ttttttgtat ggattttttc atttacacaa
       61 aatattttac actctctaat aattacacac acacaatttt ttaaggtctt aaaaccatca
      121 tatatgcaaa agaagctaac ctttattact aaattagacc cgtggtgcta gttaattttt
      181 tcctagagga aaatgtctaa acatgttggt aaatcaacaa aaaaacgcat atagtagtct
      241 caaaactccc aagaaatgag gactacatat gcgttcatca aaatatactg aacattttga
      301 cttagccaat cctgttacta aagtcgatga tatccccgat tatgaaatgt acagtcaaac
      361 aatcgatagc ttaaataagc gttttggcaa tcgtgtttta aaaggcatag aaatcggtta
      421 catcgcttct gaaaaggatc ggatcatcga ctatttagcc gataaagatt acgatttaaa
      481 attactcagt gtgcatcata atggtcaatt tgattattta gatgacgaag tcaaagatat
      541 ggatcctgct attgtgattc ctcagtattt tgcacaactt tcagaagcac tagttgttat
      601 agaagcagat gtctttgctc attttgacta tggttttcgg gtatttggtc tatccgttgc
      661 tgaatttaaa caatatgaag cgcagttttt
//
LOCUS       DAXWLB010000349          669 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXWLB010000349 DAXWLB010000000
VERSION     DAXWLB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.362"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(126..548)
                     /locus_tag="ACGE4H_002939"
     CDS             complement(126..548)
                     /locus_tag="ACGE4H_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366260.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT          221 a          110 c           93 g          245 t
ORIGIN      
        1 tgtgtctcct gtaaagttcg ctgaacttgt aaagctgatt cttctttcgg taaattaatt
       61 ttacttagaa tatcgctatc ttgataaaaa tttggtgtgt tcacaaattc tggcataaaa
      121 aatccctact ttctagttaa ggttcctgaa taagtcgcaa tccaattgtt tccagctgat
      181 ttataacttt gtaaatacaa ttttccatag taccaataac catttctttt ttctttccaa
      241 ttccgtgatt gaggaggtct agaaggccaa ccaactttat caaaaatcat cgttcctgtc
      301 actcgtatcg ttttagaagc tgctcgagca agtaatggag aaactattaa ataattttca
      361 gtaacagtta agtcgttagt tgggttacca atggctattc cgttcctatc taaaggagtt
      421 aattcttggt tattatctac tacagtagtt ggttcatttg tactatcagc aaatgtagga
      481 attgtagttc cagttacact aattccaata gttaaaatac ctaaagataa aataaactta
      541 tttttcattg tctatcacct cgaaatcaaa aataagaaaa cgttttctta ataaatctta
      601 acagataaat caagttatat actagaaaat tcataaaata tatttttaat gtcataatcg
      661 tttaaattt
//
LOCUS       DAXWLB010000350          664 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXWLB010000350 DAXWLB010000000
VERSION     DAXWLB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.363"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>664
                     /locus_tag="ACGE4H_002940"
     CDS             <1..>664
                     /locus_tag="ACGE4H_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFF8366261.1"
                     /translation="KIPEDEKINIYQLVTKKEKKLFLNTTFENLTMINPNIMNFEDES
                     IIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLITAILVKTTSPGPIIYKQVRI
                     TKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVGKFIRAVRFDELPQIFNVLK
                     GDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGYAQVYGKYVSDYNSKLRFDL
                     LYI"
BASE COUNT          235 a          117 c          113 g          199 t
ORIGIN      
        1 aaaatcccag aagatgaaaa aatcaacatt tatcaattag tgactaaaaa agagaagaaa
       61 ttatttttaa atacgacttt tgagaatctg acaatgataa atccgaatat tatgaatttt
      121 gaagatgaaa gcatcattga agcttctggg ttcagaatcc aaccagaata cgaactattc
      181 aagcgtctct ttgatatcat cgtttcgctt attttactga tcctcgcctc accttttatg
      241 ctaatcacag cgattttggt aaaaacaact tcacctggac caatcatcta taaacaagta
      301 cgtattacga aaaatcaaaa ggaattttca atctataaat tccgttccat gagtgcgaca
      361 gctgaagcaa aatcaggacc agtcttggct aaaagtaacg atgcacgtgt gacaccagta
      421 ggtaaattta tccgtgctgt acgttttgac gaattacctc aaatctttaa tgtattgaaa
      481 ggcgatatgt cgattgtcgg tccaaggcca gaaagaccat ttttcgttga tcagttcaat
      541 gaagaaaatc cttattatta cttaagacac aatgtacgtg cgggaattac cggatatgca
      601 caagtatacg gaaaatatgt atcagattat aacagtaaac tacgttttga tttgttgtat
      661 atca
//
LOCUS       DAXWLB010000351          662 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXWLB010000351 DAXWLB010000000
VERSION     DAXWLB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.364"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(153..452)
                     /locus_tag="ACGE4H_002941"
     CDS             complement(153..452)
                     /locus_tag="ACGE4H_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8366262.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          216 a          115 c           76 g          255 t
ORIGIN      
        1 tttgtgttaa ttaaaggata aaatacaata taaagaaaca tctgtttctt tatttagaat
       61 aaaaaaatac agactctgtt caaataaatg aacctagcct gcattatgag attgactata
      121 ctttttaatt cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc
      181 gaagccattc ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa
      241 aacggtgata ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc
      301 aaattattta tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc
      361 aaaagtatac gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt
      421 accatgtttt ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct
      481 tcccactaca ttctataaac aaagtatacg ctacatctag atttttttaa atcattactg
      541 gattgttaaa acctttcaat acactgttat ggcaacgatt tttattttaa aaacattcta
      601 aattaaatca gtaaaaattt ttattctaag atttttatta gaattacgcc taatttgtgt
      661 ct
//
LOCUS       DAXWLB010000352          657 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXWLB010000352 DAXWLB010000000
VERSION     DAXWLB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.366"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            73..234
                     /locus_tag="ACGE4H_002942"
     CDS             73..234
                     /locus_tag="ACGE4H_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFF8366263.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>657
                     /locus_tag="ACGE4H_002943"
     CDS             253..>657
                     /locus_tag="ACGE4H_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8366264.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGV"
BASE COUNT          271 a           62 c          114 g          210 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagta
//
LOCUS       DAXWLB010000353          651 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXWLB010000353 DAXWLB010000000
VERSION     DAXWLB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.367"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            21..620
                     /locus_tag="ACGE4H_002944"
     CDS             21..620
                     /locus_tag="ACGE4H_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8366265.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          247 a           96 c          148 g          160 t
ORIGIN      
        1 aattacggga ggctctacaa atgaacggtt ttgaaaagag agcacaagag aaaaagaaag
       61 agattttaca agcagctttt gacttgatga acacagacat aggaaccgct ggagtgacaa
      121 tggataaggt agctgagaac gctcaggtaa gtaaagcgac gatttttaaa tattttggca
      181 gcaaggaagg gctgatttat gaagtatttt ttgcatttat gaatgattta gggcaagaag
      241 cgaaagcgtt gattgcggaa aatcttccat ttgaagaaac actgattgcc atgagtgaaa
      301 acaaaatccg ttatctcaat cgagtgaaca aacaatttta tatcgatttg atgacatact
      361 atacaaaaaa agaaaaaaaa gaattttctg acgcgatgga tgcgtatacg caagaaagtt
      421 tcagtatcat gcttgatctt ttccatcgag ggcggaaaga aggaaaagtg gatttgaaat
      481 attcggatga gttcttgatc ctctattttc aagcaatggt agaaggaatt tctaagccgg
      541 agatatacgc aaaactgctt ccatatacag cagaatggac agaagtcctg atcaagggaa
      601 tcgcaccgaa gaagaaataa agcataaaaa agccgaaact gacgataaaa t
//
LOCUS       DAXWLB010000354          650 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXWLB010000354 DAXWLB010000000
VERSION     DAXWLB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.368"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..>650)
                     /locus_tag="ACGE4H_002945"
     CDS             complement(<1..>650)
                     /locus_tag="ACGE4H_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366266.1"
                     /translation="QFLEELSGEEYRLFLNNNILKLISYAKKANSLSLVDNLDNLLPD
                     KLIYDENEDNSQKQIIIFELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPF
                     FTLINFMLYVKEHLGYDDLVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYK
                     EQNIKTLELIKKNIKYYDPLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKE"
BASE COUNT          215 a           94 c           75 g          266 t
ORIGIN      
        1 gttctttata cattaaaatt tcttctttta actcatcaat tctaggagat ctaacatcag
       61 aatcagcaat tgcatttaat aacttatcaa tattttcttt attcgctagt aaaggatcat
      121 agtattttat attcttttta attaattcca gagtttttat attttgttct ttgtaattag
      181 ctagaatttc ttctacagtt tcgcctgaaa tcatagccat atctgaacca gtattgattc
      241 ctttgaatgg ataaggttct ttatcatatg cctttatcat ttctactaaa tcatcatatc
      301 ctaagtgttc tttaacatac agcataaaat ttattaatgt aaaaaatggc attgaagaat
      361 agtctggaaa tataaattga aacagtttgt tcgcaaaata tttatcatca tcattgcttt
      421 ctggtgacac tgacaggctg ctaagccact ttaaatacaa ttcaaatata attatttgtt
      481 tttgtgaatt atcttcattc tcatcataaa taagtttatc gggtaaaaga ttatctaagt
      541 tatctactaa agataggcta ttagctttct tggcataaga tattaatttt aaaatattat
      601 tatttaggaa taatctatat tcttctccag ataattcttc taaaaattgt
//
LOCUS       DAXWLB010000355          636 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXWLB010000355 DAXWLB010000000
VERSION     DAXWLB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.369"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            74..>636
                     /locus_tag="ACGE4H_002946"
     CDS             74..>636
                     /locus_tag="ACGE4H_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366267.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNIT"
BASE COUNT          234 a          106 c          119 g          177 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattac
//
LOCUS       DAXWLB010000356          627 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXWLB010000356 DAXWLB010000000
VERSION     DAXWLB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.370"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..322)
                     /locus_tag="ACGE4H_002947"
     CDS             complement(<1..322)
                     /locus_tag="ACGE4H_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8366268.1"
                     /translation="MSTNKKDNKKEEIIFTAMKHFSKFGYEKTKLLDIANEAKTSTTT
                     VYSFFKTKHDLYEAAVEYDLNIINFELERIVSESSTLDEYIINIIERVRLKSDKKQLL
                     LNFYM"
     gene            complement(327..>627)
                     /locus_tag="ACGE4H_002948"
     CDS             complement(327..>627)
                     /locus_tag="ACGE4H_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8366269.1"
                     /translation="PSDKKMITEWVKQFYNNRSFFDVYWSTVKELQNEKEISTKIDPV
                     IYTFIIDTMMLSGELLQNTQYQEIKAKVFLEDSIKSSLILKENVIETLTVLAKGQ"
BASE COUNT          200 a           93 c           68 g          266 t
ORIGIN      
        1 gcatataaaa atttaacaat aattgcttct tatcagattt tagccgtact ctttcaatta
       61 tatttataat atattcatcg agtgtggaac tttcactaac aattctttca agttcaaagt
      121 ttattatatt taaatcatac tctactgcag cctcatacaa atcatgtttt gttttaaaaa
      181 aactatatac agtagtagta ctagttttag cttcattagc aatatctaaa agctttgttt
      241 tttcataacc aaatttagaa aaatgtttca ttgcggtaaa tataatttct tcttttttat
      301 tatctttctt gtttgttgac ataaatttat tgcccctttg caagtacagt tagcgtctcg
      361 ataacatttt cttttaatat taatgaactt tttatagagt cttctagaaa aactttggct
      421 tttatttctt gatattgtgt attttgaagc agctctccag ataacatcat tgtatcaata
      481 ataaatgtat agataactgg atcaatttta gtactaattt ctttttcatt ttgtagttct
      541 ttaacagtac tccagtaaac gtcaaaaaaa cttctattat tatagaattg tttaacccat
      601 tcagttatca tttttttatc actagga
//
LOCUS       DAXWLB010000357          625 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXWLB010000357 DAXWLB010000000
VERSION     DAXWLB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.371"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>625
                     /locus_tag="ACGE4H_002949"
     CDS             <1..>625
                     /locus_tag="ACGE4H_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFF8366270.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYS"
BASE COUNT          183 a          134 c          140 g          168 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgcagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgattttc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagcc
//
LOCUS       DAXWLB010000358          612 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.372, whole genome shotgun sequence.
ACCESSION   DAXWLB010000358 DAXWLB010000000
VERSION     DAXWLB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.372"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..529)
                     /locus_tag="ACGE4H_002950"
     CDS             complement(<1..529)
                     /locus_tag="ACGE4H_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFF8366271.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRK"
BASE COUNT          206 a          130 c           97 g          179 t
ORIGIN      
        1 gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca taagaaatga
       61 taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg ctttttctag
      121 gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa gtctgtgtca
      181 caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg ctatttgctg
      241 gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc aaaattccga
      301 cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga ttatctaaac
      361 gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg
      421 gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc agagtgatat
      481 aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt tgtaaaactc
      541 ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt
      601 atacatacaa aa
//
LOCUS       DAXWLB010000359          597 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXWLB010000359 DAXWLB010000000
VERSION     DAXWLB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.373"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>597
                     /locus_tag="ACGE4H_002951"
     CDS             <1..>597
                     /locus_tag="ACGE4H_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFF8366272.1"
                     /translation="SDSFLAAFYLYTAKYWIDDSRKTIKYAKRKKQFYFQTWHGTPLK
                     KIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEKYVSAFNIVPSKILEVGTPRN
                     DELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQELN
                     LMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEM"
BASE COUNT          229 a           82 c           97 g          189 t
ORIGIN      
        1 tccgattctt ttttagctgc cttttatttg tatacagcta agtattggat agacgatagt
       61 cggaaaacga ttaagtatgc aaaaaggaaa aaacaatttt attttcaaac atggcacggt
      121 acgcctttaa aaaaaattga atttgatgca aaagataagt tacctaaacg ttacctaagc
      181 tatgcgaaaa aagatagtga gtcaattaca tatttattat ctggtaatcg ctatagttct
      241 gagaagtatg tttcagcatt caatatcgtt ccttctaaaa tattagaagt aggaactcct
      301 agaaacgatg aattagcctc tagtaaagag caagtgtttg acttgaaaaa gaaacaaatc
      361 aatgtcttat ttgctccaac ttttcgaaat aatattgagg acaacggaat tacgcaacta
      421 gaatggttag gtgtggataa cttacgtgaa ttttttaaaa agcaacaaca agaattaaat
      481 ttaatgacta aatttcatcc taatgttcat agtaaattag caactgattc taaaagtata
      541 gaatatatga aaaaacatca aattacttta attaatgatg ggattcctct tgaaatg
//
LOCUS       DAXWLB010000360          568 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXWLB010000360 DAXWLB010000000
VERSION     DAXWLB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.374"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>568
                     /locus_tag="ACGE4H_002952"
     CDS             <1..>568
                     /locus_tag="ACGE4H_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFF8366273.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DAXWLB010000361          563 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXWLB010000361 DAXWLB010000000
VERSION     DAXWLB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.375"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..515
                     /locus_tag="ACGE4H_002953"
     CDS             <1..515
                     /locus_tag="ACGE4H_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFF8366274.1"
                     /translation="PKYRTNLGMVFQSFNLFNNMNVLENCMSGQTTVLKRDKETAKKV
                     AIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPDVLLFDEPTSALDPEMVGE
                     VLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVIAEEGTPEDIFVHPKEERT
                     KEFLSRILNA"
BASE COUNT          185 a          104 c          122 g          152 t
ORIGIN      
        1 tacctaaata tcgaacgaat ctaggtatgg ttttccaatc cttcaacctg ttcaacaata
       61 tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt cctgaaacga gataaagaaa
      121 ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg tatggatcgt tatatcgaag
      181 ccaaaccagc acaattatct ggtggacaga aacagcgtgt cgcaattgct cgggcattat
      241 caatgaatcc agatgtattg ctattcgacg aacctacttc ggcgttagac cctgaaatgg
      301 ttggcgaagt gcttaatacg atcaaagact tagcacatac tggcttgaca atgcttatcg
      361 tgacgcatga gatggaattt gcaaaagatg tatctgaccg tgtcattttt atggataaag
      421 gtgtgattgc agaagaaggt acaccagaag atatctttgt ccatccgaaa gaagaacgta
      481 caaaagaatt cctttcacgt attttaaatg cttaggatca atagctgatt gggacaaaga
      541 gctgcggtgt ttaccgcagt tct
//
LOCUS       DAXWLB010000362          547 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXWLB010000362 DAXWLB010000000
VERSION     DAXWLB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.376"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..378)
                     /locus_tag="ACGE4H_002954"
     CDS             complement(<1..378)
                     /locus_tag="ACGE4H_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366275.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISY"
BASE COUNT          218 a           78 c           78 g          173 t
ORIGIN      
        1 ataggaaata aaagaaaatg ataggcattg tatacctaat aaagtaaaaa tcttaggtgg
       61 cagataaaaa cacaaaaata gcaagagaat gtaatagcgt ttcttccact ttttgttatc
      121 agaccttata gcgatcagag gaataccaaa tgcataactg aaaaactctt gatgtactgt
      181 taatatgtca cggccttgaa taataaatgc gacaatcccc gctataatta gtgtgcctaa
      241 caccaaaacg gaaaattgat gaatatattt tatatacaaa tgatccattg tacttcctgc
      301 ctttttattt gtgagaaaaa caaatccgta tacgtgaata acttctagaa ataaaagaaa
      361 tttgcaaaac cactccattt atctacttct acgtgcattt tttgatattg ttttctttat
      421 ttaagattat acagaaaaat atctagggtc gctggattct aagtaaaaaa aatttttaaa
      481 ataaaaagaa ctaaatcaag tagtaattaa actactgttg atttagttct aataaaaaga
      541 tttagat
//
LOCUS       DAXWLB010000363          536 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXWLB010000363 DAXWLB010000000
VERSION     DAXWLB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.377"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..236
                     /locus_tag="ACGE4H_002955"
     CDS             <1..236
                     /locus_tag="ACGE4H_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8366276.1"
                     /translation="SLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            246..>536
                     /locus_tag="ACGE4H_002956"
     CDS             246..>536
                     /locus_tag="ACGE4H_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFF8366277.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSP"
BASE COUNT          219 a           54 c           81 g          182 t
ORIGIN      
        1 cttccttatt tttaggaaat gtattactaa aagtaattgt agatggattc attaactcag
       61 aggattcaat gatttccggt ggaagtttaa taaataatag tagttttatg tcaaatataa
      121 caacacttgg acaaagttat ttaatattaa taagtattat tgttttatca gttgtatttg
      181 cttcttcatt aatgttaatc aagaagccta aagaaatctt atcaaaaata agttaggaga
      241 aaataatgga tatattagaa ataaagaatg tagcatatag ttatgcaaat tctaaagaaa
      301 aagttttgtc aggagtaaat caaaaatttg aacttgggaa gttttatgcg atagtaggaa
      361 agtcaggaac aggaaaatct acacttcttt ccttacttgc tggacttgat aaacctcaaa
      421 caggaaaaat attgtttaag aatgaagata tacaaaataa aggatatagt aatcatagaa
      481 aaaataatat atctttagta tttcaaaact ataatttaat agattattta tcacca
//
LOCUS       DAXWLB010000364          525 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.378, whole genome shotgun sequence.
ACCESSION   DAXWLB010000364 DAXWLB010000000
VERSION     DAXWLB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.378"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>525
                     /locus_tag="ACGE4H_002957"
     CDS             <1..>525
                     /locus_tag="ACGE4H_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFF8366278.1"
                     /translation="LGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDF
                     SENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVE
                     KDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGT
                     SKIQIIMQFIFELI"
BASE COUNT          207 a           43 c           93 g          182 t
ORIGIN      
        1 aattaggtga tgaagttgat cttgaattac tagatattga aaaaagtgga aaaataaaaa
       61 gtcataaatt taaaattata gggatctttt ctggtaaaaa acaggaaaca tatacaggat
      121 tatcttctga ttttagtgaa aatatggtgt ttgtagatta ttcaacaagc caagagatat
      181 taaataattc agaaaataat gaaattgcaa ataaaatttt aatgtattct ggtagtgcag
      241 aatctacaga cttagcctta aacaaattga aagagcttaa aattgatgag tcaaagtatt
      301 ttgttgaaaa agattctaat gcgtttgaag agtctttaga gtcagtgagt ggaataaaat
      361 atataataaa agtaatgact tattctatta tgttaggtgg gatggttgtt ctttcattaa
      421 tcttgattct atggttaagg gaaagaattt atgaaatagg tatattctta tctattggaa
      481 catctaagat acaaattata atgcaattta tattcgagtt aatat
//
LOCUS       DAXWLB010000365          522 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.379, whole genome shotgun sequence.
ACCESSION   DAXWLB010000365 DAXWLB010000000
VERSION     DAXWLB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.379"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            164..>522
                     /locus_tag="ACGE4H_002958"
     CDS             164..>522
                     /locus_tag="ACGE4H_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFF8366279.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLI"
BASE COUNT          176 a           63 c           86 g          197 t
ORIGIN      
        1 taccaattat aagcgtacaa gatagttatg gccttgttga tgtttttaaa aaccaacaag
       61 aaaagctttt gaaaagttct caagagttac ttgattatat tggaaattat gaaacagggg
      121 caagtacaga aaaggttctt gagttgttag agagggatat aaagtgaaaa aaacagtaaa
      181 agatatattt tataatgcga tatatcaagt ctttttgatt gttttgccct tacttactat
      241 acctatactt agtcgtagaa taggttcaac tggtttaggg atttatgggt atgttttctc
      301 aatatcacaa tttttaatga ctgtcattgc tgtgggaatg aatccttttc gtattcgaaa
      361 tatcgctaaa tcaaggaaag ataaaaaagc actatcttta caattctgga atatttactt
      421 tcttcaattt ttaataggct tgagtgtttc ctttctctat atagcctata tttatttttt
      481 tgatgtaaag tataaaaatt tatacttaat tcaattgata tt
//
LOCUS       DAXWLB010000366          516 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.380, whole genome shotgun sequence.
ACCESSION   DAXWLB010000366 DAXWLB010000000
VERSION     DAXWLB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.380"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            167..364
                     /locus_tag="ACGE4H_002959"
     CDS             167..364
                     /locus_tag="ACGE4H_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFF8366280.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            366..>516
                     /locus_tag="ACGE4H_002960"
     CDS             366..>516
                     /locus_tag="ACGE4H_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFF8366281.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPL"
BASE COUNT          201 a           52 c           89 g          174 t
ORIGIN      
        1 tgcattctca aaaaatatta aagaatatac atagtttata tacttaaaag tgattaaatt
       61 cttattcagg aattattttg ctacgtttca agaaggaatt gttttttgaa atttaatgaa
      121 tgttagaata atagtctaaa aacattaatt taggggtgat tagattatga aacatttaaa
      181 aattttgtct attaaagaga cacaacttat ctatgggggt accactcata gtggaaaata
      241 ttatggaaat ggagtgtatt gcactaaaaa taaatgtacg gtcgattggg ccaaggcaac
      301 tacttgtatt gcaggaatgt ctataggtgg ttttttaggt ggagcaattc cagggaagtg
      361 ctaaaatgaa aaaaaatgct aagcaaattg ttcatgaatt atataatgat atatctataa
      421 gtaaagatcc taaatattct gatattcttg aggttttaca aaaggtatat ttaaaattag
      481 aaaaacaaaa atatgaatta gatcccagtc ctttaa
//
LOCUS       DAXWLB010000367          515 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.381, whole genome shotgun sequence.
ACCESSION   DAXWLB010000367 DAXWLB010000000
VERSION     DAXWLB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.381"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT          230 a           71 c           83 g          131 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaa
//
LOCUS       DAXWLB010000368          509 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.383, whole genome shotgun sequence.
ACCESSION   DAXWLB010000368 DAXWLB010000000
VERSION     DAXWLB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.383"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            23..>509
                     /locus_tag="ACGE4H_002961"
     CDS             23..>509
                     /locus_tag="ACGE4H_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFF8366282.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FM"
BASE COUNT          186 a           92 c           85 g          146 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgg
//
LOCUS       DAXWLB010000369          508 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.384, whole genome shotgun sequence.
ACCESSION   DAXWLB010000369 DAXWLB010000000
VERSION     DAXWLB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.384"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>508
                     /locus_tag="ACGE4H_002962"
     CDS             <1..>508
                     /locus_tag="ACGE4H_002962"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366283.1"
                     /translation="EGIRSKNRIFRRIKRKLFGKNLRECINEGLIFGKKHSLVEVPEF
                     GSEPYLIEIGDNFRCSFGVKFITHDGGVWVLRKMSSVSESTSKIGRIKLGNNIFIGIY
                     SIVFPGVSIGDNVIIRANTLVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRT
                     SMILKQKN"
BASE COUNT          188 a           49 c          111 g          160 t
ORIGIN      
        1 gtgagggtat acgaagtaag aatagaatct ttcgaagaat aaagagaaaa ctatttggaa
       61 aaaatctgcg agaatgtatt aatgaaggtt tgatattcgg aaaaaaacat agtttagtag
      121 aggttcctga atttgggagc gagccatatt taattgaaat aggggataat ttcagatgtt
      181 catttggtgt taaatttata acacacgatg gtggagtctg ggtcttaagg aagatgagtt
      241 ctgtgagcga gtcaacttca aaaataggta ggatcaaatt gggaaataat atttttattg
      301 gtatttactc tattgttttc ccaggagttt caattggcga taatgtaatt attagagcga
      361 acactttagt aaataaagat atcccaaata atagtgttgt agcgggtagt tcatgtaaag
      421 tgatttcaac tatagaggag cattatgaga aaaataaaaa tgaatttatt aaatatcgaa
      481 cctcgatgat tttgaagcaa aaaaattt
//
LOCUS       DAXWLB010000370          495 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.385, whole genome shotgun sequence.
ACCESSION   DAXWLB010000370 DAXWLB010000000
VERSION     DAXWLB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.385"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            182..>495
                     /locus_tag="ACGE4H_002963"
     CDS             182..>495
                     /locus_tag="ACGE4H_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFF8366284.1"
                     /translation="MYKAANDLFNRLKLEKKELILKYILDEFGRVGYEATSVRKIASK
                     ADLSVGSLYQYFNNKEGMLTVAIEHVFVILDEYVSTINDAPLKLKDRLEIMFEMILKL
                     GK"
BASE COUNT          186 a           52 c           84 g          173 t
ORIGIN      
        1 tcgtttcaaa tcaaaacaaa aagagactca cttttggtaa aatttaagat gttaatcatt
       61 ttgtgtgtcg gttaattttt atcaatctaa gatttaatta tagatcatta aaaatgagcg
      121 gtcgttttta tatactgaat atggtacact aaattaaaat accttttttg aggagatcat
      181 tatgtataaa gcagcgaacg atttatttaa tcgattaaaa ttagaaaaaa aggaactcat
      241 tttaaagtat attttagatg agtttgggag agtaggttat gaagccactt ctgttcgtaa
      301 aattgccagt aaagcagatt taagtgtagg atcattatat cagtatttca acaataaaga
      361 aggtatgtta acagtcgcga tagaacacgt ttttgttatt ttagatgaat atgttagtac
      421 tataaacgac gctcctttaa aactaaaaga tcgtttagaa ataatgtttg aaatgatttt
      481 aaaattagga aagca
//
LOCUS       DAXWLB010000371          477 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.386, whole genome shotgun sequence.
ACCESSION   DAXWLB010000371 DAXWLB010000000
VERSION     DAXWLB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.386"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            22..>477
                     /locus_tag="ACGE4H_002964"
     CDS             22..>477
                     /locus_tag="ACGE4H_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFF8366285.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT          167 a           69 c          109 g          132 t
ORIGIN      
        1 taaattgttg gggttgagta aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa
       61 cagcagctac atgaagcggg gtttaagctg acaccgcaaa gagaagctac tctgcttgtg
      121 cttttggaaa atgagaaaga gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa
      181 aagagtccgg aaattggact tgctactgtt tatcgaaccc ttgaaatctt gacagaattg
      241 aaaattgtcg ataaagtaag ttttaatgat ggattagccc gttatgattt gagaaaagaa
      301 ggcgccaaac atttccatca tcatttacta tgcttgaact gcggcacgat cgaggaagta
      361 gaagaggatc ttttacttga agtggagcaa gtagtagaag aacgttatca ttttttagta
      421 aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat gctatgaaaa agaaaaa
//
LOCUS       DAXWLB010000372          464 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.387, whole genome shotgun sequence.
ACCESSION   DAXWLB010000372 DAXWLB010000000
VERSION     DAXWLB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.387"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>464
                     /locus_tag="ACGE4H_002965"
     rRNA            <1..>464
                     /locus_tag="ACGE4H_002965"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          141 a          103 c          131 g           89 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagt
//
LOCUS       DAXWLB010000373          462 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.388, whole genome shotgun sequence.
ACCESSION   DAXWLB010000373 DAXWLB010000000
VERSION     DAXWLB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.388"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..430)
                     /locus_tag="ACGE4H_002966"
     CDS             complement(<1..430)
                     /locus_tag="ACGE4H_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFF8366286.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAXWLB010000374          460 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.389, whole genome shotgun sequence.
ACCESSION   DAXWLB010000374 DAXWLB010000000
VERSION     DAXWLB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.389"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(20..91)
                     /locus_tag="ACGE4H_002967"
     tRNA            complement(20..91)
                     /locus_tag="ACGE4H_002967"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACGE4H_002968"
     tRNA            complement(113..200)
                     /locus_tag="ACGE4H_002968"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACGE4H_002969"
     tRNA            complement(225..298)
                     /locus_tag="ACGE4H_002969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACGE4H_002970"
     tRNA            complement(312..382)
                     /locus_tag="ACGE4H_002970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACGE4H_002971"
     tRNA            complement(384..459)
                     /locus_tag="ACGE4H_002971"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAXWLB010000375          459 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.390, whole genome shotgun sequence.
ACCESSION   DAXWLB010000375 DAXWLB010000000
VERSION     DAXWLB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.390"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT          162 a           52 c           65 g          180 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatattttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgtttta
//
LOCUS       DAXWLB010000376          445 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.391, whole genome shotgun sequence.
ACCESSION   DAXWLB010000376 DAXWLB010000000
VERSION     DAXWLB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.391"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            105..>445
                     /locus_tag="ACGE4H_002972"
     CDS             105..>445
                     /locus_tag="ACGE4H_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFF8366287.1"
                     /translation="MYRKSQMIARMSLMLALLIITARLVIPLPLFDYLSLQIITVYLI
                     YPTLGMKNGLIVTCSYLILGIIGLPIFASGGGYAYIFKPTFGFLLAFVTLPIIQSFIS
                     NKFNNAIYSSF"
BASE COUNT          140 a           66 c           66 g          173 t
ORIGIN      
        1 tacttgtatt cctcagattg gtaaaattcc ttatctaaaa aatttaaagt atgttttgaa
       61 atcagaaaaa gaaaaacaaa atttaattag ggagtggcat ttaaatgtat agaaagagtc
      121 aaatgattgc gcgaatgtca ttaatgttgg cgctgttaat tattacagct agacttgtta
      181 ttcctttacc tttatttgat tatttatctt tacagattat tactgtttat ctcatttatc
      241 ctacattagg tatgaaaaac ggtctgatcg taacctgtag ctacttaatt ctaggaataa
      301 taggtcttcc gatatttgca agcggtggtg gatatgcata tattttcaaa ccaacttttg
      361 gtttcttgct agcctttgtg actttaccta ttatacaatc gttcatctca aataaattta
      421 ataatgctat ctactcttct tttaa
//
LOCUS       DAXWLB010000377          444 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.392, whole genome shotgun sequence.
ACCESSION   DAXWLB010000377 DAXWLB010000000
VERSION     DAXWLB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.392"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>444
                     /locus_tag="ACGE4H_002973"
     CDS             <1..>444
                     /locus_tag="ACGE4H_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFF8366288.1"
                     /translation="IIIENEFGEVGIDHKLLVHREERVLQLNNGCMCCSLRSDLLAIL
                     ISLLETIEEHQQPISQIIIETTGIADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSF
                     ELIEREPQALKQLVMADRIFVSRKQNLNLSALKKLQREIKSINPL"
BASE COUNT          164 a           69 c           76 g          135 t
ORIGIN      
        1 ttattataat tgaaaatgag tttggtgaag ttgggattga tcacaaatta ttagttcata
       61 gagaggaacg ggtgttacaa ctaaataatg gatgtatgtg ttgcagtctt cgcagtgatt
      121 tattagcaat attgatttct ttactggaaa caatagaaga acatcaacaa ccaatttcac
      181 aaatcatcat tgaaactaca ggtatagcag atccccaacc aatcattcaa acacttttaa
      241 cagcgcctca actaagagga agagtatata tcgatagttt attgactgtt attgatagtg
      301 acagttttga acttattgaa agagaaccac aagcccttaa gcaacttgtc atggcagatc
      361 gcatttttgt gtctagaaaa caaaacttga atttatctgc actaaaaaaa ttacagagag
      421 aaatcaaaag tattaaccct ttat
//
LOCUS       DAXWLB010000378          441 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.394, whole genome shotgun sequence.
ACCESSION   DAXWLB010000378 DAXWLB010000000
VERSION     DAXWLB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.394"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT          180 a           48 c           74 g          139 t
ORIGIN      
        1 ctgtaatatt cgtgtttaaa aataataaat aaaaataggg aattttctat tgcaatttta
       61 taaagagatt gctatattat tatttgttct aaatgattcc tttaaggtgt cactaaaaat
      121 tataaaaaat agtgttgacg cacttttgat gtcatgttat aataacgaag tcgtttggat
      181 gagatagcta ataaagcttg attttatcga aaaaaacttc gaaaaagtta ttgacaaata
      241 acaataaaga tggtatgata atcaagtcgc tgctaatgag tggcaaagac gtttagaaaa
      301 aataaacaaa gaagttgttg acattctctt ctgaatctga taagattaaa gagttgttaa
      361 aacaaaaaga ttagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
      421 tcttgattca attcaagaca a
//
LOCUS       DAXWLB010000379          434 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.395, whole genome shotgun sequence.
ACCESSION   DAXWLB010000379 DAXWLB010000000
VERSION     DAXWLB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.395"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4H_002974"
     CDS             complement(<1..>434)
                     /gene="scm"
                     /locus_tag="ACGE4H_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFF8366289.1"
                     /translation="VNIDAEVLTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYN
                     DRSNHETEDFSDANISWNFGNEMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNV
                     ILDAATNRWDPNEIFISDNGTDTTDTSDSTDTSTTSDSSDSS"
BASE COUNT          118 a           77 c           86 g          153 t
ORIGIN      
        1 tgatgaatct gagctatcac ttgtggtaga tgtatctgta ctgtcgcttg tgtctgtcgt
       61 atctgtacca ttgtcgctga taaatatttc gttaggatcc caacgattgg tagctgcatc
      121 taaaatcaca tttgttccta taatcgttcc gtcttggttt tgagtaatgc ggatagtagc
      181 attaggcgca aggattgttc cttgaaaagg agcactgatt gtcaaatcag tcatctcatt
      241 tccaaaattc caagagatat tcgcatccga aaaatcttct gtttcatgat ttgaacgatc
      301 attgtatttg attggtgact gtacgttcaa tgcagaagaa ctgttgatga cattaaatac
      361 aactacttga tcatttggat taatgatttg aagtggcgta ttcatcgtta acacttctgc
      421 atcgatattt acca
//
LOCUS       DAXWLB010000380          429 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.396, whole genome shotgun sequence.
ACCESSION   DAXWLB010000380 DAXWLB010000000
VERSION     DAXWLB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.396"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>429
                     /locus_tag="ACGE4H_002975"
     CDS             <1..>429
                     /locus_tag="ACGE4H_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFF8366290.1"
                     /translation="SSSEDTSINSSTSSDQIDHLQDLENKRKELEEALEAAKNKLKED
                     QAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKEFNQQLNQVA
                     QTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKA"
BASE COUNT          180 a           83 c           84 g           82 t
ORIGIN      
        1 ttcatcttca gaagatacat caataaatag ttctacctct tctgaccaaa tcgatcattt
       61 gcaagacttg gaaaataaaa gaaaagaact cgaagaagca ttagaagctg caaaaaacaa
      121 attgaaagaa gaccaagcag caatcgatca agatttagct gatcttaaaa ataaagtggg
      181 aaatgaccgt gcacaacttg agaaaaagct tgccgaagcg aaagcaaaag cggaagctgt
      241 ccaaacagat cttgacggga agatccaaca agcaaaagaa ttcaatcagc aattaaatca
      301 ggttgctcaa acagcttctg gaaagctaag tcaaactcaa gcggatatca cagcggccag
      361 tcaagcaatc cacggaaaga tcagcgaagc acaaacagtt gctaatgatt ttgaagcaaa
      421 aaaagctga
//
LOCUS       DAXWLB010000381          424 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.397, whole genome shotgun sequence.
ACCESSION   DAXWLB010000381 DAXWLB010000000
VERSION     DAXWLB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.397"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(170..>424)
                     /locus_tag="ACGE4H_002976"
     CDS             complement(170..>424)
                     /locus_tag="ACGE4H_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFF8366291.1"
                     /translation="SDNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKD
                     YEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          130 a           83 c           58 g          153 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cact
//
LOCUS       DAXWLB010000382          418 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.398, whole genome shotgun sequence.
ACCESSION   DAXWLB010000382 DAXWLB010000000
VERSION     DAXWLB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.398"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..320
                     /locus_tag="ACGE4H_002977"
     CDS             <1..320
                     /locus_tag="ACGE4H_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366292.1"
                     /translation="ALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYS
                     TNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLE
                     SMI"
BASE COUNT          154 a           76 c           58 g          130 t
ORIGIN      
        1 aggcattgga tcacatcgac tttatgacta ggaaatggaa aaagcagtat ccaagagtcg
       61 tcaatttact cttgaatcct gccctattaa ccttttataa tttccctcac gccatcagac
      121 gaacaattta ttcgacgaac ctgattgaag gctttaataa gcagctaaaa cgatatactc
      181 gaagaaaaga acaattccct aatgaagaat ctctagagag attcttcatt tctcaattta
      241 atcaatataa ccaaaaattt ttaggtagaa ttcacaaggg atttaaagaa attcaggata
      301 cattagagtc gatgatttaa ctgtaattaa cagaatggat tttccattta catataattc
      361 ttgacgctac cataatctaa ataaccatca tattcatcta ctacttatat aattgagt
//
LOCUS       DAXWLB010000383          417 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.399, whole genome shotgun sequence.
ACCESSION   DAXWLB010000383 DAXWLB010000000
VERSION     DAXWLB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.399"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..402
                     /locus_tag="ACGE4H_002978"
     CDS             <1..402
                     /locus_tag="ACGE4H_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFF8366293.1"
                     /translation="AFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPILIDQ
                     ASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPY
                     NKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          168 a           55 c           53 g          141 t
ORIGIN      
        1 gcgtttatcg aatctattga ttctacaatc gatagattaa aaaaagaaat tcgattttat
       61 aatagagtaa ttggtatcgg aaatattata aaaatagtct tatcagctag tatcccaata
      121 ttaattgatc aggcttccga acacaggtct ttactattga ttgtttcaat tgcttcagca
      181 ataattacaa ttatacaaag tgggatgtct gcatttaatt atcaagataa agtacagact
      241 tcgacagagt tattaatgaa aattgaaaaa gaaaagttgt tatatataac gaaaacatcc
      301 ccatataata aaacagatga agaaaatttc catttaatcg ttacaaccct tcaaactgaa
      361 ttaaatgata ttatctctga ttttaatcaa gttaataact aaaaatttat ttaatta
//
LOCUS       DAXWLB010000384          415 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.400, whole genome shotgun sequence.
ACCESSION   DAXWLB010000384 DAXWLB010000000
VERSION     DAXWLB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.400"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..339)
                     /locus_tag="ACGE4H_002979"
     CDS             complement(<1..339)
                     /locus_tag="ACGE4H_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFF8366294.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXWLB010000385          412 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.401, whole genome shotgun sequence.
ACCESSION   DAXWLB010000385 DAXWLB010000000
VERSION     DAXWLB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.401"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..316
                     /locus_tag="ACGE4H_002980"
     CDS             <1..316
                     /locus_tag="ACGE4H_002980"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFF8366295.1"
                     /translation="NIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNL
                     KKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERIL
                     TI"
BASE COUNT          156 a           55 c           75 g          126 t
ORIGIN      
        1 taatatattt aaccatttga aaaagatgct aaatgtgtct gttactgaat cggcacctgt
       61 aaatattgaa atcgtacctc aaaatgtatc taaaggtgtg gtgtttgatc gtttggaatc
      121 aatctacaat ttaaagaaac ctttgagaat cgcgatcgga gatagtttaa atgacttatc
      181 tatgtttgaa agtgcagata ttagttttgc aatgggaaat agtcatcaag aaatcatgaa
      241 aaaagctgat tatgtcactg tttcaaatga taaagacgga gtatctcaag catttgaaag
      301 aattcttacc atataaacta attaaggtag tatagaacat agaattctaa atacggaaaa
      361 atggagcata aattccagta caaatagtac ttcttcagtg tcaaacgaag cg
//
LOCUS       DAXWLB010000386          412 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.402, whole genome shotgun sequence.
ACCESSION   DAXWLB010000386 DAXWLB010000000
VERSION     DAXWLB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.402"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT          145 a           67 c           71 g          129 t
ORIGIN      
        1 tttggttaaa acaagtacta gagttgaaaa atgtcttttt gaaccatgat ttagtgaatc
       61 atgggatgaa tttgtgaaaa aatgatccta agtagataca cggacgcaaa tttctatcgc
      121 tacatacaaa agcggatgag cttgtagagc aaatttataa tcagttagat gtaatttcag
      181 aacaattgat tatgatcgat gggttcctgt ataaactcta taggaaacac agttgaattg
      241 gataaataga ttcagaattt caaagcacct ggttatcttg attattctaa tatacattta
      301 aaggggcaac tccttcccct gagctacccc tagagagtga tataggaata ttcccaagtc
      361 tattataaga atacctacac gttatctcaa caaatttgct cttacactca ta
//
LOCUS       DAXWLB010000387          401 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.403, whole genome shotgun sequence.
ACCESSION   DAXWLB010000387 DAXWLB010000000
VERSION     DAXWLB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.403"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..380
                     /locus_tag="ACGE4H_002981"
     CDS             <1..380
                     /locus_tag="ACGE4H_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366296.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          153 a           72 c           63 g          113 t
ORIGIN      
        1 aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata
       61 ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac
      121 tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca
      181 tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa
      241 aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat
      301 acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc gatacattag
      361 tttcaatgtt tactgagtaa ctaattattt tgcaggagga c
//
LOCUS       DAXWLB010000388          394 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.404, whole genome shotgun sequence.
ACCESSION   DAXWLB010000388 DAXWLB010000000
VERSION     DAXWLB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.404"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(195..>394)
                     /locus_tag="ACGE4H_002982"
     CDS             complement(195..>394)
                     /locus_tag="ACGE4H_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366297.1"
                     /translation="SLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYL
                     VPQILGSLWAVALSYKKARHP"
BASE COUNT          151 a           48 c           78 g          117 t
ORIGIN      
        1 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
       61 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      121 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      181 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      241 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      301 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      361 acagtagata taatattggc aatgaataaa gata
//
LOCUS       DAXWLB010000389          389 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.405, whole genome shotgun sequence.
ACCESSION   DAXWLB010000389 DAXWLB010000000
VERSION     DAXWLB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.405"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            4..>389
                     /locus_tag="ACGE4H_002983"
     CDS             4..>389
                     /locus_tag="ACGE4H_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFF8366298.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANIN"
BASE COUNT          140 a           47 c           71 g          131 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaatta
//
LOCUS       DAXWLB010000390          382 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.406, whole genome shotgun sequence.
ACCESSION   DAXWLB010000390 DAXWLB010000000
VERSION     DAXWLB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.406"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            138..>382
                     /locus_tag="ACGE4H_002984"
     CDS             138..>382
                     /locus_tag="ACGE4H_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFF8366299.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
BASE COUNT          132 a           43 c           72 g          135 t
ORIGIN      
        1 tatcttttat tttaaaatat gggatattat gataaattaa ctatggatga tatttgaaaa
       61 aaacatactg tatatttgat ttaattttta tttttaagct catatcgtaa gaatgggact
      121 tgtagtctca cttctttatg gaggtgataa ttatgaacgt tggttttatt ggagccggca
      181 aggtaggttg ttcattcggg aagtatttcc aggaacataa aatacaagta acaggatttt
      241 atagtaaaag cgaagactcg tcattagcag cttcgaattt tacatctagt aaacaatatt
      301 taaacttaag agaattagta gatgaaaatg atactatttt cattacgact cctgatggtc
      361 aaattcagga ggtatggcaa ga
//
LOCUS       DAXWLB010000391          366 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.408, whole genome shotgun sequence.
ACCESSION   DAXWLB010000391 DAXWLB010000000
VERSION     DAXWLB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.408"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..188)
                     /locus_tag="ACGE4H_002985"
     CDS             complement(<1..188)
                     /locus_tag="ACGE4H_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366300.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT          104 a           81 c           58 g          123 t
ORIGIN      
        1 aatggataat cttctgtcgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgctacttt gataagcttt tgccgctttg tctgtgacga caaaagatct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 tgaaccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcaatga gttggcggaa aacaattcat tgaaaaaaag agtcctggac aatcacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacac tctgtgagtg aaatggactc
      361 ttcaat
//
LOCUS       DAXWLB010000392          352 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.409, whole genome shotgun sequence.
ACCESSION   DAXWLB010000392 DAXWLB010000000
VERSION     DAXWLB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.409"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..>352)
                     /locus_tag="ACGE4H_002986"
     CDS             complement(<1..>352)
                     /locus_tag="ACGE4H_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366301.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAE"
BASE COUNT          132 a           58 c           42 g          120 t
ORIGIN      
        1 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
       61 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
      121 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
      181 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
      241 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
      301 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAXWLB010000393          344 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.410, whole genome shotgun sequence.
ACCESSION   DAXWLB010000393 DAXWLB010000000
VERSION     DAXWLB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.410"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>344
                     /locus_tag="ACGE4H_002987"
     CDS             <1..>344
                     /locus_tag="ACGE4H_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFF8366302.1"
                     /translation="NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKL
                     LNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLP
                     NILGDKGYISHK"
BASE COUNT          126 a           62 c           55 g          101 t
ORIGIN      
        1 aaacttagct tacactatca agattattcg atactttttc atcaagaagc tcaccaaagg
       61 tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag ttagaaatag
      121 acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa aaaaatctta
      181 tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga ttacttactc
      241 aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg aagaagcgaa
      301 tcttcccaat atcctagggg acaaaggata tattagccat aaaa
//
LOCUS       DAXWLB010000394          341 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.412, whole genome shotgun sequence.
ACCESSION   DAXWLB010000394 DAXWLB010000000
VERSION     DAXWLB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.412"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            44..166
                     /locus_tag="ACGE4H_002988"
     CDS             44..166
                     /locus_tag="ACGE4H_002988"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366303.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          124 a           45 c           64 g          108 t
ORIGIN      
        1 aaatctatat tttgatatac aaattacagc ttcagatact aagttgcata aaccttttcc
       61 ggaccccata caaaaggcaa ttgatgaatt gggcataaaa aataagaaac gatttatatt
      121 ggtgattcat tatatgacat gcaaagtgcg aaagcttgtg ggatagcatt tggtttagct
      181 aaatggggag ccaaaaatat agaaaatttt caaagtgtag atataattat tgattctcct
      241 caagaaatac ttgatttgtt agaaaaatag acaaagagcg tgagataagg atagggtctc
      301 acgctctttg tttttcgcca tttaaagatt gaaatttttg c
//
LOCUS       DAXWLB010000395          329 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.413, whole genome shotgun sequence.
ACCESSION   DAXWLB010000395 DAXWLB010000000
VERSION     DAXWLB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.413"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..318
                     /locus_tag="ACGE4H_002989"
     CDS             <1..318
                     /locus_tag="ACGE4H_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFF8366304.1"
                     /translation="IEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDK
                     KVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGK
                     IEI"
BASE COUNT          147 a           52 c           37 g           93 t
ORIGIN      
        1 attgaagaaa acaaattaat taagaataat aaagataaat acattttcat ctctaaaaat
       61 ggtaacccga tatctattca agcatataat caatctttaa gagcagtatc caaagaatta
      121 aacatagata aaaaagtttc ttctcacatg ttaaggcatt cccacatttc tctacttaca
      181 gaattaggaa tcccattaaa agccattatg gatcgtgtag gacatgaaga ttcaaaaaca
      241 acattaaaga tttatactca tacaactaaa aatatgcaaa atcaactagt agaaaaacta
      301 ggaaaaatag aaatttaaat tgccccttt
//
LOCUS       DAXWLB010000396          323 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.414, whole genome shotgun sequence.
ACCESSION   DAXWLB010000396 DAXWLB010000000
VERSION     DAXWLB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.414"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4H_002990"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACGE4H_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFF8366305.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DAXWLB010000397          321 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.415, whole genome shotgun sequence.
ACCESSION   DAXWLB010000397 DAXWLB010000000
VERSION     DAXWLB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.415"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>321
                     /locus_tag="ACGE4H_002991"
     CDS             <1..>321
                     /locus_tag="ACGE4H_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFF8366306.1"
                     /translation="AFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPY
                     SRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLL
                     NQCT"
BASE COUNT           94 a           66 c           60 g          101 t
ORIGIN      
        1 tcgcctttca gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat tgtgttgtcg
       61 ctgatgctgg gtataagaca cctaagtttg ttcacttttt gactcatttg aaccttcgac
      121 cttgtttgcc ttattcaagg cccaaaggga aaaaggagct attatcaaaa aatgagtttc
      181 tctatgacga gtattttgat tgttatattt gtccgcaaga tcaaatgctc gctttttcaa
      241 cggttactcg agaagggtac cgagaataca aatccaatcc aaaagaatgt gtgaactgtc
      301 ctctgctaaa ccaatgtacc a
//
LOCUS       DAXWLB010000398          320 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.416, whole genome shotgun sequence.
ACCESSION   DAXWLB010000398 DAXWLB010000000
VERSION     DAXWLB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.416"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(33..>320)
                     /locus_tag="ACGE4H_002992"
     CDS             complement(33..>320)
                     /locus_tag="ACGE4H_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFF8366307.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT           99 a           58 c           50 g          113 t
ORIGIN      
        1 ccgttccggg ctaccttttt aggtagccta ttttaatctt tgtatctttc tggatcaacg
       61 aaagtatact ttatatagtc ataacgccga tgatcgcttc gtgcgtctgg cacgtcagtc
      121 acgacatcaa acaaaaaata tacgtctttc ttcattctag ttttcgcagc aggaattttg
      181 aaatagttct tattagaata gtagagattg attaataagc tatcttcgat tgctaaaaag
      241 aaaacttctg aattccatac tttataaaaa tctttgataa atctattcga agggtcaaat
      301 ttaaaccata attgtgtctc
//
LOCUS       DAXWLB010000399          318 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.417, whole genome shotgun sequence.
ACCESSION   DAXWLB010000399 DAXWLB010000000
VERSION     DAXWLB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.417"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT          138 a           35 c           47 g           98 t
ORIGIN      
        1 acaaatttat aaaaaaacaa gataattgat actttgattt tgcgaacatt ttatctttcc
       61 gcctaattca gctttaatat catgattata tcagtaaaaa atcgtttttt tagctaattt
      121 aagcatcaaa taattattct taatcaatga aaaaataact aataaattaa ttttgggaat
      181 aggtaaaaac ttgttcggtt tctggataaa aataacatga aaaaggaaat ataaaataac
      241 atggagcgtg ggaggaataa agaaatttta cacgcaaggt gcgtaggaat gtaaagaaac
      301 ttaacacgaa acaaaagt
//
LOCUS       DAXWLB010000400          309 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.418, whole genome shotgun sequence.
ACCESSION   DAXWLB010000400 DAXWLB010000000
VERSION     DAXWLB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.418"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            1..309
                     /locus_tag="ACGE4H_002993"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACGE4H_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAXWLB010000401          303 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.419, whole genome shotgun sequence.
ACCESSION   DAXWLB010000401 DAXWLB010000000
VERSION     DAXWLB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.419"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..259
                     /locus_tag="ACGE4H_002994"
     CDS             <1..259
                     /locus_tag="ACGE4H_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFF8366308.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          114 a           56 c           53 g           80 t
ORIGIN      
        1 aaaatattca aaagcttgcg catcattgga tgatggattc gaagacgcct ttcaatatac
       61 cgtacaagga aattcccaca atcgactaaa gagtaccaat ctaattgaac gactgaatca
      121 agaagtacgc agaagagaaa agattattcg catcttcccc aatcaaacat cagccaatcg
      181 cttaattgga gccgttctta tggacctaca tgatgaatgg atttattctt caagaaaata
      241 catcaatttt gataagtaga aatggtaaaa acattgtata gcattttaca caggagtctg
      301 gaa
//
LOCUS       DAXWLB010000402          299 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.420, whole genome shotgun sequence.
ACCESSION   DAXWLB010000402 DAXWLB010000000
VERSION     DAXWLB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.420"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>299
                     /locus_tag="ACGE4H_002995"
     CDS             <1..>299
                     /locus_tag="ACGE4H_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8366309.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT          102 a           63 c           54 g           80 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAXWLB010000403          295 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.421, whole genome shotgun sequence.
ACCESSION   DAXWLB010000403 DAXWLB010000000
VERSION     DAXWLB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.421"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            76..>295
                     /locus_tag="ACGE4H_002996"
     CDS             76..>295
                     /locus_tag="ACGE4H_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFF8366310.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT          111 a           57 c           51 g           76 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctg
//
LOCUS       DAXWLB010000404          291 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.423, whole genome shotgun sequence.
ACCESSION   DAXWLB010000404 DAXWLB010000000
VERSION     DAXWLB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.423"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(2..73)
                     /locus_tag="ACGE4H_002997"
     tRNA            complement(2..73)
                     /locus_tag="ACGE4H_002997"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Gln,seq:ttg)
     gene            complement(83..155)
                     /locus_tag="ACGE4H_002998"
     tRNA            complement(83..155)
                     /locus_tag="ACGE4H_002998"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:His,seq:gtg)
BASE COUNT           82 a           78 c           70 g           61 t
ORIGIN      
        1 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct
       61 tggctatacc ccaagaataa agggcgaatg atgggaatcg aacccacgaa tgccggagcc
      121 acaatccggt gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt
      181 ctcaataaaa aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg
      241 cacgccctca acgggcactg gattttgagt ccagcgcgtc tgccaattcc g
//
LOCUS       DAXWLB010000405          281 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.424, whole genome shotgun sequence.
ACCESSION   DAXWLB010000405 DAXWLB010000000
VERSION     DAXWLB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.424"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            157..>281
                     /locus_tag="ACGE4H_002999"
     CDS             157..>281
                     /locus_tag="ACGE4H_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFF8366311.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          115 a           27 c           53 g           86 t
ORIGIN      
        1 ctggaataaa tggtttatat cgagcagatt atggagataa atctcaattt taatgatact
       61 tataatatta aatatgtctc aaaatttttt gattagaaaa ataggtatat tgaatcagtg
      121 acataaaagt atttatagtt tgtaggggga atcgtaatgg aagaaaaaaa tagactaaat
      181 gctaaacaat gttcagatca agagcttaaa aaaattaaag gaggtgctgg aacaaaacct
      241 caaggaaaac cggcatcaaa tttagtggaa tgtgtttttt c
//
LOCUS       DAXWLB010000406          280 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.425, whole genome shotgun sequence.
ACCESSION   DAXWLB010000406 DAXWLB010000000
VERSION     DAXWLB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.425"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>280
                     /locus_tag="ACGE4H_003000"
     CDS             <1..>280
                     /locus_tag="ACGE4H_003000"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFF8366312.1"
                     /translation="DDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPD
                     GKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGN"
BASE COUNT          104 a           26 c           44 g          106 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc
//
LOCUS       DAXWLB010000407          273 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.426, whole genome shotgun sequence.
ACCESSION   DAXWLB010000407 DAXWLB010000000
VERSION     DAXWLB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.426"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            1..273
                     /locus_tag="ACGE4H_003001"
                     /pseudo
     CDS             1..273
                     /locus_tag="ACGE4H_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          108 a           46 c           53 g           66 t
ORIGIN      
        1 cattcgagaa tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac
       61 gagtggttca ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact
      121 acgtaaaaga aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat
      181 attcggacga agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg
      241 tgcagaatat taggtctatc acgtcagtcc tat
//
LOCUS       DAXWLB010000408          272 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.427, whole genome shotgun sequence.
ACCESSION   DAXWLB010000408 DAXWLB010000000
VERSION     DAXWLB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.427"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT           89 a           46 c           38 g           99 t
ORIGIN      
        1 agaataacag acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag
       61 caaaatatta aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga
      121 tctatcttta cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt
      181 ggatacgaac cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt
      241 ttagacaaac caaaaaagag gttgtgacaa ta
//
LOCUS       DAXWLB010000409          272 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.428, whole genome shotgun sequence.
ACCESSION   DAXWLB010000409 DAXWLB010000000
VERSION     DAXWLB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.428"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
BASE COUNT           90 a           28 c           54 g          100 t
ORIGIN      
        1 gagggatgca gatggatcaa aaaagaattc atgtaggact gattactagt tttgtcacga
       61 tgcttattta tttggatcaa ggtcatttga cttggacagt agttggtgtc gtaggatttt
      121 tgacattatt cacagcaata aaaaaataaa catctttatg taatctgatg ttctatctat
      181 ttaagaaaag catgtttaca gcagtatgat tctgttgtaa acgtgttttt atttgtggaa
      241 aatttaattt ttagaaaagt gtatcgaaaa at
//
LOCUS       DAXWLB010000410          266 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.denovo.430, whole genome shotgun sequence.
ACCESSION   DAXWLB010000410 DAXWLB010000000
VERSION     DAXWLB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.denovo.430"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..>266
                     /locus_tag="ACGE4H_003002"
     CDS             <1..>266
                     /locus_tag="ACGE4H_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFF8366313.1"
                     /translation="KVSVTLSSNSRQAWNQANLLLNEKIAEKIKRNEELPLTFGELKT
                     KWDEKYKPTVKESSYRTTQVYLSLISKYIKDDVLVKNVNSNLIQ"
BASE COUNT          117 a           39 c           37 g           73 t
ORIGIN      
        1 aaagtgtccg ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt
       61 ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga
      121 gaattaaaaa ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga
      181 acaactcagg tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa
      241 aacgttaata gtaatcttat acaaga
//
LOCUS       DAXWLB010000411         8070 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.126, whole genome shotgun sequence.
ACCESSION   DAXWLB010000411 DAXWLB010000000
VERSION     DAXWLB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.126"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..1658
                     /locus_tag="ACGE4H_003003"
     CDS             <1..1658
                     /locus_tag="ACGE4H_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFF8366314.1"
                     /translation="LDFPSSEIFYYQQQQFTFPIDTSMNVEIVANRKALSTVRNKKKE
                     LKDLDNHAWQSDNETSSNVAEALESVNELETNLDQSKESMYKLSYVVRVSANDLDELK
                     RRCNEVKDFYDDLSVKLVRPFGDMLGLHEEFLPASKRYMNDYIQYVTSDFLAGLGFGA
                     TQMLGENEGIYVGYSLDTGRNVYLKPALASQGVKGSVTNALASAFVGSLGGGKSFANN
                     LIVYYAVLYGAQAVIVDPKAERGRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMK
                     NTKDSESLAIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTP
                     LSTSIADHIESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYT
                     TMELLSVAMLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRA
                     MNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRL
                     RDLENGQCLISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            1661..3844
                     /locus_tag="ACGE4H_003004"
     CDS             1661..3844
                     /locus_tag="ACGE4H_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFF8366315.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3841..4842
                     /locus_tag="ACGE4H_003005"
     CDS             3841..4842
                     /locus_tag="ACGE4H_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFF8366316.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4857..5771
                     /locus_tag="ACGE4H_003006"
     CDS             4857..5771
                     /locus_tag="ACGE4H_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFF8366317.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            6085..6132
                     /locus_tag="ACGE4H_003007"
                     /pseudo
     CDS             6085..6132
                     /locus_tag="ACGE4H_003007"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6148..8067
                     /gene="tet(M)"
                     /locus_tag="ACGE4H_003008"
     CDS             6148..8067
                     /gene="tet(M)"
                     /locus_tag="ACGE4H_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFF8366318.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         2582 a         1491 c         1721 g         2276 t
ORIGIN      
        1 aactggactt cccgtcctct gaaatcttct actaccagca acagcaattt acctttccga
       61 ttgatacgtc aatgaatgtg gaaattgtag cgaatcgtaa agccctatct actgtccgca
      121 ataaaaagaa agaactgaaa gacttggata accacgcttg gcaaagtgat aatgaaacca
      181 gctctaatgt ggcggaagct ctggaaagtg tgaacgagct ggaaaccaat ttagaccaaa
      241 gcaaggaatc tatgtataag ctgtcctatg ttgtaagggt atcagcaaat gaccttgacg
      301 aacttaaacg tcgttgtaat gaagtgaaag atttctatga tgatttgagc gtgaaactgg
      361 tacgaccttt tggggatatg ctgggcttac atgaagaatt tttacctgcc agcaaaagat
      421 atatgaatga ctatattcaa tacgtgacct ctgatttcct cgctggttta ggttttggtg
      481 ctactcaaat gctgggtgaa aatgagggga tttatgttgg ctacagctta gatactggac
      541 gcaatgtcta tctgaaacct gctcttgcca gtcaaggggt taagggttca gtaaccaatg
      601 cgttagcgtc tgcctttgtc ggttcgctgg gtggtggtaa atcctttgcg aataacctta
      661 tcgtctatta tgcagtgctt tatggggcac aagcagtgat tgtagaccca aaagcagaac
      721 gtggcagatg gaaagaaacc ttgccagaga tttcccatga aatcaatatc gtcaatctga
      781 cttctgatga gaaaaacaaa ggcttacttg acccttatgt aattatgaaa aataccaaag
      841 attctgaatc actggctatt gatattttga cattccttac ggggatttcc tctcgtgatg
      901 gggaacgctt cccaatcctt agaaaagcca ttcgtgcagt aaccaatagt gaagtgcgag
      961 ggttgatgaa agtgattgaa gaattacggg ttgagaatac gccactaagt accagcatag
     1021 ccgaccatat cgaaagtttt acagactatg actttgccca tctgcttttt agtgatggtt
     1081 atgtagagca gtctatcagc cttgaaaaac aactgaacat tatacaggtt gccgacttgg
     1141 tacttcctga caaggaaact tcctttgagg aatataccac aatggagtta ctatcggtag
     1201 caatgctgat tgtcattagt acctttgcgt tggactttat ccatacagac cgaagtattt
     1261 tcaagattgt agacttagac gaagcatgga gcttcttaca ggtggcacaa gggaaaacac
     1321 tatctatgaa gctggttcgt gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc
     1381 aaaatacaga tgacctctta gatgaaaaac tgaaaaataa cctcggctta aaatttgcct
     1441 ttcgttccac tgaccttaac gagattaaaa aaacccttgc cttttttggt gtagacccag
     1501 aggacgaaaa caatcagaag cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg
     1561 atttatatgg tcgtgtcggt gtgatacagt tccaccctgt atttgaagaa ctgctccatg
     1621 cctttgatac cagaccacct gtgcgaaaag aggtgtaaat gtgaaaccat caatattaaa
     1681 cagaataaaa tcaaactgga cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt
     1741 cacacttgtg attgccattt ttcttttagc catgctggga acggtggttc aagctgcggg
     1801 cttggtagat gatacggtca atgtggcaaa tgaatacagc cgatacccac ttgaaaacta
     1861 tcaactggat ttttatgtgg ataatagctg gggctggcta ccgtggaact ggtcggacgg
     1921 aattggaaaa caagtcatgt atggactata tgccattacc aattttattt ggacaatcag
     1981 tctttatgtt tccaatgcga cgggttactt agtacaggaa gcctattcct tagacttcat
     2041 ttccgctaca gcagattcca ttggcaagaa tatgcagacc cttgcagggg ttacgcctag
     2101 tggactatca acagagggtt tctatgttgg attcctttta ctcttgattt tggttcttgg
     2161 ggtttatgtt gcctatacag gactgataaa gagagaaacc acaaaggcaa ttcatgccct
     2221 catgaatttt gtgctggttt ttatcctatc ggcttccttt attgcctacg ctcccgacta
     2281 cattaaaaaa atcaatgact tttcatcaga catcagcaac gctagtttgt cactgggtac
     2341 aaaaattgtc atgccccatt cagatagtca aggcaaggac agtgtggact tgatacgtga
     2401 cagcctattt tccatacagg ttcagcaacc gtggctactg cttcaatata acagttcaga
     2461 cattgaaagt attggtattg accgtgtgga aagcctgctc tcaaccagtc cagattccaa
     2521 caatggggaa gacagagaaa aaattgttgc ggaagaaatt gaagatagaa gcaataccaa
     2581 tatgacgatt acaaagacga ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa
     2641 tattgggatt tccatatttg tattcctatt aacaggaatc atgattttct cgcaggtact
     2701 ttttatcatc tatgctatgt ttctgcctgt gagctttatt ttaagcatga ttccatcatt
     2761 tgatggtatg tcaaaacgag ccataacaaa gctctttaat accattttga cacgagctgg
     2821 aatcacattg attattacga cagcatttag tatttcaacc atgctctata ccttatcggc
     2881 tggttatccg ttctttttga ttgcttttct acagattgtg acctttgcag gaatctactt
     2941 taagctgggc gatttaatga gtatgttttc tctacagagt aacgattctc aaagtgtggg
     3001 aagtcgtatg atgagaaaac ctcgtatgct tatgcacgct cacatgcacc gtctacagcg
     3061 gaaacttgga cgctccgtga ctgctttagg ggctgggtct gccattgcca gtgctacggg
     3121 taaaaaagga cagtcgggtt cgggaagttc tgcgagtaca caagcagatc actctcgacc
     3181 aaacgggcag gaaaaatcaa cactaggaaa gcgtatcggt caaaccatcg gtacagtagc
     3241 tgataccaaa gacagaatgg tagatactgc tggtaatttg aaagaacagg ttaaggattt
     3301 gccgaccaat gcaagatatg cagtatatca aggaaaatct aaagtgacgg ataatgtcca
     3361 tgatttaacc agaagtattt ctcaaaccaa agcggataaa gccagtggac gcaagaaaca
     3421 gcaggaacaa agacggaaaa ccattgcgga gcgtcgctct gaaatggaac aggtcaaaca
     3481 gaaaaaacag ccagcttctt ccgttcatga aagaccagcc actaagcaag aacaatctca
     3541 tgatggacag acttcaaggc aaacaactgt acaggcttcc tatagggaat ctcaacaagc
     3601 caaacaagag cgtccaacag ttaagtccga ttcttcaagt ttaaaaacgg aacgtcaaag
     3661 taatacagtt caagaaagaa cggttcaaaa gccagtaact tcaactgcac caacagatag
     3721 agcttcacaa cgttcaatca caaaagaacg tcagtctact gttcaaagag taccactaca
     3781 aaatacaaaa agtagaccac caatcaaaac cgccaccatt aagaaaggca gtaagaaacc
     3841 atgaagctga aaactttagt gattggtggt tcgggattat tcttgatggt cttctcactg
     3901 cttctgtttg tcgccatttt attttcagat gaacaagatg gcggtttttc caacattcac
     3961 tatggcggtg tggatgtttc cgcagaagtg ctggctcata agcctatggt agaaaaagtt
     4021 gccaaagaat atggaattga agaatacgtc aatatacttc ttgcgattat acaggtggaa
     4081 tcgggtggta cagcagaaga tgttatgcag tcctcggaat ccctcggtct tccacctaat
     4141 tcattgagta cagaagaatc cattaagcaa ggtgtgaagt atttcagtga gttattagcc
     4201 agtagcgaaa ggcttggtgt agatttagaa tcggttatcc agtcctacaa ttatggtggt
     4261 ggtttcttag gatatgtggc taatcgtggg aataaatata cctttgaact ggctcaaagt
     4321 ttctcaaaag agtattcggg tgacgaaaaa gtgtcttacc ccaatcccat agccatacct
     4381 atcaatgggg gctggcgata caattacggg aatatgtttt atgtgcaact ggtaacgcag
     4441 tatcttgtca caacagagtt tgatgatgat acagtacaag ccatcatgga cgaagctctg
     4501 aaatttgaag gctggaagta tgtctatggc ggagcttccc cgactacctc ttttgattgt
     4561 agcggactga cacaatggac gtatggaaaa gctggaatca acttaccacg aacagcacaa
     4621 cagcaatatg atgtaacccg gcatatccca ttattggaag caaaagccgg cgatttggtt
     4681 ttctttcatt ctacctataa cgctggctct tatattactc atatcgggat ttatttaggc
     4741 gataaccgta tgtttcatgc aggcgaccct atcggttatg ccgacttaac aagctcttac
     4801 tggcaacagc atttagtggg agcaggacga atcaaacaat aagaaaggac gattcaatga
     4861 tgaaattcgg aaaaaatcag aatacagaaa aacagacacc aaaggaaaag aaacctcgtg
     4921 tctataaggt caatcctcgt aaaaaggttg tgattgtcct atgggtactt ttaggactta
     4981 gtttcagctt tgcgatattc aagcacttta cagccattga tacccatacc attcatgaaa
     5041 ccaccatcat agaaaaggaa tatgtcgata ctcatcatgt ggaaaatttt gtagagaact
     5101 ttgcgaaagt ctactattca tgggaactaa acgacaagtc cattgataat cgcatagaaa
     5161 acctgaaagc atatctgaca gaggaacttc aagctctgaa tgtcgataca gttcgtaaag
     5221 atattcctgt atcgtcttct gtaaaaggat ttcagatatg gacggtagaa gcagatggcg
     5281 acaatcaatt tgatgtaacc tacagtgtag accaactcat tacagagggg gaaaatacaa
     5341 agaccgtcca ctctgcttat atagtgagtg tctatgtaga cagtactgga aatatggtcc
     5401 tcattaagaa tccgaccatt actagcatac caaagaaatc agactataaa ccaaaagcta
     5461 ttgaaagtga gggtacggtt gattccatta caaccaatga aatcaatgag tttttaacga
     5521 cgttcttcaa gctctatcct acagcgacag ccagtgaact ttcctactat gtgaatgacg
     5581 ggatattaaa accaatcgga aaagagtaca tctttcaaga actggtaaat cctatttaca
     5641 atcgtaagga taatcaagtc acggtatcgc tgacagtgga gtatatcgac cagcagacca
     5701 aagcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
     5761 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
     5821 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
     5881 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
     5941 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgca cccagtttaa
     6001 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
     6061 atggttatgc ataaaaatcc cagtgataag agtatttatc actgggattt ttatgccctt
     6121 ttgggctttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
     6181 gttgatgcgg gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
     6241 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
     6301 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
     6361 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
     6421 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
     6481 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
     6541 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
     6601 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
     6661 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
     6721 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgc
     6781 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
     6841 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
     6901 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
     6961 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
     7021 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
     7081 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
     7141 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
     7201 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
     7261 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
     7321 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
     7381 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
     7441 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
     7501 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
     7561 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
     7621 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
     7681 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
     7741 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     7801 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     7861 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     7921 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     7981 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     8041 cgatatatgt tcaataaaat aacttagtgt
//
LOCUS       DAXWLB010000412         7776 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.129, whole genome shotgun sequence.
ACCESSION   DAXWLB010000412 DAXWLB010000000
VERSION     DAXWLB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.129"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            241..1209
                     /locus_tag="ACGE4H_003009"
     CDS             241..1209
                     /locus_tag="ACGE4H_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFF8366319.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4H_003010"
     CDS             1202..2233
                     /gene="vanA"
                     /locus_tag="ACGE4H_003010"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFF8366320.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2239..2847
                     /locus_tag="ACGE4H_003011"
     CDS             2239..2847
                     /locus_tag="ACGE4H_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFF8366321.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4H_003012"
     CDS             3275..4186
                     /gene="vanY"
                     /locus_tag="ACGE4H_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFF8366322.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4H_003013"
     CDS             4339..4824
                     /gene="vanZ-A"
                     /locus_tag="ACGE4H_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFF8366323.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5111..5440)
                     /locus_tag="ACGE4H_003014"
     CDS             complement(5111..5440)
                     /locus_tag="ACGE4H_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFF8366324.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5745..6074
                     /locus_tag="ACGE4H_003015"
     CDS             5745..6074
                     /locus_tag="ACGE4H_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFF8366325.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6064..6351
                     /locus_tag="ACGE4H_003016"
     CDS             6064..6351
                     /locus_tag="ACGE4H_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFF8366326.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6630..7193)
                     /locus_tag="ACGE4H_003017"
     CDS             complement(6630..7193)
                     /locus_tag="ACGE4H_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFF8366327.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2566 a         1296 c         1545 g         2369 t
ORIGIN      
        1 tggtgtcatc caccaacatc aaaaagtata gagccataat tttttaggaa aatctcaagg
       61 ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc tcgttcttac
      121 acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa gatttgacaa
      181 gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt ggtcttaatt
      241 atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga tgcattccat
      301 gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt gtcggaatcc
      361 aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc agagatttcc
      421 gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac ccgaagcatc
      481 ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt cgacaatgtg
      541 gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat ggcagtacgc
      601 aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt
      661 ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat aggcaaagcg
      721 gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg cagccgaagt
      781 atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat cgttacgctt
      841 catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat acagagaatg
      901 aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac ctatgagttg
      961 gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt ggaaggagag
     1021 gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt tttacttaaa
     1081 cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac cgagcaagcg
     1141 ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag gagacaggag
     1201 catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc atgacgtatc
     1261 ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg agccgttata
     1321 cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga
     1381 aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg gattacttgt
     1441 taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag ctttgcatgg
     1501 caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta tcccttttgt
     1561 aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat acatcgttgc
     1621 gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg ataggccggt
     1681 ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct catccttcgg
     1741 tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg caagacaata
     1801 tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt
     1861 gggaaacagt gccgcgttag ctgttggcga ggtggaccaa atcaggctgc agtacggaat
     1921 ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag ttataaccgt
     1981 tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa aaaaaatata
     2041 taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag ataacggccg
     2101 cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc gttatccccg
     2161 tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga tcgtattagc
     2221 gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat agtacacggt
     2281 gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc ggttgacggt
     2341 tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt gaaggcaaaa
     2401 gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg tcctaatcgt
     2461 gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac aaaggaaagt
     2521 tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc ttcaaaatca
     2581 agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac gggtgagctt
     2641 gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc ggcaaatgga
     2701 atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg
     2761 tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc ataccccaat
     2821 agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa actatataag
     2881 ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat ttatatatag
     2941 tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc gtgcgctgcg
     3001 gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac accgcccatt
     3061 gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga aaattcatct
     3121 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
     3181 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
     3241 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
     3301 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
     3361 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
     3421 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
     3481 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
     3541 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
     3601 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
     3661 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
     3721 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
     3781 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
     3841 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
     3901 aattcaatat gaactatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
     3961 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
     4021 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     4081 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     4141 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     4201 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     4261 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     4321 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     4381 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     4441 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     4501 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     4561 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     4621 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     4681 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     4741 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     4801 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     4861 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     4921 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct tacctaacac tatagtaaca
     4981 aagaaagccc aattatcaat tttagtgctg aggaattggt ctctttaata aatttcctta
     5041 acgttgtaaa tccgcatttt cctgacggta cccccaaact aagatttcat agatagattt
     5101 tctgataact ttaaaaaaca cgaatagcgg aaaggatgtc ttcattcgct aaagagtaat
     5161 aaactacttt tccatcttga cgtgattttg ccattccatg tttttttaaa aaacgtaaat
     5221 ggtgtgacgt tgtagcgaca ctagcagtaa gtatttctgc taaatcacag acgcacattt
     5281 ccttataggt ttctaaagca tagaaaattt taattctaga aaaatcgctg aaacatttcc
     5341 ctaacacaag taagtttgaa aaatcttttt gatccaattt attttttatt ccatttactt
     5401 tttctttatg tacgacagtc acttggcaaa cttttttcat tcatctcacc tcacattcaa
     5461 acgattattt gaatgaagta tacctatttg atcactcttt tgtcaatcta ccttaattcc
     5521 aatacactat tataagcaat ttcatgtatt gaagtaaact tacttgcaag tgcgtttttg
     5581 attccaagtt aaaaacatat tattattcta cataatttcc taatcaaata aaactccttt
     5641 gaaaattgtt ctaaaggagt tttattattt tgtatttcta ctaaaaatag cgtacaatgt
     5701 aattactaag taattacgaa atgtaaaata atggaggaaa taatatgatt acaaaaacaa
     5761 gaaaacaagg aaattctatt atgttaacgg tccctaaaga attcgatgtg cccaatggtg
     5821 ttgaagtaga agctaagctt gtagagaatg gtatccttta tgagtttgta gagcctaaaa
     5881 aagaattttt tgattttagt gaagatattt tggctgatat tctttcagaa ggttatagca
     5941 aacaagagat cttaaaagaa tttagaaata gaaaaaatgc gttaacttct gcatttaaat
     6001 ctattgctga agatactgtt gcaaattcta aaccgatgac aaaagaggag ttggcagcag
     6061 aaattggctt atgagattct accttctaaa cacgtaataa agtatctaaa aaaattaaaa
     6121 gagaaaacat taaaagaaca atttttaacg ataatttatg atgagatagc agttcgtcct
     6181 cattcgggag aacaaaaaac gggtgattta tcaggaattt gggctattgg attcaagtat
     6241 gcaggtacga catatcgcgt tgcatatgaa attaaagata atgcagtgat acctaactta
     6301 ctatgtggaa cgcatgaaaa tttttatgaa caattaaaaa agattaggta agaacccaat
     6361 gtttcaactt gagaggatag acgacacttt aaaagataat ttttaacagc tgagagttgt
     6421 aataccctat aattttaaac cactaaccta tatatttatg aaaaatagaa aaaattaaaa
     6481 cgcattttaa gcctctttca aattaaaagc ttcatcctta tgcacaaaga gggcttaaaa
     6541 tgcgttattt tatattgttt tttagtaata ataaaaaaat cactctaatt ctcccataaa
     6601 tattatagat tcctaaagat taaaatagat tattccctat tgtcttttaa gtagcggtag
     6661 agtgttgcct tgctaatacc agtcatttcc gtaatttctt ttacgctata aacctgactt
     6721 ttatacaatt ttactgctcg atcaacatct ttctggttca ctcgtggacg gccaccttta
     6781 cgacctctcg cacgtgcact tttcaaacct tctttggtac gttcaacaat taaatcacgt
     6841 tcgaactgac taaatgcttg gaagacagtc atcattaagc gtccgtgagg cgtgctggta
     6901 tcgaagtttt ctttcaagct caccagctta acatggtgtt cttcaaaata ggtaacaagg
     6961 tcaatcaaat cctttgtact acgacccaat cgagaaaaac tttctacaat taccgtatct
     7021 cccggacgaa ttttatcttt taaacgattt agttggggac gaaccgcttt ggttcccgtc
     7081 attttctcag ttaggatttc attacaattt tgttcattga gtaagtctaa ttgacgattt
     7141 agttcttgtg aacgtgtact cactcgggca taaccataaa tgtatccact catgcttttc
     7201 ttcctttcgt actttaattc tggcttgatt gtatcataaa agggagttat agatacatag
     7261 ttaatgcaac aagttatgat acaaaatatg acctctttta tttaataaaa gaggtcattt
     7321 cattttgtct cataatcgat tgtttttgat acgagtgtgt atacgaaata aacaaaaaaa
     7381 tcttaagtaa atttctagat acctaattta atcctaacta gtgtatccta aagttaataa
     7441 cgagtttatc acaatgaaaa ttataaaatg ctcccatatt agatataaca tgcaatattt
     7501 gaattagaac tacaaaattt attcgtttta taaagtaaat aatgttgaga tactaaggaa
     7561 tagaatatta taaggaattg agctgaatgt attggataaa aaaagaataa ctttattaga
     7621 gatggactat aaaaatgctc gtaattttct tctgaagtct gaaagctatt tgaatgctca
     7681 gctaccagaa tatattagtt ttgacgaaac tttaaaatct gctaaaaaag ttttaacttc
     7741 gccgtttgta taattaagcg agacaaataa aaaagc
//
LOCUS       DAXWLB010000413         7477 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.135, whole genome shotgun sequence.
ACCESSION   DAXWLB010000413 DAXWLB010000000
VERSION     DAXWLB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.135"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            108..191
                     /locus_tag="ACGE4H_003018"
     CDS             108..191
                     /locus_tag="ACGE4H_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFF8366328.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE4H_003019"
     CDS             316..1053
                     /gene="erm(B)"
                     /locus_tag="ACGE4H_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFF8366329.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1223..1483
                     /locus_tag="ACGE4H_003020"
                     /pseudo
     CDS             1223..1483
                     /locus_tag="ACGE4H_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1646..2311
                     /locus_tag="ACGE4H_003021"
                     /pseudo
     CDS             1646..2311
                     /locus_tag="ACGE4H_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2347..2592
                     /locus_tag="ACGE4H_003022"
     CDS             2347..2592
                     /locus_tag="ACGE4H_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFF8366330.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2695..3564
                     /locus_tag="ACGE4H_003023"
     CDS             2695..3564
                     /locus_tag="ACGE4H_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFF8366331.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3545..4279
                     /locus_tag="ACGE4H_003024"
     CDS             3545..4279
                     /locus_tag="ACGE4H_003024"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFF8366332.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4312..5220
                     /locus_tag="ACGE4H_003025"
     CDS             4312..5220
                     /locus_tag="ACGE4H_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFF8366333.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5217..5759
                     /gene="sat4"
                     /locus_tag="ACGE4H_003026"
     CDS             5217..5759
                     /gene="sat4"
                     /locus_tag="ACGE4H_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFF8366334.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5852..6646
                     /locus_tag="ACGE4H_003027"
                     /note="aph(3')-IIIa"
     CDS             5852..6646
                     /locus_tag="ACGE4H_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFF8366335.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6922..7464
                     /locus_tag="ACGE4H_003028"
     CDS             6922..7464
                     /locus_tag="ACGE4H_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFF8366336.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2622 a         1129 c         1566 g         2160 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattt tgtgtataat aggaattgaa
       61 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
      121 aaatgcgtaa tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
      181 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
      241 agataactaa aattacaaac aaatcgttta acttctgtat ttatttacag atgtaataac
      301 ttcaggagtg attacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
      361 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
      421 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
      481 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaacatt
      541 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
      601 aaaattgttg ggagtattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
      661 gaaagccatg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
      721 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
      781 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
      841 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
      901 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
      961 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     1021 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt ttaaatttgg
     1081 aaagttacac gttactaaag ggaatggaga taaattatta gatatactac tgacagcttc
     1141 caagaagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag gggtacaaat
     1201 tcccactaag cgctcgggac cccttgtagg aaaatgtcct aagtgtggca acaatattgt
     1261 attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa tgtaagttta ctttagctga
     1321 acattttaga aagaaaaaac taaccaaaac gaatgtaaaa gaattactgg agggaaaaga
     1381 aaccctggta aaaggaatca aaaacaaaga gaaaaagccc tacaatgccg ttgtaaaaat
     1441 tggggaaaag ggatatattg attttatctc tttttcaaaa taaacatggg gctgacccaa
     1501 aagtagctcc tatatgaaaa atagaggtct ttccatttag atagtagtgg aaagacctct
     1561 atttatcttt tgaaagtgaa tacaaaaata aagcacactc tgctataata taggtgatta
     1621 atccatttga gagaggtgct ttatcatgta tcaaaattat accataaacc aactttgttt
     1681 gcctatagat cttgaaatta aattagaaga aaatgatttt gctcatgcca ttgtgcagtt
     1741 tgttgactct attcccgatg aagtctttct tccttattat caatctatgg gtagacccca
     1801 atatcatcct cgtatgctac tttccattat tctttgtgcc tatattcaag gggtttattc
     1861 gggccgccaa atacaaaata tgctgataga tagtattcgg atgcgctact tatctcaaga
     1921 gcaattccct aactttcgta ctattaatcg ttttcgcgtt catccgatta tgaacgatat
     1981 tctagatcat tcatttgttc aatttcgtga gctactggtt caaagtggct taatcagtgg
     2041 cagtgctcta tacattgatg gtacaaaaat tgaagcggat gcgaataaat actctttcgt
     2101 ttggaaaaaa agtattttaa agtataagga tagccttgat caaaaagccc ttgaaaacta
     2161 ccaacaaatg gtggaaacaa aaatcttacc tgagttaata gatgatttaa aggatgaact
     2221 ttctatagaa gagattgaaa ggattcgtca atctttggaa gaaaaggaaa atcaattaat
     2281 tgaagcgatt gaccaaaccg aaacagtaga agggggctga cccattatta ataaggagtg
     2341 gacaacatga ttgatgtaaa tgagctattg gaagaagcta ttagagaaac tgaaaatcta
     2401 aatgatggcg aagtttttct tgttaaagat ttattcaagg gatatgtggg gaataggata
     2461 cccagaaagg atcgacttct gcttggaacg ttatttttaa accatgttag caaaatgaat
     2521 ggtaatttaa aggctattga aaagacttca tctaatcagc agaggtataa aaagacaatt
     2581 gataagtttt aactatcttt tgtgaaatta aaaaaattat aaatatgttg agtagaagca
     2641 aaagatttgg cttggaattt ttacagcagt gaatcgataa aaggagcatg cttgatggta
     2701 gataatatta ttaaatcagt agcagagaaa ttatcctctc tgtcttatat agaaggtatt
     2761 gttttaggtg gttcacgtgc aaggggcacc catacagagg attcggatat agatatcggc
     2821 atctattaca attcagaatc atttgacata aatactatta atcaattcgc tacaaagctg
     2881 gatgatgagc atagaaataa ccttgttgta cctcccggag catggggtga ttggattaat
     2941 ggcggcggat ggttagtcat aaacgggtat catgtggatt taattttacg tgatattaaa
     3001 cgtgtggaac aaataatgaa agatacagag cacggaattg ttactgccaa ttatcagact
     3061 gggcatcccc atggttatat tagtgcaatg tatcgaggag aattagcgat tagcaaaata
     3121 ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac aggcagaaac ttatcccaat
     3181 gctttgcaga aaagtttagt taactttttt atgtttgaag cagggttctc tttaatgttt
     3241 gtaaaagcaa attcgggaac agacgataaa tattatattg cgggtcatgt ttttcgtata
     3301 gtttcatgtt taaatcaagt gttatttgca tgtaataatg cttattgtat caacgaaaag
     3361 aaagctataa aactgcttga aacttttgaa cataaacctg aaaaatatac cgagaaggta
     3421 aatcatattt ttgaagtact cggtatctca ctttttgaat gctacgacat gaccgagaag
     3481 ctttataatg aagtgaatga aattgtatcg gagataaata actttttaaa cgaggagagt
     3541 tcagatgaaa gaaaacaaat atgatgataa tatatttttt caaaaataca gtcaaatgag
     3601 tcgctcgcag aaaggactgg ctggtgcggg agaatgggag actttgaaaa agatgctacc
     3661 tgattttaag ggtaagcgtg tgcttgattt aggatgcggc tatggatggc actgtatata
     3721 tgcgatggaa aacggtgctt cctctgtagt aggtgttgat atttctcata aaatgctcga
     3781 agtagcaaaa ggaaaaaccc attttccaca gattgaatat gaatgctgtg ccatagaaga
     3841 tgtggatttc ccagaggaga gctttgatgt aatactaagt tcgcttgcgt ttcattatgt
     3901 agcagactat gagaatttaa taaaaaagat atataggatg ctgaaggctg gtggcaattt
     3961 agtttttaca gttgaacatc ctgtttttac tgctcatgga acacaagact ggtattataa
     4021 cgaaaaagga gaaatactgc atttcccggt ggacaattat tattatgagg gcaaacggac
     4081 agctatgttt ttggaagaaa aggttacaaa atatcataga acactgacca catatctaaa
     4141 tacactgctt tcaaatagtt ttataataaa tcagattgtg gagccacagc cgccagagaa
     4201 catgatggat attccgggga tggcggatga aatgcgacgc ccaatgatgc tgattgtatc
     4261 ggcaaaaaag aagatgtaat aatatagaaa aaataaacga ggagtatgta aatgagatca
     4321 gaaaaagaaa tgatggattt agtactttct ttagcagaac aggatgaacg tattcgaatt
     4381 gtgacccttg aggggtcacg cgcaaatatt aatataccta aagatgaatt tcaggattat
     4441 gatattacat attttgtaag tgatatagaa ccgtttatat ctaatgatga ctggcttaat
     4501 caatttggga atataataat gatgcaaaag ccggaggata tggaattatt cccacctgaa
     4561 gaaaagggat tttcctatct tatgctattt gatgattaca ataaaattga tcttacctta
     4621 ttgcccttgg aagagttaga taattaccta aagggcgata aattaataaa ggttctaatt
     4681 gataaagatt gtagaattaa aagggacata gttccgactg atatagatta tcatgtaaga
     4741 aagccaagcg caagggagta tgatgattgc tgcaatgaat tttggaatgt aacaccttat
     4801 gttattaaag gattgtgccg taaggaaatt ttatttgcta ttgatcattt taatcagatt
     4861 gttcgccatg agctgctgag aatgatatca tggaaggtcg gcatcgaaac aggctttaaa
     4921 ttaagtgtag gcaagaacta taagtttatt gaaaggtata tatccgagga tttgtgggag
     4981 aaacttttgt ccacctaccg gatggattcc tatgaaaaca tatgggaagc attatttcta
     5041 tgccatcaat tgttcagggc ggtatccggt gaggtggcgg aaaggcttca ttatgcctat
     5101 ccggagtatg ataggaatat aacaaaatat accagggaca tgtataaaaa atacactggt
     5161 aaaaccggct gcctggatag cacatatgcc gctgatatag aagagaggcg ggaacagtga
     5221 ttacagaaat gaaagcaggg cacctgaaag atatcgataa acccagcgaa ccatttgagg
     5281 tgataggtaa gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct
     5341 atgaagcgcc atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag
     5401 attgccttga atatattgac aatactgata agataatata tctttactac caagacgata
     5461 aatgcgtcgg aaaagttaaa ctgcgaaaaa attggaaccg gtacgcttat atagaagata
     5521 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
     5581 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
     5641 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
     5701 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     5761 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     5821 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     5881 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     5941 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     6001 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     6061 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     6121 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     6181 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     6241 tccctatacg aatagcttag acagccgatt agccgaattg gattacttac tgaataacga
     6301 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     6361 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     6421 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     6481 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     6541 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     6601 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     6661 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     6721 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat agagttctat
     6781 attagagatg taaagagttt tggtgaagag gtttgtgagt atggctttta taaccagggt
     6841 gcaatgagaa gcactaacag cagctagctt aagaacgcat aggatatact ttttatggaa
     6901 gtcacagagg aggaataaaa tttgaacagg attaacagag taacctctat tcttattcag
     6961 ctgcaatcaa aaaaaataat tcctgccaaa gaaattgcac agcgatttaa tataagctta
     7021 aggacagttt acagagatat ccggacactt gaagaagccg gaataccaat tggatctgag
     7081 gccggaaaag gatattttct tgtagagggc ttcctacttc cgccggtaat gtttacagcg
     7141 gcggaagtgg gtgcattgat tacagcaggg aaatttttaa attgccatgg agatgaatca
     7201 tttataaagg attttgattc agctatgtat aaaatcaaat ctattttgaa gcatggtgaa
     7261 aaaaactatg cacaggagtt ggagaacagt attaacgtgt acagcacatc tggacagaaa
     7321 aatacattgg cggataacgt tattgcagca attcagactg caatctgcaa taaaagggta
     7381 atatcaattc aatatcctgc atcaggaggg caagaaccgg aaagcagaat gatagagccg
     7441 gttctgttgc aaagttttaa ataaagaata aaatccc
//
LOCUS       DAXWLB010000414         3660 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.205, whole genome shotgun sequence.
ACCESSION   DAXWLB010000414 DAXWLB010000000
VERSION     DAXWLB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.205"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            114..806
                     /locus_tag="ACGE4H_003029"
     CDS             114..806
                     /locus_tag="ACGE4H_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFF8366337.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1133..1630
                     /gene="dfrG"
                     /locus_tag="ACGE4H_003030"
     CDS             1133..1630
                     /gene="dfrG"
                     /locus_tag="ACGE4H_003030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFF8366338.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1702..3654)
                     /locus_tag="ACGE4H_003031"
     CDS             complement(1702..3654)
                     /locus_tag="ACGE4H_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFF8366339.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1249 a          594 c          637 g         1180 t
ORIGIN      
        1 gtgtttgata gtatttatat taaaattaca tatgtaattt aaaaacgctt gacattgtgt
       61 ttgagatcac ttacttttag catgtaaaag tattggatta aggagctatg caaatgaaaa
      121 agaaatttat tactggtggg attgtcgttg catttctatg tctaattggc tacacaggca
      181 cgcggtacgc aaatgaaatt attttgtggg gtggagagac agatattgat acaatcaatg
      241 agaatctgac acagcttgat catacattga cagataaaga gcaagcgatt acagatctta
      301 ccgctcgact agcacaaaaa gaacagacat tgcaagattc aaaggcagat gtccaagaat
      361 accaaacaaa agtaaatgaa ttagaaaacc aaaaaaatca atcggcagca gaaaaacaaa
      421 acttagaaag tcaattagtc agcaaggatg cacaactaca aagcaaacaa aatgaaatca
      481 atgtcaaact agatgagatc aaccaaaaaa atgatgaaat caatcatctg aatgcagatt
      541 ggacagcaag actacaagaa gcacaagcaa aactttcaga attacaaaat actgtaaatg
      601 gtttgaatca acaaatcgca acactgacac aagaaaaaac aaatgtagaa actcagttaa
      661 cagatacaca aaagaaatta gacgaagcaa ctgctgaaaa ctcaagcttg aaacaatata
      721 ttcaaaagtt agaaaaagca aaagatgaag tggaagatac agctgataaa tctcagcaaa
      781 ttgttgaaga acatttgcgt aaataatctt tcttaacaag taaacctaaa ttagtaaaga
      841 agcaacagta atctatacta agtagttcag ttttggagta caaaatgaca aaatgtactt
      901 caaaatggac tacttttttc attttaactt acataccaaa ttacatacaa aatgatttat
      961 aaaatgcaat tgaaaatgat aattgaatga aaatttgtga ttttgtggta ttatgataat
     1021 ataacttata gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt
     1081 tgatagaaat aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt
     1141 ttctttgatt gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg
     1201 gaggattccc aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt
     1261 aggtaggaag aaccttgaat caatcggaag agccttacct gacagaagaa atattattct
     1321 gacgagagat aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt
     1381 ttttgagtta tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa
     1441 tttgtttttc ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg
     1501 agatactttt tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg
     1561 gataaagaat gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt
     1621 attgagttaa cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg
     1681 gattactcca agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt
     1741 atctgtaatc cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg
     1801 gcgacaaaag ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt
     1861 gaagttagcg taaatatagc tcttcttatc accacgagct gttgttaatg gtctttcaag
     1921 agatgatata taacgtctta tctgttgaat aaagctattg gtcgaatggt cattaacatt
     1981 taatagcatc tttgcgattt cgtttggttc cagagcagat aaatccgttc tacaatcaac
     2041 tgaataaaat cctttatcct tagtggctaa tggatgtttg atagggttat cggcatattt
     2101 gcgatatctt tctccatctt tactaatagc ctcttcgtgg aattgatgac ttgttagtaa
     2161 ttcagttaga taatgagatg ccaaagtccg tagaaacttt gttttgaatc ctctgatatc
     2221 tccccaatcg agcagttctg cttttgcttg tccgaattct tttaagcatt gttttctgct
     2281 tttcgtcttg ttaacttggc aaataaaatg atgtgcatcg ctcaacctta attctttcgt
     2341 gaatactcta taaaaggcgt ttctaataga gaagtcatca tcactaatca ttcgccattc
     2401 gtcggcatta accatttgtt taattaacca atagtgagca gttgtagtat aaggagcatc
     2461 aacatttaaa ccatcgacaa atctactcct attagtaatt ttccctaatt cgtgaagata
     2521 agcattccta ttttcgctat cgttagcgga tggttcttgt ggataataag accaatctaa
     2581 cctatcattt ttacgactaa aagtattgag atggtcttct ttgaattttc ttgtatcttc
     2641 atttaattca tccattgaaa tattccaatc ataggcaaca tcagaacgga acacatatct
     2701 tgataagacc tcagcagata caactatata cgtttgcata ttaaggtctt caaaccccgt
     2761 atatttcttt gaaccttgtc ctttcttacg gacgttattt aggtagtagt gcatcttgtc
     2821 cgtattaagc cacatctcgt taaatttctt tgtttgaagg ggttgtgtct catatcgttc
     2881 taaaaactcc aaacaacgcc tatacagcca ctccagctta tgatagtagg ttccaactcc
     2941 aattcctaaa atatcacaag tacgactgac tgaaaccctt ccaacaacca tcttagcgaa
     3001 cattggaaga atggtgttct tttgttggtg ataagtagtg gtttcttctc tttttggtag
     3061 aacattagtg aacttcttac acgccttaca ttgatagcgt tgagatttgc ccctactttt
     3121 cccccttttg taaaattgct tcggctcatt gaatggggtt gattcttctt ccgagcagcc
     3181 ttctttatga aattgataat ctggctcaac atcttggatg ctattgattt ctataaggcg
     3241 tttaatttct tcaacaacag accaattaga taaaggggtt actgtacagt tttgcgatac
     3301 acctctgcca ataggattgt cattgcagta cataccttta tgacctttat ctttggaact
     3361 accagtcatt ttatatcgac ttggtttccc ttttacatct ttgaatttgt gctgttcttt
     3421 cccaaaatta caacaaaacg gattgatgca gtaattatat tgaatgggat attcagaacc
     3481 gttccattta aaagacgttg gcaagaacaa cattttgtta taccttgttg caaatcgttt
     3541 tggagttaat aaatcatatt gtttagccct atcttttaat tcttcgtttg aaatcggaat
     3601 tttaacatct acaatttcat cttccttcgt ggctaatctt ttaagcttgg tcattgtcta
//
LOCUS       DAXWLB010000415         3648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.206, whole genome shotgun sequence.
ACCESSION   DAXWLB010000415 DAXWLB010000000
VERSION     DAXWLB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.206"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            105..1226
                     /locus_tag="ACGE4H_003032"
     CDS             105..1226
                     /locus_tag="ACGE4H_003032"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8366340.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1484..2986
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_003033"
     CDS             1484..2986
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8366341.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3053..3580
                     /locus_tag="ACGE4H_003034"
     CDS             3053..3580
                     /locus_tag="ACGE4H_003034"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8366342.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1243 a          624 c          817 g          964 t
ORIGIN      
        1 atgagttccc ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa
       61 aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg
      121 caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg
      181 gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata
      241 ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc
      301 tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg
      361 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
      421 ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag
      481 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
      541 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
      601 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
      661 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
      721 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
      781 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
      841 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
      901 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
      961 aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg
     1021 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
     1081 gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
     1141 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
     1201 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
     1261 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
     1321 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     1381 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     1441 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     1501 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     1561 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     1621 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     1681 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     1741 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     1801 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     1861 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     1921 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     1981 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     2041 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     2101 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct
     2161 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     2221 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     2281 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     2341 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     2401 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     2461 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     2521 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     2581 ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta
     2641 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     2701 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     2761 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     2821 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     2881 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     2941 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     3001 agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta
     3061 tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga
     3121 tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga
     3181 tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc
     3241 acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat
     3301 ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt
     3361 agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt
     3421 tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa
     3481 tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac
     3541 attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta
     3601 aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttag
//
LOCUS       DAXWLB010000416         3648 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.207, whole genome shotgun sequence.
ACCESSION   DAXWLB010000416 DAXWLB010000000
VERSION     DAXWLB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.207"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            105..1226
                     /locus_tag="ACGE4H_003035"
     CDS             105..1226
                     /locus_tag="ACGE4H_003035"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFF8366343.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1484..2986
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_003036"
     CDS             1484..2986
                     /gene="eat(A)"
                     /locus_tag="ACGE4H_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFF8366344.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3053..3580
                     /locus_tag="ACGE4H_003037"
     CDS             3053..3580
                     /locus_tag="ACGE4H_003037"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFF8366345.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1242 a          624 c          818 g          964 t
ORIGIN      
        1 atgagttccc ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa
       61 aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg
      121 caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg
      181 gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata
      241 ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc
      301 tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg
      361 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
      421 ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag
      481 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
      541 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
      601 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
      661 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
      721 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
      781 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
      841 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
      901 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
      961 aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg
     1021 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
     1081 gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
     1141 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
     1201 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
     1261 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
     1321 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     1381 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     1441 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     1501 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     1561 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     1621 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     1681 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     1741 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     1801 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     1861 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     1921 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     1981 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     2041 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     2101 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaggaag tcagtaggct
     2161 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     2221 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     2281 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     2341 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     2401 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     2461 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     2521 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     2581 ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta
     2641 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     2701 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     2761 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     2821 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     2881 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     2941 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     3001 agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta
     3061 tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga
     3121 tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga
     3181 tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc
     3241 acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat
     3301 ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt
     3361 agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt
     3421 tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa
     3481 tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac
     3541 attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta
     3601 aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttag
//
LOCUS       DAXWLB010000417         2153 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.244, whole genome shotgun sequence.
ACCESSION   DAXWLB010000417 DAXWLB010000000
VERSION     DAXWLB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.244"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..237
                     /locus_tag="ACGE4H_003038"
     CDS             <1..237
                     /locus_tag="ACGE4H_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFF8366346.1"
                     /translation="NDVVKVDSSLAAMQFMLVARDHGYETNPIGGFEADQLAEAFGLD
                     KDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            591..2069
                     /gene="msr(C)"
                     /locus_tag="ACGE4H_003039"
     CDS             591..2069
                     /gene="msr(C)"
                     /locus_tag="ACGE4H_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFF8366347.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          751 a          349 c          458 g          595 t
ORIGIN      
        1 aacgatgtag tcaaagtcga ttctagctta gcggctatgc aattcatgtt agttgcgcgt
       61 gatcatggct atgaaacaaa tccaattggt ggattcgaag cagatcagtt ggcagaggca
      121 tttggtttgg ataaagaccg ttatgttcca gttattattt tatcaatagg aaaagcggta
      181 gaagaaggtt acgagtctgt tcgtttagca ccagaaaaga tcactacgtt cgaataaaag
      241 gttttcagtt actattcagt agctattgct ttttttgaat cgtgtgtggt aacctaataa
      301 aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt ttgaataaca
      361 actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt atgtccattt
      421 tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta gtcatagtta
      481 tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt tttctgctct
      541 tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat atggaaaatt
      601 tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa ctatctattg
      661 attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat ggtgtcggta
      721 aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg aaaatccaaa
      781 cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca aaggacctaa
      841 atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag cggaaatgga
      901 gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat gaacaaggga
      961 tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga ctgattgaag
     1021 agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat tttctagatg
     1081 agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt tcggggaatt
     1141 atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga gaagcagagt
     1201 cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg
     1261 aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagaccg gatcggttgt
     1321 cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg aaaacattag
     1381 agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt
     1441 ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga caaaatgtcc
     1501 aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt tctttaggta
     1561 aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta gaacatatcc
     1621 gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta tatcagcaaa
     1681 agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga caaacagagt
     1741 tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag gaaactttga
     1801 cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt gctttgcttt
     1861 ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt gatatggcaa
     1921 cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg tttacttctc
     1981 atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa gggcagaaaa
     2041 taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt gacaaattta
     2101 aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac aac
//
LOCUS       DAXWLB010000418         1972 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.258, whole genome shotgun sequence.
ACCESSION   DAXWLB010000418 DAXWLB010000000
VERSION     DAXWLB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.258"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            89..784
                     /gene="vanR-A"
                     /locus_tag="ACGE4H_003040"
     CDS             89..784
                     /gene="vanR-A"
                     /locus_tag="ACGE4H_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFF8366348.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            762..1916
                     /locus_tag="ACGE4H_003041"
     CDS             762..1916
                     /locus_tag="ACGE4H_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFF8366349.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          646 a          371 c          432 g          523 t
ORIGIN      
        1 ttagtcattt attatgtaaa tgcttataaa ttcggcccta taatctgata aattattaag
       61 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      121 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      181 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      241 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      301 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      361 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      421 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      481 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      541 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      601 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      661 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      721 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      781 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
      841 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
      901 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
      961 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1021 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1081 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1141 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1201 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1261 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1321 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1381 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1441 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1501 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1561 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1621 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1681 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1741 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1801 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     1861 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     1921 tgtatataat tttggctctt tgtcaaatga tgttggtaaa gagaaatctt ag
//
LOCUS       DAXWLB010000419         1082 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.314, whole genome shotgun sequence.
ACCESSION   DAXWLB010000419 DAXWLB010000000
VERSION     DAXWLB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.314"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            38..>1082
                     /gene="tet"
                     /locus_tag="ACGE4H_003042"
     CDS             38..>1082
                     /gene="tet"
                     /locus_tag="ACGE4H_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tet(L)/Tet(K)/Tet(45) family tetracycline efflux
                     MFS transporter"
                     /protein_id="HFF8366350.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTELGRSRPSSI"
BASE COUNT          310 a          161 c          222 g          389 t
ORIGIN      
        1 taaagatttg aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag
       61 ggttaaatac aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa
      121 tgaaatggtt ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc
      181 gagtacaaac tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata
      241 tggaaagcta tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa
      301 ttgtttcggg tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc
      361 tcgttttatt caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc
      421 gcgctatatt ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc
      481 catgggagaa ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc
      541 ctatcttcta ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt
      601 aaagaaagaa gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt
      661 aggcattgta ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag
      721 cgtgctgtca ttcctgatat ttgtaaaaca tatcaagaaa gtaacagatc cttttgttga
      781 tcccggatta gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt
      841 tggaacagta gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct
      901 aagtactgcc gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt
      961 cggctacatt ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg
     1021 agttacattt ctttctgtta gctttttaac agaattaggc agaagtcgcc catcctctat
     1081 ag
//
LOCUS       DAXWLB010000420          871 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.335, whole genome shotgun sequence.
ACCESSION   DAXWLB010000420 DAXWLB010000000
VERSION     DAXWLB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.335"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            <1..825
                     /locus_tag="ACGE4H_003043"
                     /note="aph(2'')-Ia"
     CDS             <1..825
                     /locus_tag="ACGE4H_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFF8366351.1"
                     /translation="EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          377 a           58 c          142 g          294 t
ORIGIN      
        1 gaaataatcg gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt
       61 aaaacaaaat ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat
      121 tttttaaata caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt
      181 agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa
      241 atttattcta ctatgtcaga agaagaacaa aatttgttaa aacgagatat tgccagtttt
      301 ttaagacaaa tgcacggttt agattataca gatattagtg aatgtactat tgataataaa
      361 caaaatgtat tagaagagta tatattgttg cgtgaaacta tttataatga tttaactgat
      421 atagaaaaag attatataga aagttttatg gaaagactaa atgcaacaac agtttttgag
      481 ggtaaaaagt gtttatgcca taatgatttt agttgtaatc atctattgtt agatggcaat
      541 aatagattaa ctggaataat tgattttgga gattctggaa ttatagatga atattgtgat
      601 tttatatact tacttgaaga tagtgaagaa gaaataggaa caaattttgg agaagatata
      661 ttaagaatgt atggaaatat agatattgag aaagcaaaag aatatcaaga tatagttgaa
      721 gaatattatc ctattgaaac tattgtttat ggaattaaaa atattaaaca ggaatttatc
      781 gaaaatggta gaaaagaaat ttataaaagg acttataaag attgattata taatatatga
      841 aaagctatta taaaagacat tagtattaaa t
//
LOCUS       DAXWLB010000421          831 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.338, whole genome shotgun sequence.
ACCESSION   DAXWLB010000421 DAXWLB010000000
VERSION     DAXWLB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.338"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            218..766
                     /gene="aac(6')"
                     /locus_tag="ACGE4H_003044"
     CDS             218..766
                     /gene="aac(6')"
                     /locus_tag="ACGE4H_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFF8366352.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          296 a          124 c          178 g          233 t
ORIGIN      
        1 atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta
       61 ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa ataatgaaag
      121 aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa
      181 ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg
      241 taataatcca gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga
      301 atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt
      361 agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat
      421 cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg
      481 tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta
      541 tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca
      601 tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga
      661 aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga
      721 tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt
      781 gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact t
//
LOCUS       DAXWLB010000422          766 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.351, whole genome shotgun sequence.
ACCESSION   DAXWLB010000422 DAXWLB010000000
VERSION     DAXWLB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.351"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            complement(<1..233)
                     /locus_tag="ACGE4H_003045"
     CDS             complement(<1..233)
                     /locus_tag="ACGE4H_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFF8366353.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSK"
     gene            326..675
                     /locus_tag="ACGE4H_003046"
                     /pseudo
     CDS             326..675
                     /locus_tag="ACGE4H_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT          256 a          143 c          103 g          264 t
ORIGIN      
        1 tgtttagaaa agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa
       61 tgttctatcg aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca
      121 tccgctctca tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt
      181 agtaacaaga tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata
      241 ctccttctta tatttcctaa tagagtataa catagcttta agcattactt aaagataact
      301 taaaagttat cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta
      361 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
      421 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
      481 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
      541 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
      601 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
      661 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
      721 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaa
//
LOCUS       DAXWLB010000423          659 bp    DNA     linear   BCT 11-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate id11
            SAMN40749831-rid21413073.guided.365, whole genome shotgun sequence.
ACCESSION   DAXWLB010000423 DAXWLB010000000
VERSION     DAXWLB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40749831
            Sequence Read Archive: SRR28555151
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002134761.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002134761.1
            Genome Coverage       :: 98x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/10/2024 17:42:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,047
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,900
            CDSs (with protein)               :: 2,900
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 43 of 92
            Pseudo Genes (incomplete)         :: 41 of 92
            Pseudo Genes (internal stop)      :: 23 of 92
            Pseudo Genes (multiple problems)  :: 13 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40749831-rid21413073.guided.365"
                     /isolate="id11"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Austria"
                     /collection_date="2022-08-11"
     gene            37..>659
                     /gene="aac(6')-Ie"
                     /locus_tag="ACGE4H_003047"
     CDS             37..>659
                     /gene="aac(6')-Ie"
                     /locus_tag="ACGE4H_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFF8366354.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEI"
BASE COUNT          277 a           50 c          118 g          214 t
ORIGIN      
        1 gtatatgtat atgattatga aaaaggtgat aaataaatga atatagttga aaatgaaata
       61 tgtataagaa ctttaataga tgatgatttt cctttgatgt taaaatggtt aactgatgaa
      121 agagtattag aattttatga tggtagagat aaaaaatata cattagaatc attaaaaaaa
      181 cattatacag agccttggga agatgaagtt tttagagtaa ttattgaata taacaatgtt
      241 cctattggat atggacaaat atataaaatg tatgatgagt tatatactga ttatcattat
      301 ccaaaaactg atgagatagt ctatggtatg gatcaattta taggagagcc aaattattgg
      361 agtaaaggaa ttggtacaag atatattaaa ttgatttttg aatttttgaa aaaagaaaga
      421 aatgctaatg cagttatttt agaccctcat aaaaataatc caagagcaat aagggcatac
      481 caaaaatctg gttttagaat tattgaagat ttgccagaac atgaattaca cgagggcaaa
      541 aaagaagatt gttatttaat ggaatataga tatgatgata atgccacaaa tgttaaggca
      601 atgaaatatt taattgagca ttactttgat aatttcaaag tagatagtat tgaaataat
//
